LOCUS       DASTEY010000001         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_791093, whole genome shotgun sequence.
ACCESSION   DASTEY010000001 DASTEY010000000
VERSION     DASTEY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791093"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFO72_00005"
     CDS             complement(<1..477)
                     /locus_tag="VFO72_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477691.1"
                     /translation="MNDRAKTTTLALVVVIGMLATFALARVVDARRPPVDLTAADESL
                     YLNAKTARRISLGFNGLAADWYWMRSLQYVGRKIIAQDENAPLDDLSQLNLKLLAPLL
                     ENATTLDPQFLDPYEYAAIVLPAIDVDEAIRITRKGIDANPNAWRLYQHLGYIYWQQ"
     gene            complement(487..1323)
                     /locus_tag="VFO72_00010"
     CDS             complement(487..1323)
                     /locus_tag="VFO72_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477692.1"
                     /translation="MDTTASIAQSNTRAQASQASSWRIIAIARNAFREAVRDRVLYNL
                     VLFVLLLTAAAIFIGELSGGQERKIIVDLGLSAMLLFGVFIAIFVGVGLVYKEIERRT
                     IYAIFSKPVGRGEFLVGKYLGLCLTLLVNVVVMGLGVSLALIYVSRGWDPLIVNLWPA
                     VLLIYIELTILTAVALLFSAFSSPALSALLTFFIFIIGHFSADLKALSVSLGSTSARW
                     LFGALYYLLPNLANYSFITPTAHGHAPSAGFVAAAGLYAIVYITVILAVAMLVFSRRN
                     FK"
     gene            complement(1412..1903)
                     /locus_tag="VFO72_00015"
     CDS             complement(1412..1903)
                     /locus_tag="VFO72_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9477693.1"
                     /translation="MQSKRNNQGFSLIELLIVISVIVIIAAIGVPSLLSARRAANEGS
                     AQSSLRTIHSAQATYQQTSGLGNFGSLNELLNARLIDPALGAGQKSGYIFVVTETDLV
                     AGTSPARFKVTANPQVTSGASQSGTRKFGTREDGVMHGQTDALDTPLTDAELALLTNV
                     MGN"
     gene            complement(1984..2766)
                     /locus_tag="VFO72_00020"
     CDS             complement(1984..2766)
                     /locus_tag="VFO72_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9477694.1"
                     /translation="METRPVKPPAASVISLLPESVICPLPQIVRASATSVSPIKISIK
                     NDRFSALSTETFIGTLVVLSHTDTSRFARLTVNESRWREKFQLTPISFKEHQTRMTTK
                     RQEGFSLIELLIVVAIIGIIAAIAIPNLLASRRAANEGSAQSSLRTIHSSEATYQATT
                     GNGNFASMAELVGANLIDAVLGSNQKSGYTFVVTETDLVAGVSLATFKATCIPTVPAG
                     VSQSGTRKFGIRDDGVMHGQTDALDTHLTDAELALTTNVLGN"
BASE COUNT          699 a          777 c          911 g          668 t
ORIGIN      
        1 ttgttgccag tagatgtagc cgaggtgttg atagagccgc catgcgtttg gatttgcgtc
       61 gatacctttg cgcgtgatcc gaattgcttc atcgacgtca atcgcgggta atacgatagc
      121 ggcatattcg tacggatcaa gaaactgcgg atcgagtgtg gtggcgtttt ccagtagcgg
      181 ggcgagcagt ttcagattga gctggctcaa gtcatccagc ggcgcgtttt cgtcttgtgc
      241 aatgatcttt ctgccgacgt attgcagcga acgcatccag taccaatcag cagccaaacc
      301 gttaaacccg agactgattc gccgcgcggt cttcgcgttc agataaagac tttcatctgc
      361 ggcggtgaga tctactggcg gacgccgcgc gtcaacaacg cgagccagcg caaacgttgc
      421 gagcattccg atcacaacga ccagtgctag tgtggttgtt ttggcgcgat cattcatgac
      481 tacgtttcac ttaaagtttc gccggctgaa cacaagcatg gcgacagcca aaatcacggt
      541 gatgtagaca atcgcgtaga gaccggcagc ggcgacgaac cctgctgagg gtgcgtgacc
      601 gtgtgcggtt ggagtgatga agctgtagtt ggcgaggtta ggaagcaggt aatagagcgc
      661 gccgaacagc cagcgcgcgg aggtgcttcc gagtgacacc gagagtgcct tcagatcggc
      721 gctgaagtga ccgatgatga aaatgaaaaa cgtcaacaga gctgacagcg cgggtgaaga
      781 gaaggccgaa aacagtagcg cgacggccgt cagaatcgtc agttcgatgt agatcaagag
      841 aacagcgggc cagagattaa cgatgagtgg atcccaaccg cggctcacgt aaatcaacgc
      901 cagtgaaaca ccaaggccca tgaccacgac gttgacgagc aacgtcaagc acaggcccag
      961 atacttaccg accagaaact caccgcggcc aacgggcttg gaaaagatcg cgtagatggt
     1021 cctgcgttct atctccttgt agacgaggcc gactccgacg aagatagcga taaaaactcc
     1081 gaacaacagc atcgcgctga gtccgagatc gacaatgatt ttgcgctctt gtccgcccga
     1141 caactcaccg atgaagatcg cggcagccgt cagcagcagc acaaacagca cgagattgta
     1201 gagcacacga tcgcgcactg cttcgcgaaa agcgttacgc gcgatcgcaa ttatgcgcca
     1261 ggagcttgcc tggctggcct gtgcacgagt gtttgattgt gcgattgatg cagtggtgtc
     1321 cacgccgttg taacgctcga tcgtagatga gcgtgagata caactttata ttaatcgtaa
     1381 attttgggaa acaggcgagt ttgctaaccg gtcagttgcc cataacgttt gtgaggagcg
     1441 ccaattcggc atccgtgagc ggggtgtcaa gcgcatcggt ttggccgtgc atcactccat
     1501 cttcgcgagt cccgaacttg cgcgtgccgc tctgcgacgc gccgctggtg acctgaggat
     1561 ttgcggtcac tttaaaccgg gccggtgaag tgcctgccac gaggtccgtt tccgttacga
     1621 cgaagatgta gccgcttttt tgaccggcgc cgagagcagg gtcaatcagt cgtgcgttaa
     1681 ggagttcgtt cagcgaaccg aaatttccca acccggaagt ctgttgataa gtcgcttgcg
     1741 cgctgtgaat ggttcgcagt gatgattgtg ccgaaccctc attcgctgcc cgtcgcgcag
     1801 ataacagact agggactccg atcgcagcga tgatgacgat aaccgaaatt acgatcagca
     1861 gttctatcag cgagaagccc tgattgtttc ttttggattg catggcgtgt cgaggggaca
     1921 ttgggggttt tagccgaaaa gggcgggact gctcccgccc ttctggaatg ttggtgaggc
     1981 ttattagtta ccgagaacgt tggtggtcaa ggcgagttca gcgtcggtca agtgggtgtc
     2041 caaagcatcg gtctgtccgt gcatgacgcc gtcatcacga attccgaact tgcgcgtgcc
     2101 gctctgagaa acgcccgccg gaaccgtcgg aatgcaagtg gccttgaaag tagcgagcga
     2161 tacaccggcg acgaggtctg tttcagttac gacgaaggtg taaccgctct tttgattgga
     2221 tccgagaacg gcatcgatca ggttcgcgcc gaccaactca gccatagaag cgaagttgcc
     2281 attaccggtg gttgcctgat aggtagcttc agaactgtga atggtgcgaa gggacgactg
     2341 cgccgaacct tcattcgcgg cacggcggga agcaagcagg ttggggatag cgatggcggc
     2401 gatgatgccg ataatcgcga cgacgataag aagttcgatc aacgaaaagc cttcttggcg
     2461 tttggtagtc attctcgttt ggtgctcctt aaaagatata ggggttaact gaaatttctc
     2521 gcgccacctg ctttcgttca ctgtgagccg cgcaaagcgc gaagtgtcgg tgtggcttag
     2581 aacaaccagt gtgccaatga aggtttcggt tgaaagcgca gaaaagcgat cgtttttgat
     2641 cgaaattttg atcggggaga ccgaggtcgc agaggccctg acaatttgtg gcagcgggca
     2701 aatgacgctt tccggcagga ggctaatgac gcttgcggca ggaggcttta caggtcgcgt
     2761 ttccaagtcg cgcccggctt ctctcagtca cacctggctt cagccaggtg ctggttcgtt
     2821 ctctaaaact ggaaaccgtt tcaacggttt ccagaaattg cgcgtcattg aacgcgggta
     2881 aaagcgaagt gtgactggga gttggtttag tttccttcgc cggcgccggc ggcaaactcg
     2941 ccaccgctgt cttcctcgcg ccggcgtggg ccttgatcgc ccgtccggcg ccgcggagta
     3001 gactctgggc tgcgacgctc gtcgcgcatc tgcgccgtca gaccacgaac accgt
//
LOCUS       DASTEY010000002         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_955901, whole genome shotgun sequence.
ACCESSION   DASTEY010000002 DASTEY010000000
VERSION     DASTEY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955901"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4021
                     /locus_tag="VFO72_00025"
     CDS             <1..4021
                     /locus_tag="VFO72_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HET9477695.1"
                     /translation="VKAEAVLEDVELFDASFFGFTPREASTLDPQHRIFLEESWAALE
                     SAGYNSETYRGRIGVFAGESMNSYFLHNLYPNRKLLNSLGPAQVVIANDRDYLATQVS
                     FRLNLKGPSLSIQTACSTSLVAVHLACQSLLNRECDMALAGGVSISVPQGLGGHYQEG
                     GIISPDGHCRAFDANARGTVKGSGAGVVVLKRLADALDDRDTIHAVIKGSAINNDGAM
                     KVGYTAPAVEGQAIVIEEALALSSVEPETIGYVEAHGTGTALGDPVEIAALTQAFCSG
                     TEATGFCAVGSVKTNIGHLDAAAGVAGLIKTILALEHKQIPPSLHFEHPNPNIDFANS
                     PFFVNTELRRWDTDGRPRRAGVSSFGIGGTNAHVVLEEAPASSSVDSSGSPHLLVLSA
                     RTQNALAQASTNLANHLEKRPGLDLADVAYTLSAGRREFEHRLAVVCRDRADSIATLS
                     ALNRSRVSIGAARYEKRPVAFMFPGQGAQHVGMGDALYEREPVFSGELDHCAELLRSR
                     LGVDLREIIYANDLPSAEAAARLAETRFAQPALFAIEYALAKLWIEWGVVPQAMIGHS
                     IGEYVAACLAGVFSLEDALRLVTARGEIMQRLPRGAMLTVSLTPEEAEPLLNEQLSLA
                     AVNSPSLCVLSGTIEAIDQLERRLSSEGVICRRLHTSHAFHSVMMEPALEEFTALVRS
                     VERRAPQLPYLSNVTGKWITEAEAVDPSYWARQLREAVQFSAGMAELLNDPAIVLLEV
                     GPGQTLATLASQQPHDPLDRMAFASLRSPKDEQSEIELFLKTVGRLWLAGVEIDWSRF
                     YAGQPRWRVQLPTYPFERQRYWIDPPSDVEAPFTMAAGEDVSEDLTSRENVTVFKDVE
                     TFSTVAEVETEVIEIWRELFGIENIRPQDNFFDHGGNSLIALQLVAQLRATFKLDLPL
                     RVVFESQTVAALAVRIFELITREQEIAELDNLLREIESLAPEELPAQPITTAAVRSHE
                     PETRQQPAAQSISFSLYFFSDDGSKVSDDKYRLVLESAKFADQHGFTAVWTPERHFQD
                     FGGLYPNPSVLSAALAVLTERIQIRAGSVALPLHHPVRVAEEWSVVDNLSRGRVAVSF
                     ASGWHTDDFIFAPDTYDDRKDVMFRHIELIQKLWAGEKIKLPSAGGTEVEFRILPRPI
                     QSRLPIWITSSGNAETWRRAGAIGANVLAAYVGYSREELAQRIELYRAARAEHGHDPR
                     AGVVTIMLHTFVGSDDEAVKEKVRAPFSNYLRSYFRQYETTGAAVADVSEADKDALMS
                     AAFEKYFEESTLMGAPDKCARLIDSLVDIGVDEVACLIDFGVDADSVLGSLARLDELR
                     ARYAPSTRAASFAVALHKE"
     gene            4131..>4480
                     /locus_tag="VFO72_00030"
     CDS             4131..>4480
                     /locus_tag="VFO72_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9477696.1"
                     /translation="MKDLSARIAALSPEQRALFEASLQAKGLRVPQVDQIPRRPREDV
                     NLFPTSIDQERLWFIEQLQPGNAAYNIFSASRIRGPLNASLMELVVNELIKRHEVLRT
                     TFTIVDDQPMQIIHP"
BASE COUNT          955 a         1234 c         1303 g          988 t
ORIGIN      
        1 cgtgaaggcc gaagctgtcc ttgaggacgt cgaattgttc gacgcctcgt tcttcggctt
       61 cactcctcgt gaagcgtcga cactcgaccc tcagcatcgc atctttctcg aagagtcgtg
      121 ggcggcgctg gaaagtgctg gttacaactc agagacttac cgcggccgca tcggcgtctt
      181 cgcgggtgag agcatgaact cttacttcct gcacaatctt tacccgaacc gcaaacttct
      241 gaattcactt ggaccagcgc aagttgtcat tgcaaacgac cgcgactatc tggctacgca
      301 agtctctttt cgtttgaact taaaggggcc gagtctaagc attcagaccg cgtgctccac
      361 gtcgctcgtg gccgttcatc tcgcctgcca aagtttgctg aaccgcgaat gcgatatggc
      421 gctcgcggga ggcgtttcga tcagtgtgcc gcaaggactc ggcggccatt atcaggaagg
      481 cggaataatc tcgcccgacg gccactgtcg cgcgttcgac gcgaatgcac gtggtacggt
      541 gaagggaagt ggcgccggtg tcgttgtatt gaaacggctc gcggacgcat tagacgatcg
      601 cgacacgatt cacgcggtca tcaaaggttc ggcgattaac aacgacggcg cgatgaaggt
      661 tggttacacg gcgccggcag tcgaaggaca ggcgatcgta attgaagagg cgctcgcgct
      721 ctcgtcggtc gagcctgaga cgatcggata cgtagaagcg cacggaacag gcacggcgtt
      781 aggcgatcca gtggagatcg cggcgctgac gcaagcgttt tgctcaggca ctgaagcgac
      841 ggggttctgc gccgttggct cagtaaagac caacatcgga caccttgatg ccgctgcggg
      901 tgtcgcgggt ctcatcaaaa ccatcctggc gctggagcac aaacagattc cgcctagtct
      961 gcactttgag caccccaacc caaacatcga cttcgccaac agtcccttct tcgtcaacac
     1021 agagttacgc cgttgggata cagatggaag gccgcgacgg gcgggcgtta gttcattcgg
     1081 catcggcgga acaaacgctc acgttgtgtt ggaagaggcg cctgcaagct caagtgtcga
     1141 ctcgtccggt tcacctcatc ttctggtctt gtcggcgcgg acacaaaacg cgctggccca
     1201 ggcgagcacg aatctcgcaa atcatcttga gaaacgccca ggacttgacc ttgccgacgt
     1261 tgcctatacg ttaagcgcag gcaggaggga atttgagcat cgtctggctg tcgtgtgtcg
     1321 cgatcgggct gactccatcg caacgctctc tgcgctcaat cgttcgcgcg tttccatcgg
     1381 cgcggcgcgg tatgagaaac ggcccgttgc gttcatgttt ccgggacagg gggcgcagca
     1441 tgtcggaatg ggagacgcgt tgtacgaacg tgagccggta tttagtggtg agttggacca
     1501 ttgcgctgaa cttttgcgga gccggctcgg agtagacctg cgagaaatca tttacgcgaa
     1561 cgacctacca tcggcagaag ctgctgcccg tcttgccgaa acacgttttg cgcagccggc
     1621 gctattcgcc atcgagtatg cgttggcaaa actttggatt gagtggggag tcgtgccgca
     1681 agcgatgatt ggacacagca tcggtgagta cgtcgctgct tgtctggccg gtgtcttttc
     1741 tcttgaggat gcgctgcggc tggtgactgc gcgcggagag atcatgcagc ggctgccacg
     1801 tggcgcgatg ctcacggttt cgcttacgcc ggaagaagct gagcctctat taaacgaaca
     1861 gctctcactt gcagccgtca actcaccttc tctgtgtgtt ctatccggca cgattgaagc
     1921 catcgatcag ttggagcgac gactctcaag cgaaggtgtg atctgtcgtc gtcttcatac
     1981 gtcacacgcg tttcactcgg tgatgatgga accagcgctc gaagagttta cggccctcgt
     2041 gcgctctgtt gagcggcgcg cgccgcagct tccctatctc tccaacgtga ccggcaagtg
     2101 gatcactgaa gcagaagcag tggatccttc ctactgggcg cgacaattgc gcgaggccgt
     2161 tcaattttcc gccggcatgg ccgaattgct caacgatccg gcgatcgtgt tgctggaagt
     2221 cgggccaggt caaacgctgg ctacactggc ttcgcagcaa ccgcatgatc cattggatcg
     2281 catggcgttc gcttcgttac gctcgccgaa ggatgaacaa tcagaaatcg agttgtttct
     2341 gaagacggtt ggtcgcctgt ggctggcagg tgtggaaatc gactggtcca ggttttatgc
     2401 tggtcagccg cgctggcgtg tacaacttcc aacgtatcca tttgagcgcc agcgttactg
     2461 gatcgatccg ccgtccgacg tagaagcgcc attcacaatg gcggccggtg aagacgtcag
     2521 tgaagatctg acgagtcgtg aaaatgtaac agttttcaaa gatgtcgaga cattttccac
     2581 tgtggcggaa gtcgaaaccg aggtcatcga gatctggcgc gaacttttcg ggatcgagaa
     2641 cattcggcct caagacaact tcttcgacca cggcggtaat tcactgatcg cgctccaact
     2701 cgtggctcaa ctccgcgcga ctttcaagct cgatctgccg ttgcgcgtcg tcttcgaatc
     2761 tcaaactgtt gcggcgctgg ctgttcgaat ctttgagctg atcacacgag aacaggagat
     2821 agctgagctt gacaatctgt tgcgtgagat cgagtcgctg gcgcctgaag agttgccagc
     2881 gcaaccgatc accacagccg ctgtgaggtc gcatgaaccc gagaccaggc agcagcctgc
     2941 ggcccaaagc atcagcttca gcctctactt cttctccgac gacggttcca aagtttcaga
     3001 cgacaaatac cggctggtgt tggagagcgc gaagtttgca gaccagcatg gcttcacagc
     3061 cgtctggaca ccggaacgcc acttccagga cttcggtggt ctgtatccga acccttccgt
     3121 cctgagtgcg gcgctcgcag tgttgacgga acgaattcag attcgagctg gcagtgttgc
     3181 cttgccgctt caccacccgg tgcgcgttgc cgaagagtgg tcggtggttg acaacctgtc
     3241 gcgtggccgt gtcgccgtat cgttcgcgtc cggctggcat acggacgatt tcatctttgc
     3301 gccggataca tacgacgacc gcaaagacgt gatgtttcgt cacatcgaat tgattcaaaa
     3361 gttgtgggca ggtgagaaga tcaaactgcc gagtgccggc ggaaccgaag tcgagtttcg
     3421 aatactgccg cggccgatac aatcgcggtt gccgatttgg atcacgagtt ccggcaacgc
     3481 ggagacgtgg cgcagagcgg gcgcgatcgg cgccaacgtt ttggcagcct acgttggata
     3541 ctctcgggaa gagttggccc aacgcatcga gctttatcgc gcggcacgtg cagagcacgg
     3601 ccacgatcca cgcgccggcg tggtgacaat catgctgcac acgtttgtcg gcagcgatga
     3661 tgaggcagtc aaagaaaaag ttcgcgcgcc tttctccaat tacctgcgca gttatttcag
     3721 acagtatgag acaacaggcg ccgccgttgc tgatgtcagc gaagccgaca aagacgcact
     3781 gatgagtgcc gcctttgaga agtacttcga ggagagcact ctgatgggag cgcctgacaa
     3841 gtgtgcgcga ctgattgact cgctcgtgga catcggcgtt gacgaggtcg cgtgtcttat
     3901 cgatttcgga gtcgatgccg attccgtact tgggagtctc gcgcggctgg atgaattgag
     3961 agcgaggtac gctccaagta ccagagctgc ttcgttcgca gtagctttgc acaaagaatg
     4021 aaaatagagt ttcttctctg tgttctctgc gcctctgtgg tcaattctgc aagcgatcca
     4081 gtaaccaccg agacacagag aacacagagg ttgtacaaag aaatcgtttt atgaaagatc
     4141 tttccgcaag gatagcagcg cttagtcccg agcagcgcgc cctctttgaa gcctctctcc
     4201 aggctaaagg actgcgtgta ccgcaagtcg atcagatccc gcgccgcccg cgagaagacg
     4261 ttaacctgtt tccgacttcg atcgatcagg agcgtctctg gttcattgag caactgcagc
     4321 ccggcaacgc ggcttataac attttcagcg ccagccgaat caggggcccg ttgaatgcgt
     4381 cgctgatgga actcgtcgtc aacgagttga tcaagcgcca tgaagttctg cgcacaacct
     4441 tcaccatcgt tgacgatcaa ccgatgcaaa tcatccatcc
//
LOCUS       DASTEY010000003         6087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_33004, whole genome shotgun sequence.
ACCESSION   DASTEY010000003 DASTEY010000000
VERSION     DASTEY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33004"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1266
                     /locus_tag="VFO72_00035"
     CDS             91..1266
                     /locus_tag="VFO72_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9477697.1"
                     /translation="MDRLFQLMKEKNASDMFFAVNSPVHIKINGNLIPINQQKLEPEN
                     ILSLLAEIATPEQMQELEVDNELNMGISVANLGRFRLSAFRQRGSVSAVFRFVPANIP
                     ALGDLGLPPVLSDLIMEKRGLLLLVGATGSGKSTTLAAMIDCINSTRSGHIVTIEDPI
                     EFLHRDKHAFVTQREVDVDTRSFAEALRGALRQDPDVILVGEMRDHETIETALTAAET
                     GHLVLSTLHTLDATETITRIVSSFPSHQQKSVRIQLAGILKAVISMRLVRAAKGAGRV
                     PAIEVLVSTAFIRDHIINEEKTYMIREAIAAGTSQYGMQTFDQSLFHLLQSRLITVEE
                     ALHNATNPDEFKMRVQGIYSADDTLTNMTTPATLTSATPKISSPTIRTEPDSIFVKH"
     gene            complement(1263..2798)
                     /locus_tag="VFO72_00040"
     CDS             complement(1263..2798)
                     /locus_tag="VFO72_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477698.1"
                     /translation="MLQNLTTPASIEAACRQIAGLFQQHAEWFCTAGRDETYALRRDE
                     MDISIAHERLVFSCWTEQGSRSWRVLGWQWNGQMLLLQASRRMGAELPLIELVPRASA
                     AAVAGTIRSAREHRCHKLAQLACASRPETRIERCSLSPGTRPGQPGRYARIILHRKQT
                     RIAVSGPVASSHPASVDAFLSATLLWFKRTADRIKPPFIQQLWLVVPGALLKPLLYRV
                     ALLHEGLRATIHVFAVDDDLTTLREVDPLERRELWIKTLKQFPPVPAAAISEQTSAII
                     ASAPEAIDVVHARHGETLRYFGLPFARVRSLPGTERVWFGIDGARRRLLDESTFSDWQ
                     CLLRGLREHRAASAVDRHHAFYRSAAEAWLESLLRRDITKLDPGLIIAPLHAQFRTAR
                     GGKLGIRPIDLLALRQDGRLVVIELKVYEDREHVLQGADYWRRVEAHRRRGHIARAKL
                     FGDLKIRDEPPLVYLVAPTLRVHPSFATLARCIDCDIEMYRFDINEDWRSGVRVMRRM
                     RVN"
     gene            2952..4133
                     /locus_tag="VFO72_00045"
     CDS             2952..4133
                     /locus_tag="VFO72_00045"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET9477699.1"
                     /translation="MKNIYLAGAVRTPIGKFGGTLQSWTAADMGVAVAKETLRRANVS
                     PDQIDDSIWGCARQAGGGPNVARQITFRAGVPETIPAFTVNQACGSGLKAIILAAQEI
                     MLGRAGAVLAGGTESMSSVPFFAEGARWGMRLGNTQLVDGMYRDGFNDPLSGLMMGQT
                     AEKLARQYEISRAEQDEFALQSQQKAEAAIKSGRFSDEVMSLEITGRKNETTVFAQDE
                     HCRAGTTLTDLAKLPPVFYKDGSVTAGNSSGITDGAAAVVVLSEDGLKRAGVTPHARI
                     VDYEISGVAPDVMGIGPVPAVRALLARQNLTLDQIDLIELNEAFAAQVIACDRELKFD
                     RTRLNVNGGAIALGHPIGCTGVRITTTLVHEMQKRQAKFALATLCVSGGMGLALLLER
                     V"
     gene            4181..4894
                     /locus_tag="VFO72_00050"
     CDS             4181..4894
                     /locus_tag="VFO72_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477700.1"
                     /translation="MLVSFSGCALLLIASACSQNQNVSTDTNTNETVVSSIPPFATRE
                     PERYSAKRTITTVTAAGETVVTKNSIARDGERRRDEHELASQRVVYLTLPEGRFLLLP
                     EQRTYAESTIATPVQTPGNLEETDSSPDRLLHTEPIATSYRRMGAETIGGRATQKYRV
                     IVNSSTDANVSVSESFIWIDEALHMPIRSETTSRDGKRVTMELSDIALDVDGNLFQIP
                     DGYEKITILELRKRLKLIE"
     gene            5000..6004
                     /locus_tag="VFO72_00055"
     CDS             5000..6004
                     /locus_tag="VFO72_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477701.1"
                     /translation="MNRKFLNLAIIVLAVCSSQAFAQTRARNTTPAPAGLLASLPESD
                     AVAHVKVRQLLNEAMPRILANNSAKLAEVNASLENFKTKTGLDARMFEQVALGVRFGY
                     PAEGVTKVQTTALVNGAFSSDAMVAAGRVASNGNYREEKYHDKTIYIFRLDENVRLFG
                     MFNFRISELAAAPLDANILALGDPAGVRAAIDASRSRKRVNAELIALASRDPNAVIGF
                     GGNITEQLIRNLDIGNAPIAADLSKLRQFYGSVGTTEKDLELFLAARAVNADAAKSLG
                     DTLEGMKQFGAFLVGRLSGARGVLAKSALANLKIVTAANELQIRTAVPQADVGPLVGG
                     "
BASE COUNT         1395 a         1761 c         1713 g         1218 t
ORIGIN      
        1 aatggggtct gaccccgatg ttcaaatacc tttggctaaa aagccaaata aaattggggt
       61 ctgaccccga ctctggagac gatgatggcg atggaccgtt tgtttcaact gatgaaggag
      121 aagaacgcct cggatatgtt cttcgcggtg aattcaccgg ttcacatcaa gatcaatggg
      181 aacctgattc cgattaacca gcagaagctg gagccggaga atatcctgtc gctgctggcc
      241 gagatcgcaa cgcccgagca gatgcaggag cttgaggtcg acaacgaact gaacatgggt
      301 atctcggttg ccaacctggg ccgcttccgc ttgtcggcgt tccgccagcg cggctcggtg
      361 tcggccgtgt tccgcttcgt gccggcgaat atcccggccc tcggcgacct tggcctgccg
      421 cccgtgctgt ccgacctgat catggaaaag cgcggcctgc tgctgctggt cggcgcaacc
      481 ggctccggca agtcgaccac actcgccgca atgatcgatt gcatcaactc gacccgcagc
      541 gggcatatcg tcaccatcga agatccaatc gaattccttc atcgcgacaa acacgcgttt
      601 gttacgcaac gcgaagtcga cgtcgataca cgttcatttg cagaagcgtt gcgcggcgca
      661 ctgcgccagg atcctgacgt gattctcgtc ggcgaaatgc gcgaccacga aacgatcgaa
      721 actgctttga ctgccgccga gaccggccac ctcgtgcttt caactctgca cacgctcgac
      781 gcaacagaaa ccatcacccg aatcgtctct tcgtttccgt cgcaccagca gaagtctgtg
      841 cgcattcaat tggccggcat tttgaaagcg gtgatttcga tgcgcctcgt gcgcgccgca
      901 aaaggcgcgg ggcgcgtgcc ggcgattgaa gtgctcgttt cgaccgcatt cattcgcgat
      961 cacatcatca atgaagaaaa gacctacatg attcgtgaag cgatcgccgc gggcacgtct
     1021 caatacggca tgcagacgtt tgaccagtcg ctgtttcacc tgctccagtc gcgtctgatc
     1081 acggtggaag aagcactaca caacgccacc aatcctgacg aattcaagat gcgcgtgcag
     1141 ggaatctact cggccgacga cactctaacc aacatgacta ctcctgcgac actgacatct
     1201 gcgacgccaa aaatctcctc acctacaatc cgcaccgaac ccgacagcat ctttgtgaag
     1261 cattaattca cacgcatgcg acgcattacg cgcacgcccg agcgccagtc ttcgttgatg
     1321 tcgaagcggt acatttcgat gtcgcagtcg atgcagcggg cgagtgttgc gaatgaggga
     1381 tgcacccgca gtgtcggcgc gaccagataa accaatggcg gttcgtctct gatcttcaga
     1441 tcgccgaata gttttgctct tgcgatgtga ccacgacggc gatgtgcttc aaccctgcgc
     1501 cagtaatccg caccctgtaa cacgtgctcc cgatcttcgt aaactttcaa ctcgatcacg
     1561 accagtctgc cgtcctgccg caacgccaag agatcgattg gtcgaatgcc gagcttgccg
     1621 ccgcgtgcgg tgcgaaactg cgcatgcagc ggcgcgatga tcaggcccgg atcgagtttc
     1681 gtgatatcgc ggcgcagcag cgattccaac cacgcttccg cagccgaacg gtaaaaggcg
     1741 tgatgacgat caacagcgga cgccgcgcga tgctcgcgca gaccgcgaag cagacattgc
     1801 cagtcgctga acgtcgactc atccagaaga cgccggcgcg caccatcaat cccaaaccag
     1861 actctttccg tgcccggcaa cgagcgaacg cgcgcgaacg gcaagccaaa gtatcgcagc
     1921 gtttcaccat gacgcgcatg gaccacgtcg atcgcttccg gcgctgatgc gatgatggcg
     1981 ctggtctgtt cggaaatcgc ggcagcaggt acaggcggaa actgtttgag tgtctttatc
     2041 cacagttcac ggcgctcgag aggatctacc tctcttagcg tcgtcagatc atcgtcaaca
     2101 gcgaagacat gaatcgtggc tctcaagcct tcgtgcaaga gcgccacgcg atacagcagc
     2161 ggcttgagta atgcgccggg tacgactaac cagagctgtt ggatgaacgg cggcttgata
     2221 cgatcggcag tccgtttgaa ccacaacagc gttgcagaca gaaacgcatc aactgacgcc
     2281 ggatggcttg aagcgaccgg accgctaacc gcgatccgcg tttgcttgcg gtgcaaaatg
     2341 atgcgggcgt agcgaccggg ttggccgggt cgtgtgccgg gactcaagct gcaccgttcg
     2401 atacgcgttt cgggtcgtga cgcgcaggcg agttgggcca acttgtgaca acgatgttcg
     2461 cgcgcgctgc gaatcgttcc agcaactgcg gcggccgaag cgcgcggaac caactcgatc
     2521 aacggtaact ccgcgcccat ccttcgcgat gcctgcaaaa gaagcatctg cccgttccat
     2581 tgccacccga gaacacgcca cgagcgactt ccctgctcgg tccagcacga aaacaccagc
     2641 cgttcatgcg cgatcgagat gtccatttca tcgcggcgca aggcgtaggt ttcgtcgcgt
     2701 ccggccgtgc agaaccattc cgcgtgttgt tgaaaaagac cagcaatctg ccggcaagca
     2761 gcttcgattg aagcaggagt cgtgaggttc tgaagcattg tgaggtccaa actggacagg
     2821 atttacccaa ttcacaggaa aaatgatggc ttcctgccaa aaggaatcgc aatcctgcaa
     2881 ttctgaaatc ctgtctaacg caaggctttg cgtgaagact tctatcctgt gctaccattt
     2941 ttggtcaagc aatgaagaac atttacctcg caggcgcagt cagaacaccg attggaaaat
     3001 tcggcggcac attgcagtcg tggaccgccg cggacatggg cgtagcggtc gcaaaggaaa
     3061 ctctgcgacg cgcgaatgtg tctcctgatc agatcgacga ctcgatttgg ggttgtgcgc
     3121 gacaagctgg cggtggtcca aatgtcgccc gccagatcac gtttcgagcg ggcgttcccg
     3181 aaacaatacc ggcctttacg gttaaccagg cgtgcggttc cgggttgaaa gcgatcatcc
     3241 tcgcggcgca ggagattatg ctgggacgcg ccggcgccgt gctcgccggc ggtaccgaaa
     3301 gcatgtctag cgttcctttc ttcgccgaag gtgcgcgttg gggcatgcgt ctgggaaaca
     3361 cgcaactcgt cgacggcatg tatcgtgacg gcttcaacga tcctttgtcc ggactgatga
     3421 tgggccaaac ggccgaaaag cttgcgcgcc aatacgagat ctcgcgcgca gagcaggatg
     3481 agtttgctct ccagtcgcag caaaaagcgg aagccgcgat caagtcaggc cgcttcagtg
     3541 acgaagtgat gtcacttgaa atcacgggcc gcaagaacga aacgaccgtc ttcgcacagg
     3601 atgaacactg tcgcgccggc acgacactca cggatctcgc caagttgccg ccggtcttct
     3661 acaaagacgg cagtgttact gccggaaact cgtcaggtat caccgacggc gcagccgctg
     3721 ttgtcgtgct cagcgaagat ggtctcaaac gagcaggagt cacgccccac gcgcgcatcg
     3781 tcgattatga aatctccggc gtagctcctg acgtcatggg gatcggtccg gtccctgctg
     3841 ttcgcgcact gctggcgcgc cagaatctga cgttggacca gatcgatcta atcgaactta
     3901 acgaagcatt cgcggcccag gtcatcgctt gcgaccgcga actgaagttt gaccggacac
     3961 gattgaacgt caacggcggc gccatcgcgc tgggccaccc tatcggctgc acgggcgttc
     4021 gtatcaccac aacactcgtc cacgaaatgc aaaaacgaca agcaaagttc gcgctcgcaa
     4081 cattgtgcgt gagcggcggc atgggtttgg ccctgttgct ggaacgagtg tgagtcgaat
     4141 gccgtcgtgc cggaaagcgc cccgggaaat cacccggaga atgttagttt cgttttctgg
     4201 ctgcgctctg cttctcatcg catctgcctg ttcgcaaaac cagaacgtca gcactgacac
     4261 caacactaac gaaacggtcg tctcttcgat cccaccgttt gcaacgagag aacctgaacg
     4321 ctacagcgcc aagcgcacca tcactacggt aactgccgca ggcgaaaccg tggtaacgaa
     4381 gaattcaatc gcgcgagacg gcgaacgccg tcgcgatgaa cacgaactcg cgtctcagcg
     4441 cgtcgtctat ctcactctgc cggaaggaag gtttctgtta ctgcccgaac aaaggactta
     4501 cgccgaatcg acgatcgcta ctcctgtgca aacacccggc aacctggaag agaccgatag
     4561 ttcgccggat cgcctgctgc acaccgagcc aatcgcgaca agttaccggc gcatgggcgc
     4621 cgaaacgatc ggcggccgcg cgacgcaaaa gtatcgcgta attgttaaca gttccacgga
     4681 cgcgaacgtc agcgtcagtg agagttttat ttggattgat gaagcgctgc acatgcccat
     4741 ccggtcagaa acaacgtcac gcgacggaaa acgcgtcacg atggaacttt cggacatcgc
     4801 gttggacgtc gacggcaatc tattccaaat acctgacggt tatgaaaaga ttacgattct
     4861 cgagctgcgg aagcgtttga agctcatcga gtgaggtgct cagagcaaac cttaggaacg
     4921 ctttcgggca tcgcagtgcg ggctgtataa gccacgccac cccaatctcc cgtaagctaa
     4981 cccgtagaga ggtttcgttg tgaatagaaa gtttctaaat ctagcaatta tcgttttagc
     5041 agtctgtagt tcgcaggcct tcgcgcaaac gcgtgcgcga aacacgacgc cggctccggc
     5101 tggcctgctg gcctcgttgc ctgaatcgga cgcggtcgca cacgtgaagg tgcgacaact
     5161 gctcaacgaa gcgatgccgc gaatactcgc caacaactcc gccaagctgg ccgaagtgaa
     5221 cgcgagtctc gaaaacttta agacgaagac cggtctcgat gcgcggatgt tcgagcaggt
     5281 cgcgctgggc gttcgttttg gctatcccgc cgaaggcgtg acgaaggtgc aaacgaccgc
     5341 gctggtgaat ggcgccttta gttcggacgc gatggttgct gccggaagag tcgcttccaa
     5401 cggaaattat cgcgaggaga aatatcacga caagacgatc tacatcttca ggctcgatga
     5461 aaacgttcgt ctgttcggta tgtttaattt cagaatcagt gaactcgcag cggcgccgct
     5521 tgacgcgaac attctagcgc tcggcgatcc tgccggcgtg cgcgccgcga tcgatgcaag
     5581 ccgttcgcgc aaacgcgtca acgccgaact catcgcttta gcgagtcgcg atccgaatgc
     5641 agtgatcggt ttcggcggca acatcaccga gcaattgatc agaaacctgg acatcggcaa
     5701 cgcgcccatc gctgcggact tgagcaagtt gcgacagttt tatggctcgg ttggaactac
     5761 cgaaaaggac ctcgagttgt ttctggccgc gcgcgcagtg aatgccgacg ccgcgaagag
     5821 tctcggcgac actcttgaag ggatgaagca gtttggtgct tttctcgttg ggcgtttatc
     5881 aggagcgcgt ggagtcttgg ccaagagcgc gctggctaat ctgaagatcg tgacggcagc
     5941 gaacgaacta cagattcgca ctgcggttcc gcaagcggac gttggtccat tggtaggtgg
     6001 ttaatgagtg gtgcttagta cttgaacgcc gaaggcgttc gcaagttcta agcccagggt
     6061 tcataccctg gggacaacga aactcac
//
LOCUS       DASTEY010000004         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_642789, whole genome shotgun sequence.
ACCESSION   DASTEY010000004 DASTEY010000000
VERSION     DASTEY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642789"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFO72_00060"
     CDS             complement(<1..729)
                     /locus_tag="VFO72_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9477702.1"
                     /translation="MNETRRQLLTTLSLAGAGALLIGCGTMSTNRRGAANDDGREPAP
                     GEAVPIEVTATEDLMREHGILRRALLVYQESAIRLRQDPAAMPAEALEKTAQLFRVFG
                     EDYHEKRLEESFVIPLIRKVQGPVAAFADVLVAQHARGREITDYLLSVSKGDRISAGQ
                     VDSVAKALESFVRMYEHHATVEDTVVFPAWKTATGEQEYSELGEKFEEIETEIFGADG
                     FQTALKRMEEIEASLGLTNLDMFTA"
     gene            812..1267
                     /locus_tag="VFO72_00065"
     CDS             812..1267
                     /locus_tag="VFO72_00065"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9477703.1"
                     /translation="MDNQEIARRFYRLSALMEIRGDDPFRLRSYRNAAEAIEVWPTPL
                     KEIAEKEGLAGLQEIPGVGKAIAGKVIDLLERGTFDAWERLTAETPETVLDLTEIPGI
                     GPKTAALLHQRFKVSSLPELKAFVAGGGLEMVDGIGPKTEERIKEALDL"
     gene            1284..2405
                     /locus_tag="VFO72_00070"
     CDS             1284..2405
                     /locus_tag="VFO72_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HET9477704.1"
                     /translation="MTKKIKRRDFMRTGAAAGLTIAATSKQAAGFPSILTQSTVKPLV
                     ISSANGNRFKDGGTMTAVQKAFTMMTSGSDVLDALIAGVNIVELDPLDDSVGYGGLPN
                     AEGIVQLDSSVMHGPKKRAGAVGALEGVRTPSLVARAVMENTDHHLIVGKGAQDFARN
                     MGFKIEEDLNTENSRRRWLEWKRRTDPSHYLDPKKRAEVGHRIALEMLAEGKLREENL
                     HGTINCDGINSKGEICGVTTTSGLAWKIPGRLGDSPILGAGLYVDGAIGAAGSTGRGE
                     ANLFNLCSFVIVEEMRRGAHPKDAGMEVLKRIKNNTIEKRLLNSRGLPNFGISFYILN
                     AKGEYAGVTMYEGGSFAICNDNGPQTLKAEPLLQGRPSD"
     gene            complement(2461..2844)
                     /locus_tag="VFO72_00075"
     CDS             complement(2461..2844)
                     /locus_tag="VFO72_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9477705.1"
                     /translation="MPRVVHFEIHAGDPERAVNFYQKLFGWSFQKWEGPMEYWVVNTG
                     PDDQPGINGGLMRRQGEIDGQAVIAYVCTVDVADVDASLKTALDNGATVALPKMAIPG
                     VGWLVYCKDTEGNIFGMMQNDSAAK"
     gene            complement(2858..3202)
                     /locus_tag="VFO72_00080"
     CDS             complement(2858..3202)
                     /locus_tag="VFO72_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477706.1"
                     /translation="MSKPLLGVVLGAILGVIDGLTAWFTPAVRDQLGGIVIGSTIKGI
                     IAGIAIGFFAKKFQSLPIGILFGVAVGALLAYLTAWMTGGYYFEIIVPGTIVGLIVGF
                     ATQKYGTVPQTS"
BASE COUNT          794 a         1041 c          904 g          716 t
ORIGIN      
        1 tgctgtaaac atgtccaggt ttgttagacc aagactggct tcgatctctt ccattcgctt
       61 caacgcagtc tgaaaaccat cggcgccaaa gatctcggtc tcgatctcct caaacttctc
      121 gcccagctcg ctatattcct gttcgcccgt ggctgtcttc catgccggaa acacgactgt
      181 atcttcgacc gtcgcatggt gctcgtacat ccgcacaaac gactccaggg ccttggcaac
      241 actatcgacc tggcctgccg aaatccgatc gcccttcgaa acagacaaca ggtaatcagt
      301 aatctcacgc ccgcgggcgt gctgggccac cagcacgtcg gcaaacgccg ccaccggccc
      361 ttgtactttt ctgatcaacg gaatgacaaa actctcctcc agccgctttt cgtgatagtc
      421 ctcgccgaaa acgcgaaaca actgcgccgt tttctcgagc gcttctgccg gcatcgccgc
      481 cggatcctgc cgcaatctaa tcgcggactc ctgatagacc agcaacgcgc gccgtaaaat
      541 tccgtgttct cgcatcaagt cttccgtggc cgtcacttcg atcggcaccg cttcccctgg
      601 cgctggttct ctgccgtcgt cgtttgccgc gccgcgcctg ttggttgaca tcgtgccgca
      661 accaatcaac agcgcgcctg ctccggccag cgaaagagtg gttagcagtt gtcttctcgt
      721 ctcgttcact gtgctgctcc tttacggact ggtattacgg tgctcgcgag attagactag
      781 cacacactca aaagacccaa tcggggaatt catggacaac caggagatcg ctcgccggtt
      841 ctatcgcttg tccgcgctga tggagattcg cggtgacgat ccgtttcgcc tgcgctcgta
      901 tcgcaacgcc gccgaagcga tcgaagtctg gcccacgcca ctgaaagaga ttgctgagaa
      961 agaaggcctg gcgggcttac aggaaattcc aggcgtcggt aaggccatcg ccggcaaagt
     1021 gatcgatctt ctcgagcggg gaacgttcga cgcctgggaa cggcttacag ccgaaacacc
     1081 cgaaaccgtc ctcgatctca cggagattcc cgggattgga ccaaaaacgg ccgcgctgct
     1141 gcatcaacgt ttcaaagtct cttcattgcc ggagttgaaa gcttttgttg ccggtggcgg
     1201 gctggagatg gttgacggta tcggtccaaa aaccgaagaa agaattaaag aagctctgga
     1261 tctctgagct ataatgcgcc gctatgacaa agaagataaa acgccgtgac ttcatgcgca
     1321 ccggcgccgc tgccggtcta accatcgctg ccaccagcaa acaggccgcg ggctttccgt
     1381 cgatcctgac gcaaagcacc gtcaaacctc tcgtgatctc gtcggccaac ggcaatcgat
     1441 tcaaggatgg cggcactatg accgccgtgc agaaggcgtt cacgatgatg acgagcggtt
     1501 cagacgtgct cgatgccctc atcgcgggtg tcaacatcgt cgaactcgat ccactcgacg
     1561 acagcgttgg ttacggcggt ttgccaaacg ctgaaggcat cgtccaactg gactcgtccg
     1621 tcatgcatgg tccaaagaaa cgcgcgggcg cggtcggcgc actcgaaggc gtacgcactc
     1681 cttcgctcgt cgccagagca gtgatggaga acactgacca tcacctgatc gtcggcaaag
     1741 gcgctcagga cttcgcgcgt aacatgggct tcaagatcga agaagatctc aacactgaga
     1801 actcccgcag gcggtggctc gagtggaaac gccggacaga tcccagccac tatctcgatc
     1861 cgaaaaaacg cgccgaagtt ggtcatcgca tcgcgctcga gatgctcgcg gaaggaaagc
     1921 tgcgcgaaga gaacctgcac ggcactatca actgcgacgg catcaactcg aagggcgaaa
     1981 tctgcggcgt gacgaccacc agcggactcg catggaaaat tcccggacga ctcggcgact
     2041 cgccgattct cggcgccgga ctttacgttg acggcgcgat cggcgccgcc ggttcaaccg
     2101 gacgtggcga agcaaacctg ttcaatctgt gttcgtttgt cattgtcgag gagatgcgtc
     2161 gcggcgcgca ccccaaagac gcgggcatgg aagtgctgaa gcgaatcaag aacaacacga
     2221 tagagaaacg actgctgaac agccgcggcc tgccgaactt cggcatcagt ttctacatcc
     2281 tcaacgccaa aggagagtat gccggcgtga cgatgtacga gggcggatcg tttgcgatct
     2341 gtaacgacaa cggaccacaa acgttgaaag cggagccgct actccagggc aggccgagcg
     2401 attaagacca cggattacac cgaacccaca gacataaagc cgtgtaatcc gtggccgctt
     2461 ctacttcgcc gccgaatcgt tctgcatcat cccgaagata tttccttccg tatctttgca
     2521 gtagaccaac caaccaacac cggggattgc catctttggc aacgcgacgg tcgcgccgtt
     2581 gtcgagcgca gtctttaacg aagcatcaac atccgccacg tcaacggtgc agacgtacgc
     2641 gatgaccgcc tggccgtcga tctcgccctg acggcgcatc aggccgccgt tgatgccggg
     2701 ttgatcgtcc ggaccggtgt tgacgaccca gtactccatc ggtccttccc atttctgaaa
     2761 actccagcca aacagtttct ggtaaaaatt gacggcacgc tccggatcgc cggcgtgaat
     2821 ttcgaagtga acaactcttg gcatttatag tctccttcta tgaagtctgc ggaacagttc
     2881 cgtatttctg tgttgcaaaa cccactatca gcccgacgat tgtgcccggc acaatgatct
     2941 cgaaataata gccaccggtc atccacgcag tcagatacgc cagcagcgca cccactgcca
     3001 caccgaacag gatcccaatc ggcagcgatt gaaacttctt tgcaaaaaag ccaattgcga
     3061 ttcccgcgat gatgcctttg atcgtggagc cgatgacgat gccgccaagt tggtctctga
     3121 cggccggggt aaaccacgcg gtgagaccgt cgatgacgcc gagtatcgca ccgagtacta
     3181 ctccaagcag gggtttggac atttcggttc tccttcattt catctttgat tgccaattgc
     3241 cgattgccga tttggtgggc ttgcgagact caaaacacaa tcgcgaagca tcaatcaaaa
     3301 atcggcaatc ggcaatcggc aaggctttgc ctcaaaagtc ttctttcatt ctcaccgggc
     3361 ttcagcccgg tgttaccgcc acgcggtttt gagaaaccgt tttaacggtt tcgctggcga
     3421 atcaacggaa aaccgttgaa acggttcctc cgtct
//
LOCUS       DASTEY010000005         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_395606, whole genome shotgun sequence.
ACCESSION   DASTEY010000005 DASTEY010000000
VERSION     DASTEY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395606"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="VFO72_00085"
     CDS             complement(<1..1751)
                     /locus_tag="VFO72_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9477707.1"
                     /translation="MAQVLERSNNNLEKNIGLVRQQAVEVAESASQITSTANEVAEGA
                     EVQLRVLEKAVVIADEITASMGETTTQLESIATSTEELASSVNESTASIEEVTRNAES
                     LSASITQISAAVEQSARSIEGVSNTTQNMATSAEEVTGAMTEMASSVKAVSADTEALT
                     ASVNETAASIEEVAASIASLSSNADDLTVSAEETSSAINEMAASIEEVGAMTENLASA
                     VEEVSTSIEQMARSVQGVAQNAERITEAATTAAGSANQMDRSLHSVVALTKRAEEATG
                     QLSRQAEEGGAAAQRSMQGLMRVRSAMEQSAGVIREMGKRSDEIGGIVNTINVIAERT
                     NMLSLNASIEAARAGDAGRGFAVVAEEIRNLADRSAQATADIAQIIRSLQEVVREAVT
                     AASEGMRIADESGQLAEEGAAGLKKIMNGITQTAQLVGQIADATEEQLTAGQQVVAAI
                     DTTAMQTKQVSSAVVEQAQAAQQIVKSAIQMRKTAKEVSKAVNEQGSAAREIIKAAQS
                     TSGAAKQVRKASSEQAAAAQQITQSVESMRKRALSTARGLSEQAVAGDQVSKEAVQLA
                     RQIHEISRAIAEQAKASA"
     gene            complement(1714..2190)
                     /locus_tag="VFO72_00090"
     CDS             complement(1714..2190)
                     /locus_tag="VFO72_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9477708.1"
                     /translation="MEPFILFELAGTTYGVRSHFVQQIEMIENVASVPNAHAAVEGVV
                     IVRGQVIPALNLRTRFGFDKVERDLRSRLVVINAGARVVGLIVDTAREFLKIPAESIE
                     PPPEALTGLSGKYLDGVATIGERMILVLNLDAVLDLEYEDGRQDYGSGARALEQQP"
     gene            complement(2207..4138)
                     /locus_tag="VFO72_00095"
     CDS             complement(2207..4138)
                     /locus_tag="VFO72_00095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET9477709.1"
                     /translation="MDKPNPDNRGDFVDQFLDDYYAECDEHLVSIRRSVVTLEDQANA
                     HSVDYTLLDNLFRSFHTLKGISGMVGLAQAEQLAHHLESYLRELRDGTVTLNASGFEV
                     LAAGVSLLQNVINARRNDQTVPSIDAITDRLQAVSSESTASDLEQTSVSQNAAKTSSK
                     RWQVEFTPNLELAERGVNVNSVRVRLQELGELLQAKPIVKGAGEIAFQFVVATNAERT
                     MLNNLEADGLTFVALPDETAPKIVESQPVPTIAPASVVRVDLDRLDELMRIIGELVIS
                     RTRLEDQLAELKRVTPAAAWRSLQETSLSMGRQLRDLRESVMRVRMVQIGEIFERMTF
                     VVRDLARESGKKILVQLSGGETEIDKFLVERMMDPFMHLVRNAVSHGLETVAEREAQG
                     KRAEGLLSLSATTAGERIVIKIADDGRGIDRNLVIASANARGLNQGNADIDDGALLDL
                     ICTPGLSTRHEADRESGRGVGMDVVKRAVEELGGRISLSTKIGEGTTFTIELPLTLAI
                     AEALIVSVNGQRFAVPQSAVREVLEVESASTRVLENNEIISYRGRVLPLLRLARVFDM
                     NYQRGASFHVLVIGEESNAVGLAVDRILGQREIVVRAIRDPLAQSKGIAGATELGDQR
                     VVLILDITALRQLAVTSRH"
     gene            complement(4131..4538)
                     /locus_tag="VFO72_00100"
     CDS             complement(4131..4538)
                     /locus_tag="VFO72_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9477710.1"
                     /translation="MSSEVSSKVKTNNVLVVDDSPTMRRMVITSLGCLQRVKSTQAGS
                     GLEAIERLAIEPVDLMVLDLNMPDMHGMEVIDFVRSHQAYRDIPIVVLTTKTEEASRS
                     EALAAGADRYLTKPFDPWQLADVVGSLLKTSNG"
BASE COUNT          991 a         1464 c         1299 g         1208 t
ORIGIN      
        1 gccgaggcct ttgcctgctc agcaatcgcg cgactgatct catgaatctg acgggcgagt
       61 tggaccgctt ccttcgaaac ctgatcgcca gcaactgcct gctcactcag cccacgcgca
      121 gtcgagagcg ccctcttgcg catcgattcc accgactgcg tgatctgttg ggcggccgca
      181 gcttgttcgc tcgaagcttt gcgcacttgc ttcgcagcac cactcgtgct ctgcgccgcc
      241 ttgatgatct cccttgcagc gctgccttgc tcatttacag ctttgctcac ttcctttgcc
      301 gtcttccgca tttgtatagc agacttgact atttgctgcg cggcttgagc ttgctctacc
      361 actgcactcg aaacctgttt cgtttgcatg gccgtcgtgt cgatagcggc aacgacttgt
      421 tggcctgcag tcagttgttc ctctgttgcg tcggcgattt gtccaacaag ttgggcagtc
      481 tgagtaattc cattcattat cttcttcagg ccagccgcac cttcctctgc aagttgtccg
      541 ctttcgtcgg ctatgcgcat tccctcactc gcggcggtca ccgcttctct gaccacttcc
      601 tgaagcgatc taatgatctg cgctatgtcg gcagtcgcct gagcagatct atccgcaaga
      661 tttctgatct cctcggcaac cacggcaaaa ccacggccgg catcgccggc gcgagcagcc
      721 tcgattgaag cgttcagcga aagcatgttg gtgcgctcag caatgacatt gatggtgttt
      781 acgatgccgc cgatctcatc agaacgtttt cccatctcgc ggatcacgcc cgctgattgt
      841 tccatcgccg aacgcacgcg catcaatccc tgcattgatc tctgagcagc ggctccgcct
      901 tcttccgctt gtctgctcag ttggccagtg gcctcttcgg cgcgcttggt gagcgcaacg
      961 accgaatgta acgagcggtc catctgatta gcactaccgg cagcggtcgt cgccgcttca
     1021 gtaattcgct ccgcattctg ggccacgcct tgtaccgagc gcgccatctg ttcgatagaa
     1081 gtcgacactt cctcgactgc cgatgcgagg ttctccgtca tcgccccgac ctcttcaatg
     1141 gacgcggcca tttcgttgat cgcagaagac gtttcctccg ctgaaacggt taagtcgtcg
     1201 gcattgctgg acagactggc tatcgacgct gccacttctt ctatgctggc cgcagtttca
     1261 tttacggatg cggtaagcgc ctctgtatct gcactgacgg ctttgactga cgaggccatc
     1321 tcggtcatcg cgcccgtaac ttcctcggcg gatgtagcca tgttttgggt ggtgttgctg
     1381 actccctcga tcgaacgcgc agactgctcg acagcagcgg agatctgcgt gatggaggct
     1441 gaaagagatt cagcatttct ggtgacctct tcgatcgatg cggtgctctc gttaactgac
     1501 gacgcgagtt cttcggttga ggtagcaatc gactcgagtt gcgtcgtggt ctcgcccatg
     1561 gaagcagtta tctcgtcggc gataacgacc gctttttcga gcacgcgtaa ctgaacttcc
     1621 gctccttcag ccacctcgtt ggccgtcgac gtgatttgac tggccgattc ggcaacctcc
     1681 accgcttgtt ggcgcaccaa tccgatgttc ttctcaaggt tgttgttcga gcgctctagc
     1741 acctgagcca taatcctgtc tcccgtcttc atattccaga tcaagcactg cgtctaagtt
     1801 caataccaaa atcatgcgtt cgccgatcgt ggcgaccccg tcgaggtatt tcccactaag
     1861 cccggtgagc gcctcgggtg gaggctcgat cgactccgcc ggaattttga gaaactcgcg
     1921 ggccgtgtcg acgatcaatc cgaccacgcg ggcaccggcg ttaataacaa ccaatcgaga
     1981 acgcagatcg cgttcaactt tgtcgaaccc aaaacgtgtc cttagattca gcgccggaat
     2041 cacctgacca cgaacaatga cgactccttc taccgcggca tgagcatttg gcactgaggc
     2101 aacgttctcg atcatctcga tctgttgcac aaagtgactg cgaacgccgt acgtcgtgcc
     2161 ggccagttcg aaaagtatga atggttccat ctgtgtcatc cgtcgtttag tgccgcgacg
     2221 ttactgccaa ctgccgcaat gcagtgatgt ccaaaatcag caccactcgt tgatctccga
     2281 gttcggtagc tccggcgatg cctttactct gcgcaagtgg atccctgatg gcgcgcacca
     2341 cgatctctcg ttggccgagt attcgatcga ccgccagacc gacggcgttt gattcctcgc
     2401 ctattaccaa cacatgaaag ctcgcaccgc gctggtaatt catgtcaaag acacgagcga
     2461 gccgaagcaa aggaagaact ctgccgcgat acgaaatgat ctcgttgttc tccaacaccc
     2521 tggttgaggc tgactcgact tccagcactt cccgcaccgc cgactgtggc acggcaaacc
     2581 tctgcccgtt cacactgacg atcaacgctt cagcgatcgc gagtgtcaga ggcaactcga
     2641 tcgtgaaagt ggtgccttca cctatcttcg tcgacagcga gatccggccg ccgagttctt
     2701 cgacagccct cttgactacg tccattccca caccgcgtcc actctctcga tcggcttcgt
     2761 ggcgtgttga taatccggga gtgcaaatca gatccaataa cgcgccgtcg tcgatatctg
     2821 cattcccctg gttcagaccg cgagcatttg cactggcaat gacgagattt cgatcgatac
     2881 cgcgcccgtc gtcggcgatt ttgataacga tcctttcgcc ggcagtagtc gcgctgagag
     2941 ataacaaacc ttcagcccgc ttcccttgtg cttcccgctc ggcaacggtt tcgagaccgt
     3001 gacttacggc attgcgtacg aggtgcatga agggatccat catccgttcc accagaaact
     3061 tgtcgatctc cgtttcgccg ccgctgagtt gcacgaggat cttctttcca ctttcacgcg
     3121 ccaggtcgcg aacgacgaaa gtcatgcgct caaagatttc gccgatctgg accatgcgca
     3181 cgcgcatgac actctcgcgc aagtctctca attgccgtcc catgctcaga cttgtctcct
     3241 gaagcgaacg ccaggccgcg gcgggcgtca ctcgtttcaa ctccgccagt tgatcttcga
     3301 gccgcgtgcg gctgatcacg agttcaccaa ttatgcgcat cagctcatcg agccggtcga
     3361 gatcgacgcg aacaacgctc gccggagcga tagtcggaac gggctgactc tctacaattt
     3421 ttggggcggt ttcgtctggc agtgctacga aagtcaaccc atccgcttcg aggttattaa
     3481 gcatcgtccg ttcggcgttc gtggcgacga cgaattgaaa cgcgatctct ccggcgcctt
     3541 tgacgatggg tttcgcctgt aacaactcac cgagctcttg cagtcgcaca cgcacgctgt
     3601 ttacgttcac gcctcgctcg gcaagttcca aattaggtgt aaactcgacc tgccaacgtt
     3661 tggaacttgt cttcgccgcg ttttgcgaaa ctgatgtttg ctcaaggtca ctagcggtcg
     3721 attcagaaga gaccgcttgc aagcgatctg taatcgcgtc gatagaggga acggtttgat
     3781 cgttgcgacg agcgttgatt acgttttgga gtaaagaaac tcccgctgcc agcacttcaa
     3841 acccggatgc gtttagcgtc acagttccat cacgcagttc gcggaggtaa ctttcgagat
     3901 ggtgggcgag ttgttcggct tgggccaggc caaccatgcc ggagattccc ttgagagtgt
     3961 gaaaactgcg aaacagatta tcgagtaaag tgtaatcgac tgaatgtgcg tttgcctggt
     4021 cttccagcgt aaccacggag cggcggatgg agaccaggtg ttcgtcacac tcggcgtaat
     4081 aatcgtcgag gaactggtcg acaaagtcac cacggttgtc agggtttggc ttatccatta
     4141 ctcgtcttta atagtgagcc cacgacgtcg gccaactgcc agggatcaaa aggtttcgtc
     4201 aggtagcgat ccgcgcctgc cgcgagagct tctgaacggc tggcttcctc ggttttagtc
     4261 gtcagaacga cgatcgggat atcacgatag gcctggtgac ttcggacgaa atcgatcact
     4321 tccatgccgt gcatgtccgg catgttgagg tcaaggacca tcagatctac aggctcgatc
     4381 gcgaggcgtt cgatggcctc caggccgctg cccgcctgtg tagacttaac cctttgcagg
     4441 cagccgagcg aagtaatgac catacgtcgc atggtgggcg aatcgtcaac gactagaaca
     4501 ttgtttgtct tgaccttgct tgacacttca gaagacacac ttcagttgct agattggccg
     4561 gagcagggga aacgcgtggc aaatcagggt ctgtgtaaat tgggggacgc ctattgtacg
     4621 ggaaggtaag gaattacgaa agcgagactt actaaacgtc ccgcggtctc ctttacccgt
     4681 taattaagga cgttctggtt gttcgggccg cagcccaagt aatgcacatt cgggttctca
     4741 atgagcggcc tcttgcttac tccaccggta gtgatgcagt ttgaaactta ggcactttcc
     4801 tgcggggacc gcagccgtct cgcctgcacg gtgacgaatt ggtcgctgcc gtgagatgca
     4861 ggcgaaacgc ctgcggtccc agagtagacg gttcgtagct tcaaactgca tccactactt
     4921 actccccttt tcggtcgcat tcgctggagc ctgtgtgctg gc
//
LOCUS       DASTEY010000006         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1466830, whole genome shotgun sequence.
ACCESSION   DASTEY010000006 DASTEY010000000
VERSION     DASTEY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466830"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFO72_00105"
     CDS             <1..719
                     /locus_tag="VFO72_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477711.1"
                     /translation="AMFWILTPDPHRGYGVTYATSRDEGVLTEVSRASQMFAALQSAE
                     LPAHLQDTSRHLIPRQFAWKRATADPATMPEVIFREDRSVLYQFKPQMASAQRFEKIG
                     EGPGYIWGFGSGFLEYTVPAREDRRRVSEIIVRAHIQPVLPIDAPDGFVKTRATLFVE
                     GWNGGSRLIGVEPKDQPVIQEWRLTGRLLRLRAMRGLPLTIRFAVTPDSDWLYGVNIS
                     NWPEGYDSRNARPVEVELRR"
     gene            complement(787..2475)
                     /locus_tag="VFO72_00110"
     CDS             complement(787..2475)
                     /locus_tag="VFO72_00110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9477712.1"
                     /translation="MSARAEAIVTAGLRPELFAGRGLLANSRALVFAALAVLILAGFG
                     FRVTGLSAEGLSEDELNKLNAVAEYRAHGLTGANGEHPMLMKALQTASIVVAEKWNSL
                     ARASDQIAPETALRLPGAIAGALTVLLIYLIGAELFGAEVALIAAALWAFDPAAIAFN
                     RVAKEDTFLLFFFLLANVFWLRGQRVAESTDQNPNKYYWATAACYGAMVASKYLPHLF
                     AISMCYYWMFQQLPETRWRLGKKRILTLFAIMFLVFLVLSPTVLLPETWRQMGLFAGG
                     KRVSHDGYEFMNQLYTQRFSDWLNGIPVYFYLVFTAVKLPVLTVLGFLAGLPLLFRRK
                     LGDGRYFILFWLFLWVVAFCFPGGKFTRYYTTVLPAVLITCALGIQFTGRWLAKRIAS
                     IDIAAGLKHYVPACLAVVLIIGSVMNSVQASPHFRLYTNAFGGGTQWAGYYFPHDEFY
                     DASMRDVISDVAARAKPGARVASESPTLAAYYAVRAQRTDLICVSLSDPEALKQLTAD
                     DFVIIARGRRYFSNEAITNALRDHSSPVAEFKLGPAASAKLYQLDQHSLSLLAP"
BASE COUNT          634 a          741 c          782 g          522 t
ORIGIN      
        1 gcgcgatgtt ctggatactg acgcccgatc cgcatcgcgg ctacggcgta acgtatgcga
       61 cgtcgcgtga cgaaggcgtg ctgactgaag tgtcacgagc ttcgcagatg ttcgcagcgt
      121 tgcagtcggc ggagttgccg gcgcatttgc aagacacgtc acgtcatttg attccgcggc
      181 agtttgcgtg gaagcgtgcg acggcagatc cggcgacgat gccggaggtg atcttccgcg
      241 aagacaggtc ggtgctttat cagttcaagc cacagatggc gtcggcgcaa cgcttcgaga
      301 agattggtga gggaccagga tacatctggg gctttggctc gggatttctt gagtacacag
      361 ttccggcgcg tgaggatcgc cggcgcgtga gcgagataat agtgcgcgcg cacatacagc
      421 cggtgcttcc gatcgatgcg ccggacgggt ttgtaaagac gcgcgcgacg ctgtttgttg
      481 aaggttggaa cggcggctcg cgtttgattg gggtggaacc aaaagatcag ccagtgatac
      541 aggagtggcg cttgactgga aggttgctac gcttgcgagc gatgcgtggg ttaccgttaa
      601 cgattcgctt cgccgtgaca cccgactccg actggctcta cggcgtcaac atctctaact
      661 ggccggaggg ttacgactct cgaaacgcca gaccggtgga agtcgagcta cggcgttagg
      721 tccttcatcc acagattaca cagattattt aatcaatctg aataatctgt ggatagggaa
      781 gcgaaactat ggtgccagga gactgagcga gtgttggtcg agttggtaga gtttggcgga
      841 agcggcgggt cctagtttga attcggcgac cggcgaggag tggtcgcgca gggcgttggt
      901 tatggcttcg ttgctgaaat agcgacggcc gcgagcgatg attacgaagt catcagcggt
      961 tagctgcttt agcgcctccg gatcggagag cgagacgcag atcagatcgg tgcgttgggc
     1021 gcgcaccgca tagtacgccg ccagcgttgg gctttcgctc gccacacgcg ctccgggctt
     1081 cgcgcgcgcc gcaacgtctg aaatcacgtc gcgcatactt gcatcgtaaa actcgtcgtg
     1141 cggaaagtag tagccagccc actgcgtgcc cccgccaaag gcgtttgtgt aaagacgaaa
     1201 gtgcggagaa gcctggacgg aattcatcac tgaaccgatg atcaacacga ccgctaaaca
     1261 cgcaggcacg tagtgtttca aacccgccgc aatgtcaatc gacgcaattc gcttcgccag
     1321 ccagcggccc gtgaactgaa ttcccaacgc gcatgtgatc agcactgcgg gtaagaccgt
     1381 cgtgtaatat cgcgtgaatt tgccacccgg gaagcagaac gctactaccc acaggaacaa
     1441 ccagaaaaga atgaagtagc gcccatcgcc gagcttgcgc cgaaatagca gcggcaggcc
     1501 ggcgagaaaa ccaagcacag tcaacaccgg cagcttcacc gccgtaaaca caagatagaa
     1561 gtaaacaggt atcccattca gccaatcact aaagcgctgc gtgtagagct ggttcatgaa
     1621 ctcgtaaccg tcgtgactta ctcgcttgcc accggcgaac agccccatct gtctccacgt
     1681 ttcaggcagc agaaccgtcg gactcaaaac cagaaacacc agaaacatga tcgcgaataa
     1741 cgtcagtatt cgctttttgc ccagccgcca acgcgtttcc ggcagttgct gaaacatcca
     1801 gtagtaacac atgctgattg caaacaagtg cggcaggtat ttcgacgcca ccatcgcacc
     1861 gtaacacgca gcggtggccc agtagtactt gttcggattc tgatccgtgc tttcagcgac
     1921 gcgctgcccg cgcaaccaaa acacatttgc gagtagaaag aagaagagca ggaacgtgtc
     1981 ttccttagcg acacgattga aagcaatcgc ggccgggtcg aatgcccaca gtgcagccgc
     2041 aatcaaagcc acttccgcgc caaacagttc agcgccgatc aagtagatca agagcaccgt
     2101 gagtgcaccg gcaatcgcgc cgggcaaacg cagcgcagtt tcgggagcta tctgatcaga
     2161 agcgcgggca agcgagttcc atttttcagc gacgacgatc gacgcggttt gcagcgcctt
     2221 catcagcatc ggatgctcgc cattggcgcc ggttagacca tgcgcgcggt actcggcgac
     2281 agcattcagt ttgttcagct cgtcttcgct aagaccttcg gcgctgagtc ctgtcacacg
     2341 aaaaccgaag cccgccagaa tcagcactgc aagtgcggca aagacaagtg cgcggctatt
     2401 agcgagaagg ccgcgtccgg caaataactc tggccttaga ccagcggtga cgatcgcttc
     2461 agctcgtgcg gacaaaaata gctcccttca acaaaatgcg aaaggttagc gaaaaccgtc
     2521 gctaagtgtc aaaaccaaat ccacagattg gttagtgatg tagtctgaat ccggtgccga
     2581 ttcccggacg cgctcttccc tgggaccgca ggcgtctcgc ctgcacggag acgaatcggg
     2641 cgcttctcgt gacatgcagg cgagacgcct gcggtccca
//
LOCUS       DASTEY010000007         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_478013, whole genome shotgun sequence.
ACCESSION   DASTEY010000007 DASTEY010000000
VERSION     DASTEY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478013"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2863
                     /locus_tag="VFO72_00115"
     CDS             <1..2863
                     /locus_tag="VFO72_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET9477713.1"
                     /translation="YTFPVSGAGSISIKTVVQQGDKHYTSLGGFIWVASERQEAFYYG
                     AYYNDAGIKLVPDKKSYRVGETAHVLALLPKEGANLLVTTELDTVMSARHVHVPGRTV
                     VLDVPIEANYSPNVYLNVTFVQNGDMFTGDQRLVVPARDKLMALEIISNKQEYKPREI
                     ASYTILARDADGAPVRDAEVSLGVVDEAIYSVSADYSGNIRQRFYGTRYNSVQTHLSI
                     AYYFRGFAGEKPLNLASTKRSYQLADFKNEGEQVQPMIRKEFKDTAYWQPSAVTGNDG
                     RATVKFRLPDNLTTWRATARAVTSDTKVGVAKSKVISRKDVILRLETPRFLTQGDTVT
                     LSGIVHNYLKQPKSTQISIAVNGAQLIGPPQKTVTINQQGEHRVDWQISAPQTGQITL
                     LAKALTNTESDAVELTLDVVPRGLHEIKAERWMTTDDKAEQQFSLEVPANADLNSRRL
                     RVEVAPSIAGTLFGGLDYLTTYPYGCTEQTMSSFLPNIIVSQTLKEFRTASIRTNNDL
                     KRKVERGRDRLYAFQVQDGGWGWWKADQSDPFLTAYVFNGLTLAKQAGYDIDDERLAR
                     ARERLQSFLDSDDRKTDSSTRAFMVYALAESGGVDNRHVEKLFAERHSLQPYGRAMLA
                     LALSAQKMNQRALEVAGEIEQTAQVSKASAYWQSKVTGYYGFTDKHLTEGTALSLKAL
                     ARIKPQSSLLPLAARWLVFERKNGYYWNSTRDTAFAIHGLIDYVKVSRELTPSYDLEV
                     YVNGETVVAERVSDTSAARTFLLNRKGSAVGATNHIRVVKRGKGALYFSSSVEYYTDD
                     ANVAARGSSELNVTREYYRLKVEEDGHKLEWSISPLTGEIRSGDLLVVKLHVTGQPAR
                     HLMLEDPIPSGGEQVEAVGNLNLTYSDQRWTDWFSSREFRDRRTVYFLDYFDGDTTFQ
                     YALRVQVPGEFVVAPGRVELMYEPWKNANTATHRFTFLN"
BASE COUNT          687 a          816 c          890 g          617 t
ORIGIN      
        1 ctacaccttc cctgtctcgg gcgcgggcag catctcgatc aaaacggtgg tccaacaagg
       61 cgacaaacat tacacctcgc tgggcggctt tatttgggtg gcaagcgaac ggcaggaggc
      121 gttttattac ggcgcctact acaacgacgc aggtatcaag ctggtgccgg acaagaagtc
      181 atatcgcgtg ggcgagacgg cacacgtcct ggcgttgttg cccaaagagg gcgccaacct
      241 cctggtaact accgaactcg atacggtcat gtctgcgcgg cacgtccatg ttcccggtcg
      301 cacagtcgtt ctcgatgtgc caattgaagc gaactattcg cccaacgttt atctcaacgt
      361 cacttttgtt cagaacggcg acatgtttac gggcgaccag cggctcgtcg tgccggcacg
      421 cgacaagctg atggctctcg aaatcatctc gaacaaacag gagtacaagc cgcgcgagat
      481 cgcgtcgtac acgatcctcg cgcgcgacgc tgatggcgcg ccggtccgcg acgcggaagt
      541 gagtctgggt gtggttgacg aagcgatcta cagcgtgtca gcagattact caggcaatat
      601 tcgccagcgc ttttacggca cgcgctacaa ctcagttcag acgcatctct cgatcgctta
      661 ctacttcagg ggatttgccg gcgagaagcc gttgaacctg gcgagcacga aacgttcgta
      721 ccagttggcc gacttcaaga acgaaggcga gcaagtacag ccgatgattc gaaaggagtt
      781 caaagacacg gcgtactggc agccgtccgc tgttaccggc aacgacggcc gggcgacagt
      841 gaagtttcgt ctgcccgaca acctgacgac gtggcgcgcc acggcgcgag cggtgacgag
      901 cgatacgaaa gtgggcgtcg caaagtcgaa agtgatctcg cgcaaggacg tgatcctgcg
      961 gctcgagacc ccgcgcttcc ttacccaggg cgacaccgtc acgctctccg gcatcgtgca
     1021 caactatctg aaacaaccaa agtcaacgca gatttcgatc gccgtgaacg gcgcccagct
     1081 aattggcccg ccacagaaga ctgtcacgat caatcaacaa ggcgaacacc gcgtcgattg
     1141 gcaaatctca gcgccgcaga caggacagat cacgttgctg gctaaagcgt tgaccaacac
     1201 ggagtctgac gcggtggagt tgacgctgga cgtcgtgccg cgtggacttc acgagataaa
     1261 agcggagaga tggatgacga cggacgacaa ggccgagcaa cagttttcgc ttgaggttcc
     1321 ggccaatgca gatttgaact cgcgacggct gcgggttgaa gttgcgcctt caatcgccgg
     1381 cactttgttc ggcgggctcg actatctgac gacgtatccg tacggctgca ccgaacaaac
     1441 gatgtcgagt tttctgccca acatcatcgt ttcgcagacg ttaaaggagt tccgcacagc
     1501 atcgattcgc accaacaatg atttgaagcg caaagtcgaa cgcgggcgag atcgtctgta
     1561 tgcgtttcaa gttcaggatg gcggctgggg ttggtggaaa gccgatcaga gcgatccgtt
     1621 tctgaccgcg tacgtcttta acggactcac gttggcgaaa caggccggct acgatatcga
     1681 cgacgaacgc ctcgcgcgag cacgtgaaag attacaaagc ttcctcgaca gcgacgatcg
     1741 caagactgac agcagcacac gcgcattcat ggtctatgca ctcgcggaga gtggtggggt
     1801 cgacaatcgt cacgtcgaga agttgttcgc tgaacgtcac agcctgcaac cgtacggccg
     1861 cgccatgctc gctctcgcgt tgtcggcgca gaagatgaat caacgggcgc tggaagtggc
     1921 gggagagatc gagcagacgg cacaggtaag caaggcgtct gcttactggc aatcgaaagt
     1981 aacaggttac tacggtttca ccgacaagca cctaacggaa ggaactgcgc tgtcgttgaa
     2041 agcgctggcg cggatcaagc cgcagagttc gttacttccc ctcgcggcgc gctggctagt
     2101 gttcgaaaga aagaatggtt actactggaa ctccaccagg gacacagcct tcgccatcca
     2161 cggcctgatc gactacgtca aagtcagtcg cgaactcacg ccttcgtacg atctcgaggt
     2221 gtacgtgaac ggcgagacag tcgtcgccga acgcgtgagt gacacgagcg ccgcgcgaac
     2281 gtttctcttg aatcgcaaag gctcggctgt aggcgcgacg aatcacattc gcgtcgtcaa
     2341 acgcggcaag ggcgctctct atttctccag ttccgttgag tattacactg acgatgcaaa
     2401 cgtcgcagcg cgtggttcgt ctgaattgaa tgtgacgcgc gaatactacc ggctcaaagt
     2461 ggaagaagat ggtcataagt tggagtggtc gatctcgcct ttgactggag agattcgttc
     2521 gggcgatctc ctagttgtga agttacacgt cactggccag cccgctcgtc acctgatgct
     2581 ggaggatccg attccgtcgg gcggggagca ggtcgaggcc gtcggcaatc taaatctcac
     2641 ttacagcgac cagaggtgga ccgactggtt cagctcgcgg gagtttcgcg atcgacggac
     2701 ggtctacttt ctcgattatt tcgatggcga tacgactttc cagtacgcgt tgcgagtgca
     2761 ggtacccgga gagttcgtag tggcgcccgg gcgtgttgag ttgatgtacg agccgtggaa
     2821 gaacgccaac accgcgactc atcggtttac gtttcttaac tgagtggagg ttacgaagat
     2881 gtttcttcag gcgatgaaag cgcagttgac agcgatacga ctgttgttca gaagcaagtt
     2941 caccttagtg ttactggtgt tgatttatgg tgggctgttg gctgcgggtt acctttttgt
     3001 gagcacgcgc
//
LOCUS       DASTEY010000008         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_692278, whole genome shotgun sequence.
ACCESSION   DASTEY010000008 DASTEY010000000
VERSION     DASTEY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692278"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFO72_00120"
     CDS             <1..749
                     /locus_tag="VFO72_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477714.1"
                     /translation="GSDNRYMDYWRALLQGDMGLSFRNDRPVSELILERYPATIELAI
                     VAMLIAVLIAIPLGVIAGTNRGTLIDNVASVVALLGISLPSFVIGPMLVYIFAVKMRW
                     LAPSGRFDWSDIILPAFTLGAALSAILTRMVRSSVIEELGEDYVRTARAKGLNERTVI
                     YKHVLKNGLIPVVTILGLQLGVLLAGAIITEKIFGWPGLGLLLVEDGIGKRDYRVVQG
                     CVLVISMTYIIANLLTDMVYRWLDPRIRVS"
     gene            750..1565
                     /locus_tag="VFO72_00125"
     CDS             750..1565
                     /locus_tag="VFO72_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477715.1"
                     /translation="MNRLTIIGLVIVVVLVIVALFAPWIAPYDVGATNLSMRYLPPST
                     EHWFGTDSTGRDIFSRVVFGARISLQVGIIVVTVSAIVGTIFGAIAGYYGGWVDRFVS
                     GYVFNVFLAFPGLLLAIAMVAFLGAGLNKLILALCIIGWVGYARLIRGQVLKVREYDF
                     VQAARALGASDARILLVHILPNAIQPLIVQASLGMAGAVLSEAALSFLGLGVPPPAPS
                     WGVMIEEARDLSTLQVAPHALIFPGTAIALTVLAFNFIGDGLREHLDPRQRAR"
     gene            1571..>2165
                     /locus_tag="VFO72_00130"
     CDS             1571..>2165
                     /locus_tag="VFO72_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9477716.1"
                     /translation="MSESAISIRNLSKTYPSPFRRLRAFFRRPVKEPVEALRDVTFDV
                     HSGEIFGLIGRNGAGKTTLTKIVATLVQPTTGSVSVRGNDSVLQDEHVRREIGLATAE
                     ERSFYWRLTAEQNLMFFARLHGLSDRTAKQRIRELFAKLELEELARRRFGEMSTGNKQ
                     RLAVARAMLSNPPVLLLDEPTRSLDPLAAARMRELIRS"
BASE COUNT          379 a          615 c          641 g          530 t
ORIGIN      
        1 acggttctga caaccgctat atggactact ggcgcgcgct cttgcagggc gacatggggc
       61 tgtcgtttcg caacgatcgt ccggtgagtg aactcattct cgaacgttat ccagcgacga
      121 tcgaactcgc catcgtcgcg atgctcatcg ctgttctgat cgcgatacca ctcggcgtaa
      181 ttgccggtac gaatcgcggc acattaatcg acaacgtcgc ctcggttgtg gcgctacttg
      241 gcattagtct gccgagtttt gtgatcgggc cgatgctggt ttacatcttt gccgtaaaaa
      301 tgcgctggct cgcgccgtcg ggacgttttg actggagcga catcattctg cccgcgttca
      361 cgcttggcgc cgcgctttcg gcgatcctga cgcgcatggt gcggtcgtcg gtgattgaag
      421 agctgggaga ggattatgtt cgcactgcgc gggccaaagg tttgaacgag cgaacagtga
      481 tttacaaaca cgttctgaag aacgggttaa ttcccgtggt gacgattttg ggtttgcaac
      541 tcggcgtcct gctggcgggc gccatcatca ccgaaaagat cttcggctgg cccggtttgg
      601 gtttgctgct cgtggaagat gggatcggaa aacgcgatta ccgcgtcgtg caaggctgtg
      661 tactcgtgat cagcatgacc tacatcatcg cgaacctgct cacggacatg gtctatcgct
      721 ggctagatcc gcggattagg gtctcgtaaa tgaaccggct gacaattatt ggactcgtca
      781 tcgtcgtggt gctcgtaatc gtggcgctgt ttgcgccgtg gattgcaccc tacgacgtcg
      841 gcgcaaccaa cttgtcgatg cgctatctgc caccttcgac ggagcactgg ttcggcaccg
      901 attcgaccgg ccgcgacatc ttctcgcgcg tggttttcgg cgcgcgcatt tcgttacagg
      961 tcggaattat cgtggttact gtgtccgcca ttgtcggcac gattttcggc gcgatcgccg
     1021 gttactacgg cgggtgggtc gatcgcttcg tttccggata cgtcttcaac gtctttctcg
     1081 cgtttcccgg tctgttgctg gcgattgcga tggtcgcttt cctcggcgcc ggacttaaca
     1141 aactaatcct cgcgttgtgc atcatcggct gggtcggcta cgcaagactt attcgcggcc
     1201 aggtgttgaa agttcgcgag tacgattttg tgcaggcagc ccgcgcgctg ggcgcaagcg
     1261 atgcccgcat tctgctcgtt cacattttgc cgaacgcgat tcagccgttg atcgtgcagg
     1321 cgagtttggg catggcgggt gcggtcctat cggaagcagc gctttcgttt ctgggtctcg
     1381 gcgtgccgcc acccgcgccg tcgtggggcg tcatgattga agaagcacgc gatctttcaa
     1441 cgcttcaggt cgcgccgcat gcgctgatct ttccgggcac tgccattgca cttaccgtgc
     1501 ttgcgttcaa cttcatcggc gacggcttgc gcgagcatct cgatccgcga caacgtgccc
     1561 gttgattgcc gtgtctgaat ccgctatctc cattcgcaat ctgtccaaga cttatccttc
     1621 cccgtttcgc cggttgcgtg cgttctttcg ccggccagtc aaagaaccgg tcgaagcact
     1681 tcgcgatgtt acgtttgacg ttcacagcgg cgaaatcttc ggtttgattg gccgcaacgg
     1741 cgcgggtaag acgacactaa cgaagatcgt cgccacgctc gttcaaccga caactggcag
     1801 cgtttccgtg cgcggcaacg acagcgtgct gcaagacgag cacgtacggc gggagattgg
     1861 ccttgccacg gcggaagagc gcagcttcta ctggcgtttg actgccgaac agaacctgat
     1921 gttcttcgct cgccttcacg gcctcagcga tcgcactgcc aaacagcgca ttcgagaact
     1981 gtttgcgaaa ttggaactgg aagagcttgc gcgtcgccgt ttcggcgaga tgtcgacggg
     2041 taacaaacaa cgactagctg tagcgcgggc gatgttgtcc aacccgcctg tcttgctgct
     2101 cgacgaaccg acgcgttcgt tggatccgct tgccgcggcg cgcatgcgcg agttgatccg
     2161 ttcgc
//
LOCUS       DASTEY010000009         6569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_263761, whole genome shotgun sequence.
ACCESSION   DASTEY010000009 DASTEY010000000
VERSION     DASTEY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263761"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFO72_00135"
     CDS             complement(<1..327)
                     /locus_tag="VFO72_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9477717.1"
                     /translation="MKALFGTDGIRGEAGRFPLDAATVTTIGFSLARHLAETVAEPVI
                     VTGRDTRESGEWLEQALIEGATQGGAKCSSAGVITTPGVAFLTRKLQANAGVVISASH
                     NPYQDNG"
     gene            complement(327..1283)
                     /locus_tag="VFO72_00140"
     CDS             complement(327..1283)
                     /locus_tag="VFO72_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET9477718.1"
                     /translation="MPFQDVDEVAEPVAPAPAPSRVEKLARKIFLEDWSLKLLSLAIA
                     LVLWLVVTGQNEPVTAHVNVQLNFIRPPSLEISNDPPRTVDVMLTGSRNKLDDLTSLD
                     LVATVDISDQRAGERVLRLADKAQIPLPQGVKVDGYQPSAIPLRLEPILERQVPIEPK
                     LEGQVAEGFEIYAVRPNKGSVTVRGPESRVVALQKVVTESILIGGQRESFTASNVAID
                     MPDPKVDLVDPVVTVNVEIGERRVERTFSGVAATAADGSKVQPSTGEVTLLGPASMVE
                     SLKPEELKIVVDGNGSRLELPPALKGKVSIKSILPGKFLLVK"
     gene            complement(1288..2133)
                     /gene="cdaA"
                     /locus_tag="VFO72_00145"
     CDS             complement(1288..2133)
                     /gene="cdaA"
                     /locus_tag="VFO72_00145"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET9477719.1"
                     /translation="MLLLLPYTEYLRRLVEPRNIADIVFVFLLVYAVLKLLRGTRAAP
                     MAAGIAALVFLYWLSIKQDLATLEFVLSNGLLYIGIAIIVLFQSEIRQALITFGNRFR
                     MPFSRHRGQFGEGVYDEVILAATTLASTKTGALIVIERNVGLKNVIDGGVQLDAELSY
                     DLLLSIFNTASPLHDGAVVVRRHRIAAASCFLPLTLNPRISRDLGTRHRAAIGVTEDT
                     DAVAVVVSEETGLLSFVQKGEIKRGLDATKLRHAIFEALGVLPRKEKELKKATDQDAE
                     EIAPV"
     gene            complement(2279..3433)
                     /locus_tag="VFO72_00150"
     CDS             complement(2279..3433)
                     /locus_tag="VFO72_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9477720.1"
                     /translation="MSFRNFYKRQITVARIFGIPVRFDYRWFVVFLLSVWLIAVNLRQ
                     GGMFVGSFRLQPVSTVVAWTLGVITTLGLFLSVLVHELSHAFMGRSEGIEIEEIVLHP
                     FGGLARLRHEPENPRAEFRIAVAGPAASFLIGAVTFVLLLPAVRSGYYIVAGVLLLLC
                     AGNLLLAVFNLFPGYPLDGGRVLRAILWRRTGDIRQATRMAGICGMLIAAILIIFGVY
                     MAIAPNFRSYFMGFWSVLVGVFLFDAAYSVVKHVQGRLQNIVREAMSAPFSIEPDLLI
                     SSLIDSVLPMHRQVAFPVARDRRLHGMLSLEDLKSLPRERWHLTRARDIMRPIAPRFF
                     VEPTARLDYARELMKRNGIGSLAVVGKNGELVGFLQSGKFRRKKRAKDSR"
     gene            complement(3436..4539)
                     /locus_tag="VFO72_00155"
     CDS             complement(3436..4539)
                     /locus_tag="VFO72_00155"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9477721.1"
                     /translation="MSFIGKTGHFLGLSALRKFVSDSMAIDMGSASTLIAVRGRGVVV
                     DEPSVVAVNKLTGEVIAFGNEAHKMQGREARDVTVIAPLVDGVVADFERTRLMLDHFV
                     RQARSGVSHFSRRAIMSVISEVTQVEQRALLAAAEEARISRVYMIEEGQAAAIGAGVP
                     LSDERAAAVVDIGGGTTNVAAIISGSIIASRAERIGSGDIDVAIMDRLRRHRGLTIGA
                     QTAERLKLELSSAIEPEDPNKKVNVRGRDVQTGSPAAVDITAGEVYTITMPIIRRIAD
                     EVRSMLTELAPEVAGDIYERGLILTGGGALLEGLSQYLQRELDLVVKVADDPRFAIVR
                     GLAQLYEEPLLLRRVARTEPHPLLDTEATAFET"
     gene            complement(4723..6390)
                     /locus_tag="VFO72_00160"
     CDS             complement(4723..6390)
                     /locus_tag="VFO72_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9477722.1"
                     /translation="MAHLKITDASGRQWQFSFVPQTACTIGRAPDNSVVLDDPRASRY
                     HAHINQAENGTFTIVDGAIINGQLRRSANKVFLNGEPRFEQQLKNGDRITIGASTLRF
                     EQPAAERTTDVRYDDKPLGHTQLLISANDVMSTVLRAKEDVPASAQRDKVLEALQRKA
                     NILSALYEMSKTLGSVFDLNAIFEKATDIIFRSTPADRVVALLAEPGDADGGDVNLTP
                     IAVRARDNKLEAHARRLSIGRTITRKVMKDRVALLSQDAASDEQFAGVDSIVSQGVRS
                     TICAPLMGESRVHGALYADRLDPFAAFKPDDLELISAVAAQTAIAVENARAHERLARE
                     EVARANYSRFLPEYVVQQMLLNPDSFKLGGVSQTITILFADIRGFTRISEHAPPEKIV
                     SLLNRYFSAMTDIIFAHGGTLDKYLGDGLMALFGAPTTTPDDASNALNAAVAMQRRLL
                     GINRELRDEGFPEIGVGMGMHTGEVIVGYIGSERRSEYTAIGDTVNTSSRLESNARGG
                     EILISDATAKAARSRYKLKPREPIMVKNRQQPVMLWEVDWQKASGSF"
BASE COUNT         1426 a         1916 c         1846 g         1381 t
ORIGIN      
        1 gccgttatcc tggtacggat tgtgtgaagc gctgattaca acgcccgcat tggcctgcaa
       61 cttacgcgtc aggaacgcga cgccgggagt ggtgattacg ccggcggaag aacacttggc
      121 gccgccttgt gtggcgcctt cgatcaacgc ctgctcgagc cactcgcccg attcgcgggt
      181 gtcgcgccca gtgacgatga ctggttctgc gacagtttct gcgagatggc gtgctaagga
      241 gaacccaatt gtcgtgactg tagctgcgtc gaggggaaaa cgtccggctt cgccgcgaat
      301 accgtcggtt ccaaaaagag ccttcattat ttcactagga gaaacttacc cggcagaatc
      361 gactttatac taacctttcc ctttaaagcg ggcggtaatt cgagtctcga accgtttccg
      421 tcgactacaa tcttcaactc ttcaggcttc aacgactcca ccatgcttgc tggtccaagc
      481 aacgtcacct cgccagtgga gggttgcacc ttactcccat cagcggcagt cgccgcaacg
      541 ccagaaaaag tcctttcaac gcgacgctct ccgatctcaa cattaacagt cacaaccgga
      601 tcgaccagat ccactttcgg atccggcatg tcgatcgcca cgttcgacgc cgtaaaactc
      661 tccctttgtc cgccaatcag gatgctttca gtaacgacct tttgcaacgc gaccacacga
      721 ctctcaggcc cgcgcactgt caccgatcct ttgttcggac gtaccgcata gatctcaaag
      781 ccttccgcga cttgcccttc cagcttcggt tcaataggga cctgccgttc aaggataggt
      841 tcaagtcgca gcggaatggc gcttggttga taaccgtcca ctttgacgcc ttgcggcaac
      901 ggtatttgtg ctttgtccgc gagtcgcaaa actcgctcgc ccgcgcgctg atcactgatg
      961 tctaccgttg ccacgagatc gagtgaagtc agatcgtcca acttgtttct gctgccggtg
     1021 agcatcacgt ccaccgttcg tggtggatcg ttgctaatct cgagtgacgg aggcctgatg
     1081 aagttgagct gaacgttgac gtgcgccgtg accggctcgt tctgtcccgt caccacgagc
     1141 cagagcacca gggcaatggc caacgagagc agtttcaagc tccagtcttc gagaaatatc
     1201 ttgcgtgcca acttctccac gcggctcggg gctggcgcgg gagctactgg ctcggcaact
     1261 tcgtctacgt cttgaaatgg catacagtca aacaggggcg atctcttccg cgtcctgatc
     1321 agtcgccttc tttagttcct tctcctttct tggcagcaca cccaaagcct cgaagatggc
     1381 gtggcgcaat ttggtcgcgt cgagaccgcg tttgatctcg cctttctgca cgaacgaaag
     1441 caaacctgtc tcttcggaga cgacgaccgc aaccgcgtca gtgtcttccg ttacaccgat
     1501 tgccgcgcgg tggcgcgtgc cgagatctcg ggagatacgc gggttgagcg tcaacggcag
     1561 aaagcacgac gccgctgcga tgcgatggcg gcggacgacg accgcgccgt catgcagtgg
     1621 acttgcagta ttgaaaatgc tcagcagcag gtcgtaactg agttccgcgt cgagctgcac
     1681 gccaccgtcg atgacgttct tcaacccgac gttacgctcg atcacgatca gcgcgcccgt
     1741 cttggtggag gcgagcgtcg tcgctgccag tattacttcg tcgtaaacgc cttcgccgaa
     1801 ttgcccgcga tgacgcgaga acggcatgcg aaatcgattg ccgaacgtga ttagagcctg
     1861 acgaatttcc gattgaaaca gaacgataat cgcgataccg atataaagca gtccgttgct
     1921 gagaacaaac tcgagtgtcg caagatcctg tttgatggaa agccagtaga gaaaaacgag
     1981 agcggcgatg ccggcggcca tgggcgcggc gcgagtaccg cgcaggagtt tcaagactgc
     2041 atacacgagc aagaagacga aaacgatgtc cgcgatattg cgcggctcaa cgagtcgtct
     2101 caaatactcc gtgtatggca gtagcagtaa catcttgtcg tgttatcaat tttggacgta
     2161 cctgatgctc gacgtagcga caccaactta gttctccact cgagtagcga gaataaaagc
     2221 tccgttcgga ttatcgcgga aaaatgcggc gcaatcaatc ggtgaatctg ttgaggtttt
     2281 aacgtgaatc tttcgcgcgt ttcttgcgcc tgaacttgcc gctttgcagg aaaccgacca
     2341 gttccccatt tttgccgacc acggccaacg aaccgattcc gttgcgtttc atcagttcgc
     2401 gggcataatc aagtctggcg gttggttcta caaagaatcg cggcgcgatt ggccgcatga
     2461 tgtcgcgcgc gcgggtgagg tgccagcgtt cgcgtggcaa cgacttcaaa tcctccagcg
     2521 acagcatccc gtgcagtcgc cgatcgcgcg ctactgggaa ggcgacctgg cggtgcatcg
     2581 gcaagacgga gtcgatcaaa ctactgatca acagatcagg ttcaatcgaa aacggcgccg
     2641 acatcgcctc gcgaacgatg ttctgcaacc gcccctgaac gtgcttcacg acgctgtacg
     2701 cggcgtcgaa aagaaaaaca cccacgagca cagaccagaa gcccatgaag taagaacgga
     2761 agtttggcgc aatcgccata tagaccccga agatgatcag gatcgcggcg atcagcatgc
     2821 cgcatattcc cgccattcgc gtcgcctggc gaatgtctcc ggtgcggcgc cagagaatgg
     2881 cgcgcaacac gcggccgccg tcgagtggat agcctgggaa gagattgaag accgccagca
     2941 ggagattacc ggcgcagaga agcagtaaaa ctccggcaac gatgtaatag ccggagcgca
     3001 ctgccggcag caggagcaca aaagtgactg cgccgatcaa aaaacttgcc gctggtccag
     3061 ccaccgcgat tctgaactcc gcgcgcgggt tctcaggctc gtggcgcaat cgtgccagac
     3121 cgccaaacgg atgcaagacg atctcttcga tttcgattcc ctctgaccgg cccatgaacg
     3181 cgtgcgagag ttcatggacc aacaccgaga gaaagagacc cagcgttgta attacaccga
     3241 gagtccacgc gacaacagtg cttaccggtt gtaggcgaaa tgaaccgacg aacatgcctc
     3301 cctggcggag gttcacggcg atcagccaga cgctcaaaag aaaaacgaca aaccaacggt
     3361 aatcgaaacg caccggaatg ccgaagatgc gcgctaccgt gatttggcgt ttataaaagt
     3421 ttctaaaact cacctttacg tctcgaaggc tgttgcttca gtgtctaaca acgggtgggg
     3481 ctccgtgcgc gcgacgcgcc gcagcaataa cggctcttca taaagttggg ccaaaccgcg
     3541 cacgatcgca aaacgaggat cgtcggccac tttgacgacc aggtcgagtt ctctttgcaa
     3601 gtactgcgac aaaccttcta acagcgcacc accgccggtc aatatcaggc cgcgttcata
     3661 aatgtcgccc gcgacttccg gcgcgagttc ggtcagcatc gagcgcactt cgtcggcaat
     3721 gcggcgaatg atcggcatcg ttatcgtgta gacctcaccg gccgtgatat cgaccgcggc
     3781 gggactgccg gtttgcacgt cgcgcccgcg cacgttgacc tttttgtttg gatcttcagg
     3841 ctcgattgcg gaactgagct caagtttcaa ccgttctgcc gtctgcgcgc cgatcgtgag
     3901 tccgcgatga cggcgcaagc ggtccatgat tgccacatcg atgtcgccgc tgccgatcct
     3961 ttcagcacgc gaagcgatga tcgacccgct gatgattgcg gcaacgttgg tggtgccgcc
     4021 gccgatatcc acgaccgccg cggcgcgttc gtcactgagc ggcacgcccg cgccgatcgc
     4081 cgccgcctgg ccttcttcga tcatgtagac gcgactgatg cgcgcctctt cggcggcagc
     4141 caacagcgcg cgttgctcga cctgcgtcac ttcagagatc acgctcataa tcgcgcgccg
     4201 gctaaagtgc gacacgccgc ttcgggcctg gcgaacgaaa tgatccagca tcaatctggt
     4261 tcgttcaaag tcggccacca cgccgtccac gagcggagcg atcacagtga cgtcgcgcgc
     4321 ttcgcggcct tgcattttgt gagcttcgtt gccgaaggca atgacttcgc cggtgagttt
     4381 gttgacggcc actaccgacg gttcatcgac gaccacaccc cggccacgca cggctatgag
     4441 ggtactcgcg gagcccatat cgatggccat ggagtcggaa acgaacttgc gcagcgcgct
     4501 aagtccaagg aagtgcccgg tcttacctat gaatgacata aaacttatag gtcctgactc
     4561 gaggacctgt ctagttatgc agtttgaaac cggggaccgc aggcgtctcg cctgcaaggt
     4621 gacgaatcgg gcgctctcgt gacatgcagg cgagacgcct gcggtcccag ggaagaacac
     4681 gccccggaaa tcggtgccgg gttcaaactg cctcacgagc cgtcaaaacg acccgcttgc
     4741 tttttgccaa tcgacctccc acagcatcac aggttgttgc ctgttcttca ccatgatcgg
     4801 ttcgcgtggc ttgagtttat aacggctacg cgcggccttg gcagtcgcat cgctgatcag
     4861 aatctctccg ccacgagcat tcgattcgag ccgcgacgag gtgttgacgg tgtcgccaat
     4921 ggccgtgtac tccgaacggc gttcagagcc gatatacccg acaatcactt cgccggtgtg
     4981 catacccatg ccgacgccaa tctctggaaa accctcgtca cgcaattccc gattgatacc
     5041 gaggagccgg cgctgcatgg ccacggcggc gttcaaggca tttgatgcgt catcgggcgt
     5101 tgttgttggc gcaccaaata gggccattag accatcgccg agatatttgt cgagtgtccc
     5161 gccgtgcgca aaaataatat cggtcatcgc cgagaagtaa cggttcagca ggctcacaat
     5221 cttttccggt ggagcatgtt ccgagatgcg tgtgaagccg cggatgtcgg caaaaaggat
     5281 ggtgatggtt tgactaacgc cgccgagctt gaatgaatcc gggttcaaca gcatctgctg
     5341 gacgacgtat tccggcaaaa accggctgta gttggcccgc gcgacttctt cgcgtgcgag
     5401 ccgttcgtgt gcgcgggcgt tttcgacggc gattgccgtt tgggccgcca cggcgctgat
     5461 cagttcgaga tcgtcaggct tgaaagcggc aaacggatcc aggcgatcgg cgtagagtgc
     5521 gccatggact cgcgactcgc ccatcaacgg cgcgcagatc gtcgagcgca caccttgcga
     5581 aacgatcgag tccacccctg caaactgttc gtcggaggcc gcgtcctgcg aaagcagcgc
     5641 aacgcggtcc ttcatcactt tccgcgtgat ggtgcggcca atagacagcc ggcgcgcgtg
     5701 ggcttcgagc ttgttgtcgc gtgcgcgcac ggcaatgggc gtcaggttaa cgtcgccacc
     5761 gtctgcatcg cccggttctg ccagcaacgc caccacgcga tcggctggcg tcgaacgaaa
     5821 gataatgtcg gtggccttct cgaagatggc gttcagatcg aagacggagc cgagcgtctt
     5881 actcatttcg taaagcgcgc tcagaatgtt tgctttgcgt tggagtgctt cgagaacttt
     5941 gtcgcgttgc gctgaagcag gcacgtcttc tttggcccgc aacacggtcg acatcacgtc
     6001 attagctgag attaagagtt gagtgtgccc aaggggtttg tcatcgtaac gaacgtcggt
     6061 agtccgttct gccgccggct gctcaaaacg caacgtgctc gcgccgatgg ttatgcgatc
     6121 gccatttttt agttgctgct cgaagcgtgg ctcaccgttg aggaagacct tgttggcgct
     6181 gcggcgaagc tgtccgttga ttatcgcgcc gtcgacaatc gtgaaggtcc cgttttctgc
     6241 ttgattgatg tgcgcgtgat aacgcgacgc gcgcgggtcg tccaaaacca ccgagttatc
     6301 cggagcgcgc ccgatcgtgc aggcagtctg cggcacaaaa gaaaactgcc actgacggcc
     6361 actggcgtcg gtgattttga gatgagccat ggcgcgcgat cctaacagta gattcttaag
     6421 gctgataacc gcgaaaaaat tctgtctcgc tagaccaggt ttcagttgcg gcgccggctt
     6481 atgtcgtcgc tgtcaaccaa atagaagttc attcagaacc acagcacatg aggtcgattc
     6541 aattttaaac aacagctgtt tacctgggc
//
LOCUS       DASTEY010000010         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_148411, whole genome shotgun sequence.
ACCESSION   DASTEY010000010 DASTEY010000000
VERSION     DASTEY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148411"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VFO72_00165"
     CDS             complement(<1..1827)
                     /locus_tag="VFO72_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9477723.1"
                     /translation="MNASLFLLLLFQTPSPSPLPTPPAPERTPALFTLIYVGGFALVI
                     LLLLLSLVRNRRQAVSAIPEDLPKEVRKRLGSHSTNRGLRALRWLFLLLSIGLFSFHI
                     YWAHYASEQNEKFQDLSYKDLRVRRLSESTLRGWVFDRSGRVDQPLAYYKRDSSGNIR
                     REYPMDKALAHLFGSDRGDPGLERALFGVQSGALPEALEVVKGQSVEFRGNQDVRLTI
                     DRALQQAAVEQLKGKHGAVVIVNPQNGDVLALYSEPSYSLTEVDDEATWIRLEANERD
                     KPLVSRALGAYYIPGSTFKTVTMIAAFIAGEQDTEFTCSGGGYYAAPGANVIFDDAGP
                     AEVHGRIGIDHAYEVSCNQYFAQMGVRLGPEKLKRAAQLLGIGSYDTPSEALRGRKLP
                     QIWAGSTDAVKRALAPRESTIVTGKQVTRYDLALIGYGQGYAGQMTPLQMALAAAAIG
                     NLEGKLMKPRIEYSVAPQPFNQVMTPQTAARMRSIMGLVTGGASGTARGVFGPVKAAG
                     INTGGKTGTAQKVVPVYDPKTGEPKTRHRVERDNRGNIIREYNEIIMDNENPRIDGWF
                     LCIAPLEKPQLAMAVIVEGGGYGSRSAAPIAAALVLKARDLGY"
     gene            complement(1837..>2040)
                     /locus_tag="VFO72_00170"
     CDS             complement(1837..>2040)
                     /locus_tag="VFO72_00170"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9477724.1"
                     /translation="SLNDQSVSKIHAALLMTAEGTLLVADTGSTNGTYLNGRRISYGE
                     SRLIEDGDVVGFGDVEVRLRKSE"
BASE COUNT          439 a          628 c          537 g          436 t
ORIGIN      
        1 atatccaaga tccctcgctt tcaaaacgag cgcggcggca attggggctg ccgatcggga
       61 gccgtagccg ccaccctcga cgatcaccgc catcgccagc tgcggtttct cgagcggcgc
      121 gatgcagaga aaccagccgt cgattctcgg attctcgttg tccatgatga tctcgttgta
      181 ttcgcgaatg atgttgccgc gattgtcgcg ctcgactctg tgtcgcgtct ttggctcgcc
      241 cgtctttgga tcataaacgg gcacgacttt ctgcgccgtt ccggtctttc cgccagtgtt
      301 gatgcccgct gccttgactg gtccaaacac gccccgcgcc gtaccactcg cgccaccagt
      361 aacgagcccc atgatcgaac gcattcgcgc ggcagtctgc ggtgtcatca cctgattgaa
      421 cggctgcggc gcgacgctgt attcaatccg cggcttcatc agcttgcctt cgagatttcc
      481 gattgcagcc gccgccagtg ccatctgcag cggcgtcatc tgaccggcat aaccctggcc
      541 atatccaatg agcgcgagat cgtaccttgt cacttgtttg cccgtgacga tagtggattc
      601 tcgcggcgcc agcgcacgtt tcactgcgtc cgtgctaccg gcccaaatct gcggtagctt
      661 gcgaccacgc aacgcttctg aaggcgtgtc gtacgagccg atgcccagca actgtgcggc
      721 gcgtttaagt ttttccggac caagcctcac acccatctgc gcgaaatact ggttacagga
      781 aacttcatac gcatgatcga tcccgatgcg accgtgtacc tccgctggcc cagcatcgtc
      841 gaagattacg ttcgcacctg gcgcggcgta gtaaccacca ccactgcacg taaactccgt
      901 atcctgctct ccggcgataa acgccgcgat catcgtcacg gtcttgaaag tcgatccggg
      961 aatgtaataa gcaccgagag cacgactgac cagcggtttg tcgcgttcat ttgcttccaa
     1021 acgaatccac gtcgcttcgt cgtcgacttc agtaagactg tacgaaggct cgctgtagag
     1081 cgcgagcacg tcaccgtttt gcggattcac gataacaacc gcgccgtgct ttcctttcaa
     1141 ctgctcgaca gcagcttgtt gcaaagcacg atcaatcgtg agtcgcacgt cctgattgcc
     1201 cctgaactcg accgactgac ccttaacaac ctcaagagct tcgggaagcg cgccgctctg
     1261 aacgccaaac aacgcgcgct ccagtcccgg atcgccgcga tccgatccga agagatgcgc
     1321 caatgctttg tccatcggat actcgcgccg gatattgccg ctcgagtcgc gtttgtaata
     1381 agccagtggc tggtccacgc ggccgctgcg atcaaacacc catccccgca gcgtcgattc
     1441 agacaaacgc cggacgcgca gatccttgta gctcaagtcc tggaacttct cgttttgttc
     1501 cgacgcgtag tgggcccagt agatgtgaaa cgaaaacaac ccgatcgaca acagcagaaa
     1561 caaccatcgc agcgcacgca aaccgcgatt agttgagtgc gatccgagtc gcttcctgac
     1621 ttctttcggc aggtcttcag gaatcgccga tactgcctgg cgccgattgc gaaccagact
     1681 aagcagcagg agcaaaatga cgagcgcaaa cccgccgacg taaatcagcg tgaacagagc
     1741 cggcgttctt tcgggcgcag gcggagtcgg gaggggggaa ggtgaagggg tttggaagag
     1801 aagtagaaga aaaagactcg cgttcatcca gtttcgctac tcacttttgc gcaatcgtac
     1861 ttccacatct ccaaagccaa ctacgtcgcc atcttcaatc agccgtgatt cgccatatga
     1921 gatgcgtcgt ccgttgagat aagtaccatt cgtagagccg gtgtcggcaa cgaggagcgt
     1981 gccttcggca gtcatcaaca gcgcggcatg aattttagac acgctctgat cgttcagaga
//
LOCUS       DASTEY010000011         7690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1120784, whole genome shotgun sequence.
ACCESSION   DASTEY010000011 DASTEY010000000
VERSION     DASTEY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120784"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFO72_00175"
     CDS             <1..687
                     /locus_tag="VFO72_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477725.1"
                     /translation="HAMQLWLNRRFSKRLAFQVAYTWGHAISDVALTSFTNTTSDPFN
                     FKADKGDADLDRRHTFVSNVVYVLPSAKRWGRAADLILSDWQINAIASYFGATPIDVI
                     TGANTIGTAAAVGQRPNYTGAPLYLDTGDSTRHLNPAAFARPAPGQLGTLGKGAVRGK
                     PITTVDFSLAKNWRFHERYGIQFRTEFFNLLNHTNFVGFDTDIRNLSFGTLNAAQAPR
                     EIQLGIKFTF"
     gene            756..2981
                     /locus_tag="VFO72_00180"
     CDS             756..2981
                     /locus_tag="VFO72_00180"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9477726.1"
                     /translation="MSSIGVTMYLRSAFLPLLLSVFAFQAQGDLFRRHYEAAQNYHRA
                     GNHAAAEAEFKIILAESYLRLGRIYSAQGKYQPASDVLKTATSIRPGATEGLVELSIA
                     YFHLGQFSKAIEPLQRAIAADARNAAAHHMLGKSYFMMGEFEKAAAELQTALKLSPDD
                     YDAEYTLGLAYLKRKDTASAKQLYASMAQRLGNRPALRVLIGRAYRETGFLAESIEEF
                     KAAIALDPRFPRVHYYLGLTYLYKDGAARIPEAMEEFKIELAANPEEYFANFYLGILY
                     IMERKWDEAIALLEKAVRKQPNNPDPYFHLGQAYQGAGKHQQAVAVLQKTIALNPSLD
                     HNDYQVTTAHYRLGQSLMKIGRTAEGQKELQTSAELKSKGFKMDEKKVGAFLSGARPP
                     DQEGNLVKAEGVVAESVALDVKTIEKLKADETLFSKVAAAAHNSIGLLRAEQQDFREA
                     AVQFAAAAKLNPSQQGLNYNLGLAYYKSEAYKDAIPALENELKVDPNNVGIKQLLGLS
                     YFMTENYAKAASLLEAVVAVKTQEAALYFPYALSLLKDGKLEAATRAIEQMVLVGGNS
                     PQLHILLSQANYERGDTAKALEELQAALALDDKVRLAHFYAGLIHLKDGKMDLAAREF
                     ESELALNPNDLQAKYHLAFVALAQQQIERGLKLMREVIRERPDFADAYYELGKALLQQ
                     GDVAGSVENLETAARLKPNQAHVHYQLGRAYMAAGRKKEGESQLEISRELKEKARAKG
                     N"
     gene            2999..4663
                     /locus_tag="VFO72_00185"
     CDS             2999..4663
                     /locus_tag="VFO72_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HET9477727.1"
                     /translation="MSLKLIVLLVAFTLVPSQKVRFTDITAQAGITFKHVASPEKKYI
                     VESMSGGVALFDYDNDGDLDIYLVNSLTVDLVKSKGKTKSDLYRNDGNGKFTEVGQKA
                     GVSDIGWGMGVATGDFNNDGFEDFYVTCLGPDHLFKNNGNGTFTDVTSKAGVSDPRWS
                     TGASFVDYDRDGDLDLFVANYVDFDINNLPEFGQGQTCQYKSIPVQCGPRGLKGAGDS
                     LFRNNGNGTFTDVTKQAGVADADGFYGLGVMTSDFDDDGLIDIFVANDSTPNFHYRNN
                     GDGTFKEIGFTAGTAVNENGSEQGCMGVTAGDYNHDGKIDLFVTNFADEYNTLYHNDG
                     SNSFTDISYAAKVAAVSLPHVGWGTKFFDYDNDGWVDLFVANGHVYPQLPSYRQPRLL
                     HRNNRDGTFTEVSAEFGALLTELRASRGIAFGDIDNDGDIDLIVADLDGPPQLLRNDN
                     GNANNSILIKTVGAKSNRSGIGARVKVVAGDLTQVDEVRSGDSYISQSDVRLHFGLER
                     RTKVDLIEIRWPSGNVEKITGVGVNRVITVREGQGKVDEKEFRSSE"
     gene            4708..5904
                     /locus_tag="VFO72_00190"
     CDS             4708..5904
                     /locus_tag="VFO72_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9477728.1"
                     /translation="MAQDDKLAARLEHVASLIRENKTIEAERELTGFLRTNPNQPVAL
                     NLLGTIRAKQGRLNEAETLFVRAVNHNTRFSGARMNLAYLYMQKREPAKAVVQLKEVL
                     VAEPANTEVMVVLGDAHLAAGALAEAEEQYLLALEGRLDNAAALLGLAQISRLKGELK
                     EATLLLTRVGTLTADSKSPEFLYKFAGVAMQVGMSDAAKAALENALKLRPNDPAYLLA
                     YGIAWLRKGDLFEAEKVFRRLLQLKPNSDQAQIHLGYVLLNQKKYDEARLWLEKSARS
                     TAPMPEVFYYLGLVAQEQNDDAKAIALFEKAVQKLPNYAHARIALGSSYIRLRNYPRA
                     KEELETAVKLDPEEPTAHYNLALLYARLKDPARAQEEMRIVEKLKAKGAGSDGVVVLP
                     PNPNPR"
     gene            5907..7529
                     /locus_tag="VFO72_00195"
     CDS             5907..7529
                     /locus_tag="VFO72_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HET9477729.1"
                     /translation="MKSLFLVLILLASITFEEVSPQTSKITWAHNNAQSADRQLPETV
                     GAGCAFLDYDNDGWMDIYFVNSGPSDFFTPKTPLKNALYRNNGDGTFTDVTDKAGVAG
                     GKFGMGVAAADFDADGFVDLYVTNYGPNILYRNNGNGTFTDVTDKAGVVSPEWSTCAT
                     WFDFDNDGKLDLFVSSFVFYDKSQNPLCTDPTLQRRYYCVPRLFKPTPSRLFRNNGNG
                     TFQDVSRESGIGAHPGKSFGAVATDVNNDGMMDLFVANDTMPNFLFVNKGSGKFEEIG
                     LAAGVAYGEAGRPRSGMGVDAADYDGDGWQDLFVANIDHEFFSLYHNDKELIFTDQPG
                     EIAPATQLLSGWGLKFFDYDNDGDPDLFLVNGHPDDMIESRIPRVRHREPLLLFENTG
                     RGFKDVSARSGAVFSKMFSGRGMAVGDFDNDGDADVLASNNGEPPLLLRNQGGNKNNW
                     VGLNLVATKSNSAAVGAVITWQAGATKRTRLRTGGGSYLSSHDPREILGVGAAKKIDS
                     IEIRWPSGATDRLTNVPLNKYIRVVEGAGQSR"
BASE COUNT         1795 a         2124 c         2186 g         1585 t
ORIGIN      
        1 cacgcgatgc agttgtggct caatcgccgc ttcagcaaac gcctagcgtt ccaggttgcc
       61 tacacctggg gtcacgcgat ctcagacgtt gcgctcacgt cgttcaccaa cacgaccagc
      121 gatccgttta acttcaaagc cgacaagggc gatgcggatc tcgatcgccg tcatacattt
      181 gtcagcaacg tggtgtacgt cttgccctcc gcgaagcgtt ggggacgagc ggcggatctc
      241 atcctgagtg attggcagat caacgcgatt gcttcgtact tcggggcgac tccgatcgat
      301 gtaattacgg gcgccaatac catcggaacg gcggcggctg tgggccaacg tccgaactat
      361 actggtgcgc cgctttatct cgacaccggc gactccacca gacatctgaa cccggcagcg
      421 ttcgcaagac ctgcccctgg tcagttgggt acgttgggga agggcgccgt gcgcggtaag
      481 ccgatcacta cggtcgactt ttcactcgcc aagaattgga gattccatga gcgttacgga
      541 atccagttcc gcacggagtt cttcaatctc ttgaaccaca cgaactttgt tggctttgat
      601 accgacatcc gcaacctgtc gtttgggacg ctcaacgcag cccaagcgcc gcgcgagatt
      661 caactcggta tcaagtttac cttctgatat actgccggca gtctaaataa ggacgaagcg
      721 ctgagagatc cccctcagct cttcgtccaa gttttttgag ttctattggg gtgacgatgt
      781 acctgcgctc ggcctttctc cctctcttac tttcagtttt tgcttttcaa gctcagggcg
      841 atctgtttcg ccgtcactac gaggcggcgc aaaattatca ccgcgccggc aaccacgccg
      901 cggccgaagc cgagtttaag atcattcttg ctgagtcgta cctccggctc ggcagaatct
      961 actcagcgca ggggaaatac caacccgcgt ctgacgtact gaaaacagca acatccatcc
     1021 ggccgggtgc gacagaagga ctcgtcgaac tctccatcgc ttacttccat ctgggccaat
     1081 tctcgaaagc aatcgaaccg ttgcagcgcg cgatcgctgc cgatgcgcga aacgccgcgg
     1141 cgcatcatat gctcgggaag tcgtatttca tgatgggcga atttgaaaaa gcagcagccg
     1201 agttgcaaac ggcgttgaag ttatcgcccg acgactacga tgccgaatac acgttgggcc
     1261 tcgcctatct gaagcggaaa gacacggctt cggcgaaaca gttgtacgcg tcgatggccc
     1321 aacgtctcgg caatcgtccg gccctgcgtg tgctcatcgg ccgcgcgtat cgagaaaccg
     1381 gatttctggc ggagtcgatt gaagaattca aagctgcgat cgcgctcgat ccacgctttc
     1441 cccgcgttca ttactatctc ggcctgacgt atctctacaa agacggcgcg gcaaggattc
     1501 ccgaggcaat ggaggagttc aagatcgaac tcgccgccaa tccggaggag tacttcgcaa
     1561 acttctatct cggcattctt tacatcatgg aacggaagtg ggatgaagcg atcgcgttac
     1621 tcgagaaggc ggttcgcaag cagccgaaca atcccgatcc atatttccac ctggggcagg
     1681 cttatcaggg cgcaggcaag caccagcagg ctgtcgcggt gttgcagaag acgattgcgc
     1741 tgaatccatc gctggatcac aacgactatc aggtgacgac tgcgcattac cgtctcgggc
     1801 agtcattaat gaaaatcggc cgcactgccg aaggtcaaaa agaactgcaa acgtctgccg
     1861 agttgaagtc gaagggcttc aagatggacg agaagaaagt cggcgcgttt ctcagtggtg
     1921 ctcgaccgcc ggatcaggaa ggtaatctcg tgaaggcgga aggtgtcgtt gctgaatccg
     1981 ttgctctcga cgtgaagacc atcgagaaac tcaaggccga cgagactttg ttttcgaaag
     2041 tggcggccgc ggcacacaac agcatcggtt tgttgcgcgc tgaacagcag gactttcgcg
     2101 aggcggcagt gcagtttgcg gcggctgcga agttgaatcc gagccagcag ggtctcaatt
     2161 acaatctcgg cctcgcctac tacaaatcag aagcatacaa ggacgcgatc ccggcgctcg
     2221 agaacgagct caaagtcgat ccaaacaacg ttggtatcaa gcagttgttg ggactcagtt
     2281 acttcatgac cgaaaactac gccaaagcag cgtctttgct ggaggcggtt gtcgcggtca
     2341 aaactcagga agctgcgctc tactttccgt atgctctttc gctgctgaag gatggcaagc
     2401 tcgaagctgc cacgcgcgcg atcgaacaga tggtgcttgt cggtggcaac agtccgcagt
     2461 tacacatcct gttaagccag gccaactacg aacgtggcga cactgcgaaa gcactcgaag
     2521 agctgcaagc tgcactcgcg ctcgacgaca aagtgcgtct ggcgcatttc tatgccggct
     2581 tgattcattt gaaggacggg aaaatggatc tcgcggcgcg cgaattcgag agcgagttag
     2641 cgttgaatcc gaatgacctg caggcgaagt atcatttggc atttgtggcg ttggcccagc
     2701 aacagatcga gcgcgggctg aagttgatgc gtgaggtcat tcgcgagcgt cccgattttg
     2761 ctgacgcata ctacgaactc ggcaaagcgt tattgcagca aggcgacgtc gccggcagtg
     2821 ttgaaaatct cgagacggcc gcgcggctca aacccaatca ggcccacgtg cattatcagt
     2881 tgggccgcgc ttacatggcg gccggccgca agaaagaagg cgaaagccag ctcgaaatct
     2941 cgcgcgaatt aaaggagaag gcgcgggcca aaggtaattg aggccgcaga ttacaaagat
     3001 gtccttgaaa ctgatcgtct tactcgtggc ctttactctt gtgccttctc aaaaggtgcg
     3061 gttcacggac atcaccgcac aagccggaat caccttcaaa catgtggctt cgccggaaaa
     3121 gaaatatatc gtcgagtcga tgagcggcgg cgtggcgttg tttgattacg acaacgacgg
     3181 cgatctcgac atttatctcg tcaactcact gacagtcgat ctggtgaagt cgaaaggcaa
     3241 aacaaaaagc gatctatatc gcaatgacgg gaacggcaag ttcactgaag tcggtcagaa
     3301 agccggtgta agcgatattg gttggggcat gggtgtcgcc actggcgatt tcaacaacga
     3361 tggcttcgaa gacttttacg ttacgtgtct cggtccggac catcttttca agaacaacgg
     3421 caacggcaca ttcaccgacg tcacgagcaa agcaggcgtg agcgatccac gctggtccac
     3481 cggcgcttcg ttcgtcgatt acgaccgcga cggcgatctc gatctgtttg tcgcgaacta
     3541 cgtcgacttc gacatcaaca atctacccga gtttggccag ggccagacgt gccagtacaa
     3601 gtcgattccc gtccagtgtg gtccacgagg gttgaaaggt gcgggcgact cactttttcg
     3661 caacaacggc aatggcacgt ttacagatgt cacgaagcag gcgggtgttg cggacgccga
     3721 tggattctac gggctcggtg tgatgacgag cgacttcgac gacgatggtc tgatcgatat
     3781 cttcgtcgcg aatgattcca cgcccaactt tcactatcgc aacaacggcg acggcacgtt
     3841 caaagagatt gggtttacgg ccggcacggc ggtaaacgag aacggcagcg agcagggctg
     3901 catgggcgtc acggcgggcg attacaacca cgacggcaag atagatctgt ttgtgacgaa
     3961 tttcgcggac gagtacaaca cgctctatca caacgacggg tcaaactcat ttaccgatat
     4021 ctcgtacgcg gcgaaggtcg cggccgtgag tttgccgcac gtgggttggg gcacgaagtt
     4081 tttcgactac gacaacgatg gctgggtgga tctgtttgtc gccaacgggc acgtttatcc
     4141 gcagcttccg agttatcgcc agccgcgctt gctgcaccgg aacaaccgcg acggcacgtt
     4201 taccgaagtc tcggcggaat ttggcgccct attgacagag cttcgtgcga gccgtggtat
     4261 tgcattcggc gatatcgaca acgacggcga catcgatctc atagttgccg acctcgacgg
     4321 tccgccacag ctattgcgca atgacaacgg aaacgcgaat aattcgattc tcatcaagac
     4381 agtgggggcg aaaagcaatc ggagcggcat cggcgcgcgt gtgaaagtcg ttgcgggcga
     4441 cctgacgcag gttgacgagg tgcgcagtgg cgacagttac atttcgcaaa gcgacgtgag
     4501 attgcacttc ggactcgagc ggcgaacaaa agttgacctg attgaaatcc gttggcccag
     4561 cggcaatgtc gagaagatca ccggcgtcgg cgtaaaccgc gtcatcacgg tcagagaagg
     4621 tcagggcaaa gttgacgaga aggagtttag gtcatccgaa taagtccgtg gaaatcgttg
     4681 ttggtagtgc ttttcgcctc tgttgctatg gcgcaagacg ataaactcgc cgctcgtctc
     4741 gaacacgttg cgtccctgat tcgcgaaaac aaaacaatcg aagccgaaag agagttaacc
     4801 ggatttctcc gcaccaaccc caaccagcct gtggcgctaa acctgctcgg cacgattcgc
     4861 gccaaacaag gccggctcaa cgaagccgaa acgttgtttg tgcgcgccgt caaccacaac
     4921 acacgcttct ccggcgcgcg catgaatctc gcgtacctct acatgcaaaa gcgcgaacct
     4981 gctaaagcgg tggtccaact gaaagaagtg cttgtcgctg agcccgccaa cacggaggtt
     5041 atggtcgtgc tcggcgacgc gcatctggcg gccggcgcgc tagccgaagc tgaagaacaa
     5101 tatctactcg cgcttgaagg gcgtcttgac aatgccgccg cgctgctcgg tctcgctcag
     5161 atctcgcgat taaaaggcga gcttaaagaa gcgacgcttt tgctgacgcg tgtcggcacg
     5221 ttgacggccg attcaaagtc acccgaattt ctctataagt ttgcaggcgt cgcgatgcag
     5281 gtcgggatga gtgatgctgc aaaagcagcg ctggaaaatg cactgaaact gcggcccaac
     5341 gatccggctt acctactagc ttacggcatc gcgtggctgc gaaaaggcga tctgtttgaa
     5401 gcggagaaag tgtttcgccg cttgctccaa ctgaaaccaa acagcgatca ggcgcagatc
     5461 catttgggtt acgtgctgct gaatcagaag aagtacgacg aagcgcggct gtggctcgag
     5521 aaaagcgcca gatcgactgc gccgatgccg gaggtgttct attacctcgg cctcgtggcc
     5581 caggagcaga acgacgatgc gaaagcgatc gcgttgtttg aaaaggccgt gcagaaactc
     5641 cctaactacg ctcatgcgcg cattgctctc ggttcgagtt atataagact gcgcaactat
     5701 ccgcgcgcga aagaggagtt ggagacggcg gtcaaactcg accccgaaga accgaccgca
     5761 cattacaatc tcgccctgct ctatgcgcgc ttgaaggatc ccgcgcgcgc gcaggaagag
     5821 atgcgcatcg tcgaaaagct gaaagcgaaa ggtgccggaa gcgatggcgt ggtggtgctg
     5881 ccgcccaatc ctaatccacg ttgatcatga aatccctgtt cctggtactt atacttcttg
     5941 cctcgatcac ctttgaagag gtctcaccgc aaacaagcaa gatcacctgg gcccacaaca
     6001 acgctcagtc ggcagatcgc cagttaccgg aaacggtcgg cgcgggttgc gcttttctcg
     6061 actacgacaa cgacggctgg atggacatct atttcgtcaa cagtggtcca tccgatttct
     6121 tcacgcccaa gacgccacta aagaacgccc tctaccgtaa caatggtgac ggcactttca
     6181 ccgacgtcac cgacaaagcg ggagtcgccg gtggaaagtt tggcatgggc gtggccgcgg
     6241 ccgatttcga cgcagatggt ttcgtcgatc tctacgttac gaactacggg ccaaacattc
     6301 tctaccgcaa caacggcaac ggcaccttca ccgacgtcac tgacaaagca ggcgtcgtgt
     6361 cgccggaatg gtccacctgc gcgacgtggt ttgatttcga taacgacgga aaactggatc
     6421 tgtttgtctc gagcttcgtt ttttacgaca agtcgcagaa cccgctctgc accgatccga
     6481 cactgcaacg ccggtactac tgcgtgcctc gtcttttcaa accaacgccg tcgcgcctgt
     6541 tccgcaacaa cggcaacggt acgtttcagg acgtgagtcg cgaatccggc atcggcgcgc
     6601 atccgggcaa atcatttggc gccgttgcca ctgacgtgaa caacgatgga atgatggatc
     6661 tcttcgtcgc gaatgacacg atgcccaact ttcttttcgt caacaaaggc agcggcaagt
     6721 ttgaggagat cggtttggct gcgggtgttg cctacggtga agcgggccgg ccgcgttcgg
     6781 ggatgggcgt ggatgcagcc gattacgacg gcgatggttg gcaggatctt ttcgtcgcca
     6841 acatcgatca cgaattcttc agtctctatc acaacgacaa ggagttgatc ttcaccgatc
     6901 agcccggcga gatcgcgccg gcgacgcagc tattgagtgg ttggggtttg aagttcttcg
     6961 actacgacaa cgacggtgac cccgatctgt ttctcgtcaa cggccatccc gacgacatga
     7021 tcgagtcgcg cattccacgc gtcaggcaca gggagccgct gctgcttttc gagaatacgg
     7081 gccgtggttt caaagacgtg agtgcgagat ccggcgcggt tttcagcaag atgttttcgg
     7141 gtcgcgggat ggccgtcggt gatttcgaca acgatggtga tgcggacgtg cttgcttcga
     7201 acaatggcga gccgccgcta ctgctgcgaa accagggcgg caacaaaaac aactgggtcg
     7261 gattgaatct tgttgcgaca aagagcaact ccgcggccgt tggcgcggtg attacctggc
     7321 aggctggcgc gacaaaacgg actcgcctgc gcacgggcgg cggcagttat ctttcgtcac
     7381 acgatccgcg ggagattctc ggcgttgggg ccgcgaaaaa gatagattcg attgagatac
     7441 gttggcccag cggcgccacg gataggctca caaacgtacc gcttaacaaa tatataagag
     7501 tcgtggaagg cgcgggccag agccgttaac cctaccacgc ctcgtagaac caagacgtac
     7561 cttgtatcct gggaccgcag gcgcctcgcc tgcatgactc gccacaagcc tctttcggca
     7621 gcagtgcagg cgagacgcct gcgctcccag gaaaaagacg ccccccagga aaagacgccc
     7681 cccaggaaaa
//
LOCUS       DASTEY010000012         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1236183, whole genome shotgun sequence.
ACCESSION   DASTEY010000012 DASTEY010000000
VERSION     DASTEY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236183"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFO72_00200"
     CDS             <1..209
                     /locus_tag="VFO72_00200"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477730.1"
                     /translation="DKLALVPEGLSQAESAVFRLLNHDSPAHVDWLVDNSKLPISELT
                     AALLALEMRELVRALPGRCFVRKL"
     gene            210..2726
                     /gene="topA"
                     /locus_tag="VFO72_00205"
     CDS             210..2726
                     /gene="topA"
                     /locus_tag="VFO72_00205"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9477731.1"
                     /translation="MSKNLVIVESPAKAKTINKYLGKDYVVKASIGHIKDLPSKGLGV
                     DVEHNFEPTYELIPDSKKRNNKKIVAELKKAAKEADAIYLAADPDREGEAICQHLKEE
                     IVPKRPAKPAYRVMFNEITKRAVNAAFEHPRQIDENLVEAQQARRVLDRLVGYKVSPL
                     LCRTIGGRLSAGRVQSVALRMVVEREREIEQFVKTEYWTIGANLSAKLPPAFDARLFK
                     VGEQTVKTSGFDQDLKKNEILIQDQTQAEEFVAEAQQQSFVVSEVTTRERKRNPVPPF
                     ITSKLQQEASRKFGFPVKKTMMLAQKLYEGVELGAEGSVGLITYMRTDSTRVSDAALG
                     EVRDFINQQYGGNYLPEKAVHYRSKKDAQDAHEAIRPTEVGRTPDSLARFLKPDELKL
                     YRLIWQRFVASQMTAAIFDQTTIDIQAGRFVFRATGSVQKFDGFLKIYQEGRDEKSED
                     DDEAERSLPLVEEGETLKLNSVTPEQHFTEPPPRYTEATLVKALEEKGIGRPSTYAAI
                     MSTIQEREYVEKIQSRFHPTALGKTVNDLLIEGGFDDLFNETYTARMEEELDEIEEGK
                     LQWTKALREFYKKFEGDLKEFQRYIKDIKEKSVPTEEVCLKCNTPGMVQKWGRFGPYL
                     KCLNCEATRDAEPPAGSDGAGAATEGANGGEETEPETCELCGKPMQLKRGRFGPFLGC
                     TGYPECRNIRKIGKTGVAAPAPVPLDEKCPVDGAQLVKRFGRYGEFISCSNYPKCKYI
                     KQETVGVSCPRQGCKGEIVVKKSKRGKAFYGCSEYPNCNVVYWDKPVLDTCPQCNAPF
                     LLEKTTKKQGTFRYCANQECGYRSNQPLETTEKPRSVLHR"
BASE COUNT          743 a          777 c          829 g          556 t
ORIGIN      
        1 ccgataagct cgcgcttgtg cccgaaggat tatcacaggc agagagcgct gtgtttcggt
       61 tgctgaatca cgacagtccg gcacacgtcg actggctggt cgataacagt aaacttccga
      121 tttcggaatt gaccgccgcg cttttggcgt tggaaatgcg agagctggtg cgtgcgctgc
      181 ccggaagatg ctttgtgaga aaactataaa tgtcaaaaaa cctggtaata gttgaatcgc
      241 ctgcaaaggc aaaaaccata aacaagtacc tgggaaagga ctacgttgtt aaggcctcga
      301 taggccacat caaagatctg ccttcaaagg gactcggagt agacgtggaa cacaacttcg
      361 aacccactta cgaactgatt cccgattcga agaaacgcaa caacaaaaag atcgtcgccg
      421 aactgaagaa ggctgcgaaa gaagcagacg cgatctatct cgcggctgac ccggatcgcg
      481 aaggcgaagc gatctgccag cacttgaaag aagagattgt gccgaagcgt ccggcgaagc
      541 ccgcctatcg cgtgatgttc aatgagatca caaaacgtgc ggtcaatgct gcgttcgaac
      601 atccgcgaca gatcgacgaa aacctcgtcg aggcgcagca ggcgcgccgc gtgctcgata
      661 gactcgtcgg ttacaaagtc tcaccgttgt tgtgtcgcac gatcggcggc cgtttgagtg
      721 ccggacgcgt gcagtcagtt gcgctacgca tggttgttga gcgcgaacgc gagatcgagc
      781 agttcgtcaa aaccgaatat tggaccatcg gcgcgaatct cagcgcgaaa cttccgcccg
      841 cgtttgacgc gcgactgttc aaagtcggcg agcaaacagt caagacgagc ggcttcgatc
      901 aggatctcaa aaagaacgag attctgatcc aggaccagac gcaagccgag gaattcgtcg
      961 ctgaagcgca gcagcagtcg tttgttgttt ccgaggtcac gaccagggag cgcaaacgta
     1021 atccggttcc gcctttcatc acctccaaac tccagcagga ggcttcgcgc aagttcggtt
     1081 ttcccgtcaa aaagacgatg atgctggcgc agaaactgta cgaaggtgtc gaactcggcg
     1141 cggaaggttc ggtcggtctg atcacttaca tgcgtaccga ctcgacgcga gtttcagacg
     1201 ctgcgctcgg cgaagtgcgc gacttcatca accaacaata cggcgggaac tacttgccgg
     1261 aaaaggcagt ccactatcgc tcgaagaaag acgctcagga tgcgcacgaa gcgatccgtc
     1321 ccacggaagt cggccgcacg cccgactcgc tcgccaggtt tctcaagccc gacgagctaa
     1381 aactctatcg tttgatctgg cagcgtttcg ttgcttcgca gatgaccgcg gcgatcttcg
     1441 atcagactac gatcgatatt caggccggac ggtttgtctt tcgcgccacc ggttcggtgc
     1501 agaagttcga tggctttttg aagatctacc aggaaggtcg cgacgaaaag agcgaagacg
     1561 atgacgaagc agaacgtagt ctgccgctgg tagaagaagg cgaaactctg aagctcaatt
     1621 cggtcacgcc cgagcagcac ttcacagaac cgccaccgcg ttacacggag gcaacgctgg
     1681 tgaaagcgct cgaagagaag ggcatcggcc gcccgtcaac ttacgccgcg atcatgtcga
     1741 cgattcagga gcgcgagtac gtcgaaaaga ttcagtctcg ttttcatcca actgcgcttg
     1801 gcaagaccgt caacgatctt ttgatcgaag gcggctttga cgaccttttc aacgagactt
     1861 acaccgcgcg catggaagag gaactcgacg agatcgagga aggaaaactt cagtggacca
     1921 aggcgctgcg cgagttctac aagaaatttg agggcgacct gaaagagttt cagcgttaca
     1981 tcaaagacat caaggaaaag agcgtgccga ccgaagaggt gtgtttgaag tgcaacacgc
     2041 ccgggatggt ccagaaatgg ggccggttcg gtccgtacct caagtgtctc aactgcgaag
     2101 ctacgcgtga cgcggagccg ccggccggat cggacggcgc cggagcggcg acagaaggag
     2161 cgaacggtgg cgaagaaacg gaacccgaaa cctgtgaatt gtgcggcaag ccgatgcagt
     2221 tgaagcgcgg gcgctttggt ccattccttg gctgcacggg atatcccgag tgccgcaaca
     2281 ttcgcaagat cggcaagacg ggcgtggctg cgccggcgcc ggtgccgctc gacgagaagt
     2341 gtccggttga cggcgcgcaa cttgtcaaac gcttcggacg ctatggcgag tttatctcgt
     2401 gttcgaatta tccgaagtgc aaatacatca agcaggagac ggtcggtgtt tcctgtcctc
     2461 gtcagggctg caagggcgag atcgtggtga agaagtcgaa gcgcggcaaa gctttttacg
     2521 gctgttcgga gtatccaaac tgtaacgtgg tctactggga caagcctgtg ctcgacacgt
     2581 gtccacaatg caatgcaccg ttcctgctgg aaaagaccac gaaaaagcag ggcacgtttc
     2641 gctattgcgc gaaccaagaa tgtggctatc gttcgaatca gcctctcgag actaccgaaa
     2701 aaccgagatc tgtgttacac agatgacacg gattccaaac cagtaagccc aaactgaaat
     2761 cgaggagacc caccatgatt gttcggcgct atacagcaat agtgctgctt ttaatccttg
     2821 tactgtgccc ggcgacatac ctggcccaaa ccgctcaacc cccgccggcg ccagatccaa
     2881 acgaccctat ccagcgcatc aagga
//
LOCUS       DASTEY010000013         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1054888, whole genome shotgun sequence.
ACCESSION   DASTEY010000013 DASTEY010000000
VERSION     DASTEY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054888"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFO72_00210"
     CDS             complement(<1..388)
                     /locus_tag="VFO72_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HET9477732.1"
                     /translation="MSIQRNDWSLQRKGIIDQERHKDRVKEAIRKNLGSIVSNEAIIL
                     SDGKRTVKVPIRALDEYKFRFNYQKSKHVGQGDGKTRVGDVIGRESRPGSGQGSGQAG
                     QDAGADYYEAEVNIDELAALIFEDLQL"
     gene            complement(395..1348)
                     /locus_tag="VFO72_00215"
     CDS             complement(395..1348)
                     /locus_tag="VFO72_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9477733.1"
                     /translation="MKMKKRYWIAGTATAAATIAVAAKLLTRPRDADWQKYRHVVFHS
                     EHSRFVEIDGVRVHYQEAGDRSNPVLVLIHGFASSTLVWSKVFLKLAQAGFRVIAVDL
                     LGYGYSGKPRNGEYTIAGQASLLIRLLERLGIKRATLVGSSYGGAVAATCALDHPHRV
                     EKLVLVGTVNNNEPLRFKVMRIFSSRVVGDVVSPLLIGSRRLLRKRMKRVYDRHAWVL
                     DERRVDARHFPLRASGTQRAIIRTVRGWNAERISSDAHLIRQPTLLLWGENDLEIPLA
                     DGERLHQEIPGSRLVVFLACGHLPHEEYPEAFTKVVADFCG"
     gene            complement(1431..>2318)
                     /locus_tag="VFO72_00220"
     CDS             complement(1431..>2318)
                     /locus_tag="VFO72_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477734.1"
                     /translation="HQSEALRNVHLAPNTLRVAAMFAVMTRLEEPKRQNVDIVKKMKL
                     YDGEDVEGYKSKDVRELKEETVREGMDGISPRYIINRLSSALVRDGVTCINPIDALRA
                     IKDGFEQHTGISAEQRERYLNHISQARKEYDELAKIEVQRAFVYSFEEMARTMCNNYL
                     DNVEAFCNKERIKDPITEEEMDPDEQLMRSIEEQIGISENAKNTFRQEILIRISSYAR
                     KGKPFEYNSHERLKEAIEKKIFADLKDVVKITTSAKTPDPDQLRRINEVVDRLVKEHG
                     YCPVCANELLTYVGTLLSR"
BASE COUNT          471 a          669 c          611 g          567 t
ORIGIN      
        1 gaagttggag atcctcgaag atcagtgcgg cgagctcgtc gatgtttact tcggcttcgt
       61 aatagtcagc acccgcatcc tgcccggcct gtcccgatcc ctgaccgctt ccgggccggc
      121 tctcgcgacc gataacgtcg ccgacgcgcg tctttccgtc gccctggccc acgtgtttgg
      181 atttttgata gttgaagcga aacttgtatt cgtcgagcgc tctgatggga acttttacgg
      241 tgcgtttccc atcggacaga atgattgctt cattggagac gatcgatccg aggtttttac
      301 ggatggcttc cttgacgcga tccttgtggc gctcctgatc gatgatgccc ttgcgttgca
      361 gtgaccagtc gttgcgttgt attgacataa cttcttatcc gcaaaagtct gctacaactt
      421 tcgtaaaggc ctcgggatac tcctcatgcg gcaggtgccc gcaagcgaga aaaaccacca
      481 gccgggatcc gggtatttcc tgatgaagcc gctcgccgtc ggcgagagga atctcaagat
      541 cgttctcacc ccacaacagc agggttggct gacgaattag atgcgcgtcg ctggagatgc
      601 gttctgcgtt ccagccacgc accgtgcgga tgatcgcgcg ctgcgtaccc gatgcccgca
      661 acggaaaatg gcgcgcatcg acgcgtcttt catccagcac ccacgcgtgc cgatcgtaaa
      721 ctcgcttcat tcgtttgcgc agcagtcgcc gcgaaccgat cagaagcggt gacacaacat
      781 ctccgaccac acgtgaacta aaaatccgca tcaccttgaa ccgcagtggc tcattgttgt
      841 ttaccgttcc caccagcacc agcttctcca cgcgatgcgg atggtccaac gcgcacgttg
      901 cggctactgc gccgccgtac gaactgccaa ccaacgttgc gcgctttata cccaaacgtt
      961 ccaacagtct gatcagcagg cttgcttgtc cggcaatcgt gtactcaccg tttcgtggtt
     1021 ttccggagta accgtaaccc aacaggtcga cggcaattac gcgaaagccg gcctgcgcca
     1081 atttgagaaa caccttgctc cagacgagtg tcgatgaagc gaagccgtga atcaaaacca
     1141 ggaccggatt cgatcggtcg ccggcttcct ggtagtgaac gcgaacgccg tcgatctcca
     1201 cgaagcgcga atgttcggag tgaaaaacga cgtggcgata cttctgccag tctgcgtcac
     1261 gcggtcgcgt cagaagcttg gcagcgactg cgatggtggc agcagccgtt gccgttccgg
     1321 cgatccagta acgtttcttc atcttcatgt ttaagccaca gactacacag acaaacacgg
     1381 accagttttt taggtttgat ccgtggtagc cgtgtaaatc tgtggcctaa ctaacgtgac
     1441 agcaacgtgc cgacgtaggt caacaattcg ttcgcacaga cgggacagta tccatgttcc
     1501 ttcacgaggc ggtcgactac ttcgttgatc ctccgcagtt ggtccggatc cggcgtctta
     1561 gccgaagtcg tgatcttcac gacgtccttt aaatccgcga agatcttctt ctcaatcgct
     1621 tctttgaggc gctcgtggga attgtattca aacggtttgc ctttgcgcgc gtaggacgag
     1681 atccgaatca gtatctcctg ccggaacgtg ttcttggcgt tttcggaaat accgatctgc
     1741 tcttcgatcg agcgcatcaa ctgttcgtcc ggatccatct cctcttcggt aatcggatcc
     1801 ttgatccgtt ccttgttgca gaaggcctca acgttgtcga gatagttatt gcacatcgtg
     1861 cgcgccatct cttcaaacga gtagacaaaa gcacgttgca cttcgatctt cgccaactcg
     1921 tcgtactctt tgcgcgcctg cgagatgtgg ttcaaataac gttcgcgctg ttcggcgctg
     1981 atgccggtgt gctgttcgaa gccgtccttg atcgcgcgca aggcgtcgat cggattgata
     2041 caggtgacgc cgtcgcgaac caacgcgctc gaaagtcgat tgatgatgta gcgcggcgag
     2101 attccgtcca tgccttcgcg gacggtctct tctttcaact cgcgaacgtc tttcgacttg
     2161 taaccctcga cgtcttcacc gtcgtaaagc ttcatctttt tgacgatgtc gacgttttgg
     2221 cgcttgggtt cttcgagacg cgtcattacg gcaaacatcg cggcgacgcg cagcgtgttc
     2281 ggcgccaggt gaacattgcg caaagcttct gactggtg
//
LOCUS       DASTEY010000014         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1170245, whole genome shotgun sequence.
ACCESSION   DASTEY010000014 DASTEY010000000
VERSION     DASTEY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170245"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..297
                     /locus_tag="VFO72_00225"
     CDS             70..297
                     /locus_tag="VFO72_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477735.1"
                     /translation="MYRRPRFLEVLHSIREEMSREADYDVDLFAEMVRSGVRPSHGPQ
                     RVIRGFRSRAPRAESANGKTAKRRQRKRSAQ"
     gene            326..1111
                     /locus_tag="VFO72_00230"
     CDS             326..1111
                     /locus_tag="VFO72_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HET9477736.1"
                     /translation="MNPTFETIGLEVSSEKAFNYLAERAGDQGEATQLSRRGAVLHGR
                     CWKLGEGLEVWTVLYESGSGDVFYADCRPGFRARYIQTIAPWALTEYDEEGEAVIHGF
                     REGTDCEVLFELQNLTEVGPAGFGAQELHIGLCGLAYRARVRRSKAKTRWLPLEKAAP
                     ARAAHENDWSLCGRVIAFKPLTNPLSGGELFWVHLDLERIKLEVLVNRRALRGELAIG
                     ASLEAEVWLQGHVLDEHALRSRYEGVDHAHKCASFWSTLRRKN"
     gene            1167..1739
                     /locus_tag="VFO72_00235"
     CDS             1167..1739
                     /locus_tag="VFO72_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9477737.1"
                     /translation="MKRVLVVVLILLTLVIVAYSVNQYWTHRFDALIARQAAIYRVDP
                     DLVWSIIYEETYFSPWKTGAAGEIGLMQVTPTVGREWARETGMSELERQMASNPHSLL
                     RDPERNIQIGCWYLEKIYDQFQDVPDAETRMVAGYNAGPSRAAEWSRVADGAPPQSGA
                     EFIQRIDIPSTRAYVISIMERYRQLKNAKR"
     gene            1736..>2136
                     /locus_tag="VFO72_00240"
     CDS             1736..>2136
                     /locus_tag="VFO72_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9477738.1"
                     /translation="MTTIYSARWILPALSAPIDYGAVAVDGPLIVGVGDAAEIASRFP
                     AARVEDFGQSVILPGLINTHTHLELTALRGYLENEESDFFAWLRKLTLARLERMTADD
                     IRVSATWGACEAVRAGITCVGDASDSAMMSM"
BASE COUNT          452 a          553 c          662 g          469 t
ORIGIN      
        1 aattttgatt gttgattgaa aaagcaaatc ggcaatcggc aattggcaat cggcaatcga
       61 aaatgccata tgtatcgtcg cccaagattt ctggaagtgc tgcattcgat tcgcgaagag
      121 atgtcgcgcg aggctgacta cgatgtcgac ctgtttgcag agatggttcg atccggtgtg
      181 cgcccgtcgc atggaccaca gcgcgtgatt cgcggtttcc gcagtcgcgc accgcgggca
      241 gaaagcgcca acggcaagac ggcgaagcgt agacagcgga agcgtagcgc gcaataatgc
      301 agtcagccag aagctactga tcaatatgaa ccctactttc gaaacgatcg gtttggaagt
      361 ttcaagcgag aaggctttca actaccttgc cgagcgagcc ggcgatcagg gcgaagcgac
      421 gcaactcagc cgtcgcggcg ctgtgctaca tggccggtgc tggaaactgg gtgaaggtct
      481 agaagtttgg accgtgcttt atgagtcggg cagcggcgat gtgttctacg ctgattgccg
      541 tcccggtttc cgcgcgcgct acattcagac gatcgctccg tgggctttga ctgaatacga
      601 cgaagagggc gaagcagtca tacacggttt tcgcgaaggc accgattgcg aagttctttt
      661 tgaattgcaa aatctgacgg aagttggacc agcagggttt ggcgcgcagg agttgcacat
      721 cggtctgtgt ggtttggctt atcgcgcgcg tgtgcgacgt agcaaggcaa agacgcgctg
      781 gttgccgctc gagaaggccg ctccggcccg cgcggcgcat gaaaacgact ggagtttgtg
      841 tggccgcgtc atcgctttca agccgctgac aaatccgctt tccggcggcg aactgttctg
      901 ggttcacctc gatctcgaac gtatcaaact cgaagtgctg gttaaccggc gcgcgcttcg
      961 cggcgaactg gcgattggcg cgagtctgga agcggaagtt tggctgcagg gacacgtact
     1021 cgacgagcac gcgctgcgct cgcgttacga aggcgtggac cacgcccaca agtgtgccag
     1081 tttctggtct accttgagaa gaaagaacta aagccatcca aacattacac agactccgtg
     1141 taatctgtgg atggttttgc tactggatga aacgcgttct tgtcgtcgtt cttatactcc
     1201 tcacactagt catcgttgcc tactctgtca atcaatactg gacccatcgt ttcgacgcgc
     1261 taatcgctcg ccaggcagcc atctatcgcg tggatccgga tctggtgtgg agcatcattt
     1321 acgaagagac ttacttcagc ccgtggaaga ccggcgccgc cggcgagatc ggtttgatgc
     1381 aggtgacgcc cacggtcgga cgcgagtggg caagagaaac aggcatgagc gaactcgaac
     1441 ggcagatggc gagcaatccg cacagcctgc tgcgcgatcc ggaacgaaac attcagattg
     1501 gttgctggta tctcgagaag atctacgatc agtttcagga cgtacctgat gccgagacgc
     1561 gcatggtcgc gggttacaat gctggaccga gtcgcgccgc tgagtggagc cgggttgcag
     1621 atggcgcgcc gccgcagagc ggggcggagt tcattcagcg gatcgatatt ccttcgacgc
     1681 gcgcgtatgt gatctcgatt atggagcgtt atcgccagct caagaacgcg aagagatgac
     1741 cacgatttat tccgcgcgtt ggatcctgcc agctttgtcg gcgccgatcg actacggcgc
     1801 ggttgccgtc gatggaccat tgattgttgg tgttggcgac gcggctgaga ttgcctcgcg
     1861 atttcctgct gcgcgggtag aagacttcgg gcagtcggtc atccttcctg gtctcatcaa
     1921 cacgcacaca catctcgaac tcacggcgct gcgtggttat ctcgaaaacg aagagagtga
     1981 tttttttgcg tggctcagga agctgacgct ggcgcgcctc gagcgcatga ccgcggatga
     2041 cattcgtgtg tccgcgacgt ggggcgcgtg tgaagcagtg cgtgccggca ttacatgtgt
     2101 gggtgatgcg agtgactccg cgatgatgag catgtt
//
LOCUS       DASTEY010000015         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_362759, whole genome shotgun sequence.
ACCESSION   DASTEY010000015 DASTEY010000000
VERSION     DASTEY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362759"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFO72_00245"
     CDS             <1..715
                     /locus_tag="VFO72_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9477739.1"
                     /translation="KMTESDFDREFNAYVESKVRPFQQALSTESNVVASLSKEEVVKQ
                     LETRDTFALRIRAAELFAADGDTATAATHYIRALELFPYVTGRGNPYESLARLLEQKG
                     DQAQAAAILEKLVSTDENNLEALKTVARIRSALGQRQQALDALLASFYISPFDYNLHK
                     QAGELSVELKNYPQALAEFQVALALQPPNIAEANYNVAMAFHALGQQPEAKRAVLRAL
                     EAAPRYEKAQELLLRIVGQ"
     gene            747..1766
                     /locus_tag="VFO72_00250"
     CDS             747..1766
                     /locus_tag="VFO72_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9477740.1"
                     /translation="MAVDQLRISDEQLLDKLGSARERLLREIRKAIIGQDEVVEQVLL
                     ALFVGGHSIITGVPGLAKTLLVRTIASVLDLSFKRIQFTPDLMPADITGTEIIEEDRA
                     TGRRALQFIPGPIFANIVLADEINRTPPKTQAALLEAMQEGRVTVQGVSYDLPRPFHV
                     LATQNPIEMEGTYPLPEAQLDRFMFNVKIGYLPEEEEVAVVKQTTSPQDYEFERLMTA
                     DEIIAFQRLVRQVPASDTVTRYAVRLVNASRPNTPPAPDFVNKWVTWGASLRASQFLI
                     FGGKARAILRGRYNVSCEDVRAVAPAVLRHRVLLNFQAEAEKVDSDQVIKKLIETVPE
                     PKSGL"
     gene            1763..>2337
                     /locus_tag="VFO72_00255"
     CDS             1763..>2337
                     /locus_tag="VFO72_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9477741.1"
                     /translation="MNHRETQTTQTPEAITPARFLDPELLARIGSLELLARAVVEGFM
                     SGLHRSPFTGFSTEFTEYRQYNPGDDLRYLDWRLLGRTDRYFIKKYRADTNTQCHLLI
                     DTSASMNYAHASSVTKLDYAKFLAASLAYLLNRQQDAVGVVAFDQKVHTHVPARNRTG
                     HMRTIFGNLSLLEPGGETRLAESLHQLAEILT"
BASE COUNT          531 a          618 c          703 g          485 t
ORIGIN      
        1 gaaaatgacc gagagcgatt tcgatcgcga gtttaatgcg tacgttgagt cgaaggtgcg
       61 gccgtttcaa caggccttga gcacggaaag taatgtggtt gcgtcgctct cgaaagaaga
      121 agtggtgaaa caactcgaga cacgcgatac gttcgcgctt cgcattcggg cggcggagtt
      181 gtttgccgcg gacggcgaca cagcgaccgc agcgacccac tacattcgcg cactcgaact
      241 gttcccgtac gtcaccggaa gaggaaatcc ttacgagtcg ctggcgaggc tgctcgaaca
      301 gaagggcgat caggcacaag ccgccgcgat cctggaaaaa ctggtgagta cagacgagaa
      361 caatcttgaa gcgttaaaaa ccgtggcgcg cattcgatcc gcgttgggcc agcggcaaca
      421 ggcgctcgac gcattgctcg cgagttttta catcagtccg tttgactata acctgcacaa
      481 acaggcgggt gagctgagtg tcgagttaaa aaactatccg caggcgctgg ccgagtttca
      541 ggttgcgctg gcacttcagc caccgaatat cgccgaagca aactacaatg tagccatggc
      601 ctttcacgcg ctggggcagc agccggaggc caaacgcgcc gtgctgcgcg cactcgaggc
      661 cgcgccgcgt tatgaaaaag cgcaagaact gttactacga attgttgggc agtgaatctg
      721 tgtaatctgt agataaacga ggaaacatgg cggtcgatca attaagaatc agcgacgagc
      781 aactgctcga taaactcggc tcagcgcgtg agcgtctcct gcgcgaaatc agaaaggcaa
      841 taatcggaca ggacgaagtc gtggaacagg tgctgctcgc gctctttgtt ggcgggcact
      901 cgatcatcac cggtgtgccc ggcctcgcca aaacgcttct cgtacgcacg atcgccagtg
      961 ttcttgatct ctcgttcaaa cgtattcagt tcactcccga cctgatgccg gctgacatca
     1021 ccggcacgga aatcatcgaa gaagatcgcg cgacgggacg ccgcgcgctc cagtttattc
     1081 ctggtccaat ttttgcgaac atcgtgctcg ccgacgagat aaatcgcacg ccgccaaaaa
     1141 cgcaggcggc gctgttggaa gcgatgcagg aaggacgcgt cacggtccag ggcgtcagct
     1201 acgatctgcc gcgaccgttt catgttctgg cgacacagaa cccgatcgaa atggaaggca
     1261 cctatccgct gccggaagcg caactcgatc gtttcatgtt caatgtgaag attggttact
     1321 tgcctgagga ggaagaggtc gccgtcgtca aacagactac ttcgccacag gattacgagt
     1381 ttgaacgttt gatgacggcc gacgagatca tcgcttttca acgactcgtg cggcaggtgc
     1441 cggcgtcaga cacggttacg cgttatgcag tccgtttggt gaacgcgagc agaccgaata
     1501 cgccacctgc gccggacttt gtgaacaagt gggtgacgtg gggcgcgtct ctgcgcgcgt
     1561 cgcagttctt gatctttggt ggcaaggcgc gcgcgatact gcgtggccgt tataacgttt
     1621 cgtgcgaaga cgtgcgcgcg gtggcgccgg cggtcttgcg gcatcgcgtg ttgttgaatt
     1681 tccaggctga agccgagaag gtcgattcag atcaggttat taagaaattg attgaaactg
     1741 ttccggagcc gaagagtggg ttgtgaacca cagagaaaca cagacaacac agacgcctga
     1801 ggcaatcaca ccggcgaggt ttctcgatcc ggagttgctg gcgcgcatcg gatcgctcga
     1861 gttgctcgcg cgtgcggtgg tcgaagggtt catgtcaggt ttgcatcgct cgccgtttac
     1921 ggggttttcg acggagttca cagaataccg gcaatacaat cccggcgacg atttgcgcta
     1981 tctcgactgg cgactgttgg ggcggacgga tcgttatttc ataaagaagt atcgtgcgga
     2041 cacgaatacg cagtgtcacc tgttgattga taccagtgct tcgatgaact acgcgcacgc
     2101 gagttcggtg acgaagcttg actatgcgaa atttctcgcg gcgtcgctgg cgtacttact
     2161 gaaccgccag caggacgccg ttggcgtggt ggcgttcgat caaaaggtgc acacgcatgt
     2221 gcctgcgcgc aaccgtacgg gccacatgcg cacgatcttc ggcaatctgt cgttgttgga
     2281 gcccggcggc gaaacgcggc tggcggaatc gttacaccaa ctcgcggaga ttctgac
//
LOCUS       DASTEY010000016         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_428686, whole genome shotgun sequence.
ACCESSION   DASTEY010000016 DASTEY010000000
VERSION     DASTEY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428686"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFO72_00260"
     CDS             complement(<1..364)
                     /locus_tag="VFO72_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9477742.1"
                     /translation="MTNSALSRRSFSKLIGASAAYAAFRPVEVNASWPAANIVRLSSN
                     ENPYGPSPAALKAMTDGFSLAWRYPDEYADMLADELARLHGVPLDQVLLGDGSGEILK
                     LCAAAFTSKDKKIVIANPT"
     gene            complement(374..2185)
                     /locus_tag="VFO72_00265"
     CDS             complement(374..2185)
                     /locus_tag="VFO72_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET9477743.1"
                     /translation="MAKILILAILVFVPCVRAQQTDLIANTDGRKTISLDGKWQVIVD
                     PYESGYYNYRYQPSDNGYFKNAKPKTKSDLIEYDFDSSESLQVPGDWNTQSDQLFFYE
                     GTIWYKKSFDYQRRPNTRLFVYFGAANYLADVYLNGEKVGQHEGGFTPFNFEITKLVR
                     DSGNFLVVKVDNKRRRDAVPTLMTDWWNYGGLTREVKLIETSTTFVHDYMIQLAKGSR
                     NRVSGWVKLSGDKLKQRITIRLPEAGISKSFATDANGFAAIDFNADLKLWSPENPKLY
                     DVAIEAETDQVRDQIGFRSLETKGTDILLNGRSIFLRGICIHEEAPLRGGRAYSREDA
                     LTLLGWAKELGANFVRLAHYPHNEFMVREADRMGIMVWSEIPVYWTIQWENPSTLENA
                     RNQLSEMIARDKNRAAVVIWSVANETPLSDARLSFLKNLISHARSLDATRLLSAAMER
                     HYIDATTQMIDDPLGEYLDVLGCNEYVGWYDGLPEKADRLEWKSKYQKPLVMSEFGGD
                     ALYNHHGDPLMRWTEEYQENLYRHQLAMLKKIPFLRGTSPWILTDFRSPRRPLPGIQD
                     YWNRKGLISDRGEKKKAFYVMQEWYRELQTKEQPAPK"
     gene            complement(2213..>2972)
                     /locus_tag="VFO72_00270"
     CDS             complement(2213..>2972)
                     /locus_tag="VFO72_00270"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9477744.1"
                     /translation="GTPFEAFAVKNGVDQTATEGVSDTHYNIPNLHVSVHHPKVNVPV
                     LWWRSVGHTHTAFVMETLIDELATRAKMDPIAYRRRLLKPEAKKLLRPLDLLEKASAG
                     WRNRLPRGHALGVSCHESFGTGVACAVDVSIENKRPKIHRATIAVDCGLAVNPLSVES
                     QFQAGVSFGLSQLMAKGAITLKNGVVEQRNFDGYVPPYIGDAPIAVDVHIVPSTEAPT
                     GCGEPPVPVISPAVVNALARLTGKRYRTLPLVSI"
BASE COUNT          622 a          833 c          821 g          696 t
ORIGIN      
        1 aagtcgggtt agcgatgacg atctttttgt ctttgctggt gaaggcagcc gcgcagagtt
       61 tcagaatctc tcccgagccg tctccgagaa gcacctggtc cagcggtacg ccgtgcagtc
      121 gcgccaactc atcagccagc atgtccgcat actcatcggg atatcgccac gcgagactga
      181 aaccgtcagt cattgctttc aacgccgcag gtgacggtcc ataaggattc tcgttcgaac
      241 tgaggcgaac gatgtttgcg gctggccacg acgcgttgac ttccaccggc cggaatgcag
      301 cataagccgc actcgcacca atcaatttcg agaagctgcg acgagaaagt gctgagttag
      361 tcatcatgcc tcctcatttc ggcgcgggct gttctttagt ctgcaactca cgataccact
      421 cctgcattac gtagaacgcc ttcttcttct cgccgcgatc cgagatcaat cctttgcggt
      481 tccagtaatc ctgaattccg ggtaacgggc gtcgcggaga gcgaaagtca gtcaggatcc
      541 acggagacgt gccgcgcagg aacggaatct tcttcaacat tgcgagttga tgacgataaa
      601 ggttctcctg gtactcctcg gtccatctca tcagcggatc gccgtgatga ttgtagagcg
      661 cgtcaccacc gaattcactc atcaccagcg gcttttgata cttcgacttc cattcgagtc
      721 gatcggcttt ctccggcaga ccatcgtacc agccaacata ctcgttacag ccgagcacgt
      781 cgaggtattc gccgagcggg tcgtcgatca tctgcgtcgt tgcgtcgata tagtgacgct
      841 ccatcgcggc cgacagcagg cgtgtggcgt cgaggctgcg agcgtgcgag atcaggtttt
      901 tgagaaacga aagacgcgca tcgctgagtg gtgtctcatt ggccaccgac cagatcacaa
      961 cggccgcgcg gttcttgtcg cgtgcgatca tttcggagag ttgattgcgg gcgttttcga
     1021 gtgtggaggg gttttcccat tggatggtcc agtagacagg aatctctgac cagaccatga
     1081 tgcccatcct gtcagcctct cgaaccatga attcgttgtg cggatagtgg gccagccgaa
     1141 caaagtttgc gccgagctct ttggcccaac cgagcaaggt cagcgcgtcc tcgcgactgt
     1201 acgcacgccc accacgtaaa ggcgcttcct catgaatgca gataccgcgc agaaagattg
     1261 agcgcccgtt cagcagaatg tcggtgcctt tcgtttcaag actgcggaag ccgatctggt
     1321 cgcgcacctg atccgtttcc gcttcgatcg ccacgtcgta gagcttcggg ttctccggcg
     1381 accatagctt cagatcagcg ttgaaatcga ttgccgcaaa tccgtttgca tccgtggcaa
     1441 atgatttgct gatccccgct tcaggaagtc ggattgtgat tcgttgtttg agtttgtcgc
     1501 ccgacaattt cacccagccg ctgacccgat ttcgcgagcc tttggccagt tggatcatat
     1561 agtcatggac gaaggtggtt gaagtttcaa tcaacttcac ttcgcgagtg agcccaccgt
     1621 agttccacca atcggtcatc aacgtcggaa cagcatcgcg ccggcgtttg ttatcgactt
     1681 tgacgacgag gaagttgccg ctatcgcgaa cgagtttggt gatctcgaag ttaaaaggag
     1741 tgaagccgcc ttcgtgttgt ccgactttct cgccgttgag gtatacgtcc gctaggtagt
     1801 tcgcggcgcc gaagtagacg aagagacgtg tgttgggccg gcgctggtaa tcgaacgact
     1861 tcttgtacca gatggtgcct tcgtagaaga acagttgatc ggattgcgta ttccagtcgc
     1921 ccggcacctg caacgattcg gagctgtcga agtcgtattc gatcaggtcg cttttggttt
     1981 tcggtttggc gtttttgaaa taaccgttgt cgcttggttg atagcggtag ttgtagtaac
     2041 cggattcgta cggatcgacg atcacctgcc acttgccatc gaggctgatg gttttgcgac
     2101 cgtcggtatt tgcgatcaga tcagtttgct gtgcccgcac gcacgggaca aacacaagta
     2161 tcgcaagaat aagtatttta gccacagatg aacgcggaag aagacggatt aactaaatac
     2221 taactagtgg cagcgtgcgg tagcgctttc cggttagtcg cgccagcgcg ttgacgaccg
     2281 cgggcgagat caccggaaca ggtggctcgc cgcagccggt tggcgcctca gtgctcggca
     2341 cgatgtgaac atccaccgcg atgggtgcgt cgccgatgta cggcggaacg taaccatcga
     2401 aattccgctg ctccaccact ccgttcttta acgtgatcgc gcccttggcc atcaactgcg
     2461 acaggccaaa cgacacgccc gcctgaaact ggctctcgac cgacaacgga tttacagcga
     2521 gcccacaatc gactgcaatc gtcgctcgat gaatcttcgg acgcttattc tcgatcgaca
     2581 cgtcgacagc acacgccacg ccagttccga aagactcatg acaggaaact ccgagcgcgt
     2641 gcccgcgtgg caacctgttg cgccaaccag cgctcgcttt ctcgagcagg tcgagtggtc
     2701 gcaacagctt cttcgcttca ggcttgagta gccggcgacg ataggctatc ggatccattt
     2761 tcgcgcgcgt tgctaattcg tcgatcagcg tctccatgac aaacgcggtg tgcgtgtgtc
     2821 cgaccgagcg ccaccagagc acgggcacgt ttaccttcgg atgatgaacc gagacgtgca
     2881 gattcggaat gttgtagtgt gtgtcagaca ctccttctgt cgccgtctgg tccacaccgt
     2941 tcttgaccgc gaacgcttcg aatggagttc cc
//
LOCUS       DASTEY010000017         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1038533, whole genome shotgun sequence.
ACCESSION   DASTEY010000017 DASTEY010000000
VERSION     DASTEY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038533"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFO72_00275"
     CDS             <1..476
                     /locus_tag="VFO72_00275"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET9477745.1"
                     /translation="VAGIDETLIPNEEAAAYVKRLARSKAEAVASRLSTGLVLGADTT
                     VVVANQILGQPADDADARRMLNLLNAKWHEVLTGVAVVRVGGETRVAYETTRVRFAEM
                     NEREIDWYIATGEPFGKAGAYGIQGKASLFIEEIEGDYFNIMGLPIRLVYELAAD"
     gene            complement(508..1272)
                     /locus_tag="VFO72_00280"
     CDS             complement(508..1272)
                     /locus_tag="VFO72_00280"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9477746.1"
                     /translation="MLKGKTALITGGGRGIGRAIALAFAREGARIAVAARTVEQIEQV
                     ATEIGNGSIALTCDVADPESVKRMFGELRERFGHADILVNNAGIAESATLVNTTDEHW
                     HRHLSINLSGTFYCTRAALPAMLKNGWGRVINIASIAGKTGAPYITAYSASKHGVMGL
                     TRSLALEVATTGITVNAICPGYVDTEMVSRGVERITERTGRTAEEALDSLKKMSPQNR
                     LATPEEVAAVALLLASDEGRGINGQGINVDGGSVLF"
     gene            complement(1277..2065)
                     /locus_tag="VFO72_00285"
     CDS             complement(1277..2065)
                     /locus_tag="VFO72_00285"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET9477747.1"
                     /translation="MYEHIQIAEADGITTITLNRPDRLNAFIGHMRRDLAEALEHAGS
                     DRNVRVVIITGAGRGFCAGGDIAFMAELMQRRDTEEFSRILGAGRRVILAIRQMTKPV
                     IASINGPASGAGCNLALACDLRIATNTTTFSQSFAKVGLHPDWGGTYFLPRLVTPNKA
                     CELFFLGDTIDAAEALRLGIVNQVVAPEDLETATLQLAKRLRAAPPIALAAAKQAVYM
                     SQAAELDEMLRFETEAQLRCFESDDGHEGVQAFLEKREPRFTGH"
     gene            complement(2080..>2354)
                     /locus_tag="VFO72_00290"
     CDS             complement(2080..>2354)
                     /locus_tag="VFO72_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9477748.1"
                     /translation="LGILNHVFEAETIENFAEQVHDYARQFTTPNKAAHAVGRIKRAV
                     QTGAEISFEPALAFERELQQQLFQSEDAKEGLDAYVNKRKAEFKGR"
BASE COUNT          493 a          641 c          679 g          541 t
ORIGIN      
        1 tcgtcgcagg catcgacgag acgctgatcc cgaacgaaga agcagcggcg tatgtaaagc
       61 ggcttgcgcg ttcgaaagcg gaagccgtcg cgagtcgatt aagcaccggt ttggtgttgg
      121 gtgctgatac gactgtagtc gtcgcaaacc agatcctggg ccaaccggct gacgatgcgg
      181 atgcgagaag gatgttgaac ttgctgaatg cgaagtggca cgaggttctg actggtgttg
      241 ctgttgttag agtcggtggc gaaacacgag ttgcttacga aacgacgcga gtgcgttttg
      301 cggaaatgaa tgagcgtgaa atcgactggt atatcgctac cggtgagccg tttggcaaag
      361 caggagccta tggaatacaa ggaaaggcgt cgctcttcat cgaagagatt gaaggcgact
      421 acttcaatat catgggattg cccatcaggc ttgtttacga attggccgcc gattaacgcg
      481 gatcttcgta ctgcagattt tcacggatca gaacaggaca gaaccgccat caacgttgat
      541 gccctgacca ttgatgcctc gcccctcgtc agatgccaat aacaatgcta cagcagcaac
      601 ttcctcggga gttgcgaggc ggttttgcgg gctcatcttc ttgagtgaat ctaaagcttc
      661 ttcggccgta cgtccggtcc gctctgttat tcgttcaacg ccacgcgaga ccatctcagt
      721 atcgacatag ccgggacaga ttgcgttcac ggtgattccg gtggttgcga cttcgagtgc
      781 gagcgagcga gtcaaaccca tcacgccgtg ctttgaagct gaataagcgg tgatgtaagg
      841 cgcgccggtt ttgccggcaa tggaagcaat attgatcact cggccccagc cgttcttcag
      901 catcgcaggc aatgctgcgc gcgtgcagta gaacgtgcct gacaggttga tcgagaggtg
      961 gcggtgccag tgttcgtcgg ttgtattgac gagcgttgcg ctttcggcaa tgccggcgtt
     1021 attcacgaga atatcggcgt gaccaaaacg ttcgcgcagc tcgccgaaca ttcgcttgac
     1081 gctttccgga tcggcaacgt cgcaggtgag cgcaatggag ccgttgccaa tctcggttgc
     1141 aacctgctcg atctgttcaa ctgttctggc ggcgacagcg atgcgcgctc cttcgcgtgc
     1201 gaacgcgagc gcgattgcgc gtccgatgcc tcgtccgccg ccggttatga gtgccgtctt
     1261 tcctttgagc ataatattag tgaccagtga accggggctc gcgtttttcg agaaatgcct
     1321 gtaccccctc gtgaccgtcg tcagactcaa aacaacgcaa ctgagcttct gtctcgaagc
     1381 gaagcatctc gtccagctct gccgcctgac tcatgtagac cgcttgcttt gccgcagcca
     1441 gtgcgattgg tggcgctgcg cgcaggcgtt tggcgagttg caatgtggca gtctccagat
     1501 cttccggcgc tacaacttga ttgacgatgc ctaatcgtaa agcttccgcc gcatcgatcg
     1561 tatccccgag aaagaatagc tcgcacgctt tgtttggcgt aaccaggcgc ggcagaaagt
     1621 aagtgccacc ccagtccgga tgaagtccaa ctttcgcaaa cgattgacta aatgtcgtcg
     1681 tgtttgtggc gatgcgtaag tcacaagcaa gcgcgagatt gcagccggca ccggacgcgg
     1741 gaccattaat cgaggcgatg acaggcttgg tcatctggcg aatcgcgaga atcacgcgac
     1801 gccccgcgcc gagaatgcgt gagaactctt ccgtatcgcg acgctgcatc aactccgcca
     1861 taaacgcgat gtcgccgccc gcgcagaacc ctcgccccgc gccggtgatg atgacgacac
     1921 gcacattgcg atcgctgccg gcgtgctcga gtgcttcggc aagatcgcgt cgcatgtgac
     1981 cgatgaacgc gttcaggcga tccggacgat tgagcgtgat cgtcgtgatg ccgtcagctt
     2041 ccgcgatttg gatgtgctcg tacatattcc gtcttgaacc tagcgtccct taaactccgc
     2101 tttgcgcttg ttcacataag catccaaccc ttccttcgca tcttctgatt ggaaaagttg
     2161 ttgctgtagt tcgcgttcga acgcgagcgc gggctcgaat gagatctccg cgccagtttg
     2221 gaccgcacgc ttgatgcgac cgaccgcgtg cgcggctttg tttggcgtcg tgaactgacg
     2281 cgcataatcg tgcacctgtt cggcgaagtt ctcaattgtt tcagcttcga agacgtgatt
     2341 cagaatccca agct
//
LOCUS       DASTEY010000018         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_263967, whole genome shotgun sequence.
ACCESSION   DASTEY010000018 DASTEY010000000
VERSION     DASTEY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263967"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1040
                     /locus_tag="VFO72_00295"
     CDS             234..1040
                     /locus_tag="VFO72_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477749.1"
                     /translation="MQLRLTKTLTNNKFGTGQIQGVYSWSHSIDDSADGLVPGAGPGG
                     GSERGLPRDSSGFAGGFSLPERGNSGFDVRHRFVLNFIYDLPIRFDNRALDRFLGGWT
                     MSGIVQSQTGTPYSIFGGTDSAGTGLGQRADFAPPGAGLSDLAAATNNPRTYTGPARS
                     LFRNPCPANRTNTSPTACSAAVGPAVGRQGTVGRNQFYGPAFNKVDFSLIKRFPITEN
                     TRFTVRADFFNIFNTVNFGLPANDINSALFGQSTNAGAARIIQFAGRFDF"
     gene            1275..1418
                     /locus_tag="VFO72_00300"
     CDS             1275..1418
                     /locus_tag="VFO72_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477750.1"
                     /translation="MILPHTYKIRLVFESDANPAMQDWELTLSQFLFNKDLPASQFRV
                     TTE"
     gene            1436..2761
                     /locus_tag="VFO72_00305"
     CDS             1436..2761
                     /locus_tag="VFO72_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9477751.1"
                     /translation="MPLRFVFTKFFYFLAALAFVPLALSWQRPWLAWIALAYDFVLIC
                     VAIADVRLSRLPKGVQVTRRFSGRFAMGAETEVRIVIHNPSSRSVLLRIKDEYPPQMI
                     LTGEREGEVQVAAHSTATMIYSLTPPRRGRFEFGYTALRFRSLLNLVWCDARGSEPVA
                     VKVYPNMRRAREAELKALGARSLVSSHRKTSWRGEGREFESMRDYVRGDELRHISWTT
                     TARRGKLTTRQYQIERDQTILVALDSGRLMTARIEQETKLDSAVHATLALFSAAARAG
                     DNAGLIVFGRRIKSYLPPGRGRDHIDAALEALYAVEPEMIEPSYPHAFEFIAANSKRR
                     SLVILLTDLVDEEGSKELLTSLNLLRPRHLPLVVTIADRDLKAVVQGVPTTVRDLFTQ
                     SVSEEIIHHREAALRMVESVGGLALDVTAAALAPALLETYMRVKERGLL"
     gene            complement(2753..3730)
                     /locus_tag="VFO72_00310"
     CDS             complement(2753..3730)
                     /locus_tag="VFO72_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET9477752.1"
                     /translation="MPSDRFISQRKNAWQRLEDLLKLLDHASLRRLGREEVRELGRIY
                     RRTASDLAIARAESRDPRLVNYLNSLVIRAHGRIYRADAQGGKRILRYFTQELPQTFR
                     RTWRYTFLAFFVFLLFAVLSFVGTKYDPEFSELVGVSPAFRELFIETKTHWWEALNKS
                     NQEGASMIMTNNIQVTIYTFAFGAMLGLGTLFYLAFNGANIASVLALTYNAGFGNDLV
                     TFMVAHGVIELSCIFIAGGAGLLIGSAMIMPGNLTRADALRTRGMEAVRLMIGVAMLL
                     VVAGIIEGFVSPAPIDPRIKYSIAAITGLALYGYLLFVGHEPSKEQQSK"
BASE COUNT          874 a         1054 c         1055 g          879 t
ORIGIN      
        1 gattgaacca actgcgcaat atcagtggtt ctttgccgag cgtggtgcga cgaaacgcca
       61 tctgtgcagt gaatccaacg atttcaggct gcggcactca ggctggtgga tctgcagctc
      121 ctacgcagat cagtccgacc agtacccgca caaatttctt gaacggcgta ctgaatacag
      181 ccttcaatcc aatcttcttg aacgtggctc ttggacaatc gaccttcaat tctatgcaat
      241 tgagactgac caagactctt accaacaaca agttcggtac aggccaaatc cagggtgtgt
      301 actcgtggag ccattcaatc gacgactcgg ctgatggttt ggttcccgga gcaggacccg
      361 gtggcggcag cgaacgcgga ctaccccgag actcctcagg attcgccggc ggtttctctt
      421 tgccggaaag aggcaactca ggcttcgacg ttcggcaccg gttcgtcctg aactttatct
      481 acgacctgcc catccgtttt gacaacaggg ccctggaccg ctttctcggc ggttggacca
      541 tgtccggtat tgttcaatca cagacgggca cgccgtatag catctttggt ggtaccgata
      601 gcgccggaac cgggcttggt caacgcgctg atttcgcgcc tccgggcgct ggcctgagcg
      661 atctcgcggc ggctactaac aacccgcgaa cgtacaccgg acccgctaga agtctgttcc
      721 gaaacccgtg tcctgcaaac cgaaccaaca ccagtcctac ggcgtgcagt gctgcggttg
      781 gtccggcagt cggacggcag ggaacagtcg gtcgcaacca gttttacggc ccggccttca
      841 ataaagtgga cttctcactg atcaagcgtt tcccgatcac tgagaatacc cggttcacgg
      901 tccgagccga cttcttcaac atcttcaata ctgtgaactt tggtttgcct gccaacgaca
      961 tcaacagcgc gttgtttggt caatcaacca acgcaggtgc ggcgaggatt atccagtttg
     1021 ccggacgatt cgatttctga gtccggtgct gctaattgag tgttaactta tgggcgttgc
     1081 ttccacggaa acaacgcccg cttttttcct gaagggttat gacaacctgc acccatcgtc
     1141 taatccaatc agctttcgtt ttgcttttag tggtgttggc agcgggtgat tcgctttgcc
     1201 aaaaacgcac ggctgaagac gttgtcaatt taaagctgac ggaagagttc gcagattata
     1261 ggaaagaggg tcagttgatt cttcctcaca cttacaaaat acgactcgta ttcgaaagcg
     1321 atgcgaatcc cgcaatgcag gattgggagt tgacgctaag ccaattcctc tttaacaaag
     1381 atctgcctgc ctcgcagttt cgcgtgacaa ctgagtgaga taaagatata gaatcttgcc
     1441 gttgcgcttc gttttcacca aattcttcta cttcctggcg gcgctcgctt ttgttccact
     1501 cgctctttcg tggcagcgac catggttggc ctggatcgcg ctcgcgtatg attttgtact
     1561 gatctgcgtc gcgatcgccg atgttcggct cagtcgcctg ccaaaggggg ttcaggtgac
     1621 gcgacgtttc agcggccgat tcgcaatggg cgccgaaacg gaagtcagga tagttattca
     1681 taacccgtcg agccgctccg tcttactgag aatcaaagac gaatatccgc cgcaaatgat
     1741 tctaacgggc gaacgcgagg gcgaggtgca ggtggctgct cacagcacgg ccacgatgat
     1801 ctacagcctc acgccgccgc gacgcgggcg attcgagttt ggttacacgg cgctgcgttt
     1861 tcggtctcta ctcaacctcg tgtggtgcga tgcgcgcggt tcggagccag tcgcggtaaa
     1921 ggtctatcca aacatgcgcc gtgctcgcga agcggagctg aaagcactcg gcgcgcgttc
     1981 ccttgtttcg tctcatcgaa agacgtcctg gcgcggcgag ggtcgcgaat ttgaatcaat
     2041 gcgcgactat gttcgcggcg acgaactacg tcacatctcc tggaccacta ctgcgcgccg
     2101 cggaaaactc actacgcggc agtatcagat cgaacgcgac caaacgatcc tcgtcgcact
     2161 cgactcgggc cgtctgatga ctgcccgaat cgaacaggaa acaaaactag attccgcggt
     2221 gcatgcgaca ctcgcgttgt tttctgcggc cgcacgcgcc ggcgataacg ccggtctgat
     2281 cgtgtttggg cgcaggatca aaagttatct gccgccgggt cgtggccgcg atcacattga
     2341 tgcagcgctc gaagcactgt acgcagtcga gccggaaatg atcgaacctt cgtatccaca
     2401 cgcgttcgaa ttcatcgctg cgaacagcaa acgacgatcg ctagttatcc tgctcactga
     2461 tctggtagac gaagaagggt ctaaagaatt gttgacgtcc ttgaatctac ttcgtccgcg
     2521 tcatttgcct ttggtggtga cgattgccga ccgcgatttg aaagccgtag tgcagggagt
     2581 tccaacgacg gttcgcgatc tgtttacgca gtctgtttcg gaagagatca tccaccatcg
     2641 tgaggctgcg ctgcggatgg ttgagtcggt gggtgggctg gcgctcgacg tgaccgccgc
     2701 cgccttagca ccggcgctgt tggaaaccta catgcgcgtg aaggaacgcg gcctactttg
     2761 attgttgttc tttggacggc tcgtgaccaa caaagagtag atagccgtac agcgcgagac
     2821 cggtaattgc ggcgatactg tatttgatcc gcggatcgat cggcgcgggc gaaacaaatc
     2881 cctcaatgat tccggcaacc accagcaaca tcgcaacgcc tatcatcaag cgaacagctt
     2941 ccatgccacg cgtgcgcaac gcgtcggccc gcgttaagtt ccccggcatg atcatcgcgc
     3001 tgccgatcag caaccctgcg ccgccggcaa taaaaatgca cgacagttca ataactccgt
     3061 gcgcgaccat gaacgtaaca agatcgttgc cgaaaccggc gttgtaagtc agcgccagca
     3121 cggaagcgat gttggcaccg ttgaaagcga gatagaaaag agtaccgagt ccgagcatcg
     3181 cgccgaacgc aaaggtgtag atcgtgacct ggatgttgtt agtcatgatc atactggcgc
     3241 cttcctggtt cgatttgttg agcgcttccc accagtgagt ctttgtttcg ataaacaatt
     3301 ctcgaaacgc gggactcacg ccgacaagtt ctgaaaattc gggatcgtac ttggtgccga
     3361 caaaactcag cacggcgaat aagaggaaga caaaaaacgc aaggaacgtg tagcgccacg
     3421 tgcggcgaaa ggtttgtggc agttcctgcg taaagtaacg aagaattcgt ttgccgccct
     3481 gcgcgtcagc gcggtagatg cgtccatgag cgcggatgac aaggctgttt agataattga
     3541 caaggcgcgg atctctcgac tccgcacgtg caatcgcgag gtcagaagcg gtgcgccgat
     3601 agatgcggcc gagttcgcga acctcctcgc gccccaagcg tctaagcgat gcgtgatcga
     3661 gtagtttcaa caaatcctcg agccgctgcc aggcgttttt gcgttgactg atgaacctgt
     3721 cggaaggcat tttgtcggta gtgtacttgg caaagctcca agctcaaaga acaaagtaca
     3781 atccacagat tacacagact attttgtaga atctgtgtaa tctgtggtga agatgcgtca
     3841 attggtcgaa tgtgttccta ac
//
LOCUS       DASTEY010000019         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1598886, whole genome shotgun sequence.
ACCESSION   DASTEY010000019 DASTEY010000000
VERSION     DASTEY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598886"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFO72_00315"
     CDS             <1..786
                     /locus_tag="VFO72_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477753.1"
                     /translation="ATNLVGQRFIFRRGFTRWLMHFLIMWGCVISALITFPLVFGWVH
                     FRLEGDLGYRAYVFGFPLNVMQGRSLIAWITFHALDFTAIMVIAGCAIAIHRRLRDRG
                     AISLQQFTLDFVPHMLLIAISVTGLMLTASSLWLNGYMYHFIALTHQATVIMTLFYLP
                     FGKLFHVVQRPASIGVELYQERARDMAQAKCARCGVEFVSQLWLDDLKTVVDELGFDY
                     SLGDGNTLQDYCPRCKRIMRGLAYATASQKGEQVFVGSRGSQE"
     gene            787..>2267
                     /locus_tag="VFO72_00320"
     CDS             787..>2267
                     /locus_tag="VFO72_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9477754.1"
                     /translation="MGKVGNLESIVERYGPHLHDAPYDGWRADRVIDKVVPTHCPYCA
                     VQCGMNLLVERDHVVGFEPRYDFPVNEGRLCPKGVTAYLQVHHPDRLLFPLIKRDGNF
                     ERASWDEAMELIVTKFKELQEKHGKDSIGVYSGSSLTTEKTYLMGKFARAGLGTRYVD
                     YNGRLCMVSAAAGNNKAFGIDRAANPWSDIPLAEVLLIAGSNCAETFPVLNKFLWQQR
                     DNGGRWIIVDPRETPTARQGDLHLQLKPGTDVALANGLLHVLIEESLTDEAFINSRTN
                     DWEATREMALRFPPAVAAEISGVPVEKIVQAARLYGRAKTEMVMHARGIEHHTNGFNN
                     VLSYINLVLATGTIGAPGRGYGTITGQGNGQGGREHGQKADQLPGQRSIANPEHRKHV
                     CNVWGLPEEELPQAGVSVVEMFDKMREGEIRGLLSICNNVMVSLPDTNKVRESLEGLD
                     FYVCIDFFMSEGARYADVVLPGSAWSEDEGVTCNSEGRVVKIN"
BASE COUNT          500 a          597 c          658 g          512 t
ORIGIN      
        1 gctactaatc tcgtcgggca acgcttcatc ttcaggcgtg gcttcacccg ttggctgatg
       61 catttcttga tcatgtgggg atgcgtcatc tccgcactca tcacgtttcc cctggttttt
      121 ggctgggtcc atttcaggtt ggaaggcgac ctgggctatc gcgcttacgt cttcgggttt
      181 cccttgaacg taatgcaagg gcggagcctg atcgcgtgga tcacctttca cgcacttgat
      241 ttcaccgcca tcatggtgat cgcgggttgc gcgatagcga ttcatcgtcg cctgcgtgat
      301 cgcggcgcaa tctccttaca gcagttcacg ctcgactttg tgccccacat gctgctcatc
      361 gcgatctcgg ttaccggctt gatgttgacc gcatccagtc tttggctgaa cggctacatg
      421 taccacttca tcgcgctgac acatcaagct acagtcatca tgacgctctt ttatctgcct
      481 ttcgggaaac tcttccacgt cgtacagcgg ccagcgtcaa tcggagttga gttgtaccag
      541 gagcgcgcga gagacatggc acaagcgaaa tgcgcgaggt gtggcgtcga gtttgtttcg
      601 caactgtggc ttgatgattt gaagacagtt gttgacgagc tcgggtttga ctactcgctc
      661 ggcgatggca acacgttgca ggattactgt ccgcgctgca aacgaatcat gcgcgggctg
      721 gcgtatgcaa cggcgtcgca aaaaggtgaa caagtatttg tgggatcgag aggttctcag
      781 gaatagatgg gaaaagtagg caatctcgaa agcattgtcg agcgttatgg tccgcatctg
      841 cacgacgcgc cgtatgatgg gtggcgcgcg gatcgcgtca tcgataaggt tgtgcccacg
      901 cattgcccgt attgcgcagt gcagtgcggc atgaatcttc tggtcgaacg agaccacgtc
      961 gtcggatttg aaccacgcta cgactttcct gtcaatgaag ggcgtctctg tccgaagggc
     1021 gttacggctt acctgcaagt ccatcacccc gatcgcctgc tgttcccttt aatcaaacgc
     1081 gatggcaatt tcgagcgcgc aagttgggac gaggcgatgg agctgatcgt tactaagttt
     1141 aaggaactcc aggaaaaaca cgggaaggac tcgatcggtg tctattcggg atcgtcgctc
     1201 accaccgaga agacttatct gatgggtaag ttcgcacgcg ccggactggg aacgcgttac
     1261 gtcgattaca acgggcgcct ctgcatggtc tcggcggcgg cgggaaataa caaagccttc
     1321 ggtatcgatc gggccgcgaa tccctggagc gatattccgc tggcggaagt tttgctgatc
     1381 gccggatcga attgcgccga gacctttcct gtacttaaca aatttttgtg gcagcaacgc
     1441 gataacggcg ggcggtggat catcgtcgat ccgagagaaa caccgacggc cagacaggga
     1501 gacttgcact tgcagctcaa gcctggcact gacgtcgcgc ttgcgaacgg cctgctccac
     1561 gtcttgattg aagagagcct gactgacgaa gcgttcatta acagtcgcac aaacgactgg
     1621 gaagcaacac gcgaaatggc cctgcgattt ccacctgctg ttgctgctga aatcagtggt
     1681 gttccagtcg aaaagattgt tcaagctgcg cgactttatg gtcgagccaa aacagagatg
     1741 gtcatgcacg cgcgcggcat cgaacatcac accaatggct tcaacaacgt gctgtcgtat
     1801 atcaacctcg tgttagcgac cggaacgatc ggagctccgg gtcgcggcta tgggaccata
     1861 accggacaag gcaacggcca gggcggacga gaacacggac aaaaagcgga tcagcttccg
     1921 ggtcagcgtt cgatcgcgaa tccggaacat cgcaagcacg tttgcaacgt gtggggattg
     1981 ccggaagaag aactgccgca ggccggtgtc tcggttgtcg agatgttcga caagatgcgc
     2041 gaaggtgaga tccgaggact gctctccatc tgtaataacg tcatggtcag tctgcccgac
     2101 acgaataaag tgcgcgagtc gcttgaagga ctcgacttct acgtttgtat cgacttcttc
     2161 atgtcggagg gcgcgcgcta cgccgacgtg gtcctaccag gctcggcctg gagtgaagac
     2221 gaaggcgtga cctgcaacag cgaaggccgc gtcgtcaaaa ttaataa
//
LOCUS       DASTEY010000020         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1137455, whole genome shotgun sequence.
ACCESSION   DASTEY010000020 DASTEY010000000
VERSION     DASTEY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137455"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VFO72_00325"
     CDS             <1..569
                     /locus_tag="VFO72_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477755.1"
                     /translation="HDRIGEVRPWLVAGEKYQAEDEDLFGLVVRGLAILDEIEAARKL
                     AASEPSRMKTSAVANLSLGKALSSHEQYVEAERLLNTAAQLDKKSTNAHIALAVLYRK
                     KSRWLAALNAANKAVSLDAEDSEGYYQKACVLARLGRIKEAMAALTKSFELDEEQTMY
                     IAEEDDLKPLASLPAFKKLLPAPEKQQP"
     gene            699..1181
                     /locus_tag="VFO72_00330"
     CDS             699..1181
                     /locus_tag="VFO72_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477756.1"
                     /translation="MTHKSLPLFLVLVLFSTHAPLALAQQSVSTNNWTAVQQLKTNER
                     LVVKQKNGKEVKGRMIEATDTTLTIDRDGKPVAIARDDVRRVSVIERKAEKGKYALIG
                     AGIGAGTGTAIGYAAYDPHGDNEVYILMGSLIGVGTGALGGFLFGLGKRKRVMVYAVD
                     "
     gene            complement(1475..1708)
                     /locus_tag="VFO72_00335"
     CDS             complement(1475..1708)
                     /locus_tag="VFO72_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HET9477757.1"
                     /translation="MSSPLPVPPSGFDALSRAEQLDYIEQLLNYVQSGAKYVDIPEWH
                     REQLTELMARYREVGFEGTTWEDFQQELEQEST"
     gene            1926..4217
                     /locus_tag="VFO72_00340"
     CDS             1926..4217
                     /locus_tag="VFO72_00340"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET9477758.1"
                     /translation="MVRFLFGHEQAVFTNGRFGFDVRPGALLLVIIFLLAAAFIYFIY
                     LRPRIKLSKRTTATLIALRAALLTMMIVLLLRPVVVVSSVIPRSSYVAVLVDDSLSMK
                     LSDVAGGATRLDTVKQALLNTTAGQTSFLNRLDQKFKTNLYGFSGVLSRLKDGNDLFG
                     EGRTSNLAGALEETIKRSSGMPLSAIVLATDGASNVPSDITSTLRELRARDIPVFAIG
                     VGATSKSLDAELTRVNMPRRVLVGSRVNIEAFVGLSGYETTKVLLGVREDGRAIKTEQ
                     FDLRGNDTQAINLEITPTTPGLHRYTVEITPLDGELTVENNKQDALVEVIEGPLRLLH
                     VEGEPRWELGKIRESLALNEKNVVLVSLQRTGENKFYRQGISNQAELATGFPETEEEL
                     FSYDGLVVGSVEAGFFSADQLRSIEAFVARRGGGLLALGGRLSFDGGKYKGTTIDDLL
                     PVSLTGGPVDDVNSFAPVYRPLLTGAGQTHPITRLYEDRAANQKTWNELPPVSVSQTL
                     TNVKPGATVLLEARKVDGSGPSVPLLVQQRYGRGQTLALTASDTWRWRMRMDSKNNAH
                     ETFWRQMLRYVVSNTPRQIEVGTAKDVYSVDDTVNIVADLRDKKFMPVGDANATARVT
                     KPSGATVDVPLKFTTLNSANIYTGEFKADELGQHRIELVGTSKSLGQLNANANVLVSD
                     LNREYYSAAQNTDLLKRIAAETGGKYYTTAETQSLLDDLTYRQTPYSERVTKDLWDMP
                     VNFMLLVGLLSAEWFLRKREGLA"
     gene            4295..5236
                     /locus_tag="VFO72_00345"
     CDS             4295..5236
                     /locus_tag="VFO72_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477759.1"
                     /translation="MPLARQPLQDVRARRPRSQVDPNKFAVIINGAGGEPAYAKQFEQ
                     WTTELQSALSDRFGFDTQKLIVLPNAKATDVKNTFAKLNTQLDATNVLFVFLIGHGSF
                     DGKEAKFNLVGPDLSASEYNAMLSSLPTKRIVLFNMASASGEFVKSLSAKGRIIITAT
                     KSGQETNATRFAGFFIAALNATDADTDQDGHVSVLEAFVYANRLTADFYTRAGRLATE
                     HALLEDNGDGVGHERAEAGEGLLARATYLDSLSVDEAAANAATAKLLKERTRLEAEIE
                     QLIARKGNMAEAEYEATLERLFIQLAKVNRSIKQAGS"
     gene            5237..>6136
                     /locus_tag="VFO72_00350"
     CDS             5237..>6136
                     /locus_tag="VFO72_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9477760.1"
                     /translation="MRTTLLSLIFILAWICVPSSAQDTDLKTLIETGKYTEAETTAKK
                     LLAKTPEAGSVRHQLAEVLAITGRYTEAIAEFEHAANDLQNSNDRPGARLESDLRRAE
                     LLELTGQEDQARSIYESFVKYYTDKDPETAFELTLIARALVHLERFQDANDMFREAIE
                     ADSDYLDAHLSAGELFTKKYNYADAAQFLSDALQLNPNSARAYLDLALNKRIEGGEEV
                     NAALNRALAINPNYVEALSLKAALALEAGQFDVASTELENALKINSRSLEARSLRAAI
                     YYLQDRDFEPEVAATVAIGPRYGE"
BASE COUNT         1436 a         1725 c         1699 g         1276 t
ORIGIN      
        1 tacacgaccg gattggggag gtgagacctt ggcttgttgc cggtgagaaa tatcaggcag
       61 aagacgaaga cctcttcggc ttggtcgttc gcggtcttgc catattagac gagattgagg
      121 ccgcccggaa acttgccgcc agtgaaccat cacgcatgaa aactagtgcg gtcgcgaatc
      181 tctcgctggg aaaagcgctt tccagtcacg agcaatacgt cgaagcggag cgcctgttga
      241 atacagcggc gcaactcgac aagaagtcca ccaatgcgca catcgcgctg gcagttcttt
      301 accggaaaaa gtctcgttgg cttgctgctt tgaacgcggc gaataaggcc gtcagtctgg
      361 atgctgaaga cagtgaaggc tactatcaga aggcttgcgt gctcgcgcgt cttggccgca
      421 ttaaagaagc gatggcggca ctcacgaaat cctttgaact cgatgaagag caaacaatgt
      481 acattgccga ggaagacgat ctgaagccgc tcgcctccct tcctgcgttc aaaaagctcc
      541 ttcccgcacc ggaaaaacaa cagccatagt tgtccacttg tcgtaatttc tctgcgccgt
      601 tcggtcgtca ttgccgcaag tcttcaccaa aactggccgg aatgctgctt gccctgtgct
      661 cctttgaaag tgctcttatc atttcgaagg agaacgtcat gacacacaaa tcactccccc
      721 tgtttctcgt gctcgtattg ttttcgactc acgcgccgct tgcgctggca caacagtccg
      781 tttcaacaaa taactggacc gccgtgcagc aactcaagac caacgagcgg ctggtcgtta
      841 agcaaaagaa cggcaaggaa gtcaaaggac ggatgatcga agccacggac accacactga
      901 caatcgatcg tgacggcaag ccggtggcga tcgcgcgtga tgacgtgcgt cgggtctccg
      961 tgatcgagcg caaagccgaa aagggcaagt acgccctgat cggtgcggga atcggcgcgg
     1021 gcaccggcac ggccattggt tacgcggcat acgaccctca tggagacaat gaagtctaca
     1081 ttctcatggg ctctctaatc ggcgtcggca caggcgccct cggcggcttc ctcttcggac
     1141 tcggcaagcg caaacgcgtc atggtatacg cggtggatta agtctgcgct ggtgaaggac
     1201 ggcgattacg cggacagtgt ctgcacttaa ggtgaagtag attgagtatg gaaatcgcct
     1261 cattactgcg cgccgcagta agttgtcaac aagtagaaac tggtaagggt gtctagcgat
     1321 tcggctcaga cacgcgttaa gttccgtttg gaaacggccg gggaggtttg tgtccttttg
     1381 actgtaccac gtaagcgcta agcgaatatg tttcttggca tccggtgaga ggatgagttg
     1441 acgcattgtt tcttctagag tgaggttcta gcctttaggt tgactcttgc tcaagttctt
     1501 gctgaaagtc ctcccatgtg gttccttcaa aaccaacttc acgataacgg gccatcaact
     1561 ccgtcagctg ctcacggtgc cactctggaa tgtcgacgta ctttgcacct gactgcacgt
     1621 aattcaaaag ctgctcgata taatcgagtt gctcagcgcg tgagagggca tcgaaccctg
     1681 atggcggaac tggtaatggt gaagacatct ttgaactcct tgcacgcagt gtagcacagc
     1741 tctctgcgcc aatggataag tggggttcag gggccggaga gtttgccagt cggggattta
     1801 aatccgcctc acaaacaacc gcccgcctga ggtctgctac taagaacaag tagctcaaag
     1861 ccggtggcag tcttaagcac acgctccgcg cgtgtataat gtccggcacc aaaaaaatgg
     1921 actcgatggt gcggtttctg ttcggacatg aacaggccgt ttttactaac ggccggtttg
     1981 ggtttgacgt gcgtcccggc gccctgctgc tggtcatcat attcttactt gccgccgctt
     2041 tcatctactt catctacctg cgaccacgca taaagctctc aaaacgaacc acggccacgc
     2101 tgatcgcgtt gcgcgccgca ctgctcacga tgatgatcgt actgctgttg cggccagtcg
     2161 tggttgtctc ttcagtgatt ccacgcagca gttatgtcgc cgtcctcgtc gacgattcgt
     2221 tgagcatgaa actgagcgac gtagccggcg gcgcaacacg cctcgacaca gtcaaacagg
     2281 cgctgctcaa cacaactgcc ggacaaactt ctttcctgaa tcgactcgat cagaagttta
     2341 agaccaacct ctacggtttt tcgggtgtac tctcccgcct taaggacggc aacgacctgt
     2401 tcggtgaagg acgcacgagc aatctcgcgg gcgcgctcga agagacaatc aagcggtctt
     2461 ccggaatgcc tctctccgcg attgtactgg ccaccgacgg cgcctcaaac gtacccagcg
     2521 atatcacctc gacgctgcga gaactgcgcg cgcgcgacat tcccgtgttt gcgatcggcg
     2581 tcggcgccac ttcaaaatca ctcgatgcag aactcactcg cgtcaacatg ccgcgccgcg
     2641 tactcgtcgg ctctcgggtg aacatcgagg cttttgtcgg attgagtggt tacgaaacga
     2701 cgaaggtgct gctcggtgtc cgcgaggacg gccgcgcgat taagacggaa cagttcgatc
     2761 tacgcggaaa cgacacgcag gcgatcaatc tcgagatcac tccgaccacc ccgggccttc
     2821 atcgttacac cgtggagatc acaccactcg atggcgagct aacagttgaa aacaacaagc
     2881 aagatgccct ggtcgaagta attgagggtc cgttgcgcct cttgcacgtc gaaggcgagc
     2941 cgcgttggga actcggcaag attcgcgaat cgttggcgct caacgaaaag aacgtagtcc
     3001 tcgtttccct gcaacgcacg ggagagaaca agttctatcg ccagggcatc agcaaccagg
     3061 cagagctggc cacgggattt cccgagaccg aagaagaact tttttcgtat gacggtctgg
     3121 tcgtcggcag tgtcgaagcg ggttttttca gcgccgatca acttcgcagc atcgaggcct
     3181 ttgtcgcacg ccgcggcggt ggtctactcg cgctgggagg gcggttgtcg ttcgatggcg
     3241 gcaagtacaa gggcaccaca atcgacgatc tgctgccggt ttcgttgacg ggtggtccag
     3301 tggacgacgt gaactcgttt gctccggtct acagaccgtt attgactggc gccggacaga
     3361 cgcatccgat cacgcgtctg tacgaagatc gcgccgcgaa ccaaaagacc tggaacgagc
     3421 tgccgccggt ttcggtgtcg caaactttga cgaacgtgaa gcccggcgcg accgtcctgc
     3481 tcgaagctcg caaggtcgat ggatcaggcc catcggtccc gttgctggtg caacaacggt
     3541 acggtcgcgg acagacgctc gccttaactg cgtctgatac gtggcgctgg cgcatgagaa
     3601 tggactcgaa aaacaacgcg cacgaaacct tctggcggca aatgctgcgt tacgttgtga
     3661 gcaacacgcc gcggcagatc gaagtcggca ctgccaagga cgtctattcc gtcgacgaca
     3721 ctgtcaatat cgtcgccgac ttgcgcgaca agaagttcat gccggttggc gacgccaacg
     3781 ccacggcgcg cgtgacaaaa ccgtcgggag caactgttga tgttcccttg aaatttacga
     3841 cactgaactc cgccaacatc tataccggcg agttcaaagc cgacgagctg ggccaacacc
     3901 gaattgagct agtggggacc agcaagagtc ttggtcagtt aaatgcaaat gcgaacgtgc
     3961 tggttagcga cttgaatcgc gaatactaca gcgcggcgca gaacaccgat ctactgaaac
     4021 gcattgcggc ggaaactggc gggaagtatt acacgaccgc cgagacacaa tcgctgcttg
     4081 acgatctcac ttaccggcaa acgccgtatt cagagcgagt gactaaagac ttgtgggaca
     4141 tgcctgtgaa ctttatgttg ctggttgggt tgttgagtgc tgagtggttc ctgcggaaac
     4201 gcgaagggtt agcgtagtta aggtgatact tacgtacata gcactcttga ttatttcgct
     4261 gctcgctcct tgggagcgcg ggcgtctcgc gagcatgcct ctcgccagac agcctttgca
     4321 ggatgtgcgg gcgcgacgcc cgcggtctca ggttgacccg aacaagtttg cggtgatcat
     4381 caacggcgcc ggcggcgaac cagcttatgc gaaacagttt gaacaatgga ccaccgaact
     4441 gcagtccgcg ttgtccgatc gattcgggtt cgacacgcag aagctgatcg ttctacccaa
     4501 cgcgaaggcg accgatgtaa aaaacacgtt tgcgaaactc aacacgcagt tggacgccac
     4561 gaacgtcctg ttcgtgtttc tcatcggtca cggttcattc gacggcaagg aagcgaagtt
     4621 caacctcgtc ggtccggatc tttcagcatc cgagtacaac gccatgttgt cgtcgctgcc
     4681 cacgaagcga atcgtgcttt tcaacatggc gagcgcgagt ggcgagttcg ttaagagcct
     4741 gtccgccaaa ggacgcatta tcatcacggc caccaaaagc ggacaagaaa ctaacgcgac
     4801 acggtttgcc ggtttcttca tcgcagcact caacgcgacc gacgccgaca ccgatcagga
     4861 cggtcacgtt tcagtgctgg aggctttcgt ttacgccaac cgcttgaccg ccgactttta
     4921 cacgcgcgcc ggcaggctcg cgacggaaca cgcactgctc gaagataatg gcgatggcgt
     4981 gggccacgaa cgagcggaag ccggtgaagg gctgctggcg cgtgctacgt atctcgattc
     5041 gttgagcgtg gacgaagcgg cagcgaatgc cgctaccgcc aaactgctga aagaacgcac
     5101 gcgtcttgaa gccgagatcg aacaactgat cgcgcgcaag ggaaacatgg ccgaggccga
     5161 atacgaagcg acactggaga gactttttat tcaactggca aaagtgaatc gttcgattaa
     5221 acaagcagga tcgtaaatgc gcacaacgtt attgagtctg atttttattt tggcttggat
     5281 ctgtgttccg tcgtctgccc aggacacaga tctcaaaacc ctgatcgaaa ccggtaaata
     5341 caccgaggcc gaaacgacag ccaaaaagct cctcgcaaag acccccgaag ccggcagcgt
     5401 gcgccatcaa ctcgctgaag tactggcgat taccggccgc tacaccgaag ccatcgcgga
     5461 gtttgaacac gctgccaacg acctccaaaa ctccaacgac agaccgggcg cacgtctcga
     5521 gagcgatctg cgccgcgccg aactgctcga gctaaccggc caggaagatc aggcgcggtc
     5581 tatctatgaa tcgttcgtca agtactacac cgacaaggac cccgaaaccg catttgagtt
     5641 gacactcatc gcgcgcgcgc tggtccacct cgaacgattc caggatgcga acgacatgtt
     5701 tcgcgaggca atcgaggccg attctgatta cctcgatgcg cacctctccg ccggtgagtt
     5761 gtttactaaa aagtacaact atgccgacgc agcccagttt ctctccgatg ctttacaact
     5821 gaatccaaac agcgcccgcg cttatctgga tctcgcgctc aacaaacgaa tcgaaggcgg
     5881 cgaggaggta aacgcggcgt tgaatcgcgc gctcgcaatc aatcccaatt acgtcgaagc
     5941 actgtcgctc aaagcagcac tcgcgctcga agccggacaa ttcgacgtcg cctcgaccga
     6001 actcgaaaat gcgttgaaga tcaattcgcg ttcgctcgaa gcccgctcgc tgcgtgctgc
     6061 gatctattac cttcaggacc gcgattttga accggaagtc gcggccacgg tggcgattgg
     6121 tccgcgatac ggcgag
//
LOCUS       DASTEY010000021         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1467083, whole genome shotgun sequence.
ACCESSION   DASTEY010000021 DASTEY010000000
VERSION     DASTEY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467083"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1716)
                     /locus_tag="VFO72_00355"
     CDS             complement(25..1716)
                     /locus_tag="VFO72_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9477761.1"
                     /translation="MTSLSRRRFVLSSAMVSVAVLVNPRNLLGQSAAVQPLFARLDDY
                     IARHMRETGMPGLTLALANRDGLIRASTYGFADTKTRSRVVPETMFEIGSISKSFVGL
                     LLLQLRDEGKLDLNKPVVEYLPWLKINSKFEPITTHHLLSHTAGLPGAPLLLDALLHE
                     LWTAYEPGKRFLYSNTGYNILGFLIEAIDKRRFADSLRARILAPLGMTASSPIISNEL
                     RAQIAVGYEPLYEGRPFSLGGPLAEAQWIEVDIAAGSIASTPADMAKYIRMLLNRGAL
                     PKGRLLSEEGFTLFTKAAINSPYRGEPASYGYGLWVSDIGAHTRLRHTGGMVAFSSSL
                     DVDLTDGVGAFASVNANLRGYRPVAVTRYAIELFNASLAGNSLPEAPAPLPAPDEVKN
                     AADYVGVYTSPDGRKLEFSAEGDKLILTYNGRKIALERVGGDRFIVKHPAFDLFVLGF
                     VRENQKVTQVFHGPDWYAGARYTGARKFEARKEWEGLAGHYFHDSPWYGDTRVVLRKG
                     QLFLEGVQPLVPRGDGKFGIGDPEAPDWISFESIVDGRAMQLNYSGIKFRRMFTP"
     gene            complement(1722..4145)
                     /locus_tag="VFO72_00360"
     CDS             complement(1722..4145)
                     /locus_tag="VFO72_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9477762.1"
                     /translation="MIGRILLQYRIVEKIGEGGQGTVYRAVDTTLDRPAVVKVLPPEL
                     TDRASNLVRFEREAKLASSLDHPNICTIFGLHKVDGIHFIAMQYIEGRNVRELVNGRP
                     LGLRQALSITIQVTNALAAAHAQGIVHRDIKARNVMVTRNGTVKVLDFGLAKLIEPLH
                     AGSSDPQLTEVGVPYGTSTYAAPEQAQGLPVDHRGDIFSTGVLLYEMLAGAWPFRGRT
                     ALDVRYAVVYQQPRPIAQARGEDSPLIQRIQAILDNALAKQPDDRYQRIEDLRADLQE
                     VLRELEGDASVGNTLTDATLSIAPPPRRKQQSLWTRPWLMMACVAAVATVVVALLVTG
                     FRDSAPAESSAAINSLAVLPFSNSDPSAEYLSDGITESLIENLSKLQDLKVKSRSTVF
                     HYKGRNADPKEIGRELGVHALLYGDVVQRGDELAVSVELIDVRDDSHIWGERYGRKVS
                     EVVTLPQQISRDVSLKLRARADGIDYAHITRNYSPDSEAYRLYLLGRYNWNKRTVEGL
                     QEGIIYFRQAIMRDQDYGLAYAGLADCYLLLNVYNVASADASYPKAEAAANRALSINP
                     NLAEAHTSLGFVSYRYHLKWDEAEQHFKKAISLNPSYATAHQWYASYLAASGRVNEAV
                     AEAKLAHDLEPFSLTIHSDYIRNLYYAGQLDQAHSEALKLREMDRDFARAYYELGLVL
                     EEQGKFDEAITAFRTALKSSPDNITALTALGHALALAGKRNEAERVIAKLQELSKQQY
                     VSPFQTAVIYAGLDNRKMALDWLEKSRDERFNWLPFVQIDPVFKNLRTEARFIELAKN
                     LGLATEKNE"
     gene            4208..5071
                     /locus_tag="VFO72_00365"
     CDS             4208..5071
                     /locus_tag="VFO72_00365"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HET9477763.1"
                     /translation="MILRISLLTAILASLTFSPAATDKHPDPSGAADSLGGTSSFYSF
                     RRDLRRCAAPRCGGYFVKLVNRLRTRCADYSYQSECYVASIDWGGQAEPDSDRALLRG
                     SISPQGRFAILRVSEVWQGASANQPTGRFFRVRDRGIRCIAAPCATHHEARLNSTNSR
                     NIAGVDLSGAGAPDNQVSEANEAMTGRDGIIVSGFHSRVTGPAGRMQMLRATQFYLRA
                     GNNVSQKPCMKTGCSGQVCSDEEVITTCEYSPEYECYKKAICERQRNGQCGFTQTPEL
                     AECLRRARRQP"
     gene            5319..>6215
                     /locus_tag="VFO72_00370"
     CDS             5319..>6215
                     /locus_tag="VFO72_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477764.1"
                     /translation="MRVLAGVLIALFMVAPRWSLQTSGVTARLRGVSAVSERVAWASG
                     SGATVLRTADGGATWRKLNVTSEALDFRDIDAIDVRTAYVLSIGNGPASRIYKTTDAG
                     ATWTMQFKNDNPKVFLDAMSFWDENNGIVFGDSVDGQFYILMTDNGGRTWSRVSTTAL
                     PPALQNEGAFAASGTNIAVFGKTHAWIGTGAGAKARVLRTSDRGRSWQIADTPLAAGA
                     SAGIFSIAFRDAKNGVVVGGDYRKEQEAVDNLAITDDGGATWKLAKGLSGYRSVVAYV
                     PVTKTPALIALGPTGGDYSLDGG"
BASE COUNT         1325 a         1744 c         1801 g         1345 t
ORIGIN      
        1 cactcttgag gcatcaatga ctaactaagg tgtgaacatg cggcggaatt tgatgccgga
       61 gtagttcaat tgcatcgcgc ggccgtcgac gattgattcg aaactgatcc agtctggcgc
      121 ttcggggtcg ccgatcccga atttcccatc gccacgcggc acgagcggct gaacgccctc
      181 gaggaacagt tgccccttgc gcagcaccac gcgcgtgtcg ccataccacg gactgtcgtg
      241 gaagtagtga cccgccagcc cttcccattc cttgcgcgct tcaaatttcc gagcaccggt
      301 gtaacgcgcg cccgcgtacc aatccggtcc atgaaacact tgcgtcactt tctgattctc
      361 tcgcacgaac ccaagcacaa acagatcgaa cgccggatgc ttcacaatga agcgatcgcc
      421 accgacgcgc tccagcgcga tcttgcgtcc gttgtatgtg agtatgagtt tgtccccttc
      481 ggccgagaac tcgagtttcc tgccgtcagg tgacgtgtaa acgccgacat aatcagccgc
      541 gttctttact tcatcaggcg cgggcaaagg cgcgggcgct tcgggcaatg aattgcccgc
      601 gagtgaagcg ttgaagagtt cgatcgcata tctcgtaaca gccactggtc gatagccacg
      661 aagatttgcg ttaacggaag caaacgcacc cactccgtct gtgaggtcca cgtcgagcga
      721 tgaactgaac gcaaccatgc cgccggtgtg acgcagacgc gtgtgggcgc cgatatcact
      781 cacccacagg ccgtaaccat acgacgcggg ctcaccacga tatggcgagt tgatcgccgc
      841 cttcgtaaac agcgtgaatc cctcttccga aagcaggcgg cctttcggca acgcaccgcg
      901 attgagcagc atgcggatgt acttcgccat gtctgccggt gttgaagcga tactgcccgc
      961 agcgatgtcc acttcaatcc actgcgcttc agccaacgga ccacctaacg aaaacggccg
     1021 tccttcgtac agcggttcgt agccgacggc aatctgcgca cgcagctcgt tgctgatgat
     1081 cggcgagctc gcggtcatgc ccaacggcgc gagtattcgc gcgcgcagcg aatctgcgaa
     1141 ccgtcgtttg tcgatggctt cgatcagaaa gccgagaatg ttgtagcccg tgtttgagta
     1201 aagaaaacgc ttgcccggct cgtaagcagt ccacagttcg tgcagcaaag catcgagcag
     1261 caacggcgca ccgggcagtc cggccgtgtg gctcagcaag tgatgtgtcg ttatgggctc
     1321 aaactttgag ttgatcttca accacggcag atactcgacg accggtttgt tgagatcgag
     1381 cttgccttcg tcgcgcaact gcaacaagag gagcccgaca aacgacttcg atatcgatcc
     1441 aatctcaaac atcgtctcgg gcactactcg cgaccttgtc ttcgtatccg cgaagccgta
     1501 cgtcgaggct cgtattaaac cgtcacgatt cgccagcgcg agcgttaatc ccggcatgcc
     1561 ggtctcgcgc atgtgccgcg cgatatagtc gtcgagcctg gcaaacagtg gctgcaccgc
     1621 cgccgattgg cccaagagat ttcgtggatt gactaaaacc gcgacgctga ccatcgcact
     1681 gctcaggaca aagcgccgcc gcgaaaggct tgtcatctgt gtcattcgtt tttttccgtg
     1741 gctaaaccga gatttttcgc cagctcaata aacctcgcct cagttcgcaa gttcttaaag
     1801 accggatcga tttgcacgaa cggcaaccag ttgaaccgct cgtcacgaga tttctcgagc
     1861 cagtcgaggg ccatttttcg attgtcgaga ccggcgtaga tcaccgcggt ctgaaatggg
     1921 gagacgtatt gttgtttcga aagttcctgc agctttgcga tcactcgttc ggcttcgttc
     1981 ctttttcccg ccagggccag cgcgtgaccg agggcagtca gcgcggtgat gttatcaggc
     2041 gaacttttca gcgcggttcg aaacgcagtg attgcctcat caaacttgcc ctgctcttcg
     2101 agcaccaatc cgagttcgta atacgcgcgc gcaaaatcgc gatccatctc gcgcagcttc
     2161 aacgcctcgc tgtgcgcctg atccaactga cccgcgtagt aaagattgcg aatgtaatcg
     2221 ctgtggatcg tcaacgaaaa cggttcgaga tcgtgagcca gcttagcttc ggcgactgct
     2281 tcattaacgc gtccggacgc cgcgagataa ctcgcgtacc actggtgcgc ggtagcataa
     2341 cttggattga gtgaaatcgc tttcttgaaa tgttgctcgg cctcgtccca cttcaaatga
     2401 taacgataag acacaaaacc cagcgacgtg tgtgcttcgg cgagattcgg atttatcgac
     2461 aatgctcggt tcgccgccgc ttcggctttc ggatacgagg catctgccga ggcaacgtta
     2521 taaacgttga gcaagaggta acagtcggcg agtccggcat atgcgaggcc gtaatcctgg
     2581 tcacgcatga tcgcctggcg gaagtatatg atcccttcct gcaacccctc gacagttcgc
     2641 ttgttccagt tgtagcggcc cagaagatag agacgatacg cttcggagtc cggtgaatag
     2701 tttcgcgtga tgtgcgcgta gtcgatgccg tcggcacgcg cccgcaactt gagcgaaacg
     2761 tcgcgcgaaa tctgttgcgg gagtgtcacc acttccgaaa ccttgcgacc gtagcgttcg
     2821 ccccagatgt gactatcgtc gcgcacgtcg atcaattcca cgctgacggc gagctcgtcg
     2881 ccacgctgca cgacgtctcc gtaaagcagc gcgtgtacgc cgagttcgcg cccaatctct
     2941 ttcgggtccg cgttccgtcc tttgtagtga aacaccgtgc tacgcgactt gaccttcaga
     3001 tcctgcaact tcgaaaggtt ctcaattaaa ctttccgtga tgccgtccga gaggtattcg
     3061 gcactgggat cggagttgga aaatggcagt acggcaagtg aattgatcgc cgcgctggac
     3121 tcagcgggcg ccgaatcacg aaaaccagtt accaacaacg ccacaactac cgttgcgact
     3181 gcggcgacgc acgccatcat taaccagggc ctggtccata agctttgctg cttgcgtcga
     3241 ggaggcggcg cgatcgatag cgtcgcgtca gtcagggtat tgccgacgct cgcatctcct
     3301 tcgagttcgc gcagcacctc ttggagatcg gcacgcaggt cttcaattcg ctggtaacga
     3361 tcgtccggtt gcttggccag cgcgttgtcg aggatcgcct ggatccgttg gatcagcggc
     3421 gagtcttcac cgcgcgcctg cgcaataggc cggggctgct ggtagacgac ggcgtaacgc
     3481 acgtccaaag ccgtcctacc acgaaaaggc caggcgccgg ctaacatctc gtacagcaga
     3541 acaccggtcg aaaaaatgtc gccgcgatgg tccaccggta agccttgcgc ctgctcgggc
     3601 gcagcgtaag tcgaagtgcc gtaaggtaca ccgacttccg tgagttgcgg gtcgcttgaa
     3661 cctgcatgta gaggttcgat cagtttcgca agtccgaagt cgagcacttt aacggtgccg
     3721 ttgcgcgtga ccatcacgtt gcgcgctttg atgtcgcgat gcacgatgcc ttgtgcgtgc
     3781 gcggcggcaa gcgcgtttgt cacctgaatc gtgatcgaga gtgcctgacg cagaccgagt
     3841 ggacggccgt tcaccaactc gcgcacgttg cggccctcga tgtactgcat cgcgatgaag
     3901 tgaatgccgt cgactttgtg gaggccaaag atcgtacaga tgtttgggtg gtccagcgag
     3961 gacgcgagtt tggcctcgcg ttcgaaacga accagatttg aggcacggtc cgtgagttcc
     4021 ggcggcagga ccttgactac tgcgggccga tcgagtgtag tgtccaccgc acgataaaca
     4081 gtaccctgtc ctccttcacc gatcttttcg acgatgcgat actggaggag gattcgcccg
     4141 atcataggtg tgcgtgaata gcgaagcgag cgcattatat ccaacttaac tcctggaggg
     4201 cagaaagatg atcctgcgca tttccctgct gactgcgatc cttgcatcgc tgaccttctc
     4261 accggcagct accgacaaac atccggaccc ctcaggcgct gccgacagcc tcggcggcac
     4321 ttctagtttc tacagttttc gccgtgattt acggcgttgt gcggcgccgc gctgtggtgg
     4381 ttatttcgtt aaattagtga accggttgcg cacgcgttgt gctgattaca gttaccagag
     4441 cgagtgctac gtcgcgagta tcgactgggg tggacaagcc gaaccggaca gcgatcgggc
     4501 gctgttgcgc ggatcgatta gcccgcaagg gagatttgcg atcttacgcg tgtcggaggt
     4561 ctggcagggt gcgagcgcca accaaccaac gggtaggttc tttcgtgttc gcgatcgcgg
     4621 catacgctgc atcgcggcgc cgtgcgcgac gcatcacgaa gcgagactga attcaacaaa
     4681 cagccgcaac atcgcgggcg ttgatctatc aggcgcgggc gcgcccgaca accaggtgag
     4741 cgaagctaac gaagcgatga ctgggcgaga cggcataatc gtcagcggtt ttcattcacg
     4801 cgtaactgga cctgcagggc gaatgcagat gctcagagca acgcagtttt acttacgtgc
     4861 aggtaacaac gtgtcgcaga aaccgtgtat gaagacgggc tgctcgggcc aggtttgttc
     4921 ggatgaggaa gtgatcacga cgtgcgagta cagccctgag tatgaatgct acaaaaaggc
     4981 gatctgcgaa cgacaaagaa acgggcagtg cggctttacg cagacacccg agctcgctga
     5041 gtgtttgcga cgagcgagac gacaacctta gcgatgggta gtgagacgga acaccgtgat
     5101 gactacgccg aaggcgttag caaattccag cccagggttg cttcaaccct gggttcagga
     5161 atgaaagaat taagaaacgc tgaaagcgtt ggcggttgtg cggtgctgac caacgctttc
     5221 agcgttcggc tgtatttccg ttccgtatcc cagggttgaa gcaaccctgg gctggacttg
     5281 accaacgctt tcagcgttaa ttcaaacact acttagcgat gagagtactg gcaggcgtct
     5341 tgattgcatt gttcatggta gcgccgcgat ggagtttgca aacgagtggc gttactgcgc
     5401 gtttgcgcgg cgtgagtgcg gtgagtgaac gtgtcgcttg ggccagtggt tccggcgcta
     5461 ccgtgctgcg taccgctgat ggtggcgcga cgtggcggaa gttgaacgtt acgagcgaag
     5521 cgctcgactt tcgcgacatc gatgcgattg atgtgcgaac tgcgtacgtt ttgagtatcg
     5581 gtaatggtcc agcatcgcgc atttacaaaa ctaccgacgc gggcgcgacg tggaccatgc
     5641 agttcaagaa cgacaatcca aaagtgtttc ttgatgcgat gtcgttctgg gacgaaaata
     5701 acggcatcgt ttttggtgat tcagttgacg gtcagtttta cattctgatg actgacaacg
     5761 gcgggcgcac ctggtcgcga gtgtccacga ctgcgttgcc gccggcattg caaaacgaag
     5821 gcgcgttcgc ggcaagcggc acaaacatcg ccgtttttgg caaaacacac gcttggatcg
     5881 gcactggcgc cggtgctaaa gcgcgcgtgt tgcgtacgtc cgatcgtgga cgttcgtggc
     5941 agatcgctga tactccactc gctgccggag cgtcggcagg gatcttttcc atcgcttttc
     6001 gcgacgcgaa gaatggcgtg gtggtgggcg gcgactaccg caaagagcag gaagcggtcg
     6061 ataacctcgc cataactgac gacggcggcg ccacctggaa gctcgcgaaa ggtctttcag
     6121 gctatcgttc ggttgttgct tatgtcccgg taacgaagac gcccgcgctc attgcgcttg
     6181 gtccaacagg tggagactat tcactcgatg gcggt
//
LOCUS       DASTEY010000022         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1137515, whole genome shotgun sequence.
ACCESSION   DASTEY010000022 DASTEY010000000
VERSION     DASTEY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137515"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..2420)
                     /locus_tag="VFO72_00375"
     CDS             complement(270..2420)
                     /locus_tag="VFO72_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9477765.1"
                     /translation="MTDHSEMDIAEVERYELAEDPLYQFSFDRRQFLKAFSAGIALVV
                     PLSHLLAQEPESRGESGRRFNRELPQEIGAWIHVDQDGSVVVYTGKVEFGQNIRTSLA
                     QAVAEELHVPVSTIRLVMGDTDLTPFDMGTFGSRTTPTMAPQLRKAAAAARETLIALA
                     AQQWQVAPDTVRIVDARFVNHDGTKSMTLADVAKGQKLVKTISPDVKTTPVDRWTVGG
                     KSVPKVDGRDFVTGRHKYTSDMRREGMLVGAVVRPPRVDATLEGRTSGTTQPPGVQVV
                     EDGNFLGVAALDQQTATRAAKSTAARWKTPAEQPPDNAALFDYFRKNARDGRGRPVGS
                     VADGFAAADKTLEQTYTVAYIAHAPLEPRAAVAEWVDNKLTVWTGTQRPFGVRGELSE
                     ALHIPANRIRVIVPDTGSGYGGKHTGECAIEAARLAIATKKPVKVVWTREEEFNWAYF
                     RPAGVIDVKTGVKNDGKITAWEFHNYNSGSAGIQLKYDIQNQNIRFHDTKSPLRQGSY
                     RGLAATANHFARETHFDELAAMLKLDPLEFRLKNIKDERLRAVLEAAATRFGWGKSAT
                     GAGRGAGISCGFEKGGYVATCAEVSVESRTGKVKLERVVESFECGAIVNPMHLENQIE
                     GAVVQAIGGALFEAIEFANGTILNPRFARYRLPRFSDVPPIEIVLVNRKDLPSAGAGE
                     TPIVGLAPAVGNAVFAATGKRLRSLPLAPKGLQT"
     gene            complement(2417..2893)
                     /locus_tag="VFO72_00380"
     CDS             complement(2417..2893)
                     /locus_tag="VFO72_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9477766.1"
                     /translation="MSKVTALNVNGRKLSVNVDPQTSLLSVLRNDLDLSGTKYGCGEA
                     QCGACTVLIDGQQTRSCVTQVGKVAGKQITTIEGLEKDGQLHPVQEAFLKADAMQCGY
                     CTPGMIMSGVALLNANSKPTREQIVRHMDRNICRCGVYLRIIAVIQMASGQKGDAE"
     gene            complement(3533..4489)
                     /gene="pip"
                     /locus_tag="VFO72_00385"
     CDS             complement(3533..4489)
                     /gene="pip"
                     /locus_tag="VFO72_00385"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET9477767.1"
                     /translation="METLRTLYDEIEPYDSGHIKVSAVHELYYEQCGTPNGKPVVFLH
                     GGPGAGLVPDYRRFFDPNAYRIILFDQRGAGLSRPHASLEDNTTWHLVQDTDMIREHF
                     GIDQWLVFGGSWGSTLALAYAETHPERVRGLVLRGIFLCRPKEIRWFYEDSQGASAIF
                     PDGWEQYVQIIPESERGDMIRAYYHRLTSDDESVRLEAARAWSIWEGSALKLLPDQKL
                     IDEFSEPEKAIALARIECHYFVNNCFFETDDYLLEHVERIRQIPGVIVHGRYDIVCPF
                     MSAWHLHRAWPEAELKVIPNAGHSVFEPGITDALIRATDSFR"
BASE COUNT          991 a         1396 c         1272 g         1043 t
ORIGIN      
        1 gtcttttccg cctgttcttc ttcctggtcc tgggcatgaa ctgacgcgaa accaatggca
       61 accgccacaa gagctaaaca caggtaccgc aatttgactt gcataacatc ctccgccttc
      121 aaggcgactc aacgtttggg ctcgaagtcc catttaaaac gcggagatcc tacgagaggg
      181 aggtgtttgc gctaatgtta cagaccacag atccgcaccg taccgaccac agtcagcatg
      241 tgaggaaggc cggatctcgg tgatttaggc taggtttgca gacccttcgg cgctaacggt
      301 agggagcgga gccgcttgcc ggtggcggca aagacggcgt tgccgactgc cggagcgagg
      361 cccacgatcg gtgtttcgcc tgcaccggca gatggaagat ccttgcgatt cacgagcacg
      421 atctcgatcg gcggtacgtc gctgaaccgc ggcaatcgat aacgcgcaaa gcgcgggttg
      481 agaatagtgc cgttggcaaa ctcaatggct tcgaaaagcg cgccgccaat ggcctgcacg
      541 actgctcctt cgatctggtt ttccaagtgc atcggattga cgatggcgcc acactcgaac
      601 gactcaacaa cccgttcgag tttgactttg ccggtgcggc tctcgaccga aacttcagca
      661 cacgtcgcta cataaccgcc tttctcgaaa ccgcacgaga tacctgcgcc tctgccggcg
      721 cccgtcgctg atttgcccca gccgaaacgt gtcgccgcag cttccagcac cgcgcgcaga
      781 cgttcgtctt tgatgttctt caaacgaaac tcgagcggat ccagcttcaa catcgccgcg
      841 agttcatcga aatgcgtctc gcgcgcgaag tgattcgcgg tagcagccag tccacgataa
      901 gaaccctgcc gcagcggcga cttggtatcg tgaaaccgta tgttctgatt ttgaatgtcg
      961 tacttgagct gtattccggc cgatccggaa ttgtagttgt gaaactccca cgcagtgatc
     1021 ttgccatcgt tcttgacgcc ggtctttacg tcgatcacgc ctgccggccg gaagtaggcc
     1081 cagttaaact cttcttcgcg agtccaaacg accttcaccg gtttcttcgt cgcgattgcg
     1141 aggcgcgccg cttcgatcgc gcactcgcct gtgtgcttgc cgccgtagcc ggaaccagtg
     1201 tcaggcacga tcacgcgaat tcgattcgca gggatgtgaa gcgcttcgga gagttctcca
     1261 cgcacgccga acggccgttg cgtgccggtc caaacagtta acttgttatc gacccactcc
     1321 gcgacagcgg cgcgcggctc gagcggcgca tgcgcaatgt aagcgaccgt gtaggtttgt
     1381 tcgagcgttt tatctgcggc tgcgaatcca tcggcaactg aaccaaccgg acgcccacga
     1441 ccgtccctcg cgttcttccg gaagtaatca aacaatgcgg cgttgtccgg cggctgctcc
     1501 gccggcgttt tccaacgcgc cgcggtcgac tttgccgcac gtgtcgcggt ctgctggtcc
     1561 aacgcggcca caccaagaaa atttccgtcc tcgacaactt gcacgccggg cggctgcgta
     1621 gtacccgaag tgcggccttc aagcgttgca tcaacacgcg gaggccgaac gacggcgcca
     1681 accaacatgc cttcgcgccg catgtcagac gtgtacttgt gccgtcccgt aacgaaatcg
     1741 cggccgtcaa ctttcggaac tgattttccg ccgaccgtcc aacggtcgac tggagtcgtc
     1801 tttacgtcag gcgaaatcgt tttgacaagc ttctgaccct tcgcgacgtc cgccagcgtc
     1861 atcgacttag tcccatcgtg attcacaaag cgagcatcaa caatgcgaac tgtatcgggc
     1921 gcaacctgcc attgctgcgc agccaacgcg atcagagttt cccgcgcggc cgccgctgct
     1981 ttgcgcaact gcggcgccat cgtgggtgtg gtgcgactgc caaacgttcc catgtcgaat
     2041 ggcgttagat cagtgtcgcc cattacgagt cggatcgttg aaacaggtac gtgcaattcc
     2101 tcagcgacgg cctgggccag tgacgtgcga atgttctgtc caaactcgac ctttcccgta
     2161 tagaccacca ccgatccgtc ttgatccaca tgtatccagg cgccaatctc ctgcggcaac
     2221 tcacgattaa atctccgccc ggattcgcct cgcgactcag gctcctgcgc gagtagatga
     2281 ctcagcggca cgacgagcgc gatgccggcg ctaaacgctt tcagaaactg gcgtcgatcg
     2341 aaactgaatt gataaagcgg atcctcagcg agttcgtagc gttcaacttc agcaatgtcc
     2401 atttcactgt ggtcggtcat tcggcgtctc ctttctgtcc cgacgccatt tgaatcacgg
     2461 cgataatgcg tagatagaca ccgcaacggc agatgtttcg atccatgtgc cgcacgatct
     2521 gttcgcgcgt gggtttggag ttggcgttga ggagagccac gcctgacatg atcatgcccg
     2581 gcgtgcagta gccacactgc atcgcgtccg cttttagaaa cgcctcctgg accggatgta
     2641 actggccatc tttctccaag ccctcgattg tcgtgatctg tttgccggcg actttaccaa
     2701 cctgggtgac acacgagcgc gtttgttgtc catcgatcag caccgtacag gcgccgcatt
     2761 gcgcttcacc gcaaccatac ttggtgcctg agaggtcgag atcgttacgc agcacgctca
     2821 acaaactcgt ctgtgggtcg acgttaacgc tcaacttcct gccgttgacg tttagcgcgg
     2881 tgactttcga cataaatgcc agcctcccga attactcgtt tggctggcat tgtaaagtgc
     2941 ggaagaactt atgtcatggg gtaccgacac agtgaaccag tttgacctgg gaccgcaggc
     3001 gtctcgcctg ccaaactcac ggtagagcgc tgctttgcct gaaaactcct ctctcattct
     3061 caccgggctt ttagcccggt ggaagagcag tgaagacgga ggaaaccgtt tcaacggttt
     3121 ttgagggatt cgcgagcgaa accgttaaaa acggtttctc ccaactgcgt gccctttcac
     3181 cgggctgaag cccggtgaga atgagactgc cccaaactgg ctttgcccga aaagtgattt
     3241 ttacttgatg cttctgctga gaagtgctcc ggaggagcaa gatgcttata gccttacgtt
     3301 tttattaact agtgcgctcc agcggagcgc aacgtgctct ggccgtttgg tttacatacc
     3361 gctcctgacg gagcggcgta attcaggaac cgaagagcta taaatatttt gctcctccgg
     3421 agcaagacgc ttaggtacaa ataaaatggc ttttcaagca aagcccccaa actcagatcg
     3481 cgaaaaaccg aagcgataag tatcaggggt tcttatgttt ttcaacgctc gattagcgaa
     3541 agctgtccgt ggcgcggatt aaggcgtcgg tgatgccagg ttcgaagacg gagtggccgg
     3601 cgttaggaat gaccttcagc tccgcttcgg gccaggcccg atgcaggtgc caggcgctca
     3661 tgaacggaca gactatgtcg taacggccgt ggacgattac gcccggaatc tggcgaatcc
     3721 gctcgacgtg ttcaagcagg taatcgtccg tttcgaaaaa acagttgttg acgaagtagt
     3781 gacactcgat acgagccaac gcaatcgcct tttccggctc gctgaactcg tcgattagct
     3841 tctgatccgg caggagcttt agcgcgctcc cttcccagat agaccacgcc cgcgctgctt
     3901 cgagccgcac cgactcgtcg tcgctcgtca accggtgata gtaggcgcga atcatgtcgc
     3961 cgcgctccga ctcgggaatg atctgcacat attgttccca gccatccgga aagatcgcgc
     4021 tcgcgccctg actgtcttcg tagaaccagc gaatctcttt tggacggcac agaaagatgc
     4081 cgcgcagcac cagcccgcga actcgttccg gatgtgtttc ggcataagcc aacgcgagtg
     4141 tgctgcccca cgaaccgcca aatacgagcc actgatcgat gccaaaatgt tcgcggatca
     4201 tatccgtgtc ctggaccagg tgccaggttg tgttgtcttc aagtgaagcg tgcggacggc
     4261 tcagacctgc accgcgctgg tcgaagagaa tgatgcggta agcgttcgga tcgaaaaagc
     4321 ggcggtagtc cggcacgagg ccggcgcccg gtccaccatg cagaaacaca accggttttc
     4381 cgtttggcgt gccacattgc tcgtaataaa gctcgtgaac agcggacact ttgatgtgcc
     4441 cgctgtcgta aggttcgatt tcgtcgtaaa gggttctcag tgtttccatg gtaagcggcg
     4501 attatcgtgt cacaacacgc cgaactgcaa acgccgaaga cccgttatca cctcctcgcg
     4561 accgccgtgt tttgaaatgc ggctatggtc caaccctgct tctccttcgt cattacgatc
     4621 gtaagcatcg cgtctgccgt caactctttg gttgggcctt cccggtgagc actgacgtgc
     4681 accaccgcca catccggtcg ca
//
LOCUS       DASTEY010000023         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_643070, whole genome shotgun sequence.
ACCESSION   DASTEY010000023 DASTEY010000000
VERSION     DASTEY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643070"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1018)
                     /locus_tag="VFO72_00390"
     CDS             complement(170..1018)
                     /locus_tag="VFO72_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET9477768.1"
                     /translation="MRWRGERQSTNIEDRRGLTGSKIAVGGGLGTVIVIIIALLFGAD
                     PQQLLEQLPSDRSAEVQTSRPRNAEEEELKQFVSVVLGKSEEVWHEIFRRNGRQYREP
                     TLVLFTDQVQSACGMAGAAVGPFYCPGDEKVYIDLAFYEALRRRFNAPGDFAQAYVIA
                     HEVGHHVQNLLGISDRVHAMQQRVTEAEANQLSVRLELQADFFAGMFARYVQNQGLLE
                     AGDVEEALQAASAVGDDRIQRRTTGRVVPDSFTHGTSEQRLRWFRKGYDTGDMRQGDT
                     FGTRDL"
     gene            complement(1309..2346)
                     /locus_tag="VFO72_00395"
     CDS             complement(1309..2346)
                     /locus_tag="VFO72_00395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9477769.1"
                     /translation="MRDQIDQARWFAVLLATALALYLCWLMLRPFIGVLAWAVVLVIV
                     FYPVHLRLRDRIKNPSLSSLISCLLVVLLAVLPLTLLTIAVADELGKVLPNLPSQISS
                     LMDPQTSVIGRVAGWIQGRFGVDTLRSQEFMVEQLKRSSEFLLSMSLNLAGNIATGIV
                     KAFFVIFTMYYLFRDGDRIVSKLPAALPLEKKESEAIIARTREVVSASVYGIVVIAAL
                     QGLLGGIAFWVLGIPSPLLWAVLMTFVCMIPVLGSFLVWVPLAIYLIMNGHWTKGILL
                     VVWGALVISTVDNFLRPKWMKNQTRLHELLVFFSVLGGISIFGLLGIILGPVILAITL
                     GLLHTFRAHPS"
     gene            2550..3959
                     /locus_tag="VFO72_00400"
     CDS             2550..3959
                     /locus_tag="VFO72_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HET9477770.1"
                     /translation="MLLTISTTYQPATDLGYLLHKNPARLHTFPLSFGQAHVFYPEAT
                     ADRCTAALLLDVDPVGLVRNRRGPAGEGGTLDQYVNDRPYVASSFLSVAISRVFGSAL
                     GGRSKNRPELAETKIPLAAKISVLPCRGGEDFLRRLFEPLGYQVIAQRHPLDETFPEW
                     GVSPYFTVQLYGVVRLQDLLAHIYVLVPVLDNEKHYWVGDDEVEKLLRRGSGWLSAHP
                     EREEITRRYLKYRRDLAHEALARLTEEAPDVDAAEAIHDSEEEAVERKLNLNEQRLNS
                     VAAALKSAGAKRVLDLGCGEGKLLRVLFADRSFDEIVGVDVSHRALQFAHERLRLERM
                     PERQRERLKLVHGSLMYRDRRLSGFDAAAVVEVIEHLDQPRLAAFERVLFEFAKPKTI
                     VLTTPNIEYNARFENLPAGKFRHKDHRFEWTRAEFHHWARAIADRFGYAVRFLPVGPE
                     DSALGAPTQMAVFKREQAA"
     gene            3956..>4510
                     /locus_tag="VFO72_00405"
     CDS             3956..>4510
                     /locus_tag="VFO72_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9477771.1"
                     /translation="MKTITIPELALVVLIGPSGCGKSTFALSHFKPTEVLSSDFCRAL
                     VSDDENNQAATKDAFEVLHFIAAKRLAAGKFSVVDATNVQPEARKPLVALARAYHVIP
                     VAMVLNLPDRVCQDRNRLRSDRQFGAHVIRQQSQQLRRSIRNLKREGFRHIYEFSSQA
                     EVDEVVIERQPLWNNRKHEHGPFDI"
BASE COUNT          997 a         1251 c         1300 g          962 t
ORIGIN      
        1 aaaacatcca atcagtcccc agggttgtcg caagctccaa cctctgggct ggaattcgat
       61 aacgccttcg gcgttcagat taggacacca ccaatgagta ttccggtggc gcccgaacgt
      121 gtagaggccg ccattggtga gagtcggagt tgcgcgtgac cagaacgcgc tatagatccc
      181 ttgtaccgaa cgtgtcgccc tggcgcatgt cgcctgtatc gtaacctttt ctaaaccagc
      241 gcaggcgttg ttctgacgtg ccgtgagtaa aagagtctgg aactacccga ccggtagtcc
      301 gccgttgaat gcgatcgtcg ccgacagcgc tggccgcttg taacgcttct tcaacgtcgc
      361 ccgcctcgag caggccctgg ttctgaacgt aacgcgcaaa catcccggcg aagaagtcag
      421 cttgtagttc caaacggacg gagagttgat ttgcttctgc ttcggtcacg cgctgttgca
      481 tcgcgtgaac gcgatcggaa atgccgagca ggttctgaac gtgatggccc acttcatgcg
      541 cgatgacgta tgcctgcgcg aaatcgccgg gcgcgttgaa acgccggcgc agtgcttcgt
      601 aaaaagccaa atcgatgtaa accttttcgt cgccgggaca gtagaatgga ccaacagccg
      661 cccctgccat tccgcacgcc gactgcacct ggtctgtaaa aagcacgagc gttggttcac
      721 gatactggcg gccgttccgg cgaaagatct cgtgccaaac ttcttccgat ttaccaagaa
      781 ctaccgagac gaattgtttc agttcttctt cttcagcatt gcgaggacgc gaggtttgta
      841 cttcggccga acgatcgctc ggaagctgtt ccagcaactg ttgcggatca gcaccgaaca
      901 gcaacgcgat aatgatcacg atcactgttc cgagtccacc gccaaccgcg atcttcgatc
      961 cggttagccc acgccgatct tcaatgtttg tgctctgacg ttcccctcgc caacgcatgg
     1021 cttgactctc ctccgagtaa atgtttgcgg aggagagtcg caagactatg tgtcagcggg
     1081 acgatccttt aatggaattt tagtcacccg cgggattttt tctgcagttc ggaactaccg
     1141 accgtttacc tgggaccgca ggcgtctcgc ctgcacgatg acgaactggg cgctgccgca
     1201 agatgcaggc gagacgcctg cggtcccagg tagaaggcgc ccaagtttta gaactgcatc
     1261 actatccttg ttcgttgatt tctcgtgctt caagtttagc aagggtcgtc aactgggatg
     1321 cgcgcggaag gtgtgcagga gtccgagtgt aatcgccagt atcaccgggc cgagtatgat
     1381 ccccagcagg ccgaagatgc tgattccacc gaggacggaa aagaagacca ggagttcgtg
     1441 cagacgcgtc tggtttttca tccacttcgg tctgagaaaa ttgtcgaccg tgctgatgac
     1501 aagcgcgccc caaaccacga gcaggatccc tttcgtccag tgaccgttca tgatgagata
     1561 gatcgccaac ggcacccaca cgaggaacga tccgagaacc ggtatcatgc agacgaaggt
     1621 catcagcacc gcccatagca gcggcgaagg tatgccgaga acccaaaagg cgataccgcc
     1681 gagcagtcct tgcagtgcgg caatcacgac tatgccgtaa acgctcgcgc tgactacctc
     1741 gcgcgttcgt gcaatgatcg cttcgctctc ttttttttcg agtggcaagg ccgcgggtag
     1801 tttgctcacg atcctgtcgc cgtcgcggaa gaggtagtac atcgtgaaga tcacaaagaa
     1861 cgccttgacg ataccagtcg cgatgttgcc tgcgaggttt aaggacatgc tgaggaggaa
     1921 ttcgctggaa cgtttgagtt gctcgaccat gaactcctgt gaacgcaagg tgtcgacacc
     1981 aaaacgtccc tgaatccatc cggcaactct gccgatcacg gatgtctgcg ggtccatcag
     2041 tgaagagatc tgcgacggaa ggttggggag gacctttccc agttcgtcag cgacggcgat
     2101 cgtgaggagg gtcaaaggca gcaccgccag aagtacaacc agcaaacatg agatcagcga
     2161 actcagactg ggatttttta ttctgtctct gaggcgcagg tgaacggggt aaaagacaat
     2221 caccaaaacc actgcccacg ccagcacgcc gataaacggc cggagcatta accagcacag
     2281 ataaagtgcg agtgcggtcg ctaacaggac ggcgaaccag cgggcctgat cgatttggtc
     2341 tctcacgtgg cgctaatcgt agctattgat agcgactaag ggaaggcggc gatagcgaag
     2401 cggcggaagc aatgagcgcc tccggcgctc cgcaattgcg gcggcgctcc gtggtatccg
     2461 tggctccggc gcttgcgaat ccgagctccg acacttaaaa cttgctcctt gttttgtcag
     2521 cacgctcgtg gtctgtaaac tgacggcgca tgttgctgac catctcgacg acgtatcaac
     2581 ctgctacgga tctgggttac ctgctccaca aaaacccggc gcggttgcac acctttccgc
     2641 tttcgttcgg acaggcacac gtgttttatc cggaagcaac cgctgatcgt tgcaccgcgg
     2701 cgttgctgct cgacgtcgat ccggttggtc tcgtccgaaa tcgtcgcggt ccggccggcg
     2761 aaggcggcac gttggaccag tacgtcaacg accgaccgta cgtcgcgtca tcatttctca
     2821 gcgtcgcaat ctcgcgtgtg ttcgggagtg cgttgggcgg ccggagcaag aatcgcccgg
     2881 agttggccga aacaaaaatc ccgttagcgg caaagatctc ggtgttgcca tgccgtggcg
     2941 gtgaagactt cctccgtcgt ttgttcgagc cgctcggtta tcaagttatt gctcaacgtc
     3001 atccgctcga cgagaccttt cctgaatggg gcgtgagtcc gtatttcacc gtgcaactct
     3061 acggagtggt ccgcctgcaa gatctgttgg cacacatcta cgttctggtc ccggtgctgg
     3121 acaacgaaaa gcactattgg gtcggtgatg acgaggtaga gaaactgctg cgacgcggaa
     3181 gcggctggtt gagtgcgcat ccggaaaggg aagagatcac acggcgttat ctgaaatacc
     3241 gtcgcgatct cgcgcacgag gcgttggcca ggttgacgga agaagccccg gatgtcgatg
     3301 cggccgaagc gattcacgat agcgaagaag aggccgtcga acgcaaattg aacctgaatg
     3361 agcagcgact caacagcgtc gccgccgcac tcaagagcgc aggagcgaaa cgggtgttgg
     3421 atctgggctg tggagaaggc aagttgctgc gtgtgttatt tgcagatcgt tcctttgacg
     3481 agatcgtcgg cgtcgacgtg tcgcaccgcg ctctgcagtt cgctcatgaa cgtctgcgtt
     3541 tggagcgtat gcccgagcgg cagcgagagc ggctgaaact ggttcacgga tcgttgatgt
     3601 accgcgaccg gcggctctcc ggctttgatg ccgcggccgt cgttgaagtt atcgaacacc
     3661 tggaccaacc gcgactggca gcctttgaac gagtcttgtt cgagtttgcg aaaccgaaga
     3721 cgatagtgtt gacgactccg aacatcgagt acaacgcacg tttcgaaaac ctcccggcgg
     3781 gaaagtttcg ccacaaggat caccgcttcg agtggactcg cgcggagttt caccactggg
     3841 cgcgcgcgat agcggatcga tttggctatg cggttcgctt tctgccggtt ggtccggaag
     3901 acagcgcgct cggcgcgcca acgcagatgg cagtttttaa aagggaacag gctgcttgaa
     3961 gacgattacg attccagaac tcgcgctcgt agtgctcatt ggcccgtcag gttgcggtaa
     4021 atcgacgttt gccctgagtc atttcaaacc caccgaggtg ttgtcgtccg acttctgccg
     4081 cgcgttggtt tctgacgacg agaataatca ggcagccacc aaagatgctt ttgaagtgct
     4141 gcacttcatc gcggccaagc gcctcgcggc gggaaagttc agcgttgtcg atgcgacaaa
     4201 cgtgcagccc gaagcacgca aaccactcgt agcgcttgcg cgcgcgtatc acgtcattcc
     4261 cgtcgctatg gtgctcaacc tgcccgatcg ggtttgtcag gatagaaacc gtctgagatc
     4321 ggatcgacag ttcggtgcgc atgtgattcg acagcagagt cagcaactgc gaaggtccat
     4381 ccgcaatctg aagcgcgaag gctttcgtca catttatgaa ttctcttcgc aggcggaagt
     4441 agacgaagtc gtaatcgaac gccagccgct ttggaataac cgtaagcacg agcatggacc
     4501 tttcgacatc
//
LOCUS       DASTEY010000024         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1088164, whole genome shotgun sequence.
ACCESSION   DASTEY010000024 DASTEY010000000
VERSION     DASTEY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088164"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFO72_00410"
     CDS             complement(<1..609)
                     /locus_tag="VFO72_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477772.1"
                     /translation="MQMIVTISIIAVVSVFGVLGITNARAEFRLQNSARLFATYVEKA
                     RVDAIRRHAPVGQESAIETFGPGTSNYTVTMDFGTGALETRTFQLENGLVFSTAAMRL
                     TFDWRGRIAQAWVFQIFSSYLQRSMPVDVSGSGDITVGEQHFPDQLIPAVEVAQVTGD
                     VDPDPNPSPSLSPTEEPTPPIEETPTEDEVDSPTPTPTPTPTP"
     gene            997..>2180
                     /locus_tag="VFO72_00415"
     CDS             997..>2180
                     /locus_tag="VFO72_00415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9477773.1"
                     /translation="MADILLVEDKDSLRRVLRLTLENAGYSVTEAIDARAALNEIANS
                     RHRLVLTDLRMPNGSGLDVLRSARAADPDVPVIVMTAFGSIDEAVQAMKDGAHDFLQK
                     PVDSNHLLLLIERALEQARLRTENVLLREEWSRRYGFPRILGESETLKRAVGETQRVA
                     QTEATVLLLGESGTGKELFARAVHHLSARRDHPFVAINCAAIPETLIENELFGHERGA
                     FTGASDRRQGKFELASGGTVFLDEIGELPLAVQGKLLRAIEEKVVDRIGGRAPVAVDV
                     RVVAATNKDLKAAVENGQFRGDLFFRLAVFPIEVPPLRDRGDDIVLLSQHFASEIGRE
                     LRGREAQLSSESIAALKQHHWPGNVRELENAIERACIISDSLLLQPSDLGLGPSNAQG
                     TET"
BASE COUNT          447 a          596 c          650 g          487 t
ORIGIN      
        1 cggcgtcggc gtgggggtgg gggtgggggt tggtgaatct acttcatctt cggtaggtgt
       61 ttcttcaatc gggggagtag gttcttctgt tggagagagt gacggtgacg gattaggatc
      121 aggatcaaca tcgcccgtga cctgggccac ttcaactgcc ggaatcaact gatcgggaaa
      181 atgctgttcg ccgacggtga tatcgccgga gccagaaacg tcaaccggca tactacgctg
      241 gagatacgaa ctgaatatct ggaacaccca ggcttgagca attctgccgc gccagtcaaa
      301 tgtcaacctc atggcagccg tggaaaaaac gagtccattt tcgagctgga aggtgcgtgt
      361 ctccaaagcg ccagtaccaa aatccatggt aaccgtgtaa ttggaggtgc ctggaccaaa
      421 tgtttcgatc gccgattcct ggcctaccgg agcatgacgg cgaatggcat caacgcgggc
      481 cttctcaacg taggtggcga agagccgggc ggagttctga agccggaatt cggcgcgtgc
      541 gtttgtaata ccgagcactc caaagacgga gacgacagcg atgatcgata tagtgacgat
      601 catctgcata gttgtgaatc cgcgatgttg cgtggctgct cctggtttgc ggttcattgg
      661 ggcatctacc tccggcccat ccggggcaac aatcgttccg tcgcagtttg tttgggtttt
      721 tcgaggtttt ggtggaggaa caaatggagg ctgttacctt aagttacatg gcgaggaagc
      781 gggcttccta tttgaacctg cggagcaggt ttcattctgg acgccgagcc cgcgaaagcg
      841 ggcggcagcg ctgtcggccg cttcgcgggc ttacaattgt tttctcattt gctacctggg
      901 gcttgcgccc caggctttat tctttcgtcc gcttcgcgga ctctaaatct agagatctct
      961 tgacactcac cagcgaaaaa tcaattattg gcgccagtgg ctgatatcct acttgtcgaa
     1021 gacaaagaca gtctgcgtcg cgtgctgcga ttgaccctcg agaatgccgg atacagcgta
     1081 acggaagcga ttgatgcccg cgcggcgctc aacgagatcg cgaattcccg tcaccgcctt
     1141 gtcctcactg acctgcgcat gccaaacggt tccggcctgg acgtgctgcg ttcagcgcgc
     1201 gccgccgacc cggacgtacc cgtcatcgtc atgactgcct tcggctctat cgacgaagcc
     1261 gtgcaggcga tgaaagacgg cgcgcacgac tttctgcaaa agccggtcga ttcaaatcat
     1321 ctcctgttgc tgatcgagcg cgcgctcgaa caagccagac tgcgaaccga gaacgttctg
     1381 ttacgcgaag agtggtcacg ccgctatggc tttccgcgca tcctcggcga gtcggaaacg
     1441 ctgaaacgcg ccgttggcga aactcagcgt gtggcccaaa ccgaggccac agtcttactg
     1501 ctcggcgaat cgggcaccgg caaagagctc ttcgcgcgcg cggtccatca tctttccgct
     1561 cgccgcgatc atcctttcgt cgccatcaac tgcgccgcca ttcctgaaac gctgatcgaa
     1621 aacgagctct ttgggcacga gcgtggtgct tttacgggcg cgagcgatcg acgtcagggt
     1681 aagtttgaac ttgcttccgg cggcaccgta tttcttgacg agattggaga gttgcctctg
     1741 gccgtccagg gaaaactctt gcgtgcaatc gaggagaaag tggtggatcg tatcggcgga
     1801 cgtgctccgg ttgcggtcga tgtgcgtgtc gtcgctgcga caaataaaga cttgaaagca
     1861 gcggtcgaga acggtcagtt tcgtggcgat ctgtttttcc gtctcgcggt gtttccgatt
     1921 gaggtgccgc cgttgcgcga ccgcggcgac gacatcgtgc tgctgtcaca gcatttcgcc
     1981 tcagagattg ggcgcgagtt gcgcgggcgt gaagcgcagc taagttccga atctattgcg
     2041 gcactgaaac agcatcactg gcccggcaac gttcgcgagc tcgagaacgc aatcgagcgt
     2101 gcctgcatca tctccgattc gctgttactt cagccttcgg acctggggct tggaccatca
     2161 aacgcgcaag gcaccgaaac
//
LOCUS       DASTEY010000025         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_33325, whole genome shotgun sequence.
ACCESSION   DASTEY010000025 DASTEY010000000
VERSION     DASTEY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33325"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VFO72_00420"
     CDS             complement(<1..1449)
                     /locus_tag="VFO72_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9477774.1"
                     /translation="MSATLGDDDNAQPREGREWATTLDPEEAPSVAEPEAAPVAEEIR
                     EPSGSLETTVVPPEELAESSPRISSNSLGGSFTDNVQAPGKTSIRESTTGGHRPSVKQ
                     LAQNTVLNGRYEIVRRIGGGGMGAVYLAKDRNLGDAPRAVKEMVESHLDPAQHEKAIG
                     DFKRESLLLTSLEHPSIPTIYDYFYDEPRFYLVMKYISGGDLASRMRAAVGGRIDEKT
                     VTDWGMQVADVLDYLHSRPKPIIYRDLKPANLMIDGNTGRVMLIDFGIARWVTQQEKG
                     VTAVGTMGYAPPELFSGRVQPASDVYSLGATMFHLLTGADPQDNPLLIFDFSKNPRPR
                     QIAPAISSEMEQILMRAVEYKPDDRFRTAREMRNVLADHLEKLIEGKVSYGMPAPALG
                     QETVQVQTVYCGFCGGRIAADDVFCAHCGARQPLAVVGSSALLHTVRPTAKLVVTGTT
                     ELDASFILQKDSNLLGRTDPHSNIFPEIDLSRF"
     gene            complement(1615..1965)
                     /locus_tag="VFO72_00425"
     CDS             complement(1615..1965)
                     /locus_tag="VFO72_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9477775.1"
                     /translation="MRTTAKPHARLVIEKGRSTGKQFMLSDAEAQIGRWDADGGIFPD
                     VDLDTDDPEAKVSRRHARITLSNGQYFLEDLGSTNGTFINRGKRLPPGQRQALCDGDE
                     IIVGKTFLRFHIVK"
     gene            complement(2228..>2469)
                     /locus_tag="VFO72_00430"
     CDS             complement(2228..>2469)
                     /locus_tag="VFO72_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477776.1"
                     /translation="RVAITLMTTALMSTTPTMTPIIIFFFLLNAISGVPSTWGLMLEE
                     VAFTWKLIKQFHVRANQQGQHPNAETRILLTKHRH"
BASE COUNT          535 a          703 c          660 g          571 t
ORIGIN      
        1 aaatcttgaa agatcgatct cgggaaagat attcgagtgc ggatcggtgc gcccgagcag
       61 attgctatct ttctgcagga tgaacgacgc gtcgagctca gtggttccag taacaacgag
      121 tttcgcagtc ggtctcacag tgtgcagcaa cgcactcgat cccactaccg cgagcggttg
      181 gcgcgcgcca cagtgcgcac aaaacacatc gtcagcagca atgcgtccac cgcaaaaacc
      241 gcaatagact gtttgtacct gaaccgtctc ctgacccagc gcaggcgcgg gcattccgta
      301 gctcactttc ccctcgatca acttttcgag gtggtcggcg agcacgtttc tcatctcgcg
      361 cgcggtgcgg aaacgatcgt cgggtttgta ctccaccgcc cgcatcagga tctgttccat
      421 ctcactcgag atggccggcg cgatctggcg cggtctcgga ttcttcgaga agtcgaatat
      481 caggagcgga ttgtcctgtg gatcggctcc ggtcagcagg tgaaacatcg tcgcgccgag
      541 actataaacg tccgaagcag gctgcactcg cccgctgaag agctccggcg gcgcatagcc
      601 catcgtgcca accgcggtca cgcccttctc ctgctgcgta acccagcgag cgataccgaa
      661 atcgatcagc atcactcgac ccgtgttgcc gtcgatcatc aggttcgccg gcttcagatc
      721 gcgatagatg atcggcttgg gtctcgagtg cagatagtcg agcacgtcag ccacctgcat
      781 gccccaatcg gtgactgtct tctcgtctat cctgccgccg actgccgcac gcattcgcga
      841 agcgagatcg ccacccgaga tgtatttcat cacgaggtag aaacgaggct catcgtaaaa
      901 gtagtcgtag attgtcggta tcgacggatg ctcgagcgac gttaacaaca gcgactcgcg
      961 tttgaagtct ccaatcgctt tctcgtgctg cgccggatcc aggtgcgact caaccatctc
     1021 tttgacggcg cgcggagcat cgccgaggtt acgatccttc gccaggtaaa cagcacccat
     1081 accgccgccg ccgatccgtc gcacgatctc atagcgaccg ttcaaaacag tgttttgagc
     1141 gagctgcttg actgatgggc gatgaccacc ggtcgtgctt tctctaatcg aggttttacc
     1201 gggcgcctgg acgttatccg taaacgatcc gccgagtgaa ttcgaagata tccgaggcga
     1261 cgactcggct agctcttcag gtgggacaac agtagtttcg agactgccgg aaggctcccg
     1321 aatctcctct gctaccggtg ctgcttctgg ctctgcgaca ctcggtgctt cctcgggatc
     1381 gagtgtagtc gcccactcgc ggccttcgcg tggctgcgcg ttgtcatcgt ctcccaacgt
     1441 ggcagacatg gaatcaaact ctactgaatt ctgtattagc tgcgcgccgc aattgccgca
     1501 atagtgttca ttatcgcgta cgcgggcgcc gcaggagtta cattcagtca tcgataacga
     1561 ataaccagtc tttcagatta aacgcggcta actagaagac gtcgtagcag gctactattt
     1621 cacaatgtgg aagcgcagaa atgtctttcc gacgataatt tcatcaccat cgcataatgc
     1681 ctgacgctgt cctgggggta atcgcttgcc gcgattgatg aacgtaccgt ttgtcgatcc
     1741 tagatcctcg agaaaatatt ggccgttact gagagtgatg cgcgcgtgac gtcgcgatac
     1801 cttggcttcg ggatcatccg tgtccagatc gacgtccggg aagatcccgc catcggcgtc
     1861 ccaacgcccg atctgtgcct ctgcatcact caacataaac tgctttccgg tcgagcggcc
     1921 cttttcgata acgagacggg catgcggttt ggcagtggtt ctcaaagctg aaaaactcaa
     1981 gttggcagaa acgcgaccac cggcgaattc cggatgcggt ccgggctgcg gcgcgtgatt
     2041 gccgttgcct ccgtgtccag gcttcctttg aaccgggttt ggacggccat tcgggcgcaa
     2101 aggtgcgcca cactcgtcac aatattgcga gccatccaga ttctctgttt tgcacctgtc
     2161 acaggtaatc atcgtgttac aaaagcaagt cattttaccg cgaacgcggc tgccacgcca
     2221 acgcatctta atggcggtgt tttgttaata aaatccgcgt ttcggcgtta ggatgttggc
     2281 cctgttgatt tgctcttaca tgaaactgtt tgataagttt ccacgtaaaa gctacttctt
     2341 caagcattag gccccaggta gaaggaaccc cagagatggc attgagtaag aaaaagaaga
     2401 ttataattgg cgtcatagtg ggggttgtgc tcatcagcgc tgtcgtcatc agcgtaattg
     2461 caacccgca
//
LOCUS       DASTEY010000026         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_824507, whole genome shotgun sequence.
ACCESSION   DASTEY010000026 DASTEY010000000
VERSION     DASTEY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824507"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="pepT"
                     /locus_tag="VFO72_00435"
     CDS             <1..705
                     /gene="pepT"
                     /locus_tag="VFO72_00435"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripeptide aminopeptidase PepT"
                     /protein_id="HET9477777.1"
                     /translation="EVGACIEKFDVNGFGAKVAYTVDGGALGEISEETWSARTATVSF
                     QGKSTHPGSAKNIMINAMYALADYLSRFPKDMLPETTEGRVGFVHPYAGTVDVETSSL
                     KVLLRDFDISGLEAKEKLLRDMLAQTAAKFPEVKITIDVKENYKNMKEVLKNHPELTN
                     NAMEAARRAGLKPFLKPIRGGTDGARLTFRGLPTPNLFTGGSNFHGKLEFNSRRGLEK
                     STETLVHLVQIFAEKS"
     gene            771..1694
                     /gene="era"
                     /locus_tag="VFO72_00440"
     CDS             771..1694
                     /gene="era"
                     /locus_tag="VFO72_00440"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9477778.1"
                     /translation="MSSTEHKEQSTNDKAHTGYVALIGRPNAGKSTLLNRLVGQKIAA
                     VSDKPQTTRFRIQGVITKPEGQIVLVDTPGVHQPGYELNRRMMAAVQDALLGVDLVCL
                     IRDASVTTGNGDRFVLDLVKRSEKPALLLLNKIDKLEDKAALLPLMDWYRNEYDWREI
                     IPISALKDEMTDVLVDSCLRHLPEGEPIFDEDELTDQTLRVLVSEIVREKLLQVTGDE
                     IPYVTAVATERFEEVREDFTRINCVIVVERPSQKKIIIGRGASRLKEIGIRARQEIEA
                     LLGHRAHLELFVKVEEDWRNSPRLLNEFGLQ"
     gene            1700..2539
                     /gene="prmA"
                     /locus_tag="VFO72_00445"
     CDS             1700..2539
                     /gene="prmA"
                     /locus_tag="VFO72_00445"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9477779.1"
                     /translation="MKEKWYALDVDIDPQAREAVGYALMEAGAAGTTDDTERTRITGY
                     FEGAPDRERVRKELFEALRIYELPSSSVSDMNVREVEQRDWLEEWKKNWQPVAVGRFI
                     IAPPWVELDETDDRCVIRIEPGMAFGTGTHETTRLCLGAMQSYFQGGSFLDVGTGTGI
                     LAIAAATCAPGAHVEACDIDPEAIEIARENAVVNGVAGRISFRAGSVDEQTSSADFIC
                     ANLTAQVIAKLLPTLVALSCGRLVLSGILETQIKEIIDQLAQLAITDVEIRQEGEWVA
                     IIV"
     gene            2544..3269
                     /locus_tag="VFO72_00450"
     CDS             2544..3269
                     /locus_tag="VFO72_00450"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET9477780.1"
                     /translation="MTRRRFFAPPSAFHPGKRSVTLAADEARHLREVLRLKPGDEVSV
                     FDGEGKEFRARVAQARREFAELALDEEIPPARPESPLQITLAVALLKGEKFDLVVQKA
                     TELGVNKIVPLTTRYADIKLRDEGDASKRVARWQRIALEAAKQSGRAVVPEISLPATL
                     ALILSETCLLFSEKGGHGLTEIATDKITAIIGSEGGWSNEELDEARTAGAHIVTLGGR
                     ILRAETAAITAAALLQHRFGDLK"
     gene            3382..3657
                     /locus_tag="VFO72_00455"
     CDS             3382..3657
                     /locus_tag="VFO72_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477781.1"
                     /translation="MGISKKDAVEKARKDLAQRLNVDASEISEQAVEDSDFPDMSLGA
                     AEDGEMSGQMITRGWRIRLGASGKTYEYRADKNQVRLYKYQGKNYRI"
     gene            3667..5073
                     /locus_tag="VFO72_00460"
     CDS             3667..5073
                     /locus_tag="VFO72_00460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET9477782.1"
                     /translation="MNRNNFLIHLALAVVLILIAAFAGQLNRWRKQWKSTVRIIPAAE
                     KKRVSTSGEIALAEGRVLGRPEVLVKFKPGVSLETIEELTSRFHDRVEDRIEHAPGWS
                     SIDDLDNADAYQTVAQYLALPEVEYAEPNMEIKLDALDASLLPVFPADPRFNDQWALA
                     NSGQRGGKEGADISATMAWATTTGSDKVVVAVLDSGVDYTHEDLAPNIWVRPASLGPY
                     HDNELGMVDDVSGYNAIENSSDPMDENGHGTHCAGIIGAEGSNNIGISGVNWKVRIMP
                     LKFMNAGGFGTTKDAIEAINYVIDRKKAGVNVRIISASWGGTQKSRALEEVIRKAYEN
                     DILFVAASGNASTNNDRNPHYPSSYNVPNVVSVAALNRNDQLASFSNYGVKSVAIAAP
                     GADILSTWLGNEYEEKSGTSMATPVVSGVAALILAENPSISVDELKKRLLAAVDPLPA
                     LNGKIATGGRVNAAKAVE"
BASE COUNT         1210 a         1261 c         1592 g         1034 t
ORIGIN      
        1 gaagtcggcg cctgtattga gaagtttgac gtgaacggtt ttggcgcgaa ggtcgcttac
       61 acagttgacg gcggcgcgtt gggtgagatc agtgaagaga cttggagcgc gcgcacagca
      121 acggtttctt ttcaaggcaa gagcacgcat cccggcagcg caaagaacat catgataaat
      181 gcgatgtatg cgctggccga ctacctgagt cgctttccga aagacatgct gccggaaacg
      241 actgaaggac gcgtcggatt cgttcatcct tacgcgggaa cagtggacgt cgaaacttct
      301 agtttgaagg tcctgcttcg cgacttcgat atcagtggac ttgaagcaaa ggagaaactg
      361 ctgcgcgata tgctggccca gacggcggcg aagtttccgg aagtgaagat cacgatcgac
      421 gtaaaagaaa actacaagaa catgaaggaa gtgctgaaga accatccgga gttgacgaac
      481 aacgcgatgg aagcggcgcg ccgcgcaggt ctgaagcctt ttttgaaacc gattcgcggt
      541 ggcaccgatg gcgccagact gacgtttcga gggttgccaa caccgaatct tttcaccggt
      601 ggttcaaact tccacggcaa gctggagttt aattcgcgcc gcggactcga gaagagcacg
      661 gagacgttgg tccacctggt ccaaattttt gcggagaaaa gttaatgttt gaagctttgt
      721 acctggtgct ttgtactttg tattgcggaa cgggtctgcc aaagtaggtt atgtcaagca
      781 cggagcacaa agaacaaagt actaacgaca aagctcatac tggttacgtc gcgttaattg
      841 gacgacctaa cgccggcaag tcgactctgc tcaaccggct cgtcggccag aagatcgctg
      901 ccgtctccga caagccgcaa acaacgcgct tccgcataca gggcgtaatc accaaacccg
      961 agggccagat cgtgttggtg gatacgcctg gcgtgcatca gccgggttac gaactcaacc
     1021 ggcgcatgat ggctgcggtc caggatgcgc tactcggcgt cgacctcgtc tgtttgattc
     1081 gtgatgcatc cgtcacgacc ggcaacggcg atcgcttcgt gctcgacctc gtgaagcgat
     1141 cggaaaaacc cgcactcctg ctgctcaaca agatcgacaa actcgaagat aaagcggcgc
     1201 tcctgccgct gatggattgg tatcgaaacg agtacgactg gcgtgagatc attccgattt
     1261 cggcgttgaa agacgagatg acggacgtct tggtcgacag ttgtttgcgc catctgcctg
     1321 aaggcgagcc aatctttgat gaagacgagc tgaccgatca aactttgcgc gtgctggtgt
     1381 cggagatcgt gcgcgagaag ctgttacagg taactggcga tgagattccg tacgtgaccg
     1441 cggttgcgac tgagcggttt gaagaggtgc gcgaagattt cacgcggatc aattgcgtga
     1501 tcgtggtcga gcggccgtcg caaaagaaga tcatcattgg ccgtggcgcc agccggctga
     1561 aggagatcgg cattcgtgcg cggcaagaga tcgaagcgct gttgggccac cgcgcgcatc
     1621 tcgaactgtt tgtgaaagtc gaagaagact ggcgcaacag tccgcgactg ctgaatgagt
     1681 ttggtctcca atgagcgcga tgaaagaaaa atggtacgcg ttggatgttg atattgaccc
     1741 gcaggcgcgc gaggccgttg gttatgcttt gatggaagcg ggagcggctg ggaccacgga
     1801 tgacacggag agaacaagga ttaccggcta ttttgaggga gcgccggatc gcgagcgagt
     1861 gcgaaaggaa ctgtttgagg cactgcggat ctatgagctg ccgtcttcgt ctgtgagcga
     1921 catgaatgtg cgcgaggtcg agcaacggga ctggctcgaa gagtggaaga agaactggca
     1981 gcccgttgca gtggggcgtt tcatcatcgc gccgccatgg gtcgagctgg atgagacgga
     2041 cgaccgctgt gtcattcgca tagaaccagg aatggctttt gggacgggaa ctcacgaaac
     2101 aactcgactc tgtctcggcg ccatgcaaag ctatttccaa ggtggcagtt ttctcgacgt
     2161 cggcacaggc acggggattc tggcgatcgc ggcggcgacg tgcgcgcccg gagcgcacgt
     2221 cgaagcgtgc gacatcgatc ctgaagcgat cgagatcgca cgtgaaaatg ccgtggtgaa
     2281 cggcgtagca ggcaggatta gttttcgtgc cggttcggtc gacgaacaaa ccagctcggc
     2341 ggattttatt tgtgcgaact tgacggcgca ggtgatcgcg aaactgttgc cgacgctggt
     2401 tgcgttaagt tgcgggaggt tggttttgtc ggggatttta gaaacacaga tcaaagagat
     2461 aattgaccag ctggcgcaac tggcgattac tgatgttgag atcaggcaag agggcgagtg
     2521 ggtagcgatc atcgtttgaa ctcatgacgc gtcgccgctt ctttgcgcct ccgagcgctt
     2581 tccatcctgg caaacgcagc gtgacgctgg ctgctgatga agcgcgtcat ttgcgcgaag
     2641 tgttgcgttt gaaaccgggc gacgaggtat cggtgttcga cggcgaaggc aaagagtttc
     2701 gcgcgcgtgt ggcccaggcg aggcgtgagt ttgcggaact cgctcttgat gaagagatcc
     2761 cgccggcgcg gcctgaatcg cctttgcaga tcacgctggc agtggcgttg ttgaaaggtg
     2821 agaagtttga tctcgtggtg caaaaagcga ccgaactggg tgtcaataag atcgtgccgc
     2881 tgacgacacg ttacgctgac attaagctgc gcgatgaagg cgacgcgagc aagcgcgttg
     2941 cgcggtggca gcggatcgcg ttggaagcag caaagcagtc cggcagagct gttgtgccgg
     3001 agatcagtct gcccgctacg ttggctttaa ttttaagcga gacgtgtctg ttgttctcag
     3061 aaaaaggggg acacggatta acagagattg cgacggacaa aatcactgcg atcatcggat
     3121 ccgaaggtgg ctggtctaac gaggaactcg acgaggcacg cactgccggc gctcacatcg
     3181 tgaccctcgg cggccgcatc cttcgcgccg aaacggcagc aatcacagcc gccgccctgc
     3241 tccaacaccg cttcggcgat ctcaaatgac cgtgcggaca ttgaccacag cctgcgacag
     3301 gctcaaaatg gcaacacctc cccgggaaca aagaaagctg aaaggtcgtt tgagtcctca
     3361 gcggcaaggg aaccgttcac aatgggaatc agcaaaaaag acgcggtcga aaaggctcgc
     3421 aaggatctgg cgcagcggct aaacgtcgac gcaagtgaga tcagcgagca ggcagttgaa
     3481 gactcagact ttcccgatat gtctctcggc gccgccgaag acggcgagat gagcggccag
     3541 atgatcacgc ggggttggcg catcaggctc ggcgcaagcg gcaaaactta cgaataccga
     3601 gccgacaaga atcaggtgcg tctctacaaa taccagggca agaactatcg catttgaggt
     3661 accgcaatga accgcaataa ctttcttatt cacctcgcac tcgcagtcgt tctcattctg
     3721 atcgcagcct ttgccggcca gttgaaccgc tggcgcaaac agtggaagtc gacggtgcgg
     3781 atcattcccg ccgcggagaa gaagcgtgtg tccacgtccg gggagatcgc tctcgcggaa
     3841 ggacgcgttt tgggccggcc ggaagtgctg gtgaagttca aaccgggcgt gagtctcgaa
     3901 acgatcgaag agttgacttc gcgtttccac gaccgagttg aagaccggat tgagcatgct
     3961 ccgggctgga gttcgattga cgacctcgac aacgccgacg cgtatcaaac cgtggcgcag
     4021 tatctcgcgc tgcctgaagt tgagtacgct gaaccaaaca tggagatcaa actcgatgcg
     4081 cttgatgcgt cgctgctgcc ggtgtttcca gcggacccac gttttaacga tcagtgggca
     4141 ctcgcaaatt cagggcagcg aggtggaaag gaaggcgctg acataagcgc gacgatggcc
     4201 tgggccacta ctacgggcag tgacaaagtg gtcgttgcgg tgttggacag cggagttgat
     4261 tacacgcacg aagatctcgc gccaaacatt tgggtgagac ctgcaagcct cgggccctat
     4321 cacgataacg aactcggcat ggtcgacgac gtgagcggtt acaacgccat cgagaacagt
     4381 tcggatccga tggacgagaa cggtcacggc acgcactgcg ccggcatcat cggtgccgaa
     4441 ggcagcaaca atatcggtat cagcggtgtg aactggaaag ttcgcatcat gccgttgaag
     4501 tttatgaatg ccggtggttt tggcacgacg aaagacgcga tcgaggcgat taattacgtg
     4561 atcgatcgga agaaagcggg cgtgaatgtt cgcatcatca gcgcgagctg gggtggaacg
     4621 caaaaatcac gcgcgcttga agaggtcatt cgcaaggctt acgagaacga tattctgttt
     4681 gtagctgcga gtggaaatgc cagcacaaac aacgatcgca atccgcatta tccgtcgagc
     4741 tacaacgtgc cgaacgtagt cagcgttgcg gcgcttaatc ggaacgatca gttagcgtcg
     4801 ttttcaaact acggcgtgaa gagtgtggcg atagcggcgc ctggtgcgga tattttaagc
     4861 acctggctcg ggaacgagta cgaagagaag tctggcactt cgatggctac gccggttgtg
     4921 tctggtgttg cggcgttgat tctcgccgag aatcctagta tctcggtgga tgaactcaag
     4981 aaacggctgc tggcggcagt tgatccgttg cctgcgttga acgggaagat tgccaccgga
     5041 gggcgtgtta acgccgctaa agccgtggag taaaaaatct ttgcgatttg agcgcga
//
LOCUS       DASTEY010000027         5333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_758606, whole genome shotgun sequence.
ACCESSION   DASTEY010000027 DASTEY010000000
VERSION     DASTEY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758606"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VFO72_00465"
     CDS             complement(<1..1319)
                     /locus_tag="VFO72_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9477783.1"
                     /translation="MCWVSVAVAQSPANQDYLLGRKAETSGDIAAATTSYQSVISGNS
                     ILKEYALWRLARIARATGDLVLEREQLQRLIATAPNSLLYETAALRLAESFFESRDFE
                     AAATSAKAIASSKNVAVARESAALMGSAHVRAGKPAEARDVFTKLVMQMPDASRPDDF
                     ALLAVRELDALDKVSATANLSEAEHLLHASVYQFNRDFAGARAHYQALIDRFPQSGTL
                     PNAIVQLGRGYYLEHKYDDAIKAFQKVFDAYPQSTAAREAVGWLASSYVRMKRTDDAV
                     TAYKLLIDRFPDNPNPERAYLNIIDVLHEAGRHADALNWVQQTRARFKNDLGGTLALF
                     AQLRIHLAQTSWAAVVRDGDELSKFSDLGGTRVPGGTSATEVAFLRAYALEQLGRTEE
                     AVAGYLTIPDGRNEYYGARATQRLLSLANGEKSKPLVQMRLNALSNKA"
     gene            1395..2348
                     /locus_tag="VFO72_00470"
     CDS             1395..2348
                     /locus_tag="VFO72_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET9477784.1"
                     /translation="MITEKTEARPLEGAVVAARIKQEVAAEVEKLVQTLGVRPCLAAV
                     RVGDDPASAVYVRNKIKACEEVGIGSEHHSLPESISTADLLNLVNSLNARDDVDGILT
                     QLPLPRGIDETAIIEAIDPRKDVDGFHPVSAGKLALGRPVFVPCTPAGIIELLDYNGV
                     EIRGANACVVGRSQIVGRPMAQLLLQRDATVTICHSRTRDLPAVTRQADLLVVAIGRA
                     GMIGRGHIKPGAVVIDVGMNRVSDANEVRRLFGEAAEKRLEIVAKRGSTLVGDVNPAE
                     ASEVAGMLTPVPGGVGLLTVAMLMKNTLKAAKLRRAQAQNP"
     gene            2357..2968
                     /gene="coaE"
                     /locus_tag="VFO72_00475"
     CDS             2357..2968
                     /gene="coaE"
                     /locus_tag="VFO72_00475"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET9477785.1"
                     /translation="MLRVGLTGSIAVGKSYVASVFAELGCHVLDADQTAREVVLPGSA
                     GLAAVAAAFGSDVLNSDGALNRQRLGEIVFAHEDKRQQLNQILHPFIIARQDEIMREW
                     EREDPDGIGVIDAALMIESGGYRRFDKLIVVHCRPEVQLERLMLRNNLSREEAQARID
                     SQMPQAEKQKYADFLIDTSDGFEPTRKRTNEVYNELVKLTGKR"
     gene            2969..4630
                     /locus_tag="VFO72_00480"
     CDS             2969..4630
                     /locus_tag="VFO72_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9477786.1"
                     /translation="MKTKTVTSFRPRRLRQLRWTQVYALSLVFCLVLTGCFNEEPSTY
                     YGKIVPPRAQEFRWSDGGLPQTFDPAFAAAPPDTDAVRALFEGLTDYDPQTLAPVPGV
                     AQRWESSQDGRVWTFYLRDNARWSNGESVTAGDFVRSWQRTLKIGPLAPHTELLNNIE
                     GATATPPPVNATEAAPHFGAKAIGEHVLQVTLQQADDSFPALVAHPVFRPVKLADAEN
                     TKPIESHELISNGAFLLSAAEEDRVRLERARTYWDGESVSLERVTFVNAANPEDALSA
                     YHQGEIDAVTNAAFEPLALKLLAPYKDFRRSTFGALTYYVFNVDREPFDDVRVREALA
                     LAIDRERVSRDDLRGATEPAGRFLPDAMSGEKPVVEKTELLDHDVNKARELLAEAGYP
                     DGDGFPVMRLLINRNEQQRTVAQSIAAMWRATLNIQTEIVIKSWDEYEAAIQAGDYDV
                     VRRGLVMQTTSELTNIAMLFGRDSHPAPVQPPAGLANPPAPEPQPALIETEAQALKEL
                     KAMPIYFASSYSLVKPYVSGFDRNVLDAPSLKRTRLDTNWSEQGR"
     gene            4627..5208
                     /locus_tag="VFO72_00485"
     CDS             4627..5208
                     /locus_tag="VFO72_00485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9477787.1"
                     /translation="MKASDVISSGGVIAFRTDTFYGLGADPFNASAVRRIRELKGRED
                     DNPILLLISDRPQVERLLVECSHEFNIAAQELWPGPLTIVGKAAATLPEEITAGTGTV
                     GVRLPADKSVRDFVRECGGVLTATSANPSGREPARSAQEAAAYFPTGLDLIVEGGEVN
                     ATKPSTVLDVSSFPPRVVREGAIARNVIEQLFA"
BASE COUNT         1121 a         1507 c         1611 g         1094 t
ORIGIN      
        1 gccttgttgg ataacgcgtt gagccgcatc tgcacgagtg gcttcgactt ctcgccgttc
       61 gccaacgaaa gcagacgttg tgttgctcgc gcgccgtagt attcgttacg cccgtcggga
      121 atcgtcagat agcccgctac agcctcttcc gttcgcccga gttgttccaa cgcgtaagca
      181 cgcaggaaag cgacctccgt cgcgctggtg ccgccgggca cgcgcgtgcc accgagatcg
      241 gaaaacttcg acagctcatc gccatcgcgc acaacggcag cccaacttgt ctgcgccaga
      301 tgaatccgca gttgcgcgaa cagcgccagc gtgccgccga gatcgttttt gaatcgcgcg
      361 cgcgtctgtt gcacccagtt caaagcatct gcgtgacgcc cggcctcgtg cagtacgtcg
      421 atgatgttca agtaggcgcg ctcaggattt ggattatcgg gaaaacgatc aatgaggagt
      481 ttgtaagcgg tgaccgcgtc gtccgtgcgc ttcatgcgca catagctgga cgccagccaa
      541 ccgacagctt cgcgggcggc agtcgattgc ggatacgcgt caaagacttt ctgaaaagct
      601 ttaatcgcgt cgtcgtattt gtgttcgaga tagtagccgc ggccgagttg tacaatcgca
      661 tttggcaatg tgccgctttg tggaaaccgg tcgatgagcg cctggtaatg cgcgcgtgca
      721 ccggcaaagt cgcgattgaa ctggtagacc gacgcatgca gcagatgttc tgcttcgctc
      781 aggtttgcgg tcgcagacac tttatccagc gcgtcaagct cgcgcaccgc cagcagcgcg
      841 aagtcgtcag gacgcgacgc atcgggcatc tgcatcacga gcttcgtaaa aacgtcgcgc
      901 gcttcggccg gtttgcccgc gcgtacgtgc gcagagccca tcaacgcggc gctctcacgc
      961 gccaccgcga cgttcttcga tgacgcaatc gcctttgcac tcgttgccgc cgcctcgaaa
     1021 tcccggcttt caaagaaact ctcggcaagt cgcagcgctg ccgtttcgta gagcagacta
     1081 ttgggagctg tggcaattag tcgttgcagt tgttcacgtt cgaggacaag atcgccagtc
     1141 gcgcgagcga ttcgcgccaa acgccacaaa gcgtattcct tgagaatcga attgcccgag
     1201 atgactgatt ggtaactggt ggtcgcagcg gcgatgtcgc ctgaggtttc ggctttgcgt
     1261 ccgagtagat agtcctggtt agccggcgac tgggccacgg caaccgaaac ccagcacacc
     1321 actaaaagta ttaatctcgc aagcaacatg ggcggaatta taatctgtgt aatctgtgta
     1381 tactattgcg gccaatgatt actgagaaaa cagaagcgcg accgctcgag ggcgctgtcg
     1441 tagccgcgcg gatcaaacag gaggtggcgg ccgaagtgga gaagttggtc cagacgctcg
     1501 gagtccgacc gtgtctggcg gctgtgcgcg tcggtgacga tccggcgtcg gccgtttacg
     1561 tacgcaacaa gatcaaggcg tgtgaagagg tcgggattgg atcggagcat cattcgttac
     1621 cggaatcgat cagcaccgcc gacttgctga atctcgttaa ctccttgaat gctcgtgacg
     1681 atgttgacgg catactgacg cagcttcctt tgccgcgtgg aatcgatgag acggcgatca
     1741 tcgaggctat cgatccgcgg aaagacgtag acggatttca tcccgtcagc gccggcaagt
     1801 tggcgctggg ccgcccagtt tttgtaccgt gtactcccgc cggcatcatc gagttgcttg
     1861 actacaacgg cgtcgaaatc cgcggtgcga atgcgtgcgt agtgggtcgc agtcagattg
     1921 tcggccggcc aatggcgcaa ctacttttgc agcgcgatgc cacggtcacg atctgtcact
     1981 cgcgcacgcg tgacctgccg gcagtgacac gccaggccga tctgctcgtc gttgccatcg
     2041 gtcgtgctgg aatgatcgga cgcgggcaca tcaagccggg cgcagttgtg atcgacgtcg
     2101 gcatgaatag agtttcagac gcgaacgagg tacgaagact gtttggtgaa gcagcggaga
     2161 agagattgga gatcgtcgca aagcgaggtt cgacgttggt tggcgacgtc aatcccgctg
     2221 aggcttcgga agtggccggc atgctgacgc cagtcccggg tggcgttggt ctactcacgg
     2281 ttgcgatgtt gatgaaaaac actttgaaag ccgcgaagtt gcgccgggct caggcgcaaa
     2341 atccgtaagc aattcgatgc tgcgtgtggg cctgacagga tcgattgccg tcggtaaatc
     2401 ctatgtcgct tcggtctttg cagaactagg ctgtcacgta ctcgatgcgg atcaaacagc
     2461 gcgtgaggtc gtgcttcccg gttctgcggg tcttgccgcg gttgcggcgg cattcgggag
     2521 tgacgtactc aactcggacg gggcgttaaa tcgccagcgg ctgggcgaga ttgttttcgc
     2581 gcacgaagac aaacggcagc aattgaacca gattcttcac ccgttcatca tcgcgcgcca
     2641 ggatgagatc atgcgcgagt gggaacgcga agatcctgac ggcatcggtg tcatcgacgc
     2701 ggcgttgatg atcgagtccg gcggctaccg ccggttcgat aagttgatcg tggtccactg
     2761 ccggccagaa gttcagcttg aacgtctgat gttgcgaaac aatctctcgc gcgaggaagc
     2821 gcaggcgcgg atcgattcgc agatgccgca ggcagagaaa cagaagtatg cggattttct
     2881 gatcgatact tccgacgggt ttgaaccaac cagaaagcga acgaacgaag tttacaacga
     2941 gctggtgaag ctaactggca agcgataaat gaagacgaag actgtgacaa gtttccgccc
     3001 ccgacggcta agacagttgc gttggacgca agtttacgca ctctcgctgg ttttctgtct
     3061 cgtgttgacc ggttgtttca atgaagagcc ttcgacctac tacggcaaga tcgtgccccc
     3121 gcgcgcgcag gagtttcgct ggagcgatgg cggactgccg cagacattcg atccggcgtt
     3181 tgctgctgcg ccgcccgata cggacgccgt tagagcactc ttcgagggat tgacggacta
     3241 cgatccgcaa acactggcgc cggttccagg cgtggcgcag cgctgggagt cgtcgcagga
     3301 cggccgcgtc tggacctttt acctgcgcga taatgcacgc tggtccaacg gcgagagtgt
     3361 cactgccggc gacttcgtgc gttcctggca acgcacgctg aagattggtc cactcgcgcc
     3421 acacacagag ttgctcaata acatcgaagg cgcaaccgca acgccgccgc cggtcaacgc
     3481 gacagaggcg gcgcctcact tcggcgccaa ggcgatcggc gaacacgttc tgcaagtcac
     3541 gctacaacag gcagacgaca gttttcctgc tctggtggcc catccggtgt ttcgtccggt
     3601 aaagctcgcc gatgcagaaa acacaaaacc catcgagtcg catgagttga tttcaaacgg
     3661 cgcgttcctg ctttcagccg cagaagaaga tcgcgtccgg ctcgaacgtg ctcgcactta
     3721 ttgggacggc gagtcagtct cactcgaacg cgtcacgttt gtaaacgctg cgaatcccga
     3781 agacgctctt tcggcttatc accagggcga gatcgacgcc gtcactaacg ctgcgttcga
     3841 gccgctcgcg ctcaagctgt tagctccgta caaagacttc cgccgctcca ccttcggcgc
     3901 tttgacctac tacgttttca atgtcgatcg cgagccgttc gacgacgttc gcgtgcgcga
     3961 agcgctcgcg ctggcgatcg atcgcgagcg tgtcagccga gacgatctcc gtggtgcgac
     4021 cgaaccggcg ggaaggttcc tgcctgacgc tatgtcggga gagaagccgg tcgtcgaaaa
     4081 gacggagttg ctcgatcacg acgtgaacaa ggcgcgcgag ctcctggccg aagccgggta
     4141 tcccgacggc gacgggttcc ccgtgatgag gctgctaatc aatcgcaacg aacaacagcg
     4201 aaccgtcgcg cagtcgatcg cggcgatgtg gcgtgcgacg ctcaacattc agaccgagat
     4261 cgtcatcaag agttgggacg aatacgaagc tgcgatccaa gcaggcgatt acgacgtcgt
     4321 gcgtcgcggt ctggtaatgc aaactaccag cgagctcaca aacatcgcga tgttgtttgg
     4381 ccgagattca cacccagcgc cggtacaacc gccggcaggc ctggcgaacc cgccggcgcc
     4441 ggaaccgcaa cccgctttga tcgagactga agcgcaggct ttgaaggaat tgaaggcgat
     4501 gccgatctat ttcgcgtctt cctactcgtt agtgaaacca tacgtttccg gattcgatcg
     4561 gaacgtgctg gacgcgccgt cgttgaaacg aaccaggctc gatacgaact ggagcgagca
     4621 agggcggtga aggcttcgga cgtgatcagt agcggcggcg tgatcgcgtt tcgcacggac
     4681 acgttctacg gactcggcgc cgatcctttc aacgcctcgg ctgtgcggcg catcagggaa
     4741 ctaaaaggcc gtgaggacga caacccgata ctgcttctga tctccgatcg tcctcaagtc
     4801 gagcgattgc tggtcgagtg ttcacacgaa tttaacatcg ctgcgcaaga gttatggccg
     4861 ggcccgctaa cgatagtcgg caaagccgcc gcgacattgc ccgaagaaat cacggcggga
     4921 acggggacag ttggtgttag actgcccgcc gacaaaagtg ttcgtgactt tgtgcgcgag
     4981 tgtggaggcg tcctgactgc aaccagcgca aacccttcgg gtcgcgaacc ggcccgttcg
     5041 gcacaggaag ccgcggcata ctttccgacc ggtctcgatt tgatcgtcga aggtggtgaa
     5101 gtgaacgcga cgaaaccttc gacagtttta gacgtatctt cttttccacc gcgcgtggtt
     5161 cgcgaaggcg cgatcgcgcg caacgtcatc gagcaactct ttgcttaagt atggtagggt
     5221 cctaacctga acgccgaaag gcgttcccga attccagccc acgggttgga gttcgcgaca
     5281 accctgggta ttgattcgat gtgtttacct cttatgctga aagcgttggc cag
//
LOCUS       DASTEY010000028         7090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1450857, whole genome shotgun sequence.
ACCESSION   DASTEY010000028 DASTEY010000000
VERSION     DASTEY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450857"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="pstS"
                     /locus_tag="VFO72_00490"
     CDS             <1..782
                     /gene="pstS"
                     /locus_tag="VFO72_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET9477788.1"
                     /translation="KSAPGEILHIPTVLGAVVITYNLSGVNQPLRFSPEALADIFLGK
                     ITKWNDSRIAADNPGVTLPANDITVVHRSDGSGTSAVFTDYLSKVSAEWKEKVGSGTS
                     PRWPIGLGGKGNEGVTGQVKNTPNTIGYVELAYAVQNRLPVALVKNGSGNFIEPTIDS
                     VSAAAAASAANTPDDLRVSITDAAGPQAYPISSYTYILVYKDQKDATKGKALVDFLWW
                     GIHEGESFAKELHYAPLPADIVTRAEGKIKLITAGGTSLRS"
     gene            950..1948
                     /gene="pstC"
                     /locus_tag="VFO72_00495"
     CDS             950..1948
                     /gene="pstC"
                     /locus_tag="VFO72_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9477789.1"
                     /translation="MSSASESDKLDFVGQHSTWRRVLSPTGNTGDAVFRVLMLGVALL
                     MVGIVIAMVVILTTDSMLSIRQFGFGFLTSQRWDPIKGDFGALPFIYGTIASSLIALL
                     ISVPFSLGIAIFLVEQAPDYISRPVGFLVELLAAIPSVVYGLWGIFVLAPFLRTYIQP
                     ALASVFGWSPFFKGQMTGIGLLTGGIILAIMVTPIISAVVRDVLAAVPGSQREAALAL
                     GATKWEMIRVVLVNGAPGIAGAVILGLGRALGETMAVTMVIGNRAQISASLFDPAYTI
                     ASAIANEFTEATGDLYLSSLVELGLILFMVTFVVNGIARVLVWNVTRKTGGVGRAA"
     gene            1948..2781
                     /gene="pstA"
                     /locus_tag="VFO72_00500"
     CDS             1948..2781
                     /gene="pstA"
                     /locus_tag="VFO72_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9477790.1"
                     /translation="MNNADQIRRANSAFMTGLTALCTLLAVGALLIILSHIAWEGIGS
                     LNVRFLTDVPRPVGEGGGIGNAIAGSLVLLLLASVIGLPLGICVGVYLAEIGSGRFGG
                     AVRFLVDTLTGIPSIVTGVFVYAILVLPMKHFSAFAGGVALALIMIPIVARTTEEMLK
                     LVPHSLREGALALGAPQWRVTLGVVIPAAAPGIATGAMLAIARISGETAPLLFTAFGS
                     RFFPTTLNDPIASLTVQIYNYAISPYDEWHAQAWAATLVLMTLVLGINIIVRFLTRKR
                     F"
     gene            2786..3616
                     /gene="pstB"
                     /locus_tag="VFO72_00505"
     CDS             2786..3616
                     /gene="pstB"
                     /locus_tag="VFO72_00505"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET9477791.1"
                     /translation="MPVQTEPPRIQIRDQRPAYIAGDQGKAKVSVRDLNFFYGRVQAL
                     HNISLDIPERIVMAFIGPSGCGKSTFLRTLNRMNDVIPGTRVAGQVLIDDHDIYAAGT
                     DVVELRRRVGMVFQKSNPFPKSIFDNVAYGLRINRLTSSGSELEGRVEQALKDAALWN
                     EVKDRLRTSALSLSGGQQQRLCIARALAIRPEVLLMDEPASALDPIATSKIEELIFDL
                     KKQYTIVIVTHNMQQAARVSDTTAFFLLGKLIEVNSTEKMFTAPNEKLTEDYITGRFG
                     "
     gene            3627..4286
                     /gene="phoU"
                     /locus_tag="VFO72_00510"
     CDS             3627..4286
                     /gene="phoU"
                     /locus_tag="VFO72_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9477792.1"
                     /translation="MDFHRHLDDELSSLRDRVLLLGGEAEQALERAMYSLTERDSEVA
                     RQVLDHDDEVDQLEVEIDRQCIDIIALRQPAARDLRFVISVAKMAPVLERIADHACNI
                     ARAAIDLNNEPELKSVLDLNRMAEHALEMLRAALDAFTSSDAAMAREVIKSDTEINDL
                     YNHTFHRLIELMVTEPATATRDARLLFVAKHLERIGDYVTDICELTVYMAEAAFIKHT
                     S"
     gene            4289..4975
                     /locus_tag="VFO72_00515"
     CDS             4289..4975
                     /locus_tag="VFO72_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9477793.1"
                     /translation="MARPILIIEDDADISESLKYNFEREGLPAVTAPTGEAGLSAALS
                     ERDPPRLIILDLMLPGMSGTELCRRLRREPATRRTPIIMLTAKTSESDRVAGLDLGAD
                     DYITKPFSVRELLARVRAVMRRADETMGKTYEDDRLTIDFDDIRVVCDGAKIKLTNKE
                     FTLLSVLARSADRVVTRQQLLDSVWGYSYYGDARTLDVHIRRLRQKLDKCGDCIETVV
                     GVGYRFVGCR"
     gene            4982..6127
                     /locus_tag="VFO72_00520"
     CDS             4982..6127
                     /locus_tag="VFO72_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9477794.1"
                     /translation="MIRRWLIAALTKREKVQSRESEDTDKPDDVFAPASLFEATIGGM
                     REGLLVVNRDMRVVAANPAAHKLFSASVAKLKSQRLTELTRNPAIYGAFLDGLKGREV
                     SGVKVETHGPERQVFDLRVVPIGNANGNSAQGALGVFFDITRTERLELVRQEFLSNVS
                     HELRTPLTAIMAFVETLESGAKEDPESCQRFLSIIRKNASRMHDLIDDILELTAIEGG
                     NIQLRAAPVELHALVNDVCASLAAQASARDITLRNNVAPEAVVNADARRLEQMLTNLI
                     DNGIKFGREHGSVTISHVSGPRDQIRVADDGDGIPAQHLERLFERFYRIDRARSREMG
                     GTGLGLAIVKHLALLHGGEVTVNSELGKGSTFTIHLPGNPAIPADKN"
     gene            6267..6461
                     /locus_tag="VFO72_00525"
     CDS             6267..6461
                     /locus_tag="VFO72_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477795.1"
                     /translation="MRIKRVARSASTFLTQLGAALVKTVVTSIVLGFVVVAVMQYMGV
                     PVPTAHELMGGVKRLAEILS"
     gene            6472..>7090
                     /locus_tag="VFO72_00530"
     CDS             6472..>7090
                     /locus_tag="VFO72_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9477796.1"
                     /translation="MSTELGWAYDVLGVKPGVSDSELKAAHRDLAKVWHPDRFLHDPR
                     LQDKAQEKLKEINEAYEQLISRHKRRVPPPPRSSPSSQPPPPSSRYRTPATPAEVVVP
                     TRRGIGWHWYVAASLVVFAVVFAVTTQKLVRQRQQVIETTEVEESIAQPTETEEPTHT
                     VRNRTENNSAPAATEAVTENAAPANETVLAVQTVTVVIDPASGLIA"
BASE COUNT         1514 a         1954 c         2081 g         1541 t
ORIGIN      
        1 tcaagtcagc tcccggcgag atactgcaca ttccaacagt cctcggcgcg gttgtgatca
       61 cttacaacct gagtggcgtg aatcagccgc tacgcttttc gccggaagca ctcgccgata
      121 ttttcctggg caagatcacg aagtggaacg attcgagaat tgccgctgat aacccgggag
      181 ttactttacc cgcgaacgac atcacggtgg tccatcgttc cgacggcagc ggcacgtcag
      241 ccgtgttcac cgactatctt tcaaaggtca gcgccgagtg gaaggagaaa gtcggatcgg
      301 gaacatcgcc gcgttggccc atcgggttag gcggcaaggg aaatgagggt gtaacgggtc
      361 aggtcaagaa cacgcccaat acgatcggtt atgtggaact ggcgtatgcg gtgcagaaca
      421 ggctgccggt ggcgctcgtc aagaacggta gtggcaactt cattgaaccg acgatcgatt
      481 cggtctctgc cgcggccgcc gcatctgctg caaacacgcc cgacgatctg cgtgtgtcga
      541 taacagacgc tgcgggaccg caggcgtatc caatctcgag ttacacctac atcctcgttt
      601 acaaagacca gaaagacgcg accaaaggca aggcgctggt cgactttctc tggtggggaa
      661 tacacgaagg cgagagtttt gcgaaggagt tacattacgc gccgctgccc gcggacatcg
      721 tgacgcgtgc ggaagggaaa attaagctga tcaccgcggg aggcacatcc ctccggtcgt
      781 gaaaacgctg aatttaaaga acacctcttt cccagggacc gcaggcgtct cgcctgctta
      841 gacgcgaatc gggcgctccc gtgagtgggc aggcgagacg cctgcggtcc cagggttgag
      901 acgtccaatt ggtttcagcc cgctttattg cgagagagtt gttgacgaca tgagctcagc
      961 gtcggaaagc gacaaactgg atttcgtcgg acagcactca acgtggcgtc gtgtgctttc
     1021 gccgaccgga aacacgggcg atgcggtctt ccgtgtgctg atgttgggcg tcgcgctgct
     1081 gatggttggc attgtgatcg cgatggtcgt gatcctgacg accgactcca tgctttccat
     1141 tcggcagttt ggtttcgggt tcttgaccag ccagcgttgg gatccgatca agggtgactt
     1201 tggtgcgctg ccgttcattt acggcacgat tgcttcatcg ctgatcgcgc tgctgatcag
     1261 tgttccgttt tcactgggta ttgcgatctt cctggtagag caggcgccgg actacatctc
     1321 gcgtccggtt ggttttctcg tcgaactgct cgcggcgatt ccctcggtgg tctatggcct
     1381 ctggggcatc ttcgtgctgg ctccgttcct gagaacctac atccagccgg cgctcgcgag
     1441 cgtgtttggt tggtcgccat tcttcaaagg acagatgact ggcattggct tgctcacagg
     1501 aggaatcatt ctcgccatca tggtgacgcc gatcatctcg gcggtagtcc gcgatgtgct
     1561 ggcggcggtg ccgggatcgc aacgcgaagc ggcactcgcg ctcggtgcca cgaagtggga
     1621 gatgattagg gtcgtgttgg ttaacggcgc tccaggcatc gccggcgcgg tgattctcgg
     1681 tctcggtcgc gcactcggcg agacgatggc cgtgacgatg gtgatcggca accgggcgca
     1741 gatcagtgcg tcgcttttcg atccggctta cacgatcgct tcggcgattg cgaacgagtt
     1801 cacggaagca accggcgatc tctatttgag ttcgctcgtc gaacttgggc tgattctgtt
     1861 tatggtgacg ttcgtcgtaa acggtatcgc tcgcgtgctc gtttggaacg tgactagaaa
     1921 gactggtgga gtcggaaggg ctgcgtaatg aacaacgccg atcaaatccg ccgtgcaaac
     1981 agtgccttca tgacgggcct gaccgcgttg tgcactctgc tcgctgttgg cgcgctgctg
     2041 atcattcttt cgcacattgc gtgggaagga atcggttctc ttaacgttcg cttcctgacc
     2101 gacgtcccgc ggccggtcgg cgaaggtggc ggcatcggaa acgcgatcgc cggatcgttg
     2161 gtgttgctcc tgctcgcgtc ggtcattggt ctgccactcg gaatttgcgt cggcgtttat
     2221 ctcgccgaaa ttggcagtgg gcggttcggc ggcgcggttc gttttcttgt cgacacgttg
     2281 acgggcattc cttcgatcgt aactggtgtt tttgtttacg cgattctggt tctgccgatg
     2341 aagcacttct cggctttcgc aggtggtgtc gcattggcac taatcatgat tccaatcgtc
     2401 gcacggacca cggaagagat gttgaaactg gtgccgcaca gtctacgtga aggtgccctg
     2461 gcgcttggcg cgccgcagtg gcgcgtgacg ttaggcgttg tgatcccagc ggcagcgccc
     2521 ggaatcgcca ccggagcaat gctggctatc gcgcgcattt cgggtgaaac tgcgccactg
     2581 cttttcacgg ccttcggcag ccgatttttc ccgacgacac tgaacgaccc gatcgcgtcg
     2641 ttgaccgtac agatctacaa ctacgcaatc tcgccgtacg acgaatggca cgcacaggca
     2701 tgggccgcaa cgttggtgct gatgacgctg gtcttgggaa tcaacatcat cgtgcggttt
     2761 ctgacgagga agaggttcta ggtctatgcc cgttcaaacg gaaccgccaa gaatccaaat
     2821 ccgggaccag cgtccggcgt acatagccgg cgatcagggt aaagcaaagg tctctgttcg
     2881 cgatctgaac ttcttctacg ggcgcgtgca ggcgctgcac aacatctcac tcgacattcc
     2941 cgagcgcatt gtgatggcgt tcattggtcc atcgggctgt ggcaagtcga cattcctgcg
     3001 cacgctgaat cgaatgaacg acgtgattcc cggaacgcgt gtcgcgggcc aggtattgat
     3061 tgacgatcat gatatctacg cggctggaac cgacgtcgtg gagttacggc gccgcgtggg
     3121 aatggtgttt cagaaatcga atccatttcc gaagtcaatt ttcgataacg tggcctatgg
     3181 tctgcgtatc aatcgcctga caagcagcgg cagcgagctg gagggccgcg tcgaacaggc
     3241 actcaaagac gccgcactgt ggaacgaagt taaagatcgg ctgcggacgt cagcgctgag
     3301 tctctcaggc ggacaacagc aacgtttgtg tatcgcgagg gcgttggcga ttcgcccgga
     3361 agtgttgttg atggacgagc ccgcttcggc actcgatccg attgccacgt caaaaattga
     3421 agagctgatc ttcgatctga agaagcagta cacgatcgtg atcgtcacgc acaacatgca
     3481 gcaggcggcg cgagtttccg acaccacggc gttcttcctg ctgggtaagt tgatcgaagt
     3541 gaattctact gagaagatgt ttactgcccc caatgaaaag ttgaccgagg attacattac
     3601 cggaaggttt ggttaagaag taacttatgg attttcatcg tcaccttgac gacgaactga
     3661 gttcattgcg cgaccgcgtg ctgctgcttg gcggcgaagc ggaacaggca ctcgagcgcg
     3721 cgatgtattc actcaccgaa cgcgacagcg aagtagcccg ccaggttctc gatcacgacg
     3781 acgaggtcga tcaactggag gtcgaaatcg atcgccagtg catcgacatc atcgccttgc
     3841 ggcaaccggc ggctcgcgat cttcgcttcg tgatctcagt cgcaaaaatg gctccggtgc
     3901 tcgaacgcat cgccgaccac gcctgcaaca tcgctcgcgc ggctatcgat ctgaacaacg
     3961 aaccggagct caagtcagtg ctcgatctga accgtatggc ggagcacgcg ctggagatgt
     4021 tgcgcgctgc actcgatgct tttacttcaa gcgacgccgc gatggctcgc gaagtcatca
     4081 agagcgacac tgagattaat gacctttaca accacacgtt tcatcggctc attgaactga
     4141 tggtcaccga gccggcgacc gcgactcgcg atgcgcggct tcttttcgtg gcaaagcacc
     4201 tggagcgcat aggcgactac gttacggaca tttgcgaact caccgtctat atggccgaag
     4261 cggcattcat caaacacacc agctaatcat ggcccgccca atcctaatca tcgaggacga
     4321 tgccgacatc tcggagagtt tgaagtacaa cttcgaacgc gaaggtcttc cggccgtgac
     4381 ggcaccaaca ggcgaagcag gtctgtccgc ggctttgagc gagcgcgatc cgccccgact
     4441 gataattctc gacttgatgt tgccgggcat gagcggcacc gagctgtgcc ggcgactgcg
     4501 gcgtgaaccc gcaacacgtc gcacgccgat catcatgttg actgcgaaga cttccgaatc
     4561 cgatcgcgtt gccggactcg atctcggtgc ggacgattac atcaccaaac cgttttcagt
     4621 acgcgaactg ctcgcgcgag tgcgcgccgt catgcgtcgc gccgatgaga cgatgggcaa
     4681 aacgtatgaa gacgatcgtt tgaccatcga cttcgacgac atccgcgtag tttgcgatgg
     4741 cgcgaagata aaattgacga acaaagagtt caccctgctc tctgtgctcg cgcggagcgc
     4801 tgaccgcgtt gtgactcggc aacagttgct cgatagtgtt tggggttaca gttactatgg
     4861 cgatgcgcgc acgctggacg tgcacatacg ccgtttgcga cagaagctcg acaaatgtgg
     4921 cgactgcatc gaaacggtgg tgggagttgg ctatcggttc gttggttgca ggtgatttgt
     4981 tgtgatcagg cgctggctaa ttgcagcact tacaaaacgc gaaaaagtgc agtcgcgcga
     5041 gtcagaggac actgataaac cggatgacgt cttcgccccc gcctcactct ttgaagcaac
     5101 cataggcggg atgcgcgaag ggctattggt agtgaacagg gacatgcggg tggtggccgc
     5161 caatcccgcg gcgcacaaac tattcagcgc ttcagtcgcc aaactcaagt cacagcgttt
     5221 gactgagttg acccgtaacc ccgcgattta tggtgcgttc ctcgatggtc tcaaggggcg
     5281 cgaagtcagt ggcgtgaagg ttgagacaca cggaccggaa cggcaggttt ttgaccttcg
     5341 cgttgtaccg atcggcaatg caaatggaaa cagcgctcaa ggcgcactcg gcgttttctt
     5401 cgacatcacc cgcaccgaac gtctggagct cgtccgtcag gaatttctct ccaacgtctc
     5461 tcacgaacta cgcacgccgc tcaccgcgat catggctttt gtcgagactc tcgaaagtgg
     5521 tgcgaaggaa gatccggaaa gctgccagcg ctttctttcc atcattcgca agaacgcttc
     5581 acgaatgcac gatctcatcg acgacattct cgagctgacg gcgatcgaag gcggcaacat
     5641 ccaactgcgc gccgcgccgg ttgaactgca tgcgttagtt aacgacgtct gcgcttcttt
     5701 ggcagctcaa gcgagtgctc gcgacattac tctcagaaac aacgtcgcgc ccgaagcagt
     5761 ggtcaacgca gatgcgcggc ggctcgaaca gatgctgaca aacctcatcg acaatggcat
     5821 caagtttgga cgcgagcacg gtagtgtcac gatcagtcac gtatcggggc cgcgagatca
     5881 aatccgcgtg gccgacgatg gtgatggcat tccggcccag catctcgagc ggctgttcga
     5941 gcgtttttac cgcattgacc gtgctcggtc gcgggagatg ggtggcactg gattgggtct
     6001 ggccattgtc aagcaccttg ccctgttaca cggcggagag gtgacagtca actccgagct
     6061 cgggaaaggc agtacgttta ccatccacct gcccggaaac cctgctattc cagcagataa
     6121 aaactgaaaa acaatgttgt aaagacgcct gagctttgcc ataatagtcg ccgagttttg
     6181 ccctcgttct cttccccgac ggccccagga aggtcctcaa aaaagctagt ttcgaccgaa
     6241 cgaaaagcgg tctggggaga tcaactatgc gaattaaacg agttgctaga tccgcgtcga
     6301 cgtttttaac tcagctcggt gccgcgttgg tgaaaactgt ggtcaccagc attgttctgg
     6361 gatttgtggt ggtcgcagtg atgcagtaca tgggcgtgcc cgtacccacc gcacacgaac
     6421 tgatgggcgg agttaaacgg ctcgcggaga tcctttcgta agaggaaatc attgagcaca
     6481 gaacttggct gggcctacga cgtgctggga gtaaaacccg gcgtttcaga tagcgaattg
     6541 aaagcagcac atcgggacct ggccaaagtc tggcatcccg atcggttcct gcacgacccg
     6601 cgtttacagg acaaggcgca ggagaagttg aaagagatca atgaggctta cgagcagttg
     6661 atctcacgtc acaaacgccg cgttccgcca ccgccacgct cttcaccctc gtcacagcca
     6721 ccaccaccat catcccgata tcgcacgccg gcaactcccg cagaagtcgt tgtcccgaca
     6781 cgccgcggga ttggctggca ttggtatgtt gcggcttcgc tggtcgtatt tgctgtcgtg
     6841 ttcgctgtca cgacgcaaaa actcgtgcgc cagcgtcagc aggtaatcga aacaactgag
     6901 gttgaggaat cgatcgccca acccacggag actgaggaac cgacgcacac ggttcgtaat
     6961 cgaactgaga acaattcagc gccggccgcc acggaagctg tgactgaaaa cgctgctcct
     7021 gcgaacgaaa cggttctcgc ggtgcagacg gttaccgtcg taatcgatcc cgcgagtggt
     7081 ttaatcgcga
//
LOCUS       DASTEY010000029         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_115856, whole genome shotgun sequence.
ACCESSION   DASTEY010000029 DASTEY010000000
VERSION     DASTEY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115856"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFO72_00535"
     CDS             complement(<1..850)
                     /locus_tag="VFO72_00535"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477797.1"
                     /translation="MSSHPRLPKILILSSDTGGGHRSAAAAIVAGVQKFFEGESYAVR
                     VVRAVEESHHLSAKLVRLYNWILRNRQHWMKYFYWCMNRVRPETREFFHKRCVGYVLD
                     LFERWCPHVVVSVHPLTQHIFGRVMKELNLADRVPLVSVVTDPCYGFWKGWACDAVSL
                     YLVASEEARRQLIDYGVAPERIKISGMPVHPKFAYPGEEAAQAARRALGLDPEKFTVF
                     VNAGWIGGGNIPQIFRELVRGELDVQAIFLAGKNEDLRAAAESIALDAPFPIKVIGYS
                     DEIEKLM"
     gene            complement(825..1982)
                     /locus_tag="VFO72_00540"
     CDS             complement(825..1982)
                     /locus_tag="VFO72_00540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9477798.1"
                     /translation="MDFTTKELLKRYAKLAIGQPFSPVLLNILVTSVCDMRCTHCFFT
                     DELDDRPRKKLQMKTHEIERISETLGGNLGVLILAGGEPFTRKDLPEIARAFYTNNKL
                     ESIYIMSNGQIQKRIFPDVTRVLQECPNLNVTVALGIDGPKAEHEKIRQKTGSWDIAI
                     DTARQLQEIKKEYPRLDVQTCTCFMNSNQDTIFEWYDFLKYDLKPDKVNFNYIRPPSA
                     DPKELEIDHSRYARLAQMIDDDSRHAAIKNNYGGKAGFFKAAIDIYMHGLIAKTQETQ
                     TAQMKCYAGTAGGVVYDEGTVSSCENLAPIGNLRDFDWNFQKLWLSPAMKERRKQAAS
                     GCFCTHESNSYYPSLPFNPGHLIQIKKLEREMKKAQSKTQQGAYVFAPSLT"
BASE COUNT          429 a          572 c          550 g          485 t
ORIGIN      
        1 tcatcagttt ttcaatttcg tccgaatagc cgatcacctt gatcgggaac ggcgcgtcga
       61 gagcaattga ttcagcggct gcgcgcaaat cttcgttctt gccggccagg aagattgcct
      121 gcacgtcgag ttcaccacga actagttccc ggaagatctg cggtatgttg ccaccgccga
      181 tccaaccggc gtttacaaac accgtgaact tttccggatc caaacccagc gcgcgccgcg
      241 ccgcttgagc tgcttcttcg cccggataag caaacttcgg atgcaccggc ataccggaaa
      301 tcttgatgcg ctcgggagca accccgtagt cgattaactg ccggcgcgct tcttcgctcg
      361 cgaccaggta aagcgaaact gcatcgcagg cccagccttt ccaaaaaccg taacaaggat
      421 cagtcacaac cgacacgagc ggaacacgat ccgcgaggtt cagttccttc ataacgcgac
      481 cgaaaatatg ttgcgtgagc ggatgaacgc tgaccacgac gtgtggacac cagcgctcaa
      541 acagatcgag cacataacca acgcatcgct tgtgaaaaaa ctcgcgtgtc tcgggcctga
      601 cgcggttcat gcaccagtag aagtatttca tccagtgctg gcggttgcgc agtatccaat
      661 tgtacaaacg cacgagcttg gcgctgagat gatgcgactc ctcgacggca cgaacgaccc
      721 tgaccgcgta cgactcacct tcgaaaaact tctgcacgcc ggcgacgatc gccgcggcgg
      781 cggagcgatg cccaccaccc gtgtcggacg agaggattaa gatcttaggt aagcgagggt
      841 gcgaagacat aggctccctg ttgagtcttg gactgcgctt tcttcatttc gcgctccagt
      901 ttcttgatct ggatcaagtg ccccggattg aacggcagcg acgggtaata actgtttgac
      961 tcgtgcgtgc agaaacaacc gctcgccgct tgcttgcgac gttctttcat tgccggcgac
     1021 aaccagagct tttgaaagtt ccagtcaaag tcgcgcagat tgccgatcgg cgccaggttt
     1081 tcgcatgacg aaaccgtgcc ttcgtcgtaa acgacacccc cggcagtgcc tgcgtagcat
     1141 ttcatctgcg cggtctgcgt ctcctgcgtc ttcgcgatca ggccatgcat gtagatgtcg
     1201 atcgcggcct tgaagaagcc ggctttgccg ccgtagttgt tcttgattgc ggcgtgccgt
     1261 gagtcatcgt cgatcatttg cgcgagtcgt gcgtagcgcg aatgatcgat ctcgagctcc
     1321 ttcggatccg ctgaaggcgg gcgaatgtag ttgaagttta ctttgtccgg cttgaggtcg
     1381 tatttcagaa agtcgtacca ctcgaagatc gtgtcctggt tcgagttcat gaagcaggtg
     1441 cacgtttgta cgtcaagccg cgggtattcc ttcttgatct cctgaagctg ccgggcggtg
     1501 tcgatggcga tgtcccacga gcccgttttc tggcggatct tctcgtgctc ggcttttgga
     1561 ccgtcgattc cgagcgccac cgtcacgtta agattgggac actcctgcaa cacccgcgtc
     1621 acgtccggaa agattctttt ctgaatctgg ccgttggaca tgatgtagat cgattcgagc
     1681 ttattgttgg tgtagaacgc acgtgcgatt tcggggagat ctttgcgtgt aaacggttcg
     1741 ccgccggcca ggatcaaaac accgagattg ccgccgagtg tttcggagat gcgctcgatc
     1801 tcgtgcgtct tcatttgcag ctttttgcgt ggacgatcgt cgagttcgtc tgtgaagaag
     1861 cagtgcgtgc agcgcatgtc gcacacggac gtgaccagta tgtttagcaa caccggcgaa
     1921 aatggttgtc caatcgctag ttttgcgtac cgtttcaaca gttctttcgt cgtaaaatcc
     1981 atttcccctc aatctctttc tcgcagcaca acgcgcacga gaacttacac tactaa
//
LOCUS       DASTEY010000030         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_429022, whole genome shotgun sequence.
ACCESSION   DASTEY010000030 DASTEY010000000
VERSION     DASTEY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429022"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFO72_00545"
     CDS             complement(<1..372)
                     /locus_tag="VFO72_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477799.1"
                     /translation="MKKLAVFLVVLCSAWIANAQEPTADSGEKRVPLNEAAVAFDGTG
                     AAALEATLRTTPLNGAPDAPVTNVRMIVKNVSSMSYAFVSGLVTFYDAAGIRCGEGIF
                     KADALAVNESFETDTPGLRIRC"
     gene            complement(446..1003)
                     /locus_tag="VFO72_00550"
     CDS             complement(446..1003)
                     /locus_tag="VFO72_00550"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9477800.1"
                     /translation="MELKLKSCSLRGWEWRDLEAIVRHANNRNVWINLRDRFPHPYTV
                     NDARSWLDAVVCEKPLTSFAIAVADEAVGGIGFTVQHDVARRSAEIGYWLGEEFWGRG
                     IATEALIAVTDYAFSNYDVCRLYAHVFDWNIASARVLEKAGYVFEGRLRKSVTKDGQT
                     IDQLMYAIIREPGTAGVSPATHGSA"
     gene            complement(994..2271)
                     /gene="larA"
                     /locus_tag="VFO72_00555"
     CDS             complement(994..2271)
                     /gene="larA"
                     /locus_tag="VFO72_00555"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HET9477801.1"
                     /translation="MSTEIELPYGRSAVTFSWDGDRFSTISEVSNLEKPLTDFQIGAA
                     FDSPIGSPPLDEIVHTSDSVLIVVSDATRATASAQVVNLLVRRLVQAGVSPSRMAVIF
                     ATGIHRAVTEQEKLELLTPFIVQRLKVLQHDAYDPSKLNSFGQTESGIQIELSSTLRE
                     YSRVIVIGGVNFHYFAGFTGGRKSICPGLASAETIKRTHMLALDFTTGGRRKGVGTGL
                     LDGNSVHEQCERIAQVVAPAFSVNTIVNEQKRLVKLFCGDWRLACRAACEHYLDHNSV
                     GIPEKRDLVVASCGGFPYDINLIQAHKALDMASYACKDNGTIVLLAECGDGLGRPDFM
                     KWFESADSRALAERLRNEYEVNGQTAWALLTKAERYRIYLVSQLPDEQVRRMRMTPAR
                     SIAEALKDAGEKAGYVLPRGAAVLPRIEKTKWN"
     gene            complement(2268..2510)
                     /locus_tag="VFO72_00560"
     CDS             complement(2268..2510)
                     /locus_tag="VFO72_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477802.1"
                     /translation="MELSLLITLDLREQAALQAALVTHGAPDALVTLALTGACRIGSM
                     DEARQLRKWLAEARTAGSSDFAALHVIERAMIEFGL"
BASE COUNT          619 a          833 c          687 g          596 t
ORIGIN      
        1 gcaacgaatt ctcaatcccg gcgtgtctgt ttcaaacgat tcattcaccg ccagcgcatc
       61 cgctttgaat atcccttcgc cacaacgtat tcccgcggcg tcgtagaatg tgactaaccc
      121 ggaaacaaac gcgtacgaca tcgaactcac gtttttgacg atcatgcgaa cattcgtaac
      181 cggggcatcg ggcgcgccgt tcaaaggagt agttctcaaa gtagcttcca atgcggcggc
      241 gcctgtgccg tcaaaagcaa cggccgcttc gttcagcggt acgcgctttt caccggaatc
      301 ggccgttggt tcttgcgcgt tcgcaatcca ggcgctgcat aagacaacca ggaagacagc
      361 gagttttttc attgagttgg tctcctccgc gcttatcagt tctctttacc tgggaccgca
      421 ggcgtctcgc ctgcaaaatc tcagatcagg cgctgccgtg agtcgcaggc gagacacctg
      481 cggttccagg ttcgcgaata atcgcataca ttaactggtc gatcgtctgc ccgtccttgg
      541 tcacactctt tcgcaaacga ccctcgaaaa cataacccgc cttttccagc acgcgcgctg
      601 aagcgatgtt ccaatcaaac acgtgcgcgt acaagcggca gacatcgtaa ttggaaaagg
      661 catagtccgt cacggcaatt aaagcctcag tggcaattcc tctgccccaa aactcttcgc
      721 ccaaccagta accgatctcg gccgaacgtc gcgccacatc gtgctgcacg gtgaaaccaa
      781 tgccgccaac cgcttcatcc gcaacggcaa tcgcgaagct tgttaagggc ttctcacaaa
      841 cgacggcatc gagccagctg cgcgcatcat taacggtgta aggatgggga aaccgatcgc
      901 gcaggttgat ccagacgtta cggttgttcg cgtgacgcac tatcgcctcc aggtcgcgcc
      961 attcccaacc acgcaagctg cacgacttca acttcagttc cacttcgtct tctcgattcg
     1021 cggcaatacc gcggcgccac gcggcaacac gtaacccgct ttctcgcccg catccttgag
     1081 agcctccgcg attgatcgtg ccggcgtcat tcgcatccgc ctcacctgct cgtcgggcaa
     1141 ttgcgaaacc agatagattc gataacgttc ggcctttgtc agcaaagccc acgccgtctg
     1201 gccgttgact tcgtactcat ttcgcagccg ctcagccagc gcgcgtgaat ccgcggactc
     1261 gaaccatttc ataaaatccg gacgacccag tccgtcgcca cactccgcca acagaacaat
     1321 cgtgccgtta tccttgcagg cgtagctcgc catgtcgagg gctttgtgcg cctgaatcag
     1381 attgatgtcg taaggaaagc cgccgcaact tgcgacgacc agatcacgct tttccggaat
     1441 tccaacggaa ttgtggtcca ggtaatgttc acacgccgca cggcacgcaa gccgccaatc
     1501 gccgcagaac aacttcacta gccgtttttg ttcgttaacg atcgtgttga cgctgaaagc
     1561 aggagcaacg acctgcgcga tacgttcgca ttgttcgtgc acggaattgc cgtcgagcag
     1621 tccggtgccg acaccctttc tccggccgcc agtagtaaag tccagcgcca gcatgtgtgt
     1681 tcgcttgatc gtttctgctg acgccaggcc aggacagatt gacttgcgcc cgcctgtaaa
     1741 tccggcgaag tagtgaaagt tgacgccgcc gatgacgatc acgcgggagt actctcgcag
     1801 cgtactgctt aactcgattt gtatgccgct ctccgtctgc ccgaacgaat tgagcttcga
     1861 aggatcgtaa gcgtcgtgct ggaggacctt cagacgctgc acgatgaagg gtgtcagtag
     1921 ttcgagtttt tcctgctcag tgaccgcgcg atgaattccg gtcgcaaaga tcactgccat
     1981 tcgcgacggc gaaacaccag cctgaaccaa ccggcgcaca agcagattaa caacttgcgc
     2041 actggccgta gcgcgcgtcg catccgagac cacgatcaaa accgaatcgc tcgtgtgtac
     2101 gatttcatcc agcggaggtg aaccaattgg tgagtcaaac gcggcgccta tttgaaaatc
     2161 cgtcagtggc ttttctaaat tcgagacctc tgaaatggtt gaaaaccgat ccccatccca
     2221 actgaacgtc accgcactgc gtccgtaggg cagctcgatt tcggtgctca tagaccaaac
     2281 tcgatcatgg ctctttcaat gacgtggagg gcggcgaaat ccgaactgcc ggccgtgcgc
     2341 gcttccgcga gccacttacg caactgtcgc gcttcgtcca ttgacccgat gcgacaagcg
     2401 cccgtgagcg caagcgtaac caatgcgtcg ggcgcgccgt gagtcaccag tgcggcttgc
     2461 aatgccgcct gctcgcgcag atcgagcgta attagaagac tcagttccat ttagtgcttg
     2521 gttcttagtg ctttgtactt tgttttcaca gctaactttc gtccttacgc tgaacgcaca
     2581 atcgcgaaca aagtacaaag cactaagctc gaagttcaat tccgatccga acggaaacac
     2641 gctgtgcccg catgacaaaa gtgaaaggtc tcgtcttcaa aatccgacgt ttccatctga
     2701 atcgtcaggt ggtcgacgcc gaaccgatcg tgcag
//
LOCUS       DASTEY010000031         5868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1615611, whole genome shotgun sequence.
ACCESSION   DASTEY010000031 DASTEY010000000
VERSION     DASTEY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615611"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFO72_00565"
     CDS             complement(<1..484)
                     /locus_tag="VFO72_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9477803.1"
                     /translation="MPYIRVNETGFRPHDVKTAIAFGREPLPSDFRLLNTTTNAVAFT
                     GRAVPVSGGWGEFPYHAELNFSSVREDGVYVIESGSTRSPQFSISGNAHGEIADQLLE
                     FMRQQRCGYNPFVDAVCHSFDGRTAYGPLPAGTYINASGGWHDAGDQLKYLLTSSNAT
                     A"
     gene            794..1096
                     /locus_tag="VFO72_00570"
     CDS             794..1096
                     /locus_tag="VFO72_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9477804.1"
                     /translation="MMDIEHWRKEIDEVDAELLRLLNIRARLALKVGALKKSARLPFC
                     DPNREQYVLTRLQDINTGPLDDRAVYKLFRRIIRESRRVQTNVAPAKEFMRRRGIS"
     gene            1093..1671
                     /locus_tag="VFO72_00575"
     CDS             1093..1671
                     /locus_tag="VFO72_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET9477805.1"
                     /translation="MSSGVNKPTRVAFQGERGAFSEEAALKLLGDEIELVPLKTFDAL
                     FSSVDNGEADYVLAPVANTIAGPVRTSLNLLERSSLVMIAEVEIPIEQHLIGFPTATL
                     THIETVQSHPVALAQCRRFFADNPRLKAIDADDTAGSVAEVVRRGDVTCAAIAGRRAA
                     ELYGASIIRESIQDCPKNFTRFVLLAMKGNES"
     gene            1668..2684
                     /gene="aroF"
                     /locus_tag="VFO72_00580"
     CDS             1668..2684
                     /gene="aroF"
                     /locus_tag="VFO72_00580"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9477806.1"
                     /translation="MIVNMASTATPQEINHVIDRIRECGFQAHVIHGEERTVIGAVGN
                     SNRRSEIEALRAAPGVAEVIQIAHPFKLVSRQLRQARTVVDVRGIKIGGDELVVIAGP
                     CSVESQDQLMETAHAVKAAGADMLRGGAYKPRTSPYDFQGLGVEALRFLKHASRETGL
                     PIVTEVMSETDVEIVAEYADVLQVGARNMQNFSLLRKLAPVSKPILLKRGPSATVKEW
                     LLAAEYLLSGGNANVILCERGIKTFETATRNTLDLAAVALVKELSHLPVLADPSHGTG
                     MRSLITPMSKAAAAAGADGLLIEVHPCPERALSDGPQSLDLGGFANLMNELGKQPLRD
                     VKAA"
     gene            complement(2917..>5868)
                     /locus_tag="VFO72_00585"
     CDS             complement(2917..>5868)
                     /locus_tag="VFO72_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HET9477807.1"
                     /translation="KLDMDFVRVRKADGTVVNAAATDVQDLSAPVAREAPVYTDLRHK
                     HITVPGLRPGDVLEYHIVVTVHTALAQDQFWVEHDFIKKGMIVLSDELTVNIPAESKV
                     KLKTQPGYEPTTREQDGRRIYSWKHANLKPDSEEKPKEGEEEEEKEDAVEPEEVRPDV
                     QLTTFQSWDEVGQWYAALQRDRVVPDEKIKVKAEELIKGRTTEKEKAQALYEYVAKNF
                     RYVSLSLGQGRYQPHAATDVMVNQYGDCKDKHTLLASMLAATGLRAYPVLMNSFRKID
                     VDIPSPGQFDHVISAIPLGNETLWADTTAEIAPVGLLNPRLRNKKALLIPTTGPARLE
                     TTPAEPPFLSSEVVKVEGTVDELGKITAHAHLILHGDAEMFMRFIFRRTPKSNWKNLG
                     FYLGMAGGIEGDITDVKATDPADLAKPFEIEFNVTRNDFLDWSSKKVKLQLPFPPFSL
                     PPFTGRKPTSKKPLELGPPIDITYSLKLTLPAKYQARLPLPLKVTRDYGEYAASYKLE
                     GQTLIGERTLRLRQRELPAERLQDYQAFAAAARSDAAQTLQLETEAAGAPALSDTVKT
                     ADLLQAAQVAMSNQNFVVAEQLLKRVLEKDPKHKTVRRDLGPVLAAQRKLAEAVEVLR
                     EQTKINPFEEHTYDLLGSVYWQQQDYANAEQAFRKQLEVTPLDQAARSNLGQMLVDWR
                     KYKEAVPELEQAISLAPENEMLHVALGRAYLNLGEEEKGIKAFEEALKLDRSPLVLND
                     IAYFLATQKLQLDKALQYAESAVTSIASSLRNVEASNLTMDDLGDAASLGAYWDTLGW
                     VYFQKGDLDAAERYISAAWLIHQYSEVGHHMGAIAEKRGNKEEAIRMYAQGAVAYRVV
                     PEARESLLKLVSADAVEKLLQTAKSDLANYNKLDVGAMVANLKEPAEAEFYLVFAPDA
                     TRKAQVVEVKFIKGADSLKPLASQLKAVKYPLVFPDSAPTKIVRRGTLQCKPGACSLT
                     LLTPDQITSVD"
BASE COUNT         1223 a         1672 c         1658 g         1315 t
ORIGIN      
        1 gggcggtggc gttgcttgaa gttaacagat acttgagttg atctccggca tcgtgccaac
       61 ctccggacgc gttgatgtac gtcccggccg gtaatgggcc ataagcggtg cggccgtcga
      121 agctgtgaca aacagcatcg acgaatgggt tgtacccgca gcgctgctgg cgcatgaatt
      181 cgagtagttg atccgcgatc tccccgtggg cgtttccact gatcgaaaac tgtggtgacc
      241 gtgtggaacc tgactcgatt acataaaccc cgtcttcacg cacggaactg aagttcaact
      301 ctgcgtggta agggaattct ccccagccgc cggatactgg aactgcgcga ccagtaaagg
      361 cgaccgcatt ggtcgtggtg ttcagaaggc gaaagtcgct gggtagcggt tcgcgcccaa
      421 aggctattgc cgtcttcacg tcgtggggtc tgaagccggt ttcatttacg cggatgtaag
      481 gcacggattg ggcgtccgcg ctggggacca aaaacaagag aacggagaag gtcagaaccg
      541 taaatttcgc gaaagcgaca attttcttga caccgttttt cactcgcgcc tatattagcg
      601 tccgtcatgc gtagtactcg cacatttttc tacttcgcct ttactttccg ctccttcttt
      661 tacggggaga gcgggctggc gatgtgcgaa aaggatgtgc tgaagaccta aaaagcaagc
      721 acaacacctt caaacgagcc agccgcgatc ctgaaagtcg cggctggttt ttttgttgcc
      781 agacggagca gccatgatgg acatagaaca ctggcgtaag gagatcgacg aagtcgacgc
      841 cgaattgctg cgcctgctta acatcagggc gcggctcgcc ctgaaagtcg gcgcgctcaa
      901 gaaatccgct cgcctgccgt tttgcgatcc caaccgcgag caatacgtcc taactcgttt
      961 gcaggacatc aacacgggcc cgctcgacga ccgcgcagtc tacaaactct tccggcgcat
     1021 cattcgcgaa tcgcgaaggg tccagaccaa cgtcgcgcct gcaaaagaat tcatgaggag
     1081 acggggaatc tcatgagttc aggggtaaac aaaccaacac gcgtcgcatt tcagggcgaa
     1141 cgcggcgcgt tcagcgaaga ggctgcactg aagttgctcg gtgacgagat cgagctcgtg
     1201 ccgctaaaga ccttcgacgc gctcttcagc agcgttgaca acggcgaagc agattacgta
     1261 ctcgcgccgg ttgcgaacac aattgccggg cccgttcgga catctctcaa tctgttagaa
     1321 cgtagttcgc tggttatgat tgccgaagtg gagataccga tcgaacagca cctcatcggt
     1381 tttccgacag caaccttaac tcatatcgaa acggtgcaat ctcatcccgt ggcgctggcg
     1441 cagtgccgcc gctttttcgc agacaatccc cggctcaaag cgatcgacgc tgacgacact
     1501 gccggcagcg tcgccgaagt tgtccgccgc ggcgacgtaa cgtgcgcggc gattgccgga
     1561 cggcgcgcag ccgaactcta cggcgcgtcg atcattcgcg aatctattca agactgtccc
     1621 aagaacttta cccgctttgt tttactagca atgaaaggca atgaatcatg atcgtcaata
     1681 tggcaagtac cgccacccct caagagatta atcacgtcat cgatcgcata cgagagtgcg
     1741 ggtttcaagc gcacgtcatt cacggcgaag aacggaccgt tatcggcgct gtcggtaaca
     1801 gcaatcgccg ttcagagatt gaggccctgc gcgcggcgcc gggtgttgct gaagtgattc
     1861 agatcgcgca tccgttcaag ctcgtgagcc ggcagttgcg gcaggcgcga acggttgtcg
     1921 atgtccgagg catcaagata ggtggcgacg aactggtagt gatcgctggt ccatgctccg
     1981 tggagtcgca agatcaattg atggagacgg cgcacgcagt gaaggcggcg ggcgctgata
     2041 tgttgcgcgg cggagcttat aagccgcgta cgtcgccgta cgactttcag ggtctcggcg
     2101 tcgaagcgct gcgatttttg aaacacgcga gccgcgagac cgggttaccg attgtgactg
     2161 aagtgatgag tgaaaccgac gtggagatcg tggcggagta cgctgacgtt ttgcaggttg
     2221 gggcacgcaa catgcagaac ttttcgctgt tgcggaaatt ggcgccggtg tcgaaaccga
     2281 tattactgaa acgtggacca tcggcgacgg tgaaggagtg gttgctggca gcggagtatt
     2341 tgttgtcagg cggaaatgcg aacgtgattt tatgcgagcg tggtattaag acgtttgaaa
     2401 cggcgacgcg caacacgttg gatcttgcgg ctgtggcgct ggtgaaggag ctgtctcacc
     2461 tgcctgttct cgccgatccc tcgcacggga cggggatgcg gagtttgatc acgccgatgt
     2521 cgaaagccgc ggcagcggcg ggtgcggacg ggttgttgat tgaagttcac ccgtgtccgg
     2581 aacgcgcgct ctcggacggt ccgcagtcgt tggatctcgg tggttttgca aacctgatga
     2641 acgagctcgg caaacaaccc ctgcgcgacg taaaagcggc ataaggcgga gagcgccgaa
     2701 ggcgcagcaa gaaggtagcc aggggcaagc gcgaagcgcg cagcctggat ctgttgaaaa
     2761 cagaattgca ggcccggagg ggccgacaga aatgggcggc acgcgtgtct atcgcccctc
     2821 cggggctcct tcgccgttta ttggccgcca ccttcgtctg cgcctgcggc gctgaacgac
     2881 gcccgagtgt tcaaacgcac aaacaaacct acacgcctaa tccaccgacg tgatctggtc
     2941 gggggtgagg agcgtcaagc tgcacgcgcc gggtttgcac tgcaacgtac cacgacggac
     3001 gatctttgtc ggcgcactgt cgggaaatac caacggatac tttaccgctt tcaattgtga
     3061 cgccagcggt ttcaaactat ccgccccttt tatgaacttc acttcaacca cttgtgcttt
     3121 gcgtgtcgcg tcgggcgcaa acacgagata aaactccgct tccgccggct ccttcaggtt
     3181 cgccaccatc gcgcccacat ccagcttgtt atagttcgcc aaatctgact ttgcggtctg
     3241 caacaacttc tccaccgcat cagcactgac cagcttcagc aaactctcac gcgcctcggg
     3301 cactacgcga tacgcaactg caccctgcgc gtacattcga atcgcttctt ctttgttccc
     3361 gcgtttctcc gcaatcgcgc ccatgtggtg gcccacttcg ctgtactgat gaattaacca
     3421 cgccgcgctg atgtatctct ccgcggcgtc gagatcgcct ttttgaaagt agacccaacc
     3481 aagcgtgtcc caataggcgc ccagcgacgc agcatcgccc aaatcgtcca tcgtcaaatt
     3541 gctcgcttcc acattccgca agctgctcgc aatcgacgtt acggccgact ctgcatattg
     3601 cagtgcctta tccaattgca gtttctgagt cgcgagaaag taagcaatgt cgttcaggac
     3661 cagtggacta cgatcgagct tgagcgcctc ttcaaaagcc ttaatcccct tctcttcttc
     3721 gccgaggttc agatacgccc ggcccagggc gacgtgaagc atctcgttct ccggagcaag
     3781 cgagatcgcc tgctcgagct ccggcacagc ctctttgtat ttgcgccaat caaccagcat
     3841 ctgccccagg ttcgaacggg ccgcctggtc caacggtgtc acttcaagct gtttgcgaaa
     3901 cgcttgttca gcgttggcgt aatcctgctg ctgccagtag acgctaccca gcagatcgta
     3961 agtgtgctct tcaaaaggat tgatcttcgt ctgctcgcgc aggacttcga cggcttccgc
     4021 taatttgcgt tgcgcagcaa gcaccggccc taaatcacgc cgaacggttt tatgcttcgg
     4081 gtccttttcc aaaactcgtt tcagcaactg ttcggcaacg acgaagttct gattactcat
     4141 cgccacttgc gctgcctgaa gcaggtcggc ggtcttgacg gtgtcggaaa gcgccggtgc
     4201 gccggcggct tctgtctcga gctgaagcgt ctgtgctgca tcggaacgcg cggcggcagc
     4261 gaaggcttga tagtcctgca aacgctcggc gggcagttcg cgctgacgca gacgaagcgt
     4321 tcgctcaccg atcagggttt gtccttcgag tttgtaactt gctgcatact cgccatagtc
     4381 cctcgtcact ttcagcggca acggcaagcg agcctgatac ttggcgggca gcgtcagctt
     4441 cagcgaatag gtaatgtcaa tcggcggtcc aagttcgagc ggcttcttgc tggtcggttt
     4501 acgaccggtg aacggcggca agctgaacgg cggaaacgga agttgaagct tcactttctt
     4561 gctggaccag tcgagaaagt cgttgcgtgt tacattgaac tcaatctcaa acggtttcgc
     4621 gaggtccgcg ggatccgttg ctttaacgtc cgtgatgtcc ccctcaatgc ctccggccat
     4681 gcccagataa aagccaaggt ttttccagtt gctcttcggg gtgcggcgaa agatgaaacg
     4741 cataaacatc tccgcatcgc cgtgcaaaat gagatgcgcg tgcgccgtga tcttgccgag
     4801 ttcatcgacc gtgccttcaa ccttgaccac ttcagacgag agaaacggcg gttccgcggg
     4861 cgtcgtctca agacgtgccg gaccagtcgt aggaatcagc aacgccttct tgttgcgcag
     4921 acgcggattc aacaaaccta ccggcgcgat ttccgccgtt gtgtcagccc agagcgtctc
     4981 gttgcctaac ggaatcgcgc tgatcacgtg gtcaaactgt cccggcgaag gaatgtcgac
     5041 gtcgatctta cggaaggaat tcatcaacac cggatacgcg cgcagtccgg tcgcagccag
     5101 catcgacgcc agtaaggtgt gtttgtcttt gcagtcgcca tactggttga ccatcacgtc
     5161 ggtcgcagcg tggggctggt atcgaccctg gcccaacgag agactgacgt aacggaaatt
     5221 cttggcgacg tattcataca aagcctgcgc cttttctttc tctgtagtcc gtccttttat
     5281 taactcctca gctttgactt tgatcttttc gtccggcacg acgcgatcgc gttgcagtgc
     5341 cgcataccac tggccgactt cgtcccagct ctgaaaagtg gtaagttgca catcgggtcg
     5401 aacctcttca ggttccaccg cgtcctcttt ctcttcctcc tcttcgccct ctttaggttt
     5461 ttcttcgctg tcgggtttta ggttcgcgtg cttccaggag tagatgcgac gcccgtcttg
     5521 ttctctggtg gtgggttcat atccaggttg ggtcttcaac ttcaccttgc tctcggccgg
     5581 aatattaacc gtcaactcgt cgctgagaac gatcatccct ttcttgatga agtcgtgctc
     5641 gacccagaat tggtcttggg ccagcgccgt atgaacggtc acgactatgt gatactcgag
     5701 cacgtcgccc ggccgcagac cgggaacggt gatgtgcttg tgtcgcaggt cggtgtagac
     5761 gggcgcttca cgagcgacgg gcgcactcag gtcctgaacg tcagtcgcag cggcgttgac
     5821 cacggttccg tccgcttttc gcacacgcac gaaatccatg tcgagctt
//
LOCUS       DASTEY010000032         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_247861, whole genome shotgun sequence.
ACCESSION   DASTEY010000032 DASTEY010000000
VERSION     DASTEY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247861"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFO72_00590"
     CDS             complement(<1..373)
                     /locus_tag="VFO72_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477808.1"
                     /translation="MLLIVLFLFIGSESVALAQNRSVYTSLEAKRCRTIKTETTGAGD
                     YEGRCRGVAGYSLTLLEGDLRQNIIVTTPKGTKHSLELWNVISGGFSYVGPNAEWRTT
                     RKKGRLTPVALIVRYNASEDAE"
     gene            complement(379..1857)
                     /locus_tag="VFO72_00595"
     CDS             complement(379..1857)
                     /locus_tag="VFO72_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9477809.1"
                     /translation="MATYRNFINGKWVESVSRRTASNINPADTGDVLGTVRQATREEA
                     RTAVEAAANAFRSWRATPAPARGRIVAQAARLLEENKEEIAQLLTREEGKTIAESRGE
                     LQRSINVAEFCAAEARRLTGETIPSELPLNFAYTIKQPIGVVACVTPWNFPVAIPVWK
                     IAPALVAGNTVVFKPASITPATAVRITEIFEAAGIPPGVLNLVLGSGSEAGDEIINHQ
                     AVKAISFTGSNGVGIRLYEQASRRGAKVQCEMGGKNPVIVLEDADMDLAVESTAQGAF
                     GSSGQRCTATSRAVVVKEIADEFVARVVKRADSMRLGAGSDPQTEMGPSVDENQFKTV
                     LSYIDIGREDGATLVCGGSRASGEGLDKGYFVRPTVFDHVTPDMRIAREEIFGPVLSV
                     LRVRDFEHAVEVANDCEYGLSSSIFTNDAARIFRFVDEIETGMTHINSPTTGGEAHIP
                     FGGIKGTGIGDREQGSTALDFYTDLKVVYVDYTGHKREGNLY"
     gene            1896..3389
                     /locus_tag="VFO72_00600"
     CDS             1896..3389
                     /locus_tag="VFO72_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9477810.1"
                     /translation="MTVEPQSIPELLHQRASVAPDKVFLISEADKRQFTYREFESGVG
                     RAAGMLAAHGIGKGDVVSLLMPNSVEYIVAYFACWQLGALAGPVNGLLKAQEIAYVVS
                     NSEARLMLVNSEFLPVVEGIQVPRVVFDDEREASHGFDGRSTDEVRQEDEAIIIYTSG
                     TTGKPKGCLLTHGNVMANAREISSWLGFTEKDRLLTIMPLFHMNAVSVTTMSALYAGG
                     STVVSPKFSASRFWQIISDYQITSFGSVATMLSMLLSTYPKGVPAGLKTDQLRFAMCG
                     SAPLPAEILRRFEETFNCLVIEGYGLSESTCRSTFNPPDQRRRPGSVGLPIGNEMRVV
                     DEEDRDVPDGQLGEIVLRGENILKGYFKNEAATATAFRNGWFHTGDIGYRDADGFYYI
                     VDRKSDMIIRGGENIYPREIDEVLYQHPQIAAAAVIGIPDELYGEDVAAVVVPKSGAK
                     ISEQDVIDFCKARLADYKCPKTVRFVNDIPKGPTGKLLKRELARLFG"
     gene            complement(3343..>3701)
                     /locus_tag="VFO72_00605"
     CDS             complement(3343..>3701)
                     /locus_tag="VFO72_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477811.1"
                     /translation="NGALLGLAAAVAFSLGWARFGHLINVRRFFQVTGIFLLLFMIQV
                     AIYSFHEFTEAGLLPNSDYLHEATEKFSPDGLYGKWFSPLMISICALWLFGAWLIDRT
                     KLSQTAAPVPSSATSQ"
BASE COUNT          820 a         1023 c         1037 g          821 t
ORIGIN      
        1 tctcagcgtc ctcactcgcg ttgtagcgga caatcagagc gactggcgtc agtcttcctt
       61 tcttccgtgt cgtgcgccac tccgcgtttg gtccaacata agagaatccg ccactgatga
      121 cgttccatag ttccagcgaa tgtttcgtac ccttgggggt cgtaacgatt atgttctggc
      181 gcagatcgcc ttcaagtaaa gtcagcgagt atccggcgac gccgcggcag cgcccttcgt
      241 agtcaccggc accggtggtt tcggttttta ttgtgcgaca ccgctttgct tccaaagaag
      301 tgtagacgct gcggttctgc gcgagggcca cggattccga gccgatgaat aaaaagagta
      361 cgattagtaa cactaatctc aatacaagtt tccctctcgt ttgtggccgg tgtagtcgac
      421 atacacgacc ttcaggtccg tgtaaaaatc cagcgccgtc gaaccctgct cgcgatcgcc
      481 gatgccggtg cctttgattc ccccgaacgg aatatgggct tcgccgcccg tcgtcggaga
      541 gttgatgtgc gtcatcccgg tttcgatctc gtcgacgaag cgaaagatac gcgccgcgtc
      601 gttcgtaaag atcgacgacg acaaaccata ttcacaatcg ttcgccacct ccaccgcgtg
      661 ctcgaaatcc cgcacacgca acacagacag cactggtcca aagatctcct cgcgcgcgat
      721 tctcatatcg ggtgtcacgt gatcgaaaac cgtcggccgt acaaagtaac ccttgtcgag
      781 tccttcgcca gacgcacgtg agccgccgca aacaagcgtg gcgccgtctt cgcggccgat
      841 gtcgatgtag ctcagcacgg ttttaaactg attttcatcg acactcggcc ccatctccgt
      901 ctgcggatca gatccggcgc cgagacgcat cgagtcggca cgcttaacaa cacgcgcgac
      961 gaactcgtcc gcaatctcct tcacgactac cgcacggctc gtcgctgtgc aacgttgacc
     1021 tgacgaacca aacgcgccct gagccgtcga ttccactgcc aaatccatgt ccgcatcttc
     1081 cagtacgatg accgggttct tccctcccat ttcacactga actttcgcgc cacgccgcga
     1141 cgcctgctcg tagagtcgaa tgccgacgcc gtttgaaccg gtaaacgaga tcgctttaac
     1201 tgcttggtgg ttgatgatct cgtcaccggc ttctgaaccg gatccgagaa ccagattcaa
     1261 aacacctgga ggtatgcccg ctgcttcgaa gatctccgtg attcgcacgg ccgtcgcagg
     1321 agtgatcgat gcgggtttga agacgactgt atttcccgcc acgagcgccg gcgcgatctt
     1381 ccaaaccggg attgcgacgg gaaaattcca cggtgtgaca caagcgacca ccccgatcgg
     1441 ctgcttgatc gtgtacgcga agttcaaagg caactctgag ggaatagtct cgccggtaag
     1501 acgccgtgct tcggcggcgc agaactcggc gacgttgatc gagcgttgca gttcgccgcg
     1561 cgattcggca atggtcttac cttcttcgcg cgtgagcagt tgcgctatct cttccttgtt
     1621 ttcttcgagt agtcgcgccg cttgcgcaac gatacggccg cgagccggcg ccggcgtcgc
     1681 acgccaactg cgaaaagcat tggcggcagc ctccacggca gtccgtgcct cctcgcgtgt
     1741 agcctgtcgt accgtgccta aaacatcacc cgtgtcggcc gggtttatgt ttgaggctgt
     1801 tcgcctagag accgattcaa cccatttgcc gttgatgaaa ttgcgatagg tagccatagg
     1861 agtgctggaa tatatcatgt gctataacac tttgcatgac tgtggaacca cagagtattc
     1921 ccgaactttt gcaccaacgg gccagtgttg cacctgataa agtcttcctg atttcagaag
     1981 cagacaagcg acagtttacc tatcgggaat tcgaaagtgg agtcggtcga gcggcgggga
     2041 tgctcgccgc gcatggcatc ggcaaaggcg acgtagtgag tctgctgatg ccgaacagcg
     2101 tcgagtacat cgtggcatat tttgcgtgct ggcaacttgg cgcgcttgcg ggaccggtaa
     2161 atggtttact caaggcgcag gagattgcgt acgtggtctc gaactctgag gccaggttga
     2221 tgttggtgaa ctcggagttt ttgccggtgg tggaggggat tcaggttccc cgggtggttt
     2281 ttgatgatga gagggaagcg agccacggat ttgacggacg cagcacggac gaggttagac
     2341 aagaagacga agcgatcatt atttacacct ctggaactac cggtaaacca aaaggttgtt
     2401 tactgacgca tgggaacgtc atggccaacg cgcgcgagat cagttcctgg ctcggcttca
     2461 cggaaaagga tcgtctgctc acgattatgc cgctgtttca catgaacgcg gtttccgtca
     2521 caacgatgtc agcgctttat gccggcggtt caacggtcgt gagtccaaag ttttccgcct
     2581 cacgtttttg gcagatcatt tcggactatc agatcacttc atttggttcc gtagcgacaa
     2641 tgttgtcgat gcttctcagc acgtatccga aaggcgtgcc cgcgggactg aagaccgatc
     2701 agttgcgctt tgcgatgtgc ggttcggcgc cgctgccggc cgagatattg cggcgcttcg
     2761 aggagacgtt caattgtctt gtcatagaag gttatggtct atcggagtca acctgtagat
     2821 cgacgttcaa tccacccgac caacgccgcc ggcccggttc ggttggtctg ccgatcggca
     2881 acgaaatgcg ggtcgttgat gaagaagatc gagacgtacc ggatgggcag ttgggagaga
     2941 tcgtgctgcg tggcgaaaac atcctcaaag gctatttcaa aaatgaagcc gcaacagcga
     3001 cagcgtttcg caacgggtgg tttcacaccg gcgacatcgg ttatcgcgac gcagatggtt
     3061 tctactacat cgtcgatcgc aagtcagaca tgataattcg cggcggcgag aacatctatc
     3121 cgcgagagat cgacgaagtc ttgtatcaac atccgcagat tgccgcggct gcggtgattg
     3181 gcattccaga cgagctctac ggcgaagacg ttgccgcggt ggttgtaccg aagtccggag
     3241 cgaagatctc agaacaggac gtaattgatt tttgcaaagc gagactggcg gactacaagt
     3301 gtccaaagac agttcgcttc gttaacgaca ttcccaaggg tcctactggg aagttgctga
     3361 agagggaact ggcgcggctg tttggctgag ttttgttcga tcgatcagcc acgcgccgaa
     3421 aagccacagc gcacagatcg aaatcatgag cggtgaaaac cacttgccgt aaagaccgtc
     3481 gggcgaaaac ttctccgtcg cttcgtggag gtagtcgctg tttggcagca accccgcctc
     3541 ggtaaactcg tggaacgagt agatagccac ctgaatcatg aacagtagca gaaagattcc
     3601 agtgacctgg aagaagcgcc gcacgttgat gagatgaccg aaccgagccc agcctaacga
     3661 aaacgcgaca gccgcagcga gaccaagcag cgcgccgttg a
//
LOCUS       DASTEY010000033         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_264278, whole genome shotgun sequence.
ACCESSION   DASTEY010000033 DASTEY010000000
VERSION     DASTEY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264278"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..770
                     /locus_tag="VFO72_00610"
     CDS             9..770
                     /locus_tag="VFO72_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9477812.1"
                     /translation="MSDATITLSPTENVIKSDIQVDGHDPSMLISMRNIWKTYQMGAE
                     KVHALHGVSFDIPRGEYIAIIGPSGSGKSTLMNLIGCLDTPTQGQYWLNGKNVSEMDD
                     DELARIRNQEVGFVFQTFNLLARATALHNVELPLIYGGIGSAQRHEMARQALSAVDLS
                     DRITHRPNELSGGQRQRVAIARALVNHPSILLADEPTGALDSHTSMEIMDLFERLHSE
                     GNTIIVVTHEHDIAARAHRVISIRDGQIEKDERIR"
     gene            985..2325
                     /locus_tag="VFO72_00615"
     CDS             985..2325
                     /locus_tag="VFO72_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477813.1"
                     /translation="MTVRSGEHDTKPQRYTFARAVLPARIKSFPLFRLAAIWEAFRVA
                     TASLRANKLRTALTLMGIIVGVTAVIAVVTIIKGLDQTVAQTFSSQGSTVFTISKNPQ
                     IITSREDFIRFNRRKDVTHEDAAAIARLCTACWRVGIAANAIETVKYQDQKAENVRVR
                     GVEPLTMFDIDGVSLEVGRIWSETEGASGREICVVGPDMLKNLFGDAPAEQAVGKEIR
                     IDGRPYLVLGVLEPLGSIFGFSRDNVVYIPYSTYQKAYGARRSLVVFIQVPAAEQLEV
                     AEDQVRTIMRNRRGLAIGNVEDEGFSLETQDVFLNLYGSATSNIYIVTIGVAAISLVV
                     GGIVVMNIMLVSVTERTKEIGIRKAIGARRKDILTQFLIEAVVVTAMGGAIGVGTGFG
                     LAYVISALIGFPLLVSVASAVLGVGVSSVVGIVSGLWPAWRAAKLDPIEALRAE"
BASE COUNT          516 a          672 c          675 g          498 t
ORIGIN      
        1 gctagctaat gagcgatgca actatcacgc tttcaccgac agaaaacgtg atcaagtcag
       61 atattcaggt tgacggccac gatccgtcga tgctgatctc gatgcgcaac atctggaaaa
      121 cctaccagat gggcgccgaa aaagttcacg cgcttcatgg cgtgtctttc gatattccgc
      181 gcggcgagta catcgcgatc atcggcccgt ctggctcggg taaatcaacg ctcatgaacc
      241 ttatcgggtg tctcgacacg ccgacgcaag gccaatactg gctcaacggc aaaaacgtct
      301 ccgagatgga cgacgacgag ttggcgcgca ttcgcaacca ggaagtcggc ttcgtcttcc
      361 agacattcaa cctgctcgcg cgcgccactg cgctgcacaa cgtcgaactg ccgttgatct
      421 acggcggcat cggctcggcg caacgacacg aaatggcgcg ccaggcgctc tccgcagtcg
      481 acctttcgga tcgcatcacg cacagaccta acgagctttc cggcggccaa cgccagcgcg
      541 tcgccatcgc gcgcgcgctc gtaaatcatc cctcgattct gctggccgac gaaccaaccg
      601 gagcgctcga ctcccacacc agcatggaga tcatggatct cttcgaacgg ctgcattccg
      661 aaggcaacac gatcatcgtc gtcacgcacg agcacgacat cgccgcacgt gcccaccgcg
      721 tgatttcgat tcgcgacggc caaatagaaa aagacgaacg aatcaggtag gagaaccggc
      781 tttgtcctca gagtcatctc attctcaccc ggcttcagcc gggtgtcgtg ctttctgtag
      841 ggcagaaaac cgtttcaacg gtttttctca ccggttcaca tggaaaccgt tggaaacggt
      901 ttccgaatag gagctttgcc aagacacccg gctaaagccg gttgagaatg aaatgacttt
      961 tcaggcaagg ttaggagaaa caggatgacg gtgcgaagtg gcgagcacga cacgaagcca
     1021 cagcgctaca cgtttgctcg cgctgtcctc ccggcgcgaa tcaagtcgtt tccgcttttc
     1081 cgcctcgccg caatctggga ggcatttcgc gtcgccaccg ccagcttgcg cgcaaacaag
     1141 ctgcgaaccg cgctgacgtt gatgggaatt attgtcggtg tgaccgcggt gatcgcagtc
     1201 gtaacgatca tcaaaggtct cgatcaaacc gtggcgcaga ccttctcttc gcaaggctcc
     1261 actgtcttca cgatttcgaa aaacccgcag atcatcacgt cgcgtgaaga cttcataagg
     1321 ttcaaccgtc gcaaggacgt gactcatgaa gacgcggcag ccatcgcgcg gctctgcact
     1381 gcctgctggc gtgttggcat tgccgcaaat gcgatcgaaa cggtgaagta ccaggaccag
     1441 aaagccgaga acgttcgcgt tcgcggggtc gagccgttga ccatgtttga catcgacggc
     1501 gtgagccttg aggtcggacg catttggtcg gaaaccgaag gcgcgtcggg ccgcgagatc
     1561 tgcgtcgttg gtcccgacat gttaaagaac ctctttggcg acgcgcctgc cgagcaagca
     1621 gtcggaaaag agattcgaat cgacggccgc ccgtatctcg tgctgggtgt gctggaaccg
     1681 ctcgggtcga tattcggctt ctcgcgcgac aacgtggtct atattcctta ttcgacttac
     1741 cagaaagctt acggcgcgcg gcgttcgctc gtagttttca ttcaggtgcc cgcagcagaa
     1801 caactcgaag tggccgaaga ccaggtcagg accatcatgc gaaaccgtcg cggcctcgca
     1861 atcggtaacg tcgaggatga aggcttctcg ctcgagacac aggacgtgtt tctgaatctc
     1921 tacggctcgg cgacttcaaa catttacatc gtaaccattg gcgtagcggc gatatcgctc
     1981 gtcgtcggcg gcatcgtcgt gatgaacatc atgctcgttt ctgtaacgga acggaccaag
     2041 gagatcggca ttcgcaaagc gattggcgcg cgtcgcaaag acatcctgac gcagtttttg
     2101 atcgaagccg tcgtggtgac ggcgatgggc ggcgcaatcg gtgtcggcac cggttttgga
     2161 ctggcgtacg tgatctcggc cctgatcgga tttccattgc tggtgagtgt ggcttcggcg
     2221 gtgttgggag ttggagtttc gtcggtagtc ggcatcgtga gtggcctatg gcctgcctgg
     2281 cgtgcagcga agttggatcc aattgaagct ctcagagcag aatgaaggca ttgccgattt
     2341 tcgattgccg attgccgatt t
//
LOCUS       DASTEY010000034         4716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1170740, whole genome shotgun sequence.
ACCESSION   DASTEY010000034 DASTEY010000000
VERSION     DASTEY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170740"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFO72_00620"
     CDS             complement(<1..387)
                     /locus_tag="VFO72_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477814.1"
                     /translation="MSDTLGITRNKFFGPFLWVVTGIGCLSFLYAFSRIDLGQIDSRF
                     AVLVAMALLLTSRITVPIPRLSSQISVSDTFVFLVLLLYGTPAAIIVGGVEALLSSLR
                     FSRKRSIVAFNWASAAVSIGITGGLLE"
     gene            complement(454..1311)
                     /locus_tag="VFO72_00625"
     CDS             complement(454..1311)
                     /locus_tag="VFO72_00625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9477815.1"
                     /translation="MDAISVDRLEQGDEAEVLEFLAQRPIHTVAMMSLIHDNGLVSPL
                     NRGTFYGCRDLNGRLEGVALVGHATLTETLSDRALAALAQVAQQCSNTHLIMGEKERM
                     ADFWSHYAAAGRQQRLACREWLFELSWPIEARGRVEELRPATVAELDLVMPVQAALAF
                     AESDINPMQADPRGFRARCLRRIEQRRTWVVIENGKLIFKADVISRTREVNYLEGIWI
                     CEERRADGAGLRFMSELMRRLLEDTKSICLLVNETNKHAQSFYRKCGFHFRATYETIF
                     LPRMEPAQN"
     gene            complement(1425..3602)
                     /locus_tag="VFO72_00630"
     CDS             complement(1425..3602)
                     /locus_tag="VFO72_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9477816.1"
                     /translation="MPDGTVPAQRVSPTSVGGIQKTPQLTEMTTVKSMKLTKRFLTLA
                     LLANLSLGSTLASGIAMAATRGGKGKNASQNGKGAKHNKIASELRDQLDGDSGSSRVK
                     VILQLNNKPSGSLTALLASNGVKIRKHFTSLNSFALELPLSVVNVLSQFSEVLFVSVD
                     SEVRTLGGHVAHTSGADNVRAMSADGALDGNGIGIAILDSGIYGEHTSFIDIQTGQSR
                     VVVNLDFTGESRTDDPYGHGTHVAAAAAGNGIVARGEYIGIAPRARLINLRVLNSRGI
                     GSVSSVMSALDWLMLHGSNYNVRVANLSLGMPAITSYQLDPLCVAVRKLVDRGMVVVA
                     AAGNNGKNSVGQKVYGQVHSPGNAPSAITVGAVDTRGTDTRADDAIATYSSRGPTRSF
                     WTDENGVNHYDNLIKPEISAPGNKTIFAQAPNNYLVTQNPSLDVSVSNNPTRSQMMLS
                     GTSMAAPLVAGAAALMFEANPTLTPNLLKSLLMYTAQQLPNFNTLEQGAGELNIDGAI
                     RLAKLVRTTIDSSTPLGAPLLISAPPAPETRISIAGGATTFSWAQGIILGPTYATGAA
                     LITEYQKIYATGVLLGDGVLLGDRALIGDGVLLGDGVLLGDGVLMGDATGVLMGDYTM
                     LSSGVLLGETIHTSDGTQFGYGLDFLATGVLIGDGVLIGDGVLIGDGVLIGDGVLIGD
                     GVLIGDGVLIGDAILVAFNSLLNGDPTTSMATVPDDERDYLGY"
     gene            3711..4538
                     /locus_tag="VFO72_00635"
     CDS             3711..4538
                     /locus_tag="VFO72_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477817.1"
                     /translation="MIEFGHMLPEEIEVHRDRMWRRDPEYRVEDVYAAERFIEDVGFC
                     EALTDSRRPGPSLYVAVCGRRDAHMPRNVQKDPESRLAWSIKDEVMRRGRFFYGKLIK
                     GRSTFITPRLLPFFNALWGVPRRLEKEALSADAQIILKIMRDEWEMATRDLREESGIK
                     ERARFDRAMVQLQKTLKIIPSDVLYEPTFTYIWSIPEARFADQLKEKVKREVALREIA
                     RAYLTGAGMTFRGELARVTGLRSSEVGLGNWALVDEGFAERLGPGIYRLKNLRLNNQ"
BASE COUNT         1026 a         1274 c         1377 g         1039 t
ORIGIN      
        1 ttcgagaagg ccgcccgtga tgccgattga aactgcggca ctggcccagt tgaacgcgac
       61 aatacttcgt ttgcgactga agcgaagtga tgacaagaga gcttcgaccc cacccacaat
      121 gattgccgca ggcgtgccat aaagaagcag taccagaaat acaaaggtat cggagaccga
      181 gatctgcgaa ctgaggcgtg gaattggaac agtgatgcgc gaggttagca gcagcgccat
      241 cgccacgagc acggcgaaac gcgaatcaat ctggccgagg tcgattcgcg aaaaggcgta
      301 gagaaacgag aggcaaccaa ttccagttac aacccatagg aatggcccga agaacttgtt
      361 tctcgtaatt ccaagggtgt ctgacatctc ggtttgtgat caaggtgagg tgtctgggct
      421 accttaatca ggtcagatcg ggggaagagt gtttcaattt tgcgccggct ccattcttgg
      481 gagaaaaatg gtttcataag tcgcccggaa gtgaaatccg cacttacggt agaagctttg
      541 cgcgtgtttg ttcgtttcgt ttaccaggag gcagatcgat ttcgtgtctt cgagcagacg
      601 tcgcatcagt tcggacatga atcgcagtcc ggcgccatcg gcccggcgct cttcgcaaat
      661 ccagatacct tcgagatagt taacttcgcg agtgcgcgaa atcacgtccg ctttgaagat
      721 caacttgcca ttctcaataa ccacccaggt ccttcgttgt tcgatgcgac ggaggcaacg
      781 tgcgcgaaaa cctctcggat cggcttgcat cggattaatg tcactttcgg cgaaagcgag
      841 tgctgcctgg actggcatca ccaaatccaa ctcagcgact gtagcggggc gcagttcttc
      901 aacgcgcccg cgcgcttcga tgggccagct caactcgaat aaccactcgc gacaagcgag
      961 tcgctgctgg cgtccggccg ctgcgtaatg gctccaaaaa tcagccatgc gttctttctc
     1021 acccatgatc aggtgtgtgt ttgagcattg ctgggccacc tgggccagtg ccgccaacgc
     1081 gcgatccgaa agcgtttccg tcaacgtagc gtggccgacg agcgcaacac cttccagccg
     1141 cccgttcaag tcgcgacagc cataaaacgt gccgcgattg agtggactga cgagaccgtt
     1201 gtcatgaatc aagctcatca ttgcgacggt atgaatcggc cgttgggcca ggaattcgag
     1261 cacctcggct tcatcgccct gctcgaggcg gtcgacagaa attgcgtcca cctttggcgc
     1321 cagtttgtgg ctgagatcat tcgctcctgg tcttctgcgt acgtgtaaca tgcgtctacg
     1381 tctccgacag tagttccgtg tttgtgggtt gggcatgtta ccgctcagta gcccaggtag
     1441 tctctctcgt cgtcgggaac ggttgccatt gacgtagtgg ggtcgccgtt gagcaacgaa
     1501 ttgaaggcga ccagtattgc gtcgccaatc aggacgccgt caccaatcaa aacgccatcg
     1561 ccaatcagga cgccgtcacc gatcaggacg ccatcaccaa ttaggactcc gtcgccgatg
     1621 agcactccgt caccgatcag cacgcccgtg gccaggaagt ccagaccgta gccaaactgc
     1681 gtgccgtcgc tcgtgtgaat cgtttcgccg agcaggacac cactggagag catggtgtag
     1741 tcgcccatga gcacgcccgt ggcgtcaccc atgagcacgc catcgccgag cagaacaccg
     1801 tcgccgagaa gcactccatc accgatcaaa gcgcggtcgc caagtaacac gccatcacca
     1861 agcagtacgc ccgtggcata gatcttttga tattcggtga tcaaagcggc gcccgtggcg
     1921 taagtcggtc cgagaataat gccctgggcc cacgagaacg tggtggcgcc accggcaata
     1981 gagatgcggg tttcgggagc cggaggcgcg ctgattaaca acggggcgcc cagcggcgtg
     2041 gagctgtcga tcgtggtgcg aacaagtttg gcgagacgta ttgcgccgtc gatgttcagt
     2101 tcgccggcgc cctgttccaa cgtgttgaag ttgggcagtt gctgggctgt gtacatcaac
     2161 aacgacttca acaagttcgg cgtcaatgtc gggttagctt cgaacatcaa agctgccgca
     2221 ccagcaacca gcggcgcggc cattgacgta ccgctaagca tcatttgact gcgtgtcggg
     2281 ttgttgctaa cgctcacgtc gagcgagggg ttctgggtaa cgagatagtt gttgggagcc
     2341 tgggcaaata tagtcttatt accgggagct gagatctccg gtttgatcag attgtcgtag
     2401 tggttgacgc cgttttcatc cgtccagaag ctgcgcgtcg gcccacgaga actgtacgtt
     2461 gcgatcgcgt cgtctgcgcg cgtgtctgtt ccgcgcgtgt caactgcgcc aacggtaatc
     2521 gctgatgggg cgttgccggg tgaatgaact tgaccgtaaa ccttctggcc cacgctgttc
     2581 ttgccgttgt tgccggcggc ggcgacaacg accataccgc ggtcaacgag tttgcgaacg
     2641 gccacgcaca gcggatcaag ctggtaggaa gtgattgccg gcatgccgag gctcaggttg
     2701 gcaacgcgca cgttgtagtt cgaaccatgc agcatgagcc agtcgagcgc cgacattaca
     2761 cttgaaactg aaccgattcc tctcgagttc aggacgcgga gattaatcag cctggctcgt
     2821 ggcgcgatgc cgatgtattc gcctcgcgca acaatgccgt tgccggcggc tgcggccgcg
     2881 acgtgagtgc cgtgtccgta cggatcgtct gtgcgactct cgccagtgaa gtccagattg
     2941 acgacaactc gcgactgacc ggtctgaata tcgatgaacg aagtgtgttc tccgtagata
     3001 cctgagtcga gaattgcgat gccgataccg ttgccatcga gtgcgccgtc cgcactcatc
     3061 gcgcgcacgt tatcggcgcc tgaggtatgg gccacgtggc caccaagagt tctgacttca
     3121 ctgtcgacgg agacgaaaag aacttcggag aattgggaca ggacgttgac aacactgagg
     3181 ggtaattcga gggcaaaaga gttcaggctg gtgaaatgtt ttcttatttt gacgccgttc
     3241 gaagctaaca acgcggtcag tgaaccgctc ggcttattgt tcagttgcag gataaccttc
     3301 acgcggctgc tgccactgtc gccatcgagt tgatcgcgaa gttcagacgc gatcttattg
     3361 tgtttcgcgc ccttgccgtt ctgcgaggcg ttcttgcctt tgccgccacg ggtggcggcc
     3421 atcgcaatcc cgctcgcgag ggtggatccc aacgagaggt ttgccagcag tgccagcgtc
     3481 aggaaccgtt ttgttagctt catcgatttc accgttgtca tctccgtcaa ctgaggtgtc
     3541 ttctgaatgc cgcccacaga cgtgggcgaa accctttgtg caggcaccgt gccatctggc
     3601 atggaaacag ttacatcgtt gtgcagcaac cgtttaggtc gattgccggg catctgaagc
     3661 agatttggac ctgaccgatc agggcagttt gaccgctcag ctaaatcact ttgatagaat
     3721 tcgggcacat gcttcccgag gagatagagg tccatcgcga tcgtatgtgg cgacgcgatc
     3781 cggaataccg tgtcgaggac gtttacgcgg ccgaacggtt catcgaagac gttgggttct
     3841 gcgaagcttt gaccgactcc agacggcctg gaccatcgct ttatgtggct gtttgcggcc
     3901 ggcgtgacgc ccacatgccg cgaaatgtgc agaaggatcc ggagagtcga ctggcgtggt
     3961 ctatcaaaga cgaggttatg cgtcgcggtc gttttttcta cggcaagtta attaaaggac
     4021 gatcgacgtt catcaccccg cgtctgcttc cgttctttaa cgcgctctgg ggcgtgccgc
     4081 gccggttgga gaaagaggcc ttatctgctg acgcgcaaat tattttgaaa attatgcggg
     4141 atgagtggga aatggccact cgcgatctac gtgaagagag cggaatcaag gagcgggcgc
     4201 gcttcgaccg cgccatggtc cagttgcaaa agacgttgaa gatcattccg agtgacgtgc
     4261 tgtacgaacc gacttttacc tatatctggt ctattccgga agctcgtttt gcggaccagc
     4321 tcaaagagaa ggtgaagagg gaagtggcgt tgcgcgagat tgcccgcgct tatctcacag
     4381 gcgcgggaat gacgtttcgc ggtgaacttg ctcgcgtgac aggcctgcga tcatccgaag
     4441 tgggactagg aaattgggcc ctggtggatg aaggatttgc ggaacgactc ggccccggta
     4501 tttaccgtct caagaacctt cgcctcaaca atcaatgatc aggcggccac agatgaaccc
     4561 ggatgaacac tgacagtgaa aaccagcctt cttccgtggc ttatactaag gccgtgccga
     4621 agaagcacgt ccgactaaga accttgagac aactctttct tggtcaacgg aggagtcgtt
     4681 tcgcgtagca tctgttgctc gagttgggct atttcc
//
LOCUS       DASTEY010000035         7929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1450976, whole genome shotgun sequence.
ACCESSION   DASTEY010000035 DASTEY010000000
VERSION     DASTEY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450976"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..2280
                     /locus_tag="VFO72_00640"
     CDS             409..2280
                     /locus_tag="VFO72_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET9477818.1"
                     /translation="MNALPIIIGALCVIAISYRYYSAFIAAKVLALDDSRPVPSQTMY
                     DGHNYYPTNKWVLFGHHFAAISGAGPLIGPVLAAQFGFLPGLLWLVIGVCLGGAVHDF
                     MIMVGSVRRKGKSLAEMARTEISPLSGLVAGVAILFIVVIALAGLGLVVVNALAESPW
                     GTFTVGFTIPLALFMGLYMYRFRKGKIVEASIIGIVGLLFAVYLGGRIAESPAAEAFT
                     LSRNSLIVIMAAYGFIASVLPVWMLLCPRDYLSSYLKIGTIAFLILGVIIVAPDLNMP
                     AFTPFTAGGGPVIPGKLYPFVFITIACGAISGFHALISSGTTPKMIAKETDTRMIGYG
                     SMLMEGIVGIVALIAAASLFPGDYFAINTAQGTEAQKSAYVRMVDTHSSEGFNLQPQE
                     IDRLEQESGEKNLRGRTGGAVTLAIGIAKIFAGIPGLGGFMKYWYHFAIMFEALFILT
                     TIDTGTRVARFLVGEFGGRFYRKLEEPNWMPGSVITSALVVSAWAAFIWSGSISTIWP
                     MFGIANQLLAAVALCVATTILVNTGKAKYAWVTIVPLSFVSTTTLVAGFKSITDIFWP
                     LAQKPETSTQGLINTGLTVIIMFAAVVIMIDSIRRWLGTRKGPEVSQIPEPLFEKSV"
     gene            2351..4879
                     /locus_tag="VFO72_00645"
     CDS             2351..4879
                     /locus_tag="VFO72_00645"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET9477819.1"
                     /translation="MELSLLLRRAAIAIAAMLTTLAFGLPTFAQQPHRPGGEANLQLP
                     NLTEVNFLNNSISGHNLLLFGIAICVLGLLFGLAMYTQLRKMPVHSAMREISELIYAT
                     CKTYLITQGKFILILEAFIAVVIVLYFGVLSEMEPFRVLIILFFSLVGIAGSYGVAWF
                     GIRVNTFANSRTAFASLQGKPFPIYAIPLKAGMSIGMMLISVELLIMLCILLFIPGDY
                     AGPCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCT
                     GDNAGDSVGPSADGFETYGVTGVALITFILLGVPNPIVQVQLLVWIFVMRIMMIVASA
                     LSYFVNDAIAKARFRTADSMNFEAPLTSLVWITSIVSVVITYVVSWLTIPTLSGNPTL
                     WWKLATIISCGTLAGAIIPELVKVFTSTESRHVSEVVTSSREGGASLNILSGLVAGNF
                     SGYWLGISIVALMAIAYFFTNLGAPAGLGLGALMVADVAAPVFGFGLVAFGFLGMGPV
                     TIAVDSYGPVTDNAQSVYELSLIEQVPNIEEEVNKEFGMTVNFGRAKHLLEENDGAGN
                     TFKATAKPVLIGTAVVGATTMIFAIIMALTDGLRTNIESLSLLHAPFVLGLITGGAII
                     YWFTGASIQAVTTGAYRAVEFIKANIRLEGVERASVEDSKKVVQICTQYAQKGMFNIF
                     LTVFFSTLAFAFVEPFFFIGYLMSIAVFGLYQAIFMANAGGAWDNAKKVVEVDLKMKG
                     TPLHDATVVGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVELAVSLTARNNGDTMLTH
                     ILALAFFLVSVFVLWRSFHGMRIGTMALEPRPSEREVVKSAAGD"
     gene            4886..5479
                     /locus_tag="VFO72_00650"
     CDS             4886..5479
                     /locus_tag="VFO72_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET9477820.1"
                     /translation="MNTDVRADTLLGAALAPIDWTRSVGLVVVFSLFIAAAAQFAIHI
                     GPIPITGQSFAVLLTGALLGSRLGAAAVIAYLIEGAVGLPFFAPGGAPGILRFFGATG
                     GYLVAFPAAAFVTGAFAEHGWDKNYPQAVAAMAIGSVIILLGGWAWYSVLTNTPPIAA
                     FQIAVWPYLAGDLIKICLAAAVLPTGWALLKRKASGK"
     gene            5549..5941
                     /locus_tag="VFO72_00655"
     CDS             5549..5941
                     /locus_tag="VFO72_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9477821.1"
                     /translation="MYVLKVKSRQREELVEFTDLVRDKLKESGAREGIVVLYVQHTTA
                     GLTINENADPDVPRDMLHALRTLIPQHGMGFRHGEENSDAHIKTSLVGTSVTVPFKDG
                     ELLLGRWQGIFLCEFDGGRERQVIMMVR"
     gene            complement(5998..6276)
                     /locus_tag="VFO72_00660"
     CDS             complement(5998..6276)
                     /locus_tag="VFO72_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477822.1"
                     /translation="MSEENNSARDLDFEQARLAYSIIENLVEHTRIVSDLIALMAQVL
                     DEDTTKALTQTPTWTAYLDSRRAMENTKRDIEKFAEILNQLKPDDLNS"
     gene            complement(6281..7258)
                     /locus_tag="VFO72_00665"
     CDS             complement(6281..7258)
                     /locus_tag="VFO72_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET9477823.1"
                     /translation="MTEATAIHSNEMAEYAAVRDGSVGLIDLSTRGRISVSGSEAVMF
                     LNGLITNDMKTLEENRWMPAAFPNVQGRLLAAVRVIRRAGDILIDTEAITRDKVLKII
                     ERFTLAGDFRVRDLTEETGQISLQGRGAGELVEKIFGVSDLPRDGVWQDGGVTIIRAT
                     HTGEDGVDVIADSGFVKTFWATPVSEGVAEILRIEAGIARYGRDMDETNVVTETNLDD
                     AVSYTKGCYVGQEIIVRIRHRGHVAKKLTGLLFETDAKIEAGAVIKSTDDKEIGRVTS
                     TTFSPKLAATIGLGYVRYEHLAPGTSIIVDGVPATTHSLPLIRGSWYQD"
     gene            complement(7255..>7929)
                     /locus_tag="VFO72_00670"
     CDS             complement(7255..>7929)
                     /locus_tag="VFO72_00670"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9477824.1"
                     /translation="QRRHPRPDNSDVALFWDRRKRSSLMEKSKEKLFAELVDEAAGCT
                     RCPAMCGRSAVLSKLNGSPRARIMFIGEAPGRKGADRTRVPFSGDQSGANFDRFLGSI
                     GLSRRQIFITSAALCNPRTETGANRKPARREMANCSDFLRRTIELIDPCVIVTLGSVA
                     LEALKRIQYHELNLKQSAAKIHSWQERVLVPIYHPSPQVLASHRREAEQLKDYQVVAQ
                     AVRMHP"
BASE COUNT         1510 a         2159 c         2299 g         1961 t
ORIGIN      
        1 tccgcatgac gtcgcgccgc gcgaactctt cgccgaagca ttcgatccgt cagccgagaa
       61 gcataaccag acgctttatg gtttactaat aatcatcgcc ggcctcatcg gcgcggtggt
      121 tgggatcgca ctcggacgtt cggcaacgga agcgtctgaa tagtgcggtt ttgaactccg
      181 aaggagttcg caaatttcag cccagggttc ctaccctggg gaagaaggtt gtgtcgcaat
      241 tgttcaactc cgcaggagtt ggcgaactct tacagagttg atcgcggata attcgttgtt
      301 cccagggtag gaaaccctgg gctgaaattt gcgaacgcct gcggcgtatt gatttgtgca
      361 attggagtgg acgccactaa tcgcatcgag caccgacaaa ctcactgaat gaacgcactg
      421 cccataatca tcggagcgct ttgcgtaatt gccatctcct atcgctacta ctccgccttc
      481 atcgcggcga aagtgctcgc gctggatgat tcgcgcccgg tgccgtcgca gacgatgtac
      541 gacggtcaca actattaccc gactaacaag tgggtgctgt ttggacatca cttcgccgcg
      601 atctccggcg ccggaccgtt gatcggtcct gtgctcgccg ctcagtttgg ttttctgccg
      661 ggactgctgt ggttagtgat aggtgtctgt cttggcggcg cggttcatga cttcatgatc
      721 atggtgggca gtgttcgccg caaaggtaaa tcactggcgg agatggcgcg cacggagatc
      781 agtccgctgt cgggtctggt ggccggcgtt gcgattctgt ttattgtcgt tattgcgctt
      841 gcgggactcg ggctggttgt cgtcaatgct ttggcggagt cgccgtgggg aacgttcacc
      901 gtcggtttca cgattccgct ggcgttgttc atggggctgt acatgtatcg cttccgcaaa
      961 ggcaagattg tcgaagcgag catcatcggt attgtgggac tgctgtttgc agtttatctc
     1021 ggcgggcgca ttgcggagtc gcccgcggcc gaagctttca cgctttcgcg aaacagcctg
     1081 atcgtgatca tggcggccta tgggttcatt gcttcagtgc tgccggtttg gatgctgctc
     1141 tgcccgcgcg actacctgtc gtcgtatttg aaaatcggca cgatcgcctt tttgattctc
     1201 ggtgtaatca tcgtcgcgcc ggatttgaac atgccggctt tcacgccatt cacagccggc
     1261 ggcggaccag tcattcccgg caagctgtat ccattcgtct tcatcacgat tgcgtgcggc
     1321 gccatttccg gttttcacgc tctgatctct tccggcacga cgccgaagat gatcgcgaaa
     1381 gagaccgaca cgcggatgat tggctacggc agcatgttga tggaaggcat cgtcggcatt
     1441 gtcgcgctca tcgcggcggc gtcgctgttt cccggcgact acttcgccat caacacggcg
     1501 caaggcaccg aagcacagaa atcagcctac gttcgcatgg tcgacaccca ctcttcagaa
     1561 ggtttcaacc tgcaaccaca ggagatcgat cgactcgaac aggaatcagg cgagaaaaat
     1621 ctgcgcggtc gcacgggcgg cgccgtcacc ctcgcgattg gcatcgccaa aatctttgca
     1681 ggtattcccg gtctcggcgg tttcatgaag tactggtatc acttcgcgat catgttcgag
     1741 gcgctgttca tcctgacgac gatcgacacc gggacgcgtg ttgctcgttt tttggtcggt
     1801 gaatttggcg ggcggtttta tcgaaaactc gaagaaccaa actggatgcc cggctcggtg
     1861 atcacgtctg ccctggtagt ctccgcgtgg gcggcgttca tctggtcggg gtcgatctca
     1921 accatctggc cgatgtttgg cattgctaat caactgctcg cagccgtggc tttgtgtgtg
     1981 gccaccacga tcctggtcaa caccggtaag gcgaaatacg cgtgggtgac catcgtgccg
     2041 ctcagttttg tcagcactac gacgctggtg gcgggcttca aatccatcac cgacatcttc
     2101 tggccgttgg cgcagaagcc ggaaacctca acccagggcc tcattaacac cggcctgacc
     2161 gtgatcatca tgtttgccgc agtcgtgatc atgatcgact cgatcaggcg ctggctgggc
     2221 acgcgaaagg gtccggaggt ttcccaaatc ccagagcccc tctttgaaaa atccgtgtaa
     2281 ccgtggataa tacgcggttt cgtagcgtat ctcgccataa tctagtcatt ttcgaaagga
     2341 attctccgaa gtggaactat ccctattact gcggcgtgcc gcaatcgcca ttgctgcgat
     2401 gctgacgact ttggccttcg ggctgcccac ttttgcgcag cagccgcatc gacctggcgg
     2461 cgaggcgaac ctgcaacttc ctaaccttac ggaggtaaat ttcctcaaca acagcattag
     2521 tggtcacaac ctgctgctgt ttggcatcgc catctgcgtg cttggcctgc ttttcggtct
     2581 cgcgatgtac acgcaactgc gaaagatgcc ggtgcacagc gccatgcgcg agatctcgga
     2641 actgatctat gccacgtgta agacctatct gatcacgcag ggcaagttca ttctgattct
     2701 cgaggcgttc atcgcagtcg tgatcgttct ttacttcggt gtgctgtcag agatggagcc
     2761 gtttcgcgtg ctgatcatcc tgttcttcag tttggttggc atcgcgggca gttacggcgt
     2821 cgcgtggttt ggcattcgcg tcaatacctt tgccaactcg cgcaccgcgt tcgcgagtct
     2881 ccaggggaaa ccatttccga tttatgcgat tccactgaaa gcgggcatga gcatcggcat
     2941 gatgctgatc agcgtcgagt tgctgatcat gctgtgcatt ctgttgttca ttccgggaga
     3001 ttacgccgga ccgtgcttca tcggtttcgc aattggcgag tcgctgggag cggctgcgct
     3061 gcgtatcgcg ggcggcatct tcacgaagat cgccgacatc ggttccgacc tgatgaagat
     3121 cgtcttcaag attaaggaag acgacgcgcg caatccgggc gttattgctg actgcaccgg
     3181 cgataacgcg ggcgactcag ttggaccgag cgcggatggc ttcgaaacct acggcgtgac
     3241 cggcgtggcg ctgattacgt tcattctttt gggcgttccg aatcctatcg tgcaggtgca
     3301 gttgctcgta tggatcttcg tcatgcgcat catgatgatc gtggcgagcg cgctctccta
     3361 tttcgttaac gacgcgatcg cgaaggcacg tttcagaacg gccgattcga tgaacttcga
     3421 agcaccgctc acgtcgcttg tgtggatcac ttcgatcgtc tcggtcgtga tcacttacgt
     3481 tgtttcgtgg ttgaccattc cgacgttgag cggcaatcca acgctctggt ggaaactcgc
     3541 gaccatcatt tcgtgcggca cactcgcggg cgcgatcatt cctgaactgg taaaggtgtt
     3601 tacgtcgacg gagtcgcggc acgtgagtga agttgtcacg tcgtcgcgtg aaggcggtgc
     3661 gtcgctgaac atcctgtcgg gccttgttgc cggaaacttc tccggttact ggctcggcat
     3721 cagtatcgtg gcgttgatgg cgattgctta cttcttcact aatctcggtg cccccgctgg
     3781 tttggggctc ggggcgctaa tggtcgctga cgtggccgcg cctgtattcg gctttggcct
     3841 ggtggcgttt ggtttcctcg gcatggggcc agtgacaatc gccgtcgact cgtatggtcc
     3901 ggtgactgac aatgcgcaat cggtttacga gttgtcgttg atcgaacagg tgccgaacat
     3961 cgaagaggaa gtaaacaaag agtttggcat gactgtgaac ttcggccggg cgaaacactt
     4021 gctcgaagag aacgacggcg ccggcaacac gttcaaagcc acagcgaaac cggtgttgat
     4081 cggtacggcg gtcgtcggcg cgaccacgat gatcttcgcg atcatcatgg cgctgactga
     4141 cggactgcgc acgaacattg aaagtctgtc gttgctgcac gcgccttttg tactcggtct
     4201 catcacgggc ggcgcgatta tttactggtt caccggagcg tcaatccagg ccgttaccac
     4261 aggcgcttat cgcgcggtcg agtttatcaa ggcaaatatc cgcctggagg gtgtcgagag
     4321 ggcttcggtt gaagatagta agaaggtggt ccagatctgc acgcagtatg cgcagaaggg
     4381 tatgttcaac atcttcctga cggtgttctt ctcaacgttg gcgtttgcgt ttgtcgaacc
     4441 gttcttcttt attggctacc tgatgtcgat cgctgtgttt ggtttgtacc aggcgatctt
     4501 catggccaat gccggcggcg cgtgggacaa cgccaagaaa gttgtcgaag tcgaccttaa
     4561 gatgaagggc actccgctgc acgatgcgac cgtagtgggc gacacagtcg gcgatccgtt
     4621 caaagacact tcgtcagtgg cgatgaatcc ggtaatcaag tttacgacgc tgtttggact
     4681 gttggcggtg gaactcgctg ttagtttgac cgcgcgcaac aacggcgaca cgatgctgac
     4741 gcacattttg gcgctagcgt ttttcctggt gtcggtattc gtcctgtggc ggtcgttcca
     4801 cggcatgcgc attggaacga tggcgttgga gccgaggcca agcgagcgcg aggttgtcaa
     4861 atctgcggcg ggagactaac cacgaatgaa cacggatgta agagcagata cgttgttggg
     4921 cgccgcgctc gcgcccattg attggacccg ttcggttggg ctcgttgttg ttttcagtct
     4981 gttcattgcc gcggctgcgc agttcgcgat tcatattgga ccaataccga tcacagggca
     5041 gtcgttcgca gtgttgttga cgggcgcttt gctcggatcg cgtttgggtg cggcggcggt
     5101 gattgcttac ctgatcgaag gtgcggtggg cttgccattc tttgctccgg gtggagcgcc
     5161 cggcattctg cgcttttttg gcgccacagg cgggtatctc gttgcgtttc cggcggctgc
     5221 gttcgtgacc ggcgcgtttg ccgaacacgg atgggacaag aattatccgc aggcggtcgc
     5281 ggcaatggcg attggatcgg tgatcatttt gcttggtgga tgggcgtggt attcagttct
     5341 gactaacact ccgccaattg ctgcgttcca gattgcggtg tggccttatc ttgcgggcga
     5401 cctgattaag atttgtctcg cggccgcggt gcttccgacc ggttgggcac tgctgaagcg
     5461 caaagcttcc ggcaagtagc aaaagtatct gtgcctctgc gtctctgtgg tagtttaacc
     5521 actgagacgc ggagacacag agaagaacat gtacgtcttg aaggtaaagt cgcgccagcg
     5581 cgaagagctg gttgagttca ccgatctcgt tagagacaag ttgaaggagt ccggcgcgcg
     5641 cgaagggatc gtagttctct acgtacagca cacgaccgcc ggactcacca tcaacgaaaa
     5701 cgccgatccg gatgtgccgc gcgacatgtt gcatgccctc cgaactttga ttccacaaca
     5761 cggcatgggc tttcggcacg gcgaggaaaa ctcggacgcg catatcaaaa cgagtctggt
     5821 cggaacctcg gtcaccgttc ctttcaaaga cggcgagttg ttgctgggcc gctggcaggg
     5881 aatattcctt tgtgagtttg atggtggacg cgagcgccag gtgatcatga tggttcgttg
     5941 agcgacatgc tgaaagcctc catactcacg cttgtagcct gcatcgtaga tggcgcgtta
     6001 ggagttcagg tcgtcaggtt ttaattgatt aaggatttcc gcgaatttct cgatgtcgcg
     6061 tttggtgttt tccattgcgc gccggctgtc gagatatgcg gtccaggttg gcgtttgtgt
     6121 caacgctttg gtggtgtctt cgtcgagtac ctgggccata agcgcgatca ggtcactgac
     6181 gatgcgcgtg tgttcgacaa ggttttcgat gatcgaataa gcaagtcgcg cttgttcgaa
     6241 gtcgaggtcg cgcgctgagt tgttttcctc ggacattgtt ttagtcctga taccaactac
     6301 cacggattaa aggaagtgag tgggttgttg cgggtacgcc atcaacgatg atcgacgtac
     6361 ctggagcgag gtgttcatag cggacgtagc caagtccgat tgtggctgct aacttcggtg
     6421 agaacgttgt ggacgttacg cggccgatct ctttgtcgtc cgtcgatttg atcacggcgc
     6481 ccgcttcaat ttttgcatca gtttcaaaca ggaggcccgt caatttcttc gcgacgtgtc
     6541 cgcggtgcct gatgcgcacg atgatctcct gcccgacata acagcctttc gtgtagctga
     6601 cggcgtcgtc aagattcgtc tcagtgacga cgtttgtttc atccatgtcg cgcccatagc
     6661 gcgcaatccc cgcttcaatc cgtaaaatct ctgcaacccc ctcgctcacg ggagtagccc
     6721 aaaacgtctt cacaaaacct gaatcggcga tcacgtcaac cccatcttca cccgtgtgtg
     6781 tcgcacggat aatcgtcacg ccaccgtctt gccaaacacc gtcgcgcggc aaatcgctca
     6841 caccaaagat tttttcgacg agttcgccgg cgccgcgccc ctgtagtgaa atctggccgg
     6901 tttcctccgt cagatcccgg accctaaaat cgcccgcgag cgtaaaccgc tcaattattt
     6961 tcaatacttt gtcgcgcgtg attgcttcgg tatcgatcag aatgtcgcct gctcgtctga
     7021 tcacccgcac cgctgcgagc aaacgtccct gcacgtttgg aaacgccgcc ggcatccagc
     7081 gattctcctc gagagttttc atgtcgttcg tgatcagccc gttgagaaac atcactgctt
     7141 ccgaaccgct gacgctgatt cgcccgcgcg tcgagagatc gatcaagccc acgctgccgt
     7201 cgcgaacggc tgcatattcc gccatttcat tgctgtgtat cgctgtcgct tccgtcatgg
     7261 gtgcatccgc actgcttgtg ctactacctg gtaatccttc aactgttccg cctcacgccg
     7321 gtgcgaggcg agcacctgcg ggctcggatg atagatcggg accagcacgc gctcttgcca
     7381 cgaatgtatc ttcgcagccg actgtttcag atttaactcg tgatattgaa tgcgctttag
     7441 cgcctcgagt gcaaccgaac ccagcgttac gatcacgcat ggatcgatca gttcgatagt
     7501 tcgccgcagg aagtcggaac agttcgccat ctctcgtcgt gccggtttcc gattcgcgcc
     7561 tgtttcagtg cgtggattgc agagcgctgc gctggtgatg aagatctgcc ggcggctgag
     7621 acctatcgaa ccgaggaaac gatcgaagtt tgcgcctgac tgatcgcctg aaaacggcac
     7681 gcgggtgcgg tcagcgcctt tccggcccgg cgcttcgcca ataaacatga tgcgcgctcg
     7741 tggcgagccg ttgagtttgc tcaacacggc gctccgccca cacatcgccg gacaccgcgt
     7801 gcaaccggcg gcttcgtcaa ccagctcggc gaatagtttc tctttcgatt tctccatcag
     7861 cgaacttcgc ttcctgcggt cccagaaaag agctacgtca gaattgtcag gccgcgggtg
     7921 acgacgttg
//
LOCUS       DASTEY010000036         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_791923, whole genome shotgun sequence.
ACCESSION   DASTEY010000036 DASTEY010000000
VERSION     DASTEY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791923"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2088
                     /locus_tag="VFO72_00675"
     CDS             <1..>2088
                     /locus_tag="VFO72_00675"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET9477825.1"
                     /translation="KRLLWKLISAEQFERFLHTKYLGQKRFSLEGCETVIPVLDRLID
                     GAAARGVEHITFGMAHRGRLNVLANVIGNLCERIFAAFEGSVHPEFPADEGDVKYHQG
                     ATGERVLDDGRKVSLSLSPNPSHLEAVDPVVEGMVRATQDRELGQRNATREQVVDLAL
                     PVLLHGDAAFAGQGIVMETLNLAGLKGYRTGGTIHIIINNQIGFTTSPEAGRSTIYST
                     DVARMTQLPIFHINGDDPEAAYRVLQIALDYRQEFNKDVVLDLIGFRRLGHNETDEPS
                     YTQPLMYARVKAHPGVRTVYAERLIKEGVLTEEEVNNLIGERVRRYEDGLAKAKQKVA
                     DEPPQPPPPVEELDGSDVVPTGVDSQTIRNIAHKIAVVPEGFNVNPKMVGQLARRAKM
                     GDGSLPLDWGFAESVAFGSLVLDGTRVRLSGQDSGRGTFSQRHAVLYDTQTGKAWAPL
                     SELRSESNPGARFDVFDSSLSEQGVLGFEYGYSVEYDDALVLWEAQFGDFGNGAQTII
                     DQYIAASEDKWKQRSRLGMLLPHGYEGQGPEHSSARLERYLQLCAENNLQVCYPTTPA
                     QYFHLLRRQVRPGFERPLVIMTPKSLLRLPAAGSRLEALASGGFQPIIDDAEIADAAS
                     VRRVVFCSGKVYYDLMDARKQSGDLRAAIVRLEQFYPFPQRGVREVLAKYASATEFIW
                     AQEEPHNMGGWAFV"
BASE COUNT          446 a          551 c          659 g          432 t
ORIGIN      
        1 aaacgcctgc tctggaagct gatctcggca gaacagtttg aacgttttct gcacacgaaa
       61 tatctgggcc agaaacgctt ctcgctcgaa ggctgtgaaa cggtgattcc cgtgctcgat
      121 cgactgatcg acggcgcggc cgcacgcggt gtcgagcaca ttacgtttgg catggcccat
      181 cgtggacgtt tgaatgtgct cgcgaacgtg atcggaaacc tgtgtgaaag gatcttcgcc
      241 gcgttcgaag gctcggtgca tccggaattt cccgcggacg aaggcgacgt gaagtatcac
      301 cagggcgcga ctggtgagcg cgttctggac gacggtcgca aagtcagcct gtcgctctcg
      361 ccgaatccga gccatctcga ggcagtcgat ccggttgtcg aaggaatggt ccgtgcgaca
      421 caggatcgag aactgggcca acgcaatgcc acgcgcgaac aagtggtcga tctcgcactc
      481 cccgtcctgc ttcacggcga cgctgctttt gcgggtcagg gcatcgtgat ggaaacattg
      541 aacctcgccg gtttgaaagg ctatcgcacc ggcggcacga tccacatcat catcaacaac
      601 cagatcggct tcacaacttc acccgaagcc ggccgctcga cgatctattc aactgacgtg
      661 gcgcgcatga ctcagttgcc gatcttccac atcaatggcg atgatccgga agctgcatac
      721 cgcgtcctgc aaatcgcact tgattaccgg caggagttca acaaagacgt cgtgctcgat
      781 ttgattggtt ttcgtcgtct cggtcacaac gagactgatg agccaagcta cacgcagcca
      841 ctgatgtacg cgcgcgtcaa agcacatccc ggcgtgcgca cggtttacgc cgagcggttg
      901 atcaaggaag gcgtgttaac cgaagaagaa gttaataatc tgatcggtga acgcgtgcgc
      961 cgctacgaag acgggctggc gaaggcgaag caaaaggttg cagacgaacc gccacaacct
     1021 cctccgccgg tcgaggagct cgacggttcg gacgtagtcc cgaccggcgt agattctcag
     1081 acaatcagaa acatcgctca caagatcgca gttgttccag aagggttcaa cgtgaatccg
     1141 aagatggtgg gccaactggc gcggcgcgcg aagatgggcg acggatcgtt gccgcttgat
     1201 tggggttttg cggagtcggt cgcgttcggt tcactcgtgc ttgacggcac acgcgtgcgt
     1261 ttgagcggac aggattcagg tcgcggaacg ttcagccaac gtcacgctgt gctttacgac
     1321 acgcaaaccg gaaaggcgtg ggcgccgctg tcggagttgc ggtcggagtc gaatcccggt
     1381 gcgcggtttg acgtgtttga cagttcactg tcggaacagg gcgtgctcgg gtttgagtac
     1441 ggctattcag tcgaatatga cgacgcactc gtattgtggg aagcgcagtt tggtgatttc
     1501 ggcaatggcg cgcaaacaat catcgatcaa tacatcgctg cgtcggaaga caagtggaaa
     1561 cagcgatcgc ggctcgggat gttgctgccg catggttatg aaggacaagg accggaacat
     1621 tcgagtgcgc gactcgagcg ttaccttcag ttgtgtgccg aaaacaatct tcaggtctgt
     1681 tatccgacga cgcccgcgca gtatttccac ttgctgcggc gacaggtgcg gcctggattc
     1741 gagcggccgt tggtgataat gacgccgaag agtctgctgc gtttgccggc ggcgggttcg
     1801 cgtcttgaag ctctcgcgag cggcgggttt cagccgatca tcgacgacgc ggagattgcg
     1861 gatgcggcaa gcgtgcggcg cgtcgtgttt tgtagcggca aggtttatta cgacctgatg
     1921 gacgcgcgca agcaaagcgg agatctgcgc gcggcgatcg tgcgtttgga acagttctat
     1981 ccatttccgc agcggggcgt gcgcgaagta ttggcgaaat atgcgagcgc gacggagttt
     2041 atatgggcgc aggaagagcc acataacatg ggtgggtggg cgttcgtg
//
LOCUS       DASTEY010000037         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_346852, whole genome shotgun sequence.
ACCESSION   DASTEY010000037 DASTEY010000000
VERSION     DASTEY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346852"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFO72_00680"
     CDS             complement(<1..936)
                     /locus_tag="VFO72_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9477826.1"
                     /translation="MSHSNLTYEFGPYRLNVSNRLLTRAGETVSLTPKAADILALLVM
                     NAGQLVEKNDLLKEVWPDTFVEESNLTQNIFALRRALGDERAGPKYIETVTRRGYRFI
                     ASVRVYDADAQNLSVAESVSASESDEADAASQRLVIAVLPFVNATGDGGVEYLAEGIT
                     DNIINNLSRVSKLRVMSRSAVFRRKEEVLDPLLIGRELGAKAVLVGKISARPVGIAIG
                     FELVDVATGWQLWGETFECELKDLLEIQDAITRQLLVNLKLTLTGEEEKRITARYTEN
                     AAAYQAYLEGRYHWSRYTRKGIEKAIVHFRQAIALD"
     gene            1321..2229
                     /locus_tag="VFO72_00685"
     CDS             1321..2229
                     /locus_tag="VFO72_00685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9477827.1"
                     /translation="MSEATSQLKERVRAFWQQHPCGTKFADAEPGSTKFYELVEEHRY
                     KKEWHIPAAAGFCETKDLAVLEIGCGLGTDGAQFAKAGAHYTGIDLTEAAVDLARRRF
                     QLFNLRGSFRVADAERLDFPDNSFDLVYSHGVLHHTPDTPAAVREIHRVLRPGGRAVV
                     MLYHRDSYNYRINISVLRRAGVHLLRWNTGVKLVRALTGESEESLRGHARQLRSNHEY
                     LSSNEFLSRNTDGAGNPLARVYSRKEARELFKDFATVELRPYFLNKRWLPIVGPVLPR
                     SLEAKLASRWGWHLWIYATKRTTKER"
     gene            2226..3185
                     /locus_tag="VFO72_00690"
     CDS             2226..3185
                     /locus_tag="VFO72_00690"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9477828.1"
                     /translation="MKNLLVTGGAGFIGSHLVDRLLQSDIDRLTVVDDFNNFYEPEIK
                     RANTIEHLKDQRYTLAAIDIRDRAALETLFNQHRFDCIVHLAARAGVRPSLKEPQLYT
                     ETNVNGTVNLLELARYHGVEQFVFGSSSSVYGINAKVPFSEDDPIRQPISPYAATKAA
                     GELLCHTYSHLYGLRCVCLRFFTVYGPRQRPDLAIHKFAKLISQGKPIPVFGDGTTRR
                     DYTYVDDIIDGVVAAIDYDKSDYEVFNLGESRTVELRELISLLEKELDAHATIDRQPP
                     QPGDVPQTFADITKARALLGYDPQTQIEEGLHRFVEWFRGKLV"
     gene            3197..>3436
                     /locus_tag="VFO72_00695"
     CDS             3197..>3436
                     /locus_tag="VFO72_00695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9477829.1"
                     /translation="MRVLDDQEILKQHAPATHAADAPEVSVFLPVYNEEPNLRPLHEK
                     LDAALKSLGRSAEIVYVDDGSSDGSLKILREIANED"
BASE COUNT          782 a         1048 c          864 g          742 t
ORIGIN      
        1 gtccagggcg atggcctgcc gaaaatgaac gatcgctttt tcaatcccct tccgcgtgta
       61 tcgactccaa tgataacgac cctccaaata cgcctgatag gccgccgcat tctccgtgta
      121 acgcgcggtg atccgtttct cttcctcgcc cgttaacgtc agcttcaggt tcactaataa
      181 ctgccgcgta atcgcgtcct gaatctccag cagatccttg agttcgcact cgaaagtctc
      241 gccccacaac tgccaacccg ttgcaacgtc taccaactca aacccaatcg caatccccac
      301 cggccgcgcg ctgatcttcc cgaccagcac ggccttcgca cccaactccc tcccaatgag
      361 cagtggatcc agtacctcct ccttgcggcg aaacaccgca ctacgagaca tcacgcgcag
      421 cttggaaaca cgagagagat tgttgatgat gttgtcggta attccctccg ccaggtattc
      481 cacgccccca tcacccgtcg cgttgacaaa cggcagcacg gcgatcacca aacgttgcga
      541 ggccgcatcc gcctcgtcag attcggacgc actgacggat tcagcgacgc tcaggttttg
      601 agcatcggcg tcataaactc tgacgctggc gataaaacgg tagccgcgtc ttgtcacggt
      661 ctcaatgtac tttggaccgg cacgttcatc gcccaacgct cgtctcagcg cgaagatgtt
      721 ttgcgtaagg ttcgactctt cgacaaacgt gtcgggccaa acctccttca gcagatcatt
      781 cttctccacc agctgacccg cattcatcac caacaaggcg agaatgtcag ccgctttggg
      841 tgtgagtgag acggtttcac ccgcgcgcgt caagagacgg tttgatacat tgagccgata
      901 tggaccaaat tcgtaagtga ggttgctgtg agacataaac ttcgtgagaa caattaaata
      961 actttttgat ttagttctaa ggatttccgc ctgcagagtg agtatagttc gcgccgtcgt
     1021 caaacgccgc tgccatttac aggctgcgtg attctaaaag caagtctcac gaatggcaag
     1081 atccgagagc gtctcgtttg tgcgtttggt ctggcccagt tgagagaggg ggcaatgtca
     1141 actggtggac aggtgggtgt ccttagccgg acgtgggaac ctcaccccag cgaccttcgc
     1201 tgtgcttgtg cctcatggcg gcttcgccca gcaagactgg ccatgcggca caagacagcg
     1261 aagtaaaaac gatctttgtc ggcgcacttc tcaccctgtt atgcttctgc atctgttgcg
     1321 atgtctgaag caacctccca acttaaagaa agggtgcgcg cgttttggca gcagcatccc
     1381 tgtgggacca agtttgccga cgccgaaccg ggttccacta agttctatga actcgtcgaa
     1441 gagcatcgct acaaaaagga atggcacatt cccgccgccg ccggcttctg cgaaacaaaa
     1501 gatctcgcgg tgctcgaaat cggttgcgga ctcggcactg acggcgcaca gttcgccaaa
     1561 gccggcgccc attataccgg catcgatcta acagaagccg ccgtcgacct cgcacggcgt
     1621 cgtttccagc tcttcaatct acgcggatcg tttcgcgtgg cagacgccga acggttggat
     1681 tttccggata actcgttcga tctggtctat tcgcacggcg tccttcatca cacgcccgat
     1741 acacctgccg cggtacgcga aattcatcgt gtgcttcggc caggtggccg tgccgtcgtg
     1801 atgctctatc accgcgactc gtataactac cgcatcaata tctctgtgct cagacgcgcc
     1861 ggcgtgcacc tgctgcgatg gaacacaggc gtgaaactcg ttcgcgctct caccggcgaa
     1921 agcgaagaat cgctccgcgg gcacgcgcga cagttgcgat caaatcacga atatctgagc
     1981 tcgaacgaat tcttaagccg gaacaccgac ggcgccggta atccgctagc gcgagtctat
     2041 tctcgtaaag aggcacgcga actctttaaa gactttgcca cagtcgaact ccgtccatac
     2101 ttcttgaaca agcgctggtt gccaatagta ggtcccgtgc ttccgcgctc actggaagca
     2161 aaactcgcat cgcgctgggg ctggcatttg tggatttatg ctacaaagag gactacaaag
     2221 gaacgatgaa aaatcttctc gtaaccgggg gcgcagggtt catcggctct catctcgtcg
     2281 atcgtttgct tcagagcgac atcgatcgcc tcactgtcgt tgacgacttc aacaactttt
     2341 acgaacccga aatcaaacgc gcaaacacca tcgaacacct taaggaccaa cgttacacgc
     2401 ttgcagcgat cgacattcgc gatcgcgctg cactggagac actcttcaac cagcaccgct
     2461 ttgattgcat cgtgcatctc gccgcgcgcg ccggcgtgcg cccttcgctg aaagagccac
     2521 agctctacac cgaaaccaac gtcaacggca cagtcaacct gctggaactc gcgcgttacc
     2581 acggagtcga acaattcgtc tttggttcgt cttcatcggt ctacggcatc aacgctaaag
     2641 tccccttcag cgaagacgat ccgattcgcc aacccatctc gccttacgcc gccacgaaag
     2701 cggcaggcga acttctttgc catacgtact cacatctcta cggtcttcgt tgcgtgtgtc
     2761 tacgcttttt caccgtctat ggaccacgcc agcgtccgga tctcgcgatt cacaagtttg
     2821 cgaaactgat tagccagggc aaaccgattc ccgtctttgg cgacggcacc actcgtcgcg
     2881 actacactta cgtcgacgac atcatcgacg gcgtcgtcgc tgcgattgac tacgacaaga
     2941 gtgattacga agtgttcaat ctgggcgagt cgcgcacagt tgagttaaga gaactgattt
     3001 ccctgctgga aaaggaactc gacgcgcacg cgacaatcga ccgccaacct ccgcaaccgg
     3061 gcgacgtgcc gcaaacattc gccgacatca ccaaggcgcg cgcgctgctc ggctatgacc
     3121 cgcaaacgca gattgaagag ggcctgcacc ggtttgttga gtggttccgg gggaaattgg
     3181 tctagaatgc tcaacgatga gagtcctcga cgaccaggaa attctgaaac agcacgcgcc
     3241 ggcaacccat gccgcggatg cgccggaagt ttccgtcttt cttcccgtct acaacgaaga
     3301 accaaacctg cgtccgctgc atgaaaagct cgacgcggcg ctgaagtctc tgggtcgctc
     3361 cgccgagatc gtttacgtag acgatggttc ctccgacggc agcctgaaga tcctgcgcga
     3421 aattgcgaac gaggat
//
LOCUS       DASTEY010000038         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1566345, whole genome shotgun sequence.
ACCESSION   DASTEY010000038 DASTEY010000000
VERSION     DASTEY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566345"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VFO72_00700"
     CDS             <1..1322
                     /locus_tag="VFO72_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9477830.1"
                     /translation="RTAAALVVGEVIAVGIFLTPAGMAKQLGSPLWLLVVWLAMGAMA
                     LCGALCYGELSARYPEAGGGYVYLREAYGPAVAFMYGWMAFLVMDPGLTAALAVGLAT
                     YAGYVVNLTPPATKLLAIAAIVAIALVNIRGVRLGAWFVRVLMFLKLGLLAFVILWGV
                     GFQLGDWSNFTPFIARHPNSPPLIGALAGGLVGAFFSFAGWWDLGKLAGEVKRPARTL
                     PKALILGVITVTVVYILTSLVFVYLVPLDQVTSGETFAAQAGEVLFGRAGGTVFSLIV
                     VVAVLGSLAAVVMSAPRVYFAMARDGLFVPAVASIHPRFETPAFAIALQAALASVLVL
                     AGTFNDIISYFIFVVVVFIGLTVVALFRLRRRAPHDAGYLTPGYPVTPIIFLVLIVVL
                     LVLLGGNNPKQALLGVAVVSLGLPFYLLVFRHRRSIEKEGRTSYDLD"
     gene            1309..1590
                     /locus_tag="VFO72_00705"
     CDS             1309..1590
                     /locus_tag="VFO72_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477831.1"
                     /translation="MTWIKTVAVTEDDEVRKAVEAQRDLYPIEYAMPVHPTADGETAG
                     IVASHSLIPKALFHAFSTFGSLMTPDLPLTRRQHEMITTVVSVTNRCHY"
     gene            1621..1875
                     /locus_tag="VFO72_00710"
     CDS             1621..1875
                     /locus_tag="VFO72_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477832.1"
                     /translation="MTLDQELVAALEEDYASAPISEQERVMLDYVVQITRDATRISPA
                     DHDRLRAVGFDDKGILQITLIASWFNYINRVADALGVGRG"
     gene            1879..4032
                     /locus_tag="VFO72_00715"
     CDS             1879..4032
                     /locus_tag="VFO72_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9477833.1"
                     /translation="MKYGAVVLVAIMLGSVVFAQQDDDVVRVKSNLVNIDVIVKDKKG
                     KYIADLKAEDFTVVENGVAQKIEFFDAPLSRSETPKGETKSAPAATSEATTTTSAARN
                     YVALVVDSQTTDITNLKQVREGTLKYIREQITENDIVALLTVTNGLQMLQPFTQDKTK
                     LIAALENAGVNSASKSFEQKDLAGNISSRQQQLLEGASTPATAVSSPAAGSEAAKLMI
                     AQRVLAQFVQLRTALGLQQSRPVLAALAAICEGLKPIPGKKTLVLFSQGFVTPAVLDW
                     QVQSTIDIANRANVAIYIIDSAGLRGAAPASGSLVPSSPLAGVSGLVNQEQRIRAVGG
                     ETVFDNVRHEGKSREYDILYRISGDTGGRFLKGNNDIGQGLERINEEIQARYTIAYRS
                     TNQNFDGTFRKVKIEVRRPDAQIVSRSGYYAIPPEEIVLLSPADKKLLSGFAAAQSNP
                     ELPVFVGLSQFRSREGLYTVPLAIELPPAAVKFERKGDQRSMQLEVLGVLKATPDRIL
                     SRLGGNFDVNLTDSDYQSIVNNNIFYRQDMVLAPGEYTLDVIVRDQQSGKTAAKREQL
                     SLPEPDSEFAATPVVLSRFVEQAKLPELGSDEPQDVLAYGRAQIRPSPGRQFQASDNL
                     IMFLSVYNAANSPDTSKPLVRVSVRLMKEGTPVTKFFDYVLSDIQPEPVPHLTFAEYI
                     RLAGLPAGNYTAAIEIKDMVTRKFVKQDAPFTIVP"
     gene            4268..>4902
                     /locus_tag="VFO72_00720"
     CDS             4268..>4902
                     /locus_tag="VFO72_00720"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9477834.1"
                     /translation="MLISELDTPALVVDLDVMQQNLSRMAEYCRAHNLRLRPHTKTHK
                     IPELARMQIESGASGITVAKLSEAEVMLDAGLNDLLIAYPIVGPTKATRLATLAERAT
                     LTVSLDSAEAARGISTAMSERGTSAGILVELDAGFGRCGVPNESEALKLAQTVFSLPG
                     LTFEGLMFFPGHFGVAPDERTVLRKQVNELLDRCLETFARAAMPLRTISGGS"
BASE COUNT          977 a         1357 c         1490 g         1078 t
ORIGIN      
        1 tacgcacggc ggcggcgctt gtcgtcggcg aggtgatcgc ggtcgggatt tttttgacgc
       61 cggccgggat ggctaaacag ctcggctcgc cgctgtggct cctcgtcgtg tggctggcca
      121 tgggcgcgat ggcgctctgc ggtgcgctct gttatggcga actctccgca cgatatcccg
      181 aggccggcgg cggttacgtt tatctgcgcg aggcgtatgg cccagcggtc gctttcatgt
      241 acggctggat ggcgtttctc gttatggacc caggactaac agccgccctc gcggtcggtc
      301 tcgcgaccta tgccggctat gtcgttaacc tgacaccgcc ggccacgaaa ctactggcaa
      361 tcgccgcgat cgtcgcaatc gcactcgtta atattcgtgg cgtccggctc ggcgcgtggt
      421 tcgtacgcgt cttgatgttt ctaaaactgg gtctgttggc atttgtgatc ctgtggggcg
      481 ttggctttca actcggcgac tggtccaact ttacgccctt cattgcgcga catccaaatt
      541 cgccgccgct catcggcgcg ctcgccggcg gattggtcgg cgcattcttt tccttcgcgg
      601 gttggtggga cctcgggaaa ctcgccgggg aagtcaagcg tccggcgcga acgttgccga
      661 aagcactgat actcggcgtg atcacggtga cggttgttta catcctgacg agcctggttt
      721 ttgtctatct cgttccgctc gatcaggtca cgtcaggcga gacatttgcg gcgcaggcgg
      781 gtgaagtgtt gttcgggcgc gctggcggaa cggtgttctc gttgatcgtc gtcgtggctg
      841 tgctgggaag tttggcggcg gtcgtgatgt cggcgccgcg cgtctacttc gcgatggcgc
      901 gtgacgggtt gttcgtgccg gcggtggcgt cgattcatcc acgcttcgaa acgccggcat
      961 ttgccattgc gctgcaagcc gcgctggctt ctgtgctggt gttggctgga acgttcaacg
     1021 acatcatttc ctactttatc tttgtagtgg tagtgttcat cggtctgacg gttgttgccc
     1081 tgtttcgact acgccgtcgc gcgccacacg acgccggcta tttaacgccg ggttatccgg
     1141 tgacgccgat tatcttcctc gtattgattg ttgtgctgct cgtgttgctt ggcgggaaca
     1201 atccgaagca ggccctgctc ggtgtcgcgg tggtctcatt gggcctgccg ttttatctgc
     1261 tggtgtttcg tcatagaaga tctatagaaa aagagggacg aacaagttat gacctggatt
     1321 aaaacggttg cggttacgga agacgatgaa gtgcgcaagg cggtcgaggc gcaacgcgac
     1381 ctttacccaa tcgaatatgc catgccggtc catcctacgg ctgacggcga aacggcaggc
     1441 atcgtcgcgt cgcacagctt gattccgaaa gcgctctttc acgcgttctc aactttcggc
     1501 tcgctgatga cgccggatct gcctttgacg cgtcgccagc acgagatgat cacgacggtc
     1561 gtttcggtga ccaaccggtg tcattattga atcgaatcgc acgcagagtt tctgcgtcga
     1621 gtgacactgg atcaggagct ggtcgcggca ctggaggagg actacgcttc tgcgccgatc
     1681 agcgagcagg agagagtcat gctcgattac gtcgtgcaga ttactcgtga tgcgacgcgt
     1741 atttcacccg cggatcacga tcgtttgcgc gcggtcggct ttgacgacaa gggcatcctt
     1801 cagatcactt tgattgcctc atggttcaac tacatcaatc gcgtggctga cgcacttggt
     1861 gtcggcagag ggtaaaaaat gaagtacggt gcagttgttt tggtcgcgat tatgctcggc
     1921 tccgtcgtgt ttgctcagca ggacgacgac gtcgtacgag ttaaatcgaa tcttgtcaac
     1981 atcgacgtga tcgtaaaaga caagaagggc aagtacatcg cggatctgaa agcagaagat
     2041 ttcactgtgg tggaaaacgg agtggcgcag aagatcgagt tcttcgatgc gccgttgtcg
     2101 cgaagcgaaa ccccgaaagg cgagacgaag agcgcgcccg ccgccacgtc tgaagcaaca
     2161 acaacaacct cggccgcgcg aaattacgtc gcattggtgg ttgattcaca aaccaccgac
     2221 atcactaacc tcaaacaggt tcgcgaaggc accctcaaat acattcgcga gcagattacg
     2281 gaaaacgaca ttgtcgcgct gctgaccgtg acgaatggct tgcagatgct gcaaccgttc
     2341 actcaagaca agacgaagct gatcgctgcg ctcgagaacg ccggcgtcaa ctccgcttcg
     2401 aagagcttcg aacagaaaga tctcgccggc aacatcagtt cgcgccagca acagttgttg
     2461 gagggtgcat cgacaccggc gacggcagtc tcgtcacctg ccgccggtag cgaagcggcg
     2521 aagttaatga tcgcgcagcg cgtgttggcc cagtttgtgc agttgcgcac tgcgttgggc
     2581 ctgcaacagt cgcgtccggt gctggcggca ctggccgcga tctgtgaggg gctgaagccg
     2641 ataccaggta agaagacgct ggtcctgttt tcgcagggat ttgtcacgcc cgcggtgctc
     2701 gattggcagg tgcaaagcac gatcgacatc gcgaaccgcg cgaacgtcgc gatctacatc
     2761 atcgattccg ctgggctgcg cggcgcggcg ccggcgtccg gttcgctggt gccgtcttct
     2821 ccgctcgctg gtgtttccgg tctcgtcaat caggagcaac gaattcgcgc ggttggcggc
     2881 gagactgtgt tcgacaacgt gagacacgaa ggaaagagtc gcgagtacga tattctctat
     2941 cgcatctcag gcgacactgg cgggagattt ctcaaaggca acaacgacat cggccaggga
     3001 ctcgaacgaa tcaacgagga gatacaggcg cgttacacga tcgcttatcg cagcacgaac
     3061 cagaacttcg acggtacatt tcgcaaagtg aagatcgaag ttcgccgtcc tgatgcgcag
     3121 atcgtttcgc gctcgggtta ctatgcgatt ccgcccgaag agatcgtgct gttgtcgccg
     3181 gctgataaaa aactgctgag tggtttcgcg gctgcgcaaa gcaatccgga gttgcccgta
     3241 tttgtaggct tgagccagtt caggtcgcgc gagggtttgt acacggtgcc gctcgcgatc
     3301 gagttgccgc cggcggcggt gaagtttgag cgtaagggtg accagcgttc catgcaactg
     3361 gaagtgctcg gagtgttgaa agcaactccg gacagaattc tttcgcgact tggaggaaat
     3421 ttcgacgtca acctgacgga cagcgattat cagtcgatcg tcaacaacaa tattttctat
     3481 cgtcaggaca tggtgctcgc gccgggcgag tacactttgg acgtgattgt cagggatcag
     3541 caatcgggta agactgccgc gaagcgggag cagctttcgc tgccggagcc ggactcagaa
     3601 ttcgcggcga cgccggttgt tctgagtcgt tttgtggagc aggcaaagct gcccgagctt
     3661 ggttcggacg agccgcagga tgtgcttgcg tacgggcggg cgcagattcg tccgtcgccg
     3721 ggacggcagt ttcaagcgag tgacaacctg atcatgtttc tgtctgtcta caatgcggcg
     3781 aatagtccgg acacaagtaa gccgttggtc cgcgtatcgg tgcgtttgat gaaggaaggg
     3841 acgccggtta cgaagttttt tgactacgtg ttgagtgaca tccagccaga gccagtgccg
     3901 cacctgacgt ttgcggaata cattcgactc gccggactgc ccgcgggcaa ctacaccgct
     3961 gcgattgaga tcaaggacat ggttacgcgc aagttcgtaa agcaggacgc gccgttcacg
     4021 atcgtgccgt gacgcggtga ggcatgcgtt gccgcgcgcc gcaggcgacg ataggaccaa
     4081 gtgaaggcgc ccgcagcgaa gtcaagcgcc gaaggcgaga gcaggtagcg aagcggagcg
     4141 ctgcccctgg atcgccatca caacaacatt gcaggcctga aaggccggca gataccggcg
     4201 ctgccacgtg tctatcgccc tttcagtgct ttccggtttc ttgtatttgc gtctcgggac
     4261 gtaacccatg ctcatctctg aactcgacac gcctgcgctt gttgtcgatc tcgacgtcat
     4321 gcagcagaac ttgtcgcgca tggccgaata ctgccgcgcg cataaccttc gcctgcggcc
     4381 acacacgaag actcataaaa ttcccgaact cgcacgcatg cagatcgaga gcggcgccag
     4441 cgggattacc gtggcaaaac tcagcgaggc ggaagtcatg ctcgatgccg gcctcaacga
     4501 cctcttgatt gcatatccga tcgttggacc aacaaaggcc acacggcttg ccacgctcgc
     4561 tgaacgcgcg acgctcactg tgtcactcga ttcagcagaa gcagcgcgtg gcatttcaac
     4621 agcgatgtcc gaacgcggaa ccagcgccgg cattctcgtc gaactagacg ccggcttcgg
     4681 tcgttgcggc gtcccaaacg agtcagaagc gctgaagttg gcccagaccg ttttttcact
     4741 gccaggcttg accttcgagg gattgatgtt cttcccggga cacttcggcg tcgctcccga
     4801 cgaacgaacc gtcttgcgca aacaagtcaa cgaactcctg gaccgctgcc tggaaacatt
     4861 cgcgcgtgct gccatgcccc ttcgaaccat aagcggcggt tc
//
LOCUS       DASTEY010000039         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_412861, whole genome shotgun sequence.
ACCESSION   DASTEY010000039 DASTEY010000000
VERSION     DASTEY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412861"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VFO72_00725"
     CDS             <1..969
                     /locus_tag="VFO72_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HET9477835.1"
                     /translation="IASLRPDWFGFVSPELIRIASVQIPFLLLTLIGLNILLALGKLR
                     EFNLLDLVGQSFVLVNAILILIVFGLGLDALVKLNTITSVLISLVVVGLLVAGGKKLV
                     DSKWRADAALLRRMIIYGLKFHVSVLAGAIIFRADLLVVNHFRGPAEAGVYSVATQFG
                     LLLMLLPGVIATLLFPRVTAERDAHGETTWLVTRQTALIMLVCCLGAVPVSFLLPLVY
                     GAAFSEATALLLILLPGVYLIGLESVLVQHFNARGLPHTIPIYWLVTLVVNLVLVFAL
                     VPQFGAYGAAIASTVSYALIFGLVAFYFQATTRRSVMTAARGSLRG"
     gene            966..>2030
                     /locus_tag="VFO72_00730"
     CDS             966..>2030
                     /locus_tag="VFO72_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9477836.1"
                     /translation="MKVVLLALSGDSACCRAKLVELYPQATIETVSRAEFETGSLMRR
                     LSSLRTRRPDVFAIATERLAWQRGQNLFMTFGALSGARESIVIDSHGGFVRRTRGELL
                     ALAPLRLATEAFNGAADLAQSRRELRLLEQEVARTNRPAHVRTREAGPPRAVYLRATP
                     GPGTQAGGAASHIKGVVDALRALGVELRIISNDEIAGIDASDERFTVVSPETVGSTRA
                     LFDMHNNLVFTRAAVPLIQQANPDFIYQRYARFSWVGVAAALHTARPLFLEYNGSEVW
                     VGKHWDRVGSLGLLERYERLNLEAAARIFVVSEVERRNLEARGVAAEKIVLNPNAVDV
                     EAFRPGVGGAALRRELGIKNT"
BASE COUNT          341 a          516 c          640 g          533 t
ORIGIN      
        1 attgcgtctc tgcgcccgga ttggtttggc ttcgtttccc cggaactgat ccgaatcgca
       61 tccgttcaaa tccctttcct gttactcacg ctcatcggcc ttaacattct tctcgcgctc
      121 ggaaaactac gagagttcaa cctgctggac cttgtgggtc agtcgtttgt tttggtcaac
      181 gcgatattga ttctgatcgt ctttggcctg ggtctggacg cgctggtgaa gttgaacact
      241 atcacgagcg ttttgatcag tctcgtggtt gtcgggctgc tcgttgccgg tgggaaaaag
      301 ctcgttgatt ccaaatggcg tgcggatgct gcgctgctgc ggcgcatgat tatttacggg
      361 ctcaagtttc acgtctctgt tttggcgggt gcgatcatct ttcgcgcgga cttactggtc
      421 gtgaaccact ttcgtggacc agcagaagcg ggcgtctaca gcgtggcgac gcagtttggt
      481 ctgttgctga tgctgttacc cggcgtgatc gcgacgctgt tgtttccgcg tgtgacggct
      541 gagcgtgacg cgcatggcga gaccacctgg cttgtcacca ggcaaacggc gctcatcatg
      601 cttgtttgtt gtcttggcgc ggtgccggtc agtttcttgc tgccgttggt ttatggagcc
      661 gctttttccg aagcgaccgc cttgttactg atcctgctgc ccggtgttta tctcattgga
      721 ctagagtcgg tgctggtcca acatttcaat gcccgcggct tgcctcacac gatcccgatt
      781 tactggcttg tgactctggt tgtgaatctg gttcttgtgt tcgcacttgt gccgcagttc
      841 ggtgcgtacg gcgctgctat tgcgtcgacg gtaagttacg cattgatttt tggtctggtc
      901 gctttttact tccaagccac tacacgccga tccgtgatga cggcagcgcg cgggagtttg
      961 cgtggatgaa agtcgtgctg cttgcactca gcggcgactc cgcgtgttgt cgcgccaaac
     1021 tcgtcgaact ttatccgcag gcaacaatcg aaacggtttc gcgtgcggaa ttcgaaaccg
     1081 gtagtttgat gagacgattg tcgtctttgc gcacgcgtcg gccggacgtg tttgcgattg
     1141 cgaccgagcg gcttgcatgg caacgtgggc aaaatctttt catgactttt ggcgcgttgt
     1201 cgggcgcgcg tgaatcgatc gtgatcgaca gtcatggcgg gttcgtgcga aggacgcgcg
     1261 gcgagttgct ggcgcttgcg ccgttgagac tggctacgga agcattcaat ggcgccgcag
     1321 atctcgcgca gtcgcgtcgt gagttgcggc ttttggaaca ggaggttgcg aggacgaacc
     1381 ggcctgctca tgtgcgaacg cgtgaggcgg gtcctccgag agctgtttac ctgcgtgcga
     1441 cgcctggtcc aggcacgcag gcaggcggcg cggccagtca tatcaagggc gtcgtcgacg
     1501 cgcttcgcgc gcttggagtc gaactacgga tcatcagtaa cgacgaaatc gctggtattg
     1561 atgcttcaga cgagcgattc accgtcgttt caccggaaac ggtcggcagc acacgggcgc
     1621 tgtttgatat gcacaacaac ctggttttca cgcgcgccgc cgtgccgttg attcaacaag
     1681 ccaatccgga ctttatttat caacgttatg cgcgcttcag ttgggtcggt gtagctgcgg
     1741 cgttgcacac agcgcgtccg ctgtttcttg aatacaacgg atcggaagtc tgggtcggga
     1801 agcactggga ccgtgtgggc agtcttggtc tattggagcg atacgagcga ttgaaccttg
     1861 aggcggcggc gcgtattttt gtcgtgtcgg aagtggagcg gagaaacctc gaggcgcgag
     1921 gtgttgcggc tgagaagatc gttttgaatc cgaatgcggt tgatgtggag gcctttcggc
     1981 cgggtgttgg gggcgccgct ttgcggcgcg agttagggat taaaaacacg
//
LOCUS       DASTEY010000040         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_758960, whole genome shotgun sequence.
ACCESSION   DASTEY010000040 DASTEY010000000
VERSION     DASTEY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758960"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VFO72_00735"
     CDS             complement(<1..1626)
                     /locus_tag="VFO72_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9477837.1"
                     /translation="MIESRKEFVEDWQEADTEELSEEPETSAANTSRRSFFWLQSLAF
                     VFGLALLIYVINRVGVQPLFDALLRIGIGFFVILGLSGLRHFLRTIAMRAAVPAEHRQ
                     ITFRQAFAARLGGEAISFLTFTGPLLGEATKVALLRKRVPLTYGVPALVVDNLLYNLS
                     VVFFILSGACVMLMVYPLPQEVNVVLTGIALVAALGIVFVAIAAKRRVMLLTWIIDRL
                     AQLRLSPKVILKRRDHIYHIELKVYDFYKHHPGAFFVMIACNLLAHATSVVEVFLALR
                     MLGFDPQWAQAYIIESLTKVINFVFSFVPGTIGVYEGGTEVILQKGLGFTPAAGLALA
                     LVRKAAIVAWTSVGLLILTWRTLPNIWQRILNRSPRLQRLMDSLVFSNIAHRPARTAV
                     SVLGTAVGVLLIVFTVGLAHGVLHERGRRESNIGAEIMIRASGSVGLGGGSQFRLPVA
                     HAAELAAIPGVRAATPVGQTLDKSDSGFGQRLIDGINYDEYSALARITIREGRQLESG
                     DEAIIDPEWQRSRNASVGDTVKLFERPFKIVGVY"
     gene            complement(1805..>2729)
                     /locus_tag="VFO72_00740"
     CDS             complement(1805..>2729)
                     /locus_tag="VFO72_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477838.1"
                     /translation="DGLVLNSDGSLNMDRSFVRIDYFALLHGISVRNNVQRGITNRPV
                     DMIATRLPRELVLPLIEDKDIDEDVIWVAAAANRQALLLSRRDAAGQLSLRYLPISNL
                     KQDANGRLQFQSISWQADLPLRIYEDPNLNIPIADRTAWLTQWHTDQEWLEALHRTHY
                     SNGLIGLHEELARHRTERLSADDPTLDEQTRLMRNYERRKRELVEPDMLIVAQDHWNF
                     DVRGFNPGGNHGAFFRISTHSVFMVAGGRRTNIPRALDINKPYDSLSFVPTLLALTGD
                     LHDDNNPVPTLRERGFHRFPGRVVKELLPEW"
BASE COUNT          618 a          757 c          782 g          572 t
ORIGIN      
        1 gtaaacgccg acaatcttga acggtcgctc aaataactta accgtgtcgc cgacgcttgc
       61 atttcgactt cgctgccatt caggatcgat gatcgcttcg tcgcccgatt cgagttgccg
      121 gccttcacga atcgtgatgc gcgccagcgc ggagtactcg tcgtaattga tgccgtcaat
      181 caagcgctga ccaaaacccg agtcgctctt gtcgagtgtt tggcccaccg gtgttgccgc
      241 ccggacaccc ggaatagctg ccagttcagc ggcgtgagca acgggcaaac ggaactgaga
      301 tccgccacca agcccaacag atccagacgc gcgaatcatg atctcggcgc cgatgtttga
      361 ttcgcgccgg ccacgctcgt gcaacacacc atgcgccaaa ccaacggtga aaacgatcag
      421 cagcacgccg accgcggtgc cgagaacact gacagcggtg cgggctggtc gatgagcaat
      481 gtttgagaat acgaggctat ccataagacg ttgtaagcgt ggactacgat tcaaaattcg
      541 ctgccagatg tttggtagcg tgcgccaggt tagtatgagc aggccgaccg aggtccaggc
      601 aacgatggcg gctttgcgca ccagcgccaa cgcgagtccc gcagcgggtg taaaaccaag
      661 acctttttgc agaatgactt cggtgccgcc ctcatagacg ccgattgttc ccggcacgaa
      721 tgagaacacg aagttaataa cttttgtgag cgactcgatg atgtaagcct gcgcccactg
      781 tggatcgaac cccaacatac gcagcgcgag aaaaacttcc acgacgctcg tcgcgtgtgc
      841 gaggaggttg caggcgatca tcacgaagaa ggcgcctggg tgatgtttgt agaagtcgta
      901 gactttcaat tcgatgtgat agatgtgatc gcgccgtttg agtatcacct tcggactcag
      961 tcgcaactgt gcgagtcgat cgatgatcca cgtcagcagc atcacacggc gcttcgcagc
     1021 tatggcgacg aacacaatgc cgagcgcagc cacgagcgca ataccggtca ggacgacatt
     1081 gacctcctgc ggaagcggat agaccatcag catcacgcag gcgccgctca gaatgaaaaa
     1141 gacgacggat aagttgtaga gcagattatc gacgacgaga gcgggaaccc cgtaggtgag
     1201 tggtactcgt ttgcgcaaga gcgccacctt cgtcgcttcg ccgagcaatg gaccagtaaa
     1261 ggtgagaaaa ctgattgctt cgccgccgag tcgtgctgcg aatgcctggc gaaaagtgat
     1321 ctgccggtgc tccgcgggaa cggccgcgcg catcgcgatg gtacggagga aatggcgcag
     1381 cccgctgagg cccaggatga cgaagaagcc tatgccgatg cgcagcagcg cgtcgaacag
     1441 aggctgcacg ccgacgcgat tgatgacgta gattagcaga gcgaggccga atacgaaggc
     1501 gagtgactgt agccaaaaga aggaacgacg agaggtgttc gcagcggaag tttctggttc
     1561 ttccgagagt tcttctgtat ccgcctcctg ccagtcttct acgaactctt ttctcgactc
     1621 aatcaagggg acccgttttc tccattaaac tcagagacct gtcaaactca ggttaccttt
     1681 gtccctggga ccgcagtcgt ctcgcctgct ggattcgtta gtagcaatct ttgaaaccgt
     1741 gcaggcgaaa cgcctgcggt ccccggttca ggcttctcgc gtcgcggtgt ttgtttaccg
     1801 gctgttacca ctcgggcagc agttctttga caacgcgacc ggggaagcgg tggaatcctc
     1861 tttcccgcag cgttggaaca ggattgttat catcatgtaa atcgccggtc aatgctaaca
     1921 gcgttggcac gaagctcaaa ctgtcgtacg gcttgttaat atcgagcgcg cgcggaatgt
     1981 ttgtgcgtcg accacctgca accatgaaca ctgaatgcgt cgaaatgcga aagaacgcgc
     2041 cgtgattccc accgggattg aagccgcgca cgtcgaagtt ccagtggtcc tgcgccacga
     2101 tcagcatatc gggctcgacc agttcacgtt tacggcgctc gtaatttcgc atcaagcgtg
     2161 tctgttcgtc gagagtggga tcgtccgccg aaagtctttc cgtccggtgg cgggcgagtt
     2221 cctcgtgcag accgattagt ccgtttgagt aatgtgttcg atgtagcgct tcgagccatt
     2281 cctgatcggt atgccattga gtcaaccacg cggtgcggtc ggctatggga atgttgagat
     2341 tcggatcctc gtagatgcgc aacggcaagt ccgcttgcca actgatcgac tggaactgca
     2401 aacgtccatt cgcgtcctgc ttcagattac tgatcggcaa atagcgcagg ctcaactgcc
     2461 cggcggcgtc tcgacgtgac aacagcagcg cctgacgatt ggcggccgca gcaacccaga
     2521 tcacgtcttc gtcgatgtcc ttatcttcga ttagcggtag caccagttcg cggggtaacc
     2581 gagtcgcgat catgtcaaca ggacgatttg tgataccacg ctgcacattg tttcgtaccg
     2641 agatgccgtg cagtagtgcg aagtagtcga tccgcacgaa gctgcggtcc atgttgagcg
     2701 agccgtcgct gttcaggact aacccatcc
//
LOCUS       DASTEY010000041         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1302796, whole genome shotgun sequence.
ACCESSION   DASTEY010000041 DASTEY010000000
VERSION     DASTEY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302796"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="VFO72_00745"
     CDS             complement(<1..1705)
                     /locus_tag="VFO72_00745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9477839.1"
                     /translation="MTPERWQKVEEIFQAAVDLLPDERARYIAQVCANDTTLKRDVES
                     LLSQHDSAGELLDRPFYGNTELNVFESFVEEEDPMLGRRLGSYRIEREIGRGGMGAVY
                     EASRADNEFNKRAAIKLVKRGMDTDFILRRFRKERQILAALDHPHIAGLLDGGTTDDG
                     LPYFVMEYIEGQPLYSYCDAHQLNIIERLKLFRSICDAVHYAHQKQVVHRDIKPSNVL
                     VTSEGVPKLLDFGIAKLLNPQLAGDITHDPTATAMRLMTPEYASPEQVQGAPTTPSTD
                     VYSLGVLLYELLTGHRPYRLVNRAPHEIARVICEEAPAPPSVIITRKEDLLPSLYAGD
                     EATTLKQLYVARGGTLESIRRALTGDLDNIVMQALRKEPEWRYQTAAQLRDDITRYLE
                     GHPVSDLPDPPHLGLLARPDTSNENSLAVLPMKLLDINQQEGSPDYLGTGLADALITR
                     LSALRSFAVRPTSSVLRYGGDSDPLRAGRELGVAFILDGRIRRSTERIRVTIQLLNVR
                     DETTAWAGQFDEQFTDVLNLEDVISSHVAEAILPHLTVDQRLRLAKRGTDNPQAHEAY
                     LR"
     gene            complement(1781..2353)
                     /locus_tag="VFO72_00750"
     CDS             complement(1781..2353)
                     /locus_tag="VFO72_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9477840.1"
                     /translation="MNKHPTDNVTKLLIELSNGDRAAVDLLLPVIYDELRKLAANYLR
                     RERPDHTLQPTALVHEAYLRLIDQTRVNWQNRAHFFGVAAQIMRRLLVDHARKHNAEK
                     RGQDFKKLSLDENIDRAVERSAELIALDDALKTLATFDEQKARMVELRYFGGLSIEET
                     ADVLGVTPTTIKRHWRAAKAWLHGEMQRNV"
BASE COUNT          503 a          662 c          753 g          559 t
ORIGIN      
        1 cgcgcaggta agcttcgtgc gcctgcgggt tgtctgttcc tcgcttggcg agacgcagcc
       61 gttgatcgac ggtcaagtgc ggcaggatgg cttcggcgac atgcgaggag atcacgtctt
      121 cgagattcag aacgtcagta aactgctcgt caaactgacc ggcccacgcg gtggtttcgt
      181 cgcggacgtt aagcaactga atcgtcacgc ggatgcgttc ggtcgagcgg cgaatccgtc
      241 catccaaaat aaatgcaacg cctaattcgc gacccgcgcg caacggatcg gaatcgccgc
      301 cgtaacgcag cacactgctg gtaggtcgca cggcaaaact gcgaagggcg ctgagacgcg
      361 tgatcaaagc gtccgccagt cctgtgccga gataatccgg actaccttcc tgctggttga
      421 tgtccaacaa cttcatcggc agaacggcga gcgagttttc gtttgacgtg tcgggccggg
      481 ccagcaggcc gaggtgtggc gggtctggca gatcggaaac aggatggcct tcgagatagc
      541 gtgtgatgtc gtcgcgcagt tgcgccgctg tttgatagcg ccattcaggc tctttgcgca
      601 gcgcctgcat gacgatgttg tcgagatcgc ctgtgagtgc gcggcggatt gattcgagtg
      661 taccgccacg agcgacgtag agctgtttca gcgtcgtggc ttcgtcaccg gcgtaaaggc
      721 tcggtaacag atcttcctta cgcgtgatga tgacactcgg cggcgccggt gcttcctcgc
      781 aaatgacccg cgcgatttcg tgcggcgcgc gattcaccag tcgataggga cgatgccctg
      841 tcagcagttc gtaaagcagc acgccgagcg agtagacgtc agtactcggc gtagtgggcg
      901 cgccctgcac ttgttccggc gaggcgtact cgggagtcat caaacgcatc gccgttgcgg
      961 ttggatcatg cgtgatgtcg ccggcgagtt gcgggttgag caacttagcg atgccgaagt
     1021 cgagcagttt gggaacgcct tcggaagtga ccagcacgtt tgaaggctta atgtcgcgat
     1081 gtacgacctg tttctggtgc gcgtagtgaa ctgcgtcgca gatcgaacga aagagtttga
     1141 gacgttcaat gatgttgagt tggtgggcgt cgcaataact gtagagcggc tggccttcga
     1201 tgtactccat tacgaagtaa ggcaggccgt cgtcagttgt accaccatcc aacagccccg
     1261 cgatgtgcgg atgatcgaga gcagcaagga tctgtcgctc tttgcgaaag cggcgcagga
     1321 tgaaatcggt gtccatgccg cgcttcacca gcttgatcgc cgcgcgcttg ttgaattcgt
     1381 tgtcggcgcg tgaggcttcg tagaccgcac ccatgccgcc gcgtccaatc tcacgctcga
     1441 tacgatagga accgaggcgg cggccgagca tcgggtcctc ttcttccacg aacgactcga
     1501 aaacgttcaa ctcggtgttg ccgtagaaag gtcgatccag aagctctccg gcgctgtcgt
     1561 gttgtgagag cagcgactcg acgtcgcgtt tcagagtggt gtcgttagcg cagacctgtg
     1621 cgatgtagcg tgcgcgttca tcgggcagaa gatcgaccgc agcctgaaag atctcttcaa
     1681 ctttttgcca gcgttccggg gtcatgtctt actggacttc ttcgatagcc aattaagaag
     1741 gacaactttg atgcagaacg gctggaacta gcaacgtacc tcaaacattt ctttgcattt
     1801 caccatgaag ccaggctttg gctgcccgcc agtgacgttt gatggttgta ggagtgacac
     1861 ccaaaacgtc tgctgtctct tcgatagaga ggccgccaaa gtaacgcaac tcaaccatgc
     1921 gcgctttttg ttcgtcgaat gtcgcgagcg tcttgagcgc gtcgtcgagc gcgatgagtt
     1981 cggcgctgcg ttcgacggcg cgatcgatat tttcgtccag cgagagtttc ttaaagtcct
     2041 gcccgcgctt ttctgcgttg tgtttgcgcg cgtgatccac cagcaggcgt cgcattattt
     2101 gagcggcaac gccgaaaaag tgtgcccggt tctgccagtt cactcgcgtc tggtctatca
     2161 gacgcaggta agcctcatga accagagcgg tcggttgcag ggtgtgatcg ggacgttcac
     2221 gccgcagata attcgccgcc agcttacgca gttcatcgta gatgacgggt agtaaaagat
     2281 caaccgcggc ccggtcgccg ttcgacaact caatcaatag tttagtgacg ttgtcagtgg
     2341 ggtgtttatt caatcgttaa ctctcctgcg cgagaggtaa gtataaggca gaaagaacgg
     2401 caccaaaagg ggcttaacag gacaggttct tttacttggg cgtgctcttc cctgggaccg
     2461 caggcgtctc gcctgca
//
LOCUS       DASTEY010000042         6977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_495210, whole genome shotgun sequence.
ACCESSION   DASTEY010000042 DASTEY010000000
VERSION     DASTEY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495210"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..426
                     /gene="hfq"
                     /locus_tag="VFO72_00755"
     CDS             130..426
                     /gene="hfq"
                     /locus_tag="VFO72_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET9477841.1"
                     /translation="MSDGKQAPQNIQDAFLNTVRREKTAVVVYLLNGAKLTGRIRSFD
                     KFSVLLESGSQEQLIFKHAISTISQSRRASGELHPHATEANREVRTDDASSPGA"
     gene            474..1142
                     /gene="tmk"
                     /locus_tag="VFO72_00760"
     CDS             474..1142
                     /gene="tmk"
                     /locus_tag="VFO72_00760"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9477842.1"
                     /translation="MPGTFITFEGIDGSGKSTQLRLLSTFLRAHRCDALITREPGGTA
                     LGLRLREALLDALEEVDPLTELLVFAADRAQHVRRLLLPALESGAVVISDRYADATVA
                     YQGAGRGFPPELISQIVQLATGGLKPDLTLLFDVSIDESTSRTTRRSSGKNAAAKAGR
                     DRLDIENAEFHARVRDAYLRIAEAAPERIRVIDTSGPVERTHERVKQIVVPFLQSRGH
                     LVNV"
     gene            1135..2202
                     /locus_tag="VFO72_00765"
     CDS             1135..2202
                     /locus_tag="VFO72_00765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET9477843.1"
                     /translation="MFNELTGNVRVKESLKRMITSGRLPGALLFAGEEGVGKKRFALE
                     VARALNCRTPKDGEACGVCSSCVRTVKLNYPEREDADEWTQIIWTDHPDVGLVVALKR
                     VLRVEQMRQIEKEANFRPFEGKARVFLIDEADKLNDASANALLKVLEEPPQTSHLILI
                     TARPAMLLLTILSRCQMIRFAPLTPAEIESHLLSNKLADSKTAKLRARAAGGSIGRAL
                     AGDMVTFTSQRKAMLGVLNALVMTNDRAQLLRAAEQLNEAQYKDEFEERLDVLETLIR
                     DAWMLSLGVKPELLVNEDLSPELEKISQRMDAGRAADWILQIEDMREQLIVNINRKVT
                     TDALFLTMAGDVPPQKRPKIR"
     gene            complement(2259..3338)
                     /gene="ispG"
                     /locus_tag="VFO72_00770"
                     /gene_synonym="gcpE"
     CDS             complement(2259..3338)
                     /gene="ispG"
                     /locus_tag="VFO72_00770"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET9477844.1"
                     /translation="MRKSRPVQVKNIQVGGGAPVVIQSMTKTDTSDVGATVAQIEEMV
                     RAGCEIVRLAVPDNEAAVALKEIRKRVQEVPLVADIHFHYKLALMALEAGIDKLRLNP
                     GNIGAHQRIREVVRAAQAQHVPIRIGVNGGSLEKDLLKKYGTATAEAMVESALRHVQI
                     LEDLDFTDIIISLKASDVQRTVAAYRLLAEKVDYPFHLGVTEAGTSFSGTVKSSIGLG
                     ILLHEGIGDTIRVSLAAEPQEEVRVAWEIVKSLGLRTRGVTVVACPTCGRLDVDNFVG
                     IVEEIERRLAHIEEPLHLSIMGCAVNGPGEAHDSQLGITFGRGVGMIFKDGVPMRKVA
                     GENIVEAFVMETEKLLAELKHQPQI"
     gene            complement(3351..4739)
                     /gene="rseP"
                     /locus_tag="VFO72_00775"
     CDS             complement(3351..4739)
                     /gene="rseP"
                     /locus_tag="VFO72_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET9477845.1"
                     /translation="MTYLIVPLAFIFILGSAVVLHEFGHFIVAKLFGIRVETFSVGFG
                     PRLFGKKWGDTDYRVSAIPLGGYVKLGGDESNAPLEGAGAQNIPPEQMFNLRPRWQRI
                     LVALAGPVMNILTALAIPFAGALMYGVPATPAPVVYHVHKDGAADKAGLQPGDRIVSF
                     NGVKNPSWDSIYGDGLLSPGHALPVVVNRNGQEVHLTIIPTARTENGETAGFLDFVPD
                     YGGLPIVVSSIAPDSPAAEAGFKEGDRLLKVGDDNVKSSAQVTEYIRSHDAQKIPITV
                     ERNGQTITITTPERRLEGGRLGIGVGEIFPLHPAGIGSAATYAYNSNVQILSLTAKAL
                     GQVFTGKRSVRNTLSGPIGIYQAAQKSVERLGWDGVFGMLGFLSLNLGIFNLLPIPVL
                     DGGAIFLLLVEGMLATIGLSISTAVRDRIQQVGFVMVLLLMVFVITNDVLKTFGGSSE
                     DEKPPAATAPAK"
     gene            complement(4829..6007)
                     /locus_tag="VFO72_00780"
     CDS             complement(4829..6007)
                     /locus_tag="VFO72_00780"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET9477846.1"
                     /translation="MDSTGIAILGSTGSIGCNTLRVIESLDSTRMRVVALAAGSNVER
                     LADQIATHLPVLVSVESDESAHELRAKLFERDVDLPRIVTGEAGLVEVATHSEADCVV
                     SATVGAVGFLPTLRALEAGKRVALANKETLVMAGELMTRAAKQSGAELLPIDSEHNAL
                     HQCLRGERREEVRRIILTASGGPFRTKNKAEMKNASVSEAMRHPTWNMGAKITIDSAT
                     LMNKGLEVIEAHWLFGFGPEEIDILVHPESVVHSMIEMVDGSVIAQMGITDMRHAIQY
                     ALTYPERHSCQLPPLDLTAISRLHFEAPDLERFPCIPLAYRALSEGGTLPAAMNAANE
                     EAVSAFIDQQISLTEIPQVVETVMNSHVNQPAHDIETILAADHRARLAASEVICAIHG
                     "
     gene            complement(6233..6697)
                     /locus_tag="VFO72_00785"
     CDS             complement(6233..6697)
                     /locus_tag="VFO72_00785"
                     /inference="COORDINATES: protein motif:HMM:NF020279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly factor Coa1 family
                     protein"
                     /protein_id="HET9477847.1"
                     /translation="MTTKKIVLIVAAVVVVLALLAAVFVAGIVGFTLYQVGNSEAAVK
                     ARDFLRNNEQLKADIGEVKDFGSFITGSVHINNNNGEATLKLKVIGERKTVNASVDLV
                     YTKGSAWRVSGASYVDSSGQTIDLLDPYDSQLFIPRLIAWRGLHKRSGTAST"
     gene            complement(6697..>6977)
                     /locus_tag="VFO72_00790"
     CDS             complement(6697..>6977)
                     /locus_tag="VFO72_00790"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9477848.1"
                     /translation="GGVAAALVMAWISHYWFFPELPLKYGLPLAVVMTVFGILGDLSE
                     SALKRGAGAKDAANILPGHGGLLDRLDSLLFNAPLIYYFARFYFHGLG"
BASE COUNT         1602 a         1999 c         1891 g         1485 t
ORIGIN      
        1 catcgcgggt ggaccacggt ccaaataatc atgtttctct cacatctccg cctgactctt
       61 gtaagtccgg agggcgtgtt gtaatctgat tgcaaccaac gcttaacgga tccataccgg
      121 actcaaaaaa tgtcggacgg caaacaggca ccacagaaca ttcaggacgc ctttctcaat
      181 actgtgcgtc gcgagaaaac ggcagtggtg gtctaccttt tgaacggggc caagctgacc
      241 gggcgcatcc gcagcttcga caagttttcc gtgctgcttg aatccggctc ccaggaacaa
      301 ctgattttca aacacgccat ctccacgatt tcacaatcac gccgcgcctc cggcgaactg
      361 catccacacg caaccgaagc caatcgcgaa gttcgcaccg acgacgcaag ctcgcctggc
      421 gcgtgacatt cggctaaact ctcgccgaat tttcacccaa cccagaggga catgtgcctg
      481 gaactttcat taccttcgaa ggtatcgacg gaagcggaaa atcgactcaa ctaaggctac
      541 tgagcacttt cttacgcgcc caccgttgcg acgcattgat aactcgcgaa cccggcggca
      601 ccgcgttggg cctgcgactg cgcgaggcgc tgctcgatgc acttgaagag gtcgatccgt
      661 taacggagct tctcgtcttc gccgcagatc gcgcgcaaca cgtgagacgt ctgttgcttc
      721 cggcgctgga aagcggcgca gtggtgattt ccgatcgcta tgccgacgcg accgtggcgt
      781 atcaaggcgc cggacgcggt tttcctcccg aactgatttc gcagatcgtg caactggcca
      841 ccggtggttt gaagccggat ctgacgctgc tgtttgacgt ttcaatcgac gagtcgacca
      901 gccggacgac acgacgctct tcggggaaga atgcggcggc aaaagcgggc cgcgatcggc
      961 tcgatatcga gaacgctgag tttcatgcgc gcgtgcgcga cgcctatttg agaatcgccg
     1021 aggctgcgcc cgaacgaatc cgagtgatcg acacaagtgg tccagttgag cgtacgcacg
     1081 aacgagtgaa gcagatcgtc gttccgtttt tgcaaagccg aggccatctc gtaaatgttt
     1141 aacgaattaa cgggtaacgt tcgcgtaaaa gaatcgctca agcgaatgat tacgtccgga
     1201 aggctgccgg gagcgttgct ctttgcaggc gaagagggcg tcggcaagaa acgtttcgcg
     1261 ttggaagtcg cgcgcgcgct gaattgccga acgccgaagg atggcgaagc atgtggtgtc
     1321 tgttcgtcgt gtgtgcgtac cgtgaagttg aactatccgg aacgcgaaga cgcagatgag
     1381 tggacgcaga tcatctggac ggaccatccg gacgtcggac tcgtcgtcgc gctgaagcgc
     1441 gttttgcgcg tggagcagat gcgccagatc gagaaagaag cgaacttccg cccgtttgaa
     1501 ggcaaggccc gtgtgtttct gattgacgag gctgacaagc tcaacgacgc atctgctaat
     1561 gcactgttga aagtgctgga agagcccccg caaacatcgc atctgattct gatcaccgcg
     1621 cggccggcga tgttgctgct gacgattctt tcgcgttgcc agatgattcg attcgcgccg
     1681 ctgacacctg cggagatcga aagtcattta ctctccaaca agctcgcgga cagtaaaact
     1741 gcaaaacttc gtgcgcgtgc cgccggcggc agtatcggtc gtgctctcgc gggtgacatg
     1801 gttacgttta cttctcagcg gaaagccatg ctcggtgtgc ttaatgcgct cgtaatgacg
     1861 aacgatcgcg cccaactcct tcgagcagcg gaacaactca acgaagcaca gtacaaggac
     1921 gagtttgaag aacgactcga cgtgctcgag acgttgattc gcgatgcatg gatgttgtcg
     1981 ctgggcgtga agcctgagct tctggttaac gaagacttgt cacctgagct cgaaaagatc
     2041 agccaacgaa tggacgcagg ccgtgcggcg gactggatcc ttcagatcga agatatgcgc
     2101 gaacagttga tcgtcaacat caaccgcaaa gtgactaccg atgccttatt cctgacgatg
     2161 gcaggcgacg tgccgcccca aaagcgcccg aaaatccgtt aaaagccacg gagtaacacg
     2221 gatttcacgg ttcagcaaaa ccaaatctgt gtcgtccgtt aaatctgtgg ttggtgcttg
     2281 agttcggcga gaagcttttc ggtttccatg acgaaggctt cgacgatgtt ttctcccgca
     2341 actttgcgca ttggtacgcc gtctttgaag atcattccga cgccgcgtcc aaaggtaatt
     2401 ccgagttgtg aatcgtgcgc ttctccggga ccattcacgg cacaacccat aatcgagaga
     2461 tgcagcggct cttcgatgtg agcgagccgg cgctctatct cctcgacaat gccgacgaag
     2521 ttatcgacgt cgagccggcc gcaggtcgga cacgccacga cagtcacgcc tctcgtgcgc
     2581 agcccgagag acttcacaat ctcccacgcg acgcgtacct cttcctgcgg ttccgccgcc
     2641 aacgagacgc ggatcgtgtc gccgattcct tcgtgaagca gaatgccaag accaatcgaa
     2701 gacttgaccg ttccgctgaa cgacgtaccc gcttcagtaa ctcccaggtg aaatggatag
     2761 tcgaccttct cggccagcaa ccgataagca gccaccgtac gctgcacatc tgaagctttc
     2821 aacgagatga tgatgtcggt gaagtcgaga tcctcgagaa tctgcacgtg ccgcaacgca
     2881 gattcgacca tcgcttctgc cgtcgccgtg ccgtatttct tcagcagatc cttctcgagc
     2941 gacccgccgt tcacaccgat acgaatcggc acgtgctgcg cttgtgccgc gcgcacgact
     3001 tcgcgaatgc gttggtgcgc accgatgttg ccgggattga gacgcagttt gtcgatgccc
     3061 gcttcgaggg ccatcaaggc aagtttgtag tggaagtgaa tgtcggcaac cagcggtacc
     3121 tcctgaaccc gcttgcgaat ctctttgagc gcaacagccg cttcgttgtc cgggacagcg
     3181 aggcgaacga tctcgcaacc ggcgcgcacc atctcttcga tctgcgcaac cgtggcgccg
     3241 acgtcagacg tgtccgtctt cgtcatcgac tgaatgacta ctggcgcccc gccaccgacc
     3301 tggatgttct taacctgaac tggccttgat ttgcgcatgc ctcgagtgac ctatttggca
     3361 ggcgcggtag cagccggcgg tttttcgtcc tcgctactgc ctccgaaagt cttcaacacg
     3421 tcgttggtga tgacgaaaac catcaacaac aacaccatga caaaaccgac ctgttgaata
     3481 cgatcgcgaa ccgccgtcga gatcgacaag ccaatggtcg ccagcattcc ttcgacaagc
     3541 aacagaaaga ttgcgccacc gtcgagcact ggtatcggca gcaggttgaa gatgccgagg
     3601 ttcagactga gaaagcccag cattccgaac acgccgtccc agccgaggcg ttcgaccgat
     3661 ttttgtgccg cctgatagat tccgatcggg cccgatagcg tatttcgcac ggaacgtttg
     3721 ccggtgaaca cctggcccaa tgcctttgcc gtcaaactga gaatctgaac gttcgagttg
     3781 tacgcatacg tcgccgccga accgattccg gcgggatgga gcggaaagat ttcgccgacg
     3841 ccgattccca acctgcctcc ctcgagacgg cgctctggcg tggtaatcgt gatcgtttgg
     3901 ccgttgcgct cgactgtgat cgggatcttc tgcgcgtcgt gactgcgaat gtactcggtg
     3961 acctgagctg aactttttac gttgtcgtcg ccaaccttca acagtcgatc gccttctttg
     4021 aaaccagctt cagcagccgg tgagtcagga gcgatgctgc tgacgacgat tggtaaaccg
     4081 ccatagtcag gaacaaaatc gaggaaaccg gcagtttcac cgttctcagt gcgggcggtc
     4141 ggaatgattg tcaggtgaac ttcctgaccg ttgcggttaa caacgacggg gagcgcgtgg
     4201 cccggcgaaa gcagaccgtc gccatagatc gaatcccaac ttggattctt aacgccgttg
     4261 aaggagacga ttcgatcgcc gggttgaagt cctgccttgt cagcagcgcc gtccttatga
     4321 acgtggtaaa caaccggcgc cggagtcgcg ggcacaccgt acatcaacgc gccggcaaac
     4381 gggattgcaa gagccgtaag aatgttcatt accggaccgg ccagcgccac caggatgcgc
     4441 tgccagcgcg gccgcagatt gaacatctgt tcgggtggaa tgttctgcgc gccggctcct
     4501 tcgaggggcg catttgactc gtcgccgccg agctttacgt agccaccgag cgggatcgcc
     4561 gaaacgcggt agtcagtgtc gccccacttt tttccgaaaa ggcgcggacc aaaaccgacc
     4621 gagaaagttt ccactcgaat accaaacagc ttcgccacga tgaagtggcc aaactcgtga
     4681 agaacaaccg cagatccgag tatgaaaatg aaagcaagtg ggacgattaa gtaagtcatt
     4741 tagtgccttt tgtcagtatt tctgcccaaa aataaccacc tttagagtca cccactcaga
     4801 gccgggttgc ataggcagtg tacaggattc aaccgtggat tgcacagatt acctcgctgg
     4861 cagcgaggcg ggcgcgatgg tcggcggcga gaatcgtttc gatatcgtgg gccggttgat
     4921 ttacgtgact gttcatcact gtctctacga cttgaggaat ctccgtcaac gagatttgct
     4981 gatcgataaa tgcgctaacg gcttcctcat tagccgcatt catcgccgcc ggtagcgttc
     5041 ctccttcgct caaagcgcgg taagccagcg gaatgcaggg gaaacgttcc aaatcaggcg
     5101 cctcgaagtg caaacgcgaa atcgccgtca gatcgagcgg aggtaactgg cacgagtggc
     5161 gctcgggata cgtcagcgca tactggatgg catgccgcat atcggtgatt cccatctgcg
     5221 caatcaccga gccatcgacc atctcgatca tggagtgcac caccgattcc ggatgaacca
     5281 aaatgtcgat ctcttcaggg ccaaaaccaa ataaccagtg cgcttcgatg acttccagac
     5341 ctttattcat caacgtggcg gagtcgattg tgatctttgc gcccatgttc cacgtcggat
     5401 ggcgcatcgc ttcggagact gaagcgtttt tcatttcggc tttgttcttg gtccggaacg
     5461 gcccgcctga agccgtgaga atgatgcgcc gcacttcttc gcggcgttcg cctcgtaaac
     5521 actgatgcag cgcgttgtgt tcggagtcga tcggcaacaa ctcagcgcct gactgtttgg
     5581 cagcgcgagt catcaactcg ccggccatta cgagcgtttc cttattcgcc aacgcgacgc
     5641 gttttcctgc ttcaagcgct cgcaaagtgg gtagaaaacc gaccgcgcct actgtcgcgg
     5701 acacgacgca atcagcctcc gaatgcgtcg caacttcaac caaacccgct tctcccgtca
     5761 cgatccgcgg caagtcgacg tctctttcaa acaacttcgc acgcaactcg tgcgcagact
     5821 cgtcactctc gacggaaacc aataccggca agtgcgtcgc gatctgatcc gctaaacgtt
     5881 cgacgttgct accggctgcg agtgccacca cacgcatgcg cgttgaatcc agcgactcaa
     5941 tcacgcgcag agtgttgcat ccgatcgagc ccgttgagcc gaggatggca atgcctgtac
     6001 tgtccattga cgacctgatc aagcaattta cttcgttaag ctgctttgca caaaaaccac
     6061 gccgaaggcg ttcgcaaggt ctaagcccag ggtttatacc ctgggaacaa cgaaagtccc
     6121 cgcaatgtaa ctcggtaaga gttcgccaac tccttcggag ttgtgtagtt gaaggatgat
     6181 gttttcccca gggtataaac cctgggctcc aatttgcgaa ctccttcaga gttcaggtcg
     6241 aagcagttcc cgaccttttg tgcaagcctc gccacgcaat taagcgggga atgaatagtt
     6301 gggaatcgta ggggtcaaga agatcgattg tttgtcctga cgagtcgacg taagaagccc
     6361 cactcacgcg ccaggcactg cctttagtgt aaacgagatc gacagaagcg ttgacagttt
     6421 ttcgctcgcc gatgactttg agcttcaggg tcgcttcacc gttattgttg ttaatgtgaa
     6481 cgctgccggt aataaagctt ccgaaatctt tcacttcacc gatgtcggct ttcaattgct
     6541 cattgttgcg cagaaaatct ctggctttga cggccgcctc gctatttcca acctgataaa
     6601 gagtgaagcc cacaattccg gctacaaaaa cagccgcaag tagcgccagc acaaccacca
     6661 cggcggcaac tatcagcacg atctttttcg tggtcatcag cctaaaccat ggaaatagaa
     6721 gcgagcaaaa tagtagatga gcggtgcgtt gaagagcaag ctatccaaac gatcgagcag
     6781 gccgccatgt ccgggcagaa tgtttgcggc atctttagcg ccggcgccgc gtttcaacgc
     6841 gctctccgaa agatcaccga ggattccgaa gaccgtcatc acaacggcta acggtagtcc
     6901 atatttcaac ggcaactcag gaaagaacca gtagtgcgag atccacgcca tcacgagcgc
     6961 cgccgcgaca ccgccca
//
LOCUS       DASTEY010000043         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1269899, whole genome shotgun sequence.
ACCESSION   DASTEY010000043 DASTEY010000000
VERSION     DASTEY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269899"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VFO72_00795"
     CDS             <1..980
                     /locus_tag="VFO72_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9477849.1"
                     /translation="VLSFLQSAADPKVLSLVRLGLEHEMQHQELLVYDIKHLLCDQFD
                     APMKPLPEPSIKVEGMAEIEGGLFQLGYDSAQAQRSRPFAFDNEKPAHQVFLQDFAID
                     KALVSNGDFLEFINDSGYQNFRWWFSEGWETVNREQWRAPLYWELHDGEWMIRDLSGL
                     RPAASRKNEPVSHVSYFEASAYAKWAGKRQPTEAEWEKAACYDAARKVRRAFPWGDTD
                     PNSGNTNLFENGFWSVAPIGAFPDGANAYGCQQMIGDVWEWTTSDYMPYPGFKSEFDE
                     YNDKWFVNQKVLRGGSFATPQLHIRTTYRNFFHAHERWMISGFRCVATD"
     gene            1039..1833
                     /locus_tag="VFO72_00800"
     CDS             1039..1833
                     /locus_tag="VFO72_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9477850.1"
                     /translation="MSPKLRRLLATFVFVVFATAPALAQTAERSNADATTSATTTSSP
                     ARTVPVNPKYTTPKSPLDTAYGAKIAEYTTEKYFMTELVDHLPLSDKVPSPEKVLGYA
                     IGTPNKLTYTKDLYRYYRELAKASPRVRVFIAPEKSEEGREQMLVLVSDEANLQRLDR
                     YKEITAKLGDPRKITETEAHTLIAEGKPFYWASGSIHSPETGSPEMLMEMAYRLAVED
                     SPFIEDIRKNMIVMITPALEVDGRDRMVDLYYYRKANPGKPAPPLL"
     gene            1906..>2221
                     /locus_tag="VFO72_00805"
     CDS             1906..>2221
                     /locus_tag="VFO72_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477851.1"
                     /translation="MRTFLEYHPTVLHDLHESVPYLYTMTGTGPYNAWLDPLALDEFQ
                     VLAYHEVEEMTKRGVPGVWTHGFYDGWAPNYMLYIATGRNSIGRFYETFGNGGADTRE
                     RTL"
BASE COUNT          537 a          660 c          595 g          429 t
ORIGIN      
        1 aggtcctctc gtttctacag tcagcagcag acccaaaggt cctctcactc gtgagactcg
       61 gccttgagca cgagatgcaa caccaggaac tgctcgtcta cgacatcaag catcttttgt
      121 gcgaccagtt tgacgcaccc atgaagccgc tgccggagcc ttcgattaaa gtcgaaggca
      181 tggcggaaat cgaaggcggc ttgttccagc tcggatacga cagcgctcaa gcgcagcgtt
      241 cgagaccatt cgcgttcgac aacgagaagc ccgcgcatca agtattccta caggacttcg
      301 ccatcgataa agcactcgtg agcaacggcg actttctcga attcattaac gacagcggct
      361 atcaaaactt ccgttggtgg ttttccgaag gttgggaaac tgtcaatcgc gagcaatggc
      421 gcgcgccact ttactgggaa ctgcacgacg gcgagtggat gattcgcgac ttgagcggtc
      481 tgcgccccgc cgcgagcagg aaaaacgagc ccgtgtctca cgtcagctat ttcgaagcct
      541 cagcttatgc aaagtgggca ggaaagcgac agccgaccga agcggaatgg gagaaggcgg
      601 cgtgttatga tgcggctcgc aaagtcagac gtgcatttcc gtggggcgac actgacccca
      661 acagcggcaa cacaaatctt tttgagaacg ggttctggtc agttgcgccg atcggcgcct
      721 ttccagatgg cgcgaatgcc tacggttgcc agcagatgat tggcgacgtg tgggaatgga
      781 ccacttccga ttacatgcct tatccgggat tcaaatccga gtttgacgaa tacaacgaca
      841 agtggttcgt caatcagaag gttttgcgcg gcggctcgtt tgccacgccg cagttgcaca
      901 tccgaaccac gtaccgaaac ttctttcacg cccacgagcg ctggatgatc tcgggatttc
      961 gatgtgtagc cacggattag acggatctta aaaaagatct acacagatat caggcctgcc
     1021 taactgaagg agaacattat gtccccaaaa ctccgaagac tgctcgctac ctttgtcttt
     1081 gtggtgtttg ctactgctcc ggctctcgcg caaacggccg agcgcagcaa tgctgacgcg
     1141 acgacgagcg ccactaccac gtcgagcccc gcgcggactg tcccggtaaa tccgaaatac
     1201 acgacgccaa aatctccgct cgacacggca tatggtgcga agatcgccga atacaccacc
     1261 gagaagtact tcatgacaga gctcgtcgat cacctgccgt tgtctgacaa ggtgccgtct
     1321 ccggagaaag ttttgggata tgcgatagga actccaaaca agctcacata cacaaaagat
     1381 ctctatcgct actaccgcga gctggccaaa gcgtcgccac gtgtccgcgt cttcattgca
     1441 cccgaaaaga gcgaagaagg acgcgagcag atgcttgtgc tcgtcagcga tgaagcgaat
     1501 ctccagcgtc tcgatcgtta caaagagata acggccaaac tcggcgaccc gagaaagatc
     1561 actgaaaccg aagcccacac gctgatcgcc gaaggcaagc cgttctactg ggcctccggt
     1621 tccatacact cgccggaaac tggttcgccc gagatgctga tggagatggc ctatcgtctg
     1681 gccgtcgagg actcgccatt catcgaagac attcgcaaga acatgatcgt gatgatcacc
     1741 ccggcgctcg aagtcgacgg ccgcgatcgc atggtcgatc tctactacta ccgcaaagcc
     1801 aacccgggca aacccgcgcc gccactgctt tagtggggca aatacgtcgc acacgacaac
     1861 aaccgcgacg gccttggact ggcgctcgcg ctcacacgca accagatgcg gacgtttctc
     1921 gaatatcacc cgaccgtgct tcacgacctg cacgagtcag tgccgtatct ctacacgatg
     1981 acgggaaccg gcccatacaa cgcgtggctc gatccgctgg ccctcgacga gtttcaggtg
     2041 ctcgcctatc acgaagtcga agagatgacg aagcgcggtg tccccggcgt ttggacgcac
     2101 ggtttctacg atggctgggc gcccaactac atgctctaca tcgccaccgg acgcaactcg
     2161 atcggacgat tctacgagac cttcggcaac ggcggcgccg acactcgcga acgaaccctg
     2221 g
//
LOCUS       DASTEY010000044         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860 k141_907,
            whole genome shotgun sequence.
ACCESSION   DASTEY010000044 DASTEY010000000
VERSION     DASTEY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..2454)
                     /locus_tag="VFO72_00810"
     CDS             complement(28..2454)
                     /locus_tag="VFO72_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477852.1"
                     /translation="MTTLFQDLRYGVRILLKQPAVTLVAVVTLALGIGANTAIFSLVN
                     SILLRPLPFREPDRVVRLIQSSPKIGLATWGVSQADFGAYRDQNRSLESIALYNSSAV
                     NLTGQGEPERLPVTNVTADFFKVFGVNPLLGRTFVEGEDAQGKNTVCVISHAFWQRRF
                     GGDPNVVGKILNLNNTPTQIVGVMPSEFKFPRLEIDLWIPLPLDTKRTSPYTFAVVGR
                     LKPGTHLAQAQADTTGVLQNFGWQNPNLGEAVGLSEGNGPRTIVQPIKEVLLGKTEKP
                     LLVLLAAVALVLLIACANVANLLLARATSRTREIAVRVSLGATPRRVARQLLTESILL
                     SFIGAIVGVGLAAIGVRMLDRLPISGVVRMEEVSLNGRVLAFTAALTVLTGLLFGLMP
                     ALRAYSMGIDNGMREGARGTVSHRRLNSSLVAVQFALSLVLLIGAGLLLKSFQRLGAV
                     DLGFNAENTLTMVATLPRAKYDNNEKALRFYNSAIENLRNTPGIQHVGLTTNLPFADG
                     GNVDGIIVEGQEPPEGGNVSQLEQAEQQSVTPGTFQALGIPLLQGRDFQTSDHEKSPL
                     VAIVDEPLARRYWPAGDALGKRIQTTGGRQWLTIVGIVGGIKHLNLAEEKRPHIYFAV
                     AQSQALRASFVVRTDGPPLAAISTFRSAIKQVDPDMPLYLVRSMNEIIGTTLSTQRLT
                     NILLTAFAIIALTLAAVGIYSTMSVYVGSRTKEFGIRLALGAQPGVLRRAVLRQGMWL
                     TAVGVVTGIVGALVLTRTIKSLLFEVSATDPLVFTAIPVLLVIVSLVACYTPARRATK
                     VDPLVALRDE"
BASE COUNT          559 a          797 c          710 g          499 t
ORIGIN      
        1 cagcattgtt gctcctaccc gacccgctca ctcgtctcgc aaggccacta acggatcgac
       61 tttcgtggcg cgtctcgcgg gcgtgtaaca cgccaccaac gacacgatca ccagcagcac
      121 cgggatcgca gtaaagacca agggatccgt tgcactcacc tcaaacagca aactcttgat
      181 cgtgcgcgtc aataccagcg cgcccacaat gccggtgacc acacccaccg ccgtcaacca
      241 catcccctga cgcagcaccg cgcgccgcaa cacgcccggc tgcgcgccga gcgcgagcct
      301 gatcccaaac tccttcgtgc gactgccgac gtaaaccgac atcgtgctgt aaatcccaac
      361 cgccgccagc gtcagcgcga taatcgcaaa cgcagtcagc agtatgttcg tgagccgttg
      421 cgtgctgagc gtcgttccga tgatctcatt catcgatcgg accaggtaca acggcatatc
      481 aggatcaact tgcttgattg cactcctgaa cgttgaaatc gcagcgagcg gtggaccatc
      541 agtgcgcact acaaacgaag ctcgtaacgc ctggctctgc gccaccgcga aatagatgtg
      601 cgggcgcttc tcctccgcca gattgagatg tttgatacca ccgacaatgc cgacgatcgt
      661 caaccactgg cgaccgcccg ttgtctggat gcgttttccg agtgcatcgc cggccggcca
      721 ataccggcgc gccagcggct catcgacgat ggcgaccaga ggagactttt cgtggtccga
      781 cgtttgaaaa tcgcgaccct gcaaaagtgg aattcccagg gcctgaaacg ttcccggcgt
      841 tacgctttgc tgttcagcct gttctaattg cgagacgttg ccgccctctg gcggttcctg
      901 tccttcgacg ataattccat cgacgttccc gccgtcagcg aacggcagat tcgtcgtcag
      961 gccgacgtgt tgaataccgg gcgtgttacg caggttctca atcgcgctgt tgtaaaagcg
     1021 caacgccttc tcattattgt cgtactttgc gcgcggcaag gtcgcgacca tagtcagcgt
     1081 gttctccgcg ttgaagccga gatcgactgc tcccagccgc tgaaaacttt tgagtaacaa
     1141 accggcgccg atcaacaaca ccagggagag cgcaaactgc acggcaacaa gagagctgtt
     1201 caagcgccgg tgagagacgg tgccacgtgc gccttcacgc atgccgttat caattcccat
     1261 cgagtaggcg cgcagggcag gcattagtcc aaacaacaaa ccggtcagaa ccgtgagtgc
     1321 tgccgtaaag gcgagcacgc ggccattcaa actcacttcc tccatgcgca cgacgccgct
     1381 aatcggcagc cggtcgagca tgcgaacgcc aatcgcagcg agaccgacgc ctactatcgc
     1441 accaataaac gacagcaaaa tgctctccgt taaaagctgg cgcgcaactc ggcgcggcgt
     1501 agcccccagc gagacacgaa ccgcgatctc tctagttctt gaagtagcac gcgccagcaa
     1561 gaggtttgcg acgttggcgc atgcgatgag caacaccaga gcgaccgctg caagcaacac
     1621 gagcaacggt ttctcggtct tgccgagcag cacttccttg atcggttgaa cgatcgtcct
     1681 cgggccattg ccttcgctca gtccgacagc ttcgccgagg ttgggatttt gccagccaaa
     1741 gttctgaagc actcccgtgg tgtctgcctg cgcctgggcg aggtgcgtgc ctggtttcaa
     1801 acggcccacg acggcgaacg tgtaaggcga ggtgcgcttc gtgtcgagcg ggagcggaat
     1861 ccagagatcg atctcaagac gcggaaactt aaactccgag ggcatcacgc ccacgatctg
     1921 tgtaggcgtg ttattcaggt tcaatatttt tcccacgacg ttcggatcgc caccgaagcg
     1981 tcgctgccag aacgcgtggc tgatcacgca cacggtattc ttgccttgcg cgtcttcacc
     2041 ttcgacaaac gtacgtccga gcagtgggtt gacgccgaag actttgaaaa agtcggcggt
     2101 aacgttcgtc accggcaagc gttccggttc gccctggccg gtgagattga cggcactgct
     2161 gttatagagt gcgatcgatt ccagggagcg gttctgatcg cgatacgcac cgaagtcggc
     2221 ttgcgaaact ccccaggtgg caagaccaat cttcgggctc gactggatca aacgcaccac
     2281 gcgatcgggt tcgcgaaacg gcagtggccg cagcaggatg ctattcacga gactgaagat
     2341 cgcggtattc gcaccgatgc cgagcgcgag cgtcaccaca gcaacaagcg taaccgccgg
     2401 ctgcttcagc aaaatccgca cgccataccg gaggtcctgg aatagggtgg tcatctttgc
     2461 ctcgattcgg actctacttg ggtcgggtca gtaccgtaac gcggtagcgt cgggtttgtg
     2521 ttgccggtcg tgacccgacg ctaccgcgtt acggtactga cccga
//
LOCUS       DASTEY010000045         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1269923, whole genome shotgun sequence.
ACCESSION   DASTEY010000045 DASTEY010000000
VERSION     DASTEY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269923"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFO72_00815"
     CDS             complement(<1..318)
                     /locus_tag="VFO72_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9477853.1"
                     /translation="MRIVTAGLKKVVPMIHVPDVRATVAWYESIGFTVRATYGDETGE
                     NFSFAIVSFGETEVMFNTDGETSDKRRREVDLYVYTDEIDALYESLKSRVDVVEGPHD
                     TFYG"
     gene            339..1457
                     /locus_tag="VFO72_00820"
     CDS             339..1457
                     /locus_tag="VFO72_00820"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HET9477854.1"
                     /translation="MPTTPFPVATSRLLSLDVFRGITIAGMILVNNPGTWQHIYWPLQ
                     HAQWHGWTPTDLVFPFFLFIVGVAIALALGKRAEAGGSKRDLYLKIVKRALIIFAVGL
                     FLNAFPYFSLTELRIPGVLQRIAVCYFFASIIFLNTRARTQVLIAIGLLLLYWFLLAF
                     IPAPGYAAGDLTKEGSLPSFVDRVVFGKHVWAQAKVYDPEGLLSTIPALVTTLIGVLT
                     GHWLRTEKSRIEKVAGMFVAGAVLVALGWAWNSFFPINKALWTSSYVLFTGGLALQFL
                     GLCYWLIDIKGYRRWARPFEIFGMNAIALYVGAGLMAELFGVIRVGGRPLGGWIYQNL
                     FASWASPVNASLAFAIGFVLVWLFFMWLLYRRRIFIKI"
     gene            complement(1477..2394)
                     /locus_tag="VFO72_00825"
     CDS             complement(1477..2394)
                     /locus_tag="VFO72_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9477855.1"
                     /translation="MEHIRYSVAACQTDNPNPIDRRAMRANTDRILSLIDSAVIGASP
                     FLPVRLVVFPEFAHAAPVFETAAELIDKLAIPIPNEHTERLQQKAKEHDVYIQSGSML
                     EVDPKWPGHVFNTTCLIGPEGVLYKYRKVNTWLPFEVHTSPHDLPDYDEPLFPVADTP
                     IGRLGCAICYDWLFPEAMRQLTANGAEVLIRVSAYMDPWGATEPMSWWTIVNRCRALE
                     NMAYVVAANQGASLKHYPPYSWPGGSQVVDFDGRLLAEASPGPGERIVVAPIDISALR
                     HERATRRGHLMLGHLRTEAYPVYKEHFYP"
     gene            complement(2445..>3259)
                     /locus_tag="VFO72_00830"
     CDS             complement(2445..>3259)
                     /locus_tag="VFO72_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477856.1"
                     /translation="PAGDRMRLLLTGWTDYAWSSDNLAASQSGKALSLPALQVKDKRG
                     EWQTVIENVGIPVGRPQTVIADLTGKFLTASREVRIVTNMRIYWDRILVDTSNAEFPI
                     KVTRLAPTIADLRWRGFSHEYSPDGRQPMDYDYNDVSFTSPWKVMTGRYTREGDVRKL
                     LFASDDMFVISRPGDELVLSFNAAKLPPLPKGWTRTFLLYADGFSKEMDINSASPDQI
                     GPLPFHGMSKYPYTWPERYPLTPERKRYLETYNTRIVTSQVDRIAGLTGLRQ"
BASE COUNT          628 a          883 c          945 g          803 t
ORIGIN      
        1 gccgtaaaac gtgtcgtgtg gtccttcaac cacgtccaca cggcttttca aactctcgta
       61 tagcgcgtca atctcgtctg tgtagacata aagatcaact tcacgccggc gcttgtcgct
      121 ggtctcgccg tcggtattga acatcacttc ggtctcgcca aacgacacga tggcaaaact
      181 gaaattttcg cccgtttcat cgccgtaggt tgctcgaacg gtgaagccaa tactctcgta
      241 ccacgcgacg gttgcacgca cgtcagggac gtgaatcatg ggcacgactt ttttcaaccc
      301 ggctgtcacg attcgcattg tgctagaatt cgccgccgat gccaacaact cctttcccgg
      361 tcgcgacatc gcgcctgctt tcgctcgacg tgtttcgtgg tatcacgatc gccggcatga
      421 tcctggtcaa caatcccgga acatggcaac acatttactg gccactccag cacgcgcagt
      481 ggcatggttg gacgccgacg gatctggtgt ttccattctt cctgttcatt gtcggcgtgg
      541 caatcgcgtt ggcgctgggg aaacgcgcgg aggccggcgg cagcaaacgc gatctgtatc
      601 tgaagatcgt caagcgcgcg ttaatcattt ttgcagttgg actgttccta aacgctttcc
      661 catatttttc tttaaccgag ttgcgcattc ccggcgtcct gcagcgcatc gccgtttgct
      721 atttctttgc gtccatcatc tttctcaaca cgcgcgcgag aacgcaggtt ctgattgcga
      781 ttggtctgtt gctgctctac tggttcctcc tggcgttcat tccggcgccg ggatacgccg
      841 ccggcgatct gacgaaagaa ggaagtctgc cttcgtttgt cgatcgcgtc gtgttcggca
      901 agcacgtttg ggcgcaggcg aaggtttacg atccggaggg actgttgagc accattccgg
      961 cgctggtgac aaccttgatc ggtgtgttga ccggacattg gctgcgcact gagaaatcgc
     1021 ggatcgaaaa ggttgcgggc atgtttgttg cgggtgcggt gctggtcgcg ctcggctggg
     1081 cctggaactc tttctttccg atcaacaaag cgctgtggac cagttcgtac gtgcttttca
     1141 cgggcggtct ggctttgcag tttttgggtc tctgttactg gttgatcgat atcaaaggct
     1201 atcgccgctg ggccaggccg tttgagatct tcggcatgaa cgcgattgcg ctttacgtcg
     1261 gggcggggtt gatggccgag ttgtttggcg tgatcagggt aggcggccgg ccgctgggcg
     1321 gctggatcta tcaaaacctg tttgcgtcgt gggcgtcgcc ggtgaacgcc tcactcgcgt
     1381 ttgcgatcgg ctttgtcctg gtgtggttgt tctttatgtg gcttctttac aggcgacgaa
     1441 tctttatcaa gatttgagcc acggatctca cggatcttac ggatagaagt gttccttata
     1501 aaccggatac gcctcggtgc gcaggtggcc caacatgaga tggccgcggc gcgtcgcgcg
     1561 ctcgtgacgt aatgcagaaa tgtcgatcgg cgctacgacg atccgctcac ctggtccggg
     1621 cgacgcctcc gccagcaatc ttccatcaaa atcaactacc tgactgccgc ccggccagga
     1681 atatggcggg tagtgtttca aactcgcgcc ctggttagca gcgacaacgt aagccatgtt
     1741 ttccaacgcg cggcaacggt ttacgatggt ccaccaggac atcggttcgg tcgcgcccca
     1801 cggatccatg taagccgaga cacggatcaa gacctcggcg ccgtttgccg tcaactgtct
     1861 catcgcctcc ggaaacaacc agtcgtaaca gatcgcgcag ccaagccggc cgatgggcgt
     1921 gtcagccact ggaaacagtg gttcgtcata atccggcaga tcgtgcgggc tcgtgtggac
     1981 ctcgaacgga agccatgtgt tgaccttgcg gtacttgtag agcacgcctt ctggtccaat
     2041 caggcacgtg gtgttaaaga cgtggcccgg ccatttgggg tcgacttcga gcatcgagcc
     2101 gctttgaatg tagacgtcgt gttctttggc tttttgctgt agtcgctcgg tgtgctcgtt
     2161 ggggatcggg attgcgagct tgtcgatcaa ctctgctgct gtctcgaaca ccggcgccgc
     2221 atgtgcaaat tcaggaaaga ctacgagccg cacgggaaga aagggcgatg cgccaatgac
     2281 ggccgaatcg atcagcgaga gtatgcgatc cgtgtttgcg cgcatggcgc gacgatcgat
     2341 cgggtttggg ttgtccgttt gacaagcagc gactgaatat ctgatgtgtt ccataaaaat
     2401 actcgacagg acttacagaa cttacatgat agcgagtatt actttcactg tctgagtcct
     2461 gttaatcctg caatcctgtc tacttgagag gtaacgatgc gcgtgttgta ggtttccaaa
     2521 tatcgttttc gctccggcgt gagcgggtat ctttccggcc acgtgtacgg atacttgctc
     2581 atgccgtgaa aaggcagtgg tccaatctgg tcggggctgg cggagttgat gtccatctct
     2641 ttgctgaagc cgtcggcata gagcaggaag gtgcgtgtcc atccttttgg taatggcggt
     2701 agttttgcgg cgttgaacga tagcactagt tcgtcaccag gacgcgagat cacaaacatg
     2761 tcgtcggaag cgaagagaag tttgcgcacg tcgccttcgc gtgtgtagcg tccggtcatg
     2821 actttccacg gcgaggtgaa cgagacgtcg ttgtagtcgt agtccatcgg ctggcgacca
     2881 tcgggcgagt actcgtgcga gaagccgcgc cagcgcagat cggcgatggt tggcgccagc
     2941 cgagttacct ttatcggaaa ctcagcgtta gaagtgtcga ccaggattcg gtcccaatag
     3001 atccgcatgt tcgtcacgat ccgcacctca cggctcgcgg ttaagaactt accggtcaaa
     3061 tccgcgatca cagtttgcgg ccgccccact ggaatgccga cattttcgat aacagtttgc
     3121 cactcgccgc gtttgtcttt cacttgcaac gccggaagcg agagcgcttt accgctttgc
     3181 gacgccgcga ggttgtcact ggaccatgcg tagtcggtcc agccggttaa taacaaccgc
     3241 attcgatcgc cagccggca
//
LOCUS       DASTEY010000046         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_478897, whole genome shotgun sequence.
ACCESSION   DASTEY010000046 DASTEY010000000
VERSION     DASTEY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478897"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFO72_00835"
     CDS             complement(<1..365)
                     /locus_tag="VFO72_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9477857.1"
                     /translation="MTERSTLLSYLDDYEQRGDATVFAHHSGLRAVRWSYERLAHDAR
                     RFARELESQGVRRGDRVLLCGENSPEWAIAFWGCCLYGAVVVPLDKGSTPEFVNAVAQ
                     QTDANLLITDRDLLLPIRK"
     gene            472..1230
                     /gene="fabG"
                     /locus_tag="VFO72_00840"
     CDS             472..1230
                     /gene="fabG"
                     /locus_tag="VFO72_00840"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET9477858.1"
                     /translation="MLVQKPFTGKVAIVTGGTRGIGRAIAAELAHRGADVAFNYAQSV
                     EAADILKAELEAVGVRALAFQCHVGSTEAAAEMVKQVKEAFGRIDFLVNNAGITRDNL
                     ILRMKEEDWDEVIDTNLKGAWNFAKAALRVMLRQETGGSILNISSISGEIGMPGQSNY
                     SASKAGMIGLTKALAKEVASRKVTVNALALGMVATDMAGKLDESYQAKIIEQIPLGRF
                     AEADEVARIAAFLLSDDARYITGQVIQVDGGLAM"
     gene            complement(1234..2022)
                     /locus_tag="VFO72_00845"
     CDS             complement(1234..2022)
                     /locus_tag="VFO72_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477859.1"
                     /translation="MNLVILSVFLLLSVSASKAQTPASVDEKSQKIIDRAIEAVGGQT
                     YLNVQTVTSKGFYSEFKENVPQVPVKFLDYIAYPDSERTEFTSSGIRSIQTNVGDTGW
                     VFDGAVKKISDQTPAQVEDFKRTMRTSVENVLRGWWRNEGGKITYAGRREAGLAKRNE
                     TIRLTYPDGFWVEYEFGAKDGLPAKVIYKRSRKDLDTGDQVETTEEELLLKFITVNGV
                     TAPWVIDRFVNGKQTARINYESIQYNQRLADSLFAKPADVKSIK"
     gene            complement(2070..>2917)
                     /locus_tag="VFO72_00850"
     CDS             complement(2070..>2917)
                     /locus_tag="VFO72_00850"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9477860.1"
                     /translation="LKESHYKPDGVLLFLGPTGVGKTELAKAVAEFMFGDEEKMVRID
                     MSEYSDGTVSVEKLIGMPRGIVGSERGGILTEQLRENPYTVLLLDEVEKASPYLMNLF
                     LQAFDEGWLTDGRGKKVYLSDAIIIMTSNLGSESFKRFEKPLGFGTKTASDFRAVKNE
                     VMKAAEQRFSPEFRNRIDEIVVFSPLTMDEVRRIAKLYLDKVRRQMERQGKGVEITEA
                     AINKLTEDGFNTAYGARFLKRHIDEKVKLPITGMWKNSSRFSVDLEEGEVVVKPAGGS
                     NPGLN"
BASE COUNT          641 a          809 c          794 g          673 t
ORIGIN      
        1 attttacgga ttggtaaaag cagatcacga tcggtgatga gaagatttgc gtcggtttgt
       61 tgagcgactg catttacgaa ttccggagtg ctgcctttgt cgagtgggac taccaccgcg
      121 ccgtacaagc aacaacccca gaaagcaatc gcccactcag gactgttctc gccgcacaag
      181 agcacacgat cgccgcgacg aacaccttgc gattcaagtt cgcgagcgaa gcggcgcgcg
      241 tcgtgcgcga gccgttcgta agaccagcga acggcgcgca ggccgctatg gtgcgcgaag
      301 acagtcgcat cgccgcgttg ttcgtaatca tcgagataac tgagaagtgt gcttcgctcg
      361 gtcatgcccg tttggtctct tttgctcaca tgtgatatta gagaccgctc cgaaccaaga
      421 aacaagcagc catgtgacac gcaaggtagt gtccagaaag ggtcaaccaa gttgctcgtt
      481 caaaagccat ttaccggtaa agtggcaatc gtgaccggcg gcacgcgcgg tatcggccgc
      541 gccatcgccg cggagctcgc gcatcgtggc gccgatgtcg ctttcaacta tgcgcagagt
      601 gttgaagcag cagacatttt gaaagcggag ctcgaagctg tgggcgtgcg cgcgttggct
      661 tttcaatgtc acgtcggcag caccgaagca gcggcggaga tggtgaaaca agtgaaagaa
      721 gccttcggcc ggatcgattt cctggtcaac aacgccggca tcacgcgcga caatctgatc
      781 ctgcgaatga aagaagaaga ctgggacgag gtgattgaca cgaatctgaa aggcgcgtgg
      841 aattttgcaa aggccgcgct gcgcgtcatg ctgcgacagg agaccggcgg ctcgattctc
      901 aatatctcgt ccattagcgg cgagatcgga atgccgggcc agtcaaacta ctccgcgtca
      961 aaggcgggaa tgattggtct cacaaaggca ctggcgaagg aagtggcgag ccgcaaagtg
     1021 actgtgaacg cgcttgcgtt gggaatggtg gcgaccgaca tggccggcaa actggacgaa
     1081 agttatcagg ccaagatcat cgagcagatt ccgttgggcc ggtttgcgga agcggatgag
     1141 gtcgcgcgta ttgcggcgtt tctgctgtcg gacgatgcgc gttacatcac gggccaggtg
     1201 attcaggttg atggcggcct cgccatgtag gtgtcactta atactcttaa cgtcggccgg
     1261 cttcgcgaac agagaatcgg cgagtctttg gttgtactga atcgactcgt agttgatgcg
     1321 cgcggtctgt ttaccgttaa cgaagcgatc gatcacccac ggcgcggtga cgccgtttac
     1381 ggtaatgaac ttgagtaaca gttcttcttc cgtcgtttcg acctgatctc cggtgtctaa
     1441 atccttacgg ctgcgcttgt aaatgacctt cgcgggcaat ccatccttcg cgccaaactc
     1501 atactcgacc caaaacccat ccggatacgt caacctgatg gtctcgttac gctttgccag
     1561 tccagcttca cggcggccgg cgtaagtgat ctttccacct tcatttcgcc accagccacg
     1621 cagcacgttc tcgacgctgg tgcgcatcgt gcgtttgaaa tcttccactt gcgcgggggt
     1681 ttggtcggag atttttttga ccgcgccgtc aaacacccaa ccggtgtcgc caacgttcgt
     1741 ttgaatgctc cgtatgccgg aactggtgaa ctccgtgcgt tcgctatcgg gataggcgat
     1801 gtaatcaaga aacttcaccg ggacctgggg cacattctct ttgaattcgg agtagaaccc
     1861 cttgctggtg acggtctgca cgttcaggta agtctgtccg ccaacggctt caatcgcgcg
     1921 gtcgatgatc ttctgtgact tttcatcgac agaggcggga gtctgtgctt tcgaggcaga
     1981 aacactcaaa agcaaaaaaa ctgacaggat tacaagattc acgagcttag gtactttcac
     2041 ccatcgtgcc aatcctgtaa tcctgtccat taattcagtc ccggattact gcctccggcc
     2101 ggcttgacta cgacttcgcc ttcttcaagg tcaacgctga agcgtgacga gttcttccac
     2161 attccggtga tcggcagttt caccttttcg tcgatgtgac gtttcaagaa gcgcgcgccg
     2221 taggctgtgt tgaagccgtc ttcggtcagc ttattgatgg ctgcttcagt aatttcaaca
     2281 cctttgccct gccgctccat ctgtcgtcgc actttgtcga gatataactt cgcaatcctc
     2341 cgcacctcgt ccatcgtgag tggcgagaac accacgatct catcgatacg attacgaaac
     2401 tccggcgaga agcgctgctc ggcggctttc atcacttcgt tcttcacggc gcggaagtcc
     2461 gaggccgtct tggtgccgaa gccgagcggc ttttcaaacc gcttgaagct ttccgaaccg
     2521 aggtttgaag tcatgatgat gatcgcgtca gacaggtaaa ctttcttgcc gcgtccatcc
     2581 gtcaaccagc cttcgtcaaa tgcctgcaag aacaagttca tcagatacgg tgacgccttc
     2641 tcgacttcgt caagcagcag caccgtataa ggattctcgc gaagttgctc ggtaagaata
     2701 ccgccacgct cggaccccac gatgccacgc ggcatgccaa tcagcttctc gacactcacc
     2761 gtgccgtcgc tgtattccga catgtcgatg cgtaccatct tctcttcgtc gccaaacatg
     2821 aactcggcga cagctttcgc caactcagtt ttgccgacac ccgttggacc aaggaacaac
     2881 agcacgccgt caggtttgta gtgcgattcc ttgagtg
//
LOCUS       DASTEY010000047         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_956760, whole genome shotgun sequence.
ACCESSION   DASTEY010000047 DASTEY010000000
VERSION     DASTEY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956760"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFO72_00855"
     CDS             <1..227
                     /locus_tag="VFO72_00855"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9477861.1"
                     /translation="LMTLPPFFDDPKQARAHFRKLRELRDELARNGAFGKRRGELSMG
                     MTHDFEVAIEEGATMVRVGTAIFGQRDAGN"
     gene            214..822
                     /locus_tag="VFO72_00860"
     CDS             214..822
                     /locus_tag="VFO72_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET9477862.1"
                     /translation="MLVISRTFLFFSWAVQAAIVAIILLMILRLIGDAADLNPFAWTS
                     RTLRRLTDVFVIPVRGSLRQVGVDPKFAPLVVILVSILLGYFLLQLLGTLAMTLSGVI
                     ASVQSGSLILVLGFILYGLISFYILLIFMRIIFSWGMVSYTNRVMRFLVDATEPLLAP
                     LRHLIPPLGRFDISPIVAFLILWLFQAAIAGTLLRGATPVTF"
     gene            759..1145
                     /locus_tag="VFO72_00865"
     CDS             759..1145
                     /locus_tag="VFO72_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET9477863.1"
                     /translation="MALPGCDCRHALAGRDAGNVLRTSSEDLIVHSLRNGQVVFKVHV
                     VPRSSRSEVVGEHNGSLRVRIAAPPVDGAANEELIRILAKTFNVSRSAVKILSGHGSK
                     LKQIGIEGSTNEIVARLTDPRLEGWS"
     gene            1136..>2199
                     /locus_tag="VFO72_00870"
     CDS             1136..>2199
                     /locus_tag="VFO72_00870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9477864.1"
                     /translation="MELSQVSHYRILERLGAGGMGEVYLAEDMKLGRKVAIKILSEEY
                     TTNKDRLQRFEQEASAASNLNHPNILTIHEVGTDSGRHYIATEYIDGLTLRRKALSSQ
                     MDIPEILDIAVQVASALEEAHAAGIVHRDIKPDNIMVRRNGYVKVLDFGLAKLTETVD
                     RSPSDAEAATRVLVQTDAGVVMGTSHYMSPEQARGKRVDARSDIWSLGVVLYELVTGR
                     TPFEGETSTDVIVGITQKEPPPLARYSPNVPAELDWIVMKALRKDREERYQTAKELLT
                     DLRRLKQRLEFQSELERSAPVGFTTSGSSDVPSSPTGSARAVPTSEKTVTHVSSAEYI
                     ATEIKRHKVAAAIIALLLLAG"
BASE COUNT          473 a          595 c          641 g          490 t
ORIGIN      
        1 gcttgatgac gctgccgccg ttttttgacg atccgaaaca agcccgggcc cactttcgta
       61 aactccggga actgcgcgat gaactcgcgc gcaacggcgc cttcggcaag cgccgcggcg
      121 aactgtcaat gggcatgact cacgacttcg aggtagcgat cgaagaagga gcaacgatgg
      181 tgcgagtcgg caccgcaatt tttggtcaac gagatgctgg taattagtcg cacgtttctg
      241 ttttttagtt gggccgttca ggcggcgatt gtcgcaatta tcctgcttat gatcctgcgg
      301 ctaatcggcg atgccgctga tctaaacccg tttgcctgga cgtcgcgcac gttgcgccgg
      361 ttgacggacg tgttcgttat tccggtgcgc ggctcgttgc ggcaggtcgg tgtcgatccg
      421 aagtttgcgc cactggtggt aatcctcgtt tcgattctcc tcggctactt cctgctgcaa
      481 ctcctgggca cgctcgcgat gacactcagc ggcgtgatag ccagcgtgca aagtggctcg
      541 ctaatcctgg tgctcggctt tattctttac ggcttaatat cgttctacat tcttttgatc
      601 ttcatgcgga ttattttttc ctggggcatg gtcagctaca ccaatcgcgt aatgcggttt
      661 ttggtcgatg cgacggagcc gttgctcgcg ccgctgcgac atctgattcc gccgctgggc
      721 aggtttgata tctcccccat agttgcattc ttgatcctgt ggctcttcca ggctgcgatt
      781 gccggcacgc tcttgcgggg cgcgacgccg gtaacgtttt aagaacgagc agtgaagatt
      841 tgattgtcca ctcgcttcgc aatggtcagg ttgtctttaa ggttcacgtc gtgccacggt
      901 cgtcgcgcag cgaagtggta ggcgagcata atggctcgtt acgcgtgcgt atcgcagcgc
      961 cgcctgttga cggcgcggca aatgaagagt taatccgaat tttggcgaag acgttcaacg
     1021 tttcgcgcag cgcagtgaaa attcttagcg gtcacggaag caagttgaaa cagatcggca
     1081 tcgaaggaag tacaaacgaa atagtggcgc ggctaactga tccaagattg gaaggatgga
     1141 gttaagtcag gtttcacatt accggattct cgaaagactc ggcgccggcg ggatgggtga
     1201 agtctatctc gcagaagaca tgaaactcgg ccgcaaggtg gcgatcaaga ttctcagcga
     1261 ggagtacacg accaacaaag atcgcttgca acgtttcgag caggaagcgt cggcggcttc
     1321 gaatctcaat catccaaaca tcctgacgat tcatgaagtc ggcactgata gcggacgcca
     1381 ctacatcgcc accgagtaca tcgatggatt gacacttcgc cgcaaagcgc tgtcctcgca
     1441 aatggacatt ccggagatac tcgatatcgc agtccaggtt gcgagcgcgc ttgaagaagc
     1501 tcacgccgcc ggcatcgtgc atcgcgacat caagcccgac aacatcatgg tgcgccgtaa
     1561 cggttacgtg aaggtgttgg acttcgggct cgcaaaactt actgagacgg tcgaccgaag
     1621 cccatccgac gcggaagcag cgacgcgagt tctggtgcaa acagacgcgg gcgtagtgat
     1681 gggaacgtct cattacatgt cgcccgaaca ggcacgcgga aaacgcgtcg atgcgcgtag
     1741 cgacatctgg agccttggcg tggttctgta cgagctggtg accggacgca cgccttttga
     1801 aggcgaaacg tctaccgacg tgatcgtggg gattacacag aaagaaccgc cgccattggc
     1861 gcgttactcg ccgaacgttc ccgccgagct cgactggatc gtgatgaagg cgttgcgtaa
     1921 ggatcgcgaa gagcgctatc aaacggccaa agagctcctc acagatttgc gccggctcaa
     1981 acagagactc gagtttcaat cggagttgga acgatccgcg cccgttggtt tcactacgtc
     2041 cggtagcagc gacgtcccct cctcgcccac gggctccgcg cgcgccgtac cgacgtctga
     2101 gaaaacagtg acgcacgttt ccagcgcgga atacatcgct actgaaatca agcgtcataa
     2161 agtagccgcc gcgataattg cgctgctgtt gctggcggg
//
LOCUS       DASTEY010000048         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_83286, whole genome shotgun sequence.
ACCESSION   DASTEY010000048 DASTEY010000000
VERSION     DASTEY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83286"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            674..1909
                     /locus_tag="VFO72_00875"
     CDS             674..1909
                     /locus_tag="VFO72_00875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9477865.1"
                     /translation="MKTRSASNVGADPICEFYTRHPYPPPLENLDRARDLWQDENVHR
                     AEFHLLWPHKEYRADFEVLVAGCGTWQSAKFALVHPAASVVAIDVSTTSLEHTEALKR
                     KYDLTNLETRQLPIENVAALDHQFDMIVCTGVLHHLVDPDAGLRSLRSVLKPDGAMYL
                     MVYAPYGRTGVHMLQEYCRKLGVGTSPQEINDLTQVLRVLPQHHPLVSMFRGAKDFLD
                     AGALVDAVLNPRDRTYSVPQLYDFVERNELKFGRWYWQAPYSPRCGAIADTPHAGRLA
                     AVPEREQYVLMELWRGLISNHDFVVHREDRGSGVRFEGEQFLRYVPVRRAWTRCIQEQ
                     LPPGAAAVLVNQTHLFNDLFLFINEPEKLLYEAIDGRRSIAEIVDTVKEKEVSQLAPV
                     FFEKLWWYDQVVFDTSKAE"
     gene            2029..>2917
                     /locus_tag="VFO72_00880"
     CDS             2029..>2917
                     /locus_tag="VFO72_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET9477866.1"
                     /translation="MTKKLSRRTFAKVSSAAALALTTELPSLGSSTAPQTASARTNDA
                     GRDFPRGFIWGTATASYQIEGAVKEDGRGASIWDTFSHTPGKVVDNANGDVATDHYHL
                     YKSDVQLMKALGVKAYRFSIAWPRVFPNGDGPPNPKGLDFYNRLIDELLANGIQPFAT
                     LYHWDLPQSLQDRFGGWEGRETSKAFADYAGYVAARITDRVKHIFTINEFGAFIEVGY
                     RWGIHAPGLKLAGLRLNQTRHHAVLGHGLAVQAIRASAKSGTRVGLAENITSPVPVVE
                     TPQHVAAAESAMREMNAQYL"
BASE COUNT          631 a          813 c          848 g          625 t
ORIGIN      
        1 agacggtgac ggtgtgctgg ctggctgctg ttgagcggct cccgctggag ttgctgttgg
       61 cgatggagca gggtcgtctt gtgcgaagac gactgtagcg gttatcagca gaaacaggat
      121 caagcggggg aatagagtga tattggctat ctgcattgaa cgtcctcggc gctcacgaac
      181 tgatcacacg agggatcaaa tcaggcgccc atgaaggatt gaactactta atgcgaggcc
      241 cgggactact gccggaaaga cacgatgaaa gcgcaaagct tttatcgcag ttactgagtg
      301 gaagtcaatt ctgttgtaac cgggggtagt gggtcagtct gaatttaacg ccgaaggcgt
      361 tagctaattc cagcccagcg ttgcttcaac cctgggggac agggatccga gatacagccg
      421 aacgctaaaa gcgttggcta gcacttcgcc accgctttcg ccaacgcttt cagcgtttct
      481 cactttttca attcctaatc ccagggttga agcaaccccg ggctggcatt agctaacgcc
      541 ttcggcgtag tcatccccgc ggtgttcacc tcaaactgac ccactacccg cctgcggtcc
      601 caaggttcaa cacacgcagg taatcactgt ataatcccaa gccttcggtt cgtttgaacc
      661 gcaggcacaa ttcatgaaaa ccagatccgc atcgaacgtg ggcgcagatc cgatctgcga
      721 gttctacacc aggcatcctt acccgccgcc gctggaaaac ctggatcgcg ctcgcgatct
      781 gtggcaggac gaaaacgtcc atcgggcgga gtttcatctg ttgtggcctc acaaagaata
      841 tcgcgccgat tttgaagtgc tcgtggccgg ctgcggaacg tggcaatcag caaagtttgc
      901 gctggttcat ccagccgcaa gcgtggtcgc catcgacgtc agcacgacaa gtctcgagca
      961 cactgaagca ctgaagcgaa agtacgactt aacgaatctc gaaacgcgac aactgccgat
     1021 cgagaatgtg gccgcgttgg accaccagtt cgatatgatc gtctgcactg gcgtgttgca
     1081 tcatctcgtc gatcccgatg cgggtttgcg ctcgctccgg tcagtgctga aacctgacgg
     1141 cgcgatgtac ttgatggtct atgcgcctta cggccgaacg ggcgttcaca tgcttcagga
     1201 gtactgccgg aaactcggcg tcggcacgtc gccacaggag atcaacgatc tcactcaggt
     1261 gcttcgcgtg ctgccgcaac atcatccttt ggtatcaatg tttcgcggtg cgaaggattt
     1321 tcttgatgcc ggcgcactgg tcgatgcagt gttgaatcca cgcgaccgca cgtactcggt
     1381 gccgcaactg tacgacttcg tcgagcgcaa tgaattgaag ttcggacgct ggtactggca
     1441 ggcgccttac tcacctcgat gcggtgcgat tgccgatacg cctcatgccg gccggttggc
     1501 cgcagtgccg gagcgcgagc agtacgtgct gatggaactg tggcgaggtt taatctctaa
     1561 tcacgacttt gtggtccatc gggaggatcg cggttcgggg gtccgctttg agggcgagca
     1621 atttctgcgt tatgtgccgg tccgccgcgc atggaccaga tgcattcagg aacaacttcc
     1681 gcccggcgcg gcggctgtgt tagttaacca gacccaccta ttcaacgatc tgtttctgtt
     1741 tatcaacgag ccagagaagc tgttgtatga ggccatcgac ggacgtcgca gcattgcaga
     1801 gatcgttgac acagtgaaag aaaaagaagt ctcgcagctt gctccggtct ttttcgagaa
     1861 actctggtgg tacgaccagg ttgtgtttga tacgtcaaag gcggagtaac ctcggaccgc
     1921 aggcgtctcg cctgctactc acggcagcgc acgattcgaa aggttgcagg ggagacgcct
     1981 gcgattccat gttttgaacg cgtcattgca caaaggaggc gtgaacgagt gaccaagaaa
     2041 ctctcgagac ggacatttgc aaaagtgtcg agcgctgcgg cgcttgcgct tacgactgaa
     2101 ctgccttcac tcggctccag cactgctccg caaaccgcgt ccgcgcggac gaacgatgca
     2161 ggacgagatt tcccgcgcgg gttcatctgg ggtacggcga ccgcctcata tcagatcgaa
     2221 ggcgccgtta aggaagacgg ccgcggcgct tccatctggg acactttctc gcacacgccc
     2281 ggcaaagttg ttgataacgc aaacggcgac gtggcaaccg atcactatca tctttataag
     2341 agtgacgtgc agttgatgaa ggcgcttggc gtaaaagcgt atcgtttttc tatcgcctgg
     2401 cctcgcgtgt ttcccaacgg cgatggtcca ccgaatccga aaggactcga cttctataac
     2461 cgtctcatcg acgagttact tgccaacggc atacagccgt tcgccacgct gtaccactgg
     2521 gatttgccgc aatcgctgca agatcggttt ggcggctggg aaggacgcga gacctcaaaa
     2581 gctttcgctg actacgcggg ttacgtcgcg gcgcgcatca ccgaccgcgt taagcacatc
     2641 ttcacgatca acgagttcgg cgcttttatt gaagttggct atcgctgggg gatacacgcg
     2701 ccgggcctga agctcgccgg cttgcgcctg aatcagacac gtcaccacgc cgtgctgggg
     2761 cacggactcg ccgtgcaggc gatccgcgcg agcgcgaagt ccggcacgag ggtcggtctc
     2821 gccgaaaaca tcacgagtcc ggtgcctgta gttgagacgc cgcagcacgt cgctgccgcg
     2881 gagagcgcga tgcgtgagat gaacgcgcag tatctca
//
LOCUS       DASTEY010000049         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_874355, whole genome shotgun sequence.
ACCESSION   DASTEY010000049 DASTEY010000000
VERSION     DASTEY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874355"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFO72_00885"
     CDS             complement(<1..246)
                     /locus_tag="VFO72_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477867.1"
                     /translation="MRIANVTDAQMAPNAQWIVYTVSAVDDDKNVSTLWLARMGADSF
                     TVTPAPRPQRSTAPYVDWPDIRATPRPLLPPGWNASNP"
     gene            complement(563..847)
                     /locus_tag="VFO72_00890"
     CDS             complement(563..847)
                     /locus_tag="VFO72_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET9477868.1"
                     /translation="MSENEVAGLIVDAALKIHRALGPGLLESVYQATLSAELNRRGLR
                     VAQQVGLPVFYEGIKINLGYRVDLLVDDKVIVEIKSVETLAPVHRKQVQT"
     gene            complement(990..>2002)
                     /locus_tag="VFO72_00895"
     CDS             complement(990..>2002)
                     /locus_tag="VFO72_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9477869.1"
                     /translation="DTASVSSMHAIAAAREGVEQRIREDGMSGRPDLPLLRVYVSEQA
                     HSSIEKAVITLGLGQRALCKIPTDRDFRMDARALASAIETDKREGHLPFCVVATVGTT
                     STSSIDPVPEIVPICEQHAMWLHVDAAYAGSAAVVPEFRSILAGCERADSLVTNPHKW
                     LFTPFDVSVLYCRRLDLLKRAFSLVPEYLRTPEQEKVRNGSDYGIQLGRRFRALKLWM
                     VIRYFGQEGLAARIREHCRLADLFAAWVEESADWELMAPHPLALVCFRACPSSLPKHD
                     LDALNEAIMHGVNSSGHAFLSHTRLNDKLTLRLSIGNIRTTEQHVRQVWDLLNEQLRR
                     AR"
BASE COUNT          469 a          579 c          540 g          414 t
ORIGIN      
        1 cgggtttgat gcgttccagc cgggcggcaa caagggtcgt ggcgtagcgc ggatgtcggg
       61 ccagtcgacg tacggcgcgg tgcttcgttg tggccgcggc gctggcgtaa cggtgaatga
      121 atcagcaccc atgcgagcga gccagagggt gctgacgttc ttgtcatcat cgacggcaga
      181 gacggtgtag acgatccact gtgcattggg cgccatctgc gcatccgtga cattcgcgat
      241 acgcacaata tcgtcgggct gtatggcccg gcgctgctgc gcgtaggaag gagtaaacag
      301 gcaacatgtt gcgagcacga gaagtgcgtt gaagtaaagt ttcccgccaa ggcgcagagg
      361 cgcaaagaaa agttttctcg ggagcacgag tggagtgcct gcgtgaagac aagctagtag
      421 ccgctttcct ttgcgccttt gcgcctttgc gggaaacctc tcttcactcc tgcaatcgat
      481 taacaaccct ctgtatccca ttcttaataa gctccacgtt gaaattgata agcaagccga
      541 gccgcaagtc catcaaccgc aattacgttt gcacctgctt gcggtgaacc ggcgcaagcg
      601 tctcaaccga cttaatctct acgatgactt tgtcatcaac cagaaggtcg acgcggtaac
      661 caaggttgat cttgatacct tcatagaaaa caggaagacc gacctgttga gcgactctta
      721 atcctcgtct gtttagctcg gcactaagcg tcgcctggta aacagattcg agcagtcctg
      781 gaccaagggc tctatgtatt tttagcgcgg catcgacaat taagcccgca acctcatttt
      841 cagacacgtg aggaactcct tgtgaggaaa gtgcactagt caaactgttt catgagtcga
      901 acgagattcc acttgcgttg aagttttcag gtcggacgcc gtttggacca cggacaatac
      961 aaaaaccacg gatagtagag cgcccagttt catcttgccc tccgcaactg ttcattcaac
     1021 aagtcccata cctgccgaac gtgctgttcg gtcgtgcgga tgttgccaat cgacaaacgc
     1081 aacgtcagct tgtcgttcag ccgcgtatgt gagagaaacg cgtgcccgct ggaattaacg
     1141 ccgtgcatga tcgcctcgtt gagcgcgtcg agatcgtgtt tcggcagcga agacggacat
     1201 gccctgaaac aaaccagcgc cagcggatgg ggcgccatca actcccaatc agccgattcc
     1261 tcaacccacg cagcaaacag atctgccaaa cggcagtgtt cgcgaatccg cgcagccaaa
     1321 ccctcctggc cgaagtaacg gatcaccatc cacaacttca acgctcgaaa acgccggccg
     1381 agttgaatgc cgtaatcgga tccgttgcgc actttctcct gttccggcgt gcgcaagtat
     1441 tccggcacta atgaaaacgc gcgcttcaac agatccaagc gacggcaata aagcaccgaa
     1501 acatcgaatg gcgtgaagag ccacttgtgc gggttcgtca ccagcgagtc ggcgcgttca
     1561 caaccggcaa gtatcgatct gaactccggc accacagccg ccgagccggc atacgccgca
     1621 tcgacatgca gccacatcgc gtgttgctcg caaatcggca cgatctccgg aaccgggtca
     1681 atacttgacg tcgatgttgt tcccaccgtc gcgaccacgc agaagggcag gtgtccttcg
     1741 cgtttgtcag tttcaatcgc cgacgccagg gcgcgcgcgt ccatgcgaaa gtcacgatca
     1801 gtcggaatct tgcacaacgc gcgctgtccc aaaccaagtg tgatcacagc cttttcgatt
     1861 gacgaatgcg cctgttccga aacgtagact cgcaacagcg gcaggtctgg acggccagac
     1921 ataccgtctt cgcgaatgcg ctgttcgact ccttcgcgcg cagcggcaat ggcgtgcatg
     1981 ctcgagaccg aagcagtgtc gt
//
LOCUS       DASTEY010000050         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1072264, whole genome shotgun sequence.
ACCESSION   DASTEY010000050 DASTEY010000000
VERSION     DASTEY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072264"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFO72_00900"
     CDS             complement(<1..523)
                     /locus_tag="VFO72_00900"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET9477870.1"
                     /translation="MPSFNRILLIVLDGAGIGAMPDAPEWGDAGSDTFGHILESRGVR
                     LPNLQRYGLGNIRPLANLPPIDNPVGSYGRCALRSNGKDTTTGHWEMAGIILERAFPT
                     YPDGFPQAIIDRFVRETDVPGVLGNIPASGTEIIKVLGEEHVKTRKPIVYTSADSVFQ
                     IAAHEEVIPLERLY"
     gene            complement(624..1811)
                     /locus_tag="VFO72_00905"
     CDS             complement(624..1811)
                     /locus_tag="VFO72_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9477871.1"
                     /translation="MSALTKHCILSVLLIGIFIIEISSQQAQRPRRVTNGTDQTTDTK
                     KNATEELDEGDVVRVDTQLVSVPAVVTDASGRPLSGLKPENFRIIEDGQPQSIANFGT
                     AETPFEIALLLDTSGSTRDDVALIRAAATSFIEALRPGDRVGIVAFNTSQTVREPIAT
                     VEVLAPLTSDRDRLRSALENVGASNGTPYYDALERVVDAIFREPPADEVRGRRAVVAL
                     TDGVDSASNSDFATAKLKLSRAGVACYFVQINTEDFVEDRLMKDCLDDGQLSLSQRQI
                     ERYRRLFFPTAKAEHFNNFCHMGPFERMSISRELYNLARREMNDLARVSGGRSFPAAT
                     LADARAAFARVAADIGTLYSLGYYPTNKARDGKFRTIKLEVKGVREKAQVRARDGYYA
                     PKQ"
     gene            2354..2428
                     /locus_tag="VFO72_00910"
     tRNA            2354..2428
                     /locus_tag="VFO72_00910"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2386..2388,aa:Val,seq:tac)
     gene            2458..2534
                     /locus_tag="VFO72_00915"
     tRNA            2458..2534
                     /locus_tag="VFO72_00915"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2492..2494,aa:Asp,seq:gtc)
BASE COUNT          765 a          946 c         1015 g          847 t
ORIGIN      
        1 cgtaaagccg ttcgagcggt atcacctctt cgtgcgcggc aatttgaaaa acagaatcgg
       61 cggaggtgta gacgatcggc ttacgcgtct tcacatgctc ttcaccaagc accttgatga
      121 tctctgtgcc tgaagcagga atgttaccga ggacgccggg cacgtcagtc tcacgcacaa
      181 aacggtcgat gatcgcttgc ggaaagccgt cgggataagt gggaaaagcg cgctcgagaa
      241 ttattccggc catctcccaa tggccggtgg tcgtgtcttt gccgttggaa cgcagagcac
      301 agcggccgta tgaaccaacc ggattatcga tgggcggcag gttggcgagt ggtcgaatgt
      361 tacccaggcc gtaacgctgt aagttcggca ggcgcacccc gcgcgattcg aggatgtgac
      421 caaacgtgtc ggagcccgca tcgccccatt cgggagcatc gggcatcgcg ccgattccgg
      481 cgccgtcgag cacaatgagt aagatccggt tgaatgatgg catttagtta attgccgatt
      541 gccgattgta aattgccgat ttgaagcggg tgttcagaag cgtacgaaaa tcggcaatcg
      601 gcaatcggca atcgacaatt tcgtcattgc ttcggcgcgt agtatccgtc gcgtgcgcga
      661 acctgggcct tctctctcac tccctttacc tcgagcttga tcgtgcgaaa cttgccgtcg
      721 cgcgctttgt tggtcggata gtagccgaga ctgtaaaggg tgccgatgtc ggctgccacg
      781 cgtgcaaacg cggcgcgcgc atcagccaaa gtcgcagcgg gaaaactacg cccaccggac
      841 acacgcgcga ggtcattcat ctcgcgtctg gccaggttgt aaagctcgcg gctgatcgac
      901 attcgttcaa acgggcccat gtggcagaag ttgttaaagt gttcggcttt ggcggtagga
      961 aaaaacagac ggcggtagcg ctcgatctgt ctttgcgaaa gcgaaagctg cccgtcgtcc
     1021 aggcagtctt tcattagtcg atcttccaca aagtcttcgg tattgatctg cacgaagtaa
     1081 caagcgacac cggcacgcga gagtttaagt ttggcggtag caaaatctga gttgctggcg
     1141 gagtcaacgc cgtcagtaag cgccacgacc gcgcgccttc ctcgcacttc atctgccggc
     1201 ggttcgcgaa agatcgcatc cacaactcgt tccagcgcgt cgtaataagg cgtgccgttg
     1261 ctcgcgccaa cgttctcgag tgcacttcgt aaccggtcac gatcgctcgt caacggcgcg
     1321 agcacttcta cggtcgcgat cggctcgcgg acagtttgcg aggtattgaa cgcaacgatg
     1381 ccaacgcgat cgccgggtcg caaggcttcg ataaaactcg ttgccgcggc gcgaatcaaa
     1441 gcgacgtcat cgcgggtcga gccggaggtg tcgagcagca acgcgatttc gaacggtgtt
     1501 tcagcggtgc cgaagttagc aatcgattgc ggctggccgt cttcgataat gcgaaagttt
     1561 tccggtttca aacctgacaa aggccggccg gaggcgtctg ttacgaccgc aggaacggag
     1621 acgagttgtg tatcgacgcg gacgacgtcg ccttcgtcca actcttcggt cgcgttcttt
     1681 ttggtgtcgg tggtttggtc cgttccgttc gtcacgcgac gcgggcgttg agcctgttga
     1741 gaggagatct caatgatgaa aatcccgatc aggagaacgc tcagaataca atgcttcgtt
     1801 agtgctgaca tagcgtaaaa ctagatgggc tctcgtaagg agaatataaa ccgccaacag
     1861 tggaaactgc aaaactaccg agcgccgggg tggtgtccta atctgaacgc cgaaggcgtt
     1921 atcgagttcc agcccagagg ttggagcttg cgacaaccct ggggactgat tggatgtttt
     1981 taccttttac gctgaaagcg ttggctaacc gcgaatttgc caacgccttc agcgttagga
     2041 gggacgctaa cgttcgaccc agggttggag caaccctggg ctgtaattag cgaaccgcct
     2101 ctggcgttca gattaggaca ccactgagcg ccggaagggg cgaacgtctg cgcgtcccgc
     2161 gctccgctcg tctctgcgtg ttctgaacac ttctgaggcc aaactgcgtc aattccttag
     2221 cttgacactc ctgagcgcgt cgcttagcat ggcgactcgc tgcggttcat cgctgtttga
     2281 gacgcgtgcc ctgccgggtt ttgaagagtt gtcgtggttg acaaagggct ggcgacaggg
     2341 ctggtcctga tgaaggcacg tacgtcaatg gtagacggct tcccttacaa ggaagaggtt
     2401 gggggttcga gtccctccgt gcctaccaca accacagcgc aatcgacagt tctttgcgga
     2461 gtcgtagttc agttggttag aacgccggcc tgtcacgtcg gaggtcgcgg gttcgagtcc
     2521 cgtcggctcc gccattcagg acagaggtcg ctggaagagg ttccggcggc cttttctctt
     2581 tgccaagtcg tgttagtaag tgcaagtaca tcaaacggat ggcggaaggt tgcggtgaga
     2641 gtttggtcct tccatgtcga gttcgaacac acgaaattca acaatttccg cttttcgctt
     2701 gaattttgct gtttgaacag aaaataggcc ttttttgaga gttctagtag gcgaactcca
     2761 tcttgcacat aatcatgagc ggcgttttca tactcggcga tctgatccgc taaacgcttc
     2821 tgagtctgtc gccactcccg cgccttacgt tcaaagaaat cgggttcgat aaagccatca
     2881 agtcgatcat cgtagagccg atcaaggcgg ttctggattt tgctttgctc atcttgcaag
     2941 cgagtaatcg cattctgtct gaaccgtttc tcgtcggcgt ggctctgatg cagggcatcg
     3001 acgatccaat ccataacttc atcatcgaac acaagtccct tgagtaagtc ggcaaagcac
     3061 gcttcaagca cttcttctcg tgcgtaaggt tcggggcatt ttcctttgtt gcctgtgcga
     3121 tggtagtaaa cgtatttgcc cttcttcttc tcgcctacga gggggcagcc gcagtgccca
     3181 caatgcacaa gacctgagaa tgtaaactcg tgtttggtta ctttctcttt ggtcgagtag
     3241 cggccatcga gaatctcctg tacacgctcg aatagtgccg ttgaaatcag cggctcgtga
     3301 ctgccgcgat atgttctgcc gttccaatca aaatcgccgg tgtaaacacg gttttgaagc
     3361 agtctatgta cgttcgatct tgacattgct tggcctttac gccgggaaac caaaccagct
     3421 aagtgagcgc gccttgtaac ttcgtctatg gaacactgac cagtggcata ccactcaaac
     3481 atctgcttga tgatgggtgc ttgcgtggga tcaggctcga gaagccgttt gccattccgc
     3541 cccattacgt ttctataacc cgttggcgcg cag
//
LOCUS       DASTEY010000051         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1006337, whole genome shotgun sequence.
ACCESSION   DASTEY010000051 DASTEY010000000
VERSION     DASTEY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006337"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFO72_00920"
     CDS             complement(<1..833)
                     /locus_tag="VFO72_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9477872.1"
                     /translation="MRPKIAILLLFSFAITTAAQTPERQQFIRTEAPVIALTRVRIID
                     GTGAAAFEDQTIIISGGKIQSFGPTKSITIPANAETLDLNGYTVLPGIVGMHNHMFFP
                     MGGSPPMYSNMGSSFPRLYLANGVTTIRTTGSVAPYTDLEIKRLIDAGRMIGPKMHVT
                     APYLEGKGAFTPVMHQLTDATDARRMVNFWADQGATSFKAYMNITRDQLRAVVEEARK
                     RGLKVTGHLCSIGFKEAAEIGIDNLEHGLLADSEFVVNKQPDQCPQGVSASLRQLDLK
                     SA"
     gene            872..1816
                     /locus_tag="VFO72_00925"
     CDS             872..1816
                     /locus_tag="VFO72_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9477873.1"
                     /translation="MTVEFVIAVAVLAGLILFLFQQARRPIRPRSSPWPKRIRVGLTA
                     VLLLLGGIVFWGFFIEPNRLVVRHETIQIDQWPRQLNDFRIAVLSDIHAGGEFIDKRK
                     IRTIVERTNALQPELIVILGDFIAGDGRRNPLRMQPEQFAPILKELRAPLGVYAVLGN
                     HDWWYDGRHVRRTLEENGIKVLDDEWSKVDARGTSLYLVGLADLWTRPKRVVTTVSQI
                     PRDGTIVALTHNPDIFPRLPDYVPLLLAGHTHGAQVRVPFIGYVVEPSRTSGERYVRG
                     HVYENGHHLFVTTGIGTSIVPVRFGVPPEIVLLTVKSP"
     gene            1813..>2061
                     /locus_tag="VFO72_00930"
     CDS             1813..>2061
                     /locus_tag="VFO72_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477874.1"
                     /translation="MNELQHVYWIGGGSGGGKSTIARRIAAEHGLSIYATDDMMTDHA
                     RRSSPEECPLLHKFMAMDLDERWVHRDPQIMFETFHWFR"
BASE COUNT          406 a          557 c          623 g          475 t
ORIGIN      
        1 gcgcttttca gatccaattg acgcagactc gcagacacgc cttgcgggca ttgatcgggc
       61 tgtttgttga cgacgaattc cgagtcggcg agcagtccgt gctcgaggtt gtcgatgccg
      121 atttcggcag cttctttgaa acctatcgag cacaggtggc cggtgacttt cagcccgcgc
      181 ttgcgtgcct cttccaccac tgcgcgcaac tgatcgcgcg tgatattcat gtaagctttg
      241 aacgaagtgg cgccctgatc agcccagaag ttgaccatgc ggcgggcgtc ggtggcatcg
      301 gtgagctggt gcatcacagg agtgaaggcg cctttgcctt cgaggtacgg cgcggtcacg
      361 tgcatctttg gtccaatcat gcggccggcg tcgattagcc gcttgatctc gaggtccgtg
      421 taaggtgcga cgctgccggt ggtgcgaatc gtcgtgacac cgttggccag gtacagtcgc
      481 ggaaagctgc tgcccatgtt ggaatacatc ggtggcgagc cgcccatcgg aaagaacatg
      541 tgattgtgca tgccgacgat tcctggcagc acggtgtaac cgttcagatc gagggtctcg
      601 gcgtttgctg gtatggtgat gctcttggtt ggaccgaacg attggatctt gccgccgctg
      661 atgatgatcg tttgatcttc gaacgcggcc gcgcctgtgc cgtcaatgat gcgcactcga
      721 gtcagcgcga taaccggcgc ctcggtgcgg atgaactgtt ggcgttcggg cgtttgcgca
      781 gcggtggtga tcgcaaacga gaagagcagc agaatcgcga tttttggtct catggtgtgg
      841 aagatatcag aattgtgtta tctgtgtcgt aatgacagtt gagtttgtaa tcgcagtggc
      901 tgtgctggcg ggcctgatac tgtttttatt tcagcaggcg cgccggccca ttcgtccgcg
      961 atcgtctccc tggccgaagc gcattcgcgt cgggctaacg gctgtgctcc tgttactggg
     1021 aggcatagtg ttttggggat tctttatcga accgaaccgg ttggttgttc gacacgagac
     1081 tattcagatc gaccagtggc cgcgccaact caacgacttt cgcattgctg tcctatcaga
     1141 cattcacgcc ggcggcgagt ttatcgacaa gcgaaagata cgaacgatcg tcgaacgcac
     1201 gaatgcacta caacccgagc tgatcgtaat actgggcgac tttattgccg gtgatggtcg
     1261 ccgcaatccg ctgcgcatgc agccggaaca gtttgctccg atcttaaaag aactccgcgc
     1321 gccgctgggc gtttacgcag tgctcggcaa tcacgattgg tggtacgacg gtcgtcacgt
     1381 gcgtcgcaca ttggaagaga acgggatcaa ggtactggac gacgaatggt ccaaagtgga
     1441 tgcgcgcggg acgtctttgt atcttgtggg actcgcagat ctttggacgc gtccgaaacg
     1501 cgttgtgaca actgtcagtc agattccaag agacggaacg atcgtcgcgt taacgcacaa
     1561 tcccgacatc tttccgcgtc tgcccgacta cgtgccattg cttttggcag gacatactca
     1621 cggcgcgcaa gtgcgcgttc cgttcatagg ctacgtggtc gagccttcgc gcacgtcagg
     1681 cgaacgttac gtgcgcggac acgtctacga aaacggccat cacctttttg taaccaccgg
     1741 catcggcacg agcatcgttc cagtccgttt tggtgtcccg cccgagatcg tcctgctcac
     1801 ggtaaagtcg ccatgaacga actccagcac gtctactgga tcggaggcgg aagcggtgga
     1861 ggtaaatcga ccatcgctcg acgcatcgcc gctgagcatg gtctaagtat ctatgcgacc
     1921 gacgatatga tgacggatca cgcccgccgc agctcaccgg aagagtgtcc gctgctgcac
     1981 aagttcatgg cgatggacct cgacgagcga tgggtgcacc gcgacccgca gatcatgttc
     2041 gagacgtttc actggtttcg c
//
LOCUS       DASTEY010000052         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_907444, whole genome shotgun sequence.
ACCESSION   DASTEY010000052 DASTEY010000000
VERSION     DASTEY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907444"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..481)
                     /locus_tag="VFO72_00935"
     CDS             complement(44..481)
                     /locus_tag="VFO72_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET9477875.1"
                     /translation="MAELETEVAYSDAHIVAYPVEYIAGIDLYNAGEFHAAHDAWEER
                     WMGEVGPQEKLFLQAMIQSAVAFHHLDIGRPGAARQMYLRAKEKFTRLGCSVFMSLDL
                     EEYQAQLDSALSWLLSVPDPRESQPSEIIQPKIRLLATVDEFE"
     gene            complement(474..1400)
                     /locus_tag="VFO72_00940"
     CDS             complement(474..1400)
                     /locus_tag="VFO72_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9477876.1"
                     /translation="MHDVIIIGAGPAGLSAAFWCDELGLDTLVLEQAEEIGGQLHRVY
                     NPISNYLGLKVRNGKELLEHFSADVDSAGFDLWTRVAIDSIDLRAKRISLRSGERLQS
                     IAVILATGIRPRTLNIPGEHEFAGKGMIESATRDHDLFAGEDVCVIGGGDAAVENALI
                     LAEVCPTVTLVHRGRKLRARREFVERLQPNHAVTVFTESVVTRIIGDDRVEAVEILRK
                     EGLKPFQLAVRGVLVRIGVEPNTELFREQLELDQKGFITVNGEQETRVPMVFAVGDVS
                     SPTAPTISGATGAGATAAKVIAARLNAQRIHG"
     gene            complement(1393..2430)
                     /locus_tag="VFO72_00945"
     CDS             complement(1393..2430)
                     /locus_tag="VFO72_00945"
                     /inference="COORDINATES: protein motif:HMM:NF042562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6687 family protein"
                     /protein_id="HET9477877.1"
                     /translation="MRFEFYTDALENTPKLSVDGTVPNSIHFSHWEGNETPAELKADT
                     STEIALNLVASPNRDLFTKGIDLVTNNHFDCDGVLSVWTMLNGERALPYRDLLIDAAE
                     AGDFSEHSSDDGVRVSIAIQGAEQSSPNNDDGSPLAQMLAGREMATRQVDNDALSYEL
                     LLPEVERLLTDVNAYEPLWRDGWKAVADALESFESGRSQVRIYEDSGISVVTLAPEIF
                     SGGGFSPTRHSAPFTAISKYARGELFLIGIPTEGGWFYRLDYPYYSWAETVVRPRIAR
                     RDLAAALQSLNEREASGNGSWKTDNREMTSAVKFLDGSGSLAPSGLGPDEVLEALTKN
                     KEQSTKLQQHA"
BASE COUNT          594 a          807 c          621 g          573 t
ORIGIN      
        1 aataacacca ttcgcgttaa gttaagaatc tttaatcacc ctctcactca aactcgtcta
       61 ccgttgccaa caaccgaatc ttcggctgaa ttatctcaga cggctgcgac tctcgtggat
      121 caggaacact caacaaccac gacaacgcgg aatcgagttg cgcctgatac tcctcaaggt
      181 ccagcgacat gaaaacactg catcccaaac gcgtaaactt ctccttcgca cgcaagtaca
      241 tctgccgcgc cgctcccggc ctgccgatgt ccagatggtg aaacgcaacc gccgactgga
      301 tcatcgcctg caagaacagc ttctcctgcg gcccaacttc ccccatccag cgttcttccc
      361 aggcgtcgtg cgccgcatga aactcgccgg cgttatataa atcgatgccg gcgatgtatt
      421 ccaccggata tgccacgatg tgcgcgtcag aatacgcgac ctcagtctcc agttcagcca
      481 tgtattcttt gcgcgttgag tcgtgcggcg atcacttttg ctgccgtcgc acccgcgccc
      541 gtagcgccac tgatcgtcgg cgctgtcggg ctcgaaacat ctccaaccgc aaacaccatc
      601 ggcaccctcg tctcctgctc gccgttgaca gtgatgaaac ctttttggtc caactctaac
      661 tgttcgcgaa acaactctgt attcggctca acaccaattc gtaccaacac accgcgcaca
      721 gccaactgaa acggcttcaa cccttccttg cgcaggattt cgaccgcttc aacacggtcg
      781 tcgccgatga tacgagtaac cactgattca gtgaaaaccg taaccgcgtg gttcggctgc
      841 aggcgctcga cgaactcacg gcgcgcgcgc agttttcgac cgcgatggac cagcgtaact
      901 gtcggacaga cttccgccag gatcaacgca ttctcgactg cggcatctcc gccgccgatt
      961 acgcaaacgt cctcaccggc aaacagatcg tggtcgcgcg tagctgattc tatcattccc
     1021 tttcccgcaa actcatgctc gcccggaatg ttcaaagttc gcggtctgat gccggttgcc
     1081 aggatcaccg cgattgactg tagtcgttca ccactgcgca acgaaattcg tttggcgcgc
     1141 agatcgatcg agtcgatcgc cacacgcgtc cacaaatcga agcctgcgct atcgacgtcc
     1201 gctgagaagt gttccaatag ttccttgccg ttgcgcactt tgaggccgag atagttgctg
     1261 atgggattgt aaacgcggtg cagttgtccg ccgatctcct ccgcttgttc caacacgagc
     1321 gtgtcgagtc ccaactcgtc gcaccaaaaa gccgctgaca gcccagctgg tccagcaccg
     1381 atgatgatca cgtcatgcat gttgttggag ctttgtactc tgctctttgt tcttagtcag
     1441 agcttcgagc acttcatcgg gtcccaaccc cgagggcgcc aacgatccgc ttccatctaa
     1501 aaacttcacg gctgacgtca tctcgcggtt atctgtcttc caacttccat taccgctggc
     1561 ttcgcgctcg ttgagagatt gcaaagccgc agccaaatct ctgcgcgcaa ttcgtggccg
     1621 cactaccgtt tcagcccacg aatagtacgg ataatcgaga cgatagaacc aaccgccttc
     1681 ggtaggtatt ccgatcagaa acagttcgcc gcgagcatac ttcgagatag ccgtgaacgg
     1741 cgcggaatgt ctcgttggac taaacccacc gccactgaaa atctcgggag caagcgtgac
     1801 gactgagatt ccagaatctt cataaatacg tacttgcgat cgtccacttt caaagctctc
     1861 aagcgcatcc gccacagcct tccaaccgtc gcgccaaagc ggctcatacg cattgacgtc
     1921 cgtaagcagt cgttcaactt ccggcaacag tagttcatac gatagcgcgt cgttgtccac
     1981 ctggcgggtg gccatttcgc gcccagcgag catctgcgcc aacggcgagc catcgtcgtt
     2041 gttcgggctg gactgttccg cgccttgtat cgcgatgctg actcgcacac cgtcatcgct
     2101 cgaatgctcc gaaaaatctc ccgcctcggc ggcgtcaatt aataagtcgc ggtatggcag
     2161 agcgcgttcg ccgttcagca tggtccatac agacaacacg ccgtcacaat cgaaatggtt
     2221 gttggtgacg agatcgatgc ctttcgtgaa caaatctctg tttggcgagg caaccagatt
     2281 caaagcaatt tctgtcgacg tgtcagcttt caactccgca ggtgtctcat tgccttccca
     2341 gtgcgaaaag tgaatgctgt tgggaactgt tccgtcgacc gacagcttcg gcgtgttctc
     2401 gagggcgtcg gtataaaatt cgaatctcat gccggagagt gtagcgcacg cgagccacaa
     2461 acacgtaaga taccggagcc atggaagaga tcactgcaac tgaactaaaa caacgcctcg
     2521 acaacggcga cgacattcaa atcattgacg ttcgcgaaga taacgaagtc gcgatcgcgc
     2581 gcattccaaa ctccg
//
LOCUS       DASTEY010000053         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_924066, whole genome shotgun sequence.
ACCESSION   DASTEY010000053 DASTEY010000000
VERSION     DASTEY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924066"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..689)
                     /gene="rdgB"
                     /locus_tag="VFO72_00950"
     CDS             complement(60..689)
                     /gene="rdgB"
                     /locus_tag="VFO72_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET9477878.1"
                     /translation="MSTISQSPTLLLGTHNAGKVREIQTILGDVPWCVRSLLEFERVG
                     IATESGDAYSENAVAKAQFYAHATELCALADDSGLEVEVLGGAPGVFSARYAGVGASD
                     ADRRKLLLAELADVPKEERKARFVCVVAIARPDGAVLNISEGVCNGMIAFAPRGEGGF
                     GYDPLFVPDGFEQTFAELADSIKNRISHRARALLGTREFLISERALFSL"
     gene            complement(686..2395)
                     /locus_tag="VFO72_00955"
     CDS             complement(686..2395)
                     /locus_tag="VFO72_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477879.1"
                     /translation="MKRLRIVFALALLLILVVAAWLWFSLPAKVDLADYAPADSLVYV
                     EFNSLNDVVREIRNTEAWRVAAPITNSGPTHTDASLLLAAARAGVGPVESVLFARAQV
                     ALIVVGLNTSEQEDTLKVRPEVALIAETHTSRWRTRSVMVEGVKRLADYAYGASSCVE
                     PAQDRFECSVHGTDRKLVGAIHGTAIVLANSDYALRTCLEVREGTRPSIRTDTEFLKA
                     RASVSTPQSLGFGYISSVNSAKLFAWAAPLLMGMAPGDEQMEQLLAVSAGKILRGIAW
                     TAVPSGGGIEDRFLFSLEPGVVARLQPAFETSQRDENFWKLVPGSFESLTIYRSREPA
                     AAWNSLDAAVSFKLDALPAVLFGSLLKSSLSVYGISNPKEALATLSPPLLTMKRSEGA
                     EGSVLIARVNDEARLRRSLEEGFKETSGQILQGLQAEPDPAQEFTAVLADGYAILGKT
                     ENMRPALVELRKSQTLITEEKKNAQDSARQSTAAIVTYSNDEARLTNFISTLLMIQGR
                     RLSSEELSQLENLVRRLTLAQTETRLSAAGIERKTQSAFGQFSTFIAFLRPSQSDSLA
                     RER"
     gene            2501..>2770
                     /locus_tag="VFO72_00960"
     CDS             2501..>2770
                     /locus_tag="VFO72_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477880.1"
                     /translation="MTYRHLSAQALAEHDYDSFLTELEKSLTDGRVDPNDVVRDTLFQ
                     IYFGPARVTPEDLPLAARTSYHTLDPRNITTEPEYYTDIDAKRYLE"
BASE COUNT          648 a          880 c          693 g          549 t
ORIGIN      
        1 accagtgaga aagctgacgc attctaaaag ccgcttgttg gctggtcaag caaccgcggt
       61 cacaggctaa aaagcgcacg ctcagaaatc aggaactctc tcgtcccaag cagcgcccgc
      121 gcgcgatggc taatccggtt cttgatcgaa tccgcgagtt ccgcgaacgt ttgttcaaat
      181 ccatcaggca caaacagcgg atcgtatcca aatccgccct caccacgcgg tgcgaaagcg
      241 atcatcccat tgcacacgcc ttctgatata ttcaacaccg cgccgtccgg cctcgcgatc
      301 gccacaacgc aaacgaagcg cgcctttcgc tcctccttcg gcacgtcagc aagttccgca
      361 agcaacaact tccgacgatc cgcatccgac gcgcctacac cagcatacct cgcagaaaac
      421 actcccggag cgccgccaag cacttcaact tcgagtcccg aatcatccgc aagcgcgcaa
      481 agttcagtcg catgcgcata gaactgtgct ttagcgacgg cgttctcaga atatgcgtcg
      541 ccgctctcgg tcgcaatgcc aacgcgttcg aactctagca acgatcgaac gcaccacgga
      601 acgtctccga gtatcgtttg aatctcacgc actttcccag cgttgtgtgt gccgagcagc
      661 agggtaggcg actgcgatat cgtgctcatc gctcacgcgc aagtgaatca gactgactgg
      721 gccgcaggaa ggcgatgaat gtactgaact ggccgaaggc agactgcgtc ttgcgctcta
      781 tgccggcggc actgagtcgc gtctcagtct gggccaaggt caaacggcgc accaggttct
      841 ctaactgcga caactcttca ctcgaaagcc tccgcccctg aatcatcagc agtgtcgaaa
      901 taaaattcgt gagacgcgct tcatcattcg aataagtaac gatcgccgcg gtgctctgac
      961 gcgccgagtc ttgcgcattc tttttctctt cggtgatcag cgtttgactc tttcgcagct
     1021 caaccaaagc cggccgcatg ttttccgtct taccgagtat cgcgtaaccg tccgcaagca
     1081 cggccgtaaa ctcctgcgcc ggatccggtt cagcttgtag tccttgcaga atttgtccgg
     1141 aagtctcttt gaaaccttct tcaagcgaac gccgcaagcg tgcttcgtcg ttgacacgcg
     1201 cgatcagaac cgatccctcc gcgccttccg accgtttcat cgtcaacaac ggcgggctca
     1261 gcgtcgcgag cgcctccttc ggattgctga tgccgtacac cgacaaactc gatttcaaca
     1321 gcgaaccaaa caacactgcc ggcagagcat ccagcttgaa cgacaccgca gcgtcgagcg
     1381 agttccacgc ggccgccggc tcacgactgc gatagatcgt caaagattca aaactacccg
     1441 gcaccagttt ccagaagttc tcatcacgtt gagacgtttc aaaagccggt tgcagccggg
     1501 ccaccacgcc aggctccagc gaaaagagaa accgatcttc aattccaccg ccggaaggaa
     1561 ctgcggtcca ggcgatgccg cgcaggatct ttcccgcact gaccgcgagc agttgctcca
     1621 tttgttcgtc gcctggcgcc atgcccatca acaaaggcgc ggcccacgcg aacagttttg
     1681 cggaattaac agacgagatg taaccgaaac cgagtgactg cggcgtcgac acactggcgc
     1741 gcgccttcag aaactcggtg tcggtgcgaa tgctcggacg cgtgccttcg cgcacttcca
     1801 gacaagtccg cagcgcatag tcgctattcg ccaggactat cgctgtgccg tgaatagcgc
     1861 caactagctt acgatcggtt ccatgtactg agcattcaaa acgatcctgg gccggctcaa
     1921 cacacgagga tgcgccatac gcgtaatcag ccagacgctt gacgccttcg accatcacac
     1981 ttctggtacg ccaacgcgac gtatgagtct ccgcaatcag cgcgacctcc gggcgtactt
     2041 tcagcgtgtc ttcctgttcc gaagtgttca agcctacaac aatcaacgcc acctgggcgc
     2101 gcgcaaacaa cacgctctcg actggaccaa cgccggctcg agccgccgcg aggagcaggg
     2161 aagcatcggt gtgagtgggt ccgctgttag tgatcggcgc tgccactctc caggcttcgg
     2221 tgttccgaat ctcccggaca acgtcgttga gactattgaa ttccacgtaa acgagcgaat
     2281 cggcgggtgc gtaatctgcc agatcgactt tcgcgggaag gctgaaccag agccaagcgg
     2341 caacaacgag aatcagcagc agggccagag caaaaacaat acgaagacgc ttcatctcca
     2401 gcgaatccta gctttcccac ctgcgacagg caagttgccc taactgaaat ctccgtttct
     2461 ctgtggtgaa aacctatgtt actttggctc ggctccgctt atgacttacc gtcacttgtc
     2521 tgcacaggcg ctcgcagaac acgactacga cagttttctg actgaactcg aaaagtcact
     2581 aacggacggg cgagtagatc cgaacgatgt agttcgcgac acgctcttcc aaatctactt
     2641 cggccctgct cgggtaactc cggaagatct tccgctggcg gcgcgcacga gttatcacac
     2701 actcgatccg cgcaacatca cgaccgaacc cgagtactac accgacatcg acgcaaagcg
     2761 ttatctggaa
//
LOCUS       DASTEY010000054         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_198735, whole genome shotgun sequence.
ACCESSION   DASTEY010000054 DASTEY010000000
VERSION     DASTEY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198735"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFO72_00965"
     CDS             complement(<1..227)
                     /locus_tag="VFO72_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477881.1"
                     /translation="MTGIQVLDRPASQSFYAVAGRLLFIQCFDPSLATLIGRLIAGWQ
                     LTAVSFPERNPDIKISFAAGEDLPTVPADLD"
     gene            complement(249..425)
                     /locus_tag="VFO72_00970"
     CDS             complement(249..425)
                     /locus_tag="VFO72_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477882.1"
                     /translation="MEKQKDIVNEENAGVTSGSVVAELIEPKKAFVEPSISVPVDVLE
                     ATTFFQISTSGATN"
     gene            complement(533..1294)
                     /locus_tag="VFO72_00975"
     CDS             complement(533..1294)
                     /locus_tag="VFO72_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477883.1"
                     /translation="MCFAPAQSQQQSAATDKKPAAKQPFVWTIKTRPILNLSLKAEKA
                     KMSEVAQALSEWLKIPVFLGPERTKETISIEFSELTLEPALQLMSPIVYVDYELDTGS
                     MNPPKPLGIYFFDANQGEPPVTAVVTGTHQSMLIEGNTEDGVEPKTDDEKKRQEEQPL
                     RVRYENGALTVKAKQQPLPLVMLKIGEELGIPVDIQNERMDPIDVDLSKLPVEDVVRQ
                     LSPHIKLYYRADLTRADKRALRMVLAEPAKTTPGP"
     gene            complement(1551..2750)
                     /locus_tag="VFO72_00980"
     CDS             complement(1551..2750)
                     /locus_tag="VFO72_00980"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9477884.1"
                     /translation="MSRATNRHENRGIAEHEREHELLLCCARTDSAPAVVQRMRELAA
                     SVQLDWDYLFQLARRHAVIPLLYLQLAREAADLVPSSNLAKLKRQYQENNARNLILTD
                     ELRRLIDFFNDAGIEAIPYKGPALALFAYGNLALRRFVDLDVIVRKSDVLRARDLLLA
                     EGYTPTKSLTPAQQELLLRTQHNLQLARDNGRLILELHWEVAPHLFASSVQENELWRN
                     LTTIELNGTRMKTLGADDLLFSLCVHGSRHLWERLGWICDIAELIGHHDLNWPGLLER
                     ARQTDAERMFLLGLHLARKLLQAQLPAAVQQRCDSDPRLESLAANVIEHLFNGPTHIP
                     ATSTEIFKYNISVRKSLAARARYFVYMLRPTDSDFGTRSLPAAFSFGYYLVRPFRLLF
                     RTKTTST"
     gene            complement(2743..>3289)
                     /locus_tag="VFO72_00985"
     CDS             complement(2743..>3289)
                     /locus_tag="VFO72_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET9477885.1"
                     /translation="GILPLLEEKRGIQKGFEYIAANRVMLTYELPLNEIVLDFYDRLK
                     SVSRGYASLDYNLAGYWESDLVKLDVLVAGEPVDALSLILHRSTAQPKGRALAEKMKQ
                     LIPRQLFEVPIQAAIGNKVIARETVKALGKNVIAKCYGGDITRKRKLLEKQKEGKKRM
                     KKVGRVEIPQEAFLAVLKVNE"
BASE COUNT          708 a          889 c          913 g          779 t
ORIGIN      
        1 tggtccaggt ccgcgggaac cgtcggcaag tcttcaccag ccgcaaaact gatctttatg
       61 tcggggttac gttccgggaa agagactgct gtcagttgcc acccggcaat cagtcgcccg
      121 atcaatgtgg ccaggctggg atcgaagcac tgaataaata acaaccgacc ggcgaccgca
      181 taaaagcttt gcgaggccgg tcggtcgagg acctgaatac ctgtcatgca cccggaattt
      241 gtcttgtctt aattggtcgc accgctggtt gatatttgaa agaacgtagt tgcttcaaga
      301 acgtcgacag gaacggagat cgacggttcg acaaaagcct tcttcggttc gattaattca
      361 gcgacaacac tcccactagt cacaccagcg ttttcttcgt taacgatatc tttctgcttt
      421 tccaagttag ctgcctcctg gggttgttgg tggttaatat atttacttga tcgactcatg
      481 gcaaccaaaa tttcggacca agtgcaggat caagcgtaca acaaaccttg catcaagggc
      541 ccggcgtggt cttggccggt tcggcaagca ccatccgcag ggcgcgctta tcagcacgcg
      601 tgaggtcagc tcgatagtag agcttgatat gaggggaaag ctgacgcaca acatcttcca
      661 cgggaagttt tgagagatcg acgtcgatgg gatccattcg ctcattctga atgtcgaccg
      721 gaataccgag ttcttcaccg atcttcagca tcacgagcgg caacggttgt tgttttgctt
      781 taacggttag ggcgccgttt tcgtaacgga cgcgcagcgg ttgttcttcc tggcgcttct
      841 tctcgtcgtc agtcttcggt tcaactccgt cttcagtgtt gccttcgatg agcatcgact
      901 ggtgggtgcc ggtcacaacc gcagttaccg gtggttcgcc ctgattagcg tcgaagaagt
      961 aaatcccgag cggtttcggg ggattcatcg aaccagtgtc gagttcgtaa tccacgtaga
     1021 caattggcga catcagttgc agcgcgggct cgagagtcaa ctcgctgaac tcgatcgaga
     1081 tcgtttcctt cgtgcgttct ggaccaagga acaccggaat cttgagccat tccgacaagg
     1141 cctgggccac ctccgacatt ttcgcttttt cggctttcaa agacaggttc aggatgggac
     1201 gggtctttat ggtccagacg aatggttgtt tggctgcggg tttcttatca gtcgccgcag
     1261 attgttgttg tgactgtgct ggcgcgaaac acacgacaag cagtaagacc gttgatgcgg
     1321 cagtcttcag acagtcacgg caactcttat gtttcacttt gtaggtcctt atcttttcgc
     1381 ggcgacgaac aatgtgctgt tcagacgggt tgaccgccga ttgcggtgaa acctagcacg
     1441 tgacaaaaaa tccgtcaaat aatgccccgg gagcgcaggc gtctcgacgg caactgacgc
     1501 gagcgctcga tcggccacgg ctgcaggcga aacgcctgcg atcccaggat tcaagtactg
     1561 gttgttttgg ttctgaagag gagccggaag ggacgcacca ggtagtagcc gaaactgaac
     1621 gcggcgggaa gcgaacgagt gccgaagtcg ctgtcggtcg gccggagcat gtaaacaaaa
     1681 tagcgagcac gcgcagcgag actctttcgc acactgatgt tgtatttgaa tatttccgtt
     1741 gacgtggcgg gaatgtgtgt tggaccgttg aagaggtgtt cgatgacatt cgcagccagc
     1801 gattcgaggc gcggatcgga gtcgcaacgc tgctgcactg cggccggtag ttgtgcctgc
     1861 aaaagttttc gagcaaggtg cagacccagc agaaacattc tttcggcgtc cgtctgtctg
     1921 gctctttcga gcaatcccgg ccagttcaaa tcgtgatgcc cgattagttc agcgatgtcg
     1981 caaatccagc cgagtcgttc ccacaaatga cgcgagccgt gcacgcagag tgaaaacaac
     2041 agatcgtcgg cgccgagagt tttcatcctc gtgccgttga gctcgatcgt tgtcaggttt
     2101 cgccagagtt cgttctcctg gaccgacgac gcgaacaagt gaggcgcgac ctcccagtgc
     2161 agttcgagaa tcaaccggcc gttatcgcgc gcaagctgta ggttgtgttg cgtgcgcagg
     2221 agcaattcct gttgtgccgg cgtgagcgac ttcgtcggtg tgtagccttc agcgagaagc
     2281 agatcgcgcg cgcgcagcac gtctgacttc ctgacgatta cgtcgaggtc cacaaagcgg
     2341 cgcaaggcga gattgccgta ggcgaagagc gcgagtgccg gacctttgta aggaatcgcc
     2401 tcgatgcccg cgtcgttgaa gaaatcgatc agccggcgga gttcgtcagt aagaatcagg
     2461 ttccgcgcgt tgttctcttg atactggcgt tttagtttgg cgagattgga agatgggacg
     2521 aggtcggcgg cctcgcgagc gagttgcaaa tacagcagcg ggatcaccgc atggcgccgc
     2581 gcgagttgaa agaggtaatc ccagtcaagt tgtactgacg ccgcaagctc gcgcatccgc
     2641 tgaacgacag ccggcgcgga atctgttcgc gcgcaacaaa gcaacagctc atgttcgcgc
     2701 tcgtgctcag caatgcctct gttttcgtgt ctgttcgtgg ctctactcat taaccttcaa
     2761 aacagccaga aacgcctcct gcggaatctc cacgcgaccg actttcttca tccgcttctt
     2821 gccttccttc tgcttctcca gcaactttcg tttccgcgtg atatcgccgc cgtaacactt
     2881 cgcaatcacg ttcttgccaa gcgctttcac tgtttcgcgc gcgatgacct tgttgccaat
     2941 tgccgcttgt atcggcactt cgaacagttg ccgcgggatc aactgtttca tcttttccgc
     3001 gagcgcccgt cctttcggtt gcgctgtgct gcgatgaagt atcaacgaca atgcatcaac
     3061 tggttcgcct gcaacgagca catccaactt caccagatcg ctctcccaat acccggcgag
     3121 gttgtaatcc agcgaggcat acccgcgcga aaccgatttc agccgatcgt aaaaatcgag
     3181 cacgatctcg ttcagcggca gttcatacgt cagcatcacg cgattggcgg cgatgtactc
     3241 gaagcccttc tgaattccgc gcttctcttc cagcaacggc aaaatgccg
//
LOCUS       DASTEY010000055         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_132996, whole genome shotgun sequence.
ACCESSION   DASTEY010000055 DASTEY010000000
VERSION     DASTEY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132996"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..982)
                     /locus_tag="VFO72_00990"
     CDS             complement(62..982)
                     /locus_tag="VFO72_00990"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9477886.1"
                     /translation="MTSAARIIGVFIVCLLSWQLAGTVFAQTDSPAPTRYQITLTDGY
                     SFEVDDVWKQADEIWYRQGAISKALPVSSVKKYGPLDAKAPPPKSVLPKPVAPEAPLA
                     IWIYLNGGARVRVDEVNETTDGAWYRRGNLSMFLDRERIARIEREQPQTGATAWRQGG
                     WTSGNDRIDHLIKINAARFGVDPYLVFCVIEQESQFKTYARSPKGAQGLMQLMPATAR
                     RLGVRKPYDPAENIMGGTRYLKELMTMFNGRVDLVLASYNAGEGAVMKYGRNVPPYRE
                     TRDYVKKIGKRYGIDGRKPPEDSNAPVPRR"
     gene            complement(994..2277)
                     /locus_tag="VFO72_00995"
     CDS             complement(994..2277)
                     /locus_tag="VFO72_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477887.1"
                     /translation="MGPPTPEEVSETGVHEAFLCDLALKHVAMLPEPTTISVADRMHL
                     PRALTEEILYHLYREKLIEMRLQSAVGATRYAMLDHGWERVRRLNQQSGYLGPAPVSL
                     DDYAHMMRLQAVPSRAATMENVRSAFKDLVLPDSLLQTLGCVINSRSSLFLTGLPGTG
                     KTAVSERMNSALSGTIWIPYAVEVDGQIIRVYDAHNHHAVKQQDSLIEHDRRGIEIQR
                     PLVIVAGELTLDNTDLTWSESAKFYEAPFQMKANGGTLVIDDFGRQRVSPTDLLNRWI
                     LPLEKRIDFLTLHSGKKIEVPFEELVIFSTNLDERDLVDEAFLRRMGYRARVEPPTAG
                     AFMEIFKRTATSRGIVVTQECLDHVLQKYAAEKRVMKGCEPRDLLNKVRDICLFEGRP
                     GQLTPELIDLAWGNYFGTAHGFAPEQKQFEVAQTA"
BASE COUNT          480 a          679 c          694 g          550 t
ORIGIN      
        1 gagacgcctg cggtcccagg ttaccggcgt cccggttcga ggctgaaatt tcaaacaatc
       61 gctatcttcg cggaactggt gcgttgctgt cttccggagg tttgcggccg tcaatgccgt
      121 accgcttgcc tatcttcttc acgtagtcgc gagtttctcg ataaggcgga acgttgcgac
      181 cgtacttcat aaccgcacct tcgcccgcat tataactcgc caggaccaga tcgacccggc
      241 cattgaacat cgtcatcagt tccttcaaat aacgcgtgcc acccatgatg ttttcggcgg
      301 gatcgtaggg cttgcgcacg cccaaacggc gcgctgtggc cggcatcaac tgcatcaatc
      361 cctgggcgcc tttcgggctg cgcgcatagg ttttgaactg cgattcctgc tcgatcacgc
      421 aaaacaccag gtaagggtcg accccaaacc gggcggcgtt aatctttatt aaatgatcaa
      481 tacggtcatt tccggaggtc cagccgccct gacgccaggc cgtagccccg gtttgcggct
      541 gttcgcgttc gatacgggcg atccgctccc ggtccagaaa catcgataaa ttgccccggc
      601 ggtaccaggc gccgtcggtg gtttcgttca cttcgtcgac gcggacgcgg gcgccgccgt
      661 tgaggtaaat ccaaatcgcc agaggcgctt cgggagcgac aggcttgggt aacacggatt
      721 taggcggagg ggctttggca tcgagaggcc catatttctt tacggatgac actggtaatg
      781 cttttgaaat ggccccttgt cgataccaga tctcgtcagc ttgtttccaa acgtcatcta
      841 cctcgaatga ataaccgtcg gtgagcgtga tctggtacct ggtcggagct ggagaatcag
      901 tttgcgcgaa gacagttccg gcgagttgcc acgacagcaa acaaacaatg aatacaccga
      961 tgatgcgagc cgcactcgtc atttacgacc ctatcaggcg gtttgcgcta cctcaaactg
     1021 tttctgttcg ggggcgaaac cgtgggccgt gccgaagtaa ttgccccacg ccaggtcgat
     1081 cagctcgggc gtcaactgtc ccgggcgtcc ctcgaacaaa cagatgtctc tgactttgtt
     1141 aagcaggtcg cgcggttcgc atcctttcat gacgcgcttt tccgccgcgt acttttgcag
     1201 gacgtggtcg agacactctt gagtcacgac aatgccgcga cttgtggcag tgcgtttgaa
     1261 gatctccatg aacgcgcccg cggttggcgg ctcgacgcgc gcgcgatatc ccatgcggcg
     1321 cagaaacgct tcgtcaacaa gatctcgttc gtcgagatta gtagagaaaa tcactagctc
     1381 ttcgaacggc acttcaatct tcttgccgct gtgcaacgtc aggaaatcga ttcgcttttc
     1441 gaggggcaga atccagcggt ttagcaggtc ggtcggtgaa acgcgttgcc ggccgaagtc
     1501 gtcaatgacg agcgtgccgc cgttggcttt catctggaac ggcgcttcgt agaacttggc
     1561 tgactcggac cacgtcaaat ctgtattgtc cagcgtcaac tcgccggcaa caatcaccag
     1621 cggacgttga atctcgatcc cgcggcggtc gtgttcaatc aacgaatcct gttgcttaac
     1681 cgcgtggtga ttgtgcgcgt cgtaaacgcg gataatctgt ccatcgactt caaccgcgta
     1741 gggaatccag atggtgcctg acagcgccga gttcattcgt tccgacactg cggtttttcc
     1801 ggttcccgga agtccggtca ggaagagtga agagcgcgag ttgatcacgc aaccgagtgt
     1861 ttgcagtaag gagtcgggca gcacgagatc tttgaaggcg gaacggacat tttccatcgt
     1921 ggcggcgcgt gaaggaacag cctggagccg catcatatgc gcgtagtcgt cgagtgacac
     1981 gggcgctggt cctaagtaac cgctctgctg gtttagccgc ctgacgcgtt cccagccgtg
     2041 gtccaacatc gcgtagcgag tggcgccgac ggcgctttga agcctcatct cgatcaactt
     2101 ctcacgatag aggtgataga gaatctcctc ggtgagagcg cgtggcaggt gcatccggtc
     2161 ggccacggag atggtggtcg gttcgggcaa catggctacg tgtttgaggg ccaggtcgca
     2221 gaggaaagct tcgtgtacgc cggtctccga cacttcttcc ggagtggggg ggcccatgct
     2281 ttcgatttca gcccggctca agatgctcag atgtcccatc gaggtcactc ccgtttaagg
     2341 ttgccgctaa cttatggttc ttactgccgg cctgctgccg cagccgttgc cttcagtgat
     2401 aag
//
LOCUS       DASTEY010000056         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1204269, whole genome shotgun sequence.
ACCESSION   DASTEY010000056 DASTEY010000000
VERSION     DASTEY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204269"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFO72_01000"
     CDS             complement(<1..227)
                     /locus_tag="VFO72_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET9477888.1"
                     /translation="MKYVIERFRELHTSGCFVLPNPWDVGSAVYLRHLGFKALATTSS
                     GFAFSRGMPDDVRYVRRDTVLKHFREIVDATP"
     gene            265..1047
                     /locus_tag="VFO72_01005"
     CDS             265..1047
                     /locus_tag="VFO72_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9477889.1"
                     /translation="MRKVGALLSWFLVLASTAMAQTAPDAAELTKLLNDFLAGASRND
                     PAVHERFWANDLIYTRSAGRRVRKAEILRDLRSAPAPKPDDPKTVYTAEDIRIQQYGD
                     TAIVAFRLVGTTEVGGAKQVQNLLNSGTFVKRNGKWQVVNWQSTRMPRTEEENRADVA
                     AVTTAFREALRSNDLKKFAALTDPSFVWTEGSTRETTRQQIIETRSAITLKGVKSERP
                     RELISLYGETAVVRGQYSDGKYTMTLVNQGGAWKLVAVHSSN"
     gene            complement(1051..1866)
                     /locus_tag="VFO72_01010"
     CDS             complement(1051..1866)
                     /locus_tag="VFO72_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477890.1"
                     /translation="MPKEGSVMMPVRFRNYAKILVSLCLVLTFCLIEGEAQTRRKRTR
                     RAPKPAAPKPVITNPPIAPPTTTTPETAATGDVKIISTADSTTEEPTEPTQAKKPKTD
                     TTTTPSNEEIQETITNLSNQVNRLADKLGQMHDDDRYQLDMERLTRAEQRAEQLRMQL
                     VDVQSKIADMEARLEGVEFALRPENIERATATFGSTRPEEPRAARQRQLESERTRLKS
                     QLTILQTSEVRLAVSCANADNEVDLLRAKLEQRRAQMDATPPASESKPKKPKQ"
     gene            complement(1954..>2211)
                     /locus_tag="VFO72_01015"
     CDS             complement(1954..>2211)
                     /locus_tag="VFO72_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477891.1"
                     /translation="AGVHSGWRALGVPYEIGPFADHTTHLTLTAASRLFDDLPLKILS
                     KKSNIDEARARARKQPARHMGDRLKRVFFKNALYEVVARRT"
BASE COUNT          472 a          605 c          629 g          505 t
ORIGIN      
        1 ggcgtagcgt ctacaatttc acgaaagtgc ttgagcaccg tgtcgcgacg aacgtagcga
       61 acgtcgtcgg gcatgccgcg actgaatgcg aaaccggatg acgttgtggc gagtgctttg
      121 aaaccgaggt gacgaagata aactgcgctg ccaacatccc aggggttcgg tagtacaaag
      181 caacccgaag tgtgtagttc tctgaaccgt tcgataacgt atttcacgat cagattttac
      241 ccacggattc acagaaagga aactatgagg aaagttggag cactactgag ctggttcttg
      301 gtgctggcgt caacggcgat ggcccagacc gcaccagatg cagccgagtt gacgaaattg
      361 ctgaacgact tcttggcggg tgcatcgcga aacgatccgg ctgtgcacga acgtttctgg
      421 gctaacgatc tcatctacac gcgttcggcg ggccgtcgtg ttcgcaaggc cgagatcctg
      481 cgcgatctgc gttccgctcc cgcgccgaaa ccagacgatc cgaaaaccgt ctacaccgcg
      541 gaagacattc gcattcagca gtacggcgac actgcaatcg ttgctttccg tctcgtcgga
      601 accacagaag tgggtggcgc aaagcaagtg cagaacctct tgaatagcgg aacgttcgtc
      661 aagcgcaatg gcaagtggca agtagtgaat tggcagtcga cgcgtatgcc gcgcacggaa
      721 gaggagaaca gagcggacgt ggccgcggtc accactgctt ttcgcgaggc tttacgaagc
      781 aacgacctca aaaagttcgc tgcactgacc gatcccagtt ttgtttggac ggaaggctca
      841 acgagagaaa ccactcggca acaaataatt gaaacccgga gcgcgataac cttgaagggg
      901 gtcaaatcag agagaccgag agaacttatc tcgctttatg gtgagactgc ggtcgttcga
      961 ggtcaatatt cagacggcaa gtacacgatg acattggtta accagggtgg ggcctggaaa
     1021 ctcgtagccg tacacagcag caactagagt ttattgtttg ggcttctttg gtttggactc
     1081 acttgcgggc ggcgtggcgt ccatctgggc gcggcgctgc tcgagcttcg cacgcagcag
     1141 gtccacctcg ttatcggcat tcgcacacga cactgccaac cgcacctcgc tggtctgcaa
     1201 aatcgtcaac tgcgatttca accgagtcct ttcactctcg agttgtcgct ggcgcgcagc
     1261 gcgcggttct tccggacgcg tcgaaccaaa agttgccgtt gcgcgttcga tgttctccgg
     1321 tctcaaagca aactcaacac cctcgagcct cgcttccata tccgcaatct tgctttgcac
     1381 gtcgaccaac tgcatgcgca actgctcggc acgttgttcg gcgcgcgtca ggcgctccat
     1441 gtcgagctga tagcgatcgt cgtcgtgcat ctgacccaac ttatcggcga gacggttgac
     1501 ctggttggaa aggttcgtga tcgtctcctg gatctcttcg tttgacggcg tcgtcgttgt
     1561 gtcagtttta ggcttcttcg cttgagttgg ttcggttggt tcctccgtgg tggaatcggc
     1621 cgtgctgatg atcttcacgt cgccagtggc ggcggtttcg ggcgttgtcg tcgtgggcgg
     1681 cgcgatcggc ggattcgtga tgacgggctt gggtgcagcc ggtttaggcg cacggcgcgt
     1741 tcgcttccgt ctggtctgtg cctcgccttc aattaaacag aaggtgagaa ccaggcaaag
     1801 gctaacaaga attttggcgt agtttcgaaa gcgaacaggc atcatcacgc tcccttcttt
     1861 cggcactaag ataccccgct atccagatgc catcaagctc caggcagatg cttcgcttcc
     1921 acgttgcccc gggtgcttcc gttccaaaat atgtcaggtc cgccttgcga caacttcgta
     1981 aagcgcattt ttgaagaaca cacgcttcag ccggtctccc atgtgtcgag ccggctgttt
     2041 gcgtgctcgc gcccgcgcct cgtcgatatt gctctttttg ctgaggatct ttaggggtaa
     2101 atcatcgaat agtctactgg ccgccgtcag agttaaatga gtcgtgtgat cggcaaacgg
     2161 gccgatttca taaggcacac ccaatgcgcg ccagcccgaa tgcactccgg c
//
LOCUS       DASTEY010000057         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_116483, whole genome shotgun sequence.
ACCESSION   DASTEY010000057 DASTEY010000000
VERSION     DASTEY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116483"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFO72_01020"
     CDS             complement(<1..781)
                     /locus_tag="VFO72_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9477892.1"
                     /translation="MPLAAAEVGRPANEFMPNRRIYLDYSATTPVDPRVADAMARALT
                     GVFGNPSSVHSFGQQARAAVDRARREVAALINAKPNEIIFTSGGTEANNLAIRGLCEA
                     SVAHGRHIVTSTIEHPSVRGVCNALEQHGWEVTQLPVYQNGIVKVDDVRDALRLDTVL
                     VTVMLANNEIGTIQPVTEIAALIRERRSAGQRHLFLHTDAVQAAGRLVLNVEELGCDL
                     LTLSAHKLYAPKGIGALYLRRGVRLAGQNIGGHQERERRAGT"
     gene            967..1962
                     /locus_tag="VFO72_01025"
     CDS             967..1962
                     /locus_tag="VFO72_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477893.1"
                     /translation="MKHSKLPLVVLLSICLSGLLSVVSASAQGQSTALERGYRTGYSD
                     GYTAGDKDVSINAPRDYQSKEDYQRADRSFNEAWGTIEDYRDGYQQGFEAGYDAGYER
                     QQFNSSLPAGLRRRGTTGAPGVDVNVNTPATPAPNDEENQTTDTQVTVRGALAIPRNT
                     ILALELLTPISTDASQRGDRIQARVVQPAEYAGYIVDGHISQVRRPGKVKGVAQLQLA
                     FDQIRSTDNRTAELHAELMEISPMGAENEPQVDSEGGVRGRDSRKDDAAKVGAASGVG
                     AIIGEIAGGGKGAAIGAIIGGGAGTAGVMTQRGKDIRLERGQQLKIRTSTDTSVL"
     gene            1959..>2603
                     /locus_tag="VFO72_01030"
     CDS             1959..>2603
                     /locus_tag="VFO72_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9477894.1"
                     /translation="MTVITLLTDFGTADYFVPVVKGVILTSNPRVSIIDITHEIPPQD
                     IASGAFTLLTCYRDLPPGTIHVAVVDPGVGSSRRPIVVSAGSHCFVGPDNGLFSYVYD
                     NEPSHQTFHVTADEYFRGPVSPSFHGRDVFAPVAAALSKGLQPPALGPQITDEMRLAP
                     LSPIQEKNGTVRGRIIHIDRFGNCITNFRLADLPEQPELLVNGQVIRTVRTFFTD"
BASE COUNT          551 a          738 c          766 g          548 t
ORIGIN      
        1 ctgtacccgc gcggcgctca cgttcctggt ggccgccgat gttctggccg gcgagacgca
       61 cgccgcgacg aagataaagc gccccgattc ctttcggcgc gtacaacttg tgcgccgaca
      121 acgtcagtag atcgcagccg agctcttcaa cgttcagaac tagtcgtcct gctgcctgca
      181 ccgcatccgt gtgcaggaac agatgtcgtt gaccggcgga acgtctctca cggatgagtg
      241 cggcgatctc cgttaccggc tggattgtgc cgatctcatt gttggcgagc atgacggtta
      301 cgaggacggt atcgagccgg agtgcgtcgc gcacgtcatc gactttcacg atcccgttct
      361 ggtaaacagg caattgcgtg acttcccagc cgtgctgctc cagtgcgttg cacacaccac
      421 gaaccgaagg atgttcgatt gtagaggtga cgatatgtcg accatgcgcg accgaagcct
      481 cacacaagcc gcggatggca agattgttgg cttcagtacc gccggaagtg aagatgatct
      541 cgtttggctt cgcgttgatg agcgccgcaa cttcgcggcg ggcgcgatcc accgcggcac
      601 gcgcctgttg gccaaagcta tgtacgctcg acggattgcc aaatactcct gtcagtgcac
      661 gggccatcgc gtcagccacg cgcgggtcga cgggcgtagt ggcgctgtaa tccagataaa
      721 tcctgcggtt cggcataaac tcattcgctg gccgcccaac ctctgcggca gcaagtggca
      781 tctgaagggc agttctcggc ggcgctacga gtctaggccg cgcactatgg agcgtcaagc
      841 gactaatttg aattcttctc tgtgtaaatc cgcgtattcg gtgatagcat gcgcacgttg
      901 cgaggttggc tctcccaaga cgatacgcct ttgtggaggt cgtaaattct tcggaaggac
      961 ataaccatga aacattcaaa gcttcccctg gtagttttat tgagcatctg ccttagcgga
     1021 ctgctttcag tggtttccgc atctgcgcag ggacaatcga ccgcgctgga acgcggttat
     1081 cgcactggat attcagacgg ctacacggcc ggcgacaagg acgtttccat caacgcgccg
     1141 cgtgactatc aaagcaaaga ggattaccag cgggcggatc ggtctttcaa cgaagcctgg
     1201 ggaaccatcg aggactatcg cgacggttat caacagggtt tcgaagcggg ctacgacgcg
     1261 ggttacgaac ggcaacagtt caactcgtcg ctcccggccg ggttgcgaag acgtggaacg
     1321 actggcgcgc ctggtgtcga cgttaatgtc aacacgccgg caactcccgc tcctaacgac
     1381 gaagaaaacc aaacaacaga tactcaagta acggtcagag gggcgctggc gattccgcgc
     1441 aacaccattc tcgcgctcga actgcttact ccgatttcca ccgacgccag ccagcgcggc
     1501 gataggatcc aggcgcgtgt tgtgcagcct gccgagtacg ccggatatat cgtcgacggt
     1561 cacatctcac aagtgaggcg acccggcaaa gtgaaaggtg tcgctcaact ccaacttgct
     1621 ttcgatcaga ttcgctcgac cgataatcgc actgcggagc ttcatgccga gttgatggag
     1681 atatcgccga tgggcgctga gaacgaaccg caggtcgact cggaaggtgg agtgagaggt
     1741 cgtgattcgc ggaaagacga cgccgcgaaa gtcggtgctg cgtccggcgt cggcgcgatc
     1801 atcggcgaga ttgccggcgg aggtaaaggc gccgcgattg gtgcgatcat cggcggcggc
     1861 gcaggtacgg ccggtgtaat gactcaacgc ggcaaagaca tccggctgga acgcgggcag
     1921 caattgaaga ttcgcacttc aactgacacg agcgttctgt gaccgttatc actctgctta
     1981 cggattttgg gaccgcggat tacttcgtcc ctgttgtgaa gggtgtcatt ctcacgagta
     2041 atccgcgggt ttcgataatt gacatcaccc acgagattcc gccgcaggat atagcgtccg
     2101 gggccttcac cttactaacc tgttatcgcg acttgccgcc gggaacgatt cacgtcgctg
     2161 tggttgatcc cggtgtcgga tcgtcacgcc ggccgatcgt cgtgagcgct ggatcgcact
     2221 gcttcgttgg accagacaac gggctcttca gttacgttta cgataacgaa ccctcgcacc
     2281 agacgtttca cgttacggca gatgaatatt ttcgggggcc ggtgagtccg tcttttcacg
     2341 ggcgagacgt ttttgcgccc gttgccgcag cgctttcgaa aggccttcaa ccgccggccc
     2401 ttggaccaca gattacagac gaaatgcgat tagcgccgct gtcgccgatt caggaaaaga
     2461 atggcacagt cagagggcgc ataattcata tcgatcgttt cggcaactgc atcacaaact
     2521 tcagacttgc ggatctgccg gagcagccgg agttgctggt gaacgggcaa gtgatccgta
     2581 ctgtccgcac ctttttcacg gac
//
LOCUS       DASTEY010000058         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1435120, whole genome shotgun sequence.
ACCESSION   DASTEY010000058 DASTEY010000000
VERSION     DASTEY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435120"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFO72_01035"
     CDS             <1..468
                     /locus_tag="VFO72_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477895.1"
                     /translation="RGRTPETPPLPNPYRLTGRRDVIIKAVQDLMRDRKLVVDEAASK
                     FSDGIIVSQPFTFTKGAVVSEAELSRYADAPTSASRAWTRGRYTLTVEVQPIDGNSAN
                     VSVNAKVEGRTDGATGAEWTTLRSTGLAEDQFLEGLIVAMTGAAVPGRPLPEP"
     gene            490..1098
                     /locus_tag="VFO72_01040"
     CDS             490..1098
                     /locus_tag="VFO72_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477896.1"
                     /translation="MFNDPFHPEEAAIQQHDSAKRKLLAGVCAVAITAILLAGYAYIR
                     KYHAQRILANAPPTPEAVAKGPALAHVVIDEPTLEKDLTTVGGVVKNISNQPLSGLSV
                     TLELRRRKDGKTEQALLPVTPAQLQPQEEGAYAAKFSIEDFASVRLTGVHADPESTLI
                     VHTSSQGKKRAPERLQPQVINGRRSGKPGEFINTPNNPGRVP"
     gene            1400..1894
                     /locus_tag="VFO72_01045"
     CDS             1400..1894
                     /locus_tag="VFO72_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477897.1"
                     /translation="MAQNTQPERTPRPPRPQSPIPNRDETPLGSFEEELRAKRAIKLA
                     EKYHKENLDRAREVAELAKQLQTNLKDKKTIDRNSTKKLERLEKLTKKIRSEAGGEKE
                     EVEIVDRPANITAAVNQIADSAESLSKAVQDTPRQVVSASVIGNANVLLELIKLLRSF
                     DPRP"
     gene            1899..2906
                     /locus_tag="VFO72_01050"
     CDS             1899..2906
                     /locus_tag="VFO72_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9477898.1"
                     /translation="MARYSWNRGRLARTDSLVAPTVSSERDAGGFRVSPFLLGTFPFL
                     LVLLLITAVTTRAQDQDVIRTTTENVVLNVTVTDKAGQYVKGLKVSDFKVYEDGVEVK
                     PEMIANFSVHESPYAAVVLLDSSGSMEARFTLARSAAIQFLDRLREEDVAAVYRFDSK
                     VERVQEFSGGRDLAPIAYAIRAKGMTSLNDAIVEAARTLAERSENRKAIVILSDGMDT
                     FSKASSEKAVESALAIGASIYAVDMSDMKIGGSGSRQGAPSLKGFAEKTGGRFVATPG
                     GQALREAFAGIAEELGHQYTIAYRPLNRNRDGKWRKLDVKLDREDLTVRTRKGYRAPK
                     N"
     gene            complement(2911..4044)
                     /locus_tag="VFO72_01055"
     CDS             complement(2911..4044)
                     /locus_tag="VFO72_01055"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET9477899.1"
                     /translation="MPKKLRVGVIFGGRSGEHEISIRSARAIVESIDRKKFEVIPIAI
                     TKEGKWLPPAHSAQLLPAAVQPLLASSKTGPTGDVAILGDPSHKGLISLEGNDQPLVA
                     EKLDVVFPALHGTYGEDGTLQGLLEMADVPYVGCGVLASACGMDKVTMKSLFVQAGLP
                     ICKHVWFLRSQWRAEPAKIIRKVMRDIGVPCFVKPANLGSSVGVSRANDRQSLSEAID
                     LAAEYDRKIIVEEEVVAREIECAVLGNDQPKASLPGEYVVYKETARFLDYTEKYANTG
                     HVSFVVPAPLSKAMTARIQRMAIRAFQSIDGAGLARVDFFVTRDGGELIINELNTMPG
                     LTEVSGYPKMWEASGLTFVRLLETLIDLAFERHRDKSLTKTSR"
     gene            complement(4063..>4563)
                     /locus_tag="VFO72_01060"
     CDS             complement(4063..>4563)
                     /locus_tag="VFO72_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET9477900.1"
                     /translation="PSGNIHGMPLAALLGFGAPELTRVAGFSPKIDPALCAHVGARDI
                     DPGEKQLIQKLGVRFFTMREIDERGMHACMDDAIAIASKGTAGYAVTFDVDALDPGDA
                     PGSGTLVRGGLTYREAHLGMEKIAEHGRMRMLEIVEINTALDVNNKTAELGVELILSA
                     LGKTIL"
BASE COUNT         1065 a         1303 c         1256 g          939 t
ORIGIN      
        1 cgtggccgca cacccgagac gccgccactg ccaaatccct atcgactcac tggccgtcgt
       61 gatgtgatca tcaaagccgt ccaggatctg atgcgcgatc gaaagttggt cgtcgatgaa
      121 gccgcgtcaa agttctcaga tggaatcatc gtcagtcagc ccttcacctt tactaaggga
      181 gcagttgtgt cagaggcgga acttagccga tacgctgacg cgccgacttc agcttcgcgc
      241 gcgtggacgc gcggccgcta cacgttgact gtcgaagtcc agcccatcga cggcaacagc
      301 gcgaacgttt ccgtcaacgc gaaagttgaa ggccggaccg acggagcaac cggcgctgaa
      361 tggaccacct taagaagcac gggtctcgct gaagatcaat ttctcgaggg cctcatcgtg
      421 gcgatgacgg gcgcagctgt tccgggacga cctctgccgg agccttaaga aatcacttga
      481 agatctgaaa tgttcaacga tccatttcac ccggaagaag ccgctatcca acaacacgac
      541 tccgccaagc gcaaactcct cgccggtgtt tgcgcagtgg cgatcactgc aatcctgctc
      601 gccgggtatg cctatatccg gaaataccac gcgcaacgaa ttcttgcgaa cgcgccgcca
      661 actccagagg ccgttgccaa aggaccagcg ttggcccacg tcgtgatcga tgagccgacg
      721 ctcgagaaag acctgacgac ggttggtggt gtcgtgaaga acatctcgaa ccagccgctt
      781 tccggccttt ctgtaacgtt ggaattgcgc aggcgaaagg acggcaagac cgaacaggct
      841 ttgttgccgg taacacccgc tcaactgcaa ccgcaggaag agggcgcata cgcggcgaaa
      901 ttctcgatcg aggattttgc gtcggttcga ctcaccggcg tgcacgcgga tccggagtcg
      961 acgttgattg ttcacacctc ctctcaaggc aagaagcgag cgcctgagcg actccagccg
     1021 caagtaatca acggccgacg gtctggcaag cccggcgagt ttattaacac gccgaacaat
     1081 cctggtcgcg tcccttaacc taattcctga attgtgtgtg tgtccgtgca atccgcggct
     1141 cacgtttctg ttgaagggtc ggcgtgccga tggttaaggt gaaccctcag ataacgtgcg
     1201 gcgtgcaacg ctgcgggttc gcgagccgtc acagtggttg agcgaattta gacggttgcg
     1261 ttagcattca cgtcaggcct ttgctcctcg gggtcaacac gaaaatcgaa tgaagaaaac
     1321 aacgccatac agagtgcacg cctgcgctcg cttcatcgtc ttcgggtgtg cccttctcgt
     1381 tctaagcgca agcatcgcag tggctcaaaa cacgcagccg gaaagaacgc cgcgtcctcc
     1441 gcgtcctcaa agcccgattc ctaatagaga cgaaacgcca cttggcagtt ttgaagaaga
     1501 actacgcgcc aaacgcgcaa taaagttggc cgaaaagtat cacaaggaaa acttggatcg
     1561 cgcgcgcgaa gttgctgaac tcgcaaagca actgcaaacg aacctcaaag acaagaagac
     1621 gatcgatcgc aattcaacga agaagctcga acgcctcgaa aagttgacga agaagattcg
     1681 cagcgaggca ggcggcgaga aggaggaggt agaaatagtc gaccgcccgg cgaatatcac
     1741 cgcggccgtt aaccagattg ccgacagcgc cgagtcgctg tcgaaagcgg tgcaggacac
     1801 gccgcgccaa gttgtctccg cttcggtgat cggcaacgct aatgttttac ttgaactgat
     1861 caagttgctg cgtagcttcg atccgcgacc ttaatcagat ggcacgatat tcctggaacc
     1921 gcgggcgtct cgcccgcact gattcgttag tagccccaac cgtttcaagc gagcgagacg
     1981 ccggcggctt tagggtttcc cctttcttac tcgggacgtt tcctttcctg ctcgtgctct
     2041 tattgattac cgctgtaacc acgcgcgccc aggaccaaga cgtcattcgc accacgaccg
     2101 aaaatgtcgt gctgaacgtc accgttaccg acaaagcggg ccaatacgtt aaagggctca
     2161 aagtctccga cttcaaagtg tatgaagatg gagttgaagt aaaacccgag atgatcgcga
     2221 acttttcagt tcacgaatca ccttatgcag cagtcgtgtt gctcgacagt tcgggaagca
     2281 tggaggcgcg cttcaccctc gcgcgttcag cagcgatcca gtttcttgac cggctgcgcg
     2341 aagaagacgt cgccgctgtc tatcgtttcg attcaaaggt agaacgcgtt caggagttct
     2401 ccggcgggcg cgatctcgcg ccaatcgctt acgcgattcg cgccaagggt atgacttcac
     2461 tcaacgacgc aatcgtcgaa gctgcacgca cactcgccga acgatcagag aatcgcaaag
     2521 caatcgtcat cctttcagac ggcatggaca ctttcagcaa agcttcaagt gaaaaagcag
     2581 tcgagagtgc actcgcgatc ggcgcaagta tctacgccgt cgatatgtca gatatgaaaa
     2641 tcggtggatc gggctcgcga cagggcgcac cgtcgttgaa aggttttgcc gagaagaccg
     2701 gcggcagatt tgtcgcgaca ccgggcggcc aggccttgcg cgaagcattc gccggtattg
     2761 cggaagagtt gggccaccag tacacgattg cttaccggcc gctaaacaga aatcgcgatg
     2821 ggaagtggcg aaagttggat gtgaaactgg atcgagagga tctcacggtg cggacccgca
     2881 agggttatcg cgcgcctaag aactaaaagc tcacctactg gtcttcgtga gtgacttatc
     2941 acgatgccgt tcgaaggcta ggtcgatcag agtttcgagc aagcgaacaa acgtaaggcc
     3001 ggaagcctcc cacatctttg gataacccga aacctcagta aggccgggca tcgtattcaa
     3061 ctcgttaata atcagctcac cgccgtctcg cgtcacaaag aagtcgactc gcgctagtcc
     3121 cgcgccgtcg atcgattgaa acgcgcggat agccattcgt tgaatgcgcg ccgtcatcgc
     3181 cttcgacaaa ggagcgggaa cgacaaaact gacgtggccc gtgttcgcat acttctctgt
     3241 gtagtcgaga aaacgtgccg tttctttata gaccacgtac tcgccgggca aactcgcctt
     3301 cggctgatcg ttacccaaca cggcgcactc aatctcacgc gctacaacct cttcttcgac
     3361 gatgatcttg cgatcgtatt ccgctgccag gtcgatagcc tcactaagtg attgcctgtc
     3421 gttggctcgc gaaacaccaa ctgaggatcc aaggttggct ggcttaacga aacagggcac
     3481 accgatatca cgcatgactt tgcggatgat ttttgccggc tctgcgcgcc attgcgatcg
     3541 caggaaccag acgtgtttac aaatcggcaa accggcctga acaaacagcg acttcatcgt
     3601 caccttgtcc atcccgcaag cggatgcgag cacgccacag ccaacgtaag gaacgtccgc
     3661 catctcgagt aaaccttgaa gtgtgccgtc ctcgccgtat gtgccatgta acgcaggaaa
     3721 gacgacgtcg agtttttctg cgacgagtgg ttgatcgttg ccttcgagcg agatgagacc
     3781 tttgtgcgag gggtcgccaa gaatcgccac gtcgccggtt ggtcctgttt tggaagaagc
     3841 gagcaggggt tggacggcgg cgggcagcag ttgcgccgag tgtgccggcg gcagccactt
     3901 cccttccttt gtgatggcga tcggaatgac ctcaaatttt ttccgatcga tactttcaac
     3961 gatcgctcgc gccgagcgaa tggaaatttc gtgttcaccc gaccgcccgc cgaagatcac
     4021 tccgacgcga agcttctttg gcactcttgt tggtccaacc ttctacagaa ttgtcttgcc
     4081 taaagctgag aggatgagtt cgacacctaa ctcagcagtc ttgttattga cgtccagggc
     4141 cgtgtttatc tctacgattt cgagcatgcg catgcggccg tgttcggcga tcttttccat
     4201 gcccaggtgc gcttcacgat acgttagtcc tccgcgcacg agagtgcccg aaccgggggc
     4261 gtcgccggga tccagtgcgt caacgtcgaa cgttacggcg taaccggccg tcccttttga
     4321 agcgatggcg atcgcatcgt ccatgcaggc atgcatgccg cgctcatcga tctcccgcat
     4381 ggtgaaaaag cgaacgccga gtttctggat cagttgcttt tcaccgggat cgatgtcacg
     4441 cgccccgacg tgcgcgcaca acgccggatc gattttggga gaaaaaccag cgacccgagt
     4501 caattcgggt gcgccaaaac caagcaacgc ggcgagtggc attccatgaa tgtttccgga
     4561 cgg
//
LOCUS       DASTEY010000059         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_314335, whole genome shotgun sequence.
ACCESSION   DASTEY010000059 DASTEY010000000
VERSION     DASTEY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314335"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..2056
                     /locus_tag="VFO72_01065"
     CDS             245..2056
                     /locus_tag="VFO72_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9477901.1"
                     /translation="MRDLRRLLKYLKPHWPVFTVATFAMVLVGLLESATGALIVPIFD
                     QAFAPGAGQRTATLFGLQNLIPQEPLGAWKMISILLVVFAVAKGIAEYLSTYLMARIG
                     QSAVLKLRQDLYGHLLNQSATFFERHRTNYLVSRLVSSASAIEAGITATLRDMLRESF
                     ALIAFLGASFYYNWRLTLASLVIAPVIALLTAKFGKALRNLARETLEGTKELTDTAHE
                     ALANQGIVKAYRAEQWEANRFVAVAKRIMRANLRSASIAGVSPPTIELIGILFVVLLL
                     FFGQQEIMAGRMNTAQFLTFIFFLFRSYDPVRKLSRLQNSMEQALAAAHHVWEVLDEH
                     AEIKEKPNAGPLAPLQNEIELRNVSFGYANESRSVLRDVNLKIKAGTMVALVGESGGG
                     KSTLTKLLPRFHDPTSGAVLWDGVDLRDTTLASLRRQIALVTQETVLFNDTVRHNISY
                     GKPDATDIEIEEAALIAFAHDFIREMPDKYDTIVGERGIFLSGGQRQRLAIARAILVN
                     APVLILDEATSALDAESERLVQRAIANLVRNRTTIVIAHRLSTVRRADVIVVMEAGRI
                     IEMGTHTELLAKNGQYRKLYELQFADEEEEPQIAQMI"
BASE COUNT          468 a          591 c          574 g          482 t
ORIGIN      
        1 tcagtcccca tggttgtcgc aagttccaac ctctgggctg gaattcgata acgccttcgg
       61 cgttcagatt aggacaccac ccattcgcga gggaacccgt gtgagggatc atcttttgtt
      121 cagatacgag taaatcagcc agccttgacg caaattagcc ggggtaagta tagttccatt
      181 tcagattttg gaccttcttc tggccttcct tatagcaact gagcagagac tttcgcaacc
      241 agagatgcgt gatcttcgtc gcctgttaaa gtatctgaaa cctcactggc cggtttttac
      301 agtcgcgacg ttcgcaatgg ttctcgttgg cctgctcgaa agtgcaaccg gcgctctgat
      361 tgtaccaatt ttcgatcagg ctttcgcccc aggcgcggga caacgcacgg cgactctttt
      421 tggcctgcag aatttgatcc cacaggaacc gctcggcgcc tggaagatga tctcgattct
      481 gctcgtcgtg ttcgcggtcg cgaaaggaat tgcggagtac ctgtcaacct atttgatggc
      541 gcgaatcggg cagtcggcgg tgctcaagtt gcgccaagat ctttatggtc acttgttaaa
      601 ccagtcggct accttcttcg aacgccaccg gacaaactat ctcgtgtcac gactcgtttc
      661 cagcgcttcg gcgatcgaag cgggaattac ggctacgctt cgcgacatgc tgcgcgagag
      721 ttttgcttta atcgcttttc tcggagcatc cttttattac aactggcgct tgacgctcgc
      781 gtcgttggtg atcgcgcccg tcatcgcact gctcacagcg aagtttggca aggcgttacg
      841 caaccttgca cgcgaaactc ttgaaggaac taaagaacta actgacactg cccacgaggc
      901 actcgccaat cagggaatcg tcaaagcgta tcgcgcagag caatgggaag ccaatcgttt
      961 tgtcgcggta gcgaagcgca tcatgcgagc aaacctgcgc agcgcgagca ttgccggcgt
     1021 atcgccgccg acgatcgagt tgatcggaat tctcttcgtc gtgctgctgc tattctttgg
     1081 tcagcaagag atcatggcgg ggcggatgaa caccgcgcag tttctcacgt tcatcttctt
     1141 tcttttccgc agctacgatc cggtgcgcaa actctcgcgt ctgcaaaaca gcatggaaca
     1201 ggcgctcgcc gcagcgcatc acgtttggga ggtactggac gagcatgccg agatcaaaga
     1261 aaagcctaac gctggaccac ttgcgccact gcaaaacgag attgaattgc gcaacgtctc
     1321 ctttggctat gccaacgagt cgcgctcggt cttgcgcgac gtgaacttaa agatcaaagc
     1381 cggaaccatg gtcgcactgg tgggcgagtc aggcggcgga aagtcgacgt tgacgaagct
     1441 gttgccgcgt tttcacgatc cgacttccgg cgcagtgttg tgggacggag tcgacctgcg
     1501 agacacaacg ctcgcgagcc tgcgacgcca gatcgcttta gttacgcagg agacagtgct
     1561 ctttaacgac actgtccgtc acaacatctc gtacggtaaa cctgacgcta cggatatcga
     1621 gatcgaagaa gccgcactga tcgcgtttgc tcacgacttc attcgcgaaa tgcccgataa
     1681 gtacgatacg attgtcggcg agcgcggcat ctttctttcc ggcggtcaac gccaacgact
     1741 ggccatcgct cgtgccattc tggtaaatgc cccagttttg attttggacg aggcgacctc
     1801 cgcgctcgac gccgagtcgg aacgcctggt gcagcgggcc attgccaacc tcgtccgtaa
     1861 ccgtacgacg atcgtgatcg ctcaccgcct ctcaacggtg cgtcgcgcag acgtgatcgt
     1921 ggtcatggaa gcgggccgca tcatcgaaat gggtacccac accgagttgc tggcgaaaaa
     1981 cgggcagtat cgaaagctct acgaacttca attcgccgac gaagaagaag agccacagat
     2041 tgcacagatg atatgaaatc aatgaccgga tacggcaagg cgatggttgc gggtgacgac
     2101 ttctccgtct ccgtc
//
LOCUS       DASTEY010000060         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_83623, whole genome shotgun sequence.
ACCESSION   DASTEY010000060 DASTEY010000000
VERSION     DASTEY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83623"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2106)
                     /locus_tag="VFO72_01070"
     CDS             complement(<1..>2106)
                     /locus_tag="VFO72_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477902.1"
                     /translation="DGIPDDARTFVVVPTIFINEDQVHELIERLEVHFLANQDPHIGF
                     ALLGDFPDAPAEEMPNDSLLLSVAQSGIDWLNQKHGEGRFHLFHRRRQWNAGENKWMG
                     WERKRGKLEEFNRLLRGARDTSFVVRTADDALLRSIRYVITLDSDTQLPRDVARKLVG
                     AAEHPLNRPRFDESANRVTRGYGILQPRVSISLSSASRSKFVQIFSGYTGIDPYTTAV
                     SDVYQDFFGEGTFTGKGLYDVDAFQRALEDRVPENALLSHDLFECLFARAALTTDIEV
                     LDEYPASYEAYAKRLHRWTRGDWQIIRWLFPRVPHGSGKKVRNPLPLLARWKIFDNLR
                     RSLLAPSLFLWFVAACAIFPGSAWLWALFILIVIAFPVYLHVTTDLLTHPKGIPWSSH
                     FYSVWGQFRTNTAQIAFSFVFLPYQAYLMCDAIVRTLYRQLISKRKLLEWMSAAEAER
                     SIRYDFVSYLSLMLPAEILALAALGLTAVLKPSGLPLMGVLSAIWILSPLIALWASKP
                     RPAVRQVLEDDEKHFARLIARRTWRFFETFVGKEDNWLPPDNFQEDPAPVIAHRTSPT
                     NIGLLLLATSSAHELGYVGTLELVERQELTFATLAKLARLHGHYFNWYDTRTLEPLLP
                     QYISTVDSGNLAGHLIAVKQGSVEIPDNKLFGPRVIRGFTDTLDALAFEATSLGSFRQ
                     RTEVVTVRQLRDEIAACRE"
BASE COUNT          481 a          567 c          644 g          414 t
ORIGIN      
        1 aactcgcggc aggcggcaat ctcatcgcgt aattggcgaa cggtgacgac ctcggtgcgt
       61 tggcgaaaac tgcccagact cgtcgcttca aaggcaagcg catccagcgt gtcggtgaat
      121 cctcgaataa cgcgcggccc aaacaacttg ttgtccggta tttctaccga gccttgcttt
      181 actgcgataa ggtggccggc gaggttgccg ctatccacag tggaaatgta ttgcggcaag
      241 agcggctcga gagttctcgt gtcgtaccag ttgaagtaat gaccgtggag ccgggccaat
      301 tttgccagcg tggcgaaagt tagttcctgt cgttcaacca gttcgagcgt gccgacataa
      361 ccgagctcgt gggccgatga tgttgcaagc aatagcaggc cgatgtttgt gggcgacgtg
      421 cggtgtgcga tgacgggcgc cggatcttcc tgaaaattgt ctgggggtaa ccagttgtct
      481 tctttgccga cgaacgtctc gaagaagcgc caagtgcggc gggcgatcaa gcgggcaaag
      541 tgtttttcat cgtcctccag tacttgccgc acggctggac gcggtttgct ggcccaaagc
      601 gcgattaacg gcgaaaggat ccagatcgct gaaagcacgc ccatcagcgg aagtccggac
      661 ggtttcaaaa ccgctgtgag accaagcgcc gcgagcgcga ggatctcggc gggcagcatc
      721 agcgaaagat acgaaacaaa atcgtagcgt atcgaccttt cggcttcggc ggctgacatc
      781 cattcgagga gctttctctt cgagatcagt tggcggtaga gcgtgcgaac gatggcgtcg
      841 cacatcaaat aagcctgata aggaagaaag acaaacgaga acgcgatctg tgcggtgttc
      901 gtccgaaact ggccccagac gctatagaag tggctgctcc agggaatacc tttgggatgt
      961 gtgagcagat cggtcgtgac gtgcaggtaa accggaaagg cgatcacgat caagatgaag
     1021 agcgcccaga gccacgcgga acccggaaag atcgcgcacg ccgctacgaa ccagagaaag
     1081 agcgagggcg cgagcaagct gcggcgcagg ttgtcgaaaa tcttccaacg cgcaaggagt
     1141 ggcagaggat tgcggacttt cttaccgctc ccatgcggca cgcgtggaaa caaccaacga
     1201 ataatctgcc aatcgccgcg cgtccaacga tgcaggcgtt tagcatacgc ttcgtaagaa
     1261 gcaggatact cgtcgagcac ttcgatatcg gtcgtgagcg ccgcgcgcgc gaacaagcat
     1321 tcgaaaagat cgtgactcag cagtgcgttc tccggcacgc gatcctcgag tgcgcgttga
     1381 aaagcatcga cgtcgtacaa gcctttaccg gtgaaggtcc cctcgccgaa gaaatcctga
     1441 tacacatccg agactgcggt ggtgtacggg tcgatgccgg tgtaaccgga aaagatctga
     1501 acgaacttcg atcttgaagc actgctcagc gagatgctga cgcgcggttg cagaatgccg
     1561 tagccgcgcg taacgcgatt ggctgattcg tcaaaacgtg gccggttgag aggatgttcg
     1621 gcggcgccga ccagcttgcg agcgacgtcg cgcggcagtt gggtgtctga atcgagcgtg
     1681 atcacgtagc gaatagatcg cagcagcgcg tcgtcagcgg ttcgcactac gaaactggtg
     1741 tcgcgcgctc cgcgtagcag ccggttgaac tcttccaact taccgcgctt tcgttcccag
     1801 cccatccatt tgttctcgcc agcgttccac tgtcggcgcc ggtgaaacag atgaaagcgg
     1861 ccttcaccgt gtttttgatt cagccagtcg ataccgctct gggccacgga caggagcaga
     1921 gaatcgttag gcatctcttc cgccggggca tcggggaagt cgcccagcag cgcgaacccg
     1981 atgtgcggat cctgattggc gaggaagtgg acctcgagcc gttcgatcaa ctcgtggacc
     2041 tggtcttcgt ttatgaagat cgtgggaacg acgacaaacg ttcgcgcatc gtccggaata
     2101 ccgtca
//
LOCUS       DASTEY010000061         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_17616, whole genome shotgun sequence.
ACCESSION   DASTEY010000061 DASTEY010000000
VERSION     DASTEY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17616"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1479)
                     /gene="aceA"
                     /locus_tag="VFO72_01075"
     CDS             complement(163..1479)
                     /gene="aceA"
                     /locus_tag="VFO72_01075"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET9477903.1"
                     /translation="MKTDDHVALENEWTTSERWRGIKRGYTAADVVKLRGSVEIKHTL
                     AERGAKRLWELLHREPYVPALGAMTGCQAVQQVQAGLKAIYLSGWQVAADANLAGHMY
                     PDQSLYPANSVPEVVRKINQVLQRADQIDHAEGRNGIDWFVPIVADAEAGFGGNLNAF
                     ELTKAMIEAGAAAVHFEDQLASAKKCGHMGGKVLVPTQEFIQKLVAARLAADVMGVDT
                     ILVARTDADAAGLITSDIDERDHQFLTGNRTPEGFFTVKPGLDQAIARALSYAPYADV
                     VWCETSEPNLEDASRFAHAIHSQYPGKLMAYNCSPSFNWKKKLDDRTIAKFQKELGLM
                     GYKFQFITLAGFHALNLAMFELAQEYRESGMSAYAKLQATEFAREADSNYRAVKHQSF
                     VGTGYFDAIAQVIAGGNSSTTALTGSTEEAQFTQQIVREASTALSV"
     gene            complement(1506..3041)
                     /locus_tag="VFO72_01080"
     CDS             complement(1506..3041)
                     /locus_tag="VFO72_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477904.1"
                     /translation="MSSTRSQYGTRFNTAPPEFNPETDLPEGFLAFLLPLHQELSPRQ
                     PSLIQKRATVLTSAHQGQKPNYLPPSEATTTGWTIPLPQWCADQRNQMTGPADDAELV
                     VKMLNSGAPGVMLDLEDSMANTWANLTTGLANIDAALKGELTYFDAKRDRRAGINDSN
                     TVTFIRPRGLHISQAGVLNDELMSASLFDVAMVAYKADVDRLRHPLSFYIPKSESAEE
                     ALWWRDLFRTIEDARGWARGYIKCMALVESHPIAYQMEEFIYNLRDHIVGLNLGRWDY
                     MASLIHFNLEDPEWVLPDRNTIPHDVPFFQNLRELLAETCHKHGILAIGGMTALYPSR
                     TDPELNERALRVLAADKKNEANCLMDGAWTGHPDQNEIAVQQFPFPNQVNARKSSAET
                     HPDLRPSPRGVGSITANGTRAAIRTVIRYRNGVLNGKGASLLDGYMEDLATDRIYRLM
                     IAQRIRHGDNTPESISHMFDEELERILTELPAEAGEPVIEKYRTARQISELLIRNQEF
                     DPI"
     gene            3247..4056
                     /locus_tag="VFO72_01085"
     CDS             3247..4056
                     /locus_tag="VFO72_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET9477905.1"
                     /translation="MATARNQDSAKPDHNREQVTSVERALSILELLASRNHGLSTSEI
                     SRVAKVPKSSTSYLLRTLVSRGYVRRDVETGQHTLGIRILSLGGQAMQGMALRELAMP
                     HLRQIVESTRLGSHLAILDHGDAVYIERIESPGFIKMDIWNGRRVAPQATAVGKALIC
                     HLSREEVQEIAGLHPLRQVSPRTIVTLPHLFEELTAIRRRGYAIDDEEHAIGVRCVAA
                     PVFSASGEVVAAIGLSGTVSQLNDDYLPSLGKIVQTAALKLSAQLGGRRSR"
BASE COUNT          829 a         1117 c         1205 g          946 t
ORIGIN      
        1 ctggggcgta agccccaggt aaattgttaa aaagaacgtg tgagcctgcg aagcgggcga
       61 cagcgctgcc gcccgtttca cgggctcaat ccgtttttct ttcttcgatc ctggggctcg
      121 cgccccaggc tttattcggc cacctgcttc gcaggttccg ggtcaaacac taagtgctgt
      181 tgaagcttcg cggacaatct gttgagtaaa ttgtgcctcc tcggtcgagc cggtgagcgc
      241 ggtcgtcgag gaattgccgc ctgcgattac ttgtgcgatc gcgtcgaagt agccggtgcc
      301 gacaaacgac tggtgcttga cagcgcgata gttgctgtcg gcttcgcgcg cgaattcggt
      361 tgcctggagc ttcgcgtagg cgctcatgcc cgattcgcga tattcctgcg ccagctcgaa
      421 catcgcgagg ttgagtgcgt ggaagcccgc gagcgtgatg aactgaaatt tgtaacccat
      481 caacccgagc tctttctgaa acttcgcgat cgtgcggtcg tcgagttttt tcttccagtt
      541 gaacgacggc gaacagttgt aagccatcag cttgcctgga tactgcgagt ggatcgcatg
      601 cgcaaaccgg ctcgcatctt cgaggtttgg ctcggaagtc tcgcaccaga cgacgtctgc
      661 gtaaggcgcg taagacaagg cgcgcgcgat agcctggtcc aacccgggtt tgaccgtgaa
      721 gaaaccttcc ggagttcggt tgccggtcag gaattgatga tcgcgttcgt cgatgtcgct
      781 ggtgattagt ccagcggcgt ctgcatcggt gcgcgcgact agaatcgtgt cgacgcccat
      841 cacgtcggcc gccagccttg ccgcgaccag cttttgaata aactcctgcg tcggtaccag
      901 caccttgcca cccatgtgac cacacttctt ggccgacgcg agctgatctt caaaatgtac
      961 ggccgcagct ccggcttcga tcatcgcttt cgtcagctcg aacgcattca agttgccgcc
     1021 aaaaccggcc tcagcgtcag ccacgatcgg aacaaaccaa tcgatcccgt tccgaccttc
     1081 agcatgatcg atctgatcgg cgcgttgcag cacctgatta atcttgcgca cgacctcggg
     1141 aacgctgttc gctggataca gactctgatc gggatacatg tgaccggcga gatttgcgtc
     1201 agccgcgacc tgccaaccac tcaggtagat tgccttcaaa cccgcctgca cttgttgcac
     1261 cgcctgacag ccagtcatcg ctccaagcgc ggggacatat ggctctctgt gcagcagttc
     1321 ccagagacgt tttgcgccac gctcggcaag cgtgtgtttg atctcgacag acccgcgtag
     1381 ttttacaacg tccgccgcgg tgtaaccgcg cttgattccg cgccagcgct cagacgtggt
     1441 ccattcattt tccagtgcta catgatcgtc ggttttcatg agtatccctc ctggttttgt
     1501 gaacatcaga tcggatcaaa ttcctgattc cgaattagca attcgctgat ctggcgcgcg
     1561 gtgcgatatt tctcaatcac cggctcgccg gcttcagcgg gcagttcagt caagattcgc
     1621 tcgagctcct cgtcgaacat gtggctgatc gactcgggcg tgttgtcgcc atgacgtatg
     1681 cgttgcgcga tcatcaagcg ataaatgcga tcggttgcga gatcttccat gtagccgtcg
     1741 agcaagctgg cgcctttgcc gttcagcacc ccatttcgat aacgaatgac agtgcggatt
     1801 gcggcgcgcg ttccgtttgc agtaatgctg ccgactcctc tcgggctggg ccgcagatcc
     1861 gggtgggtct ccgctgacga tttgcgtgcg ttaacctggt ttggaaacgg aaactgctgc
     1921 actgcgatct cgttctggtc tggatgtccg gtccatgcgc catccatcaa acagttcgcc
     1981 tcgttcttct tgtccgcggc gagtacgcgc agcgctcgtt cgttcaattc gggatcggta
     2041 cggctgggat acaacgccgt catgccgccg atggcgagga tgccgtgttt gtggcaagtc
     2101 tcggccagca actcgcgcag gttttgaaag aacggtacgt catgcggaat cgtgtttcga
     2161 tcgggcagga cccactcggg atcttcgaga ttgaaatgga tcaagctggc catgtaatcc
     2221 cagcggccaa gattcaaacc gacgatgtgg tcgcgcaggt tgtagatgaa ctcctccatc
     2281 tgataagcga tggggtgcga ctcaactaac gccatacact tgatgtaacc acgcgcccat
     2341 cctctggcgt cttcgatcgt gcggaataga tcgcgccacc acagcgcttc ttccgccgac
     2401 tcagatttcg gaatgtagaa gctgagcgga tgtcgcagac gatcgacatc agctttgtaa
     2461 gccaccatcg ctacgtcgaa cagtgaggct gacatcaact cgtcgttgag tactccggcc
     2521 tggcttatgt gcaagccacg cgggcgaatg aaggtgactg tgttgctgtc gttgatgcct
     2581 gcacggcgat cgcgtttcgc gtcgaaatag gtgagctcac ctttcagggc cgcgtcgata
     2641 ttcgcgaggc cggtggtcag gttagcccac gtgttggcca tcgagtcttc caggtccagc
     2701 atcacgccgg gcgcgcccga gttcagcatt ttcacgacga gctcagcatc gtccgccggt
     2761 ccggtcatct ggttgcgctg gtcggcgcac cactgcggca gcggaatcgt ccagccggtg
     2821 gtggtggctt ccgaaggcgg cagatagttt ggcttctgac cctggtgtgc ggaggtgagg
     2881 acggtcgcac gcttttgaat gagggatggt tgccggggtg atagttcctg atgaagtggt
     2941 aatagaaacg ccaggaatcc ttcgggaaga tcggtctccg ggttgaactc cggtggcgcg
     3001 gtattgaaac gtgttccgta ttgtgaacga gtgctcgaca taatgtgagg agaggtttag
     3061 catcagtttt cacaacctgc aagacggcaa tcgcaatggt gaacgcattg ttgtgtacgt
     3121 tggacaccca gaatgtccgt tttagcagct ttatttgaaa aagaagccga attgtcttta
     3181 taggtcaaat caggcgtgtt caatatcgtg aacaaaattt aagagtgtgg aggcaggatg
     3241 gcgcgagtgg caacagcaag aaatcaggat tcagcgaagc cggatcacaa tcgtgaacag
     3301 gtgacttcgg tagaacgtgc tctctcgatt ctcgagctgc tggccagcag gaatcacggg
     3361 ctcagcacgt ccgagatcag ccgtgtagct aaagtgccga aaagctcgac cagttatctc
     3421 ttgcgaacgc tggtgagtcg tggctacgtg cgacgcgacg tcgagaccgg gcagcacaca
     3481 cttggaatac gaatcctcag tttgggtgga caggcgatgc agggcatggc gttgcgtgag
     3541 ttagctatgc cgcacctgcg ccagatcgtt gagtccacgc gtttgggttc gcatcttgcc
     3601 attctcgatc acggtgatgc ggtgtacatc gagcgaatcg agtcgccggg ttttatcaag
     3661 atggatatct ggaacgggag acgtgtagcg ccgcaggcga cagcggttgg caaggcgctg
     3721 atctgtcact tgagtcgtga agaagtgcag gagattgcgg ggctgcatcc attgcgtcag
     3781 gtatcgccga gaacgatcgt cacgttaccg cacctgttcg aagaactcac tgcgatacgc
     3841 agacgcgggt atgcgattga cgatgaagaa catgcgatcg gcgtgcgttg tgttgccgct
     3901 ccggtatttt cagcgagtgg tgaagtagta gcggcgattg ggttaagtgg aacggtcagt
     3961 cagctcaatg acgactacct gccgtcattg ggcaagatcg tccagaccgc cgcactaaaa
     4021 ctctcggccc aactcggcgg ccgccgcagc cgttaaccac tgatccacag caacccctca
     4081 cgggcgacgt cgttggg
//
LOCUS       DASTEY010000062         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_528629, whole genome shotgun sequence.
ACCESSION   DASTEY010000062 DASTEY010000000
VERSION     DASTEY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528629"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2559
                     /locus_tag="VFO72_01090"
     CDS             <1..>2559
                     /locus_tag="VFO72_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9477906.1"
                     /translation="AGLRADGGAAFSVELESEERLVVVHEIQTRCTTEASEIIAAIRA
                     VIAEEFEIQPAAVVLIRPGTLPKTSSGKVRRGACRERFLENRLRVIAEWRATGERNGF
                     TPAAPEELNAETIERWLRLLLSARLSLDGPLIDARQPIARYGIDSLLALELAHAVETT
                     LGIRLSPASLLRNPIVELTNEILVQLARTEHSHAVAHVATVDQSGEHSLSHGQQALWT
                     LQQASPESTAYNVSAAARVRGEVDTRALQRAFRILSRRHSMLRARFPSRQGAPVCAIQ
                     EHSAISFRIEDAMSWSEEKLNNRLRDEAWTSFDLERGPLLRAGLFKRSEREYVMLLSA
                     HHIIVDFWSLAVLIRELGQLYEAEVEDRPLDLAAVRAYSDYVRRQTELLASSEGERSR
                     KYWLQQLAGELPVLDLPADRARPPIQSYRGASVSARLDQQLTEQLKKLAMSHEATLFM
                     TLLAAFQVLLYRYTGQDEILIGSPSSGRGWAEFAGTVGYFVNPLVLRAQISSERSFSD
                     FLTETRQTALGAFEHQDYPFNLVVKQLQPERDPARSPLFQVMFALQKGEAALKLGGLP
                     VEPIALDHRAAQFELSLTVVEVDSGLTASFEYNTDLFDALTIERLSGHFRILLESVVT
                     DPSVSLSELPLLSDATRQQILYDWNHTQTRVSPVVCVHESFAQQAASSPDRIAIDSHE
                     ESLSYRELNKRANRMAHYLRGRGVGPEVLVGICVPRSAAMLTCVLGVLKAGGAYVPLD
                     PSYPEERLAFMLEDSGVSLLLTEQLLEVDGPAIDAESDADPIPSATFDHLAYVIYTSG
                     STGMPKGVMVQHGSLANYVHAANVVYGVGPEDRVLQFSSFSFDAHAEEIFVSLTS"
BASE COUNT          492 a          709 c          782 g          576 t
ORIGIN      
        1 gcgggactca gggctgacgg tggggcagcg ttttccgttg aactcgagag cgaagagcgt
       61 ctcgtagtgg tccatgagat ccagacgcgt tgtacgacgg aagccagtga gattatcgcg
      121 gcgatccggg ccgtaatcgc ggaggagttt gagatacaac ctgcggcggt ggtgttgatc
      181 aggccgggta ctcttccgaa aacttccagc ggcaaggttc gtcgtggcgc ttgtcgcgag
      241 cgctttctgg aaaatcgctt gcgcgtgatc gcggagtggc gcgcgaccgg cgagcgaaac
      301 ggttttacgc ctgcggcgcc tgaagagttg aatgcggaga ccatcgagcg atggctacgc
      361 ctgttgttga gtgcaaggct gagtcttgat ggtccactta tcgatgcccg acagccgatc
      421 gctcgctatg gcatcgactc tttgctggct ctcgaattag cccatgcggt cgaaacgact
      481 ctcgggataa gactgtcgcc ggcgagcctg ctgcggaacc ccattgtcga actcacaaac
      541 gagatactgg tccagcttgc gcgcaccgaa cactcgcatg cggtagcaca tgtggcgacg
      601 gtggatcaga gcggcgagca ttctttgtcg catggacaac aagcgctctg gacgttgcaa
      661 caagcgtcgc cggagagcac cgcctacaac gtctcggccg ctgcgcgcgt ccgcggcgag
      721 gttgatacgc gtgcgctgca acgcgcgttc agaatcttga gccggcgcca ttcgatgttg
      781 cgcgcgcgct ttccttcacg acagggcgcg ccggtgtgcg ccatacagga acactcagcc
      841 atctcgtttc gcatcgagga tgcgatgagt tggtccgaag agaagttaaa caatcgtctc
      901 cgggacgaag catggaccag tttcgatctc gaacgcgggc cgttattgcg cgccggtctg
      961 ttcaagagat ccgagcggga gtatgtgatg ttgctcagcg cgcaccacat tatcgttgac
     1021 ttctggtcgc tcgcagtttt gatacgcgaa ctcggccagt tgtatgaagc ggaggtcgaa
     1081 gacaggcctt tggacctcgc agcggtccga gcctactccg actacgttcg ccgccagaca
     1141 gaattgctgg ccagttccga gggtgagcgt tcgagaaagt actggcttca gcagttagcg
     1201 ggcgaactgc ctgtgctcga tctgccggcg gatcgcgcgc gtcctcccat tcagagttat
     1261 cgtggcgcgt cagtatcagc ccgcctggac cagcaattga cggagcagtt gaagaagctc
     1321 gctatgagtc atgaagccac gttattcatg actctgctcg ccgcatttca ggtgctgctg
     1381 taccggtata cgggccagga cgagatcctc ataggctcgc catcgtcagg ccgtgggtgg
     1441 gcggaatttg ctggaacagt tggctacttc gtcaatccat tggtgctgcg agcccagatt
     1501 tcgagcgaaa gatctttctc ggactttctc actgagacgc ggcagaccgc actcggggct
     1561 tttgagcacc aggattatcc gttcaatcta gtcgtcaagc agttgcagcc ggaacgcgat
     1621 cccgcgcgct cacctctgtt ccaggtaatg tttgcgttgc aaaagggcga ggccgctttg
     1681 aaactcggcg gcttgccggt cgagccgatc gcgttggacc atcgtgccgc acagttcgag
     1741 ctatcgttaa cggtagttga ggttgactcc ggactcacgg cgtcgttcga gtacaacacg
     1801 gatctgtttg acgccttgac catcgaacgc ctgagcggac atttccgcat cctgctcgag
     1861 agtgttgtta ccgatccgtc agtcagcttg tcagagctgc cgctactctc agatgccacg
     1921 cggcaacaga ttttgtacga ctggaaccac acgcagacgc gggtttcacc cgttgtttgc
     1981 gtgcatgaat cgttcgcgca acaggcggcc tcttcgcccg atcggatcgc gatcgattcc
     2041 catgaagaaa gcctcagcta tcgcgagttg aataaacgcg cgaatcggat ggcgcattac
     2101 ctgcgtgggc ggggcgtggg accggaagtg ctcgtgggta tatgcgttcc gcgttccgct
     2161 gcgatgttga cgtgcgtgct cggcgtgctc aaggcgggtg gcgcttacgt tccgctcgat
     2221 ccttcgtatc cggaagagcg tttggcgttc atgctcgagg atagtggcgt ttcgttgctg
     2281 cttaccgagc agctcctcga ggttgacggt ccagcaattg atgccgagag tgatgccgat
     2341 ccgatacctt ccgcaacctt cgaccatctc gcgtatgtga tctacacatc cggctcgact
     2401 ggaatgccga agggcgtgat ggtccaacac ggctctttgg caaattacgt ccacgcggca
     2461 aacgtcgttt acggcgtcgg gccggaggac cgggttcttc agttttcatc tttcagcttc
     2521 gatgcccatg cggaagagat ttttgtatct ctcacaagc
//
LOCUS       DASTEY010000063         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1501048, whole genome shotgun sequence.
ACCESSION   DASTEY010000063 DASTEY010000000
VERSION     DASTEY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501048"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFO72_01095"
     CDS             <1..578
                     /locus_tag="VFO72_01095"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HET9477907.1"
                     /translation="QTLRMMDTLMPKLLRVSLFLVMLAFVSGFAECYKPVTKNQLPSR
                     IKTVAVPAFQMESNALRYKIQSRFTEAVMKELVHRGRGLRVQGEREGADAVIEGVIKS
                     FNFAGVLLDDKGRARIFEVTIAAAVTVRDQTENKVLYDNQNFVFRGEYEFASDPRSFF
                     NEEDPAILRMSRSFAESIVSTLINAVEIGKK"
     gene            575..1570
                     /gene="holA"
                     /locus_tag="VFO72_01100"
     CDS             575..1570
                     /gene="holA"
                     /locus_tag="VFO72_01100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET9477908.1"
                     /translation="MKALSRAELERSLRDQPQPLYLLLGTERFLRDAAARSITEAALR
                     GTLLREFNESSFNLLRDSVQAAIAAAEQLPMMSDWRVVRIRDFGRLRESDEETLVRYL
                     DNPSPSTVMIFIADDLDKRKKATRALLDNCVVDDFPSLKDAEAKARAKARLKELKVTA
                     DDSVLSEIINLVGTDVQTLFNELDKLASAVADTGRITPAAVDELIGRSRELSNFELGD
                     HLLANNRKRALETLHRLLENGAEPVMLIGLIAGNYHRLALAKHLLAKGGREEVFRNIS
                     LPPFKRDAYIANLKRSDAAKIARGLQLTAAADLAIKTSQATPRLQLEMLVCELAS"
     gene            complement(2003..2269)
                     /gene="rpsT"
                     /locus_tag="VFO72_01105"
     CDS             complement(2003..2269)
                     /gene="rpsT"
                     /locus_tag="VFO72_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET9477909.1"
                     /translation="MPNHKSSEKRVRQSEKRRVINRGHRTKVRTYIKKVRTALASGSK
                     QEVQRVLPEAISVIDKSVQKGVMHANAAARYKSRLTSQANQAGK"
     gene            2392..3345
                     /locus_tag="VFO72_01110"
     CDS             2392..3345
                     /locus_tag="VFO72_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9477910.1"
                     /translation="MIKIELPPRGLETLFGVHDQNIKYLESLLDVRIDARGQDVSIDG
                     DPTDVQTAQRILEDFSDLFKEGKHFTDKELREAFAQIAEDRAFSLRDYFTKARFNPAG
                     KKQVAPKSANQRKYIQAIQEKDVVFGIGVAGTGKTYLAVAMAVQALMQKQVNRIVLAR
                     PAVEAGEKLGFLPGDLQDKVDPYLRPLYDALFDLIDYERVTRLLEKRVIEVAPLAFMR
                     GRTLSDAFIILDEAQNTTSEQMKMFLTRIGFGSKAVITGDVTQVDLPTGKRSGLIEAE
                     RILSNLEGIEFVYFTDKDVVRHQLVQMIIRAYEEQGKKSQM"
     gene            3351..>3740
                     /gene="ybeY"
                     /locus_tag="VFO72_01115"
     CDS             3351..>3740
                     /gene="ybeY"
                     /locus_tag="VFO72_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9477911.1"
                     /translation="MIEAVNRQRKTKIDAKEWESFADKAANAIGKSGSSATIAFVSDK
                     MIRQLNRQFRGVDKATDVLSFPAGDADETNLGDIAISVETAARQARENSLTFDQEVAQ
                     LILHGLLHLSGYDHETDNGEMNRLELRL"
BASE COUNT          878 a         1060 c         1012 g          790 t
ORIGIN      
        1 ctcaaacgtt gcgtatgatg gacaccctta tgcccaagct gctgcgtgtt tccctgtttc
       61 tcgtgatgct cgccttcgtt tccggttttg cggagtgcta taagccggtg acaaaaaacc
      121 aattgccgtc gcggatcaag actgttgcgg tgccggcgtt tcagatggag agcaacgcgc
      181 tacgttacaa gatccaatca cgcttcactg aagctgtaat gaaagagttg gtccatcgcg
      241 gccgcgggct ccgtgtgcag ggagaacgtg aaggcgccga cgccgtgatc gaaggtgtca
      301 tcaagagttt caactttgcc ggtgtgctgc tcgacgacaa gggacgcgcg cgcatttttg
      361 aagtcacaat tgcggccgct gtcacggtgc gtgaccaaac ggaaaacaag gtgctctacg
      421 ataatcagaa cttcgtcttt cgcggcgagt acgagtttgc cagcgaccca cgcagttttt
      481 tcaacgaaga agatccagcc attctcagaa tgtcgcgcag tttcgccgaa agcatcgtgt
      541 ccacgctcat caacgccgtt gagatcggaa agaaatgaag gccctgtccc gagcagagct
      601 cgaacggtct ttgcgcgacc aaccgcagcc gctctacctg cttctcggta cggagagatt
      661 tctgcgcgac gcggcggcgc gttcaattac cgaagcagcg cttcgcggca cgctgctgcg
      721 cgagttcaat gagtcgagtt tcaacctgct gcgcgattcg gtccaggcgg cgatagcggc
      781 cgccgaacag ttgccgatga tgtctgactg gcgggtggtc agaattcgcg acttcggcag
      841 gctgcgtgaa agcgacgaag agacgctggt tcgctatctc gacaatcctt cgccgtcgac
      901 ggtgatgatc ttcatcgctg acgatctcga caaacgaaaa aaagccacca gggcgctgct
      961 cgacaattgc gtcgtggacg actttccgtc gctcaaagac gcggaagcaa aagcgagggc
     1021 caaagcgcgt ctgaaggaac tgaaagttac tgccgacgat tcggtgctga gtgaaatcat
     1081 caacttggtc ggcacggacg tgcagacgct ctttaatgaa ctcgataaac tcgcatctgc
     1141 tgtcgccgat actggtcgca tcacgccggc tgctgttgat gaactgatcg gtcgctcgcg
     1201 cgagttgtcg aacttcgaac tcggagacca cctcctcgcg aacaatcgga agcgtgcact
     1261 cgagactctg catcgactgc tcgaaaacgg tgcggaaccg gtgatgctca tcggcctcat
     1321 tgccggaaac tatcatcggc tcgcgttggc gaaacatctg ctcgcgaaag gcggacgcga
     1381 agaggtcttt cgtaacatct cactacctcc ttttaagcgc gacgcataca tcgccaacct
     1441 gaaacgcagc gacgccgcga agatcgcgcg cggcctgcaa ctcaccgccg cggcagacct
     1501 cgctatcaag acttcacaag cgactcctcg acttcaactg gagatgctgg tgtgcgaact
     1561 ggcgagttag agcctgatat gtcgcctgtc gagctaacga aggcgccgat caccaggttg
     1621 catttctcga cgagcgacgc ttgcgtccca atctccgcta actgtgtcgc gactgcgttt
     1681 atccaaaaaa cagtgatctc ctcgctgtac ttcttcctgt ctacaccgtg atgatccaag
     1741 aacctcaata gcgcagttct cattttcccc acggtctcag cggccgaaag cgtttgcaaa
     1801 taccacacgg caaccaacaa atgctcgcga tggtgaaagt catccgccgg cgtctcgcac
     1861 gtttcaaatc cacgaacaac ttgttcgatc tcttcttctg actcgtaaag acggctcatc
     1921 ggtttctcct ttatagcaaa caaaaagcga ggcgggccga aatatcgacc cgcctcgcgg
     1981 ttactcaaaa cctggttagc ttttacttgc cggcttggtt cgcctgtgac gttagtctag
     2041 atttataacg agctgccgca ttcgcatgca tcacgccctt ttggactgac ttgtcgatca
     2101 cggaaatcgc ttcaggtaga acgcgctgga cttcctgctt cgatccggac gccagtgccg
     2161 tgcgcacttt tttgatatag gtcctgacct tggtgcgatg gccacgattg ataacgcggc
     2221 gcttttcgct ctggcgcacg cgcttctcgg atgatttgtg attcggcatt aactccctct
     2281 cctaagcgcc tcaggcgcaa gtggaggagt atatggacgc ctcaccggag tgtcaaggcg
     2341 cgccagcccg tccacaccga caacacagat ccgtgctaat ctgtgtggcc aatgataaag
     2401 atcgagcttc cgccgagggg tttggagacg ctgtttggtg ttcacgacca gaacatcaag
     2461 tatctcgagt ctctactcga cgtgagaatc gacgcgcgcg gacaggacgt gtccatcgat
     2521 ggcgacccga ccgacgtcca aacagcgcaa cgcatcctcg aagacttttc cgacctattc
     2581 aaagaaggaa aacacttcac cgataaagaa ctgcgcgagg ccttcgcaca gatcgccgaa
     2641 gaccgcgcct tcagtctccg cgattacttc accaaagcac ggttcaatcc agccggcaag
     2701 aagcaggtgg cgccgaagtc tgcgaaccag cgcaagtaca ttcaggcgat tcaggaaaaa
     2761 gacgttgtgt tcggcatcgg cgtcgcggga accggcaaga cttatctggc cgtggcgatg
     2821 gccgtccagg cgttgatgca aaaacaggtg aaccgaatcg tgctggcgcg ccccgcggtc
     2881 gaagcaggtg aaaaactcgg ctttcttccc ggcgacttac aagacaaagt cgatccgtat
     2941 ctgcgtccgc tttacgatgc gctgttcgat cttatcgact acgaacgcgt aacgcgcttg
     3001 ctggaaaaga gagtcatcga agttgcgccg ctggctttca tgcgtggccg cacgctctca
     3061 gatgcgttca tcatcctcga tgaagcgcaa aacaccacca gcgaacagat gaaaatgttt
     3121 ctcacgcgca tcggcttcgg ctccaaagcc gtgatcaccg gcgacgtgac ccaggtcgac
     3181 ctgccgacag gaaaacgcag tggtctaatc gaagcagaac gaatcctgtc gaaccttgaa
     3241 ggcatcgagt ttgtttactt cactgataaa gacgtggtgc gtcaccaact ggtgcagatg
     3301 atcatccggg cttacgaaga acagggtaag aagagccaga tgtagtcacg atgatcgaag
     3361 cagtcaaccg gcagcggaaa acaaagatcg acgctaaaga atgggagtct ttcgcggaca
     3421 aggcggccaa cgccatcggc aagagtggtt cgtcggcaac aatcgctttc gtttccgaca
     3481 agatgattcg acaactcaac cggcaatttc gcggcgtcga caaagccacg gacgtgctct
     3541 ccttcccggc cggcgatgcg gacgaaacaa atcttggcga catcgcaatt tcagttgaga
     3601 ccgccgcgag acaggcacgc gaaaatagtc tgacgtttga ccaggaagtg gcccagttga
     3661 tcctgcacgg gctgctgcac ctgtctggtt acgatcacga gacggacaac ggcgagatga
     3721 atcgtttgga actccggctc
//
LOCUS       DASTEY010000064         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_940807, whole genome shotgun sequence.
ACCESSION   DASTEY010000064 DASTEY010000000
VERSION     DASTEY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940807"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFO72_01120"
     CDS             <1..1118
                     /locus_tag="VFO72_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477912.1"
                     /translation="FTRPMLDLHIRRRLAQNPKIEICDQMDVLGLLRDENENRVAGVL
                     VSPRHSEADRRVAKPVYAELVVDATGRASRAPQWLKDLGYEPPKETVISAHLGYASRL
                     YRIPEGFNGDWTCAYVQSAPPQRKRGGILFRVEEDRWLVTLIGGGRDYPPSDEQGFLQ
                     FADTLPVSIIADAIRGAEPLAPVRTHRATENRLRHFESARVLPENFIAIGDAACAFNP
                     VYGQGMTIAALGAVTLRDCLDEQRRLRSDGSLAGFARRFQKRLARVNKTPWLMATGED
                     YRYQETVGGSPSLTTRFMHWYLDRVLVLATREVAVRSLVLRVFSMVLPPSALFRRAIL
                     LRVIREAIRPAPPRDATPRTRKPVLYKVEARYGRETS"
     gene            complement(1305..>2122)
                     /locus_tag="VFO72_01125"
     CDS             complement(1305..>2122)
                     /locus_tag="VFO72_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9477913.1"
                     /translation="LKDARVVAATLTGSEPAGSSVASIAGKAIKKTVLELGGSDPFIV
                     MPSANLAEAVTTAVKARTINNGQSCIAAKRFIVAAEVYPQFERGFVDQMNALRVGDPF
                     EEETEIGPLATAQIVADLERQVKQAVAEGARILTGGKRLDRPGNFYEPTVLVDVNPSA
                     PVSCEEIFGPVAMLFRVNDIDEAIRLANATSFGLGSAAWTNDAGEQRRFIEELEAGSV
                     FINGMVASDPRLPFGGVKHSGYGRELAEFGIREFVNIKTVWIGENKAATDEIE"
BASE COUNT          419 a          624 c          626 g          453 t
ORIGIN      
        1 cgttcacgcg tccgatgctc gacctgcaca tccgccgcag gctcgcacaa aatccgaaga
       61 tcgagatttg cgaccaaatg gacgtgctgg gccttttgcg cgacgagaac gagaaccgcg
      121 tcgccggcgt cctcgtcagt cctcgtcatt ctgaggcgga cagacgtgtc gcgaaaccgg
      181 tttatgctga gctggttgtt gatgcgactg gccgcgcgtc gcgcgcaccg cagtggctga
      241 aagatttggg atacgaaccg ccgaaggaga cggtgatcag tgcgcacctc ggttatgcca
      301 gccggttgta tcgcattcca gagggcttta acggcgattg gacgtgtgcc tacgtgcagt
      361 ccgctccacc gcagcgaaag cgtggcggga ttctctttcg cgtcgaagag gaccgctggc
      421 ttgtgactct tatcggtggc ggacgcgact atccgccaag tgacgaacag ggctttcttc
      481 agtttgcaga taccttgccg gtctcgatca ttgccgacgc aatccggggc gccgagccgc
      541 tggctccggt tcgcacacac cgtgcgacag aaaaccgcct gcgccacttt gaaagcgccc
      601 gggttctgcc ggagaacttc attgccatcg gcgatgcagc gtgcgccttc aacccggtct
      661 atggacaggg tatgacgata gcggcactgg gcgccgtgac gcttcgagac tgtttagacg
      721 agcaacgaag attgcgttcc gatggaagtc tcgcgggctt tgcgcggcgc tttcagaaac
      781 gcctggcccg agtcaacaag acgccatggc tgatggccac gggcgaagat tatcgctatc
      841 aggagaccgt aggtggttca ccaagtctga cgacgcgctt catgcactgg tacctcgatc
      901 gcgtgctcgt tttggccacg cgcgaagttg cggtgcgcag tcttgtgttg cgggtgttca
      961 gcatggtgtt gccgccgtcg gcgctgtttc gacgcgcgat cctgctgcgc gtaatccgcg
     1021 aagcgattag acccgctccg ccacgcgacg ccacaccgcg aacacgaaaa cccgtgttgt
     1081 acaaagttga ggcccgttac ggaagggaaa cgtcatgaag acacgtaccg aaataacggt
     1141 tgaaatggat cgggtgatcg ttgtgagtcg ctcgcgaaag tcggagtggt gcgcttgcgg
     1201 ctcgcgcctg gccgagggat tcagtatcga ggatgcggag ctcgttgccc aaaggctcgg
     1261 cgatcggccc gaactcgccg aaaatctttc ggcgttaatc agtgctattc gatctcatcc
     1321 gtggccgctt tgttttcacc aatccaaacc gtcttgatgt tcacgaactc gcggatgcca
     1381 aactccgcga gttcgcggcc atagccggaa tgtttgacgc caccgaacgg caggcgcgga
     1441 tcggaagcaa ccatgccgtt gatgaaaacc gatcctgctt cgagttcctc gatgaagcgg
     1501 cgttgctcgc ctgcgtcgtt ggtccacgca gctgaaccga ggccgaaaga cgtcgcgttc
     1561 gcgagacgaa tcgcttcgtc aatgtcgttc acacggaaca gcattgctac gggaccaaag
     1621 atctcctcgc acgaaacagg cgctgatgga ttgacgtcga cgagtacggt tggttcgtag
     1681 aaattgcctg gacgatcgag tcgcttgccg cctgttagaa tccgcgcgcc ttccgctaca
     1741 gcttgcttca cttgtcgttc aagatcggcg acgatttgcg ccgtcgccag cgggccaatc
     1801 tccgtttctt cttcgaaagg gtctccaacg cgcagtgcat tcatctggtc gacaaatccc
     1861 cgctcaaact gcggataaac ttccgctgca acgataaagc gcttcgccgc gatgcacgac
     1921 tggccgttat tgatcgtgcg tgctttcaca gcagtcgtaa ctgcctcggc gaggttcgct
     1981 gacggcatga cgataaacgg atcgctgccg ccgagttcca gaacagtctt cttgatcgct
     2041 ttgccggcga tacttgcgac gctgcttccc gccggttcac tcccggtcaa cgtcgccgca
     2101 actacccgcg cgtctttcaa aa
//
LOCUS       DASTEY010000065         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_990186, whole genome shotgun sequence.
ACCESSION   DASTEY010000065 DASTEY010000000
VERSION     DASTEY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990186"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1295)
                     /locus_tag="VFO72_01130"
     CDS             complement(255..1295)
                     /locus_tag="VFO72_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9477914.1"
                     /translation="MGFDPRNILVIDFGQLGDVVMSLPALRAIRERFPNAQITVAVGK
                     PGKELLSLSGYANEILEVDRVGLRDGPTLVSIGRILKLVSQVRKAKFDFVIDLHSYYE
                     TNLLGFFSGARHRLYSRRETNRSLYFLANFKPQPVKESEKTHLVDRYLDVVKPLGIQN
                     APRTPVLKTSAADATSVDALLKKEHAQSGELLAGIFPGAGNRSRIWPLEHFAEVADHL
                     RRNDKVRIIVFAGPEERQMVPRMRKLFPAGTIFFDRLTIPQLAAAQARLTVFVSNDTG
                     PVHIAAAVGTPVVVIMDRLDPHCFTPVGAEHRLVYGSSVDSISVEKVYKAAHELLALS
                     RTDTLFKVDKAT"
     gene            complement(1359..>2428)
                     /gene="gpmI"
                     /locus_tag="VFO72_01135"
     CDS             complement(1359..>2428)
                     /gene="gpmI"
                     /locus_tag="VFO72_01135"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9477915.1"
                     /translation="SGATYVATLQRKLLEIGCGEIATVVGRYYAMDRDKRWERTQRAY
                     DLLTKAIGSHANDPIAAIRESYERGVTDEFVEPIAIVRKDLSPVATIKDDDSVIFFNF
                     RPDRARQLTRALTIQDFKEFPVLDRPHVDYVCFTMYDTTFNLPVAFPPRQHHNVLAEV
                     WSNTSVRNYRMAETEKYAHVTYFFNGGVEREYPCEQRLLVPSPKIATYDLQPEMSAFK
                     VTDKVLRGIDEGDTDVFVINFANPDMVGHTGKLDKTIEACQYVDSCLGWITKRVREAR
                     GITLITADHGNAEQMIDPVTGSPHTAHTTNPVPFHLIDEESIGVQLRSGGALEDVAPT
                     LLGLLGVEKPAEMTGRDLRYL"
BASE COUNT          510 a          653 c          716 g          549 t
ORIGIN      
        1 atcaggcaaa gcgtgattaa gcataatctc ttcagcattc taagcattgt tgtctccata
       61 attcgctgag gcaggtgaac ttggaaagaa tcgtgaggcg ggcataccaa acgagcccaa
      121 gccatactaa cgaagttaaa ccatgtttgt ctacagggat tttgattgtg ggcgtaattg
      181 taggggcggc cctctgtggc cgccccagat gacgtgcgcc ggggcggcca cggagggccg
      241 cccctacaca agttttacgt ggccttatcg actttgaaga gagtgtcggt gcggctgagg
      301 gcgaggagtt cgtgtgcggc tttatagact ttctcgaccg aaatgctgtc cacagaggag
      361 ccgtagacga gccggtgctc tgcgccaaca ggggtaaaac aatgcggatc caggcggtcc
      421 atgatgacca ctacgggcgt ccctaccgcc gccgcaatgt gcaccgggcc ggtgtcgttg
      481 gagacaaata cggtgagacg ggcttgggcg gccgcaagtt gggggatggt cagccggtca
      541 aagaagatcg tcccggccgg aaaaagcttt cgcatccggg gcaccatttg tctctcttcc
      601 ggtccggcga agacgatgat ccggactttg tcgtttcgtc gcaggtgatc ggcgacctcg
      661 gcaaagtgtt ccaggggcca gatgcgggag cggttgccgg cgcccggaaa gatgcccgcg
      721 agtaactcac ccgactgggc gtgctccttt ttcagcagcg cgtcgaccga cgtagcgtcc
      781 gcggcacttg tcttcaatac cggagttcgc ggcgcattct gaatcccgag cggctttact
      841 acgtccaggt agcgatcaac gagatgagtt ttctccgatt ctttgacggg ttgcggtttg
      901 aagttggcga ggaagtagag cgaacgatta gtttcgcggc gcgagtagag tcgatgacgc
      961 gctccggaga agaacccgag caagtttgtc tcgtagtaac tgtgcaggtc gattacgaaa
     1021 tcaaacttcg ccttgcggac ctgtgaaacc aacttcagaa tgcggccaat tgaaacgagg
     1081 gttgggccat cgcgcagtcc tacgcgatcc acttcaagga tttcgttcgc ataaccggag
     1141 agactcagca gctctttgcc aggcttgccg accgcaacgg tgatctgcgc attcggaaat
     1201 cgctcgcgaa tagcgcgcag cgccggcaaa ctcattacga catcgccgag ttgaccaaaa
     1261 tcgatgacga gtatgttgcg cggatcaaat cccattttta taagccgcag attttcgcaa
     1321 ataaacgcag atctgcgttt gtccgcgaaa atctccggct aaagatatct caaatcgcga
     1381 cccgtcatct cagctggttt ttcgactccc aacagaccga gcagcgttgg cgccacgtct
     1441 tcgagcgcgc cgccgctacg gagttgcacg ccgatcgatt cttcgtcaat caagtgaaaa
     1501 ggaaccgggt tggtcgtgtg tgccgtatgc gggctgccgg ttacgggatc gatcatctgc
     1561 tcggcgttgc catgatcggc ggtgatgagc gtgatgccgc gtgcttcgcg cacacgcttt
     1621 gtgatccatc cgaggcagct atcgacgtac tggcaggctt cgatcgtctt gtccagtttg
     1681 cccgtgtggc ccaccatgtc aggattcgcg aaattgatga cgaaaacgtc cgtgtcgccc
     1741 tcatcgatgc cgcgcagcac tttgtccgtc actttgaaag ccgacatctc gggttgcaga
     1801 tcgtaagtcg cgatctttgg cgacggcacc agcagacgtt gttcgcacgg atactccctt
     1861 tcgacgccgc cgttgaagaa gtatgtgacg tgcgcgtact tctcggtttc agccatccgg
     1921 tagttgcgca cgctggtgtt gctccaaact tccgccagca cattgtgatg ttgtcgcggt
     1981 ggaaacgcta ctggaaggtt gaacgtggtg tcgtacatcg tgaagcagac atagtcgacg
     2041 tggggccgat cgagaacggg aaactctttg aaatcctgga tcgtcagggc tctcgttaac
     2101 tgccgcgcgc gatcaggacg gaagttaaag aagattaccg agtcgtcgtc tttgatcgta
     2161 gccaccgggc tgaggtcttt acgcacgatc gcgattggct ccacgaattc atcggtaacg
     2221 ccacgctcgt acgactctct aatcgcggca atcgggtcgt tcgcgtgtga accgatcgct
     2281 ttcgttaaca gatcgtaagc gcgttgtgtt ctttcccagc gtttgtcgcg atccatcgcg
     2341 tagtaacggc cgacgaccgt ggcgatctcg ccgcagccga tttcgagcag cttgcgctga
     2401 agagtcgcga cgtaagtcgc gccagatg
//
LOCUS       DASTEY010000066         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1517726, whole genome shotgun sequence.
ACCESSION   DASTEY010000066 DASTEY010000000
VERSION     DASTEY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517726"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VFO72_01140"
     CDS             <1..961
                     /locus_tag="VFO72_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9477916.1"
                     /translation="TVESTLRSGTSLVELMSALFPCGSVTGAPKIRTMQIIHELESFP
                     RGAYTGAIGFLRPGGDCVFNVAIRTVVIDTDTGVATFGVGGGVTIDSTAAGEYEECLV
                     KSRFLQSKPVEFELFETMLIENGEIFLLERHLERLRSSAAFFGFNLKTEALYELTARI
                     ASQPAKLKLTLRKDGRLRIETSPITNPTDLKRVTIATEPVDSSDPFLFHKTTHRDFYS
                     EQLKLRPDCDDIIFCNERGEVTESTIANVVVRLGDELFTPPVSCGLLAGTFRDQLLEE
                     GKITERVITLDELDNAAELYLINSVRKWMKAVCVYPSSSVAQL"
     gene            complement(952..3387)
                     /locus_tag="VFO72_01145"
     CDS             complement(952..3387)
                     /locus_tag="VFO72_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HET9477917.1"
                     /translation="MTTRILLILLLVAQPALAQLQLAKPETVSVSSVALERMDSVIQD
                     EIGKRRLPGAVVLVGRKGRVIWRKSYGSRVVEPAREVMTADTIFDVASLTKVVATATS
                     IMILVERGKVRLNDPVSVYIPELKGEGRENITIEKLLIHRSGYAPDFDLSERWTGYDE
                     AIKRLTIEPLRNPPGTRFVYSDIGFIALGEVVKRVSGMPLDQFAKQNIFQPLRMSDTG
                     FRPGAALRSRIAPTEKRRGQLSYLGDTPAGIGAEGDVWLRGQVHDPTSDRMNGVAGHA
                     GLFSTAKDLAVYCQMILNGGEYGGIRVLSPLSVAEMTRPRLISNTGATRGLGWDINSS
                     FSTNRGELFPLGSFGHTGFTGTSIWIDPSSQMFVVFLSNRVHPDGKGDVGPLRGRVAS
                     IVAGAITDASVSQHAREQSANYYAEIVKALQYFAPRHEGTATSVKVLTGIDVLERDNF
                     KPLAGLRIGLVTNHTGRNREGRQTIDVLNKASGVKLVALFSPEHGIRGLADENVSDSK
                     DEATGLPIYSLYGDTRRPKPEQLKDLDALVYDIQDIGARFYTYISTLGYILEEAAKAK
                     LPVFVLDRPNPIGGVDVEGPTADKDKLSFISYHTIPTRHGLTVGELAQLFNRQRNIGA
                     DLRVIKMEGWRRSMWFDETNLTWINPSPNMRSLTEATLYPGVGLLETTNVSVGRGTDT
                     PFEIVGAPWIQGDKLADHLNRRGIPGVRFVPVRFTPEASVFKDEQCGGVNIIITDRVA
                     FQPLLTGIEMALALRQLYPAEWKVDNYLRLLVNADTLERIKRGESARDIVSSWNASLQ
                     EFRRARAEILLYN"
     gene            complement(3384..>4019)
                     /locus_tag="VFO72_01150"
     CDS             complement(3384..>4019)
                     /locus_tag="VFO72_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9477918.1"
                     /translation="VEQRLTPIDTIDRVVGSKDVVKLATDIAEHAVTLVRDDDKLVPL
                     KDLKPESKVFNLAITNGEDRNWIANAFVSILNRSGVKVETLVLDERSSEQEVQKAVER
                     AKAADLVFASLYGRVRSGQASSVGLPDSGVRALSAVIAAKKPIVGISFGNPYLLQSFP
                     GLRTYMVAYGDMPSLQHAVARALLGEIDIVGRLPISLPGLYPRGTGIQVKR"
BASE COUNT          889 a         1176 c         1084 g          870 t
ORIGIN      
        1 aacggtcgaa tcgacgttgc gatcggggac gagtttagtg gaactgatga gcgcgctgtt
       61 tccgtgtggt tcagtcacgg gcgcgccaaa gattcggacg atgcagatca ttcacgagct
      121 cgaaagtttc ccgcgtggcg cttacacggg cgcgatcggg tttttgcgtc ccggcggaga
      181 ctgcgttttc aacgtcgcca ttcgcacggt cgtgatcgac acggacacgg gcgtggcgac
      241 gtttggcgtc ggcggtggag taacgatcga ttcgacggcg gccggtgagt atgaagaatg
      301 cctggtgaag agccggtttt tgcagagcaa gccggtggag tttgagttgt ttgagacgat
      361 gctaatcgaa aacggcgaga tctttctgct cgaacgacac ctcgagagac taagaagttc
      421 agcagctttt tttggtttta atcttaaaac agaggcgctc tacgaactca cggcccgtat
      481 tgcatcccaa ccggcgaagt taaagctgac actgcgaaag gatgggcgcc ttagaatcga
      541 aacctcccca atcacgaacc caaccgatct gaagcgcgtc accatcgcga ccgaacccgt
      601 cgacagctcc gatccctttc tctttcacaa aacaacccat cgcgacttct actcagaaca
      661 actaaaactg cgccccgact gcgacgacat catcttctgc aacgagcgcg gcgaagtaac
      721 agagtcgacc atcgcgaacg tagtcgtgcg tctcggcgac gaactgttca cgccgcccgt
      781 tagctgtgga ctgctggcgg gcacgtttcg cgatcaattg cttgaggaag gaaagatcac
      841 agaacgagtg atcacgctcg acgaactcga caacgccgcg gaactctatc taatcaactc
      901 agtgcgcaaa tggatgaaag ctgtctgcgt ttatccgagt tcgtccgtgg ctcagttgta
      961 gagcaggatc tccgcacgcg cacgccgaaa ctcctgtaac gacgcattcc acgacgacac
     1021 aatatctcgc gcactctcgc cacgcttgat ccgttccaac gtgtcggcat tcaccagcaa
     1081 ccgcaggtag ttatcgacct tccactcggc cgggtacaac tgccgtaacg ccaatgccat
     1141 ttcgataccc gtcaacaagg gttgaaacgc cacgcgatcc gtgatgatga tattcacgcc
     1201 gccacactgc tcatctttga agaccgacgc ctctggcgtg aaacgaacag gcacaaaccg
     1261 tacacccgga atgcctcgtc gattcagatg gtcagccaat ttatcgccct gaatccaagg
     1321 cgcgccgaca atctcaaacg gcgtgtcggt gcctcgacca accgagacat tcgtcgtctc
     1381 caacaagccg acgcccggat agagcgtcgc ttccgtaaga ctgcgcatgt ttggcgacgg
     1441 attgatccac gtcaggttag tttcatcaaa ccacatcgag cggcgccagc cttccatctt
     1501 gatgacgcgc agatccgcgc caatgtttcg ctgtcgattg aagagttggg ccagttcgcc
     1561 aaccgtcagg ccgtgtcgcg tcggaatcgt gtgataggag atgaacgaga gtttgtcttt
     1621 gtctgcagtt ggaccttcga cgtctactcc accgatcggg ttcggccggt cgagaacgaa
     1681 cacaggaagt ttggctttcg ctgcttcttc gaggatgtag ccgagcgtgg atatgtatgt
     1741 atagaaacgt gcgccgatgt cctggatgtc gtagaccagc gcgtcgagat cttttagttg
     1801 ctccggtttt ggacgccgcg tatcgccata aagcgagtag atcggcaggc cggtcgcttc
     1861 gtctttggaa tcggaaacgt tttcatctgc cagtccgcga atgccatgtt cgggagagaa
     1921 cagcgctacc aatttcacac cggatgcttt attgagcacg tcgatggtct gccgtccttc
     1981 gcgatttcgc ccggtgtggt tcgtgaccaa accgattcgc aggcccgcga gtggtttgaa
     2041 gttatcgcgc tcgagcacat cgatgccggt caacaccttt accgaagtag ccgtgccctc
     2101 gtggcgcggt gcgaagtact gcaaggcttt gacaatttca gcgtagtaat tcgccgattg
     2161 ctcgcgggcg tgttgcgaca cgcttgcatc agtgatcgca cccgccacaa tcgaagcaac
     2221 gcgtccgcgc agtggtccaa catcgccttt gccgtccggg tgcacgcggt tactcagaaa
     2281 gacgacaaac atctggctcg agggatcgat ccagatcgac gtgcctgtga atccggtgtg
     2341 tccaaacgaa ccgagcggga agagttcgcc gcgattcgtt gaaaaactgc tgttgatgtc
     2401 ccaaccgaga ccgcgagtcg cgccagtgtt ggagatgaga cgcggccgcg tcatctcggc
     2461 cacagacaat ggggacaaaa cacggatacc gccatattcg ccaccgttta gaatcatctg
     2521 gcagtagacg gccaggtctt ttgcagtcga aaacagtccc gcgtgtcccg cgacgccgtt
     2581 cattcgatcc gacgttggat cgtgcacctg cccgcgtaac cagacatcgc cctcggcgcc
     2641 gatgccggcg ggcgtatccc cgagataact caactgtccg cgtcgttttt cagtgggcgc
     2701 gatccgcgag cgcaaagcgg cgccgggacg aaagccagta tcgctcatcc gcagtggctg
     2761 aaagatgttc tgcttcgcaa actgatcgag cggcattcca ctcacgcgct tcacgacctc
     2821 accgagggcg ataaagccga tgtcgctata gacgaaacgc gtgccgggag gattgcgcag
     2881 cggctctatg gttagccgtt tgattgcctc gtcgtaaccg gtccagcgct cgctgaggtc
     2941 gaagtcaggc gcgtagcccg agcgatgaat taaaagcttt tcgatagtga tgttttccct
     3001 accctcgcct ttgagctcgg gaatgtagac ggaaacagga tcgttgagac gtactttgcc
     3061 acgctcgacg agaatcatga tgctcgtcgc cgtggcaact actttcgtca aactcgcgac
     3121 gtcgaagatc gtgtccgcag tcattacttc gcgcgccggc tcaaccacac gcgatccata
     3181 acttttccgc cagataacac gacccttccg gcccaccaaa accaccgcac cgggcaagcg
     3241 gcgcttcccg atctcatcct ggattacaga gtccatccgc tccaacgcca cggacgacac
     3301 ggatacggtt tctggttttg cgagttgcag ttgcgccagc gccggctgcg caacgagcaa
     3361 gagtattaat aagatccgtg tcgtcatctt ttcacttgta tgccggttcc gcgtgggtaa
     3421 agtccgggca aagagatcgg cagcctgccc acaatatcga tttcgcccaa caatgcgcgc
     3481 gctacggcgt gttgcaaact cggcatgtcc ccataagcaa ccatgtacgt tcgcaaaccg
     3541 ggaaaacttt gcagcaagta cggattgccg aagctgatac ccacgatcgg cttcttcgct
     3601 gcaatcacgg cagaaagcgc ccgcacgcct gaatccggta agccgacgct cgaagcctgg
     3661 cccgatcgta cgcgtccata caacgaagca aataccagat cagccgcctt agctcgctcc
     3721 actgcctttt gcacttcctg ctcgctcgag cgctcatcca aaaccagggt ctcgactttc
     3781 acaccgctgc gattcagtat cgacacgaac gcgttcgcta tccagttccg atcctcaccg
     3841 ttggtaatcg cgaggttaaa gaccttcgat tcgggtttca ggtccttcag cggcacgagt
     3901 ttgtcgtcgt cacgaacaag tgtgactgcg tgttccgcta tatcggtagc cagttttacc
     3961 acgtccttac taccgaccac gcgatcgatg gtgtcgattg gtgtgagccg ttgttcgac
//
LOCUS       DASTEY010000067         8920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1385718, whole genome shotgun sequence.
ACCESSION   DASTEY010000067 DASTEY010000000
VERSION     DASTEY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385718"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFO72_01155"
     CDS             <1..935
                     /locus_tag="VFO72_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9477919.1"
                     /translation="RRDFTKLVTLSTLAAGFSTVPALARKPAPQFSITMDDFHWQNAV
                     KLTATERNQVILDTLRAHSIKAALFVIGRNIEEDEGKQLLSAWDKAGHVIANHTYSHR
                     NFNAPATDVNAYGEDILQAEALLKQFPRFQKYFRFPMLKEGDTAAKRDAMRAFLHQHG
                     YRNGHVTIDNSDWYIDQRLTARLKDNPAADVKPYRDYYLEHIWSRAMYYNALAHRVAG
                     YQVKHTILVHFNLLNALFLGDAIAMFKSKGWQPIDAEAAFTDPVFESKPNVIPAGESI
                     IWSLAKEKGTLAKSLRYPPEDSEYEAARMKKLGL"
     gene            932..1816
                     /locus_tag="VFO72_01160"
     CDS             932..1816
                     /locus_tag="VFO72_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9477920.1"
                     /translation="MNRLLLSTCILLVITSSVMGQVSDVAPTAAADPSSWKRYTAMDE
                     AFSIIFPTVPAMATSREYFENGQRERRIREIGAYADGIVYTVYSVENPGPKQSLERFI
                     KERFGNFKTREVTVSGVAGKEPVDQKGDLVRKYFATERHLYVFSTLGAPASDPRVQQF
                     FSSLVLGKNREGIEVSDGPGITLQPPVQAEPVDANIAPKSYVGREVDRKVRLGMKPQP
                     TYTNAARLNGLTGTVVLKVIFSSAGEVKNIRTVSGLPYGLTEKAIDAARKIKFIPAVK
                     EGKYVSVWMQLEYNFNLF"
     gene            1823..2848
                     /locus_tag="VFO72_01165"
     CDS             1823..2848
                     /locus_tag="VFO72_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9477921.1"
                     /translation="MPHLLEVKDLQTHFPTRAGLVRAVDGVSFYLDHGELLGLVGESG
                     CGKSITALSVMRLIAPPGRIVNGEIRFLGTNLLELSDAEMRRMRGDDIAMIFQDPMTS
                     LNPVFTVGEQIGEALRLHRQMSRKQAREATVEAMREVAIPDPARRINDYPHQLSGGMR
                     QRVMIAMALACNPKLLIADEPTTALDVTIQAQILELLNELRKQRDLAVLLITHDLGVV
                     AEVADRVAVMYTGRIVEESPVEELFARPKHPYTEGLLRSVPKLTSEHVAKRERLETIE
                     GVVPSPTDLPPGCHFAPRCPHRMPRCTEESIPLYQLEGGVQVRCVLFDLAAAVAADKQ
                     TTDFKSQ"
     gene            2848..3873
                     /locus_tag="VFO72_01170"
     CDS             2848..3873
                     /locus_tag="VFO72_01170"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET9477922.1"
                     /translation="MQQVQVQEQTRIFSPARDALVSVRGLVKHFPVEGSDDVVRAVDG
                     VSFDIFSGETLGLVGESGCGKSTVGRCLLRLIEPTAGQITFEGQDVLSLDKKRLRQLR
                     REMQIIFQDPYASLNPRMKVGDIIAEPLVIHNQGAKSERRERVASLLRRVGLDPNYMN
                     RYAHEFSGGQRQRIGVARTLALNPKLIVADEPVSALDVSVQAQVVNLLQELQEEFKLT
                     YLFISHGLAVVEHISTRVAVMYLGRIVEIAGAAELYARPLHPYTQALLSAIPIPDPRQ
                     KRKRIILQGDVPTPINPPSGCRFRTRCPLAIEECAHIDPELREVSPDHHVACIRVPGW
                     SEAPARL"
     gene            3939..4949
                     /locus_tag="VFO72_01175"
     CDS             3939..4949
                     /locus_tag="VFO72_01175"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET9477923.1"
                     /translation="MSAPQDPNFQAPPPPPTLEAEPEAPRATELRPFAIGVFVLGLIF
                     AGGGIARLLAGGIGTGASLCFFGLLLFALSFIRKPALPETERPLSWFEKVTGVFYEPT
                     RVFRNLRIHPYWIGAFLTIWVLTTIYSTAFIQRITPERITEYTVEKVSEMGPPFAPPP
                     EAIDRIRVEQLESLKNPVQRLGAVLKSMVSTFVFGAIAAALALLGILVFGGRINFWQA
                     LAVTFYATLPVVAVQKLLGLVILFLKTPDDLHPILNQETTLQDNLGVLISPADHPVIF
                     VIASFIGLTSFYGLWLRAKGLNQGATRVSKGQAWGVAITLWFLLLMVVVIFTALFPGF
                     IS"
     gene            complement(4961..6217)
                     /locus_tag="VFO72_01180"
     CDS             complement(4961..6217)
                     /locus_tag="VFO72_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477924.1"
                     /translation="MRILRSDIFENLMMALATLRANKLRSFLTVFGVVIGIITVMLIA
                     SIISGIDVSVTREVESFGTRSIYISKFNPGIHVGRRSREERMRKELTYDDAIALRSLP
                     TVELSVPFLDITNNWFGGRLMVSGGGKTSAQVALQGTLPEFEKAGTQVISEGRFFTQT
                     ENDTNQEVCVVGSKVADDFFKIGSPVGQTIKIGTQEFRVVGVLEKREQFLIGGGSDDQ
                     NNVIYVPFTVAQKLKPNADDIYILAVGKAGMMEEAKDQVRDLLRVRRQVPFAANDNFG
                     METSESILDNFRSITAGLAIAMVVISSVGLMVGGIGVMNIMLVSVTERTREIGVRKAI
                     GARRSDIMWQFLIEAATLTGLGGIVGLSIGWGLTLLLRMLLPSYVPLWAPIGGLIASV
                     GIGLIFGLWPAWKAARLDPIESLRYE"
     gene            complement(6219..7457)
                     /locus_tag="VFO72_01185"
     CDS             complement(6219..7457)
                     /locus_tag="VFO72_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477925.1"
                     /translation="MKLIETLKLAIAAIWAHKLRSALTLLGMIVGVTAFVVVVSLIEG
                     FNRYVDEKIAGIGAKSFTIQRFNPLEDFKDTDTIAAAQRRNKELTLGDYYYIKERAAL
                     IGQIGARARVTPSEVKRGEEVLEDIFVSGATANCVDIENRDVADGRFIAEPENEGAVR
                     VAYIGADIADKLFPGGNSVGQEISIRGLPYRVIGVEAAKGTVFGIPQDNFIIIPLKTY
                     SVNYGGLIRQRSLYFVATARSDELFNDAVEEARFLMRARRKLGPSEKDNFGIVTPDAI
                     TGLRDRLFGTIFIVAIAVPGIALVVGAIVIMNIMLVSVTERTKEIGIRKSLGARQIDI
                     LKQFLIEAVTLAVIGGAVGIFLAWIVGAIVTAWIFPTYLSILAIAGALAASGGAGVAS
                     GIFPAWKAARLDPIEALRAD"
     gene            complement(7578..8819)
                     /locus_tag="VFO72_01190"
     CDS             complement(7578..8819)
                     /locus_tag="VFO72_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9477926.1"
                     /translation="MRFEDIKESALMALDTLRTNKLRSSLTILGVSVGVVTVIFMVSI
                     IQGLNKAFADQIESLGSNTIFVSKFEPSFGRPPGPEEIHRKDLTMEDAEALRTEAPSI
                     AGVSPIQRLLSATIRYKDKQTDTPVLFGVTPYYEFVHSQYVGSGRFISEIDMQERSNV
                     VILGVDVKQALFPYEEAVDKEVRIEGTPYRIIGVMEPLGNFFGQSRDNSIFIPITTFA
                     KYYPDRPFPEVVFFVIVRPRSRAFVKNAMDEITDILRRRRRVPASAPNNFGISSQDSL
                     LDVYNQLTGATALVLTSISFVALMIGGIGVMNIMLVSVTERTKEIGVRKAVGATRLNI
                     LSQFLIEAVVLTAIGGLAGLAVGEVASLLMNKYSPLPAYVPLWAIGVGVGISAAVGIV
                     FGLWPAWKAARLNPIDALRWE"
BASE COUNT         1987 a         2566 c         2434 g         1933 t
ORIGIN      
        1 agcgaagaga cttcaccaaa cttgtaactc tcagcacgtt ggctgccggg ttcagcactg
       61 ttccggcgct ggcgcgcaaa cccgcgccgc agttttccat cacgatggac gacttccact
      121 ggcaaaacgc cgtcaaactc accgcgacgg agcgcaatca ggtgattttg gacacactgc
      181 gcgcgcattc gattaaagcg gcgctctttg tcatcggccg caacatcgaa gaggacgaag
      241 gtaaacagct tctttccgcg tgggacaagg ccggacatgt gatcgccaat cacacctact
      301 cgcatcgcaa ctttaatgcg cccgccacag acgtcaacgc ctacggcgaa gatattctgc
      361 aagcggaagc gttgctcaaa cagttcccgc ggttccaaaa atactttcgc tttccaatgc
      421 tgaaagaagg cgacactgcc gccaagcgcg acgcgatgcg cgcattcctt caccaacacg
      481 gttatcgtaa cggtcacgta acgatcgaca actccgactg gtacatcgat cagcggctaa
      541 ccgcacgcct aaaagacaat ccggcggccg acgttaagcc gtatcgcgat tactatctcg
      601 aacacatttg gtcgcgggcg atgtactaca acgctctcgc tcatcgcgtg gctggttatc
      661 aggtgaagca cacgatcctg gtacatttca atcttctcaa cgcactattt ctcggtgatg
      721 cgatcgcgat gttcaagtcg aaaggttggc aaccgatcga tgctgaagca gcatttacag
      781 atccggtttt cgaatctaag ccgaacgtca tccccgcagg cgaaagtatc atttggtcac
      841 tcgccaaaga gaagggaact ctcgcgaaat cgctgcgtta tccccccgaa gacagtgaat
      901 acgaggccgc gcgaatgaag aagttaggac tatgaaccga ctcttactct cgacctgcat
      961 attgctcgta atcacgagtt ccgttatggg ccaggtcagc gatgtcgcac cgacagctgc
     1021 cgctgaccca tcgtcatgga agcgatatac ggctatggat gaagcgtttt cgatcatctt
     1081 tccaaccgtt cccgcaatgg cgacgtcgag agaatacttc gaaaacggcc aaagagaacg
     1141 gcgcatacga gagattggcg cctacgctga cggaatcgtg tacaccgtat attcagtcga
     1201 gaatcccggg cctaaacagt cgctggagcg ctttatcaaa gaacgtttcg gcaacttcaa
     1261 gactcgagaa gtgactgtct cgggtgttgc cggtaaggaa cccgtcgatc aaaaaggcga
     1321 tttggtccgc aagtactttg ccaccgagcg tcatctttac gtatttagta ctcttggcgc
     1381 gcccgcatcg gatcccagag tccagcagtt tttctcctct ctcgttctgg gcaagaaccg
     1441 tgaaggcatt gaggtttccg atggtcctgg aatcactttg cagccgcccg ttcaagcgga
     1501 acctgtagat gctaacatcg cgccaaagtc gtacgtgggg cgagaggttg atcgaaaggt
     1561 tcgactgggc atgaagccgc agccgactta cacgaacgcg gcaagactga atgggttaac
     1621 cggtaccgtg gtgctgaagg tcattttttc ctccgccgga gaggtcaaaa acattcgaac
     1681 tgtttcgggt ctgccatatg gtctaacaga aaaggcgatc gatgctgcca gaaaaatcaa
     1741 gttcattccg gcagtgaagg agggaaagta cgtttccgta tggatgcaac tcgagtacaa
     1801 cttcaatctc ttctgatcac gaatgccgca cctactcgaa gtcaaagatc tccaaactca
     1861 cttcccgact cgcgcgggat tggtgcgtgc tgtcgacggc gtcagttttt atcttgatca
     1921 cggtgaactg ctcggacttg tgggcgagtc gggttgtggc aagtcgataa ctgcactttc
     1981 agtcatgcga ctgattgcgc cgcccggcag gatcgtcaac ggagagattc gttttctcgg
     2041 cactaatcta ctcgagcttt ctgacgccga gatgcgccgg atgcgtggcg acgacatcgc
     2101 catgatcttt caggatccga tgacgtcact caatccggtc ttcacggtcg gcgaacagat
     2161 cggcgaagcg ttgcgtctgc accggcagat gtcgcgcaaa caggcccgcg aggcaacagt
     2221 cgaagcgatg cgcgaagtgg cgattcccga tcccgctcgc cgcattaacg actatccaca
     2281 tcaactctcc ggcggtatgc gacagcgcgt gatgatcgcc atggcgctgg cgtgcaatcc
     2341 gaagttgttg attgcagacg agccgacgac cgcgctcgac gtaacaattc aggcacagat
     2401 tctcgagttg ctgaacgaat tgcgaaaaca acgcgatctc gcggtgctct tgatcacgca
     2461 cgatctcgga gttgttgctg aggtggcgga ccgggtggcg gtgatgtaca cgggacgcat
     2521 cgtggaagag tcgccggttg aggaactgtt tgcgcgtccg aagcatccct acaccgaggg
     2581 actgctgcgt tcggtgccaa agcttacgag cgaacacgtc gcaaaacgtg aacgtctcga
     2641 aaccatcgaa ggagtagttc ccagtccaac tgatttgccg cccggctgcc atttcgcgcc
     2701 gcgttgtccg catcgaatgc cgcgttgcac ggaagagagt attccgcttt accagctcga
     2761 aggcggcgtg caagtgcgtt gtgtgctttt cgatttggcg gctgccgttg cggcagacaa
     2821 gcagaccacg gattttaaga gtcagtaatg caacaggttc aagttcagga acaaacacgg
     2881 attttttcgc ccgctcgcga cgcgctggtg agcgtgcgcg ggctggtgaa acattttccg
     2941 gttgagggtt cggacgacgt agtacgcgct gtcgatggcg tttccttcga catcttcagc
     3001 ggcgagactt tgggactcgt cggcgaatcc ggctgcggca agtcgacggt tggacgctgc
     3061 ctgctgcgtt tgattgaacc aactgcgggt cagatcacct tcgagggaca ggacgtactc
     3121 tcactcgaca agaaacggct gcgccaactt cgacgcgaga tgcagatcat ctttcaggat
     3181 ccttacgctt cactgaatcc gcgcatgaaa gtcggcgaca tcatcgcgga gcctttagtc
     3241 attcacaacc agggcgccaa aagcgagcgc cgcgaacgtg tcgcttcgct gttgcgacga
     3301 gtcggactcg atcccaacta catgaaccgt tacgcacacg agttttccgg cggccagcgc
     3361 cagcgtatcg gtgttgcgcg cacactcgca ctcaacccga agttgatagt tgctgacgaa
     3421 cccgtctcgg cgttggacgt atcggttcag gcgcaggtcg tcaacctgct gcaggaactt
     3481 caggaagaat tcaaactcac ttacctgttt atctctcatg gcctcgcggt cgtagaacat
     3541 atttccacac gtgtggccgt gatgtatctc ggacgcattg tcgagattgc cggcgctgcc
     3601 gagttatacg cgcggccgct gcatccttac acgcaggctt tgttgtcggc gataccaatt
     3661 cccgatccgc gtcagaagcg aaagcgaatc atcttgcagg gcgacgtgcc gacgccgatc
     3721 aatccgccat cgggttgccg gtttcgcact cgctgtccac tggcgatcga ggagtgtgcg
     3781 cacatcgatc ccgagctgcg agaagtgtcg ccggaccatc acgtcgcgtg catacgcgtg
     3841 ccgggttggt ccgaagcccc ggcaagactg tgaaaccttg acccgcgtta gcgcgtatca
     3901 aaaccagtcc aacacagctt tctcatagga gaacacgaat gagcgctccc caggatccaa
     3961 acttccaggc gccaccgccg ccaccaaccc tggaagcaga accggaagct ccccgagcca
     4021 ccgaattgcg tccctttgca attggcgttt ttgtgctcgg attgatcttc gcaggcggcg
     4081 gaattgccag actgcttgct ggaggaatcg gcaccggagc ctcgctctgt ttcttcggcc
     4141 ttctgctctt cgcgctgagt ttcattcgca agccggcgct gcccgaaact gagcgcccgc
     4201 tgtcgtggtt tgagaaggtc acgggagttt tctacgaacc aacgcgcgtc tttcgtaact
     4261 tgcgcattca tccttactgg atcggggctt ttctcacgat ctgggtgctg accacgatct
     4321 actcgacggc gttcattcaa cgaatcacgc cagagcgcat cacggagtac acggtggaaa
     4381 aggtctcgga gatgggacca ccgtttgccc cgccaccaga agccattgac agaatccgag
     4441 tcgagcaact ggagtcgctc aagaatccgg tccaacgcct gggtgcagta ttgaagtcga
     4501 tggtcagtac gttcgtcttt ggcgcgattg cagcagccct ggcgctactc ggcattctgg
     4561 tgtttggtgg acggatcaac ttctggcagg cactggccgt caccttttat gctacgttgc
     4621 cggtggtggc ggtccagaag cttcttgggc tggtcattct atttttgaaa acgcctgacg
     4681 acttacatcc aatccttaac caggaaacta cgcttcagga taacctcgga gttctgatct
     4741 caccggcaga ccatccggtg atatttgtca tcgcgagctt tatcggactt acttcgtttt
     4801 acggactctg gctgcgcgct aaaggactca accagggagc aacgcgcgtc agcaaaggcc
     4861 aggcgtgggg tgtggcgatt acgttgtggt tcttgttgtt gatggtggtc gtgatcttta
     4921 ccgcgctgtt cccgggcttt atctcataag ccgcgggcca tcactcgtaa cgcagcgatt
     4981 caatcggatc gagacgggct gctttccacg ccggccaaag gccgaagatc aggccgatgc
     5041 cgacgctggc gatcagcccg ccgatcggtg cccatagcgg aacgtaggac ggaagtagca
     5101 tccgcagtag cagcgtcagt ccccaaccaa tcgacagacc gacgattcca ccgagacctg
     5161 tcagcgtagc cgcttcgatc agaaactgcc acatgatgtc gctgcggcgc gcgccgattg
     5221 ctttacgcac gccgatctcg cgcgtgcgtt cggtcactga caccagcatg atgttcatta
     5281 cgccgattcc gccaaccatc agacccacgg aagagatcac gaccatggca atagccagac
     5341 cggctgtgat cgaacggaag ttatcgagga tgctttcgga cgtttccatg ccaaagttgt
     5401 cgtttgcggc aaacggcacc tgacggcgca cgcgcaacag gtcgcgcacc tggtcctttg
     5461 cctcttccat catgcccgcc ttccctacgg caagaatgta gatgtcgtca gcattcggtt
     5521 ttaacttctg ggccaccgtg aacggcacgt agataacgtt gttctgatcg tcactgccgc
     5581 cgccgatgag aaactgttcg cgcttttcga gcacaccgac gacgcgaaac tcctgcgtcc
     5641 caatcttgat cgtctggccg acgggtgagc ctatcttgaa gaagtcgtcg gcgacttttg
     5701 aaccgacgac acagacttcc tggttcgtat cgttttctgt ttgcgtgaag aaacggccct
     5761 cggagatgac ctgcgtgcct gctttctcaa actccggcaa ggtgccttga agcgcgacct
     5821 gcgccgacgt cttacctcca cctgaaacca tcagtctgcc gccaaaccag ttattcgtga
     5881 tgtcgaggaa gggaaccgaa agttctactg tgggcaagga tcgtagcgcg atcgcgtcgt
     5941 cgtaagtcag ctccttgcgc attctttctt cgcgcgaccg gcggccgacg tgaataccag
     6001 gattgaactt actgatgtag atggaacgcg tgccaaacga ttcgacttcg cgtgtgacgg
     6061 agacgtcgat gccgctgatg atcgaggcaa tcaacatgac cgtgatgatg ccgatgacga
     6121 cgccgaacac cgtcaaaaac gagcgcagtt tgttggcgcg caaagtggcc agcgccatca
     6181 tgaggttttc gaaaatgtcg cttcgcagaa ttcgcatttt agtcagctct caacgcttca
     6241 atcggatcga gtctcgccgc cttccacgcg ggaaaaattc cggaagcaac gccggcgccc
     6301 cctgacgccg cgagtgctcc cgcaatagca agaatcgaca ggtaagtcgg aaagatccac
     6361 gcggtcacaa tcgcgccgac gatccacgca agaaagatcc cgacagcgcc gccgataacg
     6421 gctagcgtca ccgcctcgat cagaaactgt ttcagtatat cgatttgccg cgcgcctaaa
     6481 gattttctaa taccgatctc tttggtgcgc tcggtgacgg aaaccagcat gatgttcatg
     6541 atcacgatgg cgccgacgac cagcgcaatg ccgggcaccg cgatcgcgac gatgaagatg
     6601 gtgccaaaga gtcgatcgcg cagcccggtg atcgcatcgg gtgtcacgat gccgaagtta
     6661 tctttttcgc ttggtccgag tttgcgtctg gcgcgcatca ggaagcgtgc ttcttcgacc
     6721 gcgtcgttga agagttcgtc gcttcttgca gtcgcgacga aatagagcga gcgttgccgg
     6781 attagtccgc cgtagtttac ggagtaggtc ttcagcggaa tgataataaa gttgtcctgt
     6841 ggaatgccga agaccgtgcc cttggctgct tcgacgccga tcacgcggta aggcagaccg
     6901 cgaatcgaga tctcctggcc caccgaattg ccgccgggga agagtttgtc agcaatatca
     6961 gcgccgatgt aggcgacacg cacggcgccc tcgttttcag gttcggcgat gaaacgtccg
     7021 tcggcaacgt cgcggttctc gatgtcgacg cagttggcag tcgcacccga gacaaagatg
     7081 tcttcgagaa cttcttcacc gcgtttcact tctgacggag tgacacgggc gcgcgcgcca
     7141 atctggccga tgagcgcggc gcgttcttta atatagtagt agtcgccgag cgtgagctct
     7201 ttgttgcgtc gctgcgcagc agcgatcgtg tcggtatcct tgaagtcttc gagtggattg
     7261 aagcgctgga tcgtaaacga cttggcgccg atgcccgcga tcttttcgtc aacgtaacga
     7321 ttaaaaccct caatcaacga aacgacgacc acaaacgccg tgacgccgac aatcatgccg
     7381 agcagcgtca gcgctgagcg caatttatgc gcccagatag ccgcgatcgc gagtttgaga
     7441 gtctcgataa gtttcacaga gttactacag tataggtccg tttgttcctg gaccgtaggc
     7501 gtctcgactg cattcgtcga agagaactac tttcgtgacc tgcaggcgag acgcctgcag
     7561 ccccaggaaa aaagtcttca ctcccagcgg agggcgtcga tcggattcag gcgtgcggct
     7621 ttccacgccg gccagagccc gaaaacgatt ccgaccgccg cggagatccc gacaccgact
     7681 ccgatggccc atagaggaac gtacgccggt aacggggagt atttgttcat caacagactg
     7741 gcgacctcgc caaccgccag tcccgccaat cctccaatcg cggtcaacac cacggcttca
     7801 atcaaaaact gcgacagaat attcaacctc gtcgcaccga ccgccttgcg cacgccgatc
     7861 tccttggtcc gctctgtcac cgacaccagc atgatattca taacgccgat cccgccaatc
     7921 atcaaagcca cgaatgagat tgacgtcagc acgagagcgg ttgctccggt gagttggtta
     7981 taaacgtcga gcagcgaatc ctgcgaagag ataccgaagt tgtttggggc actcgccgga
     8041 acccgcctgc gccgccgcag tatgtcagtg atctcgtcca tcgcattctt cacaaatgcg
     8101 cgcgaacgcg gtcgcacgat cacgaaaaac acgacttcag ggaagggacg gtcaggataa
     8161 tacttggcga acgtcgttat aggaatgaaa attgagttgt cccgcgactg gccgaagaag
     8221 ttgcccaacg gctccatcac tccgatgatg cgatacggag tcccttcgat tcgcacttcc
     8281 ttgtcaactg cttcttcgta aggaaacagt gcctgcttca catcaacgcc gagaatgacg
     8341 acgttggaac gctcctgcat gtcgatttcg ctgatgaacc gcccgctgcc gacgtactgg
     8401 ctgtgaacga actcgtaata cggcgtgacg ccaaagagaa ccggcgtgtc tgtctgtttg
     8461 tccttgtaac gtatggtggc tgacaacagg cgctgaatcg gcgagacgcc ggcaatcgaa
     8521 ggcgcctctg tgcgcaatgc ttcggcgtct tccatcgtga ggtccttgcg gtggatctct
     8581 tccggcccgg gcgggcggcc aaaactcggc tcaaacttcg acacaaagat cgtatttgat
     8641 ccgagcgatt cgatctgatc cgcgaacgct ttgttcagcc cctgaatgat cgagaccatg
     8701 aagattacgg tcaccacgcc aacgctcaca ccgagaattg tcaacgacga gcgcagcttg
     8761 ttcgtgcgca acgtgtcgag cgccatcagc gccgattctt tgatgtcctc aaatctcatt
     8821 cggccctcag tgcttcaatt ggatccagct tggctgcgcg ccacgccggc cacaatccac
     8881 tcacgactcc cactatcgac gaaactccaa ctcccaatac
//
LOCUS       DASTEY010000068         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_594559, whole genome shotgun sequence.
ACCESSION   DASTEY010000068 DASTEY010000000
VERSION     DASTEY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594559"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /locus_tag="VFO72_01195"
     CDS             <1..2175
                     /locus_tag="VFO72_01195"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET9477927.1"
                     /translation="EDIVEKVAANHAQADLDLLRRAYLFSAKEHKGQKRASGESYLVH
                     PLEVANILADMKLDEVSVATGLLHDVVEDTLVDLETIRRYFGEEITHLVDGLTKIAQI
                     SNISREEQQAENVRKMLLAMIDDVRVVLVKLADRLHNMRTLQYLSPEKRKRIAEETME
                     IFAPIAHRLGMGKLRGELEDLAFRHLHPEDYRELAEQLEKRRPENEAFLKAVTARIEE
                     KMNEAEVPFVVVEGRVKRLYSIWKKLRAQKISLDQVYDLVAARIITPNEVRHCYAALG
                     VVHNNWKPVPGRIKDWIATPRDNLYQSLHTSVIGSNLQSFEVQIRTEDMHQIAEEGVA
                     AHWKYKEGKRGRREDDQALAALRSLVEWTQEVKDSKDFLDSLKLDLYPKDVYAFTPMG
                     KVIQLPRGATPVDFAYMIHSEVGNTCTGARINGRMVPLRTQIQNGDVVEIITTTNSHP
                     SRDWLNFIVTSRARNRVRHWVAEQQRTESIEIGRKLFEKEAARFQLSPKKVLSDTDKD
                     MKRVASDYGYGRVDDLLAAIGYGKLVPRNVIAKYLGPEQFEQLDKQKESRLRSGVRAV
                     KRLIRLGDDSILVRGIDDLMVTRARCCNPLHGEEIIGYVTRGKGVAVHTTRCKNVTQL
                     MVNRERIVEVEWAGRPETTAYAVKLLAITENRTGMIAGITGAISDMKTGIRDARASVA
                     SDERGHIEVTVEVFDVKHLDKVIGSIKGVPGVLDVERMQGAA"
BASE COUNT          531 a          605 c          687 g          433 t
ORIGIN      
        1 gaagatattg tcgagaaggt cgccgctaat cacgcgcagg cagacctcga tctactccgt
       61 cgcgcttacc ttttttccgc taaagaacac aagggccaga agcgcgcgtc gggcgagtcg
      121 taccttgttc acccgctcga agtcgcgaac attctcgccg acatgaaact cgatgaagtg
      181 tcggtcgcga cgggcctgct tcacgacgtg gttgaagaca cgctggtcga cctcgagacg
      241 atccggcgct actttggtga agagataacg caccttgtcg atggtctaac caagattgcg
      301 cagatcagca acatctctcg cgaggagcag caggcggaaa acgttcgcaa gatgttactg
      361 gcgatgattg acgacgtgcg tgtcgtgctc gtgaagcttg cggatcgact ccacaacatg
      421 cgcacgcttc agtatctgag tccggaaaaa cgcaaacgca tcgctgaaga gacgatggag
      481 atcttcgcac cgattgcgca ccggctcggt atgggtaagt tgagaggcga actcgaagac
      541 ctcgcgttcc gtcatcttca tcctgaagat taccgcgaac tcgccgagca actcgaaaaa
      601 cggcgtcccg agaatgaggc attcctgaag gcggtcaccg cgcgcatcga ggagaagatg
      661 aacgaagcgg aagtgccttt cgtggtcgtc gaaggacgcg tgaagcggct ctactcgatc
      721 tggaagaaac tgcgcgcgca gaagattagc ctggatcagg tttacgatct cgttgccgcg
      781 cgaatcatta cgccgaatga agtgcggcac tgctacgccg cgcttggcgt ggtccataac
      841 aattggaagc cggtgccggg tcgaatcaag gactggattg ccacgccgcg cgacaacctg
      901 taccagtcgc tgcacacgtc agtgatcggc tcgaacctgc aatcgttcga agtgcagatt
      961 cgcacggaag acatgcacca gatcgccgaa gaaggcgttg cagcgcattg gaaatacaaa
     1021 gaaggcaagc gcggccggcg cgaagacgac caggctttgg ccgcgctgcg atcgctcgtg
     1081 gagtggacgc aggaggtcaa ggactcaaag gattttctcg actcgctgaa gctcgacctc
     1141 tatcccaaag acgtctacgc gttcacaccg atgggaaaag taattcagtt gccgcgtggc
     1201 gcgacgcctg tcgatttcgc ttacatgatc cactcggaag tgggaaacac gtgcaccggc
     1261 gcgcgcatca acggccgcat ggtcccgttg cgtacgcaaa ttcagaacgg cgacgtggtc
     1321 gagattatca ctaccaccaa ttcccatccg tcgcgcgact ggctgaattt catcgtcacc
     1381 tcgcgcgcgc gaaatcgtgt gcgtcattgg gtcgctgaac aacagcgcac cgagtcgatc
     1441 gaaatcggac ggaaactgtt tgagaaagaa gctgcgcggt tccaactgtc gccgaagaag
     1501 gttctgagtg acactgacaa ggacatgaaa cgcgtggcta gtgattatgg ctacgggcgc
     1561 gtcgacgatc tgctggcggc gattggctac ggtaagctcg tgccgcgcaa cgtcattgcg
     1621 aagtatcttg ggccggaaca gtttgaacag ctcgacaagc agaaggaatc gcgcttgcga
     1681 agcggtgttc gtgccgttaa gcggctgatc cggcttggcg acgattcgat actcgtgcgt
     1741 ggcatcgacg acctgatggt gacgcgcgca cgctgctgca atccgcttca tggggaggag
     1801 atcatcggtt acgtgacgcg gggcaaaggg gtcgcggtcc acacgacgcg ctgtaagaac
     1861 gtgacgcagt tgatggtgaa ccgcgagcgc atcgtcgaag tcgagtgggc cggacggccg
     1921 gagacgacgg cgtatgctgt gaagcttctg gcaatcaccg agaaccgcac cggcatgatc
     1981 gcaggaatta ccggcgcgat ctccgacatg aagaccggca ttcgcgatgc tcgcgcgtcg
     2041 gtcgcatccg acgagcgcgg ccacatcgaa gtcacggtcg aagtgtttga cgtgaagcac
     2101 ctcgacaagg tcatcgggtc gatcaagggc gtgccgggcg ttttggacgt tgagcgcatg
     2161 cagggcgcgg cgtaaacgtt tccagcgaac cacagatgac acagattcat ttaattaatc
     2221 tgtgtaatct gtggatagaa aactgaaact aggata
//
LOCUS       DASTEY010000069         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_331094, whole genome shotgun sequence.
ACCESSION   DASTEY010000069 DASTEY010000000
VERSION     DASTEY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331094"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1520)
                     /locus_tag="VFO72_01200"
     CDS             complement(132..1520)
                     /locus_tag="VFO72_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HET9477928.1"
                     /translation="MRVLRLNGRTILLLTAEAVLVYGAMITAVYLRVGVEDAQFELVT
                     RQGYLKAAVACFFCLAAFYLFDLYDFLVMHDRRELVLRLIQALGLAWIALAFSFYMYP
                     ALMLGRGVSLIALPLALALMVLWRVSIHWFLGHPDFGERILIVGSGNLAVEVAREVLD
                     RPDAGYRIVGFVGTDADLLGKSLINPRVIGMTEQLDEIVKRENIDRIVVAMGERRGQL
                     PTNKLLQLSLAGDVTIEEGLSFYERITGRVSLNMLRPSWLIFTGRRRRAKIAELSRSM
                     VHWLVALIGAILSLPLVLLTAVLIKLESRGPVFYKQERVGKNGRTFVLMKFRSMRVDA
                     EQDGPVWASKGDSRTTRIGRIIRKIRVDEIPQFWNILKGEMSFVGPRPERPHFVAQLA
                     EEIPFYEQRHLIAPGLTGWAQIKYPYGASIEDARQKLQYDLFYIKNQGLLLDAVIIFE
                     TIKIILFGRGAQ"
     gene            complement(1517..>2282)
                     /locus_tag="VFO72_01205"
     CDS             complement(1517..>2282)
                     /locus_tag="VFO72_01205"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET9477929.1"
                     /translation="VTFDVSPSRVEPHLIAVSQPRSAYTEQFRSLRTKILEAGERMQM
                     RAFVVTSAGIAEGKTLTALNLAWLLAQTEGIRALVIDSDLRRPCATDYLGIDARLGLS
                     EVLGGQINLEDAIVRLQPSGLHLLPGGRPRDDVAELLSGPSYARILNEVRRMFDYIII
                     DAPPLGIFTDANVLMSKADAGLLVVRAGKTRYSVVDKLLEQMPKEKVLGVVLNRTQEQ
                     PDEAPYYYQHRYYNRERRTTEIQPGERLEEEVATVN"
BASE COUNT          502 a          685 c          641 g          454 t
ORIGIN      
        1 gtggttcggg tgcgtggctt ttgcggatcg tcctgcgtct gagccgcgct actcactgtc
       61 aacgcgatca acattagcgc agcgagagct acgcgtttgt atttagtcat ctccaacctc
      121 caacccttgc gttattgggc accgcgccca aacagaatga ttttgatcgt ctcaaatata
      181 atgacggcgt ccagcagcaa accctggttc ttgatgtaaa acaggtcgta ctgaagtttc
      241 tgccgcgcat cttcgatcga ggcgccgtag ggatacttga tttgcgccca accggttaat
      301 cccggcgcga tcaaatgccg ttgttcgtag aacggaatct cctcggccag ttgagccacg
      361 aagtgcggcc gttcgggacg cggtccgacg aagctcatct cgcccttcag gatgttccag
      421 aactgcggaa tctcatcgac gcgaatcttg cgaatgatgc gaccgatacg cgtcgtgcgc
      481 gagtcgccct tgctcgccca aaccggacca tcctgttcgg cgtcaactct catcgagcga
      541 aacttcatca gcacaaacgt ccggccgttc ttgccaaccc gttcctgctt gtaaaagacc
      601 gggccacgcg actccagttt gatcagcact gccgtgagca gaacgagcgg cagagaaaga
      661 atcgcgccga tcagtgccac gagccaatgg accatgctgc gcgatagttc cgcgatcttt
      721 gcgcgacgtc tgcgaccggt aaagatcagc cacgatggac gcagcatgtt caatgagacg
      781 cggccggtga tgcgttcgta aaacgacaag ccctcctcga tcgtcacgtc tcctgccagg
      841 ctcaactgca acaacttgtt ggtgggcagt tggccgcgtc gctcgcccat ggcaacgacg
      901 atgcgatcga tgttttctcg tttgacgatc tcgtcgagct gctcggtcat gccgatcaca
      961 cgcggattga ttaggctctt gcccagcaga tcggcgtcag tgcctacaaa gccgacgatg
     1021 cgataccccg catccgggcg atccaacacc tcgcgcgcga cttcgacggc gaggttgccg
     1081 gacccaacta tcaggatgcg ctcgccaaag tcagggtggc ccagaaacca gtgaatcgat
     1141 acgcgccaca aaaccatcag ggcaagcgcc agcggcagcg cgatcagcga gacgccgcga
     1201 ccgagcatca gtgccgggta catgtaaaac gagaaggcga gtgcgatcca cgccaggccc
     1261 agtgcctgga tcaggcgtag caccaactcg cgccggtcgt gcatgaccag aaagtcgtac
     1321 aaatcgaaaa gatagaaagc cgccaggcag aagaaacaag cgacggcggc tttcaagtat
     1381 ccctgcctgg tgaccagttc aaattgcgcg tcttctacac cgacgcgcag gtacacggcg
     1441 gtgatcattg cgccgtagac cagaaccgcc tcggcggtga gcaacaatat cgttcgcccg
     1501 ttgagacgta agactctcaa ttgacggtcg ctacctcctc ttcgagtctt tctcccggct
     1561 gaatttcagt cgtccttcgt tcacggttgt agtaacggtg ctggtagtaa tacggcgcct
     1621 catccggctg ctcctgcgtg cgattgagca ctacgcccaa cactttctcc ttcggcattt
     1681 gctccaacaa cttatcgacg actgagtaac gcgtcttgcc ggcacgcacc accagaaggc
     1741 cggcatcggc tttcgacatc aacacgttgg cgtcggtaaa gattccgagt ggcggagcat
     1801 cgatgatgat gtagtcaaac atgcggcgca cttcattcag aatacgcgcg taacttggtc
     1861 cggagagcaa ctcggcgacg tcgtcgcgcg ggcgcccacc gggcaacagg tgcagccccg
     1921 aaggttggag tcgcacaatg gcgtcttcca ggttgatctg tccgcccaac acttccgaca
     1981 agccgagccg tgcatcgatg ccgagatagt cagtggcgca gggacgtcgc aaatcgctat
     2041 cgatcacgag cgcgcgaatg ccttctgttt gggccaacag ccaggctaga ttgagtgccg
     2101 tgagggtttt gccctcggcg atgcccgcac tagtgacgac gaaggcgcgc atctgcatac
     2161 gctcgcccgc ctcgaggatc ttcgtacgca acgagcgaaa ctgctcggtg tacgcggaac
     2221 gaggctggct gaccgcaatc aagtgaggct cgacgcgcga tggggaaacg tcgaatgtga
     2281 cg
//
LOCUS       DASTEY010000070         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1303376, whole genome shotgun sequence.
ACCESSION   DASTEY010000070 DASTEY010000000
VERSION     DASTEY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303376"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFO72_01210"
     CDS             complement(<1..1056)
                     /locus_tag="VFO72_01210"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9477930.1"
                     /translation="MRVTSDDVVLFCRALREHGILVTPAEVVTAVAALRLIDTGDREE
                     VFLSLRSILAMRIEDFPIFAELFERFWSRRPMKIVVRESISPQRQVKGLAFFLEHWAS
                     STSVDSKPINLKGASDIESDAEKDFSGFSSDELQEISRLARRMVKRLARNPSRRWRPV
                     RRGRRLNLRRSLRLSLKTGGELIELAYKERRPKRTKLIVICDVSGSMDIYSRLLLQFV
                     YGLQNSFARVESFVFATSLSRITGELKNKTYQRALDRLSARVRGWSGGTRIGASLTAF
                     NLEWLRRVDKRTVVIVMSDGWDTGEPQQLALALSQLKKRAGRLIWLNPLLGSSNYQPL
                     ARGMQAALPFIDVFAPAH"
     gene            complement(1062..1940)
                     /locus_tag="VFO72_01215"
     CDS             complement(1062..1940)
                     /locus_tag="VFO72_01215"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9477931.1"
                     /translation="MKEEVRQIQRMLEEQVYITDAAVATALYLSLTLGKPLLVEGPAG
                     VGKTEIAKALANALATKLIRLQCYEGLDSTTALYEWNYPKQLLHVKLEEASPISEAEK
                     EAEIFSDAFLLKRPLLQAITEHERPPVLLIDEIDRSDEEFEAFLLEVLSDFQVTIPEI
                     GTIKATHRPHVVLTSNRSRDLSDALRRRCLYHWIDYPSLEKELEIVRAKVPALAASLA
                     MQVAKVMQATRTINLSKVPGVAETLDWANALVALQATELDNTIINETIGCFLKDETDI
                     ASFKMELEVGRFEAAD"
     gene            complement(1937..2536)
                     /locus_tag="VFO72_01220"
     CDS             complement(1937..2536)
                     /locus_tag="VFO72_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9477932.1"
                     /translation="MRKFISGLILGAGASQRLGPPKQLLPFRGGTMLGWIVDQAGRAT
                     GLDELVVVLGRAADQVRQQVDFGAARVVENPVFTEGCASSYRAGIAALDPRSAAIMII
                     LGDQPGIAPEIIDRAADEWRRNESQIALCSYQGRKGHPMIFARSMFEQLANLHGDKAA
                     WKLVDASHESVDEIRFDFPFPDDINTAQDYEQLLSAKTE"
     gene            complement(2533..3447)
                     /locus_tag="VFO72_01225"
     CDS             complement(2533..3447)
                     /locus_tag="VFO72_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9477933.1"
                     /translation="MNILDQCNELRARQEPFVLATVVAYKSPQSAKPGSKAIIKPDGS
                     TTGWVGGGCVQPIVLREAQAVLQTGKPKLLTITPDAGRDDWQGVESYRMACEGGGSLE
                     IYLEPVLPKPQLVIVGKSPVAETLSQLGELLDFKVIAANPDVARDQIDEDSFVVIATM
                     GNGDEEGLLSVLGTRPRYLGLVASEKKAKGLFAYAREKGATDEDIAVVKCPAGIEIGG
                     ETLPEIALSIIAEITRVRRTDIPVRSGRAGKPVPQNEATDPICGMTVTVENAKYTSVV
                     GGETFYFCCLRCKETFDRPQISQITSLS"
     gene            complement(3588..>3867)
                     /locus_tag="VFO72_01230"
     CDS             complement(3588..>3867)
                     /locus_tag="VFO72_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477934.1"
                     /translation="DESGNILQGSFMNYLVPTAVETPNWETGKTVTPSPHHPLGAKGV
                     GESATVGAPAAIVNAVVDALWHLGVRHIDIPITSAKVWKLLRDAGVRG"
BASE COUNT          844 a         1183 c          993 g          847 t
ORIGIN      
        1 gtgtgcggga gcaaacacat caataaaagg cagcgccgcc tgcataccac gcgccagcgg
       61 ttgatagttt gaactaccca acaatggatt cagccagatc aatctgcccg cgcgcttttt
      121 caactgcgag agcgccagcg ccagttgctg gggttcgcct gtgtcccacc cgtcactcat
      181 cacgatcacg acggtgcgtt tgtcgacgcg ccgcaaccat tcaagattga acgcggtgag
      241 acttgcaccg atgcgcgtgc ctcccgacca gccgcggacc ctggctgaca ggcgatccaa
      301 tgcgcgttgg tacgtcttgt tcttcagctc gcccgtaatt cgtgaaagtg aggtggcaaa
      361 gacaaacgac tccacacgcg cgaaactgtt ttgcagtcca taaacgaact gcagtaacag
      421 ccggctgtag atatccatcg acccggatac gtcacagatc acgatcagct tcgttcgttt
      481 tggacgccgc tctttgtaag ccagttcgat cagttcgccg ccggtcttta acgacaaacg
      541 cagcgaacgg cgcaagttca atcttcgacc acgccgcacc ggtcgccaac ggcggctcgg
      601 gtttcgcgcc agccgcttta ccatacgccg cgcgagacgc gaaatctcct gtagctcgtc
      661 actgctgaat ccgctgaaat ctttttcagc gtccgattcg atatcacttg cgcccttcag
      721 attgatcggc ttgctatcaa cacttgtcga actagcccaa tgttccagga agaacgcaag
      781 acctttaacc tgtcgctgcg gagagatcga ttctcgcacg acgatcttca tgggccgtcg
      841 actccaaaac ctttcaaaca actctgcgaa aatcggaaag tcctcgatgc gcatcgcgag
      901 aatgctgcgc aaactcagga acacttcttc acgatcgccg gtgtcaatca accgcagggc
      961 ggctacggca gtgacaactt ccgccggtgt aaccaaaatg ccgtgctccc gcaaagcacg
     1021 gcaaaacagc acgacatcgt cacttgtgac tctcatccgc gttaatctgc ggcttcgaaa
     1081 cgccccactt caagttccat cttgaacgag gcaatgtcag tctcgtcctt caaaaaacaa
     1141 ccgatagtct cgttaataat cgtgttatcc agctctgtcg cctgcaaagc aacgagcgcg
     1201 ttggcccagt cgagcgtttc tgcaactccg ggaaccttcg acaagtttat ggtgcgcgtg
     1261 gcctgcatga cttttgcgac ctgcatagcg agcgatgccg ccagcgccgg aactttggcc
     1321 cgcacgatct cgagttcttt ttcgagcgat gggtagtcga tccagtgata aagacagcgc
     1381 cgtctaagag catccgagag atcgcgcgag cggtttgaag tcaacacgac gtgcggccgg
     1441 tgagtggcct tgatagtgcc gatctccgga atagtcacct gaaaatcaga gagcacttcg
     1501 agcagaaacg cctcaaactc ctcatcgctc cgatcgatct catcaatcaa caaaacagga
     1561 gggcgctcat gttccgtgat cgcttgcagt agtgggcgtt taagcaggaa cgcgtcgctg
     1621 aagatttcag cttctttctc agcctcggaa atcggcgagg cttcttccag tttgacgtgc
     1681 aataactgct tggggtagtt ccattcgtaa agcgctgtcg tcgaatcgag gccttcgtag
     1741 cactgcaagc gaatgagttt agtggcgagg gcatttgcca gcgcctttgc aatctcggtc
     1801 ttgcccacac ctgccggtcc ctcgacgagt agcggcttgc cgagcgtcag cgacaagtac
     1861 aacgcggtcg cgacggccgc gtcggtgatg taaacctgct cttccagcat tcgctggatc
     1921 tgtcgcactt cttctttcac tctgtttttg cgctcagtag ttgttcgtag tcctgcgccg
     1981 tgttgatgtc gtcagggaag ggaaaatcga aacgaatctc gtcgaccgat tcgtgacttg
     2041 catcgaccag tttccaggcg gctttgtcgc cgtgaaggtt cgcgagttgt tcaaacatcg
     2101 atcgcgcgaa gatcatggga tgccctttgc ggccctgata cgagcaaagc gcaatctgag
     2161 attcgttacg gcgccactcg tcagcggcgc gatcgattat ttccggcgcg atgcccggct
     2221 ggtccccgag gatgatcatg attgcggctg aacgcggatc gagtgcagcg ataccggcgc
     2281 gatacgacga cgcacaacct tcagtaaaga ctggattctc gactactcgc gcggcgccaa
     2341 aatccacttg ctggcgcact tgatccgcag cgcgtccgag cacgacaacc aattcatcga
     2401 gacccgtagc gcgcccggcc tggtccacaa tccaaccaag catcgtgccg ccacgaaaag
     2461 gcaggagctg cttgggtggc ccaagccgtt gcgaagcgcc cgcgccgaga atcaatcccg
     2521 agatgaactt cctcatgaga gcgacgtaat ctgtgaaatc tgtggacgat caaatgtctc
     2581 cttacagcgc aggcagcaga aatagaatgt ttcaccacca accaccgaag tgtacttcgc
     2641 gttctcgacc gtcactgtca ttccgcagat cggatcagtg gcttcgttct gtgggacagg
     2701 ctttcctgcc cgtccagaac ggacaggaat gtctgtcctg cgcacgcgag tgatctcggc
     2761 gatgatgctc agagcaattt ccggcaatgt ttcgccgccg atttcaatcc cggcaggaca
     2821 cttaacgacg gcgatatctt catccgtggc gcccttctcg cgcgcgtagg caaacagccc
     2881 cttcgccttt ttctcgctcg ccaccagacc cagataacga ggtctcgtac caagcacgga
     2941 gagcagtcct tcttcgtctc cgttgcccat cgttgcgatt acaacaaaac tatcttcgtc
     3001 gatctgatcg cgagcgacgt ctggattcgc ggcgatgact ttgaaatcaa gcaactcgcc
     3061 aagctgcgag agtgtctccg cgacgggaga ctttccgaca ataaccagtt gtggtttcgg
     3121 caacacaggc tccagataaa tttcgagcga accgccgccc tcacaggcca tgcgatacga
     3181 ctcgacccct tgccaatcat cgcggcctgc gtcaggagtg atcgtcagca gctttggctt
     3241 acccgtttgc agcaccgcct gcgcttcgcg caacacgatc ggttgcacgc acccaccgcc
     3301 gacccaaccg gtcgtcgaac cgtcgggttt tatgatcgct ttcgatcccg gtttcgctga
     3361 ctggggactt ttatacgcaa cgactgtagc aagaacgaaa ggctcttgcc tggcgcgtaa
     3421 ctcgttgcac tgatcgagta tgttcacctc tatgccttcg tcgccgttgc ctgctgggac
     3481 cgcaggcgtc tcgcctgcaa ctcacggcaa cgtcctatcc gccattatgc aggcgagaca
     3541 actgcggtcc cagcaacaag gctctcaact gcatcagtcc tgttctgcta tccacgcacg
     3601 cccgcgtccc gcaacaactt ccaaactttc gccgaggtga tcgggatatc gatgtgcctc
     3661 acaccgaggt gccaaagcgc atcaaccact gcattcacga tcgccgccgg cgcgccgacc
     3721 gtcgccgact cgcccacacc tttcgcgccg agaggatgat gcggcgacgg cgtcacagtc
     3781 tttccagtct cccagtttgg cgtctcgacc gcagtcggca ccaggtagtt cataaaacta
     3841 ccttgcagaa tatttccact ttcgtcg
//
LOCUS       DASTEY010000071         4953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1402384, whole genome shotgun sequence.
ACCESSION   DASTEY010000071 DASTEY010000000
VERSION     DASTEY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402384"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..587)
                     /gene="lspA"
                     /locus_tag="VFO72_01235"
     CDS             complement(30..587)
                     /gene="lspA"
                     /locus_tag="VFO72_01235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9477935.1"
                     /translation="MNDKETRITRISIRERFGWRAGYLLAALGVYFADQASKAWAVKS
                     LRFGDERVLIPGFVKFFYTENPGIAFGQLQDGGNFGRWFFVVLAVAAAVAVFYYFLRT
                     PRNDDRVLGACALLLAGIVGNLTDRVRLGYVVDFIVLHAGNYHWPTFNIADSSITIGA
                     LLLAFDLIFSHRTKTEDVPGTVSHR"
     gene            complement(553..3555)
                     /gene="ileS"
                     /locus_tag="VFO72_01240"
     CDS             complement(553..3555)
                     /gene="ileS"
                     /locus_tag="VFO72_01240"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET9477936.1"
                     /translation="MSTETPLDLKKTVNLPQTGFSQKANLTQSEPARLQKWAQLDLYR
                     LLRQERAGRDKFILHDGPPYANADIHLGTAMNKILKDFIVKSRSMMGYDAPYVPGYDC
                     HGLPIEQHVERALAKKGKKKSDLSVESFRRACRDHAQNALKNQTRDFKRLGILGEWDD
                     PYLTMSNHYEAETARMFGRFVERGYVYKGARPVYWCIVDQTALAEAEVEYHQHTSPSV
                     YVKFPLRSDPALIDPALAGRKLFVLIWTTTPWTLPSNLGIAVHPDFEYSAFEHGDEVY
                     VVASELVEAVATKCALAAHDEEGQRVPPKVLARFAGAKLDRLEAGHPWLDRVSLLMVG
                     EHVTLGGEADAETELDVSEAQKKAPDTKAGTGCVHTAPGHGHDDFVIGKRYGLEIYCP
                     VDNAGRFTPDVEHFAGLSVFEANDKIVEFMNEKGVLLFSEKYEHRYPHCWRCKNPVIF
                     RATPQWFIAMDQVGGETSETGSLRAGAIAETERVNWIPAWGRDRMRNMLKARPDWCVS
                     RQRVWGVSIPVFYCAKCNEAVADPAVINHVADVFERESGDAWYTRAATELLPEGYTCK
                     ACGATEWTKETDILDVWFDSGTSSIAVLENREELRWPADVYIEGGDQFRGWFNSSLMV
                     GVAAHDRAPYQTVITHGWTLDAQGRAMHKSAGNAVEPDKVIKQSGAEIIRLWCASSNY
                     FDDMRASDEILQRVMDGYRKLRNTARFALGNLHGFDPAHDSVPVEEMEEIDRWALAEL
                     SAMIASIRKDYDAYEFHSVMQTVTSFSTVTLSARYFDIIKDRLYTYAPKSLGRRSAQT
                     ALYQIVDALARILAPVLAFTADEIWDHIPRSARDETPVSVHLALFPQPADYDAELLST
                     WKSLFELREIVLAELEKARSNKIIGSSLEARVVIKTSGEAYEFLKGYEPQLRYLFIVS
                     DAVEEVSDSDAVKGEALEIALKLQALAPFRTGGVQVRVERALGQKCERCWNYSTRVGE
                     FLRYPTVCERCIAALIEIERQGNTDYTD"
     gene            complement(3734..4306)
                     /locus_tag="VFO72_01245"
     CDS             complement(3734..4306)
                     /locus_tag="VFO72_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477937.1"
                     /translation="MTSLALQMSTRDPMFWVMVVVAASFIVIAIAMVAIAVFVNRAVK
                     SAGRMEEKMEPLIERVTFMSEQGKQIAVQGKLIAEQFSAVSSHLSTATLHFSESLAII
                     KGEVAELKALVSDTAVEARDKVELVSRTIDRTHRQVALTTDFIQTKVIEPARELAAIM
                     AGFRRGLEVLVAPVPKPINQTYGEDEMFIG"
     gene            complement(4397..4831)
                     /locus_tag="VFO72_01250"
     CDS             complement(4397..4831)
                     /locus_tag="VFO72_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9477938.1"
                     /translation="MGYDEYRRESGDGISTRLTYLLIGGGIGAILALLFAPKSGQELR
                     GDIADATRKGIDRSREAAQQLGDRAGEYYEATRTRAGELYSQAAEKVGEVAHTAREAA
                     SRQTGTVAAALEAGKKAYQEEKRKTELSGRIEASPNYGEKTN"
BASE COUNT         1063 a         1479 c         1309 g         1102 t
ORIGIN      
        1 ggaaaacgcc gtaggtgttg atggggaaat taccgatgcg aaacagttcc gggtacatct
       61 tctgtttttg ttctgtggct gaaaatcaaa tcgaacgcca gcaaaagcgc gccaatggtg
      121 atactcgagt cggcaatgtt aaaagttggc caatgataat tgccggcgtg caagacgata
      181 aagtcgacaa cataaccgag gcggacgcga tcggttaggt tgccgacgat tccggcgagc
      241 aacagcgcgc aagctccaag cacgcgatcg tcgtttcggg gagtacgcag aaaatagtaa
      301 aagaccgcga cagcggcggc aaccgcaagt acgacgaaga accaacggcc gaagttgccg
      361 ccatcctgta gctggccaaa cgctatgccc gggttttcgg tgtagaaaaa cttgacgaac
      421 cccggaatta gaacgcgctc gtcgccaaag cgcagcgatt tcactgccca cgccttcgac
      481 gcctgatccg cgaagtaaac gcccagtgcg gcgagcagat aaccggcgcg ccagccaaac
      541 cgttcgcgaa tactaatccg tgtaatccgt gtttccttgt cgttcaattt ctataagcgc
      601 agcgatacaa cgttcgcaga ccgttgggta acgtagaaac tcgccgacgc gagtcgagta
      661 gttccagcag cgttcgcatt tttgaccaag cgcacgctct actcgcactt gaacaccccc
      721 cgttctgaac ggagctaatg cttgcagttt cagagcgatc tctagagctt ctcccttgac
      781 tgcatctgag tctgaaactt cttcaactgc atctgagact ataaagaggt agcgcaattg
      841 cggttcgtac cccttgagga actcataagc ctctccactg gtctttatca ctacccgggc
      901 ctctaggctg ctcccgataa tcttgttcga gcgcgccttt tcaagttctg ccaagacaat
      961 ttcgcggagc tcaaataagg acttccacgt cgaaagcagt tcagcatcgt aatccgccgg
     1021 ctgtggaaac agcgcaaggt gtaccgatac gggtgtttca tcacgggcgc tacgcggaat
     1081 gtggtcccaa atctcatccg cagtgaacgc cagcacggga gcgagtatgc gcgcgagtgc
     1141 gtccacgatc tggtagagcg ccgtctgtgc ggaacgccgg ccgaggctct tcggtgcgta
     1201 cgtgtagagt cgatctttaa tgatgtcgaa ataacgcgcc gataacgtga cagtcgaaaa
     1261 actcgtcaca gtctgcatca cggaatgaaa ctcgtatgcg tcgtagtctt tcctgattga
     1321 cgcgatcatt gccgacagtt cagccagggc ccagcggtcg atctcctcca tctcttcaac
     1381 cggcacactg tcgtgcgccg gatcgaagcc atgcaaattc ccgagcgcaa accgcgcggt
     1441 gttgcgcagt ttccggtaac catccatcac gcgttgcagg atctcgtccg aagcgcgcat
     1501 gtcgtcgaag tagtttgacg acgcacacca caaacggatg atctccgcgc ccgactgttt
     1561 gatcaccttg tcaggctcga ctgcattgcc ggcagacttg tgcattgcac gaccctgcgc
     1621 atcaagcgtc cagccatgcg taatcacagt ttgataaggc gcgcgatcgt gggccgcgac
     1681 acccaccatc aacgacgaat tgaaccagcc gcgaaattga tcgccgccct cgatgtacac
     1741 gtcggcgggc cagcgcagct cttctcgatt ctcgagcacc gcgattgacg acgtaccact
     1801 gtcaaaccat acatcgagaa tatcagtctc cttggtccac tccgtcgcgc cacacgcttt
     1861 gcacgtgtaa ccctcaggca gcaactccgt cgccgcgcgc gtgtaccacg cgtcgcccga
     1921 ctcgcgttcg aaaacatccg ccacatgatt gatcactgcc gggtcggcaa cggcctcgtt
     1981 acacttcgcg caataaaaga caggaatcga aacaccccat actcgctggc gcgagacgca
     2041 ccaatcgggc cgcgctttca acatgttgcg catgcggtcg cggccccacg ccggaatcca
     2101 gttcacacgc tctgtttccg caattgctcc agctcgtaac gaacctgttt ccgacgtttc
     2161 gccgcccacc tggtccatcg cgatgaacca ctgcggcgtc gcacggaaga tcaccggatt
     2221 tttgcagcgc cagcagtgcg gataacgatg ttcgtacttc tcgctaaaaa gcagcacgcc
     2281 cttctcattc atgaactcga cgatcttgtc gttcgcttca aacacactta ggcccgcgaa
     2341 gtgttccacg tcaggcgtga agcgtcccgc gttgtcgacc ggacagtaga tctccaatcc
     2401 gtaacgttta ccgatgacga agtcgtcgtg gccgtgaccg ggcgcagtgt gaacacaacc
     2461 cgttcccgct tttgtgtcgg gcgctttctt ttgcgcttcg ctgacgtcca actcggtttc
     2521 agcatcagcc tcgccgccga gcgttacgtg ctcgccaacc atcaatagcg acacgcgatc
     2581 gagccacgga tgtccggctt ctaaccggtc aagtttcgca cccgcaaaac gcgcgagcac
     2641 ctttggcggc acacgctgac cctcctcgtc gtgcgccgcg agcgcgcatt tcgtggcgac
     2701 agcttccact aattcgctgg cgacaacgta aacctcgtcg ccgtgttcga aagcggaata
     2761 ctcaaaatcc ggatggaccg cgatgccgag attcgacggc agcgtccacg gcgtagtggt
     2821 ccagatcagc acaaacagct ttcgacccgc cagagccgga tcgatcaaag ccggatcgct
     2881 gcgaagcgga aatttgacgt aaaccgaagg gctcgtgtgc tggtgatact cgacctcagc
     2941 ttcggcgagc gccgtttggt ccactatgca ccagtaaacc ggacgcgcgc ctttgtagac
     3001 gtaaccacgt tcgacgaaac gtccaaacat ccgcgccgtc tcggcctcgt aatggttcga
     3061 catcgttaga tacgggtcgt cccactcgcc gagaataccg agccgtttga aatcgcgcgt
     3121 ctggttcttc aacgcgttct gtgcgtggtc gcgacaggcc cggcgaaaac tctcgacact
     3181 cagatcgctc ttcttcttac ctttcttggc gagcgcgcgt tcgacgtgtt gttcgatcgg
     3241 cagaccatga cagtcgtaac ccggcacgta cggtgcgtcg tagcccatca tgctgcgtga
     3301 tttgacgatg aagtccttta gaatcttgtt cattgccgtg ccgagatgga tatcggcatt
     3361 ggcgtacggt ggaccatcgt gcaggatgaa cttgtcgcgt cccgcgcgct cctggcggag
     3421 cagccggtag aggtccaatt gcgcccactt ttgcaaacga gcgggttcgc tttgcgtcag
     3481 attggctttc tgtgagaagc cggtctgcgg caagttaacc gtctttttta gatcgagcgg
     3541 ggtttctgtc gacataagcg taagtgctag atactagcaa agtcattgcc gattgccgat
     3601 tgccgattgc caattgccga ttgaagacct ccggccacga gaaatcggca attggcaact
     3661 cggcaataaa aaaagaaccc gagccatcga ggatgcgatg gttcgggttc taacgagccg
     3721 tttgcgtgcc tgcctaacct atgaacatct catcctctcc gtaggtctga ttaatgggct
     3781 tcggcactgg cgcaacgagg acctcgaggc cgcggcggaa gcccgccata atcgccgcca
     3841 actcgcgtgc cggctctatg accttcgttt gaatgaagtc ggtagtgagc gcaacttgcc
     3901 ggtgcgttcg atcgatcgtc cggctaacca gttcgacctt gtcacgcgcc tccacggcag
     3961 tgtcactcac cagcgctttc agctcggcga cctcaccttt aatgatcgcc agcgattctg
     4021 agaagtgcaa agtggcagtc gaaaggtgac tggagacggc gctgaactgc tcggcaatca
     4081 gttttccttg tacggcgatc tgtttgcctt gttcgctcat gaaggtgacg cgctcaatga
     4141 gcggctccat cttctcttcc attcgcccgg ccgatttcac ggcgcggttg acaaagaccg
     4201 cgattgccac catggcgatg gctatcacga taaacgacgc agcaacaacg accataaccc
     4261 aaaacatcgg gtctctggtg ctcatctgta gcgcaaggct agtcattgtc gattttcgat
     4321 ttgcgattgc cgatttataa gaccgagccg gctgctgttt caaatcggca atcggcaatc
     4381 gacaatcggc aatctcctag ttggtctttt cgccgtagtt agggctggct tcgatgcgac
     4441 cggacaattc agtcttccgc ttctcttcct ggtaagcctt cttgccggct tcgagtgcgg
     4501 cggctacggt tccggtttgc cgcgacgctg cttcacgtgc ggtgtgggct acttcgccca
     4561 ccttttcggc tgcttgcgaa taaagttcac cggcgcgagt gcgcgtcgct tcgtagtact
     4621 cgcctgcccg atcccccaac tgttgcgccg cttcgcgcga acgatcaatc cctttgcgcg
     4681 tcgcatccgc gatatcgcca cgcagttcct ggcccgactt gggcgcgaac agcagggcga
     4741 gaatggcgcc gatgccaccg ccgattagca gatatgttaa gcgcgtcgag atgccgtcgc
     4801 cgctctctct tcgatattcg tcgtatccca ttttgatggg tcctttcttc taaaagcgaa
     4861 gccctatgct tcgctggtct ctaaatatta cgaggcgttg gaatatagca cagattatcc
     4921 ggcaactgcc tactgccggc agctactcat ggc
//
LOCUS       DASTEY010000072         5059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1006854, whole genome shotgun sequence.
ACCESSION   DASTEY010000072 DASTEY010000000
VERSION     DASTEY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006854"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFO72_01255"
     CDS             complement(<1..308)
                     /locus_tag="VFO72_01255"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET9477939.1"
                     /translation="MKILFCFVMLLTAISFAMAQQEPGVTTDPIEQLRLTPDQRQAIR
                     KIVAETRDERQMSNRRVREANVALDQALDAEPADENLIEQRISELATAQAAQLRMRIH
                     T"
     gene            complement(317..961)
                     /locus_tag="VFO72_01260"
     CDS             complement(317..961)
                     /locus_tag="VFO72_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477940.1"
                     /translation="MNDRENFSTCERADELVSVLYGEATKRERGDFEAHLNQCATCRA
                     EFAAFAQVRQSIGEWRDEALIGFVFSRATAAPAKKSATGALRQFFDLSPLWMKGAVGF
                     AAVLFCVLAALALMRLQRVEKISPVATVNPDAIYTKQDLERAVLEALAKEQREPAPFI
                     TMVRPTPKRSNVAKVEPRSRRPFSKAEREQLAAELRLLSDDDVALDLLGDRINQ"
     gene            complement(967..1542)
                     /locus_tag="VFO72_01265"
     CDS             complement(967..1542)
                     /locus_tag="VFO72_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9477941.1"
                     /translation="METLGPDEIIVERALSGDAEAFGEIVRRWERRIFALTYGMLGRE
                     DDARDATQETFLAAFRSLRRFRGEAKVSSWLHRIAVNQCLTRQRRAKVRKEARLDEEE
                     SRANSFAAPLDYSPASVAEGKQRTAAVRVALNSLPLELRQVVVMKEFEELTFREISEA
                     LELPLSTVKSRLYTGLKQLQMRLAKFGGETG"
     gene            1734..3557
                     /locus_tag="VFO72_01270"
     CDS             1734..3557
                     /locus_tag="VFO72_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9477942.1"
                     /translation="MPEKLGRIRLLYIVLGVLLLVGLLPLAFAGLVLSGRSANELRSI
                     EGRYQAQLVQDKARQIELYGQRYRDVVTGLARAFEIAGGIQTFDNTGYDDRLEKTLQE
                     DPNLIALALWPVNGTLHRAFQTEVIKLEEVDARVSEVLARMSGRGLVVSGPQVIRSGQ
                     EMALTIAEPVLGGKNNQDVVAAVVAIVSFQEVFDVVQQPTSKSERELLNAGLPVVFVV
                     DQNGRAVAHPEASVAFSEKSMTDLKVVQDWQESGDQVQSALAPFSATRDGKTVDMLGS
                     YATAELDKTSRLGVIAIQDEAAALASVSDMRWQVLWISLVAGLLTILVGIFFANKLTH
                     PVSELASGAHRIASGDFSTRISVKSRTEIGDLGNSFNLMTDQIERYILDLQRSAEENR
                     ELFIGTVKALAAAIDGKDPYTRGHSERVSRFSMAIAQRLGMTDEEVEKIRISALLHDV
                     GKIAIDDNILKKPAALTDDEYEIMKRHPQKGYKIMSQIPAMKEFLPGMYMHHEMVNGM
                     GYPQGLKGDEIPLMGKIVAVADTFDAMTTDRPYQKAMKFEDALARIESFVGTRYDPAV
                     VAAFGEACREGQIRPGVVKLKKPQPASVPKPEPQMTQIGHV"
     gene            3571..4758
                     /gene="ftsY"
                     /locus_tag="VFO72_01275"
     CDS             3571..4758
                     /gene="ftsY"
                     /locus_tag="VFO72_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9477943.1"
                     /translation="MDIIHGFMGLFWRRKKEDEFVSLRLNEPSVKDAAPEVVPPEQPA
                     LEPPAKEAEPAPVPSRSTFSSSILGLDRSIEELQAQEAALEQEFSARFRRAVEATRES
                     LSERLDTVFAGRKQIDDELLDQLEEVLIAADIGVPTTLEILETVRRGIARKQINDLDA
                     LKQAIKDELLQILSASERQGVASETAVPESVAPYVMMIVGVNGVGKTTTIGKLAQRIK
                     AEGNDVLICAADTFRAAASDQLAIWAERTGVPLIQQKQGTDPAAVLFDSLKAAKARGS
                     DVLIVDTAGRLHTKSNLMAELEKMKRVAGREVEGAPHETLLVVDAVTGQNGLEQARQF
                     LKVAGVTGIVLTKLDGTAKGGIAVAIAKELNLPIRYAGIGEKVDDLVVFDPQQYVNSL
                     FGN"
     gene            4758..>5059
                     /locus_tag="VFO72_01280"
     CDS             4758..>5059
                     /locus_tag="VFO72_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9477944.1"
                     /translation="MTETDRRMMARALELAKEGVGQVSPGPLVGCVIVSSSGEVVGEG
                     FYIFEELNHAETIALATAGDRARGGTAYVSLEPHAHHGRTPPCTDALIAAGIKRVVA"
BASE COUNT         1105 a         1485 c         1428 g         1041 t
ORIGIN      
        1 gtgtgaattc gcatccgcaa ctgggcggct tgagcagtgg caagttcgct gattcgctgt
       61 tcgataaggt tctcgtcggc cggttctgca tcgagggcct ggtcgagcgc gacgttcgct
      121 tcgcgcacgc gccggttgct catctgtctt tcgtcccgag tttcagccac gatctttcga
      181 atcgcttgcc gttgatccgg cgtcaaacga agctgctcga tcggatccgt ggttactccg
      241 ggttcctgct gggccattgc aaaactaatg gctgtgagga gcattacaaa acagaacaaa
      301 attttcatct ctgcctctac tggttgattc ggtccccgag caggtccaaa gcaacgtcat
      361 catccgacag caatcgcagc tcggccgcca gttgctcacg ctccgctttc gaaaacggcc
      421 ggcgcgaccg gggctcaacc ttcgcgacgt ttgaacgttt aggagttggc cgcaccatag
      481 ttatgaacgg cgccggctcg cgttgctctt ttgcgagtgc ttccagcacc gcccgctcga
      541 gatcctgttt cgtgtagata gcgtcaggat ttactgtcgc cactggcgag attttttcaa
      601 cccgttgcag ccgcatcaat gcgagcgccg caagcacaca gaacagcacc gccgcaaatc
      661 cgaccgcgcc cttcatccac agaggcgaaa gatcgaagaa ctgtcgcaaa gcccccgtcg
      721 cagatttctt cgccggcgcg gccgttgcgc gcgaaaagac aaaaccaatc agtgcttcat
      781 cacgccattc accaatcgac tgacgcacct gtgcaaacgc agcaaactcc gcacgacagg
      841 tcgcacattg gttcaggtga gcctcgaaat cgcctcgctc acgtttggtt gcttccccgt
      901 agagcaccga aaccagttcg tccgcgcgtt cacacgttga gaagttttct cggtcgttca
      961 tccgcttcac cctgtttccc caccaaactt cgccaaacgc atttgcagtt gcttcaaccc
     1021 cgtatagagc ctgctcttta ctgtgctcaa cggcagctcg agcgcctccg aaatctcgcg
     1081 aaaggtcagc tcctcaaact ctttcatcac cacgacctgc ctcaactcca gcggcaaact
     1141 gttgagagcc actcgcaccg ccgccgttcg ctgtttgcct tcggcgacac tggccggtga
     1201 atagtcaagg ggcgccgcaa aactgttcgc tcgtgactcc tcctcgtcca gccttgcctc
     1261 tttccgcacc ttcgctcgcc gttggcgcgt aaggcactgg ttaacggcga tccggtgcag
     1321 ccacgacgag accttcgcct cgccgcgaaa cctgcggagg ctgcgaaacg ccgccagaaa
     1381 cgtctcctgg gtcgcgtcgc gcgcgtcgtc ttcacggccg agcatgccgt aagtgagcgc
     1441 aaagatgcgc cgctcccagc gccgcacaat ctcgccgaaa gcttcggcat ctccactcag
     1501 agcgcgttct acgataatct cgtccgggcc caaagtctcc atggcgctca actgtttaga
     1561 cgcgataccc cctcgaacag ccgatcaatc tgtgtttatc cgtggccaaa gtcgttgaca
     1621 cacaagtact tggtcgacaa taataacggc ggtccgagcg acgcgctcga acgaattcag
     1681 aaacctacca gaaacgcgaa gacctagcgc gattctaccg cctgacttaa cgaatgcccg
     1741 aaaaactcgg acgaatccgt ctcctctaca tcgttctcgg agttttgctc ctggtcggcc
     1801 tgctcccact cgcttttgcc ggtctcgtcc tctcgggacg cagcgccaac gagctgaggt
     1861 ccatcgaagg acgctatcag gcgcagctcg tgcaggacaa agcccggcag atcgaactct
     1921 acggccagcg ctatcgggac gtcgtcacgg gcctcgcgcg agctttcgag attgccggcg
     1981 gcatccagac cttcgacaac accggttacg acgatcgttt agagaagacc ctccaggaag
     2041 accccaatct tatcgccctg gcactctggc cggttaacgg aactctgcac cgcgcgtttc
     2101 aaacggaagt gattaagttg gaagaggtcg acgcgcgcgt cagcgaggtg ctggcacgca
     2161 tgagcggccg tggtctggtt gtcagcggtc cgcaggttat tcgctcagga caagagatgg
     2221 ccttgactat cgccgaaccg gttctcggcg gcaagaacaa tcaggatgtc gtggcggcgg
     2281 ttgtcgccat cgtgtcgttc caggaagtct tcgacgtcgt gcagcagccc acgtccaaga
     2341 gtgaacgaga actcttaaac gccggattgc ccgtagtgtt tgttgtcgat caaaacggac
     2401 gcgcggtggc gcaccccgag gcgagcgtcg cgttttctga aaaatcgatg acggacttga
     2461 aggtggtcca ggactggcag gaatcaggcg accaggtaca atccgcgctt gcaccattct
     2521 ccgccacgcg tgatggcaag acagtcgaca tgttgggatc ttacgcgact gccgaactcg
     2581 acaagacttc tcgactcggc gtgatcgcca tccaggacga agcggcagca ctcgcttcag
     2641 tttcggacat gcgttggcaa gtactctgga tcagcttggt tgccggactg ctgaccatac
     2701 tggtcggtat cttctttgcg aataaactca cgcatccagt cagcgagctg gccagcggcg
     2761 ctcatcggat tgcgtccggc gacttctcta cgcgtatctc ggttaagagt cgcactgaaa
     2821 tcggcgatct cggaaactcg ttcaacctga tgaccgatca gatcgaacgc tacattctcg
     2881 acttgcagcg ttcggccgaa gagaatcgcg aactcttcat cggtacggtt aaagcgctcg
     2941 cggcagctat cgacggtaaa gatccttaca cgcgtggtca ctccgaacgc gtatctcgtt
     3001 tctcgatggc cattgcgcag cggctcggga tgactgacga agaggtcgag aagatccgca
     3061 tcagcgcgct gttgcacgac gtcggcaaga ttgcaatcga cgacaacatt ctcaagaagc
     3121 cggcagcact taccgatgat gaatatgaaa tcatgaagcg gcacccacag aagggctaca
     3181 agatcatgtc gcaaattccg gcgatgaagg agttcctgcc cggaatgtac atgcaccacg
     3241 agatggtcaa tggcatgggt tacccgcagg gtttgaaagg cgacgagatt ccattgatgg
     3301 gcaagatcgt ggcggttgca gacacgtttg acgcgatgac gaccgatcgt ccctatcaga
     3361 aggcaatgaa atttgaagac gcactcgcgc gcatcgagag ctttgtcggc acccgttacg
     3421 atccggcggt ggtcgccgcg ttcggcgaag cctgtcgcga aggtcagatc aggccgggcg
     3481 tagtcaaact gaaaaaaccg caacccgctt cagtgccaaa gccggaacca cagatgacac
     3541 agattggtca cgtttgaatc tgtgtaatct gtggatataa tccacggctt catgggactt
     3601 ttctggcgca gaaagaaaga agatgagttt gtctcacttc gtttaaatga accttctgta
     3661 aaggacgctg ctcctgaagt agttccacct gagcagccag cgctcgagcc gcccgctaaa
     3721 gaagcagaac ccgctcccgt tccatcccgc tcaacattct cctcatcgat tctcggtctc
     3781 gatcgttcga tcgaagagct tcaagcgcag gaagctgcgc tcgaacagga attctccgcg
     3841 cgatttcgcc gcgccgtcga ggccacgcgc gaatcgcttt cagaaagact cgacaccgtc
     3901 tttgccgggc gcaagcagat cgacgacgag ttgctggacc aacttgagga agttttgatc
     3961 gctgcggaca tcggcgttcc gaccacactg gagattctcg aaacagtcag gcgcggtatt
     4021 gcccgcaaac agatcaacga tctcgacgcg ctgaaacaag ccatcaaaga cgaactgttg
     4081 cagatcctga gcgcgtctga gcggcaaggc gtcgcttcag agacggcggt acctgagagt
     4141 gttgcgccct acgtgatgat gatcgtgggc gtgaacggcg tgggcaagac gacgacgatc
     4201 ggcaagctcg cgcaacggat caaggcggaa ggaaacgacg tgctgatctg cgcggctgac
     4261 acgtttcgcg ctgccgcatc cgatcagctt gcgatctggg ccgagcgcac tggtgtcccg
     4321 ttgattcaac aaaagcaggg aaccgatccg gcggcggtcc tgttcgactc tttgaaagcc
     4381 gcgaaggcgc gaggttctga cgtgttgatt gtcgatacgg cgggccggtt gcacacgaag
     4441 tcgaacttga tggccgagct ggaaaagatg aagcgtgtgg cgggtcgcga agtcgaaggc
     4501 gcgccgcacg aaacgctgct ggtagttgat gcggtcactg gtcagaatgg gctcgaacag
     4561 gcgcgacagt tcctgaaggt cgcgggtgtc accggaatag tgctgacgaa actggacgga
     4621 accgccaaag gcggcatcgc cgtcgcgatt gcaaaagagt tgaacctgcc gatacgttac
     4681 gcgggtatcg gtgagaaggt tgacgatctc gtggtgttcg atcctcagca atacgtgaat
     4741 agcttgtttg gtaattaatg accgaaactg atcggcgcat gatggcgcga gcgctggagc
     4801 ttgcgaagga aggcgtgggc caagtcagtc ctggaccact cgtcgggtgt gtaattgtca
     4861 gttcttccgg cgaggttgtc ggcgaaggct tctacatctt tgaagagttg aaccacgccg
     4921 agacgattgc gcttgcgacg gccggcgata gggcgcgcgg cggcaccgcg tatgtttcac
     4981 tcgaaccaca cgcgcatcac ggccgcacgc cgccatgcac cgatgccctc atcgcggcgg
     5041 gaatcaaacg cgtcgtcgc
//
LOCUS       DASTEY010000073         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_397072, whole genome shotgun sequence.
ACCESSION   DASTEY010000073 DASTEY010000000
VERSION     DASTEY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397072"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="VFO72_01285"
     CDS             <1..1835
                     /locus_tag="VFO72_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9477945.1"
                     /translation="QFPSVWIAARIPYDVENRNNVQWRVIGRLKSGVSMPQAQAEAET
                     VAQKIREENTIAKTAGQYFQLVPMKQHLVDEVRPAILALMGAVIFLLLIACANVANLM
                     LVRASSRERELAVRAALGAGWWQLIRQTFAEALLLAALGTAIGVGLAYLGIRQLIAIA
                     PENLPRLNAIGIDMQVLAFSALAGLLSAVLFGVIPAIRTAKPNLMETLRAAGRTGTLG
                     AAGLRNAVVVVEVALAFVLLVGSGLMFRTFLNIQRVDMGFNPRGLLTFQLLGNIGNSP
                     QEFANFKRQLREQLDAIPGVNSVTASNPLPLAGGFNPVRWGGAEAQEDPSKFQAADLQ
                     IVLPGYFEAMGTRLLAGRTFTEEDSTPDRNLLIVDQALAAKAFPNESAVGKRILFRVR
                     TPEAQWGEIIGVVVHQRNTSLVEPGREQLYVTDGYVNHQAASWWALRTDGDPAALAGA
                     VREVVRNAGKETFINQMQPMDSRVVQAQAQTRFSLLLIGVFSTIAALLAGVGLYGVLA
                     TSVRQRTAEIGVRMALGAAPSRIFRLMVGKGLYLSMIGIASGLLAAFALTRVLASMLV
                     EVKPTDPITFVAVAVLFLFIAVMASWLPALRAAGLAPTTALRQE"
     gene            complement(2309..>3353)
                     /locus_tag="VFO72_01290"
     CDS             complement(2309..>3353)
                     /locus_tag="VFO72_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET9477946.1"
                     /translation="HGNDWPSGFAITELRARRRYDAFFQNTKTLFSLHNVGYQGLFDA
                     GDLWWLGFGEPPERDDFMLDRAASALKAGIIAADALSTVSPRYAREMQTHEQGHGLDW
                     LLRARRDRLVGITNGVDYTVWNPETDPHIAANFSVDDLSGKRECKIDLLRRFGLLEDA
                     NRPVLAIISRLVGQKGYDLIRTIADSILRTGPFFIALGAGAKEYEDFLQRWHDVAPKQ
                     VGIYKGYAGEPLAHQIEAGADMFLMPSLYEPCGLNQMYSMRYGTVPIVRATGGLDDTV
                     QQFDPSTGTGTGFKFGPYDAGALLEKIREALYFYTRPDDWQKIQRNGMLVDNSWTAAA
                     KKYLELYQDILKL"
BASE COUNT          680 a          946 c          965 g          762 t
ORIGIN      
        1 aacagtttcc gagtgtctgg attgccgcgc gcattcccta cgatgttgag aaccgcaaca
       61 acgtgcagtg gcgcgtcatc gggcgactca agtctggtgt ttccatgccg caagcccagg
      121 ccgaagctga aactgtcgcc caaaaaattc gcgaagaaaa cacgatcgcc aagacggccg
      181 gtcagtattt tcaactcgtg ccgatgaagc agcaccttgt tgacgaagtt cgacctgcga
      241 ttctcgcact catgggtgcg gtcattttcc tgttactgat tgcgtgtgcg aacgtggcga
      301 acctgatgct ggtgagagct tcatcgcgcg aacgagagtt ggccgttcgg gccgcactcg
      361 gcgcgggttg gtggcagtta attcggcaaa cattcgcgga ggcgctctta ttagcggcgc
      421 tcggaacggc catcggggta gggcttgcgt atttaggtat tcgccaactg atcgcgatcg
      481 ctccggagaa tctgcctcgg ttgaatgcga ttggaattga tatgcaggtg ttggcatttt
      541 cagcgttggc aggactgttg tctgctgtgc tctttggagt gattccggcg attcgcaccg
      601 cgaaaccaaa cctaatggaa acactgcgcg cggccggtcg aaccggcaca ctcggtgctg
      661 ccgggctgcg taatgctgtt gtcgtggtgg aagttgcgct tgcctttgta ctcctggttg
      721 gctcggggtt gatgttccgc accttcctta acattcaacg tgtggatatg gggttcaatc
      781 cgcgcgggct gctaacgttt caacttctcg gcaacatcgg caactcacct caggagttcg
      841 ccaacttcaa acgccagctt cgcgaacaac tcgacgcgat ccccggtgta aatagcgtta
      901 cagcgtcaaa tccattgccg ctggccggcg ggtttaatcc agtgcgttgg ggcggagccg
      961 aagctcaaga agatccgagc aagtttcaag ctgccgatct gcaaattgtt ctgcctgggt
     1021 atttcgaggc gatgggcacg agacttctgg ccggacgaac gttcacggag gaagacagca
     1081 cgccggatcg gaatctgctt atagttgatc aagcgctcgc ggcgaaggcg tttccaaacg
     1141 agtctgctgt cggtaagcga atcctgtttc gcgttcggac gcctgaggct caatggggag
     1201 agatcattgg cgtggtggtc catcagcgca acacgtctct cgttgaacca ggtcgcgaac
     1261 aactctacgt caccgacgga tatgtgaacc atcaggctgc gtcgtggtgg gcgttgcgga
     1321 ccgatggaga tccggcggcg ctggcgggcg cggtgcgcga ggtagtccgc aacgcgggca
     1381 aggagacgtt catcaaccag atgcagccga tggactcgcg ggtggtccag gcccaggcgc
     1441 agacgcgctt ttcgctcctg ctgatcggag tcttctcgac gattgcggca ctgcttgcgg
     1501 gcgtcggttt gtatggcgtg cttgctactt cggttcggca gcgaacggca gagattggag
     1561 tgcgtatggc tctgggtgcg gcgccatcgc gcatctttcg tctgatggta ggaaaaggac
     1621 tttacctcag catgattggc atcgccagcg gtttgctcgc agcgtttgca ctaacgcgcg
     1681 tccttgcgag tatgttggtg gaggtgaaac ccacagatcc gatcacgttc gtagcggtgg
     1741 cggtgctctt cttgtttatc gccgtaatgg cctcgtggct tccggcactc cgcgccgccg
     1801 gactggctcc gactacagcg ctgcggcagg agtagtggga gaggcggctt ctaatttaca
     1861 ccgtaaagga gatctcgccc gccactctag cgcgtagatt ttcgcttgtc atccgcttaa
     1921 tgcccaccag ctttgcctca aaagtcttct ttaattctca ccgggcttca gcccggtgtt
     1981 accgccacgc ggttgtgaga aaccgtttta acggtttcgc tggcgaatcc accgaaaacc
     2041 gttgaaacgg tttcctccgt ttcactgctc ctgcaccggg ctaaagcccg gtgagaatga
     2101 aagaagactt ttgaggcaaa gcacaagtca tcagtgcgtt tgccggcttc aggcacaaag
     2161 aaaccctaac agtaaagccc ctacgataaa ggacgtcctt tttgctggga ccgccggcgt
     2221 ctcgcccgca tggctcgtta gtgttcttct tcgctgcatt gcgggcggtc ccaggaacaa
     2281 ggcgtcccgg ctcgaggcgg tttccacgtc acagcttcag gatgtcctga tacagctcaa
     2341 ggtacttttt ggctgcggct gtccaggagt tgtctaccag cattccgttg cgttgaatct
     2401 tttgccaatc gtcaggccgg gtgtaaaagt aaagggcttc gcgtattttt tcaagtaaag
     2461 cacccgcgtc gtacggtcca aatttgaaac ctgtgcccgt tcctgtcgat ggatcgaact
     2521 gctgcaccgt gtcgtcgaga ccacccgtcg cgcgcacgat cggcaccgtc ccgtaccgca
     2581 tgctgtacat ctggttcaac ccacacggct cgtacaacga aggcatcaaa aacatatccg
     2641 cgccggcctc gatctgatgc gccaggggct ctcctgcgta acctttgtag atcccgactt
     2701 gtttcggcgc gacgtcgtgc caacgctgaa gaaagtcttc gtactctttc gcgcccgcac
     2761 cgagcgctat gaaaaagggg ccggttcgca gaatcgagtc ggcaatcgtg cgaatcaggt
     2821 cgtaaccctt ctgcccaacg agccgcgaaa tgatcgccag caccggtcga ttcgcgtcct
     2881 ccaacaatcc aaaccgacgt agcagatcga tcttgcactc ccgtttgcca gatagatcgt
     2941 ctaccgaaaa atttgccgca atgtgtggat ccgtttccgg gttccaaacg gtgtagtcga
     3001 caccgttagt gattcccacc agacgatcgc gtcgcgcacg caacagccag tcgagcccgt
     3061 gcccctgttc gtgcgtttgc atctcgcgcg catagcgcgg gctcacagtc gaaagcgcat
     3121 cggccgcgat gattccagct ttcagcgccg aagcagcacg atcgagcatg aagtcgtcac
     3181 gttcgggcgg ctcgccaaat cccagccacc aaagatcacc cgcatcgaac aatccctgat
     3241 aaccgacgtt atgcaacgag aaaagcgttt tggtgttttg gaagaacgca tcgtaacggc
     3301 gacgcgcacg caactcagtg atggcaaagc cgctcggcca atcgtttccg tga
//
LOCUS       DASTEY010000074         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_149725, whole genome shotgun sequence.
ACCESSION   DASTEY010000074 DASTEY010000000
VERSION     DASTEY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149725"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="VFO72_01295"
     CDS             <1..1817
                     /locus_tag="VFO72_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HET9477947.1"
                     /translation="EGTFLVDPSTPESRKYLKELFAKLASWGYEYFKIDGQPIVVREY
                     RNKKDFMKAPGDSDELYRDTLESIREAIGPDRYLLGCWVIPLEGVGLMNGSRIGADVL
                     PNWDGFKFAMRATMQYYFLHNVAWYADPDVFIVRAPLSLEQARAWVTLQGLTGQAALM
                     SDRLVDLSPERVELIKRVYPAVDIRPVDLFSSDRNKRIWDLKVNHLGREYDVVGVFNF
                     HESKSSSTYVSWQDLGLPEERPVHVFDFWNKEYLGAWEKGISVDLGPTRTSVLTLMPA
                     TDQIQLVSTSRHITQGWVDLISHSIRGNRYSGKSRLIKNDPYNLRFAFPRGKNFAIKS
                     ATAGGLPVRIVNHQGWAEVEITSSKTTDVSWEVLFEPTSGYHFPVKEPQHLWAESAGV
                     DGANLRWTIVHQPAAGYQVSLNGTVLGFTPSQVFALRGLDPNTTYTAEVRTVWQDGTL
                     SEKKAQLTFTLKKIQPPEMFLSDLDPVRLTPGWRQPELDRNFNSGGLSIGGRLFEKGI
                     GMPTNSEIEFELNGIYESFVALVGIDDEFNNNEAKAEFFVLGDGQELWKSDGLKKGDG
                     TKALKVEVKNVKRLMLRVRRVGEGGRVYADWVDAGLTK"
     gene            1814..>2294
                     /locus_tag="VFO72_01300"
     CDS             1814..>2294
                     /locus_tag="VFO72_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477948.1"
                     /translation="MISRRRILQLAVVSPLAAALPRLVGAQGISLREAPSAVVPLIED
                     ADVSRVKVERRWKGSVCQSHVVNQGTQAVRIKEVVLFDLVLSMPATTRLYGEGFQMLS
                     QTGGTLGQPTDLGNYTDAKHYKLAKAFYGMMMLSPSTRDNHLLAFTSCRRFIGQFYLS
                     "
BASE COUNT          488 a          557 c          713 g          536 t
ORIGIN      
        1 gggaaggaac gtttctcgtc gatccttcga caccggaatc gcggaagtat ttgaaagaac
       61 tgttcgcaaa gctcgcgagc tggggttatg agtacttcaa gatcgacggg cagccgatcg
      121 tcgtgcgcga gtaccgcaac aagaaagatt ttatgaaggc ccccggtgat tcggatgagc
      181 tttatcgcga cacgttggaa agcattcgcg aggcgattgg tccggatcgt tacctgctcg
      241 gctgctgggt gattccgctt gaaggcgtcg gattgatgaa cggatcgcgc atcggcgcgg
      301 acgtgttgcc gaactgggac gggttcaagt ttgcgatgcg cgcgacgatg cagtactact
      361 ttttacacaa cgtcgcctgg tacgccgatc cggatgtttt tatcgtgcgc gcgccgttgt
      421 cactcgagca ggcgcgcgcc tgggtgacgt tgcagggact caccggacag gcggcgttga
      481 tgagcgatcg tctcgtcgat ctctcgcccg agcgcgttga gctaatcaag cgcgtttatc
      541 cagccgtgga tattcgtccg gtggatttgt tttcatccga tcgcaacaag cgaatctggg
      601 atctgaaagt caatcatctc gggcgcgagt acgatgtggt tggcgtcttc aatttccatg
      661 aaagcaaatc gtcttcgact tacgtgtcat ggcaagatct cggattgcct gaggaacgtc
      721 cagtccacgt cttcgatttc tggaacaagg aatacctcgg cgcctgggaa aagggcatct
      781 ctgtagatct cggaccaacg cgcacgagcg ttctcacgct gatgccagcc accgatcaaa
      841 ttcagctcgt ctccaccagc cgtcacatca cacagggctg ggtcgacctg atctcgcaca
      901 gcatccgcgg aaatcgttac tccggcaaaa gcaggctgat caaaaacgat ccgtacaatc
      961 tgcggttcgc ttttccacgc ggtaagaact tcgctatcaa aagtgcgacg gccggcggat
     1021 tgccggtccg cattgttaat caccagggct gggctgaggt cgagatcaca tcctcgaaga
     1081 ccacggatgt cagttgggaa gttctttttg aaccaacttc cggttatcac tttccggtaa
     1141 aagaaccgca gcacctttgg gccgagagcg ccggggtcga tggcgcgaac ctgcgttgga
     1201 ccatcgtgca tcagccggcg gcgggttatc aggtttcgtt gaacggaacg gttttggggt
     1261 tcacgccttc gcaggtattc gcattgcgcg ggctcgatcc gaacacgact tacacggccg
     1321 aagtcagaac ggtctggcag gacgggacgc tcagtgaaaa gaaagcgcag ctcacattca
     1381 cactcaagaa gattcaaccg ccggagatgt ttctttccga tctcgatccg gttcgtttga
     1441 caccgggttg gcggcagccg gagctcgatc gcaacttcaa cagcggtggc ctctcgatcg
     1501 gtgggcggct ttttgaaaaa gggatcggga tgccaacgaa ttctgagatt gagtttgagt
     1561 tgaatggtat ctacgagagt tttgttgcgc tggtggggat tgacgacgag ttcaacaaca
     1621 acgaagcgaa ggcggagttt tttgtgcttg gggatgggca ggagttgtgg aagagcgacg
     1681 ggttgaagaa gggtgacggg acgaaggcgc tgaaggttga ggtgaagaat gtgaagcggt
     1741 tgatgctgcg cgtgcggcgt gttggtgagg gtgggcgcgt ttatgctgac tgggttgatg
     1801 cgggattaac caaatgattt ctcgacgaag aattcttcaa ttagcggtcg tttcgccatt
     1861 agcggcggcg ttgccgcgat tggttggagc gcaggggatt tcgttgcgtg aggcgccgag
     1921 tgctgtggtt ccgttaatcg aagacgcgga tgtttcgcga gtgaaggttg agcggcgctg
     1981 gaagggatct gtttgccagt cacacgtggt gaatcaggga acgcaggcgg tcagaatcaa
     2041 agaggttgtg ttgtttgacc ttgttctgtc gatgccggcg acgacgcgct tgtatggcga
     2101 aggctttcag atgctctcgc agaccggcgg cacgttgggc caacccaccg atctaggcaa
     2161 ttacaccgac gccaaacact acaagcttgc aaaagcattc tacggaatga tgatgctgtc
     2221 gccttcgacg cgtgacaatc acctgctggc gttcacttcg tgtcgccggt ttatcgggca
     2281 gttttatctg agct
//
LOCUS       DASTEY010000075         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_446371, whole genome shotgun sequence.
ACCESSION   DASTEY010000075 DASTEY010000000
VERSION     DASTEY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446371"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFO72_01305"
     CDS             <1..574
                     /locus_tag="VFO72_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477949.1"
                     /translation="YTGDRTKLTEAGLKTMLAHSISDLELEAAVEGLDAGHLLLIIDA
                     CNSGQALEAEEKRRGPMNSKGLAQLAYEKGMYILTAAQSFQAALEAAQLGHGYLTYAL
                     VEEGLKTPVADNEPKDGVLMAREWLNFATERVPQMQQEKMTQGRGLGLEIAFTEGEAD
                     ISDPQKRSVQRPRVFYRRELETTPLVIARP"
     gene            645..1223
                     /locus_tag="VFO72_01310"
     CDS             645..1223
                     /locus_tag="VFO72_01310"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9477950.1"
                     /translation="MSTKVEATIEDLYKVEGKAELVNGEIVHMSPTGGLPHYAAQQIT
                     ISLWFYQKATGIGFAIGDNSAFVVNLPHRKSFSPDAAFYSGKLTMKFSEGAPIFAVEV
                     RSDGDYGPRAERQMAQKRADYFAAGTLVVWDVDLLSDDVVRVYRATAPTTPVVYRRGQ
                     LAEAEPALPGWTMPVDDLFLKEQEAKHPITDG"
     gene            complement(1220..>2409)
                     /locus_tag="VFO72_01315"
     CDS             complement(1220..>2409)
                     /locus_tag="VFO72_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9477951.1"
                     /translation="AAGTGLIRTPFANAAAAVLPKGPAKKVLVLGAGLAGLVAAYELT
                     QLGHDVTVLEARTRPGGRVHTLREPFSDGLYAEAGAARIPNDHDLTLKYVRTFDLPLE
                     PFYPSRLNAIRFNGGHRQEVPIAGFTEAMTQNYGADLGGTPEHWQQIKGGTDLLPHAF
                     ARHLDKKIIYGAPVVRIEQDATAARVVFAKGGATQTLSADAVLCTIPFSVLGKLELPA
                     LSGRKRDAIKRTRYDAVSRVYLQTKNRFWEARGLNGFALTSGGIEIWQPTWNQPSPRG
                     ILMTYARPPEGERITKLKEHQRIETTLKQVEAIFSGLRGNFERGTTKCWLEDEWSRGA
                     WAFVGFTDFATAIVPDGRIHFAGEHLSQWFSWMQGALSSGLRAVKEIDEAQYPAAAKA
                     IRA"
BASE COUNT          542 a          680 c          701 g          486 t
ORIGIN      
        1 ctacacgggc gatcgcacga agttgacgga agcaggactg aagacgatgc tggcgcacag
       61 catttcggat ctcgagttgg aagctgcggt cgagggactt gatgcgggtc acctgttgct
      121 gatcattgac gcgtgtaatt cggggcaggc gttggaagct gaggagaagc gtcgtggtcc
      181 aatgaactcg aaagggctgg cgcaactcgc gtacgaaaag ggtatgtaca tacttacggc
      241 cgcgcagagt tttcaggcag cactcgaagc ggcgcaactg ggccatggat atttgacgta
      301 cgcgttggtt gaagagggtt tgaagacgcc ggtggcggac aacgaaccga aagacggtgt
      361 gctgatggcg cgcgagtggc tcaacttcgc caccgaacgc gtgccgcaga tgcagcagga
      421 aaagatgacg caaggccgcg ggttgggatt ggagatcgcg ttcacggaag gtgaagcaga
      481 catcagcgat ccgcagaaac gaagcgtgca gcgcccgcgc gttttctacc gccgcgagtt
      541 ggaaacgaca ccgttggtga tcgcgcggcc gtaacccagt tgcgttacgt actgcttggg
      601 gttaccatgc gatcacgagg taacaaaaaa tactcacaac gcctatgagc actaaagtcg
      661 aagccacaat tgaagatctc tacaaggtcg agggcaaggc tgagctcgtc aacggagaaa
      721 tcgtacatat gtcacctacg ggaggtttac ctcactatgc cgcccagcaa atcaccatat
      781 ccctgtggtt ttatcaaaag gctacaggaa taggctttgc tatcggagac aactccgcgt
      841 tcgtagtcaa ccttcctcat cgaaaatcat ttagccctga cgccgccttc tactcgggta
      901 aacttacgat gaagttcagt gagggcgcgc ccatcttcgc agtagaagtt cgcagcgacg
      961 gcgactacgg accgcgcgca gagcgacaga tggcgcagaa gcgcgctgac tacttcgctg
     1021 ctgggacact tgttgtttgg gacgtggacc tactaagcga tgacgtagtg cgcgtctacc
     1081 gagctaccgc accgaccaca cccgtcgttt accgaagggg ccaacttgca gaggcagaac
     1141 cggcgctgcc cgggtggacc atgccggtcg acgacttgtt tctaaaagag caagaagcta
     1201 agcatccaat cactgacggc taagcgcgaa tggcttttgc agccgcggga tactgcgctt
     1261 cgtcgatctc tttcactgct ctcaaccctg acgacagcgc gccctgcatc cacgaaaacc
     1321 actgcgagag atgctcgccg gcaaaatgaa tccgcccgtc aggtacgatc gccgttgcga
     1381 aatcagtgaa acctacaaac gcccacgctc cgcgcgacca ctcatcttcc aaccagcact
     1441 tcgtcgtgcc gcgctcaaag tttccacgca agcctgaaaa gatggcttca acctgtttca
     1501 gcgtcgtctc gatacgctga tgctctttca actttgtgat ccgctcccct tcaggcggac
     1561 gcgcatatgt catcagaatc ccgcgtgggc tgggttggtt ccacgtcggt tgccaaatct
     1621 cgatgcctcc actcgtcaac gcgaatccgt tcagtcctcg tgcttcccaa aagcgattct
     1681 tcgtttgcaa gtaaacgcgc gacactgcgt catagcgagt tcgcttgatt gcgtctcgtt
     1741 tgcgaccaga cagcgccggc aactcgagtt tgcccaaaac ggagaacgga atcgtgcaga
     1801 gcacggcgtc ggcgcttaag gtttgcgtcg cgcctccctt tgcgaaaact acccgcgccg
     1861 cagtggcgtc ctgctcgata cgcacgacgg gagcaccgta aatgatcttc ttatccaggt
     1921 gtcgagcgaa agcgtggggc aacagatcgg tgccgccttt gatctgttgc cagtgctccg
     1981 gggtaccacc caggtcggca ccataattct gagtcatggc ttcagtaaaa ccggcgattg
     2041 gcacctcctg tctgtggcca ccgttaaagc gaatggcatt gagccgtgat ggataaaacg
     2101 gttcgagcgg cagatcaaac gtccttacat atttgagtgt caggtcgtgg tcgttgggaa
     2161 tacgggcggc gccggcttcg gcatacagcc catcggaaaa aggttcgcgc aacgtatgaa
     2221 cgcggccgcc cggacgcgtg cgcgcttcga gcacggtgac gtcgtgtccg agttgtgtga
     2281 gttcgtaagc agcaacaagc ccggccagtc cggcgccgag aacgagcacc ttcttggccg
     2341 ggcctttagg tagcacggcc gccgccgcgt tcgcgaaagg cgttctgatc aggccggtgc
     2401 ccgcggctg
//
LOCUS       DASTEY010000076         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_446376, whole genome shotgun sequence.
ACCESSION   DASTEY010000076 DASTEY010000000
VERSION     DASTEY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446376"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFO72_01320"
     CDS             complement(<1..1304)
                     /locus_tag="VFO72_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9477952.1"
                     /translation="MHPSALAKRLYILLFVVIIVLYFYGLGSLPLLGPDEPRYAQVAR
                     EMFMRGDPVTPTLGGHTWFEKPALLYWLMMAAFKIFGVSEWSARFGPAVCGLLTIAAV
                     WLVGRAVGRASDQARDFSFWAVLITSSSLGLIVFSRAASFDVVVTMTTTWALAFFLLH
                     ELKHERKTAFLIGFYIFVGLSLLAKGLVGIVIPFGVVGLYYLLRRKWPARNLWLSLIW
                     GLPLAIAVSAIWYGPVIARHGWPFVDEFFIQHHFARYVSNKYHHPQPLYFYPAIILML
                     AVPWTAFLIEALAKIRSWGWRDNDPLNVVRVFSLAWLAMPIVFFSFSGSKLPGYILPV
                     LPAAALLIADPFMYSVSRWPIWATGVICLALAVGGMVYAFQSGHISLRCAALIAVPMV
                     VAAVASFMTRSERLVFGTVLFGATVVLVMFVLICAAQPVAQR"
     gene            1352..2344
                     /locus_tag="VFO72_01325"
     CDS             1352..2344
                     /locus_tag="VFO72_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9477953.1"
                     /translation="MSLRTAVIGAGSLGRQHARIHAALAIEGSSQFVAVCDLNEETAR
                     KVSEERNTGWTTDWRELIGRVDAVSLAVPTEVHCEIACGLLDAGIHVLVEKPISRTLD
                     EADRMIRAAEKGNALLQVGHLERFNPAMVALRPHVKKPVYFEIHRVGQFTARSLDIDV
                     VLDLMIHDLDIVQWLVGEEVEVTNIHAVGIAILTNKVDAANARLEFSSGAVANITASR
                     VGTEKIRKMRFFQPHDYVAVDYTTKRASVSRLAPPTATGSWPGVHIQHLDVIDVEPLR
                     AEIVSFLDSAENGLPPAVSGSDGRNALSLALRTLERIREHAEHPGVSELSANVT"
     gene            2356..3483
                     /locus_tag="VFO72_01330"
     CDS             2356..3483
                     /locus_tag="VFO72_01330"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9477954.1"
                     /translation="MVTRSEKLSSFLTEHIDSGEFPSAVYLAGEGDEIVFADALGHSV
                     VQPYRIANKLDTIYDLASLTKPLVTGMLCARRIELGELTLDSSVSHYLPEFERTDKQM
                     ITVRELLTHTSGLPAWRPLYILAEDEPERAAGAIANLDLEYKPGTRVVYSDLGFIALG
                     ILLERLTGQRLADMAQREIFEPLKLQQTFFNPELALQTGIAACETGNAYEMEMCVETG
                     SGAYQNSRQRLIWGEVHDGNAYFLGGAAGHAGLFSTAGDVFQLAQQFIAGFSQLLTAE
                     TCKLFRQNMTEGLDEARSLAWQLAATKDSTAGIDLPPDSFGHNGFTGTSLWIDAEHRR
                     VFILLTNRTHSRPLPFANINAVRREFHSLAIAEISKLSPAH"
BASE COUNT          869 a         1065 c          957 g          660 t
ORIGIN      
        1 tcacgttggg ccaccggttg cgccgcacag atcaacacaa acataaccaa aacaaccgta
       61 gcgccaaaga gcactgtgcc aaataccagg cgctcactgc gcgtcataaa cgaagcaacc
      121 gctgcgacga ccatcggcac agcaatcagc gcggcgcaac gcagcgagat atggcccgac
      181 tgaaaagcgt aaaccatccc gccaaccgcc aacgcaagac aaatcacgcc cgtggcccag
      241 atcggccagc gcgaaactga gtacataaaa ggatccgcga tcagcaacgc agcggcgggc
      301 agtacgggca ggatgtaacc cggcagtttc gatccggaaa aagagaagaa cacgattggc
      361 atcgccaacc atgccaacga aaacacccgc acgacgttca gcgggtcgtt atcgcgccag
      421 ccccaactcc ggatcttcgc cagcgcctcg atcagaaacg cggtccacgg caccgccagc
      481 atcaaaataa tcgccggata aaaatagagc ggctgcgggt ggtgatactt gttcgagaca
      541 taccgcgcga aatgatgctg gataaagaac tcgtcgacga acggccaccc gtgccgcgcg
      601 atgactggtc cataccagat cgccgatacg gcaatggcca acggcagtcc ccaaatcaaa
      661 ctcagccaga ggttgcgggc gggccacttg cggcgcaaaa gatagtaaag cccgacaacg
      721 ccgaacggta tcacgatccc gacgagacct tttgcgagta gcgacaggcc gacaaaaata
      781 taaaacccga ttaagaacgc ggttttcctt tcgtgtttta attcgtgtaa aagaaaaaaa
      841 gcgagcgccc acgtcgtcgt cattgttact acgacgtcga aactcgccgc gcgcgaaaac
      901 acgatcaggc caagcgaact cgacgtaatc aacaccgccc aaaaactaaa gtcccgcgcc
      961 tgatccgaag cacgcccaac cgcgcgtccg acaagccaaa cagcggcaat agtcaacagt
     1021 ccgcaaaccg ccggaccaaa tcgcgcgctc cattcactga caccaaagat cttaaacgcg
     1081 gccatcatca accagtaaag cagcgccggt ttttcaaacc acgtgtggcc cccgagcgtt
     1141 ggcgtgaccg gatctccacg cataaacatc tcgcgcgcca cttgcgcgta acgaggttcg
     1201 tccggtccaa gcaagggcag gcttccgaga ccgtagaagt aaaggacgat tatgacgacg
     1261 aagagaagaa tgtagagtcg cttggcaagc gcagaaggat gcaagtatat tcggctccga
     1321 ttcaaattaa cccggctaag ttacctaaac catgtcatta cgtacagcag tcatcggcgc
     1381 cggcagtctt ggtcgccagc acgcgcgcat tcacgctgcg ctggccatcg aaggatcatc
     1441 gcagttcgtc gccgtctgcg atcttaacga ggaaacggcg cgcaaagtca gcgaggaaag
     1501 aaacactggt tggaccaccg actggcgcga gctgatcgga cgcgtcgacg cagtgagcct
     1561 tgccgtgccg acggaagtgc actgcgagat cgcttgcggt ttgctcgatg cgggcattca
     1621 cgtgctcgtt gagaaaccga tctcgcgcac gctcgacgaa gccgatcgca tgattcgcgc
     1681 ggctgaaaag ggcaacgccc tgttgcaagt tggacacctc gagcgcttca acccggcgat
     1741 ggtagcgttg cgtccgcatg taaaaaagcc cgtttacttt gagattcatc gcgtcggaca
     1801 gtttactgcc cgctcactcg acatcgacgt cgtgctcgac ctgatgattc acgatctgga
     1861 tatcgtgcaa tggctggtcg gtgaggaagt ggaagtaacc aatattcacg cagtcggcat
     1921 cgcgattctg accaacaaag tagatgcagc aaatgcgcgt ctggagtttt cctcgggggc
     1981 cgttgccaac atcaccgctt cgcgcgtcgg cacggaaaag attcgcaaga tgcgtttctt
     2041 ccagccgcac gattacgtcg ccgtggacta cacaaccaaa cgcgcgtcag taagccgtct
     2101 tgcgcccccg actgccaccg gttcgtggcc gggcgtgcac atccaacatc ttgacgtgat
     2161 cgacgtagag ccgctgcgcg cggagatagt ttcatttctc gattcggccg agaacggttt
     2221 acctcccgcc gtgtctggat cagacgggcg aaacgcgtta agtttggcgt tacgcacgtt
     2281 ggagagaatt cgcgaacatg ctgagcatcc cggcgtcagt gaactcagcg cgaacgtcac
     2341 gtagttttca gttcgatggt cacgcgcagc gaaaaacttt cgagtttcct caccgagcac
     2401 atcgactccg gcgagtttcc ctcggcagtc tacctggcgg gcgaaggcga tgagatagtc
     2461 ttcgcggacg cgttgggcca ttcggtggtt caaccttatc gcatcgcgaa caagctcgac
     2521 actatctacg atctcgcctc gctgacaaaa ccactcgtaa ccggcatgct ctgcgcgcgc
     2581 cgcatcgaac tcggcgaact gacgctcgac agctccgtct cgcattacct tccggaattc
     2641 gaacgcacag acaaacagat gatcactgtg cgcgagttgt tgacgcacac ctccggtctt
     2701 cccgcatggc ggccgcttta catacttgcc gaagacgaac cggagcgtgc cgccggtgcg
     2761 attgccaatc tcgatctcga gtacaaaccc ggcacgcgcg tcgtttacag cgatctcggt
     2821 tttatcgcgc tcggaattct cctcgaacgt ctgaccggtc agcgtctggc ggacatggcc
     2881 caacgcgaga tcttcgagcc gttaaaacta cagcagacgt tcttcaatcc ggagcttgcc
     2941 ttgcaaaccg gcatcgccgc ctgcgaaacc ggcaacgcct acgagatgga aatgtgcgtg
     3001 gaaaccggca gcggcgcgta tcagaattca cgccaacgac tgatctgggg agaggtccac
     3061 gacggcaacg cgtatttcct cggtggcgcg gccggtcatg cgggcctttt ctcaacggcc
     3121 ggcgacgtat ttcagttggc ccaacaattc atcgctggat ttagccagtt gctcacagcc
     3181 gagacgtgca aactgtttcg ccagaatatg accgagggtt tggacgaggc acgttcgctt
     3241 gcgtggcaat tggcagcgac gaaagactca accgccggaa tcgatctgcc gcccgacagt
     3301 tttggacaca acggattcac aggaacgtcg ctgtggatcg acgccgaaca ccggcgcgtc
     3361 ttcattctgt tgacgaatcg aacgcactcg cgcccgctgc cgttcgcgaa tatcaatgcc
     3421 gtaaggcgtg agtttcatag cctggctata gccgaaatct caaagctgtc cccagcacat
     3481 tgaagtcacc cggcacgctg acgccgtcgt tctgtctgag gtaatagacg tcctgcgtga
     3541 agtgcttatt g
//
LOCUS       DASTEY010000077         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_759712, whole genome shotgun sequence.
ACCESSION   DASTEY010000077 DASTEY010000000
VERSION     DASTEY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759712"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFO72_01335"
     CDS             <1..807
                     /locus_tag="VFO72_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9477955.1"
                     /translation="AVSGLGEKETLQLIYSLALAGVIQRQHWNSVLCDQKTGPPPRPV
                     ALAAAPPEQPVEREQPREIELTDIESFLARVRNAQTHYEVLDVNREVSAESLKKIYYQ
                     LARNYHPDRFRKSHASLVTRLEATFARIKQAYDTLRDDNLRANYDLKLAARKKTEPVA
                     EPIVPLSQPEAVVERSAEPEVRVTESAADRAQRGRMLAGNAKTRRAAEAELLAAIKLD
                     PKNAEYRVMLAELYRDLGLKLRAKGEAERAVAADPNNRKARDLLQALKSV"
     gene            829..1506
                     /locus_tag="VFO72_01340"
     CDS             829..1506
                     /locus_tag="VFO72_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9477956.1"
                     /translation="MQTTDLIDTAAAEAFIQLARTTDKFERYENPHAQRIAVIADEIA
                     QEFHLARHDRGSLYAAALLHDLGEVAMERDYIQATGALSQEERLDLFRHPVIGEREAS
                     RVGADRAAQLLVRWHHEWWNGAGYPDALRREEIPLAARILRVADAYASLTDARPFRRA
                     FTREQARRELMDRVGIEFDPGVVSVLLALEGLPELESFAKRFDAEPAPPPETKSSGWD
                     LFSSFSK"
     gene            1514..>2039
                     /locus_tag="VFO72_01345"
     CDS             1514..>2039
                     /locus_tag="VFO72_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477957.1"
                     /translation="MQFSNGWLGFELGVLRRLKFTSVALPFTGEGEICVHLKNWKVRV
                     AANDPMLWSYTKAQALVENNTEKLNEDDLNTLLDDAYVPHDKLENPALLKWFNETDAW
                     WFDNLRFNAERLESYKRALALTAGMMTGDYVLSFNEQTRNMREPFTLPHVFRRMLDCL
                     PKPQDNGMRNKSTNQ"
BASE COUNT          465 a          560 c          612 g          402 t
ORIGIN      
        1 gcagtcagcg ggctcggcga aaaagaaacg ttgcaactca tctattcgct cgcactcgcc
       61 ggcgtcatcc aacgccaaca ctggaatagc gtgctttgcg atcaaaaaac gggtccgccg
      121 cctcgtcctg tcgcactggc agcggcgccg ccggagcagc ccgtcgagcg cgagcaacct
      181 cgtgagatcg agctaacgga catcgagagt tttctcgcgc gcgtgaggaa cgcgcaaacg
      241 cattacgagg tgctggacgt taatcgtgaa gtctcggctg agagtttgaa gaagatctat
      301 taccagttag cgcggaatta tcatcccgac cggtttcgta aatcacacgc ttctttggtg
      361 actcggctcg aagcaacgtt tgcgcgtatc aagcaggcgt acgacacgct gcgcgacgac
      421 aacctgcggg cgaattacga cttaaaactc gcggcacgaa aaaagactga gccggtggcc
      481 gagcctatag tccccctgtc gcaaccggaa gcagttgtcg aaagatcggc agagcccgag
      541 gtacgggtaa ccgagagcgc ggcagaccga gcgcaacgcg gccggatgct ggccggcaac
      601 gcaaaaaccc ggcgagcggc tgaagcagaa ctgctcgcag cgataaaact cgatcctaaa
      661 aacgcggaat atcgagtgat gctggcggag ttgtatcgag atctcggatt gaagctgcgc
      721 gcaaaaggcg aagcggagcg cgccgtggcc gcggatccaa ataaccgcaa ggctcgtgat
      781 ttgttgcagg cgttgaaatc cgtgtaatct gtgtcgagta tggcggcagt gcaaacaact
      841 gacttgatcg acaccgcagc agcggaagct ttcatccagc tcgcccgcac cacagacaag
      901 tttgagcgat acgagaaccc gcacgcgcaa cgcatcgccg tgatcgccga tgagatcgcg
      961 caggagtttc atttagcgcg gcacgatcgt ggctcgttgt atgccgcggc gttgctgcac
     1021 gatctcggcg aggtcgccat ggagcgcgat tacattcaag ccaccggcgc gttgtcgcaa
     1081 gaggagcggc tcgatctttt tcgtcatcct gttatcggcg aacgcgaggc gtcgcgggtt
     1141 ggagcagatc gcgcggcgca gttgctcgtg cgctggcacc acgaatggtg gaacggcgcc
     1201 ggttatcctg acgcgctgag acgtgaagag attccgttgg cagcgcgaat tctgcgcgtc
     1261 gccgatgcgt atgcgtcact gactgacgcg cggccatttc gtcgtgcatt cacacgagag
     1321 caggcgagac gcgagctgat ggatcgggtg gggattgagt tcgatccggg cgttgtgagt
     1381 gtgttgcttg cactcgaggg acttccggag ttggaatcgt ttgcaaagag gttcgacgca
     1441 gaaccagcgc ctccgccaga gactaagagc agcggttggg atctgttctc gtcgttttcg
     1501 aaatagttta cttatgcagt tttcaaacgg ttggttgggt ttcgaattag gcgtgctccg
     1561 gcgactgaag tttacttcag ttgcactgcc cttcaccgga gaaggagaga tctgcgttca
     1621 cctgaagaat tggaaggtgc gcgtcgctgc caacgatccg atgctctggt cttatacaaa
     1681 agcgcaggcc ctcgtcgaaa acaacaccga aaaactcaac gaagacgatc tcaacacgct
     1741 gctcgacgac gcttacgtgc cgcacgataa gcttgagaat cccgctctgc tcaagtggtt
     1801 caacgagacc gacgcctggt ggttcgataa tctgcgtttc aatgccgagc gtctggaatc
     1861 ttacaagcga gcgctcgcac tcacggccgg tatgatgacc ggcgactacg tgctttcctt
     1921 caacgagcaa acgcgcaaca tgcgcgagcc attcacgctg ccgcacgtat ttcgtcgcat
     1981 gcttgattgt cttcccaagc cgcaggacaa cgggatgcgc aacaagagca cgaaccagg
//
LOCUS       DASTEY010000078         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_726691, whole genome shotgun sequence.
ACCESSION   DASTEY010000078 DASTEY010000000
VERSION     DASTEY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726691"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..677
                     /locus_tag="VFO72_01350"
     CDS             57..677
                     /locus_tag="VFO72_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9477958.1"
                     /translation="MTLKNELSDNELLRLMQAGDEEALAQLYRRRQASVYRFALQMSG
                     SKTIAEDVTQEVFLFLMREGHVFDPARGALSAFLLGVARNHVLRRLRVEQLLSPLSDD
                     TDEESAGLQISTELCPLDDLTRAETIESVRKAVLSLPAKYREVVVLCELQDVSYVETA
                     EILGCAIGTVRSRLHRARALLLTKLRPTESVADADAASVKSARCFA"
     gene            674..1417
                     /locus_tag="VFO72_01355"
     CDS             674..1417
                     /locus_tag="VFO72_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477959.1"
                     /translation="MNCQRFEQVVTDLARSQMMEADLRMEALAHSEKCEHCLARLRDE
                     ETLSRGLQLLAKEKESLAAPDGIELKLREAFRSRQVVVPIAKTSSRSRYWLVAVAAAL
                     LVAMSVVAVWWRSDAPQKTIADTTPPQPAEVRIEQDEPAPKRVESIATNQTPKRQTPK
                     SVRRNPVTRRAPETPLANHEIATDFVALGDMNASSLQDGGQIVRVTLRRSALVRFGIP
                     VNMDRYNENVKADVLIGVDGLARAIRFVQ"
     gene            1430..2470
                     /locus_tag="VFO72_01360"
     CDS             1430..2470
                     /locus_tag="VFO72_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477960.1"
                     /translation="MKRVIGIWLAATLSVSAFASIAFAQQPQLATDEPLIIRQERIFQ
                     GPEGVPPLPPPGADFVFLSSENFGGKVVKGAPYSAEAVTESIQTLADGNRIVNKMTSQ
                     VYRDSEGRTRREQTLKGLGVVGTGEEPLQMIFINDPVAGVSYSLDTRTNTAHKSVPFR
                     FQMTTRSGATAEGQRFEYKIGQGAGATTNVIVAAPLKAPAQARPRVPSGEIEHFKFRT
                     ERAAGYVLRTRTGSAANEVKEQLGRQIIEGVEAEGTRTTVTIPAGEIGNDRPIEVVNE
                     RWYSPELELVVMTRHSDPRSGETNYKLTNINRTEPAKSLFEVPAGFNVKEMQFGARRV
                     APLPRVKKSNPE"
     gene            complement(2514..>3034)
                     /gene="fusA"
                     /locus_tag="VFO72_01365"
     CDS             complement(2514..>3034)
                     /gene="fusA"
                     /locus_tag="VFO72_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9477961.1"
                     /translation="SVPQQFRPAIEKGILEAAQSGAIAGYPLVDFKVQLVDGSYHPVD
                     SDEHSFRAAGRKAFRAAMEKVKPSLLEPIMDVEISAPQEYAGDLMGDLNSRRGRVQGM
                     ESNGKQQVIKAQVPLAEMLNYQSILNSITAARGTFHMQFSHYDPVPGQLAQKIVEQSR
                     AEGRIRGGEEED"
BASE COUNT          694 a          794 c          917 g          629 t
ORIGIN      
        1 cgcacggcgg cgaataccga atgaccaccg agtaaagttt ggggccggct tagcgaatga
       61 ccctgaaaaa cgaactcagc gataacgaat tgctacgcct gatgcaggcc ggcgacgaag
      121 aagcgctggc gcagctttac cggcgcaggc aggcgtcagt ctaccggttt gcgctgcaaa
      181 tgagcggctc gaaaacgatt gcagaggacg tgacgcagga ggtgtttctg tttctcatgc
      241 gtgaggggca cgttttcgat ccggcacgtg gagctctcag tgcgtttttg ctgggagtcg
      301 cacgcaatca cgtgttgcgg cggttgcgtg tcgagcaact gctttcgccg ttgagtgacg
      361 acactgacga agagagcgcg ggtttgcaga tcagcacgga gttgtgtccg cttgacgatt
      421 tgacacgcgc cgaaacgatt gagagtgtgc gtaaagcagt gttgagtttg ccggcgaagt
      481 atcgtgaagt agtggtgctg tgtgaattgc aggacgttag ttacgttgag acagccgaga
      541 ttcttggttg cgccatcggc acggtgcgat cgcgtttgca ccgggcgcgc gcgttgctgc
      601 tgacgaaact ccgcccgact gaaagcgtcg cggacgcaga tgcagcgagt gtaaagtcag
      661 cgaggtgttt cgcatgaact gtcaaagatt tgagcaggtt gttactgatc tggctcgcag
      721 tcagatgatg gaagctgact tgcgaatgga agcgctggcg cacagcgaaa agtgtgagca
      781 ttgtttggcc cggttgcgtg acgaagagac actctcgcgc gggttgcagc tgctcgcgaa
      841 agaaaaggag tcgctggcgg cgcccgacgg aatcgaactt aaattgcgcg aagcgtttcg
      901 cagccggcag gttgttgtcc caatcgctaa aacgtcatca aggtcgcgtt attggttggt
      961 tgctgtggcc gcagccttgt tggtggcaat gagtgtggta gccgtgtggt ggcgttccga
     1021 tgcgccgcaa aaaaccattg ctgacacgac gccgccgcaa cctgctgaag tgcgcatcga
     1081 gcaagatgag cccgcgccga aacgcgttga atctattgct accaaccaaa caccaaaacg
     1141 gcaaacgccg aagtccgttc gtcgcaatcc agtgactcgt cgtgcgccgg aaacgccctt
     1201 ggcgaaccac gagatcgcca ccgactttgt ggcactcggc gacatgaacg cgtcaagtct
     1261 tcaggacggt ggacagatcg ttcgtgttac gttgcgacgt tcggcgctgg tgaggtttgg
     1321 tattcccgta aacatggatc gttataacga gaacgtcaaa gcggatgttt tgatcggggt
     1381 cgatggtctg gcccgtgcca tacgttttgt tcaatgaaag gaaagggaga tgaaaagagt
     1441 aattgggatt tggcttgcgg ctactttgtc agtatcggca ttcgcatcaa tagcatttgc
     1501 ccagcagccg cagcttgcaa cagacgagcc gctgataatc aggcaggagc gcattttcca
     1561 ggggccggaa ggcgtaccac cattgcctcc gccgggagct gactttgttt ttctttcttc
     1621 ggaaaacttt ggcggcaagg tcgtcaaagg cgctccatac tcggccgaag ctgtgacaga
     1681 aagtattcag acactcgcgg acggcaaccg catcgtcaac aagatgacca gtcaggtgta
     1741 tcgcgatagt gaaggtcgta cgcggcgcga acagaccctg aagggtcttg gcgttgtcgg
     1801 cactggcgaa gagcctttgc agatgatctt tatcaacgat ccggtcgccg gcgtgagtta
     1861 ttcgctcgac acgcgcacga acactgcgca caagagcgta ccgttcagat ttcaaatgac
     1921 cacaagatcg ggcgcaactg cggaaggcca gcggtttgaa tacaagatcg gccagggcgc
     1981 cggcgccaca acgaacgtga tcgtggcggc gccactcaag gcaccggcgc aagcaagacc
     2041 gcgagtgccg tctggtgaga tagaacattt caagtttcga actgaacgcg ccgcaggtta
     2101 cgtcctgagg accaggacag gctcggctgc caacgaagtg aaagaacaac tgggcagaca
     2161 gataattgaa ggcgtcgagg cggaaggcac gcgtacgacc gtgacgattc cggcgggaga
     2221 gattggcaac gaccggccca tcgaagtcgt caatgaacgc tggtactcac cggaactcga
     2281 actagtggtg atgacgcgcc acagcgatcc gcgttccggc gagaccaatt acaaactgac
     2341 gaacatcaac cgcacggaac cagcgaagtc tctattcgaa gtgcctgctg gtttcaatgt
     2401 gaaggaaatg cagttcggcg cgcgtcgcgt cgctccattg ccacgtgtaa agaagtcaaa
     2461 ccccgagtaa aacccagtcc acagattaca cagaaaatct gtgtaatctg tggttagtct
     2521 tcctcttctc cgccgcggat gcgtccttct gcacgcgact gctccacgat cttttgggcc
     2581 aactggccgg gtacaggatc gtagtgcgaa aactgcatgt ggaaagtgcc gcgcgcggcg
     2641 gtgattgagt taagaatcga ttggtagttg agcatctcgg cgagcggcac ctgcgctttg
     2701 atcacctgct gtttgccatt ggattccatt ccctgaacac gtcctcgccg cgaattcaga
     2761 tcgcccatga gatcaccggc atactcctgc ggcgccgaga tctcgacgtc cataataggc
     2821 tccagcagcg acggcttgac cttctccatc gccgcgcgga atgccttgcg acccgcagca
     2881 cggaacgaat gttcgtcgga gtcgaccgga tgatacgagc cgtcaaccaa ctgcaccttg
     2941 aagtccacca gcgggtaacc agcgatagcg cctgattgtg ccgcttcgag aattcctttt
     3001 tcgatcgccg ggcggaactg ttgcggtacc gaac
//
LOCUS       DASTEY010000079         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1336425, whole genome shotgun sequence.
ACCESSION   DASTEY010000079 DASTEY010000000
VERSION     DASTEY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336425"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFO72_01370"
     CDS             <1..261
                     /locus_tag="VFO72_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477962.1"
                     /translation="YSIAAPGTSQHIAMLAFDVNEFENPRVREIMAKHGWFQTVLSDL
                     PHFTFLGRKEKDLPKLGLRSVEVNGQVFWIPNVSAAHPGLDK"
     gene            431..2053
                     /locus_tag="VFO72_01375"
     CDS             431..2053
                     /locus_tag="VFO72_01375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9477963.1"
                     /translation="MDVPQFIAGRFRIEREIGTGGMGTVYLATHLDLERPVAVKIIKR
                     EFAADADVAERFLREARTMAKLRHPHAAMIFDAGNLPDGRHFIVMEFIEGETLSQVLA
                     REGRFAASRAVEIATQICDVLEEAHRLGIIHRDLKPSNILLGPRGVCVLDFGVAKVLA
                     SSAESTATCASTGSGQLIGTPRYMSPEQCLGQRVGARSDLYSLGVLLYEMLAGRPPFV
                     DPLQSALLIKQATAAAPPLPKLRPDIGRPLALAVHTLLAKRPDDRPRTAAIAKSLLER
                     SITQPERTLPDMEPLSAMVAAADAGRGILFRFGAPAFLVTTFGALLAWGYTGQSAPEV
                     RLPKTALPLAASQMQMKPATWPKSEPAPLQNAAEMRILPQPVEQLSLAQARKIAAKFV
                     RGSLGAMEIVETDAGSAIVAINNQRKAGTSSFVLLQKRGGRFGVSAQGRLDARGFSHA
                     SWSAELVDADEDGYQELLFSGKDSAESRNLRRLILFVPNNRRTYSMQMTGETTSRGTP
                     KIQWLSNATGTDAAAYRTTLRRKARSIVSKTR"
     gene            2167..>2611
                     /locus_tag="VFO72_01380"
     CDS             2167..>2611
                     /locus_tag="VFO72_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET9477964.1"
                     /translation="MKRTFLVSLLLFCLLTPSTLLVGAVPQRRTAKPNASTPAPTTPA
                     AAPARLPEAKIFSHVLPNGLEVLILEDHSIPLVTIELAVKNGSYTEPPELNGLSHLYE
                     HMFFKQNRAIANKELYLHAIGQLGIAYNGTTREEVVDYYFTTTSPN"
BASE COUNT          588 a          745 c          716 g          562 t
ORIGIN      
        1 tattccatcg ctgcgccggg cacttctcaa cacattgcga tgctcgcgtt tgacgtgaac
       61 gagttcgaaa acccgcgcgt gcgtgagatc atggcaaagc acggttggtt tcagacggtc
      121 ctgagtgacc tgccgcattt cacttttctc ggccggaaag agaaagacct tcctaaactt
      181 ggtttgcgca gcgtcgaagt aaatgggcag gtgttttgga taccgaacgt atcggccgca
      241 catccgggac ttgataagta ggacagacat tcctgtccgt ctccgtgaca ggcaagaatg
      301 cctctccgtg ccagacaaga atgtctgtcc tacaaagtgt gctaaactag gccgtctctt
      361 cccccagccc attcaaaatt tgttgtcagt cgttgctcct ggcggccttt accccgaaag
      421 aaggtctgat atggatgtcc cccaatttat cgccggcagg ttcaggatcg agcgcgaaat
      481 cgggaccggc ggcatgggca ctgtctatct agcgactcat ctggatctcg aacgacctgt
      541 agcagtcaaa atcatcaagc gcgaattcgc cgccgatgcg gacgtggcgg aaagattttt
      601 gcgcgaagcg cgcacgatgg cgaagttgcg ccatccacac gccgcgatga tcttcgacgc
      661 gggcaatttg cctgacggtc gtcacttcat cgtaatggag tttatcgaag gcgaaactct
      721 gtcgcaggtc cttgctcgcg aaggaaggtt tgctgccagc cgcgccgtcg agatcgccac
      781 acagatctgc gacgttctgg aagaagcaca tcgtctcggc atcattcatc gcgacttgaa
      841 accgtcgaac attctgctcg gcccacgcgg tgtttgtgtg ctcgacttcg gcgtagccaa
      901 agtgctcgcc tcatcagcgg agtccacggc aacgtgcgcc agtactggtt cgggccagtt
      961 gattggcacg ccgcggtaca tgtcgcccga acaatgtttg ggccagcgtg tcggcgcgcg
     1021 cagcgatctc tacagtcttg gggtgttgtt gtatgaaatg ctcgccggtc gtccgccgtt
     1081 cgtcgatccg ctgcaatcgg ccctgctgat caaacaagcc acagcggccg caccaccgtt
     1141 gccgaaactg cgaccggaca tcgggcgtcc gctcgcgttg gcagttcaca ctctgcttgc
     1201 gaagcgtcct gacgatcggc cgcgcacggc agctatcgca aagtcgctcc tcgaacgcag
     1261 catcacgcaa cccgaacgca ctttgccgga catggaacca ctttccgcga tggtcgccgc
     1321 ggcagatgcg ggacgcggca tactgtttcg ctttggcgct cctgcgtttc tcgtaacgac
     1381 atttggcgcg ttactggcgt ggggttacac aggccaaagt gcacccgaag tgcgtttacc
     1441 aaaaactgca ttgccactcg ccgcatcgca gatgcagatg aagcctgcaa cgtggcccaa
     1501 aagtgaaccg gctccgctgc aaaatgcagc agagatgcga atacttccgc aaccagtcga
     1561 acagttgtca ctcgcacagg cccgaaagat agcggcgaag tttgttcgcg gttcactcgg
     1621 cgcgatggag atcgtcgaga ctgatgccgg ttcggcgatc gtcgcaatca acaatcaacg
     1681 aaaagcgggc acgagcagtt ttgtcctgct tcagaaacgt ggcggcaggt ttggtgtctc
     1741 tgcgcaggga cggctcgacg ctcggggatt cagccatgcg agctggagtg ctgaactggt
     1801 tgatgcggat gaagacggtt atcaggagtt gttgttcagc ggtaaagatt cggcagagag
     1861 ccggaattta agaagattga ttctgttcgt acccaataac agacgcactt actcgatgca
     1921 gatgacgggc gagaccacct cccgcggcac acccaaaatt cagtggctgt cgaacgccac
     1981 gggaactgac gccgctgcct accgaacgac tctgcgaaga aaagcccgct ccattgtctc
     2041 caaaacgcgc tgaagaatat acgaaccacg gaacacacag ataaaagaca aattctgtgt
     2101 attccgtgta atctgtggtt cgctgagccg catctattgg gttaacttag tagcatggag
     2161 tcatacgtga aacgaacctt cctggtatcg ctgcttcttt tttgtttatt gacaccttcg
     2221 acgctcctgg tcggagcggt cccgcaacgc agaacggcaa aaccgaatgc aagtacgccg
     2281 gcgccgacca cgcctgcggc agcgcccgcg cgattgcccg aggccaaaat attcagccac
     2341 gtgctgccga acggtttgga ggtcctcatt ctcgaggacc actccattcc gctcgtcaca
     2401 atcgagttgg cagttaaaaa cggctcctac accgaaccgc cggaacttaa cgggctgtcg
     2461 catctttacg agcacatgtt cttcaaacag aaccgtgcga ttgctaataa ggagttgtac
     2521 cttcacgcca ttgggcagtt gggtatcgct tacaacggca cgacgcgcga agaagttgtc
     2581 gattattact tcaccacgac cagtccaaac c
//
LOCUS       DASTEY010000080         5531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_891594, whole genome shotgun sequence.
ACCESSION   DASTEY010000080 DASTEY010000000
VERSION     DASTEY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891594"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /locus_tag="VFO72_01385"
     CDS             <1..1795
                     /locus_tag="VFO72_01385"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9477965.1"
                     /translation="RAIKVTYEVLPHVVHADAAIRADAPKVVAQENNLQEKNKNGDPA
                     KVEAAFATADAFVEAEYISPRVHHACLETHGMVVDYRGGDTATIYASTQGTFTIPADA
                     ARELGLEQSNVVSSVEHMGGGFGSKFGIGTEGMFACRLSKQAKAPVKLMMTRYDEFVM
                     AGNRSGSWQKLKAGVKNDGTIVALQARQYRLGGIGQGSQAGQPYIYRMGETYREIYAL
                     HTNEDSSVAMRAPGHPQASFAVESLVDELAYKIKMDPVAFRKKNLRDEVYHRQLDRGA
                     KEIGWSRRNPVAGGNPGPLKRGLGCGVGTWGGGGNNQCKVALTISRDGSVLVAVGTQD
                     LGTGTRTYTRAIVAEELGLGMNDIREGIGNSRLGSANPSGGSTTAASLAPSVKDASIK
                     ARLMMAERVAPLLGNAKADEVVFAAGKVSAKGQSLTWKQACAALPAAGITAHGEWRSD
                     LQSRGIHGVCFAEVEVDVETGHIQPIKMVHVQDCGLPLNRLALESQINGGMIQSLGQA
                     LWEGRVIDAHLGLQLNPGFGDYKLPGSLEMPELVPIIDDDDKREAVIGVAEGCNIPAL
                     GALANAVFNATGIRIRELPITPDKILMGTQS"
     gene            1792..2796
                     /locus_tag="VFO72_01390"
     CDS             1792..2796
                     /locus_tag="VFO72_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9477966.1"
                     /translation="MNSFEWANPTTVNEAVKLLRVQPAGGDIDEAPRPLAGGQDLLTT
                     MKDYTSRPTRLVNLKHIPGLDRITLNARGLTIGALVTLTQLEEHAGVRKSFPGLAEAA
                     ESIATPQIRNLGTVGGNLCQRPRCWYFRLEEVICLKKGGDECYAATGENKYNAIIAGG
                     PSFIVHPSDLAPMLLALGARVTVMGATGRRVIPLDKFFTLPTEGNIRRENVLENDDII
                     TEIFVPASPLAARSTYLKFKERDSLDFALAAVATALRLGPNGAVRDVRIVLGGVAPIP
                     WRVPAAEKFLIGKRLQADVLAEAARMALRDAEPLEKNAYKVPLAQTLVRRALAKVGNV
                     "
     gene            2814..3077
                     /locus_tag="VFO72_01395"
     CDS             2814..3077
                     /locus_tag="VFO72_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477967.1"
                     /translation="MADERIETTAETLSAPEPLQSAFCGSLRSKKFFLLDRLAASADD
                     YLDAANHVWCCETQEVIGPDNGRVAPDTCGPGRACYSSAVKGL"
     gene            3112..3768
                     /locus_tag="VFO72_01400"
     CDS             3112..3768
                     /locus_tag="VFO72_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET9477968.1"
                     /translation="MRRVLFVAAVMVVAVCALVFSSQAESVSLRGEYVEVRTASVFAG
                     ACHYNGEVVTTGRDAVMAWNVTSGKWRGVELAGVRALAIITADANLSDDKAARQSEML
                     IDAQASNAQSQAMIDALKVKYAAALGNVVTVRNTPISFERKGRSYAVAADGAFIDIEA
                     MPNDLCCKMPNLVWYSPLVRLENRKVGYTVRAGYSGKTISTPWSRSGENSAFYGSFSL
                     "
     gene            complement(3877..4335)
                     /locus_tag="VFO72_01405"
     CDS             complement(3877..4335)
                     /locus_tag="VFO72_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477969.1"
                     /translation="MRNFYRRFSLESLCYARRDVRVARLILTKFGVALPQKSLSPDRA
                     LARCKGTVFRRIASETVKTVSQKPRARAYLVKKVCKHFPESPGYVLGVTSKRVLYSLQ
                     LDGRDRKLINQLAADVSYAGYTFIIPLEHSHKPLIKIFKHIEGAEIYRAV"
     gene            4418..>5531
                     /locus_tag="VFO72_01410"
     CDS             4418..>5531
                     /locus_tag="VFO72_01410"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9477970.1"
                     /translation="MPQDRISIFDTTLRDGEQSPGCSMDLREKVRMAEKLEELGVDII
                     EAGFPIASEGDFAAVRAVARQCREVAIAALCRTAERDVARAAEALARAAHPRIHTFCA
                     TSDIHLEHKLKKTRAEVIEMTRAAVRQARSYAEDVEFSAEDATRSDVDYLCEVLDVAV
                     DAGASVLNVPDTVGYTTPPEMSRLVQQVRERVVRDRNVIVSVHCHNDLGLAVANSLAA
                     IEAGARQVECTINGIGERAGNASLEEIVMALQVRADRLPFQTGIATRELYPASQLLSS
                     IVGFEVQPNKAIVGRNAFAHEAGIHQHGVINNPLCYEIMTPESVGVPANSLVLGKHSG
                     RHALSLRYVELGYHVTPAELDVAYRKFTDLADKKKRI"
BASE COUNT         1204 a         1537 c         1684 g         1106 t
ORIGIN      
        1 gcgtgcgatc aaagtcacgt acgaagtttt gcctcacgtc gtccacgctg acgccgcgat
       61 tcgcgcggat gcgcccaaag tggtcgccca ggagaacaac cttcaggaaa agaacaagaa
      121 cggcgatcca gcgaaagtcg aagcggcttt tgccactgcc gacgcgttcg tcgaagccga
      181 atacatctcg ccgcgcgttc accacgcctg tctcgaaacg catggcatgg ttgtcgatta
      241 tcgcggcggc gacacggcca cgatttacgc ttcgacacag ggaacattca cgattcccgc
      301 cgacgcagca agagaattgg gactcgagca gagcaacgtt gtctcgagtg tcgagcacat
      361 gggcggcggc tttggctcaa agtttggcat cggcaccgag ggaatgtttg cctgccggct
      421 ttcgaaacaa gcgaaagcgc cggtgaagtt gatgatgacg cgttacgacg agttcgtgat
      481 ggcaggcaat cgctcgggct catggcaaaa actgaaagca ggcgtgaaga acgacggcac
      541 gatcgtcgca ctgcaagcgc ggcagtatcg tctcggcggc atcggccagg gctcgcaagc
      601 cggtcagccc tacatctatc gcatgggcga gacctatcgc gagatctacg ccttgcatac
      661 gaatgaagac tcgtcagtcg cgatgcgcgc gcccggtcat ccgcaagctt cgtttgcggt
      721 tgagtcgctc gtcgacgaac tcgcctacaa gatcaagatg gatccggtcg cgtttcgtaa
      781 gaaaaacctg cgcgacgagg tctatcaccg ccagttggac cgcggcgcca aagagattgg
      841 ctggagcaga agaaaccctg ttgccggcgg caatcctggt ccattgaaac gcggcctggg
      901 ttgcggcgtc ggcacgtggg gcggcggcgg aaataatcag tgcaaagttg ctttgacaat
      961 ctcgcgtgac ggttcggttt tagtcgcggt cggcacacag gatctcggca ctggaacacg
     1021 aacgtacacg cgcgcgatcg tggctgagga actcggtctc ggcatgaacg acatcaggga
     1081 ggggatcgga aactcgagac tcggttcggc aaacccgtcc ggtggctcaa ccaccgcggc
     1141 atcgctcgcg ccgtcagtga aagacgcgtc gatcaaagcg cgcctgatga tggctgagcg
     1201 cgtcgcgccg ttgctcggca atgcgaaagc ggatgaagtt gtgtttgccg cgggaaaggt
     1261 cagtgcgaaa ggtcagtcac tcacgtggaa acaggcgtgc gccgcgcttc cggccgcggg
     1321 tatcaccgcg cacggcgagt ggcgttcgga cttgcaatcg cgcggcattc acggcgtctg
     1381 ttttgcggaa gtcgaagtgg acgtcgaaac gggtcacatc cagccgatta agatggtcca
     1441 tgtccaggac tgcgggctgc cattgaaccg tctggcactg gaaagccaga tcaacggcgg
     1501 catgattcag tcgctgggcc aggctttgtg ggaaggccgc gtgatagacg cgcatttggg
     1561 cctgcagctc aatcccggct ttggcgacta caagctgccc ggcagtctcg agatgccgga
     1621 actcgttccg atcatcgacg acgacgataa acgcgaggca gtgatcggcg tcgcggaggg
     1681 ctgcaacatt cccgcgctcg gcgcgttggc gaatgcggtg ttcaatgcga cggggattcg
     1741 tatacgcgaa ctgccgatca cgccggacaa aattttgatg gggacacaat catgaattcg
     1801 tttgaatggg ccaatccgac gacggttaac gaggccgtga agctgttgcg tgtgcagccg
     1861 gcaggcggcg acatcgatga agctccgcgg ccgcttgcgg gcgggcagga tctgttgacc
     1921 acgatgaagg actacacgtc gcggcccaca cgcctcgtga atctcaagca tattcctgga
     1981 ctcgaccgca tcacgctcaa cgcgcgcgga ctcacaatcg gcgcgctcgt gaccttgacg
     2041 caactcgaag aacatgccgg agtgcgcaag agttttccgg gtctcgccga agccgcggaa
     2101 tccatcgcca cgccgcagat tcgcaacctc ggcacggtcg gcggcaacct ctgccagcgc
     2161 ccgcgctgct ggtacttccg tctcgaagag gtgatctgtt tgaaaaaagg cggagatgaa
     2221 tgctacgcgg ctaccggcga gaacaagtac aacgcgatca tcgctggtgg tccgtcgttc
     2281 atcgtgcatc cgtctgacct ggcgccgatg ttgctcgcgc ttggtgcgcg tgtcactgtt
     2341 atgggcgcga caggacggcg agtaattccg ctcgacaagt ttttcacgtt gccgaccgag
     2401 ggcaacattc gtcgcgagaa cgttttggaa aacgacgaca tcattacaga gatctttgtg
     2461 cccgcctctc cgctcgccgc gcgttcgacg tacttgaagt tcaaagagcg cgattcactc
     2521 gacttcgcgc ttgctgccgt ggccaccgcc ctgcggcttg gtcccaacgg tgcggtgcgc
     2581 gacgttcgca tcgtgctcgg tggcgtagca ccaataccgt ggcgcgtgcc ggcggcggaa
     2641 aagtttctca ttgggaaacg gctgcaagct gacgtgctgg ctgaagccgc caggatggcg
     2701 ctcagggacg cggagccgct ggaaaagaac gcctacaaag ttccgctggc gcagacgctt
     2761 gtgcggcgcg cgctggcgaa ggttggaaat gtttgacaaa tggagtcgtg atcatggctg
     2821 acgaaagaat tgaaacaacg gccgaaacac tttctgcacc tgagcctctt cagtcggctt
     2881 tctgtggctc gttgcgttcg aagaagttct ttttactgga tcggttagcg gccagcgcgg
     2941 acgattatct cgatgcggca aatcacgtct ggtgttgcga aacgcaggaa gtgattgggc
     3001 ctgacaacgg gcgtgtcgca cccgacacgt gcgggccggg ccgcgcatgc tacagttccg
     3061 cggtcaaagg gttataatcc gtcttatctg aggttcggag gtttttgagt aatgcgaaga
     3121 gtattgtttg tagcggcggt gatggtggtg gcggtttgcg cgctcgtttt cagttcgcag
     3181 gcggagagtg tcagtttgcg tggtgagtat gttgaagtgc gcacggcaag cgtgtttgcc
     3241 ggcgcgtgtc actacaacgg cgaagtcgtc acgaccggtc gtgatgcggt gatggcgtgg
     3301 aatgtgacct ccggaaaatg gcggggcgtc gaacttgcgg gcgtccgtgc gctggcgatt
     3361 attacggccg acgcgaattt gtccgacgac aaagcggcgc gacaatccga gatgctcatc
     3421 gacgcgcaag cctcaaacgc acaatcacaa gcaatgatcg acgcgctgaa agtaaagtac
     3481 gctgccgcgc tcggcaacgt tgtgacggtt cgcaacacgc cgatcagttt tgaacggaaa
     3541 ggccgcagtt acgctgtcgc agcggacggc gcgtttatcg acatcgaagc tatgcccaac
     3601 gatctttgct gcaagatgcc aaacctcgtc tggtactcgc cgctggtcag attagaaaac
     3661 aggaaggtcg gctacacagt cagggccggc tattccggca aaacgatcag cacgccatgg
     3721 tctcgctccg gcgagaacag cgccttctac ggcagcttct cgttgtaacg acaccagccg
     3781 caacacgaaa ctatctcatt ctcacccggc ttcagccggg tgtccgcgcg tcttggcagg
     3841 aaaaccgttt caacggtttt ccgcagcgcg acggatctaa acggcccgat aaatctccgc
     3901 gccttcgatg tgcttgaaga ttttgatcag cggtttgtgg ctgtgttcca gcgggatgat
     3961 gaaggtgtag ccggcgtaac tcacgtccgc agctaactga tttatcaact tccggtcgcg
     4021 cccatcgagc tgcaaactgt ataagacacg cttcgaggtc acacccagaa cataacccgg
     4081 agactcggga aaatgtttgc aaactttctt caccagatac gccctggctc gcggtttttg
     4141 agaaaccgtt ttaacggttt cgctggcgat tctacgaaaa accgtgccct tgcagcgggc
     4201 taaagcccgg tcaggagaaa gggacttttg gggcaaagca actccgaatt tggtgagaat
     4261 caggcgggca accctaacgt cgcgcctagc ataacaaaga gactcaagcg aaaatctacg
     4321 ataaaaattt ctcatctttc cataaagatt atagctttga ctcttggacc aactgtcctt
     4381 agcatcctca ttcgcttgcc cacaggagga ctccccaatg ccacaggaca gaatcagcat
     4441 cttcgacacc accttacgcg acggcgaaca gtcgcccggg tgttcgatgg acctgcgaga
     4501 aaaagtcagg atggcggaaa agctcgagga actcggcgtc gacatcatcg aagcagggtt
     4561 tcccatcgcg tccgaaggcg acttcgccgc ggttcgcgcg gtggccaggc agtgccgcga
     4621 agtcgcaatc gccgcactct gtcgcaccgc tgaacgggat gttgcacgtg ctgctgaagc
     4681 gctggcgcgg gcggcccatc cgcgaatcca caccttctgc gcgacgtctg acattcatct
     4741 cgaacacaaa ctcaagaaga ctcgcgccga ggtgatcgaa atgactcgcg ccgccgttcg
     4801 ccaggcgcgc agttacgctg aagatgtaga gttttcagcc gaagacgcaa cccgcagcga
     4861 cgtcgactat ttgtgcgaag tgctcgatgt ggctgtcgat gccggggctt ccgtgctgaa
     4921 cgtaccggac acggtcggct atacgacgcc tccggagatg tcgcgtttgg tccagcaggt
     4981 ccgcgagcgg gtcgtcagag acaggaacgt gattgtcagt gttcactgcc acaacgatct
     5041 cggcctcgca gtggcaaaca gtctcgcggc aattgaagcc ggggcgcgac aagttgaatg
     5101 cacgatcaac ggaatcggcg agcgcgcagg caacgcctcg ctggaggaga tcgtaatggc
     5161 gctacaggtt cgcgccgatc gtttgccgtt tcaaaccggc atcgcaacgc gggaactcta
     5221 tcctgcgagc cagttgctct cgagtatcgt cgggttcgaa gtgcaaccga acaaagcaat
     5281 cgttggtcgc aatgcgtttg cgcacgaggc cggcatacat caacacggcg tcattaacaa
     5341 tccgctctgt tacgagatca tgacgcctga atcggttggc gttcctgcta actcgctggt
     5401 gctcgggaag cattccggac ggcatgcgtt gtcgttgcgg tacgtagagc tgggatatca
     5461 cgttactccg gcggagcttg atgtggcgta cagaaagttc acggatttag cagacaagaa
     5521 aaaacgtatt t
//
LOCUS       DASTEY010000081         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_479708, whole genome shotgun sequence.
ACCESSION   DASTEY010000081 DASTEY010000000
VERSION     DASTEY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479708"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VFO72_01415"
     CDS             <1..1611
                     /locus_tag="VFO72_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9477971.1"
                     /translation="LVTITWACLTVAALCLSTTAATQTSPQKTEAETSQQERPLTPAE
                     RRGKAIYFRGETSGKGEVTAIIGDVDVPASTVTCAGCHGRRGEGKTEGGVTAGNLTWS
                     NLIKPYGHTHSSGRKHGPFTESSFAMALVRGVDPAGNNLVVAMPRYRLSIEDMNDLIA
                     YVKRLEFDRDPGLTAETIDIGVPLPTTAALAETVQAIRLVLTAYFDELNAQGGIYNRK
                     IKLHFTESSSAEMSSSLRASMTKNPVFAFVGGLSAGVDTELSALAREQEVPFIGPATL
                     LPLVEQPPNRYVFYLLPGVSEQAAALVNFADQQLGLKKQRSAVLFLTNELQTAAAEAA
                     LAYAKQVGWNDVERIPHPGGSFDAKGLAQRLKAEGTAAIFIFGTGNEVESFVRAASAL
                     EWTPHVLSLGVLASRDLAANLPLSFTKKIFLSFPTVPGDVTAAGMSEYRALADKYKLQ
                     PRHTAAQLAALAAAKTFVEALKRAGADLSREQLITALESLYDYETGLTPKLIFGPNRR
                     IGAAGAYIVTINAETKQFVSVSGWVPAN"
     gene            1920..2261
                     /locus_tag="VFO72_01420"
     CDS             1920..2261
                     /locus_tag="VFO72_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477972.1"
                     /translation="MDEYLDGLFWISVFGLAFIIGGTVVLKKAGLRDLVVISYLILST
                     VIFLVNFGFSLWGALSIMRSSKDGKLTMQPGHDTRELSPPSFEPLPISVTSVTENTTR
                     SFEPVEQKRPR"
     gene            complement(2289..>2752)
                     /locus_tag="VFO72_01425"
     CDS             complement(2289..>2752)
                     /locus_tag="VFO72_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477973.1"
                     /translation="QETELSAYDDPERYPNIAVIQLTDTPEKVLKYTEAMKLQRGLVD
                     LPALVLSWLGFVWCVGKKGNPLLDGEGLPSAVFAESLFGLAGIELTPGLASASTCPEA
                     IWQAAKWWASFYEDTRTLATPNHAQVIVPKGCFVLRQPNPAIRKREQPAQK"
BASE COUNT          638 a          798 c          749 g          567 t
ORIGIN      
        1 ctcgtcacca ttacttgggc gtgcttaaca gtagcagcgc tgtgtctctc gacaacagcc
       61 gcgactcaaa catcgccaca gaaaaccgaa gccgaaacgt cgcagcagga gcgtccgtta
      121 acgccggctg agcggcgtgg caaagccatc tattttcgag gtgagacttc aggcaaggga
      181 gaagtcaccg ccataatcgg cgacgtggac gtgcctgcga gtacggtcac ttgcgccggc
      241 tgccacggcc ggcgaggcga aggcaaaacg gaaggtggcg ttactgcggg aaatctgacg
      301 tggtctaatc tcataaaacc atacggccac acgcactcct ctggccgcaa acatggtccg
      361 ttcaccgaat cgtctttcgc gatggcattg gtccgcggtg tagatcctgc gggtaacaat
      421 ctcgttgttg ctatgccgcg ttaccgctta tcgatcgagg acatgaacga cctcattgcc
      481 tacgtcaagc gactcgagtt tgatcgagat cccggtttga ctgccgaaac catagacatc
      541 ggcgtgccgc ttccaaccac cgccgctctg gccgagaccg tccaggctat acgactagtc
      601 ttaactgcct acttcgacga gctaaacgca caaggtggaa tctacaaccg aaaaatcaaa
      661 ctccatttca ctgaatccag cagcgccgaa atgtcgagca gcctgcgcgc gtccatgact
      721 aagaacccgg tgtttgcatt cgtaggcggt ttgagtgccg gtgtcgatac agaactatca
      781 gctcttgcac gcgagcaaga agttccgttc atcggaccgg cgacgctgct gccactagtc
      841 gaacaaccgc cgaaccggta cgtcttctac ctgttgccgg gcgttagtga acaagccgcg
      901 gcgctcgtga attttgcaga ccagcagtta ggtttgaaga agcagcgttc cgccgtgctc
      961 tttctgacca acgaattgca gacagcggcg gcagaagctg cgctggcgta cgcgaagcaa
     1021 gtgggatgga atgacgtcga aaggattcct caccccggcg gaagctttga cgcaaagggg
     1081 ctggcacaac gactaaaagc ggaaggcacg gcggcaatat tcatcttcgg aaccggtaat
     1141 gaagttgaaa gttttgtgcg tgccgcaagc gcgctcgaat ggactccgca cgttttgtcg
     1201 ctgggcgtac tagcgtcaag agatctcgcc gccaatcttc ctttgtcgtt cacgaaaaag
     1261 attttccttt cgtttccgac tgtgcccgga gatgtgacag ccgcgggcat gagcgagtat
     1321 cgtgctctcg ctgacaagta caaactgcaa ccgcgtcaca cggcagcaca actcgcagca
     1381 ttggcggcgg cgaagacgtt cgtcgaggct ctgaagcgtg cgggcgcaga cttgagtcgc
     1441 gaacaactga tcaccgcgct cgaaagtctt tatgattacg aaacaggact gacgccaaaa
     1501 ctgatctttg gccccaaccg ccgcatcggg gccgctggcg cttacatagt cacgatcaac
     1561 gcagaaacta agcagtttgt gtcggtaagt ggttgggtac cggcgaattg atgacttgtt
     1621 gattcagttg cttaggtttg ccctaaactt caacgggcgg catcacccgg gccttcggcg
     1681 ctaggcacaa cggccggcgg tcaccgaccc ttcggcgcta ggcgcaacgg ccgacgtcac
     1741 ccgcgccttc ggcgctgggc acaacgacga tcgtgacgta ctatgcgggg gacacgccaa
     1801 tgtactgcca gaactgcgga agcgctcttg ctcacaaaac gaaatactgc aaccgttgtg
     1861 gcactcaact cgcgccggca actaatacgc ccgtcgaaaa gaccccggcc gaaaagcgtt
     1921 tggatgagta tttagacggt ctgttctgga tcagtgtatt cggcctggcc tttatcatcg
     1981 gcggcacggt ggtcctgaag aaggccggac ttcgcgatct ggtagtaatc agctacctga
     2041 tactcagcac cgtaatcttc ctggtgaatt tcggcttcag cctctgggga gcactcagca
     2101 ttatgcgcag ttcgaaggac ggcaaactga cgatgcaacc tggacacgac acacgcgaac
     2161 tctcgccgcc atcatttgaa ccgctaccga tatcggtgac aagtgtcaca gaaaacacga
     2221 cgcggtcctt cgagcctgtc gaacagaagc gtccgcggta gcgccgcttc caacacctat
     2281 cagtaaagtc atttctgtgc cggctgctcg cgctttcgta tcgccggatt tggttgccga
     2341 agaacgaagc aacctttcgg tacgatcacc tgcgcgtgat ttggagtcgc gagcgtacgc
     2401 gtgtcttcat agaacgacgc ccaccatttt gccgcctgcc agatcgcctc ggggcacgtc
     2461 gaggctgacg ccaaaccagg cgtgagctct atgcccgcga gtccaaacaa gctttcagca
     2521 aacacagcac taggcaaacc ctccccgtcc aacaaaggat tacctttctt accaacacac
     2581 caaacaaagc cgagccacga cagcactagc gccggcagat cgacaagccc tcgctgcaac
     2641 ttcatcgcct ctgtgtattt gagcactttc tcgggagtgt ctgtcagttg gataaccgcg
     2701 atattggggt aacgttccgg atcgtcgtaa gccgatagtt cggtttcctg ca
//
LOCUS       DASTEY010000082         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1303555, whole genome shotgun sequence.
ACCESSION   DASTEY010000082 DASTEY010000000
VERSION     DASTEY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303555"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1429
                     /locus_tag="VFO72_01430"
     CDS             110..1429
                     /locus_tag="VFO72_01430"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9477974.1"
                     /translation="MRPQDVIRKKRDGLSLTREEIEFFVAGVTSGRIADYQVSALLMA
                     IYLNGMNEAEQQALTEAMLKSGNILDFSDIPKPKADKHSTGGVGDKTSIIIAPLVAAC
                     GVCVPMISGRGLGHTGGTLDKLESIPGYRVALSASEFKTVLEKVGYAMAGQTAELAPA
                     DKKMYALRDATATVEAIPLIVASIISKKGAAGLDAMIIDVKVGNGAFMRQEDRARALA
                     HALVSTGNSCGIKTRALLTDMNQPLGGAVGNSLEVEECINILRGEANEAAAPVLELSL
                     ELSAHMLVLANVDKTIEAARQRLQGVLDSGKALECLQSNIEAQGGDPRVCDAPGDFLP
                     LVSETVKVESPRSGFITKVDTTEIGHAIAAIGGGRVRIEDTVDPTVGFTSDLKLGDQI
                     RAGDAIGTVYCADAAAADEAARRIQAAYHVSDEAPAELKLVKEIINK"
     gene            1426..2457
                     /locus_tag="VFO72_01435"
     CDS             1426..2457
                     /locus_tag="VFO72_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9477975.1"
                     /translation="MKFKLVLVVLFIALTSLLLSGCGGSSAAEDPTKIKVGIVFDIGG
                     KDDRSFNAAAWEGVQRAARDLPIVLRDIEPGTPNAIEPAMRAFAERNFDLIIGVGFAQ
                     APIMELVAKDYPHIQFAIIDGVSKLPNVASLVFKEHEGSYLVGILAAKSSRTGTIGFL
                     GGMDIGLIHRFAKGYEEGAKSVNPNIRVIENYVGVTDSAWNNPGKGKELSLAQIGKGA
                     DVIFTAAGNSGLGAFDAVEQQGKQNGRATHFVIGVDSNQNMVKPGFVLTSMVKRVDNV
                     VYDIVKEVVDHKFSPGVHVFGLDRDGVGYAMDEYNKDLVTPEATQQAEEAKKKIIAGE
                     IKVTDAIGS"
BASE COUNT          563 a          644 c          751 g          504 t
ORIGIN      
        1 tgtgtgagcg tgaaagcgaa gacgggcgag ggcgtcgatg cggtggggca ggggctcgct
       61 gtgaccgctc aggccgctgt tcttatggag agcaactaag gaaagctgct tgagaccaca
      121 agacgtcatc agaaaaaagc gagacgggct gagcctgacg cgtgaagaga tcgagttctt
      181 cgtcgcgggt gtaaccagcg gtcgcatcgc cgattatcag gtgagcgcgc tgctcatggc
      241 catttacctc aatggcatga atgaagccga gcagcaggct ttgaccgaag ccatgctcaa
      301 gtccggcaac attctcgact tctctgacat tcccaaacca aaagcagaca aacactcgac
      361 cggaggcgtt ggcgataaga cttcgatcat tatcgctccc ctcgtggcgg cctgtggtgt
      421 ttgtgtgccg atgatctcag gtcgtggtct cggtcacacc ggcggcacgc tcgacaagct
      481 cgaatcgatt ccgggatatc gcgttgcgct cagtgcgagt gagttcaaaa cagtcctcga
      541 aaaggttggt tacgcgatgg caggacaaac tgccgaactc gcgccggcag acaagaagat
      601 gtacgcgcta cgcgacgcga ccgcgacagt cgaagccata cctttgatcg ttgcttcgat
      661 catctcgaag aagggggctg ccggactcga tgcgatgatc atcgacgtga aagtcggcaa
      721 cggtgcgttc atgcgccaag aggacagggc cagggcgttg gcccatgcgc tggtcagcac
      781 tggaaattcg tgtggcatca agacgcgcgc tctgttgacg gacatgaacc aaccactggg
      841 tggcgcggtt ggaaattcgc tcgaagttga agagtgcatc aacatcctgc gcggcgaggc
      901 gaatgaagcg gcggcgccgg tgctcgagtt gtctctcgaa ctctcggcgc acatgctggt
      961 gctcgcaaac gtcgacaaga cgatcgaggc ggcgcgccaa cgtttgcagg gcgtgttgga
     1021 ctcgggcaag gcgttggaat gcctgcaaag caacatcgag gcgcagggtg gcgatccgcg
     1081 tgtttgcgat gcgccgggcg attttctgcc tttggtaagc gaaaccgtta aggtagaatc
     1141 cccgcgctct ggcttcatca caaaggtcga taccacggag attggtcacg cgattgctgc
     1201 catcggtggc gggcgcgtgc gcatcgaaga cacggtggat ccgacggttg gttttacatc
     1261 cgatttgaag ttaggtgatc agatccgcgc cggcgacgcg atcgggacgg tgtactgcgc
     1321 cgacgcagcc gcagccgacg aggccgcgcg acgcattcaa gctgcttatc acgtcagtga
     1381 cgaagcgccc gcggagctga agttagttaa ggaaatcatc aacaaatgaa attcaagctt
     1441 gtcctggtag tcctatttat cgcgctcacg tcattgttgt tgtcaggttg tggcggcagc
     1501 tccgctgccg aagatccaac gaaaataaaa gtcgggattg tgtttgatat cggcggtaag
     1561 gacgatcgat cgttcaatgc tgctgcgtgg gaaggtgttc agcgtgccgc acgcgatctg
     1621 ccgatcgttt tgcgtgacat cgagccggga actccgaatg ccatcgagcc ggctatgcgc
     1681 gctttcgctg agcgcaattt cgatctcatc atcggcgttg gtttcgcgca ggcgccgatc
     1741 atggagctgg tcgcaaaaga ttatccgcac attcagttcg ccatcatcga cggcgtcagc
     1801 aaactcccaa acgtcgcgtc gctggttttc aaagaacacg aaggctcgta cctcgtcggc
     1861 atactcgccg ccaaaagttc acgcaccggc acgatcggtt ttctcggcgg catggacatt
     1921 ggtctgattc atcgtttcgc taagggctac gaagagggcg ccaaatccgt gaatccaaac
     1981 attcgcgtga tcgagaacta cgtaggcgtg actgacagcg cgtggaacaa cccgggcaaa
     2041 ggaaaggaac tgtcgttggc ccagatcggc aaaggcgctg acgtgatctt caccgccgcc
     2101 ggcaattccg gactcggcgc tttcgacgcc gtcgagcaac agggaaaaca gaatgggcgc
     2161 gcgacacatt tcgtgatcgg cgttgattcc aatcagaaca tggtcaaacc gggttttgtt
     2221 ctgaccagca tggtcaagcg tgtcgacaac gtggtctacg acatcgtcaa ggaggtcgtg
     2281 gaccacaaat tctcacccgg cgtgcatgtt ttcggtttgg acagagacgg cgtcggctac
     2341 gcgatggacg aatacaacaa agacctcgtt actcccgagg ccactcagca agctgaagaa
     2401 gcgaagaaga aaatcattgc gggtgaaatt aaagtgactg atgcgattgg tagttaatgg
     2461 tc
//
LOCUS       DASTEY010000083         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_199389, whole genome shotgun sequence.
ACCESSION   DASTEY010000083 DASTEY010000000
VERSION     DASTEY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199389"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1511)
                     /locus_tag="VFO72_01440"
     CDS             complement(255..1511)
                     /locus_tag="VFO72_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9477976.1"
                     /translation="MSFTQTLLRDRSIDNNRRQIRIQRKRLDQTDADFRQRTIDVITR
                     VQQAYWELVFALRDQQNQLANLNLSRESLRNVEAQIAAGARAPLERAEVQTEVANRET
                     ALLTAVQNVAITENNLKILIFRDPNSPEWSAQLTPTESPKFDTTPVNLTDALKAAREN
                     RPELRRLRLQNDINDIDIQFFKNQTKPQIDLVGTLALTGLAGKDVSGALPPGTQVPLI
                     FGNPQTNSTAFLFSQIQAIQIAQGLPVATSPLVNAQGGPNPTLIGGYGQTLRNLVGGD
                     TRNITVGVSISFPLKNKTAEANLAGARIQKEQLQASTRAQEQIVEVDVRNAAQAVESA
                     RQRVLSAREARVNAELQLEGEQRLYQVGRSTTFLLFQRENALANARASELRAETDYNK
                     AIADLQRATSTTLRANNVVVETPVEP"
     gene            complement(1512..2207)
                     /locus_tag="VFO72_01445"
     CDS             complement(1512..2207)
                     /locus_tag="VFO72_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477977.1"
                     /translation="MGTTAAVAQVPQSTPRPTPPQGDPTRPPGQEPIPGNPLPQNPTA
                     PPGPVRTSPTAVPGATPVPGATPVPQQPAGTVPGGETQEPIREPNIPPFQPRPLPPIP
                     SLTRLGVGTESIPLSLNDAIKRALENNNDIEIARDDVRFAETQLRALEGIFDPVFSIA
                     PQYDKRISPQQSSLGGSGQTGTTSTTTYSWNPAIVRQFGRGGGNYFLQFQNQHTNTSN
                     TFSLLNPFYSSNL"
     gene            2569..>2993
                     /locus_tag="VFO72_01450"
     CDS             2569..>2993
                     /locus_tag="VFO72_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HET9477978.1"
                     /translation="MAVKVGINGFGRIGRNIFRTALGDPDIDFVAVNDLTDTRTLAHL
                     LKYDSVLGNLDHEITATENGIKVDNEEFRVFSQRDPAAIDWESVGAEVVIESTGLFTK
                     AEDARKHLRGTVKKVIISAPAKNEDITIVLGVNEEKYDPA"
BASE COUNT          635 a          851 c          866 g          641 t
ORIGIN      
        1 tgattcttcg ttgaaagtga tgatagtggc gctgatcttc atctgctgac cacaaagaag
       61 cacaaaaagc acaaaaagcg caacaatgtt gcgctttcta tggtgatgca agtttgaacc
      121 ggcgccgatt ccgggacgtg ctcttccctg ggaccgcagg cgtctcgcct gctggtcacg
      181 agagcgcccg attcgtcacc gtccaggcga gacgcctgcg gtcccaggga agaggacgcc
      241 caagtaaacg gtcattatgg ttcgacgggt gtctctacga ctacgttatt ggcccgcaac
      301 gtcgtcgacg tagcacgctg taagtcagcg atcgccttgt tgtagtcagt ctccgcgcgc
      361 aactccgatg cacgcgcgtt cgccaaagcg ttttcccgtt ggaacagaag gaacgtcgtc
      421 gaacgcccga cctgatacaa acgctgctca ccttccagtt gcaactcggc gtttacacgc
      481 gcctcgcgag ctgaaagcac tcgctgacgt gcagactcaa ccgcctgcgc cgcattgcgt
      541 acgtcgactt caacgatctg ttcctgcgcc cgcgtcgatg cctgcaattg ctccttctga
      601 attctggcgc ctgcaaggtt tgcctccgcc gtcttgttct tgagcgggaa ggagatcgac
      661 acaccaaccg taatgttgcg cgtatcgccg ccaaccaggt tccgcagcgt ctgtccataa
      721 ccgccgatca gcgtcggatt cggtccgccc tgcgcgttca ccagcggcga ggtcgccacc
      781 ggcagaccct gcgcgatttg gatcgcctgt atttggctaa acaggaacgc agtggagtta
      841 gtttgcggat tgccaaagat caacggtacc tgcgtgcccg gcggcagcgc gccggaaacg
      901 tctttacccg cgagaccggt gagtgcaaga gtgcctacga gatcaatttg cggcttggtt
      961 tggttcttga agaactggat gtcgatgtcg ttgatgtcgt tttgcagacg caaccggcgc
     1021 agttccggtc gattctcccg cgctgctttc aacgcatctg tcagattcac cggcgtcgta
     1081 tcaaacttcg gcgactccgt cggcgtaagt tgagcagacc actccggact gttcgggtcc
     1141 ctgaagatca ggatcttgag attgttctcc gtgattgcga cgttctgcac cgcagtgagc
     1201 aatgccgtct cgcgattggc cacctccgtt tgcacttcag ctctctcgag cggagctctc
     1261 gcgcccgccg cgatctgggc ttcaacgttg cgcaagctct cgcgtgacag attcaggttg
     1321 gccaactggt tctgctggtc gcggagtgcg aacaccaact cccagtacgc ctgctgcacg
     1381 cgcgtaatca cgtcgatcgt gcgttggcgg aagtctgcgt ctgtctggtc caaacgtttg
     1441 cgctgtatgc gaatctgccg gcgattgttg tcgatcgagc gatcgcgcaa cagcgtctgc
     1501 gtgaacgaca attacaggtt tgaagagtaa aacgggttga gcagactgaa cgtgttgctt
     1561 gtgttcgtat gctggttttg gaactggagg aaatagtttc caccgcctct cccaaactgt
     1621 ctcactatgg ccggattcca tgaatacgta gtcgtactgg tcgtaccggt ttgtcctgag
     1681 ccgcccagcg agctctgctg cggtgaaatg cgtttgtcgt actgcggcgc gatcgagaag
     1741 actggatcga agataccttc gagcgccctt aactgcgtct ccgcaaagcg aacgtcgtca
     1801 cgcgcgatct cgatgtcgtt attgttctcg agcgcgcgtt tgatcgcgtc gttcagcgac
     1861 agcggtatcg actcagtgcc cacgccgagt cgggtcaagc tcggaatcgg cggcaatggt
     1921 cgcggttgaa acggcggaat attcggttcg cgaattggtt cctgcgtctc gccgcccggc
     1981 accgtacccg cgggctgttg cggaacggga gtagctcccg gcaccggagt cgctccggga
     2041 acagcagtcg gacttgtccg cacgggaccg ggaggcgcag tcgggttctg cggcagcgga
     2101 tttccgggga ttggttcctg gcccggcggc cgcgtcggat cgccctgcgg cggagtcggt
     2161 cgcggcgtgg actgcggcac ttgcgcgact gcggcagtcg ttcccacgag gacaacgcct
     2221 gcgagaacca cagtgctaac taacgttcga gaaagaaaag taaacatcag acgaagagct
     2281 ccttaaaaaa aggtggttgc atccagttcc cactacgtgg tgaagggaaa gtccgcaagc
     2341 attctggcaa accaaggtcc gccgcagcaa gactttacta cgttaccagc agaacggcag
     2401 tttcaagaaa tccgcccgca attccgcggc gatttattac taaacgatgg ctcgcttgac
     2461 actatgaaca gcaaaggcga gactttgtcc cacaatcgca acagttctaa tcccaaaaac
     2521 ggcacgactc gcagcgagtg tgcgcaaacg gcaggaggat tctcgagaat ggcagttaag
     2581 gttggtatca acgggttcgg acgcatcgga cgaaacatct ttcgcacggc tctcggcgat
     2641 ccggacatag acttcgtagc tgtgaacgat ctcaccgaca cgcgtaccct cgcgcacttg
     2701 ctcaagtacg actcggtact cggcaacctg gaccacgaga tcacagcgac ggaaaacgga
     2761 atcaaggtcg acaacgaaga gtttcgagtt ttctcgcaaa gagatcctgc tgctatcgat
     2821 tgggaatctg taggcgctga agtggtgatt gaatcaaccg gactgttcac gaaagccgaa
     2881 gacgcgcgca agcacctgcg gggcaccgtg aaaaaggtga tcatctccgc gccggcgaag
     2941 aacgaagaca tcacgattgt gctcggagtg aacgaggaga aatacgatcc ggc
//
LOCUS       DASTEY010000084         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_628178, whole genome shotgun sequence.
ACCESSION   DASTEY010000084 DASTEY010000000
VERSION     DASTEY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628178"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1335)
                     /locus_tag="VFO72_01455"
     CDS             complement(46..1335)
                     /locus_tag="VFO72_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET9477979.1"
                     /translation="MCTDRRDFLRLSAGVAGATLLGRSFSYADTESVEAAPQATQPEP
                     IRRLRRMTENVVPITLDERKARIEKAQRLMREQRIDAIYIEPGSSMFYYTGMRWGTSE
                     RMFAVVIPARGELAWICPKFEEERARELIRIGNDIRTWEEDESPYQRVAELLRDRGIR
                     TGTIGIEERVRFFLYDGIRLAAPALKFVNATPVTAGCRMFKSPAELALMQIANDITIV
                     AYKATRDSMTEGMTQGQFAANCSAAFRALGTSGGIFVSFGKYTAFPHGSSTPQRLKEG
                     DVVLMDGGCSVDGYQSDITRTFVFGKPTDRQRQIWDLERKAQDAGFAAAKVGAPCEAV
                     DAAARKVITDAGFGPDYKVPGLPHRTGHGIGLDGHEWTNFVRGNKTPIQPGMCFSDEP
                     TVVIYGEFGIRLEDCLYITENGPKFFTKQSASIDEPF"
     gene            1397..1987
                     /locus_tag="VFO72_01460"
     CDS             1397..1987
                     /locus_tag="VFO72_01460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET9477980.1"
                     /translation="MRSTDLHGSAPDKSNAALLLIDVINDFDFPEGEQLLRLAMPAGK
                     NIARLKQRAKAAGIPAIYVNDNFGRWRSDFKATVAHSREKGRGKEFVELLLPEDDDYF
                     VLKPKHSGFYSTTLSLLLTHLTAKNLILTGIAGNNCVLVTANDAYMRDFKLFVPADCV
                     ASNTEDENLHALKQMETVLKADTTIAAELDLQKISG"
     gene            2016..>3032
                     /locus_tag="VFO72_01465"
     CDS             2016..>3032
                     /locus_tag="VFO72_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9477981.1"
                     /translation="MCSLCAFPVRAQETLRWGADPSGGAPYVFTDPANPDSYIGYEKE
                     MVDALAAAMGRRAEFVPSDWETLVSALQRRSFDVIVNGLEPTGDRAQQILFSKPYYIF
                     QLQLTVRSDDNRISSLADCKDRVVGTLANTAASRLLQQERIPFRSYADPVGAYRDLEL
                     KRLDAVLMDVPAEAFYARPNAKLKPAGEPFTRGLYVIGLRKGDDALKAEVDNAIDKII
                     RDGTLETILKKWGLWNEAQVEFQTPIREGTHAQTNVEYTTTNATFNWSEALWRLARAA
                     GVTVLIALGSMVIAILLGLPLAVGQWKGPAWLRALCTVYVEFFRGTPVLVQLLFLYFG
                     LPLIG"
BASE COUNT          638 a          891 c          858 g          645 t
ORIGIN      
        1 ttttggggca aagcactgtc cgtattgttg tggaagttcg tggccctaga acggttcgtc
       61 gattgaagcg ctctgtttgg tgaagaattt cggcccgttt tcagtgatat acaggcagtc
      121 ctcgagacgg atgccaaact cgccgtaaat cacaaccgtc ggttcgtcgc tgaaacacat
      181 accaggctgg attggagtct tattgccccg cacgaagttg gtccattcgt gaccatcgag
      241 accgatgccg tggccggtgc gatgcggcag gccgggaact ttgtagtccg gaccgaaacc
      301 agcatccgtg atcactttgc gggcagccgc gtcgaccgct tcgcatggag caccaacttt
      361 cgcggcagca aaaccggcgt cctgcgcttt gcgttcgaga tcccaaatct gtcgctgccg
      421 atcggtcggc ttgccaaaca caaacgtgcg cgtgatgtct gactggtaac cgtcgacact
      481 gcaaccaccg tccatcagca cgacgtcacc ttctttcagt cgctgcggtg tgctgctgcc
      541 gtgtggaaac gcggtgtact ttccgaagct gacgaaaatt ccgccggacg taccaagcgc
      601 gcgaaacgcc gccgagcagt tggcggcaaa ctggccctgc gtcatgcctt cagtcatgct
      661 gtcgcgcgtc gctttgtaag ccacgatcgt gatgtcgttg gcgatctgca tcagcgcgag
      721 ttctgccgga gacttgaaca tgcggcaacc ggcagtaacc ggcgtggcgt tcacaaattt
      781 caaagcgggc gcagccagac gaataccgtc gtaaagaaaa aaccgcactc gctcctcgat
      841 gccgatcgtg ccggtgcgta taccgcgatc gcgtaacagc tccgccaccc gttgataagg
      901 gctctcgtct tcttcccagg tgcgaatgtc attgccgatg cggatcaact cgcgcgcgcg
      961 ttcttcttcg aatttcgggc agatccatgc cagctcgcca cgcgccggaa tcacgacggc
     1021 aaacattcgc tcgctcgtgc cccagcgcat gcccgtgtaa taaaacatgc tgctgcccgg
     1081 ctcgatgtag atggcatcga tgcgctgttc gcgcatcagt ctttgcgctt tctcgatccg
     1141 cgctttacgt tcgtcgagtg tgatcggcac gacgttctct gtcatgcgac gcagccggcg
     1201 aatcggttcg ggttgggtag cttggggtgc tgcttcgacc gattctgtgt ccgcgtaaga
     1261 gaacgatcgg cccaacagcg ttgcgccggc cacgccggcg gataagcgaa ggaagtctcg
     1321 gcgatcggtg cacatggtga cacaaattac aacatgatgg ttgagagtga aaggggtagg
     1381 ggatagaatc cgaccggtga gaagcacaga tctacacgga tcggcgcccg ataagtccaa
     1441 cgcggcgctg ctcctgatcg acgtcatcaa cgacttcgac tttccggagg gcgaacaact
     1501 gctgcggctg gccatgcccg ccggcaaaaa catcgcccgt ctcaagcagc gtgcgaaagc
     1561 cgccggcatc ccggcaattt atgttaacga caacttcggc cgctggcgat ccgatttcaa
     1621 agcgacggtc gctcatagcc gcgaaaaagg gcgcggcaaa gagttcgtcg aactactcct
     1681 tccggaagac gacgactact tcgttttgaa accaaaacat tccggcttct attcaacaac
     1741 gctttcgttg ctactgacgc acctcacggc caaaaacctg atcctcaccg gtattgccgg
     1801 aaacaattgc gtcctggtta ccgccaacga cgcgtacatg cgcgacttca agctcttcgt
     1861 cccggcggat tgcgtcgctt caaacaccga ggacgagaat cttcacgcct taaagcagat
     1921 ggagacagtg ttgaaggctg ataccactat agccgcggag ttggaccttc agaagatcag
     1981 tggctaacgc ccggctcatc atcatagcgg tcctcttgtg cagcctgtgt gcgttccctg
     2041 ttcgcgctca ggagacgcta cgttggggcg cagatcccag cggtggcgcg ccttacgtct
     2101 ttaccgatcc cgccaatccg gatagttaca tcggctacga aaaggagatg gtcgatgctt
     2161 tagcggcagc catgggccgg cgcgcggagt ttgtgccgag cgactgggaa acactcgtct
     2221 ccgcactgca acggcgatcg ttcgacgtga tcgtcaacgg cctggaaccg acaggcgatc
     2281 gcgcacagca aatactcttc agcaaaccgt actacatttt tcagctccag ctcactgttc
     2341 gcagcgacga caaccgcatc tcgtcactgg cagattgcaa ggaccgcgtc gtcggtacac
     2401 ttgcaaacac cgccgcttcc cgacttctcc aacaggagcg aattcccttt cgcagttacg
     2461 cggatccggt cggcgcctat cgcgatctgg aactcaagcg cctcgatgcg gtgctgatgg
     2521 acgtgccggc ggaagcgttc tacgcccggc ccaatgcgaa gttgaaacct gccggggaac
     2581 cgttcacgcg cggcctctac gtcatcggtt tgcgcaaagg cgacgacgct ttgaaagccg
     2641 aggtcgataa cgcgatcgat aagatcattc gcgacggtac actcgaaacg attctcaaaa
     2701 agtggggact ctggaacgaa gcacaggtcg agtttcaaac gccgattcgt gagggcacgc
     2761 acgctcaaac gaacgtcgag tacacgacca cgaacgccac gttcaactgg agtgaggcac
     2821 tttggcgttt ggcgcgggct gctggtgtca ctgtcctcat cgcactcgga tcgatggtga
     2881 tcgcgattct tctcggatta ccgctcgctg tcggacagtg gaaaggacct gcgtggctgc
     2941 gagcattgtg cacggtgtat gtcgagttct tccgcggcac gccggtgctg gtccaactgc
     3001 tttttcttta cttcgggttg ccgttgattg ga
//
LOCUS       DASTEY010000085         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_446701, whole genome shotgun sequence.
ACCESSION   DASTEY010000085 DASTEY010000000
VERSION     DASTEY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446701"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VFO72_01470"
     CDS             <1..1638
                     /locus_tag="VFO72_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477982.1"
                     /translation="RAGVVLWDTDPERARELFRRAWEAAEIVDTEAAKKRAEEMKRLE
                     SEGGPVVVRGGPDLRSEVLRLVAKRDRKLGEEFLKALDEAAEKARTEAAADQRRNTGM
                     RLGASQRLQLARRLVEDGEVERALEFATPSLDSVNVDSIYFLSALREKNAQLADAAFS
                     NLLARAARDPNSDANTVSGLSSYLFTPFLYVTFERNGGANQNRQRGPTPAPPVNSGLL
                     NSFLKLGFQILMQPLAAPDQDRTTSGQTGKYLVIKRLLPIFEQFAPDLAPGLRTQMTA
                     LASYVPSTFQQGENRAVTVGLQPESSEGDPYQRMQERLDRAGKSEDRDAIYADYAVVF
                     AGDGDPRARDLVDKIENTELRKSVKAYTDFQLVQSAIRKKDVTEAVRLVKNGDLTSIQ
                     KVWALTSAAKLVMDAERSRATDMLEEALTESRRISGSDPDRARALTAVAVGFGEMDRV
                     RAWEILGEVVKAANGAEDYTGEDSRISSRLQTKFMVVMTNSTSEDFDLLAVFRHLARA
                     DLLRAVQLAKTFTGEAPRAVATLAIARSVLEKRKDPV"
     gene            1727..>2043
                     /locus_tag="VFO72_01475"
     CDS             1727..>2043
                     /locus_tag="VFO72_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477983.1"
                     /translation="MKHFHLLLCLLFTATVTQAQVAKTAPPAKSAAMTEEELKARERR
                     VRARALLVSLSTDARQFHDQTLRARSLARIADALWRVDAEQARLLFRKAWDAAEVADQ
                     ENAR"
BASE COUNT          458 a          605 c          596 g          384 t
ORIGIN      
        1 cgcgccggag ttgtgctttg ggacacagac cctgaacgcg ctcgcgaact cttccgccgc
       61 gcctgggaag ctgctgagat tgtcgacacc gaggcggcaa agaaacgcgc cgaggaaatg
      121 aagcgactcg agagcgaagg cggtccagtc gtcgtgcggg gtgggcctga cttgcgcagc
      181 gaagttttgc gcctggtcgc aaagcgagat cgaaaactcg gcgaagagtt tctcaaagcg
      241 ctcgatgaag cagcggaaaa ggcacgcacc gaagcagcag ccgatcagcg ccgcaacacc
      301 ggaatgcgcc tgggcgcgtc acagcgcctg caactcgcgc gcaggctggt tgaagatggt
      361 gaagtagaac gcgcactcga gtttgccacg ccgtcactcg actccgtcaa cgtcgattcc
      421 atctattttc tttccgcact acgcgagaaa aatgcacaac tcgccgacgc agctttcagc
      481 aacttacttg ctcgcgctgc tcgcgatcca aactccgatg ccaacaccgt ctccggtttg
      541 tcgtcgtatc tcttcacgcc gtttctatac gtgacatttg aacgaaatgg cggcgccaat
      601 caaaaccggc aaagaggtcc gacgcctgcg ccgcctgtca attcgggact gctaaatagt
      661 tttctgaaac tcggcttcca gattctgatg caaccactgg cagcacccga tcaggatcgc
      721 acgacgtcag gacaaaccgg caaatacctg gtcatcaaac gcctgctgcc gatcttcgaa
      781 cagttcgcgc ctgatctcgc gccaggcttg cgcacacaaa tgaccgcgtt ggcctcgtac
      841 gtgccgagca ctttccagca aggcgagaac cgcgcagtca ctgtcgggct tcagccggag
      901 tcgtcagaag gcgaccctta ccagcgcatg caggaacgtc tcgatcgcgc cggcaagtcg
      961 gaagatcgcg atgcgattta cgccgactac gcggtcgtgt ttgcaggcga tggcgatcca
     1021 cgggcacgcg atctggtcga caagatcgag aacaccgagc tgcgcaaaag cgtcaaagcg
     1081 tacactgact ttcaactcgt ccaatctgca attcgaaaga aagacgtcac cgaagcggtc
     1141 cgacttgtta agaatggtga tttgacaagt attcaaaaag tgtgggccct gaccagcgct
     1201 gcaaaactcg tgatggatgc ggagcgttcg cgtgcgacag acatgctcga ggaagcacta
     1261 actgaaagtc gtcgcatcag tggcagcgat ccggatcgcg cccgcgcgct gactgcggtt
     1321 gcggtcggct ttggcgagat ggatcgcgtg cgcgcgtggg agattctcgg cgaagtggtg
     1381 aaggcggcaa acggcgcgga agactacacg ggtgaagaca gtcgcatctc ttcgcgcctg
     1441 caaaccaaat tcatggtggt gatgactaac agcacctccg aagattttga cttgctggcg
     1501 gttttcaggc atcttgcacg cgccgatctt cttcgagccg ttcagctcgc aaaaaccttc
     1561 accggagaag caccgcgggc tgtagcaacg ctggcgattg cgcgttcagt gctcgagaaa
     1621 cgcaaggatc ccgtgtaagc gttgtagggg tggccctccg tggccacccc agcgtggaca
     1681 ttccaacact ggggcggcca cggagggccg cccctacaaa aaatttatga aacactttca
     1741 tcttctgttg tgtttgctct tcactgcaac tgtcacgcaa gcacaagtag ctaagactgc
     1801 gccacccgcg aaatccgcgg ccatgaccga agaggagctc aaggcgcggg agcgtcgcgt
     1861 acgcgcacgc gcgctgctcg tgtcgctctc aaccgacgcc cgccagtttc acgatcaaac
     1921 gctgcgcgcc cggtcgctcg ctcgcattgc ggacgcgctc tggcgggtcg atgcggagca
     1981 ggctcgactg ttgtttcgca aagcatggga cgccgcggaa gttgccgatc aggaaaacgc
     2041 tcg
//
LOCUS       DASTEY010000086         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_117204, whole genome shotgun sequence.
ACCESSION   DASTEY010000086 DASTEY010000000
VERSION     DASTEY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117204"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFO72_01480"
     CDS             <1..565
                     /locus_tag="VFO72_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477984.1"
                     /translation="VEELAAQLELTDNAVRAHLATLERDGLVRQSGLRRGPRKPHFTY
                     VLTPEADALFPKAYDALLNQLIAVLKNRLKPSEIEDVLREVGRAVAAGAPSGENNLNG
                     RVQTALKVLEAIGGSAAVEQHEDKIVIQGNSCPLAAAVNVHPEVCQLAETLVAEIVKA
                     PVHETCDREGRPKCRFEISLKEAQKSK"
     gene            648..1949
                     /gene="serS"
                     /locus_tag="VFO72_01485"
     CDS             648..1949
                     /gene="serS"
                     /locus_tag="VFO72_01485"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9477985.1"
                     /translation="MLDLNYVRENIDKVREALTARRADSTALDDFTRADEERRRVIAE
                     SDQLNAQRNASSREIGGLMKEGKKEEAEARRADVAKLKDRISELDELRTQAEARMHEL
                     LSTLPNIPHATVPVGEDESANQEVRRWGTPPKFDFEPKDHVDLATALGILDLERATKI
                     TSARFAILNGAGARLERALINFMLDVQTREHGYLETLPPFIVNRASLFGTGQLPKFET
                     DLFKLQDDRELYLIPTAEVPVTNYHRDEILDASHLPMRWAAYTPCFRSEAGSYGRDTR
                     GVIRQHQFEKVELVKYALPENSYEELESLTRDAETILQKLGLHYRVMTLSTGDLSFGS
                     AKTYDIEVWLPSQNTFREISSCSNCESFQARRAQIRFRRAGGAKPEFVHTLNGSGLAI
                     GRTWIALLENYQQEDGTVIVPEVLRPYMGGIDRIRAADERG"
     gene            complement(1996..>2601)
                     /locus_tag="VFO72_01490"
     CDS             complement(1996..>2601)
                     /locus_tag="VFO72_01490"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET9477986.1"
                     /translation="KIISQPSTITGSIGVVAGKPVLRGFYDWLGVSNEYVLRGKTAGM
                     FRETEKFSDEERVKFEEWIKTTYYQDFVPKVAKGRQKDPQYIDSIAQGRVWTGQQAKD
                     RGLVDEFGGLDKAIEIAKQLAKIPADKGVERVILPYPQTFLQQLMSGSNENTNTQVEQ
                     QRAVFAALPEDARRALRYMALLDRMKNGESMLLMPFDLRVK"
BASE COUNT          577 a          732 c          733 g          559 t
ORIGIN      
        1 agtcgaggag ttggcggcac agctcgagct gactgacaac gcggtgcgag cgcaccttgc
       61 aacgctcgag cgcgacgggc tggtgcgaca gagcggactt cggcgcggtc ctcgcaaacc
      121 gcactttact tacgtgctga cgcccgaagc tgacgcactc tttcccaaag cttacgacgc
      181 tctgctcaat caacttatcg cggtgctgaa gaaccggcta aagccgtcgg agattgaaga
      241 cgttctccgt gaagttgggc gcgcggtggc ggcgggtgcg ccgagcggag agaacaatct
      301 gaacggtcgt gtgcagactg cgctgaaggt gttagaggcg atcggtggat ccgccgcagt
      361 cgagcagcat gaagacaaga tcgtgattca aggtaacagc tgtccgctgg ccgccgctgt
      421 aaatgttcat ccggaagtct gccagttagc cgagacgctt gtcgcggaga tcgttaaagc
      481 cccggtccat gagacctgtg atcgcgaagg gcgcccgaag tgtcgcttcg agatcagcct
      541 caaagaagca caaaaatcga agtaggccac aaaaaggcac aaaatgaaac caagatcttt
      601 tttgtgcctt ttgtgctttt ttgcggctta agatactttt gtggcctatg ttggatctta
      661 attacgttcg cgaaaatatt gataaggtgc gggaggcgct cacggcacgc cgggccgaca
      721 gcaccgcgct cgacgatttc acgcgcgccg atgaagaacg gcgtcgcgtc attgctgaat
      781 ccgatcaact gaatgcacag cgcaacgcgt cgagtcgtga gatcggcgga ttgatgaaag
      841 agggcaagaa ggaggaagca gaagcgcgcc gcgctgacgt cgcgaagctg aaagatagga
      901 ttagcgagct cgatgaactg cgcacgcaag ccgaggcgcg catgcacgaa ctgctttcga
      961 ctcttcccaa tatcccacac gcaaccgtgc ccgtcggcga ggacgaatcg gcgaaccagg
     1021 aggtgcgtcg ctggggcaca ccgccgaagt ttgattttga accgaaggac cacgtcgatc
     1081 tcgccacggc actcggcatt ctcgatctcg aacgcgcaac caagatcact tcggcgcgct
     1141 ttgcgattct caacggcgcc ggcgcccgcc tcgaacgcgc gctgatcaat ttcatgctcg
     1201 acgtccaaac tcgcgagcac gggtatctcg aaacactgcc gccgttcatc gtcaaccgtg
     1261 cttcactctt cggcaccggg cagttgccaa agtttgagac ggatcttttc aagctacagg
     1321 acgatcgcga gctctacctg atacctacag ctgaagtgcc ggtcactaac tatcatcgcg
     1381 acgagatact cgacgcttca cacctcccga tgcgttgggc cgcatacacg ccgtgttttc
     1441 gatctgaagc gggtagttac gggcgcgaca cgcgcggtgt gatcaggcaa caccagtttg
     1501 agaaagtcga attggtgaag tatgcgttgc cggagaattc ttacgaagag ctcgaatcgt
     1561 tgactcgaga cgcggagacg atcctgcaaa agctcgggtt gcactatcgt gtgatgacgc
     1621 tctcgactgg cgatctcagt ttcggatcgg cgaagaccta cgacatcgaa gtgtggctgc
     1681 cgtcgcagaa cacgtttcgc gagatctctt cgtgctccaa ctgcgaatcg tttcaggcgc
     1741 gtcgcgcgca gatccgtttc cgtcgcgcag gcggagcgaa gccagagttt gtacacacac
     1801 tcaacggctc tggcctggca atcggtcgca cgtggatagc tctgctggag aactatcagc
     1861 aggaggatgg aacggtgatt gttcctgaag tcctccgacc gtatatgggg ggtatagaca
     1921 gaattagagc cgcagatgaa cgcggatgaa cgctgattct gatcagcgtt tatttgcgtt
     1981 tatctgcggc ttctattact tgactctcag atcgaacggc atcaacagca tcgattcgcc
     2041 gttcttcatt cgatccaata acgccatgta tcgcagcgca cggcgcgcgt cctcaggcag
     2101 cgcggcgaac acggcgcgct gctgttcgac ctgagtgttt gtgttctcgt tgctgccact
     2161 catcaactgc tgcaggaacg tttgcggata aggcaggatc acgcgttcga cgcctttgtc
     2221 agcgggaatc ttcgcgagtt gtttcgcaat ctcgatcgct ttatcgagcc cgccaaattc
     2281 atccaccagg ccacggtcct tcgcctgctg tccggtccac acgcggccct gggctattga
     2341 gtcgatatac tgcggatctt tttggcggcc cttcgctacc ttcggtacaa aatcctgata
     2401 gtaagtagtc ttgatccact cttcaaactt cactcgttcc tcatcggaaa acttttccgt
     2461 ctcgcggaac attcccgcgg tcttcccacg caacacgtac tcgttcgaga ccccgagcca
     2521 atcgtaaaaa ccacgaagca cgggtttgcc tgcgaccacg ccgatcgaac ccgtaatcgt
     2581 cgatggctgt gagatgatct t
//
LOCUS       DASTEY010000087         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_430401, whole genome shotgun sequence.
ACCESSION   DASTEY010000087 DASTEY010000000
VERSION     DASTEY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430401"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFO72_01495"
     CDS             <1..384
                     /locus_tag="VFO72_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9477987.1"
                     /translation="KAAYVFYGNAPMNAQRGVDKEALARITAPVYGFYAGNDARINET
                     LPATSQAMTELKKTFEPVTYEGAGHGFMRAGDAPEPVAPTPKGDQAADEKAKADYEKA
                     LAQWKANKKARDEAWVRWKKLLASV"
     gene            complement(376..1344)
                     /locus_tag="VFO72_01500"
     CDS             complement(376..1344)
                     /locus_tag="VFO72_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET9477988.1"
                     /translation="MSIVLLLQAHGQLTSRTLAERLEVSERTIHRDMEALSFSGIPVV
                     AERGSNGGWSLLGEYRTNLTGLNEAEIQSLFVTQPPKLLADLRLEKASEGALLKLLAA
                     LPAMYRRGAEQARKRIHIDTAGWSRQEESVPLLPVLQEAIWSERKLRFSYQRGPGCDD
                     VEREVDPLGLVAKGSAWYLVAGIAGDIRSYRISRVSRAAVLDQPCTVPPDFDLAVFWE
                     QSTTAFKSTLPNYMAKFRVAPEVFPRLRFAGRFARVGEVVETDLHGWYVVPVGFDVEE
                     IACEYALSFGSNLEVLEPRSLREKVIAAAQQVVNFYLEPQKNTDLH"
     gene            1443..1961
                     /locus_tag="VFO72_01505"
     CDS             1443..1961
                     /locus_tag="VFO72_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9477989.1"
                     /translation="MRKVIFGGANTLDNYIAGKNDSIDWIRHNKEVDEIMKSYWQTID
                     TVILGRRTWEIAMKGSSGGSKGIKSYVCSRTLKKIPGKKIQLEPDAVSLVKRLKSEEG
                     KDIIVMGGSLIGKSLFEAGLIDEFGVNIHPVLLGSGIPLFCEMDRRIDLELLECRQLK
                     NGCVFLNYRVLP"
     gene            complement(2136..3680)
                     /locus_tag="VFO72_01510"
     CDS             complement(2136..3680)
                     /locus_tag="VFO72_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9477990.1"
                     /translation="MMLPLMILSLVFAVSAAGGDEPQTQTVTTTVQSFTAVEGADLMT
                     KLEAAQARGRTGQTPYWSAYTFDVRPGVAVDPAVREFRGNMNTIGDTTVFVGTDASGV
                     TVETRNLAIFVLREPSGNQITRMEIYNLERKREYSGYPVYWLGRANNEESLNYLRAIA
                     AATPLDRLSERAVLGIALHDDSRVGGMLRNFVVSSPNPRIRSTAVYWMGQTGGEQVFL
                     ASLVRNDTEDRKLRRSAAHAIGQSHDRGTMATLQSLYQDVKDPEIRRSVIAAAGSSDE
                     EQPAFTFLLGVAKTDADWESRRTAVRQLGHFQREDAAGELIKIYGSDANIEVKKAALR
                     SLSETKSPKALARLLEIARSDANGELRKQAIRVLGQRGEAVVDDLLKIFDTERMPDVR
                     RAALHSLSQIKSTRVEDKLFEIARGNEPVDMKRQAIRMLGERVSKRSFDFLSSTAQGT
                     DANAEVQVQAVRAISQRRSDESVPLLIKIARTHPNLLVRKQAIRSLGESGDPRAIEYF
                     REVLSK"
     gene            complement(4159..>4929)
                     /locus_tag="VFO72_01515"
     CDS             complement(4159..>4929)
                     /locus_tag="VFO72_01515"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9477991.1"
                     /translation="TPCQGFQSAAVSLLGAHGGARAVPILLDVARSNADQKLRLTAIK
                     RLGEQRNEQVTDELIKIYDTDQNRDVKIQALRAIAESGTTRGNAKLLDIARSEGDQAV
                     REWAISRVGQMKGDAALDELLRIYDGAKTMEIRSRVVRALLERRDDPRARAKVWDVAR
                     KDPMPELRSYAIRNLRGDDDEQAMVQFIALYDAEQSQLVKAVLIRGLSMSKHQVAIRK
                     LMTIARSDPSVELRKTAIRYLGQSKDPEALKFLEDLLK"
BASE COUNT         1104 a         1416 c         1331 g         1078 t
ORIGIN      
        1 aaagccgcgt acgtcttcta cggcaacgca cccatgaacg cccagcgcgg tgtcgataaa
       61 gaggcactcg cgcgaatcac agcgccggtc tacggttttt acgccggtaa cgacgcgcgt
      121 atcaacgaaa ctctaccagc cacatcacaa gcaatgaccg aactgaagaa gacgtttgag
      181 ccggtcactt acgaaggcgc cggccacggc tttatgcgtg ccggcgacgc gccggaaccc
      241 gtcgcgccaa ctccgaaagg agatcaagcg gctgacgaaa aagcaaaagc cgattacgag
      301 aaggctttgg ctcagtggaa ggcaaacaag aaagcacgcg atgaagcgtg ggtgcgctgg
      361 aagaaactat tagcctcagt gtaaatccgt gttcttctgt ggctctaaat aaaagttcac
      421 tacttgttgc gcagccgcga tcactttctc ccgcaacgat cgcggctcga gcacttcgag
      481 gtttgaacca aacgagagcg cgtactcaca cgcgatctct tccacatcaa aacccaccgg
      541 cacgacatac cacccgtgta aatccgtctc cactacctcg ccgacgcgag cgaatcgtcc
      601 cgcaaaccga agccgcggaa aaacttccgg cgctacgcga aacttcgcca tgtagttggg
      661 caatgtcgac ttgaacgcgg tcgtcgattg ctcccaaaac accgcaagat caaagtccgg
      721 cggaaccgtg cacggctggt ccaacactgc cgcacgcgag acccgcgaaa tgcgataact
      781 gcgaatgtct cccgcgatac ctgccaccaa ataccacgcg cttcccttcg caaccaatcc
      841 gagtggatcg acctcccgtt caacgtcgtc acaacccgga ccacgttgat agctgaaacg
      901 aagtttacgt tcggaccaga tcgcttcctg caacaccggc agtaacggca ccgactcttc
      961 ctgtcgtgac cagcctgccg tgtcgatgtg aatacgcttg cgcgcctgtt cggccccgcg
     1021 gcgatacatt gccggcagtg cggcgaggag tttcaggagt gcgccttcgg atgctttttc
     1081 cagcctgaga tcggcaagca gtttgggtgg ctgggtaacg aagagagatt gaatctcggc
     1141 ttcgttcagg ccggtgagat tcgtgcgata ttcgcccagc aacgaccagc caccgttgct
     1201 gccgcgctcc gcgacaaccg gaatccccga aaaactcaac gcctccatgt cccgatgaat
     1261 tgtgcgttca gagacttcca atcgttcggc gagtgtacgt gaagtcaatt ggccgtgcgc
     1321 ctgcaacaaa agaactattg acaataaacg gtcggcacgc atgtccgtga ttttaccgca
     1381 gaaacatgac acacgttgtc atatatgaag aagtaaattg ctcgaaggga ggtaactcga
     1441 caatgcgaaa agtgatcttt ggtggggcaa acacactcga caactacatc gccggcaaaa
     1501 acgactcgat cgactggatt cggcacaaca aggaagtaga cgagattatg aagagttact
     1561 ggcagacgat cgataccgtc attttgggtc gccggacctg ggagatcgcg atgaagggca
     1621 gttccggcgg ttccaaggga atcaaaagct acgtttgctc gcgcacgctc aagaaaattc
     1681 ccggaaagaa aatacaactc gagccggacg ccgtgagcct ggtgaagcgg ctcaagtcag
     1741 aagaaggcaa agacatcatc gtgatgggcg gcagtttaat cggcaagtcg ttgtttgagg
     1801 ccggattgat cgatgagttt ggggtcaaca ttcacccggt cctgcttggc tcgggtatac
     1861 cgctgttttg cgagatggac cggcgaatcg atctcgaact gctggagtgc cggcaattaa
     1921 agaacggatg cgttttcttg aactaccgtg ttcttccgtg aaactccgtg gcggcagtga
     1981 tgcagtttga aaattgaggc accttcctgc tgggaccgca ggcgtctcgc ctgcatggtt
     2041 tcgatagtag ttctctacgc tgctatgcag atgagacgcc tgcggtccca ggtaaaccgt
     2101 ccgtagtttc aacctgcatc accaatctct gtggcctatt tagacaagac ttcccggaaa
     2161 tactcgatcg cgcgcggatc gcctgattcg cctaacgagc ggatcgcctg ttttcgcaca
     2221 agcaaattcg ggtgcgtacg cgcgatttta atcagcaacg gcactgactc gtctgagcgg
     2281 cgctggctga ttgcccgcac cgcctgcacc tgaacttccg cgtttgcgtc agtgccctgt
     2341 gcggttgaac ttaggaagtc gaaactccgt ttactcacac gttcgccgag cattcgaatc
     2401 gcctggcgtt tcatgtcgac cggttcgttg ccacgcgcga tttcaaacaa cttgtcttca
     2461 acacgcgtgc ttttgatctg agaaagcgaa tgcaaagcag cgcgccgcac gtcgggcatt
     2521 cgctcggtgt caaatatttt cagaagatcg tcaacgacag cttcgccacg ttgacccaac
     2581 acacggatcg cttgcttgcg caactcgccg ttagcatcac tgcgcgcaat ttcgagtaag
     2641 cgtgccagcg ctttagggct cttcgtctcc gacaacgagc gcaacgcggc ttttttgacc
     2701 tcgatattcg cgtcgctccc gtagatcttt atcaactcgc cggcagcatc ttcgcgttga
     2761 aagtgaccga gttgtcgcac ggcagtgcga cgtgattccc agtcggcgtc agttttcgcg
     2821 acaccgagca gaaacgtaaa cgccggctgt tcttcgtcac tgctaccagc agccgcaata
     2881 acactgcgac ggatctccgg gtccttcacg tcttgataaa gactctggag agtcgccatc
     2941 gtgccacgat cgtgactttg tccaatcgca tgtgcggcgg aacggcgaag tttccgatct
     3001 tcagtatcgt tgcgcacgag cgaagcgaga aacacctgct cgccgccagt ctggcccatc
     3061 cagtaaacag cggtagagcg aatgcgtggg ttcggcgaac tcacgacgaa attcctgagc
     3121 atgccgccga cgcgtgaatc gtcgtgcaaa gcaatgccga gaacagcgcg ttcgctcaac
     3181 ctgtcgagcg gcgtcgcagc ggcgattgcc cgcaaatagt tgagactctc ttcattgttc
     3241 gcgcggccca gccagtaaac gggatagccg ctgtactcgc gctttcgttc gagattgtaa
     3301 atctccatgc gcgtgatctg gtttccggat ggttcgcgca acacaaaaat tgcgagattg
     3361 cgtgtttcta cggtgactcc actggcgtcg gtgccaacga acacggtcgt atcgccgatg
     3421 gtgttcatgt tgccgcgaaa ctcgcgaacg gcaggatcga ctgcgacgcc cgggcgaacg
     3481 tcgaatgtgt aagccgacca gtagggcgtt tggcctgtac gaccccgcgc ttgcgcggct
     3541 tcgagtttcg tcatcaggtc ggcgccttcg accgcggtaa aactctgcac ggtggtcgtg
     3601 actgtttggg tttgcggttc gtcgccgccg gccgccgaca ccgcgaacac caaagacaga
     3661 atcatcagtg ggagcatcat gacattcctc cggcttttcc tcggaccaca ggcgtctcga
     3721 ctacaacctg cgaagcagcc tgcttacgac cagtgcaggc gagacgcttg cggtcccagg
     3781 aaagaaggtc tatttagctt tgcaaagaaa agtacatctt tctgtgcttt gcctcaaaag
     3841 tcctctctca ttctcaccgg gctttagccc ggtgcaggag cagtgaagac ggaggaaacc
     3901 gtttcaacgg ttttccgttg attcgccagc gaaaccgtta aaacggtttc tcaaaccgcg
     3961 tggcggtaac accgggctga agccccggtg agaatgagag aagacttttg aggcaaagcg
     4021 atctttctga aagaactctc gcgcaaaggc gcaaagcggc taccgcgttt ttaagggttt
     4081 tctttgcgcc cttgcgcgag gcattttttt ccacagtggt ccacaaacgc catttaagtg
     4141 cttttcgtgc aaaccggtct atttcaaaag gtcctcgaga aatttcaacg cctcgggatc
     4201 tttgctttgg ccaagatatc tgatcgccgt cttacgcaac tccaccgacg gatcgctgcg
     4261 cgcgatcgtc atcagtttgc gaatggccac ttggtgcttc gacatgctaa gaccgcgaat
     4321 cagaacagct ttcactagct gactctgctc ggcgtcgtac aacgcaatga actgaaccat
     4381 ggcctgctcg tcgtcgtccc cgcgcaaatt gcggatcgca tagctgcgta gctccggcat
     4441 ggggtctttt cgcgcgacgt cccagacctt cgctcgagcg cgcggatcat cgcgtcgctc
     4501 cagcagcgct cgcacgacgc gagaacggat ctccatcgtt ttcgcgccat cgtagatgcg
     4561 gagcaattcg tccagtgcag cgtcgccttt catctggccg acccggctaa tggcccactc
     4621 gcgcactgcc tgatcgcctt cgcttcgtgc aatgtcgagc agcttagcat tgccacgcgt
     4681 ggtgccgctt tcagctatcg cgcggagtgc ttgtattttt acgtctcggt tttgatcggt
     4741 gtcgtagatc ttgatcaact cgtcagtaac ctgctcgttg cgctgttcgc cgagacgttt
     4801 aatggcagtg agtcgaagtt tctgatctgc attactgcgc gcgacgtcga ggaggatggg
     4861 tactgcacga gcgccgccat gcgcgcccag tagtgacact gccgctgact ggaaaccctg
     4921 acaaggcgt
//
LOCUS       DASTEY010000088         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_67946, whole genome shotgun sequence.
ACCESSION   DASTEY010000088 DASTEY010000000
VERSION     DASTEY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67946"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VFO72_01520"
     CDS             complement(<1..888)
                     /locus_tag="VFO72_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9477992.1"
                     /translation="MSTSAAQQPSPQLFFQTINAHQRTEALKAAIELEVFTAIGEGNT
                     TAAQIAQRCQTSEKGMRILCDFLTIMGMLTKEGDNYALTLDSATFLDKRSPAYLGGTV
                     EFMCSPLLTDSAKHIAEAVRKGGTVMDDQGTIGPENPVWVKFARGMGAMMALPAQLIA
                     KLVDPNADRKLKVLDIAAGHGLFGIAFATNNPQAEITAIDWKAVLEVAKENAQKAGVS
                     DRYNTIEGSAFDVEFGSGYDLVLLTNFLHHFDPPTCETLLRKVYSALAAGGRAVTLEF
                     VPNEDRVTPPDAAGFSMMML"
     gene            complement(941..2806)
                     /locus_tag="VFO72_01525"
     CDS             complement(941..2806)
                     /locus_tag="VFO72_01525"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9477993.1"
                     /translation="MKLRMPEMPPATLLSALDGYNLLPAIVFMPTRRRCDKAASEAAL
                     ARPTGSDPRREARREFMRSFVEQHPEVRGHRHWDTIIRGGVASHHAGHIPSWKLVIER
                     LMSAGLLDAIFATATVAAGVDFPARSVVLTGSDARTASGWRPLSASELQQMTGRAGRR
                     GRDNVGFVVAAPGLHQDPLRIAQLLKAPPDPLVSQFRATYTTLLNLLDAYGTFAHVRE
                     IAERSFAHRDSVRQLSQLEKSREQSAETIQRRLHDAGYDLPVSTVLGLERLIGARARL
                     QEAKPQTRAEVFSRWLNEVVKPGRVVGIGRSGRRLVMVIEKRDGHVRGVREDGSSASF
                     PQERIGRVYSPVYRLREQEIEQAFDEIHERGRELTLPEPRLRDVHAEETDALRILDDS
                     IENLLPPHITGPDRLRCTELLWELHETAEDYERATRRIESLREEVWLPFEQRAKVLSV
                     FGYLDYEAEKVTERGRWLADLHIDRPLLVGEALESGLFNALDFKQLAGIMAALTADEE
                     RDYGDLELEDNIVTSLSRFEDIGFKVSAEEWKYGIEPAPELNFSAAGATVLWAGGASW
                     ADIVRQTRAEEGDLFRMFSRTGEALLQVAGLYRTHPQAAQMAATAAEIVLREPIR"
BASE COUNT          581 a          868 c          827 g          622 t
ORIGIN      
        1 cagcatcatc atgctgaatc cggcggcgtc tggcggtgtc acgcgatctt cattcggaac
       61 gaattccagc gtcacagcgc gaccaccggc tgcgagcgcg ctgtaaactt tgcgcaggag
      121 cgtctcacaa gtcggcggat cgaagtgatg cagaaagttt gtgagcagga ccaggtcgta
      181 accggagcca aactcaacat caaaggcgct gccttcgatc gtgttgtagc gatcggaaac
      241 gcccgccttc tgcgcattct cctttgcgac ctcgagcaca gccttccagt cgatcgccgt
      301 gatctcagct tgcgggttgt tggtggcaaa agcgatgccg aatagaccat gaccggcggc
      361 gatgtcgagt actttcaact tgcgatcggc gtttggatcg acgagcttcg caatcaactg
      421 cgccggcaag gccatcatcg cgcccatgcc gcgtgcaaac ttcacccaga cggggttctc
      481 cggtccaatg gtgccttggt cgtccatcac ggtgccgcct ttgcgcacgg cttccgcgat
      541 gtgtttggcg ctatcggtca gcagcggcga acacatgaat tcgaccgtgc cgcccagata
      601 cgcaggcgag cgcttatcga gaaatgtggc tgaatcaagt gtcaacgcgt agttgtcacc
      661 ttctttggtg agcattccca tgatggtgag gaagtcgcag agaatgcgca tgcctttctc
      721 ggacgtctgg cagcgttgcg cgatttgtgc ggcggttgta ttgccttcgc cgatggcggt
      781 gaacacttcc agctcgatcg cggctttcaa agcttcggtt cgttgatgtg cgttgatggt
      841 ttggaaaaac agttgtggtg atggttgctg ggcagcggaa gtcgacattc aagatcctct
      901 cttcttttag ttatctgttt ttgtctgtgg tccgctcatc ctaccgaatt ggctctctca
      961 atactatctc agccgccgta gctgccatct gcgcagcctg tggatgagtc cgatacaagc
     1021 ctgcaacttg aagtagcgct tcaccagtcc gcgaaaacat gcgaaacaga tcgccttctt
     1081 ccgcgcgcgt ctgtcgaaca atgtcggccc acgaagcacc gccggcccaa aggactgtcg
     1141 cacccgcggc ggaaaaattc agctccggcg ccggctcgat gccgtatttc cattcctcag
     1201 ccgagacttt gaagccgata tcttcgaagc gcgagagcga cgtgacgata ttgtcttcga
     1261 gttcgagatc tccgtagtcg cgttcttcgt cggcggtgag tgcggccatg atgccggcaa
     1321 gctgtttgaa atcgagagca ttgaacagac cgctttcgag agcttcgccg acgagcagcg
     1381 gtcgatcgat gtgcaggtct gcgagccagc ggccgcgttc ggttactttt tccgcttcgt
     1441 agtcgagata tccaaatacg gaaagcactt tcgcgcgttg ctcgaaaggg agccagacct
     1501 cctcgcgtaa cgattcaatc cggcgcgtgg cgcgttcgta gtcttccgcc gtctcgtgca
     1561 gttcccacag aagctcagta cagcgcaagc ggtctggacc agtaatgtga ggcggcaaca
     1621 ggttctcaat gctgtcatcg agaatcctca gtgcatcagt ctcttcggcg tgcacgtcac
     1681 gcagtcgcgg ctcgggcaac gtcagctcgc gaccgcgttc atgaatctcg tcaaacgctt
     1741 gttcgatctc ctgttcgcgc agccggtaaa cgggcgaata cacgcgcccg attcgttcct
     1801 gcggaaaact cgcgctgctt ccgtcttcgc gaacgccgcg aacgtgtccg tcgcgcttct
     1861 caatcaccat cacgaggcgc cggccgctgc gtccgatgcc aaccacgcgg cctggtttca
     1921 ccacctcgtt tagccagcgg gaaaaaactt ccgcacgcgt ttgcggtttt gcctcctgca
     1981 accgcgctcg cgcaccgatt agtcgttcca gacccagcac tgtggagacc ggaagatcgt
     2041 agccggcgtc gtggagccgc cgttgaatcg tttccgcgct ctgttcgcgg cttttttcga
     2101 gttgactgag ctggcgaact gagtcgcgat gggcaaagct gcgttccgca atctcgcgaa
     2161 cgtgcgcaaa cgttccgtaa gcatcaagca agttgagcag cgtcgtgtaa gtagcgcgga
     2221 attgactgac gagtggatcg ggtggagctt tgagtaattg cgcgatgcgc aacggatcct
     2281 ggtgcaaacc gggcgcagca acaacaaacc caacattgtc acgcccgcga cgaccagcgc
     2341 gtccggtcat ctgttgtagt tcggaagctg acaatggacg ccagccgctc gcggtgcgcg
     2401 cgtctgagcc ggtcaacaca acactgcgag ccggaaaatc aacgcccgct gcaactgttg
     2461 ccgtcgcaaa gatcgcgtcg agcagcccgg cgctcatcaa gcgttcaata acaagtttcc
     2521 acgacgggat gtgtccggcg tgatgcgaag cgacgccacc gcgaatgatc gtgtcccaat
     2581 gccgatggcc gcgcacttcc ggatgctgct ccacaaacga acgcatgaac tcgcgccgcg
     2641 cttcgcgtcg aggatcgctg cccgttggac gcgcaagcgc cgcttccgac gccgccttgt
     2701 cgcaacgacg gcgcgtcggc atgaacacaa tcgcaggcag taggttgtaa ccgtccaacg
     2761 ccgacagcaa cgtcgccggc ggcatctcag gcattcgcag tttcatgtat tagctgtgcg
     2821 atttgtggat taacccttcc gccctcctcg atcaaaggca acagccgccg atccggaagc
     2881 aacaccgccg aacgcagc
//
LOCUS       DASTEY010000089         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1303825, whole genome shotgun sequence.
ACCESSION   DASTEY010000089 DASTEY010000000
VERSION     DASTEY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303825"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..646
                     /gene="atpH"
                     /locus_tag="VFO72_01530"
     CDS             104..646
                     /gene="atpH"
                     /locus_tag="VFO72_01530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET9477994.1"
                     /translation="MSLQTIARRYASALADVVIERGEEREVLKEVEFWASMIDSNPQL
                     KEVFANPTVAYDRKRSVLEELISRTRVRETTASFLRVLLRNQRLAQLPEVAERFSRIL
                     DERGGVVAAEITTARPIPEELKKTLQDTLASATGRTVRLTFATDEAIIGGLVARIGST
                     IFDGSVENHLERLAEGMAGQ"
     gene            671..2257
                     /gene="atpA"
                     /locus_tag="VFO72_01535"
     CDS             671..2257
                     /gene="atpA"
                     /locus_tag="VFO72_01535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9477995.1"
                     /translation="MEPIRASEINDIIRKQIENFDTGVTVTEVGTVIKIGDGIAEIHG
                     LEKVMAGELLEFPHDVRGLALNLEEDKVGAVLFGEFQTIREGDLVKRTGRIMQVPVGE
                     ALIGRVVDVLGNPIDDGGPILTEQFNPVERIAPGVVDRQPVKEPLQTGLKAIDAMVPI
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGKDVICIYVAIGQKASTVAQVVKTLQDFGA
                     MDYTIVIKATASDPAAMQFLAPYSGAAMGEYFRDNGKHALTIYDDLSKQAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLSDAKGGGSLTSLPIIETQAGDISAYIPTN
                     VISITDGQIFLEADLFNSGFRPAINVGNSVSRVGGSAQIKAMKQVAGTLRLDLAQFRE
                     LQAFAQFGSDLDKATQAQLARGQRLTEILKQPQYQPMDVEKQVLVIWAATNGFTDDIP
                     VEDVRRFETGLLQFVESSHPGVLAAIAEKKALTDEIKADLKQVLEDFKDDWNKVAAGT
                     TDFAAAPVTQASAAAAPQPA"
     gene            2268..3155
                     /gene="atpG"
                     /locus_tag="VFO72_01540"
     CDS             2268..3155
                     /gene="atpG"
                     /locus_tag="VFO72_01540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET9477996.1"
                     /translation="MPNLLDIRRRIKSVKNTQQITKAMKMVSAAKLKRAQDRVITARP
                     FANKMTEVLGELAKRTDEDFHHPLLDLRGDQRYLLVLITADKGLCGAFNTNLTKAAQA
                     FIRENPDKAIELLAIGRKGRDFFRNRGAVIAAEYIGLTGKGRVEFSEALEVARSIIRL
                     YTEDTGIDKVFLVYNEFKSVLSQRVVLEQLLPVARAKAPEPETKGQQAVTLVDYIYEQ
                     PPDEMFSRLLPRLVETQIFRALLESVASEHGARMTAMDSASKNASELIDTLTLNMNRV
                     RQAAITNEIIEVVSGAAAL"
     gene            3258..3722
                     /locus_tag="VFO72_01545"
     CDS             3258..3722
                     /locus_tag="VFO72_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9477997.1"
                     /translation="MARFLSRYLPLAAWLLFISFASSDSFNAGNTSRIIGPLVRWLFP
                     DTTPETLAVVHFITRKAAHLTEYAILAYLAARAFRTSPRPALANRWFLASLALVVAYA
                     LLDEYHQSFVASRTGSIYDSVIDIAGGLAALLIIRHRRKRARGFKKTPRLSV"
     gene            3763..>4199
                     /locus_tag="VFO72_01550"
     CDS             3763..>4199
                     /locus_tag="VFO72_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9477998.1"
                     /translation="MGILDRLSPSSQFIEVIEWLDDSGNTLIYRFPVRDQEIKNGAQL
                     IVRESQAAVFVHEGQIADQFGPGRYTIDGGNTPILSKLGAWKYGFNSPFKSEVYFVST
                     KQFQDLKWGTPNPVMMRDTDFGMVRLRAFGIYSIRVADPQAFI"
BASE COUNT          990 a         1182 c         1153 g          874 t
ORIGIN      
        1 aaagtatcga cctcgcgcgg cagacagttc gtaatcaaat gcgacctgaa gacgatagac
       61 atttgattga gaacagcata ggtgaattga ggagggcccg agtttgagct tgcagacgat
      121 cgcgcgccga tacgcgtcag ccctcgccga tgtggtcatc gaacgcggtg aagagagaga
      181 agtcctaaaa gaggtcgagt tttgggcttc aatgatcgac agcaatccgc aattaaaaga
      241 agtcttcgcc aatcctaccg tagcctacga tcgcaaacgc agcgttctcg aggagttgat
      301 ctcgcgcact cgcgtcaggg aaacaaccgc cagcttcctg cgcgtactat taagaaatca
      361 gcggctcgcc cagttgcctg aagttgcaga gcggttcagt cggatcctgg acgagcgcgg
      421 cggcgtcgtg gcagcggaga tcacaacagc gagaccgatt ccggaagagt tgaagaaaac
      481 attgcaggac accctcgcgt cggcaactgg acgaacagtg cgcctcacct tcgcaactga
      541 cgaagcgatc atcggcggcc tcgtggcacg catcggctcc acgatattcg atggttccgt
      601 agagaaccat ctggaacgat tggcggaagg catggccgga caataacacc ggcgcagtat
      661 cagaggacat atggaaccaa ttagagccag cgaaatcaac gatattatcc gcaaacagat
      721 tgaaaacttt gacaccggcg tgacggtcac cgaggttgga actgttatta agatcggtga
      781 cggcatcgcc gagattcacg gtctcgagaa agtcatggcc ggcgaactgc tcgagtttcc
      841 gcatgacgtc cgcggactgg cgctcaacct cgaagaagac aaagttggcg cggtgttgtt
      901 cggtgagttt caaaccatcc gcgaaggcga cctcgtcaag cgaacgggcc gcatcatgca
      961 ggtgcctgtt ggcgaggcgt tgatcgggcg tgttgttgat gtgcttggca accccatcga
     1021 cgacggtggt ccaatcctca ctgagcaatt caacccagtc gaacgcattg ctcccggcgt
     1081 ggtcgacaga caaccggtca aagagccact gcaaaccggt ctcaaagcca tcgacgcgat
     1141 ggtgcccatc ggtcgcggtc agcgcgaact gattatcggc gatcgacaaa ccggtaagac
     1201 cgcggtcgcc gtcgacacca tcatcaatca aaaagggaag gacgtaatct gtatttatgt
     1261 cgcgattggt caaaaagcct cgaccgtagc ccaggtcgtt aaaacgcttc aggacttcgg
     1321 tgcgatggac tacaccatcg taattaaggc cacagcgtca gacccagcgg caatgcaatt
     1381 cctggcgcca tactccggcg ctgcgatggg cgagtacttc cgcgacaacg gcaagcacgc
     1441 gcttacgatt tacgacgacc tttcgaaaca ggcagccgct tatcgcgaga tatcgttgct
     1501 gctccggcgt ccgccgggcc gcgaagctta tccaggcgac gtcttctatc ttcactcgcg
     1561 cctcctggaa cgcgcggcaa agctatcaga cgcgaagggc ggcggttcgc ttaccagttt
     1621 gccgatcatc gaaacacaag cgggtgacat ctccgcttac attcccacga atgtgatctc
     1681 gattacggac ggccagatct ttctcgaagc cgaccttttc aacagtggct tccgtcccgc
     1741 gattaacgta ggcaacagcg tatcgcgcgt aggcggttcg gctcagatca aagccatgaa
     1801 gcaggttgcc ggaacgttgc gtctcgacct cgcgcagttc cgcgagttgc aagcgtttgc
     1861 gcagttcggt tccgacctcg acaaggctac gcaagcgcag ttggcgcgtg gtcaacggct
     1921 cactgaaatt ctgaaacagc cgcaatacca accgatggac gttgaaaagc aggtgctggt
     1981 gatttgggcc gccacgaatg gtttcactga tgatattccc gtcgaagatg tgcggcggtt
     2041 cgaaacgggt ttgctgcaat ttgtcgaaag ttcacatccg ggcgtgctcg cagccatcgc
     2101 ggaaaagaaa gctttgactg atgagatcaa ggccgacctg aaacaagtgc tcgaagattt
     2161 caaagacgac tggaacaagg tcgcggcggg cacgacagac ttcgccgcgg cgcctgtgac
     2221 gcaagcttcg gccgcagccg cgccgcagcc agcgtaaaca ataagcaatg cctaatcttc
     2281 tcgatatccg ccggcgcatt aagtcggtta agaatacgca gcagatcacg aaagccatga
     2341 agatggtttc tgccgcgaag cttaaacgcg cgcaggatcg cgttatcacc gcccggccgt
     2401 ttgcaaacaa gatgacggaa gttttaggcg agcttgcgaa gcgaactgac gaagactttc
     2461 atcatccgct gctcgatctc cgcggcgacc aacgttatct gcttgttctg atcactgccg
     2521 acaagggctt gtgcggtgcc ttcaacacaa acttgacgaa agcggcgcag gcgtttatcc
     2581 gggagaaccc cgacaaggcc attgaactcc tcgcgattgg gcggaaaggc cgcgacttct
     2641 ttagaaatcg cggcgcggtc attgccgcgg agtatatcgg tctgacgggc aagggccggg
     2701 tcgagttttc tgaagcactc gaagttgcgc ggtccattat caggctgtac acggaagata
     2761 ccggcatcga taaagttttc cttgtctaca acgagttcaa atccgttctt tcgcaacgcg
     2821 tcgtgttgga acagttgctg ccggtcgcgc gagcgaaagc gccggagccg gagacgaagg
     2881 gccagcaagc cgtcacgctt gtggattaca tctacgaaca accgcccgac gagatgttta
     2941 gtcgacttct gccgcgtttg gttgaaactc aaatattccg cgcgctactt gaatcggtgg
     3001 cttccgaaca cggtgcgcgg atgaccgcga tggattccgc ttcgaagaac gctagcgagc
     3061 tgatcgatac tctgacgctc aacatgaatc gcgtcagaca ggccgcgatc accaacgaga
     3121 tcatcgaagt cgtttccggc gccgccgcac tgtagattac ctgacacgat gaacagtatt
     3181 aacaggacta aggaagacaa cgactctcgc tgccttcgtg ttaattctgc aatcctggcg
     3241 acggcttaat tggatctgtg gccagatttc ttagccgtta tcttcctctc gctgcctggc
     3301 tgctgttcat ctccttcgct tcttccgaca gcttcaacgc gggaaacact tcgcgcatta
     3361 tcggaccact cgtacgctgg ttatttccgg acaccacccc cgaaacgctc gcggtggtcc
     3421 atttcatcac ccgaaaagcc gcgcacctca ccgaatatgc gattctcgcg tacctcgcgg
     3481 cgcgtgcgtt ccgcacatcg cctcgtcccg cgcttgcaaa ccgctggttt ctggcgtcgc
     3541 tcgcactcgt cgtggcgtac gcattgctcg acgagtacca ccagagtttc gtcgcgtccc
     3601 gaacaggctc catctatgac agtgtcatcg acatcgccgg cggtctagcg gccctgttga
     3661 taatccgcca tcgtcgcaaa cgcgcgcgcg ggttcaagaa aactccacgt ctcagcgtat
     3721 aatcggccct tgactgaatc cttgcagaat gaggccacaa aaatgggaat acttgatcgt
     3781 ctaagcccga gttcacagtt tatcgaggta attgaatggc tcgatgacag cggtaacacg
     3841 ctgatttatc gatttcccgt ccgagatcag gagatcaaaa acggagcaca gttgattgtg
     3901 cgagagtcgc aggcggctgt cttcgttcac gaaggccaga ttgcggatca gttcggaccg
     3961 ggccgttaca cgatcgacgg cggaaacacg cccatcttgt cgaaactggg cgcatggaag
     4021 tacggtttca actcgccctt caaatccgaa gtctacttcg tcagcaccaa acaatttcag
     4081 gatctcaagt ggggcacacc aaacccggtc atgatgcgcg acactgactt cggcatggtc
     4141 cgtttgcgcg catttggaat ctattcgatt cgcgttgccg atccgcaggc cttcatcaa
//
LOCUS       DASTEY010000090         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1271029, whole genome shotgun sequence.
ACCESSION   DASTEY010000090 DASTEY010000000
VERSION     DASTEY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271029"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="VFO72_01555"
     CDS             complement(<1..1738)
                     /locus_tag="VFO72_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9477999.1"
                     /translation="MMKLKPIFPGLMAALAVLTLALSGKSQQPAPQQAASRFDVTHYR
                     IEAQIIPEQHLLRAGADITFTPLDATRSVVFELNGSLRVESIERDGKPLTGFVQDAVG
                     VGSLGPSVRIDLGQVVPANQPVTLRIRWSGALVSPEGGPLATKRLAYVGNEGSYLMYA
                     SRWFPFHDYAADRATSDITFIVPSGLQVAGTSDDAVAAQPDAKSGAVRYRFVHRKPVL
                     IGNFAAGQYINKNLRYHNYELQLYAMPGSEKRLEAYAELTGRVLEFYTKQYGAPLFGN
                     RLVVVQIDDESLDTYSGPGMVFLSSKMFDTSRPVPEEKLQREIAYQWWGQTVGLKSFD
                     DAWLSQGLAEWSAFAFRETTLTAGGLDAAQREQQERALTFEQTASIARAPSALDDQSA
                     AYQSIVFYKGSMVFRMLRETIGRENFDRLLRTFLDTYRGKNASIDEFEKLATQVSNEN
                     LRYFFAQWIEGTGVPEFTVEYQIIRTRAGKFRTRGTVKQNLEALRMPVELLLRAEGDN
                     QQTTVRIEGRSEDFDFESNGQPIEVIVDPNNKILRMSDELRVSIIARRGIEQMKEGIY
                     AEAQQQFEAALKL"
     gene            2100..3356
                     /locus_tag="VFO72_01560"
     CDS             2100..3356
                     /locus_tag="VFO72_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9478000.1"
                     /translation="MKNGLRVITVEDHTAPVIAISVTYNVGSRNERKGRTGFAHLFEH
                     MMFKGSQNVGSGEHFLLVFNNGGTMNGTTNEDRTNYFEALPANQLDLALFLEADRMRS
                     LEITKENLENQRHAVQEERRLGIDNQPYGKSNELQQELLYDNFAYKHSVIGSMEDLNA
                     ATVDDVAEFFKMYYAPNNAVLVLVGDFKTADALAKIRTHFESIPRQPAPPAVDMAEPQ
                     QKAERRATVDDQLARVPRVDLAFKAVPGNTADFYALQVLSAALQSGQSSRLYQKLVRE
                     KEMVTGVGGFMNEKRGVGAFYTTATLRPNIKTEDVEAAIYEEIERLRKEPIADWELQK
                     AKNTTRRNFINGLQSSLSRAITIGQYTVYYNDPNLINQRLDKVAAVTKQDVQRVANKY
                     LIDINRTVVITMPKAKAKTATGASGQ"
     gene            3371..>3711
                     /locus_tag="VFO72_01565"
     CDS             3371..>3711
                     /locus_tag="VFO72_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478001.1"
                     /translation="MRMNKFRSNIVAALMLVALVSSLAFGQSGPAPAQQQQTTKGAVI
                     KGKAPVNKELLKVKLPRAEEAKLTNGLQVLLLRNPKVPTFNMQMVVLSGGLSDKPDYR
                     GLASFTASLLR"
BASE COUNT          836 a         1049 c         1022 g          804 t
ORIGIN      
        1 cgagcttcag cgcggcttca aattgctgct gcgcctccgc atatatacct tccttcatct
       61 gctcgatgcc acgccgcgca atgatcgaca cgcgtagttc gtctgacatg cgcagtatct
      121 tgttgttcgg atcgacgatg acctcaatcg gctgtccgtt cgattcgaag tcgaaatcct
      181 cgcttctgcc ttcgattcgc acagtcgttt gttgattgtc gccttcggcg cgcaacagta
      241 actcgaccgg cattcgcagt gcttcgaggt tctgcttcac cgtgccacgc gtgcgaaact
      301 tgccggcacg cgtgcgaatg atctggtact cgacggtgaa ctccggcacg ccggtgcctt
      361 cgatccactg cgcaaagaag taacgcaggt tttcgttgga gacttgcgtc gccagttttt
      421 caaactcgtc tatcgatgca ttcttaccgc gataggtatc gaggaacgtg cgcagcaagc
      481 gatcgaaatt ctcgcgtcca attgtctcgc gcaacatgcg gaaaaccatc gaacctttgt
      541 agaacacgat cgactggtaa gcagcggact gatcgtcgag tgcactggga gcgcgcgcga
      601 ttgatgctgt ttgctcgaac gtcaatgctc gttcctgttg ttcgcgttga gcagcatcga
      661 gacctccggc cgtgagagtt gtttcgcgaa acgcgaaggc gctccattcg gcgagtccct
      721 gcgaaagcca cgcgtcgtcg aatgacttga gaccgacggt ctgtccccac cattgatacg
      781 caatctcgcg ctggagtttc tcttcgggta ccgggcggct ggtatcgaac atcttgctcg
      841 acaaaaagac catgccgggg ccggaatagg tatctaaact ttcatcgtcg atctgcacga
      901 ccacgaggcg attgccgaaa agcggcgcgc cgtattgctt ggtgtagaat tcgagcaccc
      961 gaccggtcag ttccgcgtat gcttcaagcc gcttctcgct gcccggcatt gcgtacaact
     1021 gaagttcgta gttgtggtag cgcaggtttt tgttgatgta ctgtccggcg gcaaagtttc
     1081 cgattagcac gggcttgcga tgcacgaacc ggtaacggac cgcgcccgat ttcgcatccg
     1141 gctgtgcggc gaccgcatca tcgctggtgc ccgcaacctg caatcctgac ggaacgataa
     1201 atgtgatatc ggaggtcgcg cggtctgcgg cgtagtcgtg aaacggaaac cagcgcgagg
     1261 catacatcag atacgaacct tcgttgccca cgtaagccaa acgtttcgtt gccagtggtc
     1321 cgccttccgg agaaacgagt gcgccactcc agcgaatgcg gagcgtcacc ggttggtttg
     1381 ccggaactac ctggcccagg tcgatgcgca cgcttggtcc aagcgaaccg acgcccaccg
     1441 cgtcctggac gaagccggtc aatggcttgc cgtccctctc aatgctttca actctcagtg
     1501 aaccgttaag ctcgaaaacc actgaacgcg tggcgtccaa cggtgtaaac gtgatgtcag
     1561 cgccggcgcg taacaggtgc tgttcgggga tgatctgggc ttcgatacgg tagtgcgtta
     1621 cgtcgaaacg actggcggcc tgctgaggcg caggctgctg cgactttcct gagagggcga
     1681 gcgtcaggac ggccagagcc gccatgaggc cagggaagat gggctttaat ttcatcatgg
     1741 ctttcgtttt tgcgaacgat tatacagccg ttccaagtgc caaaaggaat ccgctgcgag
     1801 ggttttggat ggcgcgaagg gtcggtcggt tggctaaaac ctgccacgat tccccagatt
     1861 accagtaatg tgtggattgc ctttaccttc gcttgacact gcttttctca cagcatacag
     1921 ttttgcttct gcgttgttca tccttgctcg attgagagca ctatccacgt tacacaagga
     1981 gttgatgtat gagacgcctc tctaagcttt tactacctgt gtttgtctgc gcgctcgctg
     2041 ttctccacgg gcaagctcag agcgcccgca gcgtgcgggt tgatttcaaa gaaaccactt
     2101 tgaagaacgg cctccgcgtg atcaccgtcg aagatcacac tgcgcccgtg atcgccattt
     2161 cggtgaccta taacgtcgga tcgcgcaacg aacgcaaggg ccgaaccggt tttgcccacc
     2221 tcttcgaaca catgatgttc aaaggctccc aaaatgtcgg ttccggcgaa cacttcctgc
     2281 tggtcttcaa caacggcggc accatgaacg gcactaccaa cgaagaccgc accaattact
     2341 tcgaggccct gccggccaac cagctcgatc tggctctatt tctcgaagcc gatcgcatga
     2401 ggagcctgga gattactaaa gagaacctcg aaaaccaacg ccacgccgtc caggaagagc
     2461 gtcgcttagg catcgataac cagccctacg gcaagagcaa cgaactccag caggagttgc
     2521 tctacgacaa cttcgcttac aagcattcgg tcatcggctc gatggaagat ctgaacgccg
     2581 cgactgtcga cgacgtcgct gagttcttca agatgtacta cgcgccgaac aacgccgtgc
     2641 tcgtgctggt tggtgacttc aaaactgctg acgcgctggc gaagattcgc acgcatttcg
     2701 aaagcattcc gcgccaacct gctcctccgg cggtcgacat ggctgagcct cagcagaagg
     2761 cggaacggcg cgccactgtc gacgatcagc tggcgcgcgt gccgcgcgtc gatctggcgt
     2821 tcaaagcggt accgggaaac actgccgact tttacgcctt gcaggtgctc tcagcagctt
     2881 tgcagagcgg ccagagttca aggctttatc aaaagctggt gcgtgagaag gagatggtta
     2941 ccggcgtcgg cggcttcatg aatgagaagc gcggcgtagg cgccttctac accactgcca
     3001 ctctgcggcc aaacatcaaa actgaagacg tcgaagcagc catctacgaa gagatcgagc
     3061 gcctgaggaa agagccgatc gctgattggg agctgcaaaa ggccaagaac acaacccgcc
     3121 gcaacttcat caacggtctt caaagctcgc ttagtcgagc cattacgatc ggccagtaca
     3181 cggtttatta caacgatccc aacttgataa atcagcgact ggataaagtt gctgccgtga
     3241 cgaaacaaga cgtgcagcgt gtggccaaca agtatttgat cgacattaat cgcacagtgg
     3301 tgatcacgat gcccaaggca aaggccaaga ccgcaaccgg cgcgtcgggg cagtaaagga
     3361 gtcatgagca atgagaatga ataagtttag aagcaacatc gtcgccgcac tgatgcttgt
     3421 agcgctcgtt tcgtcgttgg cttttggcca gagcggtccg gcgccggccc agcaacagca
     3481 aacaacgaag ggcgctgtaa tcaaaggaaa ggcgccggtc aataaggaac ttttgaaagt
     3541 aaaacttccg cgtgccgaag aagccaaact gacaaacggt ctacaagtac tgctgctcag
     3601 gaatcccaag gtgcccacct tcaacatgca aatggtagtg ttgagcggcg gtctttccga
     3661 caaacccgac tatcgtgggc tcgcgagttt taccgcttcg ttgctgcgcg a
//
LOCUS       DASTEY010000091         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_644624, whole genome shotgun sequence.
ACCESSION   DASTEY010000091 DASTEY010000000
VERSION     DASTEY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644624"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2372
                     /locus_tag="VFO72_01570"
     CDS             <1..>2372
                     /locus_tag="VFO72_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478002.1"
                     /translation="PNEERGVFMTTDGGKTWTKTLYIDNQHGVADLEIDPTNPNILYA
                     GMWSFERKPWTHRSGSEKGGVYKSIDGGRTWNKLTNGLPKLIGRIGIRVAPSNPNVVY
                     AIMEAKDGTLYRSDDRGETFKMVSKQDSIVSRGFYYTTVRVDPTNENRVYAVASTLFV
                     SVDGGKTFRSITSRSHIDFHALWIDPKNPKRMWHGQDGGIGVTYDAGESWEAVYNIPL
                     GQFYQIHADNRQPFYYVMGGLQDNGSWTGPSRTREPAGIMNDDWRMVSFGDGFYVINH
                     PDNPDEYLTESQGGNILWSDMANRELRGVNPWGRGSGGGPAEGRKYRFNWNSPIVFSP
                     HEKTTVYFAGNVVFKTPDFGKTWQQISPDLTTNDPAKLKDAGGPIAIENSTAEYHSTI
                     ISIAESPAQKDQIWVGTDDGNLQLTTDGGKNWSNLIKNVPGVAANSPVSHVEPSRTNA
                     RTAYVSFDRHMFDDFRPYIFKTTDEGKNWTSVSGNLPAKAYVQVVREDPKNTNLLYAG
                     TELGLFASYNGGKDWLPLNLKNLPNVAVHDIVVHPRENDLILATHGRSIWIFDDATVL
                     QQMSQQILNSNAHLFSIRPALRYTTRFTRYGIGDKQFTGPNPPAGALITYYLKEKLDD
                     KATLKVQVFDRDGKLVQDIERPSREKGLNRIAWNLRLGGPEVRRPPTEEQIAFGGGPR
                     GPQVLPGIYTVKLTVNDKVFEERVEVKLDPSIKTSVAELQETLDMQVKLRDMQSTMNM
                     SLRFMDSLKEQLSQAQTTMKKLNKEPDKDMMKALEDYIKEVDKLQDRLAAR"
BASE COUNT          602 a          648 c          655 g          467 t
ORIGIN      
        1 ccaaacgaag agcgcggcgt gtttatgacc acggacggcg gtaagacctg gaccaaaaca
       61 ctctacatcg ataatcaaca cggcgtcgcg gatctcgaga tcgatcccac caatccaaac
      121 attctttacg ccggcatgtg gagctttgag cgcaagccct ggacgcatcg cagcggcagc
      181 gagaaaggcg gagtctacaa gtcgatcgac ggcggccgca cctggaacaa actcactaac
      241 ggcttgccga aattgatcgg gcgtatcggc attcgcgtgg cgccgagtaa tccaaacgtc
      301 gtctacgcaa tcatggaagc caaagacgga acgctttatc gctcagacga tcgcggggaa
      361 actttcaaaa tggtttcgaa gcaggattcg attgtctcgc gtggttttta ttacacgacc
      421 gttcgtgtcg atcctacaaa tgagaaccgt gtctatgcgg tagcctcaac cttgttcgtc
      481 tcagttgacg gtggcaagac atttcgttca ataacgagcc gcagtcacat cgactttcac
      541 gcactctgga tcgatccaaa gaatcccaaa cgcatgtggc acggacagga cggcggcatt
      601 ggcgtgactt acgatgccgg cgaatcctgg gaagcggttt acaacattcc gctgggccag
      661 ttctatcaga ttcacgcaga caaccggcag ccgttctatt acgtgatggg cggactacag
      721 gacaacggtt cctggaccgg cccgagccgc acacgcgagc cggccggcat catgaacgac
      781 gactggcgca tggtcagctt cggcgacggc ttttacgtga tcaatcatcc ggataatccc
      841 gatgaatatc tcaccgaatc gcagggcgga aacattctct ggtccgacat ggctaatcgc
      901 gaactgcgag gcgttaatcc gtggggtcgc ggcagtggcg gtggtccagc ggagggacgg
      961 aagtatcgct tcaactggaa ctcgccgatc gtgttttcac cacacgagaa gacaactgtg
     1021 tattttgcgg gaaacgtagt cttcaagacg cccgattttg gcaagacgtg gcaacagatc
     1081 agtcccgatc tgactacaaa cgatccagcg aagttgaaag acgccggcgg tccgattgct
     1141 attgaaaact cgactgccga gtatcactcg acgatcatca gtatcgccga atcgccagcg
     1201 cagaaagatc agatctgggt gggcactgac gatggcaacc tgcaactcac tacggatggc
     1261 ggcaagaact ggtccaactt aattaagaac gttcctggcg ttgccgcaaa ctctcctgtt
     1321 tcacacgtcg aaccttcgcg cacgaacgcg cgcaccgcgt acgtctcatt cgatcgtcac
     1381 atgtttgacg acttccggcc gtacatcttc aaaaccaccg acgaaggaaa gaactggacg
     1441 agcgtctcag gcaatctgcc ggcgaaggcg tatgttcagg tcgttcgcga ggatccaaag
     1501 aacacgaacc tgctttacgc aggcacggag ctcggtttgt ttgcttcgta taacggcggc
     1561 aaagactggc tgcctctgaa tctaaagaac ctgccgaacg tcgcagtcca cgatatcgtc
     1621 gtgcatccgc gcgagaacga tctcatcctc gccacgcacg gccgcagcat ctggatcttc
     1681 gacgacgcca ccgtgctcca gcagatgtcg cagcagatcc tgaacagcaa cgcgcacctg
     1741 ttttcgatac gaccggcact gcgatacacg actcgcttta cgcgttacgg tattggcgac
     1801 aaacagttca ccggacctaa tccgccggcg ggagcgttga taacctacta cctgaaggaa
     1861 aaactcgacg ataaggctac attgaaagtt caggtcttcg atcgtgacgg caagctggtg
     1921 caggacatcg agcgcccgtc acgcgagaag ggtttgaatc gcatcgcgtg gaacttgagg
     1981 ctcgggggtc ccgaagtccg gcgcccgcca acggaggaac agatcgcgtt tggcggcggg
     2041 ccgcgtggtc cacaagtgct gcccgggatc tacacggtga aactcacggt caacgacaag
     2101 gtcttcgaag aacgagttga ggtaaaactt gatccgagta tcaagacctc ggttgccgaa
     2161 cttcaggaga cgctcgacat gcaggtcaag ctgcgcgaca tgcagtcgac gatgaatatg
     2221 tcgttgcgat tcatggatag tctgaaagag caactcagtc aagctcaaac cacgatgaag
     2281 aagctgaaca aagagcctga caaagatatg atgaaggcgc tggaagatta cataaaggaa
     2341 gtcgataagt tgcaggatcg actggcggcg cg
//
LOCUS       DASTEY010000092         4950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1040289, whole genome shotgun sequence.
ACCESSION   DASTEY010000092 DASTEY010000000
VERSION     DASTEY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040289"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFO72_01575"
     CDS             <1..546
                     /locus_tag="VFO72_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478003.1"
                     /translation="SDDLRRAIGILKTRPEADMGDGFLVSRRTFYQQRWIRGGGWYPD
                     RQLRLFNRTKGSWKQRHIHESVSMIPGARIGQLGGDLLHYTSPNAAHHHRMIGERYAP
                     LAAQQMFEEGRRTSIFGVATAGPSAFLRSFIFKGGFRDGFAGFAIASFAAHHAFLKHL
                     MLWEKQATDHTDKHREKNARP"
     gene            533..1402
                     /locus_tag="VFO72_01580"
     CDS             533..1402
                     /locus_tag="VFO72_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET9478004.1"
                     /translation="MPDLSLVPPAFGYVLAAVFGAIIGSFLNVVIHRVPLEESIVFPN
                     SRCPSCGAVIAFYDNIPVVSYVLLRARCRGCRQPISFRYPAVELLTAALFVAVAWHDG
                     LSVVLPFDLIFTSALLALIFIDAEHMILPNVITYPGIVFAVVARIAIPYLSGTPHFDD
                     LPSLSQGAFAGMPLWVTSLAGAAIGALIGGGSLWLMGWTWEKLRGIEAMGLGDVKMMF
                     MVGAYLGWRLTILTIFVGVLTGSIIGVVLMARQRERNMQMLLPFGIFLGLGAITALLF
                     GSRWVEWYAGQFR"
     gene            1509..2978
                     /locus_tag="VFO72_01585"
     CDS             1509..2978
                     /locus_tag="VFO72_01585"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478005.1"
                     /translation="MKTRFQSGLTMLTGLFLLAALFAILLYALTPETPAARGPILLAS
                     FIAILLGSLLCILFLLRWLLRPYHQLVGEAQRASAANELLRSQDEAEFVLETFQSVVA
                     QLQEQRKELEQLSAEARERASSAEKFSERIVASLPSGLLAFDGAGLSMVVNTPGQTLL
                     GLDGRALGESYETLLAHHVELAMMVADCLKRGTLYRRSEVESRTEDGRLRRLGATIAP
                     IELPPDRVRPGMAGVSPATRGALCLLTDITEVTELREQVALKNNLESLGEMSAGLAHE
                     FKNAIATLQGYAQLLQSLELNDKAQVAAASLLNEVRNLATMVTAFLNFARPQPLQLEE
                     VDLQELILDCRTELESVFHELNVDLAVDSKGSLIVRADERMLRLALQNLLRNAAEAIP
                     VDKTERSVVVRTAREAHLAVIEVRDTGSGIPAAQLQKIFIPFFTTKPKGHGVGLALTH
                     RIITEHGGTLTAANAVDGGAVFTIKIPSENTHASADRQV"
     gene            complement(2929..4689)
                     /locus_tag="VFO72_01590"
     CDS             complement(2929..4689)
                     /locus_tag="VFO72_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET9478006.1"
                     /translation="MHPSLRTFLDRLTRENEIITIKAEVDPYLELAEIHRRVIAEGGP
                     ALFFERVKGSRYPVVTNMFGTERRIELAFGPKPEAFVREVVHIAESLLPPKPAELWEH
                     RSIALDLLKLGTRKTRRAPVTQVIDKPARLDQLPVLTTWQEDGGPFITLPLVYTENPI
                     TGKHNLGIYRMQVYDAQSAGLHWQIQKGGGFHYHEAEKLNQSLPVTVFLGGPPALILA
                     AIAPLPEDVPELVLASLLAGERLRMTVCPIPGRHRLAAEAEFALVGQAKPHERRPEGP
                     FGDHYGYYSLRHDYPVFHCDAVIHRRDAIYPATVVGKPRQEDFFIGDYLQKLLSPLFP
                     LVMPSVRDLWTYGETGFHSLCAAVVRERYGREALVSGFRILGEGQLSLTKFLILTDAP
                     QDLKNFPRLLEHVLERFRPETDLFVFSNVSMDTLDYTSGKVNEGSKAIMLGLGDAMRT
                     LPREFKEQLPGYVSKAEVFCGGCLVLQGAAFEEDEELAMRVAREQIFADWPLIVLHDD
                     VLAARSSPSFLWSTWTRFEPAADIYAAATNVQRHHLVYKAPIVIDARTKPGFPDELIV
                     REDIAKLVDRRWREYFPKGF"
     gene            complement(4690..>4950)
                     /locus_tag="VFO72_01595"
     CDS             complement(4690..>4950)
                     /locus_tag="VFO72_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478007.1"
                     /translation="SPDLLYPFLSKRNNPARVNAGLLKVLARLGDAETLDLIKPFRES
                     YQPYVAEAAENAYDEIEKRLAEPAAATTRARPRTVEKATDKP"
BASE COUNT         1011 a         1418 c         1401 g         1120 t
ORIGIN      
        1 tcagatgacc tgcgacgagc gatcggaata ctgaaaacgc gtccggaagc ggacatgggt
       61 gacggcttct tggtctcgcg ccgcacgttc tatcaacaac gatggattcg cggtggcggc
      121 tggtatcccg atcggcagtt gcgtttgttc aatcgcacca aaggaagctg gaaacagcgt
      181 cacattcacg agtcagtgag tatgatcccg ggcgcgcgta tcggtcagct tggcggcgac
      241 ctgctgcact acacgtcgcc caatgcagcg caccatcacc gcatgatcgg tgaacgttac
      301 gcgcctctgg cagcgcaaca gatgtttgaa gaagggcgcc gcacttcgat cttcggcgtc
      361 gccacggccg gcccatcggc tttcctgcgc agcttcattt tcaaaggcgg ttttcgagac
      421 ggttttgctg gttttgcgat cgccagcttc gcggcacatc acgcgtttct gaaacatctg
      481 atgctctggg aaaaacaggc caccgatcat acggacaaac accgagagaa aaatgccaga
      541 ccttagtcta gttccgcctg cgtttggtta cgttcttgcc gcagtcttcg gcgccatcat
      601 tggcagcttt ctaaacgtcg tcatccatcg cgtgccgctg gaagagtcga ttgtgtttcc
      661 caactcgcgc tgcccttcgt gcggcgccgt gatcgcgttc tatgacaata ttccggtggt
      721 tagttacgtg ctgctgcgcg cgaggtgtcg cggttgtcga caacccatct cgtttcgcta
      781 tccggccgtc gaacttctga ctgcggcgct cttcgtcgcc gtggcctggc acgatggttt
      841 aagcgtggta ctgcctttcg atctgatttt cacttcagcg ttgctcgcac tcatctttat
      901 cgatgccgaa cacatgattc tgcctaacgt gatcacttac cccggcatcg tcttcgcagt
      961 cgtcgcgcgc atcgcaattc cttatctgag cggcacaccg catttcgatg acttgccgtc
     1021 actatcgcag ggcgctttcg cgggcatgcc cctctgggtt acgtcgttgg cgggagcggc
     1081 gattggcgcg cttatcggtg gcgggtcgct gtggctgatg ggttggacct gggaaaaact
     1141 tcgcggcatc gaggcgatgg ggctcggcga cgtgaagatg atgtttatgg tgggcgcgta
     1201 tctcggttgg cgactcacga ttctcacgat cttcgtcggc gtgctgacgg gctcaatcat
     1261 cggcgtagtg ttgatggcgc ggcagcgtga acgaaacatg cagatgctgc tcccgttcgg
     1321 catctttctc ggactcggcg caatcacggc gctgcttttc ggatcgcgat gggtcgaatg
     1381 gtacgcgggc cagttccggt gattgtagtc attgccgatt gccaaacgcc gattgccgat
     1441 ttgactttcg attacttgcc gcaacggcgc ttgcgtaaca attggcaatt ggcatttggc
     1501 atttggcaat gaaaactcgc ttccaatcgg gcttgaccat gctgacgggc ttgttcctgc
     1561 tggccgcgct gtttgcgatc cttctctacg cactgactcc cgaaacacct gctgcgcgtg
     1621 gtccaatcct gctggcctcg tttattgcca tcctgcttgg cagtctgctc tgcatattgt
     1681 ttctcctgcg ctggctgctg cggccttatc accagctcgt tggcgaagca cagcgcgcgt
     1741 cggcagcaaa tgagttgttg agatcgcagg acgaagcaga gtttgtgttg gagacgttcc
     1801 agtccgtcgt cgcgcagttg caggaacaac gaaaagaact cgaacagctg agcgccgaag
     1861 cgcgcgagcg cgccagttca gcggagaaat tcagcgaacg catcgtcgcg agcctgcctt
     1921 caggtttgct cgctttcgat ggcgccggac tttcgatggt cgtcaacacg cccggacaaa
     1981 cgctactcgg tctcgacggc cgagcgctgg gagagtcgta cgaaacgctg ctcgcccatc
     2041 acgtcgagct ggcgatgatg gttgccgact gtctcaaacg gggaacgctc taccggcgct
     2101 cggaagtcga gtcgcgcact gaagacggcc ggttacggcg tttaggtgcc acgatcgcgc
     2161 cgatcgagtt gccgcccgat cgtgttcgcc ctgggatggc aggcgtctcg cctgcgacac
     2221 gcggcgcgtt atgcctgtta acagacatca ctgaagtcac tgagttgcgc gagcaggtcg
     2281 ctttgaagaa caacctggag agtctgggtg agatgtctgc cgggttggcc catgaattca
     2341 agaacgcgat cgctacgttg cagggttatg cgcagctctt gcagagtctt gagttgaatg
     2401 acaaggcgca ggtcgcggct gcttcgttgc tgaatgaggt gcgtaattta gcgactatgg
     2461 ttacggcgtt tttgaacttc gcgcggccgc agccgttgca actcgaggag gtagatcttc
     2521 aggaactgat tcttgattgc cggactgaac tggagtcagt ctttcacgaa ctgaacgtag
     2581 atcttgccgt agattccaaa ggctcgttga ttgtccgcgc ggacgaacgg atgttgcgac
     2641 tcgctctgca aaacttacta cgtaatgcgg ctgaagcaat tccagttgac aagactgaac
     2701 gctcggtcgt ggtgcgaaca gcgagagaag ctcacttggc ggtgattgaa gtcagagaca
     2761 ccggatccgg cattcctgcc gctcagttgc aaaagatctt cattccattt ttcactacca
     2821 aacccaaagg acacggcgtt ggattagcac tgactcaccg catcatcacc gaacatggtg
     2881 gaacactgac tgctgcgaac gcagtcgacg gcggcgcagt gttcactatc aaaatccctt
     2941 cggaaaatac tcacgccagc gccgatcgac aagtttagca atatcctcgc gcacgataag
     3001 ctcatcggga aaaccgggtt tagtgcgggc gtcgatcacg atcggtgctt tgtaaaccag
     3061 gtggtgtcgt tgcacgttgg tcgcggcagc gtaaatatcc gccgccggct cgaagcgcgt
     3121 ccaggtggac cacagaaacg atggtgacga tcgagcggcg agaacatcat cgtgtagcac
     3181 aatcaacggc caatccgcaa aaatctgctc cctggccact cgcatcgcta actcctcgtc
     3241 ttcttcgaaa gccgctccct gcaacaccaa acacccgccg caaaagactt ccgccttcga
     3301 aacatacccc ggcaactgtt ctttaaactc gcgcggcagc gtccgcatcg catcgcccaa
     3361 accaagcatg atcgccttgc tgccttcgtt tactttgccg ctggtgtaat cgagtgtgtc
     3421 catcgacacg tttgagaaca cgaacagatc agtctccgga cggaagcgtt ccagaacatg
     3481 ctccagcaac cggggaaagt ttttcagatc ctgcggcgcg tcggtaagaa tcagaaactt
     3541 cgtcaacgag agctggcctt cgccgagaat acgaaaaccg gaaaccagcg cctcacggcc
     3601 gtatcgctcg cgcaccactg ccgcacacaa cgaatgaaat cccgtttcgc catacgtcca
     3661 aagatcgcga accgacggca tcaccagtgg gaacagcggc gacaacaact tctgcaagta
     3721 atcgccgatg aagaaatctt cctggcgcgg cttgcccacg accgttgccg gatagatcgc
     3781 gtcgcgacgg tgaatcactg cgtcgcaatg gaagaccgga tagtcgtgac gcaaggagta
     3841 atagccgtag tgatcgccga acggtccttc cggtcttcgt tcgtgaggct tggcctgtcc
     3901 aactaatgcg aattcagctt ccgcggcgag acgatgccgg ccagggatgg gacagaccgt
     3961 catacgcagt ctttcaccgg ctagtagaga cgcgagcact aactccggca cgtcttcggg
     4021 caacggcgcg atcgccgcga gaattaatgc gggtggacca cctaaaaaaa ctgtaaccgg
     4081 caaagactga ttcaacttct cggcttcgtg gtaatgaaac ccgccgcctt tttgaatctg
     4141 ccaatgcagt ccggccgact gcgcatcgta gacctgcatg cgataaatgc cgagattgtg
     4201 ctttccagtg atcgggttct cagtgtagac gagcggcagc gtgatgaatg gtccaccgtc
     4261 ttcctgccac gtcgtcagca ccggcaattg atcgagccgc gcaggcttgt cgatgacctg
     4321 cgtcaccggc gcgcggcgtg ttttccgtgt gcctaatttc aacagatcca gcgcgatcga
     4381 acgatgttcc caaagttccg ccggctttgg cggcaggaga gattccgcaa tgtgcaccac
     4441 ttcgcgcacg aacgcttccg gtttcggacc aaacgccagc tcgatacgac gctcagtgcc
     4501 gaacatgttc gtcaccaccg gataccggct gcccttcaca cgctcaaaaa acaatgccgg
     4561 cccaccttcc gcgatcacgc ggcgatggat ctctgccagc tcgaggtacg ggtcgacctc
     4621 agctttaatg gtgatgattt cgttttctct ggttagtcga tctagaaatg ttcgaaggct
     4681 cgggtgcatt tacggtttgt ccgtggcctt ctcgaccgtt cgcggccgcg cgcgtgtcgt
     4741 cgctgcggcc ggttcggcca gacgcttctc gatctcgtcg tacgcatttt cggcggcttc
     4801 agctacgtaa ggctgatacg actcgcgaaa aggtttgatc agatcgagcg tctccgcgtc
     4861 gccgaggcgc gcgagtactt tcagcaagcc cgcattcacg cgtgctgggt tgtttctttt
     4921 gctcagaaac gggtagagga gatccggact
//
LOCUS       DASTEY010000093         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_628391, whole genome shotgun sequence.
ACCESSION   DASTEY010000093 DASTEY010000000
VERSION     DASTEY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628391"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFO72_01600"
     CDS             <1..339
                     /locus_tag="VFO72_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478008.1"
                     /translation="KNQLVQEYSGSSFPPLHNLTIPEQDARAPEEDWTAGLPIVRRGI
                     QFQDWKEVMDGILLSLEQTENYERERGAEGTRDEWHDRSIGISEAVAKGVGKWMPDEL
                     VALAERQVKK"
     gene            461..1144
                     /locus_tag="VFO72_01605"
     CDS             461..1144
                     /locus_tag="VFO72_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET9478009.1"
                     /translation="MSFLDNLFVDPKYQTTEPRKPEEIQDPEIIISPDTRREKRLPPG
                     QSRTKKWPILDAHGTPDIDLATWSFEVEGLVEHPMKWSLDEFMQLPAARVYSDFHCVT
                     RWSRLDNVWSGVSTREIAKLVGIKPAAKFVLALAYDYGWTTNVPLEYFLKEDSLFAWA
                     HDGQPIAPEHGGPVRLIIPQLYAWKSAKWVKGVRFLEEDQAGFWEQGGYHMRGVPWGP
                     GDGERFRWS"
     gene            1141..2652
                     /locus_tag="VFO72_01610"
     CDS             1141..2652
                     /locus_tag="VFO72_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9478010.1"
                     /translation="MSVVPKDAAAIILLRQNTDPQNPEVYWVKRSDKLAFLGGYHAFP
                     GGQIDATDAEVDVRNSPDAATAAMISGAARELFEELGVLTARGGETLTKGQRESLLDD
                     LESGRMSWPALLKHYELHLDANDFTFVGRWVTPPFNARRFDTWFFLVDCPLKQEPYLT
                     AGELAAGEWITAHEAYQRWVRDQVVAVPPTLHALRTLASGVDASLVERFLSITHAHGE
                     PARRITFRPNYICFPVRTPTRPPATHTCCYLIHTSKELVVVDPGSPYEEEQEALAECV
                     DEMIGEGRRVREIIVTHLHPDHIAGVNALKTHLGGSVPVAAHRNTAEAISDVQVDRLI
                     EDGDILALEGEPQVRLKALHTPGHARGHLCFHDEDRGALLTGDNVVGFGSVLIDPPEG
                     NMRDYLNSLERMRSLPNLSVLFGGHGPAVATPYQKIDEYISHRLERERNILEAVRNGA
                     TDPKQIVAKVYTDVSPKAYSMAERAVLAHLEKLRDDGAVIIDDEGLWLPADRI"
     gene            2661..>3248
                     /locus_tag="VFO72_01615"
     CDS             2661..>3248
                     /locus_tag="VFO72_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478011.1"
                     /translation="MDYPRTLRELKAARYQTRSVKAELRANLIKKLRSGEKLFPGIVG
                     YDDTVIPQLVNAVLSRHNMILLGLRGQAKSRIIRQLTELLDDRIPIIAGSEVNDDPFR
                     PISAYGRQMLGQHGDMTRIDWVDRDARFVEKLATPDVTVADIIGDVDPIKAAKGGHLL
                     SDELTIHYGLLPRANRGIFAINELPDLAGKIQVGLF"
BASE COUNT          739 a          883 c          946 g          680 t
ORIGIN      
        1 aagaaccaac tggtccagga atactccggc agttcttttc cgccactgca caacctgacg
       61 attcccgagc aggacgcacg cgcgcctgag gaagactgga ctgcgggatt gccgatcgtc
      121 cggcgtggaa ttcagtttca ggactggaag gaggtcatgg atggaatcct tctcagtctc
      181 gaacagacgg aaaactacga gcgtgaacga ggtgctgaag gcacgcgcga cgagtggcac
      241 gatcgtagta tcggcatcag cgaagcagtg gcgaagggag ttggcaagtg gatgccggat
      301 gagctggtgg cgctggctga aagacaggtg aagaagtgaa ctttgtactt agtactttgt
      361 tctttgtagt tagaactttg taaggacttt cttcgaggtc gcgacaaaga acagagtact
      421 aagtacaaaa gaacaaagtt caaagaccaa aggtcaaaac atgagttttc tcgacaatct
      481 ttttgtggac ccgaagtatc aaacgaccga accgcgcaag cctgaggaga tccaggatcc
      541 ggagatcatc atcagtcctg acactcgtcg cgaaaagcgc ctgccgccgg gacagtcacg
      601 gacgaagaag tggccgatac tcgatgcgca cggcacacct gacatcgatc tggccacgtg
      661 gtcgtttgaa gttgaaggac tggtcgagca tccgatgaag tggtcactcg acgagtttat
      721 gcaactgccg gccgcgcgtg tttattctga ctttcattgc gtcacgcgct ggtcgcgact
      781 cgataacgtt tggagcggtg tctcaacgcg cgagattgcg aagctggtgg ggatcaaacc
      841 ggccgccaaa ttcgttttgg cattggctta cgactacggt tggaccacaa acgttcctct
      901 cgagtacttc ctcaaagaag attcactttt tgcctgggcc cacgacggac aaccgatcgc
      961 gccggagcac ggcggaccgg tgcgtctgat cattccgcaa ctctacgcgt ggaaatcagc
     1021 gaagtgggtg aagggcgtgc gtttcctcga agaagatcaa gcgggctttt gggaacaagg
     1081 cggttatcac atgcgcggcg tgccatgggg gccgggcgac ggggaaagat tcaggtggtc
     1141 gtgagcgtag ttccgaaaga cgcagccgca atcatccttc tccggcagaa taccgatccg
     1201 cagaacccgg aagtctactg ggttaagcga agcgataaac tcgctttcct cggcggttac
     1261 catgcttttc ccggcggcca aattgatgcc actgatgcgg aggtcgacgt tcgaaactcg
     1321 cccgacgcgg cgaccgcggc aatgatcagc ggcgctgcac gtgaactttt tgaagagttg
     1381 ggtgtgctaa cggctcgcgg cggcgaaacg ctcacgaaag gacaacgcga gtcgttgctc
     1441 gacgacctcg agtcgggacg catgtcgtgg cccgctttgc tcaagcatta cgaactgcat
     1501 cttgacgcga acgacttcac ttttgtcgga cgctgggtga cgccgccttt caatgcccgg
     1561 cgttttgata cgtggttctt cttagtcgac tgtcccctaa agcaggaacc atatttgacc
     1621 gcgggcgagt tggcagcagg cgagtggatt accgcgcacg aggcgtatca gcgttgggtg
     1681 cgcgatcagg tagtagccgt gccaccgacg ttgcacgcgt tgcgaacatt ggcgagcggc
     1741 gtcgacgcga gtctcgtcga acgttttctt tcaattactc acgcgcatgg cgaaccagcg
     1801 cggcgtatca cgtttcgacc taactacatc tgttttcccg tgcgcacgcc cacgcgtccg
     1861 ccggcaacac acacttgctg ttacttgatc cacacgtcga aagagctcgt ggtcgtggat
     1921 ccgggttcgc catacgaaga agagcaagag gcattagccg agtgtgtcga tgaaatgatc
     1981 ggcgaaggca ggcgagtgcg cgaaataatc gtgacgcatc tgcacccgga tcacattgcc
     2041 ggcgtgaacg cgctgaaaac gcatcttggc ggttccgttc ccgtggctgc tcatcgaaac
     2101 actgccgagg cgataagcga tgttcaggtc gatcgtttga tcgaagacgg cgacattctc
     2161 gcgctcgagg gcgagccgca ggttcgactg aaggctttgc acactccagg acatgcgcgt
     2221 ggtcatcttt gttttcatga cgaggatcgc ggcgcgctgt tgactggcga caatgttgtc
     2281 gggttcggtt cagtgctgat cgatccgccg gaaggcaaca tgcgcgacta cttgaactca
     2341 ctcgagcgca tgcgttcgtt gccaaacttg tccgttctgt tcggcggaca cggtcctgcg
     2401 gtcgctacgc cttatcagaa gatcgacgag tacatctcac accgcctcga gcgagagcgg
     2461 aacatcctcg aagcggtcag aaatggcgcg acagatccga aacaaattgt tgcgaaggtt
     2521 tatactgacg tttccccgaa agcctattca atggctgagc gcgcagtgct tgcgcattta
     2581 gagaagttac gtgatgacgg tgccgttatc atagatgacg agggattgtg gctgccggcc
     2641 gacaggattt aaatacaaag atggattacc cacggaccct tcgcgaactc aaagcggcga
     2701 ggtatcagac tcgctcggtc aaagccgaac tgcgcgccaa cttgatcaaa aaattgcgca
     2761 gcggcgagaa actgtttccc gggatcgtgg gctacgacga tactgtcatt ccgcaactcg
     2821 tcaacgcggt tttgtcgcgc cacaacatga tcctgctcgg actgcgcggc caggcgaaga
     2881 gccgcatcat tcgccagtta accgaactgc tggacgaccg cataccaatc atcgccggct
     2941 cggaagtcaa tgacgatcct tttcggccca tctccgctta cggccggcaa atgctcggac
     3001 aacacggcga catgacccgc atcgactggg tcgatcgtga cgctcgcttt gtcgaaaaac
     3061 tcgccactcc tgacgtaacc gtcgctgaca ttatcggcga cgtcgatccg atcaaagccg
     3121 cgaagggcgg ccacctcctg agtgacgaac tgacgatcca ctacggacta ctcccgcgcg
     3181 cgaaccgcgg catcttcgcc atcaatgagt tgccggatct tgccggcaag attcaggtcg
     3241 gactcttc
//
LOCUS       DASTEY010000094         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1171990, whole genome shotgun sequence.
ACCESSION   DASTEY010000094 DASTEY010000000
VERSION     DASTEY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171990"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFO72_01620"
     CDS             <1..829
                     /locus_tag="VFO72_01620"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9478012.1"
                     /translation="YVLTFDPGLFQPRAEVSERLWRSDLATLPLSMTSETTVTLPTNV
                     STTLSSFTAAAKKAFGDDLLSVVLYGSGAEGKLRPTSDVNVILVLASFEQGLADQLRE
                     PLRLAEAAIKLRVMFLLQDELEPASQAFAVKFADIARRRLVLYGTDPFASISVSRDDA
                     ISRLKQTLLNLILRLREAYIARSLREEQLAVVIADAAGPLRSCASTLLELEGHAAASP
                     KQALEQIAVSSPTAAHDVQLLTEARQRHTLPPGAAAPALFHLIDLARTMWARAAQLK"
     gene            840..1742
                     /locus_tag="VFO72_01625"
     CDS             840..1742
                     /locus_tag="VFO72_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HET9478013.1"
                     /translation="MNPFDLPGPEFLLFYVGFSALAIIALVLTRKAAESSAAPKLDLS
                     DPYLIAFLRGAEPETLRVASVSLIDRGLLVATGTQLRTADNASPDAVRRPIEKELLRK
                     FKRADEASSIFEDSRLKATCKPYEQTLKTAELLPNERLERARWSRLLIACGIVGGVGI
                     VKIMVAFSRGRTNVGFLIILIAVAIFLAVKVSFPRLTESGKAMVADLQNLYSGLKDRA
                     LFLKPGGATIEPIMLAAIFGVSALESADFAFTRVLFPRAAAAQSSATCGSSYASACSS
                     CGSSCSSSSCGGGGCGGGCGGCGG"
     gene            1744..>2317
                     /locus_tag="VFO72_01630"
     CDS             1744..>2317
                     /locus_tag="VFO72_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HET9478014.1"
                     /translation="MNQDRFGIGWRPRLAAGILANLDRIDLVEVIADDYFHAAHKERR
                     ALRTLAAQTPVTLHGVSLGLASAVPVERKRLDDMARLCEEVKPEFWSEHLAFVRGGGI
                     EIGHLAAPPRTEATIEGALANLARAKKIVGVAPQVENVATLIDPPGCDRDEAAWLSAI
                     VLNSESDLLLDLHNLHANALNFNFDSHGFLG"
BASE COUNT          445 a          681 c          671 g          520 t
ORIGIN      
        1 gtacgtgcta acgttcgacc cggggttgtt ccaaccccgg gctgaagtta gcgaacgcct
       61 ctggcgttca gatttagcga cactaccctt aagtatgacc agcgaaacca ccgtcacgct
      121 gccgacaaac gtaagtacca cccttagcag cttcactgcc gccgcgaaga aagcatttgg
      181 cgacgatctg ctctccgttg tgctttacgg aagcggtgct gaaggaaaac ttcgtcctac
      241 ttcagacgtc aatgtgattc ttgtgctcgc gagtttcgag cagggtcttg cggatcagtt
      301 gcgagaaccg ctgcgcctgg cagaagcggc catcaaactt cgcgtcatgt ttctcttgca
      361 ggatgagctg gaacccgcgt cgcaagcgtt tgcagtcaag tttgccgaca tcgctcgccg
      421 ccggctggtg ctctacggca ccgatccgtt cgcctcaatt tctgtctcga gagacgacgc
      481 gatctctcgt ttgaaacaga cgttattgaa tctgatactg cgtctgcgtg aagcgtacat
      541 cgcgcgcagt cttcgcgaag agcaactcgc ggtagtcatt gcagacgctg ctggaccact
      601 gcgctcgtgc gcttcgactc tgctcgaact cgaaggacac gctgctgcgt cacccaaaca
      661 agcactcgaa cagatcgccg tgtcttctcc aaccgcagct cacgacgtac aactcctcac
      721 tgaagcacga caacgacaca cgctgccgcc gggtgctgcc gcgcccgcgc tctttcacct
      781 gatcgatctg gcccgcacga tgtgggcacg cgccgctcag ctaaagtgag gcgaccgtca
      841 tgaatccctt cgatcttccc ggaccggagt ttctcctctt ctacgtcggt ttcagtgcgc
      901 tcgcgattat tgcgctggtt ctcacgcgca aagctgccga atcgtcagcc gcgcccaaac
      961 tcgatctgtc cgatccatac ctgattgctt ttttacgtgg cgctgaaccg gaaacgttgc
     1021 gtgtcgcctc cgtttcgtta atcgatcgcg gcttattggt tgcgacgggc acacaactca
     1081 ggaccgcgga caacgcgtcg ccggatgcag tgcgcaggcc gatcgaaaag gagttgctgc
     1141 gaaagttcaa gcgcgcggac gaggcctcat cgatcttcga ggattctcgt ttgaaagcaa
     1201 cctgcaaacc ttacgaacaa acactcaaaa ccgccgaact cctgccgaac gaacggcttg
     1261 agcgggcgcg atggtctcga ctcctgattg cctgtggcat cgtcggcggg gtcgggatcg
     1321 tgaagatcat ggttgcgttc tcacgcggcc gcacgaatgt cgggtttctt atcattctga
     1381 tcgccgtggc gatctttctt gcggttaaag tttcgtttcc gcggttgacg gaaagcggca
     1441 aggcaatggt tgcagacctg caaaatctct attcgggact gaaagatcgc gcgctgtttc
     1501 tgaaaccggg tggcgcgacg atcgagccca ttatgctggc cgcgattttt ggcgtcagcg
     1561 cacttgaaag cgcagacttt gcgttcacac gagtgctctt tccgcgcgcg gcagcagcgc
     1621 aatctagcgc tacgtgcggt tcgtcatacg ctagtgcgtg ttcctcgtgt ggcagttcgt
     1681 gtagtagctc gtcgtgtggc ggtggcggct gtggcggagg ttgtggcggt tgcggaggat
     1741 gagatgaacc aggaccgttt cggcatcggc tggcggcctc gactcgccgc cggcattctc
     1801 gcgaatctcg accgcatcga tctcgtcgag gtcattgccg acgactactt tcatgctgcc
     1861 cataaggagc ggcgcgcgtt gcgaacgctc gcggcgcaaa cgccggttac gcttcacggc
     1921 gtgtcgcttg gattggcatc cgcggttccg gtggaacgca aacgtctgga cgacatggcc
     1981 cggctttgtg aggaagtgaa gccggagttc tggtcggaac atctcgcgtt tgtacgcggc
     2041 ggtgggattg aaatcgggca cctggcggca ccgccacgga ccgaagcaac gattgaaggc
     2101 gcactcgcta acctcgcgcg tgcgaagaag atcgttggcg ttgcgccgca ggtcgaaaac
     2161 gttgcaacgc ttatcgatcc gccgggttgc gatcgtgacg aagccgcgtg gctctccgcg
     2221 attgtcctca actcggaaag cgatctgctt ctcgatctgc acaacctgca cgccaacgcg
     2281 ctgaacttca actttgattc gcacggtttt cttgggc
//
LOCUS       DASTEY010000095         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_513017, whole genome shotgun sequence.
ACCESSION   DASTEY010000095 DASTEY010000000
VERSION     DASTEY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513017"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFO72_01635"
     CDS             <1..512
                     /locus_tag="VFO72_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9478015.1"
                     /translation="KIMQDKAFQNEKIEFIWNTTLEEILGTPEDGVTGVRLRNLQTGE
                     ESVFDCAGVFVAIGHKPNTELFKGQLDMDDVGYLKTSGHSTATNIPGVFACGDVQDSV
                     YRQAVTAAGTGCMSAIDAERYLDHLPVAMPSGDEVTIEGEHITPDHKAIITPDGHMIS
                     NELTADERG"
     gene            complement(552..977)
                     /locus_tag="VFO72_01640"
     CDS             complement(552..977)
                     /locus_tag="VFO72_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9478016.1"
                     /translation="MKRRELDGCPITGALQMLGDKWTMLIVRELLAGPKRTMELLNGL
                     FPISSRTLVGRLRDMEKDRLVERTDFGGNPPHIEYALTERGMLLLPLLESLRQLGLAL
                     KCNSCEDRRERLGAYCEPCPNQPPFTPQRREKDDSIVLL"
     gene            complement(987..2417)
                     /locus_tag="VFO72_01645"
     CDS             complement(987..2417)
                     /locus_tag="VFO72_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9478017.1"
                     /translation="MIKAEKRNPRKGSVLVVDDEEIMREVLETLLTNEGYRVDLAKTG
                     EEGLEAYGRRAFDVVLLDVSMPGIGGLRALEEFLKMDSDAVILMITAYATFDTAIAAW
                     ERGAFGCIRKPFQNEQITATIAAGIKRRRKEEERRTLRRAMSKAVDRGEIIGRSDVMQ
                     EVFRLVEQVAPARSTVLITGESGTGKELIAKAIHEASTRAGRPFVTVNSSNIPSELLE
                     SELFGHTRGAFTGAVAAKKGLFEVADGGSIFLDEIGDIPPETQVRLLRVIQEREFTPL
                     GDTAPRRVDVRIIAATNIDLKEAVRQGTFREDLYYRLAVVPIELPPLRDRIEDILPLA
                     QHFIQKYNEENARNVSEQLAPDVLALLEAYSWPGNVRELENTIERAVVIAPGDEITKE
                     CLRTEISDPKSVVSGHQDGASNAAVSDIGRGINFYEEVRRFEIDLIRRALEQTGGHQS
                     RAARLLGMNATTLNSKIKTYNINLRP"
BASE COUNT          548 a          757 c          760 g          588 t
ORIGIN      
        1 cgaagatcat gcaggacaag gcgttccaaa acgagaagat cgagtttatc tggaacacaa
       61 cgcttgagga gattctcggc acgccggaag atggcgtgac tggcgttcgt cttcgcaatc
      121 tacagacggg tgaggagtca gtctttgatt gcgccggcgt gtttgtggcc atcggtcaca
      181 agccgaacac ggagttgttc aaggggcagt tggacatgga cgacgttggg tatctgaaga
      241 cgtctggtca ctcaacggcg acgaacattc ccggtgtatt tgcgtgtggc gacgtgcagg
      301 attcggttta caggcaggcc gtgacggcag cgggaaccgg ctgcatgtcg gcgatcgacg
      361 cggaacgtta tctggaccac ctgccggttg caatgccgag cggcgatgag gtcacgatcg
      421 aaggcgaaca catcacgccc gatcacaaag cgatcatcac acccgacggc cacatgattt
      481 cgaatgaatt aaccgcagat gaacgcggat aaacgcagat cagatctgcg tcatccgcgt
      541 ttatctgcgg cttataacaa aacgattgag tcgtctttct cgcggcgctg tggagtgaag
      601 ggtggctggt tagggcaggg ttcgcagtag gctccgaggc gttcgcggcg atcctcacac
      661 gagttacatt tcaaagccaa accgagttgc cgaagtgatt cgagtagtgg caagagcaac
      721 atgccacgct ccgtcaatgc gtattcaatg tgcggtgggt tgccgccgaa gtcagtcctt
      781 tcgaccaacc gatccttttc catgtcgcgg agtcgaccca ccaacgtgcg gctgctgatc
      841 gggaaaagac cattcagcag ctccatcgtc cgctttggac cagccagcaa ctctctcacg
      901 atcagcatcg tccacttgtc accgagcatc tgcaacgctc cggtgatcgg gcagccatcg
      961 agttcacgtc gtttcatcgc gtgtggctaa ggtctcagat tgatgttgta ggttttgatt
     1021 ttcgagttca gtgtcgtggc attcattccg agcaggcgcg cagcgcgtga ttgatgcccg
     1081 cccgtctgtt ccaaagctcg ccggattaga tcgatttcaa accgccgcac ctcttcgtaa
     1141 aagtttatcc cgcgcccgat gtcgctgacg gcagcgttgc tggcgccgtc ctggtggccc
     1201 gaaacgactg actttggatc gctgatctca gtgcgcaggc attccttcgt gatctcgtca
     1261 ccaggcgcta tcaccacagc tctttcgatc gtgttctcaa gctcgcgaac gttgccgggc
     1321 cacgagtacg cctcgagtaa agcaagcaca tccggcgcca gttgctccga aacgttgcgc
     1381 gcgttctctt cgttgtactt ctggatgaag tgttgggcca acggcaagat gtcttcgatg
     1441 cgatcgcgca acggcggtaa ctcgatcggg acgaccgcta gccggtaata gagatcctcg
     1501 cgaaaggtgc cttgtcgtac cgcttctttg agatcgatgt tggtagcggc gatgatgcgc
     1561 acgtcgactc gtcgtggcgc ggtgtcgccg agtggggtga actcgcgttc ctgaatcacg
     1621 cgcaagagtc gcacctgtgt ttccggtgga atgtcgccaa tctcatcgag aaagatcgag
     1681 ccaccgtcgg caacttcaaa cagacccttc ttcgcggcaa ccgcacccgt gaacgcaccg
     1741 cgcgtgtgac caaacaactc cgactccagc agctccgaag gaatattcga actgttgacc
     1801 gtcacaaatg ggcgcccagc gcgcgtgctc gcttcgtgaa tcgctttcgc gatcaactct
     1861 ttgcccgtgc cgctctcgcc cgtgatcaag accgtcgatc gcgccggcgc cacttgttcg
     1921 actaaacgaa atacctcctg cattacgtcg gagcgcccga tgatctcgcc gcgatcgact
     1981 gccttactca tcgcgcgccg caacgtacgt cgttcttctt ccttgcggcg tcgcttaatg
     2041 ccggcggcga tggtggcggt tatctgttcg ttctgaaaag gcttgcggat gcaaccaaaa
     2101 gcgccgcgtt cccaggcggc aatggcggtg tcgaaggttg cgtaagcggt gatcatcagg
     2161 attaccgcgt cggagtccat cttcaaaaat tcttcgagcg cgcgcagacc gccgatgcct
     2221 ggcatcgaaa cgtcgagcaa cacaacgtcg aacgcacgac gcccgtaagc ctcgagacct
     2281 tcctcaccgg tcttggcgag atccactcga tagccctcgt tcgtgagcag cgtctcgagc
     2341 acttcgcgca tgatctcttc gtcatcaacg acgaggaccg agccttttct ggggttccgc
     2401 ttttccgctt tgatcatgaa cgcgctagtt tagcaaacgg gccacagatt tacacggatg
     2461 ctatgcacaa ggatcagatg ctgatctgtg ctaatcaata aaaattatcc gtagagatct
     2521 gtgctagtcg ccgacggcct ggaggcgggt gcggacgcgc gcggtcggca agatgatgcg
     2581 gaaggttgtg ccctggcccg gcgtgctgtc gacggttatg cgcccggcgt gttcctggac
     2641 aattccatac gag
//
LOCUS       DASTEY010000096         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1040341, whole genome shotgun sequence.
ACCESSION   DASTEY010000096 DASTEY010000000
VERSION     DASTEY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040341"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..2370
                     /locus_tag="VFO72_01650"
     CDS             106..2370
                     /locus_tag="VFO72_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478018.1"
                     /translation="MSSRSHSENFRRILGRVVRQDFVGRAGELDRIMAQAEPVNSGRG
                     LLLLMEPSVGVSELLRQAYDQIFNQRTEVIPIYFAITRNETTAVSAAIEFLNTFVQQY
                     IAFRRDEPVVSHAPLTLQDLLELAPPADFEWIEQLVDAYNRLRFSNDDKALVRFCLGA
                     PQRIPAGRGRPFVMLDGSQLAEYLNGAVVLGTEILRVFGRGGFSFVLAGLRRQILEAA
                     HDAKCNFELLDTLRLERLDVTEAARLVEHVARRQQVATSEAARDLLVQQFAGSPFFIS
                     IFLQAAREKHTSLLSYLDCERLYVDELFGGHIHRHFADLLEEISPRLDTRHLLIRLLW
                     EAAAGEEQTSSFEVWRKRLHMTATELEDMLHRLHVQEFVNWDETTVEAGAGPQAWKDY
                     LKIRYRLDVLNEPRALVVADMLADSLKRAPHTMARHYKQIANAGLRDLISRFNTQRVP
                     AVLFDYKTFSNKYKGAESETIVSGLEAETDFVKLPQTVHLASCSAFISDMRQICEEDH
                     CLIAHTFEEGSYSDAQQVIWLAADIESKLEVDVDVARVWCERLENIAVRLGFSRFQIW
                     LISNEGFSDEARSLLVRHKAFSSSRQQLGLVASRLGETAPSPVVPAGPPDEFLMVVPM
                     GEDNELIAANTAEQIAKRLTFRPEAINQIKTAIVEACINASEHSFSPDRKIYQRFRVE
                     SDRLVITISSRGIVPANLNGANSGQEATEAAEERRGWGLKLIRTLMDEVEFERVDEGT
                     SLRMTKYLRPQKST"
     gene            2461..2841
                     /locus_tag="VFO72_01655"
     CDS             2461..2841
                     /locus_tag="VFO72_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9478019.1"
                     /translation="MDTPSGKLVRSHCVGTTAVVYASDYLNKLTGEKIERECRRHLNC
                     GCRALVIDFGDTKLVNSIGISILLGIIDIAEKNDARIIFSEVNSQTKQLFEMLGLTRH
                     VALAKDEREALLNISRCETTPIVH"
     gene            2949..>3500
                     /locus_tag="VFO72_01660"
     CDS             2949..>3500
                     /locus_tag="VFO72_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9478020.1"
                     /translation="MHLLLGTLALRRGAVAECTGHGPIGPLKCVAVWGLGDQIFNALE
                     IEDADKQAFLSGEATESLVRNTTSTNGFLNRHRDKLAAEGIELVFPMVVRGEVTGLIL
                     LGGKASGEEFSTDDFETIRAMVRHIGVGIHTHRLLEQVAQRAEENRRLYDELRAIYRD
                     TVRAFAAAIDIKDKYTQGHSERVG"
BASE COUNT          801 a          996 c          982 g          721 t
ORIGIN      
        1 ggcggcttga cgccgcttta gtattgatca agcgcgatcg gcgtcaagcc gccgcactcc
       61 aagagacgct cggttggtaa ggtcatttgg taaggtcata aagagatgag cagtcgcagc
      121 cacagcgaga attttcgccg catcctcggt cgtgtagtcc ggcaggactt cgtcggccgt
      181 gccggcgaac tggaccgcat catggcccag gcagagccgg tcaactcggg ccgtggtctg
      241 ctgctgctga tggaaccctc ggtaggcgtc tccgaactgc tacgccaggc ttacgatcag
      301 atctttaacc agcgcaccga agtcattccc atctactttg caatcacgcg caacgagact
      361 accgccgtca gcgccgcgat cgagtttctc aacacgttcg ttcagcagta catcgctttt
      421 cgccgtgacg aaccggttgt ttctcacgcg ccgttgacac ttcaggatct actcgagctc
      481 gcgcctcccg cagacttcga gtggatcgaa caactggtcg acgcgtacaa ccgcctccgc
      541 ttcagcaacg acgacaaagc cctggtccga ttttgtctcg gagcaccaca acgcatcccc
      601 gcgggacgtg gccgcccatt cgtgatgctg gacggctcgc agctcgccga atatttgaac
      661 ggcgcggtgg tgctcggaac agagatactg cgcgtgttcg gacgtggcgg tttctctttc
      721 gtcctcgccg gattacgccg ccagatcctc gaagcagcgc acgacgcaaa gtgcaacttc
      781 gagctcctcg acacgctgcg cctggaacga ctcgacgtga ccgaagccgc gcggctggtc
      841 gaacacgtcg cgcgccgcca gcaagtcgca accagcgaag ccgcacgcga tctgcttgtg
      901 caacagttcg ccggcagtcc atttttcatc agcatctttc tgcaggccgc gcgtgaaaaa
      961 cacacgtcgc tgctttctta tctcgattgc gaacgtctct acgtggacga gctcttcggc
     1021 ggtcacattc atcgtcactt cgccgatctg ctcgaagaga tttcaccgcg gctcgacacg
     1081 cggcatctct taatccgcct gctttgggaa gcagctgccg gcgaagaaca gacgtcgtca
     1141 ttcgaagttt ggcgaaagcg tctgcacatg actgctactg agctcgaaga catgttgcac
     1201 cgcctgcacg tacaggagtt tgtaaactgg gacgaaacga cagtcgaagc cggcgctgga
     1261 ccacaggcgt ggaaagacta tctgaagatt cgctatcgcc tcgacgtgct caacgaaccg
     1321 cgcgccctcg tcgtcgccga catgctcgcc gactctctca aacgcgcccc acacacgatg
     1381 gcgcgtcact acaagcaaat cgcgaacgcg ggtctgcgcg atctgatttc ccgtttcaat
     1441 actcagcgag tacccgcggt gcttttcgac tacaaaactt tcagcaacaa gtacaagggc
     1501 gcggaaagcg aaacgatcgt ctccggcctc gaggcggaga ctgatttcgt aaaactgcca
     1561 cagacggtcc acctcgcgag ctgttcggct ttcatcagcg acatgcgcca gatctgcgaa
     1621 gaggaccatt gtctgatagc acacacattc gaagagggaa gctactcaga cgcgcaacag
     1681 gtgatctggc tggccgcgga catcgagtcg aagctggaag tcgacgtcga cgtcgcacgc
     1741 gtctggtgcg agcggctcga gaatatcgcc gtccggctcg gcttcagtcg ttttcagatc
     1801 tggttaatct caaacgaagg attttctgat gaggccagga gtctcctggt ccgccacaaa
     1861 gcctttagct cgagccggca gcaactaggc ctggtggcgt ctcgccttgg agagactgcc
     1921 cccagtcctg tagtaccagc cggtccaccg gatgagtttc tgatggtagt gccgatgggc
     1981 gaggataatg agctgattgc ggcaaacacc gccgagcaga ttgccaagcg gctgaccttc
     2041 cgcccggagg ccatcaacca gataaaaacg gcgattgtag aggcttgtat caacgcttcg
     2101 gagcacagtt tcagccctga tcgaaaaatt tatcagcgtt ttcgcgtcga aagtgataga
     2161 ttagtcatca caatttcgag tcgcggtatc gtgccagcta atctgaatgg cgcgaatagc
     2221 ggtcaagaag cgacagaagc ggcagaagaa agacgcggtt ggggccttaa actcatccga
     2281 actctcatgg atgaggtcga atttgagcgg gtcgacgagg gcaccagttt gcgcatgacc
     2341 aagtacttgc gacctcaaaa aagtacttaa agcgcaaact attcacaaca gacttacgga
     2401 aacaaaactt aatttcggct tttaagcttt gtagcaacat tcgaaggagg gagctttcga
     2461 gtggatacac caagtggcaa gctagtacgc tcgcactgtg ttggcaccac tgcggttgtt
     2521 tacgctagcg attaccttaa taagctgacc ggtgaaaaga tcgagcgcga gtgtcgccgg
     2581 cacctcaact gcggttgccg tgccctcgtg atcgattttg gtgataccaa gctggtcaat
     2641 agcatcggta tctcaatcct cctgggaatc attgacatcg ctgagaagaa cgacgcccgg
     2701 attatctttt cagaagtcaa ctcccagaca aagcagttgt tcgaaatgct cggactcacc
     2761 cggcatgttg cacttgcgaa agacgagcgg gaagcgctct tgaacatttc gcgttgcgag
     2821 actactccca tagtccactg acaatggagc tgtcgaccca gaaactcatt catacatttg
     2881 gagcgctggc cgatctaggg caggaaattg ctggaaccgg cgacttcacc gaaatggtga
     2941 ggacgtcgat gcatttgctg ttaggaacgc tggcgttgcg gcgtggcgct gtcgctgaat
     3001 gcactgggca tggtccaata ggaccattga agtgtgtagc cgtctggggt ctcggcgatc
     3061 agatattcaa cgctctcgag atcgaagacg cggacaagca ggcttttctg tccggagaag
     3121 caacggaatc acttgtcaga aacaccacca gcacgaacgg ctttctgaat cgacatcgcg
     3181 acaagctcgc cgccgaagga atcgaactgg tgtttccgat ggtcgtgcgc ggggaagtga
     3241 ctggcctgat actgctcgga ggcaaagcca gcggcgaaga gttctccacc gacgacttcg
     3301 agacgattcg cgcgatggtc cggcacatcg gggtcggtat tcacacgcat cgcctgctgg
     3361 agcaagttgc gcagcgcgcc gaggagaatc gcagactgta cgacgagctg cgcgcaatct
     3421 atcgcgacac agtccgggcg ttcgcggcgg cgatcgatat caaagacaag tacacgcagg
     3481 gccactcgga gcgcgttggc
//
LOCUS       DASTEY010000097         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_364622, whole genome shotgun sequence.
ACCESSION   DASTEY010000097 DASTEY010000000
VERSION     DASTEY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364622"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFO72_01665"
     CDS             <1..369
                     /locus_tag="VFO72_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9478021.1"
                     /translation="ALPAPEAVRPDLPQAFVAPRAGVQQSLAGIWSDLLGLPQVGIHD
                     NFFDLGGHSLLTTQLISRVRELFKVELPLRQVFQQPTIAALAIVIEQAQNNGTPNEIP
                     KIVRVDREARRLARPVRSLQ"
     gene            412..>2004
                     /locus_tag="VFO72_01670"
     CDS             412..>2004
                     /locus_tag="VFO72_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9478022.1"
                     /translation="MLPSVTPDEVFVFPLSFAQQRLWFLHQMDPSNAAYNMPLAFRLS
                     GQLEVEALQWSLDEIVRRHEILRTTFDVLDQAPMQLVAATGDLPLAVTDLSSLPPAVS
                     EVEAERLASDETQRPFDLVNGPLIRARLMRLGANEHVLLVTMHHIISDGWSQTIVLNE
                     LSALYEAAVAGRSSPLPELSIQYADFAEWQREWLSGELLDQQLEYWRKHLQGAPPSLE
                     LPTDRPRPAVQSFNGARHSFALSRDLSARLVELGRREDATPFMTLLAAVYVLLYRYTG
                     ETDIVVGTPIANRNRHELESLIGFFVNTLALRVDLSGNPTFRELLARVRDVSLGAFEH
                     QDLPFEMLVEELSPIRDQSRNPLFQVMFALQNTPEAASLQFDLRIRPIDVKTAGAQFD
                     LTFDVTEVDGQFDVSLVYNTDLFDASTIELTGERFKVLLAGVVHSASRPISTLPVLTA
                     PESRRLLVEWNDTAKELTGEVCIHRLFEQQVERTPEAIALSFEERRMTYEELNHRANK
                     LAHHILTLGVGQESFVGLLLERS"
BASE COUNT          400 a          590 c          562 g          452 t
ORIGIN      
        1 gcactcccgg cgccggaagc tgtcaggccg gatttgcccc aggcattcgt cgcaccgcgt
       61 gccggcgtcc aacagtcgct cgccggcatc tggtcagact tgcttggcct cccgcaggtc
      121 ggcatccacg acaatttctt cgatctcggt ggtcactcgc tgctcacgac tcagctcatc
      181 tctcgcgtgc gcgagttgtt caaggtggaa ctaccgttgc gccaggtgtt ccaacaacca
      241 actatcgccg cgcttgcaat cgtcatcgag caggcacaaa acaacggcac gccgaacgaa
      301 ataccgaaga ttgttcgcgt agatcgagaa gcgcgacgtc tggcgcgacc cgtgagatcc
      361 cttcaatgac ttcttttatg tgccctttgt gccttttttc ggctaagtct tatgttaccc
      421 agcgtgacgc ctgacgaagt cttcgtcttt ccactctcct tcgcccagca acgtctgtgg
      481 ttcctgcatc agatggatcc gtcgaacgcg gcctataaca tgccgcttgc tttccggtta
      541 tcagggcagc tcgaggtgga ggccttgcaa tggtctctcg acgaaatcgt tcgccggcac
      601 gagatcctgc gcactacatt tgacgtgttg gaccaggcgc ctatgcaatt ggtcgctgcc
      661 actggagacc tgccgttggc tgtcacagat ctcagctcgc tcccaccagc ggtcagtgaa
      721 gtagaagccg agaggttggc gagcgacgag acacaacggc cattcgatct agtcaatggt
      781 ccgctcattc gtgcccggtt gatgcggttg ggagctaatg aacacgtgct gctcgtgacg
      841 atgcatcaca tcatcagcga cggatggtca caaacgatcg tgttgaacga gttgagcgcg
      901 ctttacgaag ccgccgtcgc tggccggagc tcgccgttgc cggaattgtc gatccagtac
      961 gctgactttg ccgagtggca gcgcgagtgg ttaagtggcg agctattgga ccaacagctc
     1021 gagtactggc gaaaacattt gcagggcgcg ccgccgtcgc tggaactgcc aactgatcgg
     1081 ccacgacctg cagtgcaaag cttcaacggc gcgcgacatt cgtttgcgct ttcgcgtgac
     1141 ctatcggcgc gtctggtgga gttgggtcgc cgtgaagatg cgactccttt catgacgctc
     1201 ctggccgcgg tctatgtgct gctttaccgt tacacaggcg aaactgacat cgtagttggc
     1261 actccgatag ccaaccgcaa ccgccatgag ctcgagtcgc tgatcggctt cttcgtcaat
     1321 acgctggcct tgcgcgtgga cctttccggc aatcccacgt tccgcgaatt gttggcgcgg
     1381 gtgcgcgacg tctctcttgg cgcattcgag catcaggact tgccgttcga gatgctggtt
     1441 gaggagttga gtccgattag agaccagagc cgcaatccgc tctttcaagt aatgtttgct
     1501 ctccagaaca cacccgaggc tgcttcactg caatttgatc ttcgcatcag gcccatcgat
     1561 gtaaagacag cgggcgcgca gtttgatctc accttcgacg tgactgaagt tgacgggcag
     1621 ttcgatgtct cgctcgttta caacacggac ttgttcgacg cgtcgacgat cgagctaaca
     1681 ggcgaacgtt tcaaagtctt gctcgcaggc gtggtgcaca gcgccagccg gccgatcagc
     1741 acgctgccgg tactgaccgc gcccgagtca cgacgattgc tggttgagtg gaatgatacc
     1801 gcgaaagaac taacaggcga ggtctgcatc catcgacttt tcgaacagca ggtggaacgc
     1861 acccccgaag ccattgccct ctcattcgaa gaacgccgaa tgacttacga ggagttgaac
     1921 caccgagcca acaaactcgc gcaccacata ctcactctgg gtgtcggaca agagtcgttt
     1981 gtcgggttgc tgttggaacg ttcg
//
LOCUS       DASTEY010000098         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1205161, whole genome shotgun sequence.
ACCESSION   DASTEY010000098 DASTEY010000000
VERSION     DASTEY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205161"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFO72_01675"
     CDS             complement(<1..431)
                     /locus_tag="VFO72_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478023.1"
                     /translation="MRVLIAAGGTGGHIYPGIAVAQEIVRRDADSKVRFVGTARGLET
                     RLVPQAGFELTLIDSAGLKNVSLGARMKGLAILPKSFVSARRVIREFRPEVVVGAGGY
                     VSGPVVLTAALLRRPTLVMESNALPGWTNRVLARFVDRAAVS"
     gene            complement(428..1612)
                     /gene="ftsW"
                     /locus_tag="VFO72_01680"
     CDS             complement(428..1612)
                     /gene="ftsW"
                     /locus_tag="VFO72_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9478024.1"
                     /translation="MARKLAPDKWLFAATTGLALFGVVMVYSASAVLAAKENGNQFYY
                     VLKQGVWVAIGFIVMLLMMQFNYQQLKNRRVVYGLLLVTSVALIAVFGFSSSNGAHRW
                     IKLPGFSLQPSEVSKLALIIFLAYFLEKRAGEEGDFWRTFVPCGLVTGWLAFLIVIEP
                     DFGTAMMLALIFIVVVYTAGARLLHLAMAAAPALLVAMGLLIFVPWRLKRLITFLDPW
                     ADPQGAGFQVVQSLIAIGSGGPQGLGFAQGKQKMLFLPYAHSDFIFAVVGEELGLVGA
                     LTVVTVFALFLWRGVRTALLAPDRFGMLLSLGIVTSIVSQALFNISVVLSLVPTKGIP
                     LPFISYGGSSLVPTLAAVGILLNVSQHASGGITDFRLPIAKLTEKYEGRPNRQSAIVN
                     WQ"
     gene            complement(1666..>2500)
                     /gene="murD"
                     /locus_tag="VFO72_01685"
     CDS             complement(1666..>2500)
                     /gene="murD"
                     /locus_tag="VFO72_01685"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9478025.1"
                     /translation="FHPSIAVVLNVTPNHMDRYETFNDYAAAKHRIFMNQVEGDVAVL
                     NADDPTVSSWASGLRAKVMSFSVRQELERGVFLSGDELVFRWDEGEQDLLRISEMKLR
                     GLHNVENVAAALTAGIAAGAQIESMRETVKQFNPVEHRLEFVNEIRGVRFYNDSKATS
                     VDATLKALEAFASDPGQVVLILGGRGKKAPYAPLEPLVRSKVRKLILIGEDAETIANE
                     LGEYAPFERAASMQEAVATSLASAEPGDVVLLAPACASFDMFDSFEHRGKVFKKEVAN
                     L"
BASE COUNT          607 a          804 c          601 g          488 t
ORIGIN      
        1 gacaccgcgg cacgatccac aaaacgagcc agcacgcggt tggtccaacc aggcaacgcg
       61 tttgactcca tcaccaatgt cgggcgacgc agcagcgccg ctgtcagcac caccggtccg
      121 gagacgtaac cacccgcacc aaccactact tccgggcgaa actctcgaat cacgcgtcgc
      181 gccgaaacaa aacttttcgg caggatcgcc agccccttca tgcgtgctcc gagactgacg
      241 ttcttcaaac ccgcactgtc aatgagcgtc aactcgaatc ccgcctgcgg gaccagtcgt
      301 gtttccaaac ctcgcgccgt tcccacgaag cgaaccttcg aatcagcatc gcgtcgcacg
      361 atctcctgtg cgacagcgat tccgggataa atgtgtccgc cagtgccacc ggccgcgatc
      421 agcactctca ttgccaattg acaattgccg attgccgatt tggacgccct tcatacttct
      481 cagtcaactt ggcaatcggc aatcgaaaat cggtaatgcc cccgcttgcg tgctgcgaca
      541 cgttgagcag aatgccgacc gcggcgagtg tcggcacgag cgacgagccg ccgtaagaaa
      601 taaacggcaa cggaatgcct ttcgtcggta cgagcgagag caccacgctg atgttaaaga
      661 gcgcctgcga gacgatgctt gtcacgatgc ccaaactcag caacattccg aatctgtccg
      721 gcgcgagcaa cgccgtgcgc actccgcgcc acaaaaacaa tgcaaacaca gtgacaacgg
      781 tcaacgcgcc gaccagccca agctcctcgc caaccaccgc aaagataaaa tccgaatgcg
      841 cgtagggcag aaacaacatc ttctgtttgc cttgcgcaaa gcccagcccc tgtggtccac
      901 ccgagccgat cgcgatcaac gactgcacca cctgaaaacc cgcgccctgc ggatcggccc
      961 aggggtcgag aaacgtgatc agacgcttca accgccacgg cacaaagatc aacagcccca
     1021 tcgctaccag caacgcgggc gctgccgcca ttgccaggtg caacaatcgc gcacccgccg
     1081 tgtaaacgac gacgatgaag attaatgcca gcatcatcgc cgtgccgaag tccggttcga
     1141 tcacgatcaa aaacgccagc cagccggtga ctaatccgca gggaacaaac gtccgccaga
     1201 agtctccctc ttcgcccgcg cgtttctcca ggaagtaggc caggaaaatt atcaatgcca
     1261 gcttcgaaac ctctgaaggc tggagcgaaa aacccggaag tttgatccag cgatgggcgc
     1321 cgttgctgct gctgaatcca aatacggcga tcaatgcgac gctcgttact aacagcagac
     1381 cgtaaacgac gcgacgattc ttcagttgtt gatagttgaa ctgcatcatc aacaacatga
     1441 cgatgaaacc gattgccacc cacacgccct gcttcagcac gtagtagaac tggttgccgt
     1501 tctcctttgc ggccagcacg gctgacgcgc tgtagaccat gacgacgccg aaaagcgcaa
     1561 ggccggtggt cgccgcgaag agccatttgt ctggagcgag tttgcgtgcc attggtaagt
     1621 aggacagact ttctagtctg tctttgacag gcaagaatgc ctgtcctaca gattcgcgac
     1681 ttctttctta aaaactttcc ctcgatgttc gaaactgtca aacatatcga aactcgcgca
     1741 tgccggagcc agcaagacaa cgtcacccgg ttcagcagaa gcaagagacg tcgccacagc
     1801 ctcttgcata ctcgcagctc gctcaaacgg cgcgtattcg cccaactcgt ttgcgatcgt
     1861 ctccgcgtct tctccgatca gaatcagctt acgcactttc gatctgacta gcggttccag
     1921 cggagcgtac ggcgctttct ttcctctacc accaagaatc aacacaacct ggcccggatc
     1981 gcttgcgaat gcttcgagcg ccttcagggt cgcatcaaca ctggtcgcct ttgagtcgtt
     2041 gtaaaacctc acacctcgaa tctcattcac gaactccagc cggtgctcga cgggattgaa
     2101 ctgcttcacc gtctcgcgca tcgattcaat ctgcgcgccc gccgcgattc cggccgtaag
     2161 tgcggcggcc acgttctcga cgttgtgtaa cccgcgcaac ttcatctcac taatccgcaa
     2221 taaatcctgt tcgccttcat cccaacgaaa gacgagttca tccccgctca gaaaaactcc
     2281 tcgctcgagc tcttgtctga cactaaagct catcaccttt gctcgcagcc cactcgccca
     2341 tgaagaaact gtcggatcgt ccgcgttgag caccgccacg tctccttcaa cctggttcat
     2401 gaagatgcga tgttttgccg cagcgtagtc attgaacgtt tcgtaacggt ccatgtggtt
     2461 gggagtcaca tttaaaacaa cagcaatcga cggatgaaat
//
LOCUS       DASTEY010000099         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_677861, whole genome shotgun sequence.
ACCESSION   DASTEY010000099 DASTEY010000000
VERSION     DASTEY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677861"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /gene="ppdK"
                     /locus_tag="VFO72_01690"
     CDS             complement(<1..1892)
                     /gene="ppdK"
                     /locus_tag="VFO72_01690"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9478026.1"
                     /translation="MSTVTEETPEITEPKTNKWVYLFDEGPGDDKTLLGGKGAGLCEM
                     TQTGLPVPPGLIVTTEACNAFFDNDKNFPEGMWEQVKEGLKKIEEKVGKRFGDPKNPL
                     LVSVRSGAAFSMPGMMDTVLNLGLNEETVQGLAEQTGDLRFALDAYRRFASLFGEIVM
                     GVAHEKFERVLDRFKAQTQGGRDTDLAADHLREIIAAEKQIILTEQSAIPEDPYEQLR
                     VAIAAVFNSWMGRRAIDYRRVNRIPDSLGTAVNVQAMVFGNMGAGSGTGVAFTRNPSN
                     GRKELYGEYLLNAQGEDVVAGIRTPNPISQLKKELPEVYDQFASIAGLLEQHYGDMQD
                     CEFTIERGKLWMLQTRTGKRSGAAAVRIAVEMVFEKLIDKATAVKRVTPEQLDQLLHP
                     TVDPKTNATVLATGLPASPGAAQGRVVFSPDEAEEMARDGAHVVLVRQETSPDDFHGM
                     VAAQAIVTARGGMTSHAAVVARGMGKTCVSGANALNIDYGQQQFTVDGTVVTKGEWIT
                     VDGSTGRVFLGQVPTIQPTLGNDFYKLMTWADQFRRLRVRANADTPLDAKTARQFGAE
                     GIGLCRTEHMFFGDQRLAAMREMILSEGVGAREKALEKLLPLQRGDFIGIFREMAGLP
                     VTIRLL"
     gene            1976..2455
                     /locus_tag="VFO72_01695"
     CDS             1976..2455
                     /locus_tag="VFO72_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9478027.1"
                     /translation="MANVTSLNVNGKKLRVDVDSQTPLLNVLRDHLNLTGTKYGCGEA
                     RCGACTVLIDGQMMRSCVTPVGRVGGKQVTTIEGLEKDGKLHPLQEAFLKADALQCGY
                     CTPGMIMSAASLLNKISKPSREAIVKHMDANVCRCGTYARIVNAIEIAAGTAKEVAE"
     gene            2452..>2961
                     /locus_tag="VFO72_01700"
     CDS             2452..>2961
                     /locus_tag="VFO72_01700"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9478028.1"
                     /translation="MNDDLELERYELFEGPSYNFNFNRRQFLKAFSGGIALIVPMSNL
                     VARTLQQDESGSGRGNQRVPDEIGAWIHIDENGIVSVFTGKVELGQNIRTSLAQAVAE
                     ELHVPVTTVRLVMADTDLTPFDMGTFGSLTTPRMAPQLRKAAAAARETLISLAAEQWK
                     VEPTAVRIVN"
BASE COUNT          607 a          875 c          845 g          634 t
ORIGIN      
        1 tcgagcagcc ggatggtcac cggcagtccg gccatctcac gaaagatgcc gatgaaatca
       61 ccacgctgta gcggcaacaa cttttcgagc gccttttcgc gcgcgcccac gccttccgaa
      121 agaatcatct cgcgcatagc cgcgagccgc tggtcgccaa agaacatgtg ttccgtgcga
      181 cacaagccaa taccttcggc gccaaactgt ctggctgtct tcgcgtcgag cggcgtatct
      241 gcgtttgccc tgacgcgcaa ccgtcgaaac tgatccgccc aggtcatgag cttgtaaaag
      301 tcgttgccga gcgtcggttg aatcgtcggc acttgtccga gaaacacgcg accggttgaa
      361 ccatcaaccg tgatccattc gcccttcgtc acgaccgtgc cgtcgacggt gaactgttgc
      421 tggccgtagt cgatattcaa agcgttggcg ccgctgacgc aggtcttgcc catgccgcgc
      481 gcgaccaccg ccgcgtggct cgtcatgccc ccacgcgcag tgacgatcgc ctgtgctgca
      541 accatgccgt gaaagtcatc cggactggtc tcttgacgca ccagcaccac gtgcgcgcca
      601 tcgcgcgcca tctcttctgc ttcgtcggga ctgaacacaa cccgtccctg cgccgcgccc
      661 ggactcgccg gcagacccgt cgcgagcacg gttgcgttgg tctttggatc gactgtcggg
      721 tgcaaaagtt gatcgagctg ttcgggcgtg acgcgtttca cagccgtggc tttgtcgatc
      781 agtttttcga aaaccatctc gaccgcaatg cgcacggcag cagcaccact gcgcttgccc
      841 gtgcgcgttt gcaacatcca cagcttgccg cgctcgatcg tgaactcaca gtcctgcatg
      901 tcgccgtaat gctgttcgag taatccggcg attgaagcaa actgatcgta gacctcgggc
      961 aattcttttt tgagctggct gatcggattc ggcgtgcgaa tgccggcgac cacgtcttcg
     1021 ccctgcgcgt tcagcagata ctcgccgtag agctctttcc tgccgttcga aggattgcgt
     1081 gtaaacgcca cgccggtgcc gctacccgcg cccatgtttc caaacaccat tgcctgcacg
     1141 ttgactgcgg tgcccaaaga atcgggtatg cggttgacgc gccggtaatc gatcgcgcgc
     1201 cggcccatcc acgagttgaa taccgcggcg atcgcgacgc gcagttgttc gtatggatct
     1261 tcaggaatcg cagactgttc ggtgagaatg atctgcttct cggcggcaat gatctcgcgc
     1321 aagtgatcgg cggcgagatc cgtgtcgcgc ccaccttggg tctgcgcttt gaagcgatca
     1381 agcacgcgtt caaacttctc atgagcgacg cccatgacga tctcgccaaa cagcgacgca
     1441 aaccgccggt aagcatcgag cgcgaaacgc agatcgccgg tctgttcggc gaggccctgc
     1501 acggtctctt cattcaaacc gaggttcaag actgtatcca tcatccccgg catcgaaaac
     1561 gccgcgccgg aacgaactga aacgagcagc gggttctttg gatcgccgaa acgtttgccg
     1621 accttctcct cgatcttttt caaaccttcc ttgacctgtt cccacatgcc ttcgggaaag
     1681 tttttgtcgt tgtcgaagaa cgcgttacag gcttcggtgg tgactatcaa accaggcggc
     1741 acgggcaatc cggtctgcgt catttcgcag agcccggcgc cttttccgcc gaggagtgtt
     1801 ttgtcgtctc cgggtccctc gtcgaaaagg tagacccact tattcgtttt tggctcagtg
     1861 atttccgggg tttcttcggt tacggtactc atggcggttg caagttaacg tatttcttct
     1921 tatttatcca cagacgaatg cggctggcac agatcagatt acaataaatc ccactatggc
     1981 aaatgtaacg agtctgaacg tgaacggaaa gaagctgcgc gttgatgtcg attcgcaaac
     2041 acctctgctc aacgttttgc gcgatcacct caacctgacc gggacgaaat atggttgcgg
     2101 cgaagcgcgc tgcggcgcgt gcacagtgct catcgacggg caaatgatgc gttcgtgcgt
     2161 gacgccggtc gggcgagtcg gaggcaagca agtcaccacg atcgaagggc ttgagaaaga
     2221 cggcaaacta catccgttgc aggaggcttt tttgaaagcc gacgcgctcc agtgtggcta
     2281 ttgcactccg ggaatgatca tgtcggcggc cagcttgttg aataagattt cgaagccttc
     2341 acgcgaagcg atcgtgaagc acatggacgc caacgtttgc cgttgtggca cttacgcgcg
     2401 catcgtcaac gcgatcgaga ttgcagcggg cacggcgaag gaggtggccg aatgaacgac
     2461 gatctcgaac tagagcggta tgaactcttc gaagggccaa gttataactt caacttcaat
     2521 cgccgtcagt ttctcaaagc gttcagcggc ggcatcgcac tcatcgtgcc gatgtcgaac
     2581 ctcgtcgcgc gaaccctaca gcaggatgaa tcgggaagcg gtcgcggaaa ccagcgcgtg
     2641 cccgacgaga tcggcgcgtg gatccacata gatgaaaatg ggatcgtttc agtgtttact
     2701 ggcaaggttg agttgggtca gaacatccgc acgtcgcttg ctcaggccgt ggcggaagag
     2761 ctgcacgtgc cggtgacaac ggtgcggttg gtgatggccg acacggatct gactccgttt
     2821 gacatgggca cgttcggtag cctgacaact ccgcgaatgg cgccgcaact tcgcaaagca
     2881 gcagcggccg cgcgcgagac tctgatctcg ctggccgcgg agcagtggaa agtcgagcca
     2941 accgcggtgc gcatcgttaa t
//
LOCUS       DASTEY010000100         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_2240, whole genome shotgun sequence.
ACCESSION   DASTEY010000100 DASTEY010000000
VERSION     DASTEY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VFO72_01705"
     CDS             complement(<1..1425)
                     /locus_tag="VFO72_01705"
                     /inference="COORDINATES: protein motif:HMM:NF037521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HET9478029.1"
                     /translation="MSLLIANGYWPLEKSQPIVEKTQTIRLAPDTSKLSEGERKAVAK
                     LIEVGQIFQKLYEQQRHPEALSSYRALEQLHKQNGGAPQTQNLLTLYRLFQGPIATTL
                     ENKREPFLPVKPLKPGKNMYPWGITKEEVERFINSSGNPEQTRSEIMDLRTVVRVASA
                     KNLREDIARLNKYPVLQTLHPKLMGQLQKLAQQPAPNNKMVLYAVPYALAYADELIKS
                     HGLLNEAADAVEKDDEEFARFLRNRARDLLSNDYESGDASWVTGRFKNLNAQIGAYET
                     YDDELFGVKTFFSFVLLQSRQQETIALRQAMKGLQALEDSLPYQNHKKVREDIPVGVY
                     DVVADFGQSRGGNTATILPNESYLARRYGRTIMLRTNIMRDPNIFESTSKTFAAAVGE
                     QQAKELTSEGNFYRTLWHEVGHYLGVDRTKDGRDLDEALQDNANTMEEMKADLVSLFV
                     AGALHKQGYYTDAQLRSVYAGGILR"
     gene            1611..2351
                     /locus_tag="VFO72_01710"
     CDS             1611..2351
                     /locus_tag="VFO72_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9478030.1"
                     /translation="MTALLTMLQRRFRRMRRRRKVGRAYDMALEVARVLPPHADVLDV
                     GCGNGFIAHHLTSLLGKPVLGLDVGDSTAAHIKYLPYDGRHFPVRDQSFDAVLLCYVL
                     HHAQDARLVLNEVSRVLREGGLAIIYEDIPNLWWDRVVCWTHDRKWRGRTGPCTFQRE
                     HGWRKTFNLAGFEIVNERALSRWRNLAHPVSRRFFVCRTGIPASHATALYACHRQAGM
                     PVLLKPDHAALPQLLDHSQAVSLAKGTD"
     gene            complement(2274..3401)
                     /locus_tag="VFO72_01715"
     CDS             complement(2274..3401)
                     /locus_tag="VFO72_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9478031.1"
                     /translation="MAIKGTLKTMSLTDLLQFLAAGRKSGTLKFDQGKITKQVYFKNG
                     SIVGSKSNDPREYLGQVLLHYGKVDDNQLKAAREAQRTSGAKLGEVLVAQGSLTEAEV
                     LDILKTRTLDAIYDLFMWTEGDFEFYDEDPLPDDLLPIEVEPTNVIMEGIYRIDELAR
                     YRTLVPSDRAILELAAGWTSALTKLGKEFRQILVFIEKRMSVAEICYHMHASAFHVYG
                     QLYTLISEGVARVVGEKPEGSPAPGSELEDLPESVTEMVWSAERKLESDPEAALQIIQ
                     AALQQQPNNPEAHSLLPTAEEKYVKHVYATGGLSPRSVPQLRISPAELTNMEIDPQEG
                     FLLSRINGSWDVQSILSICPFREADCLRMIKKLRERGVIGF"
     gene            complement(3460..3813)
                     /locus_tag="VFO72_01720"
     CDS             complement(3460..3813)
                     /locus_tag="VFO72_01720"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9478032.1"
                     /translation="MDERRSKPRLSVHLDAVWQGGEERHSARVTDLSEGGCYLDTVGE
                     VMVGEIVAFRVLLPDDEWLYLEGEVRHHRHGLGFGVQFVDLNEEQTENLRRLLAIARE
                     AGPEAPAISVDLVED"
BASE COUNT          807 a         1169 c         1084 g          966 t
ORIGIN      
        1 gcgcagaatt ccgccggcgt acacacttct taactgcgcg tcagtgtagt agccctgttt
       61 gtgcagcgct ccagccacga agagcgaaac aagatccgcc ttcatctctt ccatcgtgtt
      121 cgcgttgtcc tgcagcgctt cgtcgagatc gcggccgtcc tttgtgcgat cgacgccgag
      181 gtagtggccg acttcgtgcc acagcgtccg atagaagttg ccctccgaag taagctcttt
      241 tgcttgctgt tcgcccaccg cggcggcaaa cgtcttcgaa gtgctctcga agatattcgg
      301 atcgcgcatg atgttggtgc gcagcattat tgtgcggccg tagcggcgcg cgagatagct
      361 ttcgttcggc agtatcgtcg ccgtgttgcc gccgcgcgat tgaccaaagt cagcgaccac
      421 gtcgtaaacg ccaaccggta tgtcttcccg caccttcttg tggttctggt atggcagcga
      481 atcttcgagc gcctgtaaac ctttcatcgc ctgccgcagc gcaatggtct cctgctggcg
      541 actttgcaac aggacgaacg aaaagaaagt cttcacgcca aacagctcgt cgtcgtaagt
      601 ctcgtacgct ccaatctgcg cgttgaggtt cttgaatctg ccggtcaccc aacttgcatc
      661 accggattcg taatcgttcg acaacagatc gcgcgcgcgg ttgcgcagaa agcgcgcaaa
      721 ctcttcatca tccttctcaa ctgcgtccgc agcctcattg agcaagccat ggctcttaat
      781 caactcgtct gcataagcca acgcatacgg cacggcgtac aaaaccatct tgttgttcgg
      841 agccggctgt tgtgcaagct tttgtaactg gcccatgagc ttcggatgaa gcgtctgtag
      901 cacgggatat ttgttgagcc gagcgatgtc ttcacgcaga ttcttggcac tcgcgacgcg
      961 gaccaccgtg cgcaggtcca tgatctcgct ccgtgtctgt tccggattac ccgacgagtt
     1021 gatgaaccgc tcgacctctt ccttcgtgat tccccacggg tacatgttct tgccgggctt
     1081 caatggctta accggcagga acggctctcg tttgttctcg agtgtggtcg cgatgggccc
     1141 ctgaaagagc cggtatagcg tcaacagatt ttgcgtttgt ggcgcgccgc cgttctgttt
     1201 gtgcaactgt tcaagcgcgc gatacgacga gagcgcttcg ggatggcgct gttgttcgta
     1261 gagcttctga aagatctggc ccacttcgat cagtttggcg accgctttgc gctcgccttc
     1321 cgagagtttg gaagtgtctg gcgccagacg gatcgtttgg gtcttctcga cgatcggctg
     1381 gctcttttcg agcggccagt aaccgttggc aatgagcagg gacatgatta tgcctgaaac
     1441 gaatttagtc attagcacct ccgtcacaga ttaaacccga ttacaccgag ccgaaacaat
     1501 ccgtgtcaat ccgtgttgcc atctgtcggc ctttttctgc gctggtcgaa gactgactta
     1561 aggttttcag cgtcaaacgt tgaggcattg tcgttgcaaa cgcccccgta gtgaccgctc
     1621 tactcacaat gctgcaacgc cgcttcaggc gtatgcgacg gcgaaggaag gtcggccgcg
     1681 cctatgacat ggcgctcgaa gtcgcccgcg tgctaccgcc acacgctgac gtactcgacg
     1741 tcggttgcgg caacggcttc atcgcacatc acctcaccag cttactcggt aaaccggttc
     1801 tcggactcga cgttggcgac agtaccgcgg cgcatatcaa atacctgcct tacgacggtc
     1861 gccattttcc cgtgcgcgat cagagtttcg acgccgtgtt gctctgttac gtcttgcatc
     1921 acgcgcagga cgcacggttg gtgcttaacg aagtgagccg tgttttgcgc gaaggcggat
     1981 tggcgatcat ttacgaagac ataccaaacc tctggtggga ccgagtggtc tgttggacgc
     2041 acgatcgcaa atggcgtggc cgcaccgggc cgtgcacgtt tcaacgtgag cacggctggc
     2101 gcaagacgtt caacctggct ggttttgaaa ttgttaacga gcgggcgtta tcgcgttggc
     2161 gtaacctggc ccatccggta tcgcgacggt tcttcgtatg taggacaggc attcctgcct
     2221 ctcatgcgac cgcgctttac gcctgtcacc gacaggcagg aatgcctgtc ctactaaaac
     2281 ccgatcacgc cgcgctcccg caacttcttg atcattcgca ggcagtcagc ctcgcgaaag
     2341 ggacagattg agagtatcga ttggacgtcc catgaaccgt tgattcgcga gagcaaaaaa
     2401 ccttcctgcg gatcaatctc catatttgtt aactcagccg gcgagatccg caattgcgga
     2461 actgaacgcg gcgacagacc gcctgtcgcg tacacgtgct tgacgtactt ttcttcggcc
     2521 gtgggcaaca gcgaatgggc ctcggggttg tttggttgtt gctgtaaagc tgcctgaatg
     2581 atctgcaacg cagcttccgg atcgctttcg agtttccgtt ccgcagacca gaccatttcc
     2641 gtcacgcttt ccggcagatc ctcgagctca ctacctggcg cggggctgcc ttcgggcttc
     2701 tcaccgacaa cacgtgcgac gccctcgcta atcaacgtgt agagttggcc gtagacgtga
     2761 aacgccgaag cgtgcatgtg ataacagatc tccgcgacgg acatccgctt ctcgataaaa
     2821 acgaggattt ggcgaaactc cttgccgagc ttcgtcaaag ccgacgtcca gccggcggca
     2881 agttcgagaa ttgctctatc ggacggcacc agcgtgcgat aacgcgcgag ttcgtcgatg
     2941 cgatagatgc cttccatgat gacgtttgtc ggttcaactt cgatcggcag caggtcgtca
     3001 ggcaatggat cttcgtcgta aaactcgaag tcgccttcgg tccacatgaa caggtcgtag
     3061 atcgcatcca acgtgcgagt cttgaggatg tcgagcactt ccgcttccgt taaagatcct
     3121 tgtgcgacta gcacttcgcc aagtttcgcg cctgacgtac gttgcgcttc gcgcgcggct
     3181 ttcagttggt tgtcgtcaac ttttccgtag tgcagcagta cctggcccag gtactctcgc
     3241 gggtcgtttg acttcgaacc tacgatcgag ccgttcttga agtaaacctg cttggtgatc
     3301 tttccttgat caaacttgag cgttcctgac ttccgtcccg ctgctaaaaa ttgcagcagg
     3361 tcagtcaggc tcatcgtttt caaagtacct ttaatagcca ttcgttttgt ttcaggcact
     3421 acctggcgcg ggccgcacgg tgaggctctc tgggatttat taatcttcga cgaggtctac
     3481 cgagatcgcg ggcgcttccg gaccagcctc acgcgcgatc gccagcagac ggcgcaagtt
     3541 ctccgtctgt tcttcgttta agtctacaaa ttgcactcca aaaccgagac catgacgatg
     3601 atgcctgacc tcaccttcca gataaagcca ctcatcgtca ggaagaagca cgcggaaggc
     3661 gacgatctct cccaccatca cctcgcccac tgtgtcgaga tagcaacctc cttctgaaag
     3721 atccgtgacg cgcgccgagt ggcgctcttc gccaccctgc caaaccgcgt cgaggtgtac
     3781 gctcaaccgg ggtttagatc gtcgttcatc catggcctga agccatcata gattttttag
     3841 tgaggcacgg aaagagaaat ctaagtgggg gctcacatac aacgcacacg cagcgctgcg
     3901 ggaattatct ggaatgtggc tggaagcttt ccaggaagtt ctccatccac ttccagatct
     3961 attatcgagg cgcgttcagc gggccggaca gtgacttttc gcgccagcgc gtggctcact
     4021 tccggc
//
LOCUS       DASTEY010000101         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_167124, whole genome shotgun sequence.
ACCESSION   DASTEY010000101 DASTEY010000000
VERSION     DASTEY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167124"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /locus_tag="VFO72_01725"
     CDS             <1..2198
                     /locus_tag="VFO72_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HET9478033.1"
                     /translation="VVAAGAPAPHPPSVHLTFGNPSNAVADLLQPNNYLMEKPSFALS
                     YNRDKGTPNWVSWHLETAWTGSFPRTDTFRADPAVSPDWYRVQSTDYFASGFDRGHMT
                     PNADRDNPASIPLNQETFLMSNMVPQAPNNNQGPWADLENFLRTLLSTNEVYVIAGPA
                     GVGGSGDNGPADTIAEGRVTVPAYTWKVALVLPTGDDDVSRVTAATRTIAVIIPNQNS
                     INSDWHTYLTTVDAVEALTGYDFFANVPEAVENAIEAGVDGNNPPGTENQSAFTNEDV
                     PVEITLNAVSANPSVTYTIVTSPANGVLSGVGPAFTFTPAANFHGADSFTFRVNDGVN
                     DSNTSTVSITINPVNDVPVADSQSVTTNSNTPVSITLTGSDFETAAGALIFEVTAGPT
                     HGTLSGSGAMLNYSPNADYSGLDSFKFTVRDTGDGSAAAVTSAEATVSITVNDALAPT
                     ITAPANVTVNTGAGATSCGAVVSDADLGNATAADNSGNVSVARTGVPSGNIFPVGTTT
                     VTYTATDDAGNSAQATQTVTVIDNTAPLLTAPAPTSANSGLSGQAAIPNVLASTVASD
                     NCGPVTLSQSPVAGTMVGVGTHTIEITATDAVGNTSTTTTTFTVNGGGLNFSLIAPET
                     ANRGKPAKVDAIFSNTTGEKLSVSFVVRYTGPCDTGIVNTGAVMINAGADRNVNVQFH
                     VPRDACTGLYTLTLEAHVNGVLVGTTTAETTVELATSMGVRSKRPTPQP"
     gene            complement(2404..2853)
                     /locus_tag="VFO72_01730"
     CDS             complement(2404..2853)
                     /locus_tag="VFO72_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9478034.1"
                     /translation="MSELSELYQQVILDHNKKPRNFRKLEHANHTAEGYNPLCGDHLT
                     VYLDVEGDAVKEIAFEGSGCAISKAAASMMTQAVKGKSREEAEELFTEFHSMVTGELD
                     EENEENSLGNLKIFAGVRDFPVRVKCATLPWHTLHAALNNKEHTSTE"
     gene            complement(2965..4206)
                     /locus_tag="VFO72_01735"
     CDS             complement(2965..4206)
                     /locus_tag="VFO72_01735"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET9478035.1"
                     /translation="MAPPVVTDSWDVQRIRADFPVLHQTVNGKPLVYLDNAASSQVPQ
                     MVIDRGSVYLEQEHSNIHRGVHYLSQRATTAYEGAREKVKRFINAREARECIFVRGAT
                     EGINLVMYGYGRKFIGPGDEIIISAMEHHANIVPWQMLCEEKGARLRVIPMNDAGELL
                     LDEYEGLLNERTKLVGVVHVSNALGTINPVKQMIGQAHKYGVPVLVDGAQAAPHMPVD
                     MQDLDCDFYVFSGHKMYAPTGSGIVYGKAELLEKMNPFQGGGDMIKTVTFEKTTYADL
                     PNKLEAGTPAIASQIGLGAAIDYLNSIGREQAAAHESELLRYATERISAIEGVRIIGT
                     AKNKASVVSFVIDDIHPHDIGTILDQEGIAIRAGHHCAQPVMQRFKVPATARASFAFY
                     NTKEEVDVLARTIERVIEIFS"
BASE COUNT          906 a         1257 c         1192 g          931 t
ORIGIN      
        1 ccgtagtggc tgcgggcgcg ccggcgccgc atccgcccag tgtccatttg acctttggta
       61 atccgtcgaa tgcagtcgcc gatcttctgc agccaaacaa ttacctgatg gagaagccgt
      121 cgttcgcgct ctcttacaac cgcgacaagg gcacgccgaa ttgggtcagt tggcatttgg
      181 aaactgcctg gaccggcagt tttccgcgca ccgatacgtt ccgcgcggac ccggctgtct
      241 cgcctgactg gtatcgcgtg cagtcaaccg attatttcgc ctccggcttc gatcgtggtc
      301 acatgacacc gaacgccgac cgcgacaatc cggcctcgat accgctcaat caggaaacgt
      361 tcctgatgtc gaacatggtg ccgcaagcgc ccaacaacaa tcaggggccg tgggcggatc
      421 tggaaaactt cctgcgcacc ctgctgtcaa cgaacgaggt ttatgtcatc gctggtcccg
      481 ccggtgtcgg tggttcaggt gacaacggtc cggcagatac tatcgccgag ggacgcgtaa
      541 ccgttccggc ttacacctgg aaggtcgcgc tggtgctgcc caccggcgac gatgacgttt
      601 cgcgtgtgac cgccgcgacg aggacaatcg ccgtgatcat acctaaccag aacagcatca
      661 acagcgactg gcatacttat ctcacgacgg ttgacgcagt cgaggccctc accggctacg
      721 acttctttgc gaacgtgccg gaggcggtgg aaaacgccat cgaggcaggc gtcgacggaa
      781 acaatccgcc gggaacagag aatcaatctg cattcaccaa tgaagatgta ccggtcgaga
      841 ttacgctcaa cgcagtgagc gccaatccgt cggttactta cacgatcgtt acttcaccgg
      901 cgaatggcgt gttgtccggt gttggaccgg cgtttacgtt tacaccggca gcgaacttcc
      961 acggcgcgga cagtttcacc ttccgagtca atgacggtgt taacgactcg aacacttcga
     1021 cggtcagcat cacgatcaac ccggtgaacg atgtgccggt ggctgacagt cagtcagtga
     1081 cgacgaacag caacacgccc gtttcgatta cgctgaccgg aagcgatttc gaaaccgcgg
     1141 ccggcgctct catcttcgag gtgacagccg gtccaacgca cggcacactc agcggcagcg
     1201 gcgccatgct gaattattcg cccaatgcag actacagcgg actagacagc ttcaagttca
     1261 ccgtccgcga tactggagac ggttctgctg cggccgtgac gagcgctgaa gcaaccgtct
     1321 cgatcactgt gaacgatgca ctggctccga ctatcacggc gcctgcgaac gtcacggtca
     1381 acaccggcgc cggtgctaca tcctgtggcg cggtcgtcag cgacgcagat cttggaaacg
     1441 cgaccgccgc cgacaactcg ggcaacgtga gtgtcgcacg caccggcgtt ccgtcgggga
     1501 acatctttcc ggtgggaacg acgacggtca cctacaccgc gactgatgac gcgggcaact
     1561 ccgcacaggc cacgcagact gtgactgtga tcgacaacac ggcgccgttg ttgactgctc
     1621 cagccccgac aagtgccaac tctggactca gcggccaggc ggctattccg aatgtgctcg
     1681 cgagcacagt cgcgagcgac aattgtggac cagtcacgct gagccagagt ccagtcgcag
     1741 gtacgatggt tggcgtcggc acgcacacga ttgagataac cgcgaccgat gcggtgggaa
     1801 acacaagcac cacgacaacg acgttcaccg ttaatggcgg aggtttgaac ttctcgctta
     1861 tcgcgcccga gacggcgaac cgtggtaagc cggcgaaggt cgatgcgatt ttcagcaata
     1921 ccaccggcga gaagttgtcc gtctcgtttg tggtgcgata cacgggccca tgcgataccg
     1981 gtattgtcaa tactggcgcc gtgatgatca acgccggtgc ggatcgaaac gtgaacgtgc
     2041 agttccatgt gcccagggat gcctgtaccg gcttgtacac gctgacactc gaggcgcatg
     2101 tgaacggagt gttggtgggg acaactacgg cggagacaac cgtcgagttg gccacgtcga
     2161 tgggggttcg gtcgaagcgc ccaacgccgc agccgtaacg cttctttgtg cagcctctgc
     2221 gtcgggctct tgtgctttgt ggtgaaaagt ttgtggggaa cgaataacta caaaagccac
     2281 agactacgca gaggctgcac aaagaagggc cacagatttg ccgattttcg attggcaatt
     2341 gccgattgaa aagcggcctt agttttcaaa tcgtcaattg gcaatcggaa atcggcaatg
     2401 tccctattcg gtagacgtgt gctctttgtt gttcagcgcg gcgtgcagtg tgtgccaggg
     2461 cagggtggcg catttcacgc gaacgggaaa gtcgcggacg ccggcaaaga tcttgaggtt
     2521 tccgaggctg ttctcttcat tctcttcatc gagttcaccc gtgaccatcg agtgaaactc
     2581 agtaaacaac tcttcggcct cttcccgact ctttcccttc accgcctgcg tcatcatgct
     2641 tgctgcggcc tttgaaatcg cgcagccgga accttcaaac gcaatctcct tcactgcgtc
     2701 accttcgacg tcgagataca cggtcagatg atcgccgcaa agggggttgt aaccttcggc
     2761 cgtatgattg gcgtgctcga gcttgcggaa gtttcgcggc ttcttgttgt ggtcgagaat
     2821 tacctgctgg tagagttcgc ttaattcgga catatggcat tttcaattgc cgatttttga
     2881 ttgccgattg tcgattgttc gctggtagcg atggctacct ttcgcgaagc tggcaatcaa
     2941 aaatcaacaa tcaacaatca aaaattaact aaagatctcg atcacgcgtt cgatggtacg
     3001 cgccagcacg tcgacctctt ccttcgtgtt gtaaaaagca aacgacgcgc gcgcggtcgc
     3061 cgggaccttg aaccgctgca tcaccggttg cgcgcagtgg tgcccggcac gaatggctat
     3121 tccttcctgg tccaaaatcg tgccgatgtc gtgcggatga atatcgtcaa tcacaaacga
     3181 cacgacgctc gctttgttct tcgccgtgcc gatgattcgc acgccttcga tcgccgaaat
     3241 gcgctcggtt gcgtaacgca acaactcact ttcatgcgcc gccgcctgct cgcggccaat
     3301 gctgttcaag taatctatgg ctgcacccaa tccgatttgc gacgcgatcg ccggcgtgcc
     3361 cgcttctaac ttattcggca aatcggcgta agtcgtcttt tcaaacgtga cggtcttgat
     3421 catatcgccg ccgccctgga acggattcat cttttccagc aactcagctt tgccgtacac
     3481 gatgccgctt ccggtaggcg cgtacatctt atgaccggag aaaacgtaga aatcgcagtc
     3541 aagatcctgc atgtcgaccg gcatgtgcgg cgccgcctgc gcgccatcaa ccagcaccgg
     3601 cacaccgtac ttgtgcgctt gaccaatcat ctgcttgacg ggattgatcg ttccgagcgc
     3661 gttcgagacg tggaccacgc ctacgagttt tgtccgttcg ttcaacaggc cttcatactc
     3721 gtcaagtaat aactcgcccg cgtcgttcat tggaatcaca cgcaaccgcg cacccttctc
     3781 ttcgcacagc atctgccagg gcacgatgtt ggcgtggtgc tccattgcgg agatgatgat
     3841 ctcgtcgcca ggaccaatga acttccgccc gtaaccgtac atcacgaggt tgatgccttc
     3901 agttgcgccg cgcacgaaga tacactcgcg cgcctcgcgc gcgttgatga accgcttcac
     3961 tttttcgcgc gcgccttcat aagcggtggt cgcccgctgg ctcaggtagt gcacaccgcg
     4021 atgaatgttt gagtgttcct gttcgagata gacgctgccg cgatcgatga ccatttgcgg
     4081 cacttgcgat gaggctgcgt tgtcgagata cacgagcggc ttgccgttga ctgtttgatg
     4141 caatacagga aagtcagcgc ggatacgctg cacgtcccag gaatccgtta ctacgggcgg
     4201 cgcgatggaa tcgacagttt gaacgatgcg actattcacg gctctccagt ttggtgttca
     4261 actgacgtag cactatttcg tccaat
//
LOCUS       DASTEY010000102         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1485503, whole genome shotgun sequence.
ACCESSION   DASTEY010000102 DASTEY010000000
VERSION     DASTEY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485503"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFO72_01740"
     CDS             <1..293
                     /locus_tag="VFO72_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9478036.1"
                     /translation="TYCATKSALLSLGQAMRAYLADSGVRVITVLPTTIDTDMSSAAD
                     VPKMTKEFVAAEILSAIREEAHDPPIGDEAKSILERFAKDPVGLEQMLMKYK"
     gene            378..1514
                     /locus_tag="VFO72_01745"
     CDS             378..1514
                     /locus_tag="VFO72_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9478037.1"
                     /translation="MNRRRNEFPTLASGIHLLSHSLGPVPRRVRQSMMAYCDAWEHHT
                     SEDAWATTWWQLSRRVGDRIARILGGAPASVQIQPNASIALATVASCFDFPSGRRSKV
                     VTTALDFPSMEYIWDAQRELGARVEVIKSNDGISVPLEKILQAIDDETCLVALAHTSY
                     RSSYRIDVPAVVERAHANGALVLLDVYQSAGVLPLHAADWKVDFLIGGTIKWLCGGPS
                     CGYLYVRPDIQKDLRPRLTGWVAHDAPFEFAAAPMRYARSVRRFAQGTPSIPALYSAI
                     PGLDIIESVGVTQIAAESQRRMQLMIDFARERGWTINTPLDVERRGGSVMIGVDDAKS
                     VSEQLAERRVFVDWRPGAGLRISPHFFNTDEEVEEALQILATLT"
     gene            1565..2050
                     /locus_tag="VFO72_01750"
     CDS             1565..2050
                     /locus_tag="VFO72_01750"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9478038.1"
                     /translation="MTNKLKLNEPAPDFTLKDGSGNDWRLSDHRGKVVVLLFYPGDET
                     PVCTRQMCSVRDRWDDYQATGAEVVGISSDSVESHRKFAEHHELPLRLLSDTEGKVST
                     LYGARSLIPGKVARSVFVIDADGVVRYQDVRPLGLLKPKDDVIISAIREAQRGPQSTG
                     R"
     gene            2057..2341
                     /locus_tag="VFO72_01755"
     CDS             2057..2341
                     /locus_tag="VFO72_01755"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET9478039.1"
                     /translation="MAETIKCAHCGEKRPALPFAPFPNELGQRIAAEICQPCWSGWLQ
                     KQTQIINHYGLDLTNPDAQNFLFDNIRGFLFGETPNLAEIDTTKEGTVKW"
     gene            2526..3041
                     /locus_tag="VFO72_01760"
     CDS             2526..3041
                     /locus_tag="VFO72_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9478040.1"
                     /translation="MRRRTTGLIALAALSMILPGCNGTANQNGNANANMNMNANMMAS
                     PTPTANANTGAETRRAPTREEYERDRERYQREAKESGRTIGTGLNDGWLWVKTRFDLA
                     AADDLRDSTINVDVDNGVVTLTGTVATAAQKARAEQVVKDNDGVRSVRNQLRVSASGD
                     ANANGNRNANR"
     gene            3318..>4938
                     /locus_tag="VFO72_01765"
     CDS             3318..>4938
                     /locus_tag="VFO72_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9478041.1"
                     /translation="MPKVRPLAVALFTLSLVTIVFVSFADAQRSAIDTYAITNARIVT
                     VSGPIIERGTVVIRNGLIAAVGPNVNAPPDARIVDGAGLTVYPGLIDSYTNLALPEAS
                     PSPSPAAGGGGGFFAAQPRPSPGGPNSTRPPGLQPEVMVEEVIRPGGTAIESSRSIGI
                     TTALTSPRTGIWMGQSALINLAGNTPQEMIVRSPVAMHVGFTPLRGTYPGSLMGVLAT
                     LRQMMLDAQRYRDSVQAYERSPRGMRRPDVDRSLAALVPVVDGRMPVVMLANSEREIA
                     RTLDLASEFKLRLIIAGGREADRLADRLVKQNVPVLLSLNLPRRTTPALPEADPEPLR
                     VLRERVEAQQTAGKLAKAGVRFAFQSGSLTNISDLLTNANRTIENGLSATDALRAFTI
                     WPATIFGVENQLGSIETGKIANLTVTRGDLFDRNSRVVHVFIDGRPVDLRPATGGPGS
                     RNVLSGAWNLTINLGQGERTATLNLQQEGERLSGSISGPFGAGEISNASTTTAGEVRF
                     TVPLQIEGQTREATFTGTFSGNEIRGTVTVVG"
BASE COUNT         1029 a         1410 c         1455 g         1044 t
ORIGIN      
        1 gcacctactg tgcgacgaag tcagccctgc tgtcgttggg ccaggcgatg cgcgcgtatc
       61 ttgcagactc cggtgtccgt gtgattacgg tgttgccgac gacgatcgac actgacatga
      121 gcagcgccgc ggatgttccg aagatgacga aggagtttgt cgcggcggag atactgtctg
      181 cgattcgcga agaagcgcac gacccgccga ttggcgatga agcaaagagc attctggagc
      241 ggtttgcgaa agatcccgtt ggcctggaac aaatgttaat gaagtacaag taaaatttcg
      301 aatttcgaat tgcgaattgc gaattttcag aaatatctac aattcgcaat ccgaaattcg
      361 caattcgcaa ttttcttatg aatcgacgac gcaacgaatt tcccaccctg gcctcgggca
      421 ttcatctgct cagtcacagc ctcggcccgg tgccgcgtcg cgtccggcag tcgatgatgg
      481 cttactgcga cgcctgggaa catcacacca gcgaagacgc gtgggccacc acctggtggc
      541 aactgtcgcg tcgagtcggc gatcgcatcg cacgcattct cggcggtgct cctgcttcgg
      601 ttcagattca accgaatgca tcgatcgccc tggccactgt cgcctcatgt tttgactttc
      661 ccagcggacg ccgttcgaag gtcgttacga ccgcacttga tttcccatcg atggaatata
      721 tctgggacgc gcagcgagag ttgggcgcgc gcgtagaggt cataaagtca aacgacggta
      781 tttcagttcc actcgaaaag atacttcagg cgattgatga cgaaacctgt ctcgttgccc
      841 tcgcgcacac ttcgtaccgc agttcgtatc gcatcgacgt tccggccgtc gttgagcgcg
      901 cgcacgcgaa cggcgcgctc gttttgttgg acgtgtatca gtctgccggt gtactgccgc
      961 tgcacgctgc tgactggaaa gtcgatttct tgattggcgg cacgatcaag tggctttgcg
     1021 gcggaccatc atgcggttat ctctatgtca ggccagacat tcaaaaagat cttcgcccgc
     1081 ggttgactgg ttgggtcgcg catgatgcgc cgtttgagtt cgccgctgcg ccaatgcgtt
     1141 acgcgcgttc cgtgcgccgg ttcgcacagg gaacaccgag tattccggcg ctttactcgg
     1201 cgatcccggg gttagacatc atcgagtcgg tcggcgttac gcagatcgcc gccgagtcac
     1261 aacgtagaat gcagttgatg atcgatttcg cgcgcgaacg tggttggacc atcaacacgc
     1321 cgcttgacgt ggaacgccgc ggtggttcgg taatgatcgg cgtggacgac gcgaagtccg
     1381 tgtcggaaca actggctgag cggcgagttt ttgttgattg gcgtccgggc gccggcctcc
     1441 gcatctcgcc ccacttcttc aacaccgacg aagaggtcga ggaagctttg cagatcctcg
     1501 caacactgac gtagtacagg catcttggct gacggtgaca gactagaaag tttgtcctac
     1561 tttcatgact aacaagctaa aactgaacga accggcgcca gatttcacgc tgaaagacgg
     1621 cagtggcaac gactggcgcc tgtcagatca tcgcggtaag gtggtggtgt tgctgttcta
     1681 tccgggagat gagacgccgg tctgcacgag gcaaatgtgc tcggtgcgcg atcgctggga
     1741 cgattatcag gcgaccggcg ctgaagtggt tggcatttcg tctgactcgg ttgaatcgca
     1801 tcgcaagttt gccgagcatc acgagcttcc gttgcgactg ctctcagaca ccgaaggaaa
     1861 agtatcgacg ctttacggcg cgcgatcgtt gatacccggc aaagttgcgc gatcggtttt
     1921 tgtgatcgat gctgatggcg ttgttcgtta ccaggacgtg cgcccgctgg ggctgctcaa
     1981 gccgaaggat gacgttataa tttcggcaat ccgcgaagca cagcgaggac cgcaaagtac
     2041 agggaggtga ggttccgtgg ctgaaactat caaatgcgca cattgcggcg agaagcgccc
     2101 cgcacttccg ttcgcgccgt ttccgaatga attaggacag cggatagccg cggagatctg
     2161 ccagccgtgc tggagtggat ggttgcaaaa gcaaacgcag attatcaatc actacggtct
     2221 ggatcttacc aaccccgacg cccagaactt cctgttcgac aacatcagag gttttctctt
     2281 cggcgagacc cctaacctcg ccgaaatcga cacgactaaa gagggaactg tcaaatggtg
     2341 atcggatcgc ggcccggcga ttgctacctt gtccattctg cctgactcgc ccgcaattgt
     2401 tttagacccc ccattagtga tcgagcacgt tcgagaagtg gttcgacgtg tgctctgcct
     2461 tatttccatg cgcgcgtatg ttttcagaac tagcgcgttc aatgaaggag gaggactttc
     2521 ccaagatgag acgaagaact acaggactca tagctttagc tgcgctttca atgatcctgc
     2581 cgggttgcaa tggaaccgct aaccagaacg gcaacgccaa cgcgaacatg aacatgaacg
     2641 ccaatatgat ggccagtccg acgccgaccg ccaacgcgaa tacgggggcg gaaacgcgcc
     2701 gcgcgccgac gcgcgaggag tatgaacgtg acagggaaag atatcagcgc gaggcgaaag
     2761 agtcaggccg gacgatcgga acaggtttga acgacggctg gctgtgggtg aagacgcgct
     2821 ttgatctggc tgcggctgat gacctgcgtg attcgaccat taatgttgac gttgataatg
     2881 gcgtcgtgac gctcacgggc acggtggcga ccgcagcgca aaaggctcgt gcggagcagg
     2941 tggtgaagga taacgacggc gtcaggtcgg tgagaaacca gttgagggtt tccgcgtcgg
     3001 gcgacgcgaa cgcgaacggc aacaggaacg cgaaccggta agttgcagcg tgcccgaagt
     3061 ggcctctgcc cgagtgacgc agcttgaggc agtagccgaa cttgagattt gggacgcctt
     3121 tttcctggga ccgcaggcgt ctcgcctgca acgcgtcgaa tcggacgctg ccgtgagatg
     3181 caggcgagac gcctgcggtc ccaggaaaga ggcgtcccaa acttccccct gcgcacttcc
     3241 cctggcggcc atttttaagc taagctctgg tctctttcct gctttcgcaa ttcaaactat
     3301 ctgggatttg gttgcctatg ccaaaagtcc gcccgcttgc cgtagcctta ttcacactct
     3361 ctctcgtcac gatcgttttc gtttccttcg ccgatgccca gcgcagcgcg atcgacacct
     3421 acgcgatcac taacgcacgc atcgtgaccg tctctggacc aataatcgaa cgcggcaccg
     3481 tcgttatccg caacggactg atcgcggccg tcggcccaaa cgtcaacgcg ccgcctgacg
     3541 cgcgcatcgt cgacggcgcc ggtctgacgg tctatccggg actgatcgat tcctatacga
     3601 atctcgcgct gcctgaagcc tcgccttcac catcgccggc cgcaggtggt ggtggaggtt
     3661 tcttcgctgc tcagccacgt ccgagcccgg gcggtccaaa ctcgactcga cctcccggct
     3721 tgcaacccga ggtcatggtc gaagaggtga ttcgtcccgg cggaacggcg atcgagtcct
     3781 caagaagtat cggcatcact accgcgctca cgtcaccacg cacgggaatc tggatgggcc
     3841 agtcggcact gatcaatctg gccggcaaca cgccgcaaga gatgatcgtt cgttctccgg
     3901 tcgcgatgca tgtcggtttt acgccgctgc gtggtaccta tccgggttcg ctgatgggcg
     3961 tcttggcgac cttgcgccag atgatgctcg atgcgcagcg ttatcgcgat tcggtgcagg
     4021 cgtacgaacg ttcgccgcgc ggtatgcgcc gtccggacgt agaccgatcg ctggcagcgt
     4081 tggtgcctgt ggttgacggc cgcatgcccg tggtgatgtt ggcaaatagt gaacgcgaga
     4141 ttgcccgcac actcgatctc gcctcggagt tcaagttgcg gctgatcatc gccggcggcc
     4201 gggaagcaga tcgactcgcc gatcgtttag tgaaacagaa cgtgccggtc ttgttgtcgt
     4261 tgaacctgcc gcggcgcacc acgcccgcgc ttccggaagc ggatcccgag ccgctacgcg
     4321 tgctgcgcga gcgggtcgaa gcccagcaaa ccgcgggcaa actcgcgaaa gccggagttc
     4381 gttttgcctt tcaatcaggt tcactgacga acatttccga tctgctaacc aacgcgaacc
     4441 gcacgattga gaacggtctc tccgcaactg acgcgttgcg cgccttcacg atctggcctg
     4501 cgacgatttt cggtgtggag aaccaactcg gatcgatcga gaccggcaag attgcgaatc
     4561 tcaccgtgac acgcggagat cttttcgatc gcaatagtcg cgttgttcac gtctttatcg
     4621 acggacgtcc ggtggacctc cgccccgcaa ctggtggtcc aggttcacgc aacgtgttga
     4681 gcggcgcctg gaacctgacc atcaaccttg gccagggcga aaggaccgcc acattgaatc
     4741 tgcaacagga aggcgagcgg ctgagcggtt cgatctccgg cccgtttggc gccggcgaga
     4801 tctcaaacgc ctccacgacc acagccggcg aggttcgctt cacagttcca ttgcagatcg
     4861 aaggacaaac aagagaagcg acgtttaccg gaactttttc aggcaacgaa attcgtggca
     4921 ctgtaaccgt cgtcggcc
//
LOCUS       DASTEY010000103         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_183527, whole genome shotgun sequence.
ACCESSION   DASTEY010000103 DASTEY010000000
VERSION     DASTEY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183527"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VFO72_01770"
     CDS             <1..1134
                     /locus_tag="VFO72_01770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9478042.1"
                     /translation="DRVRKGHLWIYRSDLVDVNAEGGSIVVVKDERGNFIGQALYSAA
                     SQIALRFLTQKNEEIDREWWRRRIRDAAARRNIPADTNAYRLVYSEGDLLPSLIVDRY
                     NEVLVLQTLSQGTEALKSMLAEILIEEFQPRAVVERNDARVRELEGLPLIAATVYGEE
                     PGELEILQHGLRFVVSPGEGQKTGSFLDQRENRLAARAASETTERRRALDCFTFNGGF
                     ALHLASVCEEVIGVEISADAVAAAKQNAQLNTLTNVEFREANVFDYLRELEAGGERFD
                     TIVLDPPAFAKNRASLKSAVRGYKEINLRALKLLESGGVLITCTCSYHVAEDLFLEIV
                     GQAALDAHRRVQVIEKRMQASDHPVLLGMPETYYLKCVIARVL"
     gene            complement(1153..>3487)
                     /locus_tag="VFO72_01775"
     CDS             complement(1153..>3487)
                     /locus_tag="VFO72_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478043.1"
                     /translation="YQRRRTNFGFNGGGPGSGIWKTIDAGKTWRKLEGGGLPEGILGR
                     IGLDVSRSNPNVIYAQMEVGPSMGTGGEDPQAAQAAASPSPSPSPAASAAPSPSPAAS
                     PSPSPPNPKRSGVWRSDDRGRTWRIVSNENNRPMYYSQIRVDPKNPETVYVGGLNFSK
                     STDGGKTFRSLQGGLAHVDNHAIWINPANSDHLLVGNDGGLNVSYDQGATWEFINTIP
                     AAQFYAIGVDMRKPYYVYGGLQDNGSWGGPSQTRNQQAGITNADWYRVGGGDGFYTQI
                     DLTDHNIIYVESQGGNMRRLDLRTGSSVSIRPRGVPRRGGRGGQGGAQPAASPSPEAS
                     PSPDTAAQLAAFAAQQGIGGFGGGNLTSNVVPTPPPNEVYRFYWNTPIHLSPHNPRVV
                     WAGGNRLFRSLDRGDTWTKTIDLSKQIDRNTLSIMGVPGNQPMASKHDGYTGYGYVVT
                     VAESPNVPGIVWAGTDDGNVQVSRDGGATWTNVSKNVPGIGDTYHISRVEPSRFDAGT
                     CYLAVDGHRLDDMKPYLFVTRDYGATWKSIVNNLPKWGNVNVVREDPKNKDLLYVGTE
                     FGLYISLNGGAEWKPFMSGMPVIRVDDILVHPRDNDLIVGTHGRGIFILDDITPLQQL
                     SQKVMDSEAHLFEVRPGMMWLNDPRLARYWGGGKLFRGANPAPGTAINYYLKSAPSGD
                     VKIAISDYTGKVVRNITGTKDVGLNRIQWNLRSDPPPRPSTPQSGRGGGGGGGRGFGN
                     IFTQGLPLEAGTYNLKLTVNGKDYTTKVVLENDPGFN"
BASE COUNT          699 a         1008 c         1027 g          753 t
ORIGIN      
        1 gatcgcgtgc ggaagggcca tctgtggatc tatcgcagcg accttgtgga cgtgaacgcc
       61 gaaggcggtt cgattgtagt agtcaaagac gagcgcggca atttcatcgg ccaggctttg
      121 tacagcgctg cctcacagat tgcactccgt tttctgacgc aaaaaaacga agagatcgat
      181 cgcgagtggt ggcgtcgccg gatacgcgac gcggcggccc ggcgaaacat tcccgctgac
      241 accaatgcct accgtttggt ctactctgaa ggtgatttgc tgccgtcgct gatcgtcgat
      301 cgttacaacg aagtactggt gttgcagacg ttgtcgcagg gaaccgaagc tctgaaatcg
      361 atgcttgcgg agatccttat cgaggagttt caaccgcgtg ctgtggttga gcgcaacgac
      421 gctcgcgtgc gcgaactcga aggattgccg ttgatcgccg ccactgttta tggcgaagag
      481 cccggcgaac tggagattct tcagcacgga ttgcgttttg ttgtttcgcc aggcgagggg
      541 caaaaaaccg gatcgtttct ggatcagcgt gaaaacagac ttgcggcacg cgcagcgtcg
      601 gaaaccaccg agcggcggcg cgcactcgac tgctttacgt tcaacggcgg tttcgcactg
      661 catctcgcga gcgtttgtga agaggtaata ggcgttgaga tttcggcgga tgcggtcgcc
      721 gctgcgaaac agaatgctca gcttaacact ctgacgaacg tcgagtttcg tgaggcgaac
      781 gttttcgact atttgcggga actcgaagcg ggtggcgagc gcttcgacac gatcgtgctt
      841 gatccgccgg cgttcgcgaa gaaccgcgcc agtctgaagt ctgccgtgcg tggttacaaa
      901 gaaattaact tacgcgcgtt gaagttgctc gaaagcggag gtgtgctgat tacttgcacg
      961 tgttcgtatc atgttgctga ggatctgttt ctggaaatcg tgggccaggc cgcgctggat
     1021 gcgcaccggc gcgtgcaagt catcgaaaaa cgcatgcagg cctcggatca tcccgtgttg
     1081 cttgggatgc ccgagaccta ctatttgaaa tgcgtaatcg cgcgtgtgct ttgagtcgcg
     1141 gattatacgg atttagttaa accctgggtc gttttcgaga acgactttcg tcgtgtagtc
     1201 cttgccgttg accgtcagct tcagattgta ggtgccggct tccagcggca ggccctgagt
     1261 aaagatgttg ccgaagccgc ggccaccgcc accgccgccg ccacggccag actgcggagt
     1321 gctcggacgc gggggtggat cgctgcgcaa gttccactga atcctgttca gaccaacatc
     1381 tttcgtgccg gtgatgttgc gcacaacctt acccgtgtaa tccgaaatcg cgatcttcac
     1441 gtcacccgac ggcgcagact tcaggtaata attgatggcg gtgcccggag caggattggc
     1501 gccacggaag agtttcccac cgccccaata acgagccagc cgcggatcgt tcagccacat
     1561 cattccggga cgcacttcga agagatgcgc ctctgagtcc atcaccttct gcgacaactg
     1621 ctgcaacggc gtgatgtcgt ccaaaatgaa gatgccgcgc ccgtgagtgc cgacgatcag
     1681 atcgttatcg cgcgggtgga ccaaaatgtc gtcgacgcga atcaccggca tccctgacat
     1741 aaacggcttc cactcagcgc cgccattcag cgagatgtac agcccaaact cagtgccgac
     1801 gtacagcaga tccttgttct tcggatcttc ccgcacgaca ttgacgttac cccacttcgg
     1861 caggttgttg acgatcgact tccacgttgc gccgtaatcg cgcgtgacga agagatacgg
     1921 cttcatgtca tccaaacgat gcccatcgac cgcgagataa cacgtgccgg catcgaagcg
     1981 cgacggctca acgcgcgaga tgtgataagt gtcgccgatt cccggcacgt tcttcgacac
     2041 gttggtccag gtggcgccac cgtcacgact gacctgtacg ttgccgtcgt cagtgccggc
     2101 ccaaacaatg cccggaacgt tcggcgactc cgcaaccgtt acgacatagc cgtaaccggt
     2161 gtaaccatcg tgcttggagg ccattggctg gtttccgggc acgcccatga tcgacaaagt
     2221 gttgcgatcg atttgtttgc tgagatcgat tgttttggtc caggtgtcac cacggtccag
     2281 cgatctgaac aggcgattgc cgcccgccca aacaactcgc gggttgtgtg gcgagagatg
     2341 aatgggcgtg ttccagtaga agcggtagac ctcgtttggt ggcggtgtag gtacgacgtt
     2401 cgacgtcaag ttccctccgc caaaaccacc gatcccctgc tgggcagcga atgcagcgag
     2461 ctgagcggcc gtgtccggac tcggcgacgc ctcaggtgaa ggactcgcag caggttgggc
     2521 gccgccttgc ccacctcttc ctccacgtcg cggcacgccg cgcgggcgaa tactcacact
     2581 gcttccggtg cgcagatcaa gacgccgcat attaccgccc tgcgattcca cgtagataat
     2641 gttgtggtcc gtcagatcga tctgcgtgta gaaaccatca ccgccgccca cgcgatacca
     2701 atcggcgttc gtaatgcccg cttgttgatt tcgcgtttgg cttggtccac cccacgatcc
     2761 gttgtcctgt agaccaccat aaacgtagta cggcttacgc atgtcgacac cgatcgcata
     2821 gaactgcgcg gccggaatcg tattgatgaa ctcccacgtc gcgccctggt cgtagctgac
     2881 gtttagaccg ccatcgttgc cgacaagcag gtgatcgctg ttcgcgggat tgatccagat
     2941 cgcgtgattg tccacgtgcg ccaggccgcc ctggagcgaa cggaacgtct taccgccgtc
     3001 agtggacttg ctgaaattca aaccgccgac ataaaccgtt tcggggttct tcggatcgac
     3061 gcgaatctgg ctgtaataca taggccgatt gttttcgttg ctgacgatac gccaggttcg
     3121 tcccctgtcg tcggaccgcc aaacgcccga tcgtttcgga ttgggaggcg acggcgaggg
     3181 ggaagcggcc ggactcggtg aaggcgccgc gctggcggcc ggcgacggcg agggacttgg
     3241 actcgccgcc gcctgagcgg cttgcggatc ctcaccgccg gttcccatgc ttggtccaac
     3301 ttccatctgc gcgtaaatga cattcgggtt cgaacgggag acatccaaac cgatgcggcc
     3361 gagtattcct tcgggtaagc cgccgccttc gagtttcctc caggtcttgc cggcgtcgat
     3421 agtcttccag atgccgcttc ctggtccacc gccattgaaa ccaaagttag tgcgacggcg
     3481 ctggtag
//
LOCUS       DASTEY010000104         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_216605, whole genome shotgun sequence.
ACCESSION   DASTEY010000104 DASTEY010000000
VERSION     DASTEY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216605"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>2084
                     /locus_tag="VFO72_01780"
     CDS             14..>2084
                     /locus_tag="VFO72_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478044.1"
                     /translation="MTSAIQGNGYQVEFSFENPTIPTGDGGGAGSGGGGIVEFISEDI
                     LKTSEPILHETPGTFRVTLAHDERPHRSQAPLFYQDKKRTFFVVPHGKYTGGGGGGGG
                     GIDELTVGLNTAPLELPDILSHQAAIALAANNNGPVATRALGGGRIGNILAPQVSLAR
                     SFQPLHWEAKHFRFDNFYHPFISLLIEQLNRFGIDGVLRPDPDRESGERRAVIAKLRR
                     QRNRKVFFNSTYKPNDNIVLNVLDMSAGQKQQAGPMDIFDFAYGTAYSIYNWELFFHA
                     PFMIAKRLGANQRFSEAHRWFQYIFDPTDNSAAEQWPERVWQIKPFFEHGQGKSIDRA
                     MLLLKSSNLSHAEIEERQSLRDQIEAWRKSPFNPHLIARMRNEAYMKSIVMAYLDNLI
                     AWGDQLFRQDTRESVNEATQLYLLAAEILGERPQEIPAHEGVRKTINGVEVNTFNQLR
                     GRLDAFSNALIDLETMIYPTNNQGGGGIKGLLGPQNLKLAPPDNDDDLPLAIEPPDNG
                     FVKDLPLATPVPAILGPTLFFCIPKNDKLLGYWDTIADRLFKIRHCLNIEGVARQLAL
                     FAPPIDPGLLVKAVAAGLDIGSVLSDLSAPLPHYRFHVLVQKANEVISDVKGLGAMLL
                     ATLEKKDAEELALLRSTHETQILEAMRQVKLRQIEEAKAAKEALEASKRALEERRTFY
                     ETREEKSF"
BASE COUNT          488 a          589 c          580 g          427 t
ORIGIN      
        1 aaagttccga ctggtgactt cagccattca ggggaacggc taccaggtcg aatttagttt
       61 tgagaacccg actattccca ctggcgacgg tggtggcgca ggctcaggcg gaggaggaat
      121 cgttgagttc atatctgagg acattctgaa gacgtcggag ccgattctcc acgaaactcc
      181 aggaacattt cgagtgacat tggcgcatga cgagcgcccg catcgttcgc aggcaccgct
      241 tttttatcag gataagaagc gcacgttttt tgtcgttccg cacggcaagt acacgggcgg
      301 tggcggtggc ggtggcggcg gaatcgatga attaacagtc ggtctcaata ccgcgccgct
      361 cgagttacct gacatccttt cgcaccaggc agccatcgca ctcgctgcaa acaataatgg
      421 tccagttgcc actcgcgcac tcggcggcgg gcgcatcggc aacattcttg cgcctcaagt
      481 gagtctcgcg cgcagctttc agccgctgca ctgggaggcg aaacatttcc ggttcgataa
      541 cttctatcat ccgtttatca gtttgctgat cgaacaactc aaccgcttcg gaatcgacgg
      601 agtgctgcgg cctgatcccg atcgagagtc gggcgagcgg cgtgctgtca tcgcaaagct
      661 gcggcgccag cgaaatcgca aggtgttttt caactccacc tacaaaccga atgacaacat
      721 cgtgctcaac gtcctcgata tgagtgccgg ccagaaacaa caggccggcc cgatggacat
      781 tttcgacttc gcttacggta ccgcgtattc aatctacaac tgggagctct tcttccatgc
      841 gcctttcatg atcgccaagc gactcggcgc gaaccagcgc ttctccgagg ctcacagatg
      901 gtttcaatac atcttcgatc cgaccgacaa ctcggccgcc gagcaatggc ccgagcgcgt
      961 ctggcaaatc aaaccattct ttgagcacgg tcagggcaaa tcgatcgatc gggcaatgct
     1021 gttgctcaaa tcgagcaatc tcagtcacgc ggaaatagag gaacgccagt cactgcgtga
     1081 ccagatcgag gcgtggcgca agtcgccttt caatccgcac ctgatcgcgc gcatgcggaa
     1141 cgaagcgtac atgaagagca tcgtgatggc ttatctcgac aatctgatcg cctggggcga
     1201 tcaacttttc cgccaggaca cgcgcgagtc agtcaacgag gccacgcaac tatacctgct
     1261 ggcagcggag attctgggcg agcgtccgca ggagattcca gcgcatgaag gggttcgaaa
     1321 aacgattaac ggcgtggagg tcaatacgtt caatcaactc cgcggccggc tcgacgcttt
     1381 ctcaaacgcg ctgatcgatc tcgaaacgat gatttacccg acgaataacc agggaggcgg
     1441 tgggatcaaa ggtttgcttg gtccacaaaa cttgaagctg gctcctcctg acaacgatga
     1501 cgatcttccg ctcgcaattg agcctccgga caatggattc gtgaaggacc tgccactcgc
     1561 gacaccggta cccgcgattc tgggtccgac tctgttcttc tgcattccaa agaacgacaa
     1621 gctgctggga tactgggaca cgattgccga ccggctgttc aagattcgcc actgcctgaa
     1681 catcgaaggc gtcgcacgac aactcgcgtt gttcgcgccg ccgatcgatc cgggcttgct
     1741 ggtgaaagcc gtggctgccg gactcgacat cggttctgtg ttgagcgacc tcagcgcacc
     1801 actccctcac tatcgcttcc acgtgctggt gcagaaggca aacgaagtaa tcagcgacgt
     1861 gaagggcctc ggcgctatgc tgctggcgac tttggaaaag aaagatgctg aagaacttgc
     1921 tctcctgcga tcgactcacg agacgcagat ccttgaagcg atgcgccagg taaagctgcg
     1981 tcagattgaa gaagcgaagg ctgcgaagga agctctcgaa gcgagcaagc gtgccctgga
     2041 agaaagacgc acgttttacg aaacgcgtga agagaagagc ttca
//
LOCUS       DASTEY010000105         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_842907, whole genome shotgun sequence.
ACCESSION   DASTEY010000105 DASTEY010000000
VERSION     DASTEY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842907"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFO72_01785"
     CDS             <1..479
                     /locus_tag="VFO72_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478045.1"
                     /translation="GFEPLDILHGIWMVLKQIAEGRTEIENQYKRVVPGAGNPAALDA
                     IGKVFELREFFEWRGLGSIDHSGVRIRDEYAEFDAERKFSVPALKIADPKSCQCGEVL
                     KGVIKPWDCKVFGRDCTPETPLGALMVSSEGACAAYYQYGDIEKIHDTRALVEEMA"
     gene            476..1558
                     /gene="hypE"
                     /locus_tag="VFO72_01790"
     CDS             476..1558
                     /gene="hypE"
                     /locus_tag="VFO72_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET9478046.1"
                     /translation="MSSVVELATKNGKPRHRGKVKVPHVTLAHGGGGKAMHDLIDDIF
                     VEMFDSEGQAPLEDQARLGISSLAALGDRLAFTTDSYVVKPLDFPGGNIGKLAICGTV
                     NDLAVGGAIPLYLSCSVIIEEGLPVDTLRMVARTMAETARDAGVKVVTGDTKVVERGA
                     CDRLFINTAGVGVIREGVDLGVDKCQPGDAILVNGLLGDHGAAILNARGDMQLSTPIE
                     SDCASLHGLIDALLSAVPRTRFLRDATRGGLATVLNEIAEASQVGIEIYEDRTPIRSE
                     VKGFCEILGLDPLYLANEGKIVCVVPAEDAETALATLKSHPLGKEAAKIGEVNDHRAG
                     RVVMQTVFGGRRIVDMLVGEQLPRIC"
     gene            1653..2276
                     /locus_tag="VFO72_01795"
     CDS             1653..2276
                     /locus_tag="VFO72_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478047.1"
                     /translation="MSSTNEIKAAEGVTIRIKDKDQVLELDYDGAVCQHPGSLWWGTA
                     VGFRAMQVAAQALSKDGLWSRDNLYIVTGHPGPGVKDTIDYITGAPSRDRYKMIVADG
                     CDMKCNSKMKYEWWVSDGEKTANINLRPDFVPREFYELSDRLETNETTKDDIKAFRIF
                     KVNLSTRIWNAPLEENFRVQIIDKPLQPGEVPDAAKAQDYFDLPYAD"
     gene            2419..3141
                     /locus_tag="VFO72_01800"
     CDS             2419..3141
                     /locus_tag="VFO72_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9478048.1"
                     /translation="MVSGAAGYAHRLEQQMLKQREFEHSWIRKPSVYEFGTEDDLFYF
                     TMEYVNGVQLSTFISRNTVRDVDPLIDQLLTFVRSNICEVTADFSTAISEKLWRISST
                     SRFQIDRYVQYCMAFDWRAIPTGYCHGDLSFENILVHKSNLYFIDFLDSFIETPYLDL
                     SKLLLDILVLWSWRFENRRPIIKNTHIYDIVLSQLKSREVEIVRRFLVLNLLQILPYA
                     NAHHNSLFVQDALKHLAVKFNI"
BASE COUNT          736 a          855 c          908 g          687 t
ORIGIN      
        1 cgggtttcga gccgctcgat atcctgcacg gcatttggat ggtgctcaaa cagatcgccg
       61 aaggtcgcac ggagatcgag aaccagtaca aacgcgtggt tcccggcgcc ggcaaccccg
      121 cggcactgga cgcgatcggc aaggtcttcg agttgagaga gttcttcgaa tggcgtggtc
      181 tgggatcgat cgatcattca ggcgtgcgca tccgtgatga gtacgcggag tttgacgcag
      241 agcgaaaatt ctccgtgccg gcgctcaaga ttgccgatcc gaaatcctgc cagtgcggcg
      301 aagtgctgaa gggcgtgatc aagccgtggg actgcaaagt cttcggtaga gattgcactc
      361 cggaaactcc gctcggcgcg ctgatggtct catcggaagg cgcctgcgct gcctattatc
      421 aatacggcga catcgagaag attcacgata ccagagcatt agtcgaggag atggcatgag
      481 tagcgttgta gagttagcaa caaagaacgg aaagcccagg caccgcggca aggtgaaggt
      541 gccacacgtc acgttggccc acggcggcgg cggcaaggcg atgcacgacc tgatcgacga
      601 catcttcgtc gagatgttcg atagcgaagg tcaggcgccg ctcgaagatc aagcacgact
      661 tggcatatcg agcctggcgg cactgggcga tcgacttgcc ttcactactg actcctacgt
      721 cgtcaagcca ctcgatttcc ccggcggcaa catcggcaag ctcgcaatct gcggcactgt
      781 taacgacctc gctgtcggtg gtgcgatacc gctctacttg tcgtgctccg tcatcattga
      841 ggaaggcctg cctgtcgaca cactgcgcat ggtcgcgcgg accatggcgg agacagctcg
      901 ggacgctggc gtaaaagtgg ttaccggcga tacgaaagtt gtcgaacgcg gagcctgcga
      961 caggttgttt atcaacactg ccggcgtggg tgtcattcgt gagggcgtcg atctcggtgt
     1021 cgacaaatgc caacccggag acgcgatcct cgtcaacggc cttttgggcg accacggcgc
     1081 tgcgattctc aatgctcgtg gtgacatgca gttgagcacg ccgatcgaga gtgattgtgc
     1141 ttccctgcat ggtctgatcg atgcgttgtt gtcagccgtc ccgcgtacac gattcctgcg
     1201 cgacgcgacg cgtggcgggc tcgccacggt cttgaacgaa atcgccgagg cgtcgcaagt
     1261 cgggatcgag atctacgaag accgcacgcc gattcgcagt gaggttaaag gcttctgcga
     1321 gatcctcggt ctggatccgc tctatctggc gaacgaaggc aagatcgtct gtgtcgttcc
     1381 ggcggaagat gctgagactg cactcgcgac actcaagtcg caccctctcg gcaaagaagc
     1441 cgcgaagatc ggcgaggtga acgatcatcg cgccggtcgt gtcgtgatgc agactgtttt
     1501 tggcgggcgc cgcatcgtcg acatgctcgt cggcgaacaa ctaccacgga tttgttagcc
     1561 acaaagaagc acaaaatgaa cagaggcaat cgttctgtgc tttttgtgcc ttttcgtggc
     1621 taaagacttt tgaggcagcg aaggagaatc gaatgtcaag caccaacgaa atcaaagcgg
     1681 ccgaaggcgt caccattcgc atcaaggaca aggaccaggt gcttgagttg gattacgacg
     1741 gcgccgtctg tcagcatccc ggatcgctgt ggtggggaac cgcggttggt ttccgggcga
     1801 tgcaagtggc tgctcaggct ctctcgaagg acggtctgtg gtctcgcgac aatctctaca
     1861 tcgtcaccgg tcatcccggc cccggcgtga aagatacgat cgactacatt accggcgcgc
     1921 ccagccgcga tcgctacaag atgatcgtcg ccgacgggtg cgacatgaag tgcaacagca
     1981 agatgaagta tgagtggtgg gtcagcgacg gcgagaagac tgcgaacatc aacctgcgac
     2041 ctgacttcgt gccgcgcgaa ttctacgagc tctccgatcg tctcgagacc aacgagacca
     2101 cgaaggatga tatcaaagcg tttcgtatct tcaaggtaaa cctctccacg cgtatctgga
     2161 acgcgccgct ggaagaaaac ttccgcgtcc agatcatcga caagccactg caaccaggag
     2221 aggtgcctga cgcagcaaaa gcccaggact acttcgatct gccgtacgca gattgagaag
     2281 gctaaagccg tggccgctct atcgccgatg caaactctcg ccgtaaagcc gccccaacaa
     2341 tataagctga aggaactgaa gggccattcc ggctgtcatg tgtttctctg tgaacgcgga
     2401 aatacaaggt ttgtcaaaat ggtatcggga gctgcaggtt acgcccaccg gctcgagcaa
     2461 cagatgctca agcagcgtga gtttgagcat tcctggatca ggaagccttc ggtctacgaa
     2521 ttcggcacgg aagacgatct tttctacttc acgatggagt atgttaacgg agttcagcta
     2581 agcactttca tctcacgcaa tacagttcga gatgtcgatc cgttaataga tcagctactc
     2641 acctttgtaa gatcgaatat ctgcgaagtg acagcagact ttagtactgc catcagcgaa
     2701 aagctgtgga ggatctcgtc gaccagccgt ttccagattg atagatatgt tcaatactgc
     2761 atggcattcg attggagagc cataccaaca gggtactgtc acggcgatct ctcctttgag
     2821 aacatactgg tacataagtc gaatctttat ttcatcgact ttctcgactc gttcattgaa
     2881 acgccatatc tggatctgag caagctactg ctggacattc tggtgttgtg gtcctggcgt
     2941 tttgagaacc ggcgcccgat catcaagaac acacatatct atgacattgt tctgtcgcaa
     3001 ttgaagagtc gcgaagttga aatcgtgcgg cgatttctgg tcttgaatct gctccagatc
     3061 ttgccatacg cgaatgctca tcataactcc ttattcgtgc aggacgccct caagcatctg
     3121 gccgtgaaat ttaatatatg agaacgctgc ttgtacccgc ggcgggtcgc tccagccgct
     3181 atcctg
//
LOCUS       DASTEY010000106         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_760489, whole genome shotgun sequence.
ACCESSION   DASTEY010000106 DASTEY010000000
VERSION     DASTEY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760489"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VFO72_01805"
     CDS             complement(<1..1536)
                     /locus_tag="VFO72_01805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9478049.1"
                     /translation="MFLLQANTTSLINIADLQLIVPELILTVCACVALVMEVILPYRK
                     SKLTAYFSLTAIALAFISLALQWWYMRNALPFDGFYGMVRIDGFALLFKGIFLVAAGL
                     AIGISTRYLDIEGEQHGEYYALVLFATVGMMFIACGYDLISLYISLELMALTFYILVA
                     FTKREKRSNEAAMKYFLLGAFSSGVLLYGMSLLYGIAGSTNVGVIGQELGQLVPQIQG
                     ALQAATQGAGFVDDIAKLRPLLLLSMIALAAGLFFKVAAVPFHMWAPDVYEGAPTSVT
                     AFLSTGSKAASFALYARIFSEALGPMRADWAPLLGLVAAITIMVGNWAAVTQENSKRL
                     LAYSSISNAGYLLLGLVANNLYGYIGLIVYLFVYTLMNMGAFGIVISLRRRGIMGDNV
                     DDMTGLGEKAPGMAAMMTIFMLSLGGLPMTGGFIGKYFLFGGLVQRGTGPEGKSWYIW
                     LAIWAILNTVVSFYYYFRFIKVMYLGDRLADNRPLALSPALQTALIISVIGVLVVGVY
                     PHPL"
     gene            complement(1543..>2301)
                     /locus_tag="VFO72_01810"
     CDS             complement(1543..>2301)
                     /locus_tag="VFO72_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9478050.1"
                     /translation="DQSTWVTSFGTFGVRSVMVFLAIVSIIYGALAAMYFVVKRDGDV
                     KKLVAYSSVSSMGMVMLGLFALNPNGVNGGVLQMINHGIYSGALFLLVGIIYERRHTR
                     NVSEFGGLSHVMPGYAAIFLGMVMTAIGLPLLAVFISEFLALRGAFEANPYWSAWAAL
                     GIILNAGYFLWLYQRMFFGNIDNPKNSKLPDLNVREWAYMVPLMILSLWIGVYPKPFL
                     EYIQQPVNAIVKQVRPDYQIPGTAAPAPTERAER"
BASE COUNT          594 a          681 c          608 g          418 t
ORIGIN      
        1 cagcggatgc ggataaaccc caaccacgag cactccgatc actgagatga tgagcgccgt
       61 ttgcagcgcg ggcgaaagcg ccagcggcct attgtcggcg aggcgatcgc cgagatacat
      121 cactttgatg aaccggaagt agtagtagaa agagaccacc gtgttgagga tggcccaaat
      181 agccagccag atgtaccaac tctttccttc cggtccggtg ccgcgttgaa ccagaccgcc
      241 aaacaggaaa tacttcccga tgaacccgcc ggtcatcggc aaaccgccga gcgacaacat
      301 aaagatcgtc atcatcgccg ccatcccggg agctttctcg ccgaggcccg tcatgtcgtc
      361 gacgttgtcg cccatgatgc cgcgccggcg caggctgatg acgatcccaa acgcgcccat
      421 attcatgagc gtgtagacaa acagataaac gatcaacccg atgtagccgt agagattgtt
      481 ggcgaccaac ccgagcagca aatagcccgc gtttgagatg gaagaatacg cgagcagtcg
      541 cttcgaattc tcctgcgtga ctgcggccca gttgccgacc atgatcgtga tcgccgccac
      601 cagtccaagt agtggcgccc aatcagcacg cattggacca agagcttcgc tgaagatacg
      661 cgcgtacaaa gcaaaactcg cggctttcga accggtcgac aagaacgccg tgaccgaggt
      721 cggcgcgcct tcgtaaacat ccggcgccca catgtgaaac ggcaccgccg caactttgaa
      781 gaacaacccc gcggccagcg caatcatgct caacagcaac aacggccgca gcttcgcgat
      841 gtcgtctaca aaaccggcgc cctgagtcgc cgcctgcaac gcgccttgaa tctgcggcac
      901 caactggccg agctcctggc cgatgactcc gacgttagtc gaacccgcaa tcccgtagag
      961 cagagacatt ccataaagca agacgcccga tgaaaacgca ccgagcaaaa agtacttcat
     1021 cgccgcttcg ttcgaacgct tttcacgttt cgtgaacgca accaggatgt agaacgtcag
     1081 cgccatcagc tcgagactga tgtagagcga gatgagatcg taaccgcagg caatgaacat
     1141 catgccaaca gtcgcaaaga gcaccagcgc gtagtattcg ccgtgctgtt cgccttctat
     1201 atcgaggtat cgcgtcgaga tgccgatcgc gagtccggcg gcgacgagaa agatgccctt
     1261 gaaaagcagt gcaaagccgt cgatgcggac catgccgtag aaaccgtcga acggcagcgc
     1321 gtttctcatg taccaccatt gcaacgcgag cgaaataaag gcgagtgcga ttgcggtcag
     1381 cgagaagtaa gcagtgagtt tgctcttgcg atacggaagt atgacttcca tcacgagcgc
     1441 cacgcacgcg caaacagtca ggatcaactc cggcacgatc agttgcaaat ccgcgatgtt
     1501 gattaaagac gtggtgttgg cttgcaagag aaacattttg gcttatcgct ccgcgcgttc
     1561 tgtcggcgct ggcgcggcgg tccccgggat ctggtagtcc ggtctgacct gcttcacaat
     1621 cgcgttcacc ggttgctgga tgtattcaag aaacggtttc gggtagacac cgatccaaag
     1681 cgacagaatc atgagcggca ccatgtaggc ccactcgcga acgttcaggt ccggcagctt
     1741 tgagttcttc gggttatcga tgttgccgaa aaacattcgc tggtagagcc acaggaaata
     1801 acctgcgttg agaatgatgc cgagtgcggc ccaggcggac caatatggat tcgcttcaaa
     1861 cgcaccgcgt agcgccaaaa actccgagat gaacaccgcc agcaacggca gaccaatcgc
     1921 agtcataacc atgcccagaa agatcgcggc gtaacccggc atcacgtgcg atagtccacc
     1981 aaactctgag acgtttcgcg tgtggcgtcg ttcgtaaatg attccgacca gcaggaacag
     2041 cgcgccggag tagatgccgt gattgatcat ctgcaacacg ccgccgttga cgccgttcgg
     2101 attaagagcg aacaacccca gcatcaccat acccatcgac gacaccgacg aataagcgac
     2161 aagcttcttc acgtcgccgt ctcttttaac cacgaaatac attgccgcca acgcaccgta
     2221 gatgatgctg acaatcgcaa gaaaaaccat cacactgcga acaccgaaag tgccaaagct
     2281 tgtgacccat gtgctttgat c
//
LOCUS       DASTEY010000107         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1419876, whole genome shotgun sequence.
ACCESSION   DASTEY010000107 DASTEY010000000
VERSION     DASTEY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419876"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFO72_01815"
     CDS             <1..451
                     /locus_tag="VFO72_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478051.1"
                     /translation="YWLPPSRWAGFPSRNLILPVLTLSALQMAYIARLTRAGMLEVLR
                     SDYIRTARAKGLSERDVVLKHALRGGLMPVISYTGPALAFLLTGTVVVEKIFALPGLG
                     NYFIQASLNRDEPLIIGIVAFIAITLLLMNLLVDIAYAYLDPRVRYQ"
     gene            458..1456
                     /locus_tag="VFO72_01820"
     CDS             458..1456
                     /locus_tag="VFO72_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478052.1"
                     /translation="MSTEKDPDQIHTPPEMAIAEADVEAGEFVRGSSLWRDAWRRLLK
                     NKLAVFGLIVVVIVTIASVLGPTIIKNTFGFTPDYIPSHDVKLSRSYPPFTGPNGEFS
                     WLHPMGTDNAGRDQLARVLQGGQISLFVGVISTLVSLLIGVTYGAIAGYVGGRLDNVM
                     MRIVDVLYSLPYVIVVIVLLSMFRSQTPRGQLILLFIALGSISWLTMARIVRGQVLSL
                     KNQEFVLAARATGVSATRIIFRHLVPNTLGPVIVYATLTIPQIMLAEAFLSFIGFGVQ
                     APLASWGSLAAEGIQNIGIFPWQLIFPGVTMALTLFSLNFLGDGLRDALDPQMRTV"
     gene            1486..2511
                     /locus_tag="VFO72_01825"
     CDS             1486..2511
                     /locus_tag="VFO72_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478053.1"
                     /translation="MTISSHSDGNLLEVKNLRTYFDSEDGVVKAVDGISFQLKRGETL
                     GIVGESGSGKSVTNLSIIRLVPDPPGKIVSGEVNFNGSDLLLLSNEAIRKIRGRRIAM
                     IFQDPMTSLNPFMKISKQLMEMTQLHLGHSKSQAYEHAIRMLETVGIPDARARADNYP
                     HEFSGGMRQRVMIAMALSCEPELLIADEPTTALDVTIQAQILELIKKLKSETGTSVIL
                     ITHDLGVVAGMTDHVVVMYAGKIFEQARTSELFSLPGNPYTKGLLKSVPDPTAEQGKL
                     YQIPGLPPDVAHLKPGCPFAPRCDRVEEICRREFPPFVQLNADHYSLFHFAREVYSES
                     GGTPIQS"
     gene            2508..>3103
                     /locus_tag="VFO72_01830"
     CDS             2508..>3103
                     /locus_tag="VFO72_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9478054.1"
                     /translation="MSATESILLSIRDLKVHFDLGGGGPLDKLFGTNPVKRVVKAVDG
                     VSIDLYKGETLGLVGESGCGKSTLGRAILRLTEPTAGQVFYQNRDLAHLSARQLREQR
                     RHLQIIFQDPYASLNPRMTIGQIVGEPLETFRLARGKEAEQRVQELLEVVGLSKRFIN
                     RYPHEFSGGQRQRIGIARALAVDPDFIVADEPISALDVS"
BASE COUNT          653 a          866 c          862 g          722 t
ORIGIN      
        1 ctactggttg ccaccttctc gatgggccgg ttttccgagt cgaaatctga ttctgccggt
       61 gctcactttg tcggcgttac agatggctta catcgcgcgg ctcacgcgcg ccgggatgct
      121 tgaagtgctg cgctccgatt atatccgcac ggcgcgcgcg aaggggttaa gcgagcgtga
      181 cgtagtgttg aagcacgcat tacgcggtgg cttgatgccc gtaatctcct acactggccc
      241 agcactggcg tttctattaa ccgggacggt ggtagtcgaa aaaattttcg cgttgcccgg
      301 tctgggaaac tatttcatcc aggcttcact caatcgcgat gagccgctga tcatcggcat
      361 cgtcgcgttt attgcaatca cgctgttgtt gatgaacctg ttggtcgata tcgcttatgc
      421 gtatctcgat ccacgcgtgc ggtaccagta aatacaaatg tctactgaga aggatccaga
      481 tcagatacac actcctccgg agatggcgat cgccgaggcc gacgtggagg cgggagagtt
      541 cgttcgcggc tcctcgttgt ggcgcgatgc gtggcgtcgt ttgcttaaaa acaagctggc
      601 ggtgttcggt ttgatagtgg ttgtgattgt gacgattgcg tcggtgcttg gtcctaccat
      661 aatcaagaac acctttggat tcacgcccga ctacattcct tcgcacgacg ttaaattaag
      721 cagatcctat cctcccttta ctggtccaaa cggcgaattt tcatggctcc acccgatggg
      781 gaccgacaac gccgggcgcg atcaactcgc tcgcgtgctt cagggtggac agatctcatt
      841 gtttgtcgga gtcatctcga cgctggtgtc gttgttgatt ggagtcactt acggcgcgat
      901 cgcgggctac gtgggcggcc gtctcgacaa cgtgatgatg cgcattgtcg acgtgttgta
      961 ctcgcttcca tacgtgatcg tcgtcatcgt gctgctctca atgtttcgga gtcagacgcc
     1021 gaggggtcag ttgatcctgc tgtttatcgc gctcggctcg atttcatggt tgacgatggc
     1081 caggatcgtg cgcggtcagg tgctatcgct caagaaccag gagtttgtgc tggccgcacg
     1141 agcgaccgga gtttcagcta cgcggatcat ctttcgtcac ctggtgccga atacgttggg
     1201 gccggtaatc gtgtacgcca cgttgacgat tccgcagatc atgttggctg aagcgtttct
     1261 ctcgttcatc ggttttggtg tacaggcgcc actggcgtca tggggaagtt tggcagcgga
     1321 gggcattcag aacatcggca tctttccgtg gcaactgatc tttcccggcg tcacgatggc
     1381 gctgacactc ttctcgctga attttctcgg cgacggcttg agggatgctt tggatccaca
     1441 aatgcgaacc gtataggtcc tatgacctat agggcctatt aaacgatgac cattagctcg
     1501 cacagtgacg gcaatcttct cgaagtaaaa aatctccgga cctacttcga cagcgaagac
     1561 ggagtcgtca aagccgtcga tggcatctcg tttcagctca aacgcggtga gacactcgga
     1621 atcgtaggcg agtcaggctc aggtaagtcc gttaccaatc tctcgatcat tcgcctggtc
     1681 cccgatcctc cgggcaagat cgtctccggc gaagtgaact tcaacggttc cgatctgctg
     1741 ctactttcaa acgaggcgat tcgcaaaatc aggggtcgcc gcatcgcgat gatcttccag
     1801 gacccgatga cctcactcaa tccgttcatg aagatctcca aacagctaat ggaaatgact
     1861 caactccacc tcggccactc taaatcgcag gcttatgagc acgcgatccg gatgttggaa
     1921 acagttggga tcccggacgc tcgcgcacgc gccgacaact acccgcacga gttttccggc
     1981 ggcatgcggc agcgcgtcat gatcgcgatg gcgctttcct gcgagccgga gctgctgatc
     2041 gctgacgagc caaccacagc gctcgacgtt acgattcagg cccagatact cgagctcatc
     2101 aaaaaactca agagcgaaac cggaaccagc gtcattctaa tcactcacga tctcggcgtt
     2161 gtcgcgggca tgacggatca cgtcgtcgtg atgtacgcag gcaagatctt cgagcaggcc
     2221 aggaccagcg aactgttttc gttaccggga aatccgtata ccaagggatt gctgaaatcc
     2281 gtgcccgatc cgacagcaga gcagggcaag ctgtaccaga ttccgggtct gccgcccgac
     2341 gtcgctcatc tcaaacccgg ctgtccgttt gcgccacgtt gcgaccgcgt ggaagaaatt
     2401 tgccggcgcg aatttccgcc gtttgtacaa ctcaacgccg accactattc actctttcat
     2461 ttcgcacgtg aggtctacag cgaatcggga ggcacaccga tccaatcatg agcgcaaccg
     2521 aatcaatatt gctttcgatc agggatctga aagtgcactt cgatctcgga ggtggtggtc
     2581 cactcgataa actctttggc actaatcccg tcaaacgcgt cgtgaaagcc gtggacggcg
     2641 tttccataga cttatacaaa ggcgagactc ttggactcgt gggcgaatca ggctgcggca
     2701 agtctacact cggacgcgcc atcctgcgct taaccgaacc gacagccggc caggtgttct
     2761 accaaaatcg cgatctggcg cacttgtccg cgaggcagtt gcgcgagcag cggcggcacc
     2821 ttcagatcat ctttcaggat ccttacgcgt cactgaatcc acgcatgacg atcggacaga
     2881 tcgtcggcga gccgctcgag acgtttcgcc tggcgcgagg gaaggaagct gagcaacgag
     2941 tgcaggagtt gctcgaagtg gtcggtctta gcaaacgttt catcaacaga tacccgcacg
     3001 agttttccgg cgggcaacga caacgcatcg gcatcgcgcg agcgctggca gtcgatcccg
     3061 atttcatcgt cgcagacgaa ccgatttcgg cgctcgatgt ttc
//
LOCUS       DASTEY010000108         5130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_216843, whole genome shotgun sequence.
ACCESSION   DASTEY010000108 DASTEY010000000
VERSION     DASTEY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216843"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..3834
                     /gene="smc"
                     /locus_tag="VFO72_01835"
     CDS             37..3834
                     /gene="smc"
                     /locus_tag="VFO72_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET9478055.1"
                     /translation="MFKLQRLEITGFKSFADYTEIIFTGDGITAIVGPNGCGKSNVAD
                     AIAWVLGEQRVKHLRGGEMKDVIFQGARNRPPSGMAEVVMHLVRDEVDEVEPDIEDID
                     STLEKIDDRLVNVEIEAADTADTETPTEPAATEPVTRITHKRHWRRRNLALEFAPGEA
                     VSVTRRLYRSGESEYLLNDRICRLRDIQDLFSGTGLAGGHYAIIEQGRIGQILSAKPM
                     DRRTIIEEAAGITKFRVRQRATEARLESARANLSRISDIVAEIDRQVNSLRRQAAKAR
                     RYGVLREELRELLRHVYLAEDRKLATVLEETHTKLVEVTDLELRISEDLSQKEEAARA
                     ATQQARAFEDELSSVRAAAAEAVLQRDRQARECVYQQEQLAEIEKRKGEVAAEIEALT
                     ARLVLIEAECHRLQHEDARLREESDRSALLLRTAEETYAERLAAATAAETEIENARSE
                     LLSQTAIAERLREIARQLETTLERLSQQAEGLAREGDRAASQHAERKLEAERFASELA
                     EARARVTRLQNEREFAVDAVVRGHEAVSDTEAELARVRDEYSRASHRLESLKELDQRR
                     AYYSPAVQLVFAPSETPRDFHFIGTLADALNVDAKWERAVEGVLGSSLQSIVVPTPAD
                     AVRGAQWLRENNGGRASFLVAGLHGGSDEVETITTLACRLEERPALSSGQVESFREEL
                     RISDLLGAPRELITVLKRTLPQKMNARLAANLEDAMIKSIATGEMYVTLNGDWIASGQ
                     FVNAGDARAIEEGAGLLAFKRELRELETRAETLAREVENATQLAKDARACLVELEDAV
                     VLLNEAIGREERDAMQRELTVSGLDQEVERAERHMRVVGQDSMRVDAERIEVEQRRAQ
                     ALLEAEAAEAAREAAADQVIKASASLTELRKAAELESESLAQQRAAAAAAAERRRATT
                     AELRRIEGELADFAARVDRYRMDLAEMDGRSGELKLSIADLELKASTVESDRAREEEQ
                     IATLSQKLQEARQQADEFSAELSELNRRAAEVRDARAALEVQRAEAQARQTFVHENCH
                     SELGQSLEDLAREITLGEDFDFDTSHARVEELRTRLDGFGAVNMMALEELAEAEERLL
                     FLTVQRQDIVEGIAATEEALREIKRRSRERFRHAFEQINKNFSVLFQELFGGGRGEMS
                     LIDADDVLESGIDVVAQPPGKRLQNVLLLSGGEKAMAALALVLGIFHYRPAPFCLLDE
                     VDAPLDEANVGRFTDKVIQMSANTQFIVITHNKRTMEMARALYGVTMEEAGVSKLVSV
                     KFE"
     gene            3849..4568
                     /locus_tag="VFO72_01840"
     CDS             3849..4568
                     /locus_tag="VFO72_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478056.1"
                     /translation="MRQGLPAALMILSAAISICGQDRSQTKHNPRPSQQAASPAVPAI
                     TGENSDPVKYTYEFSQPKFYIKHIVIEHDANGRGTVTFERLNEDGSVSEPLELSPAVL
                     SRLTASWQALQFLDSTADYQADKQFPHLGTMRIGMQRGDRKRVAEFNWTNNADANTLV
                     SEYRKAADQAILIFDISIARQNQPLNAPKLMEAMESLINRNALSDPKQLLPLLNELST
                     DERVPLIARNHALRLIKKIQK"
     gene            4565..5095
                     /locus_tag="VFO72_01845"
     CDS             4565..5095
                     /locus_tag="VFO72_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478057.1"
                     /translation="MTSALNRKEQLIVEVWAEMARESAGAAELEFIQQTLVERLGSQG
                     RESPAAIARTLADEGVRLQHPEILEADVRWRERHAVGLFTSEELNFTNIEVAIAWVEK
                     LATLPAPAELRAFVLQLKTELELVAKSMQIPLPDRLIALEVAHWLTVWLQNPPIFADW
                     LVLRRQSPEFRARFSS"
BASE COUNT         1165 a         1463 c         1546 g          956 t
ORIGIN      
        1 gtccccgagc tggcccaaaa cagagctttc tagctaatgt tcaaactgca acgcctggaa
       61 atcactgggt tcaaatcctt cgccgactac accgaaataa tcttcacagg agatggaatc
      121 accgccatcg ttggccccaa cggctgcggc aagagcaacg tggccgacgc gattgcctgg
      181 gttttgggcg agcagcgggt gaagcacttg cgcggcggcg agatgaaaga cgtcatcttc
      241 cagggcgcgc gcaatcgtcc gccgagtggc atggctgaag tcgtaatgca cctggtgcgc
      301 gacgaggtcg atgaagtcga gccggacatc gaggacatcg actccacgct cgagaaaatt
      361 gacgatcggc tggtcaacgt tgaaatcgag gctgccgaca ctgccgacac tgaaactcct
      421 acggagcccg ctgcgacaga gcccgttacg cggattacac ataagagaca ctggcgacga
      481 cggaacctgg ctctcgaatt cgcgcccggc gaagccgttt cagttacacg tcgactctat
      541 cgttccggcg aaagcgaata tctgctcaac gacagaatct gtcgccttcg ggacatccag
      601 gatctcttct ccggcaccgg tctcgccggt ggccactacg caatcatcga acagggtcgc
      661 attggccaga ttctttccgc gaagccgatg gaccgccgca cgatcattga agaggcggcc
      721 ggtatcacaa aatttcgcgt gcgacagcgc gcaaccgaag cccggcttga atcagcgcgc
      781 gcgaatctct cacgcatctc cgatatcgtc gccgagatcg atcgccaggt aaacagcctg
      841 cgccgccagg cagccaaagc ccgacgttac ggcgttttgc gcgaagagct gcgcgagctg
      901 ctgcgtcacg tttacctcgc cgaggaccga aagcttgcga ctgtactcga agagacgcac
      961 acgaaactcg tcgaagtcac tgatctcgaa ctgcgtatca gcgaggatct cagccaaaag
     1021 gaagaagccg cccgcgccgc cacacaacag gcccgagcat ttgaagacga gctatctagt
     1081 gtgcgtgccg ctgcggccga agcagtactg caacgcgatc gacaggcgcg cgagtgcgtt
     1141 tatcagcagg aacagttggc cgagatcgag aaacgcaaag gcgaagtggc cgcggaaatt
     1201 gaagcgctca ccgcgcggct tgtgttgatt gaggcagaat gccatcgatt gcaacacgaa
     1261 gatgcgcgcc tgcgcgagga gtctgaccgc agcgccttgc tgttgcgcac ggcggaggag
     1321 acttacgcgg aaagactggc cgctgccaca gccgccgaga ctgaaatcga gaacgcccgc
     1381 agcgaactgc tttcgcaaac tgccattgcc gaacgcttgc gcgagatcgc gcgccaactc
     1441 gaaacgacgc tcgaacgact gtctcaacaa gctgaaggtc tcgcgcgcga aggcgaccgc
     1501 gctgcaagcc aacacgctga aagaaaactc gaagccgaga ggttcgcgag tgaactcgcg
     1561 gaagcgcggg cccgcgtgac tcgtttgcag aacgaacgcg aatttgctgt cgatgcagtc
     1621 gtgcgtggtc atgaggcagt aagcgacacc gaagcggaac tggcgcgtgt gcgcgacgag
     1681 tactcgcgcg ccagtcatcg tctggaaagt ttgaaagaac tcgatcaaag acgcgcttat
     1741 tactcacccg cagtccaact tgtctttgca cccagcgaaa caccgcgcga ctttcatttc
     1801 atcggcactc tcgccgatgc tttgaatgtt gacgccaaat gggaacgcgc agtcgaagga
     1861 gtactcggtt cgtcgttgca gtcgattgtg gtaccgactc cggccgacgc cgtacgcggc
     1921 gcgcaatggc tgcgtgaaaa caacggcgga cgcgcgagtt tccttgttgc cggtttgcac
     1981 ggcggcagcg acgaagtgga gacgataacc acactcgctt gccggcttga agaacgtccg
     2041 gcgctgtcgt cgggccaggt cgagagtttc cgtgaggagc ttcgcatctc cgaccttctc
     2101 ggcgcgcccc gggaactcat caccgttttg aaacgaacgt tgccgcagaa gatgaatgcg
     2161 cgattggcgg cgaatctcga agatgccatg atcaaatcca ttgccaccgg cgagatgtac
     2221 gtcacgctga acggcgattg gatcgccagt gggcagttcg tcaatgcggg cgatgctcgc
     2281 gcgatcgaag aaggcgccgg actgctggcc ttcaaacgcg agttgagaga actggagaca
     2341 cgcgcagaga cgttggccag agaagtcgag aacgccactc aactggcgaa agatgcgcgc
     2401 gcgtgtctcg tcgaacttga agatgccgtt gttttgttga acgaggccat cggtcgcgaa
     2461 gagcgcgatg caatgcagcg tgagctgacg gtgagcgggt tggaccagga ggtcgaacgc
     2521 gctgagcgcc acatgcgcgt ggtgggccag gattcgatgc gcgtcgatgc cgaacggatc
     2581 gaagttgaac aacgtcgcgc gcaagcttta ttagaagcgg aagccgccga agccgcaaga
     2641 gaagcggcgg ctgaccaggt aatcaaagct tcagcatcgt tgaccgaact cagaaaagct
     2701 gccgagctcg agagcgaatc actcgcgcaa caacgtgccg cggcggccgc cgccgctgag
     2761 cgacggcgtg cgacgacggc ggaacttcgc cgcattgaag gtgaactggc ggacttcgcc
     2821 gcgcgtgtcg atcgctatcg tatggacctc gcggagatgg acggccgtag tggcgagttg
     2881 aaactctcca tcgcagatct cgaactcaag gccagcactg ttgaaagcga tcgcgcgcgt
     2941 gaagaagagc agatcgcgac gctctcgcag aagttgcagg aagcgcggca gcaagcggat
     3001 gaattctccg cagagttgtc ggaattgaat cgccgcgcgg ctgaagtacg cgatgctcgt
     3061 gccgcgttgg aagtgcaacg cgcggaggcg caggcgcgcc aaacgttcgt tcacgaaaac
     3121 tgccactccg agttgggcca gtcgctcgaa gatctggcac gtgagattac gctcggcgaa
     3181 gattttgatt tcgacacgag ccacgcgcgc gtcgaggagt tgcggacgcg gctcgacgga
     3241 tttggcgccg tcaacatgat ggcgctggaa gagctcgccg aagccgaaga acgtctgttg
     3301 ttcttgacgg tgcaacgtca ggacattgtc gaaggcattg ccgccaccga agaagccttg
     3361 cgcgagatca aacgccgttc gcgcgaacga ttccgccacg cgttcgaaca gatcaataaa
     3421 aacttcagcg tcttgttcca ggagttgttt ggcggcggac gcggcgagat gagcttgatc
     3481 gacgctgacg acgtgctcga gtcgggcatc gacgtcgttg cgcagccacc gggcaagcgt
     3541 ttgcagaacg tgctcttact ttccggcggc gagaaagcga tggcggcgct cgcgctggtg
     3601 ctgggcatct tccattaccg tccggcgccc ttctgtttgc tggacgaggt cgacgcgccg
     3661 cttgacgaag cgaacgtggg acgctttact gataaggtca tccagatgtc cgcgaacacg
     3721 cagttcatcg tgattacgca taacaagcgg accatggaga tggcgcgcgc tctttatggt
     3781 gtgactatgg aggaagcagg cgtctcgaaa ctggtttcgg taaagttcga gtgaatacaa
     3841 taaaatcaat gaggcaaggg ctccccgccg cgcttatgat tttgagcgcg gcgatttcca
     3901 tttgtgggca ggatcggtcg cagacgaaac acaatccgcg gcccagccag caggctgctt
     3961 ctccagccgt tcctgcaatc acgggcgaga attcggaccc ggtgaagtac acctacgagt
     4021 tttcgcaacc caagttttac atcaaacaca tcgtgatcga acacgatgcg aacgggcgtg
     4081 gcacggttac cttcgaacgt ctgaacgagg acgggtctgt gtccgagccg ctcgaactct
     4141 cgcccgcggt gctgtcgcgc cttactgcat cgtggcaggc gttgcagttt ctggattcaa
     4201 cggctgatta ccaggccgac aaacagtttc ctcatctcgg cacgatgcgc atcggcatgc
     4261 aacgcggcga tcgcaaacga gtggcggagt tcaactggac gaacaacgcc gatgctaata
     4321 cgctcgtcag cgaatatcgc aaggcggcag accaggcgat cttgatcttc gacatttcta
     4381 tcgctcgtca aaaccaacca ttgaacgcgc cgaaactgat ggaagcgatg gaatcgctta
     4441 tcaaccgcaa cgcactctcc gatccgaagc aactgctgcc gttgctcaac gaactgagca
     4501 ctgacgaacg ggtcccgttg atagcccgca atcacgcgct ccgcctcatc aaaaagattc
     4561 agaagtgacc tcagcgttga atcgcaagga acagttgatt gttgaagtgt gggcggagat
     4621 ggccagggag tcagccggcg ccgccgaact cgagtttatt caacagaccc ttgtagaacg
     4681 tctcggatct cagggccgcg aaagtcctgc tgcgattgcg cgcacgctgg ccgatgaagg
     4741 cgttcgactt cagcatccgg agattctcga agccgacgtg cgctggcgtg agcgccacgc
     4801 ggtgggcctg ttcacatccg aggagttgaa cttcacaaac attgaggtgg cgatagcgtg
     4861 ggtcgaaaaa ctcgctacgc ttcctgcccc agccgagcta cgagcctttg ttctgcaact
     4921 caaaactgaa cttgagttgg tcgcgaagag catgcaaatc ccgctgccgg accgcctgat
     4981 agctctggag gtcgctcact ggctaacggt ttggttacaa aacccgccca ttttcgcaga
     5041 ctggctggtt ctgcggcgcc aatcccctga atttcgcgcg cgcttcagtt cgtagacctc
     5101 ttatttctgg aactttccta cgcacggcgg
//
LOCUS       DASTEY010000109         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_958363, whole genome shotgun sequence.
ACCESSION   DASTEY010000109 DASTEY010000000
VERSION     DASTEY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958363"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1700
                     /locus_tag="VFO72_01850"
     CDS             111..1700
                     /locus_tag="VFO72_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478058.1"
                     /translation="MLSEKRQYSLHIWLLGLGFYLFYTPYSGLTKALSSGLLPGAGAV
                     KGTILLPLSAIGTVVGMMSFISVMGWWKYAGRRKFFGIEVPFPRRLTFLSGVCMATIM
                     GTTTLAFTFGGLSIVLVLVLLRAGTLIIAPIVDQIVGRRVRWFSWTAMLISLAAVLIA
                     LTDVRSYVLPIAAAIDIAAYLFAYFFKLQFMSRLAKSDDRAATLRYFVEEQMVASPLL
                     VLILIVLAVIGAGDVMMGFREGLTNFVTSQGAIYAALVGLFYAALCICTTMIFLDRRE
                     NTFCMPMHCGSSMLSGFTATAILAGLYNQSAPTMTQTVSAGFIAIALGFLSPLHHLDR
                     YLTKLRGARRERLFLFVCSGNTCRSPMAAAIANAEIAARLRANDFPQRRKGAKKPSES
                     VVLRVFASLREVLSVQALQTVNVRALSAGVAARVGEPLTAEAQEALRSLAVPVEPHAA
                     LNLTPELAQRAEMIFCMTSAHRAAVIQMLPAVASKTYCLDLEADIADPIGKGMEAYLN
                     CARRIHDLVRLRFDELELKAA"
     gene            1675..>2147
                     /locus_tag="VFO72_01855"
     CDS             1675..>2147
                     /locus_tag="VFO72_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478059.1"
                     /translation="MSSSLKLPKSDCARFARTEALDALGEASFAEFPAVRFPGTVLWC
                     NFELARELGFSVPRTNHLTPELHEELLNKLSLRALTPGDDVSDVEVVTLYADRYGGDG
                     VSPALGAGRAGFLRDGNLYIKGLGFTPLFRHNDKDDFVHSHGGVHLDDCMSEAIFG"
BASE COUNT          380 a          609 c          629 g          529 t
ORIGIN      
        1 tctcttttta tgttcgtcat gacaacgacg atcagattct cgcgacgatt ggtaagtcgg
       61 tattgggcct ggcccatgat ccgtctttct acaaaccgta gaacgccaac atgctgagcg
      121 aaaagcgcca atacagtttg catatctggc tcctggggct gggcttttat cttttctaca
      181 cgccttacag cggtctgacg aaagctctgt ccagtggtct gttgccgggc gccggcgcag
      241 tgaaagggac aattctgctg cctctctcgg cgattggaac ggtcgtcggg atgatgagtt
      301 tcatcagtgt tatgggctgg tggaagtacg ccgggcgtcg caagtttttt gggattgaag
      361 ttccctttcc gcgtcgcctg acgtttcttt cgggagtgtg catggcaacg atcatgggca
      421 cgacgacgct ggcgttcacg ttcggcggat tgtcgatcgt gctggtgctg gtgttgctgc
      481 gggccggcac gctgatcatt gctcccatcg tcgatcagat cgtgggccgg cgagtgcgtt
      541 ggttttcgtg gactgcgatg ctgatcagtc tcgctgcggt tctgatcgcg cttaccgatg
      601 tacggagtta cgtcttacct attgccgcgg cgatcgacat cgcggcctat ttgttcgcct
      661 acttcttcaa gttgcagttc atgagccggc tcgcaaagtc ggacgatcgc gctgcgacgt
      721 tgcgctactt tgtcgaggag cagatggtag cttcgccatt gctcgtactg atcctaatcg
      781 tgttggcggt gataggcgcg ggcgacgtga tgatgggttt tcgcgaaggt ttgactaact
      841 ttgttaccag tcagggcgca atctacgcag cgctggttgg tctcttttac gcggcactct
      901 gcatctgtac cacgatgatc tttctcgacc gtcgcgaaaa cactttctgc atgccgatgc
      961 attgcggctc gagcatgctc tcgggattta ctgccactgc catcctcgcc ggtttgtaca
     1021 atcagagtgc gccgacgatg acgcagacag tcagcgccgg ctttatcgcc atcgcgctcg
     1081 gatttctatc gccactgcat cacctcgacc gttacctcac gaaacttcgc ggcgcccggc
     1141 gcgagcgact attcctgttt gtttgcagcg gcaacacctg ccgttcgccg atggccgcgg
     1201 cgattgccaa tgcggagata gcggcgcgtt tgcgcgcgaa cgatttcccg caaaggcgca
     1261 aaggcgcaaa gaagccgagc gagtcggttg ttcttcgcgt ctttgcgtct ttgcgcgagg
     1321 ttctgtcggt tcaggcgttg cagaccgtca acgtgcgtgc tcttagtgcg ggagttgcgg
     1381 ccagagtcgg tgaaccgctt acggcggaag cgcaagaagc gctgcgttca ctcgcggtgc
     1441 cggtggagcc acatgcggcg cttaacctca cgccggagtt ggcccaacgc gcagagatga
     1501 tattttgtat gaccagtgcg caccgcgcgg ccgtgatcca aatgctgccg gcggttgcga
     1561 gcaagactta ctgtctcgac ctcgaggctg acatcgccga tcccatcggc aaaggaatgg
     1621 aagcttacct gaactgcgcg cgacgcattc acgatcttgt ccggttgcgt ttcgatgagc
     1681 tcgagcttaa agctgcctaa atccgattgc gcccgtttcg ccagaacgga ggcgctcgat
     1741 gcactcggtg aagcttcgtt tgctgagttt cccgcggtgc gctttccggg cacagtcctc
     1801 tggtgcaact tcgagctcgc gcgcgaactc ggattcagtg tgccgcgcac aaaccacctc
     1861 acgccggagc tgcacgaaga actgctcaac aagctttcgc tccgcgcact aactcccgga
     1921 gacgatgttt ccgacgtcga ggtcgttacg ctgtacgccg atcggtacgg cggcgacgga
     1981 gtcagtcctg ccctcggcgc gggccgcgca ggctttctcc gtgacggaaa tctttacatc
     2041 aaaggcctcg gcttcactcc ccttttccgt cacaacgata aagacgattt cgttcattcg
     2101 cacggtggtg ttcatctcga cgactgcatg tccgaagcta tctttgg
//
LOCUS       DASTEY010000110         3837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_431033, whole genome shotgun sequence.
ACCESSION   DASTEY010000110 DASTEY010000000
VERSION     DASTEY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431033"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFO72_01860"
     CDS             complement(<1..228)
                     /locus_tag="VFO72_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9478060.1"
                     /translation="MNCEQCQELIQDLIDGSISQRDEFTLNTHLKDCLDCESVRQDLT
                     SILTFCQMHRGEYEAPPNERALWLRIRNVIEA"
     gene            complement(397..978)
                     /locus_tag="VFO72_01865"
     CDS             complement(397..978)
                     /locus_tag="VFO72_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9478061.1"
                     /translation="MTAAETETPQTTTDYELAQRAAQGEMGAFEQLYQRHNRRVYSLC
                     LRMTGNVSEAEDLAQEVFIQLFRKIGSFRGESAFTTWLHRLTVNQVLMHFRKRGVRME
                     QTTEDGETPIQIVKGTENPNSMPVVDRIALDKAIAQLPPGYRTVFTLHDIEGHEHEEI
                     ARMLGCSVGTSKSQLHKARMKLRALLRQQKAAE"
     gene            complement(1243..2808)
                     /locus_tag="VFO72_01870"
     CDS             complement(1243..2808)
                     /locus_tag="VFO72_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET9478062.1"
                     /translation="MLFRTLSAAVYGIDADLVDIEVDLTQSRGESEQSSSVTIVGLPD
                     AAVRESRERIRAAMQNCGYFFPFQKTTINLAPADVRKEGASFDLPIALGILGANGDLA
                     EHYCLIHILCVGELSLDGRVRAIKGALPIAILAREASIRHVILPEESAKEAAVVDGVN
                     VYPVGDLRSAVRLIAELRSATPPMPLRVEAAEILSRAEQFTIDFKEVRGQVSAKRALE
                     IASAGGHNILLIGPPGSGKTMLAKRLPTIMPPLEFDAALELTKIHSVAGLTGRAGLVT
                     ERPFRSPHHTISDAGLIGGGSVPRPGEVSLAHQGVLFLDELPEFDRKVLEVLRQPLED
                     QKVTISRAAMSLTFPAAFMLAAAMNPCPCGYWSDPTRECRCSPLQIQRYIGRISGPLL
                     DRIDIHIDVPAVKFRELAGDVLPETDSSAAIRARVMTARERQIHRLRKERIFCNAAMT
                     PRMIRVHCKIDSTSERMLESAMMRLGLSARAYDRILKVSRTIADLEGCDKIGPTHVSE
                     AVGYRSLDRTYWA"
     gene            complement(2867..>3837)
                     /locus_tag="VFO72_01875"
     CDS             complement(2867..>3837)
                     /locus_tag="VFO72_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9478063.1"
                     /translation="PKAVSWAAASGLWQFIPSTGRMYGLRQNAYIDERNSFEQATRAS
                     ARYLKELAARYNGNWELAMAAYNTGEGNIDRAIARAGSANFWMIYPYIAQETRNYVPN
                     ILAVILIAKNPEKYGFKGIKPEPPMAYDVVRVPTATSLQLVADATDTSVDHIRALNPE
                     LKRDVTPRGDSYDVRIPPGRAKQFASLLQRISPDKRETARIISVAPGEDLQNVASRTG
                     ISVAQLQAMNGGVDLKSTTKLVVPSSSVKLTKWVRATAANNKAAAPSLNKVRARKGET
                     IASIAAARNLDANDVARLNGIPVDAELRAGQEVKLPATTAAPSRRR"
BASE COUNT          811 a         1097 c         1087 g          842 t
ORIGIN      
        1 ggcttcgata acgttgcgaa tgcgcagcca aagtgcgcgt tcatttgggg gagcttcgta
       61 ctcgccgcga tgcatctggc agaacgtcag aatcgacgtc aaatcctgcc ggacgctctc
      121 gcaatccaaa caatctttga ggtgcgtatt gagagtgaac tcatcgcgct ggctaatact
      181 gccatcgatt aggtcctgga tgagttcctg gcactgctcg cagttcattg cttaccacct
      241 tcgttctgtc gcaccaacgc gcagaattcg gaatgaaaac tgtttgtgtg acagaaccct
      301 agtccggttg tcatcaatca agggaagact gcgtaagctt ccgtgcttca ttaaattgaa
      361 tcgccaatga atcacttggg ctcatcacct tcgaggctat tcagcggctt tctgttgcct
      421 caaaagcgcg cgtaatttca tccgcgcttt gtgtaattgc gactttgagg tgccgaccga
      481 gcaccccagc atcctggcaa tctcttcgtg ttcgtgcccc tcaatgtcgt gaagggtaaa
      541 caccgtccgg taaccgggcg gcagttgggc gatagccttg tccaacgcaa tccggtcaac
      601 cacgggcatg gagttcggat tttccgtgcc ttttacgatc tgaatggggg tttcgccatc
      661 ctcggttgtc tgctccatgc gcacgccgcg tttgcggaag tgcatcagca cttgatttac
      721 ggtgagccgg tggagccagg tcgtaaaagc ggattctccg cgaaaactgc cgatttttct
      781 aaagagctgt atgaagacct cctgtgcgag atcttcagcc tccgagacgt ttccggtcat
      841 gcgcaagcag agcgagtaaa cccggcgatt atggcgctgg tatagctgtt cgaaagcacc
      901 catttcacct tgggcagccc tctgcgccag ctcatagtct gtcgtcgttt gcggtgtctc
      961 ggtttcggct gcggtcacgt gttcgcctgc tcggggacaa gcagagaaga atcttacaac
     1021 ttccgcagct tgttgggcac gatgctcaac gcgggctgtg atgcggcgct tcgcgcacgt
     1081 cgttgccttc ccgatccgta aaattactgg cttctcccag ccgaatcaat agtttcgcgc
     1141 cggttgggtg ggaaggatac gggcagacgt gtcgtggtga caagcgccgg cttgaagctc
     1201 tacgaggaca tcccgataat cctgaaatcc tgtcaaagag aactatgccc aatacgtgcg
     1261 gtccagggag cggtagccga cggcttcgga gacgtgcgtg ggaccgattt tgtcgcagcc
     1321 ctcgaggtcg gcaatcgtgc ggctgacttt gagaatacga tcgtaggcgc gggcggagag
     1381 accgagacgc atcatcgcgc tctccagcat tcgctcgctg gttgaatcga tcttgcagtg
     1441 cacgcggatc atgcgcgggg tcatcgcagc attgcagaag atgcgctctt tgcgcagacg
     1501 atgtatctgg cgctcgcgtg ctgtcattac gcgcgcgcgt atcgccgcag aactgtccgt
     1561 ttccggcaaa acatctcccg ccagttcacg aaatttcacg gccggaacgt cgatgtgaat
     1621 gtcaatgcga tccaacaatg gtccggaaat gcgaccgatg taacgttgga tctgcaacgg
     1681 tgaacaacgg cattcacgcg ttgggtctga ccaataaccg cacggacacg gattcatcgc
     1741 ggcggccagc atgaacgcag cgggaaacgt caacgacatc gcagcccgac tgatcgtcac
     1801 tttctgatct tcaagcggct gccgcaacac ctcgagaact ttccgatcaa actccggcaa
     1861 ctcgtccaga aagagcacgc cctggtgcgc cagtgaaact tcaccgggcc gcggcacgct
     1921 gccgccgcca atcaaaccgg catcactgat cgtgtggtgt ggcgagcgaa acggacgttc
     1981 cgtaaccaga cctgcacggc cggtcaagcc agcaaccgaa tggatcttcg tgagctccag
     2041 cgctgcgtca aattcgagcg gcggcatgat cgtcggcaga cgtttcgcca gcatcgtctt
     2101 gcccgatccc ggaggaccga tgagcagtat gttgtggcca ccggcactcg caatctccag
     2161 cgctcgcttc gcagagacct ggccacgcac ttccttgaaa tctatagtga actgttccgc
     2221 tcgcgaaaga atctccgccg cctcaactct caaaggcatg ggcggcgtcg ccgagcgcaa
     2281 ctcagcgatc agtctcacgg cgctgcgaag atctccaacc ggatagacgt tcactccatc
     2341 aaccaccgcc gcttctttcg cactctcttc cggcaagatg acgtgccgga tcgaagcttc
     2401 gcgcgccagg atggcaatcg gcaacgcgcc tttaatcgcg cgcacgcgac cgtcaagcga
     2461 aagctcaccg acgcagagaa tgtggatcag gcagtagtgt tcggcgaggt ctccgttcgc
     2521 gccgagaatt cccaacgcga tcggcagatc aaagctcgcg ccttctttgc gaacgtcagc
     2581 gggcgcgaga ttgatcgtgg tcttttggaa cgggaagaaa taaccgcagt tctgcatcgc
     2641 cgcgcgaatg cgttcgcgcg attcgcgcac agcggcatcc ggcaagccga caatcgtgac
     2701 ggaagacgac tgttcgcttt caccacgact ctgcgttaga tcaacttcga tatcgacgag
     2761 atcggcatcg atgccgtaga cggcggcgga aagagttctg aagagcatgt gaggaagctc
     2821 ctaatgttag agtgaggatc acagagaaca cggataatac agaaacttaa cgacggcgtg
     2881 agggtgcagc cgtggttgcc ggtagcttta cttcctgacc ggcgcgaagt tcggcgtcaa
     2941 ccggaattcc gttcaagcgc gcaacgtcat tagcatcgag gttgcgagca gcagcaatgc
     3001 tggcgatcgt ctcgcccttg cgagcacgaa ctttattcaa cgagggcgca gcagctttgt
     3061 tgtttgcggc cgttgcgcgc acccacttcg tgagtttcac gctgctgctc ggcaccacga
     3121 gtttggtggt cgacttcaga tcaactccac cgttcatcgc ctgcaattgc gcgacactga
     3181 taccggtgcg gctcgcaaca ttctgaagat cttcgccggg tgccacggaa attatgcgcg
     3241 ctgtctcgcg tttgtcaggc gagatgcgtt gcagcagcga ggcaaactgt ttggcgcgac
     3301 ctggcgggat gcgcacgtca taactatcgc cccgtggtgt aacgtcgcgc ttcaactcgg
     3361 gattcaacgc gcgaatgtga tcgacgctgg tgtcggtggc atcggcaacc agttgcagac
     3421 tcgtagccgt gggaacacga accacgtcgt acgccatcgg cggctcgggc ttgatgccct
     3481 tgaatccata cttttcagga ttcttcgcaa tcaggatcac cgcgagaatg tttggcacgt
     3541 agttacgtgt ttcctgcgcg atgtagggat agatcatcca gaaattcgcg ctgcccgcac
     3601 gtgcgatggc gcgatcgata ttgccttcgc ccgtgttgta agcggccatc gccagttccc
     3661 agttaccgtt ataacgggcg gcgagttcct tcagatatct agctgaagca cgcgtggctt
     3721 gttcaaaact attgcgctcg tcgatgtaag cgttctggcg caggccatac atgcgacccg
     3781 tgctcgggat gaactgccac aagccggaag ctgcggccca cgaaactgct ttaggct
//
LOCUS       DASTEY010000111         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_18796, whole genome shotgun sequence.
ACCESSION   DASTEY010000111 DASTEY010000000
VERSION     DASTEY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18796"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFO72_01880"
     CDS             complement(<1..410)
                     /locus_tag="VFO72_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9478064.1"
                     /translation="MKRIAVIPGDGIGPEVVREAVRVLKHLGTTRDIQLKFVDFDWGA
                     DKFLKEGVTLPDGALEMLSDDFDAILAGAFGDPRIPSNKHAEDILLGMRRGLDLYINL
                     RPVRLLDERLTPLANRKTSEINFVVFRENTEGAY"
     gene            complement(416..856)
                     /locus_tag="VFO72_01885"
     CDS             complement(416..856)
                     /locus_tag="VFO72_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9478065.1"
                     /translation="MLTSPEMLVPVDFSPCSVNALRVAIGMAAPEGDLTLLHVIDQQF
                     VDDAVAAGLGSSEDIRNRLKELAEANFSAMLEGIDAGQVDIEKMIVVGLPFIEILKIA
                     RDLDLPMIIMGVRGRSTPPEEVLFGSTAEKVLRGSRVPVLCVPY"
     gene            complement(1005..2018)
                     /locus_tag="VFO72_01890"
     CDS             complement(1005..2018)
                     /locus_tag="VFO72_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9478066.1"
                     /translation="MNSRKSTAQRAGLVLLALVSPLLLAACPKNGPPPRPQARISTGS
                     RPVERPKPIPTAFNGERAMDHARKQVEFGPRPPSSPALEKTRTYIVDQLKSYGLKVSL
                     DEFTAATPHGEKKMANIVGEISGSTKNIILLTSHYETKLYKNMQFVGANDPAASVGTL
                     LEIGRVLGSMNEKPKVTYRLVFFDGEESFCEGWSDCGSEDYPDNTYGSRHYVEQLRAR
                     NELENTRAMILLDLMGYKNLELGRDTLSTKWLQDIIWQTGRELGHEKIFVDRPEGIGG
                     DDHEPFLRAGVDAVDLIQLTGYPYWHQADDTLDKISARSMKIVGDTVLASLPKIVDKI
                     FDF"
     gene            complement(2023..2547)
                     /locus_tag="VFO72_01895"
     CDS             complement(2023..2547)
                     /locus_tag="VFO72_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9478067.1"
                     /translation="MSVSITFEPSGINGLVAEGTYLIDAARRMGAALGAGCTAGKGEC
                     PSCVVSVKTGAELLSSPSAAEEKQLGVEHLDQSYRLACQTKIENHGEVVVMVSTVTPR
                     RPTNVDVEAELRKKFGALPLSKKLATLMQLEAITMSEALDSAMEKPLQFGSKTFDALM
                     SRARAKNATVKPKK"
     gene            complement(2591..>3116)
                     /locus_tag="VFO72_01900"
     CDS             complement(2591..>3116)
                     /locus_tag="VFO72_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET9478068.1"
                     /translation="LRMLKSTASMTTEEPLTEEQEVFLKHIQEKGLKRTSQRDLILDV
                     FLRTEDHLSNEDLYQLVKKEDPTVGQTTVYRTLKILTDAGLAREVRFGDGRTHYEHNY
                     KHQHHDHMICSQCGKIIEFFSAELEALQDEMAAKHDFEITQHLLRIIGYCSDCRQTQE
                     QNTASSPANSTISP"
BASE COUNT          635 a          829 c          864 g          788 t
ORIGIN      
        1 cagtaagcgc cttcagtgtt ttcacgaaag acgacaaaat tgatctctga agttttccga
       61 tttgccagag gcgtgagccg ttcgtcgagt agccgcacgg gccgcaggtt gatgtataga
      121 tcgagaccgc ggcgcatacc gagcaggatg tcttcggcat gtttgttgga aggaattcgc
      181 ggatcgccaa acgcgcccgc gagaatcgcg tcgaagtcgt ccgacaacat ctcgagcgcg
      241 ccgtccggca gcgtgacacc ttcttttaga aacttgtcag cgccccagtc gaaatcgaca
      301 aacttgagct ggatatctct cgtcgtgccc agatgtttca gcacgcgcac cgcttcgcga
      361 actacttccg gaccgattcc gtctccagga ataactgcga ttcgtttcat cagattcaat
      421 aaggcacgca gagcacgggc actcgcgagc cgcgcaaaac tttctcggca gttgagccaa
      481 acaacacttc ttccggcggc gtcgaacgtc ctctgacgcc catgatgatc ataggcagat
      541 cgaggtcgcg cgcgatcttg aggatctcga tgaaaggcag gcccaccacg atcatctttt
      601 cgatgtcgac ttgtcctgcg tctattcctt ccagcatcgc actgaagttt gcttccgcca
      661 gttctttcag acgatttctg atgtcctcac tcgatcccaa accagcagca acggcatcat
      721 cgacaaactg ctggtcgatg acgtgaagca acgtcagatc gccttccggc gcggccattc
      781 cgatcgccac tctcaatgcg tttaccgaac acggcgaaaa atctaccggg acgagcattt
      841 caggtgatgt aagcatcggc ctcccatttt tgatttttga tttttgattg tcgattttta
      901 attttccatt gttgattgtt gattgttgat ggggttggtt taacgcaatc ggcaattggc
      961 aatcggcaat cggcaatcag caatcagcaa tcaaaaatca aaaatcaaaa atcaaaaatt
     1021 ttatcaacta tcttcggcaa actcgcgagc acggtatcgc ccacgatctt catactgcgc
     1081 gccgagatct tatctagcgt gtcgtcagcc tgatgccaat aaggataacc cgtgagttga
     1141 attagatcga cggcatcgac tccggcgcgc agaaacggtt cgtgatcgtc gccaccaata
     1201 ccttcgggtc gatccacaaa gatcttttca tgtcccagct cgcggccagt ttgccaaatg
     1261 atgtcctgca accacttcgt gctcagtgtg tcccggccga gttcgaggtt cttgtaaccc
     1321 atcagatcga gcaggatcat cgcgcgcgtg ttttccagtt cgtttctcgc tcgcaactgc
     1381 tcgacgtaat gacggctgcc gtaagtgtta tctggataat cttcgctgcc gcaatcactc
     1441 cagccttcgc agaacgactc ttcgccatcg aagaacacga gccggtaggt gaccttcggc
     1501 ttttcgttca tgctgccgag cacgcgcccg atctcgagca gcgtgccgac agaagccgcg
     1561 ggatcgttcg cgccgacgaa ctgcatgttt ttgtagagct tcgtctcgta gtgactggtg
     1621 agcagaataa tgtttttcgt tgagccggaa atctcgccaa cgatgttcgc cattttcttc
     1681 tcgccgtgcg gcgttgcggc tgtgaactca tcgagcgaga ccttgagccc gtaagacttc
     1741 agctggtcaa cgatgtaagt gcgcgtcttt tccagcgcgg ggctggaggg aggtcgtggt
     1801 ccaaactcga cctgcttgcg tgcgtggtcc attgcacgtt cgccgttgaa ggccgtggga
     1861 atcggtttgg gtcgttcgac cggacgagat ccggtcgaga tgcgcgcttg aggtcgtggc
     1921 ggcggaccgt tcttcggaca agcagcaagg agcagcgggc tgactagcgc cagcaggaca
     1981 agaccggcgc gttgagctgt gctcttgcgg ctgttcatcg catcatttct tcggcttgac
     2041 ggtcgcgttt tttgcgcgtg cgcgactcat cagcgcgtcg aaagttttcg agccgaactg
     2101 taatggcttt tccatcgcgg agtcgagcgc ttcagacatc gtgatcgctt cgagttgcat
     2161 gagggtcgcg agcttcttac tcaacggcaa ggcgccgaac ttcttgcgca actcggcttc
     2221 gacgtcgacg tttgtgggac gtcttggcgt aacggtgctg accatcacca caacctcgcc
     2281 atggttttcg atcttggtct gacaggcaag acgatacgac tgatcgaggt gttcgacgcc
     2341 gagttgtttc tcttcagcgg cgctgggcga tgaaagtagc tcggccccgg tcttcacgga
     2401 aacgacgcac gaggggcact cgccttttcc cgcggtgcaa cctgcgccga gcgcagcgcc
     2461 catccgtcgc gcggcatcga tcaggtaagt tccttcggca acgagtccgt taattccgga
     2521 aggttcaaat gtaatcgaaa cgctcatttc aacctccatt aattctggtt cgtgtgatgt
     2581 ttcaaagcgt tcaggggctg atggttgagt tggccgggga gcttgctgtg ttctgttctt
     2641 gcgtctgacg gcagtcggag cagtagccga tgatgcgcag caggtgctgg gttatttcga
     2701 agtcgtgttt tgcggccatt tcgtcctgca aagcttccag ttcggcggag aaaaactcga
     2761 tgatcttgcc gcactgcgaa cagatcatgt gatcgtgatg ttgatgtttg tagttgtgtt
     2821 cgtagtgggt acgtccatcg ccgaagcgca cttcgcgcgc gaggccggcg tcggtgagaa
     2881 ttttgagggt gcgatagacg gtagtttggc cgacagtggg atcctctttt ttgacgagct
     2941 ggtacaggtc ttcattcgag aggtggtcct cagtgcgcag aaagacatcc aggataaggt
     3001 cgcgctggga ggtgcgtttc aggcctttct cttggatgtg tttgagaaag acttcctgct
     3061 cttcggtcaa tggttcttct gtcgtcatac ttgccgtgga ttttagcatt cggagg
//
LOCUS       DASTEY010000112         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1568115, whole genome shotgun sequence.
ACCESSION   DASTEY010000112 DASTEY010000000
VERSION     DASTEY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568115"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..679)
                     /gene="moaC"
                     /locus_tag="VFO72_01905"
     CDS             complement(212..679)
                     /gene="moaC"
                     /locus_tag="VFO72_01905"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET9478069.1"
                     /translation="MTQFSHVDDEGRVRMVDTSDKETSSRRAVASARVLMRPETVTAL
                     REHRTPKGDPLEAARLAGIMAAKRTAELIPLCHPLPLTHIDVRAELDETGVSLEAEVA
                     TRAQTGVEMEALTAVSVAALTVYDMCKALEKGMTITDVRLQRKTGGKSGDYSA"
     gene            914..1759
                     /locus_tag="VFO72_01910"
     CDS             914..1759
                     /locus_tag="VFO72_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET9478070.1"
                     /translation="MFETDKEVLDWYERQPRALSREYVNNIRWSEIKNYPINPAFVPV
                     LIYMRDIEYFTDMYYQELRRTPTGKDPIIRKFMDRWSIEELHHANLLNRFLEEAGYPS
                     GKDWRFEATRKIPKLYTIGSYLIDHASRPFGKYFHGAHMVWGAINEITAMQAYRRLSD
                     LAGHPVLKELLLGIVQEESIHGSFYWNVARVKLAEAKFSRDLARFVIGKFWSPVGQGA
                     KRRAEANYVMATLFPGKEGLELFDQRIGNRIERLPGFVGFKGLTERIAPIVQVKSEVT
                     ANATP"
     gene            complement(1737..>2147)
                     /locus_tag="VFO72_01915"
     CDS             complement(1737..>2147)
                     /locus_tag="VFO72_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET9478071.1"
                     /translation="EIFFERVALRPGKPTVFARLPNGTLVFGLPGNPVSVAVTFNLFA
                     RTALLAMQGVTEPALKHETAMLARGVKGNVERESFLPAQLTTNDDGELIAFPLKWGGS
                     SDFVAFALTTALVIVPAGTRAIEANSLVRVLRLP"
BASE COUNT          501 a          602 c          584 g          460 t
ORIGIN      
        1 cgaaacggat acgtcaggac ttcggcgtaa ggaatgaacc agagcaacac tgaaaccgtc
       61 gctgcgacga ggagtgtcat ggcctgcggt cgcgcgtctt gtgaaagctt caatttcatc
      121 ttaaaaactc cggctggctg atttctctgc tattttagtt cgcacgggct aaaacgcaca
      181 acatcaacgg tatgttcccc aaagtatgcc gtcaggctga gtagtccccg gattttccgc
      241 ccgttttgcg ctgcagacga acgtccgtaa tagtcatccc tttttccagc gccttgcaca
      301 tgtcgtaaac ggtcaacgcc gcaaccgaaa cagccgtcaa cgcctccatt tccacgcccg
      361 tttgcgccct cgtcgcaacc tccgcttcca gcgacacacc cgtctcgtcc aactcggccc
      421 gcacgtcgat atgcgtcaaa ggcagcggat gacaaagcgg aatcaactcc gcagtgcgtt
      481 tggctgccat gatccccgcc agccgtgcgg cctcgagtgg atcgcctttc ggcgttcgat
      541 gttcgcgcaa cgcagtcaca gtctcaggcc tcatcagaac gcgcgcggat gcgacagcac
      601 gacgcgaact tgtttccttg tcgctcgtat caaccatgcg aacgcggcct tcgtcgtcga
      661 cgtgagagaa ctgtgtcatg gcgaaaagta gataacaaaa actcggcccg aagcaagtag
      721 atctcgacat cagacggcgg ttaggtctat tctctgccgc gaccgggcgc gcgccgctta
      781 cgcggccggt ttatgctcca cctattctcc gcagtccagt ttttccagat aagtgtgggc
      841 ggcagcggaa tccattttgg gtagttagac gtacatttag agaccaactg aaaagcgcct
      901 ctggaggtta tctgtgttcg aaactgacaa agaagtgctt gactggtatg agcgccaacc
      961 gcgcgcccta tcaagagagt acgtgaacaa catccgctgg agcgagatca agaactaccc
     1021 gatcaatcct gcgtttgtgc cggtgctgat ttacatgcgc gacatcgagt acttcactga
     1081 catgtactat caggaactac gacgcacgcc gaccggcaaa gatccgatca ttcgaaagtt
     1141 catggaccgc tggagcattg aagagttgca tcatgccaac ctgctcaatc gttttctcga
     1201 agaagctggt tacccgagcg gcaaagactg gcgctttgaa gcaacacgga agatcccaaa
     1261 gctgtatacc atcggaagtt atctaatcga ccacgccagc cggccgtttg gaaaatactt
     1321 tcacggcgcc cacatggtct ggggcgcgat caacgagatc acagccatgc aggcgtatcg
     1381 ccggctttca gatctcgccg gccatccggt gctgaaagag ttgttgctgg ggatcgtgca
     1441 ggaagagtcg attcatggaa gtttttactg gaacgtcgca cgcgtgaaac tcgcagaggc
     1501 gaagttctcg cgcgatctgg ctcgattcgt catcggaaaa ttctggagcc ctgtgggcca
     1561 gggtgcgaag cgacgagcgg aagcgaatta cgtgatggca acactctttc cgggtaaaga
     1621 aggtctggag ctgttcgatc agaggatagg aaatcgcatc gaacgtctgc ccgggtttgt
     1681 cggattcaag ggattaactg aacggattgc gccgatagtg caggtaaagt ctgaggttac
     1741 ggcaaacgca acaccctgac gagcgaattg gcctcgatcg ctctcgttcc ggcgggcacg
     1801 atcacgagcg cggtcgtgag tgcgaaggct acaaaatctg acgagccgcc ccacttcagc
     1861 ggaaaggcga tcaattcacc gtcgtcgttg gtggtgagtt gcgccggtag aaaactttcg
     1921 cgttcgacat ttcctttcac accacgagcc agcattgccg tttcgtgttt cagcgccggc
     1981 tccgtcactc cctgcattgc cagcagcgcc gtccgcgcga aaagattaaa cgtcactgcg
     2041 actgatacgg gattacccgg caggccaaac acgagcgttc catttggcaa acgtgcgaag
     2101 acggttggtt tgccgggacg cagcgcaact cgttcgaaaa agatctc
//
LOCUS       DASTEY010000113         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1584554, whole genome shotgun sequence.
ACCESSION   DASTEY010000113 DASTEY010000000
VERSION     DASTEY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584554"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..528
                     /locus_tag="VFO72_01920"
     CDS             133..528
                     /locus_tag="VFO72_01920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9478072.1"
                     /translation="MSYRRDACGPTIQIPDKEHLVADDNDDEFESQEERRAYDRSRLI
                     VDVFFDGQDVTGVASTKDISPGGLYMNTQAPIPEGAMLLVRIPFRPDAEVVCRAEVVY
                     SEPGRGVGVRFCDLTEEGRALLDREVSHG"
     gene            521..1045
                     /locus_tag="VFO72_01925"
     CDS             521..1045
                     /locus_tag="VFO72_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478073.1"
                     /translation="MGDIEPVLTWTTLSAETREALAGKLAGLWGSPTDAAAFDSWPLD
                     KKQALLLLFHRMYEKGLWNLVRRVTNVYGEGGVGLEFEAWPKIESTLSRRRDFTRRLA
                     NHGDTSGGFYEKNRDTVILHFLFQEGEPRRWYVHFDLYSPVHSPRSVFRHLRHEYLGK
                     TKPDWRLIAKHLNP"
     gene            1063..>2100
                     /locus_tag="VFO72_01930"
     CDS             1063..>2100
                     /locus_tag="VFO72_01930"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HET9478074.1"
                     /translation="MFDQFTLGIEEEFQIVDPHTRELRSHVAEILEEGKMLLGEQIKP
                     EMIQSMVEVGTGICRNIQEAREDITRLRSIIAGLAKKNGLVIVAASTHPISRWQDQKI
                     FDDERYELLIQELQTVARSLLIFGLHVHVGIPDPERAVHIMNAARYFLPHVLALSTSS
                     PFWMGHNTGLKSYRTEIFKQFPRTDIPDHFDSYSSFQRYVNLLVKTGCINDGKKIWWD
                     IRPHPYFPTLEFRICDIPTRVDDTIAIAALFQAIVAKLNSLIDRNLGFRLYRRMLIQE
                     NKWRAVRYGLDGKMIDFGKEKEVPVRDLVLELLEFVDDVVDGLGSRKEIEHIHTILER
                     GTSADRQLEVYR"
BASE COUNT          460 a          577 c          622 g          441 t
ORIGIN      
        1 aactggccaa cgtaagttcc ggcggcggca tggtgcccac ctacatcatc agcgaaaggt
       61 aacgaaaaag ggtacaattc gagccctggg accgcaggcg tctcgcctgc accgtacgag
      121 tcagacgctg ccgtgagtta cagacgagac gcctgcggtc ccacgattca aattcccgac
      181 aaggagcatc tcgtggccga cgataacgac gacgagttcg aatcgcagga ggagcggagg
      241 gcttacgacc gttcccgttt gatcgtagac gtgtttttcg atggccagga cgtcacaggc
      301 gtggcgagca cgaaagacat cagtcccggc ggactctaca tgaacacgca ggcgccgatt
      361 cccgaaggtg cgatgctttt ggtgcgaata ccttttcgtc ctgacgcgga ggtagtatgc
      421 cgcgcggaag ttgtttacag cgaacccggt cgcggcgtcg gcgtccgttt ttgcgacctt
      481 accgaggaag gacgcgctct gttggatcga gaagtttcgc atgggtgaca ttgagccggt
      541 tttgacatgg accacactgt ctgccgagac gcgtgaggcg ttggcaggaa agttggccgg
      601 cttgtggggc tcgcccacgg acgcggcggc gtttgactcg tggccgctcg ataagaagca
      661 ggcactgctg ttgctgttcc atcggatgta cgagaaaggc ttgtggaacc tggtgaggcg
      721 ggtcaccaac gtctacggcg aaggtggcgt tgggttggag tttgaagcgt ggccgaagat
      781 tgagtcgacg ttgtcgcggc gtcgagactt tacgaggcgt ctcgctaatc acggcgatac
      841 gagcggcggt ttctacgaaa agaatcgcga caccgtaatc ctgcactttc tctttcagga
      901 aggcgaacca cgccgctggt acgtgcattt tgatctttac agtccagttc actctcctcg
      961 cagcgtcttc agacacctgc gccacgagta cctgggcaag acgaaacccg actggcgact
     1021 gatcgccaaa cacttgaatc cctaatgtca aaaggggccg ccatgttcga tcaatttacg
     1081 ctgggcatcg aagaggaatt tcaaatcgta gatccgcaca cgcgcgaatt gcgttcgcac
     1141 gtcgccgaga tcctcgaaga aggcaagatg ctgctgggcg agcagatcaa gcccgagatg
     1201 atccaatcga tggtcgaggt gggcaccggc atctgccgca acatccagga agcgcgcgaa
     1261 gacatcacgc gcttgcgcag tatcattgcg ggactcgcga aaaagaacgg gctggtgatc
     1321 gttgccgctt ccacgcaccc gatttcacgc tggcaggacc agaagatctt cgacgacgaa
     1381 cgatacgaac tccttattca ggagttacag actgtggccc ggtcgctttt gatcttcgga
     1441 cttcacgttc acgtcggtat tcccgatccg gagcgcgcgg tccacataat gaacgcggcg
     1501 cgttatttcc tgccgcacgt gcttgcgctc tcaacgtcgt cgccgttctg gatgggccac
     1561 aacacgggcc tcaagtcgta tcgaacggaa atcttcaaac agtttccccg caccgacatc
     1621 cccgatcact tcgattccta cagcagcttt caacgttacg tgaatctgct ggtgaagacc
     1681 ggttgcatca acgacggaaa gaaaatctgg tgggacatcc ggccgcatcc atactttccg
     1741 acgctcgagt ttcgcatctg cgacattccg acgcgcgtgg acgacacgat tgcgattgcc
     1801 gcgctgtttc aggcgatcgt tgccaagctc aacagcctga tagaccgaaa cctcggcttc
     1861 cggctttatc gccgtatgtt gattcaggaa aacaagtggc gcgctgtgcg ttacggcctg
     1921 gacggcaaga tgatcgactt cggcaaggag aaggaagtgc cggtgcgcga tcttgtgctg
     1981 gagttactgg agtttgttga cgacgtagtt gacggtctcg gctccagaaa ggagatcgag
     2041 cacattcaca cgattctcga gcgcggcacg tcagccgatc ggcagcttga ggtctatcgg
//
LOCUS       DASTEY010000114         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_563039, whole genome shotgun sequence.
ACCESSION   DASTEY010000114 DASTEY010000000
VERSION     DASTEY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563039"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFO72_01935"
     CDS             <1..795
                     /locus_tag="VFO72_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478075.1"
                     /translation="VEVTQQLTLLTAITDGPVSLDWSQAVALDLPVEDLEPEPQPESQ
                     FAEVPANATKVKSYATWRKDLASWIYRNQRLELLESPSLDIVSNPGESERDFRVRLQQ
                     LAREQRDEAVEKLRRKYAPKFEQLEERKRRAEQAVAREMEQAKGQKMQTAISFGATLL
                     GSFLGRKSLGRATTAVRGVGRSVNEAQDVERAEENVAALSQKLSDLDAEFQAETAALE
                     RSFDPQTEQLEKVSLKPTKANIAVKLVTLAWSPYWHESGEVKPAWE"
     gene            828..1277
                     /locus_tag="VFO72_01940"
     CDS             828..1277
                     /locus_tag="VFO72_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478076.1"
                     /translation="MKIPLKYGLLITLGVMVWVLIARNAVTNPLSPVHTLGTPIFINL
                     LQFVMIYLGLKALEHEKGESPTFKEGLKTGVAISFVYAITAVLFFIGVVMVVGTRWLA
                     SEPGAAEAPMSRVLAGAFIGLFLGAMLFGLIYSTVISFFIAKRRSEE"
     gene            complement(1305..2321)
                     /locus_tag="VFO72_01945"
     CDS             complement(1305..2321)
                     /locus_tag="VFO72_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HET9478077.1"
                     /translation="MRQIVTTGTGDIDVLKIEERPDPTPGEGEVLIRVRAAGLNFADI
                     LSRQGLYPDSPPKPCVMGYEVSGVVEAAGKDVNSSFVGKSAVALTRFNGQSELIAVKA
                     TQMFEKPETLTFEQGAAIPVNYLTAYALLVVMGSLHAGESVLIHNAGGGVGLAALDIA
                     KKIGAETYGTASPSKHKFLTERGLDHPIDYRTQDWLPELQQLTNGRGVDLVVDPIGGA
                     HWKKSYAALRSTGRLGMFGVSAASANGFSGKLKMLKAAIQTPWFHPFPLLNKNRGVFG
                     LNLGHMWHEPEKVAVWMRDILRGVEEEWVRPHVDQAFVFADVGKAHRYLEERKNIGKV
                     VLVP"
BASE COUNT          560 a          732 c          685 g          534 t
ORIGIN      
        1 gttgaagtga ctcagcagtt gacgctccta accgcgatca cggatggacc agtcagcctt
       61 gactggagtc aggctgtcgc attggatctt ccggtcgagg atctcgaacc ggagcctcaa
      121 ccagaatctc agttcgcgga agtgccggcc aacgcgacta aagtgaagag ctacgcgacg
      181 tggcgcaaag atctagcgag ctggatctat cgtaatcaac gtctcgaact actcgagagt
      241 cccagtctgg atatcgtctc aaatccgggc gagagcgaac gtgatttccg agttcgcttg
      301 cagcaactcg cgcgcgagca gcgtgatgaa gcggttgaga agctgcgacg gaagtacgcg
      361 ccgaagttcg aacaactcga ggagcggaag cgccgtgcgg agcaagccgt cgcgcgcgag
      421 atggagcagg caaagggtca aaagatgcag accgcgatct cgtttggcgc gacgctgctc
      481 ggatcgtttc tcgggcgaaa atcgctcggc agagcaacta cggcggtgcg cggtgtgggt
      541 cgttcggtga acgaggcgca ggatgtggag cgcgcggaag agaatgtcgc cgcattgtcg
      601 caaaagctca gcgatctcga cgctgagttt caagccgaaa ccgcagcgct ggaacgatct
      661 ttcgatccgc agacagaaca gctcgagaaa gtcagtttga agccaactaa agcaaacatc
      721 gcagtgaaat tagtaacact tgcgtggtcg ccttactggc atgagtccgg cgaggtgaaa
      781 cctgcgtggg aataatttag gccgcagatt ttcgcggata aaggcggatg aaaataccac
      841 taaagtatgg attattgatc acgcttggcg tgatggtgtg ggtgcttatc gcgcgtaacg
      901 ccgtcacgaa tcccttgtcg cccgttcaca cgctcggcac gccaatcttc atcaacctgc
      961 ttcagttcgt catgatctat ctcggactga aggcgttgga gcatgagaaa ggcgaaagcc
     1021 caacgttcaa agaaggattg aagaccggcg tggcgatctc atttgtctac gcgatcaccg
     1081 cagtgttgtt ctttattggc gtggtcatgg tagtgggcac gaggtggctg gcgagtgagc
     1141 cgggagcggc cgaagcgccg atgtcgcgag ttctggcagg cgcgttcatc ggcttgtttc
     1201 tcggtgcgat gttgttcggc ctgatctact cgacggtgat ctccttcttc atcgcgaagc
     1261 gccgatcgga agaataggtc caataggacc tataggacct attcttaggg caccagcacg
     1321 accttgccga tattctttcg ctcttccaaa tagcgatgcg ccttcccgac atcggcaaac
     1381 acaaatgcct gatcgacgtg cgggcgcacc cattcctctt ccacgccgcg cagaatgtcg
     1441 cgcatccaca ccgccacctt ctccggctcg tgccacatgt ggcccaggtt caacccaaac
     1501 acaccacgat tcttgttgag tagcggaaac ggatgaaacc acggcgtttg aatcgccgcc
     1561 ttcaacattt tgagcttgcc tgagaacccg ttcgccgatg ccgccgagac tccaaacatc
     1621 cccaaacgtc ccgtcgatct aagcgccgcg taactcttct tccaatgtgc cccgccaatc
     1681 ggatcgacaa cgagatcgac gccacgtccg ttagtgagct gctgcaattc cggcagccag
     1741 tcctgcgtgc ggtaatcgat cgggtggtcc aaacctcgtt ccgttagaaa cttgtgcttg
     1801 ctcggactcg cagtgccata agtctcggcg ccgatcttct tcgcgatgtc gagcgccgcg
     1861 agcccaacac caccgccggc attgtggatc aaaaccgatt cacccgcgtg cagcgaaccc
     1921 atcaccacca gcagcgcgta agcggtcagg tagttcaccg gaatcgccgc cccctgttca
     1981 aacgtcaacg tctccggctt ctcgaacatc tgcgtcgcct ttactgcgat taactccgat
     2041 tggccgttaa agcgagtcaa cgcaaccgca gactttccaa caaacgagct gttcacatct
     2101 ttgcccgccg cttcgaccac accactcacc tcgtagccca tcacgcaagg cttcggcggc
     2161 gaatccggat acaaaccctg gcgcgacagg atgtcggcaa agttcaaacc ggcagcccga
     2221 acacggatca gcacctcgcc ttcaccgggt gtgggatcgg gccgttcctc gatcttcagc
     2281 acgtcgatat cacccgtgcc ggtcgtcact atttgtcgca tagaccaagt ataagttagc
     2341 tcagggcttt tgcaggtacg tgtttggctt tggcgttggt gcgaaccgat cgcctgccgc
     2401 ctcctcaaaa caaatcttgt cagccctcac ctgatgccat tgccagatat tcgattgcct
     2461 gctctctcaa gacctgttta tcgcgaggct ctcgataatc agctttggca t
//
LOCUS       DASTEY010000115         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1617694, whole genome shotgun sequence.
ACCESSION   DASTEY010000115 DASTEY010000000
VERSION     DASTEY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617694"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFO72_01950"
     CDS             complement(<1..180)
                     /locus_tag="VFO72_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478078.1"
                     /translation="MSTKVEATIDDLYKVEGKAELVNGEIVLMPLTGALPGYAGDEIF
                     SSLRDYSRRMKFGRAF"
     gene            complement(192..1214)
                     /gene="moaA"
                     /locus_tag="VFO72_01955"
     CDS             complement(192..1214)
                     /gene="moaA"
                     /locus_tag="VFO72_01955"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET9478079.1"
                     /translation="MKDSLQNTVLRDSYGRAIRDLRVSLTDRCNFRCFYCLPHGEPPI
                     APKEQMLSFEEIDYVCEIFVSLGIEKIRLTGGEPMLRRDIETIIRKLSRLKTAEPRLV
                     DLALTTNGYFLPERAHALKDAGLDRVTISLDSLKRDVFKRMTGVDVLDKVLAGIAAAK
                     DAGLDPIKINAVIVRGHNEDEVADFAAFAREYDVKMRFIEFMPLDSGHEWSRADVVSG
                     QQIKDRINERYPLIKLAIKRGSETSARYRFADGAPGEIGIIAPVTEPFCGACSRIRLT
                     ADGQIRTCLFSTVEHSLRDVVRSGASRAEIVDYIESVILKKEPRHFINDPEFVAPSRT
                     MSFIGG"
     gene            complement(1262..2437)
                     /locus_tag="VFO72_01960"
     CDS             complement(1262..2437)
                     /locus_tag="VFO72_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478080.1"
                     /translation="MCLIHSIGGAFNHAPQELTEKITQRASDLIKRAFDDIDPKRLVD
                     HHVHIAGLGSNGSRAFVNAKMRTWKHPFHRLKFKVYMSSAGVDDEHRADAQLFQRLTD
                     LIANIPNHGKHRLLAFDKNYHPDGSVNLAKTEFYVPNEYVFKLAEQHPDMFVPNISVN
                     PNRADAIAELEKWAQRGARVVKWLPNAMGIDPSDPRCDPFYQKMKQLDLILLSHGGEE
                     KAVEAKEDQKLGNPLLLRRPLDHGVKVIVAHCAGLGTNEDLDSRDRRQVHNFDLFLRL
                     MGDKRYEGLVFGEVSAMTQFNRLGKPLTTILEREDLQGRVVNGSDYPLPAINVLIRTR
                     PLVKYGYITPAEGDSLREIYRCNPLLFDFVLKRTLKRPGSDRRLPAAVFMTNPVLGV"
     gene            complement(2437..2892)
                     /locus_tag="VFO72_01965"
     CDS             complement(2437..2892)
                     /locus_tag="VFO72_01965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9478081.1"
                     /translation="MQHQATNPTFVEEIQRSFARQTVMTLIGGELTRVEAGVIEITLP
                     YRSDLTQQHGYVHAGVITTIADSACGYAAYTLMPAGSDVLAVEFKVNLLRPAKGDTFV
                     ARAEVIKSGRTLTVVRADVHALARGEQRELVAIMQGTMMRLKRPSPNNS"
     gene            complement(2873..>3528)
                     /gene="queG"
                     /locus_tag="VFO72_01970"
     CDS             complement(2873..>3528)
                     /gene="queG"
                     /locus_tag="VFO72_01970"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET9478082.1"
                     /translation="YHDVLKTKLESLLAWIREQDPDAAGKVCVDIQPTMDKAWAVRAG
                     LGWLGKHTNVITPEFGSWIFIGELLLNLDLDADADRFEDHCGTCTLCIDACPTQAIVE
                     PYVVDSNKCISYATIELRAPELPDAIQHGLSGWLYGCDICQDVCPWNRFEQKTGETPF
                     EPREGNINADLEEILELTPETYAARFRRSAMKRAKISGLQRNARALLSADSHATSSN"
BASE COUNT          767 a          978 c          960 g          823 t
ORIGIN      
        1 gaatgcgcgg ccaaacttca ttcgcctgct gtagtcgcgt agtgaggaaa agatttcatc
       61 tcccgcatat ccagggagag cgccggtgag aggcataagg actatttctc cgtttactaa
      121 ttctgcttta ccttcgacct tgtagaggtc gtcgattgtg gcttcgactt tggtgctcat
      181 agtcgtttcc tttaaccgcc gatgaagctc atggtgcgtg agggcgccac aaactctgga
      241 tcgttgatga agtgacgcgg ttcctttttc aagataacgg attcaatgta gtccacgatc
      301 tctgcacgcg aggcgccgct gcggaccacg tcgcgcaaag agtgttcgac tgttgaaaac
      361 aaacacgtgc gaatctgacc atcagccgtc agacgaattc gcgaacacgc gccgcagaac
      421 ggttctgtaa cgggcgcaat gataccgatt tcgcccggcg cgccgtccgc aaagcgataa
      481 cgtgcggaag tctcagaacc tctcttaata gcgagcttga tcagtggata tcgttcattt
      541 attcggtctt tgatctgttg tccggaaact acgtcagcgc gtgaccattc atgcccggaa
      601 tccagcggca tgaactcaat aaaacgcatc ttcacatcgt attcgcgcgc aaacgccgcg
      661 aagtccgcga cctcgtcttc attgtgcccg cgcacgatca cggcgttgat cttgatcgga
      721 tcgagtcccg cgtctttagc tgctgcgatt ccggcgagca ctttatccaa cacgtccacg
      781 cccgtcatcc gtttgaaaac atcccgtttc aaactgtcga gactgatcgt cacgcgatcc
      841 agcccggcgt ctttcagcgc gtgcgcacgt tcgggaagaa agtagccatt ggtcgtgagc
      901 gcgagatcga cgagtcgcgg ctcagcggtc ttcaaccgcg acagtttgcg gattatcgtc
      961 tcgatatcgc gacgcagcat gggctcgccg ccggtcagac gaatcttctc gatgcccaga
     1021 cttacgaaga tctcgcaaac gtaatcgatc tcttcaaacg agagcatctg ttccttgggc
     1081 gcaatcggcg gttcgccatg tggcagacag taaaagcagc gaaagttgca gcggtcagtt
     1141 agcgacacac gcaggtcgcg aatggctcga ccgtaggaat cccgcagtac cgtattttga
     1201 agactatctt tcacgcgcgt tcatgatacc gcgtgccgat cccgcgtcaa agcagggcca
     1261 cttacacgcc gagaactggg ttcgtcataa agacggcggc gggcagacgt ctgtcggaac
     1321 caggacgttt gagcgtgcgt ttcagcacga agtcgaaaag cagcgggttg caccggtaaa
     1381 tttctctcag cgaatcacct tcggctggtg tgatgtagcc atacttcacg agcggccggg
     1441 tgcgaatgag gacgttgatc gcgggtaatg gataatcgct gccgttaacc acacgccctt
     1501 ggagatcttc gcgctcgaga atcgtcgtca gcggcttgcc caggcgattg aactgcgtca
     1561 tcgccgatac ttcaccaaag acgagtcctt cataacgttt gtcgcccatc aatcgcaaaa
     1621 agagatcgaa gttgtgaacc tgccgccgat cgcgactgtc gagatcttcg tttgttccca
     1681 acccggcgca atgggcgacg atcaccttca caccatgatc gagtggccgc cgcaacagca
     1741 gcgggtttcc gagtttttga tcttccttcg cttcgactgc tttctcttca ccgccgtgac
     1801 tcaacagaat cagatcgagc tgcttcatct tctggtaaaa cggatcgcaa cgcggatcgg
     1861 aagggtctat gcccatcgca tttggcagcc acttcaccac gcgcgcgcca cgttgcgccc
     1921 acttttccag ctccgcgatc gcgtccgcgc ggttagggtt gaccgaaatg tttgggacaa
     1981 acatgtccgg gtgttgctcg gccagtttga atacgtactc gttcggaacg tagaactcag
     2041 ttttggctaa atttaccgag ccatcagggt gatagttctt gtcaaacgcc agcagccggt
     2101 gcttgccgtg attaggaatg ttggcgatca ggtctgtgag gcgttgaaac aattgcgcgt
     2161 cagcgcggtg ctcgtcgtca acgccggccg agctcatgta gaccttaaac ttcagccggt
     2221 gaaacgggtg tttccacgtc ctcatcttcg cgtttacgaa agccctgctt ccgttcgacc
     2281 cgagaccggc gatgtgtacg tgatgatcta cgaggcgttt cggatcgatg tcatcaaaag
     2341 cgcgcttgat cagatcggag gcgcgttgag tgatcttttc ggtgagctct tgcggagcgt
     2401 gattaaaagc gccgccgatg gaatgaatga gacacatcag gagttgttcg gtgatggtcg
     2461 ttttaagcgc atcatcgtac cttgcattat cgcgacaagt tcgcgctgct cgccgcgagc
     2521 tagagcatgg acgtccgccc tgacgacagt cagcgtccgg cctgatttga ttacctccgc
     2581 ccgcgcgaca aaagtatcac ctttcgcagg ccgcaacaga ttcactttga attcaacagc
     2641 caggacatca gaacccgctg gcatcagtgt ataggctgca tagccgcacg cggaatcagc
     2701 aatagtggtg atgacaccgg cgtgcacata gccgtgttgt tgagttaagt cactgcgata
     2761 cggcagcgtg atttcaataa ctccagcttc gacgcgggtc aattcaccac cgatgagggt
     2821 cattacggtt tgcctggcaa agctccgttg gatctcttcg acgaacgttg gattagttgc
     2881 ttgatgttgc atgggagtca gcagataaca gcgcgcgggc gttgcgctgc aagccggaaa
     2941 tcttggcgcg tttcatggcg ctgcggcgaa agcgcgcggc gtaggtttcg ggtgtgagtt
     3001 ccaggatctc ttccaaatcg gcgttaatat tgccctcgcg cggctcgaat ggcgtttcac
     3061 ctgtcttctg ctcgaagcga ttccagggac aaacgtcctg acaaatatcg cagccgtaca
     3121 accagcctga aaggccgtgc tgaattgcat ccggcagctc cggcgcgcgc aattcaatcg
     3181 ttgcgtatga aatgcacttg ttggaatcga cgacgtacgg ttcgacgatc gcttgcgtcg
     3241 gacacgcatc gatgcatagc gtgcacgtcc cgcaatgatc ctcgaaacga tcagcgtcag
     3301 catccagatc gaggttcaac aacaactcgc cgatgaagat ccacgaacca aactcgggcg
     3361 tgatcacgtt ggtgtgtttg ccgagccaac cgagcccggc acgcacggcc cacgccttgt
     3421 ccatcgtcgg ttgaatgtcc acgcagactt ttccggctgc atccggatcc tgttcgcgta
     3481 tccatgcaag cagcgattcg agttttgtct ttaatacgtc gtgatagt
//
LOCUS       DASTEY010000116         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1024448, whole genome shotgun sequence.
ACCESSION   DASTEY010000116 DASTEY010000000
VERSION     DASTEY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024448"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..437
                     /locus_tag="VFO72_01975"
     CDS             228..437
                     /locus_tag="VFO72_01975"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HET9478083.1"
                     /translation="MKLRPVVSEMLIAAGYDRRSRILEVIFRTGETYRYKGVPSSRYE
                     GLMSADSKGKYMHRHIIGHYDYERV"
     gene            488..2350
                     /locus_tag="VFO72_01980"
     CDS             488..2350
                     /locus_tag="VFO72_01980"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9478084.1"
                     /translation="MRFVQTCLFLVVFLFPQLTHAQKRVATGTVPPPQFTDPQRKQKL
                     AAAFPELEKLFKTFMERSQAPGAVFGVIVDGELVWVKAAGVREKTNDAVVTPETVFRI
                     ASMTKSFTAMAVLKLRDDGKLSLDDPVAKYVPELANLPYPTSDSQALTVRHLLTHSEG
                     FPEDNPWGDRQLAQSDATMREWLRAGIPFSTSPGTAFEYSNYGFAILGQIVAKASGRP
                     YADYVRDNILRPLGMNASTFEMSSVPREQIALGYRREDNKYKPEPILAHGSFGAMGGL
                     WTNAHDLARYVAFLMSAFPPRDEPERGPIKRSSAREMQQPWRPSPASAFRPTVDAPLQ
                     LGVSAYGYGLGISQDCRFGHVVGHGGGLPGYGSLMRWLPEYGVGLIGMSNQTYQGFNG
                     LFNDAISALHRTGALQPRVVQPSPALLTAQSDVSQLIVKWDDSLANRIAADNLFLDVA
                     AEVRSSRWKALAEQHGVCRPGSSIKPENALRGEWRMMCDRGWLDVYITLAPTMPPKVQ
                     LISINPVMPPDAEMTKAAETIVKLLSSWDAKTVEALVAPGFDVERMRRQVTAAAAWGS
                     CKVGEPLSGNGTRNSAIRLTCDNGPIAARIALDPNTHKLTNLDLVPLREQRCVP"
     gene            complement(2411..>2678)
                     /locus_tag="VFO72_01985"
     CDS             complement(2411..>2678)
                     /locus_tag="VFO72_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478085.1"
                     /translation="YRGKPCVFGHTPTPLLPLRGRLGRHGIYISHSAIGLDTGYNHQS
                     PLSCISLPDFNLYQTYADGREETYQITSFIPETLREMQRKAGLG"
BASE COUNT          592 a          745 c          765 g          576 t
ORIGIN      
        1 gacgtgcgtg agtacgccgc gcagaaggag gtcgacaccg aagcggcttt gaccctcggg
       61 atgaaggaaa aagcgaagga atttgtcgat accggcggcg agatctatcg aaaatagccc
      121 catttcccgc ataaacctca caaatttcta atgcctgcca actgcctctt gttttgatcc
      181 gcggcagatg tgcgaaaata cacacagcat tctggaggcg gaaagtaatg aagttaaggc
      241 cagtagtttc ggaaatgctg attgcggcag gctacgatcg caggtcgcgc atcctggagg
      301 tcatcttcag gactggtgag acataccgtt acaaaggagt gccttcgtct cggtacgagg
      361 gattaatgag tgcggattcc aaagggaagt acatgcaccg gcacatcatc ggtcactacg
      421 actacgagcg tgtttagcca cagattacac ggattcatcc acggatttct gttacattct
      481 tctgactatg cgcttcgtcc agacttgttt gtttctcgtc gtattccttt ttcctcaact
      541 aacacacgca caaaaacgag tcgctaccgg taccgttccg ccgccacagt tcaccgatcc
      601 gcaacgcaaa cagaaactcg ccgcagcgtt tcccgagctt gagaaattgt tcaagacttt
      661 catggaacgt tcgcaagcgc cgggcgcggt gttcggcgtg atcgtcgatg gcgaactcgt
      721 ctgggtaaaa gctgccgggg tgcgtgagaa gaccaacgat gcggtggtta cgccggaaac
      781 agtctttcgc atcgcgtcga tgacgaaaag cttcaccgcg atggcggtgc tgaagctgcg
      841 cgacgacggc aagctttcgc tggacgaccc agtggcgaag tacgtgccgg aactcgcgaa
      901 cttgccttat cccaccagcg attcccaggc actcactgta cgtcatctgc tgactcattc
      961 cgaaggtttt cccgaagaca atccctgggg cgatcggcag ttggcccaaa gcgacgctac
     1021 gatgcgcgaa tggttgcgcg cgggcattcc gttttcaact tcacccggca cggctttcga
     1081 atattcaaac tacggcttcg ccattctcgg ccagatcgtc gccaaagcct ccggccgtcc
     1141 ttacgccgac tacgtacgcg acaacatcct gcgtccactg ggaatgaacg cgtccacgtt
     1201 cgaaatgtcc tcggtgccgc gcgagcagat agcgctcggt tatcgtcgtg aagacaacaa
     1261 atacaaaccc gagcccatac tcgcgcacgg ttcgttcggc gcaatgggcg ggctttggac
     1321 caacgcacac gacctggcgc gctatgtcgc gtttctcatg tcggcgtttc cgccgcgcga
     1381 tgagcctgag cgtggtccga tcaagagaag cagcgcgcgc gagatgcagc aaccgtggcg
     1441 tccgtcaccc gcaagcgctt tccggccgac tgtcgatgcg ccgctccaac tcggagtttc
     1501 ggcgtatggt tatggactcg gtatttctca ggattgtcgc tttggtcatg tggttgggca
     1561 cggcggtggt ttgccgggtt acggttcact gatgcgctgg ttgccggaat acggcgtggg
     1621 actcattggc atgagtaacc agacgtacca gggattcaac ggtcttttca acgatgcgat
     1681 atcggcgctg catcgcacgg gcgcgttgca gccgagagtc gtgcaaccat cgcctgcctt
     1741 actaactgcg caatcagatg tttcgcagtt gatcgtcaag tgggacgata gtctcgcgaa
     1801 tcgcatcgcc gcggataatc tgttcctgga cgttgcggca gaagtacgct caagccgttg
     1861 gaaagcgttg gcggagcaac atggtgtttg ccgtcccggt agttcgatca aaccggagaa
     1921 tgcgttgcgc ggcgagtggc ggatgatgtg cgatcgcggt tggctcgatg tttacatcac
     1981 gctcgcgcca acgatgccac cgaaagttca actgatcagc atcaaccccg taatgcctcc
     2041 cgacgccgag atgaccaaag ccgccgagac aatcgtcaaa cttctttcat cctgggacgc
     2101 caagaccgtc gaagctctcg tggcgcctgg gttcgatgtt gaacggatgc gccgtcaggt
     2161 gacagcagca gcggcctggg ggagttgcaa agttggcgag cccctgagtg gaaacggcac
     2221 ccgcaacagc gctattcgat taacgtgcga caacggccca atcgccgcgc gcatcgccct
     2281 cgatccaaac acacacaagc taacaaatct ggatctggtc cctctgcgcg aacaacgctg
     2341 tgtaccataa agaagtcgca gatgaacgcg gatgacacgg ataaagatag ctatccgtct
     2401 tcatctgtgt ttatccgagg ccggctttgc gttgcatctc tcggagtgtt tcggggatga
     2461 aggaggtgat ttgataggtt tcttcgcggc cgtccgcgta ggtctgataa agattgaaat
     2521 cgggcagcga aatgcagctt agtggcgact ggtggttgta acccgtatcg agtccgatcg
     2581 cgctgtgcga aatgtaaatc ccgtgacgac ccaaccgtcc gcgcagcggc aacaacggcg
     2641 tcggcgtatg tccaaaaacg cacggcttgc cgcggtag
//
LOCUS       DASTEY010000117         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1271785, whole genome shotgun sequence.
ACCESSION   DASTEY010000117 DASTEY010000000
VERSION     DASTEY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271785"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VFO72_01990"
     CDS             <1..362
                     /locus_tag="VFO72_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478086.1"
                     /translation="IKDLAASFQHTVVQSLVKTMERVAEENYPRTLIIAGGVACNGKL
                     REVAREMAGRLGLPVYFPSPHLSTDNAAMIAAAGTVKLKAGQRDSLDLNADVTLRLQN
                     IDLAEEAAKRAGARYRL"
     gene            complement(482..1618)
                     /locus_tag="VFO72_01995"
     CDS             complement(482..1618)
                     /locus_tag="VFO72_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HET9478087.1"
                     /translation="MNNVLLLVVMVCAALTARAQDPLAQWKNFDFGTNAIKQAHIQDL
                     ELYQLKFLRGLVFGRHGRVFKDADIKSFLEEQPWYQPDPEFNNSMLNDVERRNLDVIR
                     MAEAAKHESIQPGDMRNWRNRQIPARKLGKHSGAEWKVLLAEVEAIHGKRFDHEPWLQ
                     QYFEERYWYFPNEKYDAKKLSAIERKNLEILSGAQKKQRRVALLPGDMELFENKTISE
                     QMLQGLSLHELRLLRNEIYARHGRMFRAEWLQQYFYLQPWYTPDENFKDEQLSGNDKL
                     NVETIVKYENRIHQELSTKPIARSLLEGLFLEDASQMRQEIYARHGKVFKEPWLQKYF
                     SSFDWYNANPNFTEADLTEVEKKNVATIAAYEKRAVTAMSTIEG"
     gene            complement(1652..2770)
                     /gene="dnaJ"
                     /locus_tag="VFO72_02000"
     CDS             complement(1652..2770)
                     /gene="dnaJ"
                     /locus_tag="VFO72_02000"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9478088.1"
                     /translation="MSKRDYYEVLKVSRTATDQEIKSAYRRLAVMYHPDKNPNDASAE
                     EKFKEAAEAYSVLSDPEQRRRYDRFGHAGVSSSAGAGNWGAPGFGGIEDILGDLFGFG
                     DVFGGARGGARRTTAQRGADLRYDLEITLEEAAKGMTAQLRIPRLETCDTCKGSGAKA
                     GTQPESCSSCGGTGQVRYQQGFFSVARPCHVCRGAGRVVKNPCETCHGAGRVEREKQM
                     EVKIPAGVETGSRLRVSGEGESGTQGGAPGDLYVVIHVAAHDQFERQGSNLYEAVPIT
                     FAQAALGADVMVKTLDGEEKLKVPMGTQTGTVFRLRGKGMPALGGRGRGDLFVSVTVM
                     TPTSLTREQRRLLEQLAEVENKDLEDKGLVDKVRDIFG"
BASE COUNT          569 a          891 c          751 g          672 t
ORIGIN      
        1 agatcaaaga cctggcggcc agttttcaac acacagtggt gcagtcgttg gtcaaaacga
       61 tggaacgagt ggctgaagaa aattacccgc gaactttgat catcgccggc ggcgttgcct
      121 gcaatggcaa actgcgcgaa gtcgcgcgtg agatggccgg ccggctgggt ctgccggtct
      181 acttcccttc accacatctt tctaccgaca atgccgcgat gatcgccgcc gccggtactg
      241 tgaaactgaa agctggtcaa cgcgacagtt tagacctgaa cgccgatgtt actttgcgcc
      301 tgcaaaacat cgacctcgca gaagaagccg ctaaacgcgc aggcgctcgc taccggctat
      361 aagccacaga cgaacaccga gagcacaaat cagcttgagg ttctgatctg taatctctgt
      421 gtctgtctgt gcctctttag ttgtggctgg cctcgtcacg cgagacctga ggtgggcctt
      481 atcaaccctc tatcgtgctc atcgcggtga cggcacgctt ttcatacgcc gcaattgtgg
      541 cgacgttctt cttctccacc tccgttaaat ccgcttcggt gaagtttggg ttggcattgt
      601 accagtcgaa actggaaaag tatttttgca accacggctc cttgaaaacc ttgccgtgac
      661 gcgcatagat ctcctgccgc atctggctcg cgtcttcgag aaacaatcct tccaaaagcg
      721 agcgcgcgat cggtttcgta ctcaactcct ggtgaatgcg attctcatac ttaacgatcg
      781 tttccacgtt caacttgtcg ttgccggaaa gctgttcgtc tttgaagttt tcgtcgggcg
      841 tgtaccacgg ttgcaagtag aaatactgct gcaaccactc agcgcgaaac atcctgccat
      901 gacgtgcgta gatctcgttc cgcagcagtc gcaactcgtg caaactcagt ccctgcaaca
      961 tctgctcaga gatcgtcttg ttttcgaaaa gctccatgtc gcccggcaag agcgccacgc
     1021 gccgttgttt cttctgcgcg ccgctaagta tttcgaggtt cttccgctcg atggctgaga
     1081 gcttcttcgc gtcgtacttc tcgttcggga aataccagta acgttcttcg aagtactgtt
     1141 gcagccacgg ttcgtggtcg aaacgcttgc cgtgaatggc ttcaacttcc gccagcaata
     1201 ccttccattc agcgccgctg tgcttgccga gttttcttgc gggtatttgg cgattgcgcc
     1261 agttacgcat gtcgccgggt tgaatgctct cgtgtttggc cgcctcagcc atgcgaatca
     1321 cgtcgagatt gcgccgctcg acgtcattga gcatcgagtt gttaaactcg gggtcaggtt
     1381 ggtaccacgg ctgctcttcg agaaacgact taatgtctgc atctttgaag acccgtccat
     1441 gacgtccaaa tacgagcccg cgcagaaact tcaactggta gagctcgagg tcctgaatat
     1501 gcgcttgctt gatcgcgttg gtgccgaagt cgaaattctt ccactgggcc agcggatcct
     1561 gcgcgcgggc cgtaagcgcc gcacacacca tcactaccaa caacagaacg ttgttcacaa
     1621 attgtcgggg ggttaagctc atcgcagcct ttcagccgaa aatgtctcgc actttgtcga
     1681 ccagaccttt gtcttccaag tctttgtttt caacttcggc caactgttcc agcagtcgcc
     1741 tttgttctcg cgtcagcgaa gtcggggtca tcacagtcac ggaaacaaac agatcgcctc
     1801 gaccacgtcc accgagtgcg ggcatccctt tcccgcgcag gcgaaacacc gtaccggttt
     1861 gcgtgcccat cggaaccttg agtttctctt cgccatcgag cgtcttcacc atgacgtctg
     1921 cacccaacgc ggcctgcgcg aacgtgatcg gcacggcctc gtaaaggttg ctaccttgtc
     1981 gttcaaattg gtcgtgcgcg gcaacgtgga taaccacgta aagatcgccc ggtgcgccac
     2041 cctgcgtgcc ggattcgcct tcacctgaaa cgcgtaatct ggaaccggtt tcgacccccg
     2101 cgggaatttt tacttccatc tgcttctcgc gctcgacgcg gcccgctccg tgacacgtct
     2161 cacacggatt cttaactaca cgacccgcgc cacgacacac gtgacacggc cgcgccacgg
     2221 aaaagaaccc ctgctggtaa cgcacctgtc cggtgccgcc gcaactggag caactctcag
     2281 gttgcgtgcc cgccttcgcg cccgaacctt tacacgtgtc gcaggtctcg agacgcggaa
     2341 tgcgcaactg cgcagtcatt cccttcgcgg cctcttcgag cgtgatctcg aggtcgtagc
     2401 gaaggtcggc gccacgttgg gctgtggttc gccgggcgcc gccgcgggcg ccgccgaaca
     2461 cgtcgccaaa tccaaacagg tcgccgagta tgtcctcgat accgccaaac ccgggcgcgc
     2521 cccagtttcc cgcgccggcg ctgctggaaa ctcccgcgtg accaaaccgg tcgtaacgcc
     2581 tgcgctgttc gggatcagac aacacgctgt aggcttcggc ggcttccttg aacttctctt
     2641 ccgccgacgc gtcgttgggg ttcttgtcag ggtgatacat caccgccagc cgccggtacg
     2701 cgctcttgat ctcctgatcg gtcgcggtgc ggcttacctt caatacttcg taataatcac
     2761 gtttgctcat tcagccttgt aaaacctcaa tgctgggacc gcgggcgtct cgcccgcaaa
     2821 gtgccgacag cgtctttttg cattagccag cgggcgagac gcccgcgatc ccaggtttaa
     2881 acc
//
LOCUS       DASTEY010000118         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_563188, whole genome shotgun sequence.
ACCESSION   DASTEY010000118 DASTEY010000000
VERSION     DASTEY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563188"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VFO72_02005"
     CDS             complement(<1..1434)
                     /locus_tag="VFO72_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478089.1"
                     /translation="MLKDIRYGARMLTKNPGVTLVAVITLALGIGANTAIFSGVNAFL
                     MRPLPVPRPNELVRQMEVTEDRELTDELSYPDFLDYRAQSTSFVGLAAEDMTPAAIDT
                     EDQNDVIWGQVVSANYFEVVQVTPVLGRTFAPDEDRIVGANPVLVLSHSLWQRRFAGD
                     PGIVGKTVRLNNGQYEVIGVAPESFTGTKFALAMDFWAPISMAEDLRRSPKLLEDRGS
                     HWMNVIGRLKPGVSLAQASAELAAIANRINQAYPDNRVHNTQSLVMTELEGRFEDMGG
                     VFKSGGAIAMAIVGLILLIACANVANLMLARAAARRKEIGIRLALGASRARLIRQLLT
                     ESLLLSVLGGALGLLLAVWVTKLMEGFIPILEYTIIKDFFALDSRALLFTLAVSLATG
                     LIFGLAPAWHSSNPDVVPVLKGLPEVPQRGRMGRFTLRNSLVVAQVALSLMVLVCGGL
                     FIKSFRNAQTMDPGFTNPNGLIVSLSPQ"
     gene            complement(1458..>2940)
                     /locus_tag="VFO72_02010"
     CDS             complement(1458..>2940)
                     /locus_tag="VFO72_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9478090.1"
                     /translation="ASRWRIARQLLTESILLSLIGGVLGVLVAWWGTKALVALSPPAL
                     IDLQRVSVNLPVLGFTLALSLITGIVFGLAPALEATRFDLQGALKEGGKNIGGAAGSS
                     YLRNVFVVTQVALALVLLVGAGLLFRSFTRLQSVDPGFNAQNLLTVRVSLPFARYDTE
                     QKRIDFFQQAITQMKSLPGVQSVGAINTAPFTGLYSGTTVEIAGAPPLPPEQELKTGV
                     CVTDANYFQTMQIPLTRGRLYTQQETTEMRHVVVVNETFVKKNFDGQDPLGKRLVIYM
                     KQENVPTEVIGVVADHKHLGLDVAVEPMVYWPHAELPFGAMTLMLRTTNDPNAVAPAA
                     RGVIRAVDPLQPIGEISTMESLLSVSVSRARFSASLLTVFSIVALVMAAVGIYGVMSY
                     SVLQRTHEIGVRMALGAQRFDVLKLVVKKGIALGVVGVVVGLAVSFLVTRLIATLLFE
                     VTATDKATFAVVSIGLFLVTLLACYVPARRATKVDPMKALRYE"
BASE COUNT          701 a          907 c          770 g          562 t
ORIGIN      
        1 ctgcgggctc agcgacacga tcaatccatt gggattagta aaaccgggat ccatcgtctg
       61 ggcgttgcga aaactcttga tgaagaggcc gccgcagacc aggaccataa gcgagagtgc
      121 gacctgcgcc acgacgagac tgttgcgcaa agtgaaacgg cccatacggc ctctctgcgg
      181 cacttccggc aggcctttca acaccggcac cacgtccggg ttcgacgagt gccacgccgg
      241 cgcaagtccg aagatcaaac cggttgccaa cgaaacggcc agcgtgaaca gcaacgcacg
      301 cgagtcgaga gcaaaaaagt ctttgatgat tgtgtattcg aggatcggaa tgaaaccttc
      361 catcagcttc gtcacccaaa ccgcgagcaa caatccaagc gcgccaccca gcactgaaag
      421 cagtagactc tccgtcaaaa gttgacgaat caaacgcgcg cgactcgcgc ccagcgccag
      481 acgaatacca atctccttgc gacgcgctgc tgcacgcgcg agcatgaggt tggcgacatt
      541 cgcgcaggcg atcagcagta tcaaacccac gatcgccata gcgatcgcgc caccgctctt
      601 gaagacgccg cccatgtctt cgaaccgccc ctcgagctcg gtcatcacca gcgattgcgt
      661 gttatgcaca cgattgtcgg gatacgcctg attgattcgg ttcgcgattg cggccaattc
      721 ggctgacgcc tgcgccaatg aaactccagg cttcaaacgg ccgatgacat tcatccagtg
      781 actgccgcga tcctcgagta acttcggcga gcgacggaga tcttccgcca tcgaaatggg
      841 ggcccagaag tccatcgcga gcgcaaactt cgttccggtg aaagactcag gcgcgacacc
      901 aatcacttcg tactgaccgt tgttcaggcg aactgtcttg ccaacaatgc ctggatcgcc
      961 cgcgaaacgg cgctgccaga gactgtgact aagaaccaaa accggattcg cgccaacgat
     1021 cctatcttcg tcgggcgcga acgtgcggcc gagcaccggc gtaacctgga ctacttcgaa
     1081 gtagttcgca gaaactacct gaccccagat gacgtcgttc tgatcttcag tgtcgatcgc
     1141 agccggagtc atgtcttccg ccgcgagccc cacgaatgaa gtgctttgcg cgcgatagtc
     1201 gagaaagtct ggatacgaca gttcgtccgt gagttcacgg tcctccgtca cctccatctg
     1261 gcggaccagc tcattcggcc gcggcaccgg caacgggcgc attagaaatg cattcacgcc
     1321 actaaagatc gccgtgttgg cgccaatacc gagagccagc gtgattaccg ccacgagggt
     1381 gacacccgga ttcttcgtga gcatccgagc tccataacga atatctttaa gcatatctgt
     1441 gtagtctctg gtccatatca ctcgtaccta agtgccttca tcggatcgac tttcgtcgct
     1501 cgacgcgccg gcacgtaaca cgccagcagc gtgactaaga acagtccgat cgaaacgacg
     1561 gcaaacgtcg ccttgtcagt cgccgtcacc tcaaacagca atgtagcaat gagcctcgtc
     1621 accaaaaacg acaccgccaa accaacgacg acgccaacca cgcccaacgc gattcccttc
     1681 ttcaccacca gtttcagcac gtcaaaacgt tgcgcgccaa gcgccatccg gacaccgatc
     1741 tcatgcgtac gttgcaaaac ggagtaggac atcacaccat agatcccaac tgccgccatc
     1801 acgagcgcga caatcgaaaa cacggtcagc aacgacgcac tgaaccgcgc ccgtgacact
     1861 gaaacggaaa gcagactttc catcgtgctg atctctccga tcggctggag tggatcgacc
     1921 gcgcgaatca cgccgcgtgc agcaggtgca acggcgttcg gatcgttggt cgttcgcagc
     1981 atcaacgtca ttgcgccgaa aggcagttca gcatgaggcc agtagaccat cggctcgacg
     2041 gccacatcga gacccagatg tttgtgatcg gccacgacgc ctatgacctc cgtcggcacg
     2101 ttttcctgct tcatgtaaat cacgagccgt ttgcccaggg gatcctggcc gtcgaagttc
     2161 ttcttcacaa acgtttcgtt caccacgacg acgtgacgca tctctgtggt ttcctgttgc
     2221 gtgtaaaggc ggccacgcgt caatggaatc tgcatcgtct gaaagtagtt ggcgtcggta
     2281 acgcatacgc ccgtttttag ttcttgttcc ggcggcaatg gaggcgcgcc ggcgatttca
     2341 acagtcgttc cgctgtagag tccggtgaat ggagcagtgt tgattgcgcc tacagattgc
     2401 acgcccggta gcgatttcat ctgcgtgatc gcctgctgga aaaagtcaat tcgcttctgt
     2461 tcggtgtcgt acctggcgaa tggcagactg acgcgcacag tcagaagatt ttgcgcgttg
     2521 aatccgggat cgaccgattg caaacgcgtg aaactcctga agagcagccc ggcgccgacc
     2581 agcaacacga gcgcaagcgc cacttgtgta actacaaaaa catttcgcag ataagaactg
     2641 ccggcagcgc cgcctatatt ctttccacct tctttcagcg ccccttgaag atcgaagcgc
     2701 gtagcttcca gcgcgggagc taacccgaaa acgattccag tgatcagcga caacgccaac
     2761 gtgaatccca gaaccggcag attgacgcta actctttgga gatcgattaa ggcgggggga
     2821 cttagcgcaa ccagcgcttt cgttccccac catgcaacga gaacaccgag cacaccgccg
     2881 atcagcgaca gcaagatgct ctcagtcagc aactgtcgcg cgatgcgcca gcggctggcg
//
LOCUS       DASTEY010000119         5753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1568338, whole genome shotgun sequence.
ACCESSION   DASTEY010000119 DASTEY010000000
VERSION     DASTEY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568338"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VFO72_02015"
     CDS             <1..96
                     /locus_tag="VFO72_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET9478091.1"
                     /translation="IRGTLDSVGVADRKQGRSKYGAKRPKAGAGK"
     gene            130..600
                     /gene="rpsG"
                     /locus_tag="VFO72_02020"
     CDS             130..600
                     /gene="rpsG"
                     /locus_tag="VFO72_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET9478092.1"
                     /translation="MPRRRVAERREVLPDPVYNSPLVTKFISGVMWEGKKSVAESIFY
                     DAMRQIGEKTGEEPLKVFKRAIDNVKPTVEVKSRRVGGSTYQVPIEVHQERRGALAIR
                     WIVSYARGRGEKTMTDRLAAEILDAANNRGNAVKKKDDTHRMADANKAFAHYKW"
     gene            693..2789
                     /gene="fusA"
                     /locus_tag="VFO72_02025"
     CDS             693..2789
                     /gene="fusA"
                     /locus_tag="VFO72_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9478093.1"
                     /translation="MAKHDLSKFRNIGIMAHIDAGKTTTTERILYYTGITHKIGEVHE
                     GTATMDWMEQEQERGITITSAATTCFWERNGVDHRINIIDTPGHVDFTMEVERSLRVL
                     DGAVAVFDGVAGVEPQSETVWRQADKYGVPRICFINKLDRAGASFDRSFESIITRLGA
                     NPVAVQIPIGLEDQLKGVVDLIAMKGLIWKDETKGAEYEVAEIPADLKDAAAAAREKL
                     IEAVASIDDDLMHKYLEGEEISEQEIRNALRAGTIGLKLVPVVTGSAFKNKGVQTLLD
                     AVVDYLPSPLDIPPVEGINPKSSQKEARPPEASAPFAGLVFKIMADKHLGQLSFVRIY
                     SGTIKAGSYAYNPIKDTKERVGRLMRMHANKREDIEVASAGEIIAIGGMKQVTTGDTI
                     CDENKPVVLEAMEFPAPVIRVAVEPKTRQDQDKLGVALNRLAQEDPSFNVSTDQETGQ
                     TIIAGMGELHLEIIVDRMKREFGVEANVGKPQVAYRETITKPAPGKEVYKKQTGGRGQ
                     YGHVEIEIEPAPGEGFVFVNEITGGAIPKEYIKPVEHGIRDAMERGFLAGYEMVDIKV
                     RLVFGSYHEVDSDERSFHIAGSLAFQDAVKKAGPVLLEPIMRVEVVTPNDYTGAVNGD
                     LNSRRGRIESMEPRVGGAQVITAFVPLSEMFGYTTDLRSATQGRATSTMHFERYEQAP
                     KTIAEEVIARVQGVAK"
     gene            2873..4060
                     /gene="tuf"
                     /locus_tag="VFO72_02030"
     CDS             2873..4060
                     /gene="tuf"
                     /locus_tag="VFO72_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9478094.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAKNNPKVQ
                     FVAFDQIDKAPEEKARGITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAMVVCLNKVDAVDDPELLELVELEVRELLS
                     AYEFPGDDIPVIRCSALKALEGDPEWEKTVLELMDAVDSYIPTPERDIEKPFLMPVED
                     IFTIQGRGTVATGRVERGKVKVNEAVEIVGIRPTRNTVVTGVEMFKKLLDEGVAGDNV
                     GLLLRGVDRKDIERGQVIAKPGSITPHTKFKAEAYVLTKEEGGRHTPFFTGYRPQFYF
                     RTTDVTGVAHLPAGVEMVMPGDNVAMEIELIAPIAMEKGLRFAIREGGRTVGAGTVSE
                     VVE"
     gene            4152..4304
                     /gene="rpmG"
                     /locus_tag="VFO72_02035"
     CDS             4152..4304
                     /gene="rpmG"
                     /locus_tag="VFO72_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005918839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9478095.1"
                     /translation="MPRDNIVLQCNECKSRNYVTTKNKKTTPDRLEFSKFCRRCRKHT
                     GHKETK"
     gene            4397..4472
                     /locus_tag="VFO72_02040"
     tRNA            4397..4472
                     /locus_tag="VFO72_02040"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4431..4433,aa:Trp,seq:cca)
     gene            4487..4813
                     /gene="secE"
                     /locus_tag="VFO72_02045"
     CDS             4487..4813
                     /gene="secE"
                     /locus_tag="VFO72_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9478096.1"
                     /translation="MAETKNPEAPIVTPRTARFGDGPEGKATRRERGQSAPSRIGQFL
                     HDVRAELKRVSWPNATHVQNTTIITLIAVIFFAIYLFAVDQALSRLIIGLDWLLEKIA
                     GFLGLA"
     gene            4829..5389
                     /gene="nusG"
                     /locus_tag="VFO72_02050"
     CDS             4829..5389
                     /gene="nusG"
                     /locus_tag="VFO72_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET9478097.1"
                     /translation="MLQWFILHTYSGHEKKVRDSLKARIRDTDLQNEITEVLVPTETV
                     VEMRGNKRIESSRMFYPGYVLVQIDCDERGHIPDNVFHMIKSTPKVTGFVGGHNPTPL
                     TPQEVDQIVNQQAEAAEKPKPKFSYAAGETVRIKSGPFQSFTGKVEEVNEDKSTLKVT
                     VTIFGRSTPVELNVLDVEKVTFAEEE"
     gene            5392..>5753
                     /gene="rplK"
                     /locus_tag="VFO72_02055"
     CDS             5392..>5753
                     /gene="rplK"
                     /locus_tag="VFO72_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9478098.1"
                     /translation="MAKKITGYIKLQVPAGKANPAPPIGPALGQHGVNIMEFCKAFNA
                     KTAQMDPDLKVPVVITVYADRSFTFETKTPPAADLLKKAAAIPKGSGKPNREKVGTIS
                     RAKIEEIAKTKLPDLNTT"
BASE COUNT         1381 a         1427 c         1831 g         1114 t
ORIGIN      
        1 attcgcggca cgctcgattc ggtcggcgtg gcggatcgca agcagggacg ctcgaagtac
       61 ggagcgaaac ggccgaaggc aggcgccggc aaatagagat cagacgcctg gaaatagcga
      121 gagacgaata tgccaagacg acgagtagct gaacgacgcg aagtacttcc cgatccggtt
      181 tataacagcc cgctggtcac taagtttatc agcggcgtga tgtgggaagg caagaagagc
      241 gtagcggaaa gcatcttcta cgatgccatg cggcagatcg gcgagaaaac cggcgaggag
      301 ccgctgaaag ttttcaaacg agccatcgac aacgtgaaac ccacggtcga agttaagtca
      361 cggcgcgtcg gcggttcgac ctatcaggtt ccgattgaag ttcaccagga gcggcgcggt
      421 gccctggcta ttcgttggat tgtctcctac gcgcgggggc gcggtgaaaa aacgatgacc
      481 gatcgccttg cggcagagat tctcgacgcc gccaacaatc gcggcaacgc ggtgaagaag
      541 aaggacgaca cgcatcgtat ggcggacgcc aacaaggcat tcgctcacta taagtggtaa
      601 gagattttcg attttcgatt gccgatttaa gcgatgctcg gcaagtttga aagaaattgg
      661 caatctgcaa tcgaaaattg gcaatgaaag caatggctaa gcacgattta agtaagttca
      721 ggaatattgg catcatggcc cacatcgatg ccggtaagac caccaccacg gaacgtatcc
      781 tctattacac gggcatcacg cacaaaatcg gcgaggtgca cgagggtacg gcgacgatgg
      841 actggatgga acaggagcag gagcgcggta tcactatcac cagcgctgcg acaacctgtt
      901 tctgggagcg caatggtgtc gatcatcgca tcaatatcat cgacactccc ggtcacgtcg
      961 acttcacgat ggaagtggaa cgttcgcttc gtgtgctgga tggtgctgtc gctgtgtttg
     1021 acggcgtggc cggcgtggag ccgcagtcgg agactgtctg gcggcaagct gataaatatg
     1081 gcgtcccgcg aatctgtttt atcaacaagc tggatcgcgc gggcgcttcg tttgaccgct
     1141 cattcgagtc gatcattacc cgcctgggtg caaacccggt agccgtgcaa atcccgatag
     1201 gcctcgaaga tcaactcaaa ggcgtcgtcg atctgattgc gatgaagggg ctgatttgga
     1261 aagacgagac caagggcgct gagtacgaag tcgccgagat tcccgcagat ttgaaagacg
     1321 ctgccgcggc tgcgcgagaa aaattgatcg aagctgttgc gagcattgac gacgacttga
     1381 tgcacaagta tctcgagggt gaagagatct ccgagcagga gattcgcaac gctctccgcg
     1441 ctggtacgat tgggctgaaa ctcgtgccgg tagtcaccgg ttctgctttc aagaacaaag
     1501 gcgtgcagac gttgctcgat gcggtcgttg attatcttcc gtcaccgctc gacattccgc
     1561 cggtcgaagg catcaacccg aaatcaagtc agaaggaagc gcgtccaccc gaggcgtcgg
     1621 cgccgtttgc cggtctggtc ttcaagatca tggccgacaa gcacctgggc caactctcgt
     1681 tcgtgcgtat ttacagcggc acgatcaagg ccggttcgta cgcttacaac ccgatcaagg
     1741 acacgaagga acgcgtcggt cgcctgatgc gcatgcacgc taacaagcgt gaggacattg
     1801 aggtcgcgag cgcaggcgag atcattgcta tcggtggcat gaagcaggtc accaccggtg
     1861 acacgatctg cgacgagaac aagcctgttg ttcttgaagc gatggagttt cccgcgccgg
     1921 tgattcgtgt cgctgtcgaa ccgaagacgc ggcaggacca ggacaaactc ggcgtggctt
     1981 tgaaccgcct tgcgcaggaa gacccgtcgt tcaatgtttc gacggaccag gagactgggc
     2041 agacgatcat cgccggcatg ggcgaactgc acctggagat catcgtcgat cgcatgaagc
     2101 gcgagtttgg cgttgaagcc aatgtcggta agccgcaggt ggcgtatcgc gaaacgatta
     2161 ccaaacccgc gcccggcaaa gaggtctata agaaacaaac gggtggccgc ggccagtacg
     2221 gtcacgtcga gatcgagatc gagcccgctc cgggcgaggg tttcgtgttc gtcaacgaga
     2281 tcactggtgg tgcgattccg aaggaataca tcaagccggt ggagcacggg attcgcgacg
     2341 cgatggagcg cgggttcctg gccggctatg aaatggtgga catcaaggtg cggctcgtgt
     2401 tcggtagtta tcacgaagtc gactcggatg agcgttcgtt ccatatcgcc ggttcgctgg
     2461 cgtttcagga tgcggtgaag aaggctggtc cagtgttgct cgagccgatc atgcgcgtgg
     2521 aggtcgttac tccaaacgac tacactggcg cggtaaatgg cgatctaaat tcgcgtcgcg
     2581 gccgcattga aagcatggaa ccgcgcgtcg gaggcgcgca ggtaatcacg gccttcgtgc
     2641 cgctttcaga gatgtttggt tacacgacgg acttacggtc ggcgacccag ggccgcgcga
     2701 cttcgaccat gcatttcgag cgttacgagc aggcgccgaa gaccattgcg gaagaagtta
     2761 ttgcacgggt tcagggagta gcaaagtagg acaggcattc tgcctgtcta ttgccgattt
     2821 tcgattgtcg attgtcaatt gaaaaaacaa acaaagaggt tgggacaaac agatgtcgaa
     2881 ggaaaaattt gaccgttcga agccgcacgt gaacatcggg acgatagggc acgtggatca
     2941 cgggaagacg acgttgacgg cagcgatcac gaaggtgttg gcgaagaaca atccgaaggt
     3001 gcagtttgtg gctttcgacc agatcgacaa ggcgccggaa gagaaggcgc gcggcatcac
     3061 gatcgcgacg gcgcacgtgg agtatgagac ggccaaccgg cactacgcgc acgttgattg
     3121 tccgggacac gccgactacg tgaagaacat gatcacgggc gcggcgcaga tggacggcgc
     3181 gattctggtg gtggcggcca ccgacggccc gatgccgcag acgcgcgagc acattctgct
     3241 ggcgcggcag gtaggcgtac cggcgatggt ggtgtgcctg aacaaagtgg acgcggtcga
     3301 cgatccggaa ctgctggagt tggtggaact ggaagtgcgc gagttgttga gcgcatacga
     3361 gtttccgggc gacgatattc cggtgatcag gtgctcggcg ttgaaggctt tggaaggcga
     3421 tcccgaatgg gagaagacgg tgctggaatt gatggatgca gtggacagct acattccgac
     3481 gcccgaaaga gatattgaga agccgttttt gatgccggtg gaagacatct tcacgattca
     3541 ggggcggggc acggtggcca ctggccgcgt ggagcgcggc aaggtgaagg tgaacgaagc
     3601 ggtggagatt gtgggcatcc ggccgaccag gaacacggtg gtgacgggtg tggagatgtt
     3661 caagaagctg ctggacgaag gtgtggcggg cgacaacgtg ggtttgctgc tgcgtggggt
     3721 ggatcgcaag gacatcgagc gcgggcaggt gattgccaaa ccgggttcga tcacgccgca
     3781 cacgaagttc aaggctgaag cttacgtgtt gacgaaggaa gaaggtggac ggcacacgcc
     3841 gtttttcacc gggtaccggc cgcagttcta cttccgcacc acggacgtga cgggagtggc
     3901 gcacctgccg gcgggagtgg agatggtgat gccgggcgac aacgtggcga tggagatcga
     3961 gttgatcgcg ccgatcgcta tggagaaggg gttgcgcttc gcgatccgtg aaggcggccg
     4021 tacggtcgga gccggcacgg tttcggaagt agtggagtag aggcattgcc gattttcgat
     4081 tgccgattgc cgatttaaga aggcagtggc aagttgaaaa gaaatcggca atcgaaaatc
     4141 ggaaataagt tatgccacgc gacaatattg tgcttcagtg taacgaatgt aagagtcgca
     4201 actacgttac gactaagaat aagaagacaa cgcccgaccg gctggagttc agcaaattct
     4261 gccgtcgttg ccgcaagcac actggccaca aagaaacgaa gtaaagccat tgtcgatttt
     4321 cgattgccga ttgctgattg cggagaccgg cgtgcataca atcgaccatc gacaatcggc
     4381 aatcggcaat gcctgaaggg gtatagtgtc aacggttagc acgtcggtct ccaaaaccga
     4441 aagtccgggt tcgaatcctg gtacccctgc catttgaaat tgagttatgg ctgagacaaa
     4501 aaacccagaa gcaccgatcg tgacgccaag aaccgcgcgc tttggtgatg ggccggaagg
     4561 caaggcgacg cggcgcgagc gcgggcaaag tgctccgagc cgcatcggtc agtttttgca
     4621 cgacgtgcgg gcggagctga aacgtgtgtc ctggccgaac gcgactcacg tgcagaacac
     4681 caccatcatc acgctgatcg cagtgatctt cttcgcgatt tacttgttcg ccgtcgatca
     4741 ggctttgtcg cgactcatta ttggtctgga ttggttactg gaaaagatcg caggtttttt
     4801 gggcctcgcc tgaagaaaag agttctacat gcttcagtgg ttcattcttc atacgtactc
     4861 gggacacgag aaaaaggtgc gcgatagcct gaaagcgcgt atccgggaca ccgatctgca
     4921 aaacgagatc acagaggtcc tggtgcccac tgaaaccgtc gtggagatgc gcggcaacaa
     4981 gcgaatcgag tcgtcgcgca tgttttatcc cggttacgta ctggtgcaga ttgattgcga
     5041 cgagcgcggc catattccgg ataacgtgtt tcatatgatc aagtcgactc caaaggtgac
     5101 gggttttgtc ggtggtcaca atcccacgcc gctgacgccg caggaagtag accagatcgt
     5161 taatcagcag gccgaggccg cggagaagcc gaagccgaag ttttcgtatg cggcaggcga
     5221 gactgtgcgc atcaagtcgg ggccgttcca gagttttacc ggcaaggttg aagaggtgaa
     5281 tgaagacaag tcgacgctga aagtgacggt gacgatcttc ggccgttcga cgccggtgga
     5341 gttgaacgtc ctcgatgttg agaaagtgac gtttgcggaa gaagagtaaa gatggcaaag
     5401 aagattaccg gttacatcaa gctacaggtt cccgcgggca aggcgaatcc ggcgccgcca
     5461 atcggaccgg cgctcggtca gcacggcgtg aacatcatgg aattctgtaa ggcgttcaac
     5521 gccaagaccg cgcagatgga tcccgacctg aaagtgccgg tcgtgatcac ggtttacgcg
     5581 gatcgctcgt ttacgtttga aacgaagacg cctccggcgg ctgatttgct caaaaaggcg
     5641 gccgccattc cgaagggatc gggcaagccg aaccgcgaga aggtcggcac catttcgcgg
     5701 gcgaagatcg aagagatcgc caaaaccaaa ctgcctgact tgaacaccac gag
//
LOCUS       DASTEY010000120         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_760953, whole genome shotgun sequence.
ACCESSION   DASTEY010000120 DASTEY010000000
VERSION     DASTEY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760953"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFO72_02060"
     CDS             <1..186
                     /locus_tag="VFO72_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478099.1"
                     /translation="PRVQSPWLAGVNASHPSMERFGTEEATVSELVDFFQTEKACSLE
                     PGGKPCDHCAMCSSRGF"
     gene            complement(193..807)
                     /locus_tag="VFO72_02065"
     CDS             complement(193..807)
                     /locus_tag="VFO72_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9478100.1"
                     /translation="MKTRTVLILLAVAVISLGASGCGYNTLTTKQQNVKGKWANVETQ
                     LQRRADLINNLVESAKLAGIQEQEVFGTIAEARSRLLNATQQAPQGEGGDKSPEQKQA
                     VIDAANTFGGTIGRLLVLNEAYPQLQSNQNFLKLQDEVAGTENRIATARKDYNDATQD
                     YNTTRARFPTVISAKLFGFKEEPYFRAEEGAQQPPTINANSLRR"
     gene            complement(804..1547)
                     /locus_tag="VFO72_02070"
     CDS             complement(804..1547)
                     /locus_tag="VFO72_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478101.1"
                     /translation="MVKAVVQQALNGLVDDLRATHGENLACVVLYGPAVADDRNELPQ
                     DYNLLVALNRITPEDLRQAQAPMREWQRLGYPLPVYFTVEELSDAGDVFPIEFHQMAT
                     ARTVLYGHDPFEFVKLSDAHLRHQTEYELRSKLIQLRRHYIPASISIEKLCDLMSDSL
                     SSFAVLFRAALLVFGQEAPVTNVECVRATTKMLKLDITPFETIFEFRAGGRWPASEKE
                     ANELFAAYLFQIEQVVEAVDELERRGDRI"
     gene            complement(1582..2502)
                     /locus_tag="VFO72_02075"
     CDS             complement(1582..2502)
                     /locus_tag="VFO72_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9478102.1"
                     /translation="MRLKNSELRQRLGIGLLALLFLLLPGFAQAQSTPQSPVPLPVPF
                     TPIVDNANVIDAQTRERLESIFRNLKERGNIEYAVLTVPSTGEQDIFDFSLAVARGWG
                     IGPKEGEKAGFLLVVAINDRKYFTQISDHLEGDLPDGLVGQIQRERLVPAFRQGNYSK
                     GIYDTVQAYVATLAQNRGFSVEGIDQNYAYRAEPEGDIRIREIPGTKFSTICCGALVL
                     LAILIAVSLGSRGGPRGGHRGGWGGGWWQAALLASLLSNGGRSGRSRGGWGGGFGGGG
                     FGGGGGGGGGFGGGFGGGGSFGGGGAGGSW"
     gene            complement(2529..3311)
                     /locus_tag="VFO72_02080"
     CDS             complement(2529..3311)
                     /locus_tag="VFO72_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET9478103.1"
                     /translation="MTSILLFGLLAAGANVLGGLVLVKSGMHRWGERFLKYLVALGAG
                     FMLAAIFIEILPESISLWTSGARGESASEAVVGAMTLLLAGYLLIQFFEHTLAPHFHF
                     GAETHPESFMRRSAAYTAVGGLCIHTFFDGVSIASASLVNFRVGMLVFIAILLHKMPE
                     GFTVASIVLASGRSTQKALIATAAIGAATLAGVIAVALLDARMTDAVAYALPFSAGVT
                     LYVAASDLIPEVNHKEERNPTVSIVVFVGVALFYLLHKVIEG"
BASE COUNT          755 a         1025 c          911 g          706 t
ORIGIN      
        1 ccacgggttc agtcaccctg gctggccggc gtgaacgcga gccatccgag catggaacgg
       61 ttcggcaccg aggaagccac tgtttcggag ctggtagatt tttttcaaac tgaaaaggct
      121 tgcagccttg agcccggcgg caagccttgc gatcactgtg cgatgtgcag ttcgaggggg
      181 ttctagactc ttctatcttc gcagcgagtt ggcgttgatt gtgggcggct gctgcgctcc
      241 ttcctccgct ctgaagtacg gctcctcttt gaatccgaaa agcttcgctg agatcaccgt
      301 cggaaaacgc gcgcgcgtcg tgttgtagtc ctgcgtcgcg tcgttgtaat ccttgcgcgc
      361 cgtggcgatg cgattttcag ttccagctac ctcgtcttgt aacttcaaaa agttctggtt
      421 tgactgcaac tgcggatacg cctcattcaa cacgagaaga cgtccgatgg tgccgccgaa
      481 agtattcgcg gcatcgatca ccgcctgttt ttgttccggc gacttatcgc caccctcgcc
      541 ctgcggcgct tgctgtgtcg cattaagtaa ccgtgaacgc gcctcggcga ttgtgccaaa
      601 gacttcctgc tcctgaatcc cggccagctt tgcggactca accaggttgt ttatgagatc
      661 ggcgcgacgc tgcagttgcg tctcgacgtt ggcccacttt cctttgacgt tctgttgctt
      721 ggttgtcaac gtgttgtagc cgcaaccact ggcgccgagc gaaatcacgg ccaccgcaag
      781 aagaattagc actgttctgg tcttcatatt cgatctcccc ttcgttccaa ttcatcaact
      841 gcttccacca cttgctcgat ctgaaacagg taagctgcaa aaagttcgtt cgcttctttc
      901 tcggatgccg gccacctgcc gccggcacga aactcgaaaa tcgtttcaaa cggcgtgatg
      961 tcgagtttca gcatcttcgt cgttgcgcgc acacattcaa cgttcgtcac cggcgcttcc
     1021 tgtccaaaca ctagcagcgc cgcacgaaat aaaaccgcaa agctcgacaa actgtcgctc
     1081 atcaagtcgc acaacttttc gatcgagatc gacgcgggaa tgtaatgacg ccggagttgg
     1141 attagtttgc tgcgcagttc gtactcagtc tggtgacgca ggtgtgcatc cgacagcttc
     1201 acaaactcaa acggatcgtg accgtagagc acggtgcgtg cggttgccat ctgatgaaac
     1261 tcgataggaa acacatcacc cgcgtccgac agttcctcga ccgtaaaata gaccggcaat
     1321 gggtatccaa gccgttgcca ttcgcgcatc ggcgcctgcg cctgacgcag atcttcgggc
     1381 gtgatgcgat tgagcgcgac gagcaggttg tagtcttgcg gcaactcgtt gcgatcgtct
     1441 gccacagccg gaccataaag gaccacgcac gccagattct ctccgtgcgt cgcacgcaga
     1501 tcatcgacga gtccgttcaa agcttgctgc actaccgctt tcaccatagc aattcgtgtg
     1561 ctccggatac taacaaaacc gctaccagct gccacctgcg ccgccaccgc cgaagctgcc
     1621 gcctccgccg aaacctccgc caaaaccgcc accgccaccg ccaccgccac cgaatcctcc
     1681 accgccgaag cctccgcccc aaccgccgcg cgagcgaccg cttctgccgc cattgctcag
     1741 caggctggcg agtaacgcag cctgccacca tccaccgccc cagccgcctc tatgtccccc
     1801 acgtggacca ccacgcgacc caagcgacac ggcgatcagg atcgcaagaa ggaccaatgc
     1861 gccacagcag atagtgctga actttgttcc tgggatttct ctgatccgga tatcgccttc
     1921 cggttcggca cggtatgcgt aattctgatc gatgccttca acactgaagc cacggttctg
     1981 cgcgagagta gcgacgtagg cctgaaccgt gtcgtaaatg cccttgctgt aatttccctg
     2041 tctgaatgcc ggcaccagtc gttcgcgttg aatctggccc acgaggccgt ccggcagatc
     2101 gccttcgaga tgatcgctga tctgcgtgaa gtacttgcga tcgttgatgg ccaccaccag
     2161 cagaaagccg gccttctcac cttcttttgg tccaatgccc caaccgcgcg cgacagcgag
     2221 agagaagtcg aaaatgtcct gttcgccggt gctcggcacg gttagcaccg cgtactcaat
     2281 gtttccgcgc tctttcaggt ttcgaaagat cgactccaga cgttcacgcg tttgcgcgtc
     2341 gatgacgttt gcgttgtcta cgatgggagt aaacggaacg ggaaggggaa caggtgattg
     2401 aggagtactc tgcgcctggg cgaagccagg cagcaacaaa aagagcaaag ccagcaggcc
     2461 gattccaaga cgctgccgca actccgagtt tttcaagcgc aaatgcaatt actgacttct
     2521 cgccccagtt aaccctctat caccttatgc agcaggtaga agagcgcaac gcctacaaaa
     2581 acgacaattg aaaccgtggg gtttcgttct tctttatggt tcacttcggg gatcaggtcg
     2641 ctcgctgcga cgtagagggt tacgccggcc gagaagggta acgcatatgc aacagcatca
     2701 gtcatacgcg cgtcaagtag agcgactgct atgacgcccg caagcgtcgc cgcgccgatc
     2761 gccgcggtcg cgattaaagc cttctgcgtc gaacgcccgg acgcaagcac gatggaggcg
     2821 acggtgaaac cttcgggcat cttgtgcagc agtatcgcga tgaacacgag catcccaact
     2881 ctgaagttca ccaacgaagc agaggcgatc gacactccgt cgaagaaagt gtgaatgcaa
     2941 agcccgccga cagcggtata cgcggccgag cgtcgcatga acgactcggg gtgcgtttca
     3001 gcgccgaagt gaaaatgggg agcgagagtg tgctcgaaga attgaatcag cagataaccc
     3061 gctaacagca gagtcatcgc tccgacaacg gcttccgaag cggactcgcc gcgcgctccg
     3121 ctggtccaaa ggctgatgct ctccggaagg atctcgataa agatcgcggc gagcatgaat
     3181 ccggcgccga gtgcgacgag atatttgaga aaccgctccc cccagcggtg catacccgac
     3241 ttcactaaaa ccagaccgcc aaggacgttc gcgcccgcag cgagcaagcc aaagaggaga
     3301 atggatgtca tcgtgtgagg ggaatataca ctatggcgaa atccgcgaca ggcatacaag
     3361 aggcgatcat ttcgagccga acacagtatt gacagtg
//
LOCUS       DASTEY010000121         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_233392, whole genome shotgun sequence.
ACCESSION   DASTEY010000121 DASTEY010000000
VERSION     DASTEY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233392"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1026)
                     /locus_tag="VFO72_02085"
     CDS             complement(94..1026)
                     /locus_tag="VFO72_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9478104.1"
                     /translation="MNPTKNRQRWAVILAGGDGTRLQSLTRTISGDDRPKQFCPIIGG
                     RPLLDQTRQRVALSVPAEQTLTIVTETHERFYKPLLKNTLKNRLLVQPANKGTAPAIL
                     LSLLRVAQLSPNATVAFFPSDHFFADDFEFMSHIETAFAALQTGSRMIVLLGIKPEGA
                     EVEYGWIEPEPSLLGGLPRAITRVRRFWEKPPRTLARKLMRQGSLWNSFVMVGHVQAF
                     LNMTARALPELYEAFVEVAPSFGTRLGRQSLNDLYSSLSSSNFSHEVLALRPEDLTVM
                     QVSEVGWSDLGEPKRVFSTLQRIGLQTQWATPVA"
     gene            complement(1103..>3337)
                     /locus_tag="VFO72_02090"
     CDS             complement(1103..>3337)
                     /locus_tag="VFO72_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478105.1"
                     /translation="APEFELIDTGIFNDDRYFDVFVEYAKAGVEDILIKITVINRGPE
                     EASLNLLPTVWFRNTWSWNEKATAKPSLRTTNKAIIELNHAGLGKRWLYCDGEPELLF
                     TENDTNTQRLFNFTNGSHYVKDGINDYVVHGNQQSVNPEQTGTKAAANYELTIAPGAT
                     ATVRLRLTDLSHNNRQEAFASFEKIFNARLHEADEFYETVIPNHLSDDARLVMRQAFA
                     GMLWSKQFYHYVIEQWLDGDPATPKPPAERRNGRNREWKHLYNADVISMPDKWEYPWY
                     AAWDLAFHCVPLALVDSEFAKEQLILMLREWYMHPNGQLPAYEWALGDVNPPVHAWAA
                     WRVYKIEKKRRGVGDTVFLKRVFQKLLLNFTWWVNRKDAEGMNIVQGGFLGLDNIGVF
                     DRNAPLPTGGHIAQSDGTSWMAMYALNLLAIAMELAKEDRSYEDVASKFWEHFLYIAR
                     AMNNRGEEEIKLWDEADGFFYDVLHLPDGKSFPMKVRSMVGLIPLFAVETLEPELLDQ
                     LPDFKRRLEWFINNRADLTENVACMRTPGNAERRLLSIVNRDQLRRVLRFMLDENEFL
                     SPYGIRAVSRYHKDNPYLLQVNGTEHRVDYEPGESSSGLFGGNSNWRGPIWFPVNYLL
                     VESLQKFDHYLGDDFKVECPSGSGKMLTLAEVAAEISRRLARPFMRGADGSRPVYGLI
                     EKFQTDPHWRDLIQFHEYFHGDNGSGIGASHQTGWTGLVAKLMQQSGEHEKKAQAAKS
                     AIHL"
BASE COUNT          728 a          942 c          898 g          769 t
ORIGIN      
        1 atggtttggg catctccgcg agatgcacgg tgttcggctt tccgggaaag actgcaattg
       61 ctttcatgat ttacctttct tagcgctcgt agatcacgcc acgggcgtcg cccactgcgt
      121 ttgaagtcca atccgttgca acgttgaaaa tactcgctta ggctcaccca gatcgctcca
      181 gcccacttcg ctgacttgca tgaccgtaag atcttcgggc cgcaatgcga gaacttcatg
      241 cgaaaagttt gacgaggaaa gtgacgagta cagatcgttc agcgactgcc tccccagacg
      301 ggtaccgaac gacggcgcga cttcgacaaa cgcttcgtac agctcaggca gcgcgcgcgc
      361 tgtcatgtta agaaacgcct gcacgtggcc caccattacg aaactgttcc ataacgatcc
      421 ctgacgcatg agtttgcgcg ccagagttcg cggtggtttt tcccaaaacc ttctcacgcg
      481 cgtaatcgcg cgaggcaaac ctcccaacaa cgacggctcc ggttcgatcc agccgtattc
      541 cacttcggcg ccctccggct tgataccgag taagacgatc atcctggagc cggtttgcag
      601 cgcggcgaaa gctgtctcga tgtgcgacat gaactcgaag tcatcagcga agaaatgatc
      661 ggacgggaaa aaagcgactg tcgcgttcgg tgaaagctgc gcgacgcgca acaagctaag
      721 gagaatcgcc ggcgctgtgc ctttgttcgc cggttgcacc agcagacgat tctttagcgt
      781 gttctttagc agtggtttgt aaaaacgctc atgtgtttcg gtcacgatgg tcagtgtttg
      841 ttcggcgggc accgacaacg caacgcgttg gcgcgtctgg tccaacaacg ggcgaccacc
      901 gatgatcggg cagaactgct taggtcgatc gtcgccggag attgttcgag tgagcgactg
      961 caatcgcgtg ccgtcgccac ccgccaaaat caccgcccat cgctgtctgt tttttgttgg
     1021 gttcatctcg ttcactccgg atatgagtcg gagaaaacca aaattccttt caaaaaaact
     1081 gaaggaaggt tatgggtgtg cgctatagat gaatcgcgct tttggctgcc tgggctttct
     1141 tctcgtgctc accgctctgt tgcatgagct tggcaaccag tccagtccac cctgtctggt
     1201 gactggcgcc gataccggag ccgttgtcgc catggaagta ctcgtgaaac tggatcaggt
     1261 cgcgccagtg cggatccgtc tgaaactttt cgatgagacc gtagactggc cgcgatccat
     1321 ccgcaccgcg catgaacggc cgcgccaagc gccgcgagat ctcggccgcg acttccgcca
     1381 gcgtcagcat cttcccggaa ccggaaggac actcgacctt gaaatcatcg ccgaggtagt
     1441 gatcaaactt ttgtagcgac tcgaccagca agtaattcac tggaaaccag attggaccac
     1501 gccagttcga gttgccgcca aaaagtccgc tgctcgactc tccgggctca taatcaaccc
     1561 ggtgctcggt gccgttcact tgtagtagat acgggttgtc cttgtgataa cgggacaccg
     1621 cgcgaatgcc gtaaggcgag aggaactcgt tctcgtccag catgaatcgc agcacgcgtc
     1681 gcagttgatc tcgattcaca atcgacaaga gtcttcgttc agcgttgccc ggcgtgcgca
     1741 tgcacgcgac gttctcggtc aagtcggccc gattattgat gaaccactcc aaacgccgtt
     1801 tgaaatcggg caactggtcc agcaactccg gctccagcgt ttccaccgcg aagagcggaa
     1861 tcaaaccaac catcgaacga actttcatcg gaaacgattt gccgtcgggc agatgaagca
     1921 cgtcataaaa gaagccgtcg gcttcatccc acaacttgat ctcttcttca ccgcgattgt
     1981 tcatcgcgcg cgcgatgtaa agaaaatgct cccagaattt gctggcaacg tcttcgtacg
     2041 atcgatcctc tttggccagc tccattgcaa tcgccaacag attcagtgcg tacatcgcca
     2101 tccagctggt gccgtccgac tgtgcgatgt gtccgcccgt aggaagtgga gcgttgcgat
     2161 cgaagacgcc gatgttgtcg agaccgagaa aaccgccctg gacaatattc attccctcgg
     2221 cgtctttgcg gttgacccac caggtgaagt tcagcagcag cttttggaag acgcgcttca
     2281 gaaaaacggt atcgcccacg ccgcgtcgtt tcttctcgat cttgtagaca cgccaggctg
     2341 cccacgcatg gaccggagga tttacgtcgc caagagccca ttcgtaggcg ggcagttggc
     2401 cgttcggatg catataccat tcgcgcaaca tcaaaatcag ttgctctttc gcaaactctg
     2461 agtcgaccaa cgccagcggc acgcaatgaa acgcgagatc ccacgcggcg taccacgggt
     2521 attcccactt gtccggcatc gaaatcacgt cggcgttgta gagatgtttc cattcgcggt
     2581 tgcggccatt gcgccgctca gccggcggtt tgggtgtggc tggatcgccg tcaagccatt
     2641 gctcgatcac gtagtgatag aactgtttgg accagagcat gccggcgaaa gcctgtcgca
     2701 tcactaaccg tgcgtcgtcg gaaagatgat ttggaataac cgtttcgtag aactcatcag
     2761 cttcgtgcag tcgcgcgttg aagatctttt caaaactcgc gaaagcctcc tgtcggttgt
     2821 tgtgactgag atctgtcaac cgcaaacgaa cggttgcagt cgcgcctggc gcaattgtca
     2881 gttcgtaatt tgccgccgcc ttcgtgcctg tctgctcggg attaaccgac tgctgattgc
     2941 cgtgaacgac gtagtcgttg ataccgtctt tgacgtagtg cgaaccattc gtgaagttga
     3001 agagccgctg agtgttcgtg tcgttttctg taaacagaag ctccggctcg ccgtcgcagt
     3061 acaaccagcg tttgccgaga ccagcatgat tgagctcgat gattgctttg ttcgttgtgc
     3121 gcaggctcgg tttcgctgtg gccttttcgt tccaggacca ggtgtttcga aaccagaccg
     3181 tgggcagcag gtttaacgaa gcttcctcag gaccgcgatt gatgacagtg atcttgatca
     3241 ggatatcctc gacgccggct ttcgcatatt cgacaaacac gtcgaagtag cgatcgtcgt
     3301 tgaaaatgcc ggtatcgatc agttcaaatt caggtgc
//
LOCUS       DASTEY010000122         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_35904, whole genome shotgun sequence.
ACCESSION   DASTEY010000122 DASTEY010000000
VERSION     DASTEY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35904"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="VFO72_02095"
     CDS             complement(<1..1841)
                     /locus_tag="VFO72_02095"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET9478106.1"
                     /translation="MKRIATLFPLVITLFALFCFALSPNTAITLAAGDSPDPTLYSND
                     WRTTGPPGGDVRGMVVDPSNPDRFYFGTLDGQIYTSADAGKRWQLLYNFGKPRLFVDH
                     IIVDPRNPRVLYVGAHRHDVPGGFFKSTDGGQTWRESSQLKNEALHSLAQSESDPDTL
                     IAGTYTGIFRSDDAGESWKQLPTQSTPGLLHVESLAIDPRTTNTIYAGTFHLPYKSDD
                     GGKTWRSIKEGIIDDSDIFAIDIDPRDPNHVIASACSGIYETKNAGGNWRKVQGIPSQ
                     SRRTRAILQHPSVPGLVFAGTTEGFWRSDKGGDPDSWMVTTSRQLEINSIAVHPSRPD
                     MVFIGTNNYGVMVSNDGGKSFVPTNGGFSGRFANSILADRETPNRIYASTINTATGGG
                     FFFVSNDNGESWRPSMRSMPSRLITYAILQDARDANVIYLGTNLGVYRSLDRGVSWSP
                     VWAVEKKKTPVKKGTKRAVAPKRGVALPNESIIEAQEALAAAGYYRVEPDGKLGPATT
                     AALKKFQSDRHLPVTGKLDAITLGALGIGKPSDPEAGEKSDFILPDAVHALVHTVDPA
                     TQEPAILAATATGLYRSMDPSKGWQKLSYGSYDPRTTCISTNPKQPET"
     gene            complement(2083..>2282)
                     /locus_tag="VFO72_02100"
     CDS             complement(2083..>2282)
                     /locus_tag="VFO72_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478107.1"
                     /translation="VNTRGIEAGLLTVVDGGCRADLTVQLWIVPAGSTPPAAEATGAI
                     SPCPECRRGRRPPRRRRGDEE"
BASE COUNT          478 a          637 c          664 g          503 t
ORIGIN      
        1 gtttccggct gcttcggatt agtggaaata cacgtggtgc gcggatcgta acttccgtaa
       61 gagagcttct gccagccttt cgacggatcc atgctgcgat atagtccggt tgccgttgcc
      121 gccagaattg cgggctcctg agtcgccgga tccacggtat ggaccagcgc gtgaacggcg
      181 tcgggcagaa tgaagtctga cttctctccg gcttcagggt cactcggttt tccgataccg
      241 agcgctccca gagtgattgc atccagcttt ccggttaccg gcaaatgtcg atcggactga
      301 aacttcttca gcgcagcggt tgtggctggt ccaagtttgc cgtcgggttc aacccgataa
      361 taacccgccg ctgcgagcgc ttcctgcgcc tcaataattg attcattcgg tagtgctaca
      421 ccacgctttg gcgccacggc gcgcttcgtt ccttttttca cgggtgtttt cttcttctca
      481 actgcccaca ccggcgacca cgaaacacca cgatcgagcg aacgataaac gccgaggttg
      541 gtgccgagat agatcacgtt tgcatcgcgc gcgtcctgca ggatcgcgta agtgatcaaa
      601 cgcgacggca tgctgcgcat tgaaggacgc cagctctcgc cattgtcgtt actaacgaag
      661 aaaaatcctc cgccagtggc cgtgttgata gtcgaagcgt aaatgcggtt tggtgtttcg
      721 cgatcggcca gaatcgaatt cgcgaaccgg ccgctgaagc cgccgttcgt cggcacgaat
      781 gacttgccgc cgtcgttcga gaccatcacg ccgtagttgt tggtgccgat gaaaaccatg
      841 tcgggacgcg atggatgaac agcgatcgag ttgatctcga gttggcgcga agtggtcacc
      901 atccacgagt caggatcgcc gcccttatcg gagcgccaga aaccttcagt ggtgccggcg
      961 aagacgagtc ccggaacgga cgggtgctgc aagatagcgc gcgtacggcg cgactgcgaa
     1021 gggatgccct gcactttacg ccagtttccg cccgcgttct ttgtctcgta gatgccgctg
     1081 caagcagaag caatgacgtg attgggatcg cgcggatcga tgtcaatggc gaagatgtcg
     1141 gaatcgtcga tgattccttc cttgatcgat ctccacgttt ttccaccgtc gtccgactta
     1201 taaggcagat ggaacgtgcc ggcgtaaatc gtgttggtag tgcgcggatc gatggccaga
     1261 gactcgacgt gcagcagtcc cggagtgctt tgcgttggca attgtttcca cgactcgcca
     1321 gcgtcgtccg accgaaagat cccggtgtac gtaccagcaa tcagagtgtc cggatccgat
     1381 tcggactgcg cgagtgaatg gagtgcttca ttcttcaact gcgaactctc gcgccaggtc
     1441 tggccaccat cagtcgactt aaagaacccg cctggaacgt cgtgccggtg cgcgcctacg
     1501 taaagaacgc gcggattacg cgggtcaacg atgatgtggt cgacgaagag tctcggcttg
     1561 ccgaagttat aaagcaactg ccagcgcttg ccggcgtcgg ctgacgtgta aatctgcccg
     1621 tcaagcgtgc cgaaatagaa tcgatccgga ttcgagggat cgaccaccat gccgcggaca
     1681 tctccacccg gagggccggt agtccgccag tcgttcgaat acagtgtggg atcggggcta
     1741 tcgcctgcgg ccagtgtgat ggcggtgttt ggcgaaagag cgaagcaaaa cagtgcaaag
     1801 agagtaatta cgagggggaa cagcgtagca attcttttca tcgttagttc gtccaggggt
     1861 ctcgaccgtc aggagctaat cgctcacctc gcccctagtt tattacagat tcgttacttt
     1921 gtgtcctaaa agctcgggac gccacatagg atgccccagg aataaagagc agttgtaaac
     1981 aaacactaag ccacagattt tcgcgtacgc gtttatccgc gaaaatctgt ggctaaaagc
     2041 aatcgggcga tgccttgtga gcatcgcccg attggaacgg gtttattcct cgtctcctct
     2101 gcgccgtcgc ggcggtcgtc gaccacgcct acattccgga cacggcgaga tagctcccgt
     2161 ggcttcggct gcaggcggcg tcgagccggc cggtacgatc cagagttgaa cggttaggtc
     2221 tgcacggcag ccaccatcca ccaccgtgag aagtccagct tcgattccgc gcgtattgac
     2281 ga
//
LOCUS       DASTEY010000123         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_662001, whole genome shotgun sequence.
ACCESSION   DASTEY010000123 DASTEY010000000
VERSION     DASTEY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662001"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1086
                     /locus_tag="VFO72_02105"
     CDS             226..1086
                     /locus_tag="VFO72_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478108.1"
                     /translation="MLSQIANVRYLIALLVVIAAFAMGACAALQPIDANGPQPNVPDY
                     PIGLADPAARLEEASVAWYQMSQQYGLPSKTEANLNPYTGTLESLPAGAGTIVLPQVG
                     TEAVPAEEQIRESLRRFIVEWRQLIGADPDELSLVERTDEPSGIKVARYEQRPFRYPL
                     RGGFGNLIIRFRPDRQVVSLSSNCIPHAERIQPELAGLTPTITSEQAIDQVRKQSVVI
                     SGVEGRQQTFSIPSSAALAARQLVVYAQRMTDQTSGLQLHLAWEIEVTNGPIKMVYLD
                     AISNQVIAVA"
     gene            1175..1909
                     /locus_tag="VFO72_02110"
     CDS             1175..1909
                     /locus_tag="VFO72_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9478109.1"
                     /translation="MNNFDPTNNVSWQLGHRGRVAVFIDGNNLFHAARFHNIDIDYNK
                     LLRVLLGDGRLLRAFFYTGVDVGAERQQGFLLWMRRNGFRVIQKELKTFYDGTRKANL
                     DVEIAVDMLSLAGRYDTAVLVSGDEDFVYAVNAVAYKGCRVEIAGFRSNTAPKLIDVA
                     DYFIDLGEIADKVRKEISHVPRYDERELHEPLPSHYLHDEMQMDKSDADAFQTAMRVV
                     LDTQIDEEEAAEFHTATEFIRITDEH"
     gene            1921..2298
                     /locus_tag="VFO72_02115"
     CDS             1921..2298
                     /locus_tag="VFO72_02115"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET9478110.1"
                     /translation="MKLFTIQEANALLPSVRTIVGKIQQAHRNVSRYRDDAKAAAEAA
                     EKGGGGIAAGLEYAEALTELTGRIAELEILGVQLKDFERGLVDFPSLRDGRVVLLCWQ
                     MGEGDELEWWHDVDAGFAGRTPL"
     gene            2508..>2949
                     /locus_tag="VFO72_02120"
     CDS             2508..>2949
                     /locus_tag="VFO72_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478111.1"
                     /translation="MKATGDSPTSPPEQKSTLIEFPGISRSSVPQWRKELSEKVREVQ
                     ERRAREAAREAAEAERQRVEAASNPPQLELLPPTETPAMNPLVAAALKRIERAHQSAS
                     VETSQPRSSLATAVAYAPEREENVMNEMVPPTIQLEFEAEPVNAE"
BASE COUNT          702 a          815 c          813 g          619 t
ORIGIN      
        1 tcagtttccg gcaacggtac gctacagtac aaacagttga tgcgcgtcgg aggattagca
       61 cgcagacact gttcgcagcg aatcatctgc tccggtgtaa aaccttgtgg ctccgaaggt
      121 tctactggtc caagcatagg cagacgaatg ttttcgttat ctttaggcaa cagctcctcc
      181 aagtattttg attatacgac gagccatata atccgaggcg agataatgct ttcgcagatc
      241 gcaaacgtca gatacctgat cgcattgctt gtcgtgatcg ccgcattcgc gatgggcgcc
      301 tgcgctgcgt tgcaaccgat agatgcaaat ggaccacagc ccaacgtgcc ggattatccg
      361 attggcctgg ccgatcctgc cgcgcgactc gaagaggctt ctgtcgcctg gtatcagatg
      421 tcgcagcagt acggattgcc gagtaagacc gaggccaatc tgaatcctta tacaggcacg
      481 ctcgagagtt tgcctgccgg cgctggaacg atagtcctgc cgcaagtagg caccgaagca
      541 gtgccggctg aagaacagat cagagagtcg ttgcgcaggt tcatcgttga atggcgccaa
      601 ttgatcgggg ccgatccgga cgaactctct ctggttgaaa gaacagacga gccatcggga
      661 atcaaagtcg ctcgttatga acagagaccg tttcgctatc cattgcgcgg cgggtttggc
      721 aacctgatca ttcgttttcg tccggaccgg caagtggtgt ctctttcgag caactgtatc
      781 cctcacgccg agcgaataca gccggagctg gctggtctaa ctcccacgat cacttccgag
      841 caggcgatcg atcaggttag aaagcaatct gtcgtcatct ctggcgtcga gggccggcaa
      901 cagacgttta gtattccctc cagcgccgcg ttagcagcac gccaactggt cgtgtatgcg
      961 cagcgcatga cggaccagac cagcggtcta cagctacacc tcgcctggga aatagaagta
     1021 accaatggcc ccataaagat ggtctatctg gacgcgatct caaaccaggt tattgcagtg
     1081 gcttgagcgc gctcaatgga tctgttactt gacccgtgaa cagcgaaaca gcattctaag
     1141 gtcagatcaa tgggtaattg tcggagtcgg gtttatgaac aatttcgatc ccacaaacaa
     1201 tgtttcatgg cagttaggtc accggggaag agtcgcggtt tttattgatg gcaacaacct
     1261 ttttcatgcg gcgcggttcc acaacatcga tatcgattac aacaaacttc tgcgagtgtt
     1321 gctcggcgat ggccggcttt tgcgagcgtt cttctacact ggcgtggacg tcggcgccga
     1381 gcgccaacag ggatttctgc tctggatgcg acgcaatggt ttccgcgtga ttcaaaagga
     1441 actgaagacg ttttacgacg gcacgcgaaa agcaaatctc gacgtcgaga ttgccgtcga
     1501 catgctctcg cttgccggtc gttacgacac ggcagtgctc gtgtcgggcg acgaggattt
     1561 tgtttacgcc gtgaatgccg tggcgtacaa aggctgtcgc gtcgagatcg ccggattcag
     1621 atccaacacc gcgccgaagc tgatcgacgt ggctgattat ttcatcgatc tcggcgagat
     1681 tgccgacaag gtgcgcaagg agatctcgca cgtgccgcgt tatgatgaac gcgaactgca
     1741 cgagcctctg ccctcgcact acttgcacga cgagatgcaa atggacaagt ccgacgcgga
     1801 cgcttttcaa accgcgatgc gagtggtatt agacacacag attgacgagg aggaagcagc
     1861 cgagtttcac acggccacgg agttcatcag aattaccgac gaacactagc gtctcaaaag
     1921 atgaaactgt tcaccattca ggaagcgaac gctcttcttc cgagcgtgag gaccattgtc
     1981 ggcaaaattc agcaggctca tcgaaatgtc tcgcgttacc gcgatgacgc gaaagcagcc
     2041 gctgaagccg ccgaaaaagg cggcggtggc atcgctgccg gtcttgaata cgccgaagca
     2101 ttgaccgagt tgaccggccg gatcgccgag ctggagattc tcggcgtgca actcaaggac
     2161 tttgaacgcg gcctcgtcga ttttccctca cttcgcgacg gccgcgtcgt gttgctctgc
     2221 tggcaaatgg gcgaaggcga cgaactcgag tggtggcatg acgtcgacgc cgggttcgcc
     2281 ggccggaccc cgctctaatg gctcgtggga caggcattcg tgcctgtcct ggtctaggac
     2341 agacaagaat gtttatgcta ccaaaggaac gcacaacctc aactcctgca ttattttctc
     2401 gttgaccagt taaagttatt cctgatagga tgcgtcgcga tcgcgcggag tgattgcagg
     2461 ccgacgcctt aagccggacc cctgtcggtt tatccacttg accgaaaatg aaagcaactg
     2521 gcgactcccc aacttcaccc ccggaacaaa aatctacact catcgaattt cccggtattt
     2581 cgcgcagttc cgtgccccag tggcgcaagg aactcagcga aaaagtacgt gaagtgcagg
     2641 agcgtcgcgc gcgcgaagcc gcccgcgaag cggcagaagc tgaacgccaa cgcgtggaag
     2701 cagccagtaa tcctccccaa ttggaactct taccgccaac cgaaacaccg gcgatgaatc
     2761 cactcgtagc cgccgctctg aaaagaatcg aacgcgcgca ccagagcgct tcggttgaga
     2821 ccagtcaacc gagatcttca ctcgcgaccg cagtcgccta tgcgccggaa cgagaagaaa
     2881 acgtcatgaa cgagatggtt cccccgacca ttcaactcga gtttgaagcg gaacccgtga
     2941 acgcagagc
//
LOCUS       DASTEY010000124         5588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_200505, whole genome shotgun sequence.
ACCESSION   DASTEY010000124 DASTEY010000000
VERSION     DASTEY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200505"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VFO72_02125"
     CDS             <1..787
                     /locus_tag="VFO72_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9478112.1"
                     /translation="SIYPLLYSITISLQQETSAGIVWGLGNFKRLASDNFFRTAMVHT
                     LVYAIAALTCEFLLGLGLALLLNQQIRGRGLFRASLLVPMMLPTVVVGVVWRLMLNPN
                     FGAINGTLKQLGVNTESLTWTASPGLAFLAVVAVDVWQWTPFVFLVLLAGLQAIPQEP
                     YEAALIDGSGRWQTFRHVTLPLLKPAILIVLLLRTMDLLRVFDQIFILTEGGPGFATE
                     MISLYIYRTAFRFFDFGYAAAMSFVLLLITNVVSVAYIRLLRQ"
     gene            784..1581
                     /locus_tag="VFO72_02130"
     CDS             784..1581
                     /locus_tag="VFO72_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9478113.1"
                     /translation="MNHGRTRIGQIGLLVLALLAALAPVYWMITISLKSEVDQFAYPP
                     KWFFFTPTLQHYYEAFLARSFGQYLITSAIVAVVSTGCALVIGTLAAYALTRFRLPYN
                     LDRKLSLWILSTRMFPAIVTAVPLFLMMRDLRLLNTRVSLIVVYTAFNLPFVVWMMRG
                     FFAEVPRDLEEAALVDGDSRLGALVRVVLPLVSPGLAATAVFCLIVSWNEFLFALVLT
                     QTDAAMTLPVGIAGRVTQYEIKWGVMSAAGAVAMMPILIFALAMQRY"
     gene            1620..2684
                     /locus_tag="VFO72_02135"
     CDS             1620..2684
                     /locus_tag="VFO72_02135"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478114.1"
                     /translation="MGIRFQNVTKSFGGLSVVDDLSLEIDDGEFVVLLGPSGCGKTTT
                     LRMLAGLESVTSGDIYIGDERINDVPTQQRDLAMVFQSYALYPHMSIAENIAYPLRVR
                     KLGRDERNNRVARVAKLLEIESLLDRKPRQLSGGERQRVALARAIVREPRAYLMDEPL
                     SNLDARLRVQMRGELKRLQHQLGTTTIYVTHDQAEAMTLASRVAVMKKGRLQQFDTPL
                     NIYKRPANRFVAEFVGSPSMNFIDGEIHGGVFVSDPIRIPVERPDSVVTMGIRPEHIH
                     VFTEPQDGATPASIYVTELMGNETFVFLSVGKHQLIARAPADFHAELESTVWLRIESE
                     KAHFFAQEKSTDYTENTKSV"
     gene            complement(2705..3379)
                     /locus_tag="VFO72_02140"
     CDS             complement(2705..3379)
                     /locus_tag="VFO72_02140"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9478115.1"
                     /translation="MALRLGDIAPDFTADTTEGPIRFHEWIGDSWAILFSHPKDFTPV
                     CTTELGYVARCKPDFDKRGVKVIGLSVDPTDSHKRWAEDIKETQGSELNFPVIADPDH
                     KVAELYDMIHPEISDVFTVRSVFVIGPDKKIKLMITYPASTGRNFDEILRVVDSLQLT
                     AKYSVATPVNWKDGDDVIIVPSLSDEAAKEKFPGGWRAPKPYLRITPQPNKQQAANDT
                     AAKSAS"
     gene            3535..4359
                     /locus_tag="VFO72_02145"
     CDS             3535..4359
                     /locus_tag="VFO72_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9478116.1"
                     /translation="MQIALNGATTMQADLAADFQAAEAAGFDYLEIWADKLKTFLHHR
                     TSLELKDLIAKTGVPPLSINSIEHITFCTPLTYDSIKKQCAELSDIAARIECPYLVVV
                     PSPLPDSGATRSEVIAESVRVLRELCDIAARHNVSLAFEFIGQPDCSVPTLDLAHEIV
                     LTADRPNLGLVIDSFHFYAGGSTIEMVEALDPELIYVFHINDAENLPREQLQDSHRLL
                     PGLGILPLRELLAAFRKIGYDKVASVEIFRPEYWERDPFELAKDARIATERVLQTK"
     gene            4356..5351
                     /locus_tag="VFO72_02150"
     CDS             4356..5351
                     /locus_tag="VFO72_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9478117.1"
                     /translation="MTKIRVGVIGAGYIGGVHAGILARDERVQVAAVYDVERECAERL
                     GRAHDARVASGPDEVIERCDVVYITTPNTQHVALALAAIDAGKHVFCEKPLATNISDA
                     EAVFAKAKNSRSVFQVGHNRRFAPVYATLKQLLSERHVPHSAHVKMNRGELLNPVWTG
                     DPSVTGGFLYETPIHMFDMMRFLFGEVESLHAIGSQHEYREIDAFSVLLKFASGFHAT
                     LATAADAGWLFPFERVEVFCHHATFVTREMESLICSTNLEGHFTEQSMHQLPREEKWG
                     YVQEDRAFIDAIVNGTPPPVTALDGLMSVELVNAVYESVRTNTTIAVRPVSLMAG"
BASE COUNT         1159 a         1490 c         1609 g         1330 t
ORIGIN      
        1 ttcaatctac ccgctgctct actcgatcac gatcagtttg caacaggaga cctcagccgg
       61 aatcgtttgg ggtctcggca acttcaagcg acttgcctca gacaatttct tccgtacggc
      121 gatggtccat acactcgttt acgccatcgc ggctttgacg tgcgagttct tactcgggct
      181 tggcttggcg ctgttgctga accagcaaat ccgcgggcgt ggactgtttc gagcgagtct
      241 gctggtgccg atgatgttgc cgactgtcgt cgtcggtgtc gtgtggcgcc tgatgctgaa
      301 tccaaacttc ggcgccatta acgggacgct caaacagctt ggcgtcaaca ctgaaagttt
      361 gacgtggact gcttcgccgg ggctggcgtt cctcgcggtg gtcgcggtcg atgtttggca
      421 atggacgccg tttgtctttc ttgtgctgct ggcagggttg caggcgatcc cacaggagcc
      481 gtacgaagcc gctctgattg acggatccgg ccggtggcag acgtttcgac atgtgacgtt
      541 gccattgctc aagcccgcga ttctgatcgt gttgctgttg cggacgatgg atctgctgcg
      601 cgtgtttgac cagatcttta ttttgacgga aggcgggccg ggatttgcga ccgagatgat
      661 cagtctttac atctatcgca cggcgtttcg atttttcgat tttgggtacg cggcggcgat
      721 gtcgtttgtg ttgctattga taacgaatgt ggtgagtgtt gcgtatattc gcctgctacg
      781 acaatgaacc acggaaggac acggattgga cagataggac tgctggtgct ggcgctcttg
      841 gcggcgctcg ccccggtcta ctggatgatc acgatctcgt tgaagagcga ggtggatcaa
      901 ttcgcgtatc cgccgaagtg gttctttttt acgccgacgc ttcagcatta ctatgaagcg
      961 tttttggccc gttcgtttgg gcaatatttg atcacgagtg cgatcgtggc ggttgtatca
     1021 acaggctgtg cgcttgtgat tggtacactc gcggcatacg cgctgacgcg ttttcgtctt
     1081 ccctataatc tcgatcgcaa actgtcgctg tggattctct cgacgcggat gtttccggcg
     1141 atcgtcaccg cggtgccgct gtttctcatg atgcgcgact tgcggctgct gaacacgaga
     1201 gtttcgctga tcgttgtcta taccgccttc aacttgccgt tcgtggtgtg gatgatgcgc
     1261 gggttctttg cggaagtgcc acgcgacctc gaagaggctg cgctcgtcga tggcgattcg
     1321 cgtttgggcg cgctggtacg cgtggtgttg ccgctggtta gtccaggttt ggcggcgacg
     1381 gctgtgttct gtttgattgt ttcgtggaac gagtttttgt ttgcgttggt gttaacgcag
     1441 acggatgcgg cgatgacgtt gccggtggga attgccggac gcgtgacgca gtatgagatc
     1501 aagtggggag tgatgagcgc ggcgggcgcg gtagcaatga tgccgatatt gatctttgcg
     1561 ctcgcgatgc agcggtattg agtgcgggga ttgtcgttgg gagctgtaaa gggctgaccg
     1621 tggggattag atttcaaaac gtaacgaaaa gcttcggagg tctgtccgta gtcgatgatc
     1681 tttcgctgga gattgacgac ggcgagtttg tcgtgctgct tggtccatcg ggttgcggca
     1741 agaccacgac gctgcgaatg cttgcgggac tcgaatcggt caccagcggc gatatctaca
     1801 tcggtgatga acgtatcaac gacgtaccaa cacaacaacg cgacctcgca atggtgtttc
     1861 agagttatgc gctgtatcca cacatgagta tcgccgagaa catcgcctac cctttgcgcg
     1921 tgcgaaaact cggtagggat gaacgcaaca accgggtagc gagagtcgcg aagttgttgg
     1981 agatcgagtc gctgctcgat cgcaagccac ggcaactgtc gggtggtgag cggcaacgtg
     2041 ttgcactcgc gcgagcgatc gtgcgtgagc ccagggctta cctgatggac gagccgctgt
     2101 cgaatctcga cgcgcgtttg cgcgtgcaaa tgcgcggcga actcaagcgg ctccagcatc
     2161 aactcggaac caccaccatc tacgtgacgc acgatcaggc ggaagcgatg acgttggcgt
     2221 cgcgtgttgc ggtgatgaag aaagggcggc tacagcagtt tgatactccg ctgaatattt
     2281 acaaacgtcc cgccaatcga tttgtcgcgg agtttgtcgg cagcccgagc atgaatttca
     2341 ttgacggaga gatccacggc ggagtttttg tgagcgaccc aatcagaatt ccagttgagc
     2401 ggcctgattc agtagtgacg atgggaatcc ggcccgaaca cattcacgtt ttcactgaac
     2461 cgcaggatgg agcgacgccg gcaagcattt acgtaactga gttgatgggg aatgagactt
     2521 ttgtgttctt gtccgtaggc aagcaccaat tgatcgcgcg tgcgcccgca gactttcacg
     2581 cagagcttga gagcacagtt tggctaagga ttgagagcga gaaggctcac ttttttgcac
     2641 aagagaaatc cacagattac acagagaata caaagtctgt gtaatctgtg gataggttca
     2701 ctgattaaga agcgctcttc gcggccgtgt cgttcgctgc ttgttgcttg ttcggttgcg
     2761 gagtgatgcg caggtatggc ttcggtgcgc gccaaccgcc cgggaacttc tccttcgcag
     2821 cttcgtccga caacgacggc acgatgatca cgtcgtcgcc gtctttccaa ttcaccggtg
     2881 tcgccacact gtacttcgcc gtcaactgaa gtgaatcgac cacgcgcaga atctcatcga
     2941 aattacgccc ggtgctcgcc ggataggtga tcatcaactt gatcttcttg tctgggccga
     3001 taacgaatac cgaacgcacg gtgaacacgt cgctgatctc cggatggatc atgtcgtaga
     3061 gttcggcaac cttgtggtcc gggtcggcga tgacagggaa attcagttcc gacccctggg
     3121 tctctttgat gtcctccgcc cacctcttat gcgagtcagt aggatcgacg ctcagaccga
     3181 tcactttgac accgcgcttg tcgaagtccg gcttacagcg ggccacgtag ccaagctcgg
     3241 tggtgcaaac tggggtgaag tctttcggat gcgagaacag gatcgcccaa ctatcgccaa
     3301 tccattcatg gaagcggatg ggaccttccg tggtatctgc cgtgaaatcg ggtgctatat
     3361 cgccaagtcg tagagccatt tttgtctcct tttgctgtcg aaaaacgcta atcccggcca
     3421 ccaaaccggg acgataaggt tgcagattaa gttgttcagg atgccaccca cgcttttaca
     3481 actaatggta cggcaagtca aacgacccgc gcacccgtgt gctatccttc ggcgatgcaa
     3541 atcgcactta acggggcgac aacaatgcag gctgatctgg ctgcggattt tcaggcagca
     3601 gaggcggccg gcttcgatta cctcgaaatc tgggccgata agctcaagac gtttctgcac
     3661 catcgcacca gcctggagct caaagaccta atcgcgaaaa ccggcgtgcc gcccttgagt
     3721 atcaactcga tcgagcacat cacgttttgc acgccgttga cctacgattc gatcaaaaaa
     3781 caatgcgcgg agttgagcga catcgccgcc cggatcgaat gcccttacct cgttgtagtg
     3841 ccaagcccgc tgccggactc cggcgccaca cgatcggaag ttattgccga atcagttcgc
     3901 gtgttgcgcg aactctgcga catcgccgcg cgtcacaacg tctcgctcgc attcgaattt
     3961 atcggccaac ccgactgtag cgtgcccaca ctcgacctgg cccacgaaat cgtactgacg
     4021 gccgatcgcc cgaacctcgg gctggtgatt gacagtttcc acttttacgc gggcggctca
     4081 actatcgaaa tggtcgaggc gctcgatcca gaattgatct acgtctttca catcaacgac
     4141 gcggaaaatc tgccgcgcga acagttgcaa gacagtcatc gcctgctgcc cggtcttggc
     4201 attctgcctt tgcgcgaact actcgccgcg tttcgcaaga tcggctatga caaagttgcc
     4261 agcgttgaga tcttccggcc ggaatactgg gagagagatc cgttcgagtt ggcgaaagac
     4321 gcgagaattg cgactgaacg cgtgttacaa accaaatgac taaaatcaga gttggtgtta
     4381 tcggcgccgg ttacatcggc ggtgtgcacg ctggaatact tgcacgtgac gagcgcgtgc
     4441 aggttgcggc agtttacgac gttgaacgtg agtgtgcgga acgactcgga cgcgcgcacg
     4501 acgctcgtgt tgcgagtggg cctgacgagg tgatcgagcg ttgcgatgtg gtctacatca
     4561 ccacgccaaa cacgcaacac gtcgcactcg cgctggccgc cattgacgcc ggcaagcacg
     4621 tcttctgcga gaaaccactg gcaaccaaca tttccgacgc cgaagctgtc ttcgctaaag
     4681 caaagaacag ccgctctgtt tttcaagtag gacacaaccg gcgcttcgct ccagtctacg
     4741 caaccctgaa acaactactg tccgaacggc acgtgcctca ttcagcgcac gtaaagatga
     4801 accgcggcga actgctaaac cctgtctgga ccggcgatcc atccgtcacg ggcggctttc
     4861 tttatgaaac gccgattcac atgttcgaca tgatgcgttt tctgtttggc gaggttgaaa
     4921 gtctgcacgc gattggttcg cagcacgagt atcgcgagat tgacgctttt tccgtgctct
     4981 tgaagttcgc cagcggattt catgcaacgc tggccaccgc cgccgacgca ggctggctgt
     5041 ttcctttcga acgcgtagag gtgttctgcc atcacgcaac cttcgtgacg cgtgagatgg
     5101 agagtctgat ttgtagcacc aaccttgaag gccacttcac tgaacaatcg atgcaccagt
     5161 tgccgcgcga ggaaaaatgg ggctacgtac aggaagatcg ggccttcatc gatgcgatcg
     5221 tcaatggcac gccgccaccg gttaccgctc ttgacgggct tatgtcggtt gagttggtaa
     5281 acgctgttta cgagtctgtc aggaccaata caaccattgc ggtccggccc gtatctttaa
     5341 tggcaggtta gtttggtgct aatatccctt tcacccgacc gagaccccct gttgaacctg
     5401 gtccgattag cccgctttat agtttcgggg gcgtttcatg tcgcaaaatg ccttctcaag
     5461 acgagaagag cgtaccgagc catcggctcg gccagttgaa ctccacgcga acctctaccg
     5521 cgagatcttc gatcattcaa aagaagctat cgcgatcatc gatcgcgacg ggttctacct
     5581 tcaacaga
//
LOCUS       DASTEY010000125         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1387215, whole genome shotgun sequence.
ACCESSION   DASTEY010000125 DASTEY010000000
VERSION     DASTEY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387215"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..>2088
                     /locus_tag="VFO72_02155"
     CDS             101..>2088
                     /locus_tag="VFO72_02155"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET9478118.1"
                     /translation="MRAAQRVAAFSERRGWSTLVKLPSDHVFGHSTHDLLEHFRSRSE
                     PAFFASFKTPEQTAAEFKLRWPGTAQRLIEKADRICDGKFDLLGFKNLDFGNPIDWHY
                     EPLSEKRIPLEHWSKLDFLDAALAGDKKITWELNRHQYFMTLGQAYLLMGDERYASVF
                     TAHIDAWMGANPPKLGINWASSLEVAFRSMSWLWAFYFFKSSQSLSPETFKRAWKFLY
                     LSARHLESYLSTYFSPNTHLTGEALGLFYLGTLLPEFREAKRWQELGRQILIEQLPIH
                     VKPDGVYFEQSSYYHRYTTDFYIHFLLLSRANKVALPPAVEETLVLLLDHLMYITRPD
                     GTTPFFGDDDGGRLAMLDTRPANDFRGTLGTGSVLFERGDYKFVAGDAAEELLWLTGV
                     DGLSKFDAIVATEPEKTSVPFFDGGYFVMRDGWSRESNYLLFDCGPHGALNCGHAHAD
                     ALSIDVAANGRTVLVDPGTCTYTGSKDLRDWFRSSQAHNTLTVDDESSSLPGGPFTWK
                     TTAQCTLQKWIGEKRFDFVSGQHDGYMPTTVKREVLFIKGDYWIIRDTVASSGEHRID
                     VRFHFAPGANLDIQSFGNGEWVEEEAFVSHCYGQKEASKALSFSANLKGVAEVVTFLL
                     PQKPGTDWGVKEIEAREGCAFEVHGANTVDLVMFPAGDA"
BASE COUNT          443 a          582 c          585 g          478 t
ORIGIN      
        1 tagcgcagat cagcgttcat ccgcgttcat ctgcggctaa tgatatattc gattagtgaa
       61 tttggagaaa ctgaggagag cgagccttca agaacttcgc gtgcgtgcgg cgcagagggt
      121 ggcggctttc agcgaacggc gtggctggtc aacgcttgtc aaactgccgt ctgaccacgt
      181 gttcgggcat tcgactcacg atctactcga acactttcgt tcccgatccg aacctgcgtt
      241 ctttgccagt ttcaaaaccc ccgaacaaac cgccgctgaa ttcaaattgc gctggcctgg
      301 gaccgcgcag cggctaattg aaaaggctga ccgtatttgc gacgggaagt tcgacctgct
      361 cggcttcaag aacctcgatt tcggaaatcc gatcgattgg cactacgaac cgctgagcga
      421 aaagcgcatc ccactcgagc actggagcaa gctcgacttc ctcgatgccg cactcgccgg
      481 tgacaagaag atcacatggg aactgaaccg gcaccagtat ttcatgacgc tgggccaggc
      541 ttacctgttg atgggcgacg aacgatacgc gagcgttttc actgctcata tcgacgcgtg
      601 gatgggcgcg aacccgccga agctcggcat caactgggcc agcagtctcg aagtcgcttt
      661 ccgatcgatg tcgtggttgt gggcgttcta cttcttcaag tcgtcgcagt cgttatcgcc
      721 cgagacgttc aagcgcgcgt ggaagttttt gtatctcagc gcgcgccatc tcgaatcgta
      781 cttatcgacg tacttcagtc ccaacacgca tctgaccggc gaagcactcg gactctttta
      841 cctcggaacg ttgctgcctg aattcagaga ggcgaagcgt tggcaggaac tcgggcgcca
      901 gattctgatc gaacagctac cgattcacgt caagcccgac ggcgtctatt ttgaacagtc
      961 cagttactac catcgctaca ccacggactt ctacattcac tttctgctgc tttcacgcgc
     1021 caacaaagtc gcgcttccgc ctgcggttga agagacgctt gtcttactgc tcgatcacct
     1081 gatgtacatc actcgtcctg acggcactac gccgttcttt ggtgatgacg atggcgggcg
     1141 cctggcgatg ctcgatacgc gtccggcaaa tgattttcgc ggcacgttgg gaacgggatc
     1201 ggtgttgttc gaacgtggtg attacaagtt tgtcgccggt gacgcggcgg aagagttgtt
     1261 gtggttaacc ggtgtcgacg gtttgagcaa gtttgatgca atcgtcgcaa ccgagccgga
     1321 aaaaacatcc gtgccgttct tcgatggtgg ttacttcgtc atgcgtgacg gctggtcgcg
     1381 cgagtcgaat tacctgctct tcgactgtgg accacacggg gcacttaact gtggccacgc
     1441 acacgccgac gctctgtcga tcgacgtcgc tgcaaatggt cgcaccgtgc tggtcgatcc
     1501 cggcacctgc acctacacag gctccaaaga cctgcgcgac tggtttcgca gctcgcaagc
     1561 tcacaacaca ttgactgttg acgatgaatc gtcgtctctg ccgggcggcc cgttcacctg
     1621 gaagactact gcacagtgca cgcttcagaa atggattggc gagaaacgct tcgacttcgt
     1681 ttccggccag catgacgggt acatgcccac gacagtgaag cgcgaggtac tgtttataaa
     1741 aggcgattac tggatcatcc gcgacaccgt ggcttcgtca ggcgaacacc gcatcgatgt
     1801 tcgctttcac ttcgcgccag gcgcaaatct cgacatacag agtttcggga atggcgaatg
     1861 ggttgaggaa gaagccttcg tttcgcactg ttatggtcaa aaggaggcgt cgaaagccct
     1921 cagcttttcc gcgaatttga agggcgtcgc cgaggttgtc accttcctgc tgccccaaaa
     1981 acccggcact gactgggggg tgaaggagat cgaagcccgg gaaggctgcg catttgaggt
     2041 tcacggggca aatactgtgg atcttgttat gtttccagcc ggtgacgc
//
LOCUS       DASTEY010000126         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_481038, whole genome shotgun sequence.
ACCESSION   DASTEY010000126 DASTEY010000000
VERSION     DASTEY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481038"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFO72_02160"
     CDS             <1..815
                     /locus_tag="VFO72_02160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9478119.1"
                     /translation="TVKLLGRGGMGSVMLGREQRTGRAVAIKTLLPEFAISDKALKRF
                     MREIDVAAALKHKNIVEFIDRGMHNGVVYLITEYVDGADACKLAEANGGCLSYKDGIS
                     IIAQALEALSYAHSQGYIHRDFKDQNILVSGSSPNLVAKLTDFGLAKSFTHSGMSGVT
                     MAGEMAGTLAYMSPEQLRNFRDVKPQSDIYAVGMTAYSLLSGSLALDLTNKSSVNDTI
                     RAIFEQPAVPLRRRAPHLPAPVCEIIDRALAKDPAQRWQTAAAMRNALLHSV"
     gene            924..1805
                     /locus_tag="VFO72_02165"
     CDS             924..1805
                     /locus_tag="VFO72_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9478120.1"
                     /translation="MRSIWKGHIRFLLVAIPVRIYNAIETSEKIQFNQLHRDDFGPIG
                     YDKRCKKCGEIVTNDQITKGYQYEPDRYAIVEPEDIAKIKIKTTKAVDIIGFVDKDEI
                     PTTFYDAPYFAGPDGSVSEKPYALLREVMKQTGKIGVGKVVLRDREDLVAVFPHEDGL
                     VLQKLHYPHELRKMQDVPDLENVQKLDAQKLNKSELKLATTLVQEMATTLSDIDTADA
                     YHNALRSLIDSKIKGREIAEFEVEEVPRLDIMSALKKSLQASNRKPMVKAPAAAAKKK
                     PQITLVKPRAAKKRKVG"
     gene            1831..2640
                     /locus_tag="VFO72_02170"
     CDS             1831..2640
                     /locus_tag="VFO72_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478121.1"
                     /translation="MKDSSTKVISFPSPVPSKADYQRVKSSYTVKEIKQLFGLSEKTI
                     RRWTEQGIIQASPTSQTPDFVYDFQALTQFRRVRELRAQGQSIRQIEAELQGQLNLFR
                     VEAGRLARLLTPFEEALLLHEQGDPKAAEHYVEAINEGDNVAEAYCNLGIINLERGRL
                     AAALDNFTRSLKNDPRHVEAHYNLANLYYDAGDLQLAKLHYEAAAQIEPGFSLVYFNL
                     SLVYHKLGDLNAASATLEKYRQLEPEDEDLEVLNQLLKAMKSPSPFNQKMD"
     gene            2846..3238
                     /locus_tag="VFO72_02175"
     CDS             2846..3238
                     /locus_tag="VFO72_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478122.1"
                     /translation="MTRPKCRVLCVDDHYDTSEMLELLLSKEDYEVETAGTMEEACRL
                     AAEKQFDLYVLDRRLPDATGSELCQRLQEITPGVPCIFYSGDAYELHRREAIAAGAAA
                     YVAKPDIDGLINAVHKLLANSACATTSK"
     gene            3471..3546
                     /locus_tag="VFO72_02180"
     tRNA            3471..3546
                     /locus_tag="VFO72_02180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3504..3506,aa:Lys,seq:ctt)
     gene            complement(3553..>3881)
                     /locus_tag="VFO72_02185"
     CDS             complement(3553..>3881)
                     /locus_tag="VFO72_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9478123.1"
                     /translation="FTNPDTGTRYNDVKKSFSAACREAAITNFTFHDLRHTFGTRLAD
                     AGVDVVKIKELMGHASIVTTMRYIHATDQGKRGAIVVLSEYRQKRRHKFVTDDKRQTL
                     ESAASH"
BASE COUNT          928 a         1065 c         1056 g          832 t
ORIGIN      
        1 acacagtgaa gctgctcggc cgcggcggca tgggttcggt gatgctgggt cgcgaacaac
       61 gcaccggacg cgccgtcgca atcaaaacgc ttttgccgga gtttgcgatc agcgacaagg
      121 cgctcaaacg ttttatgcgc gagatcgacg tggcggccgc gctcaagcac aagaacatcg
      181 tcgagtttat cgatcgcggc atgcacaacg gcgtcgtcta tctcataacc gaatacgttg
      241 acggcgctga tgcctgcaaa cttgccgaag caaacggtgg ctgcctttct tataaggacg
      301 gcatctcgat catcgcgcaa gcactcgagg cactctctta cgcccactcg cagggctaca
      361 ttcatcgcga tttcaaagac cagaacattc tcgtgtctgg cagctcgcca aacctggtcg
      421 cgaagctgac ggacttcgga ctcgccaaaa gcttcactca ttcgggtatg agcggcgtga
      481 cgatggcggg tgagatggct ggtacgctgg cgtacatgtc gcccgaacaa ctgcgaaact
      541 ttcgcgacgt gaaaccgcag tcagacatct acgccgtcgg catgactgcg tacagtcttc
      601 tgtccggttc gctcgcactc gatctcacca ataaaagcag cgtcaacgac acgattcgcg
      661 ccatcttcga gcagccggcc gtgcctctac ggcggcgtgc gccacacctg ccggccccgg
      721 tctgtgaaat catcgaccgg gcgcttgcaa aggatcctgc acagcgctgg caaacagccg
      781 ccgccatgcg aaacgcgctg ttgcactcgg tttgatgtgt gtcatccgtg gcacaaacct
      841 ctttacttga cgtctattcg accgcataat aaggcgccgc gcgggccgaa ggagcccgcg
      901 tttcgcatca ggagacctta attatgagat cgatctggaa gggtcacata cgctttctgc
      961 tggttgccat ccccgtccgt atttacaacg ccatcgaaac cagcgagaag atccagttca
     1021 accagttgca ccgcgacgac tttggtccaa tcggttacga caagcgctgc aagaagtgcg
     1081 gtgagatcgt gaccaacgat cagatcacga agggttacca gtacgaaccc gatcgctatg
     1141 cgattgtcga acctgaagac attgcgaaga tcaagattaa gactaccaag gcagtagata
     1201 tcatcggatt cgtcgataag gatgagatcc ccactacatt ttatgacgcc ccgtattttg
     1261 ccggcccgga cggctccgtt tctgagaagc cgtacgcgtt gctgcgtgag gtgatgaagc
     1321 agacggggaa gatcggagtg ggtaaagtgg tgctgcgcga tcgtgaagat ctcgtcgcgg
     1381 tgttcccgca cgaagacggt ctcgtgttgc agaagcttca ctacccgcac gagttgcgca
     1441 agatgcagga cgttcccgat ctcgagaacg tgcaaaagct cgatgcgcaa aaactgaaca
     1501 agagtgaatt gaaactcgcc acgacgctcg tgcaggaaat ggccacgacg ctttcagata
     1561 tcgacacggc tgacgcttat cacaatgcgc ttcgcagtct gatcgattca aagatcaaag
     1621 gacgcgagat cgcggaattt gaagttgaag aagtaccaag acttgatatt atgagcgcgc
     1681 tgaagaagag tttgcaggcg tcgaaccgca agcctatggt caaggcgccg gcggccgcag
     1741 cgaagaagaa gccgcagatt acgctcgtga aaccgcgcgc ggccaagaag cgaaaagtcg
     1801 gatagaacta tcactacatt ttcgttgctg atgaaagatt cgagcacgaa ggtaatctcg
     1861 tttccctcac ctgtcccgtc gaaggcagat tatcaacgtg tcaaatcttc ttacaccgtc
     1921 aaagagatca aacagctgtt cggtttgagc gagaagacga ttcgccgctg gaccgaacaa
     1981 ggaatcattc aggcgtcgcc aacgtcgcaa acgcctgact tcgtttacga cttccaggcg
     2041 ttgacgcagt tcaggcgagt gcgcgagttg cgcgcgcagg gtcagagcat tcgccagatc
     2101 gaagccgaac ttcagggaca gttgaatctt ttccgcgtcg aggcagggcg tctggcgcgt
     2161 ttgttgacgc cttttgaaga agcactgcta ctgcacgagc agggcgatcc caaagccgcc
     2221 gaacactacg tcgaagcaat caatgaaggc gacaacgtcg cggaagctta ttgcaacctc
     2281 ggcatcatca atctcgaacg aggccggctc gctgccgcgc tcgacaattt tacgcgttca
     2341 ctcaaaaacg atccgcggca cgtcgaggca cactacaatc tcgcgaacct ttattacgac
     2401 gccggcgatc tccagcttgc aaaattgcac tacgaagcag cggcgcagat cgagccgggc
     2461 ttctcgctcg tttacttcaa cctgtcgctc gtttatcaca aacttggcga cctgaatgcg
     2521 gcctccgcca cgctcgagaa ataccgccag ttagagcctg aagacgagga cttggaagtt
     2581 ctcaatcagc tcttgaaagc catgaagagc ccgtcgccgt ttaaccagaa aatggattga
     2641 tggcggtggg caggagggct gcccacctgg tctggtactt ggatcgcaag aaaggtgaac
     2701 gatccgaatt acccaggtat ttacttcaaa acctccaatt tccttccgct tccagaatcg
     2761 actataatcc gagacgccag cttgatagtt gcccttagtt tcctggataa cccgagagtc
     2821 tgacccaagg cgaaggagtt tccctgtgac ccgtcccaaa tgtcgcgttc tctgtgtgga
     2881 tgatcattac gatacatccg agatgctcga gctgttgctc tccaaagaag attatgaggt
     2941 agaaacagcg ggcacgatgg aagaggcgtg ccgcctggcg gcggagaagc agtttgatct
     3001 gtatgttttg gacaggcgcc tgccggatgc gacggggtcg gagttgtgcc agagactgca
     3061 ggaaataacg cccggcgtgc cgtgtatctt ctacagtggc gacgcgtatg agcttcaccg
     3121 tcgcgaagcg atcgcagcgg gcgcggcagc atacgtagcg aaacccgaca tcgatggttt
     3181 gatcaatgcc gttcacaagt tgctggcgaa tagcgcgtgc gcaaccacca gcaagtgatt
     3241 accgttaggc actcagtagt gatgcagttt gaaaccaggg aacttgtcta cctgggaccg
     3301 caggcgtctc gcctgcaagg tgacggattg tgcgctaacg tgagatgcag gcgagaggcc
     3361 tgcgatccca gcagtaaggc tcttcgattt caaactgcat caccatccaa gcacttcact
     3421 tgcttgacac tccactgatc gcacgtataa tttttcgcgt tcacacgaac gggccgatag
     3481 ctcagttggt agagcagctg actcttaatc agcgggtcgc aggttcgagc cctgctcggc
     3541 tcaccattat tttcaatgac ttgcggcaga ctccagggtc tgccgtttgt cgtctgtgac
     3601 aaacttgtga cgtcgctttt gtcgatactc cgatagaacg acgatcgctc cacgcttccc
     3661 ctggtcggtc gcgtggatat aacgcatcgt cgtcacaatt gacgcgtgtc ccatcaactc
     3721 tttgatcttc acgacgtcaa cgccggcatc tgctaatcga gtaccaaacg tgtgacgcag
     3781 gtcgtgaaac gtgaagttgg ttatggctgc ttcgcgacat gctgcggaaa acgatttctt
     3841 gacatcgttg tacctcgttc ccgtatcagg attcgtaaaa a
//
LOCUS       DASTEY010000127         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1041101, whole genome shotgun sequence.
ACCESSION   DASTEY010000127 DASTEY010000000
VERSION     DASTEY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041101"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..456
                     /locus_tag="VFO72_02190"
     CDS             184..456
                     /locus_tag="VFO72_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET9478124.1"
                     /translation="MDLRVFLTTFGIIFLAEMGDKTQLAAMTMSAQTKNPWAVFLGSS
                     LALVAVSALGVVVGGVIGQYVPLEWVKRAAAVAFIVIGVLMLFGRF"
     gene            473..1171
                     /locus_tag="VFO72_02195"
     CDS             473..1171
                     /locus_tag="VFO72_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET9478125.1"
                     /translation="MPQTPHIERHFTSSEVVRDVVIGMADGLTVPFALAAGLTGAIAQ
                     TGIIVTAGLAEIAAGSIAMGLGGYLAAKSDAEHYAKEREREKREVAEIPHEEMREVAE
                     VFQAYGLSEDETWPIVQALKRQPRNWVDFMMRFELGLEKPEPRRALRSALTIAGAYMA
                     GGMIPLAPYIFANTTSAALPVSIVLTLAALLIFGFVKGRFTGTRPVKSALQTAFIGSV
                     AAGAAFGIAHLIGG"
     gene            complement(1336..>2300)
                     /locus_tag="VFO72_02200"
     CDS             complement(1336..>2300)
                     /locus_tag="VFO72_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478126.1"
                     /translation="DKANLMRAMLKVVGIDAILVSIYSGDPNYVRPNWPSPQQFNHCI
                     IAVKVSDQTQASTIIEHAKLGRLLIFDPTDSETPIGDLPYTLQGSLALVDSKISTELV
                     TMPSTPPEMNQLERFATLQLHADGAIAGQIQENAKGQIAARFRTEFKQLSKPDYHGMI
                     ERWLTAGATSARLNKIEPSDNAGDGQFTLNVEFSANAYGQLMQDRLLVFKPAVVSRRE
                     SLSLTAAQRKHPVVLRANAYSEVVKVQLPPGFDVDEVPDPVKLETAFGTYTTSYEVVG
                     SELVFKRQLSQRATTIPAAQYDVVRKFFENIRAAENAPVVLARK"
BASE COUNT          465 a          635 c          667 g          533 t
ORIGIN      
        1 caacggaaaa ccggtcggtg gctacgagga ttttctggtc ggctggatgc tggggccgga
       61 cgtgaaagaa gtttggggcc gtccggtggg actgctgttt ctggctgatg gttcaatgct
      121 gatcacggac gatggcgcaa acaagatctg gcgcgtcacc tatcgcgcca agtaggagct
      181 ggcatggatc ttcgcgtgtt tctcacgacc tttggcatca tctttctcgc cgaaatgggc
      241 gataaaactc aactggctgc gatgacgatg tcggcgcaga cgaaaaaccc gtgggccgtg
      301 tttctcggat cgagtctcgc gctcgtggcc gtatctgcgc ttggagttgt agtcggcggc
      361 gtgattggac aatatgttcc gctcgaatgg gtgaaacgtg cggcggctgt ggcgtttatt
      421 gtgattggcg tgcttatgtt gttcggcagg ttttgaccac agataaacac ggatgcccca
      481 gactccacac atcgaacgtc acttcacttc gagtgaagtt gtccgcgacg ttgtgattgg
      541 catggctgat ggtctgactg taccgtttgc actcgccgcg ggcctcacgg gcgctatcgc
      601 gcaaacggga atcatcgtca cggcaggact ggctgagatc gctgccggat ctatcgcgat
      661 gggactcggc gggtacctcg cggccaagag cgatgccgaa cactacgcca aagaacgcga
      721 gcgtgaaaaa cgagaggtcg cggaaatccc gcacgaagag atgcgtgaag tcgcggaagt
      781 gtttcaggcc tatggattga gtgaagacga gacgtggccc atcgtgcagg cgttgaaacg
      841 ccagccgcgt aattgggtcg acttcatgat gcgtttcgaa ctcggactcg agaagcctga
      901 gccccgtcgt gcattgagga gcgcgctcac gatcgcggga gcttacatgg ccggcgggat
      961 gattccgctt gcgccataca tctttgctaa cacgacttca gcggcgttac cggtttcaat
     1021 tgtattgacg ctcgccgcgt tgctgatatt tggctttgta aaaggaagat ttaccggcac
     1081 gcggccggtg aaaagcgctc tacaaaccgc cttcatcggc agcgtcgccg ccggcgccgc
     1141 cttcggtatc gcccatctaa tcggcggcta ggaacttata agccgtcttc tttgggaccg
     1201 caggcgtctc gcccgcaatc agccgataga gcttctgctc ctcgtcacag gcaggcgaga
     1261 cgcctgcaat cagcggatag agctctctcg tcacaggcag gcgagacgcc tgcggtccca
     1321 gggaagagcg cgtccttatt tccgcgctaa cacgactggc gcattttcgg cggcgcggat
     1381 gttttcgaaa aacttcctta caacgtcgta ttgcgctgcc ggaatcgtcg tcgctcgttg
     1441 tgacaactgc cgcttgaaca ccagctcact gccgacaact tcataactcg tcgtgtaagt
     1501 accaaaagcc gtctcgagct ttactggatc cgggacttcg tcaacgtcga aaccaggcgg
     1561 cagttgaact ttcacaactt ccgagtacgc attcgcacgc agcacaaccg gatgcttccg
     1621 ttgtgccgca gtaagagaca aactctcgcg gcgcgatacg accgccggtt tgaacaccaa
     1681 caaccggtcc tgcatcaatt gcccataagc gttggcgctg aactcgacgt tcaacgtaaa
     1741 ctgcccatca ccggcgttgt cacttggttc gatcttattt agacgcgccg acgtggctcc
     1801 cgccgtcagc cagcgctcga tcattccgtg gtaatccggt ttggaaagct gcttaaactc
     1861 agtgcgaaac ctcgccgcta tttggccctt tgcgttttcc tgaatctggc cggcgattgc
     1921 gccgtccgcg tgcagttgca atgtcgcgaa gcgttcgagc tgattcatct ctggcggcgt
     1981 gctcggcatg gtcaccaact ctgttgagat ctttgaatcg acgagtgcaa gactgccttg
     2041 cagcgtgtaa ggcaaatcgc cgatcggcgt ttcactgtct gttggatcga agattagcaa
     2101 ccgtccgagt ttcgcgtgct cgatgatcgt gctggcctgc gtctggtcgc tgactttcac
     2161 ggcgataatg cagtgattga actgctgcgg cgacggccag tttgggcgaa cgtagtttgg
     2221 atcccctgaa taaatgctca ccagaatcgc gtcaatgccc acaactttga gcatcgcgcg
     2281 catcaggttt gctttgtctt
//
LOCUS       DASTEY010000128         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1552033, whole genome shotgun sequence.
ACCESSION   DASTEY010000128 DASTEY010000000
VERSION     DASTEY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552033"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..1444
                     /locus_tag="VFO72_02205"
     CDS             377..1444
                     /locus_tag="VFO72_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478127.1"
                     /translation="MSVYGQDPRPLTNKEVVSLLYQLQRSPDMRDQIIEEIRKRGIGF
                     PLTDGMRSLVATKSGNDSLLRRTLEEAERRRVNPAASALPTETEAKELLERTRNVTLA
                     AAGAMPDFLVKQIIKRSVAYGTTANWITQDNLTIAVGYRANAGEEYRVLTVNGLPVGE
                     DVKASSDYSKYAPKGASSSGVEYISALADIFKPESKAEFKMVDTDLIQNRRTVIYEFV
                     VKREFSQLTLTVGDTGARAVVGSRGRMWIDRELDRVLRFEQIATEVPSGFPITAASST
                     IDYDWVSINERKYLLPTHSQILMTTNQGKFMLQSRNDVRFRNYQKFGAELKVVDEVGE
                     DDEPVEETPPKPPKPIKPPQD"
     gene            1670..2074
                     /locus_tag="VFO72_02210"
     CDS             1670..2074
                     /locus_tag="VFO72_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478128.1"
                     /translation="MKPRILIVDDDSVITQQLYWTLCDEYDVVTANDMQTAIRRATFY
                     KPDVSILDLQMPPERNSPASGMRLLEFIKGHLTQSKVLIMSSNSESDVREACIAAGAD
                     GFFAKPFKIEDLLASVCRLIPVHRLKLFAHIL"
     gene            2243..2932
                     /locus_tag="VFO72_02215"
     CDS             2243..2932
                     /locus_tag="VFO72_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9478129.1"
                     /translation="MDKVRGVSELPVFPLPVVLFPGVPLPLHIFEPRYRQMLVDVRST
                     NNLFGVSYFDPTTSTEMAPPAGHVGCVAEVSELQNLADGRSNILTIGVVRYRIDAYVE
                     RGAPYLVAKLSYFEDRDEEDAALAGLTKEVADAFTRIAQAVRTINDERTNLPDISNTE
                     PQRLSFLVAAAMEIDAEIKQELLELRSTFERLERLRAMLSQAVKGYEERARIHELAKG
                     NGHGGKRIEIE"
BASE COUNT          699 a          794 c          816 g          678 t
ORIGIN      
        1 gaaggcgctc aaatgcaggc gcttggcagc gaaccggtgg tgtttggacc cggcgacatt
       61 cgcgaagcac atcgcaccgg tgagtatgtg ccattgcgcg agttggagca gtgcaccaag
      121 attttgcagc acgcggtgga gcaattgtgc gcctgagctc tttccgttaa gctggccttg
      181 cacgaaaagt ctgatttctc tgtgcctttg tgtctcggtg gttgttttcg agtgaaatcg
      241 ttaaccacaa aggcacagag acacaaagac tgcagaagga agttgtcgga tggtactttt
      301 cgtacaaagc ccgttaagct tttcgacgat gtatttcaag aagttaattt gcttgtctgt
      361 tattttgctt tcatccgtgt ccgtttacgg acaggacccg aggcctctca cgaataaaga
      421 agtcgtcagt cttctctatc agttacagcg cagtccggat atgcgcgatc agatcattga
      481 ggagattcgc aaacgcggca tcggttttcc gttgactgac ggcatgcgat cgctggttgc
      541 gacaaaaagc ggcaacgatt ccctgctgcg ccgcacgttg gaagaagcgg aacgacggcg
      601 cgtgaatccc gcggcttcag ctctgccaac agaaaccgaa gcaaaggaac ttctcgaacg
      661 cacgcgaaac gtaacgctcg cagccgcggg tgcgatgcct gactttctcg tcaaacagat
      721 catcaaacgc tcggtcgctt acggcacaac cgccaactgg atcacacagg acaacctgac
      781 gatcgccgtc ggttatcgcg ccaatgccgg cgaggagtat cgcgttctta cagtcaatgg
      841 gctgccggtc ggcgaagacg taaaggcgag ttctgactac agcaagtacg caccgaaagg
      901 cgcgtcgtcg agcggtgtgg agtacatttc agcgctggca gatattttca agcccgagtc
      961 aaaagccgag ttcaagatgg tcgatacgga cttgattcag aaccgtcgca cggtgattta
     1021 cgagttcgtg gtcaagcgag agttttcgca gttgactctg acagtcggcg acaccggcgc
     1081 tcgcgctgtc gtcggttctc gtggacgcat gtggatcgat cgcgaactcg atcgagtgct
     1141 ccgcttcgaa cagattgcaa ctgaagttcc gtctggtttt ccgatcaccg ccgccagcag
     1201 cacgatcgac tacgactggg tctcgatcaa cgaacgcaag tatttgctac cgacgcattc
     1261 gcaaatcctc atgacgacga accagggcaa gtttatgctg caaagtcgga acgatgttcg
     1321 tttccgcaat tatcagaagt ttggcgcgga gctgaaggtc gttgacgaag tcggcgaaga
     1381 cgacgagccg gtcgaggaaa ctccaccgaa accgcccaag ccaatcaaac caccgcagga
     1441 ctaaatactt ctacgcctcg accataaagc cacccacaga ttacgccgat tctgtgtaat
     1501 ctgtggatgg ctcttacagc aaatagtgtt tccgaatgta gacgcctgtt tgccgcttct
     1561 gtgccctcct gtaaacactt gaaccatcac ttagtcgaaa tacgcgtgga tctctgacgg
     1621 tacgagcctt gctgagtaga aggcgaagac agtaggagac ggtttttgaa tgaagcccag
     1681 aatcttaatt gttgatgatg atagtgtcat cacgcaacaa ctctattgga ccttgtgtga
     1741 cgagtacgac gtagtgaccg cgaacgacat gcagacggcc attcgtcgtg cgactttcta
     1801 caagccggat gtttcgatac ttgatttgca gatgccgccg gaacggaact cccccgcaag
     1861 cgggatgcgc ctgttggaat tcatcaaagg ccatctgact cagtcgaagg tcttgatcat
     1921 gagttcaaac agcgagtctg acgtacgtga agcttgcatc gccgccggcg cggatggttt
     1981 cttcgcgaag ccgttcaaga tcgaagacct gctcgccagc gtctgcagat tgataccggt
     2041 tcacaggctt aaattgtttg ctcacattct ttgaagatcc ggacgccacc cactcaaggc
     2101 gtccgtcaaa caccggagat gaaacccctc ttcatcggaa ggtgtgctcg aaggcccccg
     2161 cagcgtctcc cacacgtcgc gggggctgca tttttttaac gcctcctaat ctgccataat
     2221 cagctcacta atgagcgaag ctatggacaa agtacgcggc gtcagcgagt tgcctgtgtt
     2281 tcctttgcct gtggtcctgt ttccaggcgt gcctttgccg ctgcatatct ttgaaccacg
     2341 ctaccggcag atgctcgtcg acgttcgttc cacaaacaat cttttcggcg tctcgtattt
     2401 cgatccgacc acttcgacag aaatggcccc acctgccggt cacgtaggct gcgttgccga
     2461 agtcagcgag ttgcaaaatc tcgccgatgg ccgctcgaac atactgacta ttggtgtggt
     2521 gagataccgg attgacgctt acgtcgaacg aggcgcgccg tatctcgttg cgaagctcag
     2581 ttattttgaa gaccgcgatg aagaggacgc cgcgcttgcg ggcttgacga aggaagtcgc
     2641 cgacgctttt acgcgcattg cgcaagccgt gcgcacgatc aacgacgaac gaacgaactt
     2701 gcccgacatt tccaataccg agccgcaacg tctttcgttt ctcgtcgcgg ccgcgatgga
     2761 gatcgatgcc gagattaaac aggaactgct ggagttgcgc tcgacctttg aacggttgga
     2821 aagactgcga gcgatgcttt cgcaggcggt gaaaggatac gaagagcgag cgcggattca
     2881 cgaactggcg aaaggcaacg ggcacggcgg gaagaggatc gagatcgaat gataactatc
     2941 cgcgttcttt tcttcggcgc cgctcgtgac gtggttgatc aaaatcc
//
LOCUS       DASTEY010000129         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_481110, whole genome shotgun sequence.
ACCESSION   DASTEY010000129 DASTEY010000000
VERSION     DASTEY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481110"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="VFO72_02220"
     CDS             <1..1898
                     /locus_tag="VFO72_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478130.1"
                     /translation="EWFQLEFNGEPWPEAIFDPVRDGDYVVYRRQDGSTVTFCDRQAF
                     LSTDLSNCTIEEPPLFGMIFAPDIWNRIVASAHKYAEPGIAFIDEVNRHNHMMKSMGP
                     IYSCNPCGEQFLHFSNSCNLGSIDLAKFYDPEKRIDWDRLREVTHLCTRFLDNVIDTC
                     AWPLPEINDVVHRTRPVGLGIMGFADLCLNLNITYGSAASIDLMDEVMSFVRRESWME
                     SNRLGAEKGAFPEFEPNREAYTDFLYNQIGIPRDVPLTPRNYEVTTIAPTGTISLVAE
                     TSSGIEPNFSWAYVRKDTLGTRTYVHTLAARALGVDVDQCDQDSIDAAATYVVEHENE
                     LPPHFISAMNISAEQHVHVLAAAQRNVDNSVSKTCNGAVDDTVESVDALYRLGRQLGC
                     KAVSYYRDGSRENQVLNSMKAEEPKGEIACNPEAVVEPLDESVSVAEATAADDASARQ
                     EAIAREHSETVKVPAVTAIRIERPRELRGATWQIPFDGQNLYVTVNHDGKMIQEVFAT
                     GPISGGVGLLASKMLRGGFDAAEVAYSLNKVTGTHAVWFNERLLTSPEQAVAECIMIT
                     VRRVQDLPDSARAVSKQQSQHYGQQKPVTGAIMSNMIGICPECHGQLEHASGCDFCRD
                     CGYSKCK"
     gene            complement(2100..>2368)
                     /locus_tag="VFO72_02225"
     CDS             complement(2100..>2368)
                     /locus_tag="VFO72_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478131.1"
                     /translation="IDINDVDVWKEALESNQWVWAGPGDRGHFRFAGDGVNPNVIVES
                     VRAFQVLWNRNNPEDLIDEDGIFGDIQTGPRLLLSPIEGFPTIS"
BASE COUNT          535 a          648 c          687 g          498 t
ORIGIN      
        1 acgaatggtt tcaactggag ttcaacggtg agccgtggcc tgaagcgatc tttgatcccg
       61 tccgcgatgg tgactacgtg gtctatcgcc gtcaagatgg atcaactgta acgttctgcg
      121 atcgacaggc ttttttgagc acggatcttt caaactgcac gattgaagaa ccaccgctct
      181 tcggcatgat cttcgctccc gacatctgga accggatcgt cgccagtgcg cacaagtacg
      241 ctgaaccggg tatcgctttc atcgacgaag tgaatcgtca caaccacatg atgaagtcga
      301 tgggaccaat ctattcctgc aacccgtgcg gcgaacagtt tctccacttc tcgaattcgt
      361 gcaatctcgg ttcgatcgac ctcgcgaagt tctacgatcc ggagaagcgg atcgattggg
      421 accgtctgcg cgaagtgacg cacctgtgca cgcgttttct cgataacgtg atcgacacct
      481 gtgcctggcc gttgccggag attaacgacg tggtccatcg cacgcgtccg gtgggcctgg
      541 gcatcatggg ttttgcggat ctctgtctga acttgaacat cacttacggt tcggccgcgt
      601 cgatcgatct gatggacgag gtgatgagct ttgtgcgtcg cgagtcgtgg atggaaagca
      661 accggctggg cgcggagaag ggagcgtttc cggagtttga accgaatcgc gaagcgtaca
      721 cggattttct ctacaatcaa atcggcattc cgcgcgacgt tccgttgacg ccgcgcaatt
      781 acgaagtgac gaccattgcg cccaccggga cgatctcgct ggttgccgaa acttcatccg
      841 gtatcgagcc aaacttctcg tgggcatacg ttcgcaaaga cacgctcggc acgcgcactt
      901 acgtgcacac actggcggcg cgcgcgctgg gagttgatgt ggaccaatgc gatcaggatt
      961 cgatagatgc ggctgcgact tatgtcgtgg aacacgaaaa tgagttgccg ccgcacttta
     1021 tctcggcgat gaacataagc gctgagcagc acgttcatgt gctcgcggct gctcagcgca
     1081 acgtcgataa cagtgtgtcg aagacgtgca acggcgcggt tgacgacaca gttgaatcgg
     1141 ttgatgcgtt gtatcgcctc ggacggcagc ttggttgcaa ggccgtcagc tactatcgcg
     1201 atggttcgcg tgagaaccag gtgttgaatt ccatgaaagc ggaagagccg aaaggcgaga
     1261 tcgcttgcaa tccggaagca gtcgtggaac cgttggatga atcggtcagc gtagcggaag
     1321 caacggcagc cgacgatgcg tcggcacgac aggaagccat cgcgcgcgaa cattcagaga
     1381 cggtcaaggt tccggcagtg acggcgatcc gcatcgagcg cccgcgtgaa ctgcgcggcg
     1441 ccacctggca gatcccgttc gacggccaga acctttacgt caccgtcaat cacgacggca
     1501 agatgatcca ggaagtgttt gctactggtc caatcagtgg cggtgtgggc ctgcttgcat
     1561 cgaagatgtt gcgcggcggc tttgacgctg cggaagtggc ttacagtctc aacaaggtga
     1621 ccggaacgca cgccgtctgg ttcaacgaac gattgctgac ttcaccagaa caggcagtcg
     1681 cggagtgcat catgatcact gtgcgacgcg tccaggatct gcccgactcg gcgcgagctg
     1741 tcagtaaaca gcaatcccag cattacggac agcagaagcc ggtaacggga gcaatcatgt
     1801 caaacatgat cggcatctgc cccgagtgtc acggccagct cgagcacgcg agcgggtgtg
     1861 acttctgccg ggattgcggt tacagcaagt gcaagtagtc ttcacttcta ccgggtttgc
     1921 acgaaaagta cccaaatggc gtttgagcac cactctggaa aaaattgtct cgcgcaaagg
     1981 cgcaaaggcg caaaggcgca aagaaaaccc ttaaaaacgc ggtagccgct ttgcgccttt
     2041 gcgcgaggag ttctttcaga aagatgtact tttcgtgcaa agctacttct accttctacc
     2101 taagaaatag tgggaaagcc ttcgatcggc gaaagcaaca ggcgaggacc ggtttggatg
     2161 tcgccgaaga ttccgtcctc gtcgatcaga tcttcgggat tgttgcggtt ccagaggacc
     2221 tgaaacgcgc ggacgctttc aacgatcaca ttcggattga cgccgtcgcc ggcaaacctg
     2281 aagtgaccgc gatcgcccgg cccggcccac acccactggt tgctctcgag cgcctccttc
     2341 cacacatcaa catcattgat gtcgatcg
//
LOCUS       DASTEY010000130         8105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_794124, whole genome shotgun sequence.
ACCESSION   DASTEY010000130 DASTEY010000000
VERSION     DASTEY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794124"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFO72_02230"
     CDS             complement(<1..384)
                     /locus_tag="VFO72_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9478132.1"
                     /translation="MKLLIANGYVIDPAQQVNAGRNLLIENGRVVDLVDRGEAAPEDA
                     QVFDATGLIVAPGFIDLHTHLREPGHEYKETVASGAAAAVAGGWTSICAMPNTDPIND
                     NPAVTRFIIEQAQAAKLANVFPIGAI"
     gene            complement(381..1382)
                     /locus_tag="VFO72_02235"
     CDS             complement(381..1382)
                     /locus_tag="VFO72_02235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9478133.1"
                     /translation="MPTTFNRRHLLGIRDLDAGEITHLLDTAETFRDVSRREIKKVPA
                     LRGRTVINLFFESSTRTRTSFEIAAKRLSADAVNVSVSTSSVTKGETLLDTARNLEAM
                     APDCIVIRHSMAGAPQHLARMCNAPIVNAGDGSHEHPTQALLDALTIREYKRRIEGLR
                     VAIIGDILHSRVARSNIYLLTKLGASVSVSGPGTLVPDEFAGIVDKGLTIEKHFEDAI
                     AGADVVMILRIQRERQDAAFFPSMREYAVHYGLQPRHLERAAPDAIVMHPGPMNRGVE
                     ISSEIADGSRSLILDQVTNGVAVRMAVLYLLAGGSRTQSNGKSNGAPGKKQKRERKA"
     gene            complement(1421..1984)
                     /gene="pyrR"
                     /locus_tag="VFO72_02240"
     CDS             complement(1421..1984)
                     /gene="pyrR"
                     /locus_tag="VFO72_02240"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9478134.1"
                     /translation="MDFIEKARIMDGPRINRALARLSSEVVEENHGAGDLFLVGIRRR
                     GVPLAERMANKIADLEGYRPPVGILDITLYRDDLSTVGAKPVVNRTDLPGDIENRNIV
                     LIDDVLYTGRTIRAALDQLIDFGRPRRVQLAVLIDRGKEHRELPIQADYIGKLVPTKK
                     SEIIKVMLQEFDEEEGVVIVERPSETV"
     gene            2068..3177
                     /locus_tag="VFO72_02245"
     CDS             2068..3177
                     /locus_tag="VFO72_02245"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET9478135.1"
                     /translation="MVYRSLVRRILFSLPPETAHELVLHSLSLLRPKFINNRVAHRYA
                     TSSLPVNRFGISFPNPVGLAAGFDKDGVALPALAALGFGFIEAGTVTFHAQPGNPRPR
                     LFRLPQDKALINRAGFNNAGAAAFAKRVKRQRPQCALGVSIGKSKITPLEKATEDYLA
                     SFELVYDVADYVAVNVSSPNTPQLRELQQAEQLSSLLSALQERNQALQKVRHRERPMP
                     LLVKLSPDLTRDQLEMIVDVLVRLRVEGIIATNTTISREHLLTAQAQVAALGEGGLSG
                     APLRAKSTQIVAQLYRLTRGKIPIVGVGGIFTAEDAWEKIAAGASLVQLYTGFIYRGP
                     GIARDINEGLDRILQQEGFSNLEAAVGCRANERNE"
     gene            3179..3904
                     /gene="pyrF"
                     /locus_tag="VFO72_02250"
     CDS             3179..3904
                     /gene="pyrF"
                     /locus_tag="VFO72_02250"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9478136.1"
                     /translation="MDKLIVALDVDTPAQALSLVKELGPVAGMFKIGSQLFTLAGPQI
                     VKDIVASGAKVFLDLKFHDIPHQVAGAARAAAQLGVSMFTVHASGGAEMMQHAVEAVA
                     EVAEREQTVRSAVLAVSVLTSIDANTLSQIGINSSPEQSVLRLVKLAELSGVDGVVAS
                     PQEAETIRATVANRQFLIVTPGIRPAKKDAGADDQRRVATPAAALKAGADYLVVGRPI
                     TAAADTLAAAREIVAEMEHALVR"
     gene            3964..5496
                     /locus_tag="VFO72_02255"
     CDS             3964..5496
                     /locus_tag="VFO72_02255"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET9478137.1"
                     /translation="MNLSLKPSVIRTLTFLFLALILPVFAQNGGGGAYPFSQAPYQIG
                     ERLTYNVSYSSFPSAAHAELHIVSRGVHHGRDAIELRAHVETTGVVNVALLALNNDYT
                     SYVDPSTGLPFRSEETTRDATRPGDSVHDFNQPAGNEAIPPKQKGSPGTYDFLSAFYR
                     ARALPLAAGGVYSFTVRGEATEHYVELKVTGREAVNTNVGSFNAIVTRVRASNSPLRN
                     LKVYFSDDERHVPVLITAEVGDGDLRAELAASEIVTPVPTVAPTPQIAVRPGPMPTPV
                     VAPTVPVSANEPDWPFTIGEQLNYQIFIGPSTTPMGIATFQVKGRSRYFDRDGLFLTV
                     NAQTTNALARVFTARDQIDSYVEPKALLPFRTVMNLIEGRRRLNQTLTMNQEAGTAQS
                     ESGARIEIPVGTHDYVSFFYAVRTFNLGPNRRSVVSMLVENKPKTLFVNVQKRETIQL
                     GERAIPAIALSITTDDPEPDKYQFRMWISDDKRRLPLRIACATQLGPLRADLAILPAA
                     SQ"
     gene            5726..5953
                     /locus_tag="VFO72_02260"
     CDS             5726..5953
                     /locus_tag="VFO72_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET9478138.1"
                     /translation="MDYRKYITIDPDKRGGKPCVRGLRITVYEVLEYLASEMTEEEIL
                     SDFPDLTHEDLKACIAFAADRERKLMTIPAA"
     gene            6290..7591
                     /locus_tag="VFO72_02265"
     CDS             6290..7591
                     /locus_tag="VFO72_02265"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET9478139.1"
                     /translation="MQLNRREFNYGLALSLGALSLNRFPFQPRFRVNGQRIMEHIFGL
                     AEFGKNPQGGVSRVAYSDADKQGREYVMGLMKAARLDVTIDAGGNIIGRRPGRDNTLK
                     PLLIGSHIDTVPEGGNYDGVVGSLGAIEVAHTLAENGINLLLPLEVVIFQNEEGGLIG
                     SRAMDGELTEKELDLVSRSGKTIREGIKFIGGDPSKLSEAKRKKGDITAYIELHIEQG
                     NTLDTEKINIGIVEGIVGINWWDVTIEGFANHAGTTAMNNRQDALLAAAKFIEAVNRI
                     VTSVPGRQVGTVGRINALPGAPNVIPGKVVLSLELRDLDAGKIKMLYDKIFAEAQQIA
                     ATSRTKFDFKEINVNIPAPTDRRIRGLIGDAAHDLGLTTKLMPSGAGHDAQDMARLGP
                     VGMIFVPSVGGISHSPREFSHPVDIANGANVLLHTVLKLDK"
     gene            complement(7610..>8105)
                     /locus_tag="VFO72_02270"
     CDS             complement(7610..>8105)
                     /locus_tag="VFO72_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478140.1"
                     /translation="AFSAFALYGVNLLESRGLKASIPSVPFAHYVYFALFFLLGYFIY
                     ATIYALVGSMVTTPQEGGQLAMPIILILVVSFYLFLPVSRSPDSSLSFWVSMIPFSAP
                     VAMLVRIVTQTPPFWQIALSLVIGFGTVVLIMWLASRIYRVGMLMYGKRASLPEALRW
                     ARQA"
BASE COUNT         1799 a         2282 c         2317 g         1707 t
ORIGIN      
        1 gattgcaccg atgggaaaga cgttggcgag ttttgccgcc tgtgcttgtt cgatgatgaa
       61 gcgcgtgaca gcgggattat cgttaatggg atcggtgttc ggcatcgcac aaatgctggt
      121 ccacccgccc gcgacagccg ctgcggcgcc cgacgcgaca gtctctttgt attcgtgtcc
      181 cggctcgcgc aggtgcgtgt gtaaatcgat aaagccgggc gcaacgatta agcccgtggc
      241 gtcgaacacc tgagcgtctt cgggagcggc ttcgccgcgg tccacaagat cgacaacacg
      301 gccgttttcg atcaaaagat ttcggccggc gttaacctgc tgcgccggat cgatcacata
      361 tccgttcgcg atcaatagct tcatgccttg cgttctcgct tctgtttttt ccctggagcg
      421 ccgttgcttt ttccattgct ctgggtgcga ctgccacccg cgagcaaata caacactgcc
      481 attcgtactg cgacgccgtt tgtgacctgg tccaaaatca gcgaccggct gccgtctgcg
      541 atttctgagg agatctcgac gccgcggttc atcggtcccg gatgcatgac gatcgcgtcc
      601 ggcgcggctc gttcgagatg gcgcggttgc aagccgtagt gaactgcgta ttcgcgcatt
      661 gacgggaaga acgcagcgtc ctgtcgctcg cgttggatcc gcaggatcat gacaacatcc
      721 gcgccggcga tcgcgtcttc gaaatgtttt tcgatcgtta gtcctttatc gactatgccg
      781 gcgaattcgt ccggaaccag tgtgccggga ccgctgacgc tgacgctcgc gcccagcttt
      841 gtcagcaggt agatatttga gcgtgcgacg cgggagtgga ggatgtcgcc gatgattgcg
      901 acgcgcaaac cctcgatacg tcttttgtat tcgcgaattg tcagcgcatc gagcagcgcc
      961 tgcgttggat gttcgtgtga accgtcgccg gcattgacga tcggcgcgtt gcacatgcgc
     1021 gcgagatgtt gcggcgcgcc cgccatcgag tggcgaatga cgatgcaatc gggcgccatc
     1081 gcttcgaggt tccgagccgt gtcgagtaag gtttcgcctt tcgtgacgct ggaggtcgaa
     1141 acactgacgt tgacggcgtc agccgagagc cgctttgcgg cgatctcaaa ggatgttcga
     1201 gtgcgggtcg aagattcgaa gaatagattg attaccgtgc gtccacgcag ggcgggcact
     1261 tttttgatct cgcggcgcga cacatcgcga aaggtttcgg cggtgtcgag caggtgggtg
     1321 atctcccccg catcgagatc ccgaataccc aacaggtggc ggcgattaaa agttgtcggc
     1381 atctagcaca taaaaaaagc ggccactggc cgcttccccc tcagaccgtc tcagacggcc
     1441 tttccacaat taccacgcct tcttcctcgt cgaattcctg gagcatcact ttgataatct
     1501 cgctcttctt tgtgggcacc agtttcccga tgtaatcagc ctggatcggc aactcgcgat
     1561 gctccttgcc gcgatcgatc agcaccgcta gttgcacgcg acgcgggcgt ccgaagtcga
     1621 tcaactgatc gagcgccgcg cgaatggtgc ggccggtgta aagcacgtcg tcgatcagga
     1681 cgatgttacg attttcgatg tcgccgggaa gatcggtgcg attcacaacg ggctttgcgc
     1741 caacggttga aaggtcgtcg cgatagagcg tgatgtcgag tatgccgacc ggtggccggt
     1801 aaccttcgag gtccgcgatc ttgttggcca tgcgttcggc gagcggcacg ccacggcggc
     1861 gaatcccgac gagaaacaga tcgcccgcac cgtggttttc ctcgactact tcggaagaca
     1921 ggcgcgccaa cgcgcgattg atgcgcgggc catccatgat gcgagccttc tcgatgaaat
     1981 ccatttcggc ggatactatc ataatcgtta acatatgagt aaacagattg acatccgtgt
     2041 catccgtggc tagtctccaa acgattcatg gtctaccgct cactggtgcg ccgcattctt
     2101 ttcagtctcc cgcccgagac cgctcacgaa ttggtgcttc attcgctttc actgctgcgg
     2161 ccaaaattca ttaacaatcg cgtcgcacat cgctatgcaa cgtcttccct gccggtcaat
     2221 cggttcggaa tctctttccc taacccagta ggactggcgg cgggcttcga taaagatggt
     2281 gtcgcccttc ccgccttagc cgcgttgggt tttggcttta tcgaagccgg cacagtgacg
     2341 tttcatgcgc aaccaggaaa tccgcggccg cgtctgtttc gcctgccgca agataaggcg
     2401 cttatcaatc gcgcgggttt caacaatgcg ggcgcggccg cttttgctaa acgcgtgaag
     2461 cgtcagcgtc cgcaatgtgc gctcggcgta agcatcggta agagcaagat aacgcccttg
     2521 gaaaaggcaa ccgaagacta tctggcgagt ttcgagctcg tttatgacgt agcggattac
     2581 gtggccgtaa atgtcagctc gccaaacacc cctcagttgc gtgagcttca gcaagcggag
     2641 caactgtcgt cgctgctgag tgcattgcag gagcgcaatc aagcgttgca gaaggttcgt
     2701 catcgcgagc gaccaatgcc tttgctggtg aaactctcgc cggatctcac gcgcgaccag
     2761 ttggagatga ttgttgacgt ccttgttcgt ttgcgggtgg agggcatcat cgctacgaac
     2821 acaacgatct ctcgcgagca tctgttgacg gcgcaagcgc aagtcgcggc gcttggagaa
     2881 ggaggattga gtggagcacc gttgcgcgcg aaatctacgc agatcgtggc gcagctttac
     2941 cggttaactc gcggcaagat tccgatagtt ggtgtcggtg ggatcttcac tgcggaggac
     3001 gcgtgggaga agattgccgc gggcgcgagt cttgttcagt tgtacactgg cttcatctat
     3061 cgcgggcccg gcatcgctcg cgacattaac gagggtttag atcgcatcct gcagcaggaa
     3121 ggattctcca acctcgaagc ggccgtcggt tgccgcgcaa acgaaaggaa tgaatagttt
     3181 ggacaaactt atagttgcgc ttgacgtaga cacacctgcc caggcgcttt cgctcgtgaa
     3241 agagcttggc ccagttgccg gcatgttcaa gatcggctcg caactgttca cccttgcggg
     3301 accgcagatc gtgaaagata tcgtggcgtc aggcgcaaag gtgtttctcg atctcaagtt
     3361 tcacgacatt ccgcaccagg ttgcaggtgc ggcgcgtgcc gcagcgcagc tcggagtttc
     3421 gatgtttact gtgcacgcga gtggcggcgc cgaaatgatg caacatgcgg ttgaagctgt
     3481 tgccgaagtg gcggagcgcg agcaaacggt ccgttccgcc gtcttggcgg tcagcgttct
     3541 tacgagcatc gatgcaaata cgctttcgca aatcgggatc aactccagtc cggaacaatc
     3601 ggtgttgcgg ctggtgaaac tcgcagaact gtcaggcgtc gacggcgtgg tcgcttcgcc
     3661 acaggaagct gagacgattc gtgcgacagt tgcgaatcgg cagtttttga tagttacacc
     3721 gggaatacgt ccggcgaaaa aagacgcggg tgctgacgat caacgaagag tggcaacacc
     3781 cgccgccgcg ctgaaagcag gcgctgatta tctcgttgtg ggacgcccga taaccgccgc
     3841 tgccgataca ctcgctgccg cgcgtgagat cgttgctgaa atggagcacg cgctggtcag
     3901 ataatggcgg gaactcctga aaagaggtgg cgtgtaaatt gccggaagtt atatcatgcg
     3961 acggtgaatc tcagtttgaa gccctcggtg atccgcactt taacattcct ctttctcgcg
     4021 ctgattcttc cggtttttgc acaaaacgga ggcggcggcg catacccgtt ttcgcaagcg
     4081 ccgtatcaga tcggcgaacg cctcacctat aacgtttcgt actcaagctt tccgagtgcg
     4141 gcgcacgctg agttgcacat cgtctcgcgc ggcgttcacc acggccgcga cgcaatcgaa
     4201 ctccgcgcgc acgtcgaaac cacaggcgtg gtgaacgtcg cgctgctggc actcaacaac
     4261 gactacacga gctacgtcga tccctcaacc ggactgccgt ttcgctctga agaaaccacg
     4321 cgagacgcca cgcggccggg tgactcagtt cacgatttca atcagcccgc cggcaacgaa
     4381 gcgattccgc cgaaacaaaa aggttctccc ggcacctacg atttcctgtc agcgttctat
     4441 cgcgcgcgcg ctctgccgct cgcagccggc ggtgtttaca gttttacagt gcgcggcgaa
     4501 gcgactgaac attatgtcga gttgaaagtc acgggtcgcg aagcagtcaa cacaaacgtc
     4561 gggtctttca acgcgattgt aaccagagta cgcgccagca actcgccact gcgcaaccta
     4621 aaagtgtact tcagcgacga tgaacgtcac gttcccgttc tgataacggc ggaagtaggc
     4681 gacggtgact tgcgtgcgga gctcgctgct tcggagatcg tcacacctgt cccgaccgta
     4741 gctccgacgc cacagatcgc tgttcgacct ggtccaatgc ccacgccggt tgttgctccc
     4801 acagtgccgg tgtccgcaaa cgaaccagac tggccgttca cgattggcga acaactcaac
     4861 tatcagatct ttattggtcc aagcacgacg ccaatgggaa ttgcgacgtt tcaagtgaaa
     4921 gggcgttcac gttatttcga tcgcgacggc ctgttcctga ctgtcaatgc gcaaactacc
     4981 aatgccctgg cgcgcgtctt caccgcgcga gatcagattg attcctacgt cgaacccaaa
     5041 gcgttgttgc cgttccgcac ggtgatgaac ctgatcgaag gccggcgccg gctgaatcag
     5101 acgttaacaa tgaatcagga agccggcacc gcgcaaagcg agtccggcgc gcgcatcgaa
     5161 ataccggtcg gcacacacga ttatgtttct ttcttttacg cggtacggac ttttaatctg
     5221 ggtccgaaca gacgcagcgt ggtatcgatg ctcgtcgaaa acaaaccgaa gacgctcttc
     5281 gtcaacgtgc agaagcggga gacgatccaa ctcggagaac gggcaatacc cgcgatcgcc
     5341 ttgtcaataa ccactgacga tccggagccg gacaagtatc aattccgaat gtggatcagc
     5401 gacgacaaac gccggctgcc gctgcgtatc gcgtgtgcaa ctcaactcgg tcccttgcga
     5461 gctgacctgg caatcctgcc cgctgcctcg cagtagtatt gatgcctttg agcgttttgc
     5521 ttcctgggac cgcaggcgtc tcgcctgccc gggcgcgaag cgggcgcttc cgtgagagtt
     5581 gcaggcgaga cgcccgcggt ccctgcacca gtacaccgct cgtactaaat atggcgccca
     5641 cgtcgagttc acgactacaa aatttggcaa gtaatcaccg agttctctct tgacccggtt
     5701 aagcagcgag acgtaatctg cgaccatgga ctaccgcaag tacatcacaa tcgatcctga
     5761 caaacgcggt ggcaagccgt gtgtgcgagg tttgcgaatc acggtttatg aagtgctgga
     5821 gtatctcgcg tcagagatga ccgaggaaga gatcctcagt gacttccctg acctcactca
     5881 cgaggatcta aaagcgtgca ttgctttcgc agcagatcgc gaacgcaagc tgatgaccat
     5941 tccagccgcg tgaaattgct attcgatgaa aacctctcac atcgtttgat aatccgcatg
     6001 gcggatctcc ttcccgattg tcttcacgtc agagaagttg gtttgcaagc tgcgaatgat
     6061 caggcggttt gggattacgc caagagccat gatttagtta tcgtctcgaa agacggagat
     6121 atgcatcaac gcagttttgt gttcggtgca cctcccaagg tagtgtgggt acgtctcggg
     6181 caattgttca actagtgagg tggaagacct gctgcgacga catgttacac actcaaagca
     6241 ttttacagcg acgactccgc gtcgttcttg actctctcgc gagaaaacaa tgcagcttaa
     6301 tcgccgcgaa ttcaattacg gtctagcgct gagcctgggc gcgttgtcgc ttaatcgctt
     6361 cccttttcaa ccgcgatttc gtgtcaatgg gcagcgcatc atggaacata tttttgggct
     6421 ggcggagttt ggcaagaatc cgcagggtgg cgtgagtcgc gtcgcgtaca gtgatgcgga
     6481 taaacagggc cgcgaatatg tgatggggtt gatgaaagcg gcgcggctcg acgtaacgat
     6541 cgatgccggc ggtaatatca tcggccgcag acctggccgc gacaatacac tcaagccgtt
     6601 gctaatcggc tcgcatatcg acaccgttcc cgaaggcggc aactacgacg gcgtcgtggg
     6661 ctcgttgggc gccatcgagg tcgcgcacac actcgctgaa aacggcataa atctgctgct
     6721 tcccctggaa gtcgtcatct ttcaaaacga agaaggcgga ttgatcggta gccgcgcgat
     6781 ggatggcgag ttgacggaga aagaactcga tctcgtaagc cgcagcggca agacgattcg
     6841 cgaaggtatc aaattcatcg gcggcgaccc gagtaaattg agcgaggcga agcgcaagaa
     6901 gggcgacatc acagcctata tcgagttgca catcgaacaa ggcaacacgc tcgacaccga
     6961 gaagatcaac atcggcatcg tcgaaggcat tgtcgggatc aactggtggg acgtgacgat
     7021 cgaaggtttc gcaaatcacg ccggcacgac ggcgatgaac aatcgccagg atgcgctatt
     7081 ggcggcggca aagtttatcg aagctgtcaa tcgaatcgtg acgagcgtgc ccggcagaca
     7141 agtcggaact gttggccgga tcaatgcttt gccaggggcg ccaaacgtga ttcctggaaa
     7201 ggtcgtgctg agtttagagt tgcgcgatct cgacgcggga aagatcaaga tgctctacga
     7261 caagatcttc gcggaagcac aacagatcgc cgccacgagc agaacgaagt ttgatttcaa
     7321 agagatcaac gtgaacatcc ccgccccgac cgatcgacga atacgcgggt tgatcgggga
     7381 cgccgcgcac gacctcggcc tgacgacaaa actgatgccc tcaggcgcgg gacacgacgc
     7441 gcaggacatg gcgcgacttg gtccagttgg aatgatcttc gtaccaagcg tgggtggcat
     7501 cagtcactcg ccccgcgagt tttcacatcc cgttgacata gccaacggag cgaacgtgct
     7561 gctgcacaca gtgctaaaac tggacaagta aatccgtgta acctgtggct caagcctggc
     7621 gcgcccagcg caaggcctcc ggaagcgaag cgcgcttccc gtacatcagc attcccacgc
     7681 gatagattcg cgacgccagc cacataatca aaactaccgt tccaaaccca attaccaacg
     7741 ataacgcgat ctgccagaac ggcggcgtct gcgtgacgat ccgcaccagc atcgccaccg
     7801 gcgccgaaaa tggaatcatc gagacccaaa acgaaagcga cgaatcagga ctgcgactca
     7861 ccggaagaaa cagataaaaa ctcacgacga gtatcaggat gatcggcatc gccaactgac
     7921 cgccttcctg tggagtcgtg accatcgaac ccacgagcgc gtagatcgtc gcgtagatga
     7981 agtaaccgag cagaaagaac agcgcgaagt aaacgtagtg cgcaaacgga acgctgggaa
     8041 tgctcgcctt cagtcctctc gactcgagca agttcactcc gtacagcgca aaagcgctaa
     8101 acgcc
//
LOCUS       DASTEY010000131         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_381998, whole genome shotgun sequence.
ACCESSION   DASTEY010000131 DASTEY010000000
VERSION     DASTEY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381998"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFO72_02275"
     CDS             complement(<1..398)
                     /locus_tag="VFO72_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478141.1"
                     /translation="MTTAITKVTNHISPNEQLIFERSQTGRIGYRLPALDVDEAELNI
                     PAEFRRDDDLEGMPEVSEVDVIRHFVRMSTWNYSIDLGMYPLGSCTMKYNSRLNERVA
                     RIAGFANLHPLTDEQDAQGALQVIYELQQHL"
     gene            complement(426..1769)
                     /gene="gcvPA"
                     /locus_tag="VFO72_02280"
     CDS             complement(426..1769)
                     /gene="gcvPA"
                     /locus_tag="VFO72_02280"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9478142.1"
                     /translation="MRYIPNSPEERIEMLKAIGLGSAEELFDSIPPDLLLQRPLNTPA
                     ALSEIELLDQFEQMGARNSGARRISFMGGGAYSHYIPTIVDHIISRSEFFTAYTPYQP
                     EISQGTLQTIFEFQTLVCQLTGMEVANASMYDGSTAMAEAVLMAERVTKRSKVIASSA
                     AHPQYLEVVRTYVQHAGIHLELAQFDEQTGQSWQSLAEAIDDSTAALVVQSPNFFGRI
                     EDLAALADAAHAKGALLVVAITEAMSLGLLKSPGACGADIVVAEGQSFGVPLSFGGPY
                     VGLFATREKYARQIPGRLVGEAYDKKGRRGFVLTLATREQHIRREKATSNICTNEGLI
                     ALAATVYLETMGRRGVQEAARQCAQKAAYTARAISGLKGYSLPFSGPFFNEFVVRGPM
                     KAVELLARLGKEKGIDGGIALERFMSDRPNDFLVCVTETNSRAQIDALIEALETI"
     gene            complement(1772..2164)
                     /gene="gcvH"
                     /locus_tag="VFO72_02285"
     CDS             complement(1772..2164)
                     /gene="gcvH"
                     /locus_tag="VFO72_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9478143.1"
                     /translation="MANVPEDLHYSKDHEWVRVDGDEAIIGITDYAQNSLGDVVYVEL
                     PKAGDEFAANEPFGSVESVKAVSEVFTPVAGAITQINESLADEPETVNTDPYGTGWMI
                     RVKMSSPGEVDSLLTAAEYEDFTKAETE"
BASE COUNT          475 a          658 c          586 g          533 t
ORIGIN      
        1 aggtgctgtt gcagttcgta aataacctgt aaagcgccct gtgcgtcctg ctcgtctgtc
       61 agcgggtgaa gattggcgaa gcccgctatc ctggcgacgc gctcgttcaa gcgcgagttg
      121 tacttcatcg tgcacgagcc cagcgggtac atgccgaggt cgatggagta gttccaggtg
      181 gacatgcgga cgaagtgacg gatgacgtcg acttcgctca cttcaggcat gccctcgaga
      241 tcgtcgtcac gacgaaactc cgccgggatg ttcaattctg cttcgtcgac gtcgagagca
      301 ggcaatcgat acccgattcg tccggtttgc gagcgttcga agatgagctg ctcgttcgga
      361 ctgatgtggt tggtgacttt agttatcgcc gttgtcataa ataggtcgta taggacttat
      421 aggtcctata ttgtttcgag cgcttcaatc agcgcatcga tctgcgcgcg gctgtttgtc
      481 tcggtgacgc agaccagaaa atcgtttggc cggtcggaca tgaaccgctc caacgcaatg
      541 ccgccgtcaa ttcctttttc cttacccaga cgcgcaagca actctacggc cttcattggc
      601 ccacgcacca cgaactcatt aaaaaacgga ccgctgaagg gaagtgaata gcccttcaaa
      661 ccggaaatcg cgcgcgccgt gtacgcagct ttttgcgcgc actggcgtgc cgcctcctga
      721 acaccgcgcc ggcccatcgt ttcgaggtaa accgtggctg ccagcgcgat cagtccttca
      781 ttggtgcaga tgttggaagt ggccttctcg cggcggatat gctgctcgcg tgtggccagc
      841 gtcaacacga atccgcgacg gcctttcttg tcgtacgctt caccaacgag ccgtccggga
      901 atctgccgcg cgtacttttc tcgcgtcgca aacaatccca cgtacggacc accaaacgac
      961 aaaggcacac caaacgactg tccttcagcc acgacaatgt ctgcgccaca cgccccgggc
     1021 gacttcaaaa gtcccagcga catcgcttca gtaattgcta caaccagcaa cgcccctttc
     1081 gcatgcgccg catccgcgag cgccgcaaga tcctcaatgc gaccgaagaa attcggcgat
     1141 tggaccacga gggccgctgt cgagtcgtcg attgcttcag ctaacgattg ccacgattgt
     1201 cctgtctgct cgtcaaactg tgccagttca agatgaatcc ccgcgtgctg gacgtaagtt
     1261 ctaacgactt cgagatactg cggatgcgcg gcgctcgaag cgatcacctt cgaacgcttt
     1321 gtgacgcgct cagccatcaa caccgcttcc gccattgcgg tcgacccgtc gtacatcgaa
     1381 gcgttcgcca cttccatccc tgtcaactgg caaaccagcg tttgaaattc gaagatagtt
     1441 tgcagcgtcc cctgcgaaat ctctggttga tacggggtgt aggcagtgaa gaactctgac
     1501 cgcgaaatga tgtgatcgac gatggttgga atgtaatgag agtaagcgcc gcctcccatg
     1561 aaactgatgc gccgcgcgcc gctgttgcgc gcgcccatct gttcaaactg gtcgagcagt
     1621 tctatttcgg atagtgccgc gggagtgttg agcggtcttt gtaaaagcag atccggagga
     1681 attgaatcga acagttcttc ggccgatccg agcccgattg ccttgagcat ttctatgcgc
     1741 tcttccggcg agttaggaat gtagcgcaaa gttattctgt ctcagctttc gtaaaatctt
     1801 catactcagc ggcggtcaac aagctatcga cctcgccggg gctcgacatt ttcacgcgga
     1861 tcatccagcc cgttccgtaa ggatcggtat taactgtttc gggttcatca gccagtgact
     1921 cgttgatttg cgttatcgca ccggcgactg gagtgaagac ttcggaaact gccttcaccg
     1981 actcgaccga gccaaatggt tcattcgctg caaattcatc gcccgctttc ggcagctcga
     2041 catacactac gtcgcccagt gagttttgcg cgtagtcagt gatgccaatg atggcctcat
     2101 caccgtctac ccttacccat tcatgatctt tactgtagtg cagatcttcc ggaacgttcg
     2161 ccatggatcc tccatcttct ttatctattt acttcggtcg tttgtaaaac ggcaccttga
     2221 cgactcgcgc gcctactaac ttgccgcgca cg
//
LOCUS       DASTEY010000132         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1519359, whole genome shotgun sequence.
ACCESSION   DASTEY010000132 DASTEY010000000
VERSION     DASTEY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519359"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFO72_02290"
     CDS             <1..316
                     /locus_tag="VFO72_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478144.1"
                     /translation="DRSVDQINLVVAEINAGRGTLGKLIRDEQIYNDARAAIARFNTT
                     AERIDSLVAAAQRGEGTVGKLLHDETLYTNVNQLSSEGVKMLYDFRQNPKKYLTIKFE
                     LF"
     gene            320..1276
                     /locus_tag="VFO72_02295"
     CDS             320..1276
                     /locus_tag="VFO72_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478145.1"
                     /translation="MRFLSITLLLFVVQTVPAQHSDHRHEPSENVGRVKFTVSCTPQA
                     QKQFNRAVAWLHSFEYEEAEKAFKEVAATDPRCGMAYWGVATSNYHPLWAAPTPAELR
                     RGWEAVERAKLIGTRTQRERDYIAAIELFYKDAETRDHRTRTFAYHEAMKRLYEANRS
                     DAEAGVFYALTLIATGMMSNDKTYVREKEAAQILNRVLARQPQHPGVAHYLIHSYDYP
                     ALAHLALPAARSYAKIAPASAHAQHMPSHIFIRLGLWHEAIRSNLDAEVSAKAFARRN
                     KMPGAWDEQIHAMDYLAYAYLQSGQDKLALGVLEDVKSIKAV"
     gene            1391..1966
                     /locus_tag="VFO72_02300"
     CDS             1391..1966
                     /locus_tag="VFO72_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478146.1"
                     /translation="MFPWQKFRWAEAHIHFARAIGFARSGDTDSARQEVEKLTALRDA
                     LVIGKGEYDWAKQVDIERQVAAAWLAFAEGKGEESVKLMRAAADLDDATDKHPVTPGS
                     VLPAREQLGELLLELKQPSAALAEFELSLRSTPERFNGLYGAARAASLAADQKKARSY
                     YEKLIASTRQADSTRPEINEAKAFLAGVSKN"
     gene            complement(2126..>3004)
                     /locus_tag="VFO72_02305"
     CDS             complement(2126..>3004)
                     /locus_tag="VFO72_02305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9478147.1"
                     /translation="ARYFAKLYDELGRPSDCAIVEGGAGEGRFAQGVLRALSSQYPLL
                     FNATHYYLHDLSEDSRARARQTLHEFPDRVSYGMPETPQTGIYFSNELLDAFPVHRVV
                     NEGELYVTLDHENKFTWSAGPLSKPRLAQFVEEHSIRLAPGQTIEINLAIDDWLTSVA
                     AKLEHGYVITVDYGAESSELYDPSLRRDGTLRAFSRHGFVDNVLSAPGEYDITSSVNW
                     TQVKAAGERLGFEVVEFASQDRFLLSAGLLEELQKGLQQIEKEADKMRLTTGAREMIL
                     PGGMASSFQVLVQKRR"
BASE COUNT          686 a          848 c          849 g          621 t
ORIGIN      
        1 cgaccggtca gtagatcaga tcaacctcgt agttgcggag atcaacgccg gacgcggaac
       61 gctcggcaaa ttgatcagag acgaacagat ctacaacgac gcacgcgcgg cgatcgcgcg
      121 cttcaacact accgccgaac gtatcgacag tcttgtcgct gcggcacaac gcggcgaagg
      181 caccgtcggc aaactgctcc acgatgagac gctctacaca aacgttaacc aattgtcgtc
      241 cgaaggcgtg aagatgcttt acgacttccg gcaaaatccg aagaagtatc tgaccattaa
      301 atttgagttg ttctaagcca tgcgttttct atccatcact ctccttttgt ttgtcgtcca
      361 gacggttccg gcgcaacaca gcgatcaccg ccatgaacct tctgagaatg tcgggcgcgt
      421 gaagttcact gtctcgtgca cgccgcaagc gcaaaagcag ttcaatcgtg ccgtggcctg
      481 gcttcattct ttcgaatacg aagaggccga aaaagccttc aaggaagtag cggccaccga
      541 tccgcgctgc ggcatggcgt actggggcgt tgcgacgagc aactatcatc cgctctgggc
      601 ggcgcccacg ccggccgagt tgaggcgcgg ttgggaggcc gtcgaaaggg cgaagctcat
      661 cggcacgcgc acgcagcgcg aacgtgacta catcgcggcg atcgaactct tttacaaaga
      721 tgcggaaacc cgcgaccatc gcacgaggac gtttgcgtat cacgaggcga tgaaacgcct
      781 ttacgaggcg aatcgatcag acgcggaggc aggcgtcttc tacgcgttga cgctgattgc
      841 gaccgggatg atgtcgaatg acaaaaccta cgtgcgagaa aaggaagccg ctcagatttt
      901 gaatcgcgtg cttgcgcgcc aaccgcagca tcccggcgtc gcgcattacc tgattcacag
      961 ctacgactat cccgcgttgg cccacctggc gctgcctgcg gcgcgcagct acgcgaagat
     1021 tgctccggca tccgcacacg cgcagcacat gccttcgcac atcttcatcc gccttggatt
     1081 gtggcacgaa gctattcgtt ccaatctgga cgctgaagtt tcagcaaagg cgtttgcgcg
     1141 ccggaacaag atgcccggag cctgggacga gcagattcac gcaatggact atcttgctta
     1201 tgcgtacctg caaagcgggc aggacaagtt ggcgttgggt gtgcttgaag atgtgaagag
     1261 cattaaggct gtctagccgc agactttcaa agtcgcctac gctttcgcgg cgattccagc
     1321 gcgttatgtt cttgaaagga agcgctggga cgaagcggcc aaacttacgt tgccggaaaa
     1381 caccgtaggc atgtttccgt ggcaaaagtt ccgctgggct gaagcacaca tccattttgc
     1441 acgtgcgatc ggttttgcgc gtagcggtga cactgactct gcccgccagg aagttgaaaa
     1501 gcttactgcg ctgcgtgatg cgctggttat cggaaagggc gaatacgatt gggccaagca
     1561 agtcgacatc gaacggcagg ttgccgcggc gtggctggcg ttcgcggaag gtaaaggtga
     1621 ggagtcagtg aagctgatgc gcgctgcggc ggatctggac gatgcgaccg acaaacatcc
     1681 ggtgactccc ggttcggttc tgccggcacg cgaacaactc ggcgaacttc tgctcgagtt
     1741 gaagcaaccg tcggcggctc tggcggagtt cgagctctca ctacgcagca caccggaacg
     1801 tttcaacggt ctctacggcg cggcccgcgc ggcgagtttg gcagcggatc agaagaaggc
     1861 gagaagttat tacgagaagt tgatcgcgtc cacgcgccag gctgacagca cgcggcccga
     1921 gatcaatgag gcgaaggcgt ttctcgcggg ggtttcgaag aactgactta acggggtttg
     1981 cacgaaaagt acctatgccg tttgaggacc accgtggaaa aaaatttgtc tcgcgcaaag
     2041 gcgcaaagaa aacccttaaa aacgcgatag cgctttgcgc ctttgcgcgc gtttgatctt
     2101 tgccggtacg gcgaagaact taaaactatc tccgtttctg caccagcact tgaaaactcg
     2161 acgccattcc accgggcaga atcatttccc gcgcaccagt cgtcagccgc atcttatccg
     2221 cctctttttc aatctgctgt aatcccttct gcagttcctc cagcaaaccg gcactcagaa
     2281 gaaaccgatc ctgcgacgca aattcaacga cttcaaaccc caaccgttca ccggccgctt
     2341 tcacctgtgt ccagttcaca ctgctggtga tgtcgtactc gcccggcgcg ctcagcacat
     2401 tatctacaaa tccgtgtcgt gaaaacgcgc gcaacgttcc gtcacgtctt aaagatggat
     2461 cgtagagctc ggacgattca gcaccgtagt caactgtgat cacgtagccg tgctccagtt
     2521 tcgcggccac agacgttaac caatcgtcaa tcgcgaggtt gatctcgatc gtctgccctg
     2581 gcgctagtct aatagagtgc tcctcgacga actgagcgag tcttggtttc gaaagaggtc
     2641 cggcgctcca ggtgaacttg ttttcgtgat cgagagttac gtaaagctca ccttcgttga
     2701 cgacacgatg aaccgggaag gcgtcgagca actcgttgga gaagtagatc ccagtctgcg
     2761 gcgtttccgg cattccgtac gagacacggt ctgggaattc atgcagtgtc tgccgagcgc
     2821 gagctcgcga atcttcactt aagtcgtgga ggtaataatg cgtcgcgttg aagaggagcg
     2881 gatattggct ggataatgca cgcagcacgc cctgcgcaaa acgtccttca ccagcgccgc
     2941 cctcgacaat ggcgcagtcg gaagggcgcc ctagttcgtc gtacagtttc gcaaagtatc
     3001 gcgc
//
LOCUS       DASTEY010000133         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1370877, whole genome shotgun sequence.
ACCESSION   DASTEY010000133 DASTEY010000000
VERSION     DASTEY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370877"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VFO72_02310"
     CDS             <1..700
                     /locus_tag="VFO72_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9478148.1"
                     /translation="LTKMTLVSSRPFHGHTGRTYKLTIGDLSGSLNAFVTKKRFYAIV
                     SLNTKKDEALEEKFLSSFVLPERQADPPKTAATDVNANAANQQNQVQQPETELPATPA
                     GTEGNTDPNTATPNAANNQQQNPGQPNQNQQQNPADPKKRGPIAGGMLNAKAIYLPLP
                     EVPPGEANGVVLVQVLVDEQGGVIDARPVSGPQHLHAAAVNAARLARFNPTMLMGEPV
                     KVSGTLSYNFVRSN"
     gene            736..>2036
                     /locus_tag="VFO72_02315"
     CDS             736..>2036
                     /locus_tag="VFO72_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478149.1"
                     /translation="MIATLCPAWSIPGYLEVRDDHLTVNGVDTLELVKEFNSPLFVFS
                     ENRIRDNISRLQRAAEAVDRPIKFCYASKANSNMAILRVVLDSGIDIEVNSGGELFKA
                     LRVGFRPDQIIFNGTSKSDDEIDDAVRAGIYAINVDSIYEIELVEQSVERLRARGEQV
                     PAARITLRLVPEIGTRSHLGLQTALLTSKFGISSSEVLAAFRRGLERPDLIKVCGIHI
                     HVGSQTPDVEPFAEAFRSMWEHLVTIHRETGHTLEHINLGGGIPVNYLRDRSQADQLP
                     EHERDMLGAELEPTDVLREALRVARESARAADAEHLLDQVTILLEPGRSVIADAGLVL
                     TTVRNIKSRPETGDVWLLTDAGYNIMLSMNNYKWYYHLISASRAGAPYARDYKVAGPL
                     CDSGDVYFDIEREGRLPDYRKLPENVGPGEVLALLNCGAYSL"
BASE COUNT          467 a          571 c          565 g          433 t
ORIGIN      
        1 tctcactaag atgacgctgg tgagcagtcg tccgtttcat ggtcatacgg gcagaactta
       61 taagctaacg atcggcgact tgagtggatc actgaatgcg tttgttacaa agaagcgttt
      121 ctatgcgatc gtttcactca acacgaagaa ggatgaagcg ctcgaagaga agttcctgag
      181 ctcgtttgtg ttgcccgagc gccaggctga cccgcccaaa accgcggcta cagacgtaaa
      241 cgctaatgct gcgaatcagc agaatcaggt gcaacaaccg gagacagaac ttccggcaac
      301 gcccgccgga acagaaggca acaccgatcc caacactgct acgcccaatg ctgcgaacaa
      361 tcaacagcag aatccgggac agccgaatca gaatcagcag cagaatccgg cagacccgaa
      421 aaagcgcggt cctatcgccg gcggcatgtt gaatgcgaaa gccatttact tgccgttgcc
      481 ggaagttcca ccgggtgagg ccaatggcgt tgtgctggta caggtactgg tcgacgaaca
      541 gggaggcgtg atcgatgcac gtccggtttc tggtccacaa cacttgcacg cagcggccgt
      601 gaatgcggcg cgtctggcaa gattcaatcc gacgatgctg atgggtgagc cggtgaaggt
      661 tagtggtacg ctgagttaca atttcgttcg ttcaaactga gccgcggata aacacatcaa
      721 agccgccgat caagaatgat agctacttta tgccccgcat ggtccatacc tggctacctg
      781 gaagtgcgtg acgatcacct gactgtcaat ggtgtcgata cgctcgaact ggtaaaggaa
      841 ttcaactctc cccttttcgt cttttcggaa aatcgcatac gcgacaacat cagccgtttg
      901 caacgcgccg ctgaagcagt ggatcggccg atcaagtttt gctatgcctc aaaagcaaac
      961 tccaacatgg cgatcctgag agtcgtgctc gattccggta tcgacatcga agtgaacagc
     1021 ggtggcgagc tgttcaaagc gttgcgcgtc ggctttcgtc ccgaccagat catctttaac
     1081 ggcaccagca aatcggacga tgagattgac gacgccgtgc gcgccggaat ttacgctatc
     1141 aatgtcgatt cgatttacga gatcgagctg gtggaacaat ccgtcgagcg cctgcgcgcg
     1201 cgcggtgagc aggtgccggc ggctcgcatc acgttgcgtc tggtccctga aatcggcacg
     1261 cgctcacacc tcggcttaca gactgcgctg ctgacgtcga agttcggaat ctcttcgtcg
     1321 gaagtgctgg ccgcatttcg tcgcggtctc gagcggcctg acctgattaa agtttgcggt
     1381 attcacattc acgtcggatc gcaaacgccc gacgttgaac cgtttgccga agcattcagg
     1441 agcatgtggg aacacctggt cacgattcat cgtgaaacag gacacacgct cgaacacatc
     1501 aatctcggtg gcggcattcc cgttaactac ttgcgcgatc gttcgcaggc ggatcaactt
     1561 ccggaacacg agcgcgacat gttaggcgcc gagctcgagc ccacggatgt gctccgtgaa
     1621 gcgctgcgcg tcgcgcgtga atcagctcgc gctgcggacg ccgagcatct gctcgatcag
     1681 gtgacgatct tattggagcc gggtcgtagc gtcatcgccg atgccggttt agtgctcacc
     1741 accgtgcgca acatcaagag tcgtccggaa accggtgacg tgtggttatt gaccgatgcc
     1801 ggttacaaca tcatgctctc gatgaataac tacaagtggt actaccactt gatctctgcc
     1861 tcccgcgccg gcgcgccgta cgcccgggat tacaaagttg ccggcccgct ctgtgactca
     1921 ggcgacgtct atttcgacat cgaacgcgaa ggacgtttgc ctgactatcg caagcttcct
     1981 gagaacgttg gtccaggtga ggtgctcgca ctcctcaact gcggtgctta ttctct
//
LOCUS       DASTEY010000134         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_481139, whole genome shotgun sequence.
ACCESSION   DASTEY010000134 DASTEY010000000
VERSION     DASTEY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481139"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..941
                     /locus_tag="VFO72_02320"
     CDS             72..941
                     /locus_tag="VFO72_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9478150.1"
                     /translation="MKNVISVTLFALFVLSAFVSVPAQETAGAMPVRPALTDPASTTK
                     SAEPLLTEIYRVGVGDVLDVRLLNSPHNKSTLFTVMPGGAIDFPVAGGAIKVAGLTTD
                     EIQVLVAGELKRRAVEENAQVSVGVRQYASHSVMINGLVVHPGTRYLRREAVPLYVVL
                     AESHLRNDAGRVVIMRAGTNGDVLDLSDPKTLNVTVQSGDAITVSNKPQEFYYIGGRI
                     NFPGQKDFQPGITLLQAILAAGGTRQENRVDISRAGTDGRLTTIRYRIKDIKSGRVED
                     PKLQPGDRIEISK"
     gene            1004..1519
                     /locus_tag="VFO72_02325"
     CDS             1004..1519
                     /locus_tag="VFO72_02325"
                     /inference="COORDINATES: protein motif:HMM:NF036688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5518 domain-containing protein"
                     /protein_id="HET9478151.1"
                     /translation="MNNKLKPALIGGVLLGLLSVIPFVNVLNVCCCLWAILGGMLASY
                     LYIKNSPTPASAGDGAILGALAGVIGAAISLILGIPISYAMGPTMRNLMLSMLENVDR
                     QQAEAMRRQFEMTGDSIVPVIINSLIVAALLFVFAVIGGLIGTALFEKRKGGSVPPPP
                     PATGGPGAYPV"
     gene            1583..1879
                     /locus_tag="VFO72_02330"
     CDS             1583..1879
                     /locus_tag="VFO72_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HET9478152.1"
                     /translation="MSQDWTPPPASGTSENIPNYLVPAILSAICCFPLGIISIVFAAQ
                     VNGKVAAGDIQGAMDASKKAKLFSYIFLGLGIVGWVLSFIFGLVGGILGGMANV"
     gene            1904..2299
                     /locus_tag="VFO72_02335"
     CDS             1904..2299
                     /locus_tag="VFO72_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HET9478153.1"
                     /translation="MTRYCTKCGAINDDTAQYCTNCQTPLSPPIGVGYQPMQSVNPGA
                     MTDWKAMGADKKVLAGILAIVVGAFGVHKFVLGYTTEGIIMLLLTVLTCGVAAAVTSI
                     IGIVEGILYLTKSDEDFVRTYIQNKKGWF"
     gene            2390..2794
                     /locus_tag="VFO72_02340"
     CDS             2390..2794
                     /locus_tag="VFO72_02340"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HET9478154.1"
                     /translation="MPTEFMYRRSTVIGIWLLLIAGAVYAFVFEPGRTGFFPSCPFRV
                     LTGLTCPGCGATRALHEMLHGHFEAAFMLNPLLLLALPLLLYALLRYSTIAMRGRLPR
                     RNALPAPYIYAMFFVIVGFWIFRNTPLYPFVS"
     gene            2805..3818
                     /locus_tag="VFO72_02345"
     CDS             2805..3818
                     /locus_tag="VFO72_02345"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET9478155.1"
                     /translation="MRTLILFFLISVLTNAPEAPAQTPATPTRPVHTYSIVARDPVTG
                     ELGVAVQSHWFSVGPIVAWAEAGVGAVATQSFVDPSYGKNGLELMRAGKSAPETLKEL
                     LAKDEGREVRQVAMIDAQGRVDAWTGKNDIQAAGHIVGNNFSVQANLMLNDKVWPAMA
                     RAFENTRGDLAERMLAALDAAEAAGGDIRGRQSAALIVVTGKPTGQAWKDRTFDLRVD
                     DSPQPLKELRRLVRLQRAYNHMNAGDLAVEKKDNEGALREYSAAERLVPDNAEMIYWH
                     AVALVNMGRVEESLPLFRKVFAMDRNWITLTPRLPKSGLLPDDPKLINRIVSAGSTRS
                     NRR"
BASE COUNT          845 a         1096 c         1063 g          894 t
ORIGIN      
        1 gtttgttggt atataatgcg agaccaacat cttaccaagt aaccgaaact ttcgagcttc
       61 tgccccggct tatgaaaaac gtaatttcag tcacgctctt tgctttgttt gttctttcag
      121 cctttgtttc ggtaccagcg caagagactg ccggcgccat gccggtcagg ccggcactca
      181 ccgacccagc cagcactacc aagtccgccg aacccttgtt aacggagatc tatcgcgtgg
      241 gtgttggcga cgttctggac gtccgtctac ttaattcacc tcacaacaaa tcgacactct
      301 tcaccgtcat gccgggcggc gcaatcgact ttccggttgc gggtggcgca atcaaagtcg
      361 cgggactgac aaccgacgaa attcaggtcc ttgttgccgg cgaacttaag agacgcgcgg
      421 ttgaagaaaa cgcgcaagtt tccgtgggcg tgcgccagta tgcgagccac tcggtaatga
      481 tcaacggtct tgtggttcat cccggtactc gatacctgcg acgcgaggcg gtgccgctgt
      541 atgttgtgct cgccgagtcg catttgcgaa acgacgcggg acgcgtggtg atcatgcgcg
      601 ccggaacaaa cggcgacgtg ctcgatttga gcgatccgaa gacgctcaac gtgactgttc
      661 aatcaggaga tgcgatcacg gtttccaaca agccgcagga gttttattac atcggcgggc
      721 ggatcaattt ccccggacag aaggactttc aaccgggcat cacgttgctc caggcgatac
      781 tcgctgcggg cggcacgcgc caggaaaacc gcgtcgacat ctcgcgcgcg ggaactgacg
      841 gacggctcac gaccattcgc tatcgcatca aggacatcaa atccggccgc gtagaagatc
      901 ctaaacttca gccgggcgac cgcattgaga tttccaagta aaccagcgat aaatatcggc
      961 agttttattg acaatcggag ccctgtttgt tagtagaagt gcgatgaata ataaacttaa
     1021 gcctgcgctg atcggcggcg tcctgctggg cttgctttcc gtaattccct tcgtcaacgt
     1081 cctgaacgtt tgttgctgtc tctgggcgat tttgggaggc atgctggcgt cgtatctcta
     1141 catcaagaat tcacccactc cggcgagcgc cggagacgga gctattcttg gcgccctggc
     1201 tggtgtcatc ggcgcagcga tctcgctcat cctcggaata cccatctcct acgcgatggg
     1261 accgacgatg agaaacttga tgttgagcat gctcgaaaac gtggatcgcc agcaggctga
     1321 agcaatgcgg cgccagttcg agatgaccgg tgattcgatc gttcctgtaa tcatcaattc
     1381 cctgatcgtg gcggccctgc tattcgtttt tgcagtcatc ggcggactga tcggaactgc
     1441 gctgtttgag aagcgtaaag gtggttcggt gccgccgcct ccacctgcta ccggaggccc
     1501 aggcgcttac ccggtataag gtaaccaagc gcagaaattc tttatcgcga agcctttcaa
     1561 cactttctcc aggagaaact taatgtctca agactggact cccccgcccg catcgggcac
     1621 atctgagaac atccccaact atctcgttcc agccatactt tcggcgatct gctgtttccc
     1681 gctgggaatc atcagcattg ttttcgctgc acaagtaaac gggaaggtcg ctgccggcga
     1741 cattcaagga gcgatggatg cgtcgaagaa ggctaagctt ttcagttaca tcttcctcgg
     1801 cctcggcatc gttggttggg tgttgtcgtt tatcttcggt ctggtcggcg ggatcttagg
     1861 cggcatggcc aacgtgtaaa ctcagtttgg agacctcaaa gagatgacca gatactgcac
     1921 aaaatgcggc gcaatcaacg atgacacagc gcagtactgt acaaactgcc agacaccgct
     1981 gagcccgcca attggcgtcg gttatcaacc gatgcagtca gttaatcctg gtgcgatgac
     2041 cgattggaaa gcaatgggcg ccgacaaaaa ggtcctggcc ggaatactcg ccatcgtcgt
     2101 cggagctttt ggcgtgcaca agttcgttct cggttacacg actgaaggaa tcatcatgtt
     2161 gctcctgacg gtgctgactt gcggcgtcgc cgcggccgtc acgagtatca tcggcatcgt
     2221 cgaaggcata ctgtatctca caaagtcaga cgaagacttt gtgagaacgt acattcagaa
     2281 caagaagggc tggttttaac aacgcgcgtc gctttggcat attgaagcaa ctcccgaaga
     2341 acgttgcctc actttatatc gacggctctg ttgtagctta ataggaatca tgccgactga
     2401 gttcatgtac cggcgatcga ctgtgatcgg aatttggtta ctgttgattg ccggtgcggt
     2461 ttatgccttt gtgttcgaac ctggacgaac gggctttttc ccgtcgtgtc cgtttcgagt
     2521 gctaacagga ctgacgtgtc cgggctgcgg cgccacgcga gcgcttcacg aaatgctgca
     2581 cggtcacttc gaagctgcat tcatgcttaa tccgctgcta ctgctcgcgt tgcctctttt
     2641 gctttacgca ttgttgcgtt acagcacgat cgcaatgcgc ggccgcctgc cacggcgtaa
     2701 cgctcttccc gccccttata tttacgcaat gttttttgtc atcgtaggtt tttggatctt
     2761 tcgcaacacg cctttgtacc cattcgtttc gtagaggacg aactatgcgc accctgattc
     2821 ttttttttct tatatccgtg ttaactaatg ccccggaggc gcccgctcag accccagcga
     2881 caccaacacg tcccgttcac acttactcca tcgtcgcgcg cgatcctgtt acaggcgaac
     2941 tcggcgtcgc cgtccaatca cactggtttt ctgttggacc aatcgtagcc tgggctgaag
     3001 cgggtgtcgg cgcggttgcc acgcaatcat tcgttgatcc gagttatggt aaaaatggcc
     3061 tcgagctcat gcgcgccggc aaaagcgccc cggaaacgtt gaaagaactg ctcgccaaag
     3121 acgagggacg cgaagtccgc caggttgcaa tgatcgacgc gcagggtcgg gtggacgcat
     3181 ggaccggcaa gaacgacatt caggccgcgg gccatatcgt gggcaataac ttttccgtgc
     3241 aagcgaactt gatgctcaac gacaaagtgt ggcccgcaat ggcacgtgca ttcgaaaaca
     3301 cgcgcggcga tctcgcagag cgaatgctcg ccgcactcga cgccgcggaa gcagccggcg
     3361 gcgatattcg cggacggcaa tcagcggcgc tgattgttgt caccggtaaa ccgaccggtc
     3421 aggcatggaa ggaccgcact ttcgatctgc gcgttgacga cagtccgcaa ccgttgaaag
     3481 agctgcgccg gctggtgcgt ttgcaacgtg cctacaacca catgaacgca ggcgacctgg
     3541 cagtcgaaaa gaaagacaac gaaggcgcgt tgcgcgagta ctccgccgcc gaacggctgg
     3601 tgcccgacaa tgcggagatg atctactggc atgccgtcgc gctcgtaaac atgggccgtg
     3661 tcgaggaatc tttaccgctg tttcgcaagg tgtttgcgat ggaccgcaac tggatcacgc
     3721 tgacaccgcg actccccaaa tccggcttac tgcccgacga tcccaagctc atcaaccgca
     3781 tcgtgtcggc gggaagcacg cgctcaaaca gacgttgacg gctttgtctt gacggctgcc
     3841 gcgttgtcgc ttataaaggg gaactctgat tcgccccata ccctcatagc cccttagt
//
LOCUS       DASTEY010000135         6133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1387413, whole genome shotgun sequence.
ACCESSION   DASTEY010000135 DASTEY010000000
VERSION     DASTEY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387413"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VFO72_02350"
     CDS             complement(<1..1471)
                     /locus_tag="VFO72_02350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9478156.1"
                     /translation="MVKDSPIAPKPQEHREDAPPAWPEVQDGLAASSGLALLLVDGYQ
                     PPAIVISNNNSICQTFQSSPEYVKLCDPYCGAAYAQATKAKDTIEYKCHAGLQCFAKR
                     VELGSKRNLAVIGGRAFTRSTDYQQLIERFRTGDLQSLAQDQTFSNVIFAEAQRVEEL
                     AERVDRTTQRFHAAASNGSAKTKAKVQPETAAAPPKPDLEHEVRRLRTELEYRARITN
                     SLQHFLDRISCAEPAKTYNSIVANSRELLRSERVSLLVLNEDSNELILKATSGWVNDP
                     AEVGPIPAGEGVSGKVIDSGEPMIVTDVRREGRAPAPGERRYKTSSFISYPIIISGRK
                     VGVLNVTDKTGGGTFNEVDLGLLDIIGPQVALALERAEWQERAGEFQLMSITDSLTKL
                     PNRRYLEERLVEELNRSQRYGYPMSFLMIDIDDFKTYNDKNGHPAGDRALEITAQCLR
                     GALRAEDVAARYGGEEFCILLPQTGLAEAGLIADRIRRLV"
     gene            complement(1651..2685)
                     /locus_tag="VFO72_02355"
     CDS             complement(1651..2685)
                     /locus_tag="VFO72_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9478157.1"
                     /translation="MPAVTEATLIAIRDLRVHFDLGGNRVVKAVDGVTLDIYRGETLG
                     LVGESGCGKSTLGRAVLRLTELTSGQVFYRNNDLAHLSGRQLREQRRHLQIIFQDPYA
                     SLNPRMTVGQIVGEPLETFRLARGREAEARVQELLETVGLSKRFVKRYPHEFSGGQRQ
                     RIGIARALAVDPDFIVADEPISALDVSIQAQIMNLLEKLRREKNLTYLFISHDLRAIR
                     HVSDRVAVMYLGKLVEIADAKTIYVEPLMPYTKALISAVPVPDPEIEATRQRIMLEGD
                     VPSPINPPSGCPFHTRCPYAIDVCKEVVPQLVEIKPAHFAACIRISPEMPHIEDVPPG
                     SAPGLESHRS"
     gene            complement(2669..3676)
                     /locus_tag="VFO72_02360"
     CDS             complement(2669..3676)
                     /locus_tag="VFO72_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478158.1"
                     /translation="MILLEVRDLRTYFQTDDGLVKAVDGISFQLQRGETLGIVGESGS
                     GKSVTNLSIIRLVPDPPGKIVSGEVIFKGQDILSLPPDAVRRIRGRRIAMIFQDPMTS
                     LNPFMKISKQLMEMTQLHLGHTKAQAYEHAVRMLETVGIPDARARAGNYPHEFSGGMR
                     QRVMIAMALSCEPELLIADEPTTALDVTIQAQILELIKKLKQETGTSVILITHDLGVV
                     AGMTDHVIVMYAGKIFEQAKTKELFALPGNPYTKGLLRSVPDPTAEQGKLYQIPGLPP
                     DVAHLKPGCPFADRCDHAEDICRREFPPFVELTPDHHSLCHFANEVYAESIQSSRASR
                     Y"
     gene            complement(3673..4665)
                     /locus_tag="VFO72_02365"
     CDS             complement(3673..4665)
                     /locus_tag="VFO72_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478159.1"
                     /translation="MEPEKKISPEMAVAEADVRTEEAGEFVRGTSLWRDAWRRLLKNK
                     LAVFGLVVVVLVTIASITGPTLIKRTFGFTPDYIPSNDVTLIRSFPPFRGPNGEFSWK
                     HPMGTDNAGRDQLARVLQGGQISLFVGIIATLVSLVIGVSYGAISGYVGGRVDNLMMR
                     IVDVLYSLPYVILVIVLLSLFRSQTARGQLILLFVALGSVSWLTMARIVRGQVLSLKN
                     QEFVLAARATGVSAPKIIFRHLVPNTLGPVIVYATLTIPQIMLTEAFLSFLGLGVQAP
                     LASWGSLAADGIQNIAIFPWQLIFPGVTMALTLFSLNFLGDGLRDALDPQMRTI"
     gene            complement(4740..5666)
                     /locus_tag="VFO72_02370"
     CDS             complement(4740..5666)
                     /locus_tag="VFO72_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9478160.1"
                     /translation="MLRFIIRRLLITIPTILVVITITWGLIKLAPGNFYSTERSLPPA
                     IEQNIREKYGLDKPWYQQYAKTMWNIVRHLDFGTSLRYEGQSVNTIIRRSLPVSAAVG
                     ILAYLLALIAGIAIGSIAALKQNSRWDYTSMALAMLGISIPNFVLGPILVLIFSLTLY
                     WLPPSRWGGFPSKNLILPVLTLSAIYMAYIARLTRAGMLEVLRSDYIRTARAKGLSEK
                     GVVIKHALRGGLMPVVSYTGPALAFLLTGTVGVEQIFALPGLGNYFIQASLNRDEPLI
                     IGIVAFIAIALLFMNLLVDIAYAYLDPRVRYH"
     gene            complement(5677..>6133)
                     /locus_tag="VFO72_02375"
     CDS             complement(5677..>6133)
                     /locus_tag="VFO72_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9478161.1"
                     /translation="QAQWKQNLGITVTLRNMDWKTFLQAKSKLDYKGFARGAWGADYM
                     DPFTFLGIFYTAEESGTGWSDPKYVALLDQANRTLDPKKRYELMAEAEAFMLKAQPVI
                     PIDTASVNWVKKPYVKGMYPNPASLFAWKYVYIERDQAKWDFGTPSLAE"
BASE COUNT         1399 a         1725 c         1683 g         1326 t
ORIGIN      
        1 tcacgagacg cctgatgcga tccgcgatta gtccagcttc ggcaaggccg gtttgcggca
       61 ggagaatgca aaactcctcg ccgccgtaac gcgcagcaac gtcttcagct cgaagcgcgc
      121 ctcgtaagca ctgagcagta atttccagag cgcgatcgcc tgccgggtgt ccgttcttat
      181 cgttataagt cttgaagtcg tctatatcga tcatcagaaa gctcatcggg tagccgtaac
      241 gctgagagcg attcaactcc tccacgagac gctcttcgag ataccgccgg ttcggcagct
      301 tcgtcagcga atcggtgatg gacatcagtt gaaactcacc ggcgcgttcc tgccattcgg
      361 ctcgttcaag cgcaagtgct acttgtggcc caatgatgtc gagtaatcct agatcgactt
      421 cattgaatgt gccgccaccg gttttgtctg tgacattcaa aactccgact ttgcggccgc
      481 tgataatgat cggataactg ataaacgaac tcgtcttgta acgccgctct cccggagccg
      541 gcgcgcgtcc ttcacgacgg acgtcagtca caatcatcgg ctcgccagaa tcgattactt
      601 ttccagatac tccttcgccg gccggtattg gtccaacttc ggctggatcg ttaacccacc
      661 cgctcgttgc tttcaagatc aactcgttcg agtcttcgtt cagcaccagc agcgacacgc
      721 gctccgaccg caacaattcc ctggagttgg cgacgatcga gttataagtc ttcgccggct
      781 cggcgcaact gatgcgatcg agaaagtgct gcaacgagtt ggtaatgcgc gcgcgatact
      841 cgagttcggt ccgcaaccgg cgaacttcgt gttcgagatc cggtttaggg ggcgccgcag
      901 ccgtttccgg ttgcactttc gctttcgttt ttgcggatcc gtttgaggct gcggcgtgaa
      961 aacgttgcgt ggttctgtct actcgctccg caagctcctc gacgcgttgt gcttcagcaa
     1021 agatgacatt cgaaaacgtt tgatcctgcg cgaggctttg cagatcgccc gtgcggaagc
     1081 gctcgattag ttgctggtaa tccgtgctgc gtgtaaacgc gcgcccgcct ataactgcaa
     1141 gatttcgttt gctgcccagt tcaactcgtt tcgcaaaaca ctgaagcccg gcgtgacact
     1201 tgtattcgat cgtgtccttt gccttggtcg cctgggcata ggcggcgccg caatacggat
     1261 cgcaaagttt cacgtactcc ggcgagcttt gaaatgtctg acagatggaa ttgttgttgg
     1321 agatgacgat ggcgggcggc tggtaaccat caaccagaag caaggcaagg cccgaggagg
     1381 ccgctagccc gtcctggacc tccggccagg caggcggagc gtcttcgcgg tgctcctgag
     1441 gtttcggggc aatgggggag tccttcacca tcgtgttctg ggcattacaa acttcaagtc
     1501 cgtggcctgt aaagacttac cgcggaacca cgaagaactg agcttccatc atccgcacac
     1561 gaggagttta ccctagtgct aaacggcgaa tgccgcgggg atttcaacag ctgcggccga
     1621 tcaatacgct attgtaagtg cgaaaacggt tcaactgcgg tggctttcaa gaccgggagc
     1681 agagccgggc ggaacatcct cgatgtgagg catttccgga ctaatcctga tgcaggcggc
     1741 gaaatgcgca ggcttgatct caacaagctg aggaaccact tccttgcaaa catcgatcgc
     1801 atacgggcaa cgagtatgga aaggacatcc cgaaggcgga ttgatcggcg acggaacgtc
     1861 gccctccagc attatacgtt gtctcgtcgc ttcgatctcg gggtcaggta ccgggacggc
     1921 tgaaatcagc gccttggtgt aaggcattaa gggctcgacg tagatcgtct tggcgtccgc
     1981 gatctccacc aacttgccaa gatacatcac cgccacgcgg tcggatacgt gcctgatcgc
     2041 ccgcaggtcg tgtgagatga agagataagt gaggttcttc tcgcgccgca acttttcgag
     2101 caggttcatg atctgcgcct gaatcgaaac gtccagcgcc gagatcggtt cgtcagcaac
     2161 gatgaaatcc ggatcgaccg caagcgcgcg agcaatacca atccgctggc gttgccctcc
     2221 tgaaaactcg tgcggataac gtttgacaaa cctcttactc agacccactg tttcgagcag
     2281 ttcctgcact cgcgcttccg cttcccttcc tcgcgccagg cgaaatgttt ctaacggctc
     2341 accgacgatc tggcccacag tcatgcgcgg gttcagcgac gcgtacggat cctgaaagat
     2401 tatttgcagg tggcggcgtt gttcgcgtaa ttgcctgccc gacagatggg ccaggtcgtt
     2461 attgcgatag aagacctgtc cactcgtcag ttccgtgaga cgcaacacag cacggccgag
     2521 cgtcgactta ccacagccgg actcaccaac cagcccgaga gtctcgcccc ggtagatgtc
     2581 gagcgtgact ccgtccactg ctttgacgac gcgattgcca ccgagatcga agtgcaccct
     2641 caaatctcga atggcgatca gggttgcttc agtaacggct ggcacggctt gattggattg
     2701 actcggcata gacttcatta gcgaagtggc acagcgaatg atgatcgggt gttagctcca
     2761 caaacggcgg aaactcgcgc cggcagatgt cttccgcgtg atcgcagcga tcggcgaacg
     2821 ggcaacccgg tttcaaatgg gctacgtccg gcggcaatcc tggaatctga tagagcttgc
     2881 cttgttcggc tgtcgggtcg ggcaccgagc gcagcaagcc ctttgtgtac ggattaccgg
     2941 gaagcgcaaa caactccttc gtttttgcct gctcaaaaat ctttccggcg tacatcacga
     3001 tcacgtgatc agtcatgccg gctacaacgc caagatcgtg tgtgatcagg atgacgctcg
     3061 ttcccgtctc ttgtttaagt ttcttaatga gctccaggat ctgcgcctga atagtgacgt
     3121 cgagcgcggt tgtcggttcg tccgcgatca gcagttcggg ttcgcaggac agcgccatcg
     3181 caatcatcac gcgttggcgc atgccgccgg aaaactcgtg cggataattg ccggctcgcg
     3241 cgcgtgcatc aggaatgccg acagtctcga gcatgcggac ggcatgctcg taagcctgcg
     3301 ctttcgtgtg gcccaggtgc agttgcgtca tctccatcag ttgtttggag atcttcatga
     3361 aaggattgag cgaggtcatc ggatcctgga aaatcatcgc gatgcgccgg cctctgattc
     3421 tgcgcaccgc gtcaggcggc aacgagagga tgtcctgtcc tttgaagata acttcacccg
     3481 aaacgatctt gcctggggga tccggaacga gacgtatgat cgagaggttt gtaacggatt
     3541 ttcccgaacc ggattctccc acaataccga gcgtttcgcc gcgctggagt tgaaacgaga
     3601 tgccgtcgac ggcttttacg aggccatcgt cggtttggaa gtacgtgcgg agatctctga
     3661 cttctaaaag gatcaaatgg tcctcatctg cggatcgagc gcgtcccgca agccatcacc
     3721 taagaagtta agtgaaaaca acgtcaacgc catcgtcaca cccggaaaaa tcaattgcca
     3781 ggggaagatc gcgatgttct ggatgccgtc ggcagcaaga ctgccccacg aagccaacgg
     3841 cgcctgcacg cctaaaccga gaaacgaaag aaacgcttcc gtcaacatga tctgcggaat
     3901 cgtcaaggtg gcgtacacga tcaccgggcc gagagtgttt ggcaccaggt ggcgaaagat
     3961 gatcttcggc gctgacacgc ccgttgcgcg tgcggcaagc acgaactcct ggttcttcaa
     4021 cgacagcacc tgcccgcgta cgatccgcgc catcgtcaac cacgaaacag aaccgagcgc
     4081 gacaaacagc agtatcaact gacctcgggc ggtctgactg cgaaacagcg aaagcagcac
     4141 gatcacgagg atcacgtacg gtaacgaata gagcacgtcg actatgcgca tcatcaagtt
     4201 atcgacgcgc ccgccaacgt aacctgagat tgcgccataa ctgacaccaa tcacaagcga
     4261 gacaagagtc gcaatgattc ctacgaacag agagatctgc ccgccctgca gcacacgcgc
     4321 gagttgatcg cgtcctgcat tgtcagtgcc catcgggtgt ttccaggaaa actcaccgtt
     4381 gggcccgcga aacggcggga acgatctaat cagtgtcacg tcattactgg gaatgtagtc
     4441 aggagtaaaa ccgaacgtgc gcttgatcaa agtgggtccc gtgatggaag caatggtgac
     4501 gaggaccacg acgacgaggc caaacaccgc gagcttgttc ttgagtaagc ggcgccacgc
     4561 gtctcgccac aacgacgtgc cgcgcacaaa ctcgcccgct tcttccgtcc gcacgtcggc
     4621 ttcggcaacc gccatctccg gactgatctt tttctctggt tccatcatgc tgctcctctg
     4681 tgcttctgtg tctcggtggt tttcgacaac cacaaagaca cagaggcaca gagacttaat
     4741 tagtgatatc tcacccgtgg atcgaggtac gcgtaagcaa tgtcaaccaa cagattcatg
     4801 aacaacaacg cgatcgcaat gaacgcgaca atgccgataa tcagcggttc atcccgattg
     4861 agcgacgcct gaataaagta gttccccaaa cccggcaacg cgaaaatttg ctctacaccg
     4921 acagttcccg tcagcagaaa cgccagtgcg ggaccggtgt acgagactac cggcatcaaa
     4981 ccaccacgca acgcatgttt gatgacgacg cccttttcac tcaaaccttt tgcgcgcgca
     5041 gtgcgaatgt aatctgagcg cagcacttcc aacatgccgg cacgtgtcag gcgcgcgatg
     5101 taagccatgt agatcgctga caacgtgagc accggaagaa tcaggttctt actcggaaag
     5161 ccgccccagc gtgaaggcgg caaccagtag agagtcaacg aaaagatcag cacgagaatt
     5221 ggaccaagta cgaagtttgg aattgaaatc ccaagcatcg ccagggccat tgacgtgtag
     5281 tcccaacgcg agttctgttt gagcgcagcg atcgaaccga tcgcgatacc ggcaatcaac
     5341 gccagcaggt aagcgagaat cccgacggcg gcagaaaccg ggagcgaacg ccggatgata
     5401 gtgtttacag actgtccttc gtatctcaga gacgtaccga aatcgaggtg ccgcacgata
     5461 ttccacatgg tcttggcgta ttgctggtac caaggcttgt cgagtccata cttctcgcga
     5521 atgttctgct cgatggcggg aggcaggctt cgctcagtgg aatagaagtt accgggagca
     5581 agttttatca agccccaggt aatggtaatc acgaccagga tcgtagggat cgtgatcagg
     5641 aggcgtctaa ttatgaagcg aagcattttc gcgttgctat tcggccaaac tcggtgtccc
     5701 gaagtcccac ttggcctgat cgcgttcgat gtagacgtac ttccaggcaa acaacgacgc
     5761 cggattcgga tacattcctt tgacgtacgg tttcttcacc cagttgacgg aagcagtgtc
     5821 gattggaata actggttgtg ctttaagcat gaatgcctcg gcttcggcca tcaactcgta
     5881 acgcttcttt ggatcgagtg tgcggttggc ctggtcgagc aacgccacgt atttcggatc
     5941 gctccagccg gtgccgctct cttcagcagt gtagaaaata ccgaggaacg taaacggatc
     6001 catgtagtcg gcaccccagg cgccgcgtgc aaatcctttg tagtcgagct tgctcttggc
     6061 ttgcaagaat gttttccagt ccatgttgcg cagcgtgacg gtaatgccga gattctgttt
     6121 ccactgcgcc tgc
//
LOCUS       DASTEY010000136         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_134896, whole genome shotgun sequence.
ACCESSION   DASTEY010000136 DASTEY010000000
VERSION     DASTEY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134896"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFO72_02380"
     CDS             <1..305
                     /locus_tag="VFO72_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478162.1"
                     /translation="VQSIDAASRNGEFLVLRTPPGDDFPTLCDSILGLLSANPGECEV
                     ALEALTDNGTIVRVKANQALRVKLSSELEEALKKLGCSVSVENASNFNGGGNGARV"
     gene            324..1217
                     /locus_tag="VFO72_02385"
     CDS             324..1217
                     /locus_tag="VFO72_02385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET9478163.1"
                     /translation="MAKGKDGKAQPEPAPPAGEVRTAFERVQLARHANRPYTLDLIER
                     MFEDFVELHGDKRFGEDPAIVAGYARFHGLPVVVVGHQKGRDTKQRSYRNFGMPKPEG
                     YRKALRVMKLGEKFGRPIFTFIDTPGAYPGIDAEERGQAEAIAYNLREMAKIKVPIIV
                     TVIGEGGSGGALAIGVGDQVLMLENAIYSVISPEGCAAILWKDSAKADLAAEGLRLTA
                     QHLFDEGIIDKIIPEPPGGAHNDYDQTARFLDSALSERLAEAVSCSQEDRLSRRYSKL
                     RRFGRWGTGDQNGISSSDKSA"
     gene            complement(1181..1606)
                     /locus_tag="VFO72_02390"
     CDS             complement(1181..1606)
                     /locus_tag="VFO72_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9478164.1"
                     /translation="MSFNKITIVGNLGRDPELRYTAQGTPVCTFSVATNERRKDRNGE
                     MQDHTTWFRVTLWNRQAETASQYLQKGRQVYIEGRLRVEEYVDRDGKPRHSLEVHGTD
                     MQFIGARGDDMPVERAASASASQGGPTDQADLSDDDIPF"
     gene            1751..3118
                     /gene="miaB"
                     /locus_tag="VFO72_02395"
     CDS             1751..3118
                     /gene="miaB"
                     /locus_tag="VFO72_02395"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9478165.1"
                     /translation="MKPRVYIETFGCQMNVADTERAATGLRSSGYELCDSADEADVVL
                     LNTCSVREKAERKVYTRIGEVRGARPGKNDVLVGVMGCVAQLEGGSVFDHAPSVDMVI
                     GTRATDRIPLLIERAQHGERKVMDLGERGEADSWDVSPAERHSPHVAFIPIIEGCNKF
                     CSFCIVPYSRGRERSRPASEIIAEVRNLQALGYKEIHLIGQNVNSYRPRVEDGLERFK
                     GATPFCRLLRAVAATGMPRIKFTTSFPRDFHGDIVTAIEENPNLCDWIHLPVQSGSDR
                     VLKLMRRGHTRDNYWQRIERIKNSSRRLALTSDIIVGFPGETEKDFHQTLDLVAQCEY
                     DSVYIFKYSKRAGTPAADFTDDVPEKEKTARFLALEEVQRKNQERIYRSYVGRTLQVL
                     VEKQSTRSESDLSGHSTCNKVVNFAGDRSLEGEIVEVTVTAAKTNSLYGAVGKSVMVN
                     RSAVV"
BASE COUNT          719 a          843 c          936 g          693 t
ORIGIN      
        1 aggtgcagag catcgacgct gcttcccgta atggtgagtt tctggtgttg cgcactccgc
       61 cgggagacga cttcccgacg ctatgtgatt cgattctcgg tttgctgagc gccaacccgg
      121 gtgaatgtga agtcgcgctg gaagcgctga ccgacaacgg gacgattgtg cgggtcaaag
      181 ccaaccaggc gttgcgcgtt aagctgagta gcgagttgga agaggcgctt aagaaattgg
      241 gctgtagtgt cagtgtagag aacgcatcaa acttcaacgg aggcggcaac ggcgcgcgcg
      301 tctgataacg acggaagagt caaatggcaa agggcaaaga cggaaaggct caacctgaac
      361 ccgctccacc ggcgggtgaa gtgcggactg ctttcgagcg tgtacagttg gcgcgtcatg
      421 cgaatcgccc gtacacgttg gatctgatcg agaggatgtt tgaagatttc gttgagcttc
      481 acggcgacaa gcgtttcggc gaggatccgg caatcgttgc cggttatgcg cgctttcacg
      541 gtttgccggt cgtcgtcgtc ggtcatcaga agggacgcga cacgaagcaa cgcagctatc
      601 gcaacttcgg catgccaaag ccggaaggct atcgcaaggc gctgcgcgtg atgaagctag
      661 gtgagaagtt tggccgtccg attttcactt tcatcgacac gcccggcgcg tatcccggta
      721 tcgatgcaga agaacgcggc caggcagagg cgatcgctta caacctgcgt gaaatggcca
      781 agatcaaggt gccgattatc gtgactgtta ttggagaagg cggatcgggt ggtgctttgg
      841 cgattggtgt gggtgatcag gtgttgatgc tcgagaacgc catctattca gtgatcagtc
      901 ctgaaggttg tgcggcaatt ctctggaagg attcggcgaa agccgatctc gccgccgaag
      961 gtctgcgact aaccgcgcag caccttttcg acgaaggcat tatcgataag atcatccccg
     1021 aaccacccgg cggcgcgcac aacgactacg atcaaactgc gcgctttctc gactctgcct
     1081 tgagcgagcg tttagcggaa gcagtcagtt gttcgcagga agatcgactc agtcgccgtt
     1141 acagtaagtt gcgccgcttt ggtcgttggg ggaccggaga tcaaaacgga atatcgtcgt
     1201 cggacaaatc cgcctgatcg gttggcccac cctgtgatgc gcttgccgat gcggcgcgtt
     1261 caacgggcat atcgtcaccg cgcgccccga tgaactgcat gtcggttccg tgcacttcga
     1321 gcgagtgacg tggtttgccg tcgcgatcga cgtactcttc aacgcgcaaa cgtccttcga
     1381 tatacacctg acgtcccttt tgcagatact gtgaggcagt ctctgcctgg cggttccaca
     1441 acgtcacgcg aaaccacgtc gtgtggtcct gcatttcacc gtttctatct ttgcgccttt
     1501 cattcgtggc cacgctgaag gtacaaactg gggtaccttg agccgtgtag cgcaactcgg
     1561 gatcgcgacc gagatttccc acgatagtta ttttgttaaa ggacatgagg agcctttctt
     1621 ttataggatt acgaacgttc gcgatactac tagcgccgca aacaagaatc aagcaactcc
     1681 catttctaat cgcgatttat ctcggctagt gattctggta gactgtggca accttaagcg
     1741 gtttggaaga atgaagccac gagtttacat tgagaccttc ggctgccaga tgaatgtcgc
     1801 cgacacggag cgcgcggcca ctggtttgcg cagttcgggt tatgaacttt gcgactcggc
     1861 agacgaagca gacgtggtcc tgctcaacac ctgttcggtg cgcgagaagg ccgagcggaa
     1921 ggtctacacg cgcatcggcg aagtccgcgg agcgcgtccg ggtaagaacg atgttctggt
     1981 cggtgtaatg ggctgtgtgg cgcagctcga aggcgggtcc gtcttcgatc acgcgccctc
     2041 cgtcgacatg gtaatcggga ctcgtgcaac tgatcgcatc ccactgctga ttgaacgtgc
     2101 tcagcacggc gaacggaaag tcatggatct cggcgagcgt ggcgaggctg attcgtggga
     2161 tgtctcgccg gctgaaaggc attctccaca cgtcgctttc attcccatca tcgaaggttg
     2221 taacaagttc tgttcgtttt gcatcgtccc ttactcgcgt ggccgcgaac gcagccgccc
     2281 ggcaagcgag atcatcgccg aggtgagaaa tcttcaggca cttggttata aagagattca
     2341 tctcatcggt cagaacgtca acagttaccg gcctcgtgtc gaagacggtc tggaacgttt
     2401 caaaggcgcg acgccattct gccgcctgtt gcgtgcggtt gccgcgaccg gaatgccgcg
     2461 aatcaagttt acgacctcgt ttccgcgcga ctttcacggc gacatcgtta ccgcaatcga
     2521 agagaaccca aacctttgcg actggattca cctgccggtg caatcaggca gcgaccgggt
     2581 cctgaaactg atgcgtcgcg gtcacacgcg cgacaactac tggcagcgca tcgaacgtat
     2641 caagaactcc agccgtcgtc tcgcattaac gagcgacatc attgtcggct ttcccggtga
     2701 aaccgaaaaa gactttcacc agacgctcga tctagtcgca cagtgcgagt acgacagtgt
     2761 ttacatcttc aaatactcga agcgagccgg aacccctgcg gctgacttca ctgacgacgt
     2821 gccggagaaa gaaaaaacgg cgcggttcct ggcgcttgaa gaagtacagc gaaaaaacca
     2881 ggagcgaatt tatcgatcgt acgtcggtcg aacattgcag gtgctcgtcg agaaacagag
     2941 caccagatcc gaaagcgatc tctcggggca ctccacgtgc aataaggtcg ttaactttgc
     3001 cggcgatcgc agtctcgaag gcgagatagt cgaggtcact gtgaccgccg ccaagacaaa
     3061 cagtctgtac ggggccgttg gtaaatcggt catggtaaat cgttcagcgg tagtgtaagg
     3121 ggtacctgcg ttatggaaat cgaggttaag atccgagcac taatgatgga tccgaatagc
     3181 ggaactccaa t
//
LOCUS       DASTEY010000137         3673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1140259, whole genome shotgun sequence.
ACCESSION   DASTEY010000137 DASTEY010000000
VERSION     DASTEY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140259"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFO72_02400"
     CDS             complement(<1..362)
                     /locus_tag="VFO72_02400"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9478166.1"
                     /translation="MTDFRNSPLAEVDPVIAGAIDNEVARQANGLELIASENFVSEAV
                     LEAAGSVFTNKYAEGYPGKRYYGGCEWTDVVEQTAIDRAKELFGAEHANVQPHSGAQA
                     NMSVYLAAIQYGDQILGM"
     gene            complement(359..805)
                     /gene="rpiB"
                     /locus_tag="VFO72_02405"
     CDS             complement(359..805)
                     /gene="rpiB"
                     /locus_tag="VFO72_02405"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9478167.1"
                     /translation="MRIALASDHAGYAEKERLKTVLTELGLDFEDLGPASDASVDYPD
                     YARRVAEQVAQGQVEQGLLVCGSGTGMAMTANKVHGVRAAVAWSEETARLARQHNDAN
                     VLALGARTTPSAEIPKIVRAWFSTDFEGGRHAARVAKICEVERSYK"
     gene            929..2674
                     /locus_tag="VFO72_02410"
     CDS             929..2674
                     /locus_tag="VFO72_02410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9478168.1"
                     /translation="MRWSSFFIPTLREDPADAEVASHKLLLRAGVIRQLSAGIYSLLP
                     LGQRVALKVMQILREEMNAIGGQEFYLPALHPAEIWQESGRWSAIGEEMFRLKDRKGA
                     DMCLGMTHEEIFTSIARHEIRSYKQLPQVWYQIQIKFRDEARPKSGLMRLRTFIMKDA
                     YTFDVDRAGLDKAFLDQREAYKNIFTRCGLQFLIVEASSGSMGGSESSEFIAPSEAGQ
                     DLIVSCRNCGYAANLEKAISRLAPISDDAGPAAPEEFPTPGVRTIDDLITFPGGAEAA
                     RQIKSLVFFATIEGEQRPVLVLLRGDHQLHETKLADGLRATAVRPAHPEEITELLGAG
                     AGSLGGVGAKEKARAARAVLRIVADSALQNRRNMTTGANKDDHHLRGVDVTRDIQVDE
                     WLDLRTVESGEGCPRCETGVLEVFKGMEIGHIFKLGTRYSETMGATVLNQEGKEVPIY
                     MGSYGIGVERIISAAVEQRNDEDGIIWPKSIAPFDVVLTVTNMKQDELREAGEKLYHD
                     LQRAGLEVLLDDRDERAGVKFKDADLIGIPYRITIGKKAAEGLVELFDRHKKQSEDVR
                     IGDVIARVQRLALSG"
     gene            2683..>3673
                     /locus_tag="VFO72_02415"
     CDS             2683..>3673
                     /locus_tag="VFO72_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9478169.1"
                     /translation="MKLVWLLLALIIPLRVEAAQDILPELVRRIKPSAVAIETFDSRG
                     EKLSRGSGFFIDTDRIVTNRHVLEGAHRAEVHTSNGTVFPVKGVLAVDAEGDIALLKI
                     DAPSPPIRPLPLDKTSPQEGESVVVIGNPLGLEGSVTNGIVSAVRDIPTFGRIIQITA
                     AISSGSSGSPVVNMQGQVIGIATLQVTGGQSVNFAIPSERISQLQIASLMSLGDLVAT
                     SGRNKRAKAVQFFRDGLSFLSKDDCEKALPYFEKAVESDSSYADAWAQAGFCNEKLGK
                     HAEALEASKKAVSLRPSAESYFNIGLASFYLKQYKDAAEAYRAAIKMDPYNSAD"
BASE COUNT          812 a         1025 c         1060 g          776 t
ORIGIN      
        1 ctcattccga gaatctgatc gccgtactga atagcggcga gataaacaga catgttggcc
       61 tgcgcaccac tgtgcggctg cacgttcgca tgttcggccc caaacaattc tttggcgcgg
      121 tcaatcgcgg tctgttctac gacgtcggtc cactcgcaac cgccgtaata tctcttgccc
      181 ggataacctt cggcatactt gttcgtgaac accgaaccgg cagcttcaag cactgcttca
      241 ctgacaaagt tttccgacgc gatcagttcc aatccgttgg cctggcgtgc gacttcgtta
      301 tcgatcgcgc cggcaattac cggatccacc tcggccagag gcgagtttcg aaaatcagtc
      361 atttgtagct tcgctccact tcgcaaatct tcgccacgcg cgcagcgtga cggccacctt
      421 caaaatctgt cgagaaccac gcgcgcacga tcttcggaat ttcggcggac ggcgtagtgc
      481 gtgctccgag cgcgagtacg ttggcgtcgt tgtgttgacg cgcaagccgc gccgtctctt
      541 cagaccacgc gaccgcagcc cgcacgccgt gaactttgtt ggcggtcatg gccatgcccg
      601 tgccggagcc gcaaacgagc agtccctgtt cgacctgacc ttgcgcaacc tgctccgcca
      661 cccggcgagc gtagtcggga tagtcgaccg acgcgtctga cgcggggccg aggtcctcaa
      721 agtcgagacc gagctccgtc agcaccgtct tcaaccgctc cttttcagcg taaccagcgt
      781 gatcgctggc gagagcaatt ctcatcgtcg gataaccttc gtgaagggat gttcaagaaa
      841 gaacgtgatg gtaacgcact ctaatccgcg cggcaaattt caccttcatt tgccttactc
      901 gtagccgcta agcgagaatg caccgtctat gcgctggtca agtttcttta tccctactct
      961 ccgtgaagat ccggcagacg ccgaggtcgc ttcacacaaa ctactgcttc gcgcaggtgt
     1021 tattcgtcaa ctttccgcgg gcatctattc tttattgccg ttgggccaac gtgtcgccct
     1081 caaggtgatg caaattctcc gcgaagagat gaacgcgatc ggcggccagg aattttatct
     1141 gccggcgctc cacccggccg aaatctggca ggaatcgggc cgctggagcg ctatcggcga
     1201 agagatgttt cgcctgaagg accgcaaagg cgcggacatg tgtctgggca tgacgcacga
     1261 agagatcttt accagcatcg cgcgccatga aattcgttct tacaaacaac tgccgcaggt
     1321 ttggtatcag atccagatca agtttcgtga tgaagcgcga ccaaaatcgg gtttgatgcg
     1381 gttgcgcacc ttcatcatga aagacgccta cacctttgac gtagaccgcg ccggactcga
     1441 caaggcgttt ctcgatcaac gcgaagctta caagaatata tttacgcgct gcggactaca
     1501 gtttctgatc gtcgaggcct cgtcgggatc gatgggcggt tcagagtcga gtgagtttat
     1561 cgcaccatca gaggccggcc aggacctgat cgtcagttgt cgaaactgcg gctacgccgc
     1621 aaatctcgaa aaggccatct cgcgcctggc gccgatcagt gacgatgccg gccccgctgc
     1681 gcccgaggag tttcccactc caggcgtacg gaccatcgac gacctgatca cttttcctgg
     1741 gggcgctgaa gcagcgcgcc agatcaagtc gctggttttc tttgcgacga tcgagggcga
     1801 acagcgtccg gtgctcgtgc tgctccgcgg cgatcatcaa ttgcacgaga caaaactggc
     1861 cgacggcctt cgcgcaactg cggtgcgtcc ggcgcatccc gaagagatca ctgagctact
     1921 cggagcaggt gccggaagcc tgggtggagt tggagcgaaa gagaaagctc gtgctgctcg
     1981 tgctgttttg cgtatcgttg cagactcggc gctacagaac cgccgcaaca tgaccaccgg
     2041 tgcaaacaaa gacgaccatc acctgcgcgg tgtcgacgtc acgcgcgata tacaagttga
     2101 cgagtggctc gacctgcgca ccgtcgaatc gggtgaagga tgcccgcgtt gcgaaacggg
     2161 cgtactcgaa gtcttcaaag ggatggagat tggtcacatc ttcaaactcg gaacgagata
     2221 ctctgagacg atgggcgcca ccgtgctgaa ccaggaaggg aaggaagtac cgatctacat
     2281 gggcagctac gggatcggcg ttgaacgtat catcagcgcg gccgtcgaac agcgaaacga
     2341 tgaagatgga atcatctggc cgaagtcgat cgcgccgttt gatgttgtgt tgacagtcac
     2401 gaacatgaaa caggacgaac tgcgtgaagc cggagagaag ttgtaccacg atctacagcg
     2461 tgcgggtctc gaggtgttgc tggacgaccg cgacgaacgc gccggtgtaa agttcaaaga
     2521 cgctgacttg atcggcatac cttaccggat aacgatcggg aaaaaagctg ccgagggcct
     2581 agttgaactg ttcgatcgcc acaaaaaaca gagtgaggat gttagaatcg gcgacgtcat
     2641 agcgcgcgtg cagcggctgg cactatcagg ttgagagcag aaatgaaatt agtctggctc
     2701 ctactagcct taatcattcc cctaagagtc gaagcagcgc aggatattct cccggaactc
     2761 gtccgccgca ttaaaccctc cgcggtagca attgaaacgt ttgattcgag gggtgaaaag
     2821 ctctcgcgcg ggagtggttt ctttattgac acggatcgta tcgtgacgaa ccgtcacgtg
     2881 ctcgaaggcg ctcatcgcgc ggaagtgcat acttcaaacg gaaccgtttt tcctgtcaaa
     2941 ggtgttctcg ctgttgacgc ggagggcgat atcgcactgt tgaagattga cgcgccatca
     3001 ccgccgatca ggccgttgcc gctcgataag acttcgccgc aggaaggcga gagcgtcgtc
     3061 gttatcggta acccgctcgg tttggaaggc agcgttacga acggaatcgt ttcggccgtg
     3121 cgcgacattc ccaccttcgg acgcatcatt cagatcaccg ccgcgatctc gtcggggtcg
     3181 agtggcagtc ccgtggtgaa catgcaggga caggtcatcg gcatcgcgac gctccaagtc
     3241 actggcgggc agagtgtgaa cttcgctatt ccttccgaac gcatttcgca actgcaaatc
     3301 gcttcattga tgtcgctggg ggatttggtg gcgacgtccg gtcgcaacaa gcgcgcgaaa
     3361 gcggtgcagt tcttccgtga tgggttgagt tttctttcga aagacgattg cgaaaaggcg
     3421 ctgccgtatt ttgaaaaggc ggttgagtca gacagtagtt acgcggacgc gtgggcgcag
     3481 gccgggttct gcaacgagaa gctcggcaaa cacgcggagg ctttggaagc ttcgaaaaag
     3541 gcagtcagcc tgcggccctc ggctgagtcg tacttcaaca tcggtctcgc cagtttttat
     3601 ctgaagcagt ataaggacgc cgcggaagct tatcgcgcag ccattaaaat ggatccgtat
     3661 aactcggcgg acg
//
LOCUS       DASTEY010000138         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1140268, whole genome shotgun sequence.
ACCESSION   DASTEY010000138 DASTEY010000000
VERSION     DASTEY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140268"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFO72_02420"
     CDS             complement(<1..1246)
                     /locus_tag="VFO72_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9478170.1"
                     /translation="MSVTKYKTLVPDIEFWMGMIPCQAACPVKTDAGKYVQLIAEGKF
                     KQSYLTARSPNPFASVCGRVCAAPCEDACRRGRIDAPITIRALKRFVTEKYGVESMEP
                     DTQDDLREGVVDEGNKRRGHLPVLQATRANVARGQKVAVIGAGPAGIAAAHDLALMGY
                     RVTVFEATNQAGGMMYHGIPEFRLARSIIEKEIQKVVQLGVEIKLNTPLNDKFGINEL
                     KAAGFESVFLSVGVQKGRDMNIEGSNLDGVIKAIDYLLNINNGYRVDLGKRVLVIGGG
                     FVAFDAARMALRAGLAAGDSSSGEAPAGNEGLHAAIDAARTAIRAGAIEVHIASLESF
                     QEMPVMRTTQGREEFEEAQHEGVRFLTQRGAKRFVGDNGRLKAVEFIGVKRTYDDDGR
                     FNPVYDPELSETFEADSVILAIG"
     gene            complement(1640..2914)
                     /gene="hemG"
                     /locus_tag="VFO72_02425"
     CDS             complement(1640..2914)
                     /gene="hemG"
                     /locus_tag="VFO72_02425"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET9478171.1"
                     /translation="MIGTLNSSKKEATVVGAGISGMLAAYALDRNGYRVTLLEEKERA
                     GGLIQTRNTGHGIAESAAHSLIATRPVRDLCRELGVELVAPRKEAKAKYIVRDGKLCR
                     FPLTFGECVGTLTHAALGRSTSNDSQNLEFWARRHLGNVALDYLLTPFVRGLYGVQPA
                     ELGVATAFPSLDLPEGKTLLGAMLTKRRNGASTKEKKSRAAPRFGMGDLVQRLEQRLE
                     KRLGSRFHKGEKVDQLPEAANVIVATPAYAAARLIRPASDTLADKLAEVRYTPIVSVT
                     VFVDRRSFTRPLHGVGVLMPAREETKSLGILFNSSSFAHRVADESRFASFTVMMGGTA
                     QPHWLTASDDEISQAIKLELSAVLGIREPLETAIHRWPAALPQYSVNLRDVWACARES
                     WCAIPGRILFGNYTGQISLRGMIETSATLGCG"
BASE COUNT          653 a          888 c          818 g          694 t
ORIGIN      
        1 gcccaatcgc caggatcacc gaatctgctt cgaacgtttc gctcaattcc ggatcgtaaa
       61 ccggattgaa tcggccatcg tcgtcataag tacgcttgac gccgatgaac tcaacggctt
      121 tgagccgccc attgtcgccg acgaatcgct tagctccgcg ctgcgtcaga aagcggacac
      181 cttcatgttg ggcctcttca aactcttcgc gtccctgcgt ggtccgcatc accggcattt
      241 cctggaatga ttccagactt gcgatatgaa cttcgatcgc gccggcccga atcgcggtgc
      301 gcgccgcgtc aatcgccgca tgcagtcctt cgtttccggc gggtgcttcg cctgagctcg
      361 agtctcctgc tgccaggccg gcgcgcaggg ccatgcgtgc tgcatcgaag gcgacaaaac
      421 caccaccgat taccagaact ctttttccca aatcgacgcg ataaccgttg ttgatgttga
      481 gcaggtagtc gatcgccttg atcactccat cgaggtttga accctcgatg ttcatgtcgc
      541 gtcccttctg cacgccgacg ctgagaaaaa ctgattcgaa gcctgcggct ttcagttcgt
      601 tgattccgaa cttgtcattg agcggagtat tgagtttgat ctcgacgccg agttgaacga
      661 ctttttgaat ctctttctcg atgattgagc gtgcgagacg aaactcggga ataccgtgat
      721 acatcatccc gccggcctgg ttcgttgctt cgaagaccgt aactcgatag cccatcaaag
      781 ccagatcgtg cgccgcggca atgcctgctg gaccagcgcc gatgactgcg actttttgac
      841 cacgagccac gttggcgcga gtcgcctgaa gcaccggcag atgcccgcgc ctcttgttgc
      901 cttcgtcaac aactccttcg cgcagatcat cctgcgtgtc gggctccatg gattcgacgc
      961 catacttttc agtaacaaaa cgcttgagtg cgcgaatggt gatcggggca tcaattcttc
     1021 cccgccggca ggcgtcctcg catggcgcgg cacagacacg cccacaaacg gaagcaaagg
     1081 gattcggaga tctggccgta agataggatt gtttaaactt gccttcagcg atgagttgga
     1141 cgtacttgcc cgcgtccgtt ttgacgggac aagctgcttg gcaaggaatc atgcccatcc
     1201 agaattcgat gtcgggcacg agcgttttgt atttggttac actcatacct agggtcggtt
     1261 ttgtccgctc ggcgattcac aagcaagact tgctccaaat attgcgcccc tatttcgcga
     1321 tgtggctgag aggatggcag gcgcagaatg cgaaaacttc cgcaggactt gcgaagtttt
     1381 aacctatgac ttccgaactc ttactgtttg gctgggataa gcgactggcc gcgcttgtgc
     1441 agtttgaaaa ctcagacagc tttctacctg ggaccgcagg cgtctcgcct gcacggtgac
     1501 gagtagggcg ctgccgtgag atgcaggcga gacgcctgcg ttcccaggta aacggtccgc
     1561 actttcaaac tgcatcactt cgtcttggga taaacaacca ggaaaatcgt aatcacgccg
     1621 aagacgactt cgcccaatat caaccgcagc caagtgtagc cgaggtttcg atcatgccgc
     1681 gcaaactaat ttgtccggtg taattgccga agagtatacg gcctggtatt gcacaccagc
     1741 tctcgcgcgc gcaagcccaa acatcacgga gattgactga atactgcggc aacgcagccg
     1801 gccagcggtg aattgctgtc tcgagcggct cgcgaattcc caacacggcc gacagctcca
     1861 gcttgatcgc ctggctgatt tcatcgtcgg aggctgtgag ccaatgcggt tgcgctgtgc
     1921 cacccatcat caccgtaaac gacgcgaagc gagactcgtc tgcgacgcga tgagcaaatg
     1981 agctgctgtt gaatagaata ccgagcgatt ttgtttcctc gcgcgccggc attaatactc
     2041 caacaccgtg tagcggccgt gtgaacgagc gtcgatcgac aaaaaccgtg acggacacaa
     2101 tcggcgtata acgaacctcg gccagtttgt ctgccaaagt atccgaagca ggtctaatga
     2161 gtcgcgccgc cgcataagcg ggtgtggcaa cgattacgtt cgcagcttcg ggcaactggt
     2221 ccactttctc gcctttgtga aatctcgaac caagtcgttt ctcgagccgt tgctccagac
     2281 gttgcacgag atctcccatt ccaaagcgtg gcgcagcgcg actcttcttc tctttcgtag
     2341 acgcgccgtt gcgacgtttg gtcagcatcg ctccgagaag agtctttccc tcaggcagat
     2401 caagagaagg aaaagcagtt gccacaccca gctcggccgg ttgcacgccg tagaggccgc
     2461 gcacaaacgg cgtcagaaga taatccaacg ctacgttgcc gagatgtctc cgcgcccaaa
     2521 actcgaggtt ttgcgaatcg ttgctggtag agcggcccag cgccgcgtgc gtaagagtgc
     2581 cgacgcattc gccaaaggtc aacggaaaac ggcacagctt gccgtcgcgc acgatgtatt
     2641 ttgcttttgc ttctttgcgt ggcgctacga gttccactcc gagttcgcga cacagatcgc
     2701 gcacgggccg agtcgcgatt aacgaatgtg ccgcactctc ggctatgccg tggccggtgt
     2761 tgcgcgtttg aattaaccca cccgcgcgct ccttttcctc gaggagcgtc acgcgatagc
     2821 cgttacggtc caaagcgtag gccgcaagca ttcccgaaat accggctcct acaaccgtcg
     2881 cctctttctt actggagttt agtgtgccaa tcatgctgag gcctccatca caattgattt
     2941 cagcaccggc ggaaattgcg ggtcatcgtt aaaacaacgc gcgcggtagt agtgtctgat
     3001 gccgtgcttt cgcgctttct ctcgatagag ccggtcgagt tcaaaaagag ttt
//
LOCUS       DASTEY010000139         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1107271, whole genome shotgun sequence.
ACCESSION   DASTEY010000139 DASTEY010000000
VERSION     DASTEY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107271"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFO72_02430"
     CDS             <1..205
                     /locus_tag="VFO72_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478172.1"
                     /translation="LALDRNGNGTIDNGQELFGNFKPQPTPPLGIERNGFLALGEYDK
                     SANWPTCSMAPDRPHSHTIRTPK"
     gene            complement(258..333)
                     /locus_tag="VFO72_02435"
     tRNA            complement(258..333)
                     /locus_tag="VFO72_02435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Lys,seq:ttt)
     gene            513..974
                     /locus_tag="VFO72_02440"
     CDS             513..974
                     /locus_tag="VFO72_02440"
                     /inference="COORDINATES: protein motif:HMM:NF039957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HET9478173.1"
                     /translation="MTYAEFVRTPLIEGAIGGALLSLIAFLLSRFVRDIVGRTLLATV
                     LFAAAGAYLGFAFNPTTPRIWLLMELLQVIAFGTLGLYGWRGSPYWLALGWALHPVWD
                     FALHYVGPGNTFAPLPYTLACISFDWVVALYIVIAYRLLRLASRQAPSLAR"
     gene            complement(916..2250)
                     /locus_tag="VFO72_02445"
     CDS             complement(916..2250)
                     /locus_tag="VFO72_02445"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET9478174.1"
                     /translation="MSKVVAVIGAQWGDEGKGKIVDLLAEQFDIVARYQGGHNAGHSV
                     QVGDESFVLHLLPSGIVHRGKICVLGNGMVIDPKAFFVEADRLTQQGIEVSPERVKIS
                     SRAHLILPYHRALDHTSEERLGNEKVGTTLRGIGPAYEDKAGRRGIRTADALVPNVLR
                     SRIERNLEDANRIIVAYGGDPLSVDEIFNEMSVLTERLAPFIADTTHFLNIAAGKGRS
                     ILLEGAQATLLDVDHGTYPFVTSSTTVAGGAIIGTGLAPNRLSGVLGIVRTYTTRVGE
                     GPFPTEMLEGEAELGQMIRERGREYGASTGRPRRCGWFDAFATRYAAEINGFTSIALT
                     KLDVLDALDQIKVCTGYKLDGRLCESLPAVSQDLRRIEPVYATLPGWQTSTLGMTEMS
                     ALPRNARAYVEFLSSQIGVEIGLVSTGPERSQTIIVQDSALGGWLDAVAGKQ"
     gene            complement(2254..2694)
                     /locus_tag="VFO72_02450"
     CDS             complement(2254..2694)
                     /locus_tag="VFO72_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9478175.1"
                     /translation="MKPARTSLPFEARLARRVRARKVAGAKLRARQAQGKLRRFVITN
                     FRKEETIAALALRRGECNRCGACCELVFKCPFLKKNGDGTSLCGIYENRPNSCRLFPI
                     EPRDINEVRGTCSYYFIEKPLKSLPIVDFRLPISNRQSTIGNRQ"
BASE COUNT          630 a          835 c          835 g          656 t
ORIGIN      
        1 gctcgcttta gaccgtaacg gcaacggtac aatagataac ggacaagagt tgtttggcaa
       61 cttcaagccg caacctacac cacccctggg aattgagcgt aacggctttc tcgccttggg
      121 cgaatacgac aagtcagcaa actggcccac ctgctctatg gcacctgatc gccctcactc
      181 gcacactatt cgcacaccaa aataaatagg ggcagctctt cgacatgcaa gtggcagccc
      241 taaatcattg acgctattgg tgagccgagc aggactcgaa cctgcgaccc actggttaaa
      301 agccagttgc tctaccaact gagctatcgg cccatctaca tgcggagagc acgcgcgggc
      361 gtgctctttt caattgcgag gaacttcgac gcgcattcta cctaaccagg tctgtattgt
      421 ccaaagtggc gcttgatttc gccgccgctc tgagtagacc cagcgggcga agaggtggtt
      481 gaatctgaac cgaaatggaa ataccagcag gaatgacgta tgccgaattt gttcgaaccc
      541 cgctgattga gggcgctatc ggtggggcac tgctgtcgct gattgcgttt ctgctgtcgc
      601 ggtttgtcag agacatcgtt ggacgaacac tactggcaac ggtgctgttt gcagccgcgg
      661 gtgcgtatct cggatttgcg tttaacccca ccacgccgcg catctggcta ttgatggaac
      721 tactgcaggt gattgccttt ggaaccttgg gactgtacgg ctggcgaggc tcaccgtact
      781 ggctcgcact cggttgggca ttgcatccgg tctgggattt cgccctgcac tatgttggac
      841 caggcaacac cttcgccccg ttgccgtaca cgctcgcgtg tatcagcttc gattgggtgg
      901 ttgcgctcta catcgtcatt gcttaccggc tactgcgtct agccagccgc caagcgccga
      961 gtcttgcacg atgatcgtct gtgaacgttc cggaccagtg gaaaccaaac caatctcaac
     1021 gccaatctgc gacgagagaa actccacgta agcacgagca ttgcgcggaa gcgcactcat
     1081 ctccgtcatg cctaacgtcg aagtctgcca acccggcaac gtcgcataga ccggttcgat
     1141 acgtcgcagg tcctgtgaca ctgccggcag cgattcacac aaacggccat cgagtttgta
     1201 acccgtgcag actttgatct gatccagcgc atcgagcacg tcgagcttcg ttagtgcgat
     1261 tgaagtgaag ccgttgatct ccgcggcata acgcgtggca aacgcatcga accagccaca
     1321 acggcgcggg cgtcctgttg acgcgccgta ctcacgacca cgttcgcgga tcatctgacc
     1381 caactctgcc tcgccttcaa gcatttcggt cgggaagggt ccttcaccaa cgcgcgtcgt
     1441 gtaagtgcga acaataccga ggactccact caagcgattc ggcgcgagac cagtgccgat
     1501 gatcgcgccc ccggcaaccg tcgttgagga agtaacgaat ggatacgttc catggtccac
     1561 gtcgagaagc gtcgcctgcg cgccttcgag caggatcgaa cgacctttgc cggcggcgat
     1621 gttcaggaaa tgtgtcgtgt cggcaataaa cggagcaagg cgttcggtaa gcaccgacat
     1681 ttcgttgaag atttcgtcaa cgctgagtgg atcaccgccg tacgccacaa tgatccggtt
     1741 ggcgtcctcg agatttcgtt cgatgcgcga tcggagtacg ttaggtacga gtgcgtcggc
     1801 ggtgcgaatg ccgcgacgcc cggccttgtc ttcataggca gggccgatgc cgcgcaacgt
     1861 ggtgccgact ttttcatttc ctaaacgctc ttcgctcgtg tggtccaacg cgcgatggta
     1921 gggaagtatc aggtgcgcgc gcgaactgat cttcacgcgc tcgggtgaca cctcgatgcc
     1981 ttgttgcgtc aggcgatcgg cttcgacaaa gaaggctttc ggatcgatga ccatgccgtt
     2041 gccgagtacg cagatcttgc cgcgatgcac gatgcccgac ggaagcaggt gcaacacgaa
     2101 cgactcgtcg ccgacctgca ctgaatggcc cgcgttgtga ccgccctgat agcgcgcaac
     2161 gatatcgaat tgctcggcga gcagatcgac gatcttgcct ttgccttcat cgccccattg
     2221 ggcgccgatt actgcgacaa cttttgacat aggttattgc cgattgccaa ttgtcgattg
     2281 tcgatttgaa atcggcaatc gaaaatcgac aattggcaat gacttcaagg gcttttcaat
     2341 aaagtagtaa ctacaagtgc cgcgaacttc gttgatgtcg cgcggttcga tgggaaagag
     2401 gcgacacgaa ttcgggcggt tctcgtaaat gccgcagagg gaggtgccgt cgccgttttt
     2461 cttcaggaac ggacatttga agacgagttc gcagcaagcg ccgcaacggt tgcattcgcc
     2521 gcgtcgcagc gcgagtgccg cgatggtctc ttctttacga aagtttgtga taacgaagcg
     2581 gcgcagtttg ccctgggcct ggcgggcgcg cagttttgcg ccggcgacct tacgcgcgcg
     2641 aacgcgcctg gcgaggcgcg cttcgaaggg gagagatgtg cgagccggtt tcattaatgc
     2701 gtcgagttag cgaacgcttt cagcgtttga ctccttggtt aatgacggta acccagggtt
     2761 gaagcaaccc tgagcgggac cttgcgaacg ccgttggcgt tgtaaagcaa cgaatcacgt
     2821 gcaaagtttc caagtcatcg gctttgcctc aaaagtcttc tttcattctc accgggcttc
     2881 agcccggtgt taccgccacg cggttgtgag aaaccgcttt aacggttttc gctggcgaat
     2941 ccaccgaaaa ccgttg
//
LOCUS       DASTEY010000140         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_514226, whole genome shotgun sequence.
ACCESSION   DASTEY010000140 DASTEY010000000
VERSION     DASTEY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514226"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1836)
                     /gene="ftsH"
                     /locus_tag="VFO72_02455"
     CDS             complement(46..1836)
                     /gene="ftsH"
                     /locus_tag="VFO72_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9478176.1"
                     /translation="MPTPTPRVSVRTRRVALACLAVVTFSLVIAASIVTYQRRQPPVQ
                     RINYSQLYSLAEAGGAVALQVEGETVTVTRTDGSIVEATVTGESTQHDVVQLFRKNNV
                     PVEFRPLQPGMWVSVLNYLLPVLTLGLIGVVAWRIYASMSGRGSFELTDHNGQQSVGF
                     DDVAGVDEAKAELTETIEFLRNPERFSRLGGRVPRGILLYGPPGTGKTLLARAAAKEA
                     GVAFLAASGSSFQEKFAGLGAARVRHLFAKGRKLSPCIIFIDEVDALGRQRGRGGDSA
                     SADQDQTLNQLLVEMDGFDQSSAVVVIASTNRPDILDKALTRPGRFDREIAVDLADVG
                     GREAILRVHARNLKLEDGLDLQWIARGTPGFSGAELANLMNEAAIAATRDEAPAVGRT
                     HVEFARDRILMGAERQGFMMDEDERYATAVHEAGHVAVGLAVENGDPIHKVSILPRGR
                     ALGVTQSLPERDRFMKKREYLEDQIALLLGGRAAEQVLLSTMTAGASNDIERAVDIAR
                     RMVSEFGMSRLGPIHLGQSDQPISQNLLDRIEEVTNELMSAQLERACAIVTAEKESIG
                     RLVELLMERDTLDAEEILSCFSNSPSVRAA"
     gene            2214..2366
                     /locus_tag="VFO72_02460"
     CDS             2214..2366
                     /locus_tag="VFO72_02460"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET9478177.1"
                     /translation="MLWTIFVILLVLWLLGVVSSYTIGGFIHLLLVLALVVLLINIIG
                     GRRTVV"
     gene            complement(2460..>2704)
                     /locus_tag="VFO72_02465"
     CDS             complement(2460..>2704)
                     /locus_tag="VFO72_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478178.1"
                     /translation="IFEEKESAEPYVLEALAREMTAANPDLKREFEETLQTDSDLAAS
                     PIERLRFFYKRSPYWDPQMNLYPVGRITSVVNLPLL"
BASE COUNT          609 a          831 c          707 g          557 t
ORIGIN      
        1 ctgacgcgcc ttcggcgcga acgcaaccct cacgcgccgt cggcgctatg cggcgcgcac
       61 gctggggctg ttactgaaac aactgagaat ctcttccgcg tccagcgtgt cgcgctccat
      121 caacaactca accaatcggc caattgactc tttttccgct gtcacgatcg cgcacgctcg
      181 ctcgagttgc gcactcatca actcgttcgt cacttcttcg attcggtcga gcaaattttg
      241 actgattggt tgatccgact gccctaaatg gatcggcccg agccggctca tgccaaactc
      301 actcaccatc cgccgtgcta tatccactgc acgctcgatg tcgttcgacg cacccgctgt
      361 catcgtcgat aacaacacct gctccgccgc acgaccacca agcaacaacg caatctggtc
      421 ctcgagatac tcccgcttct tcatgaaccg atcgcgctcc ggcaacgact gcgtcacacc
      481 aagcgcgcgt ccacgcggca ggatcgaaac cttgtgaatc gggtctccat tctccaccgc
      541 gaggccgacc gccacgtgcc ccgcctcgtg aaccgccgtc gcataacgct cgtcttcgtc
      601 catcatgaag ccctggcgtt cggcgcccat caaaatccgg tcacgagcaa actcgacgtg
      661 cgttcgtcca acggccggag cctcatcgcg ggttgccgca atcgcggctt cgttcatcaa
      721 attcgccaac tcggcacccg aaaagccagg cgtgccgcgc gcgatccact gaaggtcgag
      781 accgtcctcg agtttgagat tgcgtgcgtg aaccctgaga atcgcctcgc gtccgccaac
      841 atcagccagg tcgacagcaa tctcccgatc aaatcgcccg ggacgcgtca gtgccttgtc
      901 aagaatgtca ggccggttgg tcgaagcgat gacgaccacc gcgctggact gatcaaaccc
      961 atccatctcg accagcaact ggttcagcgt ctgatcctga tccgccgagg cagagtcgcc
     1021 gccgcgtcca cgctgccggc caagcgcgtc aacctcgtcg ataaagatga tgcacggcga
     1081 aagcttgcga cccttcgcaa acagatgccg cactcgcgcc gcgcccagtc cggcgaattt
     1141 ttcctgaaag ctcgacccgg atgctgcgag aaaagccaca cccgcttcct tggccgcagc
     1201 tcgcgccaac aatgtcttgc cagttcccgg aggaccgtaa agcagaattc cgcgcggaac
     1261 gcgtccaccc agacggctaa atcgctccgg attgcgcaaa aactcgattg tctccgtgag
     1321 ttcagccttg gcttcatcta caccggccac gtcgtcgaaa ccgacagact gctgaccgtt
     1381 gtgatccgtc aattcaaacg acccccggcc actcatgctc gcgtagatcc gccacgcgac
     1441 cacaccaatt aatccgagcg tcagcaccgg taacaggtaa ttcaaaacgc ttacccacat
     1501 tccgggctgc aacggccgga actccaccgg cacgttgttc ttgcgaaaca gttgcactac
     1561 atcgtgttgg gtggactcgc cggtaactgt cgcttcaaca atcgagccgt cggttctggt
     1621 cacggtcacc gtctcacctt caacttgcag ggcaacagcg ccgccggctt cagccagcga
     1681 atagagctgg gagtaattaa tgcgttggac aggcggttgc cggcgttgat aggtgacaat
     1741 tgaggcggcg attacgaggg agaacgttac gactgcaaga caagccaaag ccactcggcg
     1801 agtacggacc gatactcggg gcgtgggggt gggcatacaa aacctccaaa aaacgccgcc
     1861 cgttagtcat tcgggtggcg cgtgaaatta gcttcaaacc gcaaagaatc gggttaggaa
     1921 gagcttcgga acttcaatgg aaggacctgc gcgaactgag atgctcgtaa aacaccgatg
     1981 cgctcgccga agtgcgcacg caagcgtcac tttagcagcc aatatcgagt ttagtccagc
     2041 agaagatgaa aaacgggcac gaatcgggcc tgactgccac atttccatac gcccttaccc
     2101 ggatctgccg ttaaaaacgc caaatcggaa acggcatggg cgttgctcat ttcacttcgc
     2161 gattgctttt gtaccaacga aaagggcgtg gttgacaaaa ggaggagaat gaaatgcttt
     2221 ggacgatttt tgtaatcctg ttggtgttgt ggttgctcgg cgttgtcagt agctacacga
     2281 ttggtgggtt cattcatctg ttgctggtgt tggcgctggt agtgctgctt atcaacataa
     2341 tcggtggacg tcgtacagtc gtttaggcaa acaaaacaca ctgtttgccc tgctcgacgc
     2401 ccggcactta ccgcccgaca gtagtgttgg cgacgcggaa gcttcgggcg tccgaactat
     2461 cagagaagag gtagattaac cacacttgtg attcgcccta ctggataaag attcatttgt
     2521 ggatcccagt agggcgaacg tttgtaaaag aaccgcaagc gctcgatagg actagcagcc
     2581 aaatccgaat ccgtttgaag cgtctcttca aactcgcgct tgaggtccgg atttgcggcc
     2641 gtcatttcac gtgcgagcgc ttcgagcacg tagggttcgg cagactcttt ctcttcaaag
     2701 atcg
//
LOCUS       DASTEY010000141         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_85692, whole genome shotgun sequence.
ACCESSION   DASTEY010000141 DASTEY010000000
VERSION     DASTEY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85692"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFO72_02470"
     CDS             complement(<1..364)
                     /locus_tag="VFO72_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478179.1"
                     /translation="MRRTESEVGSRKLVFLNVPEGTFVLLPDEKVYANTSSDAPVVGS
                     AEDGELTPEKLLHDDIGSTSYQKLGTESVAGRNTNKYRVVVNKSNPANVSPIETLIWI
                     DEALGMPIRSETKSPDGTQ"
     gene            complement(598..1779)
                     /locus_tag="VFO72_02475"
     CDS             complement(598..1779)
                     /locus_tag="VFO72_02475"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET9478180.1"
                     /translation="MKNIYLAGAVRTPIGRFGGTLASWSAADLGVAVAKESLRRAGVA
                     VDQITDSIWGCARQAGGGPNVARQITWRAGVPETVPAATINQACGSGLKAIILAAQEI
                     MLGRSDAILAGGTESMSRVPYFAEGARWGMRMGNTQLVDGMYRDGFSDPLSGLLMGQT
                     AEKLARQYEISRQEQDEFALRSQQRAEAAIESGRFKDEVMTLEITGKKGESTSFDKDE
                     HCRAGTTLQDLAKLQPVFYKDGTVTAGNSSGITDGAAAVVVLSEDGLKRFGATAQARI
                     IDYEICGVAPDIMGIGPVPAVRALLQRQRLNLSEIDLIELNEAFAAQVIACDRELDFD
                     ADRLNVNGGSIALGHPIGCTGVRITTTLIHEMQKRQARLGLATLCVSGGLGLALLLER
                     A"
     gene            1834..>2435
                     /locus_tag="VFO72_02480"
     CDS             1834..>2435
                     /locus_tag="VFO72_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478181.1"
                     /translation="MLQRLTSPTLLEFAFQQIAALFRAHAEWLYVANDGTSQSLRRDE
                     LDVAIVHNRLVLSCWTEKGNRLWRVLAWNWNGQALELETSRRMGARRELIQLIPRTTA
                     KHVAATIRAARQLRCERLAQLAATLEPQTAIERLALSRGTRPGLPGRHAQILLRRKRE
                     RIAVTGPVVASQPGVVDSFLSSVLLWFRRTSDRIKPPYVE"
BASE COUNT          464 a          685 c          720 g          566 t
ORIGIN      
        1 cctgcgtgcc atcgggtgat ttggtctcgg atctgatggg catgccgaga gcttcatcaa
       61 tccagatcaa tgtctcaatt gggctgacgt tagccgggtt cgatttgtta acaacgacgc
      121 gatacttatt tgtgttgcgc cctgctaccg actcggtgcc gagcttttga tagctggtgc
      181 tcccgatgtc gtcgtgaagc agtttttccg gtgtgagctc cccgtcctct gctgagccaa
      241 cgactggtgc atcgctcgag gtgtttgcgt agactttttc gtcaggcaat aaaacgaacg
      301 tgccctcagg aacgttgagg aagaccagct ttctcgatcc gacctcggat tcagtgcgac
      361 gcatctcgcc gtctttagca gtcgagcttt ttgcgacggt ggttttcccg tcggctgtaa
      421 cgctggtgat agtgcgtgtg gctcgataac gctccggttc ctttgtttga aagggaggcg
      481 tagaagcgac agtatccgcg ggagcgctgt tcgttgcagg tgagttgttt tgagaactgc
      541 acgaggcaaa aacgagaagg gagcaacaga gtagcgcccg ctttcgtttc agctggttca
      601 tgcgcgttcc agcaggagtg cgagtccgag accgccgctg acgcagagtg ttgcgagtcc
      661 gagtcgcgcc tggcgctttt gcatttcgtg tatcagcgtg gttgtgatcc gaacgccagt
      721 gcaaccgatt ggatggccca aagcgatcga gccaccgtta acgttcaagc gatcggcatc
      781 gaaatcgagt tctcgatcgc acgcgatcac ctgcgccgcg aaggcttcgt tcagttcgat
      841 taaatcaatc tcggaaagat taagacgttg tcgctgcaac aacgcgcgca cagcgggcac
      901 tggcccaatg cccatgatat ccggtgcgac gccgcagatc tcataatcga taattcgcgc
      961 ttgcgccgtg gcgccaaagc gtttcagtcc gtcttcactc aagacaacca cagctgcggc
     1021 gccatcggtg ataccggatg agttgcccgc agtcactgta ccgtctttgt agaagactgg
     1081 ttggagtttc gccagatcct gaagtgtcgt gccggcgcgg caatgttcgt ccttgtcaaa
     1141 gctggttgat tcgcccttct tgccggtgat ctcgagagtc attacttcgt ctttgaaccg
     1201 gccggattcg attgccgctt ctgctcgctg ctgcgaccgc agtgcgaatt cgtcctgttc
     1261 ctgacgcgag atctcatact gtcgcgcgag cttctccgcg gtctggccca tcaacagtcc
     1321 cgaaagggga tcgctgaagc cgtcgcgata catgccgtcc acgagttgcg tgttgcccat
     1381 gcgcatgccc cagcgtgccc cttcggcgaa ataaggcacg cgcgacatgc tttccgtgcc
     1441 gcccgcgagt atggcgtcgc tgcgaccgag catgatttcc tgcgcggcga gaatgatggc
     1501 ttttagtccg gagccgcacg cctggttgat cgtggcggca gggactgttt ccggcacgcc
     1561 ggcgcgccat gtgatctgtc gcgctacgtt tggtccaccg ccggcctggc gcgcgcaacc
     1621 ccagatcgag tcggtgattt gatcgactgc cacgcctgct cggcgcagac tttcctttgc
     1681 taccgcaacg ccgagatcgg ctgcactcca cgatgcgagg gtgccgccaa atctgccgat
     1741 tggtgttcgt actgctcccg cgagataaat gttcttcatg atttagttga ccaaaaatgt
     1801 agcacagggt agaagtaacg acgaaacctc acgatgcttc agcgcctcac atctccgacg
     1861 ctattagagt ttgcgttcca gcaaatcgcg gcgctgtttc gcgcgcatgc tgagtggctg
     1921 tatgtcgcga atgatggaac gtcgcaatcg ttacgacgag acgaactcga cgttgcgatc
     1981 gttcataata gacttgtcct ctcgtgctgg actgaaaagg gaaaccggct ctggcgtgtc
     2041 cttgcgtgga actggaatgg acaggcgcta gagttggaga cttcgcgacg gatgggcgcc
     2101 aggcgcgagc tgattcaact gattccacgc actaccgcga aacacgttgc cgcgacgatc
     2161 cgtgccgcgc gccaactccg atgcgaacgt ctggcccaac tggcggcgac tctggagcct
     2221 cagacggcga tcgagcgatt ggcgttgagt cgcggaacga gaccagggct gcccggccgt
     2281 cacgctcaga ttctgctgcg aaggaaaaga gaaagaattg cggtcactgg tccagtcgtg
     2341 gcgagccagc ccggcgtggt tgactcgttc ttatcgtcgg tgcttttgtg gttccggcgt
     2401 acgtccgatc gcatcaagcc gccgtatgtc gagca
//
LOCUS       DASTEY010000142         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_349157, whole genome shotgun sequence.
ACCESSION   DASTEY010000142 DASTEY010000000
VERSION     DASTEY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349157"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VFO72_02485"
     CDS             <1..1350
                     /locus_tag="VFO72_02485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9478182.1"
                     /translation="RKRLLLLLCALILTFCVRGLTMQFMRAHLHDPAWFQVGSYAKFD
                     RQARDILDGKQRLFWIDDPTRTDLAQYPPAFPAAVALIYKLGGNRSAYAVQSVQWIAD
                     SVISFVLIAGVAVTAFGWRAGIAASFLFALSPLLAMYGAYPSADAPAMWFILRGCWLL
                     LLAALRNNVWLALGAGVLLGIACWFRVNPLYLCVGWAVAMFLFTRTEWRRRSMLAGAV
                     LIGTVLIIAPIVVRNYVVFPDFTPTGGTIGTNLWEGLGETEFGRQHGFFFGDDKMIEH
                     ERVKLGLPPDFPIDAQWPDGIRRDHERTRESLAFIKQHPVWYAGVMLRRMWWMLKVAG
                     KPAPYAGSSGINVTSRKCLPPERQGGVIAAGVNLLGMVQSVARYLLLPLAVFGLFRAL
                     RQDWRISGVLLMTVLYYLVPGTAGHTEIRYVLPMHALLIVFAGAGLIKHGDRNKRGV"
     gene            1340..2125
                     /locus_tag="VFO72_02490"
     CDS             1340..2125
                     /locus_tag="VFO72_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9478183.1"
                     /translation="MAFNSETNTIQGALPREMMEHTYPILFRVEQSHWWYIGRRRIIA
                     RFVEDICRRVTDRQPRILDVGCGTGANLLLLSKYGDAEGVDVSEDALAFCRERGLDHV
                     KQGAGEELPYEDATFDLVTAFDVVEHMDDDLAGLKEMYRVLRPGGYALLFVPTFMFLW
                     GLQDEVSNHRRRYRLPELRRVLEQAGFEIERSTYANITFFLPILLVRKLMRLTRIKTS
                     TENTINVSALNGVFGALFGFESAILRYINLPFGVSGLCVAKKP"
BASE COUNT          429 a          606 c          708 g          564 t
ORIGIN      
        1 cgcaaacgac tgctcctgtt gctctgcgct ttgatcctca cgttctgcgt acgaggcctc
       61 acaatgcagt tcatgcgtgc tcaccttcac gatcctgcgt ggtttcaggt gggctcttac
      121 gccaagttcg atcgccaggc acgcgatatt ctggacggca aacaaagact gttttggatc
      181 gacgatccaa cgcgcaccga tctcgcgcag tatccgcccg cattcccggc agcggtggcg
      241 ctgatttaca aactaggcgg caaccgttcg gcatacgccg ttcaaagcgt gcaatggatt
      301 gcggattcgg ttatctcgtt cgtgctgata gctggcgttg cggttacggc gttcggttgg
      361 cgcgctggaa tcgcggcgag ttttctgttt gcactgtcgc cgctacttgc gatgtacggc
      421 gcatatccat ccgcggatgc gccggcgatg tggttcatac ttcgcggctg ctggttgttg
      481 ctgctggctg cgctgcgtaa caacgtatgg ctcgcgcttg gcgcgggcgt gttgctcgga
      541 atcgcgtgct ggtttcgtgt gaacccgttg tacttatgcg tcggttgggc ggtggccatg
      601 tttctgttta cgcgaacaga atggcgccgc cgatcaatgc tcgcgggcgc ggtgctgatt
      661 ggaactgttc ttatcatcgc gccaatcgtc gtgcgcaact acgtagtttt tcctgacttc
      721 acgccaaccg gcggaacaat cggcacgaac ctttgggagg ggctcggcga gactgaattc
      781 ggacgccaac acgggttttt tttcggcgac gacaagatga tcgaacacga gcgagtgaag
      841 cttggtttac cgcctgactt tccgatcgat gcacagtggc ccgacggcat tcgccgcgat
      901 cacgagcgca cgcgcgagtc gctggcattc attaagcagc atccggtgtg gtatgccggt
      961 gtgatgttgc ggcggatgtg gtggatgctg aaagtggcgg gcaagccggc gccttatgcg
     1021 ggctcgagcg gcatcaacgt gacgagtagg aaatgtctgc cgcccgagcg tcaaggcggt
     1081 gtgattgcgg ctggtgtcaa cctgctcggg atggttcaaa gcgtggcgcg gtatcttctt
     1141 ctgccgttgg cggtgtttgg gctttttcga gcattacggc aagattggcg cattagcggt
     1201 gtattgttga tgacggttct ttactatctc gttcctggaa cggccgggca cacggagatt
     1261 cgctacgtgc tgccgatgca tgcgttgttg atcgtctttg cgggagcggg tttaataaag
     1321 cacggagacc gtaacaagcg tggcgtttaa ctccgaaact aacaccatcc aaggcgcact
     1381 gccgcgcgag atgatggagc atacctaccc gattctcttt cgggtggagc aatcgcattg
     1441 gtggtacatc gggcggcgcc gcatcatcgc ccgttttgtc gaggacatct gtcgtcgcgt
     1501 caccgatcgg cagccgcgca ttttagatgt tggttgcggc accggggcaa acctgctgct
     1561 gctttcgaaa tatggcgacg ctgagggagt tgacgtttca gaagacgcgc ttgctttttg
     1621 tcgcgaacgt ggtttggacc acgtcaagca gggcgcgggc gaagagttgc cctacgagga
     1681 tgctacgttc gatctcgtga ccgcattcga cgtcgtggaa cacatggacg acgatctcgc
     1741 cggcttgaag gagatgtatc gagtacttcg tccgggtggt tatgcccttt tgtttgtgcc
     1801 gacgttcatg ttcttgtggg gcttgcaaga cgaggtgagc aatcatcgcc ggcgttatcg
     1861 cttgcctgag ttgcgacgcg tgctcgagca ggcgggcttt gagatcgagc gttcaactta
     1921 tgcgaacatc actttcttcc tacctatttt gctcgtgcga aaactgatgc ggctgacgag
     1981 aataaaaacc tcgacagaaa acaccatcaa cgtgtccgcg ctgaacggag ttttcggagc
     2041 gctgtttggc ttcgagagcg ccatcctgcg ttacatcaac ctgccttttg gcgtgtccgg
     2101 tttatgcgtc gcgaagaagc cgtagcagca gcgggccggt gacggccacg gatgagcacg
     2161 gactcaagca tcggtcatag cgtgcggtct aatgtatcat gccgcaaagc agcatgttgc
     2221 acgaccaatc tataatctgc tttggcggcg aagactggtg gtatcatcac ccgcactcca
     2281 aagcacacct gatgcgaaag ttcgcgc
//
LOCUS       DASTEY010000143         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_942566, whole genome shotgun sequence.
ACCESSION   DASTEY010000143 DASTEY010000000
VERSION     DASTEY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942566"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VFO72_02495"
     CDS             complement(<1..1001)
                     /locus_tag="VFO72_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9478184.1"
                     /translation="MASNALKIAVVGGGLAGLAAAMKIAEGGHAVDLFSVVPVKRSHS
                     VCAQGGINGAVNTKGEGDSTWEHFDDTVYGGDFLANQPPVKAMCEMAPSIIYLFDRMG
                     VPFSRTKEGLLDFRRFGGTKHHRTAFAGASTGQQLLYALDEQVRRYEVKGNVRKFEHW
                     EMMSLVLDDHQVCRGLIAMDLQTLELKAFPADAVIIATGGPGLIFGKSTNSMVCTGSA
                     VSACYQQGAKYANGEFIQVHPTSIPGEDKLRLMSESARGEGGRVWVPRKKGDTRSPSS
                     IPENERWYFLEEKYPAYGNLVPRDIATREIFQVCLEEMGIGGQNQVYLDLTHIPADVL
                     "
     gene            complement(1001..1675)
                     /locus_tag="VFO72_02500"
     CDS             complement(1001..1675)
                     /locus_tag="VFO72_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HET9478185.1"
                     /translation="MAIRLSRTFILRKLHQFSGIFPLGIFLLEHFYTNSKALTGPGDF
                     NSAVRDLQSIPYILFVEIGGIFIPLIYHAVYGLVITWEARPNNLNYPYPRNWFYLIQR
                     ITGIVLFFFILFHVLNFRFGMIPGLNTISVAHEPDKAFDIVAGEFRMVPIFLIYMVGI
                     TATVWHFANGIWLFLVDWGITIGERAQRLTGYACIAFGVVLLLVGVNAAVAFIRPGGL
                     LGGIIY"
     gene            1787..>2189
                     /locus_tag="VFO72_02505"
     CDS             1787..>2189
                     /locus_tag="VFO72_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9478186.1"
                     /translation="MQNKGLCEQSLTQLAELIRKREVSPVEVVEAHLARISNLNPALN
                     AIVTLASNVTERSEGVLRGLPITVKDTIETAGIRTASGSRIRADYVPARDAPAVARLK
                     AAGAIILGKTNAAEMAMDYTADNPVFGRTVHP"
BASE COUNT          540 a          581 c          629 g          439 t
ORIGIN      
        1 agaacgtcag cgggaatgtg cgtcaaatcg aggtagacct ggttttgacc gccaataccc
       61 atttcttcca gacacacctg gaagatctcg cgcgtggcga tgtcgcgcgg cacgaggttg
      121 ccgtacgccg gatatttctc ctcgaggaag taccagcgtt cgttctccgg aatggacgaa
      181 ggcgacctcg tgtcaccttt ctttcgcggc acccaaacac gcccaccttc gccacgcgct
      241 gactccgaca tcaggcgcaa cttgtcttcg cccggtatcg acgttgggtg aacctgaata
      301 aactcgccgt tcgcgtactt cgcgccctgt tgatagcagg cggagactgc gctaccggtg
      361 cagaccatgg aattggtcga cttgccgaaa atgagacctg gtccaccggt ggcgatgatc
      421 actgcgtcgg caggaaaggc tttcagttcg agggtctgaa gatccatcgc aatgagaccg
      481 cgacagacct gatgatcgtc gagcaccagc gacatcattt cccagtgctc aaacttccgc
      541 acgttgcctt tgacttcata acgccgcact tgttcgtcga gcgcgtagag cagttgttgt
      601 ccggttgagg cgccggcaaa cgcggtgcgg tgatgcttgg tgccgccgaa gcgacggaag
      661 tcaagtaatc cttctttggt gcgtgagaag gggacaccca tgcgatcgaa caagtagatg
      721 atcgacggcg ccatctcaca catcgccttc acgggaggct gattggcgag gaagtcgccg
      781 ccgtagacgg tgtcgtcgaa atgttcccac gtcgaatcgc cttcgccttt agtattgact
      841 gcgccgttga tgccgccctg ggcgcaaaca gagtgcgaac gtttcacggg cacgactgaa
      901 aacagatcga ccgcgtgacc gccttccgca atcttcatcg cggcagcaag accagcgagc
      961 ccacccccga caacggcgat cttcagagca ttacttgcca ttaataaatg atgcctccta
     1021 acagtccgcc cggacgtatg aacgcgacgg cggcgttaac gcccaccagc agcagcacaa
     1081 cgccgaaggc gatacaagca taaccagtca ggcgttgtgc gcgctctccg atggtgatac
     1141 cccagtcgac gagaaacagc cagatgccat tcgcgaagtg ccagacggta gcggtgatgc
     1201 cgaccatgta gatgagaaag atcggcacca tgcgaaactc accggcaacg atgtcgaaag
     1261 ccttgtccgg ctcatgggcc accgagatag tgttaagtcc cggaatcatc ccgaaccgga
     1321 aattgagcac gtgaaacagg ataaagaaaa acagaacgat tccggttatt cgttgaataa
     1381 ggtaaaacca gttgcgcggg tagggataat tcaaattgtt tggccgcgcc tcccacgtga
     1441 tcaccaggcc gtagacggcg tgatagatga gcggtataaa gatgccgccg atctcgacaa
     1501 atagaatgta gggaatcgac tgtagatcgc ggacggcgct gttgaaatcg cctgggccgg
     1561 tcaaagcctt ggagttggtg tagaaatgtt ctagaagaaa aattccaaga gggaaaatac
     1621 cggaaaactg atgcagcttg cgcaggatga aggtcctgct caaacggata gccaagaatg
     1681 cactcctttg ccgttatgct tgaacatcga cgccagtcga tttgaaccag gcaggttgta
     1741 aacgagtttg gacagagcaa ttgaattata ttgggaagtt gacgaaatgc aaaacaaggg
     1801 tctctgcgaa cagtcgctga cacaacttgc cgaactgatt cggaagaggg aagtgtcgcc
     1861 ggtggaagtc gttgaggcgc atctggcacg gatttcaaat ttaaacccgg cgttgaatgc
     1921 gattgtgacg ctggcgtcga acgtgaccga aagatcggaa ggcgttttac gcggactgcc
     1981 aatcacggtc aaggacacga tcgagaccgc tggtattcga acggcaagtg gatcacggat
     2041 cagagcggat tacgtgcccg cgcgtgatgc tcccgcggtt gcgcgtttga aagctgcggg
     2101 tgcgatcatc cttggcaaga cgaacgccgc cgaaatggcg atggactaca cggcggacaa
     2161 tccggttttt ggacgtacgg tccatccgc
//
LOCUS       DASTEY010000144         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_415380, whole genome shotgun sequence.
ACCESSION   DASTEY010000144 DASTEY010000000
VERSION     DASTEY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415380"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /gene="asnB"
                     /locus_tag="VFO72_02510"
     CDS             complement(<1..1701)
                     /gene="asnB"
                     /locus_tag="VFO72_02510"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9478187.1"
                     /translation="MCGINGIAYSSRSGRMVSRAHLERMRDVITHRGPDDEGIFIDGN
                     VGLGHRRLSIVDVAAGHQPMTNEDSTLHITYNGEIYNHSEYRESLEARGHVYRTHCDT
                     ETILHLYEDHGPSCVDYLRGMFAFAIWDQRKRELFIARDRLGVKPLYYVRTDDGSLYF
                     GSEIKTLLEAGAVKPEINYAVLSDYLANHAPSGEETLFRGVKRLMPGHHLLWRDGEVS
                     IQRYWDVDFSKDASSTRDEKAYIQDWTELFRESVRLRLMADVPLGMFLSGGIDSSAIA
                     AMMSGMVSEPIKTFSVAFAEREANELEYARLVAARYKTNHHEIVVTPEQFFAALPQLV
                     WHEDEPLAHPSSVALYFVSKLASEHVKVVLTGEGSDELLAGYGRYHRTTLNLALGRRY
                     HQIAPGFLRDAIRRQIEKLEPVSRVRHKLMRTFLVLSPELESIYFDNFAVFPAPMQQH
                     MFTKRTSDQIHEIDPYVELRKLLERTKGLSLLDGLLYADIKTYLHELLMKQDQMSMAT
                     SVESRVPFLDHKLVEFSAKMPENMKLRGATTKYVLRESMKGVLPEKILSRSKMGFPVP
                     I"
     gene            complement(1688..3901)
                     /locus_tag="VFO72_02515"
     CDS             complement(1688..3901)
                     /locus_tag="VFO72_02515"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET9478188.1"
                     /translation="MNLQKIRNASLRELRVRAAQRVAAFRERRGWSTLARLPSDGTLR
                     SLLNIGNVDYLEYFRIRSEPRFFADPRAAAMEFKSRWPHAAQQLIEQADRITLGRFDL
                     LGLRELSFGHPIDWHFEPVSGKRIPILHWSKLDYLDAELAGDKKVIWELNRHQYFVRL
                     GQAYCLTGDERYARVFASHLEGWLDANPPKLGVNWASSLEVAFRSISWLWAFYFFKSS
                     PSLSLKTFKRAVKYLYLSARHLESYLSTYFSPNTHLTGEALGLFYLGTLLPEFKEAKR
                     WQATGSRILIDALPVHVQKDGVYFEQSSYYHRYTTDFYIHFLLLARANGFALPNDVEK
                     SLALLLDHLMYITRPDGTTPLFGDDDGGRLMPLDQRPANDFRATLATGSILFNRGDLK
                     FVADDATEELVWLVGVEGLKQFDSIPAQPPAKLSQGFLKGGYFVMRDGWSHDSNYLLF
                     DCGPHGALNCGHAHADALSIDLAVNGHTMLVDPGTYTYTGSKELRDWFRSSQAHNTVT
                     IDGESSSIPNEAFSWKTKAACTLKNWITTDRLDFVSGTHDGFQRLADPVSLERSILFL
                     KHDYFVIRDLFKFTSEHRADVWFHFDPSSIPSLEAGVIRETHAGFSIQSFGNGHWIEE
                     EASVSHCYAQKERAPAYAFSASFRGEGEIVSFLLPWQHGIEWRVTEIEVHGNPAFVVS
                     GAKTLDLVMIRATDWLWVRFLGEKAQEIVTLPNERTLTSGEIEDLYSVVKQNVRH"
     gene            3982..>4457
                     /locus_tag="VFO72_02520"
     CDS             3982..>4457
                     /locus_tag="VFO72_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9478189.1"
                     /translation="MKFSVIISTHNRADSLRETLAGQARLQTRAEWELIVVDNNSTDH
                     TRAVVEEAAKNFPVELRYLFEAEQGKFAALNQAILASRGEIIATTDDDAFAEPDWLDR
                     AAEGLERFNCDFVGGKVLPVWGGPRPQWLPDRPGMHWAVIALLDLGEEPVEFGCKGA"
BASE COUNT          970 a         1219 c         1292 g          976 t
ORIGIN      
        1 gatcggcacc ggaaatccca ttttgctgcg cgaaagaatt ttctccggca agacgccttt
       61 catactctcg cgcagcacgt atttcgtcgt cgcgccgcgc agcttcatgt tctccggcat
      121 ctttgcactg aactcgacca gcttgtgatc gaggaaaggc acgcgactct ctactgaagt
      181 cgccatgctc atctggtcct gcttcatcaa cagttcgtgg agataggtct tgatgtcggc
      241 gtacaagaga ccatcgagca gcgagagtcc tttggtgcgc tcgagcagtt tgcgcagttc
      301 cacgtaagga tcgatttcgt ggatttgatc gctcgtgcgc ttcgtgaaca tgtgttgctg
      361 catcggcgcc ggaaatactg cgaagttgtc gaagtaaatg ctttccaatt cgggcgacaa
      421 cacgaggaac gtgcgcatta acttgtgtcg cacgcgcgaa acgggttcga gtttctcgat
      481 ctgccgtcga atggcatctc gcagaaaccc gggcgcgatc tggtgatagc gccgccccag
      541 cgcgagattc aaagtcgtac ggtgataacg gccatatcca gcgagcagtt cgtcgcttcc
      601 ctcacccgtg agcacgacct tcacatgctc ggaagcaagt tttgaaacga agtagagcgc
      661 cacactcgac ggatgggcca atggctcgtc ttcgtgccag accagttgcg gcagcgcggc
      721 aaagaactgt tcgggcgtga caacgatctc gtgatggttt gtcttgtatc ttgcagcgac
      781 tagccgcgca tactccaact cgtttgcttc acgctccgcg aacgcgacgg aaaacgtttt
      841 gattggctcg ctgaccatgc cgctcatcat cgccgcaatt gcggaagaat cgatgccgcc
      901 tgaaagaaac atgccgagtg gcacgtcggc catcaatcgc aggcgaaccg attcacgaaa
      961 gagttcggtc cagtcctgga tgtaagcttt ttcgtcgcgc gtgctgctgg cgtcttttga
     1021 gaagtcaacg tcccaataac gttgaatgct aacttcgccg tcgcgccaga gcagatgatg
     1081 tcccggcatg aggcgcttga cgccgcgaaa gagtgtttct tcgccggatg gcgcgtggtt
     1141 cgcgaggtag tcggacagaa cggcgtagtt gatctccggc ttgacggcgc ccgcctcgag
     1201 cagcgttttg atctctgaac cgaagtagag cgacccgtcg tcagtgcgaa catagtagag
     1261 cggcttcacg ccgagacgat cgcgcgcgat gaacaactcg cgtttacgct ggtcccagat
     1321 cgcaaaagca aacatgccgc gcaagtaatc gacacaactc gggccgtgat cttcgtaaag
     1381 gtggagaatc gtttccgtgt cgcaatgcgt gcggtagacg tgcccgcgcg cttcgagtga
     1441 ctcgcgatat tctgagtggt tgtagatctc gccgttgtag gtgatgtgca gcgttgagtc
     1501 ttcgttggtc atcggttgat gaccggcggc aacgtcgacg atgctgaggc gacgatgacc
     1561 gagaccgacg ttgccatcga tgaagatgcc ttcgtcgtcg ggaccacgat gagtgatcac
     1621 gtcgcgcatt ctttcgaggt gcgcgcgcga aaccattcgt cccgaacggc tggagtaggc
     1681 tattccgtta atgccgcaca ttctgtttca ctacggagta aaggtcttct atttcgccgg
     1741 aagtcaaagt tctctcattc ggcaacgtta cgatctcttg cgccttctct cccaggaacc
     1801 tgacccaaag ccagtccgtg gcgcgaatca taacaagatc cagagtcttt gccccagaga
     1861 ccacaaaagc cgggttgccg tgcacctcga tttccgtcac tcgccactcg attccgtgct
     1921 gccacggcag caggaagctc acgatttctc cttcgccgcg gaagctggcg gagaaggcgt
     1981 aggccggggc tcgttccttt tgagcataac agtgcgaaac cgaagcttct tcctctatcc
     2041 agtggccgtt accgaagctc tgaatactaa aaccagcgtg tgtttcgcgt atcacgcccg
     2101 cttcgaggct cggtattgag gagggatcga agtgaaacca cacgtctgca cgatgttcgg
     2161 acgtgaattt gaacaaatct cggatgacga aataatcatg tttgagaaac aggatgcttc
     2221 gttcgaggct aactggatcg gcgagacgtt ggaagccgtc gtgcgtgccg gagacaaagt
     2281 cgaggcggtc ggtggtgatc cagtttttga gtgtgcacgc ggcttttgtt ttccaggaga
     2341 acgcttcgtt tggaattgaa gatgattcgc cgtcgatagt gaccgtgttg tgtgcctgcg
     2401 aactgcgaaa ccagtcgcgc agttctttcg agccggtgta ggtgtaagtg ccgggatcga
     2461 ccagcatcgt gtggccgttc acggcaagat cgatcgacaa ggcgtcggcg tgtgcgtgtc
     2521 cgcagttgag cgcgccgtgt ggaccgcagt cgaagagcag gtagttggag tcatgactcc
     2581 agccgtcacg cattacgaag tagccgcctt ttaaaaaacc ttgcgacaat ttcgccggag
     2641 gctgagctgg tatcgagtca aactgtttta gaccctcgac acccaccaac cacaccaact
     2701 cctctgtggc atcgtccgcc acgaatttta gatcgccacg attaaacagg atcgagccgg
     2761 ttgcgagagt ggcgcggaaa tcattggccg ggcgctggtc caatggcatc aacctcccgc
     2821 cgtcgtcgtc tccgaacagc ggagtggtgc cgtcagggcg ggtgatgtac atcaagtggt
     2881 ccaaaagcaa cgcgagcgat ttctcaacgt catttggcaa tgcgaagcca ttcgcgcggg
     2941 cgagcagcag gaagtgaatg taaaaatcgg tcgtgtagcg atggtagtaa ctcgactgct
     3001 cgaagtagac gccgtctttc tgcacgtgca ctggaagcgc atcgatcaga atgcgactac
     3061 cggttgcctg ccagcgtttc gcctctttga attccggcag aagcgtaccg agataaaaca
     3121 ggccgagcgc ctcgcccgtg agatgtgtgt tggggctgaa gtacgtggat aagtacgact
     3181 cgagatggcg ggcgctcaga tacaggtact tcactgcgcg cttaaaggtc ttcaatgaga
     3241 gtgaaggcga cgacttgaaa aagtagaacg cccacaacca cgagatcgat ctaaacgcaa
     3301 cttcgagact gctggcccag ttaacgccga gcttcggcgg gtttgcatcc agccaacctt
     3361 ccaaatgact tgcgaacacg cgcgcgtatc gctcgtcgcc ggttaagcag tacgcctgac
     3421 ccagcctgac gaagtactgg tgccggttga gttcccaaat aacctttttg tcgcccgcca
     3481 gctcggcgtc gaggtagtcg agcttgctcc aatgcaaaat cgggatgcgc ttgcctgaga
     3541 cgggctcgaa gtgccagtcg atcggatgac caaaactcaa ctctcgcagg ccgagaaggt
     3601 caaaccggcc caaagtgatg cgatccgctt gttcgatgag ctgttgcgca gcgtgtggcc
     3661 agcgcgattt gaactccatc gcggctgctc gcgggtcggc aaagaaacgc ggttccgacc
     3721 gtattcggaa atattcgagg tagtcgacat ttcctatatt gagtaacgag cggagtgtgc
     3781 cgtcggaagg gagccttgca agcgtggacc aaccgcgccg ctctcggaac gctgcgacgc
     3841 gttgagccgc gcgcacgcgc aactcccgca aactggcatt cctgatcttt tggagattca
     3901 tcaaggcgcc gcgccagagt gtataacgtt tttctccctt tcttgcttga tagcaccgat
     3961 taaggaagaa tacagggttc catgaagttc tcggtaatta tctcgacgca caatcgcgct
     4021 gacagcctgc gcgaaacact ggccggccag gcgaggctcc aaacccgggc cgaatgggaa
     4081 ctgatcgttg tcgacaacaa ctcgaccgat cacacgcgcg cagtcgttga agaggcagcg
     4141 aaaaatttcc cggtcgagct tcggtatctt ttcgaagccg agcagggaaa gtttgccgcg
     4201 ctcaaccagg ctattctcgc ctctcgcggc gagatcatag cgacgactga cgacgacgct
     4261 tttgctgaac ccgattggct cgaccgcgcc gccgaaggtc tggagcgttt caactgcgac
     4321 ttcgtcggcg gaaaggtact tccggtctgg ggcggaccgc gtccgcaatg gctgccggac
     4381 agaccgggaa tgcactgggc ggtcatcgcg ttgctcgatc tcggtgaaga gccggtcgag
     4441 tttggctgca aaggcgc
//
LOCUS       DASTEY010000145         5143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_580037, whole genome shotgun sequence.
ACCESSION   DASTEY010000145 DASTEY010000000
VERSION     DASTEY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580037"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /gene="carB"
                     /locus_tag="VFO72_02525"
     CDS             complement(<1..1793)
                     /gene="carB"
                     /locus_tag="VFO72_02525"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9478190.1"
                     /translation="MPKRTDIHKILIIGSGPIVIGQACEFDYSGTQACKALRQEGYEI
                     VLVNSNPATIMTDPETADVTYIEPLNVASVTEIIRRERPDALLPTVGGQTALNLAIAL
                     SDAGVLDEYGVEMIGANRDAIKVAENRLLFKQAMDEAGLEMPRGGFATSWDEAREIVA
                     LTNYPAIIRPSFTLGGTGGGTAYNPEEFEEIVRGGLAASPIHEVLIEESILGWKEFEL
                     EVMRDLADNVVIICSIENFDPMGVHTGDSITVAPAQTLTDREYQLMRDMAIRVIRKVG
                     VETGGSNIQFGLNPENGDIRIIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRKTPASFEPTIDYVVAKIPKWDFEKFREAEDVLGTQMKSVGEVMAIGRTFKE
                     ALFKGLRSLEAVKPLRLEDISDDELQRKLARPNSHRFSYISYALQHEWPIAEIYRLSR
                     IDPWFLSQLKEVMDIQREIEGRKLEEISPDDLRAFKEVALSDRRLAYLTERSEDEVRA
                     RRKELGIVPVYKRVDTCGAEFESYTPYLYSTYETEDESAPTDRRKIMILGSGPNRIGQ
                     GIEFDYCCCHASFALHEAGFETIMVNCNPETV"
     gene            complement(1796..2374)
                     /locus_tag="VFO72_02530"
     CDS             complement(1796..2374)
                     /locus_tag="VFO72_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478191.1"
                     /translation="MAAARIPRRYQKCSFVNFISDPGTSQDHALRHAHKFANEYPAVD
                     RGLLFMGPAGVGKTHLAVSIIRDLIDKGFAGLFYEFGSLLKEIQDSYNPISKSSELKV
                     LAPVYQADVLVLDELGATVPTEWVRDTMYQIINKRYNDKKLTIFTTNYLDKSNELEER
                     ITYRLRSRLYEMCTNVVIDGEDYRRRRRPDIS"
     gene            2645..3064
                     /locus_tag="VFO72_02535"
     CDS             2645..3064
                     /locus_tag="VFO72_02535"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HET9478192.1"
                     /translation="MTNARAERHPIRVFLLDDDKRRHRWFSDRFKGDFIDIAQDVAEA
                     QRLLSASSYDAIFLDHDLHPEHYNTDSTDDERTGYAIAAWLCANPELQRASTILVHTR
                     NADGAMRMVGELRRAGRSADYVPFPMLAERIKTYWQR"
     gene            3579..3968
                     /locus_tag="VFO72_02540"
     CDS             3579..3968
                     /locus_tag="VFO72_02540"
                     /inference="COORDINATES: protein motif:HMM:NF023374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HET9478193.1"
                     /translation="MSIVANRANGSQRRTASRAGDSALARAFESRTSDIQVKGAGTVI
                     RVLPDDLKGSRHQRFIVQLASGQTLLITHNLDLAPRIDRLKVGDSVSFYGEYVWNEKG
                     GVIHWTHHDPQGRHVAGWLKHNGKTFQ"
     gene            complement(4024..>5143)
                     /locus_tag="VFO72_02545"
     CDS             complement(4024..>5143)
                     /locus_tag="VFO72_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET9478194.1"
                     /translation="KISDVKVGRNPVSMTVSKLSKAIYVTSETEGVVTVIDEQSQQVL
                     AEIKAKPGLRSIRFSPDGRYGFVLNPKENTVTIFDAASNRILHDAKVGNAPDQIIFSD
                     TFAFVRSLKTESISMLRLATINEDVIVTEFPGGQAPFEKASRTVQAEGMVLAPEGNAV
                     IVANPGDKTLYYYSEGMAAPMGNFQNYKREPLSVLIVDRSLRETDPGIYSATIKFPAS
                     GDYDIAFLNDSPRVSHCFEATAEPNPLLKEEQKVALRIEHQTKERRLPVGEDFPLRFQ
                     LFDTATGKPKADLTDVHVLTFLSPGIWQRRDIAKSLGNGVYEITVKAPQPGLYMVFVE
                     SASMRVEFRDLPYLTLQALPAKLAPPAASGQASTVAKP"
BASE COUNT         1172 a         1432 c         1348 g         1191 t
ORIGIN      
        1 acagtttcgg gattacagtt gaccatgatc gtttcaaatc ccgcttcgtg cagcgcaaac
       61 gacgcgtggc aacagcagta gtcaaactca attccctgcc cgatgcggtt tggaccagat
      121 ccgagaatca tgatcttgcg ccggtctgtc ggcgccgact catcttcggt ttcgtaagtc
      181 gaatagaggt aaggcgtgta tgattcaaac tcggcgccgc acgtgtcgac gcgtttataa
      241 accggcacga tgcccagctc tttgcgccgc gcgcgtactt catcttccga acgttcagtc
      301 aaatacgcca atcgccgatc cgacaaagcc acttccttga aagcacgcaa atcgtccggc
      361 gagatctctt cgagtttcct gccctcgatc tcgcgttgga tatccatcac ctctttcaac
      421 tggctgagaa accacggatc gattcgcgag agacggtaga tctcagcgat gggccactcg
      481 tgctggagcg cgtaagagat gtaagagaaa cgatgtgagt ttggccgcgc cagtttgcgt
      541 tgcaactcgt cgtcggagat gtcttcgagc cgcaaaggct tcactgcttc cagcgatcgc
      601 agtcccttga agagcgcctc cttgaacgtg cggccgatcg ccatgacctc gccgaccgac
      661 ttcatctgcg tgcccagcac gtcctccgct tcgcgaaact tttcgaagtc ccatttggga
      721 atctttgcca cgacgtaatc gatggttggt tcaaacgaag ccggtgtctt gcgcgtgatg
      781 tcgttgggaa tctcgtcgag cgtgtaacca acggcgagct tggcggcaat cttcgcaatc
      841 ggaaaaccgg tcgcttttga agcgagtgcc gaggagcgcg agacgcgcgg attcatttcg
      901 atgatgcgaa tgtcaccgtt ttcaggatta aggccgaatt gaatgttcga cccgccagtt
      961 tcgacaccaa cttttctgat cacgcgaatc gccatgtcgc gcatcaactg gtattcacgg
     1021 tcggtcaacg tttgcgccgg cgcgacggtg atcgagtcgc cggtgtgcac acccatcgga
     1081 tcgaaatttt cgatcgagca gatgatgacg acgttgtcag ccagatcgcg catcacctcc
     1141 agctcaaact ctttccaacc caaaatcgac tcttcaatca acacttcgtg aatcggcgaa
     1201 gccgccaagc cgccgcgcac gatctcttca aactcttccg gattgtaagc agtgccgccg
     1261 cctgttccgc cgagcgtaaa tgatggtctg ataatagccg ggtagtttgt cagcgcgacg
     1321 atttctcgcg cctcatccca tgacgtcgca aaccctccgc gaggcatctc cagtccggcc
     1381 tcgtccattg cctgtttgaa gagcaggcgg ttctcggcaa ccttgatcgc atcgcgattc
     1441 gcgccgatca tctcgacgcc gtactcgtcg agcacgccgg cgtccgaaag tgcgatggcg
     1501 aggttgagcg ccgtctggcc gccgaccgtc ggcaggagcg cgtcgggacg ttcgcggcga
     1561 atgatttcgg taactgaagc gacgttgagc ggctcaatgt aggtgacgtc ggccgtttcc
     1621 gggtcggtca tgatcgtcgc cgggttcgaa ttaaccagga cgatctcgta accctcctgc
     1681 cgcagcgctt tgcacgcttg cgtgccggag taatcaaatt cgcacgcctg cccgatcaca
     1741 attgggcccg agccgatgat cagaatcttg tgaatgtcag tccgtttggg catctctagc
     1801 taatgtccgg tctacgtcgt ctccggtaat cctctccgtc aatcaccacg ttggtacaca
     1861 tttcatagag acggctacgc aatcgatagg tgattcgttc ttcgagttcg ttgcttttgt
     1921 cgagatagtt cgtggtaaag attgtcagct ttttgtcgtt gtagcgtttg ttgatgatct
     1981 ggtacatcgt gtcgcgcacc cactcggtgg ggacagtcgc gcccaattcg tcgagcacga
     2041 gcacgtccgc ctgataaacg ggcgcgagca ctttcagttc ggatgatttg gagatggggt
     2101 tgtatgaatc ctggatctct ttcaacagcg agccgaattc gtagaagagg ccggcgaaac
     2161 ctttatcgat caagtctctg atgatcgaga cggcgagatg cgtcttgccc acaccggctg
     2221 gacccatgaa caacaatcca cggtcaacgg ccggatactc gttggcaaat ttgtgtgcgt
     2281 gacgaagcgc gtggtcctga gaagtgccgg gatcgctgat gaagttaaca aacgaacact
     2341 tctggtagcg gcgtggaata cgcgcagcag ccatcaactt ccgggaacgt tcactgcgac
     2401 ggcaaggaca aggaatcgct ccctttaccg ggtctaactt agtcccggtg ccaaagcact
     2461 cttcgcaaac ctctgtactc tctttcttac ggagttcgct ttcgtcgatc accattcacc
     2521 tgaatgaaat aaggtagacc ttatagtacc ttatattacc tttgagatat aacaggtgtt
     2581 gagttttaca ggagagataa aacaacggat gaatttgttg cagtcgttac tcagaaagct
     2641 cggattgact aatgcccgag ctgagcgcca tcccattcgc gtgtttctgc tggacgacga
     2701 caaacgacgc caccgctggt tttcggatcg cttcaaggga gacttcatcg atatcgcgca
     2761 ggacgtcgct gaagcgcagc gtcttttgag cgcaagttct tacgacgcga tctttttgga
     2821 tcacgacctc catcccgaac actacaacac tgactcaact gacgatgagc gcaccggtta
     2881 tgcgatcgca gcctggttgt gcgcgaatcc ggaactgcaa cgcgcctcga ccatcctcgt
     2941 gcacacgcga aacgcagacg gggcaatgcg catggtgggc gaactgcgcc gcgccgggcg
     3001 tagtgcagat tatgttccgt ttccgatgtt ggcggagaga ataaaaactt actggcagcg
     3061 ttagcgtcgg atgggctcgc cggcactatg acccgaaaca cacgtcgccg ttcatgaatc
     3121 gggtcagcga ggtacgaaaa tcttgacagc gacgctcaac gcgacctaga ttcgctacgt
     3181 catcccactc ggcggctgac tcgcgcttct tcaccgcaac cttccgaaag gacttcaaaa
     3241 tgaacagagt catacgaatc actttgcttt gcacgcttgt tgtcaccgtg agtacagcat
     3301 caatagctca aagacgcagt gctaacaaag caacgcgcaa agtgcccacc actgccacgc
     3361 agatcttctg cgaaggtgac tcgattccca aaggatacgt aattgtaggt tacaagtcct
     3421 cagccaaatg tggcgacaat tctcaactcg taatcaggaa acccgatgag gctgacatcg
     3481 tttgcagtgc ttccccaatt cccgacgcat accgtgttgc taatcagctg tcctctccag
     3541 attgcgtaac gaagggcaca aactcttcgc taaatgcttt gagcatcgta gcgaatcgtg
     3601 caaacggttc tcaacgacgt acggcgtcgc gcgccgggga cagcgctctc gctcgcgcat
     3661 tcgagagccg aaccagcgac atacaagtca aaggcgcagg caccgtcatt cgcgttctgc
     3721 cagacgatct caaggggtca cgacaccaac gattcatcgt tcagcttgcg tcgggacaga
     3781 cactgctgat tacgcataac ctcgacctcg ccccgcgaat cgacagactc aaagtaggcg
     3841 acagcgtcag cttttacggt gagtacgtat ggaacgaaaa aggcggcgtc atccattgga
     3901 cacaccatga cccgcaaggt aggcatgtcg caggttggtt aaaacacaac ggcaagacgt
     3961 ttcagtgatc aacggatcaa cgacggaacc tcatgccgag aaattgacca gatgattaaa
     4021 gcatcatggt tttgcgacgg tgctcgcttg acctgaggct gcgggcggag cgagtttcgc
     4081 cggcagtgcc tgcagcgtaa gatatggtaa gtctctgaat tcgacacgca ttgaagcgga
     4141 ttcgacgaac accatgtaaa gtcccggttg aggcgcttta acggtaattt catagacacc
     4201 gttaccaaga gattttgcga tgtcccggcg ctgccagatc cccggcgaga ggaatgtcag
     4261 cacgtgcaca tcagtcaggt ccgctttggg cttgccagtg gccgtatcga ataactggaa
     4321 gcgaagcggg aaatcctccc cgaccggcaa tctcctttcc ttggtttggt gctcgatccg
     4381 caaagcgacc ttttgctctt cttttaaaag cgggttcggc tccgccgtag cctcaaagca
     4441 atgagagact cgcggagagt cattcagaaa ggctatgtcg taatctccac tggcagggaa
     4501 cttgatcgtt gctgaataga tacctggatc ggtttcacgc aagctacgat cgacaatcaa
     4561 cacggacagc ggctcccgtt tgtaattttg gaagttaccc atcggagccg ccatgccttc
     4621 ggagtaataa tagagggtct tgtcgcccgg attagcgacg ataactgcgt tgccttccgg
     4681 cgccagcacc atcccctcgg cttggaccgt tcgagaagct ttctcaaacg gcgcctggcc
     4741 acctgggaat tccgtgacga taacatcctc gttgatcgtc gccagacgaa gcattgaaat
     4801 gctttctgtt ttcagcgatc tgacgaaagc aaacgtatcg ctaaagatga tttgatcagg
     4861 agcatttccc actttcgcgt cgtgaaggat gcgattcgaa gcggcatcga aaatggtaac
     4921 cgtgttctct ttagggttga gcacaaaccc atatcgccca tccggggaaa atcgaatcga
     4981 gcgcaaacct ggcttcgctt ttatctccgc cagcacctgt tggctttgct cgtcgatgac
     5041 tgtgacgact ccttccgttt cgcttgtcac atagatagct ttgctcaact tagacaccgt
     5101 catcgaaaca gggtttcggc ccactttcac gtcgctgatc ttc
//
LOCUS       DASTEY010000146         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_580047, whole genome shotgun sequence.
ACCESSION   DASTEY010000146 DASTEY010000000
VERSION     DASTEY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580047"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFO72_02550"
     CDS             <1..489
                     /locus_tag="VFO72_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HET9478195.1"
                     /translation="KFLTLVNPEIPIPRFVASLTGISNEMVKRAPVFAEVAPRWLDFV
                     SDSVLIAHNAPFDTSFLNHEISRVYPGHRMVNPHLCTVKLSRRALPELGNHRLDTIAS
                     HFSIPIVSRHRAGSDALATAEIFLVLLTKLEETHGIRDLASARNFQFPEVISNDCSAA
                     SA"
     gene            complement(441..1085)
                     /locus_tag="VFO72_02555"
     CDS             complement(441..1085)
                     /locus_tag="VFO72_02555"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9478196.1"
                     /translation="MKSFLSPITKALGRFSEYLITYGAFGLFIVALLDSTFVPLPSSA
                     DALMLVLSTAHPSWMLLYAFMATAGSALGCWILYVVSRRAGARALSRFSQSKQQRVKQ
                     LIERYDMFAVLVATLLPPPFPFKLFVVSAGVFRFSLLRFMLASIVGRAFRFLLEGYLA
                     VRYGAEAKVILAKYYPWIALGLVAGIVLFVIVKRWSKRGQAEAAEQSLEITSGN"
     gene            complement(1082..1732)
                     /locus_tag="VFO72_02560"
     CDS             complement(1082..1732)
                     /locus_tag="VFO72_02560"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9478197.1"
                     /translation="MTDWLLSIARWLEVNVIGLPLYLAAPAMVIIGALDSSLLSLPEI
                     NDYLVVGRCYKDPTAAYYFPLFAAAGSVLGCNLLYSIVRRGGQAVLRKRFKLSQIKKV
                     ERAYERFGFLAIGIPAILPPPLPFKIFVATAGALEYPRWKFLLTVMIARSFRYYVEGI
                     LAVFYGRRVLIFLKDNGLVIVSIVGSLALIGLIIYIVVKRRRGPAAEYTDSEGTTD"
     gene            complement(1758..2621)
                     /locus_tag="VFO72_02565"
     CDS             complement(1758..2621)
                     /locus_tag="VFO72_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478198.1"
                     /translation="MNCLFRFLSVALLLCFASVAQAQQRPLITEDVDIIPPGTIRIEA
                     GIDFMQGAKYTVSGIRGDLTRVGVIGINFGMGPNVEFQIEGVAQNYLSINSRGPSAIP
                     LELAPGANSTNDTGDFTISAKFKLRNETRRGPSLGFRFGVQLPNSSQATGIGLNQTNA
                     YGSILVGKKFGQDGRFNTFGNLGIAILTAPTELFSQNDVITYGVAGIFRINKQFSVAG
                     EVNGRANTRPGDGPLGTESQAEARLGMQIRASGLRFDFAGIKGLTSFSPNSGFTVGVT
                     YDTPSVFTPVK"
     gene            complement(2787..>2973)
                     /locus_tag="VFO72_02570"
     CDS             complement(2787..>2973)
                     /locus_tag="VFO72_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478199.1"
                     /translation="MTPDKIRCVSTQSQSLQVYSRIDVSRLLVIDAEHAQAFGLPPNG
                     VEGALRRKDHGNAILYK"
BASE COUNT          712 a          848 c          803 g          610 t
ORIGIN      
        1 aagtttctta cgctggtcaa tcccgaaatt ccgattcccc gattcgttgc ctcgctgaca
       61 ggaatttcaa acgagatggt caagcgcgcg ccggtgttcg cggaagttgc tccgcgctgg
      121 ctcgacttcg tcagcgactc ggtactgatc gcgcacaacg cgccgtttga caccagtttt
      181 ctcaatcacg aaatctcacg cgtgtatccg ggacatcgca tggtgaaccc gcacctctgc
      241 acggtcaaac tctcgcggcg cgcgctaccc gaactcggca atcatcgcct ggacacgatt
      301 gccagccact tctccatccc gatcgtcagc cgtcatcgcg ccggtagtga cgcactcgca
      361 accgccgaga tctttctcgt cttgcttacc aaactcgagg agacccacgg catcagagat
      421 ctcgcgtctg cgcgcaactt tcaatttccc gaagtgatct ccaacgactg ttccgcagct
      481 tcggcctgac ctcgtttcga ccaacgcttc acaatcacaa acaacacaat ccccgcaacg
      541 agtcccaacg caatccacgg ataatacttc gccaggatca ccttcgcttc cgcaccgtac
      601 ctgacggcca aatagccttc gagcagaaaa cgaaacgcgc gtccgacaat gctcgccagc
      661 atgaaccgca acaagctaaa ccggaaaaca cccgcgctaa cgacaaagag cttgaaagga
      721 aatggcggcg gcaaaagcgt cgcgaccaac acggcgaaca tatcgtaacg ctcgataagt
      781 tgcttgactc gctgctgttt tgattgagaa aagcggctga gagcgcgcgc tcctgcgcgg
      841 cgcgaaacca cgtagagaat ccagcaaccg agcgcgcttc cggccgtggc cataaacgcg
      901 tagagcaaca tccagctcgg atgcgcagtc gaaagcacca gcatcaacgc gtcggcactg
      961 ctgggcaaag gcacaaacgt cgagtcgagt agcgcaacta taaacagccc gaacgcgccg
     1021 tatgtgatca ggtattccga gaatcgacca agtgctttag tgatcggaga cagaaatgat
     1081 ttcaatcagt agtaccttca gagtcggtat attcggccgc cggacctctg cgccgtttga
     1141 ccacaatgta aataatcaaa cctatcaacg ccagcgaccc gacgatgctc acaatcacca
     1201 ggccgttgtc tttcaaaaag atcaacactc tgcggccgta gaagactgcg agaattcctt
     1261 cgacgtaata gcggaacgag cgcgcaatca tcaccgtcaa caaaaacttc caacgcggat
     1321 actcaagcgc tcctgccgtg gcgacaaata tcttgaacgg cagcggcggc ggcagaatcg
     1381 ccggaattcc gatggccaga aaaccgaatc tttcgtaagc gcgttcgacc tttttgattt
     1441 gggaaagctt gaaacgcttg cgcagtactg cctgtccgcc gcgtcgcacg attgaataga
     1501 gcaggttgca accgagcacc gatccggcgg cagcgaagag tggaaagtaa taagcggcgg
     1561 ttgggtcctt gtaacaacgt ccgacgacga ggtagtcgtt gatttccggc agcgacaaga
     1621 gcgatgaatc gagtgcgccg atgatgacca tcgccggcgc ggcgaggtag agcggcagtc
     1681 caatgacgtt tacttccaac catcgcgcaa tcgaaagtaa ccagtcagtc attctgaaat
     1741 gcgtatcttg gagcgaacta ttttaccggg gtaaagactg aaggtgtgtc gtaggtgact
     1801 ccgacggtaa accctgagtt tggactgaaa ctggtgaggc ccttgatgcc tgcgaaatcg
     1861 aagcgcagtc ccgaggcgcg gatctgcatt cccaaacgcg cctccgcctg cgattcagtg
     1921 cccaatggac catcgccagg gcgtgtgttg gcgcggccgt taacttcacc ggcgacgctg
     1981 aactgtttat tgatacggaa tattcccgcc acaccgtagg taatgacgtc gttctgcgaa
     2041 aacagttcgg tgggcgcagt cagaattgcg atgccgagat tgccaaacgt attgaagcgc
     2101 ccgtcctgac caaacttttt gccgacgaga atcgagccat acgcattggt ttgattgaga
     2161 ccgatgccgg ttgcctggct cgagtttggc agttgtacgc cgaaacgaaa gccgagcgac
     2221 gggccgcgac gcgtttcgtt gcgcagtttg aatttggcag agatggtgaa gtcgcccgtg
     2281 tcgttggtgg agttggcgcc gggcgcgagt tcgagtggaa ttgctgaagg gccgcgcgag
     2341 ttgatgctga gataattctg ggcgacgcct tcgatttgaa actcgacgtt tggccccatg
     2401 ccgaagttga tgccaatcac gccgacgcgc gtcagatcac cgcggatacc cgagaccgtg
     2461 tacttcgcac cctgcatgaa atcgatgccg gcctcgattc tgatcgtgcc cggcgggatg
     2521 atatcgacat cttcggtgat cagcggccgc tgctgcgcct gcgcaacaga ggcgaagcag
     2581 agcagcagcg caacagaaag gaatcggaac aggcaattca tgcgtgtcgg attctaaacg
     2641 gcggttcact cgcggtcaat attgccttca cttgagagta aacgccatgg gctccgactt
     2701 tgtattctga gtccaaagat accctttgtc gcgccacttc tgtcggtatt cttctggatt
     2761 taccagaaaa caccaagtcg caatcatcac ttgtataaaa tggcgttgcc gtgatctttg
     2821 cgcctgagcg cgccttcgac tccgtttggc ggcagaccaa aggcttgcgc gtgttccgcg
     2881 tcgattacga gcagacgaga gacgtcgatg cgcgagtaga cctgcaacga ctgggactgc
     2941 gtactgacgc agcggatctt gtctggtgtc atc
//
LOCUS       DASTEY010000147         5032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_712089, whole genome shotgun sequence.
ACCESSION   DASTEY010000147 DASTEY010000000
VERSION     DASTEY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712089"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1145)
                     /locus_tag="VFO72_02575"
     CDS             complement(75..1145)
                     /locus_tag="VFO72_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478200.1"
                     /translation="MHLPTRYAAKITAASVSLFVAFAFSFSVTGQDKPAPANPAQQGP
                     AQSQTPAQTSQSPDPTASPAQKQKLGSELDDRSLITNTDLITLTVTVTDTYGRYVSGL
                     SKSAFAVFDNKQQQEITFFSDDDSPVSVGVIFDVSGSMSGDKVKRARDALAKFIQTSH
                     DADEYFLIAFNSRAQLLLDRTRDGDSILNKLTFVETKNQTALYDACYLGVEKVQRGAH
                     PKRALLLISDGQDNDSRYTFNEVRKLLKESDVTLYAIGILSGNDAGSALGMEGQGVLD
                     ELSGVSGGKAFFPRSAAEMDDIFEQIALELRHQYSIGYKPESFVNDGKWHRVKVKVTP
                     PRGLPRLFVRSKEGYYATAGVR"
     gene            complement(1397..2380)
                     /locus_tag="VFO72_02580"
     CDS             complement(1397..2380)
                     /locus_tag="VFO72_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9478201.1"
                     /translation="MLQIITGFWISRAVYVIAKLGISDLLQTGPKSVDELAKATETHM
                     PALFRILRALASAGIVKRESDGRFALTPLSETLVTGVPGSMRWFIVSELGQEHYPAWG
                     NLMHSVKTGEIAFDNHFGMDIWAYFKQNPEDAAVFNDSMSGMTAAVNEAITSLYDFSQ
                     FDKVIDIGGGHGGLITSILKQNPKTKGVLFDAAQVIEGARPRLEQTGVADRCEAIAGD
                     FFKAVPAGGDVYVMKWIIHDWDDEKAVAILKNCRSQMQPNGRLIVVDCVVPEGDQPDF
                     SKTFDLNMLVMTGGKERTEAEFDQLFAAAGFKLLRVIPTGLPPYIVEGQPI"
     gene            2522..3763
                     /locus_tag="VFO72_02585"
     CDS             2522..3763
                     /locus_tag="VFO72_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9478202.1"
                     /translation="MAEFICRLGTPAGEVVTRTVEAPGVQEARARLEREGFRVFNVTP
                     PKTSGVTALTRFGRPGAHPRVKANDFLLFNQQLAALIRAGIPILQAISMLRKRAASAR
                     LRTVLGDVEEQIRGGAALSQAFAAQAGVFPRIYTASILAGERSGALDEVLSRYVNYMR
                     RNVALRRKIRGALAYPMFLLFASACMVTFLTVYVVPRMSELFAGFGNELPTVTQIVVG
                     ISRWLSTNVFWFGPLIIGGAVLLSIWSRTPAGKLQISALILKIPLAGSLAVQLSVAQA
                     TRSLATLLAGGITLVESWEIASESITNLELRRRSSSILPMIREGRSFTESLETAGWMP
                     LLAIDMIGIGERSGSLREMLDEVAVFYDAESEVRLEQLTTTLEPAILVVMGGVVVTIL
                     LAIYLPIIQSISSGAGVTGVH"
     gene            3764..>5032
                     /locus_tag="VFO72_02590"
     CDS             3764..>5032
                     /locus_tag="VFO72_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET9478203.1"
                     /translation="MEQVSEGKNPMVQTVSPSVQNGDDHVPPEVKAAKELARRYRLPF
                     VDLLPPDRESPIDYNLFTEVPVDLMVRHQFVPLKRDEKGLHIAMADPTDLERLDELSS
                     TLRTRVIPHVATAGAVEVVLRKGDTTQRVLQEAASGFRISLVRETEHGEEVLDLDRLA
                     TDSDMSPIIKLVDTIIYNAMESRASDIHIETRDTEVQVKYRIDGALYAKVDPIDLAYH
                     QTLISRIKVMSELDIAERRVPQDGRFRVRYKGRNVDFRVSIMPTIHGEDAVIRILDKE
                     QINEEFRNLNLNVVGFAEDDLRKFRRYIAEPYGMVLVTGPTGSGKTTTLYAALNEIRN
                     EEDKIVTIEDPVEYQLHGITQIPVNEKKGLTFDRGLRSILRHDPDKIMVGEIRDTETA
                     QIAIQSALTGHLVFTTVHANNVIDVIGRFLN"
BASE COUNT         1054 a         1419 c         1450 g         1109 t
ORIGIN      
        1 cccgtgaagc ctactcgaag gacgggccct taaacaacgc gaagcggctg cggcgcaaaa
       61 ggtgtccgta aagcctaccg cactccggcc gtagcgtagt agccctcctt ggaacgaacg
      121 aacagccggg gcaaaccccg cggcggcgta accttaacct tcacgcggtg ccacttcccg
      181 tcgttcacga acgattcggg tttgtaaccg atcgaatact ggtgccgcaa ctccagcgcg
      241 atctgttcga agatgtcgtc catctccgcg gcggatcgtg ggaaaaacgc cttcccgccg
      301 gagacgcccg acagctcatc gagcactccc tgaccttcca ttccgagcgc gctgccggcg
      361 tcgttgccgc tcaggatgcc gatcgcataa agcgttacgt ccgattcttt caaaagcttt
      421 cgtacttcat tgaacgtgta gcgcgaatcg ttgtcctgcc cgtcgctgat cagcagcaac
      481 gcgcgtttgg gatgagctcc gcgctgcact ttttcgacgc cgagataaca cgcgtcgtaa
      541 agcgccgtct ggttcttggt ttcgacaaac gtgagtttgt tgagaatcga atcaccgtcg
      601 cgagtacggt cgagcagcaa ctgggcgcgc gagttgaagg caatcaggaa atactcgtcg
      661 gcgtcgtggc tggtctgaat aaacttggcc agggcgtcgc gcgcgcgttt caccttgtcc
      721 ccgctcattg agcccgagac gtcgaagatg acgccgacgg aaacgggcga gtcatcgtca
      781 ctgaagaaag taatctcctg ttgctgcttg ttgtcgaata cggcaaaggc gctcttgctc
      841 aggccggaca cgtaacgtcc ataggtgtcg gtgacggtga cggtaagcgt gatgaggtcg
      901 gtgttggtga tcaacgaccg atcatcgagt tcggaaccca gtttctgttt ctgtgccggg
      961 ctggctgtcg ggtccggcga ttggcttgtt tgcgcaggcg tttggctttg agcgggaccc
     1021 tgctgggcag ggttagccgg cgcgggtttg tcctgacccg ttaccgaaaa cgaaaacgca
     1081 aatgcaacga atagcgatac ggaagccgcc gtgatcttag cggcgtaacg agtcggtaaa
     1141 tgcatgtcac ccccggctac agtttcttcg attcggtttt tcaactcaac cggtcaggct
     1201 cgtagggaac ttgtcaacac agatgctcgt caggggagcg agtgatgcct gacacttaat
     1261 ctgagctgaa aaggacacgg ggagacggcc gcgcgcgttt cattttgctg tgttggcaat
     1321 gccattctag gacaggcaag ttgacgcgtg caagcctttc aaatctccgc cttgagatcg
     1381 gaaatctcag acgggactaa atcggttggc cctcgactat gtacggcggc agcccggtgg
     1441 gaatgaccct caacaacttg aatccggccg cggcgaacag ttgatcaaac tcggcttccg
     1501 tccgttcttt accgccggtc atcaccagca tgttgaggtc gaaagtctta gagaaatcgg
     1561 gctgatcgcc ttcgggtacg acgcagtcca ccacgatcaa gcggccgttt ggctgcatct
     1621 gactgcgaca gttcttcaga atcgcaaccg ctttttcatc atcccagtcg tggatgatcc
     1681 acttcatcac gtaaacgtcg ccgccagcag gcacagcttt gaagaaatcg cccgcgatgg
     1741 cttcgcagcg atccgccact cccgtctgtt caaggcgcgg ccgggcgccc tcgatcactt
     1801 gcgcggcatc aaagaggacg cctttggtct tcggattttg tttgagaatg gacgtgatca
     1861 atccgccgtg accgccaccg atgtcgatca ccttgtcgaa ctgcgagaag tcgtagagcg
     1921 acgtgatcgc ctcgtttacg gcggcagtca tgccactcat cgaatcgttg aaaacggcag
     1981 cgtcttcggg gttctgcttg aaatacgccc aaatgtccat gccgaaatgg ttgtcaaatg
     2041 caatctctcc ggtcttgacg ctgtgcatca aattgcccca ggcgggataa tgttcctgcc
     2101 cgagttcgga aacgatgaac cagcgcatcg aacccggcac gccggtgacc agagtttccg
     2161 aaagcggcgt caacgcaaac cggccgtcgc tttcacgttt aacaatgccg gcgctagcca
     2221 gggcgcgcag gatgcgaaat agcgcaggca tatgcgtctc ggtggctttc gccagctcgt
     2281 cgacgctctt cggtcccgtt tgtagtagat cggaaatacc gagcttggcg attacgtaaa
     2341 cggcacgtga aatccagaat ccggtgatga tttggagcat ttgttcctgg gcggcaattt
     2401 gttctttttg atttgcggga gcagtcacga gtagggttct ccttccagtt cgggaaactt
     2461 tccttttaca gacgcgtgat atactcccga ttcggttcaa ccgcacataa acgaattaac
     2521 aatggctgaa tttatttgtc gattaggaac gccggcgggc gaagtcgtaa cacgcaccgt
     2581 tgaagcgccg ggtgttcagg aagcaagagc acgtctcgaa cgcgaggggt ttcgggtttt
     2641 caacgtcacg ccacctaaga cctccggcgt gactgcctta acgcgatttg gccgtccggg
     2701 tgctcatccg cgagtcaaag caaacgattt tctcctgttc aaccaacagc tcgccgcgct
     2761 gatccgagcg ggaatcccga ttctccaggc catctcgatg ttgcgcaaac gcgctgcctc
     2821 ggcacggctg cgcactgtgc tcggcgatgt tgaagaacag atccgaggcg gcgccgcact
     2881 ttcgcaggcg tttgccgcgc aggccggagt ctttccccgc atctacacgg cttcgattct
     2941 cgccggtgaa cgatccggcg cgctcgacga agtgttgagc cgctacgtga attacatgcg
     3001 gcgcaacgtc gcgttgcgac gcaagattcg cggcgcgctc gcttatccca tgtttctgtt
     3061 gtttgcctcc gcttgcatgg tcacgtttct cacggtctac gtcgtgccgc gcatgtccga
     3121 gctgtttgcc gggtttggca acgaactgcc cacagtgacg cagatcgtcg tcggtatctc
     3181 tcgctggttg tctacaaacg tcttttggtt tggtccacta atcatcggcg gcgcggtgct
     3241 cctttctatt tggtcgcgca cgccggccgg caaacttcag attagtgcgt tgattctgaa
     3301 gataccgttg gcgggcagcc tggcagtgca gctatcggta gcgcaggcga cgcgttcact
     3361 cgcgacgctt ctggcgggcg gcattacgct ggttgagtcg tgggagattg catcggagtc
     3421 gattacgaac ctcgaactgc ggcgccgcag ttcgtcgatc ctgccgatga ttcgggaggg
     3481 acggtcattt acggagagcc tggagacggc cggttggatg ccgctactgg cgatcgacat
     3541 gattggaatc ggagaacggt cgggcagttt acgcgagatg ctcgatgagg tagcggtttt
     3601 ctatgacgct gagtcagaag tccggcttga gcagttgacg accacgcttg agcctgcgat
     3661 tcttgtcgtg atgggcggtg tggtagtaac gattctcctc gccatctacc ttccaatcat
     3721 tcaatccatc tcgagcggtg cgggagtcac cggcgttcac taaatggagc aagtctcaga
     3781 aggcaagaac ccgatggtcc agacggtttc accgtctgtt caaaacggcg acgatcatgt
     3841 gccgccggaa gtgaaagccg cgaaagaact cgcgcggcgt taccggttgc cgtttgtcga
     3901 tctgctgccg ccggatcgcg aatcgccgat tgactacaac ctttttaccg aagtgcctgt
     3961 cgacctgatg gtgcggcacc agttcgttcc gctcaaacga gacgaaaagg gcctgcacat
     4021 cgcgatggcc gatccgaccg atctcgaacg gctggatgag ctatcgagca cgctgcgcac
     4081 gcgcgttatt ccgcacgtcg caacggcggg tgcggttgag gtcgtgcttc gcaaaggcga
     4141 caccactcaa cgcgtgttgc aggaagcggc atccgggttt cgcatctcgc tggttcgtga
     4201 aacagagcac ggtgaagaag tgctcgatct ggatcggctg gcgaccgatt cggatatgtc
     4261 gccgatcatc aaactcgtcg acacgatcat ctacaacgcg atggagtcgc gcgcgtcaga
     4321 cattcacatc gaaacgcgag acacggaagt acaggtgaag taccgtatcg acggcgcgct
     4381 ctacgcgaaa gtcgatccga tcgatctcgc ttatcaccag acgctcatct cgcgtatcaa
     4441 ggtcatgtct gaactcgaca ttgccgagcg gcgcgtgccg caggatggcc ggttcagagt
     4501 gcgttataaa ggacgcaacg tcgactttcg tgtttcgatc atgccgacga ttcacggcga
     4561 agacgcggtg attcgtattc tcgacaagga acagatcaac gaagagtttc gcaatctcaa
     4621 tctcaatgtt gtcggtttcg cggaagacga cctgcgaaag tttcgccgct acatcgctga
     4681 gccgtacggc atggtgctcg tgactggacc aaccggttcc ggcaaaacga ccacgcttta
     4741 cgccgcgctc aacgagatca gaaacgaaga agacaagatt gtcactatcg aagacccggt
     4801 cgagtatcag ttgcacggca tcacgcagat tcccgtcaac gaaaagaagg ggctgacctt
     4861 cgaccgcggc ttacgctcga tcctgcgtca cgacccggac aagatcatgg tcggtgagat
     4921 tcgcgacacc gaaaccgcgc agattgccat ccagtcggca ttgaccggcc acctcgtgtt
     4981 caccaccgtt cacgcgaaca acgtgatcga cgtgatcggg cgctttctga ac
//
LOCUS       DASTEY010000148         4901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1601820, whole genome shotgun sequence.
ACCESSION   DASTEY010000148 DASTEY010000000
VERSION     DASTEY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601820"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1395)
                     /locus_tag="VFO72_02595"
     CDS             complement(226..1395)
                     /locus_tag="VFO72_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9478204.1"
                     /translation="MNSLPVKILTAVLTFAIGVAVAGVWLTRWIDPVIDPVALNPPAA
                     RLEMVFVLDTTGSMGGLIEGAKQRIWGIVNEVMQASDSSVRIGLVAYRDRGDQYVTQV
                     LPLTEDLDKVYTTLMDYKAAGGGDGPEDVRTALAEGLYKAGWSNAAPDVSQIIFLVGD
                     APPHDDYRDTTDTLTTAAMAVQKGIIVNTIQCGSSVDTTRAWQAIAQRGNGQYFAIAY
                     NGGVQTISTPYDEELGELASRLGATFLAYGFGGAADAEARRAEVAASAAAVEGRVMHA
                     LPSAKAERALNKVLSKEAYVGDLLQSIENGSVKLESVKVADLPAELQTLDPAERQAEI
                     EKRLALRRDIRAQIMSLSKQRDAFVAEERKKSSKKDGFDAVVSKTLKEQMARKKD"
     gene            complement(1545..2099)
                     /locus_tag="VFO72_02600"
     CDS             complement(1545..2099)
                     /locus_tag="VFO72_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478205.1"
                     /translation="MRIIFSLLLIGVLLTGAFAQRPRTMEQEPAKTPAPKPAPATVKA
                     KYEGGVFGYNKTMEGTLTFDDVNNRLLFKDKKPPKEISIPYESITSAFADTQKRRPAA
                     ATVVGHIPTIYSLPARFIKTKVRYLTLQYSDPDSRVTGVTSFKLENKEMLQSVLATLA
                     QKAGMTLRGDIYVKNRDDSSKLTP"
     gene            complement(2126..2377)
                     /locus_tag="VFO72_02605"
     CDS             complement(2126..2377)
                     /locus_tag="VFO72_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478206.1"
                     /translation="MTDLAATARAFATYSLVPYLGILFCPGAVLVGTVGVFRSYSSQH
                     TRAARTACYLSTIIGVVVLIAQLLLWWILYQVPAWAIAQ"
     gene            complement(2490..3743)
                     /locus_tag="VFO72_02610"
     CDS             complement(2490..3743)
                     /locus_tag="VFO72_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478207.1"
                     /translation="MSRFTFIAAFLLLSVAASQAQSTRPRRAAKSPQKSNTTANKSAS
                     EPLLRPEPTPSPTARRTSSNDSLLDVQPVKPVVNTVAAADTRQAYLLIEQKQFAAAAK
                     AAKALTEQHPNDAEAWKIAGFAELNLKQYAEAVKDLEKAVELQRRSKQDDPNTESALG
                     QAYLLSERYQEALPLLVAATARNASKPDALIVYYRGVAEYKTGKTTDAEKSFNTVIKL
                     NPKDSLSLFYLAQISLAKNDLDGAIASLNRATVNDARMTSAWTLLTSSYLRRAALSTD
                     QAKADADYINAVRAGEGLIKLRTDAEAITLFGQALIGSKQYARAAAALERAAASQDAT
                     GVTFYLLGVAHSRAANFPKAIAALENAAKKSPEAVNVYRELGYAYEVSKQYAKALAAY
                     QKGAALAPNDADFKESIERVTPFAK"
     gene            complement(3917..>4901)
                     /locus_tag="VFO72_02615"
     CDS             complement(3917..>4901)
                     /locus_tag="VFO72_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478208.1"
                     /translation="PMFPTQGQTFAIIIPGMIAAPLTNLGSRIVSPAIANFFRPNAPN
                     YFLAQALSGGLVTPAVLNSALAGSLRTPGTISPFGSINAQTSDGNSSYNALNVDLKKR
                     FSSHFQFLASYTWSHSIDDSSDLQTLLLPQDNRNFGAERADSLFDQRHRFVFSAVIAS
                     PSSWAQGGGFHRALSNFTIAPIFEWSSGRPFNILSNQDTNNDQSNQTDRPSVLADGTL
                     CVPGTQGCVGIITNGRFTSGSLGRNFGLTHDYISLDLRVAKLVPLGERVRLELIAEGF
                     NLFNRFNEAAASPFIDDVRAFNLRAGNGRYYSRPTAAFDPRQFQFGLKLNF"
BASE COUNT         1011 a         1392 c         1379 g         1119 t
ORIGIN      
        1 agtcgcgcaa agcgacttat cctgcgctat acggtatcga ggaagctcgt gagaaagccg
       61 ttaaagctca tcgagcggcg tgcgcagcgc tcgaacagat cgatcgaccg accgacgtgt
      121 tgcaccgcat agtcgacttc atcctgcacc gcacaggtta actcaaaatc cgtgctcagt
      181 ccgtgtaatc cgtggcccta ctcggccgca gatcacgcgg actatttaat ctttcttccg
      241 cgccatctgc tctttcagcg ttttcgatac gaccgcatca aatccatctt tcttgctgct
      301 cttcttccgc tcctcggcaa cgaatgcgtc tctctgtttg gacaacgaca tgatctgcgc
      361 tctgatgtca cgccgcagcg caaggcgctt ctcgatctct gcctgacgct cggcggggtc
      421 aagtgtttgc aactctgccg gcaggtctgc aacctttact gattcgagtt tcacggatcc
      481 gttctcgata ctctgcaaca ggtcaccgac gtaagcttct ttgctgagca ccttattaag
      541 cgcccgctct gccttggctg acggcagagc atgcataaca cggccttcaa ctgccgccgc
      601 actcgcggct acttcagctc gtcttgcctc ggcgtccgcg gctccgccaa agccgtacgc
      661 aaggaaggtc gcgccgagtc tcgaggccag ctcgcccaac tcctcatcgt agggcgtgct
      721 gatagtctgc acgccgccat tgtaggcaat cgcaaagtat tgtccgtttc cccgctgagc
      781 aatcgcctgc cacgcgcgtg tcgtgtctac cgagcttcca cattgaatcg tgttaacgat
      841 tatgcctttc tgtacagcca tcgcggccgt ggtgagggta tcagtcgtat cgcggtagtc
      901 atcatgcggc ggagcgtcgc ccacgagaaa gatgatttgc gacacatccg gcgccgcatt
      961 cgaccagccc gctttgtaca gaccttcggc aagcgcggtt cgcacgtcct ctggaccatc
     1021 gccgcccccc gcagccttgt agtccatcag agtcgtgtag accttgtcca aatcctcggt
     1081 gagcggcaaa acctgggtca catactggtc cccgcggtcg cgataggcga cgagtccgat
     1141 gcgcacggac gaatccgatg cttgcatcac ctcgttgacg atgccccaga tccgctgttt
     1201 cgccccttca ataagcccgc ccatcgagcc cgtcgtatcc aacacaaaaa ccatttcgag
     1261 cctggcagca ggagggttca gcgcaacggg atcgattacc gggtcaatcc agcgcgtcag
     1321 ccagacgccg gcaacggcaa caccgatggc aaacgtgagt acggctgtca ggattttgac
     1381 gggcagcgaa ttcattgaag acctccctcc gcgcggagct ttggaacagg gggaacagga
     1441 atttgttcat tagacgtttg actgcggaat tcgttcccgc aagtacgcaa aaaaagaagc
     1501 gggccgcagc ccgcctctcg tcagtttccg ttttgtgagt caaatcaggg cgttaactta
     1561 ctggaatcgt cccgattttt gacgtaaatg tcgccgcgaa gcgtcatgcc ggctttctgc
     1621 gcgagtgtgg ccaacaccga ttggagcatc tctttgttct cgagtttgaa gcttgtgacg
     1681 ccggtaacac gtgaatcggg atcgctgtac tgaagcgtca ggtaacgcac tttggttttt
     1741 ataaagcgtg ccggcagcga atagatcgtc ggaatatggc cgacaactgt cgccgctgcg
     1801 ggccgtcgct tttgcgtatc ggcaaaggcc gaagtgatcg actcgtaggg tatcgagatc
     1861 tctttcggtg gctttttgtc tttgaatagc aaccggttgt taacgtcgtc gaaggtcaac
     1921 gtgccttcca tggtcttgtt gtaaccgaag acgccgccct catacttggc tttcacggtt
     1981 gccggtgcgg gcttgggcgc tggggtcttg gccggctcct gctccatcgt acgtggtcgt
     2041 tgcgcaaatg cgccggtgag cagcactccg atcaggagca gagaaaagat aattcgcatg
     2101 ttgcctcctt agccggtaac tgaattcatt gagcaatcgc ccacgcaggc acctggtaca
     2161 gtatccacca cagcagcaac tgagcaatca ataccaccac gccgatgatg gtgctcaggt
     2221 aacaggccgt acgcgccgca cgtgtgtgtt gcgagctgta agacctgaac acgcctaccg
     2281 tcccaaccag caccgcgccc ggacagaaca gaatgccgag gtacggcacg agcgagtaag
     2341 tggcgaacgc ccgcgcggtg gctgctaagt cggtcatctt tgaacttggt tctttgttct
     2401 ttgtgccttg ttctttgaac cttacttttc gcgctcagag ctttcgggat taacgctctt
     2461 acaaagtaca aagtactaag cattgaatac tacttagcaa acggcgtcac gcgctcgatc
     2521 gattctttga aatcagcatc gttgggagcg agtgccgcgc ccttctggta tgcagcgagc
     2581 gccttcgcgt attgtttgct cacttcgtaa gcatagccga gttcccgata aacgttgacg
     2641 gcctcaggtg atttcttcgc tgcgttttcc agagcagcaa ttgccttcgg gaaattcgcc
     2701 gcgcgtgaat gtgctacgcc taacaagtag aacgttacgc ccgtggcgtc ctgagaggcc
     2761 gccgctcgtt cgagcgcggc tgctgctcga gcatactgtt tcgaaccgat caacgcctga
     2821 ccgaagagcg tgatcgcttc ggcgtcagtg cgaagtttta tcagcccctc accggcgcgc
     2881 acggcgttaa tgtaatccgc atcggccttg gcctgatccg tgctgagcgc cgcgcgtcgc
     2941 aagtacgacg acgtcaacaa cgtccaggcg ctggtcatac gcgcgtcgtt caccgttgcg
     3001 cgattcagcg aagcgatcgc gccatcgaga tcgtttttcg ccaacgagat ctgcgcgaga
     3061 tagaagagcg ataaggaatc cttcggattg agtttgatga ctgtgttgaa gctcttttcc
     3121 gcgtcggtag tttttcccgt cttatattca gcgacaccgc ggtaatagac aatcaatgca
     3181 tcgggcttac tcgcgttacg cgcagtggcc gcgacgagca aaggcaacgc ctcctgatac
     3241 ctttcggaga gcaggtaagc ctggccgagc gcgctttcag tgtttggatc gtcctgtttc
     3301 gaacggcgct gcaactcaac cgctttctcc aaatccttca ccgcttcggc gtactgcttc
     3361 aagttcaatt ccgcaaagcc cgcaatcttc caggcctccg catcgtttgg gtgctgttcc
     3421 gtcagagcct tcgcggcctt cgcggcggct gcaaactgct tctgctcgat cagaagatac
     3481 gcctgcctcg tgtcggcagc ggcaacagtg ttaacgaccg gtttgaccgg ctgtacatcc
     3541 agcaacgagt cgtttgaact cgtgcgccgc gctgttggcg agggggttgg ttccgggcgg
     3601 agcaacggct ccgacgccga tttgttggct gttgtgttgc ttttttgcgg tgacttagcg
     3661 gcgcgtctcg gccgggtcga ttgggcctga gaggccgcca ctgacaacag gagaaaggcg
     3721 gcgataaacg taaatctgct catggtgcaa acaattgtat gcgggagaag gttaggtttg
     3781 aaagctcaga acccagcgtt gctcgttaaa cacacttcgg atgccggagg actcgtttgc
     3841 gttgtgttta cgtcacagat ttcacggact aacacggaac caactctgct ccgtgtttgt
     3901 cagtggccgc aatagtttag aagtttagct ttaacccaaa ctggaactgt cgcgggtcga
     3961 acgccgcagt cggacggctg tagtagcggc cgttgccggc gcgcaggttg aaagctcgca
     4021 cgtcatcgat aaacggcgag gctgccgctt cattgaaacg attgaagaga ttgaaccctt
     4081 ccgcaatcag ctccaaacgc acgcgttcgc caagcgggac aagtttcgcg acccgcagat
     4141 cgagcgatat gtagtcgtgg gtgaggccaa aattgcggcc caggcttcca ctcgtgaacc
     4201 gaccattcgt gataatccct acacagccct gcgtcccggg gacacacagc gtgccgtcag
     4261 ccagtaccga cggacggtcc gtctggtttg actggtcgtt atttgtgtcc tggttgctga
     4321 ggatgttgaa cggccggcct gacgaccact cgaagatcgg tgcgatcgtg aagttggaga
     4381 gcgcccgatg gaacccaccg ccctgtgccc agctacttgg cgaggcaatt acggcactga
     4441 atacaaacct gtgccgttga tcgaatagcg aatcggcccg ctcagcgccg aagttgcgat
     4501 tgtcctgtgg caacaaaagc gtttgcagat cggacgaatc gtcgatcgag tgagaccagg
     4561 tgtaggacgc gagaaattgg aagtgactcg agaagcgctt cttcagatcc acgttcagcg
     4621 cgttgtagct ggaattaccg tcggaagtct gcgcgttgat ggagccgaat ggagagatcg
     4681 ttccgggagt tcgcagtgaa ccagccagcg cgctattcaa cactgcgggc gtaaccagac
     4741 cgccggaaag cgcctgggcc aggaagtagt taggcgcgtt tggccggaag aagttggcaa
     4801 tagccggact gacgattcgg ctgccgaggt tagtcagagg tgcggcgatc attccgggaa
     4861 tgatgattgc gaaagtctgt ccctgggttg gaaacatcgg g
//
LOCUS       DASTEY010000149         5787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_448163, whole genome shotgun sequence.
ACCESSION   DASTEY010000149 DASTEY010000000
VERSION     DASTEY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448163"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="VFO72_02620"
     CDS             complement(<1..1756)
                     /locus_tag="VFO72_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET9478209.1"
                     /translation="MWRRLIFFAFVLTAVEADAQQTRPVLISHADSTRAIAFDSVTRR
                     REPFNTTAEIPFGSDSATRIMLFAMNLQPNELPSADAEDANHNLHQLTVEHVAPVPNH
                     PWVSSVVIRLPQSLPANGDVLVRINYGNLASNRVRIGIGQVGGGPPDDLSAIPTPGLP
                     APFDPPSAITATNLTSTDVQTILQQAASAAASLSKAVTIVVTDREANILGFFAMTGAP
                     ATTTIRSVGAFGQGLEGTVAPAAEAATAKAATAAFFSTTGNAFSTRTAGFIIQEHFPP
                     GISFRAGGPLYGVQFSSLPCSDIKKPSARLGLSADPGGLPIYKNGLPAGGIGIEGDGL
                     YTVDRDPRDNDQPFEELIAASGIRGFEAPAGIRGDTILVDGIRLPFTNVTNAPTPTTT
                     ASITGSIITPPPASEFTPQVVGGIAGEVSPRFFPFIGGTAPSGNALSAAEVLTIISHA
                     AQQANITRAAIRQPLGSNARVTIAVVDAAGVVLGVFRQQDAPVFGFDVAVQKARTAAF
                     FSSANAATALRAAGLGAYVDRAIADGLRLDGSIAFSDRATGFLHRPFFPDGIDNTAAG
                     PFSTAIGSWSPFNVGLQLD"
     gene            1884..3842
                     /locus_tag="VFO72_02625"
     CDS             1884..3842
                     /locus_tag="VFO72_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9478210.1"
                     /translation="MVRTQTGAPVPRAVVVVTNQVTRETKRVRTNADGRYSIKLPAGA
                     YRLTLDQPHVTVFDKDKNYGDFAIVRGDTLENVIVEASKEIEVEIRVAESPAGDKSGT
                     QKRETPDRWRIIFPEYDRYGDRGARGRDIPFKRGRWWDPYNQNVLKGDYPIKGDKLFV
                     ILTAVSATNVEQRRVPTPSGVSTVNPISAEFFGRPEEFAAAETLQFSFELFHGDTTFK
                     PRDWAIKFSPTFSVPNYLDARENGVVNIDVRRGTNRTDWHASLEEAFGEVKLFDVNAN
                     YDFVSVRAGIQPFVSDFRGFIFSDNNLGARVFGAADNNRVQFNGAYFSMLEKDTNSGL
                     NRFDTRHQNVYIANIFRQDFIRKGYTIQGSFHYNDDRRGVKFDRNGFLVRPAPIGDVR
                     PHAVKVGYVGFSGDGHLGRLNLSHSYYFAFGHDEHNPIAGRRVDVRSNLAAAELSVDK
                     DYLRFKGSFLWAQGDSNPADEKANGFDAIFDDPNFAGGQFSFWVRNGIRLTQTGVGLV
                     QPNSLLPSLRSSKTQGQANFVNPGIFIYNGGVDVEVTQRVKAVVNVNYLRFHRTEPLE
                     LVLFQNRIRHDIGFDYSLGVAYRPFLINNFTVTFGGSALQTGRGFRDIFTDASRNCPP
                     GVEDFCQPEVISPKKALYSLFAQMKFVF"
     gene            3850..>5787
                     /locus_tag="VFO72_02630"
     CDS             3850..>5787
                     /locus_tag="VFO72_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478211.1"
                     /translation="MTQIFLYLIAVITFGQAEAAQPKVEGCVSCHTMIEPMHKYGTTE
                     TLEKLKDGKDAVGLSCTACHGGNPASKLKDEAHVKPEFPDEWRRAGKYSGANPERSNT
                     LLNRESWEFVRFVNPGDLRVAAKTCGSASCHEVESKNVARSMMTHGAMLWGAALYNNG
                     SFHLKDPRFGESYSESGKPQTLLQATTDLTDEQRRAKGLLTLLDPIPRWEISQPGNVL
                     RVFERGGKRRLEVGLPDKEEEPGKPDKGLSVRGLGTAQRTDPVFLGLQKTRLLDPTLN
                     HLGTNDHPGDYRSSGCSACHVIYANDREPAHSAFYHAAGNLGSSQTADTSIPKNQSGH
                     PIKHQFTSQIPSSQCMVCHMHPGTNMVASYMGLTWWDNESDGSKLYPAKQHNPSQDEE
                     QEKLNRNPEAASLLGLWSNFEFLKDTGTPAFNALLRNTQFADAHGHGWLFRKVFKRDR
                     KGNLLDAPGNIVAADDPLKFSKAVHLTDIHLERGMHCVDCHFRQDSHGDGNLYNEPRA
                     AIEITCEDCHGTIKQKATLFTSGPAAAPATGNTLERRKSQKQPLIGQDLTRIRVRDES
                     GSRIPLFQRVTRDRKRKDEQGRDVDLKAGDIMQNSMVVPGRWWRVPQTLDTVTPNARD
                     YNEKSRYAKTIRKDNVTWGDVP"
BASE COUNT         1290 a         1513 c         1751 g         1233 t
ORIGIN      
        1 gatccagttg tagtccaaca ttgaaggggc tccagcttcc gatggccgtg ctgaatggac
       61 cagcggcagt attgtcgatt ccgtccggaa agaacggtcg atgtagaaat ccagtcgcac
      121 gatcgctaaa cgcgatagag ccgtcgagcc gcagaccgtc cgcaatcgcg cgatccacgt
      181 aagcgccgag accggcagct cgcaacgccg tcgccgcgtt tgcactcgaa aagaaagcgg
      241 cagttctcgc cttttgcacg gcgacgtcaa acccaaacac cggcgcatcc tgttgccgga
      301 agacgccgag cacgactccc gcggcgtcga caaccgcaat cgttacacgc gcattgctgc
      361 cgagcggttg gcgaatcgcg gcgcgcgtga tgttcgcctg ttgcgccgcg tgtgaaatga
      421 tggtcaggac ctcggcagcg gagagcgcgt ttccacttgg cgctgtgccg ccgatgaacg
      481 gaaagaaacg cggactcact tcacccgcaa tcccgccgac cacttgcggc gtgaactctg
      541 acgcaggcgg aggagtgata atcgaaccgg ttatgctcgc ggttgtcgtt ggagtgggtg
      601 cgttggtgac gtttgtaaac ggcaacctga tgccatcaac gagtatcgtg tcgccgcgaa
      661 ttcccgcggg cgcttcgaaa ccgcgaatcc ctgaggcagc tatcaattct tcaaacggtt
      721 gatcattgtc cctcggatcg cggtcgacgg tgtaaagacc atcgccttcg atgccgatgc
      781 cgcccgcggg gagtccgttc ttgtagatcg gcaaaccgcc tggatccgcg gagagaccca
      841 gacgcgcgga gggtttcttg atatcgctgc aaggcaggga agagaattgc acgccgtaaa
      901 gcggaccacc cgcgcgaaaa ctgattccgg gtggaaagtg ttcctggata ataaaacccg
      961 cggtgcgagt cgaaaaagcg ttgcccgtcg tactgaagaa cgcggcggtc gcggctttgg
     1021 ccgtcgctgc ttcggcggcc ggcgcgactg ttccttcgag tccctgaccg aatgcgccga
     1081 cactgcgaat cgtcgttgtc gcgggcgcgc cagtcatggc gaagaagccg agaatgtttg
     1141 cttctcgatc ggtgacaacg atcgtgacgg ccttactgag agatgccgcg gccgacgcag
     1201 cctgttggag gatcgtctgc acgtcggttg aagttaagtt ggtggcggtg attgctgatg
     1261 gtggatcgaa tggcgccggc aagccgggcg ttggtatggc actcaggtcg tccggtggtc
     1321 caccgccgac ctggccaatg ccaatgcgta ctcgattgct tgctagattg ccgtagttta
     1381 ttcggactag aacgtcgcca ttggcgggaa gactttgggg gagacggatt accacggatg
     1441 aaacccacgg atgatttgga accggcgcaa cgtgttcgac ggtgagttga tgaagattgt
     1501 ggttcgcgtc ttcagcgtcg gccgatggga gttcgttcgg ttgaagattc atcgcaaaga
     1561 gcatgatccg cgtagctgaa tccgaaccaa acgggatctc tgcagtagtg ttgaacggtt
     1621 cgcgacggcg tgtgacagag tcgaaagcga tggcccgagt tgaatcggcg tgactgatca
     1681 ggaccggacg tgtttgttgc gcatcagcct caacagcagt gagcacgaac gcgaagaaga
     1741 tcagtcgtcg ccacatcggc gcacaatgta tcacagattt tttctgccga atcggtgaaa
     1801 tatgtgataa aagcagagac cgcgtgatca tattactgat cgtagcaagt tccctcttac
     1861 agacgactgg cagccttaag ggagtggtcc gcactcaaac tggggcgccg gttcctcgcg
     1921 cggttgttgt cgttactaac caggttactc gcgaaaccaa acgtgtgcgg acgaatgcag
     1981 acggccgtta ttcgatcaag cttcctgccg gtgcgtatcg gcttactctg gaccagccgc
     2041 atgtcactgt tttcgataag gataagaact atggtgactt tgcgatcgtc cgtggtgaca
     2101 cgttggagaa cgtcatcgtt gaagcaagta aggagataga ggttgagatc cgcgttgcag
     2161 agagcccagc gggggataaa tcaggaacac agaagcgaga gacaccggat agatggcgga
     2221 taatcttccc ggaatatgat cgctatggcg atcgtggcgc gcgcgggcgt gatattccgt
     2281 tcaagagagg ccgctggtgg gatccttaca accagaatgt actgaagggc gactatccga
     2341 tcaagggcga taagctgttc gtgattttga ctgcggtcag cgcgacgaat gttgaacaac
     2401 gacgcgtgcc gacgccatcg ggcgtcagca ctgtgaaccc gatcagcgca gagtttttcg
     2461 gccgcccgga agaatttgcc gccgccgaga cgctacagtt cagttttgaa ctctttcacg
     2521 gcgataccac gttcaagccg cgcgactggg ccatcaagtt ttcgcctacg ttcagcgtgc
     2581 cgaactatct cgatgcgcgt gagaacggcg tagttaacat cgacgtgcgg cgtggtacga
     2641 accgtactga ttggcacgct tcgctcgaag aagcgtttgg cgaagtcaaa ctgttcgatg
     2701 taaacgccaa ctacgacttt gtctcggtgc gcgccggcat tcagccgttt gtttccgact
     2761 tccgtggctt catcttttcc gacaacaatc tcggcgcgcg cgtgtttggc gccgccgaca
     2821 acaatcgcgt gcagttcaat ggcgcctact tctcgatgct cgagaaggat acgaatagtg
     2881 gtttgaatcg cttcgacacg cgccaccaga acgtttatat cgccaacatt ttccggcagg
     2941 acttcattcg caagggttat acgatccagg gaagctttca ctataacgac gaccggcgcg
     3001 gcgtgaagtt tgatcgcaac ggttttctgg tgcggcccgc tcccattggc gacgtgcggc
     3061 cgcacgcagt caaggttggc tacgtggggt tcagtggaga tggacacctt ggacgtttga
     3121 atctgtcaca ttcgtattat tttgcttttg gtcacgacga gcacaacccg atcgcggggc
     3181 ggagagtcga tgttagatcg aacctggcgg cggcggagct gtcggtcgat aaggactacc
     3241 tgcgatttaa gggctcgttt ctgtgggctc agggggattc aaatccggcg gatgaaaaag
     3301 caaatgggtt cgatgcgatc tttgacgatc cgaacttcgc cggcggccag ttttcgtttt
     3361 gggtgcgcaa cggaattcgt ttgacgcaaa ctggcgtggg gttggtccaa ccaaacagcc
     3421 tcttgccgag tttgcgcagc agcaagactc aggggcaggc aaatttcgtc aaccccggaa
     3481 tcttcattta caacggaggt gttgatgttg aagtgacgca acgcgttaag gcggtggtga
     3541 acgtgaacta tctgcgtttt caccggacgg aaccgctgga gttggtgctg tttcagaacc
     3601 gcattcgcca cgacatcgga ttcgattaca gtttgggcgt agcctatcgg ccgtttctga
     3661 ttaataactt cacggtgacc tttggcgggt cagcgttgca aaccggccgc ggctttcgcg
     3721 acatattcac cgacgcgtcg cgcaactgcc cgcctggcgt ggaagatttc tgccagccgg
     3781 aggtgattag tccgaagaag gcgctgtatt cgctgtttgc gcagatgaag tttgtgtttt
     3841 aggccacaga tgacacagat ttttttgtac ttgatagccg tgattacctt tggacaggcc
     3901 gaggcggcgc agccgaaggt ggagggttgt gtgtcgtgtc acacgatgat tgagccgatg
     3961 cacaaatatg gcacgacgga gacgctcgag aagttgaaag atggtaagga cgcggtggga
     4021 ttgagttgta cggcgtgtca cggtgggaac ccggcgtcga aattgaagga cgaagcgcat
     4081 gtgaaaccgg agtttcctga tgagtggcgg agggcgggga agtacagcgg tgcgaatccc
     4141 gaacggtcca atacgctttt gaatcgcgag agttgggagt ttgttcgctt tgttaatccg
     4201 ggtgatttgc gggtggcggc gaagacgtgt ggcagtgcgt catgtcatga agtggagtcg
     4261 aagaacgtag cgcgcagcat gatgacgcac ggcgcgatgc tgtggggcgc ggcgctttat
     4321 aacaacggaa gttttcatct gaaggatccg cggtttggcg agagctactc tgagtcgggg
     4381 aagccgcaaa cgttgcttca ggcgaccacg gatttaacgg acgagcagcg gagagctaaa
     4441 gggttgttga cgttgttaga tccaatccca cgttgggaga tctctcagcc cggaaacgtc
     4501 ctgcgcgtgt ttgaacgtgg tggaaagcgc cgtctcgaag tcgggctgcc ggataaagaa
     4561 gaagaaccgg gcaaaccgga taagggattg agtgtccgtg gacttggcac ggctcagcga
     4621 acggatccgg tttttcttgg gttacagaag acgagactgt tggatccaac gcttaatcat
     4681 cttggcacca acgatcatcc tggcgactat cgttcctccg gttgctcggc gtgtcacgtg
     4741 atttacgcaa atgatcgcga gccggcgcac tccgctttct accacgctgc cggcaacctc
     4801 ggtagttcgc aaactgccga cacgagcatt cccaaaaacc aatcgggcca tccgatcaaa
     4861 caccagttca ccagtcagat tccgtcgtcg cagtgcatgg tctgccacat gcatcccggc
     4921 acaaacatgg tggcgagcta catgggtctc acgtggtggg acaacgagag cgacggctcg
     4981 aagctctatc ccgcgaagca acacaatccc agccaggatg aagaacagga aaaactgaac
     5041 cgcaatcctg aagcagcaag tctgcttggt ctgtggtcta actttgaatt cttgaaagac
     5101 acaggcacgc ctgctttcaa cgcgcttttg cgcaacacgc agtttgccga cgcgcacggg
     5161 catggctggc tgtttcgcaa agtcttcaaa cgcgaccgca agggcaactt gctcgacgcg
     5221 ccaggcaaca tcgtcgcggc tgacgatccg ttgaagttta gtaaggcagt tcatctcacc
     5281 gatatccatc tcgaacgcgg tatgcactgc gtcgattgtc acttccgcca ggactcgcat
     5341 ggcgacggca acctctacaa cgagccgcgc gcggctattg aaatcacctg cgaagactgc
     5401 cacggaacca tcaaacagaa agcgactttg ttcacctctg gtcccgcagc tgccccggcg
     5461 accggaaaca cgctcgagcg tcgcaagtct cagaaacagc ctttgattgg acaagacctg
     5521 actcggattc gcgtgcgcga cgaatccggg agcaggatcc cgctgtttca acgcgtcacg
     5581 cgagatcgaa aacggaagga tgaacagggc cgcgacgtcg acttaaaagc cggcgacata
     5641 atgcagaact ctatggtcgt gcccggtcgc tggtggcgtg tgccgcaaac gctcgataca
     5701 gtcacgccga acgcgcgcga ttacaacgag aaatctcgtt acgcgaaaac gatccgcaaa
     5761 gacaacgtca cctggggcga cgttcca
//
LOCUS       DASTEY010000150         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_942666, whole genome shotgun sequence.
ACCESSION   DASTEY010000150 DASTEY010000000
VERSION     DASTEY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942666"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFO72_02635"
     CDS             <1..392
                     /locus_tag="VFO72_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9478212.1"
                     /translation="LTCAALALAGTACTETATPTNTASPSVAASPAAPSPAASVDEFA
                     NARANYAKNCEGCHGPEGNGGLVKVDNKQIKVPSLHAEHAIKHTDEEIIDFITNGHEE
                     MPAFKDKMRPEEIIELVRFVRKVHQGK"
     gene            523..599
                     /locus_tag="VFO72_02640"
     tRNA            523..599
                     /locus_tag="VFO72_02640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Arg,seq:tcg)
     gene            645..1469
                     /gene="speB"
                     /locus_tag="VFO72_02645"
     CDS             645..1469
                     /gene="speB"
                     /locus_tag="VFO72_02645"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET9478213.1"
                     /translation="MPENSSTIEQRTKLAIIGIRYDENSSFTKGAADAPPQIRAALRS
                     DAWNLTSENGTDLSGDEIFFDAGDIEPVPGSDMFTLIENSVYTLIADGMAPISLGGDH
                     SITYPIVRAFARKYPNLSVVHFDAHPDLYDSYQGNRNSHASPFARIMEQKLVKRLVQV
                     GIRTFTAHQREQVKKFGVESIEMHRLTDRLELEFDSPVYISFDVDALDPAFAPGVSHR
                     EPGGMSTRQAIDLIQRLKGKVVGADIVEFNPRMDPLHITGTVCAKLLKEIAARMME"
     gene            complement(1578..2186)
                     /locus_tag="VFO72_02650"
     CDS             complement(1578..2186)
                     /locus_tag="VFO72_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478214.1"
                     /translation="MDKLVRKGAYTASLCLLVLLLATGAFAQGKGRGGGGGRGGGSSA
                     GGPPAGRGVDRGLDNSSSKSGGRADDGRNNASDKSKGRSDAGLARARRANLRRADDDL
                     RNHPGLPRALHTNANDLRAGYQAALAVNPDLTFGNYVAATRLSQNLGSRHSNITRDAI
                     LGGLASGRSMGQTLQDLGLNEREAKEARRRVDDEIKKAKKNK"
BASE COUNT          537 a          695 c          624 g          480 t
ORIGIN      
        1 ctctaacctg cgctgcctta gcgttagccg gaactgcctg caccgaaaca gcgacaccaa
       61 cgaatactgc atcaccgagt gtggctgcgt cgccagccgc gccttcgccc gctgcctccg
      121 tcgatgagtt tgccaacgcg cgagctaact acgcgaagaa ttgtgaaggg tgtcacggtc
      181 cagagggcaa cggcggcctg gttaaagttg acaacaagca gatcaaagtc ccatcactgc
      241 acgccgagca tgcgattaag cacactgacg aggagataat cgacttcatc acaaacggtc
      301 acgaagagat gccggcgttc aaagacaaaa tgcggccgga agagatcatc gaactggtgc
      361 gctttgtacg caaggtgcat caggggaaat gatttgagct tggagctttg tgctttgctt
      421 gagctcagct gaagtccccg caaagcgaac aaagtacaaa gtacaaagct ccccccttgc
      481 tgcctttacc aatccgtctg aaccatttaa cattccccgc gtgcacccgt agctcagttg
      541 gatagagcgt ttgacttcga atcaaaaggt cgcaggttcg agccctgccg ggtgtaccat
      601 aaggcccctg ttaacactcc atcgtacgca cccagaagcg tcgtatgccc gaaaattcca
      661 gcactattga acaacgcacc aaactcgcca tcatcggtat tcgctacgac gagaactcgt
      721 cgtttactaa aggcgccgcc gacgcgccgc ctcagatccg cgccgcactg cgttccgatg
      781 cgtggaacct gacaagtgag aacggaactg atctctccgg tgacgaaatt ttcttcgacg
      841 caggtgacat cgagccggtt cccggcagcg acatgttcac cttgatcgaa aactccgtct
      901 acacgctgat cgccgatggc atggcgccca tttcgcttgg tggagatcac tcgatcacct
      961 atccaatcgt gagagcattc gcgcggaagt atccgaatct aagcgtggtc catttcgacg
     1021 cccatcccga tctctacgac agctaccagg gcaatcgaaa ctctcacgcg agcccgtttg
     1081 cgcgaatcat ggaacagaaa ctggtgaaac gactggtcca ggttgggatc aggacgttca
     1141 cggctcacca acgcgaacag gtgaagaagt ttggcgtcga gtcgatcgag atgcaccggt
     1201 taactgaccg tctggaactc gagtttgatt cgcctgttta catctcattc gacgtcgacg
     1261 cgctcgaccc ggcgtttgcg cccggcgtgt cgcatcgcga acccggtggg atgtcgacgc
     1321 gacaggcgat cgatctgatt caacggttga aggggaaagt ggtgggcgcg gatattgtcg
     1381 agttcaatcc acgaatggac ccgttgcaca tcacgggcac agtctgcgcg aagttattga
     1441 aggagattgc cgcgcggatg atggagtaaa taggaacgag ttcctccctg ggaccgcagg
     1501 cgtctcgcct gctgtttggt tgagagctac tcgcgtctca agcaggcgag acgcctgcgg
     1561 tcccaggctg aaagactcta cttgttcttc ttagccttct tgatttcgtc gtcgacgcgg
     1621 cgtcgcgcct ccttcgcttc acgctcgttc aatccgagat cctgaagcgt ttggcccatg
     1681 ctcctgccgg aagccagtcc gcccaaaatc gcgtcgcgcg tgatgttcga atgacgagag
     1741 ccaagattct ggctcaacct ggtagccgcc acgtaattgc caaacgtcag atccggatta
     1801 acggccagcg cagcttgata accggcacgc agatcgtttg cgttcgtgtg caaagctctc
     1861 ggcagtcccg gatgattacg gagatcgtcg tcagcgcgtc gcaggttggc gcgtcgcgcg
     1921 cgcgcgagcc ccgcatcgga tcgacccttt gacttgtccg aagcattgtt gcgaccgtcg
     1981 tcggcgcgac cgccggattt gctggaagag ttatccagcc cgcggtccac acctcttccg
     2041 gcaggtggtc caccagcact acttccaccg ccgcgtccac cacctcctcc tctacccttt
     2101 ccttgcgcaa aggctcccgt tgccaacagc aacacgagca ggcacaagga cgctgtgtaa
     2161 gccccttttc gaaccaactt atccatcttc accgcctcct tagcgttgaa ttcaatcttg
     2221 agggggacaa gaggagagag aacaacaaat ctactacacc gcgccgccgc aaggccacgg
     2281 aaaaaacaaa atacaaggct gttgcaataa aaacgggcag gaagcggtcc atccgg
//
LOCUS       DASTEY010000151         4978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_547235, whole genome shotgun sequence.
ACCESSION   DASTEY010000151 DASTEY010000000
VERSION     DASTEY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547235"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..907)
                     /locus_tag="VFO72_02655"
     CDS             complement(38..907)
                     /locus_tag="VFO72_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478215.1"
                     /translation="MASGVSAIVTAYERIEQTLATLTVIQSCAPAPHEILVHVDDNRL
                     ACAGAICDAFPDVRVLVSRERVGPGGGRNKLLAAVTQDLVASFDDDSYPIDSDYFARA
                     QQLFAQHPNASVVCAALYHRGETIAFDERKIEWTADFCGGACVFRRSAFLAAGGYVPL
                     PVAYGMEEVDLALRLHSRGGRILTSSWLRVFHDTDLARHGDPRVTAGSIANLALLAYL
                     RYPVSLWWVGFTQCVNRVLWLLRNGRRRGIWTGVRMIPAHLRAHRQYRLPLAFHDVKS
                     YLALRRAPLAESS"
     gene            complement(900..1793)
                     /locus_tag="VFO72_02660"
     CDS             complement(900..1793)
                     /locus_tag="VFO72_02660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9478216.1"
                     /translation="MSCKARNWVFLGDSLTEGVGSKRVSHVSELARQLRTKSNGSVHE
                     FRLRQIETDAFRQIDFNVAGFMNVDGELKGAGSCRDLWLWNLACEGQTIDSDFAWLPL
                     IAALKPELVVAFRGGLESIIRPAVFRGGAWPWWVPASWRGYAAMDPRCYFSTTWWRKA
                     KQTSVDALKQKARLKLLKERNGLPLMDTEQLITHESSLLKSLRALGARVVVLGLLPVG
                     EDRFPGSPEHFKTVNARLRDAAEREGAEFFDWAAQLAQKGRRQDLFYRDSFHPNAAGA
                     RALAEILQAYLCRDLAATNHG"
     gene            complement(1812..2744)
                     /locus_tag="VFO72_02665"
     CDS             complement(1812..2744)
                     /locus_tag="VFO72_02665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9478217.1"
                     /translation="MKISAIVPTIGRPRSLRALLESVAAQTHRVDEVIVADASVDDQT
                     ERVLADFKSQSAFIVKRIAVHPPNAVRQRAAAISVAHGELFLFLDDDVVLEPDCVARM
                     IESLESNDCVVAVSADFNNQTWPAPTRMWQLYLRHVLKLKQDAWQGRVVGPLLRFGYN
                     SVPCEPCATEWLGSGNSLVRRSAFVAAGGFSDFFLHRCTINEDIDLGLKLRRVGTIMF
                     CPAARMAHHHASSGRVTRFMAAEDDLFNRFFILRRTMRFSLLRSFGLVLLYFTVETAS
                     QFLGCVRRLSFRGFVAPLTGRLRAFARLLIPLAR"
     gene            complement(2746..3615)
                     /locus_tag="VFO72_02670"
     CDS             complement(2746..3615)
                     /locus_tag="VFO72_02670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9478218.1"
                     /translation="MNKTVLGLFRRVPARAARHALFLIQERPELSDRWGYHIRPIHYY
                     EPLPDFRTITPEQIQRRREFSGIDFRWEAQLRLLRELESYAGELSESEVQNDYFSGLD
                     AVIYYSLIRRLKPRRIIEIGGGYSTRIAGKALAANQTGRLTSIEPFPERLNGSSLGVE
                     LITKRVEEIEVSFFASLKANDILFIDSSHTVKFGSDVCYEFLEILPALSRGVWVHVHD
                     VFFPHDYPAEWLLKRRLALNEQYLLEAFLAFNREFQIALANHWLALDHLAQVKRLWAS
                     PNSGSSSFWFYRT"
     gene            complement(3624..4796)
                     /locus_tag="VFO72_02675"
     CDS             complement(3624..4796)
                     /locus_tag="VFO72_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478219.1"
                     /translation="MLPHLLYVGDVPVEASYHGSALLHRLLVDYPVDRLTVIETAAPS
                     MPSRRLPNVNYLSHPLGKQRWLNTRFHAHAVAWYSRAAARRGPQISARLNGFACEGVL
                     TVAHGFGWLAAAALAARRKAPLHLMVHDDWPRVVNVRPGVRNWIDARFADVYRAARSR
                     LCVSSAMCAAYEARYGVPAEVIYPMRAVNCPNLDAPPPRLGRNEVPFTIAFAGSINSH
                     GYIQALLALQQALEPAGGRLLIYGPMAADAARKVGLDRPHTIVGGLLSAPELMQRLRE
                     EADALFVPMSFDPVDRTNMELAFPSKLADFTAIGLPLVIYGPPYCSAVRWARENYGVG
                     EVVDRVSVSQLSQTISRLAREPPLRVASGKRALEVGREYFAYEKVRQVFNRVVSLS"
BASE COUNT         1187 a         1569 c         1310 g          912 t
ORIGIN      
        1 cctagctggc aacacaacca tcgtgtatgt tggcggttca cgaactctca gccaacggcg
       61 cacgccgcag cgccaggtac gatttcacat cgtgaaacgc gagtggcaag cgatactgcc
      121 gatgcgcgcg cagatgcgca ggaatcatcc ttacgcctgt ccagattccg cggcgccgtc
      181 cgtttcgcag taaccacagc acgcgattaa cacattgcgt gaatccaacc caccaaagcg
      241 aaaccggata acgcagatat gcgagcagcg cgagattcgc gatgctgccc gcggtgacgc
      301 gtggatcgcc gtgccgtgcc aaatccgtgt cgtgaaaaac acgcaaccac gaactcgtca
      361 gaattcggcc gccacgcgaa tggaggcgca acgcgaggtc cacttcttcc atgccgtaag
      421 ctaccggcaa cggcacgtat ccgcccgccg cgagaaaagc gctgcgccga aagacacacg
      481 cccccccgca gaagtcggca gtccactcga tctttcgttc gtcgaatgcg atagtttcac
      541 cgcgatggta gagcgcggcg cagaccacgg aggcatttgg gtgttgcgca aacaactgct
      601 gcgcgcgggc gaagtagtcc gagtcgattg gatacgaatc atcatcaaaa ctcgcgacga
      661 gatcttgcgt caccgctgcg agtagcttgt ttctgccgcc tcctggacca acacgttcgc
      721 gactcaccag cacacgcacg tctggaaacg cgtcgcaaat cgcgccggcg cacgcgaggc
      781 ggttgtcatc aacatgcacg aggatctcgt gaggcgcggg cgcgcaactc tgaatcactg
      841 ttagcgtcgc gagcgtctgc tcgattctct cgtacgcagt gacgatagcc gaaacaccgc
      901 tagccatgat tagtcgccgc taaatcgcgg cacaggtacg cttgcagaat ctccgccagg
      961 gcacgcgcgc cggcggcgtt cggatgaaaa ctgtcgcgat agaaaaggtc ctgtcgccgg
     1021 cccttctgcg caagttgcgc agcccaatcg aagaactccg cgccttcacg ctccgcggcg
     1081 tcgcgcagtc ttgcgttcac agttttgaaa tgttcaggcg aaccgggaaa acgatcctca
     1141 ccgaccggca acaatccgag gacgaccact cgcgcgccca gcgcacgcag cgacttcaac
     1201 aacgacgact catgagtaat caactgctcc gtatccatca gcggcagtcc gttgcgctct
     1261 ttgaggagct ttagacgcgc tttctgcttc aacgcgtcaa cgctcgtttg cttggccttg
     1321 cgccaccacg tagtgctgaa ataacagcgc gggtccatcg ccgcgtaacc ccgccaggac
     1381 gctggaaccc accagggcca cgcgccgccg cggaataccg ccgggcgaat gatgctttcc
     1441 agaccaccgc gaaaagcaac gaccagctcg ggttttagcg ccgcgatcaa aggcaaccac
     1501 gcaaagtcgg aatcgatcgt ctgtccttca caagcaaggt tccagagcca aagatcgcgg
     1561 catgatccgg cacccttcag ttcaccatcg acattcatga accccgcgac gttaaaatcg
     1621 atctgccgaa aagcatccgt ctcaatctgt cttaaacgaa attcatgcac gctgccgttg
     1681 ctcttcgtgc ggagttgcct ggccaactct gaaacgtgac tgaccctctt cgagccgacg
     1741 ccttccgtta ggctgtcacc gagaaacacc caattgcgcg ccttgcaaga catctgctac
     1801 ccgatacgcc gttaacgagc cagaggaatc agcagccgcg caaatgcgcg cagccggccg
     1861 gtcagcggcg cgacaaagcc gcggaaactc aagcgccgga cgcacccgag aaactggctc
     1921 gccgtctcca ccgtgaaata aagcagcacc agaccgaacg aacgcagcaa gctgaagcgc
     1981 attgtgcgcc gcaggataaa gaaacggttg aacagatcgt cttccgcagc catgaacctc
     2041 gtcaccctgc cggaggacgc gtgatgatga gccatgcgcg cggcgggaca gaacatgatc
     2101 gttcctacgc gtcgcagttt caggccaaga tcgatgtctt cgttgatcgt gcagcgatgg
     2161 agaaagaaat ccgaaaaacc gccggccgcg acaaatgccg agcggcgcac aaggctgttg
     2221 cccgagccga gccactcggt ggcgcaaggt tcgcacggca ctgagttgta accgaagcgc
     2281 agcaatggtc caacgacgcg tccctgccac gcgtcttgtt ttaatttcaa gacgtgacgc
     2341 aggtagagct gccacatccg cgtcggggca ggccaggttt ggttgttgaa gtctgcgcta
     2401 acggcaacga cacagtcgtt cgactccagc gactcgatca tgcgagctac gcagtcaggt
     2461 tcgagcacaa cgtcatcgtc caggaacagg aacaattcgc catgagcgac gctgatcgcg
     2521 gcggcgcgct gccgcacggc attaggaggg tgaaccgcta tgcgtttcac aataaaagcg
     2581 gattggcttt tgaaatccgc taaaactctt tcggtttggt cgtcaacact agcgtccgca
     2641 acgatcactt cgtcaactct gtgagtctgc gccgccaccg attcaagcag cgctcgtagc
     2701 gatcgaggcc ggccgatggt gggcacgatg gcgctgatct tcatctcaag tgcgataaaa
     2761 ccaaaaactc gaagacccgc tattcggcga agcccataat cgcttcacct gcgccagatg
     2821 gtccaacgcg agccaatgat tcgcaagcgc aatctgaaac tcacgattaa acgccagaaa
     2881 cgcttccagc aaatactgct cgttcaacgc cagcctccgt ttaagcaacc attccgccgg
     2941 gtaatcatgc gggaagaaga cgtcgtgcac gtgcacccaa acaccgcggc tcagagccgg
     3001 caaaatctcc agaaactcgt agcacacgtc actgccaaac ttcaccgtat gactcgaatc
     3061 gataaacaga atgtcgttcg cttttaacga cgcaaagaaa ctgacctcga tctcctcgac
     3121 gcgtttggtg atcagctcta ctcctaaact cgaaccgttc aatcgctccg gaaagggttc
     3181 gatagacgtg agccgtccag tttgatttgc agccaaagct ttgccggcga tgcgcgtcga
     3241 gtagccgccg ccgatttcaa tgatccgccg gggctttaag cggcgaatca acgagtaata
     3301 gatcactgcg tcgagaccgc tgaagtaatc gttctgaacc tcgctttcgg agagctctcc
     3361 ggcataactc tccagttcgc gcaacaaacg cagttgcgct tcccagcgaa agtcgattcc
     3421 cgaaaactcg cgccgccgct gtatctgttc cggcgtgatc gtgcggaagt cgggcagcgg
     3481 ttcgtagtaa tgaatcggac gaatgtgata gccccaccga tccgaaagct ctggccgctc
     3541 ctggatcagg aacaacgcat gtcgcgccgc acgcgcaggc acacgcctga ataaacccag
     3601 gaccgttttg ttcaccagcg atttcaactc agactcacaa cacgattaaa aacctgccgc
     3661 accttttcgt atgcaaaata ctcccgccca acctcgagcg ctcgctttcc tgacgccaca
     3721 cgtaacggcg gctctctcgc aagacgactg atagtctggc ttaactggga gactgatacc
     3781 cgatccacaa cctctcccac accatagttc tcacgcgccc agcgaaccgc cgaacaatat
     3841 ggtggtccat agattaccaa cggcaatcca atcgccgtga agtctgcgag cttactcgga
     3901 aaggccaatt ccatattagt cctatcaacc ggatcgaacg acatcggcac aaacaacgcg
     3961 tccgcttctt cacgcagccg ctgcatcaac tcaggcgcgc tcaaaagccc gcccacaatc
     4021 gtgtgtggac gatcgagccc aactttccgt gcggcatccg ccgccattgg tccataaatc
     4081 aaaagccggc cgccggcggg ctccaatgcc tgctgcaagg cgagcaacgc ctgaatgtaa
     4141 ccgtgggaat tgatactgcc cgcaaaggca atcgtaaacg gcacctcatt gcgtccaaga
     4201 cgcggcggcg gcgcgtccag gttcggacag ttcaccgcgc gcattggata gataacttcc
     4261 gcgggcacgc cgtaacgcgc ctcatacgcg gcgcacattg ccgacgacac acaaagacgc
     4321 gagcgagccg cgcgataaac atccgcgaac cgggcatcga tccagttgcg aacacccggt
     4381 cgcacgttca ccacgcgcgg ccagtcgtca tggaccatta agtgtagtgg cgcctttcgc
     4441 cgagctgcta aagccgctgc cgctaaccag ccaaagccat gcgcgacagt caacactccc
     4501 tcacacgcga atccattcaa ccgcgccgag atctgcggtc ctcgccgggc agccgcgcgt
     4561 gaataccacg cgaccgcgtg cgcatgaaaa cgcgtgttca accaacgttg tttaccgagc
     4621 gggtgcgaca gatagttgac gtttggtaat cgccttgacg gcatagacgg cgccgcagtt
     4681 tcgattacgg ttagacggtc caccgggtaa tcgaccaaca aacggtgcaa caacgccgag
     4741 ccgtgatacg acgcttctac gggaacatcg ccgacgtaaa gcaaatgcgg caacatgatt
     4801 gctacaaccg gtaaacctgt cgcatccagt cagccgtcag cttcattccc tccgcgagca
     4861 tataggagtt tttgtgatcg aggtcgcgca ctgacttcga aatatcgacg cgtttccact
     4921 ttgtcgtcag gatttccgag tcctggtaat ggaccagacg cggatcggcg cccgtgac
//
LOCUS       DASTEY010000152         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1536126, whole genome shotgun sequence.
ACCESSION   DASTEY010000152 DASTEY010000000
VERSION     DASTEY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536126"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFO72_02680"
     CDS             <1..652
                     /locus_tag="VFO72_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478220.1"
                     /translation="AIKPSSPAGLYVQEKGIDVKDFNSYGARRGNHEVMVRGTFANVR
                     IKNLMVPGVEGGVTIYYPSEERTSIYDAAMRYQEENVPLVIIAGQEYGTGSSRDWAAK
                     GTRLLGVRAVIAQSFERIHRSNLVGMGVLPCQFKDDTSAASLRIDGSERFDLLGIEDG
                     IEPLEEITLVIYRKNGAVEKVPITLRIDTPIEVDYYKHGGILPFVLRQLLAGTTDF"
     gene            668..2140
                     /locus_tag="VFO72_02685"
     CDS             668..2140
                     /locus_tag="VFO72_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9478221.1"
                     /translation="MRTYKLFIDGQWIDSESGKTFQTPNPATGETLAEVAEADKADID
                     KAVSAARRAFEGKWSKMSARDRGRLLYKLSQLIEQHAADLAAIETADNGKPIKESLYV
                     DLPQVVENFEYFAGWATKIEGETIPVPGRMFNYTLREPIGVCGQIIPWNFPLLMAAWK
                     LAPALAAGNTVVLKPAEQTPVGAMELAKLIQEAEFPEGVVNIVPGYGETAGASLAAHP
                     GIDKIAFTGSTEVGKLIAKSAAENLTKVSLELGGKAPNIVFADSDLEQAVAGAMMGIF
                     YNQGQVCCAGSRLFVEESVKEEFVGKLREKAGKIVVGDPANKSTQMGPQVSEEQLNRI
                     KGYVDIARQEGATVVAGGHTPQLEGAFQRGYFFTPTIFDEVKNSMRVAQEEIFGPVVS
                     VITFKDEDDLIKQANDTIYGLSAGIWTRDITRAHRFAREIKAGVVWINTFNMFNAASP
                     FGGYKQSGYGREMGRHALDLYTQVKSVWVDLSGRPIGWFK"
     gene            2280..2633
                     /locus_tag="VFO72_02690"
     CDS             2280..2633
                     /locus_tag="VFO72_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478222.1"
                     /translation="MLALKKTAVLLALLSGTLLLTACPNQTSIAKINADPGRYSGKEV
                     GIAGTVTNSYGAIGHGAYEIDDGTGRIWVATRRGIPSRGARVGAKGYVHNGFSFAGRS
                     FGTVLEETDRRSKSK"
     gene            2678..3169
                     /locus_tag="VFO72_02695"
     CDS             2678..3169
                     /locus_tag="VFO72_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478223.1"
                     /translation="MLVLTSSSMDQDSPHELPHELVAHLDLQFARLIRDLKQLVNSIA
                     PDLLYRRPPNVTIGENLLRSAATLEQTFGGLTANLWDDPFEWTLPETLSTPELVNEYL
                     SEVDAARQRAFKSISTDGELTKYVSGPSSEPQQLFALLLETLAKASDYHGRAVATVKM
                     VFR"
     gene            3231..3779
                     /locus_tag="VFO72_02700"
     CDS             3231..3779
                     /locus_tag="VFO72_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9478224.1"
                     /translation="MFCPQCGQQQVPGVVRFCSRCGFPLDGVMQLLGNGGMLPVYHSP
                     AEPGQISPRRKGVKQGAVLILSGAVIVPILGVLASFSNAAFPQILAAMAAIICFIGGP
                     FRMLYAALFEEGAPPRHFPNYGPPPAAAPQQFGSPRQHTALPPPPARSTSTWRRPNTA
                     ELANPPSVTENTTRLLDKEDRK"
BASE COUNT          863 a         1029 c         1125 g          809 t
ORIGIN      
        1 cgcgatcaag ccttcgtcgc ccgccgggct ctatgtgcaa gagaagggca tcgacgtcaa
       61 agacttcaac agttatggcg cacggcgtgg taatcacgaa gtgatggtgc gtggcacgtt
      121 tgcaaacgtg cgtataaaaa acctgatggt gccgggtgtc gagggcgggg tgacgatcta
      181 ttatcccagc gaagagcgca cgagtatcta cgatgcggcc atgcgctacc aggaggaaaa
      241 cgtgccgctc gtgatcatcg cgggccagga atacggcact ggaagttcgc gcgactgggc
      301 ggcgaaggga acgcggctgc tcggtgtgag ggcagtgatt gcacagagct ttgaacgtat
      361 tcaccgcagt aacctcgtgg gaatgggcgt gctgccctgc cagttcaaag acgatacgag
      421 tgcggcgtcg ttgcgaatcg acggcagcga gcgtttcgat ctgctcggga ttgaagacgg
      481 aatcgaaccg cttgaagaga tcacactcgt gatttaccgc aagaacggcg cagttgagaa
      541 agttccgatc acgctcagaa tcgacacgcc catcgaggtc gattactaca agcacggcgg
      601 aatacttcct tttgttttgc ggcagctcct tgcgggcacg actgactttt aaacagggat
      661 tacacagatg cgcacttata aacttttcat cgacggtcaa tggatcgact cggagtcagg
      721 aaaaacattc caaacgccaa atccggctac tggcgagacg ctggctgaag tcgcggaagc
      781 ggataaagcg gacatcgata aagcggtttc ggcggcgcgg cgcgcgttcg aaggtaaatg
      841 gtccaagatg agtgctcgcg atcgcggccg gttgctttac aaactctcgc agttaatcga
      901 gcagcacgcc gctgatctcg ccgcaatcga aactgccgac aacggcaagc caatcaaaga
      961 atcgctctat gtcgatctgc cgcaagtagt cgagaatttt gagtactttg ccggttgggc
     1021 tacgaagatc gaaggcgaaa cgatccccgt gcccggccgt atgttcaact acactttgcg
     1081 cgaaccgatc ggcgtctgtg gtcagatcat cccgtggaac tttcccttgt taatggccgc
     1141 atggaagttg gcgcccgcac tggccgctgg taacaccgtt gttctgaaac ctgcggaaca
     1201 gacgccggtc ggagccatgg agctggcaaa actgattcag gaagcggagt ttccggaagg
     1261 cgtcgtcaac atcgttcccg gttacggcga aaccgcaggc gcctcgctgg ccgcgcatcc
     1321 gggtatcgac aagatcgcct tcaccggttc gactgaagtg ggaaagctga tcgcgaaatc
     1381 tgccgcagag aacctgacca aggtttcgct ggagcttggc ggtaaagcgc cgaacatcgt
     1441 ctttgctgat tcggatctcg aacaggcagt ggcgggcgcg atgatgggga tcttttacaa
     1501 ccaggggcag gtctgttgcg ccggctcgcg attgttcgtt gaggagtctg tcaaagagga
     1561 gttcgtcggc aagctgaggg agaaagccgg aaaaatcgtt gtcggcgatc cggcgaacaa
     1621 atcgacgcag atgggtccgc aggtttcgga agagcaactg aaccggatca agggttacgt
     1681 cgacatcgcg cggcaggaag gcgcaaccgt tgtcgcgggc ggccacacgc cgcagctcga
     1741 aggtgcgttc caacgtggct acttcttcac gcctacgatc ttcgatgaag tgaagaacag
     1801 catgcgtgtg gcccaagaag agatttttgg accagtggtc tccgtgatta cgttcaaaga
     1861 cgaggacgat ctgattaagc aggcgaacga cacgatctac ggtctgtcgg ctggtatctg
     1921 gacgcgcgac attacgcggg cgcatcgatt cgcgcgcgag atcaaggctg gagtcgtctg
     1981 gatcaatacg ttcaacatgt tcaatgcagc gtctcccttt ggtggttaca agcagtcagg
     2041 ctacgggcgc gagatgggcc ggcacgcgct cgacctctac acgcaggtga aaagtgtttg
     2101 ggtggatctt tccggacggc ccatcggctg gttcaaatga ttgtcgattg ccaattgcca
     2161 atttccaatt tagttacttc tcgttgagcg ggcataaatc ggcaatcgtc aattggcaat
     2221 cggcaatgtt attattactg cctctctccc gcaatgaccc aaacattcaa ggagacgaca
     2281 tgctggcatt gaaaaagacc gcagttttgt tagcgcttct cagtgggaca cttcttttaa
     2341 ccgcctgtcc caaccaaacg agcatcgcaa agatcaacgc cgacccggga cgttatagcg
     2401 ggaaggaagt tggcatcgcc ggcacggtga ccaacagtta cggcgcgatc ggtcacggcg
     2461 cctacgaaat tgatgacgga accggccgta tttgggtagc gaccagacgc ggcattccgt
     2521 cgcgcggcgc acgcgtcgga gcgaagggct atgttcacaa cggcttcagc ttcgccggtc
     2581 gcagttttgg cacagtgctc gaggaaaccg accgccgctc gaaaagtaag tagacaggat
     2641 tacagaactt acaggacttc aagacagcaa aactatcttg ttggtcctga cgtcttcgtc
     2701 aatggaccag gactctcccc atgagctacc gcatgaacta gtagcccatc tcgatctaca
     2761 gttcgctcgc ttaatccgag atctgaaaca actcgtcaat tccatcgcgc cggatctcct
     2821 ctatcgccgt ccgcccaacg tgacaatcgg tgagaacctg cttcgcagcg ctgccaccct
     2881 cgagcagacg tttggcggct tgactgccaa cctctgggac gatccatttg agtggacgtt
     2941 gccggaaaca ctttcgaccc ccgaactcgt caacgaatat ctgtcggaag tcgatgccgc
     3001 gcggcagcgc gcgtttaagt caatctccac cgacggtgag ttaacgaagt atgtttcagg
     3061 cccgtcgagc gaaccgcagc agctcttcgc gttattgctt gagacgttgg ctaaggcgag
     3121 tgattatcat ggccgggcag tggccacggt aaaaatggtt ttccggtgaa ggtggtcaag
     3181 ggagtattat ctagtgcgtc gtaagacttc gttggcgagg aacgatcccc atgttttgtc
     3241 cgcaatgcgg tcagcagcaa gtcccgggcg ttgtccggtt ctgttctcgt tgtggtttcc
     3301 cactcgatgg cgtgatgcag ttgttgggta acggcgggat gctgcctgtt taccactcgc
     3361 ccgccgagcc cggtcaaata tcgccgcggc gcaagggcgt aaaacagggc gcagtcttaa
     3421 tactgtctgg cgcggtgatc gttccgattc tgggagtgct ggcgagtttt tccaacgcgg
     3481 cgtttccgca gattctggcg gcgatggctg cgatcatctg tttcattggc ggtccgttca
     3541 gaatgctcta cgctgcgctt tttgaggaag gcgcgccgcc gcggcacttt ccgaactatg
     3601 gaccaccgcc cgctgctgcg ccgcagcagt ttggatcacc gagacaacac accgcgttac
     3661 caccaccgcc agcgcgttcg acttcaacgt ggcgcaggcc caacaccgcg gagttggcca
     3721 atccgccgag cgttacggaa aacacgacgc gcctgctcga caaagaagat cgtaagtagg
     3781 acagacattc ttgtctgtca gcgacaggca ggaatgcctg tcccac
//
LOCUS       DASTEY010000153         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1387774, whole genome shotgun sequence.
ACCESSION   DASTEY010000153 DASTEY010000000
VERSION     DASTEY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387774"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFO72_02705"
     CDS             complement(<1..630)
                     /locus_tag="VFO72_02705"
                     /inference="COORDINATES: protein motif:HMM:NF018155.4"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9478225.1"
                     /translation="MNRKRGPLALIIIDGWGHSPARDGNAIALADTPFYDELCVKYPQ
                     TLLEAHGLRVGLPAGVMGNSEVGHLNIGSGRVIRMDVSLVDHEIETGEFFRNQVLIKA
                     IEGARDRGKALHLMGLCSDGQVHSSLTHLHALLKLARDLGMQRVYIHCFLDGRDTPPS
                     SAAEYLSALQKRIGEIGCGEIATVVGRYYAMDRDKRWERTQRAYDLLVKG"
     gene            complement(623..1921)
                     /gene="eno"
                     /locus_tag="VFO72_02710"
     CDS             complement(623..1921)
                     /gene="eno"
                     /locus_tag="VFO72_02710"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9478226.1"
                     /translation="MSWIEHVLAREILDSRGNPTVEAEVVLADGEIGRAAVPSGASTG
                     ENEAVELRDEDEKRYGGKGVLKAVHNVNEVIAPALEGLDALDQAEVDRALLELDGTKT
                     KKRLGANALLAVSLATARAAALHLELPLYRYLGGPNSRTLPVPMMNIINGGAHADNNV
                     DFQEFMIVPVGAPNFREALRIGTEVFHALKSVLKKKGYATSVGDEGGFAPNLRSNEEA
                     VETILESINNAGYKAGTDCLLALDPAASEFYDGSSYVFKKSDNRKLTSEGMVDFWKTW
                     CDQYPIISIEDGMAENDWDGWKLLTDKLGSRVQLVGDDLFVTNTEFLQKGIDLGVANS
                     ILIKVNQIGTLTETLDCIELAQRNKRTAIISHRSGETEDPFIADLAVATNAGQIKTGS
                     LSRTDRIAKYNQLLRIEEDLRGASRYLGKKAFHQIEVRFE"
     gene            complement(1890..>2303)
                     /locus_tag="VFO72_02715"
     CDS             complement(1890..>2303)
                     /locus_tag="VFO72_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET9478227.1"
                     /translation="GFVVDEHGGLEGIITLEDLLEEIVGEISDEHDEEVNEQITEIDA
                     RTFVLAGGLAVRDLNRRLKTSVPESETYTTIGGFLMTEAGHVLKPGEVVTYDGLIFHV
                     ERVEKRRVMRVKLELLQTNGEGEPKDELDRARSGA"
BASE COUNT          476 a          686 c          604 g          537 t
ORIGIN      
        1 gcccttgacc aacagatcgt aagcacgctg ggttcgctcc cagcgcttgt cgcggtccat
       61 cgcgtagtaa cggccgacaa ccgtcgcgat ctcgccgcac ccgatctcgc caattctttt
      121 ttgcaacgcg gagagatatt cagcagccga actcggcggc gtgtcgcgcc cgtcgagaaa
      181 gcagtgaata tagacccgct gcattcccag atccctggca agcttcaaca acgcgtgcag
      241 atgagtcaga gatgaatgaa cctgcccgtc tgagcagaga cccatcaaat gcagcgcttt
      301 gccgcgatcg cgcgcgcctt cgatggcttt aatcaacacc tggttacgga aaaactcgcc
      361 agtttcgatc tcgtgatcga ctaacgaaac gtccatgcga atcacgcgac ccgaaccgat
      421 gttcagatgg cccacctcag agtttcccat cacgccggca ggcagaccga cgcgcaagcc
      481 atgtgcttcg agcagtgtct gcgggtactt tacgcagagc tcgtcgtaga agggtgtgtc
      541 ggcgagggcg atggcattgc cgtcgcgagc aggcgaatgg ccccaaccgt cgatgatgat
      601 cagcgcgagc ggcccgcgtt tcctattcaa acctcacctc tatctgatga aatgctttct
      661 ttccgagata gcgagaggcg ccacgcagat cttcttcaat ccgcagcaac tggttgtact
      721 ttgcgatgcg atcggtgcga gagagcgagc ctgttttgat ctggccggcg ttggttgcaa
      781 ccgcgagatc ggcgatgaac ggatcttcag tttcaccgga acggtgcgaa atgatcgccg
      841 tgcgtttgtt tctttgcgcg agctcgatgc agtcgagtgt ttcggtgagc gtgccgatct
      901 ggttgacctt gatcaaaatc gagtttgcga cgccgagatc gattcctttt tgcagaaact
      961 cagtgttggt gacgaagaga tcgtcgccaa ccagttgtac acgactaccg agtttatccg
     1021 tcagcagttt ccacccgtcc caatcgtttt cggccatgcc gtcttcgatg gagatgatcg
     1081 ggtactggtc acaccacgtt ttccagaaat ccaccatccc ttcactggtc agtttacgat
     1141 tgtcgctctt tttgaaaacg taagagctgc cgtcgtaaaa ctcgctggcc gcaggatcga
     1201 gcgccagcaa acaatcagtg ccggctttat aaccggcatt gttgatcgac tcgaggatcg
     1261 tttcgaccgc ttcttcattc gaacgcaggt ttggcgcgaa gcctccttcg tcaccgacac
     1321 tcgttgcata accctttttc ttcagcaccg atttcagcgc gtggaatact tcagtgccga
     1381 tccgcaacgc ttccctgaaa ttcggcgcgc cgacgggaac aatcatgaat tcctggaaat
     1441 cgacgttgtt gtcggcgtgg gcgccgccgt tgatgatgtt catcatcggc actggaagtg
     1501 tgcgcgaatt tggaccaccc agataacggt agagcggcaa ctccaaatgc aaggcagccg
     1561 cgcgcgcggt cgccagcgac actgccagca acgcgttagc gccgagtctt tttttcgttt
     1621 tcgtgccgtc gagctcgagc aacgctcgat cgacctcggc ttgatcgagt gcgtcgagtc
     1681 cttcgagagc cggagcgatt acttcgttga cgttgtgaac tgctttcaac acgcccttgc
     1741 cgccgtagcg cttctcatcc tcgtcacgca gttcgaccgc ttcattctca ccggtcgaag
     1801 ctccagacgg caccgccgcg cgtccgattt cgccgtcggc caacacgact tccgcttcga
     1861 cagtcggatt gccgcgagag tcaagtattt cacgcgccag aacgtgctcg atccagctca
     1921 tccttgggtt ccccttctcc gttagtttgc aaaagctcca gcttaacgcg catcacgcga
     1981 cgcttctcaa cgcgctcgac atggaaaata agtccgtcat acgtcaccac ttcgccgggt
     2041 ttgagtacgt gtcccgcttc agtcatcaaa aagccaccga tggtcgtgta ggtctctgac
     2101 tccggcacag aagttttcaa acgccggttg agatcgcgca ctgccaggcc accggcgagg
     2161 acaaacgtgc gagcgtctat ctcagtaatc tgttcgttta cttcttcgtc gtgttcgtcg
     2221 gaaatctcgc cgacgatctc ctcgagcaaa tcttcgagcg tgatgatgcc ttccaggccg
     2281 ccatgttcgt cgactacgaa acc
//
LOCUS       DASTEY010000154         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1519909, whole genome shotgun sequence.
ACCESSION   DASTEY010000154 DASTEY010000000
VERSION     DASTEY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519909"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VFO72_02720"
     CDS             complement(<1..796)
                     /locus_tag="VFO72_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HET9478228.1"
                     /translation="MPVTITIPAPLRQFAGGQSQIVVEARTAGEALDLLTSTHSALRR
                     HLFNDQNALRNFVNVYLNDEDIRHASGTDTPVKDGDTIMIVPSIAGGAVVESEVGQNL
                     PELSHEEILRYSRHLIMPEVGMQGQRKLKAASVLMIGTGGLGAPVGMYLAAAGVGRIG
                     IVDFDVVEESNLQRQIIHGTKDLGRRKIESARDRLRDINPHIEIETHESRLTSHNAMQ
                     LFRNYDIVVDGTDNFPTRYLVNDACVLSGKPNVYGSIFRFEGQASVF"
     gene            complement(787..1209)
                     /locus_tag="VFO72_02725"
     CDS             complement(787..1209)
                     /locus_tag="VFO72_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9478229.1"
                     /translation="MIRIGEKQLTEIREHGIRDYPYECCGLLLGRYGPDAKVVSETYP
                     ISNAREESAKRNRFLITPEELMNGERYARAHGLEVVGFYHSHPDSPAVPSQYDLEHAW
                     PTYSYIIVSTGADRATDLFSWEQEPDRARFNQEEIQCQ"
     gene            complement(1206..2348)
                     /locus_tag="VFO72_02730"
     CDS             complement(1206..2348)
                     /locus_tag="VFO72_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET9478230.1"
                     /translation="MTKKRHITTQLVHAGERKPPPVGQPVATPIYASATFTYDSMQEI
                     DQVFGGEKAGYIYTRYGNPTIGALEEAIREVEDGATACAYSTGMAAVHAALIACDLKA
                     GSTVLASQDLYGATTSLLQSVFTSFGVKTVTTDFTDLAKVSEKAKEIRPQVLVAETIS
                     NPLLKVCDIKATAQLAHEHGARLIIDNTFASPYLCQPLKLDADMVVHSATKYLGGHAD
                     TMGGIVVSRDEMDRPALFSVMKLVGGVLGVWEAHEILRGLKTLAVRMERQCENARKLA
                     ARLSEHQRVVRVHYPGLGALVSIELRDGTQEAAFRFMDALQLCLRSTSLGDVFTSVLH
                     PATASHRDLLPARRQELGIVDGLVRISVGIESIDDIIADIEQALSA"
     gene            complement(2302..>2687)
                     /locus_tag="VFO72_02735"
     CDS             complement(2302..>2687)
                     /locus_tag="VFO72_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9478231.1"
                     /translation="GTFVGVVRRLRELNRAVRAVAVQPESPLHGLEGLKHLPTAIRPG
                     IYDESLASETLEVSTEDALLMTQRLAREEGLSVGPSSGANVFAAWRLAASLRQPAVVV
                     TVLCDGGERYDDKETPHHDAARTCR"
BASE COUNT          535 a          791 c          768 g          593 t
ORIGIN      
        1 agaacacact cgcctgccct tcgaatctga agatcgaacc gtagacgttt ggcttgccac
       61 tcaacacgca cgcgtcgttc accaggtagc gtgtgggaaa gttgtccgtg ccgtcaacga
      121 caatgtcgta gttgcgaaac agttgcatcg cgttgtggct cgtcaatcgc gactcatgcg
      181 tttcgatctc gatgtgcggg ttgatgtcgc gcaaacgatc gcgcgccgac tcgatcttgc
      241 gccggcctag atctttcgtg ccgtgaatga tctgccgctg caggtttgat tcttcaacca
      301 cgtcgaaatc cacaatgcca atccgtccca cgcccgcggc agcgagatac attccaaccg
      361 gcgcgcccaa accgccggtg ccgatcatca acacgctcgc tgctttgagt tttcgctgcc
      421 cctgcatccc gacctccggc atgatcaagt gacggctgta gcgcaggatc tcttcgtgcg
      481 acaactccgg caagttttgg ccgacttcgg attcgactac agcgccgccg gcaatcgacg
      541 gaacgatcat tatcgtgtcg ccgtctttga ccggcgtgtc ggtgcctgaa gcgtgacgaa
      601 tgtcttcgtc gttcaggtaa acgttgacga agttgcgcaa cgcgttctga tcgttgaaga
      661 gatggcggcg caacgccgag tgtgttgacg tgagcagatc gagcgcctca cccgcagttc
      721 tggcttcgac aacgatttgc gactggccac ccgcgaactg acgcaacggc gcaggaatgg
      781 tgatggttac tggcattgga tctcctcctg gttgaaacgc gcgcgatcgg gctcctgttc
      841 ccacgagaac aggtccgtag cgcgatcggc gccggtcgaa acaatgatgt atgagtacgt
      901 gggccaggcg tgctcgaggt cgtattgcga cggcactgcc ggtgaatctg gatgcgaatg
      961 ataaaagccg acgacttcaa ggccgtgcgc gcgcgcgtaa cgctctccgt tcatcaattc
     1021 ctctggcgtg attaaaaatc ggtttcgctt tgcactttcc tcacgcgcat tcgagatggg
     1081 ataagtctcg ctgaccactt tggcatctgg gccataacgg ccgagtaaca gaccgcaaca
     1141 ttcgtacggg taatcgcgga tgccgtgctc gcggatctcg gttaactgtt tctccccgat
     1201 cctgatcatg ctgagagggc ctgttcgatg tcggcaatga tgtcgtcgat actttcgatt
     1261 cccactgaaa tgcgcaccag accgtcaacg atccccagct cttgtctgcg cgcggggagc
     1321 agatcgcggt gcgatgctgt tgccggatgc agcacgctcg taaacacatc cccgagactc
     1381 gtagagcgca ggcacaactg caacgcgtcc ataaaccgaa aggctgcttc ttgcgtgccg
     1441 tcgcgcagct cgatcgatac cagtgcgcca aggcccgggt aatgaactcg aacgacgcgc
     1501 tgatgctcgc tgagacgggc ggcaagtttg cgcgcgttct cacattggcg ttccatacgt
     1561 actgccagcg tcttcagtcc acgcaaaatt tcgtgtgctt cccaaacgcc aagcactccg
     1621 ccgacgagct tcatcacgct gaacagcgcc ggacggtcca tctcatcacg tgagacgact
     1681 attccgccca tcgtgtcggc atgtccgccg aggtatttcg tcgcgctatg gaccaccatg
     1741 tccgcgtcga gtttcaacgg ttggcagaga tacggcgagg caaaggtgtt gtcgatgatc
     1801 aggcgggcgc cgtgctcgtg cgcgagctgc gcggtggctt tgatgtcaca aacttttagc
     1861 aacggattgg agatggtttc ggcgactagc acctgcggcc gaatctcctt cgctttctcg
     1921 cttaccttgg ctaaatcggt aaaatccgtg gtcaccgtct tcacgccaaa tgaggtaaaa
     1981 acggattgca acagactcgt cgtggcgccg tagagatctt gcgaagcaag taccgtcgag
     2041 ccggctttca agtcacaagc gatcaaggcc gcatggaccg cggccatacc ggttgaatat
     2101 gcacacgctg tcgcgccgtc ttcgacttca cgtatcgctt cttcgagcgc accgatggtc
     2161 gggttgccgt aacgcgtgta gatgtagccg gctttctctc cgccaaagac ttgatcgatc
     2221 tcctgcatcg aatcgtaggt aaacgtagcg ctggcgtaga tcggcgtggc gaccggctgg
     2281 cccacgggcg gcggtttccg ttcaccggca tgtacgagct gcgtcgtgat gtggcgtttc
     2341 tttgtcatcg tatcgttcac cgccgtcgca caggactgtg accaccactg caggctggcg
     2401 cagtgacgcg gccagtctcc aggcggcgaa gacgttggcg ccggaactcg ggccaacaga
     2461 taatccttct tcacgggcta atcgctgcgt cattaataac gcatcttcgg tagagacttc
     2521 cagcgtctcc gaggccaatg actcgtcgta gatgcccggc ctgatcgccg ttggcagatg
     2581 tttcaagcct tcgaggccgt gcagaggtga ttcgggttgc acggcaacgg cacgtaccgc
     2641 gcggttcagt tcgcgtaaac gtcgcacgac accgacgaaa gttccgg
//
LOCUS       DASTEY010000155         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1058439, whole genome shotgun sequence.
ACCESSION   DASTEY010000155 DASTEY010000000
VERSION     DASTEY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058439"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFO72_02740"
     CDS             complement(<1..320)
                     /locus_tag="VFO72_02740"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9478232.1"
                     /translation="MQTRTDELAHRAVEAVARESYGRLVALLATRTRDVAGAEDALAD
                     ALVAALTTWPRAGIPKNPQGWLLTAARNRLVDHARHQRVQEHSTPPLKLMTREFDETA
                     DPDR"
     gene            complement(304..687)
                     /locus_tag="VFO72_02745"
     CDS             complement(304..687)
                     /locus_tag="VFO72_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9478233.1"
                     /translation="MLYALLIYEKPEDFARRDSSVESEAYAGAWRAYHRALLDAGIFV
                     GGKPLDAPETATTVRLHERKREIQDGPYSNTKEQLGGFTLLELPSLDAALEWAARCPA
                     ASYGAVEVRPIAVELEPRVTVANSN"
     gene            complement(825..1964)
                     /locus_tag="VFO72_02750"
     CDS             complement(825..1964)
                     /locus_tag="VFO72_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET9478234.1"
                     /translation="MAKILLLIALCPLLIQAQPKPDKDYLVYVLSEGADKISLIRFGP
                     EGARVERQIDTGDMPVDIDGPHGIVVSPDRQFYYVSIAHGRPFGSVWKYSTKNDKFVG
                     KSVLGYFPATMDISPDGSFLFVVNFNLHGDMTPSSVSVVSTQTMTEVARIQTCTMPHG
                     SRLNTQGTKQYSACMMDDMLVEIDTRSLKAARYFVVTKGQEKGVAEPTHSTHGMKGGA
                     GHGSEAPKPGDNSCSPTWAEPSVDGASVFVACNGSSEIVEVNASSWKLVRRIPARAGV
                     YNLEITHDGQRLIATNKRDQSVSIYDLKSGKELARLATKRKVLHGVVVSPDDRYAFVT
                     VEGVGSEPGTVEVIDLTALKTVATVDVPRAAAGIDFYKTEQPNKP"
     gene            complement(2109..>2421)
                     /locus_tag="VFO72_02755"
     CDS             complement(2109..>2421)
                     /locus_tag="VFO72_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478235.1"
                     /translation="LHRLEGIVIYFGFLLLLFMLLERMDAAEPRRLLLFPVLVYYATT
                     LGIPLLNGSYRQPAFWEHFAFVIILPAILAVFVGAALRGRPRLGFVGWPRRTTPAKTT
                     S"
BASE COUNT          552 a          707 c          642 g          520 t
ORIGIN      
        1 cacctatcgg gatctgccgt ctcatcaaac tcgcgcgtca tgagttttaa aggcggagtg
       61 ctgtgttcct gtacccgctg atgtcgcgca tgatccacga gacggttgcg cgcagcggtt
      121 aagagccagc cttgcggatt cttcggaata ccggcgcgag gccacgtcgt caatgcggcc
      181 accaacgcgt cggccaacgc gtcttcagcg cctgcgacat cgcgcgtgcg cgttgcgagc
      241 agcgccacga ggcgtccgta agattcgcgc gctaccgctt cgacggcgcg gtgcgccagc
      301 tcgtcagttc gagtttgcaa cggtgacgcg tggctccaat tcgacggcaa ttggccgcac
      361 ttccacggca ccgtaggaag cggccggaca tcttgccgcc cattccaatg ctgcatcgag
      421 cgaaggcagc tcaagcagtg taaacccacc caattgctcc ttcgtgttgg aatacggccc
      481 atcctggatt tcgcgcttgc gctcgtgcaa ccgcaccgtg gtcgcggttt cgggtgcgtc
      541 cagtggtttg ccgccgacga agatcccggc gtcgagcaag gcgcggtgat acgcgcgcca
      601 ggcaccggcg tatgcttccg attcgacgga cgaatcacgg cgcgcaaagt cctccggttt
      661 ctcataaatt aataaagcat agagcatctt gttctcctct tcgtggttga accacgctcg
      721 ttagtatgac ggacgacgca cagcgatttc gacaaatgtc gaatcaaagc tttagcttgc
      781 ccgctcaaag cagtagccta aaggctgaac tctaaacagt tcttttaggg cttgttcggt
      841 tgttcagttt tgtagaagtc tatgccggcg gcggctcggg ggacgtcgac ggtggcgacg
      901 gttttgaggg cagtgagatc gatgacttcg accgttccgg gttctgaacc aacaccttcc
      961 accgtcacga acgcataacg atcatcaggc gagacaacca cgccgtgcag tactttccgt
     1021 ttagtcgcta gccgcgccaa ttcctttcca ctcttcagat cataaatcga gactgactga
     1081 tctcgtttgt tcgtcgcgat caatcgttga ccatcatgag tgatctcgag attgtaaacg
     1141 cccgcgcgcg ccggtatgcg cctgacgagt ttccagctac tcgcgttcac ctcaacgatc
     1201 tcactcgagc cattgcacgc gacaaaaacc gaagcgccat cgactgaagg ctcagcccag
     1261 gtaggcgagc acgagttgtc gcccggcttc ggagcctcag agccatgccc ggcgccgcct
     1321 ttcatcccgt gcgttgaatg agtgggctca gctactccct tttcctgccc cttcgtcaca
     1381 acaaaatacc gcgccgcctt caacgaacgc gtatcgatct ccaccagcat atcgtccatc
     1441 atgcaggccg agtactgctt cgttccctgc gtattaagtc gcgaaccatg cggcatcgtg
     1501 caggtttgta tccgcgcgac ttcagtcatt gtttgtgtcg aaaccaccga caccgacgac
     1561 ggagtcatgt cgccgtgcag attgaaattc acaacaaaca gaaagctgcc atccggcgaa
     1621 atatccatcg tcgccggaaa gtaaccgagt acggatttcc caacgaactt atcgttttta
     1681 gtagagtact tccagaccga tccaaacggc cgcccgtgtg caatcgagac gtaataaaac
     1741 tgccggtcag gagaaacgac aataccgtgt ggtccatcaa tatcgacggg catgtcgccg
     1801 gtatcgatct gacgctcgac gcgcgcgcct tcgggtccaa agcgaatcag ggagatcttg
     1861 tccgcgcctt ccgacaagac gtagacgaga tagtctttgt caggttttgg ctgcgcttgt
     1921 attaacaaag ggcagagtgc gatgagtaaa agtattttag ccacagatat cacagattat
     1981 agatacggat tagcgctgat ccaacctcat cgatcactgc gtcccagcgt tccggtgcgg
     2041 gctggcgaaa tagacgcatc gtagaaatac gccgatttct gcacccgcat ggtccaccgc
     2101 tacgcaactt agctcgtagt ttttgcaggg gtggtcctcc gtggccaccc cacgaaaccc
     2161 aaccgcgggc ggccacggag tgccgcccct acaaaaactg caagtatggc tgggagtatg
     2221 atgacaaaag caaagtgttc ccagaacgca ggctgccggt aagagccatt caaaagcgga
     2281 attcccaaag ttgttgcgta gtaaacaagg actgggaata acaacaaccg tcgaggttcg
     2341 gcagcatcca ttcgctcgag cagcatgaac agcaataaca ggaacccgaa ataaatcacg
     2401 attccttcga gccggtgcag t
//
LOCUS       DASTEY010000156         8670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_217796, whole genome shotgun sequence.
ACCESSION   DASTEY010000156 DASTEY010000000
VERSION     DASTEY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217796"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VFO72_02760"
     CDS             complement(<1..1318)
                     /locus_tag="VFO72_02760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9478236.1"
                     /translation="MTADRWDDFLEPPIFEHGLRIIGLEDQLTQDLIGTTINDRYLIE
                     AELKPGGMGRLYLARALKLHNKEVLVKILSRELLSHPYAVTKFKQEAEALSRLDHPNV
                     VGLLDAGRLDNGTPYTVMQLVRGQTLRSQISNEGMDLDRTAAILKQVGAGLDHVHEKK
                     IFHRDLKPENIMLQVLNDGTEWVKVIDFGIAKVKDSQVAPSTIDNVAFGTIAYMSPEQ
                     VRGDDLTAASDVFSLAVVAYEMITGRRPFNPKSAPDLLKLQRARVRVKPVDLRPELPP
                     EAQAVLLRALSFEPKDRYQSAGEFANNLARALTAEKQKASFRNAHALPRRPLMIAGLS
                     TILVCVALAVVVYTFVTQKELPNLRAGPSRAFSYSLTVQRMRGGEEYQEPFQSNGQEP
                     FVSGDRFRLNVSSPESGYLYVFNEGPPQSDHASFKIVYPTNAMNNAS"
     gene            1419..2027
                     /locus_tag="VFO72_02765"
     CDS             1419..2027
                     /locus_tag="VFO72_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HET9478237.1"
                     /translation="MAQSHKITELLEALNNGDPHALDELIPLVDYELRQIAHAYMVRE
                     KPGHTLQTTALVNEALIRLIGKKVTFTSRRQFYALVARRMRQVLIDYARSRVASRRGG
                     RVEHINVDDAVGLSFQESRELLRLDEALTTLSEVNEVQARIVELRYFGGFTLEEIAEI
                     AGVSPSTVEREWRSARDWLKQQMTEQSASAQEKNSKTGSDAA"
     gene            complement(2208..3110)
                     /locus_tag="VFO72_02770"
     CDS             complement(2208..3110)
                     /locus_tag="VFO72_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9478238.1"
                     /translation="MTFKILALIVLLLTQATVAFGQSDRAADAAAIRAHIESIFQAFI
                     DGDEQKIFATHSEDWRGFLEGSRQPIKGIDEYMRANGIDWPKPANAPKSHPYYPAGTT
                     YKIGDFDVHFYGPDLAVASLFGEFLRNSITLRRFRIMDVYAKRKGSWIQVASHTVVDP
                     EWRAEQMSKPMTVSPDIRQRILTAREAVWKAWFANDRAALEKLIPEEVIAINNGSDEW
                     SDRAAVLDGARRFAETGGKLVKLEFPKTEIQVYGNTVLVYTTYVYEIDTNGKRTTASG
                     RATEMFVRRGDELVNVGWHMDSGK"
     gene            3258..4406
                     /locus_tag="VFO72_02775"
     CDS             3258..4406
                     /locus_tag="VFO72_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9478239.1"
                     /translation="MRTPRLRPWMIVSAAWIVPAIFAVINRLAQAHLQDWGPATIRDL
                     LFEFGDWLLYAFLTPGVFAISNRWPLARPHLVRRAALHLLFSILFCVAWATCGQVLRL
                     VLMRIFAPELLQTAMQTGLGQFWRQVGVEWLSWIFTTLPFGVAVYLCVVGIEHATRYF
                     VEAREREVQVARLSEQLSSARFAALQAQLNPHFLFNTLNTIAVLVRDDDRQGAVRIVE
                     HLSELLRRTLTRHRANEVTLGEELELVRQYVAIEHARFSDRLRPEFRIPDSLLSAAVP
                     SFALQHLVENAIRHGIARHPDAGLLIVTADRAGDLLQITVIDDGAGIDPHAAPPAGHG
                     IENTRERLRALYGVQASLEIAPRPEGGTIATLRVPYREMAPEPNDEPG"
     gene            4393..5133
                     /locus_tag="VFO72_02780"
     CDS             4393..5133
                     /locus_tag="VFO72_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478240.1"
                     /translation="MNQAKWKALIADDEPAARRGVRQLLAAFPEFAVAGECRNGAEVL
                     AALNTSKPDVVFLDIQMPGIDGFEVIKRRTVERMPAVVFLTAFDQFAIRAFETEALDY
                     LVKPVSEARFAATMKRLEKRLRSTSRALREETIVVTTARGATVLHLNEIDWIEAAGNY
                     TQLWVGTRSYFLRESLQLLQERVKEHGFIRAHRRALVRLDAVRELRRTRAGALVALLD
                     SGVRVPISRRRSASFTSALRQLGAGVAG"
     gene            complement(5130..6116)
                     /locus_tag="VFO72_02785"
     CDS             complement(5130..6116)
                     /locus_tag="VFO72_02785"
                     /inference="COORDINATES: protein motif:HMM:NF025523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YndJ family protein"
                     /protein_id="HET9478241.1"
                     /translation="MNRVKANVSDMQRLRMFSQRSAALGVVVWVLLSLTVTTDSHETE
                     LIHKIVFFAVLVIVPLGLSLVMAGDQTGSIGLYRAAVLVQPIAAIMTVASFFVEKGTL
                     SAALSVAWLVFSALVALFGVTGLISRGLYPLQESSIEAGLLYLPVAGVWLVVYRLGVQ
                     PFDYGETIILLTVVHFHFAGFAAPIIAGMTGRMLARSAHPPARIYLFTVVAIVAAIPL
                     VAAGITFSPWLGLIGTVLLSLGLLMLAVLTVSRVIPAIPALGTRVLLLIAALSSCTAM
                     VLACLYAYSLVAHIVILRIPTMALSHGLLNAFGFATCSLLAWSTISAKDFPG"
     gene            complement(6343..6672)
                     /locus_tag="VFO72_02790"
     CDS             complement(6343..6672)
                     /locus_tag="VFO72_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET9478242.1"
                     /translation="MHRPEDGYLLRIFVGESDKHGHHPLYEAIVLKAREMGLAGATVT
                     RGIMGFGKHSIIHTAKILRLSEDLPMIVEIADSLENIERFLPTLDEMIKDGLVTLEKV
                     KVIQYKA"
     gene            complement(6682..7056)
                     /gene="crcB"
                     /locus_tag="VFO72_02795"
     CDS             complement(6682..7056)
                     /gene="crcB"
                     /locus_tag="VFO72_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET9478243.1"
                     /translation="MGRLFLIGLAGFVGTLGRYWLSGAIARRYGETFPLGTLVVNLIG
                     CLLAGLLFYILQERFVVNPTARTVIFIGLLGGFTTFSSFGLQTFTLLQDGEFALAALN
                     LTASNFIGLLLVWVGYTLARIL"
     regulatory      complement(7104..7166)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7528..8232)
                     /locus_tag="VFO72_02800"
     CDS             complement(7528..8232)
                     /locus_tag="VFO72_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9478244.1"
                     /translation="MNDRPRILVVDDEPQLIRVLRTGLKSRGYDVRAAADGLAGFEMF
                     TDWHPDLVITDLAMPNVDGLELCRRIRAMSQVPIIVLSAKGEEKIKVEALDIGADDFV
                     TKPFGIDELLARVRASLRRATAPSINEAAQTVLESGDFHVDLEAREVKVRGKEIHLTP
                     KEFELLIYFLKHSGKVLTHRTLLSAIWGGNYVEQNEYLRVFVGNLRKKIEPDAATPRY
                     IVTEPWIGYRFDPGQR"
     gene            complement(8229..>8670)
                     /locus_tag="VFO72_02805"
     CDS             complement(8229..>8670)
                     /locus_tag="VFO72_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478245.1"
                     /translation="KHRVQINSETESHLVRVDANAVSEVIYTLVDNAAKYSPPGSTIR
                     IDAQRGHDGMVTLAVEDQGSGIPVEMRERVFDKFFRATRNGDVSTHQPSGTGMGLAIA
                     KGIVEAHEGKIWIENARNGKGTRIAFTLPIGDEEEPEPGRTSEP"
BASE COUNT         1939 a         2476 c         2338 g         1917 t
ORIGIN      
        1 tcgaagcatt attcatcgcg tttgttggat agacaatttt gaaactggca tggtccgatt
       61 gcggtggtcc ctcgttgaac acgtaaagat aaccagattc gggactggaa acgttgagtc
      121 tgaatctgtc tccacttacg aatggctcct gaccgttcga ctggaagggt tcttgatact
      181 cctctccacc gcgcatcctc tgtaccgtca gcgaataact aaacgcccgt gatggaccag
      241 cgcgcaaatt gggaagctct ttttgcgtaa caaacgtgta gacaactact gccagggcaa
      301 cacaaaccag gatcgtcgac agaccagcta tcattagtgg gcggcgcggg agcgcatgcg
      361 cgtttctaaa cgacgctttc tgtttttcag cggtgagcgc gcgcgccagg ttgttagcaa
      421 actcgccggc gctttgataa cggtccttag gttcaaaaga gagcgcgcgg agtaacaccg
      481 cttgcgcttc cggcggcagt tccggccgaa ggtcaaccgg cttcacccgg acgcgcgccc
      541 tctgaagctt cagcaagtcg ggcgcacttt tcggattgaa aggccgcctg ccggtgatca
      601 tttcgtaggc gacgaccgcc aacgagaaaa catcactggc cgccgtgaga tcgtcgccac
      661 gaacctgctc gggcgacatg taagcgatcg tgccgaacgc cacgttatca atagtgctgg
      721 gcgcaacctg agagtctttt accttggcga tgccgaaatc tatgactttc acccactcgg
      781 tcccgtcatt aagaacctgt agcatgatgt tctcgggttt cagatcacga tgaaagatct
      841 ttttctcgtg aacgtgatcg agtccggcgc ccacctgctt caagatcgcg gcggtacggt
      901 ctaagtccat tccctcgttg ctgatttgcg accgcaaagt ctgcccacgg acaagttgca
      961 tcactgtgta aggcgtgccg ttatcgagtc gacccgcgtc aagtaaaccc acaacattgg
     1021 gatgatcgag acgcgagagg gcttcagcct cttgtttgaa cttcgttacg gcgtatgggt
     1081 gtgacagcaa ctcccgcgac agaatcttta ccaaaacctc cttgttatga agcttcagag
     1141 cgcgagcgag atagagcctg cccatgccac ccggcttaag ctccgcttcg atgagatagc
     1201 ggtcgttgat cgtcgtgccg ataagatcct gtgttagttg gtcttccaaa ccaataatcc
     1261 tgaggccgtg ttcgaagata ggtggttcga gaaaatcatc ccagcggtcg gccgtcatcg
     1321 tggcgaaact cccataagta atgttttaaa gaggacgttc attacatggg ggcgcttttt
     1381 gcgtactcct tttcgaccca ggcacttccg actgtaacat ggcgcaatca cacaagatta
     1441 ctgagctatt ggaagccttg aataacggcg atccccacgc actggacgag ctgattccgc
     1501 tcgtcgatta cgaattgaga cagatcgcgc atgcttacat ggtccgggaa aagcccggcc
     1561 acactctgca aacaaccgct ttagtgaatg aagcgctaat ccggttgatc ggaaaaaagg
     1621 tgacgttcac cagccgcaga caattctatg cactggtggc gaggcgcatg cggcaagtcc
     1681 tgatcgatta tgcgaggtcc cgggtggcgt cgcggagagg aggccgggtt gaacacatca
     1741 acgttgacga cgctgtcggt ttgtcatttc aagagtcgcg cgaactacta aggctggacg
     1801 aggcgctgac gacgctgagc gaagttaatg aagtccaggc cagaatcgtc gagttgcgct
     1861 atttcggcgg tttcactctt gaggagatag cggagatcgc cggcgtctcg ccgtcaaccg
     1921 ttgagcgcga gtggcgctcg gctcgtgact ggctgaagca gcagatgact gaacagtctg
     1981 ccagcgcgca ggaaaaaaac tcaaaaactg gtagcgacgc agcttgaacc ggctccgatt
     2041 tccggacgtg ctcttgcctg ggaccgcagg cgtctcgcct gcacgtcaca acagcgcccg
     2101 attcgtcacc cgtgcaggcg agacgcctgc ggtcccaggc aagagaacgc ccaaatgaac
     2161 tgctgccaca tatgcgaccg tggctcttgg ttgcgattcg agtgaatcta tttgcccgag
     2221 tccatgtgcc aaccgacgtt caccagttcg tcgccgcgcc tgacaaacat ctctgtggcc
     2281 cggccactcg cggtggtgcg ttttccgttg gtatcgatct cgtaaacata ggtcgtgtag
     2341 acaagcactg tattgccgta aacctgaatt tcggttttcg gaaactcgag cttaacgagt
     2401 ttgccgccgg tctcagcgaa gcgcctggca ccgtccagaa ccgctgcgcg atcggaccac
     2461 tcgtctgatc cgttgttgat cgcgatcacc tcttccggaa ttaacttctc cagcgccgcg
     2521 cgatcgtttg cgaaccacgc tttccacacc gcctcacgcg ccgtgagtat tcgttgacga
     2581 atgtccggac tgacggtcat cggcttcgac atctgctccg cacgccactc cggatccacc
     2641 actgtgtgcg acgcgacctg aatccaactt ccttttcgtt tcgcgtaaac gtccatgatc
     2701 cgaaaacgac gaagtgtaat tgagtttcgc aggaactcac cgaacagcga ggccactgcg
     2761 aggtccggac cataaaagtg aacgtcgaaa tcgccgatct tgtacgtcgt tccggccgga
     2821 taatacggat gcgactttgg tgcattcgcc ggtttcggcc aatcgattcc attggcccgc
     2881 atgtactcgt cgatgccttt aattggctgg cgagaacctt ccagaaaacc gcgccagtct
     2941 tcggagtggg tggcgaatat cttctgttcg tcgccgtcga tgaacgcctg aaagatgctt
     3001 tcaatgtgcg cgcgaatggc ggccgcgtct gcagcgcgat ccgactggcc gaacgcaact
     3061 gtagcctgcg tgagcagcaa cacaattaga gcaagaatct tgaaagtcat tgcgagtttc
     3121 ctccggccgc gcatgctatt tgcgctaccc gctcgctgac aactgtttat acgcgaacgg
     3181 cggttctcgc gacgtgaacg gccagaagct ggttgatttg tgttgcgcgt acgctggacc
     3241 aacgctactg tggccgcgtg cgaaccccac gcctgcgtcc ctggatgatc gtgtcggcgg
     3301 cctggatagt gcccgcgatc ttcgccgtca tcaatcggct cgcccaggcg cacctgcagg
     3361 attggggacc cgccacaatt cgcgatctgc tgtttgagtt tggcgactgg cttctctacg
     3421 cgtttctcac ccccggcgtg ttcgcgatat caaaccgctg gccgctggcg cgaccacatc
     3481 tcgtgcgacg cgccgcgcta catctgctgt tttccattct cttctgcgtc gcctgggcga
     3541 cctgcggcca ggtgttgcga ctcgtgctga tgcgcatctt cgcgccggag cttctgcaaa
     3601 ctgcaatgca gactgggttg ggccaattct ggcgacaggt tggtgtcgag tggttgagtt
     3661 ggatattcac cacgttgccg tttggcgtgg ctgtctatct atgcgtcgtg ggaatcgaac
     3721 acgcaacgcg ctacttcgtc gaagcgcgcg agcgagaagt gcaggtcgca cgtctctcgg
     3781 aacaactttc gagtgcgcgc tttgccgctt tgcaggcgca actcaacccg cattttcttt
     3841 tcaacacgct gaacacgatt gccgtacttg ttcgcgacga cgatcgccag ggcgccgtcc
     3901 gtatcgtcga acacttgagc gagctcttgc ggcgcacttt aacgcgccat cgggccaatg
     3961 aagtaacgct cggcgaggaa ttggaactcg tccgccagta cgtcgcgatc gagcacgcac
     4021 gtttctctga tcgcctgcgc ccggagttca gaattcccga ctcgctgctg tctgccgctg
     4081 tgccgagttt tgcgctgcaa catctggtcg aaaatgccat tcgccatggc atcgcacgtc
     4141 atcctgacgc cggtcttctg atcgtcactg cggatcgtgc cggcgatttg ttacagatca
     4201 cggtgatcga cgatggcgcg ggtatcgatc cgcacgcagc accacctgcg ggccacggca
     4261 tcgagaacac gcgcgagcgc ctgcgtgcgc tttatggagt tcaagcgtcg ctcgagatcg
     4321 caccgcgtcc ggaaggcggt acaatcgcga cactgcgcgt gccttatcgc gagatggctc
     4381 cggagccaaa cgatgaacca ggctaagtgg aaagcattga ttgcggatga cgagccggca
     4441 gctcgccgcg gcgtgcgaca actgctcgct gcgtttccgg agtttgccgt agccggagaa
     4501 tgtcgcaatg gcgcagaagt gttggcggcg cttaacactt ccaaaccgga cgttgtcttt
     4561 cttgatattc aaatgcccgg gatcgacggt ttcgaagtga tcaagcgacg aaccgtggag
     4621 cgaatgccgg cggtcgtatt tctaactgcg ttcgatcaat ttgcgattcg ggcattcgaa
     4681 actgaagcac tcgattacct cgtcaagccc gtcagcgaag cgcgttttgc ggcgacgatg
     4741 aaacgtctgg aaaagcgctt acgatcgact tcgcgtgcgc tacgcgaaga gacgatcgtg
     4801 gttacaactg ctcgcggagc aactgtcctt cacttgaacg agattgactg gatcgaagcc
     4861 gcgggaaact acacgcaact gtgggttggt acgcgcagct attttttgcg cgagtcactg
     4921 caattgctgc aagagcgcgt aaaagagcac ggtttcattc gtgcgcaccg ccgcgcgctg
     4981 gttcgtctcg acgcggtgcg cgaacttcgt cgcactcgtg ccggcgcgct cgtggcgctg
     5041 ctcgacagcg gcgtgcgagt gccaatctcg cgccggcgta gcgcgtcatt tacctccgca
     5101 ctgcgccaac tcggagcggg cgttgctggt taaccaggaa agtctttggc gctgatggtg
     5161 gaccaggcga gcagactgca cgttgcaaaa ccaaaagcat tcagcagccc gtgactcagc
     5221 gccattgttg ggatgcgcag aatcacgatg tgagccacaa gactgtacgc gtagagacaa
     5281 gcgagcacca tcgcggtgca gctcgaaagc gccgcaatca gtagcagcac tcgcgttccc
     5341 aaggcaggaa tcgctggtat cacgcggcta acggtgagca ctgccagcat caacaatccg
     5401 agcgagagca gcaccgttcc tatcaggccg agccacggag aaaacgtgat tccggccgcg
     5461 accaacggta tggccgccac gattgccact accgtgaaca gataaattct cgcgggagga
     5521 tgtgctgacc ttgccagcat tctgccggtc attccggcaa tgatgggcgc ggcaaagccg
     5581 gcgaaatgaa agtggaccac cgtcaacagg atgatcgttt cgccatagtc aaacggctgc
     5641 acgccgagcc gatagaccac cagccacaca ccggcaacgg gcagatacaa caagcctgct
     5701 tcaatactcg actcctgcag cggataaagt ccgcgcgaga tcaatccggt tacgccaaac
     5761 aacgccacca gcgcactaaa aaccagccac gctacggaga gcgccgccga caacgtgccc
     5821 ttctctacga aaaaagacgc gacagtcatg atcgcagcaa tcggctggac caacactgcc
     5881 gcgcgatata aacctatgct gccggtttgg tcgcccgcca tcaccagcga aagccccaac
     5941 ggcacgatca ccaagacagc aaagaacacg atcttatgga tcaactccgt ctcatgtgaa
     6001 tccgtcgtca ccgtaagtga aagcaacacc catacaacaa cgcccagcgc agcgcttcgc
     6061 tgtgaaaaca tacgaagccg ctgcatatct gagacattgg ctttcactct gttcattcag
     6121 aatctatgca cgaatcggaa tgaggtggag cggttatgcg tcacgatgat ctcctcgtat
     6181 cacaaactga ctaccggatg aagtctctcg aatgaagtaa acctgtttcc tgggaccgca
     6241 ggcgtctcgc ctgcatctca cggcagcgcc cgattcgtca ccgtgcaggc gagacgcctg
     6301 cggtcaaagc agaaacggcg ccccaagttc caaaccacct cactatgctt tgtattgaat
     6361 tactttcacc ttttccaacg ttactaatcc atccttgatc atctcgtcta acgtgggcag
     6421 gaaccgctca atattctcca ggctatccgc aatctcaacg atcatcggca ggtcttccga
     6481 aagccgcagg atcttcgccg tgtgaatgat gctgtgcttt ccgaaaccca ttattccccg
     6541 tgtaactgtt gctccggcaa gccccatctc tctcgccttc aagacgatcg cttcgtaaag
     6601 tgggtgatgg ccgtgcttgt cgctttctcc gacaaagatg cgcaagagat atccatcttc
     6661 aggtctgtgc atcgcctctc ctcatagaat cctggctaac gtatatccaa cccacaccaa
     6721 tagcaggcca atgaaatttg aagccgttaa gttgagagcc gcgagggcga actctccgtc
     6781 ctgtaatagt gtaaaggttt gtaaaccgaa cgatgagaat gtcgtgaatc cgccgagaag
     6841 ccctatgaag attactgttc gggctgttgg gttcaccacg aatcgttcct gcaggatgta
     6901 gaacagtagg cctgcaagaa gacacccaat caggttaacg accagcgttc ccagcggaaa
     6961 cgtctcacca taacgtcgcg caatcgcgcc cgacagccag tagcggccaa gggtaccaac
     7021 gaaacccgcc aaaccgatta gaaatagtct gcccatcttt ctcctcaaag caaaactcct
     7081 aaccactaca cgcgtcttac gtgcaatggt taggagtcat caacccttcg ggtggttata
     7141 tggcgaactc catcgccgcg gctattctac tcgacaggat tgaagcgggg ccaggggtta
     7201 ctgaggaaga gaatgacgtg acgagagcaa gctcgcggag cgctgagagg ttcccgttgc
     7261 ggaagaagtt agtgagcgtc cttttcgttc acgccgcagt gcgcgccaaa cgatcaactg
     7321 agcaatacaa agcgagccaa aaaggcgaag atcttctgcg acgatgagag ccgcgttggt
     7381 ccaggtccgt tgcgccactt ttattttggt ttttctcata cggagtacgc tgatagaagc
     7441 ctatgcccgt tcgcataaaa gctgcgtaaa ggttttataa gaggttcgta aagacgcgga
     7501 ttctgtgtga attgaaagga cagggaatca ccgctggccc ggatcgaagc gataaccgat
     7561 ccatggttca gtgactatgt aacggggcgt ggcagcatcc ggctcgatct tttttcgcag
     7621 gttgccgacg aagacgcgaa ggtactcgtt ttgctcaaca tagtttccgc cccagatcgc
     7681 ggagagcaat gttcgatgag tcaaaacctt gcctgaatgc ttgaggaaat agatcaacag
     7741 ctcaaactcc ttgggtgtca ggtgtatttc tttaccgcgg accttaactt cacgggcttc
     7801 gagatcaacg tgaaagtctc ccgactccag cacagtttga gcggcttcat tgattgacgg
     7861 tgcggtagca cgacgtagcg aagcacgaac tcgcgcgagt aattcgtcaa ttccgaacgg
     7921 cttggtaaca aaatcgtcgg cgccgatatc aagcgcctcg acctttattt tctcttcacc
     7981 tttcgcggaa agaacaatga tgggcacctg tgacatggcg cgtattcgcc ggcataactc
     8041 cagcccatca acgtttggca tcgcaagatc tgtaattaca agatccggat gccagtcagt
     8101 aaacatctca aatccggcca ggccgtcggc ggccgcgcgt acatcgtagc cgcgagattt
     8161 gagcccagtg cgcagcactc gtatcaattg cggctcgtcg tcgacaacta aaatccgcgg
     8221 tcggtcgttc atggctcaga agtgcgtccc ggttccggct cttcttcgtc tccaatcggc
     8281 aaagtaaaag cgatgcgtgt ccccttgccg tttctggcgt tttcgatcca gatttttccc
     8341 tcgtgtgctt ccacaattcc tttggcgatg gctagtccca tacccgtccc ggaaggctgg
     8401 tgcgtactga cgtcaccatt acgagttgcg cgaaagaatt tatcaaacac tcgttcacgc
     8461 atctcgacgg ggatgccgct tccttggtcc tctactgcca gggtaaccat gccgtcgtgg
     8521 ccacgttgcg cgtcgatcct gatggtcgaa cccggcggcg aatacttggc ggcattgtca
     8581 acgagtgtat aaatcacttc gctaacagca ttcgcatcaa cgcgcaccaa atgtgattct
     8641 gtttcgctgt taatctgcac tcgatgcttt
//
LOCUS       DASTEY010000157         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1173393, whole genome shotgun sequence.
ACCESSION   DASTEY010000157 DASTEY010000000
VERSION     DASTEY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173393"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFO72_02810"
     CDS             <1..1081
                     /locus_tag="VFO72_02810"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET9478246.1"
                     /translation="HKDPLVLNRRAAESDLLIYVNINLVPMDGGHKSVAVGLCDYESL
                     RMHHDPQTIRDSDSYMDPARSALNHKVVQLGKIVDKELNVFHIETAINNRMYGADMDF
                     LLRNEDDFSAFDRMKFEAMRYTMSKLPRPARRKLLHSRPAQYEMIACHAGKTEPVHEK
                     IVAKGFEQYAIPIRGQADILITGIPDISPYNVYSILNPLLVQVMALGYHFNFYRNKPL
                     LKKGGVLILHHPCYDDFDHQFHPSYIEFFHRLLPETRDAFTLREKYEREFANNPSYVE
                     MYRRGNAYHGSHPFFMWYWGENGRQHVGRVIAAGAENAHVPGILGWERADNLTEAIAM
                     ARTYMGNSAEITMLHQPMIGVADME"
     gene            1090..>3145
                     /locus_tag="VFO72_02815"
     CDS             1090..>3145
                     /locus_tag="VFO72_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9478247.1"
                     /translation="MKLSPTEIYKERRIFLIGSTGFLGKVTLSMLLHRFPNVGKIYLT
                     VRARSHEESETRFWNNIITAPPFDPLRERYGSALEGFIRDKVVVKGGDIADTNFGYSE
                     EEAQAIADDIDIIINSAGNVTFNPTLESGLRTNVVGTQNVIAFAKRMKRPALVHVSTC
                     FVAGNRSGAVWESDDVLGYFPRRHEMPGVEFSVDQEIADCAKLADRVRDEARDAMMTA
                     KFRELARKRLNEEGRDVDDPDALGLAVARERKVWTRTRLTDLGVERAAYWGWPNIYTY
                     TKSLGEQLVAAETAIVRSIVRPSIVESALSYPFPGWNEGFTTTAPIIFLIRKGQTQIP
                     ADPKLILDITPVDQVASVMLAVSAQACVEEPQLVYQAATGDSNPNNMERIIGLVGLDK
                     RKYFGDKDTGVKILNRLAARMEPRPVTTEKFEKTSVPMFNSTAKKASSLLERVRPRWG
                     GGRVAEVIDLMKDKVERVEELTRETTEAFDMFRPFTVENAYVFRSENVRSLFARISED
                     ERSLLTWNPERFDWYDYWLNIHLPGLTKWVFPTLEDEMRAQPKRIYTYRDLLELFETS
                     TKRHATRVAMRIERDGRKEQYTYADLRELATRVAGFLASQGIKPGDRVMLVSSNAPEW
                     GMTYLGVLKAGATCIPCDPESSTSEILNFARAGNAAGIIVSEAALDEHQDLRERLEQD
                     GLA"
BASE COUNT          717 a          899 c          902 g          627 t
ORIGIN      
        1 gcacaaagat ccgctggttt tgaatcgtcg cgccgccgaa agcgatctgt tgatctacgt
       61 caacatcaat ctggttccga tggacggcgg tcacaagtct gtcgctgttg gactctgcga
      121 ctacgagtcg ctgcgcatgc atcacgatcc gcagacaatt cgcgattccg acagttacat
      181 ggatccggcc cgctcggcgc tgaaccacaa agtcgtgcaa ctgggaaaga ttgtcgacaa
      241 ggagctgaac gtctttcata tcgaaacggc catcaacaat cgcatgtacg gcgcggacat
      301 ggattttctg ctgcgcaacg aggacgactt tagcgctttc gatcgcatga agtttgaagc
      361 catgcgttac acgatgagca aactgccgcg gccggcgcgt cgcaaactgc tccactcgcg
      421 gccggcgcag tacgagatga ttgcctgtca cgccggaaag actgaaccgg tacacgagaa
      481 gattgtcgcg aagggtttcg agcagtatgc gatcccaatc cgcggccagg ctgacattct
      541 catcaccggc attccggata tttcgccgta caacgtctac tcgattctga atcctctgct
      601 ggtccaggtg atggcgctcg gttatcactt caacttttat cgcaacaagc cgctgcttaa
      661 gaaaggcggc gtgctaatac tgcatcatcc gtgttacgac gacttcgatc accagtttca
      721 cccgagttac atcgagtttt tccatcgcct gctgccggaa acgcgcgatg cgtttacgct
      781 gcgcgagaag tacgagcgcg agtttgcgaa caatccgagt tacgtcgaga tgtaccgccg
      841 cggcaacgcg tatcacggct cgcatccgtt tttcatgtgg tactggggag aaaacggccg
      901 gcagcacgtc gggcgcgtga tcgccgcggg cgctgaaaac gcgcacgtac cgggtattct
      961 cggttgggaa cgcgcagaca atttgacgga agcgattgcg atggctcgca cttacatggg
     1021 caactccgcc gagatcacga tgctgcacca gccaatgatc ggcgttgccg atatggaata
     1081 gggattaaaa tgaaactctc gcctactgag atctacaaag agagacggat ttttctgatc
     1141 ggcagcaccg gctttctcgg taaggtgacg ctgagcatgt tgctgcatcg ctttccgaat
     1201 gtcggaaaga tctacctcac agtgcgcgcg cgttcgcacg aagaatcgga gacgcgcttc
     1261 tggaacaaca tcattaccgc gccgccgttc gatccgctgc gcgagcgtta cggttctgcg
     1321 ctcgagggct tcatccgaga caaagtggtc gtcaaaggcg gcgatatcgc ggacacaaac
     1381 tttggctaca gcgaagaaga agcccaggcg atcgccgacg acatcgacat catcatcaac
     1441 tccgccggca acgtcacttt caatccgacg ctcgagagcg gtctgcgcac aaacgtcgta
     1501 ggcacgcaga acgtgatcgc cttcgccaag cgaatgaagc gcccggcgct ggtccacgtt
     1561 tccacctgct tcgtagcggg taatcgttcg ggcgccgtct gggaaagcga cgacgttctc
     1621 ggttatttcc cgcgccgcca tgaaatgccg ggcgtcgagt tttccgttga tcaagagatc
     1681 gccgactgcg cgaagcttgc agatcgtgtg cgtgacgaag cacgcgacgc gatgatgacc
     1741 gcaaagtttc gcgaactcgc ccgcaagcgg ctaaatgaag aaggacggga cgtcgatgat
     1801 cccgatgcac tcggactcgc cgtcgcccgc gaacgaaagg tctggacgcg aacgcgtttg
     1861 actgatctcg gtgtcgaacg tgccgcctac tggggttggc ccaacatcta cacctacacc
     1921 aaatcgctcg gcgaacaact ggtcgcagca gaaactgcga ttgtccgcag catcgtaagg
     1981 cccagcattg tggaatcggc tttatcgtat ccgttcccgg gttggaacga aggcttcacg
     2041 acgaccgcgc caatcatctt tctcattcgc aaaggccaaa cacaaattcc ggcagatccc
     2101 aagctgattc tcgacatcac gccggtggac caggtcgcgt cggtaatgct cgcagtctca
     2161 gcgcaggcgt gcgtcgaaga accgcagctt gtgtaccagg cagcgaccgg cgattcgaac
     2221 cctaacaaca tggagcggat catcggctta gtcggactcg ataagcgcaa gtacttcgga
     2281 gacaaagata cgggcgtcaa gattctgaat cgtctcgccg ctcgcatgga gccgcggccc
     2341 gtcacgacag agaagttcga gaagacgtcg gttcccatgt tcaactccac cgcgaagaaa
     2401 gcctcctcgt tgcttgaacg cgtgcggccg cgctggggcg gcggacgtgt cgccgaagtg
     2461 atcgatttga tgaaagacaa ggtcgaacga gtcgaagaac tcacgcgcga aacgactgaa
     2521 gcgtttgaca tgttccggcc attcacggtt gaaaacgcct atgtgtttcg ttctgaaaac
     2581 gtgcggtcgc tcttcgcacg gatcagtgaa gacgagcgat cgctgctcac ttggaacccg
     2641 gaacggtttg attggtatga ctactggctc aacatccact tgccgggcct gacgaagtgg
     2701 gtgtttccga cactcgaaga cgaaatgcgc gcgcagccga aacgcatcta cacctatcga
     2761 gacctgctcg agttgttcga aacctcgacg aaacgtcacg cgacgcgcgt ggcgatgcgc
     2821 atcgagcgcg atggtcggaa agagcagtac acctatgccg atctgcgcga actcgcaacg
     2881 cgtgtcgcag gttttcttgc cagccagggt atcaagccgg gcgatcgcgt gatgctcgtg
     2941 agcagcaacg cgccggagtg gggcatgact tacttgggcg tgctcaaagc aggcgcaacc
     3001 tgcatcccgt gcgatccgga gagttcaacc agcgagattc tgaacttcgc acgagccgga
     3061 aacgccgccg ggattatcgt gagcgaggcc gccctcgacg agcatcagga tttacgcgag
     3121 cgactcgaac aggacggtct ggcgc
//
LOCUS       DASTEY010000158         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_118870, whole genome shotgun sequence.
ACCESSION   DASTEY010000158 DASTEY010000000
VERSION     DASTEY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118870"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFO72_02820"
     CDS             complement(<1..594)
                     /locus_tag="VFO72_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HET9478248.1"
                     /translation="MRVTPSKPPPQNRTYASRGTIDPLTFKTLIALGIIVVATIHGYG
                     AAWLAIWMLFHPYKSVKLLGVTVWPQGMIPRHREKLAQSIGNAVGNELVSQQTVFDAL
                     FETDFFQRKIEDFVGVYTSELLARVYPSFIDALPSQARAPVLDTISALQYRLAEYITE
                     MLKSEETAEAIEGFVDRQVDDLAERRVSDVVSAESMDK"
     gene            complement(557..1969)
                     /locus_tag="VFO72_02825"
     CDS             complement(557..1969)
                     /locus_tag="VFO72_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9478249.1"
                     /translation="MNTDMFFKQFYLGCLAHASYLIGSDGEAAVVDPQRDVDEYIAEA
                     ESQGLQIKYVIETHLHADFVSGHQELAARTGAEIIFGEKAQAMFAHRAVRDGEEIRLG
                     KVVLRFMETPGHTPEGICILVTDTESSDQPQKILTGDTLFIGDVGRPDLAGGKGYTPQ
                     AMAEMMYDSLHGKILKLPDEVEVYPAHGAGSMCGRNMSKETWSTIGEQRRFNYALQPM
                     TKEQFVAMMTVDLPEAPAYFPRDAEINRSGARGLSELSPPAALSPQEVSELREQGHVL
                     LDVRSAADFGAGHVPGSLNIGLGGQFAMWAGSLLPLNAAIVVIADTSAQVDESVVRLA
                     RVGIENVKGYLEGGVENWRRAGLPLDSIPQVSVNELKEKLATTEDLQVVDVRRPAEYG
                     NGHVPRALNAPLATLDRIAGQLPFAKDKPTAVICAGGYRSSAAASLLEKFGFTNLLNV
                     SGGTGAWIDAGYPVETPTTK"
     gene            complement(1966..>2493)
                     /locus_tag="VFO72_02830"
     CDS             complement(1966..>2493)
                     /locus_tag="VFO72_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478250.1"
                     /translation="RVETEIGYYVVPAWVTEGIRPGIVACSHHMGRWKLNDEGQRQLM
                     ATVKLEQNGTEWRLKREKGIAPYESSDADTLRIWWKDAGVHQNMTFPVHPDPISGMHC
                     WHQVVRVRAANAGDQPGDIFVDTEKSREVYKKWLTMTRPADKFSPDRTRRPYWMLRPL
                     KPAREFYKLATDFHG"
BASE COUNT          528 a          747 c          668 g          550 t
ORIGIN      
        1 cttatccatc gactcagcac tgactacatc gctcacacga cgttctgcca aatcgtctac
       61 ttgcctatcg acgaaccctt caattgcttc agcggtctct tcgcttttca acatctcagt
      121 gatgtactcg gctagtcgat actgaagcgc ggagattgta tcgagcacag gcgcgcgtgc
      181 ctgcgacggc aacgcgtcga tgaaagatgg atagacgcgc gcgagcagtt cgctggtgta
      241 cacaccgaca aaatcttcga tcttgcgttg gaagaaatca gtttcaaaaa gcgcgtcgaa
      301 gaccgtttgt tgcgaaacga gttcattccc aacggcatta ccaatagatt gcgccaactt
      361 ttcgcggtgg cgcgggatca taccctgcgg ccagacggtg acgcccaaca gtttcactga
      421 cttgtacgga tgaaacagca tccagatggc gagccacgcc gcaccgtaac cgtgaatggt
      481 tgctacgacg atgatgccaa gtgcgattag ggttttgaac gttagcggat cgatggttcc
      541 cctcgaagcg taagttctat tttgtggtgg gggtttcgac ggggtaaccc gcatcgatcc
      601 aggcgccggt gccgcctgag acgttcagca ggttggtaaa cccaaacttc tccagcaaac
      661 tcgcggcggc gctcgaacga tagccaccgg cacagatcac ggcggttggt ttgtcttttg
      721 cgaagggcaa ctgtccggcg atgcgatcga gcgttgccag cggcgcattg agtgcgcgcg
      781 gtacgtgacc gttgccatac tcagccggcc ggcgtacatc gacgacttgc aagtcctcgg
      841 tcgtagcgag tttctctttc aactcgttca cactgacttg cgggatcgag tcgagcggca
      901 ggcccgcgcg gcgccagttt tccactccac cttccaaata gcctttcacg ttctcgatgc
      961 cgacacgcgc aagtcggacc accgactcgt cgacctgagc agatgtatcg gcaatcacaa
     1021 cgatcgcagc gttcaacgga agcaggctac cggcccacat cgcaaactgt ccgccgagtc
     1081 cgatgttcaa ggaaccgggc acatgacccg cgccgaagtc cgcagcggaa cgcacatcga
     1141 gtaacacgtg gccctgttcg cgcaactcgg aaacctcttg cggcgataac gccgccggcg
     1201 gcgacaactc agaaagaccg cgagcgcccg agcgattgat ctctgcatct cgcgggaagt
     1261 acgcaggcgc ctccggaaga tccacagtca tcatggcgac aaactgttct ttagtcatcg
     1321 gctgcaacgc gtaattaaac ctgcgttgct cgccgatcgt tgaccatgtt tctttcgaca
     1381 tgtttcgccc acacatggaa cccgcgccgt gcgccgggta aacctcgact tcatccggca
     1441 gtttcaaaat ctttccgtgc agactgtcgt acatcatttc ggccatcgct tgcggcgtgt
     1501 agcctttacc gcccgcaaga tctggccggc caacgtcgcc gatgaaaagc gtgtcaccgg
     1561 tgagaatctt ctgcggttga tcggaagatt ctgtgtctgt caccagaatg cagatgcctt
     1621 caggcgtgtg acccggagtt tccatgaacc gcagcaccac tttgcccaat cgaatctctt
     1681 cgccatcccg caccgcacga tgcgcgaaca tggcctgggc cttctcgccg aagatgatct
     1741 cggcgccagt gcgcgcggca agttcctggt gaccactgac gaagtcggcg tgaaggtggg
     1801 tttcgatgac gtacttgatc tgtaatccct gtgattcagc ttcggcgatg tactcgtcga
     1861 cgtcgcgttg cggatcgacg accgcagcct cgccatccga accgatcagg taagacgcgt
     1921 gggccaaaca gcctaaataa aactgcttga aaaacatgtc tgtgttcatc cgtgaaaatc
     1981 ggtggccagc ttataaaact cgcgtgcggg tttcaacggt cgcagcatcc agtacgggcg
     2041 acgcgttcgg tcgggcgaaa acttatcggc cggacgcgtc atcgtcagcc actttttgta
     2101 aacctcgcgc gacttttccg tatcgacaaa aatgtcgccc ggctgatccc ctgcattcgc
     2161 ggcgcgcaca cggaccacct ggtgccagca gtgcattccc gaaatcggat cgggatgaac
     2221 cggaaatgtc atgttctgat ggacgccggc atctttccac cagatacgta aggtgtcggc
     2281 atcgctcgac tcgtatggcg cgattccttt ctctcgtttc aaacgccact ctgttccgtt
     2341 ctgttccaac ttcaccgttg ccatcaactg tcgctggcct tcgtcgttta gcttccagcg
     2401 gcccatgtgg tgactgcagg ccacgatgcc gggtcgaatt ccttcagtga cccacgcggg
     2461 cacgacgtag taaccaatct cggtttcaac tcg
//
LOCUS       DASTEY010000159         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_415666, whole genome shotgun sequence.
ACCESSION   DASTEY010000159 DASTEY010000000
VERSION     DASTEY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415666"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VFO72_02835"
     CDS             <1..771
                     /locus_tag="VFO72_02835"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HET9478251.1"
                     /translation="RRVWEEFLVKIAREVEAAMQREMFTPPGGPTYIPREYIVYLSPD
                     DDKDWQGDKRRGLEQGLFHVLSERARELSGQTQLAVKSFAVELRVDGTLNKGEFRVQA
                     VWDETESGHTMVTPRVDPARTAPAFSPHDTIVEKASDGSESEQTVVRPKATALYSIEV
                     WRDGVREAVVPITKNEITIGRGSRSITVDLPVKGDPEVSRVHAVLSRDNEGRYWLVAK
                     GRNPTMVNGRELPREEPTEVVPDQKIAICNFVLRIQPR"
     gene            complement(734..1993)
                     /locus_tag="VFO72_02840"
     CDS             complement(734..1993)
                     /locus_tag="VFO72_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9478252.1"
                     /translation="MEPSTQKLIHTFGALADLGQEIATRGDFTEMVRTSMHLLLGTLA
                     LRRGAVAECLVTGEIRSVAIWGVGEQIFTDLFITEAEKQQFLTAGTSDLLTGNTETTI
                     CFVNRHRDRLGQQQIELVLPMIMRDEVTGLVLLGRKASGEEFSPDDFDTIRAMVRHIG
                     VGIHTHRLLEQVAQRAEENRRLYEQLRATYSETVRAFAAAIDIKDKYTQGHSERVGKY
                     CEIIAREMGWREEEVEGIQIAGYLHDIGKLVVDRDIINAPHNINAKESSELSRHPTAG
                     YEILAQINHPYADIPLMAKYHHERVDGRGYPEGLKDEQIPLGAKIVTLADSFDAMTTD
                     RPYRRRRSFEDVVRDLRENSGKQFDPKVVAAFARAILKEVDGVTKNRRIMKVLGKDYL
                     ADENVPTLLSDLIADLTAVGSAAQSYK"
BASE COUNT          440 a          599 c          632 g          474 t
ORIGIN      
        1 cggcgcgttt gggaagagtt tctggtgaag atcgcgcgcg aagtcgaagc cgcgatgcag
       61 cgcgagatgt tcaccccacc gggtggccca acctacattc cgcgcgagta catagtttac
      121 ctgagtcccg acgatgacaa agactggcaa ggcgacaagc gtcgcggcct ggaacaggga
      181 ctgtttcacg tgctttcgga acgcgcgcgc gaactgagcg gccagacaca gttggcggtc
      241 aaatcgtttg ccgtcgaatt gcgcgttgat ggaacactca acaagggtga gtttcgcgtg
      301 caggcggttt gggatgaaac tgagagcggg cacacgatgg tgacgccgcg cgtcgatccg
      361 gctcgcacgg ctcccgcctt tagtccacac gacacgattg ttgaaaaagc ttccgacggg
      421 agtgaaagcg aacagacggt ggtgcggccg aaggccacgg cactgtattc gatcgaggtc
      481 tggcgcgacg gcgtacgtga agcagtggtg ccgatcacga agaacgaaat cacgattggt
      541 cgtggctcgc gcagcatcac cgtcgatctg ccggttaaag gcgatccgga agtgagccgc
      601 gttcatgccg tgctttcgcg tgacaacgag ggacgttatt ggctggtggc aaaaggtcgc
      661 aatccaacga tggtcaatgg ccgtgagctg ccgcgtgaag aaccgaccga agtcgtgccg
      721 gatcagaaga tcgctatttg taactttgtg ctgcggatcc aaccgcggtg aggtctgcaa
      781 tcagatcaga caacagcgtg ggcacgttct cgtccgcgag atagtctttg ccgagcacct
      841 tcatgatgcg ccggtttttg gtcacaccgt ccacttcctt caaaatcgca cgcgcaaagg
      901 ccgctacgac tttcggatcg aattgctttc cactgttctc ccgcagatcg cggaccacgt
      961 cttcgaatga acgccgtcgc cggtaaggcc ggtccgtcgt catcgcgtca aaagaatcgg
     1021 cgagtgtcac aatctttgcg cccaacggaa tttgctcgtc cttcaaccct tcaggataac
     1081 cgcgtccatc aacccgttca tgatgatact tcgccatcaa cggtatgtcg gcgtacggat
     1141 gattgatctg cgcgagaatt tcgtagcccg ccgtcggatg acggcttagc tcgctcgact
     1201 ctttcgcgtt gatgttgtga ggcgcgttga tgatgtcgcg gtccacgacg agtttgccga
     1261 tgtcgtgcag atagccggcg atctgaatac cttcgacttc ctcttccctc cagcccattt
     1321 cgcgcgcgat gatctcgcag tactttccga cgcgctctga atgtccctgt gtgtatttat
     1381 ctttaatgtc gatcgcggcg gcgaaagctc gtacggtttc actataagtc gcacgcaact
     1441 gttcgtagag tcttcggttc tcttccgcac gctgggccac ctgttcgagg agacgatgcg
     1501 tgtggatgcc gacgccgatg tggcggacca tcgcgcgaat cgtatcgaag tcgtcagggg
     1561 aaaactcttc accgctggct tttctgccga gcagaaccag gccggttacc tcgtcgcgca
     1621 tgatcatcgg caggaccagc tcgatctgtt gctgccccag tcgatcacga tggcggttga
     1681 cgaagcagat tgttgtttcg gtgttgccgg tgaggaggtc agaagtgccg gccgtaagaa
     1741 actgctgctt ctctgcttcc gtgatgaaga ggtccgtaaa gatctgttcg cctacgcccc
     1801 agatcgccac cgagcgtatt tcgcccgtta cgaggcattc ggccacagcg ccgcgacgca
     1861 aagcgagcgt gccgagcagc aggtgcatgg acgttcgcac catctcggtg aaatcgcccc
     1921 gggtggctat ttcctggccc aggtccgcaa gcgccccgaa agtgtgaatg agtttctggg
     1981 ttgaaggctc catcaaactc aatggactat gggagtcgtt tcgaaaccgg taagcgtgag
     2041 caacgcctcg cgctcatctt tcgcaagcgc gacgtgacga gtcagtccaa gcatctcgaa
     2101 cagttgcacg gtctggttgt tcacttcgga gaagataatc ttcgc
//
LOCUS       DASTEY010000160         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1354977, whole genome shotgun sequence.
ACCESSION   DASTEY010000160 DASTEY010000000
VERSION     DASTEY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354977"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFO72_02845"
     CDS             complement(<1..706)
                     /locus_tag="VFO72_02845"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9478253.1"
                     /translation="MSANQFIIIRDDLPIDPVTIITEGLLIGRLGKCELLLNHPSVSR
                     VQAGIKQIEDDYYLFALRPKNPVILNGKPVEENEALAAGDIIEVGPFHLQIDSADGAL
                     VIRVELRIGMKPSEIDVSDPGLSTDNLVAPTEGKAKKPRAAPIASNKALDIFWDKRIR
                     DAGKMARPSPLYPKSGRRSGKAQFNWVPTSDLMSRWPVAFFTWAVVVVGLISVAAAYW
                     YTSAFSPAPISKAHAAT"
     gene            complement(709..>2358)
                     /locus_tag="VFO72_02850"
     CDS             complement(709..>2358)
                     /locus_tag="VFO72_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9478254.1"
                     /translation="TEVLEIPLDVFSSDPELRKQLVEVFARFSTSGTELPVTIEELPD
                     LNALKAAEEEITTGIIDGTNVLVMDMDLCIRCGNCSLACHKMHGQSRLLRRGIQIERP
                     VSIGKKRLQHALVPQVCMHCADPECMTGCPTGAIFRDPFGHIDIDKTTCIGCCDCATQ
                     CPYDAITMVPRAAPPPTSGFVSTLKRALSFKIDPPITPEASDDPVAIKCNLCEDTPLN
                     PEGVRRKRYSCEENCPTGALVRVDPIEYFSEIGPAQQFVFRDQTHAVGRNIHKSDPLA
                     RAWHIGGAALTILSALAIGWAISRYGYDAVLAGSWLTMRWLTGLVGLVGVAVVMSYPL
                     RKQVYRRRAGALRYWMLAHIYVGAIAGVMLLFHSGTRTGGLLTSLLYIAFDLTVLSGI
                     VGIISYYVAPRIMTRIEGEPLLVEDLEARRKELLSDVRTILEKSDGWLKEEIRERVYP
                     RFLSTGFLWRQIWRREELKALLAQARQEFKDRTTRLTTEEERDLLIAAVEAAVTLRRV
                     DALLALHWLLRAWIPLHILATAVMLALMLVHIGQVVFFSVS"
BASE COUNT          529 a          734 c          620 g          475 t
ORIGIN      
        1 gtgtcgcagc gtgcgctttc gagattggcg cgggcgaaaa tgcactcgtg taccagtaag
       61 cagctgcgac cgaaatcaat cccacaacca ccaccgccca cgtaaaaaac gcaacgggcc
      121 atcgcgacat cagatcgctc gtcggcaccc agttaaactg agccttacca ctgcgcctgc
      181 cgctttttgg ataaaggggc gagggccttg ccatcttccc tgcgtcgcga attcgcttgt
      241 cccagaaaat gtcgagcgcc ttgttgcttg ctatcggcgc ggcgcgaggc ttctttgcct
      301 tgccttcggt gggcgcaacc agattgtcag tgctcaaacc cggatcgctg acgtcgatct
      361 cggaaggctt catgccgatc cgcagctcga ctcgtatcac cagcgcgccg tcagcagagt
      421 cgatctgcaa gtgaaatgga ccaacttcga tgatgtcgcc ggcggcgagc gcctcgtttt
      481 cttcgaccgg tttgccgttg aggatgacgg ggttcttagg ccgtaacgca aagaggtaat
      541 agtcgtcttc aatctgcttg attcccgcct gcacgcgcga gacggaaggg tggttcagca
      601 acaactcaca cttccccaga cgcccgatca gcaacccctc ggtaataatc gtcaccgggt
      661 cgatcggcag gtcgtcacga atgatgatga actgattcgc tgacatctct aactaacact
      721 gaagaaaacc acctggccaa tgtggaccag catcagcgcg agcatcactg ccgtcgcaag
      781 aatgtgcagt ggaatccagg cgcgcagcag ccagtgcagt gccaggagcg catcgacacg
      841 acgcaacgtt accgcagcct caaccgccgc gatcaagaga tctcgctctt cctcagtcgt
      901 cagccgcgtg gtgcgatcct tgaactcctg tcgtgcctgg gccaacagcg ccttcaactc
      961 ctcgcgcctc cagatctgcc gccacagaaa cccggttgac aaaaaccgtg gatagactcg
     1021 ctccctgatc tcttccttca accacccatc actcttctcg agtatcgttc gaacatcgct
     1081 cagcaactct ttacgccgcg cttcaagatc ctcgaccagc aacggctcgc cttcgatacg
     1141 agtcatgatt cgcggcgcga cgtaatacga aattatgccg acaatgcctg agagcacggt
     1201 aaggtcaaaa gcaatataca aaagactcgt cagcagtccg ccggttcgcg tgcctgaatg
     1261 aaacagcagc atcacgcccg caatagcccc aacataaata tgggccagca tccagtagcg
     1321 caacgcgccc gcgcgtcgac gatacacctg ttttcgcagc ggatacgaca tcacgactgc
     1381 cacaccgacg agtccaacaa gcccggtgag ccagcgcatc gttagccaac tacctgcgag
     1441 gacggcgtcg tatccgtacc tggaaatcgc ccatccgatc gcgagcgccg agaggattgt
     1501 caatgccgcc ccgccaatgt gccacgcacg tgccagcgga tcgctcttgt gaatgttgcg
     1561 acccaccgcg tgagtttgat ctcgaaacac aaactgctgc gccggaccga tctcgctgaa
     1621 gtactcgatc ggatcgacac gcacgagcgc cccggtcgga caattctctt cacacgaata
     1681 tctctttcgt cgcacgcctt ccggattgag cggagtgtct tcacaaagat tgcacttgat
     1741 cgcgacggga tcgtcggaag cctcgggcgt aatcggcgga tcgatcttga agctcaacgc
     1801 tcttttcagt gtcgaaacga agccggaagt cggcggcggc gccgcgcgcg gcaccatcgt
     1861 gatcgcgtcg tacggacact gcgtcgcgca atcacaacag ccaatgcacg tcgtcttatc
     1921 gatatcgatg tgcccgaatg gatcgcgaaa gatcgcgcct gtgggacagc cagtcatgca
     1981 ttccggatcc gcgcagtgca tgcagacctg aggcaccagt gcgtgttgca atcgcttctt
     2041 tccgatcgac accggccgct caatctgaat acctctgcgc aacagacgcg attgcccgtg
     2101 catcttgtga cacgccaggg aacaattccc gcacctgatg cagagatcca tgtccatgac
     2161 caggacgttc gtgccgtcaa taattccggt cgtgatctct tcttcggctg ccttgagagc
     2221 attgaggtcg ggcaattctt cgatggtgac cggtagctcg gtgccggaag tcgagaatct
     2281 tgcaaagacc tcgaccagtt gcttgcgaag ctccggatcc gaagagaaaa cgtcgagtgg
     2341 tatctcgagc acttcggt
//
LOCUS       DASTEY010000161         4601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1602090, whole genome shotgun sequence.
ACCESSION   DASTEY010000161 DASTEY010000000
VERSION     DASTEY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602090"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFO72_02855"
     CDS             <1..812
                     /locus_tag="VFO72_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9478255.1"
                     /translation="PGFHGKETMKKVGALLSWFFVMASTTMAQTAPDAAELTKLLNEF
                     LAGASRNDPAVHERFWADDLIYTRSAGRRVSKAEILRDVRSAPAPKPDDPKTVYTAED
                     IRIQQYGDTAIVAFRLVATTEADGAKQVQHLLNSGTFVKRDGKWQVVNWQSTRMPRTE
                     EENRADVAAVTAAFREALRNNDVKKLAALTDASFVLTIGSKSEVTRQQFIDALSAITL
                     KRFKSETPRELVSVYGESAVVRGQTSDGKYTMTLVNQSGAWKIAAIHSTEN"
     gene            complement(809..1603)
                     /locus_tag="VFO72_02860"
     CDS             complement(809..1603)
                     /locus_tag="VFO72_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478256.1"
                     /translation="MPVRFRNYSKIFVSLGLILAFCLVEGEAQTRRKRTRRTTRPAAP
                     RPVITNPPIAPPTTTTATNSGDVKIISTADSQSTEPTDPAQAKKPQPDTTTTQPANEE
                     MQQTITNLSKQVNRLADKLSQMQEDDRYQLDMERLTRAEQRAEQLRMQLVDVQTKIAD
                     LEARLEGVEFALRPENIERATATYGSTRPEEAREARRRALESERTRLKSQVKILQTSQ
                     ARLEVSLATADNEVDLLRAKLQQRREQMDAMPPASESKPKKPEPQF"
     gene            complement(1714..2478)
                     /locus_tag="VFO72_02865"
     CDS             complement(1714..2478)
                     /locus_tag="VFO72_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9478257.1"
                     /translation="MNTNGHRSVRQKLAQRALERQLEYQQNKAANLRGREDEVILAMQ
                     RSSGRVRELLEQFQPIADDARVIEVGSGAHGLIFYFGTNRGVGVDPLAVSYRNLFPRW
                     QSRVPTIAAIGEQLPFPDQTFDVVICDNVIDHAESPSRIVTELVRILKPAGLLYFTVN
                     VHHPVYAVAAGVHSGWRALGVPYEIGPFADHTTHLTLSAATELFKKLPLEILSEKSNI
                     AEARARARKQPARHMGDRVKRVFFKNALYQVVGKRI"
     gene            complement(2485..3339)
                     /gene="nadC"
                     /locus_tag="VFO72_02870"
     CDS             complement(2485..3339)
                     /gene="nadC"
                     /locus_tag="VFO72_02870"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET9478258.1"
                     /translation="MNWIDEGALYSQIGLFLREDLGRGDITTQSIIARNTRARARFVA
                     GEKMIVAGLEAAEEVFLTLDSQQQLEAFVSDGEQVEAGKVIARMVGFADVLISAERVA
                     LNLLQRLSGIATLTSQFVAAVAGTGAQIVDSRATTPGLRLLERYAVELGGAYNGRFGL
                     DDGVVVTSNHISILGSVTAAVKSAKERLGYLHKVGVQVASEAELKEAIASGADVIMLD
                     VRSAEDVKRLTDVAKGLSESVMIECSGPITPANVREFAEAGADLIRVEALTNAAPAMN
                     ISFKIQPF"
     gene            complement(3336..>4601)
                     /locus_tag="VFO72_02875"
     CDS             complement(3336..>4601)
                     /locus_tag="VFO72_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET9478259.1"
                     /translation="TLGWPNETDDLKTFYPTSVLVTARDIIFLWVSRMVMMGMKFVGK
                     EPFADVFVTGTILDSQGQRMSKTKMNGVDPLEVFDKFGVDATRLTLAQVGSTDTRWNE
                     KQVESYRNFANKIWNAARFCLLNSEPQTYTDNTDLELHDRWIISRLNRTALEVNAALE
                     AYEFHAAVQTLYHFFWDDFCDWYIELVKADVTAEEDTPRRAATRSRLLGVLEQALRLL
                     HPFMPYITEELWQRLPGRKQLHPAYQGAEPAIMLAAYPEGRAELIDEAAEAEMQAVTD
                     LISRVRNIRSEMNIKPADRVSIILRPGSGKTLDFLLAAKEQIMRIARASEINVGEAPK
                     ASARAVLAGGAELAVPLEGLIDFEQERQRLNREQQKLQAEAAKLEAQLGNPNFVERAP
                     AERVNEVRARIADIAQRSSQLQQTVENLQ"
BASE COUNT          965 a         1332 c         1252 g         1052 t
ORIGIN      
        1 gccccggatt tcacggaaag gaaactatga agaaagttgg agcgttactg agctggtttt
       61 ttgtgatggc gtcaacgacg atggcccaga ccgcaccgga cgcagcggag ttgacgaagt
      121 tgctgaacga gtttttggcg ggtgcatcgc gaaacgatcc ggcagtgcac gagcgtttct
      181 gggctgacga tctaatctac acgcgttcgg caggtcgtcg cgttagcaag gcggagatcc
      241 tgcgcgacgt gcgttcggct ccagcgccaa aaccagacga tccgaaaacg gtctacacag
      301 cggaagatat tcgcatccag cagtacggcg acacagcgat cgttgcgttt cgtctcgtcg
      361 caacgacgga agccgacggc gcgaaacaag tgcagcatct gctcaacagc ggcactttcg
      421 tcaaacgcga tggcaagtgg caagtagtga actggcaatc gacgcgcatg ccgcgcaccg
      481 aggaagagaa tcgagcggac gtcgccgcgg tcaccgctgc ctttcgcgag gctttacgca
      541 acaatgacgt caaaaagtta gctgcgctta ccgatgccag ttttgtgttg acgataggct
      601 caaagagcga ggtcactcgg caacaattca tcgatgcgct gagcgcaata acgttgaaaa
      661 ggttcaaatc agaaacgcca agagaacttg tctcggtata tggtgaaagt gcggtcgttc
      721 gaggtcagac ttcggacggc aagtacacga tgacgttggt taaccaaagt ggcgcctgga
      781 aaatcgcagc aattcacagc acagaaaact agaactgtgg ctcgggtttc tttggcttgg
      841 attcactcgc aggcggcatg gcgtccatct gttcgcgacg ctgctggagc ttcgcacgga
      901 gcaggtccac ttcgttatcg gcagtcgcaa gcgacacttc taaccgcgcc tggctggttt
      961 gtagaatctt cacctgcgat ttcaaccgcg ttctttcact ctcgagtgca cgccgtcgcg
     1021 cttcgcgcgc ttcttccggt cgcgtagaac cataagttgc cgtcgcgcgt tcgatgtttt
     1081 ccggtctcag ggcgaattca acgccttcga gccttgcctc gagatccgca atcttggtct
     1141 gcacgtcaac caactgcatg cgcaactgct cggcacgctg ttcggcgcgc gtcagacgct
     1201 ccatgtcgag ctgatagcgg tcgtcctcct gcatctgact caacttatcc gcaagacgat
     1261 tgacctgttt ggacaggttc gtgatcgtct gctgcatctc ttcgtttgca ggctgcgtgg
     1321 tggttgtgtc aggttggggc ttctttgctt gagccgggtc ggttggttcc gtggactggg
     1381 aatccgccgt gctaatgatc ttcacgtcgc cgctgttggt ggcggttgtc gttgtaggcg
     1441 gcgcgatcgg cggatttgta atgacgggtc tgggcgccgc cggtctcgtt gtccggcgcg
     1501 ttcgcttccg tctagtctgc gcctcgccct caaccaaaca gaaagcgaga atgaggccga
     1561 ggctaacaaa gattttgctg tagtttcgaa agcgaacagg catcgtcacg ctccttcttt
     1621 ccggtctaag ataccccgct atccagatgc catcaagctc cagtcagatg cttggcttcc
     1681 acgtcggatc gggcggttcc gttccaaaat cccttaaatc cttttaccta ctacttgata
     1741 cagggcgttc ttgaagaaaa cacgctttac acgatcgccc atgtgtcgtg ccggctgttt
     1801 gcgtgcacgc gcccgagcct cagcgatatt gctcttttcg ctcagaattt cgagcggcaa
     1861 ctttttgaac agttcagtag cagccgataa cgtcaggtgc gtggtgtgat ccgcaaaagg
     1921 ccctatttca tacggaacac cgagcgcgcg ccaacccgaa tgcacgccgg cggcgaccgc
     1981 ataaaccgga tgatgaacgt tcacggtgaa atacagcaac ccggccggct tcaaaattcg
     2041 caccaattca gtgacaattc gggatggtga ttccgcatgg tcaatcacgt tgtcgcagat
     2101 gaccacgtcg aaggtttgat cgggaaacgg aagctgctcg ccgatggccg cgatcgtcgg
     2161 cacgcggctt tgccagcgcg gaaacagatt gcggtaactg accgccagcg gatcgacacc
     2221 gacccctcgg ttcgtgccga agtaaaagat caaaccgtgc gcgccgctgc cgacctcaat
     2281 cactcgagca tcgtccgcga ttggctgaaa ctgctccaac agctcgcgca cgcgtcctga
     2341 ggatcgctgc atcgcgagaa tcacttcatc ttcgcgtcct ctcaggttcg ccgctttgtt
     2401 ttgttgatac tccaactgcc gctcaagcgc ccgttgggcg agcttctgac gaaccgatct
     2461 gtgtccattc gtgttcatgc gtggctaaaa aggctggatt ttaaaactta tgttcatcgc
     2521 aggcgcagcg ttggttaacg cttccacgcg gatcaaatcg gcgcctgctt ccgcaaactc
     2581 gcgcacgttt gccggcgtaa tcggtcccga acactcgatc atcactgact cggaaagtcc
     2641 ttttgcaaca tcagtaagac gcttaacgtc ttcagcagac ctgacgtcca acatgatcac
     2701 atctgcaccg ctcgcgatcg cttccttcag ttcagcttca ctcgccacct gcacgccaac
     2761 cttgtgcagg tatccgagcc tctctttggc actcttcacc gccgcggtca cgctaccgag
     2821 aatggagatg tggttcgacg ttaccaccac gccatcgtcc aaaccaaagc ggccgttgta
     2881 tgcgccaccg agttcgacgg catacctttc gagcagtcgc aggccgggcg tcgtcgcacg
     2941 cgaatcgaca atctgtgcgc cggtgcctgc gacggcggcc acaaattgac tcgtaagcgt
     3001 cgcaatgcct gagagtcgct gcaacaggtt caacgcgacg cgctccgctg aaatcagcac
     3061 gtcggcaaaa ccgaccatgc gtgcaatcac ttttccagcc tcgacctgtt cgccgtccga
     3121 aacaaacgct tcgagttgtt gttgcgagtc gagtgtcaga aagacttctt ccgctgcctc
     3181 gagtccggcg acgatcatct tctcgcccgc tacgaagcga gcgcgtgcgc gtgtgttacg
     3241 cgcgatgatc gattgtgtgg tgatatcgcc gcgtccgaga tcttcgcgta gaaaaagtcc
     3301 gatttgcgaa tagagcgcgc cttcgtcgat ccagttcatt ggaggttctc gactgtctgc
     3361 tggagttgtg aggaacgttg agcaatgtcc gcaatacgtg ctcgcacttc gttgacgcgt
     3421 tcggccggtg cgcgctcgac gaagtttgga ttgccgagtt gagcttcgag tttggctgct
     3481 tcagcttgca gtttttgttg ttcgcgattg agacgctggc gctcttgttc gaagtcgatc
     3541 aagccttcga gcggcacagc gagttccgcg ccgccggcta gtacggccct tgccgaagcc
     3601 ttaggggcct ctccaacatt tatttccgaa gcgcgagcaa ttcgcatgat ctgctctttg
     3661 gctgccagga gaaagtcaag agtttttcct gaaccgggac ggagaattat ggaaactctg
     3721 tccgcgggtt tgatgttcat ctcagaccga atgtttctga cacgcgaaat aaggtcggtc
     3781 actgcctgca tttccgcctc tgccgcttcg tcaatcagtt ccgcgcggcc ttcaggataa
     3841 gccgccaaca tgatcgccgg ctccgcgcct tgataagccg gatggagttg cttacgcccc
     3901 ggcaaacgct gccacaactc ttcggtgatg taaggcatga acggatgcag cagtcgcaac
     3961 gcttgctcca acacacctaa caaacgcgat cgcgtcgcag cgcgacgcgg cgtgtcctcc
     4021 tcagctgtga cgtctgcttt gacaagttcg atgtaccagt cgcagaagtc gtcccagaag
     4081 aagtgataaa gcgtctgcac ggcagcgtga aactcatacg cctccagcgc cgcattcacc
     4141 tccaacgctg ttctatttaa ccgcgaaatg atccaacgat cgtgcagttc aagatccgtg
     4201 ttatccgtgt aagtctgtgg ctcagaattc aacaggcaga atcgcgcagc gttccagatc
     4261 ttgttggcga aattgcgata cgactcaacc tgtttctcgt tccaacgcgt gtccgtgctg
     4321 cccacctgtg ccagcgtgag tcgcgtcgcg tcgacaccaa acttatcgaa cacttcgagc
     4381 ggatcgacgc cgttcatctt cgtcttcgac atccgctggc cctggctgtc gagaatcgtg
     4441 ccggtcacga aaacgtccgc aaacggctcc ttgcccacga acttcatgcc catcatcacc
     4501 atccgcgaga cccacaaaaa gatgatgtcg cgagccgtaa cgagcaccga ggtcgggtag
     4561 aacgttttta gatcgtccgt ttcgttgggc caaccaagcg t
//
LOCUS       DASTEY010000162         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1371451, whole genome shotgun sequence.
ACCESSION   DASTEY010000162 DASTEY010000000
VERSION     DASTEY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371451"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFO72_02880"
     CDS             complement(<1..290)
                     /locus_tag="VFO72_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9478260.1"
                     /translation="MQNKGLCDQSLTKLAELIRKREVSPAEVVEAHLEQISRLNPALN
                     AIVTLAADVTETSQGPLGGVPITVKDTIETAGLRTTSGSRIRAEYVPERDAPA"
     gene            402..1076
                     /locus_tag="VFO72_02885"
     CDS             402..1076
                     /locus_tag="VFO72_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HET9478261.1"
                     /translation="MAIRLSRTFILRKLHQLSGIFPLGIFLLEHFYTNSKALTGPGDF
                     NSAVDDLQSIPYILFIEIGGIFIPLIYHAVYGLVITWEARPNNLAYPYPRNWFYLIQR
                     ITGIILFFFILFHVLNFRFGMIPGLNTISVAHYPEQAFDIVAGEFRMVPIFIVYMIGI
                     TATVWHFANGIWLFLVDWGITIGERAQRLTGYACIAFGVVLLLVGVNAAVAFIRPGGL
                     LGGIIY"
     gene            1076..2875
                     /gene="sdhA"
                     /locus_tag="VFO72_02890"
     CDS             1076..2875
                     /gene="sdhA"
                     /locus_tag="VFO72_02890"
                     /inference="COORDINATES: protein motif:HMM:NF006392.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET9478262.1"
                     /translation="MASNGLKIAVVGGGLAGLAAAMKIAEAGHAVDLFSVVPVKRSHS
                     VCAQGGINGAVNTKGEGDSTWEHFDDTVYGGDFLANQPPVKAMCEMAPSIIYLFDRMG
                     VPFSRTKEGLLDFRRFGGTKHHRTAFAGASTGQQLLYALDEQVRRYEVNGNVRKFEHW
                     EMMSLVLDDHQVCRGLVAMDLQTLELRAFPADAVIIATGGPGLIFGKSTNSMVCTGSA
                     VSACYQQGAKYANGEFIQVHPTSIPGEDKLRLMSESARGEGGRVWVPRKKGDTRPPSS
                     IPENERWYFLEEKYPAYGNLVPRDIATREIFQVCLQEMGVGGQNQVYLDLTHIPADVL
                     DRKLGAILEIYEMFVGDDPRHVPMRIFPGVHYSMGGLWVDYGQRTNIPGLLAAGECDY
                     SIHGANRLGANSLVSCVFGGFVAAPAAMEFAKNVERNGVDGSGIYDRELKLQQEINDR
                     LIKQSGNENQYLLHQEMGKWMTDNVTVVRYNDKLKATDDKLLELMDRYKRISINDSNL
                     WATMALPHARHLANMLELARVITLGALNRNESRGAHYKPEFPERDDENFMKTTIAEYS
                     GEGPVFSYDPIDVSLIAPRKRDYSKGKKEAAKV"
     gene            2882..>3524
                     /gene="sdhB"
                     /locus_tag="VFO72_02895"
     CDS             2882..>3524
                     /gene="sdhB"
                     /locus_tag="VFO72_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET9478263.1"
                     /translation="MPTTPKTVEIHIRRRKNPDSPQYWEEFEIPYRPNLNIISCLMEI
                     QKNPVNKAGEPTTPPVWHMNCLEQVCGICTMVINGRVRQSCSALIDKLDQPIKLEPMS
                     KFPNVRVLVVDRSRMFEHLKQVHAWIELDGTYDLGAGPRMDQDDVMERYAYSRCMTCG
                     CCLEACPQYEDNQYIGPQAIAQVRLFNMHPTGKMNREERLEGLMQEDGITNCGN"
BASE COUNT          772 a          964 c          966 g          822 t
ORIGIN      
        1 gccggtgcat ctcgttccgg cacgtattcc gctctgattc gtgacccact tgtcgttcga
       61 agtccagcgg tctcgatagt gtctttgacg gtgattggca cgccccctaa agggccttgc
      121 gaagtttcgg tcacgtccgc cgccagtgtc acaatcgcat tcaacgccgg attcaaacgc
      181 gagatctgct ccagatgcgc ctccacaact tccgctggtg acacttccct cttccggatc
      241 agttcggcga gttttgttag cgattggtcg cagagaccct tgttttgcat ttcgtcaact
      301 tctcaatata attcaattgc tctgtgcaaa ctcatttaca acctgcctga ttcaattcga
      361 ctggcgtcga tgttcaagct taacggcaaa ggagtgcatt cttggctatt cgtttgagca
      421 ggaccttcat cctgcgcaag ctgcatcagc tttccggcat tttccctctt ggaatttttc
      481 ttctagaaca cttttacaca aactccaagg cgttgacggg cccgggtgat ttcaacagtg
      541 ccgtcgacga tctacagtcg attccctaca ttctttttat cgagatcggc ggcatcttta
      601 taccgctcat ctatcacgcc gtctacggtc tggtgatcac gtgggaggca cggcctaaca
      661 acctcgcgta tccctatccg cgcaactggt tttacctgat tcaacgcata accggaatca
      721 ttctgttttt cttcatactg tttcacgtgc tcaacttccg gtttggcatg attccgggac
      781 tcaacacgat ctcggtggcc cattatccgg aacaggcttt cgacattgta gccggtgagt
      841 ttcgcatggt gccgatcttt atcgtctaca tgatcggtat caccgccacc gtctggcatt
      901 tcgcgaacgg catctggctg tttctcgtcg actggggcat aactatcgga gagcgcgcac
      961 agcgcctgac tggttatgct tgtatcgctt tcggcgttgt gctgctgctg gtcggcgtta
     1021 acgccgccgt cgcgttcata cgtcccggcg gactgttagg aggcatcatt tattaatggc
     1081 aagtaatggt ctgaagatcg cggttgtcgg tggcgggctc gcgggtcttg ctgccgcgat
     1141 gaagattgcc gaagcgggcc acgcggtcga tctgttttca gtcgtacccg tgaagcgttc
     1201 gcactctgtt tgtgcccagg gcggcatcaa cggcgcagtc aataccaaag gcgaaggcga
     1261 ttcgacgtgg gaacatttcg acgacactgt ctacggcggc gacttcctcg ccaaccaacc
     1321 gccggtcaag gcgatgtgtg aaatggcgcc gtcgatcatc tatctgttcg accgcatggg
     1381 tgttccgttc tcacgcacca aagaagggtt actcgacttc cgtcgtttcg gtggcacgaa
     1441 gcatcaccgc accgcgtttg ccggcgcgtc aaccggacaa caactgctct acgcgctgga
     1501 cgaacaagtg cggcgttacg aagtcaatgg caacgtgcga aagtttgaac actgggaaat
     1561 gatgtcgctg gtgctcgacg atcatcaggt ctgtcgcggt ctcgttgcga tggatcttca
     1621 gacgctcgaa ctgcgagcgt ttcctgccga cgccgtcatc atcgccaccg gtggaccagg
     1681 actcatcttc ggcaagtcga ccaactcaat ggtttgcacc ggtagcgcag tctccgcgtg
     1741 ttatcaacag ggcgcaaagt acgcaaacgg cgagtttatt caggttcacc caacgtcgat
     1801 accgggcgaa gacaagttgc gcctgatgtc ggaatcagcg cgtggtgaag gtggacgtgt
     1861 ttgggtgccg cgaaagaaag gtgatacgag gccgccctcg tccattccgg agaacgaacg
     1921 ttggtacttc ctcgaagaga agtatcctgc ctacggcaac ctcgtgccac gcgacatcgc
     1981 gacgcgcgag atctttcagg tgtgtttgca agaaatgggt gttggcggcc aaaaccaggt
     2041 ctatctcgat ctgacgcaca ttcccgctga cgttctcgat cgaaaattgg gcgccattct
     2101 cgaaatctat gaaatgttcg ttggcgacga tccgcgtcac gtcccgatgc gaatctttcc
     2161 gggtgtgcac tactcaatgg gcgggctgtg ggtcgactac ggtcagcgca ccaacattcc
     2221 cggactgctc gccgccggtg aatgcgacta ctctattcac ggcgcgaatc gtttgggcgc
     2281 aaactcgctt gtgagctgcg tctttggcgg gtttgtagcg gcgcccgccg cgatggaatt
     2341 tgcaaaaaac gtcgagcgaa acggtgtcga cggctcaggt atttacgacc gtgagttgaa
     2401 actccaacag gagatcaacg atcgcctgat caagcaaagt ggtaacgaga accagtatct
     2461 gctgcaccag gagatgggca agtggatgac cgataacgtc accgtggtgc gttacaacga
     2521 caagctaaag gccactgacg ataagttgtt ggagctgatg gatcgttata aacggatctc
     2581 gatcaacgac tcgaacctgt gggccactat ggcgctgccg catgcacgtc atctcgcgaa
     2641 catgctcgaa ctggcgcgcg tgatcacgct cggtgcttta aatcgtaatg agtcgcgcgg
     2701 cgcgcactac aagcctgaat ttcctgagcg tgacgatgag aatttcatga aaacgacgat
     2761 cgccgagtac tcaggcgagg gtcccgtatt ctcatacgac ccgattgacg tctctttgat
     2821 tgcgccgcgt aaacgagact acagcaaagg caagaaagaa gcagcaaagg tctaatcatc
     2881 cgtgccaact acaccgaaaa cagtcgagat acacattcgc cgccgcaaga acccggactc
     2941 gccacagtac tgggaagagt ttgagattcc ctatcgcccg aatctgaaca tcatctcgtg
     3001 tctgatggag attcagaaga acccggtgaa caaggcgggc gaaccgacga cgccgcccgt
     3061 gtggcacatg aactgtctgg aacaggtgtg cgggatctgc acgatggtca tcaacggccg
     3121 cgtgcgccag tcgtgctcgg cgctcattga taaactggac cagccgatca agctcgagcc
     3181 gatgtcgaag tttcccaacg ttcgggttct ggtcgtggat cgttcgcgaa tgtttgaaca
     3241 cctgaagcag gtgcatgcat ggatcgaact cgacggcact tacgatctgg gagctgggcc
     3301 gcgcatggac caggacgacg tgatggagcg ttacgcctat tcacgctgca tgacttgcgg
     3361 ttgttgtttg gaagcctgcc cgcagtatga ggacaaccag tacattggcc cacaggcaat
     3421 cgctcaggtc cgtctcttca acatgcatcc gacagggaaa atgaaccgcg aggaacgttt
     3481 ggaaggactc atgcaggaag acggaattac aaactgcggc aacg
//
LOCUS       DASTEY010000163         3461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1140785, whole genome shotgun sequence.
ACCESSION   DASTEY010000163 DASTEY010000000
VERSION     DASTEY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140785"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2384)
                     /locus_tag="VFO72_02900"
     CDS             complement(<1..2384)
                     /locus_tag="VFO72_02900"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET9478264.1"
                     /translation="MIAEEFGANAEYVQGLLERFRTNPDLVDDSWRTYFNEILGDGEG
                     AARTFNNGAKAAVVESDRTVRAAVKETSAAAAPAPERKPVEARPPENVEVVPIRGPAL
                     KIVENMERSLSVPTATSERRIPVKLLDENRRLINKHLAEADRGKASYTHLTAWAILRA
                     LDVFPQLNDGFFEGEGGVARLKRPEVNLGVAVDLTKKDGTRTLLVPNIKNANSLTFSE
                     FLSAYDDVVQRAREGKLQVTDFQNTTVSLTNPGTIGTVASVPRLMEGQSLIVATGAIE
                     YPAEYQAMAPETLSQLGISKAISISSTYDHRIIQGAESGAFLARIHEFIIGKHEFYDT
                     IFADLGIPHVPFRWNLDRNPFFTQGDQVLEQSIKEARVIELINAYRVRGHLIADTDPL
                     HALPRLYHPEVDIETYGLTIWDLDRVFTTDGLANKQASTLREILDVLQRAYCGKVGIE
                     YRHIQSKEEKTWIREQIRRELVQPEPLPVELKKRLLWKLISAEQFERFLHTKYLGQKR
                     FSLEGCETIIPMLDKLIDGAGERGVQEIVLGMAHRGRLNVLANVIGKLCERIFAAFEG
                     SVHPEFPADEGDVKYHQGATGERTLADGRKVQLTLSPNPSHLEAVDPVVEGMVRAKQD
                     EIMGQRGATREQVMDLALPVLLHGDAAFAGQGIVMETLNLAGLKGYRTGGTIHIIINN
                     QIGFTTGPEAGRSTIYSTDVARMTQLPIFHINGDDPEAAYRVVQIALNYRQEFNKDVV
                     LDVIGFRRLGHNETDEPSYTQPLMYARVKAHPGVRALYAKRLIKEGVVSEEEVN"
     gene            2535..>3461
                     /locus_tag="VFO72_02905"
     CDS             2535..>3461
                     /locus_tag="VFO72_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET9478265.1"
                     /translation="MKKKTNQRNAKKSGANVDRRNFVKALPAFGIAGVAASRLPFDVL
                     AQTPAASPTPTPSPSPSPTPTPRITKEMMRQAEKLIGIELTDAQEAMAVGGVNRNLDS
                     YEAIRKVDVPLDTEPAIAFHPSRAKKELYVQRSKFRFAKVEPPPFKTVEDLAFATVPQ
                     LAELIRTRRITSTELTKMYLGRLKRYGPKLLCVVTLTEELALKQAAAADAELKRGKYR
                     GPLHGIPWGAKDLFATKGIKTTWGAEPYRDQMIDYDATVIERLNEAGAVLVAKLSMGA
                     LAQGARWFAGVTRNPWQPDEAQTGSSGSSAGAA"
BASE COUNT          709 a          995 c          980 g          777 t
ORIGIN      
        1 ttgttaactt cttcctcgct gacgacgcct tccttgatca aacgcttcgc gtacagcgcg
       61 cgcacgcccg gatgtgcctt cacgcgcgca tacataagcg gctgcgtgta gcttggttcg
      121 tcagtttcgt tgtgcccaag ccggcggaaa ccgatcacgt cgagcaccac gtctttgttg
      181 aactcctgac gatagttcag agcgatttga acgacgcggt aagcagcttc gggatcgtcg
      241 ccgttgatgt gaaagatcgg caactgagtc atccgcgcca cgtcagtcga atagatggtg
      301 gaccggccgg cttccggacc ggtcgtgaat ccgatctgat tgttgatgat gatgtgaatc
      361 gtgccgcccg tccgatagcc tttgaggccg gcgaggttca gagtctccat gacgatgccc
      421 tgacccgcaa atgcggcatc gccgtggagc agcaccggca atgcgagatc catcacttgc
      481 tcgcgcgtgg ccccgcgctg gcccatgatc tcgtcctgct ttgcccgcac cattccttcg
      541 acaaccggat cgaccgcttc gagatgactt ggatttggcg acagagtgag ttgaactttg
      601 cgcccatcgg caagcgtgcg ttctccggtc gcgccctggt ggtatttcac gtcgccttca
      661 tcagccggaa actccggatg caccgaacct tcaaacgccg caaagattcg ctcgcagagt
      721 ttgccgatca cgttcgccag cacgttcaaa cggccgcgat gggccatccc gagaacgatc
      781 tcctgcacgc cacgttcgcc ggcgccatcg atcagtttgt cgagcattgg aatgatcgtc
      841 tcgcagcctt cgagtgaaaa tcgtttctgg ccgaggtact tagtgtgcag aaaacgctcg
      901 aactgctccg ccgagattag tttccaaagc aagcgcttct tcaattcgac tggcaacggt
      961 tcgggctgca ctaactcgcg cctaatctgt tcgcgaatcc acgtcttctc ttctttgctc
     1021 tggatgtgcc gatattcgat gccgactttt ccgcagtaag cgcgttgcaa tacgtcgaga
     1081 atctcgcgca acgtcgacgc ttgcttgtta gccaaaccat ccgtcgtaaa aacccgatcg
     1141 agatcccaga tcgtcaagcc gtacgtttcg atgtcgactt cgggatgata gagccggggc
     1201 agcgcatgca acggatcagt gtcggcgatc aaatgacccc gaacgcgata agcgttgatc
     1261 aactcgatga cgcgtgcctc tttgatactt tgctcgagga cctgatcgcc ttgcgtgaag
     1321 aacggattgc gatcgaggtt ccaacgaaac ggtacgtgcg gtatcccaag gtcggcgaag
     1381 atcgtgtcgt agaactcgtg tttgccgatg atgaattcgt gtatccgcgc cagaaatgct
     1441 cccgactccg cgccctgaat gatgcggtga tcgtacgtgc tgctgatcga gatggccttg
     1501 ctgatgccga gttgggacaa agtttccggc gccatcgcct ggtactccgc cgggtattcg
     1561 attgcgcccg tggcgacaat cagcgactgg ccctccatta atcgtggcac tgatgcgacg
     1621 gtgcctatcg tgccgggatt ggttagcgaa acggtcgtgt tttgaaagtc ggtgacctga
     1681 agttttcctt cgcgagccct ttggaccacg tcgtcgtaag cggacagaaa ctcggagaac
     1741 gtgaggctat ttgcgttttt gatgttcggc acaagcagtg tgcgcgtgcc gtctttcttc
     1801 gtcagatcca ccgcgacgcc gagattcact tccggacgtt tcaacctggc gactccgccc
     1861 tcgccttcaa aaaatccgtc gtttagttgg ggaaacacat cgagcgcacg caggattgcc
     1921 cacgcggtca agtgtgtgta tgacgctttg ccgcggtcgg cttccgcgag atgtttgttg
     1981 atgaggcgac ggttttcgtc gagcagctta accggaattc ttcgttcgga agtcgcggtg
     2041 ggaacggaga gactacgctc catgttttcc acgatcttca gcgccgggcc gcgaatggga
     2101 acgacttcaa cgttctctgg cggacgcgcc tcaaccggtt tgcgttcagg agcgggcgcc
     2161 gctgccgcgc tcgtttcttt aacggctgcg cgaacggttc tatcgctttc gacaacggcc
     2221 gctttcgcac cgttattgaa ggttcgggcg gcgccttcgc catcaccaag tatttcgttg
     2281 aagtaggtgc gccaggagtc gtcgacgagg tcaggattgg tacgaaaacg ttcgagcagg
     2341 ccctggacgt actcggcgtt tgcgccaaat tcctcggcga tcatctctga caagtcagtt
     2401 ctgctttgct ggctcaatct gcttacctgt cttcaaaatc tatgtagttt gtttgaggaa
     2461 tagactatca tctccgccat ccatttgcac ttaagccact ttctccgtct caaactcaag
     2521 tacacgtgaa ggctatgaaa aaaaagacca accaacgcaa cgctaagaag tccggcgcca
     2581 atgtcgaccg gcgaaacttt gtcaaagcgc ttccggcgtt cggtatcgcc ggagtagctg
     2641 cttcgaggct gccgtttgac gtgttggccc aaacaccggc tgcgtcgcca acaccgacac
     2701 cgtcgccatc accgtctcca actcccacgc cgcgcatcac caaagagatg atgcgccagg
     2761 ccgagaagct catcggcatc gagcttaccg acgcgcagga agcgatggcg gtcggcggcg
     2821 ttaaccgaaa cctcgactca tacgaggcga ttcgcaaggt cgacgtgccc ctcgacactg
     2881 aaccggcgat tgcttttcac ccttcgcgag cgaagaagga actgtacgtt cagcgatcca
     2941 agtttcgttt tgcgaaggtt gagccgccgc ccttcaagac cgtcgaggac ctcgctttcg
     3001 cgactgtgcc gcagcttgcg gaattgattc gcacgcgccg aatcacttcg acggagttga
     3061 cgaagatgta tctggggcgg ttgaagcgct atggaccaaa gttactttgc gtcgtcacgc
     3121 tcaccgagga attggcacta aaacaggccg cggccgcaga tgccgaactg aagcgcggca
     3181 agtatcgcgg gccgctgcac ggaattccct ggggcgccaa agatctcttc gcgacgaaag
     3241 gcatcaagac cacgtggggc gccgaacctt atcgcgatca gatgattgat tacgacgcaa
     3301 cggtcattga gagactgaat gaagcaggcg cggtgctggt ggcgaaactg tcgatgggcg
     3361 cgttggccca gggcgcgaga tggttcgcag gcgtgacgcg taatccgtgg caacctgacg
     3421 aagcgcaaac aggatcgagc ggctcgtctg ccggcgccgc t
//
LOCUS       DASTEY010000164         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_794829, whole genome shotgun sequence.
ACCESSION   DASTEY010000164 DASTEY010000000
VERSION     DASTEY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794829"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..450)
                     /locus_tag="VFO72_02910"
     CDS             complement(76..450)
                     /locus_tag="VFO72_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478266.1"
                     /translation="MTTDKKIPFRFVLEELEPIRPRVKRAFGFTYVYLDEKLLCGLRE
                     SVKLPSCNGLWLFTTTEHVDSLWREFPELPRRYLWRSGKKAWVILASRLEFFEEYAFK
                     ACELMLNGDSRVGRVDVRGPGR"
     gene            500..907
                     /locus_tag="VFO72_02915"
     CDS             500..907
                     /locus_tag="VFO72_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9478267.1"
                     /translation="MAPTLYRIILQVDNLDRAEEFYGKLLGDRGRRIHGASRHYIDCG
                     PVILALVDVNGENIPGLEPKPLPDYIYFAVNEIKSFHARARKLNCLSTEDVHGDSAGD
                     IVTRPWRERSFYAHDPWGNGLCFVDETTLFTGR"
     gene            1027..2181
                     /locus_tag="VFO72_02920"
     CDS             1027..2181
                     /locus_tag="VFO72_02920"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478268.1"
                     /translation="MSRNPTYQAASIAAPEIEAHFKRHLANARKQGQDGLAPQPDAPS
                     IARIIDATFWASFRPEEGRFPKISLAYLPPEEAGQPLMFARPLPLTAAVLTKLAPAVE
                     RPEIHLGVWTFDNELRVWGATRTIPSFCFVLEDVEPGLLVVKHRRLDGFGKYANVAVF
                     KGERVKIIDEKGTSLPDCPSLLRALLAFTAAATNGPQHSLNVLVQLAASMRAQGHGGS
                     LLVVPAGSDRWRESIVQPIVYSVAPSFSALSELVRNEVDVDHEDRRQWESALRRAVDT
                     IGGLTAVDGATVINDQYELLAFGAKIRPPRESPLVDKWVITEPIVGNVGIVVQPTEHG
                     GTRHLSAAQFVYDQRDALALVASQDGRFTVFAWSPCEEMVHAHRVESLLM"
BASE COUNT          495 a          690 c          639 g          485 t
ORIGIN      
        1 acgcgctcga agctcaaacc tgtttccggt tcacgattgt ccattcagtc tcctcaacga
       61 actcagaatt tttgttcatc ttcccggtcc tcgtacgtca actctgccga ccctgctatc
      121 cccgttcagc attagttcac aagctttgaa ggcgtattcc tcgaaaaact ccaggcgtga
      181 ggccagaatc acccacgctt ttttgccgga gcgccagagg taacggcgcg gcagttctgg
      241 aaattcgcgc caaagactat caacgtgctc agttgtagta aacaaccaaa gcccattgca
      301 cgacggtaac ttcacgctct cgcgcaaccc acagagcagt ttttcgtcga gataaacgta
      361 agtgaaacca aacgcgcgtt tcacccgtgg ccggatcggc tcgagttctt cgagcacgaa
      421 gcgaaacggg attttcttat cggtagtcat ctgcgttaat ctgtggcgaa agtttcaacg
      481 gtggaaagga cccgcagcaa tggcccccac actctatcga atcattctgc aagtcgacaa
      541 tctcgaccgc gctgaagagt tctacggcaa gctgcttggc gatcggggac gaagaatcca
      601 cggcgcctct cgtcactaca tcgattgcgg cccggtgatc ctggcgctgg tcgacgtcaa
      661 tggtgaaaac attcccggcc tcgagcccaa accactgccc gactacattt acttcgcggt
      721 aaacgagatc aaatcatttc acgcacgcgc gcggaagttg aactgtttgt cgacagaaga
      781 cgtacacggc gacagcgccg gcgacatcgt cactcgcccg tggcgcgagc gatcgtttta
      841 cgcgcacgac ccgtggggca acggcctctg ctttgtcgat gaaactacac tgttcaccgg
      901 acggtaacgg gcgtctcttt cgggcaccgc aggcgtctcg cccgcatcgc ttcgtaagta
      961 gctttcgccg ctgctatgca ggcgagacgc ctgcggtccc aggaacacgg cgtccaccaa
     1021 tgtaagatga gtaggaaccc aacgtatcaa gcggcaagca tagctgctcc cgaaatcgaa
     1081 gcgcatttca agcgacacct ggcaaacgca cgcaaacagg gccaggacgg tctcgcgcca
     1141 caacccgacg cgccgtcgat cgcgaggatc atcgatgcca cgttctgggc cagttttcgt
     1201 cccgaggagg gccgttttcc aaagatctcg ctggcgtatc ttccgccgga agaggcggga
     1261 cagccgctga tgtttgcacg tccgctgccc ttgacggccg ccgttctgac caaactcgcg
     1321 cccgcggttg aacgtcccga gatccatctc ggagtttgga cgtttgataa cgaactacgg
     1381 gtctggggcg cgacccgcac gattcccagt ttctgtttcg tgctcgaaga cgttgaaccg
     1441 gggctgctcg tcgttaagca tcgccgtctt gacggtttcg gcaaatatgc aaacgttgcg
     1501 gtgttcaaag gcgagcgggt gaagatcatc gacgagaaag gcacgagtct ccctgactgt
     1561 ccttcgctct tgcgagccct gctcgctttc acggccgcag ctacgaatgg gccacagcac
     1621 tcgttgaacg tgctggtcca actcgccgcg tcgatgcgcg cccaaggcca tggcggcagt
     1681 ttactcgtgg tgccggcggg aagcgatcgt tggcgcgaat ctattgtgca gccgatcgtc
     1741 tattcggttg cgccttcgtt ctcggcactc tcggaactgg tgcgtaacga agtagacgtc
     1801 gatcatgaag accggcggca atgggagagc gcgttgcgac gcgccgtcga taccatcggc
     1861 ggactcactg cggtcgacgg cgccaccgta atcaacgatc agtacgaatt gctcgccttt
     1921 ggagcgaaga tcagacctcc gagagaaagc ccgctggtcg acaagtgggt catcacagag
     1981 ccgatcgtcg gcaatgtcgg catcgtggtc cagccgacgg aacatggcgg cacgagacac
     2041 ttgtcagcgg cgcagttcgt ttatgaccaa cgcgacgcgc tcgccctcgt tgcttcacaa
     2101 gatggacgct tcacggtctt cgcctggtcc ccatgcgaag agatggttca cgcacatcgc
     2161 gttgaatcgt tgttgatgtg agccacagat aacacggact atgcgctacc acctctaccg
     2221 catcagcaat tggtcacggt gtgaacatgc gccggaactt gatgccggag tagttcaatt
     2281 gcattgcgcg gccgtcgacc attgattcg
//
LOCUS       DASTEY010000165         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1074957, whole genome shotgun sequence.
ACCESSION   DASTEY010000165 DASTEY010000000
VERSION     DASTEY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074957"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFO72_02925"
     CDS             complement(<1..921)
                     /locus_tag="VFO72_02925"
                     /inference="COORDINATES: protein motif:HMM:NF016021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478269.1"
                     /translation="MGTLNLDRYHAAMGDASFKDVTRLHDRPLSDAKATFYVTQRKLP
                     FAPCLGHERLVRLLVNSQIDRPRLRFLEQDRGGLKLFAKAIEDIQFLGKIRTVRPGTI
                     VFAQEPFADISGAFGMTQAQEIKFEHAFDLPMTTASTAMQFRMAAGEDRWLSDFSLRR
                     NGDIERAVDIAVYAFIGGFNDTSNLEAAFRLDIPAVGTEAHYWQQAYVEYMYRPEIEP
                     RTGKPKHFEQVAFERWLDANPNGTTLLLDTIDVYMGAIHAAMAATSTEARRHAFKGFR
                     VDSGNLAQLGQWCLRFFAANGLTGLRANLTG"
     gene            complement(954..1523)
                     /locus_tag="VFO72_02930"
     CDS             complement(954..1523)
                     /locus_tag="VFO72_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HET9478270.1"
                     /translation="MANTKQALIVVDVQNDFCPGGTLAVPHGDEVVAPLNKLIDEFLE
                     RGDPVYKSRDWHPPTTKHFQAYGGTWPVHCVQNTRGAEFHPALRDDPRIKVISKGLGD
                     TDCYSAFDETNLGAELENAGVKELVVGGLATDYCVKNTVIDALKRGFKVKAVEEAMRP
                     VELQPGDGEKAIEEMRAAGAEIVSTDSTD"
     gene            1582..>2076
                     /locus_tag="VFO72_02935"
     CDS             1582..>2076
                     /locus_tag="VFO72_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478271.1"
                     /translation="MNSAEFFPDSLAARIDKALQDAQTANIAQRIIAKDPTVWTTDES
                     VAKMIRNSLGWLTVAGEMRGVVGELREFAEEARRKFRHVMVCGMGGSSLCPEVLARTF
                     GQQPGFPELLVLDSTDPDVIASFVERINVEQCLFVIASKSGTTTEPNVFYKFWQERVK
                     SGENF"
BASE COUNT          437 a          549 c          615 g          475 t
ORIGIN      
        1 gccggtgagg tttgcgcgca gtcccgtcaa gccgttcgca gcgaagaatc gcaggcacca
       61 ttggcccaac tgcgcgagat tcccggagtc gacgcgaaag cctttgaatg cgtgacgccg
      121 cgcttcggtg gaggtcgcgg ccatcgcggc atgaatcgcg cccatgtaaa cgtcgatcgt
      181 gtcgagcaac aaagtggtgc cgtttggatt agcgtcgagc cagcgttcga aagcgacctg
      241 ttcgaaatgt ttcggcttgc ccgtgcgcgg ctcgatctcg ggccggtaca tgtactcgac
      301 gtaagcctgt tgccagtaat gtgcttcggt gcccacggcg ggaatgtcga gacgaaacgc
      361 ggcctcgaga tttgaagtgt cgttgaagcc gccgatgaaa gcatagacag cgatgtcgac
      421 agcgcgttcg atgtcgccgt tgcgacgcag cgagaagtct gacaaccagc gatcttcacc
      481 ggcggccatg cgaaactgca tcgcagtgct agcggtcgtc atcggcagat cgaacgcatg
      541 ctcgaatttg atctcctgtg cctgtgtcat gccgaacgca ccgctgatat ccgcaaacgg
      601 ctcctgcgca aagacgatag tgcccggccg cacggtccgt atcttgccga gaaactgaat
      661 gtcttcgatc gctttggcga agagtttcag accgccgcgg tcctgttcga ggaaacgaag
      721 tcgcgggcga tcgatctggc tgttgaccag gagacgaacg agacgctcgt gcccgagaca
      781 cggcgcaaag ggaagtttcc gttgggtaac gtagaaggta gctttggcgt cgctgagtgg
      841 tcgatcgtga aggcgggtta cgtctttgaa cgacgcatcg cccatggccg catgataacg
      901 atcgaggttt aaggtgccca tgggaaaacc atccacagat tacacggaga ggtctaatct
      961 gtggaatctg tggatactat ttcggcgccg gcagcacgca tctcttcgat agctttctca
     1021 ccatcgcccg gctgcaactc gaccgggcgc atcgcttctt cgaccgcctt gaccttgaac
     1081 ccccgcttaa gcgcgtcaat taccgtgttc ttgacgcaat agtccgtggc caatcccccg
     1141 acaaccaatt ctttgacgcc tgcgttttcc agttctgctc ccaggttggt ttcatcaaac
     1201 gcggagtagc aatcggtatc gcccagccct ttcgaaatca ctttgatacg cggatcgtcg
     1261 cgcagcgcag gatgaaactc cgcgcctctc gtgttctgaa cgcagtgcac gggccatgtg
     1321 ccgccgtaag cctgaaagtg tttggtagtt ggcgggtgcc agtcgcggct cttgtaaacc
     1381 ggatctccgc gttcgagaaa ctcgtcgatc aatttattca aaggcgcgac aacctcatct
     1441 ccatgcggca ctgcgagtgt gccgcccggg caaaaatcgt tttgtacgtc aacaacgatg
     1501 agcgcctgct tggtattggc catgatgtgt cgatgttaca ctgattgcgc acactctaaa
     1561 agccgaggaa ttgaactgaa gatgaactcc gctgaattct ttcctgattc gcttgcagca
     1621 cgcattgaca aggcgcttca agacgcacaa accgcaaata tcgcgcaacg aatcattgcc
     1681 aaagacccta ctgtttggac cactgacgag tcggtcgcga agatgatccg aaactcgctt
     1741 ggttggttga cagttgcggg tgagatgcgc ggcgtagttg gtgaattgcg tgaatttgcg
     1801 gaagaggcgc ggcggaagtt tcgtcacgtg atggtctgcg gcatgggtgg ttcgtcgttg
     1861 tgtccggagg tgctggcgcg aacgttcgga cagcaaccgg ggtttcccga gttgttggtt
     1921 ctcgattcga ccgacccgga cgtgattgct tcgtttgttg agaggatcaa tgttgagcag
     1981 tgtctgtttg tgattgcttc gaagtctgga actacgacgg agccgaacgt cttttataag
     2041 ttttggcagg agagggtgaa atcgggcgag aacttt
//
LOCUS       DASTEY010000166         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1206622, whole genome shotgun sequence.
ACCESSION   DASTEY010000166 DASTEY010000000
VERSION     DASTEY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206622"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..2026)
                     /locus_tag="VFO72_02940"
     CDS             complement(167..2026)
                     /locus_tag="VFO72_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET9478272.1"
                     /translation="MNNLATDIDVARPRLPAQRSFAPDEPATLIDVFTRAARVHNRPD
                     ALNYKSGNRWFAVSSDEMLSRAKHIAAGLHAIGIRHGDRVALLSDSRVEWTLTDAGCL
                     FASAVDVPIYPTLTPSQVRYILRDSGASALFLANREKFFELKEILAECPQVKQVIFFN
                     AEGITAAEGLTLVQLEEKGRELEGSDPGLIDRMAAETTSDDLATIIYTSGTTGEPKGV
                     MLSHGNLVSNLIDSTGHLSIGEGDTVLSVLPLSHVYERQAMYMYLHHGMAVYFAESLQ
                     TIGPNLREVRPTIVVGVPRMFEKIYARIRERAAEAGKLASALLAWSVSVAREYAQHVS
                     KRRPVPAMLRLKHWIAMKLVFSKWHEAFGGRIRLLLSGGAALSEDLACIYIGAGIPII
                     QGYGLTETSPVVTASSLEDYRVGTVGRAIPNVDIRIAEDGEIEVRGPNVMRGYYNKPE
                     ETRAAFTEDGFFKTGDIGTIDEDGFLRITDRKKELFKTSGGKYIAPAPIEQAIKGSRF
                     VNQVVLVGSERKFPAALIVPVWEQLESYCKLKGIEVKSRSELCHHPRIVDLLQRQIDA
                     LTPNLARYEKVKKVALLENEFTIEGGELTPTLKVKRRVIDEKYRDVIEKLYEE"
     gene            complement(2023..2640)
                     /locus_tag="VFO72_02945"
     CDS             complement(2023..2640)
                     /locus_tag="VFO72_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9478273.1"
                     /translation="MRSARTLNNNARLPGGDKREAILRAATRVFAHNGYFNSKVADIA
                     REAGVADGTVYLYFKSKEEILHSIFDRSVDEALAAARKQIQMLTDPREKLRRIALLHL
                     EKLGADRDLAVVFQVELRGSTKFMEEFSAAGFAEYLALIRATFEEGQQAGVFRSDLNA
                     NIVAKILFGALDEMATNWILSKRRYKLAPMADQVLDILLNGVKAS"
     gene            complement(2684..>3389)
                     /locus_tag="VFO72_02950"
     CDS             complement(2684..>3389)
                     /locus_tag="VFO72_02950"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9478274.1"
                     /translation="TLIRYVVIIVGSLVAVKQAFAIDLTSIAVIFTALSVGIGFGLQY
                     LAADIASGFILLFERPIRVGDRITLGEDEGDVQSIRLRTTVVKTNDQIAIIVPNSKLV
                     SQPVINWSYGDPRARIVCEVGVSYDADIDLVTDTLVEAAQGVENVLAAPPPRVQFMKF
                     GEYSLDFRLLVWTHQPRRHAQIRSDINYRIAKLFRERSIKIPYPTQQFLLDGTSLPKD
                     LQPNLLGDDDEPRMQD"
BASE COUNT          725 a          993 c          932 g          739 t
ORIGIN      
        1 ccggtgaaga cgccgccgaa agaatcctga aggcaatgaa cttgaacgtg ccgccagtga
       61 agtaagcgtt ctctcctggg accgcaggcg tctcgcgtgc tggctgcgag gttatgttac
      121 gaaactagca ggcgagacgc ctgcggtccc aggaaagaga acgtccttat tcctcgtaca
      181 acttttcaat cacgtcgcgg tacttctcgt cgattactcg ccgcttaact ttcaacgtgg
      241 gcgtcaactc gccgccttcg atcgtgaact cgttctccag taacgccact ttcttaacct
      301 tttcataacg cgccaggttt ggagtcaatg catcgatctg ccgttgcaac agatctacga
      361 tccgcggatg atgacacaac tcgctgcggc tcttaacttc aatccctttc aacttgcagt
      421 acgactccaa ctgctcccac accggcacga tcaacgccgc cggaaacttg cgctccgatc
      481 cgaccagcac cacctggttc acaaaacgcg agcctttgat cgcctgttcg atcggcgcag
      541 gcgcgatgta cttgccgccg gaagtcttga acaactcctt ctttcgatcc gtgatccgca
      601 gaaaaccatc ttcatcgatc gtgccgatgt cgccggtctt aaagaacccg tcttcggtga
      661 acgccgcgcg tgtttcctcg ggcttgttgt aatagcctcg catcacgttt ggtccacgta
      721 cttcgatctc accatcctca gcaatgcgga tatcaacatt cggaatggcc ctgccaaccg
      781 tcccaacccg gtaatcctct aacgagctcg cagtgacgac gggcgaagtt tccgtcagtc
      841 catacccctg gataatcgga atgccggcgc cgatgtagat gcaagcgaga tcttctgaga
      901 gtgccgcgcc acccgaaagc agcaggcgaa tccgcccgcc aaaagcctcg tgccatttgg
      961 agaacacgag cttcatggcg atccagtgtt taagtctcaa catcgccgga accggccggc
     1021 gtttgctcac gtgctgcgcg tattcgcgcg ccactgacac ggaccacgcc agcagcgccg
     1081 aagccaactt ccctgcttcg gccgcgcgtt cacgaatacg cgcgtagatc ttttcaaaca
     1141 ttcgcggcac gcccacaact atcgtcggtc ggacttcgcg caggtttggt ccaatcgtct
     1201 gcaacgactc cgcaaagtaa accgccatgc cgtgatgcag gtacatgtac atcgcctgcc
     1261 gctcgtagac gtgcgaaagc ggcagcacgg aaagcaccgt gtcgccttcg ccgatcgaaa
     1321 gatggcccgt ggagtcgatg agattcgaga cgagatttcc gtgggacagc atcacacctt
     1381 tcggttcgcc ggtggttcct gaagtgtaga tgatcgtggc gagatcgtct gacgtcgttt
     1441 ccgccgccat gcgatcgatg agaccgggat cgctcccctc caattcgcgc cccttctctt
     1501 cgagctggac caacgtcaaa ccctcggccg cagtgatccc ctccgcattg aagaagatga
     1561 cctgcttaac ttgcggacac tctgcgagta tctctttcaa ctcgaaaaac ttctcgcggt
     1621 tggcaagaaa caacgcgctc gcgccggagt ctctgagaat gtaacgtacc tgcgacggcg
     1681 tcagagtcgg atagatcgga acgtcgactg cgctcgcgaa gaggcagccg gcgtcggtaa
     1741 gtgtccactc aacccggctg tccgaaagca atgcgacgcg atcgccgtga cgaatcccga
     1801 tcgcgtgcag tcccgcggca atgtgcttgg cgcgcgacaa catttcatcg gaggagacgg
     1861 caaaccagcg attgccgctt ttgtagttca gcgcgtcggg ccggttgtgt acgcgagcgg
     1921 cgcgcgtgaa cacgtcgatc agtgtcgccg gttcgtcagg cgcgaacgac cgttgggccg
     1981 gcaaacgcgg ccgcgccacg tcgatgtcgg tggccaggtt gttcacgagg ctttcacccc
     2041 attgagaaga atgtcgagca cctgatcggc catgggggcc agtttgtaac ggcgtttgga
     2101 gagaatccaa ttcgtagcca tttcgtcgag cgcgccaaag agaatcttgg ctacgatgtt
     2161 agcgtttaga tcgctgcgaa acacgcccgc ttgttgccct tcttcaaaag tggctcgaat
     2221 gagcgccaga tattcggcaa aacccgcggc cgaaaactcc tccataaatt tggttgagcc
     2281 gcgcaattcg acttgaaaca cgactgcgag atcacgatca gcgcccagct tctctagatg
     2341 cagcaaagcg atgcggcgta acttttcgcg cgggtcggtg agcatttgga tctgcttgcg
     2401 cgcggcggcg agtgcttcgt ccacactgcg gtcgaagatc gagtggagaa tctcctcttt
     2461 gcttttgaag tagagataga cggtgccgtc ggccacaccc gcttcgcgcg cgatatcggc
     2521 gactttggaa ttgaagtagc cgttgtgagc gaatacacga gtagcggcgc gcaggatggc
     2581 ctcgcgttta tcgccgcccg gcagacgagc gttgttgttg agtgttctgg cggaacgcat
     2641 acggctcgag acactgaatg atgcctcatt cagtggttcg atttcaatcc tgcatgcgcg
     2701 gttcgtcgtc atcgcccaga agatttggtt ggagatcttt cggcaacgac gtgccgtcca
     2761 gtaggaattg ctgcgtggga tacggtattt tgattgaccg ttcgcggaac agcttcgcaa
     2821 tacgatagtt gatgtcactg cggatctgcg cgtggcgccg cggctggtga gtccagacca
     2881 gcaaacggaa atcaagtgag tattctccaa acttcatgaa ttgcacgcgc ggcggtggag
     2941 cggccagcac gttttcgact ccctgcgccg cctccaccag tgtatccgtc acgagatcga
     3001 tatccgcgtc gtaagacact ccaacttcac agactattcg agcgcgtgga tctccatacg
     3061 accagttgat cacgggctga ctgacaagct ttgaattagg cacgatgatc gcgatctgat
     3121 cgttggtctt taccacggtt gtgcgcagcc tgatgctctg cacgtcgcct tcgtcttcgc
     3181 caagcgttat tcgatcgccg acgcggatgg gtctttcgaa aagcaggatg aagccggaag
     3241 ctatatccgc tgcgagatac tggaggccga agccgatgcc cactgacaag gctgtgaaga
     3301 tcacggcaat cgaggtcagg tcgattgcga acgcctgctt gacggcaacc agtgagccga
     3361 caatgattac gacatacctg atcaatgtc
//
LOCUS       DASTEY010000167         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1569087, whole genome shotgun sequence.
ACCESSION   DASTEY010000167 DASTEY010000000
VERSION     DASTEY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569087"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFO72_02955"
     CDS             complement(<1..632)
                     /locus_tag="VFO72_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9478275.1"
                     /translation="MIRIDDIVEKVAANHPQADLDLLRRAYLFSAKEHKGQKRASGES
                     YLVHPLEVANILADMRLDEVSVATGLLHDVVEDTLVELDTIRRYFGEEITHLVDGLTK
                     IAQISNISREEQQAENVRKMLLAMVDDVRVVLVKLADRLHNMRTLQYLSPEKRKRIAE
                     ETMEIFAPIAHRLGMGKLRGELEDLAFRHLYPEDYRELAEQLEKRRPENEA"
     gene            complement(736..1491)
                     /locus_tag="VFO72_02960"
     CDS             complement(736..1491)
                     /locus_tag="VFO72_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478276.1"
                     /translation="MNEAARAYREGNFVEAEQHSRRAAELDPNNRTAPMFIARTIHAQ
                     YRPGVQTPDNVAKAQQAIEAYQKILEKNPADDEAYKAIAYLYGAIKDDAKLRQWITAR
                     ATSDATDPTKRSEAYVVLASKDWECSFQITELPTNKITTVDQATNKATISYRKPKDQK
                     EFEQAQMCVKQGLAEAENAIKFDPNSESAWSYKTNLLLEASKLAEMDGQADQKAQYQR
                     DYEAALKRTTELSAAAEKRREEEARQQASPPKG"
     gene            complement(1617..2138)
                     /locus_tag="VFO72_02965"
     CDS             complement(1617..2138)
                     /locus_tag="VFO72_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9478277.1"
                     /translation="MAMGSMGGGNSKRSSTTAEPYINVTPLIDVLLVLLIIFMVVTPL
                     RPSRFQADIPTQRDPNEDLSRLKPNPLTLVVSIAPDLSIKLNQDSLGSVNDPSALAQR
                     LALVFKQRKETRAYKVGMETATNVKEDDRIEKTVFVKAPRSLRYGDVVKVIDAIKGAG
                     ANPVGLQVDDLPQ"
     gene            complement(2166..2627)
                     /locus_tag="VFO72_02970"
     CDS             complement(2166..2627)
                     /locus_tag="VFO72_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9478278.1"
                     /translation="MSMQAGGGSTGLQSEINVTPMVDIMLVLLIIFMVVTPFLQQGIT
                     VALPKNMNYPEEDPRIIKESSVVISIPENGEYYLGKQRLQREQLGEQVDRMLEKIKNE
                     QDRIVYIKSGVNVDYGDVVNVINEVRKLGVDKIGLVADKKKQGAAAATVAS"
     gene            complement(2767..3537)
                     /locus_tag="VFO72_02975"
     CDS             complement(2767..3537)
                     /locus_tag="VFO72_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9478279.1"
                     /translation="MLMSVFGTKILGLMVLLQDAAAAAGETTTADHFTLTEMVKNMGA
                     VAISVVVVLLIMSVYSIAIMVERYLTYSAAKKQSREFAPRVAQALKNDRIEEAINISD
                     KHRKSHLAMVVSSGLQEFRAHEQSSDISGDEIEASKRALQRAIAIKTAEFKRGLSGLA
                     TIGSTAPFVGLFGTVFGIINAFRGMKNAESAGIAAVAGGISEALFTTALGLLVAVPAV
                     WLFNYFTGKVDGFVVEMDNSASELVDYFIKNRTKQLKA"
BASE COUNT          758 a         1056 c          966 g          936 t
ORIGIN      
        1 gcttcattct ccggacgccg tttttcaagt tgttcggcga gttcgcggta atcttccgga
       61 taaagatgac gaaaagcgag gtcctcgagt tcgcctctca acttacccat accgagccgg
      121 tgtgcaatcg gcgcgaagat ctccatcgtc tcttcagcaa tgcgtttgcg tttttccgga
      181 ctcaaatact ggagcgtgcg catgttatgc agtcgatcgg caagtttcac gagcacgacg
      241 cgcacgtcgt caaccatcgc cagcaacatc ttgcgcacgt tttccgcctg ttgctcctcg
      301 cgcgaaatgt tgctgatctg cgcaatcttg gtcagaccat cgacaaggtg cgtgatctct
      361 tcaccaaagt agcgccggat cgtgtcgagt tctacgagcg tgtcttcaac cacgtcatga
      421 agcaggcccg tcgcgaccga cacttcgtcg agtctcatgt cggcgaggat gtttgcaact
      481 tcgagcggat ggacgaggta cgactctccc gacgcacgct tctgtccctt gtgttcttta
      541 gcggaaaaaa ggtaagcgcg acggagtaga tcgagatctg cctgcgggtg attagcggcg
      601 accttctcga caatatcgtc aatccgaatc attttcagca gcaaagtata acaaaggcca
      661 cagaccgtca cgaacaacac cgatcagcgt gttggtcgtg aaaatctgtg gccttttggt
      721 gaaactgagg ggagcttaac ccttgggtgg tgaagcctgt tgccgggctt cctcttccct
      781 ccgcttttcg gcggccgcac tcaactcggt agtgcgttta agagcggctt cgtagtccct
      841 ttgatactgg gccttttgat cggcctgccc gtccatctcg gcaagctttg aagcttcaag
      901 caggaggttg gtcttgtaag accaggccga ctcgctgttt ggatcaaact tgatcgcgtt
      961 ctcggcttcg gcgagtccct gcttcacgca catttgcgct tgctcgaatt ccttctgatc
     1021 cttgggcttg cgataggaga tcgtcgcctt gttggtggcc tgatcgaccg tggttatttt
     1081 gttggtcggc agctcggtga tctggaacga acactcccag tctttgctgg cgaggactac
     1141 gtacgcttcg gatcgcttgg ttgggtcagt cgcgtcgctg gtggcgcgag cagtgatcca
     1201 ctgtcgcagt ttcgcgtcat ccttgattgc gccgtagaga taagcgatcg ctttgtaagc
     1261 ctcgtcgtca gcaggatttt tttccagaat cttctgatag gcttcgatcg cttgctgggc
     1321 cttggcaacg ttatccggcg tttgcacgcc cggacggtat tgcgcgtgaa tagtgcgcgc
     1381 gataaacatc ggcgccgtcc tattgttcgg atccagctcc gccgcccggc gcgaatgctg
     1441 ttccgcttcg acgaagttgc cttcgcgata agcgcgtgca gcttcgttca actgattctt
     1501 cgatctgatt ctattaatga ctccgcaccc ggagctggtg gcaacaagga tcgccaaggc
     1561 aatgccaatg gcgatcctgg tctgagcgag tttcatttat gcgtgactcc aaatatttac
     1621 tgaggcagat cgtccacttg caaacctact ggatttgcgc cggcgccttt gatcgcatcg
     1681 attactttca ccacgtcgcc gtaacgcagc gatcgcggcg ctttcacgaa gacggttttc
     1741 tcgattcgat catcttcttt gacgttggtc gccgtttcca ttcccacctt gtacgcgcgt
     1801 gtctcttttc gttgcttgaa aacaagcgcc agtcgttgcg ccaaagctga agggtcgttg
     1861 acggatccca aagaatcctg attcagctta atcgaaaggt caggcgcgat cgagactacc
     1921 agcgtcagcg gatttggttt caatctgctg aggtcttcat ttggatcgcg ctgcgtcgga
     1981 atgtcggcct ggaaccggct cggcctcaac ggcgtgacca ccatgaagat gatcagcaag
     2041 acaagcaaaa cgtcaatcaa gggtgtgacg ttgatataag gctcggctgt cgtactgctc
     2101 cttttggaat tacctccacc catcgaaccc atcgccatct caatatctcc tcgttctaat
     2161 tacgtttagg aagctaccgt ggcagccgcc gcgccctgct tcttcttgtc agccacgagc
     2221 ccgatcttat cgacgccgag ctttcgcact tcgttgatga cattgactac gtcgccataa
     2281 tcaacgttca caccgctctt tatgtaaacg atgcgatcct gctcgttctt gatcttctcg
     2341 agcatcctgt cgacctgctc acccaactgc tctctttgca gtctttgttt gccgagatag
     2401 tactcgccgt tttcaggaat cgagatcacg acggacgact ccttgatgat tcgcggatct
     2461 tcctccgggt aattcatgtt cttgggcagg gccactgtaa taccctgttg caaaaacggc
     2521 gtgaccacca tgaagatgat cagcaacacg agcatgatgt cgaccatcgg ggtgacgttg
     2581 atctccgact gtaggccggt acttccgcct ccagcttgca ttgacatagc aaatctcctt
     2641 tttaatccgg taaacgccgg tgcggcttag ctatcgaacg cccgttcgac ctgggctgtc
     2701 gaacgcctgt tcgtttgtcg ccgcacctca gggttcacca ttccccgcgt ttgtgacgcg
     2761 tataacttaa gccttcaact gcttcgtgcg gttcttgatg aagtagtcga ccagctcaga
     2821 agccgagttg tccatctcga cgacaaagcc atcgacctta ccggtaaagt agttgaacag
     2881 ccacaccgcg ggcaccgcta cgagcaagcc gagcgcggtc gtaaacaacg cttccgaaat
     2941 accgccggcg actgccgcga taccggccga ctcggcgttc ttcatgccgc ggaaagcgtt
     3001 gatgatgccg aacacggttc cgaacagacc cacgaacggc gcggtcgaac caatcgtcgc
     3061 cagaccggac agtccacgct tgaattcggc tgtcttgatc gcgattgcac gttgcaacgc
     3121 gcgttttgac gcctcaatct cgtcacccga gatgtcggac gattgttcgt gcgcacgaaa
     3181 ctcctgcaaa ccgctgctga ccaccatcgc caggtgcgat ttgcggtgtt tgtcgctgat
     3241 gttgatcgct tcctcgatcc gatcgttctt cagcgcctgc gcaacgcgtg gtgcaaactc
     3301 gcgcgactgc ttcttggccg cggaatatgt caggtagcgc tcgaccatga tcgctatcga
     3361 atagaccgac atgatgagta gcacgaccac aacggagata gccacggcgc ccatgttctt
     3421 caccatttca gtcagtgtga aatggtcggc ggtagtcgtc tccccggctg ctgctgccgc
     3481 gtcctgcaac agcaccataa gaccgaggat cttggttcca aaaacactca ttagcattgt
     3541 cacggtgaac ccctccaaaa ggttttatta gttttatagc aagcagagaa gccgggacgg
     3601 cctacggtaa ccgcggcgac acgacaaaag ttctccgcag ttattgagcc acgaagttgt
     3661 actggatcac tccggtaact ttaactggct gccccgaaag cttggttggg gagaat
//
LOCUS       DASTEY010000168         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1107792, whole genome shotgun sequence.
ACCESSION   DASTEY010000168 DASTEY010000000
VERSION     DASTEY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107792"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1975
                     /gene="mutL"
                     /locus_tag="VFO72_02980"
     CDS             167..1975
                     /gene="mutL"
                     /locus_tag="VFO72_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9478280.1"
                     /translation="MAKIRVLADHVANQIAAGEVVERPASVAKELVENSIDAGATHIT
                     IEIEAGGRRLLKVSDNGEGMVRDDAILAFERHATSKIRGAEDLIAIGTLGFRGEALAS
                     IASVAKVELTTSTEGANAATRVAIEGGRMRDVKDAAHPRGTTIAVRDLFFNVPARRKF
                     LRSEATETFHLTNLVTHYALAHPEIAFVFMNNGRDVVRAVAAKDLCERAYQIFGEEFL
                     ENLLAVDGGDPLVARVTGYVSAPRDRRTSRDSQYLFVNRRFVRDRMIGRSLSEGYRSV
                     LPHGVYPAALLFIETPLEEVDVNVHPAKAEVRFRRSAAVSDAVREAVRDALARNEPQI
                     PQTPQIPQTPQTQTASAAVSLTPPQPRIEFAPLPTGEEIARDIEEMVRVSSLKSVVKT
                     PPLDSAAKLVREVSPETLSANIRPLGQLDESFIIATDDEGLLLIDQHVAHERVLFDKY
                     RALESERRTESQQLLVPETFDLTPAQAAVFDQLAPELEPYGFELMRLSGRTVAIRAVP
                     ADLPAGEARNMLSELLDTVDAEKKSSARETLRDEIAASLACHAAIKVNMPLAPEKMRW
                     LIDRLLQTSSPTTCPHGRPIILRLQMRDILKGFHRI"
     gene            complement(2015..2470)
                     /locus_tag="VFO72_02985"
     CDS             complement(2015..2470)
                     /locus_tag="VFO72_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9478281.1"
                     /translation="MKLKAIIGETEEELDLRLDGGRVSARVGARSYDLEAHEIEPGFY
                     LLFVGNRVHECRVDGTIKIIDPKRLRSGQNSSGHHHGVAQIVAPMPGKVVRVQVEAGA
                     TVEKGAGVVVVEAMKMQNEMKSPRAGVVLSVNVQPGDTVNAGDVLAVLA"
     gene            complement(2445..>3475)
                     /locus_tag="VFO72_02990"
     CDS             complement(2445..>3475)
                     /locus_tag="VFO72_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478282.1"
                     /translation="LKAAAGGGGKGMRLVNDAHELRSAFETAQTEATAAFGDSSLYLE
                     KAVERPRHIEIQIFADNHGNVVHLGERECSIQRRHQKVIEECPSPINDVDLRRRMGEA
                     AVKIARAVDYTGAGTVEFLLSDTTREFYFLEMNTRLQVEHPVTELVTGFDLVREQFSV
                     AAGRKLSFSQEDVRWVGHAIECRIYAEDPANNFFPSPGIITHLQEPLGPGIRIDSGVQ
                     RLSEVSIHYDPMIAKLAVWGRTREEAIDRLRRALDEYEVAGITTTLPFFREIVRDEEF
                     IAARLDTGFISRFNERATGPQISTEDTDLAIIAAALHYAKHEQPVRVQNRVSKWKLAG
                     REIKSNHR"
BASE COUNT          732 a         1060 c          934 g          749 t
ORIGIN      
        1 agttacagcg tgccttcgat caactctatc caaacaaaga tttacaggag cgtcacatca
       61 acatcacgtc gctgctgacc cgccacggca cgtatgttat agagtggatc ttcaacgcta
      121 taaacctggg ttctaacgaa caccaaatcg tctatctgta aaatctgtgg ctaaaattcg
      181 agtcctcgca gatcacgtcg ccaatcaaat cgccgccggc gaagttgttg aacgtcctgc
      241 ttcagtcgcc aaggaactgg tcgagaattc aatcgacgcc ggcgccaccc acatcacgat
      301 cgagatcgaa gcgggcggcc gccgcctgtt aaaagtgtcc gacaacggcg aaggcatggt
      361 gcgcgacgat gcaattctcg ctttcgaacg tcacgcaact tcgaagattc gtggagctga
      421 ggatctcatc gccatcggca cgcttggctt tcgcggcgaa gcccttgcct cgatcgcgtc
      481 ggtggcgaaa gttgaactga cgacgagcac tgaaggcgcc aacgctgcga cgcgcgtggc
      541 gatcgaaggc ggccgcatgc gcgacgtgaa ggacgcggca caccctcgag gcacgacgat
      601 cgccgttcga gatctgttct tcaacgtgcc tgcgcggcgg aagtttttgc gatccgaagc
      661 gaccgaaacg tttcacctca ccaacctcgt aacgcactat gcgctggccc atccggagat
      721 cgcgttcgtg tttatgaaca acggccgcga cgtagtccgt gcggtggcgg ccaaagatct
      781 atgcgagcgt gcgtatcaga tctttggcga ggagtttctg gagaatctgt tggcggtgga
      841 tggtggcgat ccgttggtgg cccgcgtgac cggatatgtc tcggcgccgc gcgatcgacg
      901 cacgtcacgc gattcgcagt atctgttcgt caaccggcgg ttcgtccgcg atcgaatgat
      961 cggccgttcg ctttcggagg gttatcgttc ggtcctgccg catggcgttt acccggcggc
     1021 gctgctgttc atcgagacgc cgcttgaaga ggtggacgta aacgtgcatc cggccaaggc
     1081 ggaagtgcgc tttcgtcgct cggctgcggt ttcggatgca gtgcgtgaag ccgtgcgcga
     1141 cgcattggcg cggaatgagc cacagatacc acagacacca cagataccac agacaccaca
     1201 gacacaaact gccagcgcag cagtctcgtt aactccgcca cagcctcgaa tcgagtttgc
     1261 tcctttgccg actggtgagg agattgcgcg agacattgaa gagatggtcc gtgtttcatc
     1321 cttaaaatcc gtggtcaaaa caccgccgct ggattcggcc gcgaagttag tgcgcgaagt
     1381 ttctcctgaa accttgtccg cgaacatccg cccgttgggc caactcgacg aaagcttcat
     1441 catcgccact gacgacgaag gcctgctact gattgatcag cacgttgcac acgaacgcgt
     1501 cctgttcgat aaatatcgcg ccctcgaatc cgaacgccgc actgagtcgc agcaactgct
     1561 ggtccccgaa acattcgatc tgactcccgc gcaagccgca gtcttcgatc aactcgcgcc
     1621 cgaactggaa ccttacggct tcgagctgat gcgtttgtcc ggccgcacgg tggcaatacg
     1681 cgccgtgcct gcggatctgc ctgccggcga agcgcgcaac atgctttccg agttgctgga
     1741 caccgtcgac gcggaaaaga agtcgagcgc tcgcgaaacg ctgcgcgacg agattgccgc
     1801 ttcactcgct tgtcacgccg cgatcaaagt aaacatgccg ctcgctccgg aaaagatgcg
     1861 ctggctgatc gaccggctgt tgcaaacatc atcacccacc acctgcccgc acggtcgccc
     1921 aataatcctg cgtttgcaga tgcgcgacat tctcaaaggc ttccaccgca tctagtgttt
     1981 aaagctcgtt gagacgtttc aacacaagat cggtctacgc caataccgcc aggacatcgc
     2041 cggcgttcac tgtgtcgccg ggctgaacgt taactgaaag cacgacgcct gcacgcggcg
     2101 acttcatctc gttctgcatc ttcatcgctt cgacgacgac cacgccggcg cctttctcta
     2161 ccgttgctcc ggcttcaacc tgaacccgaa caaccttgcc tggcatcggc gcaacgatct
     2221 gggccacgcc gtggtgatga ccgctcgagt tttgaccgct gcgcagtcgc tttgggtcaa
     2281 ttatttttat tgtgccgtcg acccggcact cgtgcactcg gtttcccaca aacagcaggt
     2341 aaaatccggg ttcaatttcg tgagcttcca gatcgtaact tcgcgcgcca acccgtgcgg
     2401 acacgcgtcc accgtcgagt cgcaaatcca actcttcttc agtctcaccg atgattgctt
     2461 ttaatttcac gtcctgcgag tttccatttg ctgacacggt tttgaacgcg aactggttgt
     2521 tcatgtttcg cgtagtgaag cgccgccgcg atgatggcca gatctgtgtc ctccgtggag
     2581 atctgtggcc cagttgcgcg ttcgttgaac cgcgaaatga atccggtatc gagccgtgct
     2641 gcaataaact cttcgtccct cactatctcg cggaagaacg gcagtgtggt tgtgattccc
     2701 gctacctcgt actcatcgag tgcgcgtctc aaccggtcaa tcgcctcctc acgcgtgcgg
     2761 ccccacacgg cgagtttcgc aatcatcgga tcgtagtgaa tcgaaacctc tgacaagcgc
     2821 tgaacgccac tgtcgatccg aattcccggc ccgagtggtt cctgcaaatg cgtgattatt
     2881 ccgggtgatg ggaaaaagtt gttcgccgga tcctccgcgt agatgcgaca ttcaatcgca
     2941 tggcccaccc agcgcacgtc ctcctgactg aacgacaact tacggcccgc agctaccgaa
     3001 aactgttcgc gcaccagatc gaaaccggtc acgagttcgg taaccggatg ttcgacctgt
     3061 aatcgcgtat tcatttcgag aaaatagaac tcgcgagtcg tgtcagaaag caaaaactcg
     3121 accgtgcccg cgccggtgta atcaaccgcc cgcgcgatct ttacggcagc ttcgcccatg
     3181 cgtcgacgca aatcgacgtc gttgattggc gacggacact cttcaatcac tttctggtga
     3241 cgccgttgaa tcgaacactc gcgctcgccg agatggacca cattgccgtg attgtccgcg
     3301 aagatctgaa tctcgatgtg gcgtggacgt tcgaccgctt tctcgagata aagcgacgaa
     3361 tcgccaaatg cggccgtagc ttccgtctgc gcagtttcaa atgccgagcg cagctcgtgc
     3421 gcgtcgttga ctagacgcat gccttttccg ccgccgccgg ccgcagcctt caaca
//
LOCUS       DASTEY010000169         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1173633, whole genome shotgun sequence.
ACCESSION   DASTEY010000169 DASTEY010000000
VERSION     DASTEY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173633"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFO72_02995"
     CDS             <1..282
                     /locus_tag="VFO72_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478283.1"
                     /translation="QITQGLTNQRKQILNAALLEVAMNEARIVNNLAANMLNNPGNLG
                     LRPAGEGTPAQQQQPAQSPTASPAASAPATNGPGASSSPVPVRPANANR"
     gene            561..>2152
                     /locus_tag="VFO72_03000"
     CDS             561..>2152
                     /locus_tag="VFO72_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9478284.1"
                     /translation="MLFRLSEVHKSYGVQDVLRGTSLQVNPGEHVGLVGRNGAGKSTV
                     FRLVRGDETADRGDVVRARGVRLGLLDQHVHFKPGSTVHESALAAFGRLQQIEHEMHE
                     LEHRMAEAGDDLEKVLERYSDLQQEFEHEGGFEYAARAEAILQGLGVDRDMWSLETEK
                     LSGGQQNRLGLACLLLAAPDVLLLDEPTNHLDVNAVEWLEEFLQEYESAYVIISHDRY
                     FLDRCCRRIIELENGRASSYTGNYSDYLVEREERREAQQRAYDNQQQLIAKTEEFIRR
                     NIAGQKTKQAKSRRKMLEKLERIDAVRSDQSSGDFRLQAIERTGNHVLTVTDLTIGYP
                     EKMLADGISFILRRGECLGIIGPNGSGKTTFLRTILNKISPLAGEVRWGTKVQIGYYA
                     QQLDDLDDRNEIVMELRRVAPSTATAGELRSFLAKFLFTGDDVYKHVRDLSGGEKGRL
                     ALAKLIYSRVNVLVLDEPTNHLDIPSREALEEALAAYEGTIVTISHDRYFLDRVATQI
                     LALDGLGGAEHYDGDYTEYHDW"
BASE COUNT          484 a          587 c          653 g          428 t
ORIGIN      
        1 cagatcacgc agggtctcac taatcagcgc aagcagattc tgaacgccgc gttgctcgaa
       61 gtggcgatga atgaagcgcg catcgttaac aacctcgcgg caaacatgct gaacaacccc
      121 ggcaacctgg gtctgcgtcc ggcgggcgaa ggcactccgg cacagcagca acagccggca
      181 caatcgccca cggcatcgcc ggcggcttca gctcccgcaa cgaacggtcc gggcgcgagc
      241 agttcgcctg ttccggtgcg gcctgcgaac gcgaaccgct agatccgtgg gtcgagtcga
      301 tccacctggc cagtcaatcc gctggcagcg agtagatttg caaaccggga cgcggccgga
      361 tagccagtcc gcccttttat tctcacccgg ctttagccgg gtggcagtgt cggaagattc
      421 gaaaaaccgt ttcaacggtt ttcgaacggg cgcggggaac cgttgaaacg gttcgtcaca
      481 agatactaga tcgtaacacc cggctaaagc cgggtgagaa taaaaagggc cttcgtgatc
      541 ccgtctgtgc agtttagtgg atgctgtttc gtctttcaga agtacataaa tcctacggcg
      601 ttcaagacgt gttgcgaggc acgtctttgc aggtcaatcc gggagaacac gtcggacttg
      661 taggtcgaaa tggcgccggc aagagcacgg tctttcgtct cgtcagaggt gacgagacgg
      721 cggatcgtgg cgatgtggtg cgcgctcgtg gcgtccggct cggcctgttg gaccagcacg
      781 tgcacttcaa acccggctcc accgttcacg aatcagcact ggccgcgttt ggacgtctcc
      841 agcagatcga gcacgagatg cacgagctgg agcatcgcat ggccgaggcc ggcgacgatc
      901 tggagaaagt gctcgaacgc tacagcgatc ttcagcagga gtttgagcac gaaggcggct
      961 ttgaatacgc tgcgagagcc gaggcgattc tccagggact cggtgtcgat cgtgacatgt
     1021 ggtcgctgga aaccgagaag ctttcaggcg gccaacaaaa tcgtttgggc cttgcctgtc
     1081 tgctgttggc cgcgcccgac gtgttactgc tggacgagcc caccaaccat ctcgatgtaa
     1141 acgcggtaga gtggttggaa gagtttctgc aagagtacga gtcagcctac gtaatcatca
     1201 gtcacgatcg ctacttcctc gatcgctgtt gccgccgcat tatcgaactc gaaaacggcc
     1261 gcgcgtcgag ctacaccggt aactactccg actacctcgt cgaacgcgaa gagcgtcgcg
     1321 aagcgcaaca gcgtgcttac gataatcagc aacagttgat tgcgaagacc gaggaattta
     1381 tccgccgcaa catcgcggga cagaaaacga agcaggcgaa gtcgcggcgc aagatgctcg
     1441 agaaactcga gcgcatcgat gccgtgcggt cggatcaatc gtcgggagat tttcgtcttc
     1501 aggcgatcga acggaccggg aatcatgtat tgacagtgac ggacctcacg atcggttacc
     1561 cggagaagat gcttgcagac ggtatttcgt ttattctgcg gcgcggagag tgtctcggca
     1621 tcattggacc aaatggatcg ggtaagacaa cgtttctaag aacgatcttg aacaaaatct
     1681 cgccgctcgc gggtgaagtg cgctggggca cgaaagtgca gattggttac tacgcgcaac
     1741 agctcgacga tctcgacgat cgcaacgaga tcgttatgga gttgcgacgt gtcgcgccgt
     1801 cgactgcgac ggccggtgag ctacgttcgt ttttggcgaa gtttctattt accggcgacg
     1861 acgtttacaa acacgtgcga gacctttccg gaggagagaa gggccggctc gcgctcgcta
     1921 agttgatcta ctcgcgcgtg aacgtactgg tgctcgacga gccaacgaac catctcgaca
     1981 tcccctcgcg cgaggcgctg gaagaagcac tcgcggccta cgaagggacg atcgtaacga
     2041 tcagccacga tcgatatttt cttgatcgtg tcgcgacgca gattttggcg ctggacgggc
     2101 ttggcggggc ggagcactac gatggtgatt acactgagta tcacgattgg aa
//
LOCUS       DASTEY010000170         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_976007, whole genome shotgun sequence.
ACCESSION   DASTEY010000170 DASTEY010000000
VERSION     DASTEY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976007"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..1592
                     /locus_tag="VFO72_03005"
     CDS             426..1592
                     /locus_tag="VFO72_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9478285.1"
                     /translation="MDFTTKELLKRYAKLAIGQPFSPVLLNILVTSVCDMRCTHCFFT
                     DELDDRPRKKLQMKTHEIERISQTLGGNLGVLILAGGEPFTRKDLPEIARAFYTNNKL
                     ESIYIMSNGQIQKRIFPDVTRILEECPNLNVTVALGIDGPKAEHEKIRQKPGSWDIAI
                     DTARQLQAIKKEYPRLDVQTCTCFMNSNQDTIFEWYDFLKYDLKPDKVNFNYIRPPSA
                     DPKELEIDHSRYARLAQMIDDDSRHAAIKNNYGGKAGFFKAAIDIYMHGLIAKTQETQ
                     TAQMKCYAGTAGGVVYDEGTVSSCENLAPIGNLRDFDWNFQKLWLSPAMKERRKHAAN
                     GCFCTHESNSYYPSLPFNPGHLIQIKKLEREMKKARREIDGGLGIADNALPIAD"
     gene            1594..2787
                     /locus_tag="VFO72_03010"
     CDS             1594..2787
                     /locus_tag="VFO72_03010"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478286.1"
                     /translation="MSAQPRIPKILIISSDTGGGHRSAAAAIVAGVQKFLDSESYAIR
                     VVRAVEESHHLAEKLVRLYNWILRNRQHWMKYYYWVINRIRPDTRKFFHKRCVGYVRD
                     LFERWCPHIVVSVHPLTQHIFGRVLKELNLADRVPLVSVVTDPCYGFWKGWACDAVTL
                     YLVASEDARRQLIDYGVAPERIKISGMPVHPKFAYPGEEAAQAARRALGLDPDKFTVF
                     VNAGWIGGGNIPQIFRELVRGELDVQAIFLAGKNEDLRAVAESIAVEAPFPVKVIGYS
                     DEIEQLMSAANVMISKLGGLTTFEALACRVPIIGDVITDPMPQEAGTAKLIAQRGAGV
                     MLNRASDVVNVVRRMMEDEVHYSRMRAATAGLALPNATRNIVEEIAALIPQSDNSVLT
                     QVASA"
BASE COUNT          721 a          837 c          853 g          635 t
ORIGIN      
        1 tgcgaaaaac ccgagttgat gaatgagggg gaagttggga agaggatcgc agtctagtcg
       61 ccgcatttgg cgatgtcaat tacagcgaac caagtgtgag ttgcgtcaca gttttgtgtg
      121 gcaaatacac gaatccacga agcaacttag acccttcgaa aatagtgatg cagtttgaac
      181 ccggcgccgg tttcgggacg cgctcttccc tgggaccgca ggcgtctcgc ctgcaaggtg
      241 acgaatcggg cgctctcgtg acatgcaggc gagacgcctg cggtcccagg gaagagcacg
      301 cccaggtaaa ctgaaactgc atcattactc gtccagaaga cctccgttga caacgtaagt
      361 tcaaatagct tactagttaa gttctcgtgc gccgcgttga ggtgcgagaa agaagttgag
      421 ggtaaatgga ttttacgacg aaagaactgt tgaagcggta cgcaaaactc gcgatcggac
      481 aaccgttttc gccggtattg ctaaacatac tggtcacgtc cgtctgcgac atgcgctgca
      541 cgcactgctt tttcactgac gaactcgacg atcgtccgcg caaaaagttg cagatgaaga
      601 cgcacgagat cgagcgcatc tcccaaacac tcggaggcaa tctcggtgtc ttgatcctgg
      661 ccggcggcga accgtttaca cgcaaagatc ttcccgaaat cgcgcgtgct ttctacacca
      721 acaacaagct cgagtcgatc tacatcatgt caaacggcca gattcagaag cgaatctttc
      781 ccgacgtgac gaggatcctc gaagagtgtc caaacctcaa tgtgactgta gccctcggca
      841 tcgatggacc aaaggcggaa cacgaaaaaa tcagacagaa acccggttcg tgggacatcg
      901 ccatcgacac cgcccggcag cttcaggcga tcaagaaaga gtatccgcgg ctcgacgtgc
      961 aaacgtgcac gtgttttatg aactcgaacc aggacacgat cttcgagtgg tacgattttc
     1021 tgaagtacga cctcaagccg gataaagtaa acttcaatta cattcgccca ccttcagcag
     1081 atccgaagga gctcgagatc gatcattcac gttacgcccg gctggcgcaa atgatcgacg
     1141 acgattcgcg tcacgctgcc atcaagaaca actacggcgg caaagccggt ttcttcaaag
     1201 cggcgatcga catctacatg cacggcctga tcgcgaagac gcaggaaacg cagaccgcgc
     1261 agatgaaatg ctacgcaggc acggcaggcg gcgtcgttta cgacgaaggc actgtttcgt
     1321 cgtgcgagaa cctggcgccg atcggcaacc tgcgcgactt cgactggaac ttccaaaagc
     1381 tctggttgtc gccggcaatg aaagaacgtc gcaaacatgc ggcgaacgga tgtttctgca
     1441 cgcacgagtc aaacagttat tacccatcgc tgccgttcaa tcccggccac ttgatccaga
     1501 tcaagaaact cgagcgcgag atgaagaaag cgcgccgcga gatcgatggc gggttgggga
     1561 ttgccgataa cgcattgccg attgccgatt aagatgtccg cgcagcctcg cattccaaag
     1621 atactgataa tttcgtccga cacgggcggc gggcatcgtt ccgctgcggc cgcgatcgtc
     1681 gcgggcgtgc agaagtttct ggacagtgag tcgtatgcga tccgcgtggt gcgcgcggtg
     1741 gaagaatcgc atcaccttgc cgagaagctg gtgcgtcttt acaactggat cctgcgtaat
     1801 cgccagcact ggatgaaata ctactattgg gtgatcaatc gtattcgccc ggatacgcgc
     1861 aagttttttc ataagcgctg tgtcggctac gtccgagatc tctttgaacg ctggtgcccg
     1921 cacatcgtcg tcagcgttca cccgttgacg cagcacattt tcggccgtgt tctaaaagaa
     1981 cttaatctgg cggaccgcgt tccgctcgtg tccgtagtga ccgatccctg ctacggcttt
     2041 tggaaaggtt gggcctgcga cgcggtgacg ctctacctgg ttgcgagtga agatgcgcgg
     2101 cggcaattga tcgactacgg cgtggccccg gaacgaatta agatctcggg catgcctgtg
     2161 catccgaagt ttgcctatcc cggcgaagaa gcggcacagg cggcgcggcg agcactcggg
     2221 ttggaccccg ataaattcac agtctttgta aacgccggct ggatcggcgg cggcaacatc
     2281 ccgcagatct ttcgtgaact tgtgcgcggc gaactcgacg tgcaggcaat ttttcttgcc
     2341 ggtaagaatg aagacttgcg cgccgtcgcg gagtcgatcg ctgtcgaggc gcctttcccg
     2401 gttaaggtca tcggctattc agacgagatc gagcaactga tgagcgcggc aaacgtgatg
     2461 atctcgaagc tcggcggact gacgactttc gaagcgctcg cctgtcgtgt gccgatcatc
     2521 ggggacgtga tcactgatcc gatgccacag gaagctggta ccgcgaagtt gatcgcgcag
     2581 cgtggcgcag gcgtgatgtt gaatcgcgcg agcgatgtgg taaacgttgt gcgccgcatg
     2641 atggaagacg aggtccacta ttcgcgcatg cgcgcggcaa cggctggcct ggctctgccc
     2701 aacgcgactc gtaacatcgt cgaagagatc gccgccctga ttccgcagtc ggacaattcc
     2761 gtcttaactc aagtcgcctc cgcctaaaat tgattgacgg ggataagaaa ccggcgtact
     2821 ttcacacgca cgtagcaacc cagacgttga attaagcgag ctgccgctca gctaacgacg
     2881 ccaaatcttc gagctacgat tgaccaggcc tgccaagcat tcattcagga aggcggcatg
     2941 cccctcctac ctacctcgtg aatcccgcat tgagcctgga cctacgtttc tgtattggtt
     3001 ctttttatgg gctatagcag aaaaccgtat ctcttcacca cgaggc
//
LOCUS       DASTEY010000171         5266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_910179, whole genome shotgun sequence.
ACCESSION   DASTEY010000171 DASTEY010000000
VERSION     DASTEY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910179"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFO72_03015"
     CDS             complement(<1..184)
                     /locus_tag="VFO72_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478287.1"
                     /translation="MAALIALLTLSLTTMAQQRRNFQSQAATSRPAAQADAATVFRSA
                     RDLITDGEWAKAQAKFS"
     gene            complement(235..984)
                     /locus_tag="VFO72_03020"
     CDS             complement(235..984)
                     /locus_tag="VFO72_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478288.1"
                     /translation="MKSEVDQVLLKEYLDGDVAAEEELFARYDRELHVPADLWAGVEQ
                     QIAPPVRASAWWWLRVAAVILFVIGLGSVYLVSDSKDQTIASIKLPEETPVVVQRLAV
                     AVTTNTPIKRMTKRIVRDNPGVLSADPGLLDMDEQDTVKHIEQTENLLRSIRNFPISD
                     TDDEVDVSYDKALSRRLLIENVVLRRDAEMKAKFPTKTLLADLEPFLIDIANLPDHAK
                     PEEVLAIKERVQKTEIVAALLDFQDRGPNNR"
     gene            complement(974..1606)
                     /locus_tag="VFO72_03025"
     CDS             complement(974..1606)
                     /locus_tag="VFO72_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9478289.1"
                     /translation="MKRLTVDELSLEGQPGAFAGTLMNSSADLVTRVCAGDAEAFRLI
                     FERYSRPVISFIFDMVNDRALAEDLTQETFVRAYRAMRTMRRETKLSTWLFGIARNVA
                     RESLRARTRANRHVDLADRSVMDLRDDKAVPVEGVLSKELNELIRRSLASLDEDKRLV
                     FTLKVLHQCSYEEIAEITGFSLAKLKTDLHRARAEMRRRIGSYAGVGYEV"
     gene            complement(1622..3646)
                     /locus_tag="VFO72_03030"
     CDS             complement(1622..3646)
                     /locus_tag="VFO72_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9478290.1"
                     /translation="MTSKTERRPDHIKFQGRILFLTDDTSLIRQQLEATGEAAAQLEA
                     ELAARLTADALPLMGNISTDEITPGWVCFYYDETLGQYVYVGMRESAVQKDEVKNGNF
                     AVVVSGLSKGCGSSRETAPYAEQAAGIQLVIAKSIEKIYGQNSQNIGLLTSTDFGLID
                     RIRRGEDIPLSEFTKELDPISQDIVASGGLFNYNKARLAGKISPPPLETGPRPMTIVE
                     KIVARHAFVTAGKVGVEAVKPGDALFAVADVRFSHEYVTPMAESLFKQALGQDARVSE
                     PESVFAFRDHLTFLGRVMSPKHRQMGLLERAEGLATTQESFTTQQKIKLYGENPEGGS
                     EAICHNAVVEDLALPGQIVVGTDSHTCMAGVLGCFAFGVGSTDMANAWYTKDIRIRVP
                     ETVRYVLKGKKRTDVAAKDVMLHILATEYMKTSKGIGKVLEFAGDDLRNWSMDERATL
                     TNMAVEAGGTTGIIEPDEVTLDYIVRTRDLDPEEVRKGFTFSDPDAEYAAVFEIDLDQ
                     IRPMVATPGDPRNGIPIDELPEEVRIDAAYGGSCTGGKMADMDMYAEVLANALANGQR
                     VAEGVRLYIQFGSQKIKQYARQRGYIEIFQRAGAELIDPSCGACINAGPGASPNAETV
                     TVSAQNRNFPGRSGPGKLYLASPYVVAASAIAGKIVEPADFLKPSPVLSH"
     gene            3734..4381
                     /locus_tag="VFO72_03035"
     CDS             3734..4381
                     /locus_tag="VFO72_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478291.1"
                     /translation="MKVFVSRLALAALIAFAAGSIGMRQFVASGQETDDPVKIATYKR
                     FVDNRLQNPSVAYQAARDYLQRYQKDKDQYTDYLTKWVAAYERDDRKRQLGGLINEKK
                     FDEAYRVGAKILADEPDYLRAQIDLGYAGYLAASNKDESHNAQAVAYARKAIQAIEGG
                     KAPSEWAPFKGKDDTLAYLNYAVGFMTLKSSPDQAIDSLLKAAQYDSDIKKTAST"
     gene            4424..4705
                     /locus_tag="VFO72_03040"
     CDS             4424..4705
                     /locus_tag="VFO72_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478292.1"
                     /translation="MSADYQTKFANKPETPESKAALEKLNVLMDRIIDAYARAIAIAG
                     TDPKTEQSRKEWLGQLTNYYKFRKGGSDAGLTEFIASSVQKPLPPKPQL"
     gene            4706..5131
                     /locus_tag="VFO72_03045"
     CDS             4706..5131
                     /locus_tag="VFO72_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9478293.1"
                     /translation="MVKRLNGNVTDFETLQRLGRAREFIDHCYDHPLSLDQISEKACF
                     SRYHFLRLFRQAFNKTPHQYLIERRIERAKELLGADELRVTDVCFEVGFQSLGSFSTL
                     FHKYVGHAPVTYREKVRQKEQAKRQIPGCFIVMYNLESV"
BASE COUNT         1180 a         1494 c         1414 g         1178 t
ORIGIN      
        1 cgctgaactt cgcctgcgct ttggcccact cgccgtcggt aattagatcg cgcgcactgc
       61 gaaacacagt ggcggcatcg gcctgtgccg cggggcgact ggtcgctgcc tggctttgaa
      121 agttacgtcg ctgctgggcc atcgtcgtga ggctgagtgt gagcaacgcg atcagtgcgg
      181 ccattgacga gattgagata gttttttttc gacattgttt tcgcctctat tgcattaacg
      241 gttgtttgga cctcggtcct gaaaatccag caacgcagca acgatttcag tcttctgcac
      301 tcgctccttg atcgcaagca cctcttcggg tttggcgtga tcgggcaagt ttgcgatatc
      361 gatcaggaaa ggctccaggt cagcgaggag tgttttcgta ggaaactttg ctttcatctc
      421 tgcgtcgcgg cgcagcacta cgttttcgat cagcagcctg cgcgataaag ctttgtcata
      481 actgacatcg acctcgtcat ctgtgtcgga gatcgggaag tttcgaatcg agcgcaggag
      541 gttttcggtt tgttcgatat gtttaaccgt gtcttgttcg tccatatcga gcaggccagg
      601 atccgccgac agcacacccg ggttatccct gacaatccgt tttgtcatcc gtttaatcgg
      661 tgtattggtt gtcaccgcga ctgctagccg ttgaacaact acaggtgttt cttcaggcag
      721 tttaatactc gcgatggttt gatcttttga gtcagaaacc aggtacacgg atccaagtcc
      781 gataacaaac agaatcactg ctgcaactcg tagccaccac caggctgatg ctcttaccgg
      841 tggcgcgatc tgctgttcga ctcccgccca aagatcggcg ggaacgtgaa gctcgcgatc
      901 ataacgcgca aacaactcct cttctgcggc aacatcaccg tcgagatatt ccttcaacaa
      961 cacctgatca acttcagact tcataaccaa ctcccgcata cgaaccaatc cgccgccgca
     1021 tctccgcccg cgcccgatgc aaatccgtct tcaacttcgc caacgagaaa ccggttatct
     1081 cggcaatctc ttcatagctg cactggtgca acaccttcag cgtgaacacc agccgcttgt
     1141 cttcgtcaag agacgccagc gaacgccgga tcagttcgtt caactctttg ctgagcacac
     1201 cctcgaccgg cacggctttg tcgtctctta aatccatgac cgatcggtcc gccagatcca
     1261 cgtgcctgtt ggcgcgtgtg cgcgcccgaa gcgactcacg cgcgacgttt cgcgcaatgc
     1321 cgaaaagcca cgtcgagagt ttcgtctcgc gccgcattgt gcgcatcgcg cggtaagcgc
     1381 ggacgaaagt ctcctgcgtc aagtcctccg ccagcgcccg atcgttgacc atgtcgaaaa
     1441 tgaagctgat aactgggcgg gaatagcgtt caaaaatcag acgaaaagcc tcagcgtcgc
     1501 ccgcgcacac gcgagtaacc aggtctgctg aactattcat cagagttccc gcgaaggcgc
     1561 caggctgacc ttcaagactt agttcgtcga ctgtcagccg cttcatcatg ttcccgctag
     1621 gttagtgaga aagaacgggc gaaggtttca ggaaatcggc cggttcaaca atctttccgg
     1681 caatcgcgga ggcggcgacg acatagggcg acgccaggta gagtttgccc ggaccgctgc
     1741 ggccggggaa gttgcggttt tgggcgctca cggtgacggt ttccgcgtta ggtgaagcgc
     1801 ccggaccggc gttgatgcac gcgccgcacg acgggtcgat caactctgcg cccgcccttt
     1861 ggaagatctc gatataacca cgctgccgcg cgtactgctt gatcttctgc gaaccaaact
     1921 gaatgtaaag acgcacgcct tcggcaacgc gctgtccatt ggccagggcg ttcgccagca
     1981 cttcagcgta catgtccatg tcggccatct tgccgccggt gcacgaaccg ccatacgcgg
     2041 cgtcaatacg gacctcttcc ggtagttcgt cgatcgggat cccgttgcgc ggatctccgg
     2101 gtgtggcaac catgggtcga atctgatcga gatcaatctc gaacacggct gcgtactccg
     2161 cgtccgggtc gctgaaagtg aaccctttgc gcacttcttc aggatcgaga tcgcgtgtgc
     2221 gtacgatgta atcgagtgtc acttcatccg gctcgatgat tccggtcgtg ccgcctgctt
     2281 cgacagccat atttgtcagc gtcgcgcgtt cgtccatgga ccaattgcgc agatcatcgc
     2341 cggcaaactc cagcaccttg ccgatcccct tactcgtctt catgtactcg gtcgcaagga
     2401 tgtggagcat cacgtctttc gccgcgacgt cggtgcgctt ctttcctttg agaacgtagc
     2461 gcacagtttc cggcacacga atgcggatgt ctttcgtgta ccacgcattc gccatgtcgg
     2521 tagatccgac gccgaaagca aagcaaccga gcacgccggc catacacgtg tgcgaatcgg
     2581 tgccgaccac aatctgtccc ggcagggcga gatcttcaac gacggcgtta tggcagatcg
     2641 cttccgatcc accttcggga ttttcaccgt aaagtttgat cttctgttgc gtggtaaaac
     2701 tctcctgcgt cgtggcgagc ccttcggccc tctcaagcag gcccatttgc cggtgcttcg
     2761 gtgacatcac gcggccgaga aacgtcaggt gatcgcgaaa cgcaaacacc gactcaggct
     2821 cggagacacg cgcgtcctgg cccaacgcct gtttgaacag cgactccgcc atcggtgtga
     2881 cgtactcgtg cgagaaacgc acgtcggcta cagcaaacaa agcgtcgccg ggtttgactg
     2941 cttcgacgcc gacctttccg gcggtgacaa aggcatgacg cgcgacgatc ttctctacga
     3001 tcgtcatggg acgtgggcca gtttcgagtg gtggcggcga aatttttccc gcaagacgcg
     3061 ccttgttgta gttgaagagc ccgccggagg cgactatgtc ctgcgaaata ggatcgagtt
     3121 cttttgtaaa ctcagaaagc ggaatgtcct caccgcgtcg aatgcgatcg atcagaccga
     3181 agtcggttga ggttaacagg ccgatatttt gcgagttttg gccgtaaatc ttttcaatac
     3241 tcttcgcgat caccagttga ataccggcgg cctgttcggc gtagggtgcg gtttcgcggg
     3301 atgagccgca gccttttgaa agtcctgaaa cgacgacggc gaagttgccg ttttttactt
     3361 catccttttg cacggcgctt tcgcgcatgc cgacgtagac gtactgaccg agtgtttcgt
     3421 cgtagtagaa gcagacccag ccgggagtga tctcgtcagt ggagatgttg cccataagtg
     3481 gcaaggcgtc tgccgtgaga cgcgccgcga gttccgcttc cagttgcgcg gctgcttcgc
     3541 ccgttgcttc cagttgttgg cggattagac tggtgtcgtc agtaagaaat agaatacggc
     3601 cttgaaattt gatatggtca ggacggcgct cagttttgct tgtcatgtga gccaattata
     3661 accggagctc cataaaaccc ttaacggtct tcgaagcgtc taagccagga ctagttgcgg
     3721 aggaaagtcg aagatgaagg tattcgttag cagattggca ctcgcggcgc tgatagcgtt
     3781 cgcggccgga tctattggaa tgcgccaatt cgtggcgtca ggacaggaga ccgatgatcc
     3841 ggtcaagatc gcgacctata agcggtttgt cgataaccga ttacagaatc caagcgttgc
     3901 ttatcaagcc gcgagagatt acctccaaag ataccagaag gacaaagacc agtacaccga
     3961 ctatctgacc aaatgggtgg cggcctacga gcgcgatgat cgtaaacgcc aactcggcgg
     4021 acttatcaac gagaaaaaat ttgacgaagc ctatcgcgtc ggagcgaaga tactggccga
     4081 tgaacctgac tacctccgcg cgcagatcga tctcggttat gcaggctacc tcgctgcgtc
     4141 caacaaggac gagtcgcaca acgcgcaggc cgttgcttat gctcgcaaag cgattcaggc
     4201 tatcgaaggc ggcaaggcgc cgtccgagtg ggcccccttc aaaggtaagg acgacacgct
     4261 ggcctatctc aattacgcag tcggtttcat gacgctgaag tccagcccgg atcaggctat
     4321 tgattccttg ctcaaggccg cgcagtatga cagcgatatc aagaagacgg cctcaaccta
     4381 gtactttctc gctgtcgctt atgaatccgg tccgtacaag acgatgtcgg ctgattatca
     4441 aacgaagttc gccaataaac cggaaacacc ggaaagcaaa gctgcgttgg agaagctgaa
     4501 cgtcctgatg gatcgcatca tcgatgccta cgcgcgagcg atcgccatcg caggcactga
     4561 tccaaaaact gagcagagcc gcaaggagtg gctggggcag ttgacgaact actacaagtt
     4621 tcgtaaaggt ggcagcgatg cgggtttgac ggagttcatt gccagttcag tacaaaaacc
     4681 gctgccgccc aaaccgcaac tgtaaatggt gaagagactc aatggaaacg taaccgactt
     4741 cgagacgctt cagcgtctcg gcagggctcg cgagtttatc gaccattgct acgatcatcc
     4801 acttagtctc gatcagattt ccgagaaagc ctgtttctct cgctaccact tcctgcgact
     4861 gtttcgccag gccttcaaca agacgccgca ccagtacctg atcgaacgtc gcatcgagag
     4921 ggcgaaagag ctactcggcg ccgatgaact gcgcgtcacg gacgtctgtt ttgaagtcgg
     4981 gtttcagagt cttggatcct tcagcacgct cttccacaag tacgtcggac acgcacccgt
     5041 aacctaccgc gaaaaggtca gacaaaaaga acaggccaaa cgtcaaatcc cgggctgttt
     5101 catcgtgatg tacaacctcg aatccgttta gaggacctgg cacaaaatca cgcccaaaaa
     5161 gcaatttcaa agaagcgttc cctcaccgcc gcacgtatcc ttcacgaaac tttcatcgag
     5221 gagaacgata atgatccgca aaatgtctca cgccacgatt tttgta
//
LOCUS       DASTEY010000172         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1256379, whole genome shotgun sequence.
ACCESSION   DASTEY010000172 DASTEY010000000
VERSION     DASTEY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256379"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFO72_03050"
     CDS             complement(<1..818)
                     /locus_tag="VFO72_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9478294.1"
                     /translation="MQGLVQSTASSKRVAAAVPAVRGRAAVISAPTQIDLREIDFREP
                     AEGEVRVRIEGCGVCASNLPVWQGKPWFEYPLEPGAPGHEAWGYIDAIGLGVVDPAPG
                     DRVAMLSSHGFATYDFAPASQVVRLPASLDEQPCPAEALGCAANIFRRSTILPEETVA
                     IVGIGFLGTLLTQLAVNARAKVFAISRRRSSLTMAEQFGAAATIESSQALEAVKRLTN
                     DLGCDCVIETAGTQESLDLATELTSERGRLVIAGYHQDGPRQVNMQLWNWRGLDV"
     gene            complement(818..1996)
                     /locus_tag="VFO72_03055"
     CDS             complement(818..1996)
                     /locus_tag="VFO72_03055"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9478295.1"
                     /translation="MTGGKTNAAANGNGRYPHFKTLTPTPGNKNGKAKPILITGGAGF
                     VGSNLAHRLLSAGHRVRLLDNLSRSGVARNLHWLIETHGDMVDIEVPDIRNFSIVKQA
                     VKDASQIFHFAAQVAVTSSLVDPIADFEINARGTLNVLEAIRHTDNPPPLVFTSTNKV
                     YGNLGDLQFTNGSTRYVPVDPFVRERGIAENRPLDFHSPYGCSKGAADQYVTDYARTY
                     RLPALVLRMSCIYGPHQYGNEDQGWVAHFLIRSITGQPITIYGNGKQVRDILFVNDLV
                     DAFLLAQQHMPRLSGNAYNIGGGPANMISLLELLDLITELHGGDLSIEFEDWRAADQR
                     YYVADTSKFAGVTGWRPKVGVREGVRRLYEWLMTSSQHFSEVPVGAGEMVRRVEARKI
                     "
     gene            2208..>3012
                     /locus_tag="VFO72_03060"
     CDS             2208..>3012
                     /locus_tag="VFO72_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478296.1"
                     /translation="MNNADDIDRWTEAMLRGDFAAAWSTSDALLHRSRTQDRRSTPRH
                     LQNIWDGTPVAGKRVLVRCYHGLGDTIQFIRYAPLLKRVPAEVIVWVQPSLIPLLSSV
                     EGIDRLMPLHDGEPDIDFDVDVEVTELPYLFRTTLENIPQVPYLQVDPAPLARVDDKI
                     RAGLIWQSGDWKPARSIPFPALQPLAEVSGIQWHLLQRNPHEAGWDGQFGMISGGDNP
                     LHDAHMMRALDLVISVDTMTAHLAGALGTNTWTLLPHNPDWRWMTDREDS"
BASE COUNT          651 a          865 c          842 g          654 t
ORIGIN      
        1 acgtcgagcc cgcgccagtt ccagagctgc atgtttactt gtcttggacc atcctggtga
       61 taacccgcaa taacgagccg tccacgttcg ctcgtgagtt cggtcgcaag atcgagcgat
      121 tcctgtgtac cggctgtttc gattacacaa tcgcagccga ggtcgtttgt aagccgtttc
      181 accgcttcca acgcctgact gctctcaata gtggccgcag cgccgaactg ctccgccata
      241 gtcaacgacg aacgccggcg cgagattgca aacacttttg cgcgtgcgtt gactgcaagc
      301 tgtgtcaata acgtgccgag aaaaccaata cctacgatcg cgacagtctc ttcgggcaag
      361 atcgtggaac gccggaaaat gttggcggca caaccgagag cttcagcggg acacggttgt
      421 tcgtccagtg aagccgggag gcggaccacc tgagacgcag gcgcaaagtc gtaagtcgca
      481 aaaccgtgcg acgaaagcat cgcgacccga tctcccggcg ccggatcaac tacgcccaat
      541 ccgattgcat cgatgtagcc ccaggcttcg tgacccggtg cgcccggctc gagcggatac
      601 tcgaaccacg gtttgccctg ccacactgga agattcgaag cgcaaacacc gcagccttca
      661 atgcgcacgc gcacctcgcc ctcggcgggt tctcggaaat cgatctcacg cagatcgatc
      721 tgcgtgggcg ctgatattac tgccgcccgc ccgcgcacag ccggaactgc tgctgcgacg
      781 cgcttgctgc ttgccgttga ttgcactaac ccctgcatta gattttccgg gcttcaactc
      841 tgcgcaccat ttcgccggcg ccaactggca cctcggagaa atgctgacta ctcgtcatca
      901 accattcgta gagacgtctg acgccttcac gaactcccac cttcggccgc caaccagtca
      961 cgcccgcaaa cttgctcgtg tccgcgacgt agtaacgctg atccgccgcg cgccagtctt
     1021 caaattcaat cgaaagatca ccgccgtgta gttccgtgat cagatccaac aactctaaca
     1081 ggctgatcat atttgccggc ccaccaccga tgttgtaagc gtttccggaa agtcttggca
     1141 tgtgctgttg cgccagcaaa aacgcgtcga ccagatcgtt aacaaagaga atgtcgcgga
     1201 cctgcttgcc gttgccgtag atcgtgatcg gctggccggt gatggatcga ataaggaagt
     1261 gtgctaccca gccctggtcc tcgttgccgt actggtgcgg cccgtaaatg cagctcatgc
     1321 gaaggacaag cgccggaagg cgataggtgc gcgcatagtc ggtcacgtat tggtccgcgg
     1381 cgcctttcga acagccgtag ggactgtgaa agtcgagcgg gcgattctca gcgatgcccc
     1441 gttcgcgaac gaaggggtcg acaggcacgt atcgagtgga gccgttcgtg aactgaagat
     1501 cgccgagatt accgtaaacc ttgtttgttg aagtaaaaac caacggcggt ggattgtctg
     1561 tgtggcgaat cgcctcgagc acattgagtg tgccacgcgc attgatctca aagtccgcga
     1621 tgggatccac gagactcgaa gtcactgcca cctgcgccgc gaaatgaaag atctgcgaag
     1681 cgtctttgac tgcctgtttg acaattgaga agttgcgaat gtcgggaacc tcgatatcga
     1741 ccatgtcgcc gtgcgtctcg atcaaccaat gcaagtttcg tgcgacgccg gaacgtgaaa
     1801 gattgtcgag caaacgcacg cggtgaccgg cagaaagcaa ccggtgcgca aggttcgagc
     1861 cgacaaaacc ggcgccgccc gtgatcagaa ttggtttcgc tttaccgttc ttgttgccgg
     1921 gcgttggagt taatgttttg aagtgtgggt agcggccatt tccgtttgcg gcagcgttcg
     1981 tttttccacc tgtcatcttc tccaccttcc tacgaagctg tgcgcggtgc tcagccactg
     2041 ctattcatga gtcccccgag gtgaacgaaa agtacaggtc gtggatcgtg aattcggtac
     2101 gttggcgtac cgcgacttta taagtgtaag taccgtgaaa aaattgttag gaagttgctg
     2161 ctgtcgatca tcagcaggga gtagtcgcgc tggtatgctg tccgctgatg aacaacgcgg
     2221 acgacattga tcgctggaca gaggcaatgc tacgtggtga ttttgcggct gcgtggagta
     2281 ccagtgatgc gctgctacat cgatcgcgca cgcaggatcg tcgttctact ccgcgacact
     2341 tgcaaaacat ttgggacgga acaccggtcg caggaaagcg agtgctggtt cgttgctatc
     2401 acggactggg tgacacgatt caattcatcc gctatgcacc tttgttaaag cgcgtgccgg
     2461 cggaggtgat tgtttgggtt caaccgtctt tgatcccttt gctcagcagc gtcgagggaa
     2521 tcgatcgatt gatgccgttg cacgatggtg agccggacat cgacttcgat gtggatgtcg
     2581 aagtcaccga gttgccttat ctgtttcgca caacgcttga gaacattcct caggttccat
     2641 acttgcaggt tgaccccgcg ccactcgcgc gtgtggacga caaaatccgc gccggtttga
     2701 tctggcagtc aggcgactgg aaaccggccc gctcaatccc ttttcccgcg ttacaaccgc
     2761 tggcagaagt ttccggaatt cagtggcacc tgctgcaacg caacccccac gaggcaggtt
     2821 gggatggtca gttcggcatg atttcaggcg gcgataaccc actccacgat gcacacatga
     2881 tgcgcgcact cgacctcgtg atctccgtcg acacgatgac cgctcacctt gcaggagctc
     2941 tcggaacaaa cacgtggacg ctgctcccac acaatcccga ctggcgctgg atgacggatc
     3001 gtgaagacag tc
//
LOCUS       DASTEY010000173         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_382974, whole genome shotgun sequence.
ACCESSION   DASTEY010000173 DASTEY010000000
VERSION     DASTEY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382974"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFO72_03065"
     CDS             complement(<1..479)
                     /locus_tag="VFO72_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9478297.1"
                     /translation="MIQPSERAVNFNYAIRNVVRAAEALERKGRRVIYLNIGDPQAFG
                     FRPPDSVIEAVNRAVRDKFSGYSHSSGLLEAREAIARYATRLGSPTETKDVIVTAGAS
                     EAADLTLTALVNPGDEVLLPAPGYPIYPAIVGKLGAQIRYYHLQPQDGWHLSVDEVRS
                     "
     gene            500..907
                     /locus_tag="VFO72_03070"
     CDS             500..907
                     /locus_tag="VFO72_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478298.1"
                     /translation="MREIGSPEQEIYLTKDRGDGIVWAFEVGDKGPQLFTLLVREIDK
                     TQAKTDCLKVMGHFAGQLEPGAVRELDPESQLLTDIGLAEGIDKTSALREVGDPRRAF
                     EGAAPNGIQTNLQSLFTAAVVHIMETENSAYQD"
     gene            956..2188
                     /locus_tag="VFO72_03075"
     CDS             956..2188
                     /locus_tag="VFO72_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET9478299.1"
                     /translation="MFKPQAEILPLAEIRKHFPALERVHDGYPVAYFDGPGGTQVPRY
                     VVEQMSDYLYHHNANTHWAYPTSAETDAAIEHTREVCAGFLNASASEIVFGANMTTLT
                     FHLSRALGLKYSAGDEIVVTELDHHANIDPWRRLAVERGVTIRTVRFDTATGQLNYEE
                     LERFIGPKTRVVAIGAASNALGTINDLTRVIELAHSAGALVFVDAVHYTPHALIDVRQ
                     LDCDFLGMSAYKFYGPHVGILFGKAALLGEIDFPKVVPAPDSVPENAETGTPNQEGMV
                     GARAAIEFLASLGHKTTMRENLQDVFAETHARNARLFARLWDGLSSIPGVTLYGPPPH
                     APRTPTLVFTIEGRTATEAARRLAEKGLFLSHGDFYARTVVQRFGLAPEGFIRAGCAC
                     YTSEEEIERLIASVADLC"
     gene            complement(2201..3205)
                     /locus_tag="VFO72_03080"
     CDS             complement(2201..3205)
                     /locus_tag="VFO72_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478300.1"
                     /translation="MKRYRLCFLGFGNVGRALARLLVAKSAELRDNYGIEWEITGVAT
                     RRIGWRASDRAIDVVALLAGNYDALTQNSISEWLAVAKPDVVFETTSLNPETGQPAID
                     YLRASLQAGAHTITANKAPIVYAYDELNEIAVAQGKRFLFESTVLDSAPVFSLFRETL
                     PAVKIRGFSGVFNSTTNIILETMEAGRSFAEGVKTAQELGVAETDPSHDVDGWDSVMK
                     VCAVARVILKTPLMPGAVRCEGIRGLTHAVLQAARAEGKPFKLLARATVNADGSVTAS
                     VRPEQIATNDPLGNVRGTSLAIHFELDTIPGLTIVSHRPNLQSTAYGLLADLVNAATD
                     "
     gene            3221..>3630
                     /gene="thyA"
                     /locus_tag="VFO72_03085"
     CDS             3221..>3630
                     /gene="thyA"
                     /locus_tag="VFO72_03085"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET9478301.1"
                     /translation="MEQYHDLLRSILENGVEHRDRTGVGTISHFGFQTRFDLREGFPI
                     VTTKRVPFRWVAEELFWFLSGDTSETNLRAKGVDIWAEWADDEHTRKFGRPAGDLGPV
                     YGYLWRSFGGDYPQKNGVDQIAKVLDLIRTNPDSR"
BASE COUNT          749 a         1035 c         1087 g          759 t
ORIGIN      
        1 gaccgcactt cgtcgaccga aagatgccaa ccatcttgcg gctgcaagtg atagtaacga
       61 atctgcgctc ccaacttgcc gacaatcgct ggatagatcg ggtaacccgg agcaggcaac
      121 aacacttcgt cacccggatt gaccagcgcg gtaagcgtca gatcagcggc ttcggaagct
      181 cctgctgtaa cgatcacgtc tttcgtctcg gtcggggagc ccagtctggt ggcgtaacgc
      241 gcaatcgcct cgcgcgcctc gagcaaaccc gaagaatggg aatagcctga gaacttgtcg
      301 cgcacggcgc gattaaccgc ttcgatcact gagtcgggcg gtcgaaagcc aaatgcttgc
      361 gggtctccga tgtttagata gatgactcgt cgtcccttac gttccagggc ttcggcggcg
      421 cgaacgacat tgcggatggc gtaattgaaa ttgacggctc gttctgaagg ctgaatcatg
      481 tccggcaatt gtaaaagatt tgcgagaaat tggctcgccg gaacaggaga tttatttaac
      541 gaaggaccgc ggtgacggga tcgtgtgggc ctttgaggta ggcgataaag gaccgcaact
      601 gttcacgctg ctcgtccgtg agattgacaa aacgcaggcc aaaaccgact gtctgaaagt
      661 gatgggccac ttcgccggtc agctcgagcc aggcgccgtc agggagctgg acccggaaag
      721 tcagcttctc accgatatag gtctcgccga gggcatcgac aaaacatccg ccctcagaga
      781 ggtcggtgat ccgcgcaggg cgtttgaggg tgccgcccca aacggcatcc agacaaacct
      841 gcaatcgctc ttcactgcgg cggtcgttca tattatggaa acggagaaca gtgcttatca
      901 ggactgaggt ccggccactg tcaggcgacg aactgatttt atattatggc gtcgcatgtt
      961 taaaccccaa gcagaaattc tgcctctagc cgaaatcaga aaacactttc ccgccctcga
     1021 gcgagtacac gatgggtatc cggtcgccta cttcgacggc ccgggcggta cgcaggtccc
     1081 gcgttacgtc gtcgaacaga tgagcgacta tctttaccat cacaatgcaa acacgcattg
     1141 ggcctacccg accagcgccg agactgacgc cgccatcgaa cacacccgcg aggtttgcgc
     1201 cggcttcctg aacgcgtcgg ccagcgagat tgttttcggg gcgaacatga cgactttaac
     1261 gtttcacctg tcgcgagcgc tcggcctcaa gtattcggca ggcgatgaga ttgtcgttac
     1321 ggagctggat caccacgcga acatagaccc gtggcggcgg cttgccgtcg aacgcggtgt
     1381 gactattcga accgtgcgat tcgacacggc caccgggcaa ctcaactacg aagagctcga
     1441 acgttttatt ggaccaaaga cgcgtgttgt cgcgatcggc gccgcctcaa acgcgctcgg
     1501 cacgatcaac gatctcacgc gagtgatcga gttggcccac agcgccggcg cgctggtgtt
     1561 tgtcgatgcg gttcactaca cgccgcacgc gctgatcgac gtgcggcaac ttgactgcga
     1621 ctttctgggg atgtcggcct acaagtttta tggaccacac gtcggaatac tttttggtaa
     1681 agccgcgctg ctgggcgaga tcgactttcc gaaggtcgtg ccggcccctg attccgttcc
     1741 cgagaacgcg gagacgggca cgccgaatca ggaaggaatg gtcggtgcgc gcgcggcgat
     1801 cgagtttctc gcctcgctgg ggcacaagac gacgatgcga gaaaacctgc aagatgtatt
     1861 tgccgagacg cacgcgcgta atgctcgact gttcgctcgt ttgtgggacg gcttgagctc
     1921 gataccgggt gtgacacttt atggtccgcc accgcacgct ccgcgaacgc cgacgcttgt
     1981 tttcacgatc gagggtcgca ctgcgacgga agccgcgcgc cgtttggccg agaagggtct
     2041 gtttctttct cacggcgact tctacgcccg gaccgtagtg caacggtttg gactcgcacc
     2101 ggaagggttc atccgtgcgg gctgcgcgtg ttacacaagc gaagaggaga tagaacggct
     2161 gattgctagc gtcgccgatc tgtgttaatc cgtggccgtg ttaatccgtg gccgcattga
     2221 ctaaatccgc cagcaacccg tacgcggtac tctgtaagtt cggccggtgc gacacgatcg
     2281 taaggccggg aatggtgtcc agttcgaaat gaatggcgag cgaagtgccg cgcacgttgc
     2341 cgagcggatc gttcgttgcg atctgttcgg gtcttactga cgcagtgact gagccgtcgg
     2401 cattaacagt ggcgcgggcc aatagcttga acggctttcc ctccgcacgc gcggcctgca
     2461 aaacggcatg ggtcagcccg cgtatccctt cacatcgcac ggcgccgggc atcaagggcg
     2521 tcttcagaat cacgcgcgcg acagcacaga ctttcattac cgaatcccaa ccgtcgacgt
     2581 cgtgactcgg atcggtctcc gcgacgccaa gttcctgcgc cgtcttgacg ccttcagcaa
     2641 aacttctgcc tgcctccatc gtttcgagaa tgatgttggt ggttgagttg aacacgccgc
     2701 tgaagccacg gatttttacg gcgggcagtg tctcgcgaaa cagcgagaaa actggtgcgc
     2761 tgtcgagcac ggtcgattcg aatagaaatc gtttgccttg cgcgacggca atctcgttca
     2821 gttcgtcgta ggcgtaaacg atgggcgcct tgttggccgt gattgtgtgc gcgcccgctt
     2881 gcagcgaggc gcggaggtag tcgatcgccg gctggcctgt ttcggggttg agtgaggtcg
     2941 tttcgaaaac gacgtcgggc tttgctaccg cgagccactc gctgatgctg ttttgcgtta
     3001 gcgcgtcgta gtttccggcg agcaacgcaa cgacgtcaat cgcccgatca ctcgcgcgcc
     3061 atccgatgcg acgcgtcgcg acgcccgtga tctcccattc gatgccgtag ttgtcgcgca
     3121 actccgcgga cttcgccacc agcagtcgcg ccagcgcgcg gccaacattt ccaaatccca
     3181 ggaagcacag ccggtaacgt ttcatgtatg atggagcccg atggaacaat accacgatct
     3241 gctgcgatcg attctagaga atggcgttga gcatcgggac cgcaccggcg tcggcacgat
     3301 ttcgcacttc ggctttcaaa cacgtttcga tctgcgcgag ggtttcccga tcgtgacaac
     3361 caaacgcgtg ccgttccgct gggtggcaga agagttgttc tggtttctat cgggtgatac
     3421 gagcgaaaca aacctgcgcg cgaagggtgt cgatatttgg gccgagtggg ccgacgacga
     3481 acacacgcgc aagtttggcc ggccggcggg agatcttggg cctgtttacg gttatctttg
     3541 gcgctcgttc ggtggcgatt atccgcagaa gaacggtgtc gatcagatcg cgaaagtgct
     3601 cgacttaatc cgtacgaatc ccgattcgcg
//
LOCUS       DASTEY010000174         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_201605, whole genome shotgun sequence.
ACCESSION   DASTEY010000174 DASTEY010000000
VERSION     DASTEY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201605"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="thrS"
                     /locus_tag="VFO72_03090"
     CDS             <1..1344
                     /gene="thrS"
                     /locus_tag="VFO72_03090"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9478302.1"
                     /translation="GDASQPQMQRIYGTAFFTQEELDAWLKQRAEAEKRDHRRLGREL
                     DLFSIQEEYGQGLIFWHPKGGAIRKEMEDYLRDQLVRRGYGLVFTPHIAKRELWFTSG
                     HESNYADSMFSPMEFEEQEFRIKPMNCPFHIGIYKSAQRSYRDLPLRYAELGTVYRAE
                     LSGTLHGLTRVRGFTQDDAHLFCTPENVHAEILGCIDFAFNMYEVFGFKDIKVELSVR
                     GDDPNAHYLGSDEQWEWAESALIEALNAKQIPYQRIEGEAVFYGPKIDIRVEDAIGRN
                     WQLTTVQFDFNLPERFQLEYIGEDNKPHRPVMIHRALCGSMERFFGVLIEHYGGVFPM
                     WLAPVQVAVLPITDRINDYAEEVARELRNAGFRVETNLRSEKIGAKIRDAQLQKVPFM
                     LVLGDREMEQRAVAVRERARGDIGSMSLEEFKEMARKLVETRALTNNLKTQEVQQ"
     gene            1404..1931
                     /gene="infC"
                     /locus_tag="VFO72_03095"
     CDS             1404..1931
                     /gene="infC"
                     /locus_tag="VFO72_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9478303.1"
                     /translation="MNERIRVPEVRVIGEEGEQLGVMDVRDAIRAARDKGLDLVEVAP
                     NADPPVCRIIDFGKYQYEAKKKANEAKKKQVTITVKEVKFRPGTDDHDYNYRMKHARE
                     WLQDGDKVKATIWFRGREMTHRELGARILEKLEQDLADVGEVEARPRMEGNQMFIIFG
                     PKRHKGGGKDTASPQ"
     gene            2012..2215
                     /gene="rpmI"
                     /locus_tag="VFO72_03100"
     CDS             2012..2215
                     /gene="rpmI"
                     /locus_tag="VFO72_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET9478304.1"
                     /translation="MPKLKTHKGAAKRFRLTATGKIKRGHSHARHILTKKTNKRKRHL
                     DIDVLVSKADQERVMAMLPYGRD"
     gene            2327..2707
                     /gene="rplT"
                     /locus_tag="VFO72_03105"
     CDS             2327..2707
                     /gene="rplT"
                     /locus_tag="VFO72_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9478305.1"
                     /translation="MPRAKRGNKRLERRKKILKLAKGYRGTKSKLYRSAKESVERGLN
                     FAYTGRKLKKRDFRSLWIVRIGAAARLNGLNYSQLMHGLKLASIELDRKVLADLAVHQ
                     PESFAEVASQAKNALENEQRNKAA"
     gene            2727..3428
                     /locus_tag="VFO72_03110"
     CDS             2727..3428
                     /locus_tag="VFO72_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478306.1"
                     /translation="MKITTLLLTFLLGATIALAQGTPAPPRLPAIMDKLVGNYEGTVK
                     GQTEQVWLELMPEGGNMGRLKVGEKTYDLTEGLIGDGTFSFVLKDGKLTGKIDGDMLT
                     ADLWVGSEKKAIELKRVPAAAAATPAAATPAADAPINLNGQWEAVADANGQPVPFLLT
                     LKVEGETVTGGSSSQLGGATIKDGLWKEGKLMFSLEGSSGTIALIATVVDGKLSGEFD
                     YAGQLQGKWVAVKKN"
     gene            3434..4537
                     /gene="pheS"
                     /locus_tag="VFO72_03115"
     CDS             3434..4537
                     /gene="pheS"
                     /locus_tag="VFO72_03115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9478307.1"
                     /translation="MTNSQTEQTPAQQVEAARKAFEQEFERFARFSDSAELARLDLET
                     AIAEERALAEVRTRHFGKKSVLAAAKKSIGRVAPEERPAFGQLVQSTEAAIVRAIEQA
                     EQNLKQYIEQERTARESIDVTLPGRRPPYGHRHPITLLRERIEDIFVALGYVIEDGRE
                     IETDFYNFSALNIPENHPARGEADTFYTTEGFALRSQTSTVQIHAMQRRGVPIRMIAP
                     GRVFRRDTPDPTHNPMFYQVEGLCVDRGITMAHLKGTVTEFLRRMFGPDTVTRFRPSY
                     FPFTEPSAEFDFSCFKCKGAGCRICKNSGWIELGGSGMVHPNVLRAVDVDPQVYSGFA
                     FGLGIDRMCALMYELDDIRLLFENDVRFLEQFE"
     gene            4549..>5655
                     /locus_tag="VFO72_03120"
     CDS             4549..>5655
                     /locus_tag="VFO72_03120"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HET9478308.1"
                     /translation="MFIGYDWLKELTDTNFSPDELRERLTRVGLAIDAVESHNSDFVL
                     DVEVPSNRPDCLSHVGIAREVAVIENKQIRLPQVEVSKTEGRSSELTSVEIKDADLCP
                     RYAARLVRGVRIGPSPEWLAKRLESIGQRPINNVADITNYVLHELGQPLHAFDFQKLS
                     GQRIVVRRAKAGEKLTTLDGVERTLTNEMLVIADADRPVALAGIMGGEESEISSATTD
                     VLIESAYFEPHSVRRTARALGMDTEASRRFERGADCEGVLRAQERCVQLICEIAGGVA
                     TEDWIDIYPRPFVPRVSSLRPERVPELTSVRIDYGDIVRILSGLGFKIVDDSREKLTV
                     QVPSWRVDVEQEEDLVEEVARHTGFEKIGSELPPS"
BASE COUNT         1339 a         1461 c         1709 g         1146 t
ORIGIN      
        1 ggcgacgcgt cgcagccgca gatgcagcgc atctacggca cggcgttctt cacgcaggaa
       61 gagttagacg cgtggctgaa acaacgcgca gaggcggaga agcgcgatca tcgacggctg
      121 ggccgcgaac tagatctctt ttcaattcag gaagagtacg gccagggact gatcttctgg
      181 cacccgaagg gcggcgccat tcgcaaggag atggaagact atctgcgcga tcagctggtc
      241 aggcgcggtt acggtctggt gtttacgccg cacattgcga aacgcgaact gtggtttacg
      301 agcggtcacg aaagtaatta cgccgattcg atgttttcgc cgatggagtt tgaggagcag
      361 gagtttcgca tcaagccgat gaactgcccg tttcacatcg gcatttacaa gtcggcgcag
      421 cgttcgtatc gagatcttcc gttgcgttac gcggagctgg ggacggtcta tcgggctgaa
      481 ctatcgggta cgttacacgg cctgacgcgc gtgcgcgggt ttacgcagga cgatgcccac
      541 ctcttctgca cgccggaaaa cgtgcacgcg gagatactcg ggtgcatcga ctttgctttc
      601 aatatgtatg aagtttttgg cttcaaggat atcaaggtcg aactttctgt tcgtggtgac
      661 gatccgaacg cgcactatct cggatcggac gagcaatggg aatgggcaga gtccgcgttg
      721 atcgaagcct taaacgccaa gcagattccg tatcagcgaa tcgaaggcga ggcagtgttc
      781 tacgggccga agatcgacat tcgcgtcgag gacgcgatcg ggcgcaattg gcagttgacg
      841 acggtacagt ttgatttcaa tttgcccgag cgttttcaac tcgagtacat cggtgaagac
      901 aacaaaccgc atcgcccggt gatgatccac cgcgcgttgt gtggttcgat ggaacggttc
      961 ttcggtgttc tgatcgagca ctacggcggc gtgttcccaa tgtggttggc gccggtgcag
     1021 gttgcggtgc tgccgattac ggatcgaatt aacgactacg ccgaggaagt agcgcgagaa
     1081 cttcgcaacg ccggtttcag agtagaaacg aacttgcgta gcgaaaaaat aggcgcgaag
     1141 attcgcgacg cgcagttgca gaaggtgccg ttcatgcttg tgctcggcga tcgcgagatg
     1201 gagcagcggg cagttgccgt gcgcgaacgc gctcgtggcg acatcggttc gatgtcgctc
     1261 gaagaattca aggagatggc gcgcaagttg gttgaaacgc gcgccttgac taataaccta
     1321 aaaacccagg aggtgcagca atagctacag gctttcgcgg tcgcggtcag cgtcctgata
     1381 atcggcgtct gccgccggtt cgaattaacg agagaattcg cgtacctgaa gtacgcgtga
     1441 ttggcgagga aggagaacaa ctgggcgtga tggatgtgcg cgatgccatc cgtgccgccc
     1501 gcgacaaagg tttggatttg gtcgaagttg ctcccaacgc cgatccgcct gtctgccgga
     1561 tcatcgattt tggaaaatac cagtacgaag cgaagaagaa ggcaaacgag gcgaagaaaa
     1621 agcaggtcac gatcactgtt aaagaagtga agtttcgacc gggaaccgac gatcacgact
     1681 acaactaccg catgaaacac gcacgcgagt ggttgcagga cggcgacaaa gtgaaagcga
     1741 cgatctggtt tcgcggccgc gagatgacgc accgcgagtt gggcgcgcgc atcctggaaa
     1801 aactggaaca ggacctggcg gacgtcggcg aggtcgaagc aaggccgagg atggaaggga
     1861 accagatgtt cattatcttc ggaccgaaga gacacaaggg cggcgggaaa gacaccgctt
     1921 cgccgcaatg aatgatcgat tgcgattgcg attgccgatt gccgattgcc gatttcaaat
     1981 cgtcaatcaa aaataactaa aggaataatc aatgccaaaa cttaagacac acaaaggtgc
     2041 agcgaagcga tttcggctga ccgcgactgg caagattaag cggggccact cgcatgcacg
     2101 acacatactg acgaagaaga ccaataagcg gaagcgtcac ctggatattg acgtgctcgt
     2161 ttccaaggcc gaccaggaac gagtgatggc gatgttgccg tacggccgcg attagtcatt
     2221 ttcgcttgcc gattttcgat tgtcgattgt ggaggacgtc gtccgccact cttcaaatcg
     2281 gcaatcggca atcgaaaatc aaaaattctt atagggaggt catcatatgc ccagagcaaa
     2341 acgtggcaat aagcgtctcg aaagacgcaa gaagatactg aaactagcga aaggttatcg
     2401 cggcacgaag tcgaagcttt atcgatcggc gaaggagtcg gtcgagcgcg ggttgaattt
     2461 cgcgtacacc ggccggaagt tgaagaagcg tgatttccgc agtctgtgga tcgtgcgcat
     2521 cggcgccgcc gctcgcttga acggtttgaa ctattcgcaa ctgatgcatg gtttgaaact
     2581 cgcgagcatc gagctcgatc gcaaagtgct ggccgatttg gcggttcatc agcccgaatc
     2641 gtttgccgaa gtggcgtctc aggcgaagaa cgcgctcgag aacgagcagc gcaataaggc
     2701 cgcgtaattt tgaaaggacc aaagcagtga aaataacgac acttctactc acgtttttgc
     2761 tcggcgccac catagctttg gcgcagggaa ctcccgctcc acctagactt ccagcgatta
     2821 tggacaagct agtcggcaac tacgaaggaa ccgtgaaagg acagaccgaa caggtttggc
     2881 tggagctgat gcccgaaggt ggcaacatgg gccgtttgaa ggtgggcgaa aaaacatatg
     2941 accttactga aggattgatc ggtgacggaa ctttctcgtt cgttttgaag gacggaaaac
     3001 taaccggcaa aatcgatggc gacatgctca ctgctgactt gtgggtgggc tctgagaaaa
     3061 aagcgatcga actgaaaaga gtcccggctg cggccgcggc aactccggcc gccgcgactc
     3121 ccgccgccga cgctccgatc aacttgaacg gacaatggga agcagtggcg gacgcgaatg
     3181 gtcagccggt tccgtttctc ttaacgctca aagttgaagg cgaaactgtt accggcggca
     3241 gcagttcgca acttggcggt gcaaccatca aagatggttt gtggaaggaa ggcaagctta
     3301 tgttttccct cgaaggctca agtggcacca tcgcgttaat cgcaactgtc gttgacggaa
     3361 aactttccgg tgagttcgat tatgccggac agttgcaggg caagtgggta gcagtgaaga
     3421 agaactagtt tgaatgacca atagtcaaac ggaacaaacg cctgctcagc aggttgaagc
     3481 ggcccgcaaa gcgttcgagc aagagttcga gcgctttgcc cgctttagcg attctgcgga
     3541 actggctcgc ctcgacttgg aaaccgcaat cgccgaagag cgtgcactgg ccgaagtgcg
     3601 tacgcgccat tttggtaaaa aaagcgtgtt ggcagccgcc aagaagtcga ttggccgcgt
     3661 tgcgccggaa gagcgtccgg cgttcgggca gttggtccag tcgaccgaag ctgcgatcgt
     3721 tcgcgcaatc gaacaggctg aacagaacct caagcagtac atcgaacagg aacgcaccgc
     3781 gcgcgaaagc atcgacgtca cgctgccggg ccgccgtccg ccttacggcc accgacatcc
     3841 gataaccctc ttgcgcgaac gaatcgagga catctttgtg gccctcggct acgtcatcga
     3901 agacggacgc gaaatagaaa cggatttcta caacttctcc gcgctcaaca tcccggaaaa
     3961 ccatccggca cgcggcgaag ccgacacgtt ctacaccacc gagggctttg cgctgcgctc
     4021 gcaaacatca acggtgcaga tccacgccat gcagcgacgc ggcgtaccga ttcgcatgat
     4081 cgcgcccggc cgcgtatttc ggcgcgatac gcctgatccg acgcacaacc caatgttcta
     4141 tcaggtcgaa ggcttgtgcg tggaccgcgg gatcaccatg gcacatttga aaggaacggt
     4201 gaccgagttt ttgcgacgca tgttcgggcc cgacactgtc acgcgctttc gcccgtctta
     4261 ctttccgttt acggaaccga gcgcggagtt tgatttcagt tgtttcaagt gcaaaggcgc
     4321 gggctgccgg atctgcaaaa actcgggttg gatcgagctc ggcgggtccg ggatggtcca
     4381 tccgaacgtg ttgcgtgcag ttgacgtcga tccgcaggtg tattccgggt tcgcgtttgg
     4441 tctgggcatc gaccgcatgt gcgcgctgat gtacgaactc gacgacatac ggctgttgtt
     4501 tgagaacgac gtgagatttc tggagcaatt cgagtaggcc actgaaaaat gttcataggt
     4561 tacgactggc ttaaagaact tacagacaca aacttcagcc cggacgaact gcgcgagcgg
     4621 ttgaccaggg tggggctcgc catcgacgcc gtcgagagcc acaactctga cttcgtgctg
     4681 gacgtcgaag ttccgtcgaa cagacctgat tgtcttagcc acgtcggcat cgcgcgcgag
     4741 gtggcggtta ttgagaacaa acagatccgt ctgccgcagg tggaagtctc gaaaactgag
     4801 ggtcgttcta gtgagctgac ttcagttgaa ataaaggacg cggatttgtg cccgcggtac
     4861 gcagcgcgtc tggtgcgcgg cgtaaggatt ggcccctcgc ccgagtggct ggcgaagcgt
     4921 ttggagtcga ttgggcagcg tcctattaac aacgttgccg acatcacgaa ttacgtcttg
     4981 cacgagttgg gccagccgct gcacgccttc gattttcaaa aactttccgg tcagcgcatt
     5041 gtcgtacgcc gcgccaaagc aggcgagaag ctcacaacgc tcgacggcgt cgaacgcacg
     5101 cttacaaacg agatgctcgt tatcgccgac gcggatcgac cggtcgcact cgccggcatc
     5161 atgggcggcg aagagtcgga gatctcgtct gcgacgaccg atgtactgat cgagagcgcc
     5221 tatttcgagc cgcattctgt gcggcgcacg gcgcgagccc tcggtatgga cactgaggcc
     5281 tcgcgcagat tcgagcgggg cgccgactgc gaaggggtgt tgcgcgcgca ggagcgttgc
     5341 gtgcaactca tctgtgagat tgcaggaggc gttgcaactg aggactggat tgatatttat
     5401 cctcgcccct ttgtcccccg cgtatcgagt ctccgtccag agcgcgtgcc tgaactcaca
     5461 tcagttcgca tcgactatgg agacattgtt cgaattctta gcggtctggg gttcaaaatc
     5521 gtagacgact cgcgcgaaaa gttgacggtt caggtgccgt cgtggcgtgt agacgtcgaa
     5581 caggaagaag atctggtcga agaagtcgcg cgacacaccg gctttgaaaa gatcggttcg
     5641 gagttgccgc cctcg
//
LOCUS       DASTEY010000175         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1619053, whole genome shotgun sequence.
ACCESSION   DASTEY010000175 DASTEY010000000
VERSION     DASTEY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619053"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFO72_03125"
     CDS             <1..406
                     /locus_tag="VFO72_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET9478309.1"
                     /translation="GDLPQFVDFAYVANVARVNAAALATLAYAPSRPKGVTIVSRLSD
                     DTELRWEANNEPDLAGYEIVWRDTTAPVWTNSRPVGKVTTFTLKGMSKDNFFFGVRAV
                     DNDGNRSPVTFPRPMTRTQPPNRRTTTSSGNP"
     gene            452..2155
                     /locus_tag="VFO72_03130"
     CDS             452..2155
                     /locus_tag="VFO72_03130"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HET9478310.1"
                     /translation="MRFSKKRLIAAMVIAAITASQTLVASADTQKSAKKRRLTEEQQI
                     IHVLNRLGFGARPGDVQRVKSFGLENYINQQLNPEKITDTVAENKVKDIGVLKLTTAE
                     LYAKFPQPGQLLRQLQARGMLPADLQREPQTNPPNPQDERYRKVIDEYYRQNGLQRPQ
                     RIMAELHASRILRAVYSERQLQEVMVDFWTNHFNVFAGKGADRWLLPAYDRDTIRPNA
                     MGKFSQLLLATAQSPAMLFYLDNFQSVSPNANRGYARQPLNAQRRRGINENYARELME
                     LHTLGVDGGYTQKDVQEVARCFTGWTIFQPRGGAAAANAMMGEAGRRNAGTFFFNSRM
                     HDDGEKTVLGHKISAGGGFKDGLTVIDILARHPSTAKFIATKLVRHFVSDTPPAALVE
                     RVAAAFAKSDGDIRETLKAIFFSSEFNSAEAYRVKIKRPFELAVSAIRALGADTNGGP
                     GTHQWIARMGEPLYGFQTPNGYSDAAESWVNTGGLLERMNFGLALAGNRIPGTRVDLA
                     KLAGQEKSKLIDESLKLMVAGEVSAATRETLMKQINESSADTAAKVVGLILGTPEFQR
                     Q"
     gene            2515..3780
                     /locus_tag="VFO72_03135"
     CDS             2515..3780
                     /locus_tag="VFO72_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET9478311.1"
                     /translation="MNRRFFLKSGSIALASVGMSLAAPAFLQRAIMAHNATGGRRKTL
                     IAIFQRGAVDGLNMIVPFGESSYYDARPTIAIPKPDSANAEAAISLDGFFGLHPSMSS
                     LKPLWDSKRLAVVHASGSPDNTRSHFDAEDYMESATPGVKSTPDGWLNRYLQSKKDEQ
                     RSLFRAVSMTQQMPRAFEGRAPVLAISNLSNFAIRAGKSSASVQDGFEAIYSRDKNET
                     LSATGRETLEAINYLKEVNPAQYKPENGAQYPRGAFGNSLLQIAQLIKAGVGLEIAFT
                     DIRGWDTHVNQGNSRGQLANLLQQFSSGLAALYMDLGQRMDDVVVLTMSEFGRTVREN
                     GNRGTDHGHANAMMVIGNSVRGGQVYGRWPGLKSDQLYEGRDLALTTDFRDVFGEVAA
                     RHLRTTDLKSVFPGYSTGPAKFKGLFRNG"
BASE COUNT          899 a         1086 c         1142 g          787 t
ORIGIN      
        1 cggcgacctg ccgcagtttg tcgactttgc ttacgtcgcg aatgtggcgc gcgtgaatgc
       61 ggcagcgctg gcgacgctgg cctacgcgcc ttcgcgcccc aaaggcgtga cgatcgtttc
      121 tcgtttgtcc gatgacacgg aattgcgttg ggaagccaac aacgaaccgg atctggcggg
      181 ctacgaaatc gtctggcgcg acacgactgc gcccgtgtgg accaattcgc ggccggtggg
      241 aaaagtcacg acgtttacgc tgaaaggcat gtcgaaggac aacttcttct tcggcgtcag
      301 ggcggtggat aacgatggta accgcagtcc ggtgacgttc ccgcggccga tgaccagaac
      361 gcagccgcct aatcgacgta cgacgacgag cagcggcaat ccgtgacttt ctttcttcga
      421 ctaagtagct caatgaagcg tctaaggtga tatgagattt tcgaaaaaaa gactgatagc
      481 tgccatggtg attgccgcaa taacggcgtc gcagaccctc gtcgcatctg ctgacacgca
      541 gaaaagcgcg aaaaaacggc gactcacgga agagcagcag atcattcacg tcctgaaccg
      601 gctcggtttc ggggcccggc ccggcgacgt tcaacgcgtg aagtcgtttg gtcttgaaaa
      661 ctacatcaac cagcaactga accccgaaaa aattaccgac accgtcgcag aaaacaaggt
      721 caaggacatc ggcgtgctga agctgaccac cgccgaactc tacgcgaagt ttccgcagcc
      781 tgggcagttg ttgcggcagc ttcaggcgcg cggcatgttg cccgcggatt tacaaagaga
      841 gccgcaaacc aacccgccca atccgcagga cgaaagatat cgcaaggtca ttgatgagta
      901 ttaccggcag aacgggctac agcggccgca acggatcatg gcggaacttc acgcatcgcg
      961 aatcctgcgc gcggtctaca gcgaacgcca gctacaggaa gtgatggtcg acttttggac
     1021 caaccacttc aacgtctttg ccggcaaggg cgccgaccgc tggctgcttc cggcctacga
     1081 ccgggacacg atacggccca acgcgatggg caagttttca caattactgc tcgctacggc
     1141 gcaaagtccg gcgatgcttt tctatctcga caactttcag agcgtcagtc cgaatgcaaa
     1201 ccgtggctac gctcggcaac cgctgaatgc gcagcggcgg cgcggaatca acgagaacta
     1261 cgcgcgcgag ttaatggagt tgcacacgct cggcgttgat ggtggttaca ctcagaagga
     1321 cgtccaggaa gttgcccgct gttttactgg ttggaccatc tttcagcccc gcggtggcgc
     1381 cgccgcagcg aatgccatga tgggcgaagc cggacgccga aatgcgggaa ccttcttctt
     1441 caactcgcgg atgcacgacg acggcgagaa aacagtgctg ggtcacaaga tttccgccgg
     1501 tggcgggttt aaagacgggc tgacggtgat cgacatcctc gcgcgccacc cgtcgacggc
     1561 aaaatttatc gccacgaagc tcgtgcgtca cttcgtttct gacacgccgc ctgcagcgct
     1621 cgtcgaacgt gtagctgccg cttttgcgaa gagtgacggc gacattcgcg aaaccctaaa
     1681 agcaatcttc ttctcgagcg aattcaactc tgcggaagct taccgcgtaa aaattaagcg
     1741 gccctttgaa ctggcggtga gtgcgattcg agctctgggc gctgacacga atggcgggcc
     1801 gggtacgcat cagtggatcg cgcgcatggg cgagccgctt tacggatttc aaacgccgaa
     1861 tggttattcc gatgcggcag agagttgggt caacaccggc ggcttactcg agcggatgaa
     1921 tttcggtttg gcgctggccg gcaatcgaat tccgggaacg cgagtggatc tcgcgaaact
     1981 agccggacaa gagaagtcga agctgatcga cgagtcgttg aagctgatgg tggcgggcga
     2041 ggtctctgcc gcgaccagag agacgttgat gaagcagatc aacgaatcga gcgccgacac
     2101 ggcggccaag gtggtcgggc tgattctggg cacgccggag tttcaaaggc agtaagaaga
     2161 attgagtacc caggcggctt tgcctcaaaa gtcctctctc attctcaccg ggctttagcc
     2221 cggtgcagga gcagtgaaag accgaggaaa ccgtttcaac ggttttccgt tgattcgcca
     2281 gcgaaaccgt taaaacggtt tctcaaaacc gcgtcgcggt aacaccgggc tgaagcccgg
     2341 tgagaatgaa agaagacttt tgaggcaaag cctacccagg cactttaggt gtttctattg
     2401 cagggaccgc aggcgtttcg cctgcatgac tcgcgacatt cgtgtcctga cagtaaagca
     2461 ggcgagacgc ctgcggtccc aggttcaagg cgctcttatt tagaaggtgc agttatgaat
     2521 cgtagatttt ttctgaagtc aggcagtatc gcgttagcca gcgtgggcat gtcgctcgcg
     2581 gcgccggcgt ttctccagcg cgcaataatg gcccacaacg ccaccggcgg cagacgaaaa
     2641 actctcatcg ccatctttca acgcggcgct gtcgacggat tgaacatgat tgttccgttc
     2701 ggcgaatcga gttactacga tgcacggccc acaatcgcga taccgaaacc ggactccgct
     2761 aacgccgaag ccgcaatcag tctcgacggc ttcttcggtc tgcatccttc aatgagttca
     2821 ctcaagcctt tgtgggattc gaagcgcctc gcagtcgttc acgcttccgg gtcgcccgac
     2881 aacacgcgtt cgcatttcga cgcggaggat tacatggaat ccgctacgcc aggtgtgaag
     2941 tcgacgcctg acggctggtt gaatcgttac ctccaaagca agaaagacga acagcgctcg
     3001 ctctttcgcg cagtttcgat gacgcagcag atgccccgcg cgtttgaggg acgagcgccg
     3061 gtgctggcga tctctaactt gtcgaacttc gcgattcgcg ccggcaagtc ttccgcgagc
     3121 gttcaagatg gcttcgaagc gatctactca cgcgacaaaa acgaaacact ctctgcgact
     3181 ggacgcgaga ctttagaagc catcaactac ttgaaagaag ttaatcctgc tcagtacaaa
     3241 ccagagaacg gcgcgcagta tccgcggggt gcttttggca actcgctgct tcagattgcg
     3301 cagttgatca aagcaggcgt gggactggag atcgctttta ctgacatccg cggatgggat
     3361 acacacgtaa accagggcaa ctctcgcgga cagttagcaa acctgttgca gcagttcagt
     3421 tccggcctcg ctgcgctgta catggatctg ggccagcgaa tggatgacgt cgtcgtgttg
     3481 acgatgtccg agtttggccg gacggtgaga gagaacggta atcgtggtac ggaccacgga
     3541 cacgccaacg cgatgatggt gataggcaat agcgtgcgcg gcggtcaggt ttatggaaga
     3601 tggcccggac tcaagagcga tcagctttac gagggacgcg atctcgcgct gacaactgac
     3661 ttccgtgatg tgtttggcga agtcgccgct cgccaccttc gaactacgga tctgaagtca
     3721 gtgtttcccg gttactcgac tggcccggca aagtttaaag gacttttccg aaacggttga
     3781 tgacgtacaa catcaaaacc gcgagcaccg acacgattac cgcgagcgtg aacgcctcat
     3841 tatcacgtcc cgcctgcact gcatcgtaaa tcgcgacgga agcagtttgc gttttaccgg
     3901 gaatgtttcc ggcg
//
LOCUS       DASTEY010000176         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_762145, whole genome shotgun sequence.
ACCESSION   DASTEY010000176 DASTEY010000000
VERSION     DASTEY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762145"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VFO72_03140"
     CDS             complement(<1..1238)
                     /locus_tag="VFO72_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9478312.1"
                     /translation="MTLARRLRSLLLTVLCLSIFGTIANAQVKPARDPNQPIDQEYTN
                     KIREYTTAPYFNSPLTDYLPASPDVPTPKAVLGDVAGAPGKLPSAEDVYKYMRMLERA
                     RPERVKVYTIGTTEEGREMIAVAIASEQLMSKLEENRARLTKLADPRSINMDEAEADR
                     LVAASFPVYYITGAIHSTETGSPTALMELAYRLVVDNSPYVKSIRDSLVTLITPVVEV
                     DGRNRMVDVYNWHLANPNQNWPPLIYWGKYVAHDNNRDAIGLSLKLTRNVLDTYIKWK
                     PQVLHDLHESVPYLYDNTIGDGPYNAWVDPILTDEWQLIGWHNVSEMTKFGMPGVFAH
                     GNFDTWSPGYLMFIAATHNGISRLYETFGNAGADTVERTLSPSEYQRTWYRQNPPLPK
                     AKWSQRNNNNYQQTALLTTL"
     gene            1320..>2447
                     /locus_tag="VFO72_03145"
     CDS             1320..>2447
                     /locus_tag="VFO72_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9478313.1"
                     /translation="MSDQIRKAKAAGGVPSIVAAVRSLSNEMGLVRGVRTLLHVNQAG
                     GVDCPGCAWPEPDRKRSHFEFCENGAKHVADEATTKRVTPEFFQKWSVAELSEQSDQW
                     LNAQGRLTHPMLLRRDATHYEPVSWGDAFELVAAELNSLNYPDQAIFYTSGRTSNEAA
                     FLYQLFVRQFGTNNLPDCSNMCHESSGSALSETIGVGKGTVTLEDFDKAEAIFVIGQN
                     PGTNHPRMLAALEHAKRNGCKLVHINPLPEVGMTRFKHPQHVLGLLGSGTQLADLFLQ
                     VRINGDVALLKGISKAVLAREGALDRDFIDSYTDGFESFVSALQSVSWDEIVEQSGVA
                     RELIEQAAEIFVASERTIFCWAMGLTQHRNAVANIQEIVNLM"
BASE COUNT          505 a          643 c          742 g          557 t
ORIGIN      
        1 tggagcgtcg taagcaatgc cgtttgctgg tagttgttgt tgtttcgctg cgaccacttt
       61 gcctttggca acggcggatt ctggcggtac cacgttcgtt gatactcgct cggcgacagt
      121 gttcgttcga ctgtgtcggc gccggcgttg ccgaaagtct catacaaacg actaatgccg
      181 ttgtgagtcg cggcgatgaa catcagatag cctggcgacc acgtgtcgaa gttgccgtgc
      241 gcgaagacgc ccggcatgcc aaacttcgtc atctccgaaa cgttatgcca gccgatcagc
      301 tgccattcgt cggtcaggat cggatcgacc caggcgttgt atggaccatc gccgatggtg
      361 ttgtcgtaaa ggtacggtac cgattcgtgc aggtcgtgca gcacctgcgg tttccacttg
      421 atgtaggtgt cgagaacgtt gcgcgtaagt ttcaaagaca aaccgatcgc gtcgcgattg
      481 ttgtcgtgtg caacgtattt cccccagtag attaagggcg gccagttctg gttcggattc
      541 gcgaggtgcc agttgtagac gtcgaccatg cgattgcgac catcgacttc gacgaccggc
      601 gtgatcagcg ttacgagact gtcgcgaatc gacttcacgt aagggctatt gtcgaccacg
      661 agccggtaag ccaactccat caacgctgtc ggcgagccgg tttcggtcga gtgaatcgcg
      721 ccagtaatgt aatagactgg aaacgacgcg gccaccaggc ggtcggcttc cgcttcgtcc
      781 atgttgatgc tgcgcggatc agcgagcttc gtgagccggg cgcggttctc ctcgagtttc
      841 gacatcagtt gctcggaagc gatcgccacc gcgatcatct cgcggccttc ttccgttgtg
      901 ccgatcgtat agactttaac tcgctccgga cgcgcgcgct cgagcatgcg catgtacttg
      961 taaacgtcct ccgcagacgg cagcttgccg ggcgcgcccg ccacgtcacc gagcacggcc
     1021 ttaggcgtcg gcacgtcagg cgaagcgggc aggtagtccg tgagaggcga gttgaagtaa
     1081 ggcgcggtcg tatactcgcg aatcttgttg gtgtactcct gatcgatcgg ttgattcgga
     1141 tcgcgggccg gcttgacttg ggcgttggcg attgttccga agattgatag gcagagaacg
     1201 gttagaagga gcgaccgaag gcggcgcgca agcgtcatat tgttttttcc tcgtcaggct
     1261 tttaacacag attagctgga ttttgaaatc ggagatttcc gtgtaatctg ttgaaccgga
     1321 tgtcagatca aatcaggaaa gccaaggctg ctggtggcgt gccgtcgata gttgcggcgg
     1381 tcaggagtct ctcgaatgag atgggtcttg tccgtggcgt gcggacactg cttcatgtaa
     1441 atcaagcggg tggagtggat tgtccgggtt gcgcgtggcc ggagccggat cgcaagcgtt
     1501 cgcattttga gttttgtgaa aacggcgcga agcatgttgc ggacgaagcg accaccaaac
     1561 gagtcacgcc ggagttcttt cagaagtgga gcgtggctga gctttcggaa caatccgatc
     1621 aatggctgaa cgcacaggga cgactcactc atccgatgtt gctgcgacgt gacgcgactc
     1681 actacgaacc cgtgagctgg ggagatgctt ttgaactggt tgccgctgaa ttaaattcgc
     1741 tcaactatcc ggatcaggcg atcttctaca cttcgggccg caccagtaac gaagccgcgt
     1801 ttctttatca actctttgtg cgccagtttg gcacaaacaa tctgcccgat tgttcaaaca
     1861 tgtgccatga gtcgagcggc tcggcgttga gtgagacgat tggcgtcggg aagggaactg
     1921 tcacgcttga agatttcgac aaagcggagg cgatctttgt catcggtcaa aatccgggca
     1981 ccaatcaccc gcgcatgctg gctgccctcg agcacgcgaa acgcaacggc tgcaaactcg
     2041 tccacatcaa tccgctgccc gaagtgggca tgacgcgctt caaacacccg cagcacgtgc
     2101 tcggactgct cggctcgggc acacaactgg cagatctgtt tttgcaggtg cgtatcaatg
     2161 gcgacgtagc gctattgaaa ggaatctcga aagccgtgct cgcgcgtgaa ggcgcactcg
     2221 atcgcgattt catcgatagc tacactgatg gtttcgaatc gtttgtctcg gctctgcaaa
     2281 gtgtctcgtg ggacgagatc gtcgaacaga gcggcgtggc gagggaactg atcgaacaag
     2341 cggcagagat cttcgtcgca tcggagcgca cgatcttttg ttgggccatg gggctgacgc
     2401 aacaccgcaa cgccgtcgcc aacatacagg agatcgtgaa cctgatg
//
LOCUS       DASTEY010000177         4717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_531196, whole genome shotgun sequence.
ACCESSION   DASTEY010000177 DASTEY010000000
VERSION     DASTEY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531196"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFO72_03150"
     CDS             complement(<1..1187)
                     /locus_tag="VFO72_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9478314.1"
                     /translation="MLRGLFRTKKLDEILATAGGPQFSLKRTLNAFNVTLIGIGAIIG
                     AGIFATIGTAASGTVDRAGAGPSLMLSFVITAIVCGFTALCYAELASMVPISGSAYTY
                     SYATLGEIVAWIIGWDLIIEYGIGNVAVAISWAEFFNRLLSNLGIHIPEWLTSNYRTA
                     TDETLANAPQIFGIPIVFNILAFGIVALITVVLVWGIKESSNFNAIMVFIKILVLLFF
                     VGLALYYVSPATMVENWQPFQPQGWRGTFTGAALVFFAYIGFDAVSTVAEETKNPARD
                     LPIGIIASLVICTVLYIVVAGVFTGMVPWETVRDWTAAQRGESLTMALETVAPQALWQ
                     NTVVAFGSVIAHTAVLLVFQLGQPRIFFSMARDGLLPSRFASVHPRFKTPHVTTILTG
                     VAVG"
     gene            1281..2237
                     /locus_tag="VFO72_03155"
     CDS             1281..2237
                     /locus_tag="VFO72_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9478315.1"
                     /translation="MLPFRNILFPTDFSAHSHAGLKYAAGFARDGGGRVLMLNVQDAK
                     VPANLLTLPAYVFEDQENHWLLELRAKAKELLADPLLDGVEVDPLFVEGDPAKEIARV
                     AFEHEVDLITVVTRDRNRFSRAFGGSIAEEIVAESPCAVLAIRPPQRDFVEHRAGQTQ
                     IRLNRVLLATNFRPSSAAATQIATNISNHAGAELHAVYVIGDYFEQISVMFPEGGLAA
                     LTRLRSYVQERMAQFSRTFGSRVSTHVAEGRPYQEIVKLAAQLDADLIVIGTAVHGSL
                     FGNSQVLGSEIERVIRNAPCPVLCVPAAKVVTPLPALVTQAV"
     gene            complement(2380..2718)
                     /locus_tag="VFO72_03160"
     CDS             complement(2380..2718)
                     /locus_tag="VFO72_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9478316.1"
                     /translation="MRLKVLYATLVTCLAIVFTACGLIDGKTNSQAQRATNTPSVTAT
                     PYPDGARRVTIQELESLMKEGKAFVVDVRTQESYDAGHIPGAKLIPSADILNHLKELP
                     RDKIIVTYCA"
     gene            2927..4522
                     /locus_tag="VFO72_03165"
     CDS             2927..4522
                     /locus_tag="VFO72_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9478317.1"
                     /translation="MTDSPAPIQWAKVIDHTRCIGCHACTTACKSENVVPVSVTRTYV
                     KHVDIGVFPQARRAHQVTRCNQCAHAPCVTACPTSAMFKRPDGIVDFDKSICIGCKAC
                     MAACPYDAIFINPEDHSAEKCNFCAHRIDIGLEPACVVVCPTQAILVGDMNDADSYVA
                     QIVNRESVMVRRPEKETLPKLFYKGAHQATLDPLAARRPEGGLFMWSEQQANNDTVVS
                     GNSNYNNSSAAALLSYDVAHSIPWDWRVSLYTWTKGIAAGVYLVAALLALFGTMNVNE
                     SSWLWATPVISGAFLAITGGLLLWDLEHPERFFLIFARPQWRSWLVKGAFIIAAYALV
                     LMLHFVASWLGSTRFQLWLMIAGVPLSALTAVYTAYLFAQAKARDLWQNPLLPPHLLV
                     QSMLLGSAMLLLAFVGLRGWLDTDSTLDFVMTPVRSEIIDLVWLLGWTSLIHLLMIWG
                     EVSLTHPTAHARLAIHEMVRGLYRRGFWVGIGLSLLGGSLPLLAFFGVGSIAAVTGAL
                     LALMGMMLFENAYVQSGQSVPLA"
BASE COUNT          942 a         1273 c         1393 g         1109 t
ORIGIN      
        1 ccaaccgcga cgcccgtcag aatcgtcgta acgtgtggtg tcttgaacct cgggtgaacg
       61 ctcgcgaacc tcgaaggcag caatccgtca cgcgccatcg agaaaaagat gcgtggctgg
      121 ccgagttgaa acaccagcag caccgccgtg tgcgcgatca ctgatccgaa cgcaacaact
      181 gtgttttgcc acaacgcctg cggcgcgact gtttccaacg ccatcgtcag cgactcgcca
      241 cgctgcgccg ctgtccaatc gcgtaccgtc tcccacggca ccattcctgt aaacacaccc
      301 gccaccacga tgtagagcac cgtgcagatg acgagcgacg caatgattcc gatgggcaga
      361 tctctggcgg ggttcttcgt ctcctccgca acagtgctga cggcatcgaa acctatgtaa
      421 gcaaaaaaca cgagcgccgc gcctgtaaac gtgcccctcc agccctgtgg ttggaacggt
      481 tgccagtttt ccaccatggt cgccggcgag acgtaataaa gcgcgagtcc gacgaagaag
      541 agcaacacga ggatcttgat gaacaccatg atcgcgttga agttcgacga ctccttgatg
      601 ccccaaacca gcacgacagt aatcaatgcc acgataccga aggccaggat gttgaagact
      661 atcggtatgc cgaagatctg cggtgcattg gcaagggttt catcggttgc ggtgcgatag
      721 ttcgacgtga gccactcggg aatgtgtatg ccgagattgc tcagtaatct attgaaaaac
      781 tccgcccagc taatcgcgac ggcgacgttg ccaatgccgt actcaatgat caggtcccaa
      841 ccgataatcc aggcgactat ttcgccgagt gtggcgtagg aataggtgta agccgagccc
      901 gagatcggca ccattgaagc gagttcggcg tagcaaagag cggtgaaccc gcaaacaata
      961 gccgtgatga caaacgacag catcaacgat ggaccagcgc ccgcgcggtc gacggtgccc
     1021 gaagcggcgg taccgatcgt ggcgaagatt ccggcgccga tgatggctcc gattccgatc
     1081 agcgttacat tgaaggcgtt gagagttcgc ttgagtgaaa attgtgggcc accggcagtg
     1141 gctagaattt cgtcgagttt cttggtccga aagagtccgc gaagcaatgt gtcgcctcca
     1201 tggagtggat tcgcaaccgg agcctgcgga gtttcgcacg gtcttgtaaa tcttgcaatc
     1261 ctgtcttagt gttggggcat atgttgccgt tcagaaacat cctctttccc acggattttt
     1321 ctgcgcactc tcatgccggt ttgaaatacg ccgccggctt cgcgcgcgac ggcggtgggc
     1381 gcgtgttgat gttgaacgtg caggacgcga aagtgcctgc aaaccttcta acgttgccgg
     1441 cctacgtttt cgaggaccag gagaaccatt ggctgctcga gttgcgcgcc aaagcgaaag
     1501 aactgctggc cgatccgttg ctcgacggcg ttgaagtgga tccgttgttt gtcgaaggcg
     1561 accccgcaaa ggagattgcg cgcgtcgcgt tcgagcatga ggtggatctg attacggttg
     1621 ttacgcgcga tcgcaatcgt ttttcgcgcg cgtttggtgg ttctattgct gaagaaatcg
     1681 tggctgagtc gccttgcgcg gtgctggcga tacggccgcc gcagcgcgac ttcgtggaac
     1741 atcgtgccgg tcaaacgcaa attcgtctca accgcgtgct actggcaacg aatttccgcc
     1801 cttcgtcagc ggccgcgact caaatcgcta cgaacatctc gaatcacgcc ggcgcggaac
     1861 ttcacgccgt ctacgtcatc ggcgattact tcgaacagat ttccgttatg tttcctgaag
     1921 gcggactggc ggccctgacg agattgcgca gttatgtgca ggaacgcatg gcgcaattct
     1981 cgcgcacgtt tggatcaaga gtgtcaacgc acgtcgccga gggccgccct tatcaggaga
     2041 ttgttaaact ggctgcgcag ctcgacgcgg atctgatcgt gattgggacc gcggttcatg
     2101 gttcgctgtt tggaaatagt caggtacttg ggtcggagat tgagcgagtg atacgcaacg
     2161 cgccgtgtcc ggtgctttgt gtgccggcgg cgaaagttgt gacgccgctg cccgcgcttg
     2221 tgacgcaggc agtttaggcg ttaccggatc ctacttcttc ttatcgatcg gtttcccttc
     2281 cttctgccac aagttgaaac cacccagcag cgctgctgcg ttttcgattc ctttgctttt
     2341 gagagcgacc accgcaccgg cggacgtatg ttcattcggt caagcacagt acgtcacgat
     2401 gatcttgtcg cgcggcagtt ccttcaaatg attcagaatg tcggccgacg gaattagctt
     2461 cgcgccggga atgtggcccg cgtcgtatga ctcctgcgtg cgcacgtcaa cgacgaaggc
     2521 tttgccttcc ttcatcaacg actcgagttc ctggatggtg actcggcgcg cgccgtcagg
     2581 gtagggcgtt gcggtgacgc ttggtgtatt cgtggctcgt tgtgcctggc tgttggtctt
     2641 gccgtcgatc aggccgcagg ccgtgaagac tattgccaga cacgtgacca gagtcgcata
     2701 taaaacttta agacgcatag ttcctcccac ttgtgcttaa tttgtggtgc gcggcaggat
     2761 ttgtcaatcg ctgggacatt tcctgggacc gcaggcgtct cgcctgcgaa gctacggacg
     2821 ggagcctgat gtgtttcaat gcaggcggga cgcctgcggt cccaggatag agacgcctac
     2881 gctcgcagga tgctatagtc tgggctccca acgctggagc ttatctatga cggactcgcc
     2941 ggccccgatt cagtgggcta aggtaatcga ccatacgcgc tgtataggtt gtcatgcgtg
     3001 cacgactgcg tgtaagtccg aaaacgtggt tccggtttcc gtcacgcgca cgtacgtcaa
     3061 gcacgttgac attggcgtgt ttccgcaggc gcgacgcgca catcaggtga cccgctgcaa
     3121 tcagtgcgcg cacgccccgt gtgttactgc ttgtccgacg tcggcaatgt tcaaacggcc
     3181 ggatggcatt gtcgattttg ataagtcgat ctgtatcggt tgcaaggcgt gcatggcggc
     3241 gtgtccgtac gacgccatct ttatcaaccc ggaagatcac tcagcagaga agtgcaactt
     3301 ctgcgcgcat cgcatcgata tcggactcga gcctgcgtgc gtggtcgtgt gtccgacgca
     3361 ggcgattctg gtcggtgaca tgaacgatgc tgattcatac gtcgcgcaga tcgtcaatcg
     3421 cgaaagcgtg atggtgcgac gtccggaaaa ggagacgctg ccgaaacttt tttataaagg
     3481 cgcgcatcag gcgacgctcg atccgctcgc agcacggcgt cccgaaggcg ggctcttcat
     3541 gtggagcgag cagcaggcga ataacgatac ggtcgtttcc ggcaattcaa actacaacaa
     3601 ctcttcagcg gcggcgttgc tgtcgtacga cgtggcgcat tccattccgt gggattggcg
     3661 tgtgagttta tacacgtgga cgaaaggaat tgccgcgggt gtttatctcg ttgcggcgtt
     3721 gctggctttg tttggcacga tgaacgtgaa cgagtcgagc tggctatggg ccacgcccgt
     3781 tatctcgggc gcctttttgg cgatcactgg cggtttgttg ctttgggatc tggaacatcc
     3841 cgaacgcttc tttctgatct tcgcgcggcc gcaatggcgc agttggctgg tgaaaggcgc
     3901 ttttattatt gcagcgtatg cgctggtgtt gatgttgcat ttcgtggcga gttggcttgg
     3961 ttcaaccagg tttcaactgt ggttgatgat tgccggcgta ccattatcag cattaacggc
     4021 ggtttacacg gcgtatcttt tcgcgcaggc gaaggctcgt gacttgtggc agaacccgtt
     4081 actcccgcca cacttgttgg tccaatcgat gctgttgggt tcggctatgc tgttgctcgc
     4141 gttcgtcggc ctgagaggat ggttggacac ggactcgaca ctcgacttcg tgatgacgcc
     4201 tgttcggtct gagattatcg atctggtctg gcttctgggt tggaccagct tgattcacct
     4261 gctgatgatc tggggcgagg tctcgttaac gcatccgacc gcacacgccc gactcgcaat
     4321 tcacgaaatg gtccgcggac tttatcgacg cggcttctgg gtgggaatcg gcctctcact
     4381 gctcggcggt tcactgccgt tactggcatt cttcggggta ggaagtatcg ctgctgttac
     4441 gggagctttg ctggcgttaa tgggcatgat gcttttcgag aacgcttacg ttcagtctgg
     4501 gcagtccgtg ccgcttgcat agcgaacgat cccaaacgcc gcaaccatgt tagactgcaa
     4561 ccgaaccaac cctttcgagt tgaggcgttt atgaagagct acaaaacaac atcatctgag
     4621 cctttgggtt cattcgaaga aatattgcgt gcggctttgg accttcctcc gaatgccagg
     4681 gcgatgctgg ccggtcatct gcttgagagt ttagatg
//
LOCUS       DASTEY010000178         4092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1421394, whole genome shotgun sequence.
ACCESSION   DASTEY010000178 DASTEY010000000
VERSION     DASTEY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421394"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1172
                     /locus_tag="VFO72_03170"
     CDS             150..1172
                     /locus_tag="VFO72_03170"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET9478318.1"
                     /translation="MPVASYADVVSKVAPAVVTIHSRVRVRQPQQYPFMDDPFFRRFF
                     GDRNMAPPPQERQRTGLGSGVIIDPNGYIVTNHHVIDGADQIKVDLSDNRTLDAKVVG
                     TDPPSDLAVLKVEASNLPVIPLGDSDRTRVGDVVLALGNPLGVGQTVTMGIISAKGRQ
                     TGLSNGAFEDFLQTDAPINRGNSGGALVSTNSELIGINSQILSPSGGNIGLGFAVPSN
                     MARTVMEQLINSGRVRRGQLGIVVLKIPSEEAAQLGVTEGPGVVVYRVQSGSAADRAG
                     LRPGDVITALNGTAVSEPNTFRNAIASLAPGTQVTLAVRRDGNERQVLATLGEFTPPA
                     ESGRQQ"
     gene            1193..1966
                     /locus_tag="VFO72_03175"
     CDS             1193..1966
                     /locus_tag="VFO72_03175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9478319.1"
                     /translation="MKLIQQINRTLKAMSFSAKACKIILYDHGYFASIQNGELRDRDK
                     NYLPWFSYPAIEALGNWNLTDKRVFEYGSGYSTLFWASRAKEVVSVEHKGTWYAKIAR
                     LAPANVRLILAPIEKDEAEPSPELREQFNRYAGAIDGSFHIILIDGYARSRVRYQCAQ
                     MALPHLESNGLIILDNADWLPASALFLRRAGLIEVDFNGPAPGNNHTQTTSFFFTREF
                     NFEPAGSRQPRMPVGGKPDNWEKSLEAKLLAEDGSSLTL"
     gene            complement(1993..>4092)
                     /locus_tag="VFO72_03180"
     CDS             complement(1993..>4092)
                     /locus_tag="VFO72_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9478320.1"
                     /translation="VQDDQRLEIRGDKDDPFSRGYICPKAVALQDIHFDTDRLRHPVR
                     RTVSGWQRISWDQAFDEVAQNLKRINATYGRNSIATYLGNPTVHNYGALLFAPGFIRS
                     LRTRNRFSATSVDQLAHHVAGYLMFGHQLLLPVPDVDRTSFFLILGANPVVSNGSLMT
                     APGMGRRLQEMRQRGGRLVLVDPRYNETARLADRHLFIRPGTDVLLLLALLHVVFAEG
                     VVRLGQLASFTKGVDVVRSVVAEFSPESVAPITQIDAGEIRRLAREFTAAKSAVCYGR
                     IGVSTQEFGGVCQWLINVLNIVTGNFDRPGGAMFATPAFDPLSAPESIAPRGSFGRWH
                     SRVRHLPEFSGELPVAALAEEILTEGPGQIKALVTLAGNPVLSTPNGRKLDRALAGLE
                     FMAAIDFYINETTSHAQIILPPTDSLERGHYDLAFHLLAVRNTAKFSPVLFEPAPDAK
                     HDWEILRELQVRMEQNGSRSVALRNFVQRFLSPETMLALSLRFGPYGGRLNPFSNGLT
                     LRKVRQAVHGIDLGPLKSCLPERLRTPDKSIDLAPEVLVKDVERVKAKLMNGASVNSN
                     GELLLIGRRQLRSNNSWMHNTERLVKGNPAKPPCTILMNPADAAHRGLSSGQNVLVRS
                     RVGEIVLPLQVSDEIMPGVVSIPHGWGHDRNGNHLKVAQQHPGASINDLTDHLTVDAL
                     CGTAAFNGTVVTVAAHA"
BASE COUNT          947 a         1139 c         1164 g          842 t
ORIGIN      
        1 caacgtggtt agtactgtgt gcactgatcg tgagtgtggt tgttttccag agtgcctgca
       61 atcgtggcaa cggcggtggc gaatatcgat tgacgactac tccgaattcc ggcactgcca
      121 acagcacgaa caccagcaac ccagcggtca tgccggtggc gtcctatgcc gacgtcgtga
      181 gcaaagtggc gccagcagtg gtcacgattc attcgcgagt gcgcgtgcgc cagccacaac
      241 agtatccgtt catggacgat cctttctttc gccggttctt cggcgatcga aacatggcgc
      301 cgccgcctca agagcgacaa cgtaccgggt tgggttcggg agtgatcatc gacccgaatg
      361 gttacatcgt gacaaaccat cacgtgatcg acggggccga tcagatcaaa gttgacctca
      421 gcgacaatcg cacactcgat gccaaggtcg tcggcacaga cccgcccagc gatctcgcag
      481 tgctgaaagt cgaagccagc aatctaccgg taatcccgtt aggcgattca gatcggacgc
      541 gcgtcggcga cgtggtgttg gcgctcggaa acccattggg cgtcggccaa acggtgacca
      601 tgggaatcat cagcgcgaaa ggccgtcaga ccgggctgag caacggcgcc tttgaagatt
      661 tcctacaaac tgacgcgccg atcaaccgcg gcaactcagg cggcgcgctg gtcagcacca
      721 atagtgagtt gattggcatc aactcgcaaa tactttcgcc ttccggaggc aacatcggat
      781 tgggattcgc ggtgccttca aacatggcgc gcaccgtgat ggagcagttg atcaattcgg
      841 gcagagtcag acgcggccaa ctcgggatcg tcgtgctcaa gataccgtct gaagaagctg
      901 cgcaattggg tgttacggag gggccgggtg tggtcgtcta tcgagtacaa tcgggaagcg
      961 ctgccgatcg cgcgggactt cgtccgggcg acgtcatcac cgcactcaac ggtacggcgg
     1021 tcagcgaacc caacactttc cgcaacgcga ttgcgagtct tgcaccaggc acacaggtaa
     1081 cgcttgcggt gaggcgcgac ggaaacgaac ggcaagtgct ggcgacactc ggcgagttca
     1141 cgccacccgc ggaaagcgga cgccaacaat gaggtgactt tgcacaggtt ggatgaaact
     1201 gattcagcag atcaacagga cgctgaaagc gatgagcttc agcgccaaag cgtgcaagat
     1261 tatcttgtac gatcacgggt acttcgcaag catacagaat ggcgaacttc gcgaccgcga
     1321 caagaactat ctgccctggt tcagctaccc ggccattgaa gcgctcggta attggaactt
     1381 aaccgataag cgagtcttcg agtacgggtc aggatactca acactgttct gggcgtctcg
     1441 agcgaaagaa gtagttagcg tcgagcataa aggaacatgg tatgcaaaga ttgccaggct
     1501 cgcgccggcg aacgtgcggc ttatactcgc gcctatcgaa aaggacgaag ctgaaccctc
     1561 acctgaattg cgcgagcagt tcaataggta cgcgggagct atcgacggct cctttcacat
     1621 aatcctgata gatggatacg cacgatcgcg cgtccgttat caatgtgctc agatggcgct
     1681 cccgcatctc gagtcaaacg gtctcatcat tctggacaat gcggactggt taccagcaag
     1741 cgctctgttc ttacgcaggg cgggactgat cgaagtggat ttcaatggac cagcgcccgg
     1801 aaacaaccac actcaaacca catcattctt cttcacacga gaattcaatt ttgaaccggc
     1861 aggatcgcgg caacctcgaa tgccggtcgg cggcaagcca gacaactggg aaaaaagtct
     1921 tgaagcaaaa ttgcttgccg aggacggaag tagcctgacg ctgtgaaata cgtccgccga
     1981 gacgttggac gatcacgcgt gtgcagcaac tgttacgacc gttccgttga atgcggcggt
     2041 gccgcagagt gcgtcgaccg ttagatggtc tgtcaaatcg ttgatgcttg cgccggggtg
     2101 ttgttgcgcc acctttagat ggtttccatt tcgatcgtga ccccagccgt gtggaatgct
     2161 gacgacgccc ggcattatct cgtcagacac ctgcaacggc aacacgatct cgcccactct
     2221 cgaacgcacc agaacgtttt gaccggacga cagaccgcga tgagcggcgt ctgcggggtt
     2281 catgagaatc gtacatggcg gtttcgccgg attgccttta acgagtcgtt cagtgttgtg
     2341 catccacgag ttgttactgc gtagctggcg ccggccaatc aataacaact cgccgtttga
     2401 attaacagac gcaccattca ttagttttgc tttcacgcgt tcgacgtctt taacgagaac
     2461 ttccggcgcc aagtcgatgc ttttgtctgg agtacgcaag cgctcaggca aacaggactt
     2521 caatggacca agatcgattc catgaacagc ttgtctcacc ttgcgcaatg ttaatccgtt
     2581 tgaaaacggg ttcagccggc caccatatgg tccaaaacgt aagctcaaag ccagcattgt
     2641 ttctggactt aaaaatcgct ggacaaagtt tcgcaacgcc acactgcgcg aaccgttctg
     2701 ctccattcgt acttgcagtt ctcgcagaat ctcccagtcg tgtttagcgt ccggggccgg
     2761 ttcgaacagg acaggtgaaa acttcgcggt attacgaact gccagcagat gaaaagcgag
     2821 atcgtaatgt ccacgctcga gcgaatcggt tggcggtaag atgatctgcg cgtgactggt
     2881 tgtttcgtta atgtaaaagt cgatcgcggc catgaattcg aggcccgcga gcgcgcggtc
     2941 caacttacgc ccgttcggtg tcgagagcac ggggttaccc gctaaagtaa cgagcgcctt
     3001 aatctgtccc ggtccttctg tgagtatctc ctcggcgagt gcggcaacgg gcagttctcc
     3061 ggaaaactca ggcaggtgtc gcacgcggct gtgccagcga ccaaaacttc cgcgcggtgc
     3121 tatggactcc ggcgcactga gcggatcaaa cgcgggtgtg gcaaacatcg cgcctcccgg
     3181 ccgatcgaaa ttgcccgtga cgatgttcag aacgttaatc agccactggc aaacgccgcc
     3241 gaactcctgc gtgctcactc cgatccgtcc ataacagaca gcggacttcg cggcagtaaa
     3301 ctctcgcgcc agacggcgga tttcaccggc gtcgatttgt gtaatcggcg cgacgctttc
     3361 cggcgaaaac tccgcgacga cactacgcac gacgtccaca cctttagtaa acgaagcgag
     3421 ttggcccagg cgcacgacac cttccgcgaa aacaacatgc agcagtgcga gtagcaacag
     3481 aacgtctgtt cccggacgaa taaacagatg acgatccgcc agtcgggccg tttcgttgta
     3541 gcgcggatcg acgagtacta acctgccgcc gcgctgccgc atctcttgca accggcggcc
     3601 cattcccggt gccgtcatca ggctgccgtt tgagacgacc ggattcgcgc cgaggatgag
     3661 aaagaagctg gtgcgatcga cgtccggaac cggcaacagc agttgatgac caaacattaa
     3721 gtaacccgcc acgtgatgcg cgagttggtc cacggacgtt gccgaaaacc tgttacgcgt
     3781 gcgcagactg cggatgaatc ccggtgcaaa cagcagagcg ccgtagttgt gaacggttgg
     3841 attaccgagg taggtggcga tggagtttct gccgtaggtt gcgttaatgc gtttgaggtt
     3901 ttgtgctacc tcatcgaagg cctggtccca actgatgcgt tgccagccgc taacagtgcg
     3961 gcggacagga tgtctcagcc ggtccgtgtc gaaatgaata tcttgtagcg caacagcttt
     4021 ggggcaaata tatccgcggc tgaacggatc gtctttatcg ccgcgaatct ccagccgctg
     4081 gtcgtcttga ac
//
LOCUS       DASTEY010000179         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1470795, whole genome shotgun sequence.
ACCESSION   DASTEY010000179 DASTEY010000000
VERSION     DASTEY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470795"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFO72_03185"
     CDS             <1..679
                     /locus_tag="VFO72_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478321.1"
                     /translation="KDYERHRAAEAVREGSNLYVMKALFEATDFEQKFRSLKEQLDAA
                     TEVISTSAKMEADKIQFQILYAAPIEKIRVETVFQQAVLAGKSLAARLGKEITFVVGA
                     GEHLLNRLQADALADALLHLVRNAVDHGIESRGKVTLGTQTSSGTLRVFVSDNGRGIA
                     SANLPLIFEPGFSTATEVTELSGRGVGLDAVKAAVEQFGGTVTVTSEPGRGSSFEIKL
                     PNPSSDA"
     gene            complement(648..2471)
                     /locus_tag="VFO72_03190"
     CDS             complement(648..2471)
                     /locus_tag="VFO72_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9478322.1"
                     /translation="MKKKTNQRNAKKPGANVDRRNFVKALPALGIAGMAASRLPLDVL
                     AQTPAASPTPTPSPSPSPTRAPRITKEMMRQAEKLIGIELTDAQEAMALGGVNRNLNS
                     YEAIRKVDVPLDTEPAIAFHPARAKKELYVQRTRFRFGKVNPLQFKTIEDLAFATVPQ
                     LAELLRTRRVSSTELTKMYLARLKRYGPKLLCVVTLTEELALKQAAAADAELKRGKYR
                     GPLHGIPWGAKDLFATKGIKTTWGAEPYRDQMIDYDATVVERLNEAGAVLVAKLSMGA
                     LAQGARWFAGVTRNPWQPEEAQTGSSGSSAGAASATAAGLVGFSIGTETLGSIVSPSS
                     RCGCTGLRPTYGRVSRYGAMGLSWTMDKIGPICRGVEDCAAALSAIYGPDGKDITVGD
                     APFNWNADTNISTLRIGYLKNEFEGTGSQTMNEQQRRTAEQRRAVYKTALEALEKAGA
                     KLTPIELPKIPAGSLRFILTAEAAAAFDDITRDGRVNQLSGQSPGDWPNTFRTSRFIP
                     AVEYLRAQRVRTLLMQEMEKLMSQWDVFVSPAPGSASLLVSNLTGHPAVVVPCGFVND
                     LPAAIMFTGGVYDEVSPLRVALAFERATKWHTMHPKMDWAT"
     gene            complement(2477..>2892)
                     /locus_tag="VFO72_03195"
     CDS             complement(2477..>2892)
                     /locus_tag="VFO72_03195"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HET9478323.1"
                     /translation="PNNPDPDLSPEGFARAQQIARVFGGAGISAIYATQFKRTQQTVK
                     PLSDRIGVPVRLLDSNQSDELVKRIQTSLSGQTIFVAGHNNTVPAIASALSGETFPTI
                     PETEYDNLFIVTVYRFGKAKVLKLKYGKETVPMNE"
BASE COUNT          598 a          860 c          795 g          639 t
ORIGIN      
        1 gaaagactat gagcgacacc gcgcagccga agcagtgcgc gaaggctcga acctgtatgt
       61 gatgaaggcg ctgtttgagg cgacggattt cgaacagaag tttcgcagtc tcaaagaaca
      121 gttagacgca gcgaccgagg tgatctcaac ctccgcgaag atggaggcag acaagattca
      181 attccaaatt ctttacgccg ccccaattga aaaaatccgt gttgaaactg tcttccagca
      241 ggcagtgctc gccggaaaat cactggccgc caggctcgga aaggaaatta ctttcgtcgt
      301 aggagccggc gaacacttgc tgaatagact gcaggccgat gcactcgcag atgcgttact
      361 ccatcttgtt cgcaacgccg tcgaccacgg tattgagtcg cgcggaaaag tcactctcgg
      421 cactcagaca agttccggaa cactccgtgt tttcgtcagt gacaatggcc ggggtatcgc
      481 ttctgcaaac cttccgctga tttttgaacc gggcttctca accgcgacgg aagtcaccga
      541 actctccggt cgcggcgtag gtctcgatgc ggtaaaggcc gcagtcgaac agttcggagg
      601 taccgtgaca gtcaccagcg aaccgggcag gggatcttca tttgaaatca agttgcccaa
      661 tccatcttcg gatgcatagt gtgccacttc gtagcgcgct caaacgccag cgccacacgc
      721 aacggtgaaa cctcatcgta cacgccgcca gtgaacatga tcgccgcggg tagatcgttc
      781 acaaacccac acggcacgac caccgccgga tgtcccgtca ggttcgacac cagcaaactc
      841 gcgcttccgg gcgccggact gacaaacacg tcccactgcg acatcagctt ctccatctcc
      901 tgcatcagca acgttcgcac acgctgcgcg cgcaggtatt cgaccgctgg aataaacctc
      961 gaggtgcgaa acgtattggg ccaatcaccg ggcgattgcc ccgacaactg gtttacacgc
     1021 ccgtctcgcg tgatgtcgtc aaacgcggcc gcggcttccg cagtgagaat gaatcgtaat
     1081 gaacccgccg gtattttggg caattcgatg ggcgtgagct tcgcgccggc tttctctaac
     1141 gcttcgaggg ccgtcttgta aaccgcgcgc cgctgttcgg cggttcgccg ctgctgttcg
     1201 ttcatcgttt gagaaccggt gccctcaaat tcgttcttta gataaccgat tctcaacgtg
     1261 ctgatgttcg tgtccgcgtt ccagttgaac ggcgcatcac cgacggtgat gtctttgcca
     1321 tccggaccgt agatcgcact caaagccgct gcgcaatctt ccacaccgcg acagatcgga
     1381 ccgatcttgt ccatggtcca actcaagccc atcgcgccgt aacgactcac ccggccgtag
     1441 gtcggacgca agccggtgca accgcaacgc gatgacggcg agacaatcga accgagcgtt
     1501 tcagttccga tcgaaaaacc aaccaaccct gccgcggttg ccgaagccgc tccggctgac
     1561 gaaccactcg atccggtttg cgcctcttca ggttgccacg ggttacgcgt cacacccgcg
     1621 aaccatctcg cgccctgggc caacgctccc atcgacagtt tcgcgaccag caccgcgccg
     1681 gcttcgttca atctctcgac gaccgttgcg tcgtaatcaa tcatctgatc gcgataaggt
     1741 tcggcgcccc aggtggtctt gatgcctttg gtcgcaaaca gatcttttgc gccccaggga
     1801 attccatgca gcggcccccg atacttgcca cgtttcaact cagcgtcggc ggctgcggct
     1861 tgtttgagtg ccagttcctc ggtgagtgtg acgacgcaga gtaactttgg accataacgt
     1921 ttcaatcgcg ccagatacat cttcgtcaat tcggtcgaac tgacgcggcg cgtacgaagc
     1981 aattccgcaa gctgcggcac agttgcgaaa gcgagatcct cgattgtctt gaactgcagt
     2041 gggttaacct tgccaaaacg aaacctggtt cgctgaacgt agagttcttt tttcgctcgt
     2101 gccggatgaa aagcgatcgc gggttcagta tcgaggggca cgtcgacctt gcgaatcgcc
     2161 tcgtacgagt tgagatttcg atttacgccg ccgagcgcca ttgcttcctg cgcgtcggta
     2221 agctcgatgc caatgagctt ctcggcctgg cgcatcatct ctttcgtgat gcgcggcgcg
     2281 cgagttggcg acggcgatgg cgacggtgtc ggtgttggcg acgcggccgg tgtttgggcc
     2341 aacacgtcta aaggcagtcg cgatgcggcc atgcccgcga tacccagcgc cggcagcgct
     2401 ttcacaaagt ttcgcctgtc gacgttcgcg ccgggcttct tagcgttgcg ttggttagtc
     2461 ttctttttca tggtgttcac tcgttcatcg gaacagtttc tttcccatac ttgagtttga
     2521 gtactttggc tttgccaaac cggtagacgg tgacgataaa caggttgtcg tattcagttt
     2581 cggggatcgt gggaaaggtc tcaccgctca acgccgacgc aatcgcggga acagtgttgt
     2641 tgtggccggc gacaaaaatt gtctgcccgc tcagactggt ctgtattcgc ttcaccaact
     2701 cgtcggactg attcgagtcg agcaacctta ccggcacacc gatgcgatct gaaagtggtt
     2761 tcactgtctg ctgcgtgcgt ttgaactgcg tggcgtagat tgcgctgatg cctgcgcccc
     2821 cgaaaacgcg cgcgatctgc tgcgcccgcg caaaaccttc cggcgacaaa tcgggatccg
     2881 ggttgttggg tt
//
LOCUS       DASTEY010000180         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_613827, whole genome shotgun sequence.
ACCESSION   DASTEY010000180 DASTEY010000000
VERSION     DASTEY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613827"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..1433
                     /locus_tag="VFO72_03200"
     CDS             630..1433
                     /locus_tag="VFO72_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478324.1"
                     /translation="MSSLSATPAVETFALVKQFGDFVAVDHIDLQVNRGSFFGFLGPN
                     GAGKSTTIKMLTGLLAPTSGKLRVLGFDIAAQPMEVKRRIGVVPEDLNLFERLTGAEM
                     LSFTGRMYGLRNEEIAQRSKELLELMELQDDPKKLIIEYSHGMKKKLSLACSLIHRPE
                     ILFLDEPFEGVDAIASRTLKDLLSRLTTRGLTVFLTSHVLEIVERLCTDIAIIAQGKL
                     LASGSLNELRKGIRVEGDGQGPVSLEEYFIHIVGGTRSSGDEEVLQWLT"
     gene            1720..2961
                     /locus_tag="VFO72_03205"
     CDS             1720..2961
                     /locus_tag="VFO72_03205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9478325.1"
                     /translation="MDSNAGLAIQSVGILLLTLLFQFMRSSIKSASLKYWTASWASLS
                     VALLSLFVGLQFQPTQKVFYSIHFFGEYLFGLLFIAGCQSQVIGAQPTKRHVYLLVPA
                     ALIALVLPYSLRDLNDQLMIHAVIVAALFAAAYVTLRKGFKPEERSDGIRVMSAALLV
                     LVVASLHYVPVSGARRGLLGITVPASYLQYVSVFDLLFEILLGFGTVMVLMEGVRREV
                     ELANRKLLEARDQLELLVQMDPLTEALNRHAFHSLLRRPENGEKAKTSGSVAVIDIDN
                     LKPINDTFGHTVGDKAIRAVARAMRSLVRADDMLFRWGGDEFLVLMFNLPQEEASRRM
                     EKLNEILAENCSRWTSVSISVTVSCGVAGFDSLTDLGKAIEAADKAMYARRNQARGRL
                     TKAVEESLIEPVWATDQHGRH"
     gene            complement(3004..>4502)
                     /locus_tag="VFO72_03210"
     CDS             complement(3004..>4502)
                     /locus_tag="VFO72_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9478326.1"
                     /translation="VRPHNPKKSGPKDIDESTDAWDTIDWLLKNVPTNNGRAGMWGIS
                     YPGFYTSMGVIDAHPALKAASPQAPIADWFIGDDFHHNGALFLPHAFNFFATLGKPRP
                     QRTTEFPPRFVHGTPDGYRFFLDLGPLPNANRKYLKGEIAFWNDLMNHPNYDDYWQAR
                     NIRPHLRNIKPAIMTVGGWFDAEDLFGALETYKAIEAANPGARNTLVMGPWFHGGWAR
                     SDGDMLGSVSFGQKTSLAYREATELPFFNCLLKDKCDRQLPEATMFETGSNQWRTYDS
                     WPPKNVESREIYFNAVGGLTFTPPQGSDEFDEYVSDPARPVPFINNIAIGMTREYMVD
                     DQRFAATRPDVLVYQTGVLDRDVTLAGPIKATLYVSTSGTDSDFVVKLIDVYPNSAPE
                     TMGGYQMLVRGEPMRARFRNSFSKPEAMSPGKVTKVEFVLPDVNHAFLRGHRIMVQVQ
                     SSWFPLVDRNPQKFVDIYQATEADFQKATQRVYRAGANASRLTVSVLK"
BASE COUNT          973 a         1250 c         1217 g         1062 t
ORIGIN      
        1 atgggtagat tttttgattt tctacctata tcaaggcttt acctcaaaag tcttctctca
       61 gtctcaccgg gcttcagccc ggtgttaccg ccacgtggtt ttgagaaacc gttttaacgg
      121 tttcgctggc gaatccatcg aaaaccgttg aaacggtttc ctcggtcttt cactgctctt
      181 gcaccgggct taaagcccgg tgagaatgag agaggagttt tgaggcaaag ccactccgcc
      241 tcccagaatc atcgatcctg agatctgttg attggttatt cggtgacgcg aggctttacc
      301 aggcgtgagt cgcgcgtgag aacgaatact attagaaact accctggctc gagaattgaa
      361 atttgttgag tcgtgcgggc cgcccgggct ctgccgcctg aaactgaaaa accgataagg
      421 gatttccgtc ggggagtggc ccgcagatta ctcctgcctc ggatcactgt caacagaaat
      481 ttgtactggt tcagtagcag ccctgatccg ccacggacta acacggacga cactgacaga
      541 atccaactca gtccgtatag tccgtggtta tctgtcgctt aattcaactt acgcttgaac
      601 tgcaaaccga aacaacgtac aaaacctcta tgtcatcgct ttccgcgaca ccggccgttg
      661 agacttttgc gctcgtcaaa cagtttggcg attttgtggc ggtcgatcat atcgacttgc
      721 aggtgaatcg cggtagcttt ttcggttttc ttggtccaaa cggcgccggc aaatcaacca
      781 ctatcaaaat gctcaccggc ctgcttgcgc cgaccagcgg caagttgcgt gtgctgggtt
      841 tcgacatcgc tgcgcaaccg atggaagtga aacgcagaat cggtgtcgtg ccagaagacc
      901 tcaacctctt cgaacgactc accggcgccg agatgctctc gttcaccggc cgcatgtacg
      961 gactacgcaa cgaagagatt gcgcaacgct caaaagaact actcgaactg atggaacttc
     1021 aggacgatcc aaagaagctg atcatcgagt actcgcacgg catgaagaag aaactctcgc
     1081 tcgcgtgctc gctgattcat cgtcccgaaa ttctctttct cgatgaacct ttcgaaggcg
     1141 tagacgccat cgcctcgaga acgctcaaag acctgttgtc gcggctcacg acgcgcggct
     1201 tgactgtctt tctcacatca cacgttctcg agatcgtcga acgtctttgc accgacatcg
     1261 caatcatcgc gcagggcaag ttactcgctt cgggttctct gaacgaattg cgcaaaggca
     1321 tccgtgtcga gggggacggc cagggccccg tgtcgctcga ggagtacttt attcacattg
     1381 tcggcggaac gcgcagcagc ggcgacgaag aggtcttgca atggctaacc taacggtcaa
     1441 ctttttaagt ccccaagtta ttgaggcgtt tgacctggga ccgcaggcgt cgcgcctgaa
     1501 atgagcgcga agcgcgaaac gatcgatgca ggcgagacgc ctgcaatccc aggattcaag
     1561 ctacttaccg caccgattcg agaaaagtct tggccggaac acacggcctc ggctaaaata
     1621 acctccggca ggtcacctta cccgtccctt aagttctcga ccttccagcc cggaaacttt
     1681 cctgttcgtc tcgttcttac ctaaacgagg tcccagtcta tggattcgaa tgccggtttg
     1741 gctattcaga gcgtcggtat cctcctactt accctcttgt tccagttcat gcggtcgtcg
     1801 atcaagagcg cgtccctgaa atactggacc gcctcgtggg ccagcttgtc tgtcgcactt
     1861 ctcagcctgt tcgtcggtct ccagtttcaa cccactcaaa aggtctttta ctcgattcac
     1921 ttcttcggcg aatacctctt cggcttgctt tttatcgccg gctgccaaag ccaggtgatc
     1981 ggtgcgcaac caactaaacg acatgtttac ctgttagtcc cggctgcctt aatcgcattg
     2041 gtcttgccct acagcttacg cgacctcaac gaccagttga tgatccacgc cgtcatcgtg
     2101 gccgccttgt tcgcggcggc atatgtcact ctacgcaaag gtttcaaacc ggaagaacgc
     2161 agtgacggca ttcgcgtgat gtcggcggcg ctgctcgttt tagtggtggc gtctcttcat
     2221 tacgttccgg tgtctggtgc gcgcagagga ttgttgggaa tcactgttcc cgcgagttat
     2281 ttacagtacg tgtcggtttt cgacctgctg tttgagatat tgctcggctt cggcacagtg
     2341 atggttttga tggaaggcgt gcgccgcgaa gtcgaactcg ccaatcgaaa gttgctcgaa
     2401 gcgcgcgatc aactcgagtt gctggtgcaa atggatcctt tgactgaagc cctgaaccgg
     2461 cacgcgtttc attcgctgct gcgccgtccg gaaaacggtg aaaaggcgaa aacctccggc
     2521 agcgttgctg tcatcgatat cgacaacctc aaaccgatca acgacacgtt tggtcacact
     2581 gtcggcgaca aagcgattcg tgcggtcgcg cgcgcgatgc gttcgctggt ccgtgctgac
     2641 gacatgctgt ttcgctgggg tggcgatgag tttctggtgt tgatgttcaa cctgccgcaa
     2701 gaggaggcgt cgcgacgcat ggagaagttg aatgagattc tcgcagagaa ttgctcgcgt
     2761 tggaccagcg tgtcgattag cgtaactgtt tcctgcggcg tggcggggtt tgattcactt
     2821 actgatctcg gaaaagcgat cgaagccgcg gacaaagcca tgtacgcgcg tcgtaatcaa
     2881 gcgcgtggaa gattgactaa ggccgttgag gaaagcctca tcgaacccgt ttgggccaca
     2941 gatcaacacg gacgacactg acagaatcca actcagtccg tattgtccgt ggttatccgt
     3001 ggatcacttc aagacactga cagtgagacg tgacgcgttg gcgccggcgc gatagacgcg
     3061 ttgagttgcc ttctgaaaat ccgcctccgt cgcctgatag atatcgacaa acttctgcgg
     3121 gttacgatcg accagcggaa accacgagct ttgaacctgg accatgatcc ggtgtccgcg
     3181 taaaaacgcg tgattcacgt ccggcaagac aaattcaact ttcgtaactt tccccggact
     3241 catggcttca ggttttgaaa aactgtttct gaaccgcgcg cgcatcggct cgccacgcac
     3301 gagcatctgg tatccgccca tcgtctccgg cgcgctattc ggatagacgt cgatcagctt
     3361 caccacaaaa tcagagtcgg ttccggaagt cgagacgtag agcgtcgctt tgattggacc
     3421 agccagcgtc acgtcgcggt caagtacgcc ggtctggtaa acaagaacgt ccggtcgcgt
     3481 tgcggcgaag cgctgatcgt caaccatgta ctcgcgcgtc atcccgatcg cgatgttatt
     3541 gatgaacgga accgggcgcg ccggatcgct gacgtactcg tcaaactcat cactgccctg
     3601 tggtggcgtg aaagtcagac cgccgacagc gttgaaatag atctcccgcg attcgacgtt
     3661 ctttggcggc caactgtcgt atgtccgcca ctggttcgat cctgtttcga acatcgttgc
     3721 ttccggcaat tgccggtcgc acttgtcttt caacagacag ttgaagaacg gcagttccgt
     3781 agcctctcga tatgcgagcg aagtcttttg accaaacgaa acactgccga gcatgtcgcc
     3841 gtcgctgcgc gcccagccgc cgtgaaacca cggccccatc accagcgtgt tgcgcgcgcc
     3901 aggattcgcc gcttcgatcg ctttgtaagt ttcgagagcg ccgaagagat cttccgcgtc
     3961 aaaccagccg ccgacggtca ttatcgcggg tttgatattg cgcaggtgtg gacggatgtt
     4021 tcgcgcctgc cagtagtcgt cgtagtttgg gtggttcatg agatcgttcc agaaggcgat
     4081 ctcacctttc aaatacttgc ggttcgcatt cggcagtggg ccaagatcaa gaaagaaacg
     4141 atagccgtcg ggagttccgt gaacgaagcg cggcggaaac tctgtagttc gttgcggacg
     4201 aggtttgcct aaggtcgcaa agaagttgaa cgcgtgcggc agaaaaagcg cgccgttgtg
     4261 atgaaaatcg tcaccgatga accagtcggc aatcggcgcc tggggcgacg ccgccttcaa
     4321 agcgggatga gcgtcaatca cccccatcga cgtatagaag ccgggatagg aaattcccca
     4381 catcccggcg cgcccgttgt tggtgggcac gttcttcagg agccagtcga tcgtgtccca
     4441 cgcgtcggtg ctttcatcga tgtcttttgg tccggatttc ttcgggttat gagggcgcac
     4501 ct
//
LOCUS       DASTEY010000181         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1635834, whole genome shotgun sequence.
ACCESSION   DASTEY010000181 DASTEY010000000
VERSION     DASTEY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635834"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFO72_03215"
     CDS             complement(<1..777)
                     /locus_tag="VFO72_03215"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HET9478327.1"
                     /translation="MKRDFVKEFLTYIQVEKGLAQHSVASYERDLTRLQTWAIQNGKD
                     LLELTRVDLRKWIASLSREGLAPTSIARAVSATRGFYKFLMLDGHIKRHPAEDLDTPQ
                     RFSYLPKFLTEEEINRLLAAPDITTLEGIRDRTVLEVMYATGLRVSEMVNLKQADVDL
                     LAGLVVCHGKGSKERKVPLGKSAIHWLQQYAAAKADYGKQTLPHVFLHRGKPFTRHLA
                     WSMIKRHAERAGIKNVSPHTLRHSFATHLLQHGADSRSVQA"
     gene            complement(774..2045)
                     /locus_tag="VFO72_03220"
     CDS             complement(774..2045)
                     /locus_tag="VFO72_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478328.1"
                     /translation="MKIRFPKGILRETLKMALDSVRGHKFRSFLTVLGIVIGVMTAIC
                     IASLLTGLRQNIVAMVEEYGTNNIYAFHLSTGPRTGEDRSERLRKPLTVADAQTIKAQ
                     APAVDDVAWVAPNVGYGGGQFDDNITYQGRNYRWANTQGVSANYSDVYNVVLKEGRFI
                     TELDDQQRSNVMVIGVNAAEALFPGSDQIAGAQVRMGGYNFEIIGVLEKRKAGFFGEN
                     EEDNAVYIPFRTAQKIAPAKGYLLLVIRGHSGQVQEALIQSEEILRRRRNVKFGDPNN
                     FDIKTADKFIEQFDSITAMVGLIAIAISSLGLLVGGIGVMNIMLVSVTERTKEIGVRK
                     AIGARRRDIVRQFLFEAMMLTFLGGVLGVLLAAGASQLLMLLIPSLPASIPTWAVVTG
                     LTVSIGVGLIFGVWPARKASRLDPIECLRYE"
     gene            complement(2058..3302)
                     /locus_tag="VFO72_03225"
     CDS             complement(2058..3302)
                     /locus_tag="VFO72_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478329.1"
                     /translation="MKAILSFYSDALKIALQSIFAHKLRAFLTLIGIIIGVASVVTVG
                     ASISGLNTYVVEKIQKLLGSNHFMIARMAFSGHMDDEEFERRNRRNKRITWDDYEWVR
                     DHCLSCAEVGAAAGNGTDLRQDGIEFPGAVVFGATANMVDIEDKIIESGRFITQDEVQ
                     RSAMVVVLGGDIKDKFFPHTDPIGKTLKIRGLPMRVVGVEEKRGAFFGDSIDRHVYIP
                     ITTHLQIFGRNGLQIHGKSHTREGFQETIEDARVAMRIKRRLNGNEEDDFGVVNVEEL
                     NNQIDEFTNSIAMVVVPITAITLLVGGIVVMNIMLVSVTERTFEVGLRKAVGATRRQI
                     LLQFLIESAILCALGGIIGLLLSWGITTLITTLASITMTITIGYILLALTVSTITGML
                     AGIYPAFKAARLDPILALTHAT"
     gene            complement(3517..>4483)
                     /locus_tag="VFO72_03230"
     CDS             complement(3517..>4483)
                     /locus_tag="VFO72_03230"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HET9478330.1"
                     /translation="LDLEHRQKGLLWYSTYKVRFTGVYGFRNTSDKDQNVIFKLQFPT
                     TQAIYDNLTFVVDGNPAALTNENNAAKTAVKIAAGKTAQLTIGYSSQGLNEWRYSFGR
                     TEVSQVKDFDLRMSTNFKDIDFPENTLSPSEKHQTGKGWDLTWSYTSLLSGYQIAMVM
                     PEKLQPGPLAGRISYFAPVSLFFFFFLMLIITTMRGIDLHPMNYFFLAAAFFSFHLLL
                     AYLVDHISIHAAFAISAAVSVFLVISYLRLVVGMRFASREAALAQFIYLVMFSYAFFL
                     KGFTGLAITIGSVVTLFVVMQVTGRVRWAEKFAVKPASEPAAEMR"
BASE COUNT          993 a         1247 c         1232 g         1011 t
ORIGIN      
        1 tgcctgaacg gagcgcgagt cagccccgtg ttgcagcaga tgagtcgcaa agctgtgacg
       61 taacgtgtgt ggtgaaacgt tctttatccc cgctcgttcc gcatgacgtt tgatcatcga
      121 ccacgcgaga tgccgcgtga acggtttacc gcgatgaaga aaaacgtgag ggagcgtttg
      181 ctttccgtag tcagccttgg cggcggcgta ttgttgcaac caatggatcg cgctcttgcc
      241 tagcggcact ttccgttcct tgcttccctt gccgtgacag accaccagtc ccgcaagcag
      301 atcgacgtcg gcctgtttca gattcaccat ttcagaaacg cgcagtccgg tcgcatacat
      361 cacttccaac actgttcgat cgcggatgcc ttccagcgtc gtaatgtcag gcgctgcgag
      421 caggcgattg atctcttctt cggtgagaaa cttcggcagg taactaaacc gctgtggtgt
      481 gtccaggtcc tccgccggat gtcgcttgat gtggccgtcg agcatgagga acttgtagaa
      541 gccgcgcgtt gcactcaccg cccgggcgat cgatgttggt gcgagtccct cacgcgaaag
      601 actcgcgatc cacttgcgca agtcgacgcg tgttagttcc agcaggtcct ttccgttctg
      661 aatggcccag gtttgcaggc gtgtgagatc tctttcgtaa ctggcgacgc tgtgttgcgc
      721 cagtcccttt tcgacctgga tgtaagtcag gaattccttt acgaagtcac gcttcattcg
      781 taacgcagac actcgatcgg atcgagtcgc gatgctttgc gtgccggcca gacaccgaag
      841 atcaatccca cgccgattga caccgttagg cccgtaacga ctgcccacgt aggaatcgag
      901 gccggcaaac tcggaatcag cagcatcaac agttggctcg cgccagccgc cagcaacacg
      961 ccaagcacgc cacccaaaaa cgtcaacatc atcgcctcaa acagaaactg gcgcacgatg
     1021 tctctgcgcc gcgcgccgat ggccttgcgc acgccgatct ctttggtacg ttcagtgacg
     1081 ctcacgagca tgatgttcat cacgccaatg ccgcccacca gcaatcccaa actcgagatg
     1141 gcgatggcaa tcagtccgac catcgcagtg atgctatcaa actgttcgat aaatttgtcg
     1201 gctgttttga tgtcgaagtt atttgggtcg ccaaatttca cgttgcggcg gcggcgcaaa
     1261 atttcttcgg attggatcag agcttcctgg acttgtcctg agtgaccgcg aattacgagc
     1321 aacagataac cctttgcggg agcgatcttt tgcgctgttc gaaacgggat gtaaacggca
     1381 ttgtcctctt cgttctcgcc gaaaaaccct gcttttcgtt tttccagaac gcctatgatc
     1441 tcgaaattat agccgcccat tcgcacctgc gcgccggcaa tttgatccga cccgggaaac
     1501 agcgcctcag cagcgttaac accgatgacc ataacgttcg aacgctgctg atcgtccaac
     1561 tccgtgatga aacggccttc tttcaagacg acgttgtaga cgtccgagta gtttgcagaa
     1621 acaccttgag tgttggccca gcggtaattg cgcccctggt aagtgatgtt gtcgtcaaac
     1681 tgtccaccgc cgtaacctac gttcggcgcg acccaggcaa cgtcgtcaac tgcgggcgcc
     1741 tgcgctttga tcgtctgtgc atcggcaact gtcagcggtt tgcgcaggcg ttccgaccga
     1801 tcttcaccgg tgcgcgggcc agtcgagaga tgaaaggcgt agatgttatt cgtgccatat
     1861 tcctcgacca tcgcgacgat gttttggcgt agcccggtga gcagcgacgc gatgcagatc
     1921 gccgtcatca cgccgataac gattccgagc acggtgagaa agctgcggaa cttatgcccg
     1981 cggacgctat caagcgccat cttcaacgtt tcgcgaagaa taccttttgg gaatcgaatc
     2041 ttcattactg caaaactcta cgtcgcgtgc gtcagtgcga gaatcggatc gagtctcgct
     2101 gctttgaatg ccggataaat gccggcgagc atgccggtga ttgtggaaac ggtcagcgcc
     2161 agcagaatgt agccaatcgt gatcgtcatc gtgatggacg ccagcgttgt gatcaaggtc
     2221 gtgatgcccc acgacaacag caacccgatg attcccccga gcgcgcaaag aatcgccgat
     2281 tcgatcagga actgaagcag gatctggcgg cgcgtagcgc ccacggcttt tcgcagtcca
     2341 acctcgaacg tcctttccgt aacactcacc agcatgatgt tcattacgac gatgccgcca
     2401 acgagcaacg tgatcgcagt gatcggaaca accaccatcg cgatgctgtt agtgaactcg
     2461 tcgatctgat tattcagctc ctcaacgttg acgactccga aatcgtcttc ttcgttgccg
     2521 ttgagccggc gtttgatgcg catcgcgaca cgtgcgtctt cgatcgtttc ctggaaacct
     2581 tcgcgcgtgt ggctcttgcc gtggatctgc aacccgttgc ggccgaagat ctggaggtga
     2641 gtggttatcg gaatgtagac gtgccggtcg atcgaatccc cgaagaaagc accgcgtttc
     2701 tcttcaacac caacgactcg cattggcagt ccgcgaattt tcaaagtctt tccaatcggg
     2761 tccgtatgcg ggaaaaactt gtccttaata tctccgccca gcacgacgac catcgccgac
     2821 cgttgcactt catcctgggt gatgaagcgt ccggattcga taatcttgtc ttcgatatcg
     2881 accatgtttg cggtcgcgcc gaacaccacc gcgcccggaa actcgatgcc gtcctgccgt
     2941 aagtcggtgc cgttgcccgc cgcggcgccg acttccgcgc aggaaagaca atggtcacgc
     3001 acccactcgt agtcgtccca ggtaattcgc ttgttgcgcc ggttgcgacg ctcaaactct
     3061 tcatcgtcca tgtgcccgga aaaggccatg cgggcaatca tgaagtggtt actgccgagt
     3121 aacttttgta tcttttcgac gacgtaggtg ttgagcccgc tgatcgatgc gccgacggtc
     3181 acaacggacg cgacaccgat gatgatgccg atcagcgtga gaaatgcccg caatttgtgc
     3241 gcaaagatcg actgtaaggc aatcttcagg gcatcggagt aaaaagacag aattgctttc
     3301 ataatcttgg cttgccgccg gtcagtacgc gtggctatac gcgaccccaa cccgcgaagt
     3361 tcaacgccac ggcaagctgg atctcacgaa gccgcaaagc cgcaaaggaa atcaggagct
     3421 tgtttcacct ttgcgccttg gcgtcttggc gcgagatttc tactcaccgt ccctaagacg
     3481 ctcagtgtcg ggcgtttatt aacagtggat atgcggtcag cgcatctcgg ctgccggctc
     3541 gctcgcgggt ttgactgcaa acttttccgc ccagcggacg cgcccggtca cctgcatcac
     3601 cacaaaaagc gtcaccaccg agccgatagt tatggcgaga ccggtgaaac ctttcaggaa
     3661 aaatgcgtag gagaacatga ccaggtaaat gaactgcgcc agagctgcct cgcggctggc
     3721 gaaacgcatc ccgaccacca gtcgcaaata actgatgacc agaaacaccg aaaccgcggc
     3781 ggatatagca aacgcagcgt ggattgagat gtgatcgacc agataagcga gcaagagatg
     3841 gaaagaaaag aacgcggcag cgaggaaaaa gtagttcatg gggtgaagat cgatgccgcg
     3901 cattgtggtg atgatgagca tgaggaaaaa gaagaagaag agtgacactg gggcaaaata
     3961 actgatgcgg cccgcgagtg gtccaggctg gagtttttcg ggcatcacca ttgcgatctg
     4021 ataacccgaa agcaggctcg tgtacgacca ggtcaggtcc cagcctttgc cggtttgatg
     4081 tttttcggac ggcgagagcg tattttcagg gaagtcgatg tctttgaagt ttgtgctcat
     4141 gcgcaggtcg aagtctttga cctgtgagac ttcggtgcgg ccgaagctgt aacgccactc
     4201 attcaatccc tgcgagctgt agccgatagt caactgcgcc gttttgccgg ctgcaatttt
     4261 gacagcggtt ttcgcggcgt tgttctcgtt ggtgagtgca gccgggtttc catcaacgac
     4321 aaaggtcagg ttgtcgtaga tcgcctgagt agtggggaat tgcagtttga agatgacgtt
     4381 ctggtccttg tcgctggtgt tgcgaaatcc gtagacgccg gtgaaacgaa ctttgtaggt
     4441 gctgtaccag agcaagcctt tctgccggtg ctcgaggtcg aga
//
LOCUS       DASTEY010000182         8344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1042230, whole genome shotgun sequence.
ACCESSION   DASTEY010000182 DASTEY010000000
VERSION     DASTEY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042230"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VFO72_03235"
     CDS             complement(<1..1353)
                     /locus_tag="VFO72_03235"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9478331.1"
                     /translation="MSEDRNDLRVQSSALSDRGLNERRPLNEDAYLQDRDRGIFAVAD
                     GVGGAEAGEVASQTAIEVLDEAFRHHTDGADVEDLMELAIQRANASIHQMAQDHARFS
                     MMATTIVALHLKGNVATFGHVGDSRLYRLTPDGQLHRETEDHSIVEEEVRAGRMTPEQ
                     AANHPSKNVISRALGAEQAVEVDMKTMEIEDGTEFLLCTDGITRHVSDNEIRQLLVLN
                     NDLDEVCRELKRRCYERGAEDNLTVVVVRVGKHIAVDERLAELEPTISPETQVVYAAQ
                     NGGQQTGAGTQSNGSYVSGSRIAIPATAPQPETHDSAVNVAEPRRSGSGLARVLGLLF
                     VLVLLAGAFYAGARYKERIPFLASNAQPQTEVTAPVTAPSPEEPFVQFERMRRQVDQD
                     ARAWLATEPQKELLNAGVQSPLDSPHTDFLYLYGRANWLSGNTDEAGKAFDAAIAKAD
                     A"
     gene            complement(1350..3041)
                     /locus_tag="VFO72_03240"
     CDS             complement(1350..3041)
                     /locus_tag="VFO72_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET9478332.1"
                     /translation="MKNRASSHLLLILLILGLEIAGYIAIHRAGLLRGYETSVIGAVR
                     DLLMFLPLMVLAIWLSRSKRFAGNWVLFTTAVLLFAFGMLIQYRLYSDPEYNARNKAA
                     AREQKMQALRTRYIMENYDPVKKQLMGLPPTPAQPIDIDQLPKKESTYSLWNAITSSY
                     TWIPILSFFAFAIAYSLCSRDGFLLWLQRNSFIIVLFTLVPLAIAVVTSSAGKALGNM
                     TPWEPSKIPFLVGFAGILTARYKDLAETYWGVPRARDIVPLLVMAILPFVPFFALKDF
                     GQMLIFSGAYTTLYLVAVRRWPQLLLFVGSLLLVIGILVVGALPRDIQQQVPLLPTIA
                     RPISAALPARIQQRFHLWLDGFDPPSPEVSWWKNEYQEALDQNPRLRELAEQSPAMQR
                     TVNVDVWFDKLAFQPAQATWGISSGGTSGRGFGLGFPEVIPIADSDYVYAAIAEETGL
                     LGGAVIIMAVIIFVIAGVRTSLEARDMFTKLCAAGLTAFIGFQAFVNIGGILRALPMT
                     GITLPFVSHGGFSLITSFAMLGMLMAFSNRNARDRIIAEIPTVKSSSPLKLEAVE"
     gene            complement(3072..4958)
                     /locus_tag="VFO72_03245"
     CDS             complement(3072..4958)
                     /locus_tag="VFO72_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9478333.1"
                     /translation="MTTLLLLLFQSPTPPVVDPHVPERTPALFTLIYVGGFAVIILFL
                     LIGLIRNRRATGPAPAAIPEDLPKEVKKRLGSHSTNRGLRALRWLFVLLSIGLFSVHV
                     YWARYAAEKNQKFQELSYKDLRLRRLSESTLRGWVLDRSGRLDQPLAYYKRDSTGDIR
                     REYPMDKAMAHLFGSDRGDPGLERALFGLQSGALPEAFAVVRGETLDFRGNYDVRLTI
                     DRSLQQAAMDQLKGKHGAVVIINPQNGEVLALYSEPSYSLKEVTDEATWIRLEANERD
                     RPLVSRALGAYYIPGSTFKTVTMIAAFLAGEQETEFTCSGSGYYAAPGANVIFDDAGP
                     SEVHGRIRIDHAYEVSCNQYFAQMGVKLGPERLKRAAQLLGIGSYDTPSEALRGRKAP
                     ELWDASSEAVKRALAPREATIVTGKQVTKYDLALMGYGQGYAGQMTPLQMALSAAVIA
                     NVEGKLMKPKIEFNVAPQPYNQVLTPAAAARMRSIMGLVTGGPSGTARGVFAPVKAAG
                     INTGGKTGTAQKVVPVYDPKTGEPKTRHRVERDNRGNIIREYDEIIMDEENPRIDGWF
                     LCIAPLERPTLAMAVIVEGGGYGSRSAAPIAAALVLKARDLGYFSGSRPATPPSLISK
                     GVNR"
     gene            complement(5052..5906)
                     /locus_tag="VFO72_03250"
     CDS             complement(5052..5906)
                     /locus_tag="VFO72_03250"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9478334.1"
                     /translation="MINSDDPTEKPKEDPAVKKRREEALPERILRRVLEGMGDVVDRR
                     FGRTVEPRTGLTTSKLIERMKKLIDERVRDEGAKGRIAPHHLILKVEWGTHSEAPPEI
                     LHELKHEVLAAAIDHINDHRYRTLGPVSVEAEVDIFTTGISVDPTFGEFEEDLRRQDE
                     ERRAAKSGVAIPSAAPPMPDIEVVARITQQNGTREVPLVFKPGGKRLNVGRASDNELT
                     LNDASVSKIHAALLMTSEGTLLVADTGSTNGTYINGRRIAYGESRAIEDGDVIGFGDV
                     EVRLRKSD"
     gene            6325..7278
                     /locus_tag="VFO72_03255"
     CDS             6325..7278
                     /locus_tag="VFO72_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9478335.1"
                     /translation="MNRKRFHPFAVVWGAALVLAVMPVALGQFTNNQQTSTAQNTALR
                     SVPAGAKMKFRGVVIGRDADVFTIRDRSRADYQVLITDNTSIKTHGGFFRSGKKYPVT
                     DILRGLIVEVEGRGDNQGQLVADKIRFSESDMRAAMTTDARVGPVEANMERVSGQMDE
                     LYAVAAEARAEVKAVNERVSALDDYDVQETVSVTFRTNSAVLSPEAKAQLDALAAKTA
                     GAKAFMIEVAGHTDSTGSDAKNFRLSRQRAEAVVQYLAVNHRIPLRRFVTPMGYGKTD
                     AVADNTTREGRSQNRRVDVKMIVNRGLNQTGTTPTSSSARP"
     gene            7760..>8344
                     /locus_tag="VFO72_03260"
     CDS             7760..>8344
                     /locus_tag="VFO72_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9478336.1"
                     /translation="MTNSEVFPILHGGTTAAKKTEPAPNSDLALAMAAGKGDMEAFEK
                     LYERHSRRVYSLCLRMTQNGAEAEDLAQEAFIQLFRKIGSFRGDSAFTTWLHRLVVNQ
                     CLMHFRKRSVKLEKTTEEGETPVQIVRGTENPNAMPVMDRIALDNALRQLPPGYRTVF
                     VLHDVEGHEHEEIARMLGVAVGTSKSQLHKARMKL"
BASE COUNT         1813 a         2400 c         2330 g         1801 t
ORIGIN      
        1 cgcgtcggct ttggcgatgg cagcgtcaaa cgcctttccg gcttcgtcgg tatttcccga
       61 aagccagttc gcgcgaccgt agaggtacaa aaagtcggtg tgcggcgagt ctaacggact
      121 ctgaactccg gcgtttaaca actccttttg aggctcagtc gctagccacg cacgcgcatc
      181 ctggtccacc tgacggcgca ttcgctcgaa ctggacgaac ggttcttcag gcgatggagc
      241 agtgacgggc gcagtcactt cagtctgcgg ctgcgcgttt gaggcgagga acggaattcg
      301 ttccttgtat cgcgcgccgg cgtagaacgc tcccgccagc aacaccaaca caaacaaaag
      361 cccgagaacg cgcgcgagac cactgccact tcttcgcggc tccgcgacat tgaccgcgct
      421 atcatgggtt tcgggttgcg gtgcagtggc ggggatcgct atgcgcgatc cggaaacgta
      481 acttccgttt gactgtgtgc cggcgccagt ttgctgtcca ccgttctgag ctgcatacac
      541 gacctgcgtc tcaggcgaaa ttgtcggctc gagttccgcc aaccgttcgt caacagcaat
      601 gtgtttgccc acgcgcacga ctaccaccgt gagattgtct tcagcgcccc gctcgtaaca
      661 acgccgcttc agttctcgac aaacctcgtc gagatcgttg ttgagtacga gtaactgccg
      721 gatctcattg tctgagacgt gccgagtgat accgtcagtg cacaaaagaa actcagtacc
      781 gtcttcaatc tccatcgtct tcatgtcgac ttcgacggcc tgttcggcgc cgagtgcacg
      841 actgatcacg ttcttactcg gatggttggc ggcctgttcc ggcgtcatgc gcccggcacg
      901 tacttcttct tcgacgatcg agtggtcttc agtttcgcga tgcaactgcc catcgggcgt
      961 gagccgatag agacgcgaat cgccgacgtg tccaaacgtg gcgacgtttc ccttcaggtg
     1021 cagcgcgacg atggtcgtgg ccatcatcga aaacctcgca tggtcctggg ccatctgatg
     1081 aattgacgcg ttggcccgct ggatcgcgag ttccatcaga tcttcgacgt cagccccgtc
     1141 agtgtggtga cgaaaagctt cgtcgagaac ttcgatcgca gtttgtgagg caacttcgcc
     1201 cgcctcagcg ccgccgactc cgtctgcgac agcgaagatt ccgcgatcgc gatcctgcaa
     1261 gtaggcatct tcattaaggg gacggcgctc attgagcccg cggtcactaa gcgctgagga
     1321 ctggacccgg aggtcgttcc tgtcttcgct cattccactg cctccagctt aaggggagat
     1381 gaactcttta ccgtcgggat ttccgcaatt atccggtcac gcgcgtttcg gtttgaaaag
     1441 gccatgagca tacccaacat cgcaaagctc gtgatcaaac taaaaccgcc gtggctcacg
     1501 aagggaagcg tgatgcccgt catcggcaag gcgcgcaaaa taccaccaat gttcacgaaa
     1561 gcttgaaaac cgataaatgc cgtcagacca gccgcgcaca acttcgtaaa catatcacgc
     1621 gcctccagcg aagtgcgaac accagcgatg acgaaaatga tgaccgccat gatgatcact
     1681 gctccgccga ggagacctgt ctcctccgca atcgcggcgt aaacgtaatc ggagtcggca
     1741 attggaatca cttccgggaa acccaacccg aaacctctgc ccgatgttcc gcccgaactg
     1801 atcccccacg tcgcctgcgc cggttggaat gcgagtttgt cgaaccagac gtcgacgttg
     1861 acagttcgtt gcatggcggg actctgttcg gcaagttctc tcagtctcgg attctgatct
     1921 aaagcttcct ggtattcgtt cttccaccac gagacctccg gcgatggcgg gtcaaaacca
     1981 tcgagccaga gatgaaaacg ctgctgaata cgcgcgggaa gcgcagcgct gatcgggcgc
     2041 gcgattgtcg gcagcaacgg cacctgctgc tgaatgtcgc gcggcaaagc gcccaccaca
     2101 agaatgccga tcaccaacag taacgaaccg acgaacagca gcaactgcgg ccagcgacga
     2161 acggcaacga gataaagcgt ggtgtaagcg ccgctgaaga tcagcatctg gccgaagtct
     2221 ttcaaagcga agaagggaac gaacggcagt atcgccatca ccagcaacgg cacgatgtcg
     2281 cgtgcgcgtg gaacacccca ataggtctca gccagatctt tgtagcgcgc agtaagaatg
     2341 cccgcaaagc cgacgaggaa agggattttc gaaggctccc acggcgtcat gttccctagc
     2401 gcttttccgg cactggaggt gacgaccgcg atcgccagcg gcaccaacgt aaacaggacg
     2461 atgataaagc tgttgcgctg gagccataac agaaacccgt cgcgactgca caacgagtac
     2521 gcgatggcga aggcgaaaaa tgagaggatg ggaatccagg tgtaacttga cgtgatcgca
     2581 ttccaaagcg agtaggtcga ttccttcttc ggtaattgat cgatatcgat cggctgtgcg
     2641 ggcgtcggcg gcagtcccat caactgcttc ttgactggat cgtagttctc catgatgtag
     2701 cgcgttctca acgcctgcat cttttgttcg cgcgcggctg ccttgttgcg cgcgttgtac
     2761 tccggatcgc tatagagccg gtactggatc agcatgccaa acgcaaacag caaaaccgca
     2821 gtcgtaaaga gcacccagtt gccggcgaac cgcttggatc gcgacaacca gattgccagc
     2881 accatcaacg gcagaaacat caacaagtcg cgcaccgcgc cgataaccga cgtctcgtaa
     2941 ccgcgcaaca acccggcgcg atgaatcgcg atgtagcctg cgatctcgag accgagtatc
     3001 agcagtatga gcaacagatg tgaagaggcg cgattcttca ttgccgattg ccgattgcca
     3061 attgccgatt gctaacggtt tactcctttc gaaatcagac tgggcggcgt cgccggacgg
     3121 ctgccgctga aataaccgag atctcttgct tttaaaacga gtgcggccgc gatcggcgcc
     3181 gcggaacgag aaccatagcc gccgccttcg acgataacgg ccatcgccag cgtaggtcgc
     3241 tcgagtggcg cgatgcacag gaaccagccg tcgatgcgcg ggttctcttc gtccatgatt
     3301 atttcgtcgt attcgcggat gatgttgccg cggttgtcgc gttcgactct gtggcgcgtt
     3361 tttggttcgc cggttttagg atcgtacacc ggcaccacct tttgcgcggt gcctgtcttg
     3421 ccaccggtgt tgatgcccgc agctttgact ggcgcaaaca cgccgcgtgc ggtgccgctc
     3481 gggccgccgg tgacgagtcc catgatcgaa cgcattcggg ccgcggcggc cggcgtcaac
     3541 acctggttgt aaggctgcgg cgccacatta aattcgatct tcggcttcat caacttgcct
     3601 tccacgtttg caatcacagc cgccgagagc gccatttgga gaggtgtcat ctgtccggcg
     3661 taaccctggc cataacccat taaggcgaga tcgtattttg ttacttgttt gccggtaacg
     3721 atcgttgctt ctcgcggcgc gagcgcgcgc ttcactgctt cgctgctggc gtcccagagt
     3781 tccggcgcct tgcgcccgcg cagagcttca gaaggcgtgt cgtaactgcc gatacccaac
     3841 aactgcgctg cacgtttgag ccgttccgga ccaagcttca cgcccatctg cgcgaagtat
     3901 tgattgcagg aaacttcgta cgcgtgatcg atacggatcc ggccgtgcac ctcgcttggt
     3961 ccagcatcgt cgaaaatgac gttcgcgcct ggcgctgcgt agtaaccact gccgctgcag
     4021 gtgaactcag tttcctgctc gccggcgaga aacgcggcga tcatggtgac ggttttgaaa
     4081 gtcgaaccgg gaatgtaata ggcgccgaga gcgcggctga cgaggggcct gtcgcgctcg
     4141 tttgcttcga gacgaatcca ggttgcttcg tcggtgactt ctttcaggct gtacgacggt
     4201 tcgctgtaga gcgcgagcac ttcaccattc tgtgggttaa taatcacgac tgccccatgt
     4261 ttgcccttca actggtccat cgccgcctgt tgcagcgaac gatcgatcgt gagacgtacg
     4321 tcgtaattac ctctgaaatc gagtgtctct ccgcgcacga cggcgaacgc ttccggcagt
     4381 gcgccacttt gaagcccaaa cagcgcgcgt tccaatccgg ggtcaccgcg atccgagccg
     4441 aacaggtggg ccatcgcttt gtccatcgga tactcgcggc ggatgtcgcc cgtcgaatcc
     4501 cgtttgtaat acgcaagcgg ctgatcgaga cggccgctgc gatcgagaac ccagccgcgc
     4561 agagttgatt cagacaagcg acgcaagcgc agatctttgt aactcaactc ctgaaacttc
     4621 tggttttttt cggcagcgta tctggcccaa tagacgtgga ctgaaaacag gccgatcgag
     4681 agcagcacaa acaaccagcg cagcgcgcgc aggccgcggt tcgttgaatg cgacccgagt
     4741 cgtttcttta cttccttcgg aagatcttcg ggaatggctg ccggcgccgg gccggtggcg
     4801 cggcgatttc gaatcaggcc gatgagcaga aacagtatga tgaccgcaaa gccgccgacg
     4861 taaatcagcg tgaacagcgc gggtgttctt tccggcacat gaggatcaac aacaggcggc
     4921 gtgggagatt gaaacaggag taagagtaat gtcgtcatcc agagttctta atacttaact
     4981 aactttcctc tctgtgcttc tgtgtctcag tggtttttca acaaaccaca gagacgcgga
     5041 gacacggagg tctaatcact cttacgcaat cttacttcaa cgtcgccaaa tccgatcacg
     5101 tcaccgtctt cgatagctcg cgattcaccg taagcaatac gtctgccgtt gatgtatgta
     5161 ccattggtcg atcctgtatc cgccacgagc agggtgcctt cggaagtcat caacaaagcc
     5221 gcatgaatct tcgacacgct ggcgtcgttc agtgttaact cgttatcgga agcgcggccg
     5281 acgttcagcc gcttgccgcc gggtttgaac actaatggta cttcgcgtgt tccattttgt
     5341 tgcgtgatcc gggcaacgac ttcaatgtcc ggcattggcg gagcggcgct cggaatagcc
     5401 actccggatt tcgccgcgcg acgttcttcg tcctgtcgtc gcaggtcttc ctcaaactcg
     5461 ccaaaggtcg gatcgacgga aatgccggtt gtgaaaatat ctacttcggc ttccacgctg
     5521 actggaccaa gagtgcgata gcgatggtcg ttgatgtgat cgatcgcagc cgcgagtact
     5581 tcgtgcttca actcatgaag aatttccggc ggcgcttcgg agtgtgtgcc ccactcgact
     5641 ttgagaatta ggtgatgggg cgcgatccgt cctttggcgc cttcgtcgcg aacgcgctcg
     5701 tcgatcaact tcttcatgcg ttcgatgagt ttggaagtcg tgagtccggt gcgcggctcc
     5761 accgtgcggc caaaccggcg atcgacgacg tcgcccatcc cttcgagcac gcgccgcagt
     5821 atgcgttcgg gcagtgcctc ttcgcgccgc ttttttacag ccgggtcttc tttaggcttt
     5881 tcggtcgggt cgtctgagtt aatcattgtc cgtgtgtacg aattctagcc tatcggtgac
     5941 tcaaaaatct tgtccgccgc caccaagatg acttacagta gcagtctgca gccctaactc
     6001 acacagcttg tagacaaatc ctggaggttc accaaatgct cgaacaactg tgggatagtt
     6061 tcgtgctctt gattccggta ttctttctgc tgacgatgat tttcggcgcg ggcttctaca
     6121 tcggtcgcgt cagcaagaga gaatagacac aggcactcgg cggctttgtg ctttgtacaa
     6181 agctcaaaac accccccttt gattttccat tcaggtttgc tactctttcg acacccccac
     6241 tcaccctttg attgccccga catgtagaac attcttggag tgcatgcact ggcattcctt
     6301 gggaagcaac tgaaggagct aactatgaac cgtaagagat ttcacccatt cgctgttgtg
     6361 tggggcgcag cccttgtgct cgcggtgatg ccggtagcgt tgggtcagtt tacaaacaac
     6421 caacaaacgt cgaccgctca aaacacggcg ctccgttccg tgcctgccgg cgcgaagatg
     6481 aagttcagag gcgtggtgat cggccgcgac gctgacgttt tcacgattcg ggaccggagt
     6541 cgcgccgact atcaggttct tataactgac aatacgagca tcaagaccca cggcggtttc
     6601 ttccgcagcg gcaaaaaata tccagttacc gacattcttc gcggactgat cgtggaggtt
     6661 gagggacgcg gcgataatca aggtcaattg gtcgccgaca agattcgctt cagcgaatca
     6721 gacatgcgcg ccgcaatgac gactgacgct cgcgtcggtc cggtcgaggc taacatggag
     6781 cgtgtcagcg gccagatgga cgagctctat gccgtcgcag ccgaagccag agctgaagtt
     6841 aaagccgtga atgaacgcgt ttccgcactc gacgattacg acgtgcagga aacagtgtcg
     6901 gtgactttca ggacgaacag cgccgtgctc tcgcctgaag caaaggcgca acttgacgcg
     6961 ctcgccgcga aaacggccgg tgcaaaagca ttcatgatcg aagtagccgg acacacggat
     7021 tccaccggca gcgacgcgaa gaacttccgg cttagccgcc aacgcgcaga agccgtcgtt
     7081 cagtatcttg ctgttaatca caggattccg ttacgccgtt ttgtcacgcc aatgggctat
     7141 ggcaagaccg acgctgtggc tgacaacaca acacgtgaag gtcgttcgca gaaccggcgg
     7201 gtcgacgtga agatgatcgt caatcgcggc ctgaaccaga cggggacaac cccaacatcg
     7261 tcgtcggcgc ggccctaacg cgcaatttgc ctggaacctg ggacagcagg ccgctcgcct
     7321 gcaaggtttt gaaacagtcg ttgtcgtcag tttggcagtc gacatgcctg cggtcccagg
     7381 tgacaggttc ttaaccgaat agcgccgtga cctgctttgc ctcaaaagtc ctctctcatt
     7441 ctcaacgggc tttagcccgg tgcaggagca gtgaagacgg aggaaaccgt ttcaacggtt
     7501 ttccggtgat tcgccagcga aaccgttaaa acggtttctc aaaaccgcgt ggcggtaaca
     7561 ccgggctgaa gccggtgaga atgaaagaag acttttgagg caaagccgcc gtgaccatat
     7621 tcgtgacgaa atatcgccac gcagtacacg gtttccgcgt ctgagacccg ctttcgttca
     7681 caaacctggc cataacccct caaaatccgc catttttccg tgggctttag tggcctcgcg
     7741 tttgcaaagt ttttccctca tgacgaatag tgaggtcttc ccaattctgc acggcggcac
     7801 aacagccgca aagaaaactg agccggcgcc gaactccgat ctcgcgttgg ccatggccgc
     7861 cgggaaaggc gacatggagg cgttcgagaa gttgtatgaa cggcacagcc gtcgggtcta
     7921 ttcgctgtgt ctccgcatga cgcaaaatgg cgctgaggct gaagacctgg cgcaggaggc
     7981 gttcatccag ctctttcgca agatcggaag ctttcgtggc gactcggcgt tcaccacgtg
     8041 gctccatcgg ctggtcgtaa atcagtgttt gatgcacttc cgcaagcgga gcgtcaaact
     8101 ggagaaaact accgaagaag gtgaaacacc ggtgcagatc gtgcgtggca ccgagaaccc
     8161 gaacgctatg ccggtcatgg atcgcattgc actcgacaat gcgttgagac agcttccacc
     8221 gggatatcgc accgtgttcg tgttgcacga cgttgaaggt cacgaacacg aagagattgc
     8281 gaggatgctt ggggtggctg tgggcacttc gaaatcacag ttgcacaaag cacgaatgaa
     8341 actg
//
LOCUS       DASTEY010000183         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_333609, whole genome shotgun sequence.
ACCESSION   DASTEY010000183 DASTEY010000000
VERSION     DASTEY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333609"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFO72_03265"
     CDS             complement(<1..584)
                     /locus_tag="VFO72_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478337.1"
                     /translation="MAEDLANKLPETDGEKLTLILTTVQALTGRVESAESLRPLLHKV
                     VDDISQLREGQIELRGDISQLREGQIEGRAEILQLREGQIELRGDISQLQEGQIELRK
                     DVSQLHEGQNELRADVSTLREGQNEMRADISKLREGQNEVRADISKLREGQIESRTDI
                     LQLLEGQNELRADISKLREGQNEVRADISKLREG"
     gene            complement(712..1605)
                     /locus_tag="VFO72_03270"
     CDS             complement(712..1605)
                     /locus_tag="VFO72_03270"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET9478338.1"
                     /translation="MEIRKVGVLGCGLMGSGIAQVAAAAGYQTVVKEVSEQLINKGFG
                     GIEKSLAKFAEKGTITTEQQSEIRGRLSGTTSFDALADCDIIIEAIIENLEEKRSTYR
                     QLDAICKPETIFASNTSSLSITEMMTATSAERQQRFIGLHFFNPVPIMKLVEVVRTIL
                     TDEAAYEQALGFAKSLGKVPVRAGDKTGFIVNRLLVPYMLDAIRALEEGVGSIVDIDN
                     AMKLGCGYPMGPFTLGDFVGLDTTYYIAEIMFNEFREKRFAPPPLLKRMVLAGLYGRK
                     TGRGFYDYTKDAKNPTPMNLV"
     gene            complement(1628..2905)
                     /locus_tag="VFO72_03275"
     CDS             complement(1628..2905)
                     /locus_tag="VFO72_03275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9478339.1"
                     /translation="MCRSCGAIVGAGEPQCASCGTPTGITPEQLQAYPTADRETIRFA
                     RAILSRPYMFTVVLLMTNLFVFMLMWQSSGLLSQVLWQGFPEPVLVAYGAKLNYLINA
                     PFHQWWRFITPMFVHINLPHLLVNMYSLWIVGPYVEKLYGSAKFIVFWVMTGIVGVVA
                     SYLTVQPQLATSSFGRFLFKNLDVPSAGASGALFGLVGVLFVFGIKFRRELPEGFKRA
                     FGTGMLPIIIINLFIGYMGRGFIDNAAHLGGLLSGAALATVVEYRRPGERRGLAAAWR
                     ILQALVLSLIVIGFYQAARNFNRSLQLAEGHAMLTQPQQIFVRYVAAMNLGIEKVEAI
                     IENHDLRDVTTVADRAVNAQAPDARAAELRDQLVRILGTVANAAATASPPAGDGPRRP
                     AALDQKIVQEFAQWKTEYDEWRKGAVQTYTTPG"
     gene            complement(2960..>3279)
                     /locus_tag="VFO72_03280"
     CDS             complement(2960..>3279)
                     /locus_tag="VFO72_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9478340.1"
                     /translation="FLEHANEEQGHADSIAQRIVQLKGEPNFNPDGLLTRSHAEYVEG
                     TTLTDMIKEDLVAERIAIDSYREMITYFGDDDPTSRRLMEEILAVEEEHADDLVSLLE
                     KMG"
BASE COUNT          727 a          965 c          816 g          771 t
ORIGIN      
        1 tggccttcgc gcaactttga gatgtcggca cgcacttcgt tttggccttc gcgcaacttt
       61 gagatgtcgg cacgcaattc gttttggcct tcgagcaact gtaaaatgtc ggtacgactc
      121 tcgatttggc cttcgcgcaa ctttgagatg tcggcacgca cttcgttttg gccttcgcgc
      181 aactttgaga tgtcggcacg catttcgttt tggccttcgc gcaacgttga gacgtcggca
      241 cgcaactcgt tttggccttc gtgcaactgt gagacgtcct tacgaagctc gatttggcct
      301 tcctgcaact gtgagatgtc gccacgaagc tcaatttggc cttcccgcaa ctgtaagatt
      361 tcggcacgac cctcaatttg gccttcccgc aactgtgaga tgtcgccacg aagctcaatt
      421 tggccttccc gcaactgtga gatgtcgtct accaccttat gcaagagcgg acgtagtgac
      481 tccgcactct cgactcgacc cgtcagtgct tgcacggttg tgaggataag cgtcagcttt
      541 tcgccgtcag tttctggtag tttgttcgcg aggtcttcgg ccataaatac tctcctttgc
      601 gagtgattgt agcactcgca aaggagaacg gagcaggaaa gtgttgtcga aaatggctat
      661 ttgtttgcga aaacggctaa acgttcgaca ggattttggg attcacacga gtcacaccag
      721 gttcatgggt gttgggttct tcgcgtcttt cgtgtagtcg taaaacccgc ggcctgtttt
      781 gcgcccgtac aatcccgcca aaaccatccg cttcaacaac ggcggcggcg caaagcgctt
      841 ctcgcgaaac tcgttgaaca tgatctccgc gatgtaatag gtcgtgtcga gtcccacaaa
      901 gtctccaagt gtgaacggcc ccatcggata accacatccc aacttcatcg cgttatcaat
      961 atcaacgatc gaacccacgc cctcttccaa cgcgcggatc gcatccagca tataaggcac
     1021 cagcaaacga ttcacgataa atcccgtctt gtctcccgca cgcaccggca cctttcccag
     1081 cgacttcgca aaaccgagcg cctgctcgta agcagcctcg tccgtcagaa tagttcgcac
     1141 tacctccacc agcttcatga tcggcacggg attgaaaaag tgcagaccga taaaacgttg
     1201 ctgacgttcc gccgacgtgg cggtcatcat ctcggtaatt gaaagcgacg aagtgttgga
     1261 agcgaagatc gtctccggtt tacaaatcgc gtcgagctgc ctatacgtgc tgcgcttctc
     1321 ttcaagattc tcaattattg cttcgatgat aatgtcgcaa tcagccaggg cgtcgaacga
     1381 cgttgtgccc gacaggcgcc ctcgaatttc actttgctgt tccgttgtga tcgttccttt
     1441 ttccgcaaac ttcgccaacg acttctcaat tccgccaaaa cctttattaa ttaactgctc
     1501 cgaaacttct ttcacgaccg tttgatatcc cgccgcggcc gcgacttgcg ctattcccga
     1561 acccatcaag ccacaaccca aaacacctac tttcctgatt tccatgtcgt tctcctaaaa
     1621 aatattttta tcctggcgtc gtgtaagttt gaactgcgcc tttcctccac tcgtcgtact
     1681 ccgtcttcca ctgcgcaaac tcctgaacaa tcttctggtc gagcgcggcc ggacgccttg
     1741 gtccgtcacc agcaggtgga ctcgcagtcg ccgctgcgtt tgccacggtc ccaagtatcc
     1801 tcaccaattg atcgcgcaac tcggccgcgc gcgcatcggg agcctgcgcg ttgaccgctc
     1861 gatcagcgac ggtggtgacg tctcgaagat cgtgattctc gatgatggcc tcgacctttt
     1921 cgatacccag gttcatcgcg gcgacgtagc ggacaaagat ttgttgcggt tgggtcaaca
     1981 tcgcatgacc ctcagcaagt tgcagcgaac ggttgaagtt tctggccgcc tggtaaaacc
     2041 cgattacgat cagcgaaagc accagcgcct ggaggattcg ccacgccgcc gcaagaccac
     2101 gccgttcccc tggccggcga tattcaacga ccgtcgccag cgcggcaccc gacaacaacc
     2161 cgccgagatg cgcggcattg tcgataaacc ctcgacccat gtatccgatg aataagttaa
     2221 tgatgatgat cggcagcatt cccgtcccga aagcccgctt gaatccttcg ggaagctcgc
     2281 gccgaaattt aatgccgaat acaaaaagca cgccaaccaa accgaagaga gcacccgacg
     2341 cgcctgccga gggcacgtcc aagttcttaa acagaaatct gccgaacgaa ctggttgcca
     2401 gttgcggctg cacagtcaaa tagcttgcca ccactccgac tatcccagtc atcacccaaa
     2461 aaacgatgaa cttcgccgaa ccatacaact tctcaacata tggtccaact atccagaggc
     2521 tgtacatgtt aaccagcagg tgcggcaagt tgatatggac aaacatcggc gtgatgaaac
     2581 gccaccattg atgaaaagga gcgttgatga ggtaattcag cttcgcgcca tatgcaacca
     2641 gcaccggttc gggaaacccc tgccagagca cttgcgacaa cagcccgctc gactgccaca
     2701 tcaacataaa cacgaacagg ttggtcatga gcagcacgac ggtgaacatg taaggacggc
     2761 tgaggatggc tcgcgcgaat ctgatcgttt cgcgatcggc tgtggggtag gcctggagtt
     2821 gttctggtgt gatgccggta ggcgtgccgc aggacgcgca ttgcggttcg cccgcgccta
     2881 cgatcgcccc acaactgcgg cacattgttg gccgaccggg gtcagtttgg tttgactggc
     2941 tttgagacaa gcgctgccgc taacccatct tttcgagaag actgactaga tcgtccgcgt
     3001 gctcttcctc gacggcgagg atctcttcca tcaaacgccg tgaagtcgga tcgtcatcgc
     3061 cgaagtaggt gatcatctcc cgatagctat cgatggcgat acgttcggcg accaggtctt
     3121 ccttgatcat gtcggtcagc gtagtgcctt cgacgtattc agcgtgactc cgcgtcagca
     3181 gtccgtcagg attgaagttt ggctcgcctt taagctgaac gatgcgttgc gcgatggagt
     3241 ccgcgtgacc ctgttcttcg ttcgcgtgtt caagaaact
//
LOCUS       DASTEY010000184         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1108094, whole genome shotgun sequence.
ACCESSION   DASTEY010000184 DASTEY010000000
VERSION     DASTEY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108094"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFO72_03285"
     CDS             complement(<1..203)
                     /locus_tag="VFO72_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478341.1"
                     /translation="MTTSNKVTSSKWSALIADDEPAARRGVRQLLAAFPEFAVAGECR
                     NGAEVLAALDVSAPDVVFLDIQMP"
     gene            complement(172..1371)
                     /locus_tag="VFO72_03290"
     CDS             complement(172..1371)
                     /locus_tag="VFO72_03290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9478342.1"
                     /translation="MRSLMLRAMSGGRYCGPVRFSPRLRPWMIVSAAWILPAVFAAIN
                     RIVQAHLRGWEPATIRDVLFEGGDWFLYALLTPGVFAVSKRWPLTRPHLVRRALIHLG
                     FSVLFCVAWASCGQLLRLVLALIFAPETVGGSEFWRQFGVEWLSWIFITLPFGVAVYL
                     CVVGIEHAIRYFVEVREREVQLARLSEQLAGARFAALQAQVNPHFLFNTLNTIAVLVR
                     DDDRQGAVRIVEHLSEMLRRTLSRHRANQVSLGEELELVRQYVAIEQARFSDRLRPEF
                     RVPDSLLSALVPSFALQHLVENAIRHGIARAPDAGILIVAARRDGDVLEITVSDDGVG
                     IDPDAPITKGHGIDNTRERLRALYGENASLEIVRHAEGGTIATLRVPYLESTANTDDN
                     EQQSDVE"
     gene            1453..1854
                     /locus_tag="VFO72_03295"
     CDS             1453..1854
                     /locus_tag="VFO72_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9478343.1"
                     /translation="MTIKPTPKIECEQHHTGLLTSNLDAAIDFYTNKLGFWLAFKWGE
                     PPTMAGVNLGHVQLFLEHGEPSPKGCYLYFVINDADAFYEFHRNNGVEIVQPIADRDY
                     GIRDYSVRDLHGYVLSFGHRLPGNCEEGDHS"
     gene            1851..>2488
                     /locus_tag="VFO72_03300"
     CDS             1851..>2488
                     /locus_tag="VFO72_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478344.1"
                     /translation="MKLLLISITSFALGAVCFAGVTQPDRAADAEAIRAHIGSIFQAF
                     IDGDEQKIYDTHSEDWRGLLEGTPTPIKGIDEYMRANGIEWPKPANAPKSHPYYPPGT
                     GYKMNDFDVHFYTPELAVVNFIGEFVRKDGNTTTTLRRFRIMDIYAKRKGSWIQVASH
                     TAVDPAWRDEQTSQPRNLTPEVRQQILEKREAVWKAWFANDRPTLEKLIPEEA"
BASE COUNT          611 a          808 c          635 g          434 t
ORIGIN      
        1 ggcatctgaa tatcgagaaa cacgacatcc ggcgcagaca cgtcgagcgc cgccaggact
       61 tcagcgccat tgcgacactc tcctgccacg gcaaactcgg gaaacgccgc cagcagttgt
      121 cgcacaccgc gccgcgccgc cggttcatcg tcggcaatca gtgcactcca tttactcgac
      181 gtcactttgt tgctcgttgt catccgtgtt tgccgtcgat tcaagatacg gcactcgcag
      241 cgtcgcgatt gtaccgcctt cagcgtgccg cacgatctcc agtgaagcgt tctcgccata
      301 caacgcccgc agccgctcgc gcgtgttgtc gatgccgtgg cctttggtta tcggcgcatc
      361 cggatcgatc ccgacgccat catcgctgac cgtaatctcg agcacgtccc catcgcgtcg
      421 cgcggccaca atcaaaatac cggcatcagg agcgcgcgcg atgccgtgcc gaatcgcatt
      481 ctcaaccagg tgctgcaacg cgaaactcgg aaccagagcc gacaacagcg agtcaggcac
      541 tcgaaactcc ggccgcagac gatccgaaaa ccgcgcctgt tcgatcgcca cgtactgccg
      601 caccaactcc aactcttcac ccagcgaaac ctgatttgct cgatgccggc tcagcgtgcg
      661 ccgcaacatc tcactcaggt gctcaactat tcgcaccgcc ccctgacgat cgtcatcacg
      721 gacgagcacg gcaatcgtat tcagggtatt gaaaagaaag tgcggattga cctgcgcctg
      781 caatgccgca aagcgcgcac cggcaagttg ctccgagagc cgtgccaact gcacctcacg
      841 ctcgcgcacc tcgacaaagt atcgaatcgc atgctcgatc ccgacgacgc acagataaac
      901 agcaacacca aacggcaacg tgatgaagat ccaactcaac cactcgacgc caaactgtcg
      961 ccagaattca ctgccgccaa ctgtttccgg cgcgaagatc aacgccagga ccaaccgcag
     1021 gagctgcccg caactggccc atgcgacaca aaacaaaaca gaaaacccca gatggatcag
     1081 cgcacggcgc acgagatggg gtcgcgtcaa cggccatctc ttcgagactg caaaaacacc
     1141 gggcgtcaac agcgcgtaaa gaaaccagtc gccgccttca aacagcacgt cacgaatagt
     1201 cgccggttcc caacctcgaa ggtgcgcttg aacgatgcgg ttgatcgcgg cgaacaccgc
     1261 cggaaggatc caggcggctg acacgatcat ccagggccgc aagcgtggcg agaatcgcac
     1321 cgggccacag tatcgcccgc cactcatcgc gcgcaacatc aatgaacgca cgccgaccgt
     1381 tcgcgtccaa acatagaccg ctcgcgtatt cttcgttgtc gttcaaacaa ggccaggcgt
     1441 aggatgacgg ccatgacgat caagcccacg ccgaagattg aatgcgagca acaccacacc
     1501 ggtttgctga cgagcaacct cgatgcggcg atcgatttct acacgaacaa gctcggcttc
     1561 tggctggcgt tcaagtgggg cgaacctccg acaatggccg gagtcaacct gggccatgtc
     1621 cagttatttc tagaacacgg cgagccatcg ccaaaaggtt gttatctcta ctttgtgatt
     1681 aacgacgccg acgcattcta cgaatttcat cgaaacaatg gcgtcgagat cgtccaacca
     1741 atcgccgacc gcgattacgg tatacgcgac tacagcgttc gcgacctgca tggatacgtg
     1801 ctctcgttcg gacatcgtct gcccggcaac tgcgaggaag gagaccattc atgaaactgc
     1861 ttctcatatc tatcacgtca ttcgcccttg gcgcggtgtg tttcgccggc gttactcaac
     1921 ccgatcgcgc cgccgacgcg gaagccattc gcgcgcatat cgggagcatc ttccaggcct
     1981 tcatcgacgg cgacgagcag aagatctacg acacgcactc ggaagactgg cgcggactcc
     2041 tcgaaggcac gcccactcca atcaaaggca tcgacgaata catgcgcgcg aacggtatcg
     2101 agtggcccaa gcctgccaat gcgcccaagt cacatcctta ctatccaccg ggcaccggat
     2161 acaagatgaa cgactttgac gtccacttct atactccgga acttgcggtg gtgaacttca
     2221 tcggcgaatt cgttcgcaaa gacggcaaca caacgaccac cctgcgacgg tttcgcatta
     2281 tggacatcta cgcgaaacga aaaggaagct ggatacaagt cgcttcgcac acggccgtcg
     2341 atcccgcatg gcgcgacgag cagacgtcgc aaccgcgtaa tctcacgccg gaagtacgcc
     2401 agcaaatcct ggaaaaacga gaagcggttt ggaaagcgtg gttcgcaaac gatcggccaa
     2461 ctcttgaaaa gttgattccc gaagaagc
//
LOCUS       DASTEY010000185         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1372018, whole genome shotgun sequence.
ACCESSION   DASTEY010000185 DASTEY010000000
VERSION     DASTEY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372018"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFO72_03305"
     CDS             complement(<1..793)
                     /locus_tag="VFO72_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478345.1"
                     /translation="MVRYAMIPAMPSAISLNTPIEDLHNFRIARLGDQLSHKLATALA
                     SHANKRHAADVTVEDLLNYLPMRYEDRSHPAAIRDLSDGLEASLELLVHRAHGYEVRK
                     GYGRSRLFIFEVTAYDAARTGRQVIVWWFVSGARAYEIVKYYTNKLAPGTRFITFGRW
                     EWNATKSTYKLQLHRPADELEILPSFEQQTLTGNGEVDLETSEENVPDPSLALIHVGR
                     RVPVYRKLGPFNSKRVREVIHAVLSSLDPQTIDETLPPDLLQRVRL"
     gene            824..1237
                     /locus_tag="VFO72_03310"
     CDS             824..1237
                     /locus_tag="VFO72_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478346.1"
                     /translation="MSNERASELTRDSLKELRHALLRLHKLLLDLERRDFERARGRIS
                     NSYEFLQLVLKDPWFDWLHRLSELIVQIDESLDTRETETPMTEEDAKALIERAKTLLA
                     PSESGTQFQKNYFLALQQSPDVVLLHSEVMRLLGR"
     gene            complement(1564..1986)
                     /locus_tag="VFO72_03315"
     CDS             complement(1564..1986)
                     /locus_tag="VFO72_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HET9478347.1"
                     /translation="MAYPEIMIRPMREDLTRLGVEELRTAEAVDETIKNSKGTLMVVV
                     NSICGCAAGKARPGVALALQHDVKPDKVATVFAGADIEATERARSYFTGYGPSSPSIA
                     LLKDGELVYMLERWQIEGRDASQIAGELTKAFEQHCAA"
     gene            complement(2514..2999)
                     /gene="tsaE"
                     /locus_tag="VFO72_03320"
     CDS             complement(2514..2999)
                     /gene="tsaE"
                     /locus_tag="VFO72_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET9478348.1"
                     /translation="MNSTPAHVSEGQSMLPEGEFNSSSPEETFNYGHQVGARLEGGEI
                     LLLSGPLGAGKTLFVKGICAALGVAEEDVTSPSFTLVNPYAGRLRLYHIDLYRLDEGV
                     SAAHAVDLDELLSDERAVIVIEWAERMGNYPLPNNVWRIEISGDGDSPRHIRICRKRL
                     N"
     gene            complement(3009..>3293)
                     /locus_tag="VFO72_03325"
     CDS             complement(3009..>3293)
                     /locus_tag="VFO72_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9478349.1"
                     /translation="DGRVFINPTGNAGLGTAGSGDTLTGLISGFLAQAYGTLRDAADP
                     LAATIAAVYIGGLAGDLAARKLGMRTMVASDIREHFSEAIRLLDLEGEQP"
BASE COUNT          711 a          891 c          942 g          749 t
ORIGIN      
        1 tcaagcgaac gcgttgcagc aggtcgggcg ggagtgtttc gtcgatcgtt tgcggatcga
       61 gagaggacaa aacggcgtga atcacctcgc gcaccctttt cgagttaaat ggcccaagct
      121 tccggtaaac tggcacacgg cggcccacgt gaatcagagc cagtgaagga tcaggaacgt
      181 tctcttccga cgtttcaaga tccacctcgc cgtttccggt aagagtttgt tgttcgaaag
      241 agggcaggat ttcgagctca tcggccgggc ggtgcaactg tagtttgtag gtacttttcg
      301 tcgcgttcca ttcccagcgg ccgaatgtga tgaagcgagt gccgggtgcg agtttgttgg
      361 tgtagtactt gacgatctca taagcgcgag cacccgaaac gaaccaccac acgatcactt
      421 ggcgtccggt tcgggccgcg tcgtaagccg tgacctcaaa gatgaagagt cgcgagcggc
      481 cgtatccttt tctgacttcg tagccgtgcg cgcggtgcac gagcagctcg agcgacgcct
      541 ccaaaccgtc cgacaaatct cttatcgccg ccggatgaga acgatcttcg taacgcatag
      601 gcagatagtt gagtagatct tcgaccgtca cgtcagcggc gtgtcgtttg tttgcgtgtg
      661 acgcgagcgc cgtagcgagt ttgtgtgaga gttgatctcc caggcgcgcg atgcggaagt
      721 tgtgaagatc ctcaatcgga gtgttgaggg agatggcgct gggcatcgcc gggatcatag
      781 catagcggac catcggatat acttggcggc aacagggggc tttatgagta acgagagggc
      841 gtcggagcta acccgtgatt cattgaagga actgcggcat gcactattgc ggttgcacaa
      901 actgttgctc gatttggagc ggcgggattt cgagcgcgcg cgcgggcgga ttagtaacag
      961 ttatgagttt ttgcagttgg tgttgaaaga cccgtggttt gattggttgc accgtttgtc
     1021 ggagctcatc gttcagatcg atgaatcact agacacgcgc gaaaccgaga cgccgatgac
     1081 ggaagaggat gcgaaggcgt tgatcgaacg tgcgaagacg ctcctggcgc cttcagaatc
     1141 gggcacgcag tttcagaaga actactttct cgcgttgcag caatcacccg atgtggtgct
     1201 gctgcattcg gaggtgatga ggctgttggg gcgttagaac ttggtacttt gtacaactag
     1261 aatcctttcc cagggacgct catctgtcat cctttcattt cactcacgac ggacgtgctc
     1321 ttccctggga ccgcaggcgt ctcgcctgca tgacacgaga gcgcccgatt cgtcagttgc
     1381 aggcgtctcg cctgcatgac acgagagcgc ccgattcgtc agttgcaggc gtctcgcctg
     1441 catgacacga gagcgcccca tccgtcaccc tgcaggcgag acgactgcgg tcccagggga
     1501 agagcgcgtc ccgaaaaatc ggtgatgggt tcaaactgca tcactactca aagctctaag
     1561 cactcatgcc gcgcagtgct gctcgaacgc cttcgtcagt tcgccggcga tctgagatgc
     1621 gtcgcgccct tcgatttgcc atctctcgag catgtaaacc aactcgccat cctttaacag
     1681 cgcaatcgaa ggtgaggatg gtccatagcc ggtaaaatag ctgcgggcgc gttcggtcgc
     1741 ttcgatatcc gcgccggcga agacggttgc tactttatct ggcttaacgt cgtgttgcag
     1801 cgcgagcgcg acaccaggac gtgcctttcc ggcagcgcac ccacagatcg aattcacgac
     1861 caccatcaaa gtacctttac tgttcttgat cgtttcatca accgcctcag cggtcctcaa
     1921 ctcttccacc cccagacgcg tcaaatcctc gcgcatcgga cggatcatga tttcaggata
     1981 agccatcgaa agttgctcct ttgtaagaat ttataatgtt accacacgag ttgcgcctgc
     2041 cagagtacgc taactcgatc tgactgccga gcacgctgct accagccatt cttcgtagtg
     2101 gctttctctc attctcaccg ggctttagcc cggtgttcgt ctgctcgatc tgagtaaccg
     2161 ttttaacggt tttcgagacg acccagacct aaaccgttga aacggtttct gagagcgacc
     2221 aggctttttc accgggctga agcccggtga gaatgaaagg atgcccttga cggggagtgt
     2281 acagcgagga ctgcatgtct cttaaaacca cttatgacgt cgcacaagca gatctagcag
     2341 agttgctgga tgaggtgtcc gaaaaccaac agagggtcat tattactcgg cgtggtaagc
     2401 gagatgtagt aatgatccgg gctgacgaac tatccggtct gatagaaacc gcgcaccttc
     2461 tttcgtcacc aagaaacagt gaacgcttat tggtcgccta acgccgagcc aagtcaatta
     2521 agtcttttcc ggcaaatacg gatgtggcgg ggggagtcgc cgtcgccgga gatttcgata
     2581 cgccagacgt tgttgggtag agggtagttg cccatacgct ctgcccactc gatcacaatg
     2641 acggcgcgct cgtcggagag taattcgtcg aggtcgactg cgtgtgctgc gctcacgcct
     2701 tcatcgagac ggtatagatc gatgtgatag agccgaaggc gtccggcgta cgggtttacc
     2761 aacgtgaacg aaggactggt tacgtcttct tcagcaacgc cgagggccgc gcagattcct
     2821 ttcacgaaaa gcgttttgcc tgcgccgagt ggaccgctca acaacagaat ctcgccacct
     2881 tcaagccgcg ctccgacttg atgaccgtaa ttgaacgtct cttccggcga ggaactgttg
     2941 aattcgcctt cgggaagcat cgattggcct tcactcacgt gtgccggcgt actgttcatg
     3001 tctccagctt acggttgttc accttccaga tcgagcaaac ggatggcttc actgaagtgt
     3061 tcgcgaatgt cggaggcgac catcgtgcgc atccccagtt ttcgtgccgc caaatcaccg
     3121 gcgagaccac cgatgtagac cgcagcgatt gtagcggcaa gaggatccgc ggcatcgcgc
     3181 aacgttccgt aagcctgcgc gagaaaaccg gagattagac ctgtgagtgt atcgccagag
     3241 cctgcggtgc cgagtcctgc attgccggtt ggattgatga acacgcgtcc atc
//
LOCUS       DASTEY010000186         6964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_844628, whole genome shotgun sequence.
ACCESSION   DASTEY010000186 DASTEY010000000
VERSION     DASTEY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844628"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2123
                     /locus_tag="VFO72_03330"
     CDS             <1..2123
                     /locus_tag="VFO72_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9478350.1"
                     /translation="HLLTVVVFITSVLSTNGALKAQSNEGAAPNKPPMAEKKSKITTI
                     HGEQLVDDYFWLREKTNPAVLAHLQAEDAYAEKVMKPTSALQEKLYKEMLSHIKQTDT
                     NVPYRWGNYFYYTRTEEGKQYPIYCRKRGSVDAPEEIVIDQNELAKGQKFMSIGLFVP
                     SDDGNLLAYTTDNTGYRQYTLHIKDLRNGQLFSERIERVNNLAWATDNKTFFYVTEDA
                     VTKRNDKFFRHVLGTDKTDLIYEEKDELFDIATGRSRDKAVIFVEAFSKTSTDVRYLP
                     ADDPNASLKVILPRQPEHEYDVDHRGDLFYIRTNKGAKNFRIVTAPVSDPSEKNWKEF
                     VAHRPQVKVDAISMFADHAVLSEWENGLQQLEVIDFKTNKRKRIEFPEPVYAAGLSSN
                     REFNTTVVRYAYNSLVTPTSVFDYDMNSGKSTLLKQQEVPGGFDRTNYKSERVFANAS
                     DGTKIPISMVYRKGAKLDGSAPLLLYGYGSYGASIPPTFSSNRLSLLDRGVIFAIAHI
                     RGGGELGEEWRQAGRMMNKMNTFTDFIASAETLVKMKYTSSDRLVIEGGSAGGLLMGV
                     VSNLRPDLFKAVVSHVPFVDVLNTMLDATLPLTTSEYIEWGNPNEKPAYEYMKKYSPY
                     DNVAKKNYPATLVKVSLNDSQVPYWEGAKLVAKLRAMKTDNNPLLLKVNFGAGHGGAS
                     GRYDALKETAFNYAFMLWQMGISE"
     gene            2209..4677
                     /locus_tag="VFO72_03335"
     CDS             2209..4677
                     /locus_tag="VFO72_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9478351.1"
                     /translation="MIGSTLGNYKILEKLGEGGQGTVYKAIDSKLGRTLVIKVLPAEL
                     TSKEANLRRFEREARLASALDHPNICTIFDLNEINGIHFIAMQYIEGRNVRQLVNGRP
                     LSLESALSITLQTAEALSAAHSRGIIHRDIKAGNVMVTPSGQVKVLDFGLAKLLDEDA
                     ARTSGIHHTEITEVGIPYGTATYAAPEQARGDRVDSRADIFSTGVLLYEMLTGTWPFQ
                     GQTAVEVRHAVLHEEPKPISQMRPGRVPARLQEIVDKALAKDPRNRYQKISYFAEDLR
                     AVIRELGMDSLPGIDESIAPVAPKHLTPRNPVTRALRWLSGAKTAESASGGSGKSPTR
                     ARDAHESPLTSLGDRDRKSVAILPFKNVGNEREIDFYQFSLADAVITELARVRSLVVR
                     PSSVIVKYQNKTVDPADAGRELRVDAILTATFLRAGDRLRVTAQLLDVRTSEILWSER
                     IDADASDIIGVQDTIVQHIVEGLRLELSPDEKVQLAKGITADAAASEEYLRGRDCLAT
                     FIYHTIEREHLDSSIEHFRRAIEIDPQFALAYSALGSAYVNRVLKGLGQSGDHEKAQR
                     AFHKALALDPKLLEARRHMVFIYLTDGQKQKAREEIELLRREYPNDSGVQFVRGVVAR
                     LDGDYDVALRSFDRMVKLNPEERVLASYNRARIFMYQGQYDEALRELDEGAALESEHP
                     VIKVFRARVLFYRGEIDEATQILQQVLQRHPQMDGIRPILAICLSAQGQHTKANEQLT
                     QKVRQVAEADYDISYWLASAYLLQGRQVEALRWLETAIQLGNENHRWFESDPNWTDMH
                     QDQRFMELMEGIKNQKAAARDQTA"
     gene            4674..5468
                     /locus_tag="VFO72_03340"
     CDS             4674..5468
                     /locus_tag="VFO72_03340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9478352.1"
                     /translation="MRTFVVGDIHGRCAQLLNLLDMLPRDPQSESLVFLGDLIDRGAD
                     APGCVDHILKLRRENPERVICLRGNHEQMLMDFLDGHNSIWLTPVTGGERTFEQYTGL
                     PVRARTEADLDEMRRVLEQSLPSEHLEFMQSLPFYYEDEHAIYVHAGLDDGKHPSESS
                     PMSLLWMRDMDFYRNYRGKPCVFGHTPTPLLPLRGRLGRHGIYISHSAVGLDTGYNHQ
                     SPLSCLSLPDFNLYQTFADGREETYQITSFIPETLREMQRRAGLAD"
     gene            complement(5608..>6964)
                     /locus_tag="VFO72_03345"
     CDS             complement(5608..>6964)
                     /locus_tag="VFO72_03345"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9478353.1"
                     /translation="RQLAQSDETMRAWMKSGIPFSTAPGTAFEYSNYGFAILGQVIAK
                     ASGKPYAGYVRDHILRPLGMTSSTYEMSGVPRDQIALGYRREDNNWKAEPILAHGSFG
                     AMGGLWTNTHDLARYVAFLMSAFPPRDEPERGPVKRSSAREMQQLWRISSGSVFRITT
                     DAPLQMSVSGYGYGLGIGQDCRFSYAVGHGGGLPGYGSLMRWLPDYGVGLIAMGNLTY
                     GGFGTLFNDTFTALHKTGALQPRVVKSSPALLAAQADISQLIIKWDDALANRIAADNL
                     FLDIPAEARKNRWQSLAGDHGTCRPATSIDPENALRGGWRMMCDRGWLDVFITLAPTT
                     TPKIQAIGVTSVLPPDGEMTKTAGAIVKLIGAWDQRALEAIAAPALNVEKVRRQIAAA
                     SAWGACKVGEPLSGSGTRNSSIRLTCEGGPLVARIALDATTRKLTNLDLVPLREQRCV
                     P"
BASE COUNT         1604 a         1981 c         1946 g         1433 t
ORIGIN      
        1 ttcacctctt gacggtcgtg gtctttataa catccgtgct ttcaaccaac ggcgcgctga
       61 aggcacaatc aaacgaagga gccgcgccga ataaaccacc aatggcagag aagaagtcca
      121 agattacgac cattcacggc gagcaactgg tcgatgacta tttctggctt cgtgaaaaaa
      181 cgaatcccgc ggtgctcgcg catttgcagg ctgaggacgc gtatgccgaa aaggtaatga
      241 aaccgacgtc cgcgctgcaa gaaaagcttt acaaagagat gctcagccac atcaagcaga
      301 ccgacacaaa cgtgccgtat cgctggggca attacttcta ttacacgcgc actgaggaag
      361 gtaagcagta tccgatctac tgccgcaagc gcggcagtgt cgacgcgccc gaagagatcg
      421 ttatcgatca gaatgaactc gccaagggcc agaagttcat gagcattgga ctgttcgtcc
      481 cgagtgacga cggcaacctg ctggcttaca ctacggacaa caccggatac cgccagtaca
      541 cgttgcacat caaagacctt cgcaacggtc aattgttttc tgaacgtatc gagcgcgtta
      601 acaatctcgc ctgggccact gacaacaaga cgttctttta cgtcacggaa gatgcggtca
      661 caaagcgcaa cgacaagttt ttccgtcacg ttctcggaac cgacaaaaca gatctgatct
      721 acgaagagaa agacgaactg tttgacatcg ccaccggacg ttcgcgcgac aaggccgtga
      781 tcttcgttga agctttcagc aaaacgtcca ccgatgttcg ttatttgccg gcagacgatc
      841 cgaacgcgtc tttgaaggtg attctgccgc gacagcccga acacgagtac gacgtcgatc
      901 atcgcggcga cctcttctac atccgcacga acaaaggtgc gaaaaacttc cgcatcgtca
      961 ccgcgccggt aagcgatcct tcggaaaaga actggaaaga gtttgtggcg caccgcccgc
     1021 aagtaaaagt cgacgcgatc agcatgttcg ccgatcacgc tgttttgtca gagtgggaaa
     1081 atggtctgca acagctcgaa gtcatcgatt tcaaaactaa caagcgcaag cgaattgaat
     1141 ttccggaacc ggtctatgct gccggtttgt catcaaaccg cgagttcaac acgacggttg
     1201 ttcgttacgc ttacaactcg cttgtgactc cgacctctgt cttcgattac gacatgaaca
     1261 gcggcaagtc gacgttgttg aaacaacaag aagtccccgg aggttttgac cgcaccaact
     1321 acaaatccga acgtgtgttt gccaacgcgt ctgatggaac aaagatcccc atttcgatgg
     1381 tctatcgcaa aggcgcaaag ctggacggtt ccgcgccgtt gttgctttac ggctatggtt
     1441 cttacggtgc ttcgattcca ccgaccttct cttcaaatcg cttgagcctg ctcgatcgtg
     1501 gcgtgatctt tgccatcgca cacattcgtg gcggcggtga gttaggcgag gaatggcgcc
     1561 aggccgggcg tatgatgaac aagatgaaca catttactga cttcatcgcc agtgccgaaa
     1621 cgctcgttaa gatgaagtac acgtccagcg atcgactggt gatcgaaggt ggcagcgccg
     1681 gtggactatt aatgggtgtg gtgagtaacc tgcgcccaga cttgttcaaa gcggtcgtct
     1741 cgcatgtgcc gtttgtcgat gtgctcaaca cgatgctcga cgcaacattg ccgttgacga
     1801 cgagcgaata catcgagtgg ggcaacccga acgagaaacc cgcttacgag tacatgaaga
     1861 agtattcgcc ttacgataac gttgcgaaga agaattaccc ggcaacgctt gttaaggtgt
     1921 cgctcaacga tagtcaggtg ccatactggg aaggcgcgaa gctggtggca aagttgcggg
     1981 cgatgaaaac cgacaacaac ccgctgctgt tgaaggtaaa cttcggcgcg ggccacggcg
     2041 gcgcctcggg ccgctacgac gctctgaagg aaacagcgtt caactacgcc ttcatgctgt
     2101 ggcaaatggg aataagcgag tagaaagaca ggattacaga attaccggat gaaatattag
     2161 acgggtcagg actaagtcct gtacattctg ctaatcctgt ctacaactat gattggctcg
     2221 acactgggga attacaagat cctcgagaag ctcggtgagg gcggtcaggg gaccgtctat
     2281 aaggctatcg attcgaagtt aggccggaca ctggttatca aagttctgcc ggccgaactc
     2341 acttcgaaag aagcgaatct cagacgtttc gaacgcgaag cccgtctcgc ctccgcgctc
     2401 gaccatccga acatctgcac cattttcgat ctcaatgaga tcaacggcat ccatttcatc
     2461 gcgatgcaat acatcgaggg acgcaacgtg cggcagttgg tcaacggccg cccgctgtcg
     2521 ctcgaaagtg ctttgtcgat cacattgcag actgctgaag cactttccgc cgcacactcg
     2581 cgtggcatca tccatcgcga catcaaagcc ggcaacgtga tggtaacgcc gtcgggccag
     2641 gtgaaagtgc tcgacttcgg actggcaaaa cttctcgacg aagacgccgc ccgcacttcc
     2701 ggtattcatc acaccgagat aaccgaagtc ggcatccctt acggcaccgc gacttacgca
     2761 gcgcccgaac aagcgcgcgg cgatcgcgtc gattcgcgtg cagacatctt ttccaccggc
     2821 gttttgcttt acgaaatgct aaccggcacg tggccgttcc agggccagac ggccgttgag
     2881 gtacgccatg cagtgctgca tgaagagcca aagccgatct cacaaatgag acccggccgc
     2941 gtgcccgcac gcctgcaaga gatagtcgac aaagcactcg cgaaagatcc gcgcaatcgt
     3001 tatcaaaaga tctcgtactt cgccgaggac ctcagagccg tcattcgcga actcggcatg
     3061 gattcgctgc cgggtatcga tgaaagtatc gcgcccgttg cgcccaaaca cttaacgccg
     3121 cgtaaccccg tcacgcgcgc gttgcgctgg ctgagcggcg caaagaccgc tgagagcgcg
     3181 tccggaggtt ccggtaagtc gccgactcgt gcgcgtgatg cacacgaaag cccgttgacg
     3241 agtttggggg atcgcgatcg caagagtgtc gcgatacttc ctttcaaaaa cgtcggcaac
     3301 gagcgcgaga tcgactttta tcagttctcg ctggctgatg cggtgatcac cgaactcgcg
     3361 cgcgtgcgtt cgctggtggt gcggccttcg tcagtgatcg tgaagtatca gaacaagacc
     3421 gtcgacccgg ccgatgccgg ccgcgagttg cgagtagacg cgattctcac cgcgactttc
     3481 ctgcgtgccg gagaccgttt gcgcgttacc gctcaactcc tcgacgtgcg aaccagcgag
     3541 attctctgga gcgaacgaat cgacgccgac gcaagcgaca tcatcggcgt gcaggacaca
     3601 attgtgcagc acatcgtcga agggttgcgt ctcgaactga gtccggacga gaaagtgcaa
     3661 cttgccaaag gcataacggc agacgccgcc gcctccgaag aatacctgcg cggtcgcgac
     3721 tgcctggcca cgttcatcta ccacacgatc gaacgcgaac atctcgactc gtccatcgaa
     3781 cacttccgcc gtgcaatcga gatcgatccg cagtttgcgc tcgcctattc agcactcggc
     3841 agtgcttacg tcaatcgcgt gttgaaggga ttgggccagt ccggcgatca cgagaaagct
     3901 cagcgggcgt ttcacaaagc actggcgctc gatccgaaac tactcgaagc gcggcggcac
     3961 atggtgttta tttatctcac tgatgggcaa aaacagaagg cgcgcgagga gatcgagttg
     4021 ctgcgccggg agtatccgaa cgactccggc gtgcagtttg ttcgcggcgt ggttgcgcgg
     4081 ctcgatggcg attacgacgt ggcgctgcgt agcttcgacc ggatggtcaa gttgaatccg
     4141 gaagagcgcg tactcgccag ctacaaccgc gcgcgcatct tcatgtatca gggccagtat
     4201 gacgaagcat tacgcgaact cgatgaaggc gctgcgctcg aatccgagca tccggtgatt
     4261 aaggtgtttc gagctcgcgt gctcttctat cgcggtgaaa tcgacgaagc aacgcagatt
     4321 ctccagcagg tgcttcagcg ccatccgcaa atggacggca tccggccgat tctcgcgatc
     4381 tgtttgagcg cgcagggaca acacacgaag gccaacgaac agttaactca gaaagtgcgt
     4441 caggtcgcgg aagccgatta cgatatctct tactggctgg cgtctgctta tctcttgcag
     4501 ggccggcagg tggaagcgtt gcgatggttg gaaaccgcta ttcagcttgg caacgagaac
     4561 catcgctggt ttgagtcgga tccaaactgg acagacatgc accaggatca gcgcttcatg
     4621 gagttgatgg aaggaatcaa gaaccaaaag gcagcagcca gggaccagac tgcatgagaa
     4681 ccttcgtcgt cggcgacata cacggtcggt gcgcgcagct cctcaacctg ctcgacatgc
     4741 tgccgcgcga tccgcaatca gaatcgctgg tgtttctcgg cgacctgatc gatcgcggcg
     4801 ccgacgcgcc gggttgtgta gaccacattc tcaaactccg ccgtgagaat cccgagcgag
     4861 tcatctgcct gcgcggcaat cacgaacaga tgttgatgga ctttctcgac ggtcacaatt
     4921 cgatctggtt aacaccggtg accggcgggg agcgcacctt tgaacagtac acgggacttc
     4981 cggtgcgcgc gcgaacggag gcggatctcg atgagatgcg gcgcgtgctt gagcaatcgt
     5041 tgccgtcaga acatctcgag tttatgcaga gcctgccgtt ttattacgaa gacgaacacg
     5101 cgatctatgt tcatgcgggt ttggatgacg gcaagcatcc gagtgagagt tctccgatgt
     5161 cgttgttgtg gatgcgcgac atggactttt accgaaacta tcgcggcaag ccgtgcgtgt
     5221 ttggacacac acccacgccg ctactgcctc tccgcggccg gctgggccgt cacggaattt
     5281 acatctcgca tagcgccgtc gggctcgaca ccggctacaa ccatcagtcg ccgctcagtt
     5341 gtctctcact gccggatttc aacctctacc agacgtttgc tgacggccga gaagagacct
     5401 accagattac ttcctttatt cctgaaacgc tgcgtgaaat gcaacgccgc gccggtctcg
     5461 cggattgact aaagcgtctt tcctgggacc gcaggcatct ttttcctggg accgcaggcg
     5521 tctcgcctgc ccgcgccatt tttcgcgctc cgcgctcagt gcaggcgaga cgactgcggt
     5581 cccaacagaa acacatcctt gttgtcttta aggtacgcaa cgttgctcgc gtaagggcac
     5641 aagatcgagg tttgtcaact tgcgcgtcgt agcatccaaa gcgattcgcg ccaccagtgg
     5701 tccaccttcg caggtcagtc gaatcgaact gttgcgcgta ccgctgccgc tgagtggttc
     5761 accaactttg caagcacccc acgctgacgc cgctgcgatc tgacgtcgca ctttctcaac
     5821 attcaaagcg ggcgcggcaa tcgcttccag cgctctttga tcccatgcgc cgatcaactt
     5881 cacgattgcg ccggcagttt tggtcatctc gccatccggc ggcagcaccg atgtgacacc
     5941 gatcgcctgg atttttggcg ttgtcgtcgg cgcaagcgta atgaagacgt caagccagcc
     6001 acgatcgcac atcatgcgcc aacccccgcg caatgcgttt tccggatcga tcgaagttgc
     6061 cggcctgcac gttccgtgat ctcctgccag cgattgccat cggttcttgc gtgcctccgc
     6121 aggtatgtcg agaaacaggt tgtcggcggc gattcgattg gcgagcgcat cgtcccactt
     6181 gatgatcaac tgcgagatat ccgcttgcgc cgctaacaat gcaggcgaag attttacgac
     6241 ccgcggctgc aacgcgcccg tcttgtgaag agcggtgaac gtgtcgttga aaagagtgcc
     6301 gaatcctcca tacgtcaagt tacccatcgc gatcagaccg acgccgtaat caggcagcca
     6361 gcgcatcaac gagccgtaac cgggcagccc accaccgtgt ccgacagcat aagaaaagcg
     6421 gcaatcctga ccgatgccga gcccgtagcc gtaaccggat acgctcatct gcaacggcgc
     6481 atcggtggtg atgcgaaaga cgctgccgga tgaaatgcgc cagagttgtt gcatctcacg
     6541 ggcactgctg cgtttgactg gaccacgttc gggctcgtca cggggcggaa acgccgacat
     6601 cagaaatgcg acgtagcgag cgagatcgtg cgtattggtc caaaggccgc ccatcgcgcc
     6661 aaacgagccg tgcgccagga tgggttcagc tttccagttg ttatcctcgc gacggtagcc
     6721 gagggcaatc tggtcgcgcg gcactcctga catttcgtag gtgctggagg tcatgcctag
     6781 cgggcgcagg atgtggtcgc gaacatagcc ggcgtaaggt ttaccggatg ctttggcgat
     6841 gacttgaccg agaatcgcaa agccgtagtt tgaatattcg aaggcggtgc caggcgcggt
     6901 ggagaacggt atgcctgatt tcatccaggc gcgcatggtt tcgtctgatt gggccaactg
     6961 gcga
//
LOCUS       DASTEY010000187         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1026041, whole genome shotgun sequence.
ACCESSION   DASTEY010000187 DASTEY010000000
VERSION     DASTEY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026041"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /gene="dusB"
                     /locus_tag="VFO72_03350"
     CDS             complement(<1..1006)
                     /gene="dusB"
                     /locus_tag="VFO72_03350"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET9478354.1"
                     /translation="MTTEIKPFKIRDVEINPPLVLSPMAGVTDVSFRRLLKRRGGVGL
                     SVSEFISVEGLTRNNPKSKRQMRFYEDERPFAVQIFGGQPERMCMAAEMAQEVGADIL
                     DVNCGCPAPKVVKHGGGSGLLKDHCRLEQILKAIKKTITIPLTVKIRAGFYDHTINAV
                     DTAKLAEDCGVEHIALHGRTKEQGYRGLANWDLVRQVKEVVRVPVSGSGDVTTIEGAF
                     AKFRETGCDGVLIGRGAMANPWIFRQIEDAMDGRPIFEPTLADKRAILHEYFDMLRED
                     MPETPAINRMKQLAGQFTRGLQGGALFRTSIYHSHTVEEVLDRIEEYFEAIESGRPYY
                     G"
     gene            complement(1003..1530)
                     /locus_tag="VFO72_03355"
     CDS             complement(1003..1530)
                     /locus_tag="VFO72_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9478355.1"
                     /translation="MTLIISFLICVVCANPWQNPPIEKPRIVVYKNARKLELYSDKKL
                     LRTYRVGLGFSPVQDKQREGDGATPEGDFYVFVKNNKSAYYLSLGVSYPNPEDAARGL
                     RDGLITKVQHDAIVEAHRKKTAPPQYTKLGGLIYIHGHGAKSDWTWGCVALENEDIKE
                     LYESVSVGTPVTIKP"
     gene            complement(1554..>2445)
                     /locus_tag="VFO72_03360"
     CDS             complement(1554..>2445)
                     /locus_tag="VFO72_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9478356.1"
                     /translation="QEEVAKRQSELIATLINEKLLLQKGKELELANDVEAEVNRRMLS
                     IAEGQGITTIEKLYEAMRQSGLNPEDVRRTMRTEMMKQAVIQQEVDRRVYLGFTPDEV
                     KKYFDAHPDKFRKPESIKLSEIWLSTTGKDEAAVKARALELIAQIRAGADFAAIAAAN
                     SEREKNGQRTAPQDKGYVGEFDVPNLREDLVTALKDVKAGGIAEPIRLGDGYQIIRVD
                     ARTPGGATPTFNDGRVREAMLMERQEKERDTYLQNLRNEAFIKVTEAYQAGVAPLLKL
                     RGPVAAKGQDKDDKKKSKKP"
BASE COUNT          492 a          764 c          597 g          592 t
ORIGIN      
        1 cgccgtaata cggccgcccc gattcgatcg cttcaaagta ttcctcgatg cgatcgagca
       61 cttcttcaac cgtgtgagag tgataaatcg aagtccgaaa taacgctccc ccctgcaaac
      121 cgcgcgtaaa ctgacccgcc aactgcttca tgcgattgat cgcaggggtt tcgggcatgt
      181 cttcgcgcag catgtcgaag tactcgtgca ggatcgcgcg cttgtcggct aacgtcggct
      241 caaagatcgg tcgcccgtcc atcgcgtctt cgatctgtcg aaagatccac ggattcgcca
      301 tcgcgccgcg tccaatcaaa acgccgtcac agccagtctc gcgaaacttc gcaaacgcac
      361 cctcgatcgt cgtgacatca ccactgcctg acaccggcac acgcacaact tccttcactt
      421 gccgcaccag gtcccaattc gcgagaccgc gatatccctg ttctttcgtg cgaccgtgca
      481 gcgcgatatg ctccactcca caatcttccg ccagcttcgc cgtatcgacc gcatttatcg
      541 tgtgatcgta aaagcccgca cgaatcttta cggtcagcgg gatcgtgatc gttttcttga
      601 tcgctttgag aatctgctcg agccggcagt ggtccttcag cagtccggat ccaccaccgt
      661 gcttcacgac cttcggcgcg ggacatccgc aattcacatc gagaatatcg gcgcctacct
      721 cttgcgccat ctccgccgcc atgcacatgc gctccggctg accgccaaag atctgcaccg
      781 caaacggccg ctcatcctcg taaaaccgca tctgccgttt cgacttcgga ttgtttcgcg
      841 tcaacccttc aactgaaatg aactccgaga cagacagacc aacaccgcca cgcctcttca
      901 acaggcgtcg aaacgagacg tcagtcacgc ccgccatcgg cgaaagaacc aatggcggat
      961 tgatctcgac atcgcgaatc ttgaatggct tgatctccgt cgtcatggtt ttatcgtaac
     1021 cggagttcct acagaaactg attcgtacaa ctccttaatg tcctcattct ccaacgcgac
     1081 gcagccccat gtccaatcac tcttagcgcc gtgaccatga atgtagatca gaccgccgag
     1141 tttggtgtac tgcggaggcg ccgtcttttt acggtgcgcc tcgacaatcg catcgtgttg
     1201 caccttcgtg ataagcccgt cacgcagtcc gcgcgctgcg tcttccgggt tgggatagct
     1261 gacgccgagc gacagatagt aagcgctctt attgttcttg acgaagacgt aaaaatcgcc
     1321 ttccggcgtc gcgccgtcac cctcgcgctg cttatcctgg acaggactaa atccaagccc
     1381 gacgcgatac gtgcgcagca acttcttgtc ggaatacaac tcgagttttc tggcgttttt
     1441 gtaaacgact atgcggggct tttcgatagg aggattctgc cacggattag cacagacgac
     1501 acagataaga aatgagatta tcagtgtcat ccgtgtaatc gtggctaaat tcgttagggc
     1561 ttcttggact tcttcttgtc gtccttgtcc tggcccttgg ctgcgactgg ccctcggagt
     1621 ttcaggagcg gcgcgacgcc ggcctggtaa gcctcggtca ctttaatgaa ggcttcgttg
     1681 cgcaggttct gtagataggt gtcgcgttcc ttttcctggc gctccatcag catcgcttca
     1741 cgcacacgac cgtcgttgaa agtcggagtc gctcccccgg gtgtgcgcgc atcgacacga
     1801 ataatctggt agccgtcgcc cagccggatc ggctcagcta tgccaccggc tttgacgtcc
     1861 ttcaacgcag tcaccagatc ctcgcgcaga ttcggaacgt caaactcacc gacgtaacct
     1921 ttgtcctgcg gcgccgtgcg ttgaccattc ttctcgcgct cggaattggc agcagcaatc
     1981 gcagcgaaat cagcacctgc gcgaatctgc gcgatcagtt ccagcgctct tgctttgacc
     2041 gccgcctcat ctttgcccgt cgtggacaac cagatctcgg acagcttgat gctctcgggc
     2101 ttgcgaaact tatcgggatg ggcatcgaag tacttcttaa cttcgtcggg agtgaaccca
     2161 agataaacgc gccgatctac ttcctgttgg atcaccgcct gtttcatcat ttcagttcgc
     2221 atcgtgcggc gcacatcttc aggatttagc ccgctctgtc gcatcgcttc ataaagcttt
     2281 tcaatcgtag tgatgccctg gccttcagct atgctcaaca tacggcggtt cacttccgct
     2341 tcgacgtcgt tggcgagctc aagctccttg cctttttgca gcagcagttt ttcgttgatc
     2401 agcgtggcga tcagttctga ctggcgcttc gctacttctt cctgc
//
LOCUS       DASTEY010000188         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1059181, whole genome shotgun sequence.
ACCESSION   DASTEY010000188 DASTEY010000000
VERSION     DASTEY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059181"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFO72_03365"
     CDS             complement(<1..229)
                     /locus_tag="VFO72_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478357.1"
                     /translation="MAARKEQSEPAAKVSVNMEELRELITLLRENGLAELELEREDFR
                     VRLRREGVFVQGDQGSAFTLTETLRNPLITDP"
     gene            complement(349..834)
                     /gene="rplQ"
                     /locus_tag="VFO72_03370"
                     /pseudo
     CDS             complement(349..834)
                     /gene="rplQ"
                     /locus_tag="VFO72_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(841..1896)
                     /locus_tag="VFO72_03375"
     CDS             complement(841..1896)
                     /locus_tag="VFO72_03375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9478358.1"
                     /translation="MAMDQNNLWQGFQMPRRLAADAETLTDRYGRFSAQPFERGFGTT
                     VGNSLRRALLSSIEGAAITAVKIEGVEHEFSSIKGVVEDATDVILNLKQVPFKLHSSE
                     SKTLRINKEEAGEVTAADIEADADVEILDPTVHIATVSEGGSLRVEMRLKRGRGYVSA
                     DRNFDEDLSLGYIPIDSVHTPVKKVNYNVEAARLGQNTEFDKLTIEVWTDGSVKPEDS
                     IGLAAKLIKDHMSIFINFEEDTDDYAYTKMERPPLPRNDQLDRSVDELELSVRSYNCL
                     KNADIRTIRDLVQRSEREMLATKNFGKKSLNEIKEILNGMGLDFGMEFDEQGNPIPGT
                     GGRDGADLSDFEGDEEQ"
     gene            complement(1915..2553)
                     /gene="rpsD"
                     /locus_tag="VFO72_03380"
     CDS             complement(1915..2553)
                     /gene="rpsD"
                     /locus_tag="VFO72_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9478359.1"
                     /translation="MARYRDAVCRLCRREGAKLFLKGDRCYKPACPIEKRGTQPPGQH
                     GNSLRRGKALVGYGQQLREKQKVKRIYFILEGQFRNYFERAARMKGITGENLLFLLER
                     RLDNAVYRAGFSTSRRQARQLVNHGHILVNDRKVDIPSYQVKPGDVVSVREPSRKNIH
                     IESAWQTASGRGRPSWITADGGDMGARVISLPVRDDIDRLINEQLIVELYSK"
     gene            complement(2794..3192)
                     /gene="rpsK"
                     /locus_tag="VFO72_03385"
     CDS             complement(2794..3192)
                     /gene="rpsK"
                     /locus_tag="VFO72_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9478360.1"
                     /translation="MAKAGGKKKVFRKKEKKNVPNGIVHIAASFNNTMISITDLEGNL
                     IAQSSAGARGFRGSRKGTPFAAQQAAFEAAKKAHEAGMHQCEVRVKGPGGGRESAIRA
                     IANAGIRVTTIRDTTPIPHNGCRPPKRRRV"
BASE COUNT          652 a          969 c          868 g          787 t
ORIGIN      
        1 ccggatccgt tatcagcgga ttccgcaacg tctcagtgag cgtgaaagcg gatccctgat
       61 caccctgcac gaagacaccc tcacgacgca accgcacacg aaagtcttca cgttcaagct
      121 caagctcggc gagcccgttc tcacgcagca acgtgatcag ctcgcgcagc tcttccatgt
      181 tgacggaaac tttcgccgcg ggttcgctct gttctttgcg tgcagccatt cgtctccagc
      241 cgccacagat tactcagact tatctgtgct ttctgtgtct tctgcgggtt cgctcttagc
      301 tgctttcttt gaggccttct tcttcgaact cgcagccttg gcccgtttct tggcggctac
      361 ggcggcctca atttcacgcg ggttatcgac gagctcgatg atcgcgagtt ccgccgcgtc
      421 gccatcgcga tggccgagtt tcaagatgcg cgtgtacccg cctggccgat ccttgtaacg
      481 ctcgccgagc tcgttgaaaa gacggcgcag tgcggcaaca cccgcggtgc gtcccggccg
      541 aggttggcct cgcttgccgg ccagctgtgc gactgccatg ttaccagcgt ggaagaaacc
      601 ggcggcctga cgccgcagat ggagtgcctg agcgtcgtcg tccgacagat tgcgcgcgcg
      661 gcgcgaaagt gtgatagcgc gctcgacgaa aggccgtagc tctttagcct ttggcaaagt
      721 ggtgacgatg cgctcctcac gggagttgat caatgaagtc gccagattgc gcaacatcga
      781 agtgcgatgt tcgctggtcc ggcccagttt gcgatgtgcc tttaagtgtc gcatgacgga
      841 ttactgttcc tcgtcacctt caaaatccga aagatcggcg ccgtcgcgac cgccagtgcc
      901 gggaatcgga ttaccttgtt cgtcaaactc catcccgaag tcgagaccca tgccgttgag
      961 gatctccttg atctcattga gcgacttctt gccgaaattc ttggtggcaa gcatctcgcg
     1021 ttcagaacgc tggaccagat cgcggatggt gcgaatgtcg gcgttcttca aacagttgta
     1081 agaccgcact gaaagctcga gctcatcgac cgaacggtcc aactggtcgt tacgcggaag
     1141 gggcggacgc tccatcttag tgtaggcgta gtcgtcggtg tcttcctcga agttgatgaa
     1201 gatcgacatg tggtccttga tcaacttcgc cgccaaacca atcgagtctt ccggcttcac
     1261 cgaaccgtca gtccagactt cgatagtcag cttgtcaaac tccgtgtttt ggcccagacg
     1321 tgccgcctcg acgttgtaat taaccttctt gacgggcgta tggaccgaat cgatcgggat
     1381 gtaaccaagc gacaagtctt catcgaaatt tcgatccgcg gagacgtacc cgcggccacg
     1441 tttcaggcgc atctcgacgc gcaacgatcc accttcgctg acagtagcga tgtggacggt
     1501 cggatcgaga atctcgacgt cggcatcagc ttcgatatca gccgcagtga cttcgcccgc
     1561 ctcttccttg ttgatacgga gagttttcga ctcgctgctg tgaagtttga agggcacctg
     1621 tttcaaattc aggataacgt cggtggcatc ttccaccacg cccttgattg aggaaaattc
     1681 gtgctccacg ccttcgatct tcactgccgt aatggcggcg ccttcgatcg acgagagcaa
     1741 cgcacgccga agcgaattgc cgacagtcgt accgaagccg cgttcaaacg gctgggccga
     1801 gaatcggccg tagcgatccg tcagcgtctc tgcatctgcc gccagccggc gcggcatttg
     1861 aaatccctgc caaagattat tttgatccat agccatagcg ctcagtccct aaaattactt
     1921 gctataaagt tccacaatta gctgttcgtt gatcaagcga tcgatgtcgt cgcgtacggg
     1981 caaactgatg acacgcgcgc ccatgtcgcc gccgtcagcc gtgatccagg acgggcggcc
     2041 gcggccggat gctgtttgcc atgcgctttc gatgtgaatg ttttttctgc tgggttcccg
     2101 cacactgacc acgtcgccag gcttcacctg ataagaagga atgtcgactt tgcgatcgtt
     2161 caccaggatg tgaccgtgat tgaccaactg tctggcctgg cggcgagaag tggaaaaacc
     2221 agcgcgatag acagcattgt ccagtcgccg ctccaacagg aacaggaggt tctcgccggt
     2281 gatccccttc atgcgagcag cgcgttcgaa gtagttacga aactgccctt cgagaatgaa
     2341 gtagatacgc ttgaccttct gtttctcgcg caactgctgg ccgtagccga ccagtgcctt
     2401 gccgcgtcgc agcgagttgc catgctgccc gggcggttga gtaccgcgtt tctcaatcgg
     2461 gcaagcgggt ttgtaacaac gatcgccttt caaaaagagc tttgcgcctt cgcgtcgaca
     2521 aaggcgacac actgcatctc tatacctagc caactatctg cctccagtct aaataagacg
     2581 gaaaagttta caggtcaggt cattgccaat tttcgattgc cgattgccga tttgtcgcgg
     2641 cgtgcaaccg ttatgacgat accggaccct tcatcctttg ggttatgtca ttgtcgattt
     2701 tcgattttcg attgccaatt tcacgccaac tgcgaactcg ccgtcactct gcaaatcgac
     2761 aatcggcaat cggctatcgg caatcaaaaa tgcctacact ctacgccgct ttggcggcct
     2821 gcaaccgttg tgcggaatgg gcgtcgtatc gcggatcgtt gtgacgcgaa taccggcgtt
     2881 cgcaatcgcg cgaatcgccg actctcgacc accgcccggg cctttcacgc gcacctcaca
     2941 ctggtgcatt cccgcttcgt gcgccttctt ggccgcttcg aaagccgcct gctgcgctgc
     3001 gaagggcgtt cccttgcgcg acccgcgaaa accacgcgcg cctgccgatg attgcgctat
     3061 caggtttcct tccaggtcgg taatcgagat catcgtgttg ttgaacgacg ccgcgatatg
     3121 aacgatgcca ttgggaacgt tcttcttctc tttcttgcga aagaccttct tctttccacc
     3181 tgcttttgcc ataacttttg tgctttgctc tttgtgcttt gtgcttgtgc tctgctagtc
     3241 gcgcacaatg cgtcaactac aaagctctaa gcacaa
//
LOCUS       DASTEY010000189         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1636003, whole genome shotgun sequence.
ACCESSION   DASTEY010000189 DASTEY010000000
VERSION     DASTEY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636003"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFO72_03390"
     CDS             <1..504
                     /locus_tag="VFO72_03390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9478361.1"
                     /translation="WGEIGLDFHYDNSPREVQMEVFRRQLRAARECNVPVIVHTREAE
                     SETIQILESEYAGAERGGVFHCFSGSKTLATRALELGFMISFSGIVTFRKAEDLREIA
                     KRVPLESLLIETDCPYLAPVPHRGRRNEPAYVVDVARCIAELRGLEPEEVARITTENF
                     KRFLSHR"
     gene            623..3094
                     /locus_tag="VFO72_03395"
     CDS             623..3094
                     /locus_tag="VFO72_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9478362.1"
                     /translation="MKQLFLVFLLLFPVSVFGQHGHAPAKEPASTALAAGLGDINHPV
                     STTNADAQKFFNQGLAYIYAFNHDEAVRSFKQATVLDPQLAMGYWGMALALGSNYNVQ
                     ADGPSLLTAYNNLQKAIELAPKASEHERAYINALAKRYSADLNADKNKLALDYKNAMG
                     ELTKRYPDDLDAATLYAESIMNLRPWKLWSLDGKPAEDTLEIVAVLEGVLRRNPNHTG
                     ANHYYIHAVEASSNAERGLPSAARLGKIAPKAGHLVHMPSHIYIRTGDYGDAAKSNLD
                     AIAADREYITKTGAQGLYTMMYYNHNIHFLASASALKGRYADSIKAARELETNIAPHL
                     KAMPMLEMFRPYPIVAQVRFGRWDDLLKEPKPDEALKVTTGYWHYARGSAYAAKNDMA
                     NADAELKALQAVIKAIPADAPLGNSLAAGVLKVAELTLAGRIAFARGDKQAAFNFLNQ
                     AAAAEDVTGYNEPADWDLPVREVLGGLLLSTGDNAAAEKAFRDELKRHPRNGRALFGL
                     AESLKRQGNNTSAQMVWREFEEAWKDADTKLTVDGLSGMTTQTESGATASNTSSVQFS
                     SVLLKTGVRLRYAYQGDPNGIPVIMLHGYTDSWFSFSPVLPFLDRKYRVYSLDQRGHG
                     ESDRPVGGYAMQQFAADVVAFMDAMNIKQATIVGHSMGSFVAQHVAVEAPERVKRLVL
                     VATATTVHTNDLARELQRSVNGLSDPVSEKFVHDFQVSTIFRQVPNEFLHNVVKESMK
                     LPAHVWREVMAEMISPDAGVPLKKIKTPTLILWGDKDFFPRSEQDSLVSALPDAMLKV
                     YKDTGHALHWERPEKFATDLQAFIN"
     gene            3102..3662
                     /locus_tag="VFO72_03400"
     CDS             3102..3662
                     /locus_tag="VFO72_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9478363.1"
                     /translation="MNGGELDTLIQNARTIADDARSSFGDLIAEQLNWKPSPERWSVA
                     QCFDHLIASNKGYLPIIESVRGGTKKKTFFERLPVLPGLAGKLLIKSLDPASTRKLKA
                     PKSFEPAQSNISASVIDDFVAQQEKVVEGMKSTSHLDLERIVITSPAASFVTYSLMDA
                     YRIIVVHERRHFQQAQRVTAEPGFPA"
     gene            complement(3664..>3844)
                     /locus_tag="VFO72_03405"
     CDS             complement(3664..>3844)
                     /locus_tag="VFO72_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478364.1"
                     /translation="DRQRARELYGRIQEIVSRDVPVFPLWYQSNIVIARKNVGNIQVN
                     ASGDWGFVKNLTVNE"
BASE COUNT          867 a         1078 c         1104 g          795 t
ORIGIN      
        1 tggggcgaaa ttggcctgga ttttcactac gacaactcac cgcgcgaggt acaaatggaa
       61 gttttcagac gtcaactgcg tgccgcgcgc gaatgcaacg tgccggtcat cgttcacacg
      121 cgcgaggctg aaagcgaaac gattcaaatc ctcgagagcg agtacgccgg agccgaacgc
      181 ggcggtgttt ttcactgctt cagcggcagc aaaacactcg caacccgcgc gctggagctc
      241 ggtttcatga tctccttttc aggcatcgtt acgtttcgca aagcggaaga ccttcgagaa
      301 atcgcaaaac gcgtcccact cgaaagtctc ctgatcgaaa ctgattgccc ctacctcgct
      361 cccgttccgc atcgcggaag acgcaacgaa cccgcttacg tcgtggatgt cgcgcgttgc
      421 atcgccgaac tccgcggtct cgagcctgaa gaagtcgccc ggataaccac agagaatttc
      481 aaacgcttcc tgagccacag atgaacacgg agggacacag acagagagaa ttaactcagt
      541 ccgtgtttgt ccgtgttcat ctgtggcccg cactggtccg tttccgaccg attttacgcg
      601 tctatgaatg aggagatttc cgatgaaaca actgttcctt gtgtttttgc tgcttttccc
      661 cgtttcggtg tttggtcagc acggtcatgc tccggccaaa gagccggcgt ccactgctct
      721 tgcggctgga ctgggcgata tcaaccaccc ggtttccacc accaacgccg acgcgcagaa
      781 gtttttcaat cagggactcg cgtatatcta cgcgttcaat cacgacgagg cggtccgttc
      841 gttcaagcaa gcaaccgtac tcgacccgca actcgcaatg ggttactggg gtatggcgct
      901 cgcacttggt tcgaactaca acgtgcaggc tgatggacca tcgctgctga ccgcttacaa
      961 caatctacag aaggcgatcg aactcgctcc caaagcttcc gaacacgaac gcgcgtacat
     1021 caacgcgctc gcgaagcgtt attcagccga tctgaacgcc gacaaaaaca aactcgcgct
     1081 cgattacaag aacgcgatgg gcgaattgac gaaacgctat cccgacgacc tggacgccgc
     1141 cacgctttac gctgaaagca taatgaacct gcgcccgtgg aagctgtgga gtctcgacgg
     1201 caagcctgct gaagacacgc tcgaaatcgt tgcggtgctc gaaggcgtgc tgcgccgcaa
     1261 cccgaatcat acgggagcca atcactatta cattcacgcc gttgaagctt ccagcaacgc
     1321 cgagcgcgga ttaccgagcg ctgcgcgtct cggaaagatt gcgccgaagg ccggacacct
     1381 ggtccacatg ccttcgcaca tctatatccg caccggcgac tacggtgatg cggcgaagtc
     1441 gaacctcgat gcgattgccg cggaccgcga gtacatcacg aagactggcg cacagggcct
     1501 gtacacgatg atgtactaca accacaacat tcacttcctc gcttcggcga gtgcgttgaa
     1561 gggacgttat gcggattcaa tcaaggcggc acgcgagctc gagaccaata tagcgccgca
     1621 cctgaaagcg atgccgatgc tcgagatgtt caggccgtat ccgatcgtcg cgcaggtgcg
     1681 cttcggtcgt tgggacgatc tcctgaaaga accgaagcct gacgaagcgt tgaaggtcac
     1741 gacgggctac tggcactacg cgcgtggcag cgcttatgcc gcgaagaacg acatggccaa
     1801 tgccgacgct gagttgaagg cgttgcaggc agtaatcaaa gccattcctg ctgacgcgcc
     1861 gctgggcaac agtctcgctg ccggcgtgtt gaaggttgcg gagctaacgc tggccggaag
     1921 gattgccttc gcgcgcggcg acaagcaggc cgcgttcaat ttcctgaacc aggcagccgc
     1981 tgccgaagac gtaaccggtt ataacgaacc tgcggattgg gatttgccgg ttcgcgaagt
     2041 gctcggtggc ctgttgctca gcactggcga taacgccgcg gccgaaaagg cgtttcgtga
     2101 cgaactaaag aggcacccgc gcaatggccg cgcgttattc ggactcgctg agagtttgaa
     2161 gcggcagggc aataatacgt cggcgcagat ggtctggcgc gagtttgaag aggcgtggaa
     2221 agacgcggat acaaaattaa cggttgacgg tctgagtggt atgacgacac agaccgaaag
     2281 cggcgccacg gcgagcaaca cgtcctccgt gcagttttcg agtgtgttgc tcaagactgg
     2341 tgttcgtttg cgttatgcct accagggcga tccgaacggt attccggtga tcatgctgca
     2401 cggttatacc gattcatggt tttcgttcag ccccgtgctg ccgttcctgg atcggaagta
     2461 tcgcgtctac agcctggatc agcgcggcca cggtgagtca gatcggccgg ttggcggcta
     2521 cgcgatgcag cagttcgccg ctgacgtggt ggccttcatg gacgcaatga acatcaagca
     2581 ggccacgatt gtcggccact cgatgggaag tttcgtggcg cagcacgtgg ccgtggaggc
     2641 gccggagcga gtcaagcgac tcgtcctcgt agctacagcc acgactgtgc acaccaacga
     2701 tctcgcacgt gaactgcaac gctcggtcaa tggactatct gatcctgtgt cggagaaatt
     2761 cgtgcacgac ttccaggtga gcacgatttt taggcaggtg ccgaacgagt ttctgcacaa
     2821 cgtggttaaa gaaagtatga agttgccggc gcacgtgtgg cgcgaggtga tggctgagat
     2881 gatctccccc gatgcgggtg tgccgttgaa gaagatcaag acgccgacac tgatcctttg
     2941 gggcgacaaa gacttcttcc cgcgatcaga acaggactcg ctggtgtccg cgttgcccga
     3001 cgcgatgctg aaggtctaca aagacaccgg acacgcgttg cactgggaac ggccggagaa
     3061 gtttgccacg gatcttcagg cgttcataaa ctagctttgc gatgaacggt ggcgagttag
     3121 atacactgat ccagaacgcc aggacgatcg cggacgatgc gcgatcgtct tttggcgact
     3181 tgattgccga acaattgaac tggaaaccgt ctcccgagcg ttggagcgtt gcccagtgtt
     3241 tcgatcatct gatcgcgtcc aacaaaggtt acttgccgat tattgaaagt gttcgcggtg
     3301 ggactaaaaa gaagacgttt ttcgagcgtc tccccgtatt gccgggtttg gcgggaaagc
     3361 tgttgattaa gtcgctggac ccggcatcca ctcgcaagct aaaggcgccg aagagtttcg
     3421 aaccggcgca gagcaacatc agcgcgagcg ttatcgacga ctttgtcgcg cagcaggaga
     3481 aggtggtcga aggcatgaag tcgacgagtc atctcgatct cgaacggatc gtgatcacat
     3541 cgccggctgc gtcttttgtc acttacagtt tgatggacgc ctaccgcatc atcgtggtcc
     3601 acgaacggcg gcattttcag caggcacaga gagtaacggc tgaaccggga tttcccgcgt
     3661 gatttattcg ttgacggtca ggttcttcac aaagccccaa tcgccactcg catttacctg
     3721 aatgtttccg acgttctttc gcgcgatgac aatatttgac tggtaccaca acggaaacac
     3781 aggcacgtcg cggctgacaa tctcctgaat cctgccgtac aactcgcgcg ccctctgccg
     3841 gtcg
//
LOCUS       DASTEY010000190         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_432802, whole genome shotgun sequence.
ACCESSION   DASTEY010000190 DASTEY010000000
VERSION     DASTEY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432802"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFO72_03410"
     CDS             complement(<1..707)
                     /locus_tag="VFO72_03410"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9478365.1"
                     /translation="MLKRVLFFAYGVFCYLVFLGTFLYAIGFIGNFGVPTTLDGPATG
                     PFLVSFAIDAALLTLFAVQHSVMARKWFKQWWTTIVPKPIERSTYVLFSSLALILLFA
                     FWQPLGGVIWSVEDPAGRFVLRALFAFGWGLVLISTFLINHFDLFGLRQVWRYLLGQT
                     AEGLRFVTPGPYRLVRHPLYAGWLFAFWMTPLMTSAHLLFSIATTAYILVAIQLEERD
                     LVREHGETYEEYRRSVP"
     gene            843..1919
                     /locus_tag="VFO72_03415"
     CDS             843..1919
                     /locus_tag="VFO72_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9478366.1"
                     /translation="MSKRATQRIRYLRTRDGVQVAWAEAGTGPTLVKAANWMTHLEYE
                     WESPVWRHWIRFFSDHFRFIRHDERGCGMTDWNVGDLSFERWVEDLEAVVVVVHPQEP
                     FALLGISQGAATCIAYAVKHPERVSHLVLYGAYARGTYQRGDADKERLYRALIEMTRL
                     GWGKDNPVFRQVFTSRFIPGASDEQIGWFNDLCRKTTSPEIAARLLETRALINVVDLL
                     GQVQAPTLVLHSRDDDVIPIGEGHILAAGIPRAQFVELDSKNHILLETEAAWKRFCEE
                     VLDFMDVKGEVRGEDPAFASLSPREREVLALITEGLGNAEIAERLSISEKTVRNHVSN
                     LFDKLGVWTRAQAMVFAHDHGFRE"
     gene            1926..2525
                     /locus_tag="VFO72_03420"
     CDS             1926..2525
                     /locus_tag="VFO72_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478367.1"
                     /translation="MSSKFALLTIAIAFVLSSFTSAGRAQSPNLLQNPNADLGNSSWR
                     VFGNATIETWQENACFVVRDGGYFMQDVELPDDAVGEYVLFLGMGASERINADGAITG
                     LPYLYGYMMPQSGRSNSDTVLEYLQGQRMRAQTTGENEWVSMWGIFKVPKGTKRVRFF
                     LNQALRAGVPHNGSAARFDELGLYLFATKEEAEAFVAVR"
     gene            complement(2847..>3357)
                     /locus_tag="VFO72_03425"
     CDS             complement(2847..>3357)
                     /locus_tag="VFO72_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478368.1"
                     /translation="NMSGKSTLLRTVGVNVVLALAGAPVRVRSLRLSPLQVGASIRIQ
                     DSLQAGASRFYAEITRLRQIVELTNGERPVLFLLDEILHGTNSHDRQIGAEKVVRGLI
                     ERGAIGLITTHDLALARIADELGRRAANVHFEDHLEDGEMRFDYLLRPGVVQRSNALE
                     LMRSVGLEV"
BASE COUNT          777 a          918 c          962 g          700 t
ORIGIN      
        1 atcggcacgg atcttcgata ctcttcatac gtctcaccgt gctcgcgaac gagatcccgc
       61 tcttccaatt gaatcgctac caggatgtaa gccgttgtgg cgatcgaaaa gagcagatgc
      121 gctgacgtca tcaacggcgt catccagaac gcgaaaagcc agccggcgta gagtggatgt
      181 cgcaccagac gatacggacc gggcgtaaca aaccgcaagc cttcagcggt ttggcccagc
      241 aagtatcgcc acacctggcg cagtccgaaa agatcgaagt gattgatcag gaaggtagag
      301 atcaacacta atccccagcc aaaagcaaac aacgcgcgca gcacgaagcg tccggccggg
      361 tcttcgacag accagatcac accacccaat ggctgccaga aggcaaacaa cagaatcaga
      421 gcgaggcttg agaacagcac gtaagtagag cgctcaatcg gcttcggcac aatcgtcgtc
      481 caccattgct tgaaccactt gcgcgccatc acgctgtgtt gtacggcgaa tagcgtgagc
      541 aaagctgcgt cgatcgcgaa actgacgagg aacggtcccg tcgcaggtcc atccagtgtg
      601 gtcggaacac cgaagttgcc gatgaatcca atcgcgtaca gaaacgtacc caggaacacc
      661 aaataacaaa aaaccccgta cgcaaagaat agaactcgct tgagcattgt gtggcctccg
      721 tcgctgctgt ttcgccgcgc agcatcgcac gttcctgcgc tgttaggcta tgggagttta
      781 gtcccggttt ggatatactc cgatgccttc gcatgggact tttgtctcgg tattgatgtt
      841 cgatgagcaa gagggcaaca cagcgcattc gctatctccg cacgcgtgat ggcgtgcagg
      901 tcgcctgggc tgaggcgggc acgggcccga cactggttaa agctgcaaac tggatgaccc
      961 atctcgaata cgaatgggaa agtccggttt ggcggcactg gattcgtttc ttcagcgacc
     1021 acttccgctt cattcgtcat gacgaacgcg gctgcggcat gaccgactgg aacgtcggcg
     1081 acctttcgtt cgagcgttgg gtagaggatc tggaggccgt cgtcgtggtc gtgcatccgc
     1141 aggagccgtt cgctttgctc ggcatttctc aaggagcagc gacgtgtatc gcgtatgccg
     1201 taaagcatcc cgaacgcgtt tcgcacctcg tgctttacgg cgcctacgcg cgtggaacat
     1261 atcagcgtgg cgacgcggat aaggagcgtc tgtatcgcgc tcttatagaa atgactcgtc
     1321 tcggatgggg caaagacaat cccgtcttcc ggcaggtgtt cacttcgcgg tttattcccg
     1381 gcgccagcga cgaacaaatc ggctggttca acgatctctg tcgaaagaca acgtcacccg
     1441 agatcgcggc acgacttctc gagacgcgcg ctctgatcaa tgtggtcgac ctgctgggcc
     1501 aggtgcaggc gccgacattg gtcctgcatt cacgcgatga cgatgtgatt ccgattggtg
     1561 aaggacacat tctggcagcg ggaattccgc gcgctcaatt cgtcgagctt gactcgaaga
     1621 accacatcct gcttgaaact gaggcggcat ggaaacgttt ctgcgaggaa gtgttggatt
     1681 tcatggacgt gaaaggtgaa gttcgcggtg aagatccggc gtttgcttca ctgtcgccgc
     1741 gcgagcgaga agtgctggcg ctgattacgg aagggctagg caatgctgag atcgcggagc
     1801 gtctttcgat cagcgaaaag acggtgcgca atcacgtgtc gaatctgttt gacaagctgg
     1861 gcgtttggac gcgtgcgcag gcgatggtgt ttgcgcacga ccacggattt cgggagtaaa
     1921 aaccaatgag ttcaaagttt gcactgctga caattgcaat cgctttcgtt ctttcaagtt
     1981 tcacttcggc cggccgcgcg caatcgccaa acctgctgca aaatccaaat gcggatctgg
     2041 gcaacagttc ctggcgagtg tttggcaacg cgacgattga gacctggcaa gagaatgctt
     2101 gtttcgtcgt gcgcgacggt ggttatttca tgcaggacgt tgaacttcct gacgacgcag
     2161 ttggcgagta cgttctgttt ctcggaatgg gcgccagcga gcgcatcaac gctgatggcg
     2221 cgattactgg tctaccgtat ttgtacggtt acatgatgcc gcagagcggg cggtccaaca
     2281 gcgacaccgt gttggaatat cttcaaggtc aacgaatgag ggcgcagacg accggcgaga
     2341 acgaatgggt gagcatgtgg ggaatcttca aggtgccgaa aggaaccaaa cgagtacggt
     2401 tctttttgaa tcaggcttta cgtgcgggcg ttccgcacaa cggctcggca gcacgcttcg
     2461 acgagctcgg cttatacttg tttgctacga aagaagaggc tgaggctttt gttgccgtaa
     2521 ggtgaaaatg aaaagaacgg tacttaagcg ggttctactg gtgtttctgc tcaatggcaa
     2581 ccatcgacgg tggcgaaggc agcaaagcca tctccttctc gttccagcga gatgaagcct
     2641 ctcgaatgaa gtaaacctga ttttcctggg accgcaggcg tctcgcctgc ttgcagcgaa
     2701 gaaggccact gccgaatcca gcaggcgaga cgcctgcggt cccaggaaag agcacgaccc
     2761 aggcaaagag cacgacccag gcaagagcac gaccggctaa cttaaagcct ggcgccttcg
     2821 gcaagtcgtt tttcgtccga tcacgattac acctccagac caaccgaacg catcaattct
     2881 agcgcgttgc ttcgttgcac gacgccgggt cgcaggagat agtcgaagcg catctctccg
     2941 tcttcgaggt gatcttcgaa gtgaacgttg gctgcgcgtc gtccaagttc atcggcgatt
     3001 cgcgccaacg ccagatcgtg cgttgtgatc aaaccgatag cgccgcgctc tattagacca
     3061 cgcaccacct tctccgctcc gatctgccgg tcgtgtgagt tggttccgtg gaggatttcg
     3121 tcgagcagaa aaagcacggg acgttccccg ttcgtcaatt ccacaatctg ccgcaaccga
     3181 gtgatctctg cataaaagcg cgaggcgcct gcttgaagag aatcctggat tcgaatcgac
     3241 gctcccacct gaagcggcga caaacgcaaa ctcctgacgc gaacgggagc acccgcaagc
     3301 gccagcacta cattgacgcc gaccgtgcgc agcaaagtgc tcttccccga catgttc
//
LOCUS       DASTEY010000191         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1339152, whole genome shotgun sequence.
ACCESSION   DASTEY010000191 DASTEY010000000
VERSION     DASTEY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339152"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VFO72_03430"
     CDS             complement(<1..1256)
                     /locus_tag="VFO72_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478369.1"
                     /translation="MGSFDKFLTRVFGSSNQRYLKSIQPLVEKINALEPSVQKLSDDE
                     LRARTAAFKEQVQRAVGDVTEKEERKRRERAVLDEILPEAFAIVREASVRTTSMRHFD
                     VQLIGGIVLHQGKIAEMRTGEGKTLVATLPSYLNALTGRGGVHVITVNDYLASRDAEW
                     MGQIHRFLGLEVGCIQNDMDDFERQAAYAADITYGTNNEFGFDYLRDNMKFDLATCVQ
                     RGHYFAIVDEVDSILIDEARTPLIISGPSDEATDKYKNADAIIPHLKKGEDIDGKKTG
                     DYVVDEKAHTAVLTEEGVEKAERLLGVGNLYDPSNMELLHCVEQALKAHTLYKLDHQY
                     VVQDGEVIIVDDFTGRLMKGRRWSDGLHQAVEAKEGVEIEKENQTLATITLQNYFRLY
                     EKLSGMTGTAETEAAEFASTYKLDVIV"
     gene            complement(1300..2421)
                     /locus_tag="VFO72_03435"
     CDS             complement(1300..2421)
                     /locus_tag="VFO72_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478370.1"
                     /translation="MTELFENFEVNRDSGWAVLLRLLGASLVLHLVLVWVLVSVPVFR
                     DTLNIAALVASTKFVDKDYVATQIGDDVEIVQLPSEKFRYPEGYFVLEAQIDGEVIAQ
                     APAAADPSAPQIISQWNPSKNVDQQASPSPSPEASASPTASPAASPTVSSAASPSPAI
                     AQASPSPGATGTPLSVEEAQKKLEETAAQNNVPLPDENEINKKVLKDFAAYANNLKNE
                     GKLDLNKPFEVVIEAEMDEKGKLQNPRFTKKAGDENLIDLFARMVAALNDSGFLTYLQ
                     PISKDNPGAKVVITIKQGETEVIASVESETTSTARAESLANALNNLLFFGAGSRAGKD
                     EEVLMKNTSATPDGKKVVVNFSMPRQSVVDMIKKQLEPG"
     gene            2530..3348
                     /locus_tag="VFO72_03440"
     CDS             2530..3348
                     /locus_tag="VFO72_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9478371.1"
                     /translation="MRDKGRRRWDRRSTHRSKRPDRNAPAPHQPERRIPQSRDVKKLL
                     HGIGTPPPAPFKPDQFQLEALAMLEYEDVLVTAPTGSGKTWIAREEIRRLLNDGKRAW
                     YTTPLKALTNSKYQEFIDEFGAQRVGILTGDRKENSDAPLIVGTTEIYRNQLFDSLRT
                     GSEVYSDLVVLDEAHYLADEERGHVWEEAIILTPPRIRLLLLSATIGNAGDFASWLEE
                     VRGVRCGVITGPGKRPVPLRSAVLLPDRRLLPLIEESGRVNQQIAQLIHETANA"
BASE COUNT          696 a          938 c          927 g          790 t
ORIGIN      
        1 acgataacgt ccagtttgta agtcgaagca aactccgcgg cttccgtttc agcggtaccg
       61 gtcataccgg acagcttttc gtagagacgg aaatagtttt gcagcgtgat cgtcgcgagc
      121 gtttggttct ccttctcgat ctccacgcct tctttggctt caactgcctg atgcagaccg
      181 tctgaccagc ggcgaccttt catcaggcgt ccggtgaagt cgtcgacgat gatgacttcg
      241 ccgtcctgga ccacgtactg atgatcgagc ttgtaaagcg tgtgggcctt caatgcctgc
      301 tcgacgcagt gcagcagttc catgttcgaa ggatcgtaaa gattgccgac acctaacagc
      361 cgttcggctt tctcgacgcc ttcttcagtg agcactgccg tgtgcgcttt ttcatccaca
      421 acgtaatcgc cggtcttttt gccgtcgata tcttcgccct ttttgaggtg cgggatgatc
      481 gcatcggcgt tcttgtattt atcggtggcc tcgtcggaag gaccggaaat gatgagcggc
      541 gtacgcgctt cgtcgatcag gattgagtcg acttcgtcga cgatggcgaa gtagtgacca
      601 cgctgcacgc aggtggcgag gtcaaacttc atgttgtcgc gcaggtagtc gaagccgaat
      661 tcgttgttgg tgccgtacgt gatgtccgcg gcgtaggctg cctggcgttc gaagtcgtcc
      721 atgtcgttct gaatgcaacc gacttcgagg ccaagaaagc gatgaatctg gcccatccat
      781 tccgcgtcgc gcgaagcgag gtaatcgttc acagttatga cgtgaacgcc gccgcgcccg
      841 gtgagcgcat tcaaatacga aggcagcgtc gcgaccagcg ttttaccttc gccggttcgc
      901 atctcagcga tctttccctg gtgtagcacg atgccaccga tcaactgcac gtcgaagtgg
      961 cgcatgctgg tcgtgcgcac gctcgcttct cgaacgattg cgaaggcttc cggcagaatc
     1021 tcgtcgagga ctgctcgttc gcgtcgcttg cgttcttcct tctcagtaac gtcgccgacg
     1081 gcacgttgga cctgttcctt gaaagccgcg gtgcgcgcac gcaactcgtc gtctgaaagc
     1141 ttctggaccg acggttcgag tgcattgatc ttttcaacca gcggctggat ggacttcaga
     1201 taacgctggt tactgctgcc aaatactctc gttagaaatt tatcgaaact acccataaca
     1261 gtgttgaatt ttagtttagg tctgaaaaag gcgcaaagat tatccgggtt ctaactgctt
     1321 cttaatcatg tcaacgacgc tctgccgcgg cattgagaaa ttcactacga ctttcttccc
     1381 gtccggcgtc gcgctcgtat tcttcatcag cacttcttcg tctttaccgg ctcgcgagcc
     1441 ggcgccaaaa aagagcaggt tgttgagcgc gtttgccaac gactcagcgc gcgccgtaga
     1501 tgttgtctct gactcaaccg acgcgattac ctcggtctct ccctgtttga tcgtgatgac
     1561 aactttcgcg cccggattgt ctttgctgat cggttgcagg taggtcagaa aaccactgtc
     1621 gttcagtgcc gccaccattc tcgcaaacag atcgatgaga ttttcatcgc ccgccttttt
     1681 ggtgaaacgc ggattctgaa gtttgccctt ctcgtccatt tcagcttcga tcacaacttc
     1741 gaagggtttg ttcaagtcga gcttgccctc gttcttcagg ttgttcgcgt aagccgcgaa
     1801 atctttcaag actttcttgt tgatctcgtt ctcgtcaggc aaggggacat tgttctgagc
     1861 ggcagtttct tcgagtttct tctgcgcctc ttccacgctc agcggcgtgc cggtcgcgcc
     1921 tggcgacgga gacgcctgtg cgattgccgg cgatggcgaa gcggcagagc ttacagttgg
     1981 ggaagcggcc ggacttgctg ttggcgaagc gctcgcctcc ggactcggcg atggtgacgc
     2041 ttgttgatct acattcttag aggggttcca ctgcgaaatg atctgcggcg cagacgggtc
     2101 cgcggctgcc ggggcctgag cgatcacttc gccgtcgatt tgtgcctcga gaacaaaata
     2161 gccctcagga taacgaaact tttcgctcgg gagctgaacg atctccacgt catcaccgat
     2221 ctgcgtcgca acgtaatctt tatcaacaaa ctttgtgctc gcaacgaggg cggcgatatt
     2281 gagcgtgtcg cggaataccg gaacggaaac caggacccaa acgagcacga gatgaagcac
     2341 gagtgaggcg ccgaggagcc gcaagagcac tgcccacccg gagtcgcgat taacctcaaa
     2401 attttcgaac agctcagtca tccctaaatg aaacgtcgct tggcctcttt tgtttcagtg
     2461 cctttagacg ccgcgcgcgg tcgacgcgtt gtgagacaga ttgtaactcg cccggctaga
     2521 atgtgcgtca tgcgggacaa gggtcgaaga cgctgggatc ggcgatcaac acatcgttca
     2581 aagagacctg atcgaaacgc ccccgctccg caccagccgg aacgccgaat cccccaatct
     2641 cgagacgtta aaaaactgct ccacggaatc ggtacaccgc cacccgctcc attcaaaccc
     2701 gaccagtttc aactcgaagc actcgcaatg ctcgagtacg aagacgtgct agtgacggcg
     2761 ccgaccggca gcggcaagac ctggatcgct cgcgaggaga tccggcgtct gctcaacgac
     2821 ggaaagcgtg cgtggtacac aacgcccctt aaggcgctca cgaactctaa ataccaggag
     2881 ttcatcgacg agtttggcgc acagcgcgtc ggcattctga ccggagaccg caaggaaaac
     2941 tctgacgcac cgctgatcgt cggcaccacc gagatctacc gcaaccagct ttttgattca
     3001 ctacgaaccg gaagtgaggt ctattccgac ctcgtcgtgc tggacgaagc acactatctc
     3061 gccgacgaag aacgcggaca cgtttgggaa gaagcgatta ttttgacgcc gccgcgcatt
     3121 cgactgttgt tgttgtctgc aacgattggc aacgcaggcg atttcgcgag ctggttggag
     3181 gaagtgcgtg gtgtgcgctg cggagtgatt acaggtccgg gcaaacgtcc ggtgccgctg
     3241 cgttcggcgg tgttgcttcc ggatcggcga ctgttgccgt tgatcgagga gagtggaagg
     3301 gttaaccaac agatcgcaca gctaattcat gaaactgcga atgcctgaaa t
//
LOCUS       DASTEY010000192         4728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1355779, whole genome shotgun sequence.
ACCESSION   DASTEY010000192 DASTEY010000000
VERSION     DASTEY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355779"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFO72_03445"
     CDS             <1..335
                     /locus_tag="VFO72_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET9478372.1"
                     /translation="RGVWGWTLLAIIWCLAIAGVVIKALPRTRHSWISMVLYLVMGWL
                     AIVAIKPIMELVPVPGILLILAGGFAYTSGLAFFAASRLRYNHFIWHLFVIAGTTFHF
                     FAVLWYAA"
     gene            complement(947..3976)
                     /locus_tag="VFO72_03450"
     CDS             complement(947..3976)
                     /locus_tag="VFO72_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9478373.1"
                     /translation="MRTPVFRSFPRLQTFATSLILIVLFAPAIAAQTQSTTGTIEVVV
                     SDANGAVIPGASVETRNLDTNLTRTGTTDDNGRFVALALQPGNYSVTVSKQGFTTGVL
                     ERTQLTVGEAKNIPVTLTVSQVAERVTITDTPTVDTLKTQVSSTINETAVSTTPILGR
                     KFEDLLTLTPGVSITQGPDGDEINFAGQRGIFNNVTLDGGDYNNGFFGEQLGGQRAAI
                     DITLEAVKEFQVVATGASAEFGRTAGGIINVITKSGTNDVHGSAFYFQRLEALTANTS
                     DGKPLRDFHREQYGATIGGPFRRDKAFYFFAFEGIRENLTRANLSEQIGDTPCPIAAP
                     TIIANEALINGNEDCQRLALINFFRATRDQEEGLPIPHKINNQAFFAKVDFDLNNANQ
                     LAVSYNFDYSKNTNQTFDVSTYGNSANGIEGPSKINVVRVNLFTTVSPTKLNEGHFSY
                     SRELRPRSAVPSNVPADTAMGFATTFRFGFPFFLEPNVDELLWRTHLKDNFSIVTGNH
                     TFKFGGEWLHTLNDQVFRGFFTGRYIFDSVTGFLRYASPAAAGGFGPSTVGCSDGTFV
                     TAPDTCAGGATPNGGPLLLYLQGAGLTGPATDAAGASKITNEDLALFAQDSWKIWPNF
                     TLNYGLRWEAQIFPEPVVDPSQTAYGIFLNDPRFPSDGTLPSQKKQFQPRLGFAWDVS
                     KVGKSVIRASAGIYYARQNMLSQVGSITTNGVQQQTIFLNTPIISSGVPGPVWPDVVT
                     PPSTVTPCVAGGISNPFPCFSGVRVFSRDYANPRIYTANVGFEQELAEDWSLYLDYTH
                     SKAVHLTRFLNINRNGFFEPFLGEVMVTSAVGRSVYDGFTAGMRKRFSRRYQLEWNYT
                     LSKDKDDDSNERDPFTDRAFDINNLQLDYALSDRDIRHKFNFFTFVQMGWGFEGNFRV
                     QGRTAQPITPGVRTATNRNTARKDNEYFSFDWRLQRPITLNERFSIIPIIEMFNTFNN
                     ANNINPLVTPGLFNFDGFLRQGVGDPRQVQLAVKLTF"
     gene            4221..>4728
                     /locus_tag="VFO72_03455"
     CDS             4221..>4728
                     /locus_tag="VFO72_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9478374.1"
                     /translation="MIYVAAAWIWQVSEGLLGRVWTYLTQQFTFGRITVSVSSVIVGI
                     LAFTITILIARYSSIFIERRIARRQHIDPGLRYTISTLIRYVVIIVGSLVAVKQAFNV
                     DLTSIAVIFTALSVGIGFGLQYIAADIASGFILLFERPIRVGDRVTIGEDEGDVQSIK
                     LRTTIVTTN"
BASE COUNT         1012 a         1224 c         1357 g         1135 t
ORIGIN      
        1 tgcgcggcgt atggggttgg acactgctcg cgatcatctg gtgtctcgct atcgcgggcg
       61 tcgtcatcaa ggcgctgcca cgtacacggc actcctggat ttcgatggtg ctgtacctcg
      121 tgatgggctg gctcgcaatc gttgcgatta agcccatcat ggagctcgtg ccggtgcccg
      181 gcatcctttt gatactcgcc ggcggtttcg cctacaccag cgggctcgcg ttcttcgccg
      241 caagccgctt gcgctacaac cacttcattt ggcacctgtt cgtcattgcc gggaccacgt
      301 tccacttctt cgcggtgctt tggtacgccg catagcggaa accgatagac ggcaaaattc
      361 acttcgtctt gtactgaaac tacgattagc gcgctggatg gagatcaaaa actggtgatt
      421 tcgtttcgcc ggatcgtgaa ctctcagagt caatatccca cctgaaagct cactcgattc
      481 aagcgagctg cccgatgcgg tggtcagttc gtatcctgct gggatcggca tccggaattc
      541 atcagcttcg ccctgaacaa cgttcacgtc gcacaacgct gtgaggcgta attgcgaatc
      601 gccaactgaa accaccgttt tgatgtctga aagaaagcga accttacgtt gcgcaacggg
      661 cgctgcgacc tctcgagtgg cccaccaaac gcgtgcgggt tttccgggct tgagtgacgc
      721 ttcaatcact gtatagccgt tctgggcgct gcgtttggta acgattccgg gttcaacctg
      781 aacgttcgcg tgattacccg gcggttccaa actcagcagt gaactaccgg ccagggcacc
      841 tgcagcatga acgaagcgcg gcccggatga gatgagccac ggacgaacac gaataaacac
      901 tcgcggaggc tacggctcgg ttcgtgttcg tccgtgtggc tctgtactaa aaggttagtt
      961 tcacagccaa ttgtacctgc cgcggatcgc cgacaccctg acgcaaaaac ccgtcgaagt
     1021 taaatagtcc cggcgtgacc agcgggttga tgttgttggc gttgttgaag gtattgaaca
     1081 tctcgatgat cggaatgatc gagaagcgtt cgttcagagt gatcggccgt tgaaggcgcc
     1141 aatcaaacga gaaatactcg ttgtccttgc gcgctgtgtt gcggttggtg gcggtgcgca
     1201 cgccaggcgt gatcggttgt gccgtccggc cctgcacgcg gaagtttcct tcgaatcccc
     1261 aacccatctg cacaaacgta aagaagttga acttgtgacg gatatcgcga tccgagagtg
     1321 cgtaatccag ctgaaggttg ttgatatcaa acgcgcgatc ggtaaacgga tcgcgttcgt
     1381 ttgaatcatc gtccttgtct ttcgagagcg tgtagttcca ctcgagttga taacgccggc
     1441 tgaaacgttt tctcatgccc gctgtaaacc cgtcgtagac cgaccttccc acagcgctcg
     1501 tgaccatgac ctcgccgaga aacggctcaa aaaatccgtt gcgattgatg ttcaggaagc
     1561 gcgtaaggtg gacggccttc gagtgcgtgt agtcaagata aagcgaccaa tcctcagcca
     1621 gttcctgttc gaaacctacg ttcgccgtgt agatgcgcgg atttgcatag tcgcgactaa
     1681 agacgcgcac gcctgagaaa cacggaaacg gattgctgat tccgcctgcc acgcagggcg
     1741 tgacggttga tggcggtgtc acgacatcgg gccaaacggg accgggaact ccagacgaaa
     1801 tgattggcgt gttcagaaag atcgtctgct gctgcacgcc attggtcgta atcgatccga
     1861 cttgcgagag catgttctgg cgagcgtagt agattcccgc gctcgctcgt attacagact
     1921 taccaacttt cgagacgtcc caggcaaatc cgagtcgcgg ctgaaactgc ttcttctgac
     1981 tcggcaaagt gccgtcggaa ggaaagcgcg gatcgttcag gaagattcca tacgccgttt
     2041 gactcgggtc aacgaccggt tccggaaaga tttgtgcttc ccagcgcagg ccgtagttga
     2101 gcgtgaaatt gggccaaatc ttccacgagt cctgtgcgaa aagtgccagg tcttcgttgg
     2161 tgatcttcga ggcgcccgcc gcgtcggtag ccgggccggt caagcccgcg ccttgcaggt
     2221 aaagcagcaa tggaccaccg ttgggtgttg cgcccccggc gcaagtatcg ggggcggtaa
     2281 cgaaagtgcc atcactgcag ccgaccgtgc ttggaccaaa cccacctgct gcggccggtg
     2341 acgcgtaacg cagaaatcca gtcacgctgt cgaagatgta acggccggtg aagaaacctc
     2401 ggaagacctg atcgttgagt gtgtgcaacc attcaccacc gaatttgaac gtgtggttgc
     2461 cggtgacgat cgagaagttg tctttcaaat gtgtgcgcca caaaagctca tcgacgttcg
     2521 gctcgagaaa gaatgggaaa ccaaacctga acgtggtcgc gaaacccatc gccgtgtctg
     2581 ccggcacgtt cgaaggcacg gccgaacgcg gacgcaactc acggctgtaa gagaagtgtc
     2641 cttcgttgag tttcgtcggc gaaacggtcg tgaaaagatt gacccgaacg acgttgatct
     2701 ttgatggacc ttcgatgccg ttggcggagt tgccgtaagt cgaaacgtcg aacgtctggt
     2761 tggtgttctt cgagtagtcg aagttgtacg agacggccag ttgatttgcg ttattgagat
     2821 cgaaatcgac tttcgcgaag aacgcctgat tgttgatctt gtgtggaatt ggcaatccct
     2881 cttcctggtc tcgcgtggcg cggaagaagt tgatcagcgc gaggcgctgg cagtcttcgt
     2941 tgccattgat caacgcttcg ttggcaatga tcgttggcgc ggcaattgga caaggcgtgt
     3001 cgccaatctg ttcactcagg ttcgcacgag tcagattttc gcgaatgcct tcaaaggcga
     3061 aaaagtaaaa cgccttgtct ctgcggaacg gaccgccgat agttgccccg tattgctcgc
     3121 gatgaaaatc tcgaagtggt tttccgtccg atgtgtttgc ggttagagct tcgagtcgtt
     3181 ggaagtagaa cgcgctacca tgaacgtcgt ttgtgcctga cttggtgatg acgttaatga
     3241 tgccgccggc agtgcgacca aactctgcgc tggcacccgt cgcgaccact tgaaactctt
     3301 taacagcttc cagcgtgatg tcgattgccg cacgctgtcc gccgagttgc tcgccgaaga
     3361 aaccgttgtt gtaatcacca ccatcaagag tgacgttatt gaagatgccg cgttgaccgg
     3421 cgaaattgat ctcgtcgccg tcgggacctt gcgtgatgct gacgccgggc gtaagtgtaa
     3481 gcaggtcttc gaacttgcgg ccgagaatgg gtgttgtgct gaccgccgtc tcgtttatgg
     3541 tgctgctgac ttgtgtcttc agagtatcga ctgttggcgt gtcggtgatt gtgacgcgtt
     3601 cggcaacctg ggagacggtc aaggtcacgg gaatattctt cgcttcgcct acagtcaatt
     3661 gagttctttc aagaaccccg gtggtaaaac cttgtttaga aacagtcacc gaataattac
     3721 ccggctgtaa cgcaagagcg acaaaacgac cgttatcgtc tgttgtaccg gtgcgagtga
     3781 ggttcgtatc gaggtttctt gtttcaacgc tggctccggg gataacggcg ccgtttgcgt
     3841 cactgacgac aacctcgatc gtgccggtag tactttgcgt ttgcgcggca attgccggag
     3901 cgaacaagac gatcaaaatc aagctggtag caaatgtttg aagtctgggg aagctgcgaa
     3961 agacaggggt ccgcatagtt ttctcctata gttaggtcct tttgggatgc tgatgtaatg
     4021 tcaggtgtat tcaagttggt ggaccgtggc tagattagtg agagccaagt attgtgtcaa
     4081 tgaagttctt agttcgtaaa ttaatggcgg tttgaacctg ggaccgcagg tgttgttgcg
     4141 cctgcaaagc aatgatcggg cactgccgtg agttgcaagc gagataccgg cgtcccaggt
     4201 agaggaggta tgtttttttc atgatttatg tggcggccgc gtggatttgg caggtctctg
     4261 agggcttact cggtcgcgtt tggacttacc tcacccagca attcacgttc ggcagaatca
     4321 ccgtctctgt ttccagcgta atcgtcggca tactcgcttt cacgataacg atcttaatcg
     4381 cgcgctactc gagcatattc attgaacggc gaatcgcccg ccggcagcac atcgatccgg
     4441 gtctgcgtta cacgatttct acactgatca ggtatgtcgt gatcattgtc ggctcactgg
     4501 ttgcggtcaa gcaggcgttt aacgtcgacc tgacctcgat tgccgtgatc ttcacagcct
     4561 tgtcagtcgg catcggcttt gggctccagt acattgcagc ggacattgcc tccggtttca
     4621 tactgctttt cgaaagaccg attcgcgttg gcgatcgagt cacgatcggt gaagatgaag
     4681 gcgacgtgca gagcatcaag ctgcgcacga cgattgtcac gaccaacg
//
LOCUS       DASTEY010000193         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1190976, whole genome shotgun sequence.
ACCESSION   DASTEY010000193 DASTEY010000000
VERSION     DASTEY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190976"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1986)
                     /gene="groL"
                     /locus_tag="VFO72_03460"
     CDS             complement(358..1986)
                     /gene="groL"
                     /locus_tag="VFO72_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9478375.1"
                     /translation="MPAKDLKFREEARNAMLQGVDTLANVVRVTLGPKGRNVVLGKKF
                     GPPVITKDGVTVAKEIELQDKYQNMGAQMVREVAVKTNDKAGDGTTTATVLAQAIYRE
                     GVKNVTAGANPMSLQRGIQLATEQVVGELERMSKKVKSKEELTNVATVSANNDRVIGQ
                     LISEAMEAVGKDGVVTVEESKTLQTELDIVEGMQFDRGYLSPYFITNPDRMEAILDEP
                     FILLYEKKISAMRDLIPLLEQTAGSGRALLIIAEDIEGDALATLVVNRLRGTIKVCAV
                     KAPAFGDRRKAILEDLAVLTGGKVITEDLGIKLESVTMADLGTAKRVIVDKESTTIVE
                     GGGDRKAIQGRVSMIRNQIEETTSDYDREKLQERLAKLAGGVAVLKVGAPTETAMKEI
                     KMRVEDALNATKAAAQEGIVVGGGVALMRAARVLDNHDAEDVDVRTGVKIVRRALQEP
                     LRRIAENAGIDGAIVVGQVEQLKGNKGLNAVTGQIEDLTAAGIIDPTKVVRTALQNAA
                     SIAGLLLTTDAAVTELPEKKKPAPPMPGGDDYDY"
     gene            complement(2032..>2340)
                     /locus_tag="VFO72_03465"
     CDS             complement(2032..>2340)
                     /locus_tag="VFO72_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9478376.1"
                     /translation="LERAIEAAEKDGSNDSIVQGVRSIANRFHNALASAGVEPIASVG
                     EPFDPELHEAVDTAEADPEMDGIVISEYSRGFRMGDRLLRPARVKVGRANPQTTQISA
                     "
BASE COUNT          468 a          687 c          586 g          599 t
ORIGIN      
        1 agcttgaaga atatgttcga ccgaaacgaa gttctggtcg cgacggcgcg actcgttcag
       61 cgcgtgttcc aaaatgcgct gtccgctgtc gccaaactta tctttgtatg aacctaaatc
      121 aaccataatt cttttacgac cgtcgttcga aatccgatcc agagttacaa ggattttttt
      181 gcgactgtac tactcaactt tcaaacaacg cgtgagatcc aaacgcgtac gttggatgtt
      241 gccagcataa gcgccgttgc gtttcgatgc aataactaac tgcggcggca gtatccctgc
      301 aattttcctg ccgcgcgcgg tgagtacaaa tgtaaagatt gttgctaagt ggtgtcgtta
      361 atagtcgtaa tcgtcgcctc caggcatggg cggggcgggt tttttctttt caggtaattc
      421 agttacggcc gcatcggtgg tcagcagcaa acccgcgatc gatgcggcgt tttgcagggc
      481 ggtgcgtaca acttttgtgg gatcgatgat tccagcggct gtcaggtctt caatctgacc
      541 ggtgaccgcg ttaagacctt tgtttccttt taactgctca acctgaccaa ccacgatcgc
      601 gccatcgatg ccggcgttct cagcaattct gcggagtggt tcctgtaacg cacgacggac
      661 gattttcact cccgtacgga cgtctacgtc ctcagcgtca tgattatcga gcactctggc
      721 cgcgcgcatg agtgcgacgc caccaccgac gacaatccct tcctgtgccg ccgctttcgt
      781 ggcgttgagt gcatcttcga cgcgcatctt aatctctttc atcgctgttt cagtcggcgc
      841 gccaactttc agcacagcga caccaccggc gagttttgcc agtcgttcct gtagtttttc
      901 gcgatcgtaa tcggaagttg tttcttcaat ctgattgcga atcattgaga cgcggccctg
      961 gatcgccttg cgatcgccgc cgccttcgac aatcgtcgtc gactctttat cgacaatgac
     1021 gcgcttcgcc gtgccgaggt cagccattgt cacgctttcc aatttgatac cgagatcttc
     1081 agtaatcact ttgccgccgg tgagtaccgc cagatcttcg agtatcgcct tgcgacgatc
     1141 tccgaacgcc ggcgctttca ctgcgcagac tttgatggtg ccgcgaagac gattcacaac
     1201 gagcgtagca agtgcgtcgc cttcaatgtc ttccgcaatg atcagcaagg cacggccgga
     1261 gccggctgtc tgctccagga gcggaatcag atcgcgcatc gccgagatct tcttctcgta
     1321 aagcagaatg aacggctcgt cgagaatcgc ctccatgcga tcgggattgg tgatgaagta
     1381 aggagacaaa tagccgcggt caaactgcat tccttccacg atgtcgagtt cggtttgcag
     1441 agtcttcgac tcttcgacag tgaccacgcc gtctttgccc acggcttcca tcgcctcgga
     1501 aatgagttga ccgattaccc ggtcgttgtt cgcagaaact gtagcgacgt tggtgagttc
     1561 ttccttgctc ttaactttct tcgacatgcg ttcaagctcg ccgacgacct gctcggtcgc
     1621 gagctgaatg ccgcgttgca acgacatcgg attagcgccg gccgtgacgt tcttgacacc
     1681 ttcgcgatag attgcctgtg cgagtacggt cgcagtcgtc gtgccatcgc cggccttgtc
     1741 gttggtcttg actgcaactt cgcgcaccat ctgcgcgccc atgttctgat acttatcctg
     1801 caactcgatc tctttagcga cagtcacacc gtctttagtg atcacgggcg gcccaaactt
     1861 ctttcccaga accacgttgc ggcccttcgg tcccaacgtg acgcgcacca cattcgcgag
     1921 cgtatcaaca ccctgaagca tcgcgttgcg tgcttcttct ctaaacttca gatcttttgc
     1981 cggcactctt gtgtctctcc ttttgctttc tttaggaaaa ccatccacag actacgcaga
     2041 tatctgtgta gtctgaggat ttgcccgccc gactttgacg cgcgccggac gcagcaatcg
     2101 atcgcccatg cgaaaaccac ggctgtattc actaatcact attccgtcca tctcaggatc
     2161 tgcctcggcg gtatcgacag cttcgtgcaa ctccggatcg aacggttcac cgactgaagc
     2221 gatcggctcg acgccggcgc tcgccagcgc gttatgaaaa cgattggcga tgctgcgaac
     2281 gccttgcacg atcgaatcgt tcgaaccgtc cttctcggca gcttcaatcg cgcgctcaag
//
LOCUS       DASTEY010000194         4488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_581174, whole genome shotgun sequence.
ACCESSION   DASTEY010000194 DASTEY010000000
VERSION     DASTEY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581174"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFO72_03470"
     CDS             complement(<1..350)
                     /locus_tag="VFO72_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET9478377.1"
                     /translation="MITSEVIASHNLTQQEFERIKELLGREPSFAELGIFSVMWSEHC
                     SYKSSRIHLKRLPTTGPRVIVPPGENAGVVDLGDGWCAAFKIESHNHPSFIEPFQGAA
                     TGVGGILRDIFTMGA"
     gene            complement(366..914)
                     /locus_tag="VFO72_03475"
     CDS             complement(366..914)
                     /locus_tag="VFO72_03475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9478378.1"
                     /translation="MRFSKAIVPIALCIMLLSLAAPAQKRKGRAEKPRDPIVVSPIDT
                     NELKGLLTQQRERPLLVNFWATYCDPCRDEFPDLVKIDKEYRPHALEFVTISLDDMSD
                     LKTSVPKFLDSMKATMPAYLLNVTDPEPAINLVDPRWQGDLPATYLYNEKGEVVYKHI
                     GRVNPVELREAIEKVVKKGDSQ"
     gene            complement(918..1523)
                     /locus_tag="VFO72_03480"
     CDS             complement(918..1523)
                     /locus_tag="VFO72_03480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9478379.1"
                     /translation="MKRNLSLLLASLFVIVGAIFATGARAEGEVPEPPAIGATIEDFT
                     LPDVDNKERSLKSLAGKNGTVLLFIAVQCPVSNAYNERMEKLAADYKARGIAVIGINS
                     NVAEDAAAVKAHAEQNKLSFPILKDPGNKIADRLGAKVTPEAYFLDAGNKLLYHGRID
                     NARNAAQLETSDLRNALDAALAGKAIEKSEAKAFGCSIKRA"
     gene            1620..2738
                     /locus_tag="VFO72_03485"
     CDS             1620..2738
                     /locus_tag="VFO72_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9478380.1"
                     /translation="MSEVIEQALPVPVRMDADERYTGRGVTIAFLDSGFYAHKDLTEP
                     ANRILAYHSIFSDLSSLHTPDVASWHGMMTSVVAAGNGHLSDGLYRSIAPEANLVLVK
                     IGKTGRIPESNIETGLRWVFANREKYDIRIVNISAGGDFEQSYLKNSLCQLVEEVVKA
                     GLTVVCAVGNAGLLPGHPVLPPASAPSSIAVGGLDDQNSLDRARRGMYRSSYGPTIDG
                     LQKPEVIAPGIWVAAPILPHTPTAIEAGLYAELDDAADEDLRSIIVAHNGVDKDLDAS
                     RDLNVSLLRQLITIKLREGNVINQHYKFVDGTSFSAPIVSSIIACMLEANPKLTPQQV
                     KRILIDTAERVAGVEVERQGWGVVVPRKAVESALRLRA"
     gene            2811..3053
                     /locus_tag="VFO72_03490"
     CDS             2811..3053
                     /locus_tag="VFO72_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478381.1"
                     /translation="MSENETGIGEGAAVANETAPQASNEEITYHAVEKDGVVTAIWEM
                     QGRKHLRTIDPRSRQGQDILRLYTTEHHDEAEAATS"
     gene            complement(3119..4078)
                     /locus_tag="VFO72_03495"
     CDS             complement(3119..4078)
                     /locus_tag="VFO72_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET9478382.1"
                     /translation="MDCNTKEIIVFLERDTFDVSFRKPAFDHEWVEFGETTPGEVVQR
                     LGQATIAICNKVSLRGEALAQLPNLRLIAVAATGVDNVDLAFCRNHDIAVCNTRGYAA
                     NSLPEHALMLMLALRRNLVAYRNDVRNGRWHEARQFCLLDHPIADLKGSTLGIVGFGT
                     LGKSMAELGRAIGMDVIVAERKNAATVREGRVAFGELLERSDVISLHCPLTEETKNLI
                     SAGELGQMKRDAILINTARGGLIDDHALLAALQDGRIAGAGIDVLRNEPPREGNPLLE
                     VDLPNLIVTPHNAWASRQAMQTLADQLIDNLEAFVRGEPRNLV"
BASE COUNT         1003 a         1219 c         1244 g         1022 t
ORIGIN      
        1 gcacccatcg tgaatatgtc gcgcagaatt ccaccaacgc cggtcgcggc gccctgaaac
       61 ggttcgataa acgaaggatg gttatgcgac tcgatcttga aagcggcgca ccagccgtcg
      121 ccgagatcaa cgacgccggc attctcgccc ggcggcacga tcacgcgtgg tccagttgtc
      181 ggtagtcgtt tcagatgaat gcgagacgat ttgtacgagc agtgctcgga ccacatgacc
      241 gagaagatac cgagttcggc aaaactcggt tcgcggccaa gcagttcctt tattcgctcg
      301 aattcttgct gtgtgaggtt gtggctggcg ataacttcgg aagtaatcat ttgaagtttt
      361 aacttctact ggctatcacc tttcttaacc actttctcga tcgcttcgcg cagttcaacg
      421 ggattgacgc gcccgatgtg cttgtaaaca acctcgcctt tttcgttgta cagataggtc
      481 gccggcaagt cgccctgcca tcgcggatcc acgagattga tggccggctc gggatcagta
      541 acgttgagca ggtaggccgg catcgtcgcc ttcattgaat caagaaactt cggcacgctg
      601 gttttaagat cgctcatgtc gtcgagtgag atagtgacaa actcgagcgc gtgtggccgg
      661 tactctttgt cgatcttcac caggtcggga aactcgtcac ggcagggatc gcaataggtg
      721 gcccaaaagt tgacgagcaa aggtcgctct cgttgttgcg tgagtaagcc cttgagctcg
      781 ttggtatcga ttggcgacac cacgatcggg tcgcgtggtt tctcggcgcg gccctttcgt
      841 ttctgggccg gcgccgcgag actcaacaac ataatgcata acgcgattgg aacgatcgct
      901 tttgaaaatc tcatttccta cgctctctta atactgcaac caaacgcctt ggcttctgac
      961 ttttcaattg ccttgccggc cagcgcggcg tctaaagcat tgcgcagatc gctggtttcg
     1021 agttgggccg cgttacgagc gttgtcgatg cgaccgtgat aaaggagctt gttgccggcg
     1081 tcgaggaagt aagcttcggg tgtgacctta gcgccgagtc tgtcggcgat cttgttacct
     1141 ggatccttca ggatcgggaa gctcagtttg ttttgttccg cgtgagcttt aacggcggct
     1201 gcgtcttcgg cgacgttcga gttgataccg atgacagcga tgcccctggc tttgtaatct
     1261 gcagccagtt tttccatgcg ctcgttgtag gcattcgaca ccggacactg cacggcaatg
     1321 aacagcagca cggtgccgtt ttttccggcc agcgacttga gcgagcgctc cttgttatcg
     1381 acatcgggga gcgtaaaatc ttcaatcgta gcgccaatcg cggggggctc gggaacttca
     1441 ccttcagcac gagcgccggt ggcgaaaatg gcgcccacaa tcacaaagag cgacgccaat
     1501 aacaacgaca aatttctctt catttacaag tctccttgaa cgcaactaga gtgagttcac
     1561 agcttacaca gataagcgat tttgggcaaa tctgtgtagt cggtgtaatc tgtgagtgaa
     1621 tgtctgaagt cattgagcag gcgctgcccg ttccggtgag gatggacgct gatgaacgct
     1681 ataccgggcg cggcgttacc attgctttcc tcgactccgg cttctacgcg cacaaggatc
     1741 tgaccgaacc agccaatcgc atcctcgcgt atcacagtat tttctcggac ctctcgtcgt
     1801 tgcatacgcc tgatgtcgcc agttggcacg gcatgatgac gtctgtcgtg gcggcgggaa
     1861 acgggcatct ttctgacggt ctttaccgga gtatcgcccc ggaagcgaac ctcgtgctcg
     1921 tcaagatcgg gaagaccgga cgtattccgg agagcaacat cgaaactggc ctgcgttggg
     1981 tatttgcaaa ccgtgaaaag tacgacatcc ggatagttaa catttctgcc ggcggcgact
     2041 tcgaacagtc gtatctcaaa aactcgctgt gccagctagt ggaagaagtt gtgaaggccg
     2101 gattgacggt cgtgtgtgct gttggcaatg cgggcctctt gccgggtcat ccggttttgc
     2161 cgcctgcgag tgcgccgtcg tcgatcgctg ttggtggtct tgacgatcaa aactcgctcg
     2221 atcgagcgcg gcgaggaatg tatcgctcgt cgtatggtcc aactatcgac ggactgcaaa
     2281 agccggaagt gatcgccccc gggatctggg tcgccgctcc aatcctgccg cacacaccta
     2341 ccgccatcga ggccggactc tacgccgagc tcgacgacgc cgcagacgaa gatctgcgca
     2401 gcatcatcgt tgctcataat ggagtcgata aagatctcga tgcgtcgcgc gatctcaacg
     2461 tgtcgctgtt gcgacagttg atcacaatca agctgcgcga gggcaacgtt atcaaccagc
     2521 attacaaatt tgtggatggg acatccttct ccgcgccgat cgtttcgtcg attattgcgt
     2581 gcatgttgga agcgaatccg aagctgacgc cgcaacaagt gaagcgcatt ttgatcgaca
     2641 ccgcggagag agttgcgggc gtcgaggtgg agcgacaggg atggggcgtg gtcgtgccgc
     2701 ggaaagcagt cgagtcagct ttgcgcttgc gtgcgtaaat ggcgtgtgca acaattcgcc
     2761 tcacctaaca aagacccatc gcaagcagat tggagaatat ttgagttcgt atgtctgaga
     2821 atgaaaccgg aataggtgaa ggcgcggccg tggcgaacga aaccgcgccg caagcgagca
     2881 acgaagagat tacttatcac gcagtggaga aagatggagt agtcacggcc atttgggaaa
     2941 tgcaaggacg taagcacctg cgcacaatcg atccacgctc acgccaggga caggacatcc
     3001 ttcgtcttta caccaccgag catcacgacg aagccgaagc tgccacctcg tagacgggcc
     3061 acaaaaaggc acaaaaatca ccaaagatta attaatacca ttttgtgctt tttgtggctt
     3121 aaaccaagtt ccgcggctcg cctctcacaa acgcttcaag gttatcgata agctgatcgg
     3181 ccagcgtctg cattgcctgg cgactggccc acgcattgtg tggcgtcacg atcaggtttg
     3241 gcagatcgac ttcgagcagc ggattgccct cgcgtggcgg ttcgttgcgc agcacgtcaa
     3301 taccggcgcc cgcaatccgt ccgtcttgca atgctgccaa caacgcgtga tcgtcgatca
     3361 atccgcctcg agcggtattg atcaaaattg cgtcacgttt catttgcccg agctcaccgg
     3421 cgctaatcag gttttttgtt tcttccgtga gaggacaatg caatgagatc acgtcactcc
     3481 gttccaataa ctctccaaaa gccacgcgac cttcgcgcac ggttgcagcg ttcttgcgct
     3541 cggcgacgat cacgtccatg ccgattgcgc gcccgagttc ggccattgac ttcccgagtg
     3601 ttccgaaacc cacaattccc aaagtgctgc ccttgagatc cgcgatggga tgatcgagga
     3661 ggcagaactg cctcgcttca tgccagcgtc cgttcctaac gtcgttgcga taggcaacga
     3721 gattgcggcg caaggcgagc atgagcatta gtgcgtgttc gggcagtgaa ttagcggcgt
     3781 aaccgcgagt gttacaaacg gcaatgtcat ggttacggca aaacgccaaa tccacgttgt
     3841 ccacgcccgt agcggcgacg gcgatcagcc ttaaattcgg caactgcgca agcgcctcac
     3901 cgcgcagtga aaccttattg cagattgcga tggtcgcctg gcccaaccgc tgtacgacct
     3961 cacccggcgt ggtctcccca aactcgaccc actcatggtc aaacgcgggc ttacggaaag
     4021 agacgtcgaa agtgtcgcgt tccagaaaga cgattatttc tttggtgtta cagtccatca
     4081 cgcatccgag aatttgaaga ataacgaaat ttttggcgaa aagctgatgg taatttggcc
     4141 gcccttcgta cgcccgccgt tgctaagttc ctgcgccgca agcgttagcc aagatgaacg
     4201 taggctgata gttgccggaa taatcgttgc atgcaaccga accgtctttc tttcctacgc
     4261 gcgcgtttag tcctaacccg aagtaacaac ctttagtgat caaataagga ggattgtcca
     4321 tgagaagccc actacgcttg gcaagcgcag ctctcttgag cgtcttgtgc gcgataagtg
     4381 tgtttgccca agcgagccaa acaggaggta taaccggcgt cgtcactgac tccggaggcg
     4441 ccctcgttac gggcgcgaca gtcgaggtaa ttaacgagag caccggaa
//
LOCUS       DASTEY010000195         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_630870, whole genome shotgun sequence.
ACCESSION   DASTEY010000195 DASTEY010000000
VERSION     DASTEY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630870"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFO72_03500"
     CDS             complement(<1..746)
                     /locus_tag="VFO72_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9478383.1"
                     /translation="MNADLGSVENGSNLWRRYRGLPRNVVAIGLVSLLNDASSEIIYP
                     LLPVFLASSLGASAGVIGAIEGLAESISSLLKLFAGYLSDRLGKRKLLVVAGYSLASI
                     ARPLLAFARSWSQVLAIRLTDRVGKGIRTAPRDAMIADAVRVEQRGLAFGFHRAMDHG
                     GAVIGPLLGYLLVVLFVANSHSPTTEEFSKIFLAASVPALIAVMVAIFFMRESPVHVH
                     HGAQMAKLSLRGFDSNFKRFLVILALFTLS"
     gene            complement(762..1181)
                     /gene="queF"
                     /locus_tag="VFO72_03505"
     CDS             complement(762..1181)
                     /gene="queF"
                     /locus_tag="VFO72_03505"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET9478384.1"
                     /translation="MATLLPPNQQCLDIQSTPRAEMNLFPLDTFPYEFPGSEIKINFE
                     ITEFTAICPFSDFPDFATIRIEYVPNETCIELKSLKLYINSFRNIKIFHEHVINIILE
                     DFVASCDPLRVDIEGDFHVRGNIKTIVRASYTKDTAV"
     gene            complement(1224..1988)
                     /locus_tag="VFO72_03510"
     CDS             complement(1224..1988)
                     /locus_tag="VFO72_03510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9478385.1"
                     /translation="MVFPLYDDNADRATTPLVNYAIIALNIFVFVVLQKLGSDTGFTY
                     SFSTVPQEILSGTDITTPARVLEHPMTGQRVLIPGLGETPGSVYLTLFTSMFMHGGIA
                     HIAGNMLFLWIFGDNIEDRLGHIKYVIFYLLCGLVASLAHVFTTGVFATDPESLLVPS
                     LGASGAISGVMGAYLLLHPKRRVTVILFRFLTDVPAYVAIGIWFAFQLISGLGLLGGG
                     SELGGVAYAAHIGGFIAGVVLIKFFDRGNRNRAPAY"
BASE COUNT          593 a          623 c          614 g          431 t
ORIGIN      
        1 tttgaaagcg taaagagcgc gaggatcaca agaaaccgtt tgaagttact atcgaatccg
       61 cgcaacgaga gcttcgccat ctgcgcgccg tgatgcacgt gtaccggcga ctctcgcata
      121 aagaagatgg caaccatcac cgcaatcaac gccggtacgg aagccgccag gaatattttc
      181 gagaactctt cagtagtcgg agagtgtgag ttcgcgacaa agagtacgac cagtaaatat
      241 ccaagcagtg gaccgataac cgcgccgccg tggtccatcg cgcgatgaaa accaaacgcg
      301 aggccgcgtt gttcgaccct caccgcgtcc gcaatcatcg catcgcgcgg cgccgttcta
      361 atccctttac cgacgcgatc tgtaaggcgg attgcgagca cctggctcca cgagcgcgcg
      421 aatgccagca gcggccgggc gatcgatgcg agtgaataac ccgcaacgac gagtaacttt
      481 cgtttgccga gacgatcgct aagataacca gcaaacagct tcaacaaact cgaaatcgac
      541 tcggcgagcc cctcgatagc gccaatcacg cccgccgaag cacccaaact cgacgcgaga
      601 aaaaccggca gcagcggata aatgatctcg ctcgaggcat cgttgagcag actcaccaaa
      661 ccaatcgcca ccacatttcg cggcaaccca cgataccgcc gccacaaatt actgccattt
      721 tccacagaac caagatctgc gttcatctgc gtaatctgcg gctaaactgc tgtgtcctta
      781 gtgtagctgg ccctcacgat ggtcttaatg ttgccccgga cgtggaagtc gccttcgatg
      841 tcgacgcgca gagggtcgca ggaggcgacg aagtcttcca gaatgatgtt gatgacgtgc
      901 tcgtggaaga tctttatgtt gcgaaatgag ttgatgtaga gcttcaaact cttgagctcg
      961 atacaggttt cgtttggaac gtactcgatc cggatggtcg cgaaatcagg gaagtcggag
     1021 aacgggcaga tcgccgtgaa ctcggtgatc tcgaaattga tcttgatttc gctaccggga
     1081 aactcgtaag ggaacgtgtc gagcggaaag aggttcatct ccgcccgcgg cgtcgattga
     1141 atatcgagac attgctggtt tggtggcaat agcgtggcca tgggaggcga tcttaaccac
     1201 aaagacacca ggacacaaaa ttgctaataa gccggggcgc gattgcgatt tccacggtcg
     1261 aaaaacttga tcagcaccac tcccgcgatg aagccgccga tgtgcgcggc gtatgccacg
     1321 ccgcctagtt ccgaaccacc gccgagtaat cccaaaccgc tgatcaactg gaaggcgaac
     1381 cagatcccga tcgcgacata cgccgggaca tccgtgagga agcggaaaag aatcactgtc
     1441 acacgacgtt tcggatggag cagaaggtaa gcgcccatca cacctgagat cgctcccgaa
     1501 gcgcccaagc tcggaaccaa taaactctct ggatcagtcg cgaacactcc cgttgtgaac
     1561 acatgagcca atgaagcaac gagcccgcac agcaggtaga aaatcacata cttgatgtgg
     1621 cccagacgat cttcaatgtt gtcgccgaag atccaaagga agagcatgtt gccggcgatg
     1681 tgcgcgatac cgccgtgcat gaacatcgac gtgaagagtg tcagataaac cgaacccgga
     1741 gtctcgccaa gaccgggaat gagcactcgt tgaccggtca tgggatgctc aagcacacgg
     1801 gccggagtgg tgatatccgt gccactaagg atctcttgcg gcaccgttga gaacgaataa
     1861 gtgaaaccgg tatcgctgcc gagtttttgc agcactacga agacaaagat attcagggca
     1921 atgattgcgt agtttacgag gggggttgtc gcgcgatcgg cattgtcgtc atacagcgga
     1981 aaaaccatgg ccctgtaacc tcatgtcgga aatggggtaa ttctaacgct tgtcatatgc
     2041 gcagattgtg gcgtgcctgt tactaaagga tactaaaggg atactaaagc ctcaatccat
     2101 gccacgctcc gcgacgggaa cgcccgcgtg aaccagcgcc ggctcgtgcg gtctcagtaa
     2161 ttcatatcct tccgaacgcg ccacatacgt cgccgcggtg aggtcgccgg taacgttcag
     2221 cgtggtgcga cacatatcaa gcaagcgatc gacgcccaaa a
//
LOCUS       DASTEY010000196         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_152767, whole genome shotgun sequence.
ACCESSION   DASTEY010000196 DASTEY010000000
VERSION     DASTEY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152767"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1487)
                     /locus_tag="VFO72_03515"
     CDS             complement(114..1487)
                     /locus_tag="VFO72_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HET9478386.1"
                     /translation="MNTGTFSENAKLALKDTQLRGALRHATMLFGKRRREAAASLSNW
                     EDLRTQARAIKDEVLLHLDRYLETFVHNAESRGAKVHWARDATEANAIICRLTIERDA
                     RNVVKSKSMTTEEIHLNAALEAAGMQVVETDLGEYIIQLADEAPSHIIAPAIHKTKRQ
                     IAELFTTELGMPPTDDVAQLTSTARSTLRERFATADVGISGVNFGVAETGTIVIVENE
                     GNIRMTTSLPRTHIAVMGIEKLLPRFADLDLFLKLLPRSGTGQASSTYQSFITGTKRN
                     ATEEGPDELHIVLLDNGRSRMLAHPVTRQSLACIRCGACLNSCPVYQQIGGHAYGSVY
                     PGPIGSVITPQLRGIQKTAQLPYASSLCGACREVCPVKIDIPRLLLHLRGEISSKQTR
                     GRRKERLAFKVWAAIMMRPWLYEKTAVAGRLFQRWFKPPLKAWTEGRDLRPIEAESFR
                     ERWRRTR"
     gene            complement(1493..2215)
                     /locus_tag="VFO72_03520"
     CDS             complement(1493..2215)
                     /locus_tag="VFO72_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9478387.1"
                     /translation="MNVSLFVTCLVDQLWSNIGTSCVAVLRRVGCEVEFDERQTCCGQ
                     PAFNTGYRDEARQVARRFIEIFEESKADAIVSPSGSCTAMVQHFPELFREDPLWFERA
                     QAIAARTHEFSAFLVRVLKIEDVGASWQGKLTWHDACHGLRDLNIKTEPRKLIRNVRG
                     AEFIEMENAEACCGFGGTFSVKYPEISVAILDQKIEAVERAGVQAVVSGDASCLMQIG
                     GRLSRLGSKVDVMHLAELLNHR"
BASE COUNT          502 a          750 c          618 g          553 t
ORIGIN      
        1 ccgactccac cgctggtcta aacggcgatg tacacggatt ggtttggctc ggtgtctcac
       61 gagcgtcata aggcgcactc gtcgccaggt gcgtgcgaat ggagcgcatg atctcagcgc
      121 gtgcgtcgcc accgttcacg aaaactctcc gcttcaattg gacgcaaatc gcgcccctca
      181 gtccacgcct tcaacggcgg cttaaaccag cgttgaaaaa gacgtccggc cactgccgtc
      241 ttctcataaa gccacggccg catcatgatc gccgcccaca ctttgaacgc caaacgctct
      301 ttccggcgtc cacgcgtttg cttgctgctg atctcgccgc gtaagtgcag caacaatcgc
      361 ggaatgtcga tcttcaccgg acacacttca cgacacgcac cgcacaaact cgacgcgtaa
      421 ggcagttgcg ccgtcttttg aatccccctc aactgcggcg tgatcaccga cccgatcggt
      481 ccgggataaa ctgaaccata cgcgtggccg ccaatctgct gatacaccgg acacgaattc
      541 aaacatgcgc cgcatcgaat gcacgccagc gactgtcgcg ttaccggatg ggccagcatt
      601 cgcgaacgac cattgtcgag cagaacgatg tgcaattcgt ccggaccttc ctcggtcgcg
      661 ttgcgtttgg tgcccgtgat gaacgactgg tacgtgctcg atgcctgccc ggtgccggaa
      721 cgcggcagca gtttcagaaa caggtcgaga tctgcaaacc gcggcaacag tttttctatg
      781 cccatcaccg cgatgtgcgt tcgcggcaga ctggtcgtca tacgaatgtt gccttcgttt
      841 tcgacgataa ctatcgtgcc ggtttccgcg acgccaaaat tgacgccact gatgccgaca
      901 tcagccgtgg caaacctctc gcgcaacgtc gagcgcgcgg tgctggtaag ttgggccacg
      961 tcgtcagtcg gcggcatacc gagctcagtc gtaaacagtt ccgcgatctg ccgtttggtt
     1021 ttgtgaatcg cgggcgcgat gatgtgcgag ggagcttcat cggcgagttg aatgatgtac
     1081 tcgccaagat cggtctcgac aacctgcatt ccggcggctt ccaacgctgc gttcaggtga
     1141 atctcttccg tcgtcatgct tttacttttg acgacattgc gcgcgtctcg ttcgattgtc
     1201 aggcggcaga tgatagcgtt tgcttcggtg gcgtcgcggg cccagtgaac tttcgcgccg
     1261 cgactctcgg cattgtgcac aaacgtttcc agatagcgat cgagatgcaa cagcacttca
     1321 tctttaatcg ctcgcgcctg agtgcgcaga tcttcccagt tgctcagact cgccgccgcc
     1381 tcgcgccttc gtttgccgaa gagcatcgtc gcgtgacgca atgcaccccg caactgcgta
     1441 tcctttagtg cgagtttcgc gttttcggaa aacgttcccg tgttcatccg tttcatctgt
     1501 ggtttagtaa ttccgcgaga tgcattacat ccactttaga tccaaggcgc gacagtcgcc
     1561 caccaatctg catcaaacaa ctcgcgtcac ccgaaacaac cgcctgaacg cccgctcgct
     1621 cgaccgcctc gatcttctgg tcgagaattg cgaccgagat ttccggatat ttcactgaga
     1681 aagtgccgcc aaacccacag cacgcctccg cgttctccat ctctataaac tcagcgccgc
     1741 gcacgttgcg aatcagtttc cgtggctcgg ttttgatatt gagatcgcgc aggccatgac
     1801 acgcatcatg ccaggttaac tttccctgcc aacttgcgcc cacgtcttca atcttcagta
     1861 cacgaaccag gaacgcgctg aattcgtgcg ttcgcgcagc aatggcctga gcgcgttcaa
     1921 accacaacgg atcttcgcgg aacaactcgg gaaagtgctg gaccatcgcg gtgcacgatc
     1981 cagacgggct gacgatcgcg tccgccttgc tctcttcgaa gatctcgatg aatcttctcg
     2041 caacttgccg cgcttcgtca cgataaccgg tattgaacgc cggctggccg cagcacgtct
     2101 gccgttcgtc aaactcaact tcacaaccca cccgtcgcag cacagcaacg caactggttc
     2161 caatgttgct ccaaagctga tcgacaagac aggtgacgaa cagggaaacg ttcatgacat
     2221 tttcgattgc cgattgccga ttgccgattg ccgattttcg cttgtccgtt cgctgtcttt
     2281 caatcggcaa tcggcaatcg gcaatcgaaa atatcatcac acctttctat gactgccgtc
     2341 tgttagaggt atttctccca gtgaccgagg attgtgcgga gttcgtccaa atcttgcggc
     2401 gtgacgttca caagcggcgg cct
//
LOCUS       DASTEY010000197         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_861476, whole genome shotgun sequence.
ACCESSION   DASTEY010000197 DASTEY010000000
VERSION     DASTEY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861476"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VFO72_03525"
     CDS             <1..1479
                     /locus_tag="VFO72_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478388.1"
                     /translation="KDDFGLTLLTHREPGKHGYFLLMISPKEDWSEQEYSAKDVVFVV
                     DTSGSMAEEGKMEKARAALLYGVRILRPQDRFNVISFAGEERLMEPGLIAADDKGRQR
                     GEAFVKTLKPVGGTNINGSLLASLSQFNEDNRERPKIIVFLTDGLPTVGTTNVNQIVD
                     NVRKASKPGVRLFTFGVGYDVNTALLDKLAAENGGVADYVEPKEDLEVKVSSFFTKVN
                     YPVLTNLKLDLSTVQTDLIYPRGIPDLFRGSQVTLIGRYSNQANLDSMRLTLTGQSGG
                     SSRTYTYDHLSFPLRNENNEYLPRLWATRRVGWLMEQVRTNGEQKELRDEIVDLGTRY
                     GIVTPYTSYLALESAEVSGLNVTRPPARQRGFTGGVRAQPNAPPANTGPVVSLSTGAA
                     AVQQSKRVREQQEAETLKDETRTDAVRRVGGKTFYLVDGVWTDSEFKAETRLPETALT
                     FGSEEYFAVLKQHPKLADYFSLGERVVIVFEGRVYRVSAANP"
     gene            1582..2565
                     /locus_tag="VFO72_03530"
     CDS             1582..2565
                     /locus_tag="VFO72_03530"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HET9478389.1"
                     /translation="MNIRSLNLVRFAAAALVLFTLATSSQAEGWAPIKLNTGGNDLNT
                     VYFIDSKRGWVGGDEGYLSRTEDSGQSWVRQSVSTKAAINDIYFRDKEAGFFLAGNSI
                     FITRDNGVTWRQSKIFSPEEFDGAEVELYSVRFSSKKKGWVVGSVSKRDRVVDSVLVY
                     TDDGGETWRRQRTPSRLELIHIDFVSDKRGWIVGHGGTILFTRDGGQSWTKQNAGVNG
                     TLYHVDFRDDKKGWVVGERGVLLRTTDGGETWTPVPTNVNVTLLSVEFVNSDDGWAIG
                     RGGTILRTSDKGRTWTQQQSTTKQNLYSLNFNKKIGWAVGGEGVILRYERD"
BASE COUNT          606 a          656 c          775 g          540 t
ORIGIN      
        1 aaagacgact ttggtttgac tctgctcacg catcgcgagc cgggcaagca cggttacttc
       61 ctgctgatga tttcgccgaa ggaagactgg tccgaacagg agtactcagc gaaggacgta
      121 gtcttcgttg tcgacacttc cggatcgatg gctgaagaag gaaagatgga gaaggcgcgc
      181 gccgctttgc tctacggcgt caggattctg cgaccgcaag atcgcttcaa cgtgatttcg
      241 tttgccggtg aagagcgctt gatggagccc ggattgatcg cggctgacga caaaggccgc
      301 caacgtggag aagcgtttgt aaaaactttg aagcccgttg gaggaacgaa catcaatggc
      361 tcgctgttgg cctcgttgag tcagttcaat gaagacaatc gcgagagacc gaagatcatc
      421 gtgttcttga ccgacggttt gccgacggtg ggaacaacca acgttaacca gatcgtggat
      481 aacgtgcgca aagcgagcaa gcccggcgtg cggttgttta cgtttggcgt tggttacgac
      541 gtcaacacgg cgctgctcga caagctggct gctgaaaacg gcggcgtcgc cgattacgtc
      601 gagccgaaag aagatttgga agtaaaggtc tcgagtttct ttacgaaggt gaactatccc
      661 gtgctgacga atctcaagct cgacttgtcc accgttcaaa cggatctgat ttatccgcgc
      721 ggcattccgg atcttttccg cggttcgcag gtgacgctga ttggacgtta cagcaaccag
      781 gcaaatctcg attcaatgcg actgacactg accgggcaaa gcggtggttc gtctcgcact
      841 tacacgtacg accatctgag ttttccgctg cgcaatgaaa acaacgaata cctgccgcgt
      901 ttgtgggcga cgcgtcgtgt cggctggttg atggagcagg tgcgtaccaa cggcgagcag
      961 aaggagttgc gcgatgagat cgtcgatctc ggaacgcgct atggaattgt gacgccgtac
     1021 acgtcctatc tggcgttgga gagcgcagaa gtgagcgggc tgaacgtcac gaggccgcca
     1081 gcgcggcaac gcggtttcac gggtggcgtt cgggcacagc caaatgcacc tccggccaac
     1141 accggtccgg tcgtatcact ctcgaccggt gctgccgctg tccagcaaag caaacgtgtc
     1201 cgcgagcagc aggaagctga aacgctgaaa gatgagacgc gcacagatgc ggtgcggcgt
     1261 gttggtggca agacgtttta tctggtcgat ggagtgtgga ccgactcgga attcaaagcc
     1321 gaaacgcgtt tgccagagac cgcgttgacc tttggcagtg aagaatactt cgcggtgctg
     1381 aagcagcatc cgaagctggc ggattacttc tcgcttggcg agcgcgtcgt gattgtcttc
     1441 gaaggtcgcg tctatcgtgt aagcgccgca aatccatagg cctcgtctct tctgtgttct
     1501 ctgtgtcagg acgcagagga cacagaagag acattgatct ttcccggctg cggatggtac
     1561 aaagttgaac acacagccgg catgaatatt agaagcttaa acctggtgcg gtttgccgcc
     1621 gcggcgctcg ttctgttcac gcttgccact tcgtcgcaag ccgagggctg ggcgccaatc
     1681 aaactcaaca ccggcggaaa cgatctcaac accgtctatt tcatcgacag caaacgtggc
     1741 tgggttggtg gtgacgaagg atatctaagc cgcaccgaag acagcggtca atcgtgggtg
     1801 cgccaatccg tcagcacaaa agccgcaatc aacgatatct acttccgcga caaggaagcc
     1861 gggttcttct tagccggaaa ttccatcttc attactcgcg ataacggcgt cacatggcgg
     1921 cagtcgaaga tcttttcgcc tgaagaattc gacggcgctg aggttgagct gtacagcgtg
     1981 cgcttttcca gcaagaagaa aggctgggtc gtcggttcag tcagtaagcg cgatcgtgtt
     2041 gtcgacagcg ttctcgttta caccgacgat ggtggcgaaa cctggcgacg ccaacgcacc
     2101 ccgagccggc tggagttgat ccacatcgat ttcgtgagtg acaagcgagg ctggatagtc
     2161 ggccacggag gcacgattct gtttacgcgc gacggcggac aaagctggac taaacagaat
     2221 gcaggggtaa acggcacctt gtatcacgtc gattttcgcg acgataagaa aggttgggtt
     2281 gttggcgaac gcggcgtgtt gctgcgcaca accgacggcg gcgagacctg gacgccggta
     2341 cccacgaatg taaacgtgac ccttctcagc gtagagtttg taaatagcga tgatggttgg
     2401 gcaatcggac gcggtggcac gattcttcgc accagcgata agggaagaac gtggacgcag
     2461 cagcagtcaa caacgaagca aaacctctac tcgttgaatt tcaacaaaaa gataggctgg
     2521 gcagtaggcg gcgaaggagt tatcctccgc tacgagcgag actagtcaaa aggcttt
//
LOCUS       DASTEY010000198         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1092457, whole genome shotgun sequence.
ACCESSION   DASTEY010000198 DASTEY010000000
VERSION     DASTEY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092457"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFO72_03535"
     CDS             complement(<1..711)
                     /locus_tag="VFO72_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET9478390.1"
                     /translation="MPAPNIDTIVSLAKRRGFVFPSSEVYGGLGSAWDYGPLGVELSN
                     NIKQSWWRWLVYERDDIEGIDSSIILNRLVWKYSGHEDTFHDPLVDCRECKTRMRADK
                     IEGKCSNCGSTNLTDPRYFNMMFRTLVGAAAEEDDPAALAYLRPETAQGIFINFVNVQ
                     TTMRRKLPFGVAQIGKSFRNEITPGNFVLRTREFEQMEMEYFVRPDEEHLKLVDEWVE
                     LYVEWFKGLGIDGRNLNIH"
     gene            complement(788..949)
                     /locus_tag="VFO72_03540"
     CDS             complement(788..949)
                     /locus_tag="VFO72_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478391.1"
                     /translation="MAEEGGGGGSTGIVAVLVIFVIIVLAALFIFRDRIFRGGGTQNI
                     DVNIQTPSK"
     gene            complement(1054..2067)
                     /locus_tag="VFO72_03545"
     CDS             complement(1054..2067)
                     /locus_tag="VFO72_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478392.1"
                     /translation="MSTRNFIFKHFRFISIAVLTLLVLAVYSSPEVHAKKKAKYGTLK
                     ILTTPGGLLLTIDGQPRGETLTKYRGFDLEPGVHNVVVKLPNGTLWARQIDVPAGRVK
                     CVVVNYRPLPPPPKSPCPFPVNVSAPQSVGEGEIITFTADVSYGGPSDLKYNWTISPS
                     RARIMSGAGSPTITVDSTGFGGQRIIATLAVDDGSGDPACRQIAQAVSVVAAEEKKIL
                     VAREFDECNNCTFDDQKARLDNLAIELQNDPATTGYIIAYGGRTSPIGQVEQLMTRAR
                     DYLITERQIHGSRIVVVNGGFREADSVELWVVPSGAAPPRATPTVQVGDLKPAGAVRR
                     RRS"
     gene            complement(2073..>2500)
                     /locus_tag="VFO72_03550"
     CDS             complement(2073..>2500)
                     /locus_tag="VFO72_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET9478393.1"
                     /translation="NNDAPVGPGDTAKVDGVLPGLPTLQGPPQLGVEVIAEADVEGTI
                     CDCGEILIPGGGFPKWPLLFLTAVPLVFINDCDDCDENPISTPTPTPPPPTPTPTPPI
                     PEPASLLLFGTGLLAVGAGLRRRHLKMKAGQVEAARKEE"
BASE COUNT          540 a          737 c          674 g          549 t
ORIGIN      
        1 gtgaatattt aaattgcgcc cgtcgatgcc cagccctttg aaccattcga cgtaaagttc
       61 gacccactcg tcgacgagct tcaagtgttc ctcatccggt ctgacgaagt actccatctc
      121 catctgctca aactctcgcg tacgcaagac gaagttcccc ggcgtgatct cgttgcgaaa
      181 cgacttccct atttgcgcaa caccgaacgg aagcttgcgc cgcatcgttg tttgcacgtt
      241 cacgaagttg atgaagattc cctgggccgt ttcgggacgc aggtatgcga gcgctgccgg
      301 atcgtcttct tctgcggctg cgccaaccag tgtgcgaaac atcatgttga aatagcgcgg
      361 gtccgtcagg ttggtcgaac cacagttcga acacttccct tcaatcttgt ctgcgcgcat
      421 tctcgtcttg cactcgcggc aatcgaccag cggatcgtga aacgtgtcct cgtgtcccga
      481 gtacttccac accaaccggt tgagaatgat tgacgagtcg ataccctcga tgtcgtcacg
      541 ctcgtagacc aaccagcgcc accacgattg tttgatgttg ttgctgagct ccacgccgag
      601 tggaccataa tcccaggcgc tgccgagtcc gccgtagact tcggaggatg gaaaaacgaa
      661 gcctcgccgc ttagcgaggg agacaatagt gtcgatgttg ggtgcaggca ttgcgaatag
      721 tatatagcca cagattacac cgattgtcat ccgggtaacc tgtggccaaa tacgcgagct
      781 tcactgctca cttactcgga gtttgaatgt ttacgtcgat gttctgcgtg ccaccacctc
      841 gaaatattct gtctcggaaa atgaacagtg ctgccagcac tatgatcaca aaaatcacga
      901 gcaccgcgac gatgcccgta ctgccgcctc cgccaccttc ttcagccatg acggtatcct
      961 ccacttaatc aactatgaca gggggcaaaa gcggggaaag gggccgccgt tttacttagg
     1021 ggcgacccca ttttactcag gatggactca gtttcaactg cgtcgacgac gcacggcgcc
     1081 ggccggcttc aaatcgccca cctggaccgt cggggttgct cgaggtggtg ccgcgccgct
     1141 cggcacgacc caaagctcaa cactgtctgc ctcacggaac ccgccgttga ccacgacgat
     1201 gcgcgagccg tgaatttggc gctccgtaat gaggtagtca cgcgcgcgcg tcatgagctg
     1261 ttcgacctgt ccaatcggac tggtgcggcc gccataagcg atgatgtaac ccgtggtcgc
     1321 cgggtcgttc tgaagttcaa ttgccagatt atcgagacgt gccttctgat cgtcgaaagt
     1381 gcagttgttg cattcgtcaa actcgcgcgc cacgaggatc ttcttctctt cagcagcaac
     1441 aactgaaact gcctgcgcta tctgccggca cgcagggtcg ccggagccat cgtcaactgc
     1501 caacgtcgcg ataatgcgct gaccaccgaa gccggtcgaa tcaactgtga tcgtgggcga
     1561 acccgcgcca ctcatgattc gcgccctcga cggactgatg gtccagttgt acttcaaatc
     1621 ggacgggcca ccgtatgaca cgtcggcagt gaaggttatg atctcgcctt cgccgacaga
     1681 ctgtggcgcc gaaacattca ccgggaaagg acacggcgac ttcggcggcg gcggaagcgg
     1741 ccggtaattc actaccacac acttcacacg tcccgccggt acatcgatct ggcgcgccca
     1801 caaagtaccg ttcggcagct tgacgacgac attatgaaca cccggctcca gatcaaaccc
     1861 gcgatacttc gtcagcgtct cgcctcgcgg ttgcccgtcg atcgtaagca acaatccgcc
     1921 gggcgtagtc agaattttga gggtgccgta tttcgctttc ttctttgcgt gaacttccgg
     1981 cgaggaatag acggcgagaa cgagcaatgt cagcaccgcg atggaaataa accggaaatg
     2041 tttgaagatg aagttccgtg ttgacacgag ttctattcct ccttcctcgc ggcttcaacc
     2101 tgcccggctt tcatctttaa gtggcgccgt ctcaggcctg ctccgactgc cagtagacca
     2161 gtaccaaaca acagcaacga cgccggttcg ggaattggcg gtgttggcgt tggcgtaggc
     2221 ggcggcgggg tcggcgttgg tgttgagatt gggttctcgt cgcagtcgtc gcaatcgtta
     2281 atgaagacga ggggcaccgc ggttaaaaac agcaacggcc atttcggaaa gccaccgcca
     2341 ggaatgagaa tctcaccgca atcacaaatc gtgccttcga cgtctgcttc tgcgataact
     2401 tcaactccaa gttgcggtgg accctggagc gttggcagac caggcagaac tccgtcgact
     2461 tttgcggtat cgcctggccc aacaggcgca tcattgtttg
//
LOCUS       DASTEY010000199         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_235052, whole genome shotgun sequence.
ACCESSION   DASTEY010000199 DASTEY010000000
VERSION     DASTEY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235052"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VFO72_03555"
     CDS             complement(<1..1692)
                     /locus_tag="VFO72_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9478394.1"
                     /translation="MRLKITFLFAFVLTIALYGYAAAQAPASQPAHAVKINFNQRVRM
                     RDGIELSADVYRPDSAGQFPVILSRTPYIKTSAGTLAIARYFVPRGYVFVAMDVRGRG
                     DSDGEFVPYRNDGRDGYDAIEWCAKQPWSTGKIGTIGGSYNGRIQWLTALEQPPHLTT
                     MIAMVTPSDPFVEWPTGLPLPMGISWYHFTAGRVSQNMNAVDWPKIHRHLPMKTMDEA
                     AGRPNRYWKEMFEHTQLDEWWEPLRYQNKFDRVKVPVLHISGWYDDEQIGTPLNFIGM
                     TTKGATPAVRHSQKLLMGPWPHAVNSTSKLGEVDFGPTAVIDLNGYWLRWFDYWLKGV
                     DNGIMREPSARIFVMGENRWVDENEWPIARTQWVKYYLHSRGRANSLFGEGTLSTSLP
                     DNEPVDNYVSDPSRPVPFITEPSFAQIGSTDDYRAIERRDDVLVYTTAPLTNDVQICG
                     PISARLFAASSARDTDFMMKLLDVWPNGYAQRLTDGMVRARFRQGMERPALIEPGRVY
                     EYSIDLWNTCQTFKKDHSIRIEVASTAFPKYDRNPHTGEALGVSTRLEVAQQKVYH"
     gene            complement(1695..2696)
                     /locus_tag="VFO72_03560"
     CDS             complement(1695..2696)
                     /locus_tag="VFO72_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET9478395.1"
                     /translation="MSESKSKNLRPKAKKKAAPAKKPELVLSGVDGADAKTATTALDV
                     RFASARAKLNGRRQRLIRAILDSADETCFLSSREMAKRYRVDATTILRTTQVLGYKTF
                     ADFAADLRQHFVARITPYTVLKAATREKRSVADHVDQALDKALDNLNILKTSLDKPKV
                     IELARTIKRSRHVLVVGLDFASSLSYYLAYGLLALGFDAEALIGSSGNLQYKVEVLTS
                     KDVLIAISFGQCLRDTVEAVQRARKQGVPTFGITDSNTSPIARYCDAYLIAPVVSPSF
                     LNSYVAPMAAISAIHVACAHIDPNRSLSRLRPTDREYASGHRWYRESKRSNGNEPQD"
BASE COUNT          621 a          866 c          749 g          586 t
ORIGIN      
        1 atgatagacc ttttgctgcg ccacttccaa tcgcgtcgac acacccaacg cctcacctgt
       61 atgcgggttt cgatcgtact tcggaaacgc agtcgaggct acttcgatcc ggatcgagtg
      121 gtccttcttg aacgtctgac acgtgttcca aagatcgatt gaatactcgt acacgcgccc
      181 aggctcgatc aacgccggac gctccatgcc ctggcgaaat ctcgctcgca ccatgccgtc
      241 ggttaatcgc tgcgcataac cattgggcca aacgtcaagc agcttcatca tgaaatcggt
      301 gtcgcgagcc gacgacgccg cgaacaaccg cgcacttatc ggaccgcaaa tctgtacgtc
      361 gttcgtcagc ggcgccgtcg tgtaaaccaa cacatcatcg cgccgctcga tcgcacgata
      421 atcgtcggtg ctgccaatct gcgcaaacga cggctccgta atgaagggca ccggacgcga
      481 aggatcgctg acgtaattat cgaccggctc gttgtccggc aaactcgtcg ataacgtccc
      541 ctcgccaaac aaactgttcg cccgaccacg actgtgcagg taatacttta cccattgtgt
      601 ccgcgcgatc ggccactcgt tttcgtcgac ccaacgattc tcgcccatca caaagatacg
      661 cgcgctcggc tcgcgcatga tgccgttgtc gacacccttc aaccagtaat caaaccagcg
      721 cagccaataa ccatttagat cgatcaccgc cgttggacca aaatccactt cgcccagctt
      781 cgaagtcgag ttgactgcat gcggccaggg tcccatcaat aacttctgac tgtgacgaac
      841 cgccggcgtt gcgcccttcg tcgtcatgcc gatgaaattc agcggcgtgc caatctgctc
      901 gtcatcgtac caacccgaga tgtgcaacac cggcactttc acgcgatcga acttgttctg
      961 ataccgaagc ggctcccacc actcgtccag ttgcgtgtgt tcaaacatct ccttccaata
     1021 ccgattcgga cgtcccgcgg cttcgtccat cgtcttcatc ggcaggtgcc ggtgaatctt
     1081 cggccagtcc acggcgttca tgttttgcga cactcgaccg gccgtgaagt gataccaact
     1141 gatgcccatc ggcaacggca gtccagtagg ccactcaacg aacggatccg acggcgtgac
     1201 catagcaatc atcgtcgtca gatgcggcgg ttgctcgagc gcggtgagcc actgtatgcg
     1261 gccgttgtac gagccaccga tggtgccgat cttccccgtg gaccacggct gcttcgcaca
     1321 ccactcaatc gcgtcgtacc catcgcgccc gtcattacga taaggcacaa actcgccatc
     1381 cgaatcgcca cgcccgcgca cgtccatcgc gacgaagacg taaccacgcg gcacgaaata
     1441 acgggcaatg gccagcgtgc ccgcgctggt cttgatgtac ggcgttcgtg aaaggatcac
     1501 cggaaactgg ccggcactgt ccggtcgata cacatcggca gacaactcaa tgccgtcgcg
     1561 catccgcaca cgctgattga aattgatctt gaccgcatgt gccggttgcg aagcaggcgc
     1621 ctgtgccgca gcgtaaccgt agagcgcaat cgtcagaacg aaagcgaata gaaacgtgat
     1681 cttaaggcgc atagttagtc ttgaggttcg ttgccattcg aacgcttcga ctcgcgatac
     1741 cagcgatggc ccgaagcgta ctcgcgatca gtcggacgca aacgtgacaa cgaacggttt
     1801 ggatcgatgt gggcgcacgc gacgtgaatc gcactgatcg ccgccatcgg agcaacgtag
     1861 gaattcagaa acgaaggact caccaccggc gcaatcaaat acgcatcaca ataacgcgcg
     1921 atcggcgacg tgttgctgtc ggtgatgcca aacgtcggca caccttgttt gcgcgcgcgc
     1981 tggaccgcct caacagtatc tctgagacat tgtccaaaac tgatcgcgat cagcacgtct
     2041 ttcgacgtca acacttcaac tttgtattgc aggttcccac tgctgccgat cagcgcctcg
     2101 gcgtcgaacc caagcgcaag caagccgtag gcaaggtagt atgaaagcga cgatgcaaag
     2161 tccaaaccga ccacgagcac gtgacgcgaa cgtttgatgg tgcgggcgag ttcgatcact
     2221 ttcggcttgt cgagactggt cttcagaatg ttgagattgt cgagcgcttt gtcgagcgcc
     2281 tggtccacgt ggtcggcaac actacgtttt tcgcgtgtcg ctgctttcaa cacagtgtaa
     2341 ggcgtgatgc gggcgacgaa gtgctgacgc aagtccgccg cgaagtcggc aaaagtcttg
     2401 tagccgagaa cctgcgtggt ccgcagaatc gtggtcgcat cgacgcgata acgcttggcc
     2461 atctcgcgcg aggagaggaa acaagtttca tcggcggagt cgaggatggc ccggatcagc
     2521 cgttggcggc gaccgttcag tttcgctcgc gcactggcga aacgtacgtc gagcgcagta
     2581 gtcgccgtct ttgcgtccgc gccgtcgaca cccgagagca caagctccgg cttctttgcc
     2641 ggcgcagctt ttttctttgc cttaggccgt aagttttttg actttgattc gctcaacgtt
     2701 ttctgtgttc gcgctaagat agtctacatc gacctcgaag cccaaccctg aacgctgcgg
     2761 gacatcgacc gtgccgttgg cgttgactgt cacgggagga acaatcacgt cgcgtgcaaa
     2821 at
//
LOCUS       DASTEY010000200         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1372410, whole genome shotgun sequence.
ACCESSION   DASTEY010000200 DASTEY010000000
VERSION     DASTEY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372410"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VFO72_03565"
     CDS             <1..905
                     /locus_tag="VFO72_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9478396.1"
                     /translation="WSLLRLRQIHTNVAQGLVNRIVPSSAFALGVLRSIVHGTFLVSV
                     LLTSFSALGSLPATIMRPTGAMKLLPWGFYDQIQTPSGMLILKTAMGMSLLLSTAGFL
                     TSISTKTSFLLVLFYQGLVRSFGHFNHDEMLAAYYLAVLAFTPCGDAFSLDSRLAKSK
                     RELPRIAYAYPILLMQLLMAWVYFSSALIKLRVAGLKYLSVDNLPTLAIFHSLDNLHD
                     TGFRFAFWLPAIKSYLPFAVGLVILWELLFPLAIFVRRLRWWILGVGMVFHLATLFLM
                     NIFFPHQLAMYLIFVNWDRRSSPD"
     gene            1073..2296
                     /locus_tag="VFO72_03570"
     CDS             1073..2296
                     /locus_tag="VFO72_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9478397.1"
                     /translation="MSKSFRISFVFAGLVTMVLGLAQVAAGQLIISEFRVRGPNGAND
                     EFIEIYNNSGADHTVSALSGSGYGVAASDGTTRCSIPNGTVIPNRGHYLCVNSVGYGL
                     ATYPAGNGTTATGDATYTADIPDNAGIALFNNNSGGASYSLANRLDAVGSTSEANTLY
                     KEGTGYPTLTPFSINYSFYRDDCGKSGSITTFGACPISTPKDTNNNAADFIFVDTNGT
                     SAGAGQRLGAPGPENLSSPIQRNASFTANFLDTCVGGASPPNRVRDFTSDPANNSTFG
                     TLDIRKTIINNTGGNVTRLRFRVIDLTTFPAPSGIADLRPRTSSAVVVTVDRPPCGSG
                     TSNVTVQGTTLEIATPAPPFGQPNGGGFNSSMSAGVVTLATPIANGASVDVRFLLGIQ
                     QTGSFKFYLNIEALP"
     gene            complement(2507..>2992)
                     /locus_tag="VFO72_03575"
     CDS             complement(2507..>2992)
                     /locus_tag="VFO72_03575"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HET9478398.1"
                     /translation="TSHFAGRATRKVVSQPADALLLCRMAWWVSVLSVASRCFSLPRA
                     LAIVAGSEPRATRSHDADVQQRLARLLDQLLATDIFLFRPICWKRAAVLHRYLSRHGI
                     RTRIIFGVRYDAEGKVAGHAWLEADGEPILESTPPDYVVTYRFPSNERFDPQLATVSL
                     E"
BASE COUNT          629 a          867 c          795 g          701 t
ORIGIN      
        1 agtggtcgtt actccgacta cgacaaattc atacaaacgt ggcgcaagga cttgtaaatc
       61 gcatcgttcc ctcgagcgct tttgctctcg gtgtcctgcg ttcaatcgtg cacggcacgt
      121 ttctcgtttc agtgctcctc acttctttct cagcgctcgg atctctcccg gcaacgatta
      181 tgcgtcccac tggcgcgatg aagcttttgc cgtggggttt ctacgaccag attcaaacgc
      241 cgtcgggaat gttgattctc aaaaccgcga tgggcatgtc gttgttgctg agcacggcgg
      301 gttttttgac gagcatatct acaaagactt cgttcttact cgtcctcttt tatcagggtt
      361 tggtgcgaag cttcgggcac ttcaatcacg acgagatgct ggcggcttac tatctcgccg
      421 tacttgcctt tacgccttgc ggcgacgctt tttctctcga tagccggctg gcgaagtcaa
      481 aacgagaact tccgcgcatc gcttacgctt atccgatttt gctgatgcag ttgttaatgg
      541 cgtgggtata cttcagttct gcgttgatca agctgcgagt cgcaggattg aagtacctta
      601 gcgtagacaa cctgccaacc cttgcgatct ttcattctct cgacaacttg cacgacaccg
      661 ggtttcgttt cgcattctgg ctgccggcaa taaagagtta tctgccgttc gcggtagggc
      721 tggtcattct ttgggagttg ctgtttccac tcgcgatctt tgtgcgccgc ctgaggtggt
      781 ggattttggg agttggaatg gtatttcacc tggcgacact ttttttaatg aatatcttct
      841 ttccgcatca gcttgcgatg tatttgatct tcgtaaactg ggaccgcaga tcttcgccgg
      901 attagccttc cgaaggcatt agctaacgcc ttcggcgtag tcatccctgc ggtgttcacc
      961 tcaaactgac ccactacatg gtctacaatt ccgttgactg tcgccaaaaa atcgtgctac
     1021 ggttttccgc cacactgaac atcgtgggcc cacctaaaag gagaatacag gcatgtctaa
     1081 gtcatttcgg atctcgtttg tctttgcggg cctggtaaca atggtgcttg gtctagcgca
     1141 ggttgcggca ggacaactga tcatctctga atttcgcgtc cgtggtccaa acggcgcaaa
     1201 cgacgagttt attgaaatct acaacaacag tggcgccgat catacagttt ccgcactgtc
     1261 gggaagcggt tacggagtcg cagcctctga cggcaccacc agatgctcga ttccaaacgg
     1321 cacggtaatc ccaaaccgcg gccattatct ttgcgtcaac tctgtcggct atggccttgc
     1381 aacctatccg gctggtaacg gcactacggc caccggcgat gctacctata ccgccgacat
     1441 cccagacaac gcgggcatcg ctcttttcaa caacaactca ggcggcgcaa gctactcgct
     1501 cgctaaccgc ttggacgccg ttgggtccac ctcggaagcc aatacacttt acaaggaggg
     1561 cactggatat ccaacgctaa ctccgttctc gattaactat tcgttctatc gtgacgactg
     1621 cggtaaatca ggttcgatca cgacctttgg ggcttgcccg ataagcaccc ccaaagacac
     1681 gaacaataat gctgccgact tcatatttgt tgataccaac ggtacctcag ccggcgcggg
     1741 tcaacgactc ggcgcgcctg gtccggagaa cctgagttca ccgattcagc gaaacgcctc
     1801 tttcactgcc aatttcctcg acacgtgcgt cggcggagca tcaccgccaa atcgtgttcg
     1861 cgacttcacg tcggatccgg cgaacaactc aacgttcggt acgctggata ttcgcaagac
     1921 cattattaac aacacgggcg gtaacgtgac gcgcttgcgt ttccgtgtga ttgatctcac
     1981 gacgttcccg gcgcccagcg gcattgccga cttgcgcccg cgaacgtcga gtgccgtagt
     2041 ggtaaccgta gatcggccgc catgtggcag cggcaccagc aacgtgaccg tacaaggaac
     2101 tacgctcgaa atcgcgaccc cagcgccgcc attcggtcag ccaaacggcg gcggctttaa
     2161 ttcctcgatg tcggccggcg tggtcacact ggcgacaccg atcgctaacg gcgcctccgt
     2221 cgacgtgcgc ttcctgcttg gcattcagca aaccggaagc ttcaagttct atctcaacat
     2281 cgaggcgttg ccgtagcgaa agctgctata cgaagcggaa agaagaggcc cctcagcggc
     2341 ctctttcttt tttgtttgca gcactcgccg aagcccagtc cgctcttccc tgggaccgca
     2401 ggcgtctcgc ctgcaatgag cgcggagcgc gaaaaatgaa aggtgcaggc gggacgcctg
     2461 cggtcccagc atcacggcgt cctgtctgta ccggcatcaa agatttttac tcgagtgaaa
     2521 ccgtagcaag ttgcggatcg aaacgttcgt tcgagggaaa ccggtaagtg accacgtaat
     2581 cgggtggcgt gctctcgagg atcggttcgc cgtcggcttc aagccatgcg tggccggcaa
     2641 ctttcccttc cgcatcgtag cgcacaccga agatgatgcg cgtcctgatg ccgtggcgcg
     2701 aaagatagcg atgaagcacg gcggcgcgtt tccaacagat cggcctgaac aggaaaatgt
     2761 cggtggcgag tagttggtcc agcaaacgtg ccagacgctg ctgcacgtca gcgtcatgcg
     2821 agcgcgttgc acgtggctca gatcccgcga cgattgcgag cgcgcgaggt aacgaaaaac
     2881 agcgcgacgc gactgaaagc acactcaccc accaggccat gcggcataga agcaatgcgt
     2941 cggcaggttg cgacacgacc ttccgcgtcg cgcggcccgc gaagtggctc gt
//
LOCUS       DASTEY010000201         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1405249, whole genome shotgun sequence.
ACCESSION   DASTEY010000201 DASTEY010000000
VERSION     DASTEY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405249"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2674
                     /locus_tag="VFO72_03580"
     CDS             <1..2674
                     /locus_tag="VFO72_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9478399.1"
                     /translation="LTVYEVGEDDGKPYIAMEFVEGETLRQRIEASTLQFKEAIDIAI
                     QIAAGLARAHELGIIHRDLKPENLMLSRDGYAKILDFGLAKLVAERDRALVADSEQKT
                     LMFGVQTESGTLMGTVNYMAPEQLLGQRVDRRCDVFSFGVVLCEMLTGAAPFVHDNRI
                     DTMHAILHRAPQFPGDGHPQLPFELQRILTKALAKTPKERYQTIDELAAELKQIKRNL
                     ELGKSLPVAPTTRLVLKKVAAGARAVDYERELNEAQFKAVTTTEGPLLIVAGAGTGKT
                     RTLVYRVARLVEMGVKPESILLLTFTRRAAASMLTRAAALADARCQRVSGGTFHSLGH
                     SVLRRFADQAGVAKNFTVLDQSDTEDLIDLLRRQIKLTKDRHFPRKRTIAAMFSMMVN
                     KVQSLKQVLNQEYPQFIDERRNLETLYKSFEEFKRARHMLTYDDLLVRLREALETSAT
                     MREQLSEQYRYIMVDEYQDTNKLQAQIVKLMTARHENVAVVGDEFQSIYSFRGASHRN
                     MLEFPKLFPKAQIIKLEENFRSTQPILDVANAIIADVKESYKKRLYSRIDGGQPPVVV
                     SARDENEQSRFVAQRIAELRDEGVPLAEIAVLFRSSSHSFDLEIELGKHGIPFRKFGG
                     MRFAESAHIKDALAFLRVVTKPSDTLSWFRALKLIDHIGDATVYQILEHLGVEQKEFR
                     STRSKAGLFKKLHRFPSRPAYRAQLTRLARVLCTVVESNTPAEQLSAVLRFYRPLLKN
                     KYDDVQRRGRDLEHLQSIAKRYKTSAKLLEDVALDPTELVQGNTTRRSSGFVTLSTVH
                     SAKGLEWDNVFLIWMTDGWFPANRFQDEFDDLEEERRLLYVATTRARKDLHFVYPAVN
                     VYAGPETDGTPTISRFLEPIPPSVLPHASLMGGE"
BASE COUNT          691 a          884 c          848 g          602 t
ORIGIN      
        1 cctgaccgtt tatgaagtgg gcgaagacga cggcaagcct tacatcgcga tggagttcgt
       61 tgaaggtgaa acactgcgtc aacgaatcga agcgagcacc ctgcagttca aagaagcgat
      121 cgacattgcc attcagatcg ccgccggact cgctcgcgct cacgaactcg gcatcattca
      181 tcgcgacctc aaacccgaga acctgatgtt gagccgcgac ggttatgcaa agattctcga
      241 cttcggtctc gcaaaactgg tcgccgaacg ggatcgcgca ctcgttgcgg acagcgaaca
      301 gaagacactc atgttcggcg tccaaactga atcaggcacc ttaatgggca cggtcaacta
      361 catggcgccg gaacagttgt tgggccagcg tgtcgatcgc cgttgcgacg tcttttcgtt
      421 tggcgttgtc ctttgcgaaa tgttgaccgg cgcggcccct ttcgttcacg acaatcgcat
      481 cgacacgatg cacgcgatcc ttcatcgcgc gccgcaattt cccggcgatg gccatccaca
      541 actgccgttt gagctgcaac gaatcctgac gaaggcgctg gcgaagacgc caaaagaacg
      601 ttatcaaacg attgacgaac tcgccgctga gctcaagcag atcaaacgca atctcgaact
      661 cggcaaatca cttccagtcg ctcccacaac gcgtctggtg ctgaaaaaag tggcggcggg
      721 cgcacgtgcc gtcgactacg aacgcgaact gaacgaagcc cagttcaaag cagtgaccac
      781 gaccgaaggc ccgttgctta tcgtcgccgg cgccggcaca ggcaaaacgc gcacgctggt
      841 ttatcgtgtc gcgcgtctgg tcgaaatggg agttaaaccc gagtcaattc tgctcctgac
      901 tttcacacgt cgcgccgccg ccagcatgtt gacgcgcgcg gccgcgctgg cagacgcacg
      961 ttgccagcgg gtaagcggtg gcacgtttca ctcgttgggc cactcggtat tgcgccgctt
     1021 tgccgatcaa gccggtgtgg cgaagaactt caccgtgctg gaccagagcg ataccgaaga
     1081 tctgatcgat ctgctgcgga ggcaaatcaa gctcacaaag gatcgacact ttccacgcaa
     1141 acgcaccatc gcggccatgt tcagcatgat ggtcaacaag gtgcagtcgc tcaaacaagt
     1201 tctgaatcag gagtacccgc agttcatcga cgagcgccgc aatctcgaaa cactctacaa
     1261 gtcgttcgaa gagttcaaac gtgcgcggca catgctgact tacgacgatc tgctcgtgcg
     1321 tcttcgcgaa gcactcgaaa caagcgccac gatgcgcgaa caactctccg agcagtatcg
     1381 ctacatcatg gtcgatgagt accaggacac aaacaagctc caggcgcaga tcgtgaagtt
     1441 gatgactgcg cggcacgaaa acgtcgccgt cgtcggcgat gagtttcagt cgatctattc
     1501 gttccgcggc gcgagccatc gaaacatgct ggagtttccg aagctgtttc cgaaggcgca
     1561 aatcatcaaa ctcgaagaga actttcgcag cacgcaaccg attctcgacg tcgcgaacgc
     1621 gatcatcgcg gatgttaagg agagttataa gaagcggctc tattcgcgca tcgacggcgg
     1681 ccagccaccc gtcgttgtga gtgcgcgcga cgagaatgag caatcgcgct ttgttgcaca
     1741 gcgcattgcg gagctgcgcg acgagggcgt tccgctcgct gagattgccg tactgtttcg
     1801 ttcgagctca cattcgttcg atctggaaat cgaacttggc aaacatggaa ttccgtttcg
     1861 caaatttggc gggatgcggt ttgccgagtc ggcgcacatc aaggacgcgc tcgcgtttct
     1921 gcgggtcgtc acaaagcctt ccgacacgtt gtcgtggttc cgggcgctga agttgatcga
     1981 tcacatcggc gacgcaaccg tgtatcagat cctcgaacat ctcggggtcg aacagaaaga
     2041 gtttcgctcg acgcgcagca aggcgggctt attcaaaaag ctgcatcgct tcccttcccg
     2101 gccggcatac agagcacaac tcacacggct cgcgcgagtg ttatgcacgg tcgtcgaaag
     2161 caacacgccg gccgagcaac tgtcggcggt cctgcgtttt taccggccgt tgttgaagaa
     2221 caaatacgac gacgtgcaac ggcgtggccg cgatctcgaa cacttgcagt cgatcgcgaa
     2281 gcgctacaag acgtctgcga aattgctgga agacgtcgcg ctcgatccca cggaactcgt
     2341 tcagggaaac acgacgcgcc gcagcagcgg ctttgtgacg ctctcgacag ttcactcagc
     2401 gaagggtctc gagtgggaca acgtctttct catctggatg acagacggat ggtttccggc
     2461 aaaccgtttt caggacgagt ttgacgacct cgaagaagaa cggcggctgc tttacgtcgc
     2521 aacgacgcgt gcaaggaagg atctgcattt cgtttatcca gcggtgaacg tttatgccgg
     2581 tccggaaacc gacgggacgc cgactatttc acggttcctg gaaccgattc caccctcggt
     2641 gctgccacac gcgtcgttga tgggcggcga atagtcagtg acagcagcaa gctcgaacac
     2701 gctcttccct gggaccgcag gcgtctcgcc tgctctggtc cgccacacgc cacttacgaa
     2761 gccgtgcagg cgagacgcct cggccccggg aaagaagact tcgcaaccca ggacacgacg
     2821 tgttcaaaaa atttattgac agtggaccag cacgggagta aagcggcgca ctagtcgcag
     2881 tctccccacg ttcacgcgcg acttccctcc ccaaagcctc gcgtaaacga ttacagcttc
     2941 aacgatttct gcgggccatg gtgtctcatc ttgcgctgtt ggtctttgcg gagtatgggc
     3001 gggacctgga agtccttacg gaggt
//
LOCUS       DASTEY010000202         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_169390, whole genome shotgun sequence.
ACCESSION   DASTEY010000202 DASTEY010000000
VERSION     DASTEY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169390"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..721
                     /locus_tag="VFO72_03585"
     CDS             161..721
                     /locus_tag="VFO72_03585"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9478400.1"
                     /translation="MPKISDIEETPNPNAVKFILREPVSNGVARQYGSAEQAENDPLA
                     KSLFDIGHVVSVFYMDRMITIEKDEEGDWDELLPALAVPIRAGEPVSVSAAAIGAAVG
                     GPIAAVTSDDPRLLKINEILDERVRPALMGDGGYLEVLGLTDHTLSIRYQGACGSCPS
                     SLTGTLMAIEGMLKQEVDPELEVVAV"
     gene            814..2742
                     /locus_tag="VFO72_03590"
     CDS             814..2742
                     /locus_tag="VFO72_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478401.1"
                     /translation="MSSLPRIAPVALLLFLCVTLAAAQVPEKPPSTPAKSAADKSEEP
                     KSAEPNPAEAKTPVPLDAERIRIRRSQARSLLIALSTDARAFNDQVLRARSLARIADA
                     LWQVDAEQGRSMFRKAWDAAELADVESHRKLQQEIEQQKSRTGGGYAISLPPNLRREV
                     LKLAARHDRALGEEFLDMLKTQKLEAANAASRQLERLNDAMTQRLGVARELLQSGEME
                     RALQFAEPALTVVGMESISFLTELREKNAAAADERYAALLASSANNPQSHANTVSLLS
                     SYIFTPQLFMIFSDKGVNTSQMSSKIVPADVSAELRNAFFQSAAAILLRPLPPAGQDQ
                     APAGLDAKYLVIKRLLPFFEQSAPAEMVESLRAHLNALNAIVSENTRRRDDDVNRGIR
                     PEKPAEEREQSLLNRIDRAKTSAERDALYIQLASLVAGRGDMRARDYVSKVEDSEVRK
                     QVGAYIDGSLAIQSVQKKKTDQALELVQKGDLTHLQKVWVLTRTAQLLEPTDKQKALD
                     LIETAATEARRIDVSDPSRPQALIAVANALKVVDASRIWEATFDAVQAANSAPGFTGE
                     DGRMVLKFQSKGQSSVYTNSVKEFDLEGIFRELTKDDFERAVELARGFEGEGPRAVAT
                     IAIARAILEPGKRATAKK"
     gene            complement(2798..3544)
                     /gene="bshB1"
                     /locus_tag="VFO72_03595"
     CDS             complement(2798..3544)
                     /gene="bshB1"
                     /locus_tag="VFO72_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HET9478402.1"
                     /translation="MSSDPVQLDVLAVFSHPDDAELSVAGTLLKLKSFGYRTGVCDMT
                     RGEMGTRGTPEIRAQEALEAARLLKLDVRVNLDQPDGHIWPTEVARTSFARVLRTYKP
                     KVLLTTHEDDPHPDHANTSIIVRQAARLASMGRYDEESGLSPVPMPAIMHSLFSRRIV
                     PSFVVDVSGFVEEKMAAIRAYASQFYTADSKEPQTRISEKGFLSEIEVRMGYFGSLIG
                     VEAGEPFYVREALNVDDPLALLTRPMNIYS"
     gene            3616..>4081
                     /locus_tag="VFO72_03600"
     CDS             3616..>4081
                     /locus_tag="VFO72_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478403.1"
                     /translation="MLLVLLLLLQVALPLGAANDPVPDSLLLESGKAAILRTHTSDIR
                     EVKIVSYNIRWRGGEQLKQLAKLLNEDPEIGGAAILGLQEVDRCKKRTGHHNTVKALA
                     DELGLHYAWAAPPAAKPRDEEETGVAIMSVYPLSDVRRIVLPHAGPNRRRRVA"
BASE COUNT          938 a         1144 c         1165 g          834 t
ORIGIN      
        1 ttgtcgcaag ctccaacctc tgggctggaa ttcgataacg ccttcggcgt tcagatcagg
       61 acaccacctg caacgggttg gcgggctttt ctgcgagcct gcgaatcctc agtaaagtcg
      121 ctataatgag tccaaacgag atcgatcagg gagttttgcg atgccgaaga tcagcgatat
      181 tgaagagaca ccaaatccga acgcagtgaa gtttatcctg cgggaacccg tcagcaacgg
      241 cgtcgcaaga caatacggct cggccgagca ggctgaaaac gatccgctcg cgaagtcatt
      301 attcgatatc ggacacgtcg tttccgtctt ttacatggac cggatgatca cgatcgaaaa
      361 ggacgaagag ggcgactggg acgagttact gccggcgctg gcagtcccca tccgagcagg
      421 cgaaccggtc agtgtttctg cggcagcgat cggcgcggct gtaggcggtc caatcgcggc
      481 ggttacgagc gacgatccgc ggttgttgaa gatcaacgag attctggacg agagagtaag
      541 accggcgctg atgggcgacg gcggctatct cgaagtattg ggtttgacgg accacacgct
      601 cagcattcgt taccagggcg cctgcggtag ttgcccgagt tctctaacgg gcacactgat
      661 ggcgatcgaa ggaatgctga agcaggaagt cgatcccgag ttggaggtag tggcggttta
      721 atttttctcc tcagtttctg gaaccgggcg gtgcgtgagg cgtgtggacc gtccggcgtt
      781 tccatcctac attccgtttc aacaggagtt tctatgtctt ccctgccacg cattgctccc
      841 gttgctctgt tactgttttt gtgcgtgact ttggcagccg cccaggtgcc ggagaaacct
      901 ccgagtacgc ccgcgaagtc cgcggccgat aaatccgaag agcctaagtc cgctgagccg
      961 aaccccgcgg aggccaagac tccggtgccg ctcgatgcgg agcgaatcag gatccggcgg
     1021 tcacaggcgc gatcgttgtt aatcgcgctg tccacagacg cgcgcgcctt caacgatcaa
     1081 gttttgcgcg ccagatcgct cgcccgcatc gcggacgcgc tgtggcaggt ggacgccgag
     1141 caggggcgtt cgatgtttcg caaggcgtgg gacgctgccg agcttgccga cgttgagagc
     1201 catcggaaac tccagcagga gatcgaacag caaaaaagca ggactggtgg tggctacgcg
     1261 atctcgctgc cgccgaatct ccggcgcgaa gttttgaaac ttgctgcccg gcacgatcgt
     1321 gccctcggcg aagagtttct cgatatgctg aaaacgcaaa agctggaagc tgcaaatgcg
     1381 gcaagccggc aactggaaag attgaatgac gcaatgaccc aacgtttggg cgtcgcaaga
     1441 gagctacttc agtcgggcga gatggaacgc gcgcttcagt ttgccgaacc ggcgctgacc
     1501 gtcgtcggca tggaatcgat cagctttctt acagagcttc gcgagaagaa cgctgcggcc
     1561 gccgatgaac gttatgctgc actgctcgcc tcgtcggcca ataatccaca atcacacgca
     1621 aacaccgtct cgttgctttc gtcctacatt ttcacgcccc aactgttcat gatcttcagt
     1681 gataaaggtg tcaacacttc gcaaatgtcc tcgaagatag ttccggcgga cgtctcggcg
     1741 gaattgcgaa acgctttctt tcagtcagcg gcggcaatcc tgctgcgacc cctgccaccc
     1801 gcgggacaag atcaagcgcc ggcgggcctt gacgcaaagt atctggtcat aaaacgactg
     1861 cttcccttct tcgagcaatc cgcgccggca gagatggtcg agtcattgcg tgctcatttg
     1921 aatgcgttga acgccatcgt ctctgaaaac acacggcgtc gcgacgatga cgtcaatcgc
     1981 ggcatccgtc ctgagaaacc agccgaagaa cgcgaacagt cactactgaa tcgcattgat
     2041 cgcgcaaaga cctcggccga acgcgatgcg ctttacattc aacttgcctc cctcgttgcc
     2101 ggccgcggcg acatgcgagc gcgcgattac gtaagcaaag tggaagactc agaagtgcgc
     2161 aaacaggtgg gggcttatat cgacgggtcg cttgcgattc aatcggtgca aaagaaaaag
     2221 acggaccagg cgctggagct ggttcaaaaa ggcgacttga ctcacttgca aaaggtctgg
     2281 gtgttgacgc gaactgccca gcttctggag ccaaccgaca aacaaaaagc ccttgacctg
     2341 atagaaaccg ccgccacgga agcacgccgc attgacgtgt cagacccgtc gcggccgcag
     2401 gcgttgattg ccgtggcgaa tgcgttgaag gtggtagatg cgtctcgtat ttgggaagcg
     2461 acgttcgacg ctgtgcaggc agcaaactcc gcgcctggat tcactggaga agacggtcga
     2521 atggtgctga agtttcagag caagggccag tcatcggtct acactaacag tgtgaaggag
     2581 tttgatctcg aaggtatttt ccgcgagctg acgaaagacg attttgagcg cgcagtggaa
     2641 ctcgctcgcg gctttgaagg cgaaggacca cgcgcggtgg caacgatcgc aatcgcacgc
     2701 gcgatactgg aacctgggaa acgcgctacg gcgaagaaat gagctttgtc tttgggcttt
     2761 gagctttgaa acaaagcact aagtacaaag cactaactca agagtaaata ttcattggcc
     2821 gcgtcagcag cgccagcgga tcgtctacgt tcaatgcttc tctaacgtaa aacggctcgc
     2881 ctgcttcgac accaatcaac gatccgaagt aacccatccg cacttcgatc tccgagagaa
     2941 atcctttttc gctgatccgc gtctgcggtt ctttcgagtc agccgtgtaa aactgcgacg
     3001 catacgcccg aatcgccgcc atcttctctt cgacaaaccc cgaaacgtca acgacaaacg
     3061 acggcacgat tcttcgcgaa aacagcgagt gcatgattgc gggcatcggc accggcgaca
     3121 atcctgattc ctcgtcatac cggcccatcg acgccaaacg cgccgcttgt cttacgataa
     3181 tcgacgtgtt cgcatgatcg ggatgcggat cgtcctcgtg cgtggtcagc aacaccttcg
     3241 gcttgtacgt ccgcaacacg cgagcaaagg atgtacgggc cacttcggtg ggccatatgt
     3301 gtccatccgg ttggtcgagg ttcacacgca cgtcgagttt cagaaggcgc gctgcctcca
     3361 gtgcttcctg agcacgaatt tcaggagtac cgcgcgttcc catctctccg cgcgtcatgt
     3421 cacacacgcc cgtgcgataa ccaaacgact tcagtttgag aagtgtcccg gccaccgata
     3481 actccgcgtc gtccgggtgt gaaaaaacgg cgagcacgtc aagctggact gggtctgaac
     3541 tcataggcta gatttagctt accgtctacg tcagtatttt gtcatcgcac ttgagtccga
     3601 caggagaaga aaaccgtgct actcgtccta ctgctcttgc tgcaagttgc gttgccgctg
     3661 ggcgcggcaa acgatcctgt tcctgattcg ctcctgctgg aatcaggcaa agccgcgata
     3721 ctacgcacac acacttcgga cattcgcgaa gttaagatcg tctcctacaa cattcgctgg
     3781 cgcggaggcg aacaacttaa acagctggca aaactcctaa acgaagatcc ggaaattggc
     3841 ggcgcggcca ttctcgggtt gcaggaagta gaccggtgca agaagcgaac gggccatcac
     3901 aacaccgtga aagccctggc tgatgagctg ggcttgcact acgcctgggc ggcgccgccg
     3961 gcggctaaac ctcgagatga agaagaaacg ggcgtcgcga taatgagtgt gtatccgttg
     4021 tcagacgtac gacgaatagt cttacctcac gctggtccga atcgcaggcg tcgcgtcgcg
     4081 t
//
LOCUS       DASTEY010000203         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_54124, whole genome shotgun sequence.
ACCESSION   DASTEY010000203 DASTEY010000000
VERSION     DASTEY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54124"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFO72_03605"
     CDS             complement(<1..398)
                     /locus_tag="VFO72_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478404.1"
                     /translation="MSSNVSIRQIESFSELRAVEEMQKEVWGIPDLDVVPLTHLVAAK
                     AAGGVLLGAFEREMLVGFVYGFVAEEEGEMAHHSHLLAVKHDYRNLNLGYKLKLAQRE
                     EVLAQGINVITWTFDPLQSLNAYFNFTKLGV"
     gene            complement(388..891)
                     /locus_tag="VFO72_03610"
     CDS             complement(388..891)
                     /locus_tag="VFO72_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478405.1"
                     /translation="MLRTNATGPYTRANGRFVAAAGSFVYGPDLDKGPSDLSVEHTFQ
                     LNGLLLLPWQVQISGIFRAQSGFHFSRTGSSADPDGNGTLNGIDLGPGRNAFTAPAYV
                     NLDLRFTKRFDIGERFKVHALFEFFNVLNRQNPAAIETVRSATNFGQLKQVLPGREGQ
                     LGIRIEF"
     gene            complement(899..2308)
                     /locus_tag="VFO72_03615"
     CDS             complement(899..2308)
                     /locus_tag="VFO72_03615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9478406.1"
                     /translation="MKYTSILIALCFGVALPLFGVHSANAQTTTASIEGTVTDPQGRV
                     VAGAQITAKSEALGIERTTTSDDNGFYRLTALPAGTYSLSASHTGFATQTFPNVQLTV
                     NRTVRIEVQLEVGPVQERVSITSETIAIDPTTPATGATVTPRQITEMPVNGRNYLDLL
                     QLVPGVVINHQANAGSDNSTPVLGERSGNNNFMIDGQPNKNTVTGGAAAQFNQETIAE
                     FQVLTAGYKAEFGQGSGAIVNVITRSGGNGFHGVGSLFHRNDAFDASNSLTPGEDAPS
                     LRRYDYSLAVGGPIVRDKVFFFGSGERITERRALNFTFPSTGNAQADRILRDFETPFD
                     NPSRIYETRGFFKLDEQLGRHRLSQEVNYTNGVVKEFLPLSAANSLPSRRNDTSGRSL
                     LLGFSDLVLLGDQGNPWVLTLRGGYRGDRANTAPSHPEAGAGTTFNILSSFTNRQPVR
                     YQCTPLRLFHRDRARSFKC"
     gene            complement(2428..3957)
                     /locus_tag="VFO72_03620"
     CDS             complement(2428..3957)
                     /locus_tag="VFO72_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9478407.1"
                     /translation="MRLQKLFYAIGQLLLVAVLAQVALAQNGSLNGFDDYVHKAMKEW
                     EVPGLAVAIVKGDQVVLAKGYGVRKIGEPAAVDERTLFAIGSSSKAFTAASVALLVDD
                     GKVKWDDPVTKYLPGFEMYDPYVTRELTVRDLLTHRSGLQRGDFLWYGTELDRDEILR
                     RTRFLKPSWSMRSTFGYQNLMFLAAGQLVSRVTGKSWDEFIQQRIFTPLAMSSSSTSI
                     NAFKNADNVATPHAKVNDKVQVIPWRNIDNIAPAGSINSNVVDMAQWVRLQLGQGALQ
                     HQRIFSAAAAKEMHASQTVIRFEPPYSMFYPEAHFLNYGLGWFLSDYRGRKVVEHGGA
                     IDGMRAQVALMPEEKLGLVVLSNMNGSALPVALMYRIFDSFLGTPQRDWSADLLKTAK
                     TLEQQAKAAEKKQEAERVAGTNPSVTLEKYAGTYRNDLYGDVKIGFTNGKLNLRYGPT
                     FTGELEHWHFDTFRTRFFAAGDAKIFVTFALNAQGKLETLVLGLPGMADYPFKRIAEK
                     K"
     gene            4110..>5153
                     /gene="menC"
                     /locus_tag="VFO72_03625"
     CDS             4110..>5153
                     /gene="menC"
                     /locus_tag="VFO72_03625"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9478408.1"
                     /translation="MQTDTAVALPRSQSGLDKFQVEEIEVRTVRLRLNEPFETSFGRI
                     DSRLIVLVSLMGDGLTGWGEVVASEEPRYSYETVGTARHVIREFLAPAILARPLTDLE
                     DLSRRFAPFRGHNMAKAGLELAYMDLVARTHDKSLSQLIGGTRPAVAVGVSLGIQPAL
                     DHLLRRVEEYLALGYQRIKLKIKPGWDIDVVREVRRRHPDILLSVDANSAYRIEDRSH
                     LQQLDEFNLLMIEQPLDHDDLLDHARIQSALRTPICLDESITSVRRARQALDLGSCRI
                     INIKIGRVGGYSEALAIHDLCHARGIPVWCGGMLESGIGRAHNIALASLPGFTLPGDI
                     SASSRYFARDVIVP"
BASE COUNT         1084 a         1423 c         1498 g         1148 t
ORIGIN      
        1 acgccgagct tcgtaaagtt gaaatacgcg ttcaggctct gcaacgggtc aaacgtccag
       61 gtaatcacgt tgatgccctg ggccagcact tcttcccgtt gcgcgagctt tagtttgtaa
      121 ccgagattca ggttgcggta gtcgtgtttg accgcgagca agtgcgagtg atgggccatc
      181 tcgccctctt cttcggccac gaaaccgtag acaaaaccta ccagcatttc gcgctcgaac
      241 gcgccgagta gtacgccgcc ggcagctttc gccgcgacga ggtgcgttag cgggacaacg
      301 tcgagatcgg gaatacccca gacctctttc tgcatctcct caaccgcgcg cagctcgctg
      361 aaactctcaa tctgtcgaat ggaaacgtta gaactcaatt cgtatcccga gttgtccttc
      421 acgtccgggc agcacctgtt tcaattggcc gaagtttgtt gccgaacgaa cagtctcgat
      481 ggccgccggg ttctggcgat tcagcacatt gaagaactcg aagagcgcgt gcaccttgaa
      541 tcgttctcca atgtcaaaac gcttggtgaa gcgcaggtcg agattgacat atgccggcgc
      601 cgtgaaggca ttccggcccg gaccaagatc gatgccgtta agagtaccat tcccgtcggg
      661 atccgcagag gagccggtgc ggctgaaatg aaatccgctt tgcgcgcgaa agatgccgct
      721 gatctgcacc tgccacggca gcaacagcaa accgttcagt tggaaagtgt gctctactga
      781 aagatcagac gggcctttgt ccagatcggg accgtaaaca aacgatcccg cagccgcaac
      841 aaaccgcccg ttagcccgcg tgtaaggacc agtcgcattt gttcgcagca cgccgccttc
      901 aacacttgaa actacgggcg cgatcccgat gaaacagacg gaggggagtg cattgatacc
      961 taactggttg tcggttagtg aacgaactta agatattgaa cgtcgtgccg gcgcctgcct
     1021 ccggatgtga cggcgccgta ttggccctat cgccacgata accaccgcgc agcgtcaaca
     1081 cccatggatt cccctgatcg cccagtagaa cgagatcgct gaacccgagt agcaagctgc
     1141 gcccgctcgt gtcgtttcta cgcgacggta aactgtttgc cgccgagagc ggcagaaact
     1201 ccttgacgac gccattcgtg taattcactt cctgcgagag acgatggcgt ccgagttgtt
     1261 cgtcgagttt gaagaagccg cgtgtttcgt agatacgcga aggattgtca aaaggcgttt
     1321 cgaagtcgcg caggatccgg tcggcttgag cgttgcccgt gctcgggaaa gtaaaattta
     1381 acgcacgcct ttcggtaatg cgttctcccg aaccgaagaa gaagactttg tctctgacta
     1441 ttggaccacc gaccgcgagg ctgtagtcat aacgtcgcag cgatggcgcg tcctcgcctg
     1501 gcgtgagcga gttggacgcg tcgaaagcat cgttgcgatg aaagagggac ccgactccgt
     1561 gaaaaccatt tccgccgctg cgcgtgatga cgttcacgat cgcgcctgag ccctgtccga
     1621 attccgcttt gtaaccggca gtcagcacct gaaactcggc aatcgtctct tgattgaact
     1681 gcgccgcggc gccgccggtg acggtgttct tgttcggctg gccgtcaatc atgaagttgt
     1741 tgttgcccga acgctccccc agcaccggcg tggagttgtc ggagccggca tttgcctgat
     1801 gattgatcac aacgccggga acgagttgta acagatcgag ataatttcga ccgttgaccg
     1861 gcatctccgt gatctggcgc ggtgtgacag ttgcgcctgt ggccggtgtc gtcgggtcga
     1921 ttgcgatcgt ctcggaagtt atgctcacgc gttcctgcac cggaccaact tccagttgca
     1981 cctcgatcct gacggtgcga ttaacagtca gttggacgtt tggaaaagtt tgcgtggcga
     2041 aaccggtgtg cgacgcggag agggagtaag ttcccgcggg cagcgcagtg agacgataga
     2101 agccgttgtc gtcactcgtt gtcgtgcgtt cgatgccgag cgcctccgac ttcgctgtga
     2161 tttgcgcacc agcgacaacg cgaccctgcg gatcggttac cgtgccctcg atggaggcgg
     2221 tcgtggtctg cgcgttcgcc gaatggaccc cgaaaagagg aagggcaact ccgaaacaga
     2281 gcgcgatcaa aatggatgtg tatttcatca ctactttgcc tccttcgaga agttgcttcc
     2341 tgggaccgca ggcgtatcgc ctgcgactaa cgattgaggt ctctttcggt gcacggcagg
     2401 cgagacgcct gcggttccag gaaatagtta tttcttttcg gcaatgcgtt tgaacggata
     2461 gtcggccatg cccggcagac cgagtactaa tgtttcgagc ttgccctgag cgttaagtgc
     2521 aaacgtgacg aagatcttcg cgtctccggc ggcgaagaaa cgcgtccgaa aagtgtcgaa
     2581 gtgccagtgt tcgagttctc cggtgaaggt cggtccatac cgcaggttca acttgccgtt
     2641 ggtgaaccct attttcacgt caccgtagag atcgtttcgg taagtgccgg catatttttc
     2701 cagcgttact gaaggattcg tgccggccac gcgctccgct tcctgcttct tctccgcggc
     2761 tttggcttgc tgttcgagtg tcttcgcagt cttcagcaag tcagcgctcc aatcgcgttg
     2821 cggcgtgccc agaaaagaat cgaagatgcg atacatcagc gccacgggca gtgctgagcc
     2881 gttcatgttg ctgagaacga ccaggccaag tttctcttcg ggcattaatg cgacctgcgc
     2941 tcgcatgccg tcgatggcgc cgccatgttc gacgaccttg cggccgcggt agtcggaaag
     3001 aaaccaaccc agaccgtaat tgaggaagtg cgcttccgga tagaacatcg agtacggcgg
     3061 ttcgaaacga atgacagtct gcgacgcgtg catctccttt gcggcagcgg cactgaagat
     3121 tcgttgatgc tggagagcgc cctggcccaa ttgcagccgt acccactgcg ccatgtcgac
     3181 gacgtttgaa ttaatcgaac cggccggagc gatattgtcg atgttgcgcc agggaattac
     3241 ctgcactttg tcattgactt tggcgtgtgg agtcgcaacg ttgtcggcgt ttttgaaagc
     3301 gttgatgctg gtgctgctcg acgacatcgc gagcggcgta aagatgcgtt gctggatgaa
     3361 ctcgtcccag ctcttgccgg tgactcgcga cacgagctgg ccggcggcga gaaacatcag
     3421 gttttgatag ccaaatgtgc tgcgcatgct ccacgacggt ttcaaaaagc gcgtgcgccg
     3481 caagatctcg tcgcgatcga gttccgtgcc gtaccagaga aaatcaccgc gttgcagccc
     3541 gctgcgatga gtcagcagat cgcggacggt cagttcgcgc gtgacgtacg ggtcgtacat
     3601 ctcgaagccc ggcaggtatt tcgtgaccgg gtcgtcccat ttcactttgc cgtcatcgac
     3661 cagcagggcg actgaagcgg ccgtaaacgc cttcgacgac gacccaatag cgaagagcgt
     3721 gcgctcgtcg acagcagcgg gttcgccgat ttttcgtacg ccataacctt tggcgagtac
     3781 gacctgatcg cccttgacga tggcgacagc gaggccgggg acttcccact ctttcatcgc
     3841 cttgtggacg tagtcgtcaa atccattcag cgatccattt tgcgccagag cgacttgagc
     3901 aagtactgcg accagcagca actgcccgat tgcataaaag agtttttgta gtctcataga
     3961 cgggagccgc tttcaaaaac ggagagacat tattttcagt tggaaaagtt gggcgttata
     4021 taccgcacat gctgcattct gtcaatacac gcgacaagtg ctgcaaatcg tattgagttc
     4081 tacggcgcga tttggtattg tgcgcggcca tgcaaactga cactgcggtt gcgttgccac
     4141 gttctcaatc aggcctcgat aagtttcagg tcgaggagat tgaagtgcga acagttaggt
     4201 tacgcctcaa cgagccgttc gaaacaagct tcgggcgaat cgactcgaga ttgatcgtgt
     4261 tggtcagtct catgggcgat ggcctgaccg gatgggggga ggtggttgcc tccgaagagc
     4321 cgcgttacag ctacgaaacc gtgggcactg cccgacatgt gattcgcgaa ttccttgcgc
     4381 ccgcaattct ggctcggccg ctgactgatc tcgaggatct cagccggcgg tttgctccgt
     4441 ttcgcggtca caacatggcg aaagcgggac tggagctcgc ttacatggat ctagtcgcgc
     4501 ggacgcacga caaatcgctg tcgcagttga ttggtggaac gcgtccggca gttgcggtgg
     4561 gagtgagtct cggaattcag ccggcgctcg atcacttatt gcggcgcgtc gaggagtatc
     4621 tcgcgcttgg ttatcagcga atcaaactca aaatcaaacc gggttgggac attgatgtcg
     4681 tgcgcgaagt gcggcgacgt catccggaca ttctgctctc agtcgatgca aactcggctt
     4741 atcgcattga ggaccgctcg cacttgcagc agcttgacga attcaaccta ctgatgatcg
     4801 agcaaccact cgatcacgac gacctcttag atcatgcccg gattcagtcg gcgctgagga
     4861 cgccaatctg tctggatgaa agcatcacga gcgtaaggag agcgagacag gcgctcgatc
     4921 tcggcagttg ccggatcatc aacatcaaga tcggacgcgt cggcggctat tccgaagccc
     4981 ttgcgataca cgatctttgt cacgcgagag gaattcctgt gtggtgcggc ggcatgctcg
     5041 agtcaggcat cgggcgcgcg cacaacatcg cgctcgcgag tcttccgggc ttcacactgc
     5101 cgggcgacat ctcggccagt tcgcgttatt ttgcacgcga cgtgattgtt cct
//
LOCUS       DASTEY010000204         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_482762, whole genome shotgun sequence.
ACCESSION   DASTEY010000204 DASTEY010000000
VERSION     DASTEY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482762"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VFO72_03630"
     CDS             complement(<1..268)
                     /locus_tag="VFO72_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478409.1"
                     /translation="MAPYPNLVSDSTLVQDTIDLLNDFGGRAPASEIVDAVFKLSHID
                     DELAGLLVADLVRNDRRFKISDNTVELLQDDSHSRLLKDLDFVVV"
     gene            315..893
                     /gene="pyrE"
                     /locus_tag="VFO72_03635"
     CDS             315..893
                     /gene="pyrE"
                     /locus_tag="VFO72_03635"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9478410.1"
                     /translation="MDPEKVIDEFKKTGALLEGHFQLSSGLHSTVYLQCALVLQFPEK
                     AETFGRAIGERFADAGIQLVASPAIGGIVIGHEVARALGARFVWTEREGGAMTLRRGF
                     SVSRGERTLIVEDVITTGGSTRETVEALQRAGADVVAAASIIDRSGGGAEVGVPRMAL
                     APLKVLSVEPEICDACKTGEPVVKPGSRSANP"
     gene            890..1645
                     /gene="truA"
                     /locus_tag="VFO72_03640"
     CDS             890..1645
                     /gene="truA"
                     /locus_tag="VFO72_03640"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9478411.1"
                     /translation="MNYKLLLQYDGTDFHGWQSQDGLRTVQGELEQALTLIEGKRVNV
                     FGSGRTDAGVHAEGQVANVELQREISAQKLRSAINGNVGKDVRVLEAEEVSSEFHARY
                     SALEKTYVYRIVNAPVMSPFWSRFAHHEARPLNLSQMQTAAELFLGKHDWTAFSAAQS
                     DVEDRVRTITNLEISERLDERSHGTLVQIRVSADGFLRYMVRTIAGTLMAAGRGELDR
                     SAVAQAIETGVRPIFSTTAPACGLTLLSVRYTD"
     gene            1683..2474
                     /locus_tag="VFO72_03645"
     CDS             1683..2474
                     /locus_tag="VFO72_03645"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET9478412.1"
                     /translation="MLSDFSGVVQKGSRDEALLAAIAWDRLPRHVAIIMDGNGRWAAQ
                     RGQPRIAGHRAGVEAVRAAVDTGARLGLSALTLYAFSTENWKRPRYEVDALMRMLRRY
                     IRLELDEIDRQNIKFQTIGRIGALAENVRKEIERATERTARNKGMILTVALNYGGRSE
                     IVDATRAAVRRLMDEGSRPDELTEERIANELYTRNLPELDLLVRTSGELRISNFLLWQ
                     SAYSEIYVTETLWPDFRRVHLLEAVVDYQRRDRRFGGLKLVVAGK"
     gene            2475..>3302
                     /locus_tag="VFO72_03650"
     CDS             2475..>3302
                     /locus_tag="VFO72_03650"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9478413.1"
                     /translation="MQQTTVKKPEAKSSGSARLITAAVVLPFLIASILLPWLKWLFVA
                     MAITAMSLALFEFYVLAKQRGLQPDTGAGFLAAIAILVISVFPADLYNVLFVLLVVMA
                     LTAGTLVAATLRGAPFDKMLGSAGATILGVLYIAMLGSHLVSLRMWDREALAADLLSF
                     FFLVLMGADAGAYYLGRALGKHKLAPSISPGKTWEGVVGGVAAAMVMAAVAHYWFFRE
                     LPLKYALPLAVVMTVVGIFGDLTESALKRGAGAKDAANILPGHGGLLDRLDSLLFNAP
                     "
BASE COUNT          672 a          848 c         1030 g          752 t
ORIGIN      
        1 cgacaactac gaaatccaga tccttgagca aacgtgaatg gctgtcgtct tggagcagtt
       61 cgacggtgtt gtcactgatt ttgaaacgac gatcgtttct gaccagatct gcgacgagca
      121 ggcccgcgag ttcatcgtcg atgtgtgaaa gtttgaaaac ggcgtcgaca atttcagaag
      181 cgggcgcgcg tccgccgaaa tcgtttaaga ggtcgattgt gtcctgaacg agtgtggagt
      241 cggaaactaa atttggataa ggcgccattc ggcgttggat tgtagcacgg cggcgaagtt
      301 tatgaggtga caatttggac cctgaaaagg taatcgacga gttcaagaag acgggtgcac
      361 tgctggaggg ccactttcaa ctaagcagcg gactgcacag cacggtctac cttcaatgtg
      421 cgttggtgct ccagtttccg gagaaggcgg aaactttcgg acgcgcgatt ggagagaggt
      481 ttgccgatgc gggaattcag ctggtggcat cgccggcgat tggtggaatt gtgattggtc
      541 atgaagtggc gcgcgcgttg ggtgcgaggt ttgtgtggac tgaacgcgaa ggcggcgcga
      601 tgacgttgcg gcgtgggttt agtgtctcgc gtggtgaaag aacgctgatc gttgaagacg
      661 taattacaac gggtggttcg acgcgcgaga ccgtcgaggc gttgcagcgt gcaggtgctg
      721 atgtggttgc ggcggcgtcg atcatcgacc gctcgggtgg cggcgctgag gttggggtgc
      781 cgcggatggc tctcgccccg ttgaaagtgt tgtcggttga gccggaaatc tgtgatgcgt
      841 gcaagacggg agagccagta gtgaagccag gcagccgcag cgctaatcca tgaactacaa
      901 actcctactc cagtacgacg gcacggattt tcacggctgg cagagccagg acggattgag
      961 aacggttcag ggcgagttgg agcaggcgct gacgttaatt gagggaaagc gcgtgaacgt
     1021 ctttgggtcg ggccgcacag atgctggagt tcatgctgaa ggccaggtcg cgaacgttga
     1081 gctccagcgc gagatatccg ctcagaagct gcgctcggca atcaacggaa acgtcggtaa
     1141 agacgtgcgc gtgctcgagg cggaagaagt cagcagtgag tttcacgcgc gatattcggc
     1201 gctcgagaag acctacgttt accgcatagt gaatgcgccg gtgatgtcgc cgttctggtc
     1261 gcgctttgca catcacgaag cgcgcccgct caacctttca caaatgcaaa cagcagcgga
     1321 actgtttctc ggcaaacacg attggaccgc cttttccgca gcgcaatcag acgttgaaga
     1381 tcgcgtccga acgattacta atctggagat cagtgaaaga ctggacgaac gctcgcacgg
     1441 cacgttggtc cagatccgcg tgagcgcgga tggttttctt cgctacatgg tgcggacgat
     1501 tgcgggcacc ttgatggcgg ccggccgcgg cgaacttgac cgttctgcag tcgcacaagc
     1561 aatcgaaaca ggggtgcgcc caattttctc gactaccgcc cccgcctgtg gccttacgtt
     1621 gctgtcggtg aggtacacgg attgacagcg ctcgtcttca tgatcaagag tctcgttctc
     1681 gtatgctttc ggatttcagt ggcgtagttc aaaaaggatc gcgtgacgag gcgctgctcg
     1741 ccgcgatcgc ctgggaccgc ttgccgcggc acgtcgccat cattatggat ggcaacggcc
     1801 gttgggccgc gcaacgtggt cagccgcgaa tcgccggaca tagagcaggt gttgaagcag
     1861 ttcgcgccgc cgtcgacacc ggtgcgcgtc tcggactcag cgcgctgacg ttgtacgcat
     1921 tctccaccga gaactggaag cgtcctcgct acgaagtcga cgccctgatg cgcatgctgc
     1981 gtcgttacat acgtctcgaa ctcgacgaga tcgaccggca gaacatcaag tttcagacca
     2041 ttggccgcat tggcgctctt gcggaaaacg tccgcaaaga gatcgagcgt gcaactgagc
     2101 gcaccgctcg aaacaagggc atgatcttaa cggttgcact caactatggc gggcgttccg
     2161 agattgtcga tgcaactcgc gcggccgtgc ggcgtttaat ggacgaaggc agccgtcctg
     2221 acgaactgac cgaagaacga atcgcgaacg agctctacac acgaaacttg ccggagttgg
     2281 atttgctggt gcgtaccagc ggcgagttgc gtatctcaaa ctttctcctg tggcaaagtg
     2341 cttatagcga gatctacgtc acagaaactt tgtggcctga cttccgccgc gttcatctgc
     2401 tcgaagctgt tgttgattat caacggcgag accggcgctt tggcggcttg aagctggttg
     2461 tggccggaaa ataattgcag caaactaccg tcaagaaacc agaagcaaag agttccggct
     2521 ccgcacgtct aatcaccgcc gctgttgttc ttccttttct tatcgcttcg attttacttc
     2581 cgtggctcaa gtggctgttc gttgcgatgg caattactgc gatgtcgctg gcgctgtttg
     2641 agttctacgt actcgcgaaa cagcgcggtt tgcagccgga taccggcgcc gggttcttag
     2701 ccgcaatcgc gattctcgtc atttctgttt tccccgccga tctgtataac gtgctgttcg
     2761 tgcttttggt agtcatggcc ttgaccgccg gcacgctagt cgccgccacg cttcgtggcg
     2821 cgcctttcga caaaatgctc ggttcagcag gcgctacgat tctgggcgta ctttacattg
     2881 cgatgctggg cagtcatctc gtctctctgc gcatgtggga ccgagaagct ttggccgcag
     2941 atctgttgtc gttctttttc ctggtgttaa tgggcgccga tgccggcgcg tactaccttg
     3001 ggcgggcgtt gggaaaacac aagctggcgc catcgatcag tcccggtaag acgtgggaag
     3061 gtgttgttgg aggtgttgcg gccgcaatgg tgatggccgc tgtggctcat tactggttct
     3121 ttcgtgagtt gccgttgaag tatgcgctgc cgctggcagt tgtgatgacc gtggttggaa
     3181 tcttcggcga cctaacagag agtgcattga aacgaggcgc gggcgctaaa gacgccgcca
     3241 acattttgcc cggacacggt ggcctactcg accgtttgga tagcttgctc ttcaacgcac
     3301 cg
//
LOCUS       DASTEY010000205         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_927354, whole genome shotgun sequence.
ACCESSION   DASTEY010000205 DASTEY010000000
VERSION     DASTEY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927354"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1274
                     /locus_tag="VFO72_03655"
     CDS             60..1274
                     /locus_tag="VFO72_03655"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET9478414.1"
                     /translation="MTLTLEGNRAKSSAQQVQLGLERLLEDHAPLLERARVGLICNQA
                     SVDHGYRHAADLLYEHPSIDLRALFGPQHGIRGDVQDNMIETAHGVDRKTTLPVHSLY
                     SETREPTEAMLNDVDVLVFDMQDVGCRIYTFVYTLANCMRAAARFGKRVVVCDRPNPI
                     NGVSVAGNILESEYASFVGQFPLPTRHGMTVGELARMFNEHFKIGCDLEVVSMNGWRR
                     EFWHDDTDTPWVLPSPNMPTLDSATVFPGTVHFEGTQVSEGRGTTRPFELIGAPYIDP
                     DEYGDKLNALNLPGVFFRSCAFQPTFQKHAGVTCGGVQIHLTNRDVFEPVFAGIAMVK
                     LIYDMYPNEFRWKEPPYEYVYDKNPFDVIAGTNKIREAFEQGDKLDAIAETWTRPLLE
                     FKQLRESFLIYG"
     gene            1497..1931
                     /locus_tag="VFO72_03660"
     CDS             1497..1931
                     /locus_tag="VFO72_03660"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HET9478415.1"
                     /translation="MVKSERLQVEHVEPTPGSAWLVGISAWFIPGSGHLILHKWGRAA
                     LMGGVVWLCFFLGLAMGGHMFDLSTGQGSSVWLQVPPMIANMGSGALFIISWLLNIGF
                     EDDPVQAARATYEYGNTFLLIAGLLNYLTMLDAFDIAAGRRS"
     gene            1928..2098
                     /locus_tag="VFO72_03665"
     CDS             1928..2098
                     /locus_tag="VFO72_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478416.1"
                     /translation="MRHFLVMCLFAAIASVVFGAMAKDTNRERWLYGVKIFAEFVVIG
                     LMLAWILYFIPW"
     gene            2102..2839
                     /gene="recO"
                     /locus_tag="VFO72_03670"
     CDS             2102..2839
                     /gene="recO"
                     /locus_tag="VFO72_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9478417.1"
                     /translation="MAVFETEALVLRTYNLAEADKIVVCLSRSAGLIRGVAKGCRKLK
                     NRFGASLEPFTLINLTYYEKEHQELVSFRQTEILRSRFNLSGNASILTGFSYMGDLLI
                     DFSPPHQANDNLYRMALACFEAASQSPEDLESVLRYFEVWLLKIEGFLPDLRACANCH
                     RPFSEETIYLGNGLTLRCGQCSNGRGGVVSKRLHSQLRATEKLTPAKFAEESREVSSA
                     TKREMAELTFQLIGRVLERMPRIRPNV"
BASE COUNT          655 a          748 c          844 g          726 t
ORIGIN      
        1 taaacgtcaa cggtggatcg gtcttgtgcg gttaacagtt caagaatggg acttcaagaa
       61 tgaccttaac tctcgaagga aatagagcaa agagttcagc gcaacaggtg cagctgggac
      121 ttgagagatt acttgaagat catgccccac tgctcgagcg ggcgcgtgtt gggttgatct
      181 gcaaccaagc gtcggtcgat cacggctatc ggcacgcggc ggacctgctt tatgaacatc
      241 cttcgatcga tctgcgcgcg ctgttcggac cgcaacacgg cattcgtggc gacgtgcagg
      301 acaatatgat cgaaacggcg cacggcgtcg atcgcaagac tactctgccg gttcattcgc
      361 tctacagcga aacaagagaa ccaaccgaag cgatgttgaa cgacgtcgac gtgctcgtgt
      421 ttgacatgca ggacgttggt tgccgcatct atacctttgt ctacacgctg gcgaactgca
      481 tgcgagccgc cgcacgtttc gggaagagag tagtggtttg cgatcgtccg aatcccatca
      541 acggtgtcag cgtcgcggga aacatactcg aatcggagta cgcttcgttt gtgggccagt
      601 ttccattgcc tactcgccac ggtatgacgg tcggcgaact agcgcgaatg ttcaatgaac
      661 acttcaaaat cggctgcgat ctcgaggttg tatcgatgaa tggctggcgc cgggagttct
      721 ggcacgacga caccgacaca ccgtgggttt tgccttcgcc aaacatgccg acgctggatt
      781 cagcaacagt gtttccgggc acggtccatt tcgaaggcac gcaagtctcg gaaggtcgtg
      841 gaacgacgag gccgttcgaa ttaattggcg cgccgtacat cgatcccgac gaatacggtg
      901 acaaactaaa cgcgctcaat ttgccgggcg tgttcttcag aagctgcgcg tttcaaccaa
      961 catttcagaa gcatgcaggt gttacttgcg gcggcgtgca aattcacctg acgaatcgcg
     1021 acgtgtttga acctgtattc gccggcatcg cgatggtaaa gctgatttac gacatgtacc
     1081 cgaacgagtt tcgctggaaa gagccgcctt acgaatacgt ctacgacaag aatcctttcg
     1141 acgtgatcgc gggcacgaac aagattcgcg aagcgttcga gcagggggac aaactcgatg
     1201 cgattgcgga gacgtggacg cggccgctgc tcgagttcaa gcaactgcgc gaatcgtttt
     1261 tgatctatgg atagctaagg tagatcgtga gaggtcgcca cgccgaccgc taaggttcag
     1321 catgaagact tctcgcgcaa aggcgccaag acgcaaaggc gttcaagtta ctcttgaagt
     1381 gcgctcttct tcaaaaggag ttttccttgc cgctcttctt tgcgacttgg cgcctttgcg
     1441 cgagactctt tttctaaggt aaaagattag acgcgcattt aggatggagg agtgctttgg
     1501 ttaagtcgga acggttacaa gtcgagcacg ttgaaccgac gccgggttcg gcatggctcg
     1561 tcggcatttc tgcgtggttc attcccggca gcggacatct gatactccac aagtggggcc
     1621 gggcggcgct gatgggtggt gttgtttggc tctgcttctt cttaggcctt gcgatgggcg
     1681 gacacatgtt tgatctttcc accgggcaag gttcatcagt gtggcttcag gtgccgccta
     1741 tgattgcgaa catgggcagc ggcgcgctct tcattatttc gtggctatta aacattggct
     1801 tcgaagatga tcctgtacaa gcagcgcgag ccacttacga atacggcaac acgtttcttc
     1861 tgatcgccgg cttattgaat tacttaacga tgctcgatgc gtttgatatc gcggcaggga
     1921 ggcgatcgtg agacatttcc tggtgatgtg cctgttcgcc gcgatcgctt ccgtggtgtt
     1981 tggcgcgatg gctaaagata cgaaccgcga gcgctggctt tacggagtca agatttttgc
     2041 cgaattcgtg gtgattggtc tgatgttggc ctggatcctc tactttattc cgtggtgaga
     2101 tgtggccgta tttgaaaccg aggccctggt cctgcgcacg tacaatctgg cagaggctga
     2161 caagattgtc gtgtgcttaa gccgttcggc gggcctgatt cgcggggttg cgaagggttg
     2221 tcgcaaactc aagaaccgct ttggagcttc gctcgaacca ttcacgctca ttaacctcac
     2281 gtattacgag aaggaacacc aggaactcgt ttcgtttcgt caaaccgaaa tcctgagatc
     2341 tcgctttaac ctttccggca atgcgtcgat tctcaccggc ttctcttaca tgggcgatct
     2401 gctgatcgac ttttcgccac cacaccaggc aaacgacaat ctctatcgca tggcgctcgc
     2461 atgtttcgaa gcagcatctc aatcgcccga agatctcgaa tcggtgttac gttacttcga
     2521 agtgtggctg ctgaagatcg aagggttttt gcctgacttg cgcgcttgcg caaactgtca
     2581 tcgtcctttt agtgaagaga cgatctattt aggcaacggt cttacactac ggtgtggtca
     2641 gtgcagcaac ggtcggggtg gcgtcgtgtc caaacgtttg cattcgcagt taagagctac
     2701 agaaaaacta acaccggcga agttcgcgga agagtcgagg gaagtttcga gtgccacaaa
     2761 gagagagatg gcggagttga cgtttcaact gatcggccgc gttctggaac gcatgccgcg
     2821 aatacgacct aacgtataag tgttaaagta tcgccttaga tccacgacct tgctgatctc
     2881 gcgctacatc tttggcgcga tggcgccata catcctgctc gcgttgctga tgttgagtgc
     2941 ggtgctgttt gcgcaacagg cgagccgtct tac
//
LOCUS       DASTEY010000206         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1355994, whole genome shotgun sequence.
ACCESSION   DASTEY010000206 DASTEY010000000
VERSION     DASTEY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355994"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..845)
                     /gene="rplC"
                     /locus_tag="VFO72_03675"
     CDS             complement(189..845)
                     /gene="rplC"
                     /locus_tag="VFO72_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET9478418.1"
                     /translation="MINGIIGRKIGMTQIFAPDGSVTPVTVIKAGPCVVVQKKSASGP
                     DGYDAVQLGLVDDRPQRLKNVTKPMRGHFEKTGGGIPPTRILKEFRLLESGDGTNVGD
                     KVLVDQFSDGEMVDVIGRSKGRGFAGTIKRHHFNRGPESHGSMNVRAPGSIGASAYPS
                     RVVKGNRSSGHMGDARITVKGLTVARVDAENNILMIRGAVPGANGSVVVVRKATKKTK
                     "
     gene            complement(887..1204)
                     /gene="rpsJ"
                     /locus_tag="VFO72_03680"
     CDS             complement(887..1204)
                     /gene="rpsJ"
                     /locus_tag="VFO72_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET9478419.1"
                     /translation="MNEKIRIRLKAYDHRVLDQSTTEIVETAKRTGARVAGPIPLPTV
                     KNKWTVLRSPHVDKKSREQFEIRTHKRLMDILDPTPQTVDALMKLDLPAGVDVEIKAF
                     GRQ"
     gene            complement(1340..2527)
                     /gene="tuf"
                     /locus_tag="VFO72_03685"
     CDS             complement(1340..2527)
                     /gene="tuf"
                     /locus_tag="VFO72_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9478420.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAKNNPKVQ
                     FVAFDQIDKAPEEKARGITIATAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAMVVCLNKVDAVDDPELLELVELEVRELLG
                     AYEFPGDDIPVIKCSALKALEGDPEWEKTVLELMDAVDSYIPTPERDIEKPFLMPVED
                     IFTIQGRGTVATGRVERGKVKVNEAVEIVGIRPTTKTVVTGVEMFKKLLDEGVAGDNV
                     GLLLRGTERKDIERGQVIAKPGSITPHTKFKAEAYVLTKEEGGRHTPFFTGYRPQFYF
                     RTTDVTGVAHLPEGVEMVMPGDNVAMEIELIAPIAMEKGLRFAIREGGRTVGAGTVSD
                     VVE"
     gene            complement(2580..2654)
                     /locus_tag="VFO72_03690"
     tRNA            complement(2580..2654)
                     /locus_tag="VFO72_03690"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2620..2622),aa:Thr,seq:ggt)
     gene            complement(2679..2754)
                     /locus_tag="VFO72_03695"
     tRNA            complement(2679..2754)
                     /locus_tag="VFO72_03695"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2719..2721),aa:Gly,seq:tcc)
     gene            complement(2849..2936)
                     /locus_tag="VFO72_03700"
     tRNA            complement(2849..2936)
                     /locus_tag="VFO72_03700"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2900..2902),aa:Tyr,seq:gta)
     gene            complement(2954..3029)
                     /locus_tag="VFO72_03705"
     tRNA            complement(2954..3029)
                     /locus_tag="VFO72_03705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2994..2996),aa:Thr,seq:tgt)
     gene            3218..>3664
                     /locus_tag="VFO72_03710"
     CDS             3218..>3664
                     /locus_tag="VFO72_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478421.1"
                     /translation="MATFRFSRALLLLPILFLLALAGTAGAQTPEVVAPTAKEPMHVA
                     GKTQLYCAGYVRLQRLPNMPQVVGAEEEQEQRMFTDGDVVYINAGSRQGIADGQNFQI
                     IRPRGDIKGVYRQKPGFLGTYIQEVGQLQVLKVGENTSAAQITSSCG"
BASE COUNT          759 a         1208 c          873 g          824 t
ORIGIN      
        1 gcgccggaaa catcgccacg agtcttcgtc gcggaagtgc cctgacgcgc gttggccatg
       61 taattccgca cggccgcgtg aatcaaacct tcgtttagag gcgcgccaaa aacagcgtca
      121 gacagctcga cctcaccaac ctcttcgttc ttgatattgc gcaccttgac tgtcggcatc
      181 actaactcct acttcgtctt cttcgtcgcc ttgcggacaa ccactacgct gccgttagct
      241 ccaggcaccg cgccacgaat catcaaaatg ttgttttcgg catcgacgcg cgcgactgtc
      301 agccccttca cggtgatgcg agcatcgccc atgtggcccg acgacctgtt tcccttgact
      361 actcgcgacg gatacgccga cgcaccaatc gaacccggcg cccgcacgtt catcgaaccg
      421 tgcgactccg gaccacgatt gaaatggtga cgcttgatcg tgcccgcgaa accgcgaccc
      481 tttgaacgcc cgatcacgtc caccatctcg ccgtcagaaa actgatcgac aagcactttg
      541 tcgcccacgt ttgtgccgtc gcccgattcg agcagacgaa actctttcaa gatccgtgtg
      601 ggcggaatgc cgccgccggt cttctcgaag tgcccgcgca tcggtttggt aacgttcttc
      661 aacctctgcg gcctgtcgtc caccaacccg agctgcacgg cgtcgtaacc gtcaggaccc
      721 gacgcggatt tcttttgcac cacgacacac ggaccagcct tgattaccgt cactggcgta
      781 accgagccgt ccggcgcaaa gatctgcgtc atgccgattt ttcttccgat gatcccgtta
      841 atcatgttgt ctatcctggt taataggtcc tatagactta taagacctat tgcctgccaa
      901 atgctttgat ctcgacgtct acgcctgccg gcaggtcgag cttcatcagc gcatccaccg
      961 tctgcggcgt cgggtcgaga atgtccatca gccgcttgtg tgtgcgtatt tcgaattgct
     1021 cgcgcgactt cttgtcgacg tggggcgagc gcagcacggt ccacttgttc ttcaccgtcg
     1081 gcaacgggat tggaccagca acacgagcac ccgtgcgctt tgcagtctcg acgatttctg
     1141 tcgtcgattg atcgagcacg cggtgatcgt aagccttgag gcgaatgcga atcttttcgt
     1201 tcattaatat ggcctttggt ctttggtctt tggcttttgg tctttgggct ttgggctttg
     1261 ggctttggtc tttgagcttt cctggatcct tcaagaccca agaccaaaga cctaagtcca
     1321 aagacctatg tcccagcttt tattccacaa catccgaaac cgtgccggct cccaccgtgc
     1381 gaccaccttc acgaatagcg aagcgcaagc ccttctccat cgcgatcggc gcgatcaact
     1441 cgatctccat cgccacgttg tcgcccggca tcaccatctc cacaccttcc ggcaagtgcg
     1501 ccactcccgt cacgtccgtg gtgcggaaat agaactgcgg ccgatagcca gtgaagaacg
     1561 gcgtgtgccg ccctccttct tccttcgtca acacatacgc ttccgccttg aacttcgtgt
     1621 gcggcgtaat cgacccgggc ttcgcaatca cctgcccgcg ctcgatgtcc ttgcgctctg
     1681 taccacgcag gagcaaacca acgttgtcgc ccgccacacc ttcgtccagc agcttcttga
     1741 acatctcaac gccggtcaca accgtctttg ttgttgggcg aataccaacg atctctactg
     1801 cctcgttcac cttcaccttg ccacgctcca cgcggcctgt ggccactgtg ccgcgtccct
     1861 gaatcgtgaa gatgtcttcc accggcatca aaaacggctt ctcgatgtcg cgctcgggcg
     1921 tcggaatgta gctgtccact gcgtccatca actccagcac cgtcttctcc cactccggat
     1981 cgccttccag cgccttcaat gccgaacact tgatcaccgg aatgtcgtcg cccggaaact
     2041 catacgcgcc caacaactcg cgcacttcca actccaccaa ctccagcaac tccggatcgt
     2101 cgactgcgtc caccttgttc aggcatacca ccatcgccgg cacaccgacc tgccgcgcca
     2161 gcagaatgtg ctcgcgcgtc tgcggcatcg gcccgtccgt ggcggccacc accagaatcg
     2221 cgccgtccat ctgcgctgcg cccgtgatca tgttcttcac gtagtcggcg tgacccggac
     2281 agtcaacgtg cgcgtagtgc cgattggcgg tctcatactc cacgtgcgcc gtggcgatcg
     2341 tgataccgcg tgctttctct tccggtgcct tgtcgatctg atcgaaggcc acaaactgca
     2401 ctttcggatt attcttcgcc aacaccttcg tgatcgctgc cgtcaacgtc gtcttgccgt
     2461 gatccacgtg cccaatcgtg ccgatgttca cgtgcggctt ggaacggtca aatttttcct
     2521 tcgacatctt ttcctcttct attaataaag gatcgtatta gtcctgaacc tttgctcgct
     2581 ggagcccgcg atgagatttg aactcatgac ctcctcctta ccaaggaggc gctctacccc
     2641 tgagctacgc gggcccgaat cccaaagcgc ttaaaaactg gagcgggcga cgagattcga
     2701 actcgcgacc ctcagcttgg aaggctgatg ctctaccaac tgagctacac ccgcccttaa
     2761 cattgacgat tttcgattgc cgattgtcga tttaacacac gaccgttggc ttcgaaccaa
     2821 tcaaaaatct gcaatcaaaa atccgcaatg gtgcaggggg taggattcga acctacgtag
     2881 ggcgtgagcc cgccgggttt acagcccggt gccattaacc actcgaccac ccctgcatct
     2941 gcttaatatg ccgtggagcc ggcgaaggga cttgaacccc cgaccctctg attacaaatc
     3001 agatgctcta ccaactgagc tacgccggcc cgctatagtg cacaaatagc gcaatccgac
     3061 aaaatgcgga tactaccaag cgacctgcaa aaacgcaagt cgcggtcaga actacagata
     3121 acgaaccaaa atacgcctga ttcgtgctag tattccttca cctcacgcac gtcccaaatt
     3181 gccccttacc tctctactaa tcaaaggaga ctaagacatg gcaacgttcc gcttctcgcg
     3241 agcacttctg cttttgccca ttctgtttct gctagccctc gccggcaccg ccggcgcgca
     3301 aacacccgaa gttgtcgccc caaccgccaa agaaccaatg cacgtcgccg gcaaaaccca
     3361 actttattgc gccggttacg tccggcttca gcgtctgccc aacatgccgc aggtcgtcgg
     3421 cgcggaggaa gaacaggaac aacgaatgtt caccgacggc gacgttgttt atataaatgc
     3481 cggctcgcgc cagggcattg ctgacggcca gaacttccaa ataatccgcc cgcgcggcga
     3541 catcaagggc gtttaccggc aaaagcccgg ttttctcggc acctacatcc aggaagtcgg
     3601 ccagttgcag gtcttgaaag tcggagaaaa cacttcggcc gcacaaatca cttcatcctg
     3661 cggc
//
LOCUS       DASTEY010000207         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_515631, whole genome shotgun sequence.
ACCESSION   DASTEY010000207 DASTEY010000000
VERSION     DASTEY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515631"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="tyrS"
                     /locus_tag="VFO72_03715"
     CDS             <1..1284
                     /gene="tyrS"
                     /locus_tag="VFO72_03715"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9478422.1"
                     /translation="LRSQEIGVRSKEKRRPVQTKTPAIRETTMTIDEQFSYLAKGTVE
                     IIREAELRAKLEKSAATGKPLRVKLGADPTAPDIHLGHTVVIRKLRAFQELGHIAIFL
                     IGDFTGMIGDPSGKSATRPQLTREEIDANAETYKQQIFKLLDPDKTVIDFNSRWMDTL
                     GSAGFLRLSSHVTVRQILERDDFQQRLEANRPLALHEVMYPLVMAYDSVALEADVELG
                     GTDQKFNLLMGRNLQREYGQESQVAMIMPLLEGTDGVQKMSKSLNNYIGINEPPQEIF
                     GKVMSISDDLMWRYYVLCTDLSENDIIALKLSDRNPRDIKADLARKIVADFHSEAAAR
                     EAEEQFNLMFRSKQVPDDVEQRTVASGSWKLPRLLVELELASSMAEARRLIEQGGVYV
                     DGERAPQADLELSAERSSLIQVGKRRFMRVRGDAD"
     gene            1290..2111
                     /locus_tag="VFO72_03720"
     CDS             1290..2111
                     /locus_tag="VFO72_03720"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9478423.1"
                     /translation="MSATVATEEVLVIETPERVPLHFALASIGNRFLACAIDHTLQVL
                     ALTLVLLAGLIIANVSSLENTLQSAPKWVYAVMILLMFLVFSSYFAFFEWLWSGQTPG
                     KRWLKLRVIREDGRPITFWEAAVRNLLRTLDMMPAPFYSIGLISVFVTSRDQRVGDMV
                     GGTVVVREREAEAPLFAEVFASPVSDPALRRSFKPVEFTANLNSLTEAEIQVVETFLR
                     RRWDLSDVPRQWMAWRVALPILYKLRPTYDLATFTYEGFLEELLHRYLREHRFST"
     gene            2346..>2932
                     /locus_tag="VFO72_03725"
     CDS             2346..>2932
                     /locus_tag="VFO72_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478424.1"
                     /translation="MKSEVFILGGKRTPMGEYVGVLKDVSAIDLGAVAARGALEATHV
                     KPEEIDHTIIGNALQTSGDAIYGARHVALKAGVPFDRPALTVNRLCGSGIQSIVSGAH
                     MLQLGEVQSCLVGGMESMSQAPHVIRGARSGFALGQGKLEDSLMVALLDTYCNTPMAG
                     TAENLARKFEISREEQDKYALRSQQEAKRALDAGF"
BASE COUNT          648 a          790 c          870 g          624 t
ORIGIN      
        1 ctgcggtccc aggaaatagg cgtccggtcc aaggaaaaga gacgcccggt ccaaacaaag
       61 acccctgcga tccgggaaac gacgatgacg atagacgaac aattctcata cctcgccaaa
      121 ggaactgtcg agatcattcg cgaagctgaa ctgcgcgcca aactggaaaa gtccgccgcg
      181 actggtaagc cgctgcgcgt gaagctgggc gcggatccga ccgcgcctga cattcatctt
      241 ggccacacgg tagtcatccg caaactgcgc gcgtttcagg agctgggcca tatcgcgatc
      301 ttcctcatcg gcgatttcac cggcatgatt ggcgatccct ccggcaagag cgccacgcgt
      361 ccacaactca cacgcgaaga gatcgacgcc aacgcggaaa cttacaagca acagatcttc
      421 aaactactcg atccggataa gacggtcatc gacttcaact cgcgttggat ggacacgctc
      481 ggctccgccg gctttctgcg cctgtcgtcg cacgtcacag tcagacagat tctcgaacgt
      541 gacgactttc aacaacgact tgaagccaat cgcccgctgg cgcttcatga agtgatgtat
      601 ccactggtga tggcttacga ctcggtcgcg ctcgaggcag acgttgaact cggcgggacg
      661 gaccagaagt tcaacctgct gatgggcaga aatttgcaac gcgagtacgg acaggaatcg
      721 caggtcgcga tgatcatgcc gctgctcgaa ggcactgacg gcgtgcagaa gatgtcgaag
      781 agtttaaaca actacatcgg catcaacgaa ccgccgcagg agatcttcgg caaggtcatg
      841 tcgatctctg atgatttgat gtggcgctac tacgtgctgt gcacggacct ttcggagaac
      901 gacatcattg cactaaaact gagcgatcgt aatccgcgcg acattaaggc tgacctcgcg
      961 aggaagattg ttgcggactt tcactcggag gcagcggcgc gtgaagccga ggaacagttc
     1021 aacctgatgt ttcgcagtaa acaggtgccg gatgacgtcg aacaacgaac agtggcgtcc
     1081 ggatcctgga agttgccgcg gttgttggtg gaacttgaac ttgcgtcttc gatggctgaa
     1141 gcgcggcgtt tgatagagca gggtggtgtt tacgttgatg gcgagagggc gccgcaagcg
     1201 gatttggaac tgagcgcaga acgaagttct ctgattcaag tcgggaagag gcgctttatg
     1261 agagtaagag gggacgcgga ttaacacgga tgtctgcgac agttgcaact gaagaggtcc
     1321 tggtaatcga aacgcccgaa cgcgtgccgt tgcattttgc gctggcgtcg attggtaatc
     1381 ggtttctcgc ctgcgcgatc gatcacacgc ttcaggtgtt agcgctgacg ctcgtcctgc
     1441 tcgccggcct catcatcgcg aacgtttcca gtctggaaaa cacgctacag agtgcgccaa
     1501 agtgggtcta cgcggtgatg atcctgctca tgtttctcgt cttctcgagt tacttcgcgt
     1561 tctttgaatg gctttggagc ggtcagacgc cgggcaagcg ctggttgaag ctgcgcgtga
     1621 ttcgcgaaga tggacggccc ataacgtttt gggaagctgc cgtgcgcaac ctcctccgca
     1681 cactcgacat gatgcccgcg ccgttctatt cgattgggtt gattagcgtc ttcgttacca
     1741 gtcgcgacca gcgcgtcggc gatatggttg gaggaactgt cgtcgtgcgc gagcgcgaag
     1801 cggaagcgcc actgtttgcc gaagtattcg cgagcccggt gagcgatccc gcgttgcgtc
     1861 gttcgttcaa gccggtggag ttcaccgcca acctgaacag tctaacggaa gctgagattc
     1921 aagtggtgga gacatttctt cgccggcgct gggatctatc agatgtgccg cggcagtgga
     1981 tggcctggcg cgtcgctttg ccgatccttt acaaacttcg tcctacttac gacctggcca
     2041 cgttcaccta cgaaggtttt ctcgaggaac tattgcaccg ctaccttcgc gagcatcggt
     2101 tcagcacgta acggtgggtg tcctaagttg tgaacacgct ctttcctggg accgcacgcg
     2161 tctcgcctgc actgagcgcg aagcgcgaaa aacgagcgat gcaggcgaga cgccttcggc
     2221 gttaaattca gactgaccca ctaccgacac tacctgacgg tcctcagaat tgacccggtc
     2281 ctcgtctctt tgttagcctt caaggttttc ttaacagaga gatacgaaac agaggagcac
     2341 gagaagtgaa aagcgaggtt tttatactag gcggcaagcg gacgccaatg ggtgagtatg
     2401 tcggcgtgct gaaggatgtt tcagcgatcg acctgggcgc ggtggccgca cgcggcgcgc
     2461 ttgaagcgac gcacgtcaag cctgaagaga tcgatcacac gatcattggc aatgctttac
     2521 agacttccgg cgacgcgatt tatggcgcgc gtcacgtcgc actcaaagca ggcgttccat
     2581 ttgatcgtcc cgcgttaacc gtcaatcggc tctgcggcag cggtattcag tcgatcgttt
     2641 ctggcgcgca catgctgcaa ctgggcgaag tacagtcgtg ccttgtcggc ggcatggagt
     2701 cgatgtcgca ggcgccacac gtgattcgtg gcgctcgctc ggggtttgcg cttggacagg
     2761 gcaaactcga agacagcttg atggttgcgt tactggacac ttactgcaat acgccaatgg
     2821 ccggcacggc ggaaaatctt gcacggaagt ttgagatctc gcgcgaggaa caagacaagt
     2881 acgcactgcg ttcgcaacag gaagcaaagc gcgcgctgga tgcgggtttt tt
//
LOCUS       DASTEY010000208         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1603076, whole genome shotgun sequence.
ACCESSION   DASTEY010000208 DASTEY010000000
VERSION     DASTEY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603076"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VFO72_03730"
     CDS             complement(<1..882)
                     /locus_tag="VFO72_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478425.1"
                     /translation="MNLIMIIRVAFRALVRNKMRAALTMLGIIIGVSAVIAMVSIGQG
                     ASASVQAQIESIGTNLLFVSAGAQNVGGVRSGTGDTGTNTLTVEDLDAIKREVPSVSM
                     VTPNVNARSQLVAGNMNWNTSVTGVSEQYPEIRKWPVATGSFFADSDVRSAARVIVIG
                     QTLADNLYPGTDPIGQDIRVANLPFRIVGVMARKGQDPQGRDQDDVAFAPYTTVQKKI
                     LGRDRIQIAFVSAISQDATYTAQSQITDLLRQRHKLTANEPDDFTVRNMTDVAEAANE
                     TSKTMTILLACIAGVSLL"
     gene            complement(886..1704)
                     /locus_tag="VFO72_03735"
     CDS             complement(886..1704)
                     /locus_tag="VFO72_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9478426.1"
                     /translation="MSTTFAVEPRPGIVVGAPIPEARPVIELDHIHKIYTMGDVEVHA
                     LRGVSLTIREGEFVAIMGASGSGKSTMMNIVGCLDRPTRGTYILDGEDVSQLSKDERA
                     DIRCQKIGFVFQGFNLLSRTSALENVELPMLYLGVDTATRHQRAMEALASVGLAGREQ
                     NHPNQLSGGQQQRVAVARSLVNNPALILADEPTGNLDSRTSVEVMEIFQRLNRDRGIT
                     LVLVTHESDIAQYAGRVVVFKDGKIKSDQQVQNPRDAAEELKNLPAIDEDDDEE"
     gene            complement(1801..>2132)
                     /locus_tag="VFO72_03740"
     CDS             complement(1801..>2132)
                     /locus_tag="VFO72_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478427.1"
                     /translation="QGQGRRRQQQGGDNSAQGDDGQNRFAPASAPVLPGQVRVVWVMG
                     QDGKPERRRITVGLSDGAATEVVEGDLKEGDMVITGQQISGSNASSNTSRPPGFGNAP
                     RTGGGRR"
BASE COUNT          411 a          659 c          596 g          466 t
ORIGIN      
        1 gagtagtgac acgccggcga tacaggccaa caggatcgtc attgtcttgc tggtctcgtt
       61 cgcagcctcg gcgacgtcag tcatgttccg caccgtaaag tcatccggct cgtttgcggt
      121 gagtttgtga cgctgccgca gcaggtcagt aatctgcgat tgcgcagtgt atgtggcgtc
      181 ctgtgaaatc gcggagacga aagcgatctg aatgcggtct cgtcccagga tcttcttctg
      241 gaccgtggtg tacggcgcaa acgcgacgtc gtcctgatcg cgtccctgtg gatcctgtcc
      301 cttgcgggcc atcacgccga cgattcgaaa cggcagattg gccacgcgaa tatcctgacc
      361 gatcggatcg gtgccgggat acagattgtc agccaacgtt tgtccaatca cgatcacgcg
      421 agcagcggag cgcacgtcag aatcggcaaa gaacgagccg gtcgccacgg gccacttgcg
      481 gatctccgga tactgttcgc ttacacccgt aaccgaggtg ttccagttca tatttccggc
      541 gaccagttgc gatctcgcgt tgacgtttgg cgtcaccatt gaaacgctcg gcacttcgcg
      601 cttgattgcg tcgagatcct cgacagtcag cgtgttggtg ccggtgtctc ccgtaccact
      661 gcgcacgccg ccgacgtttt gcgcgccggc gctgacgaag agcaggttgg tgccgatact
      721 ctcgatctgt gcctgcacag acgccgaggc tccctgtccg atgctcacca tcgcaatcac
      781 ggcggagacg ccgatgatga tgccgagcat agtcagggcc gcgcgcatct tgttacgaac
      841 gagcgctcga aaggcgacgc gaatgatcat tatcaagttc atctgttatt cctcgtcgtc
      901 gtcctcatcg atcgccggca ggttcttcag ctcttcagcc gcatcacgcg gattctgaac
      961 ctgctggtct gacttgatct ttccgtcctt gaagaccacc acgcgtcccg cgtattgcgc
     1021 gatgtcggac tcgtgcgtga cgagcaccag cgtgatgcct cggtcacgat tcagacgttg
     1081 aaagatctcc atcacttcca ctgacgtgcg cgagtcgaga ttacctgtcg gttcgtcggc
     1141 aagtatcaat gccggattat tgacgagtga acgcgcgaca gcgacacgct gctgctgtcc
     1201 acctgacaac tggttcggat gattctgctc gcgccctgcg agaccaactg aggccagcgc
     1261 ttccattgca cgttgatgac gcgtggctgt atcgacgccg agatagagca tcggcagctc
     1321 gacattttca agagctgacg tgcgcgaaag caggttgaaa ccctgaaaca caaagccgat
     1381 cttctggcag cgaatatcag cgcgctcgtc tttcgacaac tgcgaaacgt cttcgccgtc
     1441 aagaatgtaa gtgccgcgtg tcgggcggtc gagacaaccg acaatattca tcatggttga
     1501 cttgccggaa ccggaggcgc ccatgatcgc tacaaactcg ccttcacgaa tcgtgaggga
     1561 aacgccgcgc agcgcgtgaa cttcaacatc gcccatcgtg taaatcttgt ggatgtgatc
     1621 cagttcgatc accggacgag cttccggaat cggcgcaccg acaacgattc ccggccttgg
     1681 ttcgactgca aacgttgttg acatcttctc tcccgaactt tttgtagggg cggccctccg
     1741 tggccgcccc agcgcacgca tcttcaacgc tggggtggcc acggagggcc acccctacta
     1801 ttaccgtcgg ccgccgccgg tgcgtggcgc gttaccgaat cctggcggtc ttgaggtgtt
     1861 cgatgacgca ttcgagccag agatctgttg cccggtgatg accatatcgc cttccttcag
     1921 atcaccttcg acaacttctg tcgccgctcc atcactcaat ccaacggtga ttctgcgacg
     1981 ctcgggtttg ccgtcctggc ccatcaccca gacaacacgc acctggcccg gcagcactgg
     2041 agccgatgcc ggggcgaacc ggttctggcc atcatcaccc tgagccgagt tatcgccgcc
     2101 ctgctgctga cgccggcgtc cctgcccctg ac
//
LOCUS       DASTEY010000209         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_301299, whole genome shotgun sequence.
ACCESSION   DASTEY010000209 DASTEY010000000
VERSION     DASTEY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301299"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /gene="hutH"
                     /locus_tag="VFO72_03745"
     CDS             complement(<1..1271)
                     /gene="hutH"
                     /locus_tag="VFO72_03745"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET9478428.1"
                     /translation="MLQLNGQQLTLQQIADVATGKEHVTLGDEARRRVEQSRSVVEQI
                     VAEKRTVYGVNTGFGKLSDVSIDPADLRQLQLNLVRSHSCGLGEPLSELEARAMLLLR
                     ANVLATGFSGARPLVIETLIAMLERGVTPVIPEKGSVGASGDLAPLAHLALAAIGEGD
                     AFFNGERLAGNVALQRAEIAPLQLEVKEGLALLNGTQAMAAVGALALHRAERLVRLAD
                     VAGAMTLESLRGTPVAFDERIHTARPHAGQVAVAAHLRELLQDSEIRKSHVDNDPRVQ
                     DAYSLRCMPQVHGAVRTALAHASDVVQLESGSATDNPLVFSETGEVLSGGNFHGAPLA
                     LVFDYAAIALTDLISITERRIDRLVNPDANEDLPPFLTAHPGISSGFMMLQVSAVALL
                     NEAKILAHPASVDNVPTDGGKEDHVSMGMTSA"
     gene            1280..1492
                     /locus_tag="VFO72_03750"
     CDS             1280..1492
                     /locus_tag="VFO72_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478429.1"
                     /translation="MPIDGIADFRLPIDGIADFRLPIADFYYWTHTFISSRASGNRQS
                     QIGNRKSKIGNAINRQSKIENRQFLT"
     gene            complement(1485..2432)
                     /locus_tag="VFO72_03755"
     CDS             complement(1485..2432)
                     /locus_tag="VFO72_03755"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HET9478430.1"
                     /translation="MSTEVSQRGSGPLTTERGTGRLRPPPAADPLPETTGQSPSALMR
                     RMSPSEFNTAMIHFYRGEVQRSNTWRNRLDTTTNWAVITAGATLSFVFSSPNNPHFVI
                     PINSILVVIFLLMEARRYRYYEIWSSRVRILETGYFAHLLAPENQAADEAWAEHLAAD
                     LTTPHFTITEWEAIGRRLRRNYLWIFVLLALSWNLKVYLSPLPARDFDAFIERATVGV
                     VPGWIVFVIGVVFNAAIFVFAIGTLRLREATGEVLPKHEFSLRPFKRMTSWTAATTRR
                     TTVRARRARQRVRSAAATGEFKKIDISETPDRPVETGVR"
     gene            2572..>2931
                     /locus_tag="VFO72_03760"
     CDS             2572..>2931
                     /locus_tag="VFO72_03760"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET9478431.1"
                     /translation="MKEAKLVNTAAETEVDEHWMRQALAAAQEARSRGEVPVGTCVVN
                     GGQLIAVSGNRTRTDCDPTAHAEIVALREAAQQLGNYRLTGVDVYSTIEPCAMCAGAL
                     IQARIRRLVYGAADERAG"
BASE COUNT          616 a          861 c          846 g          608 t
ORIGIN      
        1 gccgaggtca ttcccatcga cacatgatct tctttgccgc catcggtggg cacgttatcc
       61 acgctcgccg gatgcgccag aattttcgcc tcgttcaaga gcgccactgc cgagacttgc
      121 aacatcatga accctgacga aatgcctgga tgcgccgtca aaaacggcgg caggtcttcg
      181 tttgcgtcgg gattcaccag acgatcgatg cgccgttcgg tgatactgat caagtctgtc
      241 aaagcgatgg cggcatagtc aaaaacgagc gcgagcgggg cgccgtgaaa gtttcccccg
      301 gacaatactt cgcccgtttc ggaaaacacc agcggattgt ccgttgcact gccactttca
      361 agctggacca catcagacgc atgggccaac gctgttcgta ccgcgccatg cacctgcggc
      421 atgcaacgca aactataggc gtcctgcacg cgcggatcgt tatcgacgtg cgacttgcga
      481 atctcactgt cctgcaacaa ctcgcgaaga tgtgccgcta ccgcaacctg tccggcgtgc
      541 ggtcgtgcag tgtgaatacg ctcgtcaaat gcgaccggcg tgccgcgtaa cgattcgagc
      601 gtcattgctc ccgcaacgtc tgccaaacgc accagcctct cagctcgatg aagcgctagt
      661 gcaccgaccg cagccatcgc ctgcgtcccg tttagcagcg caagaccttc cttgacttcg
      721 agttgcagcg gcgcgatctc cgcgcgttgc aaggcaacat ttccggctag acgttcgccg
      781 ttgaaaaacg cgtcgccttc gccaatcgcc gccagtgcga ggtgggccaa aggcgccaga
      841 tcaccgctcg cgccgaccga tcctttttcc ggtatgaccg gagtcacacc tcgctcgagc
      901 atcgcgatca gcgtctcgat caccagcggg cgcgctccgc taaagccggt ggcgagcacg
      961 ttagcgcgca gcagcaacat cgcgcgtgct tcgagttcgg aaagtggttc acccaaacca
     1021 cacgaatgac ttcggacgag gttcaactgc aactggcgca agtcggccgg atcgatgctg
     1081 acgtctgaaa gttttccgaa gccggtattg acgccgtaga cggtgcgttt ctcggcgacg
     1141 atctgttcga ctacgcttcg tgattgttcg acgcgccggc gcgcctcgtc gccgagtgtg
     1201 acgtgctctt ttcctgtggc tacatctgcg atttgctgga gagtaagttg ttgaccgtta
     1261 agctgaagca tgttgggtat tgccgattga tggaattgcc gattttcgat tgccgattga
     1321 tggaattgcc gattttcgat tgccgattgc cgatttttac tactggacgc acacgttcat
     1381 tagcagtcgg gcaagtggca atcgccaatc acaaatcggc aatcgaaaat cgaaaatcgg
     1441 caatgccatc aatcggcaat cgaaaatcga aaatcggcaa ttccttacct gaccccggtt
     1501 tccacgggtc ggtcgggcgt ttctgatata tcgatcttct taaactcgcc tgtcgccgcg
     1561 gcgctgcgca cacgttgccg cgcgcgtctg gcgcgcaccg tggtgcgccg cgtggtggcg
     1621 gccgtccaac tcgtcatccg cttaaacggc cgcaacgaga actcatgttt cggcaacact
     1681 tcgcccgtcg cttcacgcag tcgcagtgtc ccgatagcaa aaacaaagat cgctgcattg
     1741 aacaccacgc cgattacaaa cacgatccaa ccgggaacga cgcccactgt cgcgcgctcg
     1801 atgaacgcat cgaagtcgcg ggccggcaac ggcgatagat aaacttttaa attccagctc
     1861 aacgcaagca acacgaaaat ccacaaatag ttccgccgca aacgacggcc aatcgcctcc
     1921 cactccgtga tcgtgaaatg cggtgtggtg agatcggcgg cgagatgttc cgcccaggct
     1981 tcatccgcgg cctggttttc cggcgccagc aggtgggcaa agtagcccgt ttcgagaatt
     2041 ctgacgcggc tcgaccagat ctcgtaatag cgatagcggc gcgcttccat caacaggaag
     2101 atgactacga gtatcgagtt gatcggaata acgaagtggg gattgttcgg tgaactaaaa
     2161 acgaatgaga gcgtcgcgcc cgcggtgatt acagcccagt tcgtggtggt gtcgagacgg
     2221 tttcgccagg tgttcgatcg ctgcacctcg ccgcgataga agtgaatcat cgcggtgttg
     2281 aattccgacg ggctcatgcg gcgcatcagt gccgagggtg actggccggt cgtctcggga
     2341 agcgggtcgg cagcaggcgg cggccttagt ctgccggtgc ctctttctgt cgttaatgga
     2401 ccgctacctc tttgacttac ttcggtggac attgggacct gaaccgagtg ggagagcgcc
     2461 gccagaccgg gagcgcggaa ctccggaaag tgataataac acgcgcctac cgccttgtgt
     2521 cgaacgaatt gccaattagc agccggttgg ctatagtggc cgttcggctg tttgaaggaa
     2581 gcgaagttag tgaatactgc cgcggaaact gaggttgatg agcactggat gcggcaggcg
     2641 ctggccgcgg ctcaagaggc acgaagtcgt ggtgaggtgc cggtcggcac ttgtgttgtc
     2701 aacggtggcc aactcatcgc cgtttccgga aatcgcactc gtaccgattg cgatcccacc
     2761 gcccacgccg aaatcgtcgc cctacgcgag gccgcccaac agctcggtaa ctaccggctg
     2821 acgggcgttg atgtttactc gacgatcgaa ccgtgcgcca tgtgcgccgg cgcgttgata
     2881 caagcgcgta tcagacgttt ggtttacggc gcggcagacg aacgcgcggg c
//
LOCUS       DASTEY010000210         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_416774, whole genome shotgun sequence.
ACCESSION   DASTEY010000210 DASTEY010000000
VERSION     DASTEY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416774"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFO72_03765"
     CDS             <1..1154
                     /locus_tag="VFO72_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478432.1"
                     /translation="DGYYDLRITAELWETYYYDQLSLMVVDHPVGTEIFVDERFVIPP
                     AKLAITSVATPRPIASAIDDNGQDVTSAVKALDGNYLDTFGRGRYQGVTRDHFVEIDL
                     GNDAPVSGPFWLIAHGWMHPTDSSINVAISQGGLQAAKPLSLEVPDGRGGWEVARPNL
                     GFPAGRKKTCLFDLSNVFKPGTPRKLRLRTNLEVFWDLLEWAVAVPGAAPKITDLNPE
                     VADLHYRGYSVINQANASSPEIPDYNRLSGSKQIWRDLIGYYTRFGDVRELLLRADDR
                     YVIMNAGDELSFRFAVPPAPPVGWVRDYVIRGDGWIKDGDYNSTFSKTVLPLPYHARN
                     LYDTPPGRLEDEWVYRRFPEDWQNYHTRYVTADTFQNAMRSRLRHGEFK"
     gene            1338..3134
                     /locus_tag="VFO72_03770"
     CDS             1338..3134
                     /locus_tag="VFO72_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HET9478433.1"
                     /translation="MRNSKSARIVLTVIFVSLLAVPLVLKRFRTRGETANVSLGAAAA
                     LSRYGFHLDEVAKSAGVDFVHEVPTLDPKLDHIMPQVASMGAGVSIVDFDRDGWADIY
                     VTNSGEGSDNALYRNLTDGSFKDVAAEMGIADLNQRVTGVSMGAVWGDYDNDGYEDLF
                     LYKWGRPELFHNDAGQRFTRVTEKAKLPAWVNANTAIWFDYDRDGRLDLFLGGYYAED
                     VDLWHLKTTKMMPESFEYARNGGRKYLFHNLGDGLFEEVSEKLGIDTRRWALAAAAAD
                     LRGTGYPDLFIANDYGISELYFNDGKRFREVGKQTGVGFSPKSGMNASMADILNSGKY
                     AIYVSNISEEGVLIQGNNLWVPREGTAGDSLKFDNLARAMDVEMGGWSFGAQFGDLNN
                     DGTLDLYLVNGYISGDRNTNYWYDFSKIAGGNSTIISDAAQWPSMDGRSLSGYQHKKV
                     WLNDGTGKFVNVAQAVGAGDVYDGRAVALVDLWNRGVLDVVVANQKGPLLIYKNTVAP
                     DNKWIGFELEGSASNRSAIGAEVRVFWDGRQQVQQVTGGSGFCSQNQRRLHFGLGKNP
                     RLERVEIRWPSGKTQTLDKAEIGQVYKLKESA"
     gene            3131..>3562
                     /locus_tag="VFO72_03775"
     CDS             3131..>3562
                     /locus_tag="VFO72_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478434.1"
                     /translation="MKIRFDNRYIPPLFITCILLVGHLFYGILESYERTALAIVSAII
                     AELVLGRIFFGKWLNLASGYITGISVGILLRSPAFWPYALCSVVSIMSKYVLRLKDRH
                     LWNPSNFGICALLLLAPDTVAVLSIQWGNFIWPMLVIWILGS"
BASE COUNT          759 a          921 c         1078 g          804 t
ORIGIN      
        1 gcgacggtta ttacgacctc cggatcaccg cagagctttg ggagacttac tactacgatc
       61 aactcagttt gatggtggta gatcacccgg tgggaacaga aatattcgtg gatgagcggt
      121 tcgtcattcc gccggcgaag ctcgccatca caagcgtcgc gacgcctcga ccgatcgctt
      181 cagccatcga tgacaacggt caggacgtca ccagcgctgt caaggcactc gatggaaact
      241 acctcgacac tttcggccgc ggcaggtatc aaggcgtcac gagagatcat ttcgttgaga
      301 tcgatctcgg caacgatgcg cccgttagcg gtccgttttg gctgatcgcg cacggctgga
      361 tgcacccgac ggattcatca atcaacgtgg ccatcagcca gggcgggctc caagcggcga
      421 agcctttgag tttggaagtt cctgatggcc gcggcggctg ggaagtcgcg cggccgaatc
      481 tcgggtttcc tgccggcaga aagaaaactt gcttgttcga cctgtcgaat gttttcaagc
      541 cgggcacgcc ccgcaagctt cggctgcgta ccaatctcga agtcttttgg gacctgctcg
      601 agtgggcggt tgcagttccc ggcgcagcac caaagatcac agatttaaat ccggaggtgg
      661 cagatcttca ctatcgcggt tattctgtca tcaatcaggc gaacgcttcg tctcctgaaa
      721 taccagacta caaccgcctt tcgggatcga aacagatctg gcgagatttg attggttact
      781 atacgcgatt tggcgacgtg cgcgagttgc tgctgcgcgc cgacgatcgt tacgtaatta
      841 tgaatgccgg tgatgaactg tcgttccgct ttgccgtacc gccggcgcca cccgtgggtt
      901 gggtgcgcga ttacgtgatt cgtggcgatg gttggattaa agacggagat tacaattcca
      961 ctttttcaaa gactgtgttg ccgttgcctt atcacgccag gaacctgtac gacacgccgc
     1021 ccggcaggct ggaagacgaa tgggtgtatc gtcgttttcc ggaagactgg cagaactatc
     1081 acacgcgcta cgtgacggct gacacttttc aaaacgcaat gaggtcgcgg ttgaggcatg
     1141 gcgagttcaa ataaataagg cacgtcttcc ctgggaccgc aggcgtctcg cctgctgtga
     1201 tgcgaaggag gccggttgcc aggaatgcga gctgagacgc ctgctgtgat gcggtagggg
     1261 cccgctgcca ggaatgcagg cgagacgcct gcggtcccag gaaagacacg tccgttccgc
     1321 gaagtgcgtc actaaagatg cgtaatagca agtcggcaag aatagtctta acagtgatct
     1381 tcgtttctct actggcagtg ccgctggtat tgaaacgttt tcgcacgcgc ggcgagacgg
     1441 cgaacgtttc actcggcgcc gcggccgcgc tcagccgtta cgggtttcat ctcgacgaag
     1501 tagcaaaatc cgcgggtgtt gattttgtcc atgaggtgcc cacgctcgat cccaagttgg
     1561 atcacatcat gccgcaagtt gcttcgatgg gcgcgggcgt ttcgatcgtg gatttcgatc
     1621 gcgacggctg ggccgacatc tacgtcacca acagcggcga gggaagtgat aacgcgctct
     1681 accgcaacct gacggacggc tcgttcaaag acgtggccgc ggagatggga atcgcggatc
     1741 tgaatcaacg cgttactggc gtttccatgg gcgcggtttg gggcgactac gacaacgacg
     1801 gttacgaaga tctgtttttg tacaagtggg gccgccccga actattccac aacgatgccg
     1861 gacagcggtt cacgcgtgtg accgagaagg cgaaactgcc cgcgtgggtc aacgcaaaca
     1921 ccgccatctg gttcgactac gatcgcgacg ggcgcctgga tctgtttctc ggtggttatt
     1981 acgcggaaga cgtcgatctc tggcatctga agacgacgaa gatgatgccg gagagtttcg
     2041 agtacgcgcg aaacggcggg cggaagtatc tcttccacaa cctcggagac ggattgtttg
     2101 aagaggttag cgaaaagctg gggattgata cgcgaaggtg ggctttggca gcggcggcgg
     2161 cggatttacg cgggacaggt tatccggatc ttttcatcgc caacgactac ggtatctctg
     2221 aactgtactt caacgatggg aagcgctttc gcgaagtcgg taaacagacc ggcgtcggct
     2281 tctcgccaaa gagcggcatg aacgcttcga tggctgacat tcttaacagc ggcaagtatg
     2341 caatctacgt ttcgaacatc tcggaagaag gcgtgctcat tcaaggcaac aatctttggg
     2401 tgccgcgcga aggaactgcg ggcgactcgt tgaagtttga caaccttgcg cgcgctatgg
     2461 acgtggagat gggtggctgg agctttggcg cgcagtttgg cgatctaaac aacgacggca
     2521 cgctcgatct ttatctggtt aacggttaca tctccggcga tcgaaacacg aactactggt
     2581 acgacttctc gaagatcgcg ggcggtaact ccaccattat ctccgacgcc gctcagtggc
     2641 cctcgatgga cggccgcagc ttgtcgggtt atcaacacaa gaaagtttgg ctgaacgacg
     2701 gcaccggaaa gtttgtaaac gtggcccagg cagttggcgc gggtgatgtt tacgacggcc
     2761 gtgcagtcgc gttggtggat ctgtggaatc gtggcgtgtt ggacgttgtg gttgcaaatc
     2821 aaaaagggcc gttactgatc tacaagaaca cggtcgctcc ggacaacaag tggatcggct
     2881 tcgaactcga aggctcggcc agtaaccgca gcgcgatcgg cgcggaagtg cgtgtgtttt
     2941 gggacggccg gcagcaggtg caacaggtga cgggtggcag tggtttttgt tcgcagaacc
     3001 agcggcgatt gcacttcggc ttggggaaaa atccccgcct tgaaagagtc gaaatccgct
     3061 ggccgtcagg aaaaactcaa accttggaca aagctgaaat cggccaggtg tacaagctca
     3121 aagagtctgc atgaagattc gttttgataa ccgttacatc ccgccgctct tcatcacctg
     3181 cattctgctg gtagggcatc tcttctacgg gatcctggaa agctatgaaa gaactgcgct
     3241 cgcgattgtt tctgcaatca ttgcggagct ggttctggga cggatctttt ttggcaagtg
     3301 gctaaacctg gcgagtggct acatcacggg catcagcgtt gggatcttgc ttcgttcgcc
     3361 tgcgttctgg ccgtacgcgt tgtgcagtgt ggtctcgatc atgtcgaagt acgtgttgcg
     3421 cctgaaggac cgtcaccttt ggaacccttc caacttcggc atctgcgcgt tgttactgct
     3481 ggcacctgac acggttgcgg ttctgagtat ccagtggggc aatttcatct ggccgatgct
     3541 ggtgatttgg atcctcggtt cg
//
LOCUS       DASTEY010000211         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_680341, whole genome shotgun sequence.
ACCESSION   DASTEY010000211 DASTEY010000000
VERSION     DASTEY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680341"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VFO72_03780"
     CDS             <1..890
                     /locus_tag="VFO72_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478435.1"
                     /translation="MFPVTVRDASGRLINDLTRTDFRVYEDNRLQPLSDLALRQVPVD
                     VVLMVDASSSVANNLDDFRRAAEGFAARLQTDDRISLIKFDDRVELLQDWTKSRFQLK
                     RALNRIEPGMFTRFNDALLLASREQFGATTKSRRAVIVLSDGIDSGRGMATLETALQG
                     LIQAQVTVYVVSNTEISRANKRAELDQLLSSTGGAAQFNQLRIEDLREGLRVLDRSEQ
                     NLAQLAQATGGRLYKPKTFDALESTYAEVADELRHQYALYFTPLNKARDGSFRRVRVE
                     TVNPGFKPHTRVGYFAPKT"
     gene            904..1461
                     /locus_tag="VFO72_03785"
     CDS             904..1461
                     /locus_tag="VFO72_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478436.1"
                     /translation="MWKKLAPCLLVIFCAVPCAQAQTDKEGITIVLVGNRTVVVDEVS
                     ESSDGYWYKRGNVTTFLDRERVVRVEYPKPPEAPAGSEPLEGTGKWRLTDAQKIQDFF
                     LAKFKKRLPTTAFGQSDLHTRWGLDHRNGMDVGLHPDSTEGRALIEFLRDASIPFLAF
                     RGPVPGVATGPHIHIGNRSARVSGR"
     gene            1503..>3482
                     /locus_tag="VFO72_03790"
     CDS             1503..>3482
                     /locus_tag="VFO72_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET9478437.1"
                     /translation="MKFIDFAHPEGGRLLVHVDHITSAHYREGEGDVKSRLGLDLDER
                     QNDVVLFGEEADRTWEKLRRLMGESGALNRILLCASVSRWLISFHVKPQSHRAHRENL
                     KAGHYVGEIALILTDSLSEQWIDLRLIAGMRVVLGTSALLVSILGAGEFRPRAVPTDV
                     ALVLYSLYGLLIYQLSVRRSRVAAHKLIPWLDLLWYLPLIWLSGTNGTFYYFFFFAIV
                     VASFSSGLTDGLRLTLASAVSYTLIGLTARHGRPLQMNQLLMAPIGLLTFGYIIARWG
                     GYHTDLKNRLKLLKDVTVFSNPRYGIDRTIKAIMESIRAFYDAEACLLVIPGKPGAES
                     YQLYRVTRDTTPSRSAPPEIGRDAAGQFLAPSLDNAVIYRRNRSSHTRLFDVRSKLFS
                     ESGSTESDKLAVMLDAQRYVSMPVYYRHQPVGRMYIIDGPTRLDTPDMEFLLQLMDHV
                     TPVMENIRLIDNLASDAAEQERRRIAHDIHDSVIQPYLGLQFGLAALDQKLETGNGSV
                     RGDIQELLELTNHELAELRRFVWGLRAGEERTDVLLPAIERYAERFSLVTGIKVDVEA
                     RGKVKVNDRLAAELFQIVAEGLSNVRRHAFCRDARVEISCKNSSLLLQIKNSRPRTNP
                     ESERGDGTENVRSFRPHSIAERAASLGGDTSVFIDE"
BASE COUNT          760 a         1023 c          950 g          749 t
ORIGIN      
        1 tcatgttccc ggtaacggtg cgcgacgctt cgggacggct gatcaacgat ctcactcgca
       61 ctgactttcg cgtttatgaa gacaacagac tacaaccact gagcgatctc gcgttgcggc
      121 aggtgcccgt tgacgtagtg ctgatggttg acgcgtcctc gtcagtagcc aacaatctcg
      181 acgactttcg tcgtgcagcc gaaggattcg ccgcacgtct gcaaactgac gatcgcatca
      241 gcttgatcaa gtttgacgat cgcgtcgagc tacttcagga ctggaccaag agccgtttcc
      301 aactgaagcg cgccttgaat cgtatcgagc ccggtatgtt cacgcgcttc aacgacgcgc
      361 tgctgctggc atcgcgcgag cagtttggag caacaacgaa atcgcgccgt gccgtcattg
      421 tgttgtcgga cggaatcgac agcggccgcg gcatggccac gctggagacg gcgttgcaag
      481 gactgattca ggcgcaggtg actgtctacg tcgtcagcaa caccgaaatc tctcgcgcta
      541 acaaacgcgc cgagctcgac caactactta gctcgacagg cggcgcagcg cagttcaatc
      601 aactccgcat cgaagacctg cgcgaaggcc tgcgcgttct cgatcgcagt gaacaaaacc
      661 tggcccaact cgcccaggcg accggcgggc ggctttacaa accaaaaact ttcgatgcgc
      721 tcgaatcgac ttacgcggaa gtcgcagacg aactgagaca tcagtacgcg ctctatttca
      781 cgccactgaa caaagcacgt gacggaagtt tccgccgcgt acgcgtcgaa acggtgaacc
      841 cgggtttcaa gccgcacacc cgcgtaggat acttcgcgcc aaagacatag gtcggtgttg
      901 tccgtgtgga aaaaacttgc cccttgctta ctcgtcatct tttgcgcagt tccctgcgct
      961 caagcccaga ctgacaaaga ggggatcacg attgtcctgg taggaaatcg aaccgtcgta
     1021 gttgacgaag tttcagagtc aagcgacggc tactggtaca agcgcggaaa cgtcactacg
     1081 tttctcgatc gagagcgcgt cgtgcgcgtt gagtatccca agccacctga agcgcccgcc
     1141 gggtctgaac cgttggaagg aaccggcaag tggcgactca ccgacgctca gaaaattcag
     1201 gatttctttc tcgctaagtt caaaaagcgt ttgccgacga cagccttcgg acagtcagat
     1261 ctccacacgc gctggggatt ggaccatcgc aatggcatgg acgtcgggct acatcccgac
     1321 agcaccgaag gccgcgcgct aatcgagttt ctgcgcgacg catcgattcc ctttctggcc
     1381 tttcgcggcc ctgttcccgg cgtggccacc ggccctcaca tccacattgg aaacagatct
     1441 gcgagagttt ccggccgtta gcagattcga tcgtttccgc aacactctgt tgaggtatac
     1501 cgatgaaatt tattgacttc gcccatcctg agggcggcag acttctggtc catgtcgacc
     1561 acattaccag cgctcactac cgtgagggtg aaggcgacgt caagtctcgg ctcggtctcg
     1621 atctcgacga acgacagaac gacgtcgttc tctttggcga agaagctgat cgaacgtggg
     1681 agaaactacg aagactgatg ggagaaagtg gcgccctaaa cagaatcctt ctctgtgcct
     1741 ccgtgtctcg gtggttgatt tcgttccatg taaaaccaca gagccacaga gcgcaccgag
     1801 aaaacctcaa agcagggcac tacgtcgggg aaatagcttt gattcttacg gattcacttt
     1861 ctgaacagtg gatcgacctg cgactgatcg cgggcatgcg cgtggtgctc gggacttcag
     1921 ctctcctcgt ctctattctc ggcgccggtg aatttcgtcc ccgggccgta ccgacggacg
     1981 tcgcgctggt tctttactcg ttgtatggtc tgctcatcta tcagttgtcc gtgcgccgca
     2041 gtcgtgtcgc cgcacacaag ctgattccat ggctcgatct cctctggtac ctgccgctca
     2101 tttggcttag tggcacgaac ggcaccttct actacttctt tttctttgcg atcgtagtcg
     2161 catctttcag ttcgggcctc acggacgggc tgcggttaac gttagcatcg gccgtgtcct
     2221 acactctcat cggcttaacc gcgaggcacg ggcggccgct ccaaatgaac cagctgctga
     2281 tggcgcccat cggcctgctc acattcggct acatcatcgc gcgctggggc ggttatcaca
     2341 ctgatctcaa gaaccggttg aagctcttga aagacgtcac cgtcttttca aatccccgtt
     2401 acggcatcga tcggaccatc aaagccataa tggaaagcat tcgcgctttc tacgatgcgg
     2461 aggcctgcct gctggtaatc cccggcaaac ccggtgcgga aagttaccag ctatatcgcg
     2521 tgacgcgcga caccacaccg agccgttctg cgccgcccga gatcgggcgc gacgcggccg
     2581 ggcagttcct cgcaccttcg ctggacaacg cagtgatcta ccggcgcaat cgttcgtcgc
     2641 acacgcggct ctttgacgtt agatcgaaac tgttttccga gtcaggatcg actgaatccg
     2701 acaagctcgc cgtcatgctc gacgcacagc gctacgtttc catgccggtc tactatcgcc
     2761 atcaaccggt cggacgcatg tacatcatcg atgggccaac acgactcgat acgcccgata
     2821 tggagtttct gttgcagttg atggaccacg taacaccggt catggaaaac atccgattga
     2881 tcgacaacct tgcttctgac gcagcggaac aggaacgtcg ccgcatcgcg cacgacattc
     2941 acgacagcgt tatccaaccg tatctcggtt tacagtttgg tctcgccgcg ctcgaccaaa
     3001 aactcgagac aggaaatggt tccgtgcgcg gcgacatcca ggaactgctc gagttaacta
     3061 atcacgaact cgccgagttg cgccgttttg tttggggctt gcgcgccggc gaagaacgca
     3121 ctgacgtgtt gctgcccgca atcgaacgat atgcggaacg attctcgctc gtgaccggaa
     3181 tcaaggtcga cgtcgaggcg cgcggcaaag taaaagtgaa cgatcgtctg gcagcggaac
     3241 tgtttcagat tgtggcggaa ggcttgagca acgtgaggcg ccatgctttt tgtcgggacg
     3301 cgcgagttga gatatcgtgt aaaaacagtt ctctgctgtt gcagatcaag aacagccggc
     3361 cgcgaactaa cccggagtcg gaacgcggcg acggcactga aaatgtcaga tcgttccggc
     3421 ctcattcgat cgccgaacgc gccgcttcgc tgggtggcga tacaagtgtt ttcatagacg
     3481 aa
//
LOCUS       DASTEY010000212         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1487981, whole genome shotgun sequence.
ACCESSION   DASTEY010000212 DASTEY010000000
VERSION     DASTEY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487981"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFO72_03795"
     CDS             complement(<1..339)
                     /locus_tag="VFO72_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9478438.1"
                     /translation="MHASRHDQKLEFILRTAARIFAEKSYHSTSMRDISRETNVSLAG
                     LYHYCRSKEELLFMIQDNCFGRVLERLEKQLEEVKDPVARLRVFIENHLSFFAANMAE
                     MKVLSHEAESL"
     gene            complement(344..1747)
                     /gene="boxB"
                     /locus_tag="VFO72_03800"
     CDS             complement(344..1747)
                     /gene="boxB"
                     /locus_tag="VFO72_03800"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HET9478439.1"
                     /translation="MTMNLYDRIPNNVNLAEDKKLQRALEKWLPNYLDWWREMGPDGF
                     QDKDVWLRTAVDVGSEGWAHFDYVKMPDYRWGIFLEPPKPDAIVGFGDNYGRPAYQEV
                     PGEFRNALRRIIVTQGDTEPASVEQQRQLGKTCPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLHGYFGRDGRDEADALLERRSGNPDKPRILEAFNQPCDDWLNFFAFTMFTDRDGKYQ
                     LSALAESGFEPLARTCQFMLTEEAHHMFVGDTGLGRVVRRTAHLMKEGDVRELGGIPL
                     DIVQRYINFWFSYSLDLFGGEISSNSADFFAAGLKGRYQEQKQYTEHTAVNEVFTMQV
                     VEDNQLKPREVPMRNALNEVLRGEYVKDCEKGLARWNKILAEEGLTERLYLPNPHFHR
                     HVGEYAGHTFDPHGNLISREDFEKRKAEWLPTMEDREYVRSLMHPVTEIGKIANWIAP
                     PPAGIKGKPFEFEYVRL"
     gene            complement(1860..>2182)
                     /locus_tag="VFO72_03805"
     CDS             complement(1860..>2182)
                     /locus_tag="VFO72_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478440.1"
                     /translation="IFGDKKVLDTKQAEELGLITFAPDDLDWEDEIRVAIEERTSLSP
                     DALTGMEASLRFAGPETMDTKIYGRLTAWQTWIFQRPNAVGEKGALTNYGKPTQAHFD
                     FKRT"
BASE COUNT          465 a          618 c          572 g          527 t
ORIGIN      
        1 cagtgactca gcttcgtgcg agagcacttt catctccgcc atattcgcgg caaagaacga
       61 aagatggttc tcgatgaaga cgcggagcct ggcgacggga tctttaactt cttccaactg
      121 cttttcaagc cgttcgagca cgcggccaaa acagttgtcc tgaatcataa acagtaactc
      181 ctctttggag cggcagtaat gatagagacc cgcgagactg acgttagttt cacgcgagat
      241 gtcgcgcatc gaagtcgagt gataactctt ctccgcgaag atacgcgcgg cagtgcgcag
      301 aataaactcg agtttttgat cgtgacgaga agcgtgcaat acttcaaagc ctcacatatt
      361 caaactcaaa cggcttgcct ttaatgccgg ccggcggcgg cgcaatccag ttcgcaatct
      421 tgccgatctc agtcaccgga tgcatcaggc tgcgcacgta ttcgcggtct tccattgtcg
      481 gcagccactc cgctttacgt ttctcaaagt cctcgcgcga gatcagatta ccgtggggat
      541 cgaatgtgtg acccgcatac tcgccgacgt gacggtgaaa atgcggattt gggagataaa
      601 gccgttcggt taatccctct tccgcaagga tcttattcca gcgtgcgaga cctttctcgc
      661 aatccttcac gtactcgccg cgcagcactt cattcaacgc atttcgcatc ggcacttcgc
      721 gcggtttcag ttggttgtct tcgaccacct gcatcgtaaa cacttcattc actgcagtgt
      781 gttcggtgta ctgcttctgc tcctgatatc gtcctttcaa accggcggca aagaagtcgg
      841 cggagttgga actgatctca ccgccaaata gatcgagcga ataactgaac cagaagttga
      901 tgtacctctg aacgatatcc agcggaatgc cgccgagctc gcgcacgtcg ccttctttca
      961 tcaaatgcgc cgtacgccgg accacgcggc caagacccgt gtcgccgaca aacatgtggt
     1021 gcgcttcttc ggtgagcatg aactggcacg tgcgcgccag tggttcaaaa ccggattcgg
     1081 ccagcgcgga aagctgatac ttgccgtcgc gatcggtaaa catcgtgaac gcgaagaagt
     1141 tcaaccagtc gtcacacggc tgattgaatg cttcgagaat gcgtggctta tcaggatttc
     1201 cgctacgccg ttcgagcaac gcgtcggctt cgtcgcgacc gtcgcgtccg aagtagccgt
     1261 gcagcaggta gaccatggcc caaagatggc ggccttcttc tacgttcacc tggaagagat
     1321 tgcgcagatc gtagagcgag ggacacgtct tgccgagttg tcgttgttgt tcgactgacg
     1381 ccggttcggt atcgccttgc gtgacgatga tgcgccgcag cgcgttacga aactcgccgg
     1441 gcacttcctg gtaagcgggc cgcccgtagt tgtcgccaaa accgacaatc gcgtccggct
     1501 tgggtggttc gagaaagatg ccccaacggt aatcaggcat cttcacgtaa tcgaagtgcg
     1561 cccatccttc ggagccgacg tcaactgcgg tacgcagcca gacgtctttg tcctgaaatc
     1621 cgtccggacc catttcgcgc caccagtcga gatagtttgg cagccatttc tcgagcgctc
     1681 gctgaagctt tttgtcttcc gccagattta cgttatttgg aatgcgatcg taaagattca
     1741 tagtcattgc cgattttcga ttgtcgattg ccgattgccg ctgccgcgac tgtcaaatgc
     1801 cgcttgcccg ctggccaact gcttcaatcg gcaattggca ataggcaatc ggcaatgtct
     1861 tacgttcttt tgaagtcgaa gtgtgcctgc gttggctttc cgtaattagt caacgcaccc
     1921 ttttcaccta ccgcattcgg acgctgaaag atccaggtct gccacgccgt cagacggccg
     1981 tagatttttg tgtccatcgt ttctggtccg gcgaaacgta atgaagcttc catgccggta
     2041 agcgcatccg gcgagagcga cgtgcgctcc tcgatggcga cgcgaatctc gtcttcccag
     2101 tcgagatcgt ccggcgcgaa agtgatcagt ccgagttctt ctgcctgttt cgtatctaac
     2161 actttcttgt cgccgaagat ct
//
LOCUS       DASTEY010000213         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1372588, whole genome shotgun sequence.
ACCESSION   DASTEY010000213 DASTEY010000000
VERSION     DASTEY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372588"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /gene="rpmE"
                     /locus_tag="VFO72_03810"
     CDS             <1..155
                     /gene="rpmE"
                     /locus_tag="VFO72_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET9478441.1"
                     /translation="KGDLHVEICSACHPFFTGKQKLVDTAGRVDRFNKRYGKKPAAEG
                     AATTAK"
     gene            348..1559
                     /locus_tag="VFO72_03815"
     CDS             348..1559
                     /locus_tag="VFO72_03815"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9478442.1"
                     /translation="MSTPERDLIVGGQAVIEGVMMRTPNAYAIAVRKADGTIVNTAAR
                     LPKWSDKYPLLKLPVLRGGAVLVQSMGLGIKALNYSANEAFEQIEEAKTKEVKVALSP
                     AVIEGEGDFAGLTGAVPGLIPVPTQKRSRDELKKGTTAAAAGSIIFALIFNILLFVAA
                     PLLLTNALFIAAGWATTPPPVTSTASPGASAEGSSSAAQPATETGEPSSIPWFYSAWS
                     SVRTYLHPVRPSVAFNLIDGLIRMAFFLIMIVSFSLLKDIRRVFEYHGAEHKTVFTWE
                     AGLPLTVENARPQPRQHPRCGTSFLMVVMLVAIILFSLIKFDSLLLNFLIRLALMPVV
                     AGISYEIIRLSAKKESSWFFKLMTRPGVWLQNITTQEPDDKQLEVAIEALKESLKLEP
                     QTGEPVPAPLS"
     gene            1591..2667
                     /gene="prfA"
                     /locus_tag="VFO72_03820"
     CDS             1591..2667
                     /gene="prfA"
                     /locus_tag="VFO72_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9478443.1"
                     /translation="MLDKLEQIVTRYDELTAELSSPELLANPSAYAKAAKQHRSLEEV
                     VQKYQAWKGLKAELAGARELYDTSDDDEMREMARLELDTLQARLEENETQLKLLLIPS
                     DPNDEKNVILEIRAGTGGDEATLFAAEMVRMYSRYAEKQRWKFEVLEASESGIGGLKE
                     AIVLIEGVKVYSKLKHESGVHRVQRVPQTEASGRIHTSAVTVAVLPEAEEVDVKIDPK
                     DLRIDTFCSSGPGGQSVNTTYSAVRLTHLPTGVVVSMQDEKSQIKNREKAMRVLRARL
                     QELEEQKQHDAQASERRSMVGSGDRSEKIRTYNFKENRVTDHRIGLTIHQLDLVMEGN
                     LDPFIDALVAHYQAEKLKAEAVAA"
     gene            2831..3463
                     /locus_tag="VFO72_03825"
     CDS             2831..3463
                     /locus_tag="VFO72_03825"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9478444.1"
                     /translation="MPLSTHVLLIRHGQSEGNAERRFGGHTATPLSPRGRRQANATAV
                     ALKNEGLTTIYSSDLARAVETAQPLAGLTGLPVNTSVAFRERSVGVMEGLTFEDAAQQ
                     HPEQYAALLRRDFEHVLTGGESYRQLLDRAWQKLDEIIAENKGGRIAVFSHTGTICIL
                     ALHLMGALDAPELKPVWITSANCGVTRFELREDGFVRVLTLNDTRHLSGT"
     gene            complement(3445..>3816)
                     /locus_tag="VFO72_03830"
     CDS             complement(3445..>3816)
                     /locus_tag="VFO72_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478445.1"
                     /translation="GPGTPYFEDAPALFSTALAQIDFAHDPAAAQDALTWVLNQARPR
                     DTLTLWHLLSRVNEAERVRVYEALTKFVPPPAGVTREGVLQLNKEMLERWKVELEASW
                     GWRGKLDLKKLLKKNAYVPER"
BASE COUNT          864 a         1069 c         1107 g          776 t
ORIGIN      
        1 aaaagggcga cctgcacgtg gaaatctgtt ccgcctgcca tccgttcttt accggaaagc
       61 agaagctggt agataccgcc ggacgcgtgg atcgcttcaa caagcgttac ggcaagaaac
      121 cggcagccga aggcgctgcc accaccgcga agtaacccct gaattgatgg gtttacgcaa
      181 ccaacattag caaccacatc tcggagccgg ctgatcgggt cggtcgctcg aagtgcatca
      241 gagggtgcat caaagaccag cgactgttcc caacaatcgc cggccttatt atgtaggaca
      301 gacattcttg tctgtcccga caggctgaaa agcctgtcct actaattatg agtacaccag
      361 aacgagatct cattgtcggc ggccaggcag ttatcgaagg cgtcatgatg cgcacgccaa
      421 acgcctatgc catcgccgtg cgcaaagcgg acggcacgat cgtgaacact gccgcgcgcc
      481 tgccgaaatg gagcgacaag tatccattgc tgaagctgcc ggtgttacgc ggcggcgcgg
      541 tgctggttca gtcgatgggt ctcggaatca aggcactcaa ctactcggcc aacgaagcgt
      601 tcgagcagat cgaggaagcg aaaacgaaag aggtgaaggt tgcgctttcg ccggccgtga
      661 tcgagggcga gggcgacttt gcgggtttga ctggcgcggt gcccgggttg attcccgttc
      721 ccacacagaa acgttcgcgc gatgagctga agaaaggcac aacggcggcg gccgcaggat
      781 cgatcatctt tgcgttgatc ttcaacatcc tgctctttgt cgctgcgccg ctgctattga
      841 cgaacgcatt gttcattgcc gccggttggg ccacaactcc gccgccggta acaagcaccg
      901 cgtcgccggg tgcttccgcc gaaggttcca gtagcgccgc acagcctgcg actgaaaccg
      961 gcgaaccgtc gtcaataccc tggttttata gcgcctggtc cagcgtgcga acctatttac
     1021 acccggtgag gccgtcagtc gcgttcaacc tgatcgacgg cctgattcgc atggccttct
     1081 tcctgatcat gatcgtcagc ttctcgctgc tgaaggacat caggcgcgtc tttgaatatc
     1141 acggtgcgga acacaagacc gtgttcacgt gggaagccgg actgccgctg acggtcgaga
     1201 acgcgcggcc gcaaccacgc cagcatccgc gttgcggaac gagctttttg atggtcgtga
     1261 tgctcgtagc aatcattctc ttctcgctga tcaagtttga ctctctgctg ctgaactttt
     1321 tgattcgtct cgcgctgatg cctgttgttg ccggcatctc ttacgaaatc attcgcctct
     1381 cagctaagaa ggagagtagt tggttcttca aactgatgac gcggccgggc gtctggctgc
     1441 aaaacatcac gacgcaggaa ccggacgata agcaactcga agtagcgatc gaagcgttga
     1501 aagagtcgct caaactggag ccgcaaacag gcgagcccgt gccggcgccg ttatcgtgag
     1561 gttttcaacg gcgttttttg tgaggtggtc atgttagaca agctcgaaca aattgttacc
     1621 cgctacgacg agttgaccgc ggagttgtcg tctccggagt tgctggcgaa tccgtcggcg
     1681 tacgccaaag ccgctaagca acaccgctcg cttgaagagg tggtccaaaa gtatcaggcg
     1741 tggaaaggac tgaaggcgga gttagccggc gcgcgcgaac tgtacgatac ctctgacgac
     1801 gacgagatgc gcgaaatggc gcgactcgaa ctcgatacac tgcaagcgcg tctcgaagag
     1861 aatgaaacac aactcaaact gcttttgatt ccgagcgatc cgaatgacga gaagaacgtt
     1921 attctcgaaa tccgcgcggg caccggcggc gatgaagcga cattgtttgc cgcggagatg
     1981 gtgcgcatgt actcgcgtta cgccgagaag cagcgctgga aattcgaggt tttggaagcg
     2041 tcggagtcag gaatcggcgg tctcaaagaa gcgatcgtgt taatcgaagg agtcaaggtt
     2101 tactcgaaac tgaaacacga gtcgggcgtg catcgtgtac agcgcgtgcc gcagacggag
     2161 gcgagtggga gaattcatac ttcggcagtc acggtggccg tgttgcctga agcggaagag
     2221 gtcgacgtga agatcgatcc gaaagacttg cgcatcgata cgttctgttc gtcaggtccc
     2281 ggcgggcagt cggtgaacac gacatactcc gctgtgcgac tgacgcacct gcccacgggc
     2341 gtcgttgttt caatgcagga cgagaagtcg cagattaaaa accgtgaaaa ggcgatgcgg
     2401 gtgttgcgcg cgcgtttgca ggagctcgaa gagcagaagc aacacgacgc gcaggcgagt
     2461 gagcgccgtt cgatggtcgg ctcgggcgac cgctccgaaa aaattcgcac ttataacttc
     2521 aaggaaaacc gcgtcaccga tcaccgtatt ggcttgacga ttcaccagct tgacctggtc
     2581 atggaaggaa acctcgatcc tttcatcgac gcgctggtcg cgcactacca ggcggagaag
     2641 ttgaaagcgg aggcggtggc cgcctgaatt gcgggttacg acggtgcttt gctccgcagg
     2701 agcagcatgt ttatagcctg gtgttaaaaa cacatcgcgc tccgtaggag cgccatgtct
     2761 cgctagtccg aagctataaa cattgtgctc cttcggagca gagcctcaac taaagaacca
     2821 caacagttcg atgcccctct caactcacgt ccttctcatt cgccacggcc aatcggaagg
     2881 aaacgccgaa cgacgcttcg gtggccacac ggccacgcct ctgtcacctc gcggacgccg
     2941 gcaggccaac gcaaccgctg tagctctgaa aaacgaagga ctgacgacaa tctactcgag
     3001 cgatctcgcg cgtgccgtcg aaaccgcgca accactggct ggtctgactg gtctaccggt
     3061 taataccagc gtggcgtttc gtgaacgcag tgtcggcgtg atggaaggtt tgacgtttga
     3121 agacgcggca caacagcatc cggagcagta cgccgcactg cttcgtcgcg acttcgaaca
     3181 cgtgttgacc ggcggcgaga gttatcgcca actactcgat cgcgcgtggc agaaactcga
     3241 cgagatcatc gccgagaaca aaggcggccg aattgccgtg ttcagtcata cgggaacgat
     3301 ctgcattttg gcgttgcatc tcatgggcgc gctggacgcg ccggagctga agcccgtctg
     3361 gatcacaagc gcgaactgcg gcgtgaccag atttgaattg cgcgaagatg gtttcgtccg
     3421 cgtgttgact ctcaacgaca cgcgtcacct ttcgggaacg taggcgtttt tctttagcaa
     3481 cttcttcaga tcgagcttgc cgcgccaacc ccagcttgct tcaagctcaa ctttccagcg
     3541 ttcaagcatc tctttgttta gttgcagcac gccttcacgc gtcacaccag ccggcggtgg
     3601 aacgaacttc gtcaatgctt cataaacgcg cacgcgttct gcctcgttaa cccgcgacag
     3661 taaatgccac agcgtcaatg tgtcgcgagg ccgtgcctgg ttgagcaccc aagtcaacgc
     3721 atcttgcgca gccgccggat catgtgcgaa gtcgatttgc gcaagtgccg tgctgaacaa
     3781 cgccggcgca tcctcaaaat aaggcgtgcc tggacc
//
LOCUS       DASTEY010000214         7099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_136625, whole genome shotgun sequence.
ACCESSION   DASTEY010000214 DASTEY010000000
VERSION     DASTEY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136625"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1326
                     /locus_tag="VFO72_03835"
     CDS             367..1326
                     /locus_tag="VFO72_03835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9478446.1"
                     /translation="MEPKANILVVDDNPDKLSLLEAALCLAGYRVTTATDGDEALSAI
                     ESYQPDLVITDVMMPRMNGYELAQRIRANPATRFIPVIMQTAASRRAEDLRRASEVGA
                     LGYITDPTDLDLLLARTRTLLEFKAYLDVCEEAAFTDHLTGLANRRRFERQLEREVGR
                     TMRFGHPFSLLMVDIDNFKNLNDSFGHDAGDDAIRRISKVLREGTRGIDLAARIGGEE
                     FAVLLVETDQRGAIDVAERLRIAIKSLELTSATQITASFGVAECPIDAQTATDILKAA
                     DVALYEAKRAGRDRVVGTPGMRSNSVAAGDVQGDGNLGVASRG"
     gene            1329..2288
                     /locus_tag="VFO72_03840"
     CDS             1329..2288
                     /locus_tag="VFO72_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478447.1"
                     /translation="MADYKEKLGEWQQAARRKARELDEKFAISDLVGDSARVAGDAAR
                     RGAETMASGAERLRAEAERIADDEEMSETARRAASGVKKAGKVVRDVAGDAGKKAGEV
                     FDDARTYYDRASQFYDTGARLTRASTAATAGVLKARDWVKENPGKAAVVSVSLVLGIR
                     AGAAFPGLDAVLLGAQPHWLTHSALPVYGLRKASEKFDSYLKKREELIARGEVDEAER
                     ERIEFERKIAKYVGAPLLGAFSCAAGAAMWAQIFQPGPLVGAPISWLLGGNPFLDGVW
                     LFANGVICFHQGYKFFMIALANQEEVARVVREIKGLLPAAAAS"
     gene            complement(2738..3004)
                     /locus_tag="VFO72_03845"
     CDS             complement(2738..3004)
                     /locus_tag="VFO72_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478448.1"
                     /translation="MRNGDFFDALEGLRAGCDVMDRVEDLGAVISTAAGIADSDYDSF
                     KDNKSVLVLERFPSNLFGPNGPLAVFALVTVRTLLVRFADFIKQ"
     gene            complement(3083..4093)
                     /locus_tag="VFO72_03850"
     CDS             complement(3083..4093)
                     /locus_tag="VFO72_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9478449.1"
                     /translation="MAESFRAVPQGFDLPRAGGLSISAREWRVHGVLFLATIITTTLA
                     GMLLVVPELPPYDPPFSGPFGYLLYVPLAFLHTIVELIRYGLAEPRLIAQGVAFSASL
                     LAILFSHEMGHYLACRYYKVDATLPYFIPAPPLFLAGTFGAFIKIRSPIPSRRALFDI
                     GLAGPLAGFVVALPLSVVGVLTVGPSVAPPEHAIFFNDPLLFRLIAKLFGVPLDPNAL
                     TTPYYMAAWIGLLVTSLNLMPVGQLDGGHGTFAVFGPRAHYVIARVAFVASAVLALLG
                     FWWHNSPSGFLYTILLAVMLRVPHPAPAVMEPLGAKRIAIAILTLIVFILCFWPFPIT
                     IT"
     gene            complement(4187..4501)
                     /locus_tag="VFO72_03855"
     CDS             complement(4187..4501)
                     /locus_tag="VFO72_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9478450.1"
                     /translation="MIKLDIINRVADKTGVPKMKAEQAVEALFHSMKEALTRGERIEL
                     RGFGVFVVKPRKRGVGRNPRTGEEVAIPSGKTIRFKPGKELQAHDNGETEAGESGDDD
                     EF"
     gene            4707..5450
                     /gene="lepB"
                     /locus_tag="VFO72_03860"
     CDS             4707..5450
                     /gene="lepB"
                     /locus_tag="VFO72_03860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9478451.1"
                     /translation="MFGIRKRRPPPFGDVHVDDDKDIQIQKPEQAQRNDLWSEARLLL
                     RDLIFALMLAALVVVFVVQPVKVEGTSMLPRLHDGERIFVNKLIYYDEYRWAPKVERG
                     DIVVFWYPEDPSKSYIKRVIGLPGDTVEIHEGVVRVNGQVMEESYLDQRLNVSHRTIE
                     AVRVRANYYFVMGDNRDNSSDSRIWGLVPKKYIYGKALFRYWPLTSASVIHHEDLAPA
                     GPPAPRSSYTPEPQPEETPAGEPSPERPF"
     gene            5452..6753
                     /locus_tag="VFO72_03865"
     CDS             5452..6753
                     /locus_tag="VFO72_03865"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478452.1"
                     /translation="MTDWLRLLLMIFYAPVRGMRAARDRGSLAPIAFLAFLGQAAYSF
                     VTDKFAGVGGFVGGRIVSELFGAAVVVVVLAVVLVPILTLVANTFERRGSFRVVITQE
                     YTPVAATMFYVLTIANVYSILLAALLHYTGLQHQQVASMIQHADQTRAMLPEGPQFDQ
                     AVEQLKNPSISAQSLFRIPKLVVFSIGTVAGVKDVFRTSAMRALAITVVSAFAATFIA
                     PIAAALFSGVLGSPFLLLLLFLLLRGYFSDIIGTQRAKAAFRQNLESATLNPADASAH
                     YNLGLIHQSRGELDQARERFERALQIDEGEIDASYQLGRIARQQKRYADAIHNFEYVV
                     MRDPAHSQYEIWREVAATYIAAGQFEDARNALEQFLEHRPSDPEGLYLMGRAHAGLGH
                     KREATNLMQACIEAVKTAPAYKYRTSKRWLNEAQQFIKGSQ"
     gene            6768..>7099
                     /locus_tag="VFO72_03870"
     CDS             6768..>7099
                     /locus_tag="VFO72_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HET9478453.1"
                     /translation="MLTKQPALLALEDGRTFRGTSWAAEGEACGEMVFNTSMTGYQEV
                     LTDPSYAGQIVCMTYPLIGNYGVNESDAESSRPWVEGFVVREASRVTSNWRAEESLQS
                     YLKRWNIVA"
BASE COUNT         1572 a         1952 c         2090 g         1485 t
ORIGIN      
        1 ggtcttcggc caacgatgaa ccaaattcca ggcgacggat tatttcagcg cggtcttcct
       61 gattcaggtc tctggcgcgc ttatgttcct gcagggcgtt catctgcatg tccctcgggg
      121 cgtcgaagta agaattctaa agtgaaatcg cctttggcag cgtggttgct tactcaaagt
      181 ggtcctgtgc gggttagttt ctacgctgta aggaaacccg cgcgaccccc gggcttgtgt
      241 actctctcga ctttgaatct tttgacgatg ctagggcgag taccggcgcg aaacggctac
      301 cgagttgcgg cactgtagca tgaagctcat ggccgcgcaa gaggataacg gtccaacata
      361 ttgtttatgg agccaaaagc caacattctc gtcgttgacg ataatccgga caagctgagc
      421 cttctggaag ccgctttatg cctggccggt taccgcgtaa ctaccgctac tgacggcgac
      481 gaagccctct cagccatcga gtcgtaccaa cccgacttgg tgattaccga cgtcatgatg
      541 ccgcgcatga acggctacga attggcccag cgaatccggg ccaatcccgc cacgcggttc
      601 atcccagtaa tcatgcaaac cgccgcgagt cgtcgcgctg aagacctgcg ccgcgcctcc
      661 gaagttgggg ctttgggcta catcactgac ccgactgacc tcgatctgct gttggctcga
      721 acccggacgc tgctcgaatt caaggcatac ctcgatgtat gcgaagaagc cgctttcacc
      781 gatcacctga ccggtttggc gaaccggcgg aggtttgaac ggcaactcga gcgcgaggtc
      841 gggcgcacca tgcgattcgg acatccgttc agtttgttga tggtcgacat cgataatttc
      901 aagaacctca acgacagctt cggccacgac gcgggcgacg acgccatccg ccgcatcagc
      961 aaagtgctgc gggaaggcac gcgcggtatc gatctggcgg ccagaatcgg cggcgaggag
     1021 tttgcggtgt tgttggtgga aactgatcag aggggcgcga ttgacgtcgc cgaacgtttg
     1081 cggattgcaa tcaagtcgct ggagctcacg tctgcgactc agatcaccgc gagcttcggg
     1141 gttgcggaat gtccgattga cgcgcaaact gcaactgaca tcctaaaggc agcggatgtc
     1201 gccttgtatg aggccaaacg ggcgggacgc gatcgagtgg tcggaacgcc cggcatgagg
     1261 tcgaactcgg tggctgccgg ggacgtacaa ggcgatggca atctgggcgt tgcatcgaga
     1321 ggataggtat ggctgattac aaagaaaagc tcggcgagtg gcagcaggcg gcgcggcgaa
     1381 aggctcgcga gctggacgag aagtttgcga tttccgatct tgttggcgac agtgcgcgag
     1441 tggctggtga cgctgcgcgg cgcggcgctg aaacgatggc aagtggcgca gagcgtctgc
     1501 gcgcagaagc cgagcgaatc gccgacgatg aggagatgag tgaaaccgcg cggcgagccg
     1561 ccagtggtgt gaagaaggcc ggtaaagttg tgcgcgacgt ggctggtgac gcgggtaaga
     1621 aggcgggcga agtgtttgac gacgccagga cttactatga ccgcgcgtca cagttctatg
     1681 acactggtgc tcgactcacg cgcgcttcga ctgcggcaac agcgggcgtg ttaaaggcgc
     1741 gtgactgggt taaggagaat ccaggcaagg ctgcggtggt ttcagtttca ctcgtgttgg
     1801 ggattcgcgc cggcgcggcg tttccgggtt tggacgcggt gttgttggga gcgcaacctc
     1861 attggctgac gcattcggcg ttgccggttt acggtttacg caaagcgagt gagaagtttg
     1921 atagctactt gaagaagcgc gaagagctaa tcgcgcgggg cgaggttgac gaagcggagc
     1981 gagagcggat cgaatttgag cggaagatcg cgaagtatgt tggagcgccg ctactcggcg
     2041 cgtttagttg tgccgcgggg gcggcaatgt gggcccagat cttccagcct ggtccactcg
     2101 tcggagcacc gatcagctgg ttgctcggag gcaatccgtt tctggacggc gtgtggctct
     2161 ttgcgaatgg cgtcatctgt ttccaccagg gctacaaatt cttcatgatc gccctcgcga
     2221 accaggaaga agtcgcccgc gtcgtccgcg agatcaaagg cctgctgcct gccgcagccg
     2281 cgagttaaga gtggcttgac tcgttctggt aaacttcgcg tgtgcctcac cgactttgcg
     2341 cctttgcgcg agacaatttt tttcccacag tgttcctcaa acgccatttg cgtgcaaacc
     2401 cggttgaagc ggctttgccc gaaaagccaa tttatttgta cctaagcgtc ttgctccgga
     2461 ggagcaaaat atttatagct cttcggttcc tgaattatgc cgctccgtca ggagcgatat
     2521 gtaaaccgaa cggccagagc acattgcgct ccgctaaagc gcactagtta ataaaaacgt
     2581 aaggctctaa acatcttgct cctccggagc acttctcagc agaaggatca agtaaaaagc
     2641 acttttcgtg caaacccggt tgaaggtttg gcgttggacg gtcaaaagct ggtcggcgtt
     2701 gtgggccgat tccattcgac ctctcgtttt ttgcttatta ctgttttatg aaatccgcga
     2761 agcgcaccaa caaagtcctt acggttacca atgcaaacac tgccaaggga ccgttcggcc
     2821 caaacaagtt gctcgggaag cgttcaagca caagaaccga tttattatcc ttgaaggagt
     2881 cgtagtcgga gtctgcgatg cctgcggcag tcgatattac cgcgccgaga tcctccacgc
     2941 ggtccatgac atcgcaaccg gcgcgaagcc cttcgagcgc gtcgaagaaa tccccgtttc
     3001 gcacctgcct acgcgttggt ccagcctggg tcccgcgacg acgagacacc gagagaaagc
     3061 ctcgatagag ctgtcgtgaa tctcacgtga tcgtgatcgg aaacggccag aagcagagga
     3121 tgaagacgat gagcgtcagg atggcgatgg cgattcgttt cgcgccgagg ggttccatga
     3181 cagcaggcgc cgggtgcggc acgcgcagca tcaccgccaa cagaattgtg tacagaaagc
     3241 cgctcgggct gttgtgccac caaaatccca acagcgcgag aactgccgac gcgacgaacg
     3301 cgacgcgcgc gatcacgtaa tgtgcgcgag gtccaaacac cgcgaacgtt ccatgcccac
     3361 catcgagctg gcccaccggc attaagttca aactcgttac caacaaacca atccatgccg
     3421 ccatgtagta aggagtagtc aacgcgttcg gatcgagcgg cacgccgaac aatttcgcga
     3481 tcaaccgaaa caacagcggg tcgttaaaga agatcgcatg ttccggcggc gccacggatg
     3541 gccccaccgt caacacgccc accaccgaca atggcagcgc aacgacaaat cccgcgagcg
     3601 gtcccgctaa gccgatgtca aacagcgctc gtcgcgaggg aatcggcgac cgaatcttaa
     3661 taaaggcgcc aaatgttccc gccagaaaca acggtggcgc gggaatgaaa tacggcagcg
     3721 tcgcatccac cttgtagtaa cggcacgcga ggtaatgtcc catttcatgc gagaatagaa
     3781 tcgccagcag cgacgctgaa aatgcgacgc cctgcgctat gagtcgaggt tcggccagtc
     3841 cgtatctgat cagttcgacg atggtgtgca ggaaggcgag tggaacgtaa agcaagtaac
     3901 caaagggccc tgagaaaggc gggtcgtacg gcggcagttc aggaacgacc agcaacattc
     3961 cagcgagtgt ggttgtaatg atggtggcga gaaagagcac accgtgcacg cgccactcgc
     4021 gagccgagat cgacaaccca ccggctcgtg gcaaatcaaa accttgcggc actgctcgaa
     4081 acgattcagc cataaacgct ctttagaagc ttaaaggcta gagtctggat gcacttgcgt
     4141 ttccatctct agcctttgaa ctaagactct cttattttcc aaaacactaa aactcgtcgt
     4201 cgtcacctga ttcgccggct tcggtctcgc cgttgtcgtg ggcctgaagc tccttgcccg
     4261 gtttgaagcg aatagtcttg cctgacggaa tcgccacctc ctcgcccgtg cgtgggtttc
     4321 tgccgacgcc acgctttcgt ggtttcacca caaagacgcc aaagcctcgc agttcaatcc
     4381 gctcgccgcg ggtcagcgcc tccttcatcg agtgaaagag agcttccacg gcttgttcgg
     4441 ccttcatctt cggcacgccg gttttgtcgg ccacgcgatt aataatatcg agcttgatca
     4501 cgtctagggc ctcttttctg gtccgactca ggacggatgt ctggatgcaa aatgcaggcg
     4561 aggatgatag agagcgcgac taaaggctgt caaggaaacc ttttccgcgg caagaagctc
     4621 aaaaatactg gtttgacaag agtgatagcg aaagctaagt ttgcgtagcg gccgcgcggt
     4681 ttgccgcgca gtcgacgcga gattctatgt ttggaattag aaaacgccgt ccgccaccgt
     4741 tcggtgacgt gcacgtcgat gacgacaaag acattcagat acagaagccc gagcaggcgc
     4801 agcgaaacga tctgtggtcc gaagcacgcc tgttgttgcg cgatttgatc ttcgcgctga
     4861 tgctcgcagc gctcgtcgta gtcttcgtcg tccagccagt gaaggtggaa ggcacgtcga
     4921 tgctgccgcg gttacacgat ggcgagcgca tcttcgtcaa caaactgatc tactacgacg
     4981 agtatcgttg ggcgcccaaa gtcgaacgcg gcgacatcgt tgttttctgg tatcccgaag
     5041 atccttcaaa gagctacatc aaacgcgtga ttggcttgcc gggcgacacg gtagaaatcc
     5101 atgaaggcgt ggtgcgcgtc aacggccagg taatggaaga gagctatctc gaccagcggc
     5161 tcaatgtgtc gcatcgcact atcgaggcgg ttcgggtgcg cgcgaactac tatttcgtga
     5221 tgggcgacaa tcgcgacaac tcgagcgatt cgcgaatctg gggtcttgtg cccaagaagt
     5281 acatctacgg caaagcactg tttcgttatt ggccgctgac gtccgccagc gtgattcatc
     5341 acgaggatct cgcgccggca ggtccgcctg cgccaaggtc gagttacaca cctgaacctc
     5401 aacctgagga aacaccggcg ggcgaaccga gcccggaacg gccgttttga tatgactgac
     5461 tggctgcggc tcctgctaat gatcttttac gcccccgtgc gcgggatgcg tgcggcgcgt
     5521 gaccgtggct cacttgcgcc cattgctttc ctggcgtttc tcggccaggc agcgtacagc
     5581 tttgtcacag acaagtttgc cggtgtgggt ggtttcgtag gcggcagaat cgtctccgaa
     5641 ctttttggcg ccgcggtcgt ggtcgtggtg ttggcagttg tgcttgtgcc gatacttacc
     5701 ctggttgcga acacattcga gcgccgcggc agttttcggg tggtgatcac gcaggagtac
     5761 acgcccgttg cagcaacgat gttctatgtg ttgactatcg cgaacgtcta ttcgattctc
     5821 ctcgcagcgt tgcttcacta cacgggtctc cagcaccaac aggttgcaag catgattcag
     5881 cacgcggatc aaacgcgcgc catgctgccc gaagggccac agtttgacca ggcagtggaa
     5941 caactcaaga acccgtcgat ttcagcgcaa agcttgttcc ggatcccaaa actggttgtg
     6001 ttctctatcg gcacggttgc cggagtgaaa gacgtctttc gcacctccgc gatgcgcgcg
     6061 ctggcgatca ctgttgtttc agcttttgcg gcgacgttta tagcgccgat tgcagcggcc
     6121 ctgttctcgg gcgtgctcgg ctcgccgttt ctactgttac tgctattcct gttgctgcgc
     6181 ggctacttca gcgacatcat cggaacgcaa cgagcaaaag cggcattcag gcaaaacctc
     6241 gaatcggcaa cactgaaccc cgcggacgct tcagcgcatt acaatctcgg attgattcat
     6301 cagagccgcg gcgaactcga tcaggcgcgc gaacgtttcg aacgcgcgct acagatcgat
     6361 gaaggcgaga tcgatgccag ttatcaattg ggtcgtattg cacgacaaca gaagcgttac
     6421 gccgatgcga ttcataactt cgaatacgtc gtgatgcgcg atccggcgca ctcacaatac
     6481 gagatctggc gcgaagtggc cgcgacttac atcgcggcgg gccagtttga agacgcacga
     6541 aacgcgctcg agcagtttct cgaacaccgg ccgtccgatc ccgaaggcct ctatctgatg
     6601 ggtcgcgcgc acgcgggcct cggccacaaa cgcgaagcta ccaacctcat gcaggcgtgt
     6661 atcgaagccg tgaaaaccgc gcccgcttac aagtatcgaa cgagcaagcg ctggctcaac
     6721 gaagcgcagc agtttattaa aggtagccag tagacggggt aaaagaaatg ctcactaaac
     6781 aacccgccct cttagcgctc gaagatggtc gtacgtttcg cggcacctca tgggctgctg
     6841 aaggtgaggc ctgcggcgaa atggttttca acacgtcgat gaccggttac caggaagtgc
     6901 tgaccgatcc ttcgtacgcc ggtcagatcg tctgcatgac ctatccgctg atcggtaact
     6961 atggtgtcaa cgagagtgac gcagaatctt cgcgcccgtg ggtagaaggt tttgttgttc
     7021 gtgaggcgag cagagttacg tccaactggc gcgcagaaga aagtctgcag tcgtatctca
     7081 agcgttggaa catcgttgc
//
LOCUS       DASTEY010000215         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_268436, whole genome shotgun sequence.
ACCESSION   DASTEY010000215 DASTEY010000000
VERSION     DASTEY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268436"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFO72_03875"
     CDS             complement(<1..605)
                     /locus_tag="VFO72_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478454.1"
                     /translation="MSELSHETRRQIGALVDRQGHIQYVMVGDNRRIELPDFKRVRVA
                     SGRFRGLRCVHTHLRGEPLTQDDLTDLALLRLDLMAAIDVDAESGLPGLVRAAHLLPL
                     HAGELDNATTPDLYAFLEPKIASQLDLDFQALIDSLEEEMARNRRTARRAEVRDRTIL
                     VGVATGSLADAEESMAELAELAASAGVVVQDEIIQRRTAIDP"
     gene            832..1860
                     /locus_tag="VFO72_03880"
     CDS             832..1860
                     /locus_tag="VFO72_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET9478455.1"
                     /translation="MATAAKILNIDPAYGIAGGEIVIDCEGFDTSDPSRCGVTIGEAQ
                     AQIVALGPRRVLAVVPETKGGKVEVRLSSNGELSEPAFLTVAKKLAGDLHPVANPAFD
                     PDDGALFVTRSGSRGEELPVTLFRIDISGDVSEYSGDISNPTGIAFSPDGQMFVTSRL
                     DGNVYKVNPFKEAVAFARNLGFATGIAFDSQGLMHVGDRSGTIFKVNGIGEERVWTEI
                     EGSVSAFHLAFGPDDSLYITGPTLTSFDSIWRINPEGEVDVFFKGLGRPQGLAFDREG
                     NLYVAAALRGRRGIVRISPGGTEAKVVVAGVNLVGLAFSATGEMAVVSIDSVYSLPTQ
                     IKGTLLPG"
BASE COUNT          408 a          546 c          662 g          506 t
ORIGIN      
        1 ggatcgattg ccgtgcgacg ttgaatgatc tcgtcctgga ccacgactcc tgccgatgcg
       61 gcgagctccg cgagctccgc cattgactct tcggcatccg cgagtgatcc ggtggccacg
      121 ccaaccagga tcgtgcggtc gcgcacttcc gcgcggcgcg ccgtgcgacg attgcgcgcc
      181 atctcttctt cgagtgagtc gatgagggcc tggaaatcga ggtcgagttg cgaagcgatt
      241 ttgggttcca ggaaagcgta gaggtcggga gtcgtggcgt tgtcgagttc gcctgcatgc
      301 agcggtaaca aatgcgccgc gcgcacgaga ccaggcagcc ccgactcggc atcgacgtcg
      361 atggcagcca tcaagtcgag gcgcagcagg gcaaggtccg ttaagtcgtc ctgcgtgagt
      421 ggttcgccgc gcaggtgcgt gtgcacacaa cgcagaccgc gaaaccttcc cgaagcgacg
      481 cgaacgcgtt tgaagtcagg tagctcaatg cgacggttgt cgccgaccat cacgtactgg
      541 atatgtccct ggcgatcgac gagggctccg atctgtctgc gtgtttcgtg agacagttcg
      601 ctcaactggc gggcaaattc ctgtgtgacg atctggttgg gaggtatgcg ccgagtgtac
      661 agcttttcta tgcgccgcag ttgatttggt tttaacccca gagtattgcc cagaacgctg
      721 ctaatggaag tatcctctca gggggcggat tataacatag tgctgccagc tacttcccag
      781 attcaaggct tttgtgctta ttaacggctg gcagtattat gaacgacttt tatggctact
      841 gccgcgaaaa ttctcaatat tgatcctgct tacggcatcg cggggggcga gatcgtgatc
      901 gactgtgagg gctttgatac cagcgatccg tctcgttgcg gggtcacgat tggagaggcg
      961 caggctcaga ttgttgccct tggcccgcgc cgagtattgg cagtggtgcc cgagacaaaa
     1021 ggcggcaagg ttgaagtcag gctcagcagc aacggcgagt tgagtgagcc cgcgtttctc
     1081 acggttgcaa agaaactcgc cggcgatctt catcctgtcg ccaatcctgc attcgaccct
     1141 gacgatggcg cgctctttgt cacgcgttcc ggatcgcggg gcgaggaact gccggtcacg
     1201 ctgtttcgca tcgatatcag tggcgacgtt tccgagtact cgggcgacat ctcgaatcca
     1261 accggcattg cgttcagtcc cgacgggcag atgttcgtca ccagcaggct cgacgggaat
     1321 gtttacaagg tgaatccgtt caaagaagcg gttgcgttcg cacgcaatct gggcttcgcg
     1381 accgggattg ctttcgatag ccagggtttg atgcacgtcg gcgatcgctc gggaacgatc
     1441 tttaaagtga acggcatcgg tgaggagcgt gtgtggacgg agattgaggg atcagtttcg
     1501 gcgtttcatt tggcgtttgg gccggatgac tcgctgtata tcactgggcc aacgcttacg
     1561 agcttcgatt cgatctggcg tataaaccct gaaggtgagg tggatgtatt tttcaaggga
     1621 ctagggcggc ctcagggact cgcgtttgat cgagagggga acttgtacgt tgccgcggcg
     1681 ctgcgtggcc ggcgcgggat cgttcgcatc tctccgggtg gcacggaggc gaaagtggtt
     1741 gtggcgggcg tgaacctggt tgggcttgcg ttcagcgcta ccggagagat ggcagtcgtt
     1801 tcgatcgatt cggtctacag tctacctacg cagatcaagg gaaccctctt gcctggttaa
     1861 gaattaacca aaagacacgc aggcacaact ttattttcaa ctttgtgtct tagtgccttt
     1921 gtggttagac tactctctgc tttgcatctt gttagtgcga gtgtatctgt ttgagtccgg
     1981 aacaactgat tcctcaactc gtgatttata tggtcgtgct gctgcttgca atctcggcac
     2041 acgaggcagg gcacgcctgg atgtcttaca agtttggaga tgacacggcg tatttgctgg
     2101 gccgcgtcac gctcaacccg gt
//
LOCUS       DASTEY010000216         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1323065, whole genome shotgun sequence.
ACCESSION   DASTEY010000216 DASTEY010000000
VERSION     DASTEY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323065"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFO72_03885"
     CDS             complement(<1..236)
                     /locus_tag="VFO72_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478456.1"
                     /translation="MRLVALSKNRGAFRKSRRVSKIGLAGLLLLVVSTVVTAQEEAPP
                     PWARFEPEGGGFSILMPGKPQETITKRPAFTLHS"
     gene            complement(255..2120)
                     /locus_tag="VFO72_03890"
     CDS             complement(255..2120)
                     /locus_tag="VFO72_03890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET9478457.1"
                     /translation="MPSLSDAITTLREHFGFDDFREGQREVVGSILEGKDAVVVMPTG
                     SGKSLCYQLPAMILDGVTLVVSPLIALMKDQVDALQARGLPATFINSSLAESEQRARI
                     DALRRRVHKLVYIAPERFRSSRFVSAVQSIPISLFAIDEAHCISMWGHDFRPDYLRLK
                     HVIRSLGRIQTVALTATATPYVRSDIIQQLGLSQPETFISGFDRPNLSIEVVHTEKEK
                     EKISRIRSLAQKTGGSGIVYASTRKAVEQVGRELKNAGLKVSTYHAGMSDGVRIKEQD
                     DFMNGRTQMIVATNAFGMGIDKPDIRFVAHYQIPGSIEAYYQEIGRAGRDGLPSTCVL
                     LFNYADKNTHDFFIEGSYPDISTVKNVYGALLGTGLKRIELTTAQIAKMASEKNEMAV
                     QSALYLLERAGHISREAPVARGARAPRAIVMLDNAPIASLRVDPRQIAGRADLERRKL
                     RELINLCYTDYCYRAHILNYFGDRSHERQCGTCGNCRPKTTAPVHEIAFDESPPPAVV
                     ANSEPRALTDDELLRVRKILACAKRMNGRFGKKLLAATLRGSASKQVMSAGLNELSTY
                     GLLKDMLQDEIETYVDALITTRCLKVKAGAYPTVSTTELGDRVMREQEKIELALP"
     gene            complement(2056..>2824)
                     /locus_tag="VFO72_03895"
     CDS             complement(2056..>2824)
                     /locus_tag="VFO72_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9478458.1"
                     /translation="PASLSGAGTANLSINADGVTSNPVSVLIAGAPPVSSDKLVITQL
                     FGGGGNSGAPFRNDFIEIFNAGGTPMNLAGWSVQYASATASTWSVTPLTNVALSPGQR
                     YLIQQAGGNNGATLPTPDATGTTAMAAGAGKVALVKSTTALTGSCPADPNIVDFVGYG
                     ATANCFKGAGPAPAASNTNAATRVGNGCTDTRNNATDFALAPPNPRNTAAPLNSCALV
                     QSVLLCPRRVETFSIAHALAQRRNHNFARALRLRRFP"
BASE COUNT          543 a          768 c          861 g          652 t
ORIGIN      
        1 taggaatgta gtgtgaaggc tggccgtttc gtgatggtct cctgtggttt gccgggcatc
       61 aggatggaga agccgccgcc ttccggttcg aacctggccc acggtggagg tgcttcctcc
      121 tgcgcagtca cgaccgtcga aacgaccaac aacagaagac cggcaagacc aattttagaa
      181 acgcgtcgac tctttctgaa tgcgccacga tttttgctaa gtgcgactag tctcatagcc
      241 cgaacccatt cgcctcacgg cagagccaat tcaattttct cctgttcgcg catcacgcga
      301 tcgccgagct cggtggtgct cacggtcggg taagcgcccg cttttacctt caggcaccgc
      361 gttgtgatta gcgcatcgac gtaagtctcg atctcgtcct ggagcatgtc cttaagtaaa
      421 ccataagtcg agagttcgtt gagcccagcg ctcatcacct gcttcgaagc tgagccgcgc
      481 aacgtcgctg cgagcagctt ctttccaaag cgcccattca ttcgtttagc acacgcgaga
      541 atttttctca cgcggagcag ctcgtcatcc gtgagagcgc ggggttctga gtttgcgacg
      601 acggctggtg gtgggctttc atcaaatgcg atctcgtgaa ccggtgcagt ggtctttggg
      661 cggcagttgc cgcaagtgcc gcattgccgt tcatgactgc ggtcgccgaa gtagttgagg
      721 atgtgggcgc gataacagta atcggtgtag cagagattga tcagttcgcg cagcttgcgc
      781 cgttccaagt ctgcacggcc agcgatctgt ctgggatcga cccgaagcga cgctatcggc
      841 gcgttgtcga gcatgacgat cgcgcgcggt gcacgggcgc cgcgtgcgac cggcgcttct
      901 cgcgagatat gtccggcccg ttccagtaga taaagcgcgg attggaccgc catctcattc
      961 ttttcacttg ccatcttggc gatctgcgcc gtggtcaact cgattctctt caaaccggtt
     1021 ccgagtagcg ctccgtaaac gttcttcacc gtgctgatgt cgggatacga gccctcgata
     1081 aagaagtcgt gcgtgttttt gtcggcgtaa ttgaacagca acacgcaggt tgacggcagt
     1141 ccatcgcgtc cggcgcgtcc gatctcctgg taataggcct cgatcgatcc gggaatctgg
     1201 tagtgggcca cgaagcggat gtcgggcttg tcgatcccca tgccgaacgc atttgtcgcc
     1261 acgatcatct gcgttcgacc gttcatgaag tcgtcctgct ccttgatgcg gacgccgtca
     1321 ctcatgcccg cgtgataggt cgaaactttt agaccagcgt ttttgagttc tcgtccgacc
     1381 tgttcgacgg ctttgcgagt cgaggcgtag acgattccgg aaccgccggt cttttgggcc
     1441 aggctacgaa tgcggctgat cttctctttc tctttttccg tgtggacgac ttcgattgag
     1501 agattgggcc ggtcgaagcc gcttatgaag gtctccggtt gcgagagtcc cagttgttga
     1561 atgatgtcgg aacggacata aggcgtcgct gttgcggtga gagcgacggt ctgaattcgt
     1621 cccagcgagc ggatgacgtg tttgagtcga agatagtcgg gacgaaagtc atgtccccac
     1681 atcgaaatgc agtgcgcttc gtcgattgcg aacagtgaga tgggaatcga ctgcaccgcc
     1741 gagacgaacc tgctgctgcg aaagcgttcc ggggcgatgt aaacgagttt gtgtacgcga
     1801 cggcgcagcg cgtcgatgcg cgcgcgttgc tccgactcgg cgagcgaact gtttatgaaa
     1861 gtggcgggca ggccgcgcgc ctgaagtgcg tcgacctgat ccttcatcag cgcgatcagc
     1921 ggcgacacta ccagcgtgac tccgtcgagg atcattgccg gaagctggta gcagagagac
     1981 ttcccgcttc ccgtcggcat cactacgacg gcgtctttgc cttccaggat tgatcccacg
     2041 acttcgcgct ggccttcacg gaaatcgtcg aagccgaagt gctcgcgcaa agttgtgatt
     2101 gcgtcgctga gcgagggcat gggcgatact aaaggtttcc actcgtcgcg gacaaagtaa
     2161 cacggattga accaacgcac atgaatttaa aggcgcagca gtgtttcgcg gatttggcgg
     2221 tgcgagtgcg aagtcggttg cgttgttgcg cgtgtcagtg catccgttgc caacgcgagt
     2281 agcggcattg gtgttactag ccgccggtgc cggccctgca cctttgaagc agtttgcagt
     2341 tgctccgtag ccgacaaagt caacgatgtt tggatcggcg ggacaactgc cggttaaagc
     2401 ggtagttgat ttaacgaggg cgactttgcc ggcgccggcg gccatcgcgg tagttcccgt
     2461 tgcatctgga gttggcagag tggcgccgtt atttccgccg gcctgctgaa tcagatagcg
     2521 ctgaccgggt gatagggcga cgtttgtcag cggcgtcacg gaccaagtgg acgcggttgc
     2581 actcgcgtac tgcactgacc agccggcgag attcatagga gtgccgccgg cgttgaagat
     2641 ctcgataaag tcgttgcgaa aaggagcgcc ggagtttccg ccaccgccga ataactgtgt
     2701 gattacaagt ttgtcagatg agaccggcgg tgctcccgca atcaacacgg ataccggatt
     2761 gctggtgacg ccatcggcgt tgatgcttaa gtttgctgtg cctgctccac tcagactggc
     2821 gggt
//
LOCUS       DASTEY010000217         5934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_268472, whole genome shotgun sequence.
ACCESSION   DASTEY010000217 DASTEY010000000
VERSION     DASTEY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268472"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..2534
                     /locus_tag="VFO72_03900"
     CDS             357..2534
                     /locus_tag="VFO72_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9478459.1"
                     /translation="MINLISIYLNVCLAFSFAMANDWAEWRGPARDGISTEKNLPDKW
                     SPAGENLAWRAPYGGRSAPIVMADRVYLVNSAGSGETLQERIICINADTGQLLWEHRF
                     NVYLSDVPPHRVGWASPVGDPATGNVYVFGVGGKLIGLNRDGKVLWERSLGEDFGLLT
                     THGGRTTSPIIDGDLVIVSGVTFQWGQHGRGAHRFMAFDKKTGETVYVSAPGGRPYDT
                     TYAPPIIVNINGTRLLIQGASDGVVHAIKPQTGEPVWKHEISKRGLNTGVVVKGTTAI
                     LTHSEENLDSNEMGMMVAVDATAKGDVKKEQVKWTVYGWQGGFSSPVIDGDRVYQVDN
                     GANIAAFDVNSGKQLWIQNLGTIQKASPVLADGKLYVGTENGKFFILKPGPTSAEILD
                     QDQLGTETRPEAIIASAAVSNGRIFVVSDSALYAIGKKATSSSQTSTNASGSNVSSNP
                     ATHVQVIPTELILKPGDKVSFRARLFDAQGNFIREETSAQWSLDQLKGTIANGQFTAA
                     GDTAQAGLVKATVNNISGTARVRIFPPLPWSENFDAIAPNTLPPTWVNTTMKFAVRDQ
                     NGNKVLAKLTEGSSLLSRARAYMGPSDWSNYTVEADVSATTKRRQRGDAGVIAQRYVL
                     SLYGNSQMLHLEPWQPETQRTVTKPYAWKPDTWYRLKLQVENLPDGKTRARGKVWPAG
                     EAEPAAWMIERIDPIPNRQGAPGIFGNGLAELYFDNIKVYANK"
     gene            2531..4003
                     /locus_tag="VFO72_03905"
     CDS             2531..4003
                     /locus_tag="VFO72_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9478460.1"
                     /translation="MRVKNLFLVIALLLGAGIWFLAPTTAASDPGSGDWPMWGGTPDR
                     NMISNMKGLPISWDVKSKKNVKWIAQLGSQTYGNVVVSAGMLFVGTNNEGLRDPKITG
                     DKGILMAFRESDGEFMWQMVHDKLAAGRVNDWPYQGVASSPYVEGDRVYYVSNRAELI
                     CLDTQGFRDKENDGPVTDETLTGEFNADIVWRFDMIEEVGSLPHNLANSSPVAHGDLI
                     FVSTSNGQDESHVNVPSPKAPSIIAVNKKTGKLAWEDNSVGDRILHGQWSSPTVGTVG
                     GVVQVVHGQGDGWIRGYEAATGKKLWEFDTNPKDSVWPKTRNELISTPVIYQDHVYIA
                     NGQDPEHGEGVGHMYCIDATKRGDITKTGLVWHYDKIRRSISTPAIKDGIVYQPDFSG
                     FLHALDAKTGQVYWVHDLFAAVWGSPLLVDGKLYLGDEDGDVVIMAEGKEKKVLAEIE
                     MGSSVYSTPVPANGVLYISNRNQLYALSEAAAAKPATARN"
     gene            4009..5157
                     /locus_tag="VFO72_03910"
     CDS             4009..5157
                     /locus_tag="VFO72_03910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9478461.1"
                     /translation="MKSFDFQPRTRLVFGAGAIGQLGNVAQELGLRRTLLVADHGLVA
                     SGHVDEAVTPLEEAGIEVIRFHDFEVNPDTRMLESGTAFVATQQIDSIIGLGGGSSMD
                     CAKGINFLLTNGGRMADYRGYGKATKPMLPMIAIPTTAGTGSEAQSYALISEADTHVK
                     MACGDPKAAFRVALLDPALTLSQPRSITATSGFDAIAHAVETYVTTKRTPVSEIFSRE
                     AWQLLERNYERVLAQPDDLEARGAMQLGAFYAGMAIENSMLGATHACANPLTARYGTA
                     HGAAIAMLLPSVVRWNERAAADRYATLLSFSSVAGKTTDLTPTERLARRLEELTEAGG
                     LRVELRAAGIVRNDLAELAAEAAEQWTGTFNPRPFSKEGAMEVYSCAF"
     gene            5145..>5934
                     /locus_tag="VFO72_03915"
     CDS             5145..>5934
                     /locus_tag="VFO72_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9478462.1"
                     /translation="MRVLRVICLVLSVVLVANAQDAPGNNWSQFRGNHSLTGVSQSNV
                     PSSLRQLWTYEAGESIESSAAIVGGTVFVGSHKGELVALSLDNGSVYWKFSTGNPIGE
                     SSPAYSNGVVYIGDLAGWINAINATDGQKLWAFKTNNEIKSSPVVVGDRVMIGSYDEH
                     LYCLSARNGSLLWKFKTNGPVHATPGIADGMAFIAGCDEMFRAIRISDGKEVFNVSSG
                     AYTGASPALRAASAFYGTFDNEVLGVDLAQRKIAWRYEHPQRKFP"
BASE COUNT         1342 a         1669 c         1713 g         1210 t
ORIGIN      
        1 ctggggacac ggtcggaaat acagccgaac gctgaaagcg ttggccagca cttcgcaact
       61 gctttcgcca acgctttcag cgttcttggt tctttcattc ctgattccca gggttgaagc
      121 aaccccgggc tggaattagc caacgccttc ggcgtaacca ttacgcggtg ctcaactcaa
      181 acagacccac tactcacaga cccactactc aaaatccgcg tcaggttaac ggatgagacg
      241 gaacctgtta gaatcatcgg aaaaaagtgt aacgcagcaa cgtgagctta cgtattttac
      301 atccttaggg ttgcaagcga aacatagttc atcctgaacc gagacggttt tctaaaatga
      361 ttaacttgat ctccatttat ttgaacgtct gccttgcatt ctcgtttgca atggcgaacg
      421 attgggccga gtggcgtggt ccagcgcgcg atggcatctc gacggagaag aacctgccgg
      481 acaaatggtc acccgctggc gagaacctcg catggagggc tccgtacggc ggtcgctccg
      541 cgccgatcgt gatggctgac cgcgtctacc tggtaaactc cgcgggatca ggcgagactt
      601 tgcaggaacg gatcatatgt atcaacgcgg ataccggaca actgctttgg gaacaccgtt
      661 tcaacgtcta cctgagcgac gtaccgccac atcgcgttgg ttgggcttca cctgttggcg
      721 atccggcgac gggaaatgtt tacgtgtttg gcgtcggcgg caaactgatc ggattaaacc
      781 gcgacggcaa ggtcttgtgg gaacgctcgc taggcgagga ttttggttta ctcaccacgc
      841 acggtggccg caccacctcg ccaatcatcg atggcgatct cgtgatcgtc agcggcgtaa
      901 cgtttcagtg gggacaacat ggacgtggcg cgcatcgctt catggctttc gacaagaaga
      961 cgggtgagac tgtttatgtg agtgcgcccg gtggccgccc ttacgacact acttacgcgc
     1021 cgcctatcat cgttaacatc aacggcacac ggctgctgat ccagggcgcc agcgacggcg
     1081 tggtccatgc gatcaaacct cagaccggtg agccggtctg gaagcatgag atcagcaaac
     1141 gcggattaaa caccggcgta gtcgttaaag gcactactgc gattctcacg cacagcgaag
     1201 agaatctcga ctcgaacgag atgggaatga tggtcgcggt agacgcaact gccaaaggcg
     1261 acgttaagaa agaacaggtc aagtggactg tttacggctg gcaaggcggt ttctcttcgc
     1321 cggtaatcga tggtgaccgc gtttatcagg tggacaacgg cgccaacatc gctgcttttg
     1381 acgtaaacag tggtaagcaa ctctggatac agaatctcgg cacgattcag aaggcgtcac
     1441 ccgttttggc cgacggcaag ctgtacgtcg gcactgagaa cggaaagttc ttcatattga
     1501 aacctggtcc aacaagcgcc gagattctcg atcaggatca actcggcact gaaacgcgtc
     1561 cggaggcaat catcgcctcg gcagcagttt cgaatggacg gatttttgta gtcagcgatt
     1621 cggcccttta tgccatcggc aaaaaggcga ccagctcgtc ccaaacttcg accaacgcca
     1681 gcggcagcaa cgtcagttca aatcctgcga ctcacgttca ggtaattccg acagaattga
     1741 ttttaaaacc tggcgataaa gtcagcttcc gcgcacgctt gttcgacgct caaggcaatt
     1801 tcattcgcga ggaaacctcg gcccagtggt cgctcgatca attgaaaggt acgattgcga
     1861 atggacagtt caccgcagca ggcgatactg cgcaggctgg tcttgtgaaa gcgacagtga
     1921 acaacatcag cggcacggcc agggtgcgca tcttcccgcc gctgccgtgg agcgagaact
     1981 tcgacgcaat agcgccgaac acgctgccgc cgacgtgggt caatacgacg atgaagtttg
     2041 ccgtgcgcga tcagaacgga aacaaagtgc tggcgaagct gactgaaggt tcgtcgctac
     2101 tgagtcgcgc ccgtgcttac atgggaccgt ctgactggtc caactacacg gttgaagcgg
     2161 acgtctcagc aacgaccaag cgtcgccagc ggggcgacgc gggcgtgatt gcccagcgtt
     2221 acgtgttgtc actttacggc aacagccaga tgcttcatct ggaaccgtgg caaccggaaa
     2281 cccaacgcac cgtgacaaag ccatacgcgt ggaaacccga cacgtggtac cggctgaaac
     2341 ttcaggtcga gaacctgccg gacggcaaga cacgcgcgcg tggaaaagtt tggcccgcgg
     2401 gggaggcgga acccgcagcc tggatgatcg aacgcatcga tcccattcct aaccgccagg
     2461 gagctcccgg aatcttcggc aacggcctcg ccgaactcta ctttgacaac ataaaggtgt
     2521 acgcgaacaa atgagagtta aaaatctgtt cctggttatc gcactacttc tcggcgccgg
     2581 catttggttt ctcgcgccca cgaccgctgc ttcagatccc ggctccggcg attggcccat
     2641 gtggggcggc actcccgatc gcaacatgat ctccaacatg aaaggcttgc cgattagttg
     2701 ggacgtcaaa agcaaaaaga acgtgaagtg gatcgcccaa ctcggttcac aaacctacgg
     2761 caacgtcgtc gtctcggctg gcatgctctt cgtcggcacc aacaacgaag gactgcgcga
     2821 tccgaagatt accggcgaca aaggtatctt gatggcgttt cgcgagtcgg acggcgagtt
     2881 tatgtggcag atggtccacg acaagcttgc ggcaggacgc gtgaacgatt ggccctacca
     2941 gggcgtggct tcgtcgcctt acgttgaagg agatcgggtt tactacgtct ccaatcgcgc
     3001 ggagttgatt tgtctcgaca cgcagggttt ccgcgacaaa gagaacgatg gaccggtgac
     3061 tgacgaaaca ctgacgggag agttcaatgc cgacattgtt tggcgtttcg acatgatcga
     3121 ggaagtcggg tcgctcccgc acaacctcgc gaattcgtct cccgtcgcgc acggcgatct
     3181 gatcttcgtc agcacttcaa acggccagga cgaaagccac gtcaacgttc cttcgcccaa
     3241 agctccgtcg atcatcgcgg ttaacaagaa gaccggcaaa ctcgcctggg aagacaactc
     3301 cgtcggcgat cgaatcctgc acggccagtg gtcttcgccg accgtgggaa cagtcggcgg
     3361 cgtggtccag gtggtgcatg gccagggcga cggttggatt cgtggttacg aagcagccac
     3421 gggaaagaaa ctgtgggagt ttgatactaa cccgaaagat tctgtctggc ccaagacccg
     3481 taacgaactg atcagtacgc ctgtgatcta ccaggatcac gtttacatcg ccaacggcca
     3541 ggatccggag cacggcgaag gcgtgggcca catgtactgt atcgacgcga ccaagcgcgg
     3601 cgacatcacg aaaacagggc tcgtctggca ctacgacaag attcggcgtt caatttcgac
     3661 cccggccatc aaggatggca ttgtctatca acccgacttc agcggcttcc ttcatgcgct
     3721 cgacgcgaaa acaggccagg tttactgggt ccacgatctg ttcgccgccg tttggggttc
     3781 gccgctgctg gttgacggca agctctatct cggcgacgaa gatggcgacg ttgtgatcat
     3841 ggcggaagga aaagagaaga aggtcctggc tgagatcgaa atgggtagct cggtctactc
     3901 gactcccgtt ccggccaacg gcgtactgta catctctaat cgaaaccagc tttacgcgtt
     3961 gagcgaagca gcagcggcga aaccagccac agcacggaat tagccgcgat gaagagcttt
     4021 gattttcagc cgcggacgcg cctggttttt ggcgcgggtg cgattggcca actcggcaac
     4081 gtcgcgcagg agctcgggct ccgccgcacg ctgctggtcg cggatcatgg actcgtcgct
     4141 tcaggccacg tcgacgaagc cgtcacgccc ctcgaggaag ccggcatcga agtcattcgt
     4201 tttcacgact tcgaagtcaa tccggatacg cgcatgcttg aatcgggaac tgcttttgtc
     4261 gcgacgcaac agatcgactc aattatcgga ctcggtggcg ggagttcgat ggattgcgct
     4321 aaaggcatca acttcctgct caccaacggc ggccgcatgg ccgactaccg cggctacggc
     4381 aaggcaacaa aaccgatgtt gccgatgatc gccattccca ccaccgcggg caccggcagt
     4441 gaagcacaaa gttacgcgct gatctctgag gcagacacac acgtcaagat ggcgtgcggc
     4501 gatccgaaag ctgcttttcg cgtggcgctg ctcgatcctg ctttaactct gtcgcaaccg
     4561 cggagcatca cggcaacttc cggcttcgac gcgatcgcgc acgcggtgga aacttacgtc
     4621 accaccaaac gcacgcctgt ctcggaaatc ttttctcgtg aagcctggca actgctcgag
     4681 cgcaactacg aacgagtgct ggcccagccg gatgatttgg aagcgcgcgg cgcgatgcag
     4741 ttgggtgcgt tctatgcggg catggccatt gagaattcaa tgctcggtgc gacgcacgcg
     4801 tgtgcgaacc cgttaacggc gcgttacgga acggcgcacg gcgcggcgat cgccatgttg
     4861 ttgccgtcag tggtgcgttg gaacgaacgt gcggccgcag atcggtacgc gacgttgttg
     4921 agcttctcga gtgttgccgg taaaaccacg gatttgacac cgacggaaag actggcccgt
     4981 cgactggagg aactgaccga agcgggcgga ttgcgtgtgg agttgcgcgc cgcgggcatc
     5041 gtccggaatg atctcgcaga acttgcggct gaagcagccg agcaatggac cggaactttc
     5101 aatccccgtc cgttcagcaa agaaggcgcg atggaggttt attcatgcgc gttttgagag
     5161 ttatctgtct ggttttatcc gtggtcctgg tcgccaacgc gcaggacgcg cccggaaata
     5221 actggtcgca atttcgcggc aatcattcgt taaccggcgt ctcgcagtcg aacgtcccat
     5281 cctcactcag gcaactgtgg acctatgaag ccggcgaatc gattgaatcg tcggcagcga
     5341 tcgtcggcgg cacagttttc gtcggatcgc acaagggcga actcgttgcc ctcagtctcg
     5401 acaacggatc cgtttactgg aaattctcga ccgggaatcc catcggtgag tcgtcgccgg
     5461 cttatagcaa cggcgtcgtt tacatcggcg acctcgccgg ctggatcaac gcaataaatg
     5521 ccaccgacgg ccagaagctc tgggctttca aaaccaacaa cgagatcaaa tcgtctccgg
     5581 tcgtggtcgg cgaccgcgta atgatcggtt cctacgacga acatctttat tgtctctcag
     5641 cacgtaacgg ttccctgctc tggaagttca aaaccaacgg accagtgcat gcgactccgg
     5701 gcattgctga cggcatggcg tttattgccg gttgcgacga aatgtttcgc gcgattcgga
     5761 tctcggacgg caaggaagtc ttcaacgttt cttcaggtgc ctacacggga gcgtcgccgg
     5821 cgctgcgagc ggcttccgcg ttttatggca ccttcgataa cgaagtgttg ggcgtggatc
     5881 tcgcgcaacg taagatcgcg tggcgctacg agcatccgca acgcaagttt ccct
//
LOCUS       DASTEY010000218         5062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1537387, whole genome shotgun sequence.
ACCESSION   DASTEY010000218 DASTEY010000000
VERSION     DASTEY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537387"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFO72_03920"
     CDS             complement(<1..784)
                     /locus_tag="VFO72_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9478463.1"
                     /translation="MTKSLCYISRVEVAQQFKKYRKKAIAWKFAITATAVILVAATLG
                     GTIGSARRFSSLTSIGAKHSYRVADDIERDYNEAVATITSNYSGEIDHEKATQAAIQG
                     MLSTLDPHSAYFPYSEFKKLKEDQDSRFYGIGVTIVSHRDGVYVQSAVEGTPAAKLGL
                     KYGDRILEVDGKDARDWSSEQVSKNVRGALGEPVRLKVERAGSEAPQYFTIVREAVPL
                     LTIRNAYMIRPGTGYIGLTGGFQRSSDEELANAMKRLEEQGMR"
     gene            931..1701
                     /locus_tag="VFO72_03925"
     CDS             931..1701
                     /locus_tag="VFO72_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HET9478464.1"
                     /translation="MSASLSIVIPAYNEAVRLGNTLPAVVNYLRESWPDAELIVVDDG
                     SRDDTAALARAIFKNNAGLRTSVISYKSNLGKGRAVRLGLLAARGDVALFSDADLSTP
                     ITEAPKLIEPIVSGQYDVTFGSRALDRRLIGVHQPWRREQGGRVFNLVVRLATGLPFW
                     DTQCGFKAFRMSVCRPLVEAATVDRFGFDVELLYLAFRAGLRLKEVPVRWDHNEGSKV
                     SVMSDSFRMLSEVGLIRQQARRGVYDSAIRAVHELTSA"
     gene            1784..2548
                     /locus_tag="VFO72_03930"
     CDS             1784..2548
                     /locus_tag="VFO72_03930"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET9478465.1"
                     /translation="MTARLNINIDHVATVRQARRAPEPNLIAAAMACEQAGADGITVH
                     LRGDRRHIQDLDIRLLRDSIITYLNLEMAATDEMLDIALQTGPDAVTLVAERPNEITT
                     EGGLDVQANVAVVKAAVDRLREASILPSLFIDPDPAQIEVAHQIGAQQVELCTAAYAE
                     ATLGARGIHGEGAARAARELQRLKEGAELALRKGLNVAAGHGLTYRNVGALARISEIT
                     EFNIGHNIIARAIFVGLETAVREMRAAMSQSTEDSV"
     gene            complement(2565..2975)
                     /locus_tag="VFO72_03935"
     CDS             complement(2565..2975)
                     /locus_tag="VFO72_03935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET9478466.1"
                     /translation="MAEKIELEVVTPERRVLAESVDMVTVPGLGGELGILPGHTPLIS
                     QLQTGVLTYLQDGKSFSLHVSGGFVEVREDHVSVLAEVAERPEEIDAARAKLSRERLE
                     KQLNAWSGTEEDFEIARAKLERSVVRLQLASSRS"
     gene            complement(3060..4586)
                     /gene="atpD"
                     /locus_tag="VFO72_03940"
     CDS             complement(3060..4586)
                     /gene="atpD"
                     /locus_tag="VFO72_03940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9478467.1"
                     /translation="MATPNGNVTADGRPVGKVVQIIGPVLDVEFHGGYLPPIYQALRV
                     VSDGFDVPTPIDVIAEVQQHLGEGRVRTVSMTPTDGMVRGMQAIDTGGPITMPVGEGT
                     LGRVLNVIGEPVDTLGPVKHDKRYPIHRHAPTFEEQSTELQMFETGIKVIDLIQPFLR
                     GGKIGLFGGAGVGKTVIVMELINNIAKARAGFSVFAGVGERTREGNDLLREMVESQVI
                     QFGDEFNKNFHDTGKFDLTKVDMNTIGQSKVALIYGQMTEPPGARLRVALSGLTVAEY
                     FRDQGLDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPNLATEMGEMQERITSTKT
                     GSVTSIQAVYVPADDYTDPAPATTFAHLDAVTALSRQIVELGIYPAVDPLASSSRILD
                     PRVIGKEHYDTAQDVKRVLQRYKDLQDIIAILGLDELSDEDKLIVSRARKIQKFFSQP
                     FFVAAQFTGLEGKYVKLEDTVRGFRELVDGKHDDLPEQAFYMVGTIEEAVEKAKTIAQ
                     "
BASE COUNT         1048 a         1454 c         1452 g         1108 t
ORIGIN      
        1 gccgcatgcc ctgctcttcc aggcgcttca tcgcgtttgc cagctcttcg tcgctcgaac
       61 gctgaaaacc accggtgaga ccgatgtaac ccgtgcccgg ccgaatcatg taagcgttgc
      121 gaatcgtcag caacggcact gcctcgcgaa caatcgtgaa atactgcggg gcttcagaac
      181 cggcgcgttc cactttcaac ctcaccggtt cgcccagggc accgcgcaca ttcttcgaaa
      241 cttgttcgct gctccagtcg cgcgcgtctt tgccgtcgac ttcaagaatt cgatcgccgt
      301 acttcagtcc gagtttggcg gccggcgtac cttcgaccgc cgactgaacg tacacgccgt
      361 cgcgatgcga cacgatggtg acgccgattc cgtagaaacg agaatcctga tcttctttga
      421 gctttttaaa ctcgctgtac gggaagtacg cagagtgagg atcgagtgtg gaaagcatgc
      481 cctgaatggc ggcctgcgtt gctttttcgt gatcgatctc gccggagtag ttggaggtta
      541 tcgtcgcgac agcttcgttg tagtcccgtt cgatatcgtc ggcaacacgg tacgagtgct
      601 tggcgccgat agaagtcaga ctgctgaacc gccgagcgct gccgatagta ccgccaagtg
      661 ttgctgccac cagaattaca gcggtggcag tgatcgcaaa cttccaggca atagccttct
      721 ttcgatactt cttgaactgc tgtgcaactt ccaccctcga aatatagcac agggatttag
      781 tcattttcga ttgccaattg ccgattgccg attttccgat ttgctccctc gtgtcgattt
      841 gccctcggta tcgagcaatc aaacaattgg caattggcaa ttggcaatcg gcaatgaccg
      901 gctgtgttat atttcaaacg agcaccttca atgtcagcgt ctctctcgat cgttattcct
      961 gcgtataacg aggccgttcg tttgggaaac accctgccgg cggtcgtaaa ctacttgcgc
     1021 gaaagctggc ccgacgctga attaatcgtt gtcgacgatg gttcccgcga cgacaccgcg
     1081 gcgctcgcgc gtgctatctt caagaacaac gccggcctgc gtacttcggt catcagctac
     1141 aaatcaaatc tcggaaaggg ccgcgccgtc agactcgggc tgcttgcggc gcgtggcgac
     1201 gtcgcgctct tttcagacgc cgatctttcg acgccgatca ctgaagcacc gaaactgatc
     1261 gagccgattg tgagcggcca gtatgatgtg acgttcggtt cgcgcgcgtt ggatcgccgc
     1321 ctgatcggag tgcaccagcc atggcggcgc gaacagggcg gaagagtttt caatctggtc
     1381 gtgcggctgg cgactggatt gccgttctgg gacacacaat gcgggttcaa ggcgtttcgc
     1441 atgagtgtgt gccggccgct ggttgaggca gcaacggtgg accggtttgg ttttgatgtt
     1501 gaattgctgt atctggcttt tcgcgccgga ctgagattga aagaagtgcc ggtgcgctgg
     1561 gaccacaacg aaggcagtaa ggtgagcgtc atgagcgaca gctttcgcat gttgagtgag
     1621 gtgggactaa tccggcagca agcgcgccgc ggcgtttacg acagcgcgat ccgagcggtg
     1681 cacgagctta cgagtgctta gagctttgta ctttgtactt tgtactttgt gttagacagt
     1741 cgacagtggt agaacaaagt acaaagcgca aagtagaaag atcatgaccg cccgtctcaa
     1801 catcaacatc gatcacgtcg ctaccgttcg gcaggcgcgg cgcgctcccg agccgaactt
     1861 aatcgctgcc gcgatggcgt gcgaacaagc cggcgccgac ggcatcacgg tccacctgcg
     1921 cggcgaccgc cgtcacattc aggatctcga cattcggctg ctccgcgatt caatcatcac
     1981 ctacctcaac ctggaaatgg ccgcgactga cgagatgctc gatattgctc tgcaaacagg
     2041 acctgacgcc gtcacgctcg ttgccgaacg tcccaacgag atcaccaccg aaggtggact
     2101 cgatgtgcaa gcaaacgtcg cggtcgtaaa agctgccgtg gaccggctcc gtgaagcgag
     2161 catacttccg agtttgttta tcgatccgga tccggcgcag atcgaagtgg cgcatcagat
     2221 cggcgcgcaa caggtcgaac tgtgcaccgc cgcttacgca gaagcgacgc tgggcgcgcg
     2281 cggcattcac ggcgagggag cggcgcgagc cgcgcgtgaa ttgcagaggt tgaaagaggg
     2341 cgcggagctc gcgctgcgta agggattgaa cgtcgccgcg ggtcacggcc tgacttatcg
     2401 aaacgttggt gcgcttgcgc ggatcagtga gatcaccgag ttcaatatcg gccacaacat
     2461 catcgcgcgc gcaatttttg tcggactgga aacggccgtg agagaaatgc gtgccgctat
     2521 gagtcagtcc acagaagatt ctgtgtaatc tgtggatggt ttgcttagct tcgagatgag
     2581 gcgagttgca agcgcacgac ggatcgttcc agcttcgcac gcgctatttc gaagtcctct
     2641 tccgttccac tccacgcatt aagttgtttc tcgaggcgct cgcgcgacag tttcgcacgc
     2701 gccgcgtcga tctcttccgg ccgctccgct acttcggcga gcaccgaaac gtgatcttcg
     2761 cgtacttcga cgaacccgcc ggaaacgtgt aacgaaaaac tcttgccgtc ctgaaggtac
     2821 gtgagcacgc ccgtttgcag ctgcgaaatc aacggcgtgt gtccaggcaa aatacccagc
     2881 tcaccgccca aaccgggcac ggtgaccata tcaaccgatt ccgcgagcac acgccgttcc
     2941 ggcgtaacta cttcgagttc gatcttctcc gccatagcca ttattcgagt cggtgttcat
     3001 tcgcggcagt ccgtggccac agattcacac ggatggacac agaccgacca tcagtcgggt
     3061 tactgggcga tcgtcttcgc tttttcgact gcctcttcaa tggtgccgac catgtagaac
     3121 gcctgctcgg gaagatcgtc gtgcttgccg tcgacgagtt cgcggaaacc gcgcacagtg
     3181 tcttcaagtt tcacgtactt cccttcgagt cccgtaaact gagcggccac aaagaacggt
     3241 tgcgagaaga acttctgaat cttccgcgca cgcgacacga tcagcttgtc ttcatccgag
     3301 agctcgtcga gacccaggat agcgatgatg tcctgaaggt ctttgtaacg ctgcaacacg
     3361 cgcttcacat cctgagccgt gtcgtagtgt tccttaccga tcacgcgcgg gtcgagaatg
     3421 cgcgatgaag acgccagcgg atccaccgcg ggataaatgc ccaactcgac gatctgtcgc
     3481 gacaacgctg tgaccgcgtc gaggtgcgcg aacgtggtcg caggcgccgg atcggtgtag
     3541 tcgtcagcgg gcacgtacac ggcttgaatc gaagttaccg aaccggtctt cgttgacgtg
     3601 atgcgctcct gcatttcgcc catctcggtc gcgaggtttg gttggtaacc cacggccgaa
     3661 ggcatgcggc cgagcagtgc cgatacttcc gaacccgcct gcgtgaaccg gaagatgttg
     3721 tcgatgaaca gcagcacgtc gagaccctga tcgcggaagt actcggcgac cgtcaggccc
     3781 gagagcgcca cgcgcagacg cgcgccgggc ggctctgtca tctgtccgta aatgagcgcc
     3841 actttcgatt ggccgatggt gttcatgtcc actttcgtca aatcaaattt gccggtgtcg
     3901 tggaagttct tgttgaactc atcgccgaac tggataacct gcgactccac catttcgcgg
     3961 agcaggtcgt ttccttcgcg ggtgcgctcg ccgacgccgg cgaacaccga aaagcccgcg
     4021 cgcgctttcg cgatgttgtt gatcaactcc atcacgataa cggttttgcc cacgcccgcg
     4081 ccgccgaaga gtccgatctt gccgccgcgc aagaacggct gaatcaggtc gatgaccttg
     4141 atgcccgtct caaacatctg gagctcggtc gactgttcct cgaatgtcgg cgcatgacga
     4201 tgaatcggat agcgcttgtc gtgtttcacg ggaccgagtg tgtccacggg ttcgccgatg
     4261 acgttcagca cgcggccgag tgtgccttca ccgaccggca tcgtgatcgg gccgccggtg
     4321 tcgatcgctt gcatgccgcg caccatgccg tcggtgggcg tcattgaaac ggtgcggacg
     4381 cggccttcgc cgaggtgctg ctgcacttcc gcgatcacgt cgatcggagt gggcacatcg
     4441 aaaccgtcgg agacgacgcg cagtgcctga taaatcggcg gcaggtatcc tccgtgaaac
     4501 tcgacgtcga gaacggggcc gatgatctgc acgactttgc cgaccgggcg gccgtcggcg
     4561 gtcacgtttc cattaggcgt agccatactg tgtacaaact cctgttacgg gccaataaaa
     4621 cggtaggttt aagagccgca caagatacca cacccgatta gttcgagcaa aatcggccac
     4681 ggacaaacac ggatcttcac ggagctgatg aatacattaa gacgtccgca tacattaaga
     4741 cgtctgattg cattgagacg catctgaata cattgagacg tctgaatgcg ttgagacgca
     4801 tctgaataca ttgagacgtc tgaatgcgtt gagacgtccg tgtcatccgt gtttatcagt
     4861 ggcggaactt tgtagacggt taggtattag tgcaggacgg agtcagaggc taaacttccg
     4921 gatcgatcct gatcttcatg cgcggtagca cttgcaggtc ttcttcagtg agattgagat
     4981 tcgcggcttc gccttcgctg tcgtcgtcac cggcgtgagg ccgcaaccca atctcgagtc
     5041 gcagcgtcaa ctccatctcg ta
//
LOCUS       DASTEY010000219         5430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_565167, whole genome shotgun sequence.
ACCESSION   DASTEY010000219 DASTEY010000000
VERSION     DASTEY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565167"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFO72_03945"
     CDS             complement(<1..436)
                     /locus_tag="VFO72_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9478468.1"
                     /translation="MSQRRARLVLLIVVDQLRYDYLERFGDLFGPNGLRRLLRDGASW
                     SQANYDHTPTYTAPGHGTMMTGAHPSESGIIANEWIERASGKRISSVSDETVKLLGSG
                     DPAEGAASPRRLLSSTVGDELRLVTNGRSKVIGISVKDRSAIL"
     gene            complement(576..1799)
                     /locus_tag="VFO72_03950"
     CDS             complement(576..1799)
                     /locus_tag="VFO72_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478469.1"
                     /translation="MTLLMIIRVAFRALIRNKMRAALTMLGIIIGVSAVIAMVSIGQG
                     ASASVQAQIESIGTNLLFVSAGAQNVGGVRSGAGDSGTNTLTVEDLEAIRHEVPSVSM
                     VTPAVNARSQLVAGNMNWNTSVQGVSEEYPEIRKWNVQSGAFFTDADVRTAARVVVIG
                     QSIGDNLFPGMDPVGQTLRVANLPFRVVGVMARKGQDQAGRDQDDIAFAPYTTVQKKV
                     LGSPRVQIAYVSAISQDATYTAQSQITDLLRQRHKLTANEPNDFTVRNMTDIAEAANE
                     TSQTMTILLACIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARSSAVRSQFLI
                     ESIVLSLTGGAIGIILGIAFSLAIPAMLGWPTLVSLMAIAGSVLFSVAVGIFFGYYPA
                     RKAASLDPIEALRYE"
     gene            complement(1835..2665)
                     /locus_tag="VFO72_03955"
     CDS             complement(1835..2665)
                     /locus_tag="VFO72_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478470.1"
                     /translation="MSTTFSTQIPQIHPDASLLAQILEARQIIELDHIHKVYTMGDVE
                     VHALRGVSLTIREGEFVAIMGASGSGKSTMMNIVGCLDRPTRGTYILDGEDVSQLSKD
                     ERADIRCQKIGFVFQGFNLLSRTSALENVELPMLYLGVDAAARHQRAMDALAAVGLAG
                     REQNHPNQLSGGQQQRVAVARSLVNNPALILADEPTGNLDSRTSVEVMEIFQRLNRER
                     GMTLMLVTHEPDIAQYAQRVVVFKDGKIKSDQPVLDQRDAAEELKNLPAIEDDEDDEE
                     "
     gene            complement(2668..4350)
                     /locus_tag="VFO72_03960"
     CDS             complement(2668..4350)
                     /locus_tag="VFO72_03960"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9478471.1"
                     /translation="MATEIIKQKPLATGLGTGRSLAWLQQFWARVMAGKKKLFLGGGF
                     MAAVVFAGFYFWGTDSGTAEFLTARVDRGNLRNTVTATGTLQAVTTVQVGSQASGTIS
                     ALYADFNSIVKKGQVIAQLDPAVAKAQVEQARANLQQARASLAQARAAVTNSRAGITD
                     AQARSLAAKSTVQNNQAGVSAAEANVAVLKAQQDDAISLLKQQESLLKAGVIAQRDYD
                     VAVTAYKTAEARYSQAVAQLNQSKLSEQSSASAGIAQSQAAVEQSKAQLQQAQAQVEQ
                     NAAQVQQTEAALSLAEVNLSHTTITSPIDGVVVSRDVNVGQTVAASLSAPTLFTIAND
                     LTQMQVIANIDQADIGLVEQAKSVKFTVDAFPGKDFDGKIQEMRLNPVNVQNVVTYNV
                     VINVENPEQTLKPGMTANLTITIDERNNVLKVPNSALRFRPQNANNQSGNSGNGNATG
                     QGQGRRRQQGEGASSADGGRDSNFAPASAPVLPGQTRVVWVMGQDSKPQPRRIKVGLS
                     DGAATEVVEGNLNEGDMVITGQTVSDSGSATSTQQNRPPGFGNAPRVGRGRR"
     gene            4500..5021
                     /locus_tag="VFO72_03965"
     CDS             4500..5021
                     /locus_tag="VFO72_03965"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HET9478472.1"
                     /translation="MKKLGRFKMLAIATLSAIALAASIAIAQTATQDQGTGQGARMSQ
                     GRRGDHKGHGMRGMRAGGFFKQLNLTEDQKAKMKQIRESFAATNKPLREQLRAKRQEL
                     RQAREGGTFNEALATQKLTEIAPLQARLMAERAKLHQEMLSVLTAEQKAQLEQARAQF
                     KTRRGEMRKRHNQ"
     gene            5151..>5430
                     /locus_tag="VFO72_03970"
     CDS             5151..>5430
                     /locus_tag="VFO72_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9478473.1"
                     /translation="MDLDLARQAQDGDESAFAEIVRRYSPRVFSTASRFFRQRSLVEE
                     AAQEVFLKAFTQLGSYEGRGSMEGWLTRITTNTCLNMLRGAKRRPELTV"
BASE COUNT         1141 a         1590 c         1525 g         1174 t
ORIGIN      
        1 gcagaatcgc agagcgatct ttaacggaga ttccgatcac cttcgatcgg ccgtttgtga
       61 cgagtcgtag ttcgtcgccg acagtagaac tcaagagtct gcgcggacta gctgcgccct
      121 cggcgggatc gccgctgccc agcagtttta cagtttcgtc cgaaacactg ctgatgcgct
      181 ttccgctagc ccgttcgatc cattcgttag cgataattcc cgattcagac ggatgcgctc
      241 ccgtcatcat cgtgccatga ccgggcgcgg tgtaagttgg cgtgtgatcg tagttcgcct
      301 gactccaaga ggcgccgtcg cgcaacaacc gccgcaagcc atttggccca aacagatcgc
      361 caaaccgttc gaggtaatcg taacgcaact ggtccaccac tatcagcaac accagccggg
      421 cgcgtctctg cgacacctgc gtaccacgcc gttgcgcgct cgcaagcggg agagttgaaa
      481 agattgtgat tagaagcaga caaagagcca cggcggagga tcgccggcgc ataggcgact
      541 gattcatgcg ccggatgtta acctatccgt ggcccttact cgtagcgcaa cgcttcgatt
      601 ggatcgagcg aggcggcttt gcgtgcgggg taataaccga aaaaaattcc caccgcgact
      661 gaaaagagaa ctgagccggc aatcgccatc agggagacga gcgtgggcca accgagcatt
      721 gctggtatcg cgagcgagaa cgcgatgccg agaatgatgc caatcgctcc accagtcaga
      781 ctcagcacga tcgattcaat caaaaactgg ctgcgcactg ccgaggaccg cgcgccgatc
      841 gccatgcgaa tgccgatctc acgcgtgcgt tccgtcacgg acaccagcat gatgttcata
      901 atgccgatgc cgccgacgag cagcgaaacg cccgcgatgc acgccagcag gatcgtcatc
      961 gtttggctgg tttcgttcgc ggcttccgca atgtcggtca tgttacgaac cgtaaagtcg
     1021 ttcggctcgt tggccgttaa cttgtgacgc tggcgcagca agtccgtaat ctgcgactgt
     1081 gccgtgtagg tcgcgtcctg agaaatagcc gacacatacg cgatttgaac gcgcggacta
     1141 cccagcactt tcttctgcac ggtcgtgtac ggcgcgaagg cgatgtcgtc ctgatcgcgg
     1201 cccgcctggt cctgcccttt acgcgccatc acgccgacaa cacgaaacgg caggttcgcg
     1261 acgcgcaggg tttgtccaac cggatccatt ccggggaaga ggttgtcgcc gatgctttgt
     1321 ccgatgacga ccacgcgcgc ggctgtgcgc acatctgcgt cagtaaagaa cgcgccgctt
     1381 tgcacattcc acttgcgaat ctcgggatat tcttcgctga ccccctgaac cgacgtgttc
     1441 cagttcatgt tgcctgcgac caactgcgac cgggcgttca cagcgggcgt caccatcgag
     1501 acgctgggaa cttcatgcct gatcgcttcg aggtcctcaa ctgtcaacgt attcgtgccg
     1561 ctgtcacccg cgccactgcg cacgccgccg acgttttgtg cacccgcact gacgaagagc
     1621 aggttcgtac cgatgctttc gatctgcgcc tggacagacg ccgacgcgcc ctgaccgatg
     1681 ctgaccatcg caatcacggc agacacgccg atgatgatgc caagcatcgt caaagccgcg
     1741 cgcatcttgt ttctgattag cgcgcgaaaa gcaacacgga tgatcatcaa aagggtcatt
     1801 gtagatttcc gattttcaat tgccgatttc cgatttattc ctcgtcgtcc tcgtcgtcct
     1861 caatcgccgg caggttcttt agttcttcgg ccgcgtcacg ttgatcgagt acgggctggt
     1921 cgcttttgat ctttccgtct ttgaacacca ccacacgctg cgcgtactgc gcaatgtccg
     1981 gttcgtgcgt gacgagcatg agcgtcatgc cgcgttcgcg attcaaccgt tgaaaaatct
     2041 ccatcacttc aaccgacgtg cgcgagtcga ggttaccggt cggctcgtcc gccagaatca
     2101 acgccggatt gtttaccagc gaacgcgcaa ccgcgactcg ctgttgttgt ccaccggaga
     2161 gttgattcgg atggttctgt tcccggccgg cgaggccgac agcggccagc gcgtccatgg
     2221 cgcgctggtg ccgtgctgca gcgtcgacgc cgagatagag catcggcagt tcgacgttct
     2281 ccaacgcgga agtacgcgaa aggagattga aaccctgaaa cacaaagccg atcttctggc
     2341 agcgaatgtc ggcgcgctcg tctttcgaca actgcgaaac atcttcgccg tccaggatgt
     2401 aggtgccgcg cgtggggcgg tcgagacaac cgacgatatt catcatcgtt gacttgcccg
     2461 aacctgacgc gcccatgatc gccacgaact cgccttcgcg aatcgtcagc gagacgccgc
     2521 gcagcgcgtg aacctcaacg tcgcccattg tgtagacctt atgaatgtgg tccaactcga
     2581 taatctgacg cgcctcgagg atttgcgcca gcaaagacgc gtccggatga atctgtggta
     2641 tctgtgttga aaacgtggtt gacataatta cctccgacca cgaccgacac gaggagcgtt
     2701 accgaatccc ggcggtctgt tctgctgtgt cgatgtcgcc gaaccggagt cactcactgt
     2761 ttggccagtg atgaccatgt cgccttcgtt caagttgccc tcgacaactt cagttgcggc
     2821 gccgtcgctc aggccgactt tgattcgtcg cggttgcggc ttgctatcct ggcccatcac
     2881 ccagaccacg cgcgtctgtc ccggcagcac cggcgctgaa gccggagcga agttactgtc
     2941 acgaccacca tctgcactcg acgcgccctc accttgctga cgacggcgtc cctggccctg
     3001 accggtcgcg tttccattgc cgctgttgcc ggactgattg ttcgcgttct gcggcctgaa
     3061 tctcagcgcg gagttcggca ccttcaacac gttgttgcgt tcgtcaatcg tgatcgtcag
     3121 gttcgccgtc atgcccggtt tcagcgtctg ctccggattc tctacgttaa tgacgacgtt
     3181 atacgtgacg acgttctgca cgttgaccgg attcagtcgc atctcctgga tctttccgtc
     3241 gaaatccttt ccgggaaagg cgtcaaccgt gaacttcacc gatttcgcct gttcaaccag
     3301 accgatgtct gcctgatcga tgttggcgat gacctgcatc tgcgtcaggt cgttcgcaat
     3361 tgtgaaaagc gtcggcgccg acaagctcgc cgcgacagtc tggcctacgt tcacgtcacg
     3421 cgaaacgaca acgccgtcga tcggcgaggt gatcgtcgtg tgactcaaat tcacttcggc
     3481 caaactaagc gcagcttcgg tctgctgaac ttgcgctgca ttctgttcga cctgggcttg
     3541 ggcctgttgc aactgcgcct tcgattgttc gaccgcggcc tgcgattgag caatgcccgc
     3601 acttgctgac gactgttcgc tcagcttcga ctgattcagt tgcgccaccg cctggctata
     3661 acgagcttca gcagtcttgt aagcggtcac tgcgacgtcg taatcgcgtt gcgcgatcac
     3721 acccgcttta agcagtgact cctgctgttt caaaagactg atcgcgtcgt cctgctgagc
     3781 tttcaatacg gcgacattgg cttccgccgc ggagacgccc gcctgattgt tctggactgt
     3841 agatttcgct gcgagactgc gcgcctgtgc atcagtgata ccggcacgag aattcgttac
     3901 ggcagcccgc gcttgcgcga gactcgcgcg cgcctgttgc agattcgcgc gggcctgttc
     3961 gacctgcgcc tttgcaactg ccggatcgag ctgcgcgatg acctgtcctt ttttaactat
     4021 cgaattgaag tcggcgtaaa gcgccgagat cgtgcccgac gcctggctgc ccacctgaac
     4081 agtggttacg gcctgcaaag tcccggtggc ggtgacggtg ttacgcaaat tgccacgatc
     4141 gacacgtgcg gttagaaatt cagcagtgcc ggaatcggta ccccaaaaat aaaatccggc
     4201 gaacacgacg gctgccatga agccgccgcc aagaaacaac ttcttcttgc cggccatcac
     4261 tcgcgcccaa aattgttgaa gccacgcgag gctgcggccc gtgccaagtc cagtcgcgag
     4321 cggtttttgt ttaatgattt cagtcgccat agttatcctc aaccaacaag acaccggtag
     4381 cagcaggtcg gttacacagg atctcaggaa tttctgtaac caatcctgga cagggcgtgt
     4441 cttaccgatt gggacactcc cctggaatga acaaaggtaa ttaggagtaa ggacatagaa
     4501 tgaagaaact tggacgtttt aagatgctgg caatagctac tctctcggca attgctttgg
     4561 ctgcttcgat cgccatcgct cagacagcta cccaggatca gggcactggc cagggtgcgc
     4621 ggatgtcgca gggccgtcga ggtgatcaca aaggacatgg catgagagga atgcgcgccg
     4681 gcggattctt caaacagctt aatttgactg aggaccagaa agcgaagatg aagcagatcc
     4741 gcgaaagctt tgccgcaacc aataaacccc tgcgcgaaca gcttcgggcc aagcgacagg
     4801 agttgcgtca ggcgagggaa ggcggtacgt tcaacgaagc actggctact cagaaactga
     4861 cggaaatagc tccgctccaa gcgaggttga tggccgaacg agcgaagctt caccaggaaa
     4921 tgctttccgt tttgacggct gagcagaaag cccagttgga acaggcacgc gcgcagttca
     4981 aaacccgtcg tggcgaaatg cgcaaacgcc ataatcagta agcgtctccc cccgcttctt
     5041 tggagtgcgc cgtcttgtcg gcgctttgat taaggtagtg acagatcaag cgcactccaa
     5101 ggaggtgtta gagatgagga ccgcaactga cgagtgggct cgaacaggcc ttggacctcg
     5161 acctggcgcg acaagcgcag gacggagatg agtctgcttt tgctgaaata gtcaggcgct
     5221 atagcccgcg agtctttagc acagcaagcc ggttctttcg tcagcgcagt ttggttgaag
     5281 aagccgcgca ggaagttttt ttgaaagcgt ttacgcagct cggaagttat gaaggccgcg
     5341 gttcgatgga gggttggtta acaaggatta cgaccaacac ctgtttgaat atgttgagag
     5401 gggcgaaacg gcggccggag ctgactgttt
//
LOCUS       DASTEY010000220         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_631275, whole genome shotgun sequence.
ACCESSION   DASTEY010000220 DASTEY010000000
VERSION     DASTEY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631275"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFO72_03975"
     CDS             complement(<1..531)
                     /locus_tag="VFO72_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HET9478474.1"
                     /translation="MRWSRFFIPTLREDPADAEVASHKLLLRAGVIRQLSAGIYSLLP
                     LGQRVALKVMQILREEMNAIGGQEFYLPALHPAEIWQESGRWYDIGEEMFRLKDRKGA
                     DMCLGMTHEEIFTSIARHEIRSYKQLPQVWYQIQLKFRDEARPKSGLIRVRTFIMKDA
                     YTFDVDRAGLDRAFLDQ"
     gene            676..1119
                     /gene="rpiB"
                     /locus_tag="VFO72_03980"
     CDS             676..1119
                     /gene="rpiB"
                     /locus_tag="VFO72_03980"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9478475.1"
                     /translation="MRIALASDHAGYAEKERLKTVLTELGVDFDDLGPASDDSVDYPD
                     YARLVAEQVAQGQVEQGLLVCGSGTGMAITANKVRGVRAAVAWSEETARLARQHNDAN
                     VLALGARTTPPADIPKIVRAWFSTDFEGGRHAARVAKICEVERSY"
     gene            1119..2402
                     /gene="glyA"
                     /locus_tag="VFO72_03985"
     CDS             1119..2402
                     /gene="glyA"
                     /locus_tag="VFO72_03985"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9478476.1"
                     /translation="MTDSRNSPLAEVDPVIAGAIDNEVARQANGLELIASENFVSEAV
                     LEAAGSVFTNKYAEGYPGKRYYGGCEWTDVVEQTAIDRAKQLFGADHANVQPHSGAQA
                     NMAVYLAAIQYGDQILGMNLSHGGHLTHGHPMNFSGMSYKVADYGVSRETEQIDYDEL
                     QRIAEEHRPKLLVCGASAYPRTINFERIGEIARSVGARVFADIAHIAGPIAADLHPSP
                     VPHADYVTTTTHKTLRGPRGGLILCKEEHAKEIDRKLFPGVQGGPLVHIIAAKAVALG
                     EALRDDFKEYQSQVLQNAKALASELQEQGLRLVSGGTDNHLMLVDVWMDGKGITGKAA
                     EKALEAALITVNKNTIPFDQNKPFVASGLRIGTPAVTTRGMKEPEMREIGRLIAEVIH
                     APESEEIRTKVRRDVAALTARFPLYSKRLKSVSSV"
     gene            2414..>3049
                     /locus_tag="VFO72_03990"
     CDS             2414..>3049
                     /locus_tag="VFO72_03990"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9478477.1"
                     /translation="MLEVFGPEGLIAKAHPEYEYRPGQVEMARAVMCAFEEQRHLIVE
                     AGTGTGKTLAYLVPAVAAALGGRGRVIISTGTKNLQEQLMEKDIPFLQNVLPKPFTAT
                     YMKGRNNYLCLNRLTRAQSAPVLEGLEDVDYFEEVCQWSRQSETGDRAELANLPESLS
                     FWRHIDARSESCLGQKCPDFDPCFITRMRDRAQEADVIVVNHHLFFADLSLR"
BASE COUNT          672 a          830 c          904 g          643 t
ORIGIN      
        1 ttgatcgaga aacgccctgt cgagtccggc tcggtcaacg tcaaacgtgt aagcgtcctt
       61 catgatgaaa gtgcgcacgc gtatcaagcc cgactttggc cgcgcttcat cgcgaaactt
      121 gagctggatc tgataccaaa cctgcggcag ttgtttgtaa gaacgaattt catggcgcgc
      181 gatgctggta aagatctctt cgtgcgtcat gcccaggcac atgtccgcgc ctttgcgatc
      241 cttaaggcga aacatctctt cgccgatgtc gtaccagcgg cctgattcct gccatatttc
      301 tgcgggatgg agtgcgggca gataaaactc ctggccgccg atggcgttca tctcttcgcg
      361 gagaatctgc atcaccttga gggcgacacg ttggcccaac ggcaacaggg aatagatgcc
      421 ggcggaaagt tggcgaataa cacctgcgcg aagcaggagt ttgtgggaag cgacctcggc
      481 gtctgccggg tcttcacgga gagtagggat aaagaatctt gaccagcgca tagaccgtgc
      541 attctcgctt agcggctgcg gctaaggcaa atgagccaca gacgaacacg gattttagac
      601 agcctttgcc gcgcggattg gagtgagtta ccatcaccgt ctttagtcac actcctctaa
      661 gtaggttcta ttaacatgag aattgctctg gccagcgatc acgctggtta cgctgagaag
      721 gagcggttga agacggtgct gacggagctg ggtgtcgact ttgacgacct cggccctgcg
      781 tcagacgact cggttgacta tcccgattac gctcgcctgg tcgcggagca ggttgcgcaa
      841 ggtcaggtcg aacagggact gctcgtttgc ggctccggca ccggcatggc catcaccgcc
      901 aacaaagttc gcggcgtgcg ggctgcggtc gcgtggtctg aagagacggc gcggcttgcg
      961 cgccaacaca acgacgccaa cgtactcgcg ctcggagcac gcacaacacc gcccgctgat
     1021 attccgaaga tcgtgcgcgc ctggttctct accgattttg aaggtggccg tcacgccgct
     1081 cgcgtggcaa agatttgcga agtggagcga agttactaat gactgattct cgaaactcac
     1141 cgcttgccga agttgatcca gtgatcgctg gcgcgatcga taacgaagtc gcgcgtcagg
     1201 cgaacggatt ggaactgatc gcgtcggaaa actttgtcag cgaagcggtg cttgaagctg
     1261 ccggttcggt gttcacaaac aagtacgccg agggttatcc aggcaagagg tattacggcg
     1321 gttgcgagtg gacggacgtc gtcgaacaga cagcgattga ccgcgcgaaa caactgtttg
     1381 gcgcggacca tgccaatgtg cagccgcaca gtggcgcaca ggcgaacatg gcagtctatc
     1441 ttgccgctat tcagtacggc gatcagattc tcggaatgaa tctgtcgcat ggcgggcacc
     1501 tgacgcatgg tcatccgatg aacttttcgg gaatgagtta caaggtcgct gattacggtg
     1561 tttcgcgaga aacggagcag atcgattacg acgagttaca gcggattgcg gaagagcatc
     1621 gcccgaaatt gcttgtgtgc ggcgcatcgg cctatccgcg gacgatcaat ttcgagcgca
     1681 tcggtgagat tgcgcgaagt gtcggtgcgc gtgtgtttgc ggatatcgcg catatcgcgg
     1741 gcccaatcgc cgctgatttg catccttcac cagtgccgca cgccgattac gttaccacga
     1801 caacgcataa aactttgcgc gggccacgcg gcggcttgat tctatgcaag gaagaacatg
     1861 caaaagagat cgatcgcaaa ctgtttccag gcgtgcaggg tgggccactg gtgcacatca
     1921 tcgcggcgaa ggcggtggct ttgggtgaag ccttgcgcga cgacttcaaa gaatatcaaa
     1981 gccaggtttt gcagaacgct aaagcgctcg cgagtgaact tcaggaacag ggactgcgtc
     2041 tcgtgtccgg tgggactgac aatcacttga tgctggtcga cgtttggatg gacggcaaag
     2101 gaatcaccgg caaagccgcc gaaaaagcac tcgaagccgc attgatcacc gtcaacaaaa
     2161 acacgatacc gttcgatcaa aacaaaccgt ttgtcgcgtc cggcctgcgc atcggcacgc
     2221 cggcagttac cacgcgcggt atgaaagaac cggagatgcg tgagatcggc cgtctcatcg
     2281 cggaagtgat ccacgccccc gaatctgaag agatccggac caaagttcgc cgcgacgtcg
     2341 ccgccctgac cgcgcgtttt ccgttgtatt cgaaacggct gaaatccgtg tcttccgtgt
     2401 aaatctgtgg tctatgcttg aggtttttgg tcctgagggg ttaatcgcta aggcgcatcc
     2461 tgagtatgag taccggccgg gccaggtgga gatggcgcgt gccgtgatgt gtgcgtttga
     2521 ggagcagcgc cacctcatcg tcgaagcggg caccggaacg ggcaagacgc tcgcctatct
     2581 tgtgcctgcg gttgccgcgg ctttgggtgg ccgtgggcgg gtgattatct caacgggcac
     2641 caagaacctg caggaacagt tgatggagaa agacattccc tttcttcaga acgttttgcc
     2701 aaagcccttc acggccacgt acatgaaggg ccgcaacaat tatctttgtc tcaatcgact
     2761 cacgcgcgcg caatcagcgc ccgtgttgga aggactcgaa gacgtcgatt acttcgaaga
     2821 ggtctgtcag tggtccaggc aaagcgaaac gggcgatcgc gccgaactcg ccaaccttcc
     2881 cgagtcactt tcattctggc gccacatcga cgcgcgctca gagtcgtgtc ttggacagaa
     2941 gtgccccgac ttcgatccgt gcttcatcac ccgcatgcgc gatcgcgctc aggaagctga
     3001 cgtcattgta gtcaaccatc acctcttctt cgccgatctc agtttgcgc
//
LOCUS       DASTEY010000221         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_449760, whole genome shotgun sequence.
ACCESSION   DASTEY010000221 DASTEY010000000
VERSION     DASTEY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449760"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="dnaG"
                     /locus_tag="VFO72_03995"
     CDS             complement(<1..737)
                     /gene="dnaG"
                     /locus_tag="VFO72_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET9478478.1"
                     /translation="MRYPQTFIDDLKRQADIVRVIQDYVQLKKKGANWMACCPFHKEK
                     TPSFSVSPTKEIFYCFGCHKGGSVFNFVMDIERVGFPEAIRIVADKVGMPLPKMVDDS
                     RFEARRQESDDVIQLNSWAMDWWEQQLESSKEARVAREYLVQRELSEDTQKTFRLGYA
                     PDSWDALSSYLRQKGATQQQIERSGLVVKKEEGGSYDRFRCRLMFPVLDVQGRPIAFG
                     GRTLKGEDAKYVNSPETSAYVKGRNLFG"
     gene            complement(770..>2237)
                     /locus_tag="VFO72_04000"
     CDS             complement(770..>2237)
                     /locus_tag="VFO72_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9478479.1"
                     /translation="AMIISGANAGGKTVVLKTTGLLALMALSGISVPAKSASFPFYAT
                     VLADIGDHQSIAANLSTFTSHVANIARMIELCEAPALVLLDEVGTGTDPEEGSALGVA
                     VVDHFRRVCDAHVIATTHYSGLKMYAGNEAGVINASVEFDEKTLRPTYRLIVGVAGAS
                     SGLEIARRFGVPRTIVDAAVGSVKDSSLQATEYLRRIKREAEEAEALRLALEEERAAV
                     AEKFASLDKQAAKRERERQTAFEQVAQRAVTDLEKRARELVGRIEDRTERIRAEREAQ
                     RQVAEMKRAAQSATKAAGQTKSIADSSAKFVSAGTAEHAGAEERVEYVTALPREIVAG
                     DKVKLLSFGSIGVVDQIKDGYADVRVKALRFREKLENLELMEQLPSAKPQHGKLEKLR
                     RSTGTEVHLATVDQKVQSELNVIGQTTDEAVDAVDKFLDEASLASLSQVRIVHGHGTG
                     ALRRAIAGLLGDHPHVARFLPAPPDQGGAGATLVELRQ"
BASE COUNT          439 a          680 c          578 g          540 t
ORIGIN      
        1 ccgaaaagat ttcgaccttt cacatacgct gatgtctcag gtgagttgac gtacttcgcg
       61 tcttcgcctt tcaacgtacg tccaccgaaa gcgatgggcc ggccttgcac gtccagcact
      121 gggaacatca gccggcaacg aaagcgatcg tatgatccgc cctcttcttt tttgacgacc
      181 agaccactgc gttcgatctg ttgctgtgtt gcgccttttt ggcgcagata cgaagagagc
      241 gcgtcccagc tatcgggcgc ataaccgaga cgaaacgtct tctgcgtgtc ttcactgagt
      301 tctcgctgca cgagatactc gcgagccacg cgcgcttctt tgctcgactc gagttgctgt
      361 tcccaccaat ccatcgccca cgaattgagc tgaataacgt cgtctgattc ctgccggcgc
      421 gcttcgaagc ggctgtcgtc caccattttc ggcagcggca ttccgacttt atcggccaca
      481 atgcggatcg cttccgggaa accgacgcgc tcgatatcca tcacaaaatt gaagacgctt
      541 ccgcccttgt ggcagccaaa acagtagaag atctcttttg tgggactaac ggagaacgac
      601 ggcgtctttt ctttgtgaaa cgggcagcac gccatccaat ttgcgccctt tttcttgagc
      661 tgcacgtagt cttgaatgac gcgaactatg tctgcctggc gcttcaggtc gtcgatgaat
      721 gtttgggggt aacgcattcg atccgccttg tccgtgtaat ctgtggcggt tattgtctca
      781 actcaaccaa cgttgcacca gcaccgcctt gatcgggagg ggcgggcagg aaacgagcga
      841 cgtgaggatg gtctcccaac aaaccggcaa tggcgcgccg caacgcgccg gtgccgtgac
      901 cgtgaacaat ccgcacctga ctcagactcg ccaacgatgc ttcgtccaaa aacttatcaa
      961 cggcatcgac cgcttcatca gtcgtctggc cgataacatt caattctgac tggaccttct
     1021 ggtccacagt ggcgaggtga acttcagtac cggttgagcg tcgtagtttt tcgagtttgc
     1081 cgtgctgcgg ttttgcgctg ggcaattgtt ccatcagttc gagattttca agcttttctc
     1141 gaaagcgtaa cgccttcacg cgcacgtccg cataaccgtc tttgatctga tctaccacac
     1201 cgatcgaacc gaacgagaga agtttaacct tgtcgccggc cacaatctcg cgcggcaagg
     1261 cggtgacata ctcgactctt tcttcagcgc cggcatgttc cgcagttccg gctgacacga
     1321 attttgcgga tgaatcagct atcgattttg tttgacccgc tgctttcgtc gcgctttgcg
     1381 cggcgcgctt catctctgct acctggcgct gcgcttcacg ctccgcacga attctctcag
     1441 tccgatcttc gattcgtccg accagttcac gcgctcgctt ttcgagatcc gtcaccgctc
     1501 gctgcgcgac ttgctcgaaa gctgtctgac gttcgcgttc gcgcttagcg gcttgtttat
     1561 caagcgaggc gaatttctct gcaaccgcgg cgcgctcttc ttcgagcgcg agtcgcaaag
     1621 cctcggcttc ttcagcctca cgcttgattc ggcgcagata ctccgttgcc tgtaacgaag
     1681 aatccttcac cgaacccact gcagcgtcca ctatcgtccg cggaacaccg aaccttcgcg
     1741 caatctccag cccggaggac gctccggcca ctccgacgat gagtcgatac gtcggtcgca
     1801 gtgtcttttc gtcaaactcg acactcgcgt ttatcacgcc cgcctcgttg cctgcgtaca
     1861 ttttgagtcc gctgtagtgt gtggtcgcga tgacgtgtgc atcgcacact cggcgaaagt
     1921 ggtccaccac cgcaacgccc agcgccgatc cctcttccgg atcggttccg gttccaactt
     1981 catcgagcag caccagcgcg ggtgcttcgc agagttcgat catgcgcgcg atgttggcga
     2041 cgtgtgacgt aaacgtagaa agatttgccg cgatcgattg gtgatcgccg atatcggcca
     2101 gcactgttgc gtagaaggga aaactcgccg acttcgctgg cacggaaatg cccgagagcg
     2161 ccataagtgc caacagcccg gttgttttca acacgactgt tttaccaccg gcattcgcgc
     2221 cggagatgat catagcg
//
LOCUS       DASTEY010000222         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_103634, whole genome shotgun sequence.
ACCESSION   DASTEY010000222 DASTEY010000000
VERSION     DASTEY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103634"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFO72_04005"
     CDS             <1..974
                     /locus_tag="VFO72_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9478480.1"
                     /translation="QERVQTLPGVSSAGTVDILPLTGGNTTRFYIDGDPVPPPGKEIE
                     ANTRVISDTYFQTLGVPLLAGRTFDQRDAPDKPSVIIIGKTIADRLFPGRDPVGKKIR
                     YASIQVDPVEIVGVVGDVKITGLDEAIRPVLYYPYRQNAGPFANLVVRAHSDPRALTA
                     SIRSEIRNLEPDAAILNVNTMDQMIAQTPASFMRRFPALLISIFAIVALLLASIGIYG
                     VVSYSVSQQTHYIGVRMALGASPADILKMVLKQGLVLALAGVAIGVVAALGLMRLLRT
                     LLFEVSTTDVATFTVAAGGLFVVALVACYLPARRATKVDPLVALRYE"
     gene            988..2610
                     /locus_tag="VFO72_04010"
     CDS             988..2610
                     /locus_tag="VFO72_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478481.1"
                     /translation="MITDLRYALRMLAKNLSFTLVAVLTIALGVGANTALFSVVDAVL
                     LKKLPVKDPDQLVLFNASWDPEKFGPGGFNGSNPYDPDLGLTVGTSFPVETLRRLHQE
                     HSVLSDVFAFSPMALNFNASGEAEVVSGQVVSGNYYTALGVTAILGRTITDVDDNLGV
                     TPVAVLSHRFWSSRFGSDPSVVGKQVNINNVAFTIAGVTPAGFSGTGNVGSSPDVSIP
                     LAWEPQVAGEQTNMRDAGIWWLRLMGRLQPGTTLEQAHATLAGPFQQSVVEHRAMRQA
                     RSKTTLGTINPKDLPRLGVDSGSQGEMDARNGFAVPLMLRFGPGNPGEIEIVGLARDA
                     KYRSQRAEIEPTVFQPWQQSLSTMRNATFEVRTAGEPASYIAGIRQAMREVDSNLPLS
                     NIRTQIQQANQTLAMERVFAKLLTLFGLIAQQLAAIGLYGVMAFAVSQRTHEIGIRMA
                     LGANRGNVLAMILRQGMSLTLIGVAAGLAAAYVLMNYLQSLTDLLFGVEATDVWTFAA
                     IGALLVVVALVACLVPARRATKVDPLIALRYE"
     gene            2623..>2897
                     /locus_tag="VFO72_04015"
     CDS             2623..>2897
                     /locus_tag="VFO72_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478482.1"
                     /translation="MINDIRYALRMLAKNPGFAAVVVFTLALGIGANAAIFSLMDRVL
                     LQSLPVANPEQLAVLSSYSKGEPQIDGSFSYPMYQGLRDRNNVFAGV"
BASE COUNT          574 a          798 c          864 g          661 t
ORIGIN      
        1 tccaggaacg cgtgcaaaca ttgccgggcg tttcgagtgc gggcacggtg gacatactgc
       61 cgctgaccgg cggcaacacg acgcgctttt acattgacgg cgatccggtg ccaccgccgg
      121 gcaaagagat cgaggcgaac acccgagtca ttagtgacac ttactttcaa actctcggcg
      181 tgccgttact cgcgggccgc acgtttgacc agcgcgacgc cccggataaa ccaagcgtaa
      241 tcatcatcgg caagacgatc gccgatcgac tctttcccgg tcgcgatccc gtcggcaaga
      301 agatccggta cgcatccatt caagtcgatc ccgttgagat cgtgggcgtt gtcggagacg
      361 tgaagatcac tggactcgat gaagcgatca ggcctgtgct ctactatccg taccggcaaa
      421 acgcaggtcc gttcgcaaac ctcgtcgttc gagctcatag cgacccaagg gcactcactg
      481 cttcgatccg cagtgaaatc cgcaatctcg aacccgacgc cgcgattctc aacgtcaaca
      541 cgatggacca gatgatcgct cagactccgg cgtcgttcat gcgccggttc ccggcgttgt
      601 tgatcagcat cttcgcgatt gttgctttgt tgctggcgtc gatcgggatc tacggcgtcg
      661 tctcttattc cgtcagccaa cagactcatt acatcggcgt gcgcatggcg ctcggcgcaa
      721 gtccggccga cattctcaag atggtgttga agcagggact ggtgttggca cttgcgggcg
      781 tggcgattgg agtggtggcc gcgctcgggc tgatgcgtct gttgcgcacg ttgttgtttg
      841 aagtgagtac gacggacgta gccacattca cggtggccgc tggtgggttg ttcgtcgttg
      901 ccctggttgc ttgttatctg ccggcgcgtc gcgccacgaa ggtggatccg ctggtggctc
      961 ttaggtacga atagaggatt tacacaaatg attacggatc ttcgttatgc gcttcgcatg
     1021 ttggcgaaaa acctcagctt cactctggtc gcggtgttaa cgattgcgct tggcgtcggc
     1081 gccaacaccg cgctcttcag tgttgtcgat gcggtcctgc tgaagaaact gcccgtgaag
     1141 gatccggatc agcttgtctt attcaacgcg agctgggacc cggagaagtt tggtccaggc
     1201 ggattcaacg gcagcaatcc atatgatccc gacctcggtt tgactgtggg aacttcgttt
     1261 ccagtggaaa cgctcaggcg ccttcatcag gagcatagtg tgctttccga tgtcttcgcc
     1321 ttttctccca tggcgctgaa cttcaatgcc agtggagagg cggaagttgt gagcggccag
     1381 gttgtttccg gcaattacta cacagcgctt ggtgttacag cgatcctcgg ccgaacgatc
     1441 accgacgttg acgacaacct gggcgtcact ccagtcgctg ttctcagtca tcgtttctgg
     1501 tccagccgtt tcggcagcga tccgtccgta gtcggtaaac aggtcaacat caataacgta
     1561 gcgttcacca tcgcgggagt aacgccggcc ggcttttcag gaacgggcaa cgttggttcc
     1621 tcgccggacg tttccattcc cctcgcatgg gagccgcagg ttgccggcga gcagaccaac
     1681 atgcgtgacg ccggtatttg gtggctgcga ttgatgggac gattgcagcc cggcacaacc
     1741 ctcgaacagg cgcacgcgac tctcgctggt ccattccaac aatcagtcgt cgagcatcgc
     1801 gccatgcgac aggcgcgatc gaagaccacg ctcggcacta taaatccaaa agacctgcca
     1861 cgactgggag ttgattccgg cagccagggt gagatggacg cgcgcaatgg tttcgctgtt
     1921 cctttaatgc tgcgttttgg gccgggcaat ccgggcgaga tcgagatcgt gggcctcgcg
     1981 cgagatgcga agtacaggag ccagcgcgct gaaatcgaac ctaccgtttt tcagccgtgg
     2041 cagcagtcgc tgtcaacgat gcgcaacgcg acgtttgaag tgcgcaccgc gggcgaaccc
     2101 gcttcataca tcgcgggcat acgccaggcg atgcgcgaag tcgacagcaa cttgccgctc
     2161 agcaatatca gaactcaaat tcaacaggcg aaccaaacac tcgcgatgga gagggtgttc
     2221 gcgaagctgt tgacgctttt tggattgatc gcacagcagc tcgccgctat tggtctctat
     2281 ggagtgatgg cgtttgccgt ctcgcaacgc acacatgaga tcggtattcg aatggcgctc
     2341 ggcgcgaacc gaggaaacgt gctggcgatg attctgcgtc agggaatgag tttgacgctg
     2401 atcggtgtcg cagccgggct agctgcggct tacgtgttga tgaactatct ccagagtctg
     2461 actgatttgt tgtttggtgt tgaggcaaca gatgtgtgga cgtttgccgc cataggcgcg
     2521 ctgctggttg tcgttgcgct ggttgcgtgt ctggtgccgg ctcgtcgcgc caccaaagtc
     2581 gatcccttga tagcgctgcg ttacgaataa aaaggaacag agatgatcaa tgacattcgt
     2641 tatgccttgc gcatgttggc gaagaacccc ggcttcgctg ctgtagtagt gttcacactc
     2701 gcgctgggca ttggcgcaaa cgcggccatc ttctcgctca tggatcgagt gttgttgcaa
     2761 agcctgccgg tggcgaatcc ggaacagctc gcggtgttgt cttcttactc gaagggtgag
     2821 ccacagatcg acggttcgtt ctcgtatccg atgtaccagg gtctgcgcga cagaaacaat
     2881 gtgttcgcgg gagtgat
//
LOCUS       DASTEY010000223         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_202556, whole genome shotgun sequence.
ACCESSION   DASTEY010000223 DASTEY010000000
VERSION     DASTEY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202556"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="hemA"
                     /locus_tag="VFO72_04020"
     CDS             complement(<1..920)
                     /gene="hemA"
                     /locus_tag="VFO72_04020"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9478483.1"
                     /translation="MSIILVGVNHKTAPVEIRERLAFSDEACADGLRRLVDGEVVREG
                     LIVSTCNRVEILSATTAEQLETGTGRMAQFLDAAGELPSGFLNNHLYRHTNEEAVRHL
                     FRVASSLDSMVVGEPQVLGQVRHAYSVAVEAGTAGRVLNRLVHHTFRVAKRVRNETGI
                     AANAVSISYMAVELGKKIFDSLKGCTVLLIGAGEMAELSARHLVNAGASRVIIANRTE
                     ASARQIADEFGATTVPFDRLDQALSEADVVICSTGAPGYVLTESQARHALGRRRNRPT
                     CFIDISVPRNIDPAVGKVPNVFLFDIDDLES"
     gene            complement(949..1761)
                     /gene="ccsA"
                     /locus_tag="VFO72_04025"
     CDS             complement(949..1761)
                     /gene="ccsA"
                     /locus_tag="VFO72_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9478484.1"
                     /translation="MRSLLLAVLAAYLIAAIHSILAFINKRRSVQRVAEWSIAAGFIL
                     HTGALIADWVIDGHYPLFALRETLSFLAWALVPVYALVLYLYRAQAVGAFTMPLISAL
                     TFISLLITPGPSNAPASFSATRLFPIHTTLVIFAYAAFFIVFMASVMYLLQERELKLK
                     TFGAIFYRLPPLTTINEIATNAAVVGLTLLTLGIATGMFWSWNRYGRLWHNDPKEIFA
                     VLTWLLYLSLIVYRYTASWRGRRAAWLGVVGFALVLCTLFGARFLGGYHVFG"
     gene            1919..3103
                     /locus_tag="VFO72_04030"
     CDS             1919..3103
                     /locus_tag="VFO72_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478485.1"
                     /translation="MAQDVETIKIDTNLVTVPVIATDATGTYVADLRQDEFSLSEDGV
                     AQQIAFFGKVSMPFHVVLMLDTSSSTQDKLHLIQQAAFTFVQQLQPADRVKVVSFDDS
                     VRELNEFTNNRETLRTAIYGTRSGQGTKVYDAVELALNTIRRIQGRKAIVIFTDGMDW
                     HSDKATFNGTVRWLDEEGAIVYPIRYDTRATTERLAREQTEGPQLPTIGVIRQTPPSG
                     TTPKTFPSDDSIPTSGSEPKTGPFGLPSAEEILRRRREQERRRQPNPNDPFPEPPQRS
                     PERRAPSVPGSTTTTTTTSRRTSDDSIGRMLDMAYDTADKYLETLAEKSGGRLLRADT
                     LDSLPNAFAQIAAELRTQYLLGYYPLNKERDERYRKIKVTATRKNVVLRARPGYLASA
                     AR"
BASE COUNT          748 a          896 c          914 g          627 t
ORIGIN      
        1 gactcgagat cgtcgatatc aaacagaaag acgtttggaa ctttgcccac ggccggatcg
       61 atgttgcgcg gcacgctgat gtcgatgaag cacgtcggac ggttgcgcct gcgtcccaac
      121 gcgtgtcgcg cttgtgattc cgttaagacg taccccggcg cgcccgtcga acagattacg
      181 acgtcagctt ccgagagcgc ctggtccaac cgatcgaagg gcacagttgt ggcgccaaac
      241 tcgtctgcga tctggcgcgc agacgcttcc gtgcgattcg caatgattac acgcgacgca
      301 cctgcgttaa cgagatgccg cgctgacagt tcagccatct cacccgcgcc gatcagcaac
      361 accgtgcagc ctttcaacga gtcgaagatc ttctttccga gctcgacggc catgtaactg
      421 atcgacaccg cattcgctgc aatgccggtc tcgtttctga ctcgcttggc gacgcgaaac
      481 gtgtggtgca ccagccggtt gagaactcga ccggcggttc cggcctcaac cgcaactgaa
      541 tacgcgtgcc gcacctgccc aagcacctgc ggctcgccaa cgaccatcga gtcgagcgaa
      601 gaagcgacgc ggaagaggtg acgcactgct tcttcgttag tgtgtcgata cagatgattg
      661 ttcaggaacc cggagggcaa ctcgcccgcg gcgtcgagaa actgcgccat gcgtcccgtt
      721 ccagtctcca actgctcggc cgtcgtcgcg ctgagtatct cgacccggtt gcacgtcgaa
      781 acgatcaatc cttcgcgcac tacttcgccg tcgacgagcc ggcgcagtcc atcggcgcac
      841 gcttcgtcgc tgaaggcgag ccgctcgcgg atttcaaccg gagcggtttt gtgattgact
      901 ccaacaagaa tgatcgacat ggaattcgac gtgaccttca atcttcaact acccgaatac
      961 gtgatacccg ccgagaaacc gggcgccgaa aagtgtgcaa agcacgaggg caaatccgac
     1021 gacgcccaac cacgcggccc ggcgtccgcg ccagctcgcg gtgtaacgat agacgatgag
     1081 actgagatag agtagccacg taagcactgc aaagatctct ttcgggtcgt tatgccagag
     1141 tcgtccataa cgattccacg accagaacat tccggtcgca atgccgaggg tcaggagtgt
     1201 caatccgaca acggcagcgt tggtggcgat ttcgttaatg gttgtgagtg gtggcagacg
     1261 ataaaagatg gcgccgaacg ttttgagctt cagttcacgt tcctgaagca gatacatcac
     1321 actagccatg aagacaatga aaaatgcggc gtaagcgaag atcacaagcg ttgtgtgaat
     1381 cggaaacagc cgcgtggcgg aaaatgatgc cggcgcgttg cttggcccag gcgtaatcag
     1441 cagcgaaatg aaagtcagcg cggaaatcaa cggcatcgtg aatgcgccga ccgcctgcgc
     1501 gcgatacagg tagagtacga gcgcgtaaac aggaacgagc gcccaggcga ggaaagacag
     1561 cgtctcgcgc agcgcgaaca agggatagtg accatcgatt acccaatctg cgatcagggc
     1621 gcccgtgtgg agtataaacc cggccgcgat cgaccactcc gcgacgcgct gcaccgaccg
     1681 gcgtttgtta ataaacgcca ggatggagtg aatggcggca atgagatatg ccgctaaaac
     1741 tgcaagcaaa agggatctca tcggggaact gatacagaac tgcgatttta tgctctagtc
     1801 ggattagagg gcaaacgtta ttcttgccga ggacatcgta gccacgcaaa tgccttctcc
     1861 taccttccat cgtgtcgttt ccatagctct aatcattttc gtgagtttgt gtttatctgt
     1921 ggctcaggac gtagaaacga taaagatcga cacgaacctg gtgacggtgc cggtgattgc
     1981 cacagacgcg accggtacct atgtggctga tttgcggcag gacgagttca gcctgagcga
     2041 agatggcgtg gcgcagcaga ttgccttctt tggcaaagtg tcgatgccgt ttcacgttgt
     2101 gctgatgctg gatacaagct cgagtacaca ggacaagttg cacttgattc agcaggcggc
     2161 gtttacgttt gtgcaacagt tgcaaccggc agatcgcgtg aaggtggttt cgtttgacga
     2221 ttccgtacga gaactgaacg agtttactaa caaccgggaa acgttgcgca ccgcgatcta
     2281 cggaacgaga tcagggcagg gaacgaaggt ctatgacgcg gttgagctgg cgttgaatac
     2341 gatccggcgg atacagggtc gcaaggcgat tgtgatcttt actgacggaa tggattggca
     2401 cagtgataag gcgacgttca atggcactgt gcgctggctc gatgaagagg gtgcaatcgt
     2461 ttatccgatt cgttacgaca cgcgcgctac cactgagcga ctggcgcgcg aacaaactga
     2521 aggaccgcag ttgcccacga tcggcgtgat ccggcaaacg ccgccgagcg gaaccactcc
     2581 caaaactttt cccagcgacg actcgatacc gacgtcagga agtgaaccga agactggacc
     2641 attcggattg ccttcggctg aagagatcct gcgccggcgc cgagagcaag agcgccgccg
     2701 gcagccgaat ccgaatgacc catttcctga gccgccgcag cggtctccgg aacggcgcgc
     2761 ccccagtgtg ccagggtcga cgactactac caccaccacg tcgcgtcgaa cgagcgacga
     2821 ttcgattgga cggatgttgg atatggctta cgacaccgcc gataagtacc tggaaacact
     2881 tgctgagaaa tccggcggta gactactgcg cgccgatacg ctcgactcgt tgcccaacgc
     2941 atttgcgcag attgcggccg aactccgtac gcagtacctg cttggttatt atccgctgaa
     3001 caaagaacgt gacgagcgtt accgtaagat caaagtgacg gcgacacgaa agaacgtggt
     3061 tctccgcgcc cgccccggct acctcgccag cgccgcacgt taagccggaa acccacggat
     3121 tacacagatt gccatttttc acgctgtgca atctgttgat ggcttgcgtg cttgttctat
     3181 ccctc
//
LOCUS       DASTEY010000224         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1422257, whole genome shotgun sequence.
ACCESSION   DASTEY010000224 DASTEY010000000
VERSION     DASTEY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422257"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2104)
                     /locus_tag="VFO72_04035"
     CDS             complement(<1..2104)
                     /locus_tag="VFO72_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9478486.1"
                     /translation="MRKVARALGAVSLSLCFALQPAIAQTPDVIDSDEFFRRNNQIRP
                     LPDYSQKIEALLKRMTLEEKVGQMTQLAIGMIASGADQNIKIDPAKLEKAIVKYGVGS
                     ILNVSDQALTIDHWHDFIRQIQEAATRKTRLGIPVIYGIDSIHGANYVQGATLFPQEI
                     GMAATWNPELMKRAAEITAAETRAAGIPWSFSPVLDLGRNPLWPRFWETFGEDPYLAS
                     VMGVAFTRGLEGTDVSSQTQVASSLKHYMGYSFPLTGRDRTPAWIPENYLREYFLPPF
                     AASIKANARTIMINSGEINGVPGHVNRHLLHGILRDELGFNGFVVSDWEDIKKLVTIW
                     RIAATEKEATRLAVMAGIDMSMVPNDYSFADHLIALVKEGAVPQARIDEAVRRILRVK
                     YELGLFENPGPNAALKANFARPESRQVSLEAARESITLLKNANNLLPLAKTAKVLVTG
                     PTADSLISLNNGWTYVWQGSEEALYPKDRDTIRRAVERKIGAGNVTYVPGTKIFRQPG
                     STSNNNPTSLEEEVDIPAAARAAQSADVVVLALGEGSYCETPGNITDLTIGEPQLKLA
                     EAIIATGKPVVLVLVEGRPRVINQIADRVPAIVMAYNPSNEGGTAIADVLFGDVNPSG
                     KLPFTYPRTPNGLINYDHKPFETENTAFGNMAFKPQFAFGEGLSYTTYAYRDLKLDKP
                     IITDKDELGVTVTVTNTGQ"
     gene            complement(2147..>2929)
                     /locus_tag="VFO72_04040"
     CDS             complement(2147..>2929)
                     /locus_tag="VFO72_04040"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET9478487.1"
                     /translation="EGADPCHLTVDQKRKSLLVANYTGGNLAVLPIQRDGGLGPAVDV
                     EQHEGSGPREQQKSPHAHCIKLDRADRFAFSADLGSDKVMIYRFTGKLEPAQQPSAKL
                     HPGAGPRHLTFHPNGRYLYAINELDSSVTTFKYDAAKGTLAAFETVSTLPRDFKGTSY
                     CADVHVSRSGRFLYGSNRGHNSIVVFAIDPHTARLKLVEHVSTEGKWPRNFVIDPSGR
                     FLLVANQHTDNVVVFHIDAQTGRLTPAGQIAEIPVPVCLQFL"
BASE COUNT          598 a          800 c          844 g          687 t
ORIGIN      
        1 gctgacccgt gtttgtaaca gttacggtga ccccaagctc atccttatcc gtgatgatcg
       61 gcttgtctaa ttttaaatcg cggtacgcat aggtcgtgta actcaatccc tctccaaaag
      121 cgaactgcgg tttgaaagcc atgttgccga acgcggtgtt ctcggtttcg aatggtttgt
      181 ggtcgtaatt gatcaggccg ttcggtgtgc gcggataagt gaagggaagt tttccactcg
      241 gattcacatc accaaacagg acgtcggcga tcgccgttcc gccttcattg ctcggattgt
      301 aggccatcac gatcgccggg acgcgatcgg cgatctgatt gatgacccgc gggcggcctt
      361 cgaccaaaac cagcacgacc ggctttccag tcgcgatgat tgcttcagcg agtttgagct
      421 gcggttcacc gatcgttagg tcggtaatat ttcccggcgt ttcacagtaa gacccttcgc
      481 cgagagcgag cacgaccaca tccgcggatt gcgcagcacg ggccgccgct ggaatgtcga
      541 cctcttcttc cagacttgtc ggattgttgt tggaagtcga gccgggctgc cgaaagattt
      601 tcgtgcccgg aacataagtg acgttacccg cgccgatctt cctttcgacc gcgcgacgaa
      661 tcgtgtcgcg atcttttgga tacagcgctt cttccgatcc ctgccaaacg taagtccaac
      721 cgttgttgag cgagatcaac gagtcggcgg ttggtccagt gacgagcact ttcgccgtct
      781 tcgccagcgg caacaagttg ttcgcatttt tgagcagcgt gatcgactcg cgagctgctt
      841 ccaaactcac ctgccgcgac tcggggcgcg caaagtttgc tttgagcgcc gcgttcgggc
      901 ccggattttc aaacaggccc aactcatact tcactcgcag aatcctacgc accgcttcat
      961 cgatacgcgc ctgcggcacc gcgccttctt tcaccagcgc aatcaggtga tcggcaaaac
     1021 tgtaatcgtt cggcaccatg ctcatgtcga tgcccgccat cacggcgaga cgcgtcgcct
     1081 ctttttcagt cgcggcgata cgccagatgg tgacgagttt cttgatgtct tcccaatcgg
     1141 aaacgacgaa gccattgaag ccgagttcgt cgcgcagaat gccgtgcagc agatggcggt
     1201 taacgtggcc gggcacgcca ttgatctcgc cggagttgat catgatcgtg cgtgcgttgg
     1261 ctttgatcga ggcggcaaac ggcggcagaa aatactcgcg caggtaattc tctggaatcc
     1321 acgccggcgt gcgatcgcgt ccggtgaggg gaaaactgta tcccatgtaa tgtttcaaac
     1381 tcgacgcaac ctgcgtttgc gacgagacgt ccgtgccttc gagtccacgc gtaaacgcga
     1441 cgcccatcac tgatgcaaga taaggatcct cgccaaacgt ttcccagaaa cgcggccaaa
     1501 gcggattgcg tccgaggtcc agcacgggcg agaagctcca gggaatgccc gcggcacgtg
     1561 tctcagccgc agtgatctcg gcggcacgtt tcatcagttc ggggttccat gtcgcggcca
     1621 taccaatctc ttgcggaaag agtgtggcgc cctggacgta gttcgcgccg tgaatcgagt
     1681 cgatgccgta gatgacggga atgccgagtc gggtcttcct ggttgcagct tcctggatct
     1741 ggcggatgaa gtcgtgccag tgatcgatcg tgagtgcctg atccgagacg ttgagaatcg
     1801 atccaacgcc gtacttaacg atggccttct ccaactttgc cggatcgatc tttatgttct
     1861 gatcggcgcc cgaagcaatc atgccgatgg ctaattgcgt catctgcccg actttctctt
     1921 cgagcgtcat gcgtttgagc agggcttcga tcttttgcga atagtctggc aacgggcgga
     1981 tttgattgtt gcgacggaag aactcgtcgc tgtcgattac gtcgggagtc tgggcgatgg
     2041 ccggctggag tgcaaaacac agtgaaagac tgactgcccc cagggcacgg gcgacttttc
     2101 tcatctattt gctttcctca ggcaggaata tcggaacgcg cctattctag agaaattgta
     2161 agcagactgg aactggaatt tctgctattt ggccggccgg cgtcagccgt ccggtctggg
     2221 cgtcgatgtg aaacacgacc acgttgtcag tgtgctgatt ggcgaccagc agaaaccggc
     2281 ccgacggatc gataacaaag tttcgcggcc acttgccttc ggtcgaaaca tgctcgacga
     2341 gcttgagtcg cgcagtgtgt ggatcgatgg caaacacgac gatactgtta tggccgcgat
     2401 tcgatccgta aagaaatcgt cctgatcgcg aaacgtgaac gtcggcgcag tagctcgtgc
     2461 ctttgaaatc gcgcgggagc gtggaaacgg tttcaaacgc ggccagagtt ccttttgcag
     2521 cgtcgtattt gaaagtggta actgaggaat cgagctcatt gatcgcgtat agatatctgc
     2581 cgtttgggtg gaacgtaagg tgacgtggtc cagcgccggg atgaagctta gccgaaggtt
     2641 gttgcgccgg ctcgagctta ccagtaaacc ggtagatcat caccttgtcg ctaccgaggt
     2701 ctgcggaaaa cgcaaagcgg tcagcacggt ccaacttgat gcaatgtgcg tgcggtgact
     2761 tttgttgttc gcgtgggcct gaaccttcgt gttgttcgac gtcgaccgcg ggaccgagac
     2821 caccgtctcg ctgaatcggc aacaccgcta agttcccgcc ggtgtagttc gcgaccagta
     2881 agctctttcg tttttgatct actgtgaggt gacagggatc tgcgccttc
//
LOCUS       DASTEY010000225         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1059828, whole genome shotgun sequence.
ACCESSION   DASTEY010000225 DASTEY010000000
VERSION     DASTEY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059828"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFO72_04045"
     CDS             complement(<1..711)
                     /locus_tag="VFO72_04045"
                     /inference="COORDINATES: protein motif:HMM:NF005594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9478488.1"
                     /translation="MSGNKAVVTGGTKGIGRAIARALIAAGVDVSISARHEDEIARTV
                     SELNELGPGRSAGVGCDVRDESQVKKLFAHAATVLGGLDILVNNAGIGIFAPVESTSG
                     EDFRAVIETNLCGVFYCCHEAIPLMKQRGGGYIFNISSLAGVNAHPEMAAYNASKFGL
                     NGFTEALMQEVRHDGIKVSYIMPGSVNTEFGGDEPSPEKSWQLQSEDVARVVMDLLQF
                     PDRALPSRIEIRPSRPPKR"
     gene            complement(725..>2118)
                     /gene="gatB"
                     /locus_tag="VFO72_04050"
     CDS             complement(725..>2118)
                     /gene="gatB"
                     /locus_tag="VFO72_04050"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET9478489.1"
                     /translation="TRFGDEPNANTCPTCLGLPGALPVLNRRAVELGARAALALGLRI
                     NRQSIFARKNYFYPDLPKGYQISQYDQPFSADGELEIMTAERDAAGHPLEWRPKKIRI
                     TRLHLEEDAGKNVHEGLPDVDRYSYIDLNRAGVPLAEIVTEPDFRSSWEAYDYVNHVR
                     RALQWVGASEADMEKGNLRCEANVSVRRVGEEKFGTKVELKNLNSVRFMQRAIEFEVA
                     RHIKVLESGGRVTQETRLWDERATETRVMRSKEEAHDYRYFPEPDLQPLVVSEEFIEE
                     VRRVMPELPERRIKRFAEEYNLSFADASQLVSERSLANYYEAAAKASGNTRAAANWIR
                     TELLRELEASGRTAAESPVPAEELGALVRLIDEAKISGKQAKDVLVEMFKTGKGALTI
                     IQEQGLVQVSDTGEIDTLIDEVIAANPDQVASYRSGKEALLGFFVGQVIKASKGKANP
                     KVVNERLRAKLSA"
BASE COUNT          419 a          664 c          563 g          472 t
ORIGIN      
        1 tctctttggg ggacgacttg gtctaatttc gatacggctc ggcagagcgc gatccggaaa
       61 ttgaagcagg tccattacga ctcgcgcgac atcctcagat tgcagttgcc aactcttctc
      121 cggactcggc tcgtcgccgc caaattcggt gttgactgaa ccaggcatga tgtaactcac
      181 tttgatgccg tcgtgacgca cttcctgcat cagcgcttca gtaaaaccat tcagaccaaa
      241 cttcgacgca ttgtaagcag ccatctccgg atgggcgttg acgccggcaa gcgacgagat
      301 gttgaagatg taaccaccgc cgcgttgctt catcaacgga atcgcctcat ggcaacaata
      361 gaacaccccg cagagattgg tttcgatcac ggcgcggaag tcttcgccac tggttgactc
      421 aacgggtgcg aagatgccga tgccggcgtt gttcacgaga atatcgagac cgcccaggac
      481 tgtggctgcg tgtgcgaaga gctttttgac ttgggactca tcgcgaacgt cgcagccaac
      541 gcctgcggag cgaccaggcc cgagctcatt caattcgctc acagtccttg cgatctcgtc
      601 ttcgtggcgc gcactgatgc tgacgtctac gccggcagca atcaatgctc gtgcaatggc
      661 ccggccgata cctttcgtgc cacctgtcac caccgctttg ttaccggaca agctcataca
      721 ccgattacgc agaaagtttc gcccgcaggc gttcgttcac gaccttagga ttcgccttcc
      781 cttttgaagc cttgatcacc tggcccacga aaaacccgag caacgcctct ttaccgctgc
      841 gataacttgc aacttgatcc gggttcgcag cgatcacttc gtcgataagt gtgtcgatct
      901 caccagtgtc acttacctgg accagcccct gctcctggat gatcgtgagc gcgcctttgc
      961 cggtcttgaa catctcgacc agcacgtcct tggcctgttt gccgctgatc tttgcttcgt
     1021 caatcagtcg caccagcgcg cccaactctt ccgcggggac cggagattcg gccgccgttc
     1081 gccccgacgc ttcgagctcg cgcaacaact cagttcggat ccagttcgca gcggcgcggg
     1141 tatttcccga cgccttcgcc gctgcttcgt agtagttcgc cagcgaacgt tctgacacca
     1201 actgcgaagc atccgcgaaa ctcagattat attcttcggc aaaccgcttt atgcgacgct
     1261 cgggcaactc cggcatcact ctgcgcactt cttcaatgaa ctcttcggag acaaccagcg
     1321 gctgtagatc gggctccgga aagtagcgat agtcgtgcgc ttcctctttc gaacgcatca
     1381 cgcgggtctc cgtcgcgcgc tcgtcccaca accgggtctc ctgcgtaacg cgccctcctg
     1441 actcgagcac cttgatgtgg cgcgcgactt caaactcgat cgcgcgctgc atgaaccgca
     1501 ccgagttgag gttcttcaac tccaccttcg taccaaactt ctcttcacca acacgtcgca
     1561 ccgacacgtt ggcttcgcac cgtaggtttc ccttctccat gtcggcttca gaggctccga
     1621 cccattggag cgcgcgacgg acgtgattga cgtaatcgta ggcttcccac gacgagcgaa
     1681 agtcgggctc ggtcacgatt tccgccaacg gcactccggc gcgattcaga tcgatgtacg
     1741 agtagcggtc gacgtccggc aaaccttcat gaacgttctt tcccgcgtct tcttcgaggt
     1801 gcagtcttgt gatccggatc ttcttcggac gccactccaa gggatgaccg gcggcatcgc
     1861 gttcggcagt catgatctcc aactcgccat cggcggagaa aggttgatcg tactgcgaga
     1921 tctgataccc cttcggcaga tcgggataga agtagttctt gcgcgcgaag atcgattgcc
     1981 gattgatgcg caagccgagc gcaagggcag cgcgggcgcc tagttcgacg gcgcgtcgat
     2041 tcaacactgg caaggcgccg ggcaaaccga gacacgttgg gcaggtgttt gcgtttggct
     2101 cgtcgccgaa acgcgtgg
//
LOCUS       DASTEY010000226         6736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1076291, whole genome shotgun sequence.
ACCESSION   DASTEY010000226 DASTEY010000000
VERSION     DASTEY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076291"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /gene="ggt"
                     /locus_tag="VFO72_04055"
     CDS             complement(<1..1681)
                     /gene="ggt"
                     /locus_tag="VFO72_04055"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9478490.1"
                     /translation="MNTLRKLGPILLILVFINSSVAPVTAREPVRGRRGMVASTNEVA
                     SRVGVDILKRGGNAVDAAIAVAFTLAVTHPAAGNLGGGGFMMIRLKNGHTTAIDYREM
                     APAAAHRDVYLDKNGNLIEGEGGSLIGYRAAGVPGTVRGMELALKKYGSGKLTWSQLV
                     EPARRLAANGFTVTHSLARSLHSSREYLSKYADTKRIYLRNGNFYREGELFRQPELAA
                     TFARLQQLGPNEFYEGETARLIAADMKRNNGLMTLEDLRGYVAKERKPVRGNYRGHEI
                     ISMPPPSSGGAVLIQMLNILEGFDLSKLEASSSDRYHLMAEAMRRAFADRAEYMGDSD
                     FVKVPIAGLIEKSYAAKLRSTINTERASSSAEVRAGRPAGYESEDTTHFAVVDVEGNA
                     VANTYTLNNSYGSAAVAKGTGLLLNDEMDDFAAKPGTPNLYGLIQGERNAVAPKKRPL
                     SAMTPTIVLHKDGSLWFTVGSPGGPTIINTVLCIITHVIDYEMNIQQAIDAPRIHHQW
                     LPDELVGEPFGLSGDTQRALTARGHTLAKKRYLGDAEGIMIEEKTGMRLGAT"
     gene            complement(1665..4223)
                     /locus_tag="VFO72_04060"
     CDS             complement(1665..4223)
                     /locus_tag="VFO72_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9478491.1"
                     /translation="MDQLVENLINSLGAIVGRENVLSKADELLVYECDGLPQHKHRPR
                     AVVFPSSTEDTSAIMRELARLKVPFTPRGAGTGLSGGALALDSGVVIEFARMRKILRI
                     DVENRLAVVQPGVVNLDVSRAVLPYDLYYVPDPSSQPSCTIGGNIAESAGGIHCLKYG
                     TTTDHVVGCRVVLAGGEVVDLGLDSAGYDLLGTFIGSEGTFGITTEATLKLSQIPPAV
                     RTLLAEFSEVDNASHAVSAIIAAGVMPAALEMMDHEIIRAVEASVFAAGLPPDAGAAL
                     LIELDGIEAGLDDEARRVTSICLEYGARNCRLARDETERKKLWAARKGAFGAIGRISP
                     DSMIQDAVVPRSRLPQVLRAAYDIAARYQLRIANVFHAGDGNLHPLICFDSRFSDQVR
                     RVKEAGRELMEVCVAAGGTITGEHGVGLDKRELLPLVFSEADMNAMLSVRAAFDPLGL
                     CNPGKIIPMLRGCGEAKAVATDDTETTDRTSLLPDLLRGQRTNIAFQTSVKSVSSVTC
                     PSSTEELSEIVKRAAAERRTITPAGGKTLLSSPTTNLVIDTTRLDRILEHEPADLIAI
                     AQAGVTLTAFNQKLAENGQWLPLDPPDEGHATLGGVVATGLGGAQQFGYGRPRGSVIG
                     MTVVLADGSVIKAGGRVVKNVAGYDLCKLFTGSYGTLGIITELIFKLRPRPAREATVV
                     AAGTIGELLSVGYAVVNVRLFPVAVELISTGSASRIGIDVEAKSAALLIRFAGNEKAV
                     AYQIDETMRCLKHSSVSSTEVLTEDSQLWRSLAALPVQESPSFVTRVLPSDLWKTIGS
                     FDSNSLWQVGVADGRIRMKNRPPDHTSLDALSQRIKQQLDPFNLLRGHDHEHAA"
     gene            4309..5952
                     /locus_tag="VFO72_04065"
     CDS             4309..5952
                     /locus_tag="VFO72_04065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9478492.1"
                     /translation="MNVPVHPDKPSLVADIIITNAVVHTMDPAQPTAEAVAIYANRIV
                     AVGSTKDIRKLAGPNTRRIDAKKRLLLPGFNDAHTHFLSGGFQLSSVDLRDANSPQEF
                     AARIKAFAEKLPKGRWITGGDWDHERWPDAKLPTKELIDSFTPDTPVFVNRLDGHMAL
                     ANSLALKLAGVTRQTLDPPGGVIVRDKSGEPTGVLKDAAQSFVWKVVSPATFDEKLAA
                     ARAATNYAAKLGVTSVQDMSAGTDVGVYQTLLDRGELKTRIYAVSPLPAWERLARTGM
                     RAHFGSEMLRVGGLKGFADGSLGSTTALFYEPYRDDPSTSGIAGDEMYPEGAMLKRVT
                     EADRAGLQVMIHAIGDRANDLILGLFEQVERENGKRDRRFRIEHAQHLRTQDIARFKL
                     DQVIASMQPYHAIDDGRWAEKRIGKERAKTTYAFRSLLDSGATLAFGTDWTVAPLNPM
                     LTIYAAVTRRTIDGKNSKGWVPEQKISVAETVRAYTAGSAYAEFQENVKGTITTGKLA
                     DLVLLSRDIFKIDPKEIENVKAVLTMVDGRVVYEERDRE"
     gene            complement(5928..>6736)
                     /locus_tag="VFO72_04070"
     CDS             complement(5928..>6736)
                     /locus_tag="VFO72_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9478493.1"
                     /translation="PIILFIGAGLFFLNPQAFTLGPSPNSSAFSQSVLLLVYAFTGFE
                     MATIPAGEVRDPQKNLPRALLITILVVAVVYILIQIVCVGTLPGLAQSQRPLADAGSQ
                     FMGTAGGAIISAGAIISITGNLNILMLSGSRLPFAMAEQKQLPAFIGSVHRSFFTPWV
                     AILITAGLMLFLTLKSSFVAALTISTIARLVTYGATCIALPVLRRRKDGPPAAFKLPG
                     GTIVALLSLVLIIWLLGHSTLAEAEATAWAAGVGLVIYLAFWSYSRSRSS"
BASE COUNT         1457 a         1976 c         1922 g         1381 t
ORIGIN      
        1 ccgtcgcgcc gagccgcatg ccggttttct cttcgatcat gattccttcg gcatcgccga
       61 gatagcgctt cttagccagc gtgtgcccgc gcgcagttaa agcgcgttgc gtgtcgcccg
      121 ataaaccgaa gggctctccc accagttcat ccggcaacca ctgatgatga attcgcggcg
      181 cgtcgatcgc ctgctggatg ttcatctcgt aatcgataac gtgggtgatg atacagagca
      241 ccgtattgat aatcgttgga ccaccgggac tgccgacggt aaaccacagg ctcccatcct
      301 tgtgcaacac gatcgtcggc gtcatcgcag acagcggccg tttctttggc gcgactgcat
      361 tgcgctcgcc ctgaatcaac ccgtacaggt ttggcgttcc gggtttcgcg gcgaagtcgt
      421 ccatctcgtc attgagcagg agccccgtgc ctttagctac cgccgccgaa ccgtacgaat
      481 tgttcagcgt ataggtgttg gcaaccgcgt ttccttcgac atcgacgacg gcgaagtgag
      541 ttgtgtcttc cgattcataa ccggccgggc ggcccgcgcg cacttcggca ctactcgacg
      601 cgcgctcagt gttgatggtc gaccgcaact tagccgcgta agacttctcg atcagcccgg
      661 caatcggcac cttcacgaaa tcggagtcgc ccatgtactc cgcgcgatcg gcaaacgcgc
      721 gccgcatcgc ttcagccatc agatgatacc gatccgacga cgacgcttcg agtttactga
      781 gatcgaagcc ttcgagaatg ttgagcatct ggatcagaac cgcgccgcct gaagacggag
      841 gcggcatcga gatgatctca tgcccgcgat agttaccgcg caccggcttg cgttccttag
      901 cgacgtagcc acgcaagtct tccagcgtca tcaacccgtt gtttcgtttc atgtccgcag
      961 caatcagccg agccgtttcg ccttcgtaaa actcgtttgg accaagttgc tggagtcgag
     1021 cgaaggtcgc ggccagttcc ggttggcgaa acagttcgcc ttcacgatag aagttgccgt
     1081 tgcgcaaata gattcgtttt gtatcggcgt actttgaaag atactcgcgg ctggaatgga
     1141 gtgaacgcgc cagggaatgc gtgacggtga agccgttcgc ggcgagccgg cgcgcaggtt
     1201 cgaccaattg gctccaggta agttttccgg aaccgtattt cttgagcgcg agctccatgc
     1261 cgcgaaccgt gccaggcacg ccggctgcgc gatagccgat aagtgaaccg ccttcacctt
     1321 cgatcaggtt tccattcttg tcgagataga cgtcacgatg agccgcggcc ggcgccatct
     1381 cgcgataatc gatggccgta gtgtggccgt ttttcaagcg aatcatcatg aagccaccgc
     1441 caccgagatt gcccgcggcg ggatgagtaa cagcgagcgt gaaagcgact gcgatcgcgg
     1501 cgtccacagc gttgccgccg cgcttcagga tgtcgacgcc gacacgcgat gcgacttcat
     1561 tcgttgacgc caccatgccg cgtctgccgc gcaccggttc gcgcgccgtg accggagcca
     1621 cggatgaatt aataaagacc agaattaaaa ggattggtcc gagtttacgc agcgtgttca
     1681 tggtcgtgtc ctcgcagtaa gttgaaagga tccagttgtt gcttgatgcg ctggctgagc
     1741 gcgtcgagtg atgtgtgatc gggtggccga ttcttcattc taattcggcc gtctgccact
     1801 cccacttgcc aaagagaatt actgtcgaac gagcctatcg ttttccacag gtctgaaggt
     1861 aacactcggg ttacaaaaga cggggactcc tgtaccggca aggcggcgag tgatcgccag
     1921 agctgtgaat cttcggttag aacttcggtc gagctcacgg acgagtgctt caaacaacgc
     1981 attgtctcgt cgatttggta agcaaccgcc ttttcgttgc ccgcgaagcg aatcagcagc
     2041 gctgcggatt ttgcttcaac gtcaatgcca atccggctgg cggaccccgt tgatatcaac
     2101 tccactgcta ccgggaatag tctaacgtta acaacagcat agccaactga cagtagttcg
     2161 ccgatcgtac ccgcggcgac tacagtcgct tcacgcgccg ggcgtggccg cagtttgaaa
     2221 ataagttcgg tgatgatccc caacgtgccg taactgcccg tgaacaactt acagagatcg
     2281 taaccggcca cattcttcac cacgcgcccg cccgctttga tcacgctgcc gtcagccagc
     2341 accacggtca tgccgatgac cgagccgcgt gggcggccgt aaccaaactg ctgtgcgcca
     2401 ccgagaccag tcgcgacgac accgccaagc gttgcatgtc cttcgtccgg aggatcgagt
     2461 ggcaaccact gtccgttctc ggcaagcttt tgattgaagg cggtgagtgt tacgcctgct
     2521 tgcgcgatcg cgatcaggtc tgctggttcg tgttcgagta ttcgatcgag acgggttgtg
     2581 tcgatgacaa gattggtcgt aggactactc aacaacgtct tgccgccggc aggtgtgatg
     2641 gtccgacgtt cggccgcagc acgtttcacg atctcggaga gttcttcggt ggaggatgga
     2701 caagtcacgg atgaaacgga ttttacggac gtttgaaacg cgatgttagt acgttgtccg
     2761 cgtaaaaggt caggcagaag tgaagtccta tccgtggttt ccgtgtcatc cgtggccaca
     2821 gccttcgctt cgccgcagcc gcgcagcatt gggatgatct tgccggggtt gcagagaccg
     2881 agcggatcga aggcggcacg cacactcagc attgcgttca tatcggcttc ggaaaaaaca
     2941 agcggcagca attcgcgttt atccaatccc acgccgtgct cgccggtgat cgtgccgccg
     3001 gcggcaacgc agacctccat taactcgcgc cctgcttcct tgacgcgcct cacttgatcc
     3061 gaaaaacgtg agtcgaaaca gatcaacgga tgcagattgc catcgcccgc gtgaaacacg
     3121 tttgcgattc gcaactgata gcgcgcggcg atgtcgtaag cggcccgcaa cacttgggga
     3181 agacgcgagc gcggaacaac cgcatcctgg atcatcgaat cgggcgagat acgcccgatc
     3241 gcgccaaatg cgcccttgcg cgcggcccac agttttttgc gttcagtctc atcgcgcgcg
     3301 aggcggcagt tgcgcgcgcc gtattccaaa cagatgctcg ttaccctgcg tgcttcgtcg
     3361 tcgagtcccg cttcaattcc gtccaactcg ataagcagcg ccgcgcccgc gtcgggtgga
     3421 agcccagccg cgaacacact cgcctcgacc gcgcgaatga tctcgtgatc catcatctcg
     3481 agtgccgccg gcatcacgcc cgcggcaata atggccgaca ctgcatgact cgcattatca
     3541 acttcactaa actccgcgag cagcgtccgc accgcgggag gaatctggct cagtttgagc
     3601 gtcgcctcag tagtaattcc gaatgttcct tcggagccga taaaggtgcc gagtaggtcg
     3661 taacccgctg aatccaatcc aagatccacg acctctcctc cagccagcac tacccgacag
     3721 ccgactacgt ggtcggtcgt ggtgccgtac ttcagacaat gaatgccacc cgcactctcc
     3781 gcgatgttgc caccaatcgt gcaactcggc tgactcgacg gatctggtac gtaatacaga
     3841 tcgtacggca acaccgctcg cgagacatcc agattcacga cacctggttg cactaccgcc
     3901 agacgatttt caacatcgat gcgcaatatt ttgcgcatgc gtgcaaactc gattaccacg
     3961 cctgaatcga gcgcgagcgc cccaccggac aatccggttc cggccccgcg cggcgtgaag
     4021 ggaaccttga ggcgcgcgag ttcgcgcatg atcgcggagg tgtcttcagt tgaagaagga
     4081 aagacaacgg cgcgcgggcg atgcttgtgc tgcggcagac cgtcgcattc gtagactaac
     4141 agctcgtcag ctttggaaag gacattttcg cggcctacga tcgcgcccag actatttatt
     4201 agattttcga ctagttgatc cacgccccat ttgtcaacag tgtacaatct accgcttatg
     4261 aaaaccacac cgctgcttgt tccgatcctg gctttggcgc tgaatcttat gaacgttcct
     4321 gtccaccctg ataaaccttc gctcgtagcc gacatcatca taaccaacgc cgtggtacac
     4381 acgatggatc cggcgcaacc aaccgcagaa gctgtagcga tctacgcgaa ccggatcgtc
     4441 gcggtcgggt cgactaagga catcaggaaa ctcgctggtc caaacacgcg caggattgac
     4501 gcaaagaagc gcttgctgct ccccggtttc aacgacgcgc acacacattt tctcagcggc
     4561 ggctttcagc tttcgagtgt ggacctgcgt gacgcaaact cgccgcagga gtttgcggcg
     4621 cgtatcaaag cctttgccga gaagttgccg aagggtcgtt ggatcaccgg cggtgattgg
     4681 gaccatgaac gctggcccga tgcgaaactt ccaaccaaag agctgatcga cagtttcact
     4741 cccgatacgc cggtgttcgt caaccggctc gatggacaca tggcgctcgc aaacagtttg
     4801 gccctcaagc ttgcgggcgt cacacgtcaa actctcgatc cgcccggcgg cgtgatcgtt
     4861 cgcgataaat ccggcgagcc gacgggcgtg ctcaaagacg cggcgcagag ttttgtgtgg
     4921 aaggtcgtat cacccgcgac gttcgacgag aaacttgccg ccgcgcgcgc cgcgacaaac
     4981 tatgccgcga aactcggcgt cacgagtgtt caggacatgt ctgccggtac tgacgtcggc
     5041 gtctatcaaa ctcttttgga tcgcggtgag ttgaagacgc gaatctatgc ggtgtcgcca
     5101 ttgccggcgt gggagcgtct ggcccgcacg ggcatgcgcg cgcacttcgg gagcgaaatg
     5161 cttcgtgttg gcgggttgaa aggttttgcg gacggcagtt tgggttcgac gaccgccctc
     5221 ttctacgaac cttaccgcga cgatccatcg acgagtggca tagccggtga cgagatgtat
     5281 cccgagggtg cgatgctcaa acgcgtcacg gaagcggatc gcgccggtct gcaagtaatg
     5341 attcacgcga tcggcgaccg ggccaatgat ctaatcctgg gcctcttcga gcaggtcgaa
     5401 cgcgaaaacg gcaaacgcga tcggcgcttt cgcatcgagc acgcgcagca cctgcggacg
     5461 caggacatcg cgcgtttcaa actcgaccag gtgatcgcgt ccatgcagcc gtatcacgcc
     5521 atcgacgatg gccgctgggc cgagaagcga atcggcaaag aacgcgcgaa gactacctac
     5581 gcatttcgtt cgttgctcga ttccggcgcg acgctggctt tcggtactga ttggactgtc
     5641 gcaccgctca atccgatgtt gacgatctac gctgccgtaa ctcgccgcac gatcgacggc
     5701 aagaattcaa aaggctgggt gcccgagcaa aagatctcgg ttgcggagac tgtgcgcgct
     5761 tacactgcag gttccgcgta cgcagagttt caagagaacg tgaagggaac tatcaccacc
     5821 ggcaaactcg ccgatctcgt actgctctcg cgcgacatct tcaagatcga tccgaaggag
     5881 atcgaaaacg tgaaggccgt gttgacgatg gttgatggcc gggtggttta cgaagaacgc
     5941 gaccgcgaat agctccagaa ggccaggtaa atcacgagac ctaccccggc ggcccacgct
     6001 gtggcttcag cctctgccag tgtgctgtga ccgagtaacc agatgatcag cacgagcgac
     6061 agcaacgcga cgattgtgcc gccgggtagt ttgaacgccg cgggtggtcc atccttgcgc
     6121 ctgcgcagga caggcaaagc aatacacgtc gcgccgtagg tgacgagacg cgcaatagta
     6181 ctgatcgtca gcgctgccac gaacgaactt ttcagtgtga ggaaaagcat caagcctgcg
     6241 gttatcaaaa tcgccaccca gggcgtgaag aaggatcggt gaacgctacc gatgaaagcc
     6301 ggcaactgtt tttgttcggc cattgcgaac ggcaggcgcg aacccgagag catcagaatg
     6361 ttcaagttac cggtaatcga gatgattgcg ccggccgaga tgattgcgcc gcccgctgtt
     6421 cccataaatt gtgagccggc atcggcgagt ggcctctgcg attgggccag accgggcaaa
     6481 gttcctacgc agacaatttg aatcaggatg tacaccacag cgacgacaag gatggtgatg
     6541 agcaatgctc gcgggaggtt tttctgcggg tcgcgcactt cacccgccgg aatagtcgcc
     6601 atttcaaatc cggtaaaggc ataaaccagc agtaaaacag actgcgaaaa tgcactcgag
     6661 ttcggacttg gtccaagcgt aaaggcttgc gggttgagaa agaacaggcc ggcgccgatg
     6721 aaaaggataa tcggca
//
LOCUS       DASTEY010000227         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_103711, whole genome shotgun sequence.
ACCESSION   DASTEY010000227 DASTEY010000000
VERSION     DASTEY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103711"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFO72_04075"
     CDS             <1..571
                     /locus_tag="VFO72_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478494.1"
                     /translation="DEVRGRRAVVALTDGVDSASNSDFATAKVKLSRAGVACYFVQIN
                     TEDFVEDRLMKDCQDDGQLSLSQRQIERYRRLFVPAAKAEDFNDFCRMGPFERMSISR
                     ELYNLARREMNDLARVSGGRSFPAATLADARAAFARVAADIGTLYSLGYYPTNKARDG
                     KFRTIKLEVKGVKEKAQIRARDGYNAPKQ"
     gene            584..1771
                     /locus_tag="VFO72_04080"
     CDS             584..1771
                     /locus_tag="VFO72_04080"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET9478495.1"
                     /translation="MPNPFNRILLVVLDGAGIGAMPDAPEWGDAGSDTFGHILESREV
                     RLPNLQRYGLGNIRPLAKLPPINNPVGSYGRCALRSNGKDTTTGHWEMAGIILERAFP
                     TYPDGFPQTIIDRFVREADVPGILGNIPASGTEIIKVLGEEHVKTRKPIVYTSADSVF
                     QIAAHEEVIPLERLYEMCEVARRILDGEHKVGRVIARPFLGEPGSFYRTENRHDYAVP
                     PPRENLLPALADADLDVVCIGKIASIYDSLGVTQDLTAKNNEQSITQTIKALGARTRG
                     LIFSNLVDFDMLYGHRRDTEGYAKALEHFDTRLPEIEAAMRADDLMMITADHGNDPTF
                     PGSDHTREYAPLLVFGKSARPGVNLGTRPSLADIGQTIADNFSLQLAAGTSFLTELRA
                     AAD"
     gene            complement(1782..>2466)
                     /locus_tag="VFO72_04085"
     CDS             complement(1782..>2466)
                     /locus_tag="VFO72_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9478496.1"
                     /translation="IRASLKTLGQGKLRFILNTHWHGDHTGGNAAFGPEATIIAHDNV
                     RKRMSTEQRLEFFKQTVPASPKEALPVITFDQSLAVHFNGEEIRALHLPQGHTDGDSI
                     IFFTSSNVVHLGDDFFSGSFPFVDLDSGGSVEGLTKNISELIPKIPAGAKLIPGHGPV
                     STIDDLKTFHRMLTETTAIVRQKIAAKKTLAQIKSEGLPAEWDSWGKAFIKTDQWIEL
                     IHRSLTMNK"
BASE COUNT          516 a          700 c          706 g          544 t
ORIGIN      
        1 ggatgaggtg cgtggcaggc gagcggtggt ggcgttgact gatggcgttg actctgcgag
       61 caactcagat tttgcgactg cgaaagttaa actctcgcgc gcgggtgttg cgtgttactt
      121 cgtgcagatc aacacggaag actttgttga agatcgtctg atgaaagact gccaggacga
      181 cgggcagctt tcgctttcgc aaagacagat cgagcgttac cggcgattgt ttgtgcctgc
      241 cgcgaaggcc gaagacttca acgacttttg ccgcatgggc ccatttgagc gaatgtcgat
      301 cagccgcgag ctttacaatc ttgcgcgacg cgaaatgaat gacctcgcgc gtgtctccgg
      361 tgggcgaagt tttcccgctg caacgttggc tgatgcgcgc gcggcgtttg cacgcgtggc
      421 agccgacatc ggcacgcttt acagtcttgg ctattatcca accaacaaag cgcgcgacgg
      481 gaagtttcgc actatcaagc tcgaagtaaa gggagtgaaa gagaaggccc agattcgcgc
      541 gcgcgacgga tacaacgcgc cgaagcagta actcccatca taaatgccaa atcctttcaa
      601 ccgtatctta ctcgtcgttc tcgacggcgc cggaatcggt gcgatgcctg atgcgcctga
      661 gtggggcgac gctggttcag acacgtttgg tcatattctc gagtcgcgcg aagtgcgtct
      721 cccgaatttg cagcgttacg gtctgggcaa tattcgacca cttgcaaaac tgccacccat
      781 caacaatccg gttggctcct acggccgctg tgctttgcgt tcaaacggca aagacacaac
      841 cactggccat tgggagatgg ccggaataat tcttgagcgc gctttcccga cttatcccga
      901 tggctttccg caaacgatca tcgaccgctt tgtgcgtgag gctgacgtgc ccggcatact
      961 cggcaatatt cctgcttcag gcaccgagat catcaaggtg ctgggcgaag agcacgtgaa
     1021 gacgcgtaag ccgatcgtct acacgtcggc cgattctgtt tttcaaattg ccgcgcacga
     1081 agaggtgatt ccgctcgaac ggctttacga aatgtgtgaa gtcgcgcgcc ggatactcga
     1141 tggcgaacac aaagtcgggc gcgtaatcgc tcgtccgttc ttgggcgagc cgggctcgtt
     1201 ttatcggact gagaaccggc acgattatgc ggtgccgccg ccgcgcgaga accttttacc
     1261 ggcgttggct gatgccgatc tcgatgtggt gtgtatcggt aagattgcgt cgatctacga
     1321 ttctcttggc gtgactcagg atctcaccgc gaagaataac gagcagtcga tcacgcagac
     1381 gattaaggcg cttggcgctc gcacacgcgg gctgatcttt tccaacctcg ttgatttcga
     1441 catgctttat ggccaccggc gcgacactga aggctacgcc aaagcactcg aacacttcga
     1501 cacgcgtctg ccggagatcg aggcggcaat gcgcgccgac gacctgatga tgatcaccgc
     1561 cgatcacgga aacgacccga cgtttccggg ctcagatcac acgcgcgaat acgcgccatt
     1621 gctggttttc ggcaagtctg cccgtccggg cgtcaacctc ggcacccgcc cctccctcgc
     1681 cgacatcggc caaaccatcg ccgacaactt cagcctccag ctagcggccg gaacaagctt
     1741 cctgacggaa ctacgagcag ccgcggatta gatcgcggct cttacttgtt catcgtcagg
     1801 ctgcggtgga tgagctcgat ccactggtcg gttttgatga aggccttccc ccaggaatcc
     1861 cactcggccg gtaagccttc gctcttgatc tgggccaggg ttttcttggc cgcgatcttt
     1921 tggcggacga tcgcggtcgt ttccgtgagc atgcggtgaa aggtcttgag atcgtcgatc
     1981 gtggaaaccg gaccatgacc ggggatcagt ttcgcacccg cgggtatttt cggaattaac
     2041 tcgctgatgt ttttggtcaa tccttccaca ctgccgccgc tatccaggtc aacaaatgga
     2101 aaactcccgg agaagaagtc atcgccgagg tggaccacgt tcgacgaagt gaagaagatg
     2161 atgctgtcgc cgtcagtgtg tccctgcggc aggtgaagcg cccgtatctc ttcgccgttg
     2221 aagtgcacag ccagcgactg atcgaacgta atcaccggca gcgcttcctt tggcgacgcc
     2281 ggaacggttt gcttaaagaa ttccaaccgc tgttcagtgg acatgcgttt gcgcacgtta
     2341 tcgtgagcaa taatcgtggc ctccggacca aaggcggcgt ttccgccggt gtgatcgccg
     2401 tgccagtgag tgttgagaat aaagcgcaac ttaccctggc ccaacgtctt caacgacgcc
     2461 ctaatc
//
LOCUS       DASTEY010000228         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_21038, whole genome shotgun sequence.
ACCESSION   DASTEY010000228 DASTEY010000000
VERSION     DASTEY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21038"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFO72_04090"
     CDS             <1..836
                     /locus_tag="VFO72_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478497.1"
                     /translation="IGCTPPLTVANNFCRTIPRQGPTRLRTNSGSSIYHSLQTSLEKR
                     LTIGFSAGLHYTYSSYIDTMSEIFNVSSGEIAVAQDSFNRRADRARSSFDRPHRLAGN
                     FVYELPYFRNQRGFVGVILGGWQLNSQFSLQSGSPFTPLNGADPAGTLASISALVGNA
                     VRPNLNTTLDLSSMSVEEIVGAGGASLFRQITAAQRVGNVGRNILRSDGINNIDFGIM
                     KNTRIGENQRFQIRADFFNFTNSRDFGIPDSAVNSNNFLNQWGTDGGNRRIVVGLRYV
                     F"
     gene            856..1866
                     /locus_tag="VFO72_04095"
     CDS             856..1866
                     /locus_tag="VFO72_04095"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HET9478498.1"
                     /translation="MKPLFAILLLGTSIVCAQQTGNVEVVKASWSKVRIGWERDPFGG
                     PLENFDEMRSRARNERRIAQGGGERAKREARADEANLAKQRQKSPPRYYFIYKTKVKN
                     NHTSAITQIDWDYVFFEKDTNNEMGRQQFTSDEQIGPGKTKELTVTLTSPPTRTVSVT
                     SLNLEERDRFTEKIEVVRVQYADGRVWELSSTTPAPTPPPQSPTLGDLSWISGDWQTA
                     PGGRMQIEEHWTSVAGGSMLGMSRTVAGDKTVEFEYIRIEQRSDGIYYVAHPKARCPG
                     TDFKLTKSSATEAVFENPQHDFPKRITYRKTADEALTATIDGGEGSKAISFSFQRMKS
                     LE"
     gene            complement(2021..3379)
                     /gene="mgtE"
                     /locus_tag="VFO72_04100"
     CDS             complement(2021..3379)
                     /gene="mgtE"
                     /locus_tag="VFO72_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9478499.1"
                     /translation="MSKDLQNLLERIQLRLDESFVGIESEVADLPPADLADLLNQLRL
                     NEAAAIVSMLSLARCIELFDQPTMRRRAAIIEQLEPSRAAAILEELSADERTDVIQKM
                     GLHERHRLLPKFSQEVRAELEDLLQYPDHTAGGIMTTEFVRLDPTMAVGDALRHVRSV
                     AQEKESIYACYVMEPTTDRLLGAVSLRDLVMSELDAPVTQVMRRKPVTVNVMDDQEVV
                     AEKIGKYNLLAIPVVEQDGSVVGFVTVDDVIDVLIEEQTEDILRMAAVEPGAMDKPYF
                     HNPILRVVRNRIGWLLLLFVAGTLTSAVLHRFESELAAVVALAFFIPLLIGTGGNAGA
                     QTVMTVIRSLALGEVGVRHARRVVMREATTGMLLGLMVAPIAFVQALIWKAPFELALT
                     VSVTMFAICIWSTTVGSLVPILAQRFGVDPAVLSAPLITTLVDATGLVIYFTIAKAIL
                     GL"
     gene            complement(3386..>4379)
                     /locus_tag="VFO72_04105"
     CDS             complement(3386..>4379)
                     /locus_tag="VFO72_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET9478500.1"
                     /translation="FFGDQRLAAMREMILSDGVGAREKALEKLLPLQRGDFIAIFREM
                     AGLPVTIRLLDPPLHEFLPNAEELASELAELKMKLKGAASLSEMDHVLDEIDHKRALL
                     REVNRIREANPMLGFRGCRLGLVFPEVTKMQCRAIFEAACKVQGERNKVELEIMVPLV
                     SVSEELRQQRELIDEVARQVIGEHGMTVEYHVGTMIELPRAALMADRIATHADFFSFG
                     TNDLTQTTFGLSRDDSSRFLPNYIERKIVRDDPFQVLDRDGVGQLVRMGAERGRSQKP
                     GLKVGICGEHGGDPQSIAFCNEIGLDYVSCSPFRVPVARLAAAQAALTPVEADV"
BASE COUNT         1016 a         1306 c         1178 g          879 t
ORIGIN      
        1 tcatcggttg tactccgcct ttaaccgtgg cgaacaactt ctgtcgaaca atcccaaggc
       61 agggcccgac gcgtctgcgc accaactccg gctcgtcgat ctatcactcg cttcagacga
      121 gtttagagaa gcgcctgacc atcggattca gcgcgggact tcactacacc tacagctctt
      181 acatcgatac catgtcagag atcttcaacg tgtcgtctgg tgagattgcg gtagcgcaag
      241 actcattcaa ccgccgagcg gatcgcgcgc gttcgagttt cgatcgtccg caccgcctgg
      301 ccggcaactt tgtgtatgag ttgccttact tccgcaacca aaggggcttt gtcggtgtga
      361 tcctgggagg ctggcagctc aactctcaat tctcgctaca gagtggttcg cctttcactc
      421 cattgaatgg tgcggatcct gctggaactc ttgccagtat ttccgcgttg gtgggcaatg
      481 ccgtccggcc aaacctgaac acaacgctcg atctttcttc gatgagcgtt gaagaaatag
      541 tcggcgccgg cggagcttcg ctgttcagac agatcacggc tgcgcagcga gtgggcaatg
      601 tggggcgaaa catcctgcgc tctgacggaa tcaacaacat tgatttcggc atcatgaaga
      661 acacccggat cggtgaaaac cagcgctttc agatccgcgc agacttcttt aacttcacta
      721 actctcgaga cttcggcatt ccggactcgg cagttaattc aaacaacttc ctcaaccagt
      781 ggggaacaga cggaggcaac cgccgcatcg tcgtcggctt gcgttacgtg ttctaatcac
      841 tgaatattcg gtggcatgaa accactgttt gcgatactgc tgctgggcac atcgatcgta
      901 tgtgcccagc agacaggtaa tgtcgaggtc gtcaaggcga gctggagcaa agttaggatc
      961 ggctgggagc gcgatccgtt cggtggacca ctcgagaact tcgacgagat gcgttcacgc
     1021 gcgcgcaacg agcgccgcat tgcgcaaggc ggaggcgagc gtgcgaaacg agaagcccgc
     1081 gccgacgaag cgaacctcgc aaagcagcga caaaagagtc cgccaaggta ctacttcatc
     1141 tacaagacca aggtaaaaaa caaccacact tcagcaatca cccagatcga ttgggactac
     1201 gtcttcttcg aaaaagacac caataacgaa atgggaaggc agcagttcac cagcgacgag
     1261 cagatcggtc caggaaagac aaaagagtta actgttaccc ttacgtcgcc gcctactcga
     1321 actgtgagtg tcacttcact caacctcgaa gaacgagatc gtttcactga aaagattgaa
     1381 gtagttcgtg tgcagtacgc cgatggccgc gtgtgggagc tttcatccac tacgccggcg
     1441 ccaacaccac ctccgcaatc gccgacactg ggggatctct cgtggatctc gggtgactgg
     1501 cagacggcgc ccggtggacg catgcagatc gaagagcatt ggaccagtgt tgctgggggg
     1561 tcgatgctgg ggatgagccg aacggtcgcg ggtgataaga cggttgagtt tgaatacatt
     1621 cggatcgagc aacgctctga tggcatctat tacgtggcgc atccgaaagc ccgctgccct
     1681 ggcaccgatt ttaagttgac caaatcctcg gctactgaag ctgttttcga aaacccgcag
     1741 cacgactttc caaaacggat cacttatcgc aaaaccgctg acgaggcgtt gacggcaacc
     1801 atcgacggcg gcgaaggcag caaagccatc tccttctcgt tccagcggat gaagtctctc
     1861 gaatgaagga acctgtttcc tgggaccgca ggcgtctcgc ctgctgtgca gcgaagaagg
     1921 ccactgccga atccagcagg cgagacgcct gcggtcccag ggaagagcac gaccgaggga
     1981 agagcctacc ggggtggaag tggcgcttat cccccagcga ctagaggcct aagattgctt
     2041 tggcgatcgt gaagtagatg accagcccgg tggcatcgac caaggttgta atcagaggcg
     2101 ccgacagcac tgccgggtca actccaaaac gttgggccaa aatcggaacc aacgatccca
     2161 ccgtcgttga ccagatgcag atcgcaaaca tcgttaccga aacagttagc gccagctcga
     2221 aaggtgcttt ccagatcaac gcctgtacga acgcgattgg agcaaccatc aagccaagaa
     2281 gcattccggt tgtcgcttcg cgcatcacca cgcgccgcgc gtgccgcacg ccaacctcgc
     2341 ccagcgccaa actccgaatc actgtcatca ccgtttgcgc acccgcattg ccgccggtac
     2401 cgatcagcaa cggaatgaaa aacgcgagtg caacaaccgc ggccagttca ctctcaaacc
     2461 gatgcaacac cgcgctagtg agcgtgccgg ccacaaacag caacagcagc caaccgatcc
     2521 ggttacgcac gacgcgcaag atcgggttgt ggaaataagg cttgtccatc gcacccggtt
     2581 ccacggccgc catgcgcagg atgtcttccg tctgctcttc aatcagcacg tcaatcacgt
     2641 cgtcgactgt gacgaagccg accacgctgc cgtcctgttc gactaccggt atggcaagca
     2701 agttgtactt tccgatcttc tcggccacta cctcctgatc gtccatcacg tttaccgtga
     2761 ccggtttgcg tcgcatcact tgtgtaacgg gagcatcgag ttcggacatg acgagatccc
     2821 gcaacgacac agctcccagc agccgatcag tcgttggttc catcacgtag cacgcgtaaa
     2881 tcgattcttt ctcctgtgcc acagaacgaa cgtgacgcag cgcgtcgccc acggccatcg
     2941 tgggatcgag ccgcacaaac tcggtcgtca taatgccgcc ggccgtatga tccggatact
     3001 gcagaaggtc ttccagctct gcacgcactt cctgactgaa cttcggcaac aaacgatgcc
     3061 gttcgtgcag acccattttc tgaatgacgt cagtgcgttc gtcggcggag agttcctcga
     3121 gaatggcagc ggctcgcgag ggttcgagtt gttcgatgat ggctgcgcgg cgccgcatgg
     3181 tgggctgatc gaaaagttcg atgcagcgcg caagcgacag catcgagact attgccgccg
     3241 cttcgttgag acggagttgg ttgagcaagt cggcgagatc tgctggcgga aggtccgcga
     3301 cttccgattc gataccgaca aagctttcgt ccagccgtag ctgaatgcgt tcgagaaggt
     3361 tctgtagatc cttcgacacg agatctcaga cgtcggcttc aaccggagtc aacgcagcct
     3421 gggccgcggc caaacgcgca acgggaacgc ggaacggcga acaacttaca tagtcgagac
     3481 cgatctcatt gcagaacgcg atcgactgcg gatcgccacc gtgctcgccg cagatcccca
     3541 ctttcaagcc gggcttttga ctgcgtccac gttcggcacc catccggaca agttggccaa
     3601 caccatcgcg atcgagaacc tgaaacggat cgtctcgcac gatctttcgt tcgatgtaat
     3661 ttgggaggaa gcggctggag tcgtcgcggc tcaacccaaa cgtcgtttgc gtcaggtcgt
     3721 tggtgccaaa cgaaaagaag tcggcatgcg tcgcgatacg atcggccatc agcgcggcac
     3781 gcggcaactc gatcatcgtt ccgacgtggt attcgacagt catcccgtgc tcacctatga
     3841 cctggcgcgc aacttcgtcg atcagttctc gctgctgccg caactcttcg cttacagaaa
     3901 ccagcggcac catgatctcg agctccactt tattgcgctc gccctgtacc ttacaggcgg
     3961 cctcgaagat cgcgcgacac tgcattttcg tcacttccgg aaatacaaga cccaaacggc
     4021 aaccgcggaa cccgagcatc ggattggcct cacgaattcg attcacttct cgcagcagcg
     4081 cccgtttgtg gtcgatttcg tcgagcacgt ggtccatctc actcaaagac gcagcgcctt
     4141 tgagtttcat cttcaactca gccagctcac tcgcaagttc ttccgcattc ggcaaaaact
     4201 catgcagcgg cggatccaac agacggatgg ttacaggcag cccggccatt tcgcgaaaga
     4261 ttgcgatgaa gtcaccacgt tgcaacggca gtaacttctc caacgccttc tcccgtgcgc
     4321 caacgccgtc cgacaggatc atctcgcgca tggctgcgag ccgctgatcg ccaaaaaac
//
LOCUS       DASTEY010000229         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_516028, whole genome shotgun sequence.
ACCESSION   DASTEY010000229 DASTEY010000000
VERSION     DASTEY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516028"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..858
                     /locus_tag="VFO72_04110"
     CDS             166..858
                     /locus_tag="VFO72_04110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9478501.1"
                     /translation="MGLKNFVASYSSRHYDALRSIFGESLLGRLGVRRYWYEIDGWFH
                     WRSAQEEAVSSFPDGSRFVEVGTYLGRSLCSLGEVVERSGKNIGVIGIDTCRGNGPEG
                     WRSQDYHAGAVQNGGGTFAGTLHKNVLDCGFGDKIVLIISDSVSASRLFGDASLDWVH
                     LDARHDYASVKTDIEAWLPKVKPGGWLSGDDYDTQKWPEVVKAVGDVLPRAKPWSNQQ
                     WRWIVGNADNAD"
     gene            complement(888..1517)
                     /locus_tag="VFO72_04115"
     CDS             complement(888..1517)
                     /locus_tag="VFO72_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478502.1"
                     /translation="MPWIVLTDEPDDFAGLPVRAIRHAPTGPMAIDFLTKLPVFGNGR
                     GQPAYHDKRFALEAALKEFDTAVFLDADTRFSSVPKLPRLPAGISVTRAVKASIADHL
                     SRWGTHRKPVFEELAVHLTGTTETLKSARWCSEALFAITKDGNESRFFEAWARAAEFL
                     QSKEVFSGEGGTIGLAAACAGWTVNYDSLNKLAASTQHEGRGPKSGAAD"
     gene            complement(1671..>2578)
                     /locus_tag="VFO72_04120"
     CDS             complement(1671..>2578)
                     /locus_tag="VFO72_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9478503.1"
                     /translation="IRKGEIPGPTLYVSGPFIQHEPYPGTDYVRWGVSGADDARAKVR
                     TLAEAGVDVIKLIDQDQMTMDEVRAVVDEAHKRKLPVVGHSHRPEEIRRGLEVGVDCF
                     EHTGLATAPEYPAEIIAMIRERTAKMSLGPLFWTPTVQGLLNYEYVRDNPEQLDDPSW
                     QVGLPQSIIEDIKGSLKKPGQLGYYQITPLRRPTLARKISQLSESGVVLLIGTDSGIP
                     MNFHSQTTWRELDAWVNAFGINPMTAIRAATYWPSVAMKVSDQVGTITEGKYADIIAV
                     RGDVLRHINLLQNVDIVIKHGRRWK"
BASE COUNT          537 a          706 c          744 g          591 t
ORIGIN      
        1 ttgaggtatg aaaagcacaa ccgtgaagtg ctcgagtatt ttggcgacag agtgggtaaa
       61 gatttattgg tggtgtcgtg ggaaaaagga gatggttggg cggagttgag ccgtttcctc
      121 aacaagcgcg cgcccgacga accgtttccg catttgcgat aagcaatggg actcaagaac
      181 ttcgtagcga gttactcgtc gcgccactac gacgcgctgc gatcgatctt tggcgagtcg
      241 ctgcttgggc gcttgggtgt ccgtcgttac tggtatgaga tcgatggctg gtttcactgg
      301 cgttcggcgc aagaggaggc agtgagctcg tttcctgacg gtagccgctt tgttgaagtt
      361 ggaacgtatt tggggcgtag tctttgctcg ctcggtgaag tggttgagcg cagtggtaag
      421 aatatcggcg tgatcggaat cgacacttgt cgtggcaatg gtcccgaggg ttggcgtagc
      481 caggattatc acgccggtgc ggtccagaat ggtggtggta catttgccgg cacgttgcac
      541 aagaacgtat tggattgcgg gttcggagac aagatcgtgt tgataatttc ggactccgtc
      601 agtgcctcgc gattattcgg cgacgcatca ctcgactggg tccacctgga cgcgcgacac
      661 gactacgcga gtgtcaaaac cgacatcgag gcgtggctcc cgaaagttaa accgggcggc
      721 tggttatccg gcgacgatta cgacacgcaa aagtggccgg aggttgtgaa agctgtgggc
      781 gacgtcttac cccgcgcgaa accctggtcc aatcagcaat ggcggtggat tgtaggtaac
      841 gcagataacg cggattagat ccggatttta tccgtaaacc atccgtgtta atccgcggcc
      901 ccggattttg gtccgcgtcc ttcgtgttgc gtcgaagctg cgagtttgtt gaggctgtcg
      961 taattcacgg tccagcccgc gcacgccgca gccagcccga tggttccgcc ttcacctgaa
     1021 aacacttcct tgctttgcag gaactcagcc gcgcgcgccc aagcctcgaa gaacctcgac
     1081 tcattcccat ccttcgtaat ggcaaacaac gcttcgctac accaacgcgc cgacttaagc
     1141 gtttcggtcg tacccgtgag atgtacagcc agttcttcga aaaccggctt acggtgcgtc
     1201 ccccaacgac tcaaatggtc cgcaatgctt gctttcaccg ctcgagtaac cgaaatgcca
     1261 gccggcagtc gcggcagttt gggcaccgag ctaaaacgcg tatccgcgtc aaggaaaacg
     1321 gcggtatcaa actctttcaa ggccgcctcc agggcaaatc gcttatcgtg ataggctggt
     1381 tgccctcttc catttccaaa caccggtaac tttgtcaaaa aatctatcgc cattggacca
     1441 gtgggcgcat gacgaatcgc gcgtactggc aatccggcaa agtcatccgg ctcatcagtc
     1501 aacacaatcc acggcacctc cggtacatcc ctgacgagta gccttgccct ccgccggtac
     1561 ggcgcatgaa tcgcgagcgt gcaaaagcaa acatttgccg cacgagggcg ccgccgattc
     1621 acgagagctt taagcatgaa tcttttgtgc tttttgtgcc tttttgtggc tcacttccat
     1681 cttcgcccgt gcttgatcac gatatcgacg ttttgcagga gattgatatg ccgcagcacg
     1741 tcgccgcgga cggcgatgat gtcggcgtac ttgccttcgg taatggttcc gacttgatcg
     1801 gagactttca tggcgactga gggccagtag gtggcggcgc ggatggcggt catggggttg
     1861 atgccgaagg cgttgaccca ggcgtcgagt tcgcgccatg tggtttggct gtggaaattc
     1921 atggggatgc cgctgtcggt gccaatcaag agcaccacgc ctgattcaga gagttgggag
     1981 atcttgcgcg cgagtgtcgg acgacgcagc ggcgtgatct ggtagtaacc gagctgtccc
     2041 ggtttcttca gagagccttt gatgtcctcg ataatcgatt gcggcagtcc aacttgccac
     2101 gatggatcgt caagctgctc gggattgtca cgaacgtact cgtagttcaa aagcccctgc
     2161 acggtcggcg tccagaacaa cggccccaga ctcatcttcg ccgttcgttc gcgaatcatc
     2221 gcgatgatct ccgccggata ctcaggcgcg gtcgcgagtc ccgtgtgttc aaaacagtca
     2281 acaccaactt cgagcccgcg acgaatctct tccggtcgat gtgaatgacc gacgaccgga
     2341 agcttgcgtt tgtgcgcctc gtcgacaact gctcgcacct cgtccatcgt catctgatcc
     2401 tgatcgatca gcttgatcac atcgacgcct gcttcagcca acgtgcgcac tttcgcgcgc
     2461 gcatcgtcgg cgccgctaac accccagcga acgtaatcag tgccgggata aggttcgtgc
     2521 tggataaaag gtcctgacac gtaaagagtt ggaccaggta tctcgccttt acgaatgc
//
LOCUS       DASTEY010000230         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1010366, whole genome shotgun sequence.
ACCESSION   DASTEY010000230 DASTEY010000000
VERSION     DASTEY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010366"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1192
                     /locus_tag="VFO72_04125"
     CDS             305..1192
                     /locus_tag="VFO72_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478504.1"
                     /translation="MPSSTGYLTAAIIVAAALFFLLRWMLVRSGDEAPWLPAGLAASV
                     VLLVALSAREVVMRRAWTRYLLENGIHDARPKRRSAQTTRSSKKGLSASVHSAALRAI
                     QKQSATADSPHSPPEAHLDVAQLCQEYLASTDEAIRSGSYGSEKGIAIRAGQERVRSL
                     HKHHLLTWARGQSRLLTREAQQRARTFDKIETANRALDCIDSALRIYPEEPELHESKA
                     AIGEFIASVKVAHWVELAERSAFKGHYRRAVDRYRDALFYLNQDVVKAEVRTAGTEKI
                     EREIADLQAKLRQRKNQDQ"
     gene            1396..>2259
                     /locus_tag="VFO72_04130"
     CDS             1396..>2259
                     /locus_tag="VFO72_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9478505.1"
                     /translation="MRLKQCLIVAIACACMLAGFSHAQEPQQQQQTSAPSGSESPRLD
                     IQGSKNYLLGPGDVLDIRVFGQPDLSTPAAQIDGDGNLSSLPFLETPIPAKCRTDKQV
                     QKDIAVAYSKLIKNPQVSVRIAERNSRQPATVFGAVRQSSRIEMKRKVRLNELIAVSG
                     GFTERASGTIQILHTEPLMCPEPGEEEEAKPIDGTRIPLQIVKISELRAGKAEANPVI
                     RPGDYVLVTEAEPVYVTGAVVSPSGIYLRDQLTLTRALAMVGGARKEAKLSEVMIYRQ
                     TPGSTKQEPPIK"
BASE COUNT          511 a          638 c          660 g          450 t
ORIGIN      
        1 gctcttccct gggaccgcag gcgtctcgcc tgcaaggcga caaattgggc gctctcgtga
       61 caatgcaggc gagacgcctg cggtcccagg gagaacacgt cacagaatcg gcgcccggtt
      121 caaactgcag caactaccgg gcacatgcgc ggacaaaaca tagtttaggg tttagtaccc
      181 ggttggttaa ccaacccgga ggcggttgtt gggaaacaac tcagcagatc agtttttccc
      241 tcgcaatccg cgaacacatt cgcgattgca cgacgaggtg cgccaccgtc cggcacgagg
      301 ccgaatgcct tccagcacgg gatacctgac ggctgccatc attgtggctg ccgcgttgtt
      361 ctttttgctg cgctggatgt tggtgcgtag cggcgatgaa gcgccgtggt taccggctgg
      421 tttggctgcc agcgtcgtgt tgctggtcgc gttgtcggcg cgggaagttg tgatgcgtcg
      481 cgcctggacg cgttatctgt tagagaacgg cattcacgac gcgcggccga agcgtcgctc
      541 cgcgcagacg acgcgcagca gcaaaaaagg tctttcggcc tctgtgcatt cggctgcatt
      601 acgcgccatt cagaaacaat cggccaccgc cgattcgcct cactctcctc cggaagcaca
      661 tctcgacgtg gcccaacttt gccaggaata tctggcaagc actgacgaag cgattcgctc
      721 tgggagctac ggcagtgaaa agggaattgc cattcgcgcc ggccaggagc gcgttcgctc
      781 actgcataaa catcacttgc tgacttgggc caggggacag tcacgcttat taacccgcga
      841 agcacagcaa cgcgcccgca cgttcgacaa gatcgaaacg gcgaatcgtg cactcgactg
      901 catcgactca gcgttaagaa tctatcccga agaaccggaa ctgcacgagt cgaaagccgc
      961 gatcggtgag ttcattgcct ccgtcaaggt tgctcattgg gtggaactgg ccgaacgctc
     1021 ggcgttcaag ggccattacc ggcgtgcggt cgaccgctat cgcgacgcac tcttttatct
     1081 gaaccaggac gtggtcaagg cagaggtgcg cacggccgga acagagaaga ttgaacggga
     1141 aattgccgat ctgcaggcaa aactgcggca acgcaaaaac caggaccaat gacttttcag
     1201 aagtgtccga agtgtggcag ctcaaggatt cgcagaggtt ataaacggac tcctttgccg
     1261 ttgcgtctgg ttggcatcta ccatcttttg tgcgatcatt gcaacttgct ttttactggc
     1321 tttgtgttgc cggggacctt cagccgacga agaaaacatc ggcggcagag aaaaacttca
     1381 cagtgaggta atgtcatgag attaaagcag tgtttgattg ttgccattgc ttgtgcgtgc
     1441 atgcttgccg gttttagcca cgcgcaggaa cctcaacaac aacaacaaac gtccgctccc
     1501 agcggttctg aatcgccgcg tctggacatt cagggaagca agaactatct gcttggacca
     1561 ggcgacgtgc tcgatatccg cgtgttcggg cagccggact tgtccactcc cgctgcgcag
     1621 atcgacggcg acggtaacct gagctcgttg ccattcctgg aaactcccat tccggcgaaa
     1681 tgccgcacgg acaaacaagt gcagaaagac attgccgttg cttactcgaa gctcatcaag
     1741 aacccgcagg tcagcgtcag aatcgcagag cgcaacagcc gtcagccggc gacggtattt
     1801 ggcgcagtgc ggcagtcgtc acggattgag atgaaacgga aagtgcgttt gaatgaactg
     1861 atcgcggtgt cgggcggctt taccgaacgc gcctccggca cgattcagat tcttcacacc
     1921 gaaccgttga tgtgcccgga acccggcgaa gaggaagaag cgaagccgat tgatgggacc
     1981 aggattccac ttcagatcgt gaagatctcc gaactgcgcg cgggaaaggc cgaggcgaat
     2041 ccggtcatcc gtcccggcga ctacgtgctg gtgaccgaag cggagccggt ttacgttact
     2101 ggcgcagtcg tctcgccgag cggcatctac ctccgcgacc agctcaccct aacgcgcgct
     2161 ctggcgatgg tcggcggcgc gagaaaagaa gctaagttga gcgaggtcat gatctatcgt
     2221 cagactccgg gttccacgaa acaggaacca ccaatcaag
//
LOCUS       DASTEY010000231         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1241073, whole genome shotgun sequence.
ACCESSION   DASTEY010000231 DASTEY010000000
VERSION     DASTEY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241073"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..2130)
                     /locus_tag="VFO72_04135"
     CDS             complement(82..2130)
                     /locus_tag="VFO72_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9478506.1"
                     /translation="MKPIFLSVMTVLAGLTLALAVQAQQPAPQQAASRFDVTNYRIEA
                     QIIPDEHMLRAGADVTFVPLDATRSVVFELNGSLKVESIEMAGKALTGFVQDAVGVGS
                     LGPSVRIDLGQVIPANQPVTLRIRWSGALVSPEGGPLATRRLAYVGNEGSYLMYASRW
                     FPFHDYAADRATSDITFIVPSGLQVAGTSDDPVVAQPSPKEGAVRYRFVHRQPVLIGN
                     FAAGQYINRNLRYSNYDLQLYAMPGSEKRLEAFAELTGRVLEFYTKQYGKPLFGNRLV
                     VVQIDDESLDTYSGPGIIFLSSKMFDTSRPVPEEKIQREIAYQWWGQTVGLKSFDDAW
                     LSQGLAEWSAFAFRETTLSAGGLDGAQREQQERALTFEQTASIARAPSALDDQSAAYQ
                     SIVYYKGSMVFRMLRETMGRENFDRLLKTFLDTYRAKNASIDDFEKLAAKVSQQNLRY
                     FFAQWVEGTGVPEFTVDYQIIRTRSGKFRTRGTVKQNLEALHMPVELLLRAEGDNQQK
                     IVRIETRSEDFDFESNGQPLEVVVDPNNKILRMSEDLRVSIIARRGIEQMKEGIYAEA
                     QQQFEAALKLDRSNSWVYYNLGLLFLEQRNWQQALDNLEASLIGNLKPAWIEVWAHIK
                     RGNAYDAKGERQRAVLEYNKAVQSGIDYDNAQAVAKKYLATPYDPKAGQTAEATTPGN
                     "
     gene            2370..>3439
                     /locus_tag="VFO72_04140"
     CDS             2370..>3439
                     /locus_tag="VFO72_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9478507.1"
                     /translation="MRRVSKFLSPLVVCALAVLSAQAQAPRNVRVDFKETTLKNGLRV
                     ITVEDHSAPVVSLSITYNVGSRDERKGRTGFAHLFEHMMFKGSENIGSGEHYMLVFNN
                     GGNMNGTTNEDRTNYFEVLPANQLDLALFLEADRMKSLAITKENLDNQRNAVQEERRL
                     GVDNQPYGKSSERQQELLYDNFAYKHSVIGSMEDLNAATVEDVAAFFKMYYAPNNAVL
                     VLVGDVNTTEALAKIRSSFESIPRQPNPPAVDMTEPQQKAERRDSLNDMLARAPRVDL
                     AYKAVAGNTPDFYALQVLGAVLQSGQSSRLYQKLVKEKEMVTSVGGFMDEKRGIGAFY
                     SNATLRPGVKTEDVEATIYAEI"
BASE COUNT          771 a          988 c          934 g          746 t
ORIGIN      
        1 tcaatcgtag ccacggatta acacggattt cattcacgga ttttattcag atctgtgtat
       61 gaaatccgtg tttatccgtg gctaatttcc gggtgttgtc gcttcggcgg tttggccggc
      121 tttcggatcg tagggtgtcg ccaggtactt tttcgctacc gcctgcgcat tgtcgtaatc
      181 aattcccgac tgcaccgcct tgttgtactc caggaccgcg cgttggcgtt cgcccttcgc
      241 atcgtaagca ttgccccgct tgatgtgggc ccacacttca atccaggccg gtttcaaatt
      301 cccgatcagc gaagcttcga gattgtcgag cgcctgctgc cagttgcgct gctcgagaaa
      361 cagcaaaccg agattgtaat aaacccacga attggaacga tcgagtttca acgccgcttc
      421 aaactgctgc tgcgcctcgg cgtaaatgcc ttccttcatc tgctcgattc cgcgtcgcgc
      481 gatgatcgaa acacgcaggt cttctgacat gcgcaagatc ttgttgttcg ggtcgactac
      541 gacctcgagc ggttgtccgt tcgactcgaa atcgaaatcc tcgctgcgtg tttcaatccg
      601 aacgatcttc tgctggttgt cgccttcggc acgtaacagc agttcgaccg gcatgtgcag
      661 tgcctcgagg ttctgtttga ccgtgccgcg cgtgcggaac ttgccggagc gcgtgcgaat
      721 gatttgatag tcgacggtaa actccggaac acctgttcct tcaacccact gcgcaaagaa
      781 gtaacgcagg ttttgttgcg agactttcgc cgctagtttt tcgaagtcgt cgatcgacgc
      841 attcttagct cgataggtat cgaggaaagt tttcagcagc cgatcgaagt tttcacggcc
      901 catcgtctcg cgcagcatgc gaaagaccat cgaacccttg taatagacga tcgactggta
      961 agccgcggat tgatcgtcca gagcgctcgg cgcgcgcgca atcgatgcgg tttgttcgaa
     1021 cgtcagcgcg cgttcctgtt gctcgcgttg agcgccgtcg agaccgcccg cgctcaaggt
     1081 cgtctcgcga aacgcgaacg cactccactc ggcgagacct tgcgatagcc acgcatcgtc
     1141 aaacgatttc agaccgactg tctggcccca ccactggtaa gcaatctctc gctgaatctt
     1201 ctcttcgggc acgggacgcg acgtgtcgaa catcttgctc gacaggaaga tgattcccgg
     1261 acccgaataa gtgtctaaac tctcatcgtc gatttgaact acgacgagcc ggttaccaaa
     1321 gagtggttta ccgtattgtt tggtgtagaa ctcaagcacg cggcccgtca gttcagcgaa
     1381 ggcttcgagc cgtttttcac tgcccggcat tgcgtaaagc tgaaggtcgt agttgctgta
     1441 acgcaggttt ctgttgatgt actggcctgc cgcgaagttt ccgatcagca ctggctggcg
     1501 gtgaacaaat cgatagcgaa cggcgccttc tttcggcgac ggttgcgcga cgactggatc
     1561 gtcgctggta ccggcgacct gcaatcctga aggcacgata aacgtgatgt cggaggtcgc
     1621 tctgtcagcg gcgtaatcat gaaaaggaaa ccagcgcgag gcatacatca ggtaggaacc
     1681 ttcgttaccc acgtaagcca atcgccgggt tgccagcggc ccaccttccg gagaaacgag
     1741 cgctccgctc cagcgaatgc gcagcgtcac cggctggttc gcgggaatca cctgaccaag
     1801 gtcgatacga acgctagggc cgagggagcc gacgccaacg gcgtcctgaa cgaatccggt
     1861 caaggccttg ccggccatct cgatgctctc aacttttagc gagccattga gttcaaaaac
     1921 gaccgaacgg gttgcatcca gcggcacaaa cgtcacgtcg gcgccggcgc ggagcatgtg
     1981 ctcatcgggg atgatttggg cttcgatccg gtagttcgtc acatcgaagc gactggcagc
     2041 ttgctgaggc gccggttgct gagcttgcac tgcgagggcc aaagtcagcc cggccaaaac
     2101 agtcataaca ctgaggaaga taggcttcat tcttttcatg gcattattgc gacaattata
     2161 cagtcgaggg gtttggatgg taagaagtca ggggcggttg gctgagtcag ggccgcagat
     2221 ttaggccgat taacgcaaat ttgcgtcaac ccgcggaaat ctgcggctcg gcttgacact
     2281 acactctcca gcgcatacag tcgctgccac acggtagtag tccagaacct tcgaccgagt
     2341 catcatccac atctaaagga gtttgagtaa tgagacgcgt ctctaagttt ttgtccccac
     2401 tcgttgtctg cgctctcgca gtgctctccg cccaggcaca ggcacctcgc aacgtgcgag
     2461 ttgatttcaa ggaaaccact ttaaagaacg gtctgcgcgt gatcacagtt gaagatcatt
     2521 cagcgcccgt agtatcactc tccatcactt acaacgtcgg ttcgcgcgac gaacgcaagg
     2581 gtcgcaccgg gttcgcgcat ctcttcgaac acatgatgtt caagggctca gagaatattg
     2641 gatccggtga gcactacatg ctcgtcttca acaacggcgg caacatgaat ggcaccacca
     2701 acgaagatcg cactaactac ttcgaagtgc tgcccgccaa ccaactcgat ctggctctct
     2761 tcctcgaagc cgatcgcatg aagagtttgg ccatcaccaa ggaaaatctc gacaaccaac
     2821 gcaacgctgt ccaggaagaa cgcaggcttg gcgtcgacaa ccagccgtac ggcaagagca
     2881 gcgaacgcca gcaggaactg ctctacgaca acttcgctta caagcattca gtgatcggtt
     2941 cgatggagga cctgaacgca gcgactgtcg aggatgttgc cgcgttcttc aagatgtact
     3001 acgcaccgaa caacgccgtg ctcgttctgg tcggcgacgt caacaccacg gaagcgctgg
     3061 ccaagatccg cagcagcttt gaaagcattc cacgccagcc caatccgccc gcggtcgaca
     3121 tgactgagcc gcagcagaaa gccgaacgac gagactcgct caacgacatg ctcgcacgcg
     3181 cgccgcgcgt cgatctcgca tacaaagcag ttgcgggcaa cacgcctgac ttttacgcgc
     3241 tgcaagtgct cggagcggtg ctgcaaagcg gccagagctc gcgtctctac cagaagctcg
     3301 tcaaagagaa agagatggtt accagcgtgg gcggtttcat ggatgagaag cgcggcatcg
     3361 gcgcctttta cagcaacgcc actcttcgac caggcgtgaa gaccgaagac gtcgaagcaa
     3421 ctatctacgc cgagatcga
//
LOCUS       DASTEY010000232         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1158617, whole genome shotgun sequence.
ACCESSION   DASTEY010000232 DASTEY010000000
VERSION     DASTEY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158617"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFO72_04145"
     CDS             complement(<1..369)
                     /locus_tag="VFO72_04145"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HET9478508.1"
                     /translation="MSTEASQRGSGPLTERGTGRLRTPQAEPLSDATSQTPAGFMRRM
                     TPAEFNAAMIHFYRGEVQRSNTWRNRLDTTTNWAVITAGATLSFVFSSPNNPHFVIPI
                     NSILVVIFLLMEARRYRYYEI"
     gene            555..1034
                     /gene="tadA"
                     /locus_tag="VFO72_04150"
     CDS             555..1034
                     /gene="tadA"
                     /locus_tag="VFO72_04150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET9478509.1"
                     /translation="MSAAELDFDEQWMRQALAAAREAQANGEVPIGTCIVSENKLVAV
                     SGNRTRTDCDPTAHAEIVALREAARKLGNYRLTGVDVYSTIEPCAMCAGALIQARVRR
                     LVYGAPDERAGAVASRFRICDTDFLNHRIEVSAGVLEAECRALMQEFFQTRRGRIVD"
     gene            1012..1101
                     /locus_tag="VFO72_04155"
     tRNA            1012..1101
                     /locus_tag="VFO72_04155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1046..1048,aa:Ser,seq:cga)
     gene            1158..1679
                     /locus_tag="VFO72_04160"
     CDS             1158..1679
                     /locus_tag="VFO72_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET9478510.1"
                     /translation="MSDEKLAIDEARRAAQHETVKSQVEGEVQSEIGERAATKGTPGE
                     AQRIDQVAGEFRSKAVNEVVDTEREVERGRGLARVSQVVDYIFWVIYALLGMRFLLAL
                     LAARSSAGFVQFIVTVSNPFYAPFRGIVASPSTDAGHTLLLPVVVAIISYALLHLGIN
                     ALLRMIAHRKTEI"
     gene            1688..1786
                     /gene="ffs"
                     /locus_tag="VFO72_04165"
     ncRNA           1688..1786
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFO72_04165"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1871..2608
                     /locus_tag="VFO72_04170"
     CDS             1871..2608
                     /locus_tag="VFO72_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478511.1"
                     /translation="MKKIYLATAFIFTLVLSAFSVSAQTQSRDDVIKQIEAKRAELAV
                     LEQKVLAVADSDREEFAAFLSQPQTGIIRLLPRETYDGNAKRALAIRGGGAYYSFVRK
                     THEYGYGSDIELAQEQLSAGFAGADYGMLLNLGDVPLEQVANDHAATRALLDYTPPVK
                     EPEVRAVQRKLWQGIELSGITFKDRILAKVSNTYLLRSISVDRSDILVVFRVVRQDTD
                     GSLIIVYRVLKTFPKPTMERTPITEGN"
     gene            2622..>2861
                     /locus_tag="VFO72_04175"
     CDS             2622..>2861
                     /locus_tag="VFO72_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478512.1"
                     /translation="MAYQVIARKWRPQTFDEVTGQEPITRTLRNAIEHRRLHHAYLFS
                     GARGVGKTTSARLLAKALNCHKSSEPTPTPCRADDP"
BASE COUNT          626 a          769 c          847 g          619 t
ORIGIN      
        1 gatctcgtaa tagcgatagc ggcgcgcttc catcaaaagg aaaatcacga ccagtatcga
       61 gttgatcgga atgacgaaat gcggattgtt cggcgagctg aacacgaacg acaacgttgc
      121 gcccgcggtg atgacggccc agttcgtagt tgtatcgagt cgattacgcc aggtgtttga
      181 gcgttgcact tcgccgcggt agaagtggat catcgcggcg ttgaactccg ctggcgtcat
      241 ccggcgcatg aatcccgcgg gagtttggga agtagcgtca gacaaaggct ctgcctgcgg
      301 agttctgagt cgccccgtgc ctctttctgt taatggaccg ctacctcttt gacttgcttc
      361 ggtggacatc ggaattgaat cggtggagcg cgccggccag agcggggcgc ggaactccgg
      421 aaattgataa taacacgcgc gattgtcttt ggttagttga gatttatgaa ccggtgtctt
      481 tgcgcctttg cgtgaaacct tctttttgcc atatattggc cgctcggttg tttgaaggac
      541 gtagatcgct ggtgatgagc gccgcggaat tagactttga cgagcaatgg atgcggcaag
      601 cgctggccgc cgctcgcgaa gcgcaggcaa acggcgaggt gcccatcggc acttgcatcg
      661 ttagcgaaaa caaactcgtc gctgtttccg gaaatcgaac gcgtaccgac tgcgatccca
      721 cagcccacgc tgaaatcgtt gctcttcgcg aggccgccag gaagctggga aactaccggc
      781 tgacgggcgt ggatgtttac tcgacgatcg agccgtgcgc gatgtgcgcc ggggctttga
      841 tacaggcccg ggtgcggcgt ttagtttacg gcgcgccgga tgagcgggca ggtgcggtgg
      901 cgagtcgctt tcgaatctgc gacacggatt tcctgaatca ccggatcgag gtcagcgcgg
      961 gcgtattgga agcggaatgc cgcgcgctga tgcaggagtt tttccaaacg cggagaggtc
     1021 gcatagtgga ttagtgcacc ggtctcgaaa accggagtgg gtttacgccc accgtgggtt
     1081 cgaatcccac cctctccgcc agatatcgtt tccccgacgc ccccacaata cacacactct
     1141 gttggttggg gtatgcgatg agcgacgaaa agttggcaat tgacgaagca agacgtgcgg
     1201 cacaacacga gacggtaaag agtcaggttg agggcgaagt tcagtccgaa atcggcgaac
     1261 gtgccgcaac aaaaggaacg ccgggcgaag cgcagcgtat tgatcaagtc gccggtgaat
     1321 tccgatcgaa ggctgttaac gaggtcgttg acactgagcg cgaagtcgaa cgcggccgcg
     1381 ggctggcacg agtttcccag gttgtcgatt acatcttctg ggtaatctac gcgcttctcg
     1441 ggatgcggtt cctgctggcc ttgctggcgg cgcgcagcag cgccgggttc gtgcagttta
     1501 ttgtgacggt cagcaatccc ttttacgctc cttttcgggg gattgtcgcc agtccaagca
     1561 ccgatgccgg tcacacattg ctcctgccgg tcgtagtagc gataatctcg tatgcgctgc
     1621 ttcatctcgg catcaatgca ctgttgcgga tgatcgcgca tcgaaagacg gagatttaga
     1681 tatcagatcc cgggctccgc acaacgctag ccttgtgaac cccgccaggc cgggaacgga
     1741 gcaacggtag caacggcagc gtgtgttgcg ggaaagtccg gggtctggcc agatcagctt
     1801 tacaacgcgt agtagtctca caactaaccc tctcccttgg ctttgctcaa acagaggagg
     1861 ttgagtcccg atgaaaaaga tctatttagc tacagctttc atcttcacgc ttgtcctaag
     1921 cgcgttttcc gtctcggcgc aaacgcaatc tcgcgatgac gtcatcaagc agatcgaagc
     1981 aaagcgcgct gaactcgccg tgctggagca gaaagtcctt gctgttgccg actcagaccg
     2041 cgaggagttt gctgcctttt tgagtcagcc tcagaccgga attatccggt tgttaccccg
     2101 cgagacatac gacggaaacg ccaagagagc cctcgcaatc cgcggtggtg gcgcttatta
     2161 ctcgttcgtt cgtaagactc atgaatacgg gtacggctcg gacattgaac tggcacagga
     2221 gcagctatcg gccggttttg cgggcgctga ttacggaatg cttttgaacc tcggggacgt
     2281 tccgctcgag caagtggcga acgatcatgc tgcgacgcgc gcattgttgg actatacgcc
     2341 gccggtaaaa gagcctgagg tccgtgccgt gcagcgcaag ctctggcagg gtatcgagct
     2401 ttcggggata accttcaagg accgcatact ggctaaagtc tcaaacacgt atctgttgcg
     2461 atcgatctcg gttgacagat ctgacatctt ggtcgtgttt cgcgtcgtcc gacaagacac
     2521 cgatggaagt cttatcatcg tttacagggt gctgaaaact tttccaaaac cgacaatgga
     2581 aagaactccg atcacagaag gaaactagtc tctacacatc gatggcctat caagtaatcg
     2641 ctcgcaagtg gcgaccgcag acgtttgacg aagtaacggg tcaggaacca atcacacgca
     2701 cgctgcgcaa cgccatcgag catcgcaggc tgcatcatgc gtacctgttc tcaggcgcgc
     2761 gcggcgtagg taaaactacc agcgcgcgct tgcttgcgaa ggcgctcaac tgtcacaagt
     2821 catccgaacc aacgcccacg ccctgccggg ctgacgatcc a
//
LOCUS       DASTEY010000233         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1570416, whole genome shotgun sequence.
ACCESSION   DASTEY010000233 DASTEY010000000
VERSION     DASTEY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570416"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="VFO72_04180"
     CDS             <1..2157
                     /locus_tag="VFO72_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9478513.1"
                     /translation="AERDDEHYRQQVAIKLIRAGLSGEAIRKRFRNEMQILADLNHPN
                     IARLIDGGETVEGVPYLVMEYVGGRPLDVFCAEEGLSTEERLKLFSTVCAAVQYAHQH
                     LVIHRDIKPGNILVTAEGLPKLVDFGIAKLLDHDRANATATATALPFMTPDYASPEQV
                     RAEPVTTATDIYSLGVVLYELLAGRRPYRLKTGVPHEIAKAICEDNPPRPSTRQKPLH
                     ADLDNIVLMAMRKEPQRRYATAQQFAEDIQRHLDGLPVRARADTFGYRTGKFIRRHKL
                     GAAAAALITLTLLIGIVATVWQAQVARAERARAERRFNEVRQLANSFVFEVHDAVVNL
                     PGSTTARSLIVQRGLKYLDSLAQDAAGDRGLQRELAGAYEKLGAVQYTPSVAHLGDLA
                     GALESHRKAAALREALVAAEPNNPDYRRELLDSYWFIATLVGRQGDPLQELELIRRQL
                     PAREALAAAEQNSFVDRYNLAGTYVYIGILLMQMDDVQGALENQQEALKLREELLALD
                     PDRTRASRALTISYEYLGLAKDRAGDTKGALELQQRGLEMRESLVNGDPLNTDLRLML
                     VESYRYAGDMYFKLGETKQARDHYSRQFSLVKDMIAADPANPQLLYNEAVAHIKIGDI
                     EAAMGRAASALAKYRRALKIREELSSAAASDSFLRRDLAEVCVKVGDAWALSGNKPEA
                     LASYRRGVDILEALSRAVPANADIRALLAAARTKVG"
     gene            2165..2644
                     /locus_tag="VFO72_04185"
     CDS             2165..2644
                     /locus_tag="VFO72_04185"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9478514.1"
                     /translation="MDELKLTQTPIAKAAMLIRQPVAKVFEAFIDPAITSRFWFTKST
                     GRLEPAKHITWTWEMYDISIEVIIKEIEPNKKIVLDWGNYGEMTTVEWIFTPYEGDAT
                     YVTITNSGFRGDVDKVMRDALDSTGGFTWVLAGLKALLEHNVELNAIADAFPEGLEH"
     gene            complement(2765..>3871)
                     /locus_tag="VFO72_04190"
     CDS             complement(2765..>3871)
                     /locus_tag="VFO72_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9478515.1"
                     /translation="RGAQLNSVTDEGTALMEAVKGGHTEVVKLLLAAGADPKVAHRTG
                     EQALMMAARQRSYQTPAVEPTAEILQLLLVHGADPNATGRWGETALMFANTADKVKLL
                     VAGGAQPEAKDEQGQTALMKAASRGDAGVVSALLDSGANVNAADNTGSNALLHSLDRE
                     NNAHGDERKTLPARRMEVARRLLRAKSIDVNAPNNDGETALMRAVRLENVEMVKSLLT
                     RGADPNRADVFGDTAVTLGYRSAHAEIETALPAAQFKGQPANALNAFLRAAIGRKDEA
                     KVKELLAAGADPNHEYAIGYMHKTIKGRPLVLAASLGHARIVQLLLEKGADINATGII
                     SGSESGLKFGTALEAAELSNHPEVVALLRRTRQN"
BASE COUNT          796 a         1090 c         1115 g          870 t
ORIGIN      
        1 gcggaacgag acgatgagca ttaccgccag caagtcgcga tcaagttgat cagggcgggt
       61 ctgagtggcg aagcgattcg taagcgtttc cggaatgaga tgcagatcct cgccgacctc
      121 aatcatccga atatcgcgcg actgatcgat ggtggcgaga ccgtcgaggg cgtgccgtat
      181 ctcgtgatgg agtacgtcgg cggtcgtccg ctggacgtgt tctgcgctga agaaggtcta
      241 tctaccgaag aacgtctgaa gctcttctcg accgtttgcg ctgcggtcca gtatgcgcac
      301 caacatctgg tcattcatcg cgatatcaag ccgggcaata ttctcgtaac ggctgaaggt
      361 ttgcctaagc tcgtggactt cggcatcgct aagcttctcg accatgaccg agcaaacgca
      421 accgcgaccg cgaccgcgtt gcctttcatg acgcccgatt acgcgagtcc ggagcaggtt
      481 cgcgccgagc ccgtcactac ggcaactgac atctactcgc tcggcgttgt tctttatgaa
      541 ctgctcgccg gccggcggcc atatcgtttg aagacgggcg taccacacga gatcgcgaaa
      601 gcaatttgtg aagacaatcc accgcgaccg agcacgcggc aaaaaccttt gcatgctgat
      661 ctcgacaaca tcgtgctgat ggcgatgcgc aaggaaccgc agcgccgtta cgccacggcg
      721 cagcagtttg cggaagatat tcaacgccat ttggacggac tgcccgtgcg agcgcgtgct
      781 gacacgtttg gctatcggac cggaaaattt attcgccgtc ataaactcgg cgccgcggcg
      841 gctgcgctga taactctgac gctcttgatc ggcatcgtcg cgactgtttg gcaggcccag
      901 gtggcacggg ccgaacgggc gcgtgctgaa agacggttca atgaagtgcg gcaactcgca
      961 aactcgtttg tgtttgaggt tcacgacgcg gttgtgaact tgccgggttc gacgaccgca
     1021 cgttcgttga tcgtgcaacg tggtttgaag tatctcgata gtctcgcgca ggatgcagcg
     1081 ggtgatcgtg gacttcagcg cgaactggcc ggcgcttatg aaaaactggg tgcggttcag
     1141 tacacgccgt cggtggccca tctcggtgat ctggccggcg cgcttgaaag tcatcgcaag
     1201 gccgcagcat tgcgcgaggc attggtcgcc gctgagccaa acaatccgga ctaccgtcgt
     1261 gaactgctcg atagttactg gttcatcgcg acgctggtcg gcaggcaggg cgatccgctt
     1321 caggagttgg aactaattcg ccggcagtta cccgctcgtg aagcacttgc ggcggcggag
     1381 cagaacagtt ttgtcgatcg ttacaacctg gccggcacat acgtctacat cggcatattg
     1441 ttaatgcaga tggatgatgt gcagggtgcg ctcgagaatc aacaagaggc cttaaagtta
     1501 agagaagagt tgttggcgct ggatcccgat cgtacgcgag caagtcgggc gctgacgatc
     1561 tcctatgaat atctgggtct tgcgaaagac cgggccggcg atacgaaggg cgcgctcgaa
     1621 ttacaacaac gtggtctgga aatgcgtgaa tcgcttgtga acggagatcc gctcaacacg
     1681 gatctgcggc tgatgttggt cgagagctat cggtatgctg gcgacatgta cttcaagctc
     1741 ggtgagacaa agcaggctcg agatcattac tccaggcagt tttcgctggt caaagacatg
     1801 attgccgcag atcctgcaaa tccacagttg ctttacaatg aggcggtagc gcatatcaag
     1861 atcggcgata ttgaggcagc aatgggtcga gcagcgagtg cgctggcgaa atatcggcgg
     1921 gcgctcaaga ttcgtgaaga actcagctct gccgctgctt ccgactcatt tcttcgtcgc
     1981 gatctcgctg aggtctgtgt gaaggttggt gatgcttggg ctttgagtgg aaacaaacct
     2041 gaggcgctcg cgagctaccg tcgcggcgtt gacattctcg aggcgctctc gcgcgcggtt
     2101 cccgcgaacg ccgatatccg cgcgctgctg gccgcagccc gcacgaaagt tgggtaacta
     2161 acaaatggat gaactcaaac tgacacaaac gcctattgca aaagccgcga tgttgattcg
     2221 gcaacccgtg gccaaagtgt tcgaggcgtt tatcgatccg gcaatcacct cccgattttg
     2281 gtttaccaaa agcactggac gtttggaacc ggccaagcac atcacgtgga cctgggagat
     2341 gtacgacatc tctatcgagg tgatcattaa agagatcgaa cccaacaaga agatcgtgct
     2401 cgattgggga aactacggcg agatgacaac cgtcgagtgg atcttcactc cgtacgaagg
     2461 cgatgcgact tacgtcacga tcaccaactc cggttttcgc ggagacgtag acaaggtcat
     2521 gcgcgatgcg ctcgattcga caggcgggtt tacttgggtg cttgctggtc tgaaagcact
     2581 gctcgaacac aacgttgaat taaatgcgat agctgacgca tttccggaag gactggaaca
     2641 ttaggagctt tagatttttt gcgatggaag tttttgccga tcggttcggt gcagaagtac
     2701 acgtaattcg actgcattcg atcgttaact tccacacgat gcttcttcat tcgagcttta
     2761 cgcgctaatt ctgccttgtt ctgcgcaaca gggccactac ctcgggatgg tttgaaagtt
     2821 cggccgcttc caacgcggta ccaaacttca gcccgctctc gctgccggag ataatacctg
     2881 tcgcgtttat gtcggcgcct ttctccagca ggagctgcac aattcgcgcg tgacccaaac
     2941 tcgctgcgag caccaggggc cgtcccttaa tcgtcttgtg catgtatccg atagcgtatt
     3001 cgtgattcgg atcggcgccc gccgccaaca actctttgac cttcgcttca tctttcctgc
     3061 cgatggccgc gcgcaggaac gcgttgagcg cgttcgccgg ttgacctttg aactgcgctg
     3121 ccggcagcgc ggtctcgatc tcggcatgcg cactgcggta accgagcgta actgcggtgt
     3181 cgccaaacac gtccgctcga ttcgggtcgg caccgcgggt cagcagcgat ttgaccattt
     3241 cgacgttctc aagcctgacc gcgcgcatca acgccgtttc accatcgttg ttcggtgcgt
     3301 tcacatcgat cgactttgcg cgcagtaacc ttcgcgcgac ttccatacga cgcgccggca
     3361 gcgtcttgcg ctcgtcacca tgcgcgttgt tttcacgatc gagcgaatgc agcagcgcgt
     3421 tggaccccgt gttgtcggcg gcgttaacgt tcgcaccgct atcgagtaga gcgctgacaa
     3481 caccggcgtc gcctctcgac gcggctttca tcagagccgt ttgcccttgc tcgtctttcg
     3541 cttcaggttg cgcaccgccc gcgaccaaca gcttcacttt gtcagcagtg ttcgcaaaca
     3601 tcagagcggt ctcgccccat cgccccgtcg cgttgggatc ggcgccatgg accaatagca
     3661 gttgcaagat ctcagcggtg ggttcgaccg cgggagtttg ataactccgc tgtctcgctg
     3721 ccatcatcaa cgcctgctcg cctgttctgt gggcgacttt tggatccgct ccggcggcaa
     3781 gcaggagttt tacgacctcc gtgtgacctc ccttcaccgc ctccattaac gctgttccct
     3841 cgtcggttac cgagttcagc tgagcgcccc t
//
LOCUS       DASTEY010000234         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_647822, whole genome shotgun sequence.
ACCESSION   DASTEY010000234 DASTEY010000000
VERSION     DASTEY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647822"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..2799)
                     /gene="lon"
                     /locus_tag="VFO72_04195"
     CDS             complement(376..2799)
                     /gene="lon"
                     /locus_tag="VFO72_04195"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9478516.1"
                     /translation="MPESDGNQKFEIPVLALQNLTIFPETVVPLGVGRPRSLAAVEAA
                     LATPEKLLACITVRPDRSDDTDAKAEDLFQIGTLVMIKRMERTGDAMRIVAQGTERIR
                     VIGWKQEDPFLRAEVEILPEARIVDPEEVEATKRNLQQMIQEALSLLPNVPSEVRLAV
                     LSSADPIRLAYFLGSILTLGVEEEQKMLEADTADELLRVAHTNLARELEIMQLRTKIA
                     SEAQVEMDKAQRDYVLRQQMRAIQKELGEDETGEAAEAALLRERLEKADLPDEVRTEA
                     ERELKRLERLPPAAPDYHVIRTYLDFVLELPWRQSSEDKLDLAEARRILDEDHFGLED
                     VKERILEFLAVIKLRPDTKSPILLFVGPPGVGKTSLGRSIARALGRQFERMSLGGVRD
                     EAELRGHRRTYIGAMPGRIIQSIRRAGVNNPVLMLDEVDKLGNDYRGDPSAALLEILD
                     PQQNNSFRDHYLDLPFDLSKIFFIATANQLGPIPAPLRDRMEIIRLAGYSDREKLEIA
                     RRYLVPRQTTENGLNTAQFKISDAAIELIATRYTREAGVRQLERNIGSVARKVALKIA
                     QGEAESVEVDESGVREYLGPPQFYPEQARKELPAGVATGMAWTEMGGEVLFIEATLLP
                     GGRGLTITGQLGEVMQESARAAQSFLWSHAAEFGIDPEIFKDYGVHLHVPAGAIPKDG
                     PSAGVTITSALASLYTGRRVRPDTAMTGEITLSGLVFPIGGVKEKVLAAHRAGIRRVL
                     LPNRNEADVDDIPEDVRTDLQIVFVSTINEVIEAALEVLVANPPPPLMTDSTRGGRTA
                     EPEPLSVKS"
     gene            complement(2805..3170)
                     /locus_tag="VFO72_04200"
     CDS             complement(2805..3170)
                     /locus_tag="VFO72_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9478517.1"
                     /translation="MRNEDRDISVIRRDRRVRPSGRLWNPAADVYRSQEGWVVKLDLA
                     GVCTDELEIKISDSLLRISGCRRDTVYREGFVYQQMEITYSRFEKTIQFPAPIEGASL
                     KHDYRDGFLIIDLHRGEDA"
     gene            complement(3238..4122)
                     /locus_tag="VFO72_04205"
     CDS             complement(3238..4122)
                     /locus_tag="VFO72_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9478518.1"
                     /translation="MARYRGDVNEERDYYPREERFRREALGSYEEPFSRRPRFDDDRR
                     IDNERRFDTERGPEYGRGRYGERADYESGEPRYSSTRERSDDSDRFRDQGRRSSLYDI
                     RGDLSTGRQRDYYAADRSRLRCRDIMTTDLAVAQRGTPLTQVALMMKQEDTGVIPVVE
                     YDVMAGNGRTNEGDRDDRRQDGRNYSRGKLVGLITDRDIVVRAIAEGKECTTTRAEDV
                     MSVDIHTARANDRVVEVTRMMADKQVRRIPVVNENSYLLGMISMADIANEINDDRELG
                     EALEEISKSTSFWNRVFG"
     gene            complement(4276..5019)
                     /locus_tag="VFO72_04210"
     CDS             complement(4276..5019)
                     /locus_tag="VFO72_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET9478519.1"
                     /translation="MKRLLTTTRIMTLVLAAATVLHAQETRTTTSDTPKRQTRITVSG
                     DSIVQAQPDTAILTIAVVTQARRALDAQQQNAAKTDAVVRALKAAAGTGAEVKTSGYS
                     LQPMRIYRENQPPTITGYEARNSVTVTMSDLAKVGDVIDAAGQAGANDVAGISFTLRQ
                     DRPARDRALQEATREAMSKAHALAQALGGRVVSIAEVQEEGFLRPPQPVFRGDVMMER
                     QAAVPTPIEIGSLDITSRVLLVAIVEINP"
BASE COUNT         1036 a         1546 c         1444 g         1203 t
ORIGIN      
        1 tcccagtgtc gggatccatc gccgacgtga gggcgctgac gtcgacaacg atcatggtaa
       61 gcggcatcaa cgatgcagcg acatgcgcgg caaccggtac gccggccaca ccgccttgtt
      121 cagtgacggt gtttggcaca acgctcgagc aaccgcccac tcaatcttta ggcggggcac
      181 tgcatagcag tctgagtgtg ggagaaataa cgatacccac gcctctggct ccgggacaga
      241 gcattaacct gcaattcctg cttggagtac agaagaccgg cttgttcaag ttcttcttca
      301 atatcgaagc gttgccgtaa ggcaaaaaaa gccatccaca gattacgcgg ctttcggtgt
      361 aatctgtgga tggctctagc tctttaccga gagtggctct ggttcggcag tacgaccgcc
      421 gcgtgtggaa tccgtcatga gtggtggcgg cgggttagcg accagtacct ccagcgctgc
      481 ttcgatcact tcattaatcg ttgagacaaa aacgatctgc aaatcggtgc gaacgtcctc
      541 gggaatatcg tccacgtccg cttcgttccg attcggcaac aaaactcgtc gaattcctgc
      601 gcggtgcgcc gcaagcactt tctccttcac gccgccgatt gggaacacga gacccgacaa
      661 cgtgatctcg cccgtcatcg ccgtgtccgg ccgtacgcgc cgtccggtgt acaacgacgc
      721 cagcgccgaa gtgatcgtta cgccggcact tggtccatcc ttagggatcg cgcccgcagg
      781 cacgtgcagg tgaactccgt agtctttgaa gatctccgga tcgattccaa actctgcggc
      841 gtgtgaccac aggaacgact gcgctgcccg cgccgattcc tgcatcacct cgccaagctg
      901 gcctgtgatt gtcagtccac gcccacccgg aagcagcgtc gcttcgatga acaaaacttc
      961 gccgcccatc tcggtccacg ccatgccggt cgcgacgccg gccggcaact ccttgcgtgc
     1021 ctgctctgga taaaactgcg gcgggcccag atactcacga acgccggatt catcaacttc
     1081 aacactttca gcttcacctt gggcgatctt caacgcgact ttgcgagcaa cgctaccgat
     1141 gttgcgttcc aactgccgga cgcctgcttc gcgcgtgtag cgcgtcgcga tcagttcaat
     1201 cgccgcatcg gagatcttga actgggcggt gttcaatccg ttttccgtgg tttgtcgcgg
     1261 caccagatag cggcgcgcga tctcgagctt ttcgcggtcg ctgtagcccg ccagcctgat
     1321 gatctccatg cggtcacgca gcggcgccgg gattggtcca agctggttgg ccgtggctat
     1381 aaagaagatt ttggaaaggt caaacggcag atcgagatag tggtcgcgaa acgagttgtt
     1441 ctgttgcgga tccagaattt caagcagcgc cgcagacgga tcgccacgat agtcgttgcc
     1501 gagcttgtcg acttcgtcca gcatcagcac agggttgtta actcccgcgc gacggatgga
     1561 ctgaatgatt cgtcccggca tcgcgccgat gtacgtgcgc ctgtgcccgc gcaactcggc
     1621 ttcatcgcgc acgccaccca acgacatccg ttcaaactgc cgccccaacg cgcgcgcgat
     1681 cgaacgaccg agagacgttt tgccgacacc cggcgggccg acaaacaaca aaatcggact
     1741 cttcgtgtcc ggccgcaact tgatcaccgc cagaaactcg agaatgcgtt ctttgacgtc
     1801 ttcgagtcca aaatgatctt catcgaggat gcggcgcgcc tctgccaaat cgagtttgtc
     1861 ttcagacgac tgtcgccacg gcaactcgag cacgaaatcc agatatgtgc gaatgacgtg
     1921 ataatccggc gcggccggag gtaggcgttc gaggcgtttt agttcgcgtt cggcctccgt
     1981 gcgtacttca tccggcaaat ctgctttttc aaggcgctcg cgcagcaatg ccgcctcagc
     2041 cgcttcgccg gtctcgtcct caccgagttc cttctgaatc gcgcgcatct gctggcgcaa
     2101 cacgtaatcg cgttgcgcct tgtccatctc aacctgcgct tccgaagcga tcttcgtccg
     2161 cagttgcatg atttcgagtt cgcgggcgag gtttgtgtgc gcgactcgca gcagctcgtc
     2221 ggcagtgtct gcttcgagca tcttctgctc ttcttcgacg ccgagagtga gtatcgaacc
     2281 gagaaaatac gccagccgga tcggatccgc ggaactgagc accgcgagcc tcacttcact
     2341 gggaacgttc ggcagcaggc tcaacgcttc ctggatcatc tgctgcaggt ttcgcttcgt
     2401 cgcttcaacc tcttcgggat cgacaatgcg agcttccggc aggatctcga cttcagcacg
     2461 caggaatgga tcttcctgtt tccaaccgat tacgcggatg cgttcagttc cctgagccac
     2521 gatgcgcatg gcgtcgcccg tgcgttccat gcgcttgatc atcaccagcg ttccgatctg
     2581 gaagagatct tccgccttcg catcggtgtc gtctgaccga tcaggacgca cggttatgca
     2641 agccaaaagc ttctcgggcg tggccagtgc agcttcgacg gcggccagcg atcgaggccg
     2701 gccgacgccc agcgggacta ctgtctccgg aaatatggtg aggttttgca gtgccagcac
     2761 cgggatctca aatttttgat ttccgtcaga ttcaggcata acgtctacgc atcttcccca
     2821 cgatgcaagt cgataatcaa aaaaccatcg cgataatcgt gtttaagtga agcaccttcg
     2881 atcggcgccg gaaactggat cgtcttttca aaacggctgt aagtaatctc catctgctgg
     2941 taaacgaaac cttcgcgata aacggtgtcg cgccggcaac cgctgatgcg cagcagcgaa
     3001 tcactgatct tgatctccag ctcgtcagtg caaacgccgg ccagatcgag tttgaccacc
     3061 catccctctt gcgaacgata cacatcggcg gctggattcc agagacgccc ggacggtctc
     3121 acccgccggt cccggcggat taccgagata tcacgatctt catttcgcat gacaaaagaa
     3181 tatccacaga ctacacggat tgtaaatcag tgtaatctgt ggatgatttt agaagaatca
     3241 gccgaagacg cgattccaaa acgaagtgct tttggagatc tcctccagcg cctcaccgag
     3301 ttcgcgatcg tcattgatct cattcgcgat atcggccatc gagatcattc cgaggagata
     3361 gctgttctcg ttaaccacag gaatgcggcg cacctgtttg tccgccatca tgcgggtaac
     3421 ttccaccacg cgatcgttcg cgcgggcggt gtgaatatcc actgacatga cgtcttccgc
     3481 gcgagtggtc gtgcactctt tgccttcggc gatggcgcgc acgacgatgt cgcgatccgt
     3541 aatcaggccg acgagttttc cgcgcgagta attgcgcccg tcttgccggc ggtcgtcacg
     3601 atcgccctcg ttcgttctgc cgttgccggc catcacgtcg tactcgacca cgggaatcac
     3661 gcccgtatct tcctgtttca tcatgagcgc gacttgcgtt agcggcgttc cacgctgtgc
     3721 tactgcgaga tcggttgtca tgatgtcgcg gcagcgcagg cgagaacggt cggcggcgta
     3781 gtaatcgcgc tggcgtccgg tcgagaggtc gccacgtatg tcgtacaacg aactgcgccg
     3841 tccctgatcg cggaagcggt cgctgtcgtc cgaccgctcg cgagtgctgc tgtagcgcgg
     3901 ttcgccggat tcatagtcgg cacgttcgcc gtagcgaccg cggccatatt cggggccgcg
     3961 ttcggtgtca aaccggcgtt cgttatctat gcgtcgatca tcgtcgaatc ggggacggcg
     4021 gctgaatggt tcttcgtatg agcctaatgc ttcacgtctg aaacgttcct cccgcgggta
     4081 ataatccctt tcctcattca cgtcacctct atatctggcc atttgtttcc ctccttaacc
     4141 tgtaacataa atgctcgggg gcaaggggtt ttgacatgat tacagaaata acaggatggt
     4201 tttctacgaa gtgaaggata gcctgatcag gttttaccgc ttgggacatc ttgtaaactc
     4261 tgtgaatcat gtcaactagg ggtttatctc cactatcgca accagtagca cgcgcgaagt
     4321 gatgtcgagc gagccgatct caatcggggt cgggacagcg gcctgcctct ccatcattac
     4381 gtcacctcga aaaaccggtt gcggggggcg taagaaacct tcctcctgaa cttcagcaat
     4441 actcaccacg cggccgccga gtgcttgagc caacgcgtga gccttgctca tcgcttcgcg
     4501 cgtggcttct tgcagtgcac gatcgcgagc cggacgatcc tggcgcagcg tgaaggatat
     4561 tcctgccacg tcattggctc cggcctgccc tgccgcgtcg atcacgtcgc cgacttttgc
     4621 caagtcactc atcgtcacgg taacgctgtt acgtgcttcg tagcctgtga ttgtcggcgg
     4681 ctggttttcg cggtagatgc gcatcggttg caggctgtaa ccgctggttt ttacttcggc
     4741 gcctgttccg gctgcggctt ttaaagcgcg gactaccgcg tcagttttcg ctgcgttttg
     4801 ctgttgcgcg tcgagagcgc gtcttgcctg ggtgacgacg gcgattgtta gtatcgccgt
     4861 gtcgggttgg gcctggacga tcgaatcacc tgagacggtg atgcgagttt ggcgttttgg
     4921 agtgtcgctg gttgtggttc tggtttcttg ggcgtgcagg acagttgcgg ctgcaagcac
     4981 gagggtcatt attcgggttg tggtcaacaa tcgtttcata atctcacctt ccgttttctc
     5041 tagtcttcac ggcttgggag agattaatag gccgcggacg tagagaacgc gccggaagca
     5101 acatcttgcg tgttttgtta ccatccattc aatgcaatca ttcaatactg aaacagacga
     5161 agccccgcca accgaaagcg ccaaacaaac tttgattgta ggcacaaccg gcgatctgcg
     5221 acccggcgc
//
LOCUS       DASTEY010000235         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_911535, whole genome shotgun sequence.
ACCESSION   DASTEY010000235 DASTEY010000000
VERSION     DASTEY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911535"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="VFO72_04215"
     CDS             <1..1573
                     /locus_tag="VFO72_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9478520.1"
                     /translation="DVSDLKRAEDSLSALKEARTNAAQQKDINTERKRAEKALRESEE
                     RYRDLVENSHELICTHDLDGTILSVNRAARELFGYELREFIGKKNIRDILAPKVRDQF
                     SMYMQRILKEGATSGTMLVQTRSGEHRLLEYYNSLRTEGVAAPIVRGIARDITTTRRA
                     EHALRESEERYRELFENSKDAFYVHDIDGIYTSVNRAAEKLSGYSREQIVGKHFSEFM
                     TPEHARHVQRQLLKKLESTSETTYEIEMITKKGRHVPVEISSRLIFENGVPVGVQGCV
                     RDISERKKAQEAARNYSRRLIEAQEAERRRISRELHDQVGQILTAVKMNLHVLRHKCT
                     QPEILLSINDNLKVIDEAVDQIRDLSVDLRPLLLDDLGLVVALRWYLERQTRNLGIPA
                     KFVSGSLDEDDRFSSELETACFRIVQEGVTNIVRHARATRISVRLERVISDLILLITD
                     DGAGFDARMLRSGNGMSTLGLRGMEERAQAVGGTITIDSAPALGTEICARFPIKGEKR
                     RDADSGKAVEVAAKV"
     gene            complement(1751..2398)
                     /locus_tag="VFO72_04220"
     CDS             complement(1751..2398)
                     /locus_tag="VFO72_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9478521.1"
                     /translation="MKPIRVLIADDHALVRAGIRALVERIKGVVVVGEAGKGSEALKL
                     IDELQPHLILLDITMPDGSGFDVLEHVAKKLPNIRVIVLTVHEAGEYAIRALREGAAG
                     FLPKSAASTELEQAIQTVMKGDVYISPETSRRTLIEYGKGVSKRDLLATLSPRQREVL
                     QLIAEGRTTKQIAQLLDISVKTVETHRAQLMERLNIHDVAGLVRYAIIVGLIEVE"
BASE COUNT          708 a          833 c          845 g          639 t
ORIGIN      
        1 ggacgtctcc gatcttaaac gggccgaaga ttcgttaagc gcgctcaaag aagccaggac
       61 gaatgcggca cagcagaagg acatcaacac agaacgcaaa cgcgcagaaa aggcgttgag
      121 agaaagcgag gagcgttatc gcgatctggt ggaaaacagt cacgaactga tctgcactca
      181 cgatctcgac ggaacaattc tttcggtcaa ccgtgcggca cgcgaactct ttggctacga
      241 gctgcgcgag ttcatcggca agaagaacat ccgtgacatc cttgcgccga aggtccgcga
      301 tcagttcagc atgtacatgc agcggatttt gaaagaaggc gcgacgagcg gaacgatgtt
      361 ggtacaaaca cgctcgggcg agcatcgcct gctcgagtat tacaactcgc tccgcacgga
      421 aggcgtagcc gcgccgatcg tgcgcggcat tgctcgcgac atcacgacga cgcgccgcgc
      481 tgagcacgcg ttgcgcgaaa gcgaggagcg ctatcgcgag ctctttgaga attcaaaaga
      541 cgctttctac gttcacgaca tcgacggtat ttacacctcc gttaaccgcg ctgctgaaaa
      601 actcagcggg tacagccggg aacagatcgt cggaaagcat ttttctgagt tcatgacacc
      661 cgagcacgcg cggcacgttc agcggcaact tctgaagaaa ctcgaatcca ccagtgaaac
      721 cacctacgag atcgagatga tcacgaaaaa gggaagacac gtaccggtcg agatcagcag
      781 ccggctgatc ttcgagaacg gtgtaccggt cggcgtccag gggtgcgttc gcgatatttc
      841 cgaaaggaag aaagcgcagg aagcagcgcg caactattcg cggcggttga tcgaagcgca
      901 ggaggcggag cgacgcagga tatcgcgcga gctccacgat caggtcggac aaattctgac
      961 ggccgtaaag atgaatcttc acgtgttgcg gcacaagtgc acgcaaccgg agatactgct
     1021 gtcgatcaac gacaacctga aagtgatcga tgaagcagtc gatcagatac gagacctttc
     1081 agtcgatctg cggccgctgc ttctcgatga ccttgggctg gtagtcgctt tgcgctggta
     1141 cctcgagcgc cagacaagaa atcttggtat tccggcgaag tttgttagcg gttcgctcga
     1201 tgaagacgat cggttttcat cggaattgga gaccgcgtgt ttccggatcg ttcaggaagg
     1261 cgtgacaaac atcgttcgcc acgcgcgcgc gacgagaatc tcggtgcgat tggaacgtgt
     1321 catttccgac ctgatccttt tgattacaga cgacggcgcg ggtttcgatg cgcgcatgct
     1381 gcgcagcggc aatggtatgt caacgctggg attgcggggg atggaagaga gggcgcaggc
     1441 agtgggaggg acgatcacga tcgactccgc accggccctg gggaccgaga tttgcgcccg
     1501 cttcccgatc aaaggtgaaa agcgccgcga tgcagactcc ggcaaagcgg tcgaggtcgc
     1561 ggctaaggtc tgaactaccc accgtgaaat cgcaaatcaa ttagtggatt ggttcatgcc
     1621 cggtccaata caagtcctca ggacttgaga acgatcacgc tactggtgat cgcgctagag
     1681 cgcgtggggt ggtctctctc gcagaccgga catgaaccca tccaccaatt cctcatctgg
     1741 caactcgcgt tcattcaact tcaatgagtc cgacaatgat cgcgtaacgc acgagccctg
     1801 cgacatcatg aatattcaac cgttccatca gttgcgcgcg gtgcgtctca accgtcttca
     1861 cgctgatatc caaaagctgc gcgatctgtt tggtcgttcg accttcggcg atgagttgca
     1921 gcacctcgcg ctgtcgcggg ctcaacgtcg ccaacaaatc tctcttcgaa acacccttgc
     1981 cgtactcgat cagcgtcctt cgcgaagttt ccggtgagat gtaaacatct cccttcatca
     2041 ccgtttgaat tgcctgttcg agctcggtgc tggccgcgct cttgggcaaa aaccccgccg
     2101 ctccttcgcg caacgcgcgg atcgcatact cacctgcctc atggaccgtc aggacgatca
     2161 cgcggatgtt gggtaacttc tttgccacgt gctccagcac gtcaaaaccg ctgccgtcgg
     2221 gcatcgtaat gtctaacagg atgaggtgcg gctgaagctc atcgatcagt ttgagtgctt
     2281 cacttccttt gcccgcctcg ccaaccacca cgacgccctt gattcgttcc acgagggccc
     2341 tgattcctgc acgcaccaga gcgtggtcgt cagcgattag cacccgtatc ggcttcatct
     2401 cgtggcttca tctcgtagtt gtgccgtgct ggtggtctat ctccggcaaa atgcgttcac
     2461 tagtgataag ggcaaaaagc cgagcgcctg gagcttgagt aagagcgaaa gcagaaactt
     2521 cgcgctcgct tgcaggggca taattgcagg aagctccctg atccaacaat caggaattac
     2581 ctgagtctga ccgaaacccg tttttggtca tcgcagttta gtgtcttcaa acagcgggcg
     2641 gtgaagaata gctgtatcga tgaagtgtgc gctacgtttt cgcgggcctt cagcagttaa
     2701 atttttaggc tgcaaaactg ctcaggaatt atctgagctg tgttcaggaa actcctcatt
     2761 gcgcacactc aacagagcgc ttagcctaac cgggcgaatt ggttccatag cccccatgcc
     2821 ctattgagcg tgttccaacg ccactctttt cgcaccctca tatcaagagg gcacggctat
     2881 gaccacaaag aatcgatccg tgcggtgtct gtttaggaac gcgaacagac gcaatctttt
     2941 ctatcgcgcg cagctgaagg tatgagcact gctccgaggt acggacggtg attgattgag
     3001 ttctgagaga cgcgcgcagt ggtct
//
LOCUS       DASTEY010000236         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_680825, whole genome shotgun sequence.
ACCESSION   DASTEY010000236 DASTEY010000000
VERSION     DASTEY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680825"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..857
                     /locus_tag="VFO72_04225"
     CDS             243..857
                     /locus_tag="VFO72_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET9478522.1"
                     /translation="MDLRNLVCRTLVTLAVVALCSISAFSQTQPPVSQPINDPNEISC
                     SPDDFALLSAAEDSKPVWEAKPKPVIDVAAPVGPTNTGLMKFQPLLMAAIGARLGARY
                     RWGATGPDRFDCSGFVWSIFQATGINFERVSARHLFARFAQPAPEEQYKFGTLVFFSR
                     LTHVGVVADEHGFYHASRRHGVVYQPFTEYWLKRIDGFRRVPLS"
     gene            complement(931..1344)
                     /locus_tag="VFO72_04230"
     CDS             complement(931..1344)
                     /locus_tag="VFO72_04230"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9478523.1"
                     /translation="MGDTTAGVKPRSYYDCVDCPAYCCSVYERVQVTPRDIRRLARHF
                     GVTEEVATARYTKIYEKERVLRRKRDHLFGEACQFINPETRGCTIYHARPAVCREFPI
                     TTRCAYYDLLRFEREQQDDPKALPIVQITFRNGKK"
     gene            complement(1368..>2084)
                     /locus_tag="VFO72_04235"
     CDS             complement(1368..>2084)
                     /locus_tag="VFO72_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET9478524.1"
                     /translation="TDAFFRRIIEGPEFQRSMSTAEFHYLCGRYVREREQKLGTKDKS
                     LLAVAYEYGVPIYTSSPGDSSIGMNVAAKALRGNKLAFDPSLDVNETAAIVLAAKRAV
                     HNAKSGKSKKQKGRSAVFILGGGSPKNFLLQTEPQIQEVLGIDERGHDFFLQVTDARP
                     DTGGLSGATPGEAVSWGKVDPDRLPDAVVCYVDSTVALPLITAYALTRHEPREPKRLY
                     EKRDQFMNLLQAEYKKSKRR"
BASE COUNT          455 a          561 c          560 g          508 t
ORIGIN      
        1 tacagcgtgc gtgagtcgcc gcgaggctac aaatctttca gattgaccgc gctcgtaact
       61 gattcgtgac ttctttcaat gcgcgaaaag atcgcggtgc gcgtttcgtc acaaaacagt
      121 cacattgcca gttgaaacac gcattttcag cagctaaact gccgacgtct tttagcggtg
      181 ccctcctcac tcaagcaata aacaaagtcg aattagctaa ggagaaggtg attccccgta
      241 ttatggattt acgaaatctc gtctgtcgca ctttggttac gctcgcggtt gttgctcttt
      301 gttcgatatc agcgttctcg caaactcagc cacctgtttc gcaaccgata aacgacccga
      361 acgagatctc gtgctcgcct gatgattttg cgctgctctc agcagcagaa gattcaaagc
      421 ctgtctggga agcaaaaccg aaaccggtaa tcgacgtcgc tgcaccggtt ggtccaacca
      481 acaccggtct gatgaagttt cagccattgc tgatggccgc gatcggtgcg cgcctgggtg
      541 cgcgttatcg ctggggagcc actgggccag acagattcga ctgttcgggt tttgtgtgga
      601 gcatctttca ggcaaccgga attaacttcg aaagagtcag tgctcgccac ctctttgctc
      661 gctttgcgca gcctgcgccg gaagagcagt acaaattcgg cacgctcgtg ttcttcagcc
      721 gactcacaca cgtcggcgtt gttgctgacg agcatggttt ctatcatgcg tcgcgccggc
      781 acggcgtcgt ttaccagccg ttcactgagt attggttaaa gcggattgat ggattccgtc
      841 gcgtgccgct gagttagatt tttgattttt gattgtaaag caaggaacaa tcggcaatcg
      901 gcaatcggca atcaaaaatc aaaaattcct tcacttctta ccattgcgaa aagtgatctg
      961 tacgatcggc agggctttgg gatcgtcctg ttgttcgcgt tcaaaccgta gcaggtcgta
     1021 gtacgcgcag cgcgtggtaa tcggaaactc acggcacacc gcgggacgcg cgtgatagat
     1081 tgtgcagccg cgtgtctcgg gattgatgaa ctggcacgcc tcgccaaaca ggtgatcgcg
     1141 tttccggcgt agcactcgtt ccttctcgta tatcttcgtg taacgcgccg tcgctacttc
     1201 ttccgttacg ccgaagtgtc gcgcgaggcg acgtatgtcg cgaggcgtta cctggacgcg
     1261 ttcatagacc gaacagcagt atgccggaca atcaacgcag tcgtagtagg accttggctt
     1321 aacgcccgca gtagtgtctc ccatgatcgc cgcgtctccc ctcaaagcta gcgtcgtttt
     1381 gattttttat attcggcttg caacaggttc atgaactgat cgcgcttctc atagaggcgc
     1441 ttcggttcac ggggctcatg acgcgtaaga gcataagccg taatgagcgg aagcgccaca
     1501 gtagaatcca cgtaacacac gaccgcgtcg ggaagtcgat cgggatcgac tttaccccac
     1561 gacacagctt cacctggcgt tgcgcctgaa agacccccag tgtcgggtcg cgcgtctgtc
     1621 acctgtagaa aaaaatcgtg gccgcgttcg tcgataccga gcacttcctg aatctgcggt
     1681 tcggtctgta acagaaaatt cttaggcgag ccgccgccga ggataaagac tgcggaacgt
     1741 cctttctgtt tcttgctctt tccacttttc gcgttatgta cggcacgctt ggccgcgagc
     1801 acgatcgctg cggtttcgtt tacgtcaagc gagggatcga aagcgagttt gttgccccgc
     1861 agtgccttgg cggcgacgtt catgccgatt gaagaatcgc ccggagagga agtgtagatt
     1921 ggcacgccat actcgtaagc aaccgccagc agcgacttgt ctttagtacc gagtttctgt
     1981 tcgcgctcgc gaacgtaacg gccgcataag tagtgaaatt cagcggtaga catcgaacgc
     2041 tgaaactccg gaccttcgat tatgcggcgg aagaaagcgt ccgt
//
LOCUS       DASTEY010000237         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1257662, whole genome shotgun sequence.
ACCESSION   DASTEY010000237 DASTEY010000000
VERSION     DASTEY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257662"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..646
                     /locus_tag="VFO72_04240"
     CDS             68..646
                     /locus_tag="VFO72_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478525.1"
                     /translation="MSYIQFADEPNQEERIAKLRKEVEKLGGSTMSLESMPADMEEEF
                     LRHVLEYEKAEPISLFKLLENSGLKIPPPDQFDDENLTIKLKEIIDRMASLGAYLLHT
                     NHLSDRALYEFLYYDGLREEAVLFPENPSYAYMIDLTGSGSEEDNQVFLKYYADEKYR
                     GQWANDWPDDRIPEHEDPPFDRDRFLPRSPLG"
     gene            complement(767..1669)
                     /gene="argB"
                     /locus_tag="VFO72_04245"
     CDS             complement(767..1669)
                     /gene="argB"
                     /locus_tag="VFO72_04245"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET9478526.1"
                     /translation="MNVKIDNQTRLDLLREALPYIQRFQGQTFVVKLSGKVTENHDNL
                     ASLAEELALLHQVGIRICVVHGGGKQLSELASKLGVQQTIIEGRRVTDDATLEMAKMI
                     FAGKINTDILAALRQRGIEAVGLSGVDGNIVHAEKRPPREILNRQTGVRDTVDFGYVG
                     DVVQINSRLLTVLLDHGYLPVISSLGADAEGMVFNINADTIASEIAVQLQAEKLILLS
                     DVDGIYLKPDDPQTKLSRLSAAEADQLVAGGAATGGMIPKLQSITTLLRRGVHSAHII
                     SGTNRNALLSEVFTDKGTGTMIVA"
     gene            complement(1666..2649)
                     /locus_tag="VFO72_04250"
     CDS             complement(1666..2649)
                     /locus_tag="VFO72_04250"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HET9478527.1"
                     /translation="MNFLNTLDSNLDELIDSALRFKHGDSHVNSLAGKSVALVFFNPS
                     LRTRASMQVGIYELGGNAVVLEPGGTSWTLEHREGAVMDAGKIEHVAEFVRVLERYCV
                     AIGVRTFAALKNWQEERTDPVLNAFAKYASVPIINLESAMHHPCQSLADMMTIREKLG
                     PGRKRVVLTWAWHPKPLPMAVPNSFALAAAQMGHDLVIAYPPGYELDEEILDTTRQHA
                     AQAGGSVEITNDIEAAFTGAEVIYAKSWGGKQFYGNTEGDLAERAQYRGQWIVNEDRM
                     GRTQNGIFMHCLPVRRNVIVTDGVIDSQSSVVIDEAENRLHVQKAVMAQIL"
BASE COUNT          579 a          771 c          777 g          622 t
ORIGIN      
        1 aggctcctca cagcaactcc gaaggcgcct caaaccttaa acctctcaat ttcttaagga
       61 gccaagaatg tcgtacatac aattcgccga cgaacccaat caggaagaac gtatcgccaa
      121 actccgcaag gaagtggaaa aactcggcgg cagcacgatg tcgctcgaat cgatgccggc
      181 agatatggaa gaagagttcc tccggcatgt gctggaatat gaaaaggccg agccgatcag
      241 tcttttcaag ttgctcgaaa actcgggtct taaaatcccg ccgccggacc aattcgacga
      301 tgaaaacctc acgatcaaac tgaaagagat catcgaccgc atggcgtcgc tcggcgcata
      361 tttactccac accaatcacc tctcggatcg cgcgctttac gagtttcttt attacgatgg
      421 cttgcgtgag gaggccgtgc tgtttccgga aaacccgagc tacgcttaca tgatcgatct
      481 cactggcagt ggtagcgagg aagataatca ggtttttctg aagtattacg ccgacgagaa
      541 ataccgcggt caatgggcca acgactggcc ggatgatcgg atacccgagc acgaagatcc
      601 accatttgat cgcgatcgct ttctaccgcg atcgccgctc ggctaacgtc agaaaccggt
      661 cgtcccgatc ctgggaccgc aggcgtctcg cctgcacgcg ctacgcgtcg ggcgcttccc
      721 ggacttagca ggcgagacgc ctgcggtccc agggtttagt ccgtggctaa gccacgatca
      781 tcgtgcccgt tcctttgtcg gtgaagactt ccgaaagcag tgcattgcga tttgtgccgc
      841 tgatgatgtg ggcggagtga actccacggc gcaacagcgt agttatgctt tgcagcttgg
      901 ggatcattcc gccggtggcc gcgccgccgg cgacgagttg gtcggcctcg gcagcgctga
      961 gtcgcgacag ttttgtttgc ggatcgtcgg gtttcaaata gatgccgtct acatcgctaa
     1021 gcaggatcaa cttctcggct tgcaactgga ctgcgatctc ggaggcgatc gtgtcggcat
     1081 tgatgttgaa gaccatacct tccgcgtctg ctccgagcga agagatcacg gggaggtagc
     1141 cgtggtccaa gagtaccgtg agcagccggg agttgatctg cactacgtct ccgacgtaac
     1201 cgaagtcaac tgtgtcgcgc acgccggtct ggcggttcag tatttctctg ggcggacgtt
     1261 tttcggcgtg aacgatgttg ccgtcgacac ccgaaagacc gacggcttca atgccacgct
     1321 ggcgcaacgc cgcaaggatg tcggtgttga ttttgccggc aaagatcatt ttcgccattt
     1381 cgagtgtggc gtcgtcagtc acgcgacggc cttcgatgat cgtctgctgg acgccgagct
     1441 tggaagcgag ttcgctgagt tgcttaccgc cgccgtgcac gacgcagatg cgaataccga
     1501 cctgatggag cagcgcgagt tcttcggcga gtgacgccag gttgtcgtgg ttttccgtga
     1561 ctttgccgga gagtttgacg acaaacgtct ggccctggaa gcgctggatg tacggaagcg
     1621 cttcacgcaa cagatcgagg cgagtttggt tgtcgatctt tacgttcata ggatctgtgc
     1681 catcactgct ttttgaacgt gcagccggtt ctccgcttcg tcaatgacta cggaggattg
     1741 tgagtcgatg acgccgtcag tcacgatcac gttgcgccgt acgggaagac agtgcatgaa
     1801 aatcccgttt tgggttctgc ccatccggtc ttcattcacg atccactgac cgcggtattg
     1861 ggcgcgctcc gccaaatccc cctccgtgtt gccgtaaaac tgtttccctc cccaactctt
     1921 agcgtaaatg acttcggcgc cggtaaaagc ggcttcgata tcgttagtga tctcgacgct
     1981 gccgcctgct tgcgccgcgt gttgtcgcgt ggtgtcgagt atttcttcgt cgagttcgta
     2041 accgggcgga tatgcgatga cgagatcgtg gcccatttgc gcggccgcta atgcgaaact
     2101 gtttggtacg gccatcggca atggcttcgg atgccaggcc caggtcagca ccacacgctt
     2161 gcgtccaggg ccgagcttct cgcgaatcgt catcatgtca gccagagatt gacagggatg
     2221 gtgcatcgcg gattcgagat tgatgatcgg cactgatgcg tattttgcga aggcgttcag
     2281 cacaggatcg gttcgctctt cctgccagtt tttcagtgcg gcgaacgtgc gcactccaat
     2341 cgcaacgcag tatcgttcga gcacgcgcac aaattcagcg acatgctcaa tcttgccggc
     2401 gtccatgacg gcgccttcgc gatgttcgag cgtccagcta gtgccgccgg gttcaagcac
     2461 tacggcgttg ccgccgagtt cgtaaatacc gacctgcatg gaagcgcgtg tgcgcagact
     2521 cgggttgaag aagaccagag ccacggactt acccgcgagg gagttgacgt gactatcgcc
     2581 atgcttgaaa cgcaaagcgg agtcaatcaa ctcatccagg ttgctgtcga gcgtgttcaa
     2641 aaaattcatc cgtgttcatc cttgttctaa cgcactcaca aataaatcca cctccgctgt
     2701 ttgcaaacac aacggcggca acaaccgcag cacctttggg tcactcgcc
//
LOCUS       DASTEY010000238         4793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1191788, whole genome shotgun sequence.
ACCESSION   DASTEY010000238 DASTEY010000000
VERSION     DASTEY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191788"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFO72_04255"
     CDS             <1..470
                     /locus_tag="VFO72_04255"
                     /inference="COORDINATES: protein motif:HMM:NF027856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478528.1"
                     /translation="DRDHVWHMQTLKSICDAGEGLLLATSYMIVDVTDLASVEAGISW
                     WEELTGGGGEGMVVKPFDFITRSSRGLVQPAVKCRGREYLRIIYGPEYALPENLERLR
                     ARGLSTKRSLALREFALGIEGLERFVRHEPLRRVHECVFGVLALESEPVDPRL"
     gene            complement(507..1064)
                     /gene="mug"
                     /locus_tag="VFO72_04260"
     CDS             complement(507..1064)
                     /gene="mug"
                     /locus_tag="VFO72_04260"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HET9478529.1"
                     /translation="MTKPWKPTKEQLLAATEKTVRDVIAPNLRVLFCGINPGLYTAAV
                     GHHFARPGNRFWPALYAAGFTDRLLSPFDERELLKSGYGITNVVPRTTASADQLTREE
                     ILAGGEQLKAKVLRYRPRVLALLGVGAYRTAFNQPKATVGRQPNTIGDTVLWVLPNPS
                     GLNANYQAADLARLFRELKEFSDSC"
     gene            complement(1074..3194)
                     /locus_tag="VFO72_04265"
     CDS             complement(1074..3194)
                     /locus_tag="VFO72_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478530.1"
                     /translation="MCAFLTAIEAAGQTTQTPPTKSADEVVRVYTELVQTDVMVFDKQ
                     GRFVDGLTKENFELRIDGKPRPIQAFEKITAGSDEESQLAAARGATTVNLKRPVPLDR
                     GRIVFFYLDDFHMDLAGMLAAKKVISTFIDKQMGQNDQAAIASATGQIGFLQQLTHDR
                     MVLHTALDRLTPRSYTVRDFDRPPMSEYEALMIERMDRSVFEFFVNETLRTNPGIRRD
                     TAEQIVRQRAQSITTQAARLNFNTLSGLESLVRGARNLPGRKVLFFLSGGFLLENRRG
                     DSMTKLRDITNAAAKSGVVIYSMDTRGLVATLQDASTEQAFDPSGQLQSARFGELSAS
                     QDGLHALAADTGGKAIFNTNELSQGLAPAIAETSSYYLLAWKPDAVSQKQRRFRQLEV
                     KLVGRPDLTVRVRKGFFDLDPVPAVAANKKTESATPTKTPTAKLREAIAAPYPVRNLP
                     ILMSVDYYDVAPKGPTLSTAIQLPGEFLSFGEQPGGKIQAIVDVSGIYYDDKGVAKAS
                     FLERIVTTAPSLEATKTYSSDIIFTYPATLPPGLYQVRAAARDEKSGSVGSAHAWVEI
                     PDLSKKQLTMSSVLLGERSSTATNVSAPNAANPVSLSASHRFKRDSTLRFLIFAYNTK
                     PSPADQKPDVAVQVQVIRDDQPVITTALRKINTEGVADPTRLPYAAEISMSELLPGRY
                     VLRVSIIDRVVKQSTSRQTYFDVY"
     gene            3216..4169
                     /locus_tag="VFO72_04270"
     CDS             3216..4169
                     /locus_tag="VFO72_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9478531.1"
                     /translation="MTFETGKHKAGIMMAETGVTVQLNRVSKSFGDFTAVKELSVTVR
                     AGRVFGLLGPNGAGKTTTIRMIVNITAPDSGKIELFGGHINSALQDRIGYLPEERGLY
                     RRMKIVDQLRFFAELKNVRGKEVETKIDQWLARVKLSEWKNKRSMELSKGMQQKIQFV
                     TAVLHDPDLLILDEPFSGLDPINLELLKEIVLDLKRSGKTIIFSTHQMEVAEKLCDDI
                     CLLNRSQKVFEGSLREIKSSFGRNAVAVRCEGGDGVLDDPTLVSKLVRHADEAQALLA
                     EGADAQVLLRRLIDSGARIGKFEMVEPSLNDIFITKVTEHA"
     gene            4166..>4793
                     /locus_tag="VFO72_04275"
     CDS             4166..>4793
                     /locus_tag="VFO72_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478532.1"
                     /translation="MRKFLAVLKREYTQRVRAKMFIVSTILLPLVMSLFGIVPAVILS
                     IEAAPMRVAVIDQTGKIYAHLEEALASDESKSDEPDQTRTGPEVAGRTFHLEQVAAIG
                     PEANEERARLEARLQNKELDGYLVIPAEFLQHGQVEFFNNNPGDVLSRRILQSALNRA
                     AREERLVEARVDAETRQGLFQPVNLQSVKIGAAGEERDSGGSFILVFGV"
BASE COUNT         1034 a         1270 c         1446 g         1043 t
ORIGIN      
        1 cggatcgcga tcacgtctgg cacatgcaaa ctctgaagag tatttgcgac gccggcgagg
       61 gactgctgct ggccacgtcc tacatgattg ttgacgtcac ggacctggcg agcgtggaag
      121 cgggcatcag ttggtgggaa gagttgaccg gcggcggtgg agagggcatg gttgtcaaac
      181 cttttgattt catcacacgc agctctcgtg gcctggtcca gccggcggtg aagtgcaggg
      241 gtcgggagta tctacgcatc atttatggac cagagtacgc gttgccggag aatctcgaac
      301 gcctgcgcgc tcgtggatta tcaacgaagc gctcgctcgc gttgcgcgag tttgccctcg
      361 gcatcgaagg actcgaacgc tttgtgcgtc acgaaccgct gcgccgcgtt cacgagtgtg
      421 tgtttggcgt cctggccctg gaaagcgagc ccgttgatcc gcggctgtaa gccggcagcg
      481 acagacgagc cacgggcaga cacgaactaa cacgaatcag aaaattcttt cagttcccga
      541 aacaaacgtg ccagatccgc ggcttggtaa ttggcgttga ggccgctcgg atttggtagg
      601 acccagagca cggtgtcgcc gatcgtgttt ggttgccggc cgaccgttgc tttcggttga
      661 ttgaacgccg tgcgataagc gccgacaccc agcaacgcca gtacgcgcgg tcgataacgc
      721 agcaccttcg ccttcaactg ttctccaccc gcgaggattt cttctcgagt aagctgatca
      781 gctgaagcgg tcgttcgtgg gaccacgttg gtgattccgt aaccactctt cagcagttcg
      841 cgttcgtcga aaggcgacag tagccggtca gtaaatccgg cagcgtaaag cgcgggccaa
      901 aaacgattcc ctggccgcgc aaagtgatgg cccaccgcgg cggtgtaaag tccgggattg
      961 ataccgcaga acaggacacg cagatttggc gcgatcacgt cgcggaccgt cttttcggtc
     1021 gcggcgagaa gttgctcttt ggtcggtttc cacggtttcg tcacgatgtg aggttaatag
     1081 acgtcgaagt acgtttggcg cgaggtgctt tgcttaacca cgcgatcgat tatcgagact
     1141 cgcagcacgt agcggccggg caacaattca ctcattgaaa tctcagcggc gtagggaagg
     1201 cgcgtcggat ccgcgactcc ttcagtattg atcttgcgca acgcggtcgt gatgacaggc
     1261 tgatcgtctc gtattacttg aacctgcacg gcgacgtccg gcttttgatc cgccggcgac
     1321 ggcttcgtgt tgtaagcaaa gattagaaaa cgcagtgtcg aatcgcgctt aaagcggtga
     1381 cttgcactca gcgaaaccgg gttggccgcg ttgggagcag aaacattagt tgcggttgac
     1441 gatcgctcac ccaggagcac actgctcatc gtcaactgct tcttcgacaa gtcaggaatc
     1501 tcgacccacg catgcgcact accaacactg ccactcttct cgtcgcgcgc agctgctctg
     1561 acttgataaa gcccgggtgg caaagtcgcc ggatacgtga agatgatgtc cgaactgtag
     1621 gttttggtcg cttcgagcga aggcgccgta gtcacgattc gctcgaggaa actcgccttc
     1681 gcgacacctt tgtcgtcgta gtagatacca ctgacatcga cgattgcctg gattttgccg
     1741 cccggctgct caccaaaaga gagaaactct ccgggaagtt gaatagccgt tgaaagcgtc
     1801 ggcccctttg gggcgacgtc gtaataatca acgctcatca ggatcggcaa attccgcacg
     1861 ggataaggcg cggcgatcgc ttcgcgtaat ttcgcggtcg gcgttttggt tggcgtcgcc
     1921 gactccgtct tcttgttggc ggcgaccgcc ggcaccggat ccagatcgaa gaaaccttta
     1981 cgaactctga cagtcagatc cggccggccg acgagtttca cttcgagctg tctgaaccgc
     2041 cgttgctttt gcgacaccgc gtccggtttc cacgcgagca ggtaatagga cgaagtctct
     2101 gcgattgcgg gagcaagtcc ttgtgaaagt tcgttggtgt tgaaaatcgc tttgccaccg
     2161 gtgtcagcgg cgagtgcgtg caagccgtcc tgcgacgcgg acagttcacc gaatctcgcg
     2221 ctttgaagtt ggccgctcgg atcgaaagcc tgttcggtac tcgcatcctg cagcgttgcg
     2281 acgagaccgc gagtgtccat tgagtagatc acgacaccgc ttttggcggc agcgttggtg
     2341 atgtcgcgta acttcgtcat cgaatctccg cgccggtttt ccaggaggaa cccgcccgaa
     2401 agaaagaaca ggaccttgcg gccgggcagg tttcgcgcgc cgcggacgag cgattcgagg
     2461 ccgctgagcg tgttaaagtt caggcgtgca gcctgcgttg tgattgactg cgcgcgctgg
     2521 cgaacgatct gttcggccgt atcacggcgg atgccgggat tggttcgaag agtttcgttg
     2581 acaaagaatt cgaacacaga acgatccatg cgctcgatca tcaacgcttc gtactcgctc
     2641 atcggcggcc gatcgaaatc gcgcaccgtg tagctgcgtg gagtgagccg atcaagagcg
     2701 gtatgcaaca ccatccgatc gtgcgtgagt tgttgcagga atccgatctg tccggtagcg
     2761 gacgcgattg ccgcctgatc gttctggccc atctgtttgt caatgaaggt ggagatgacc
     2821 tttttagcgg caagcatgcc cgccaggtcc atgtgaaaat cgtcgagata gaaaaagacg
     2881 atgcggccac gatcgagtgg tacggggcgt tttaagttta cggtcgtggc gccgcgagcc
     2941 gcggcgagtt gcgactcttc gtcgctgccg gcggtgatct tctcgaacgc ctgaattggc
     3001 cggggttttc cgtcaatgcg tagctcgaaa ttttctttag tcagaccgtc aacaaaccgg
     3061 ccctgcttat cgaaaaccat aacgtcggtc tggacgagtt ccgtgtaaac gcgtacaact
     3121 tcgtctgcgg atttggttgg aggcgtctgc gtggtttggc ccgcggcttc gatcgccgtt
     3181 aaaaacgcgc acatcagcgt gagagttgtg caagcgtgac ctttgagaca ggcaaacata
     3241 aagcagggat catgatggca gaaacgggtg taacggtaca actcaaccgc gtcagcaaaa
     3301 gttttggcga ttttaccgcg gtaaaggaac tgagtgtgac ggtgcgcgcg ggacgcgtgt
     3361 ttggactact cggaccgaat ggcgcgggca agactacaac catccgcatg atcgtcaaca
     3421 tcaccgcgcc cgactccggc aagatcgagc tgttcggcgg tcatatcaac tccgcactac
     3481 aggatcggat cggctacctg cccgaagaac gcggtttgta ccgccgtatg aaaatcgtgg
     3541 atcagttacg cttttttgcc gaacttaaaa acgtacgcgg caaagaagtc gaaacgaaga
     3601 tcgaccagtg gctcgcacgc gtgaagcttt ccgagtggaa gaacaagcgt tcgatggaac
     3661 tgtcgaaggg catgcagcag aagatccagt tcgtcaccgc ggtgctgcac gatccggatc
     3721 tgttgattct cgatgagccg ttctccggtc tggatccgat caatcttgag ttgttgaagg
     3781 agatcgtgct cgatctgaaa cgttccggca agacaattat cttctctact caccagatgg
     3841 aagtcgcgga aaaactctgc gacgacatct gcctgctaaa ccgttcgcag aaagtttttg
     3901 aaggcagctt gcgcgagatc aaaagcagct tcggacgcaa cgcagtcgca gtgcgttgtg
     3961 aaggcggcga tggcgtattg gacgatccca cgctcgtaag caaactcgtg cgccatgcag
     4021 acgaagcgca ggcgctgctt gccgagggag cggacgcgca ggtgctcttg cgccggttaa
     4081 tcgactccgg cgccaggatt ggaaagtttg aaatggttga gccgagtctg aacgatatct
     4141 tcatcacgaa ggtaacggaa cacgcatgag aaagtttctc gccgtactca aacgtgaata
     4201 cacacagcgc gttcgcgcca agatgtttat tgtctccacg attctgttgc cgctggtgat
     4261 gtcgctcttt ggcattgtgc cggcggtgat cttgagcatt gaggccgcac ccatgcgagt
     4321 tgccgtgatc gatcagaccg gcaagatcta cgcgcacctg gaagaagctt tggcgagcga
     4381 tgaatcgaag tctgacgaac cggatcaaac tcgaaccggt ccggaagtgg ccggacgtac
     4441 gtttcatttg gaacaagtcg cggcgatcgg tccggaggcg aacgaagagc gagcccgact
     4501 cgaggcgcgt ttgcaaaata aagagctgga cggatacctg gtaataccgg ccgagttcct
     4561 gcaacacgga caagtggagt ttttcaacaa caatcccggc gatgttctta gccggcgcat
     4621 tttgcagtcg gcgttgaacc gcgccgctcg agaggaacga ttggtcgaag cgcgagtcga
     4681 tgcggagacc agacagggat tgtttcagcc ggtgaactta cagtcggtga agatcggtgc
     4741 cgcgggtgaa gagcgcgact ctggtggtag tttcatcctg gtgtttggcg tcg
//
LOCUS       DASTEY010000239         6147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_664326, whole genome shotgun sequence.
ACCESSION   DASTEY010000239 DASTEY010000000
VERSION     DASTEY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664326"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFO72_04280"
     CDS             <1..259
                     /locus_tag="VFO72_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478533.1"
                     /translation="GATKEMTLFTLTRQLIDIPSLTGDEKPAGEFLATHLAGLGYQVE
                     LQHVTTDRFNVIATTGETPRIVFSTHMDTVPPFIASAEDGE"
     gene            260..1018
                     /locus_tag="VFO72_04285"
     CDS             260..1018
                     /locus_tag="VFO72_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9478534.1"
                     /translation="MYGRGSCDAKGIMAAQIFAAEQLRENGTNDIGLLFTFDEELSSA
                     GAQAANQLSLARECRFLINGEPTDNRLATGTKGSLRLFLTTEGRAAHSAYPEAGESAI
                     EKLLDVLEDIRACEWPGDSFFGDTTCNIGVLNGGTRPNVIPDYARAELQIRLATDIER
                     IKEIVERAVAGRAHIEYASAHDPVRLFSVPGFGDCVVRFTTDVPYLSKWGQALLLGPG
                     SILDAHTDHERIAKNELERAVDLYVTLANKLKTL"
     gene            1015..1713
                     /locus_tag="VFO72_04290"
     CDS             1015..1713
                     /locus_tag="VFO72_04290"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET9478535.1"
                     /translation="MKIALIGNGAMGQLVTALATAGGDEVGAVVTSRERDLNPTQLAD
                     KIRGHDVAIDFSVGEAVLKNIEACARAGVPLVEGTTGWKAHEPAAKQLVTEHSAALVY
                     GANFSIGVNLFYRIVDRAAALFASVEGYAPFIEEAHHMRKRDAPSGTALQLRELMAKH
                     LGPDISTASTRAGYIPGTHRVGFDSEADQILLVHEARSRRGFASGALVAAHWVIGKKG
                     VFQFTDVIEEIVDF"
     gene            1822..2745
                     /gene="dapA"
                     /locus_tag="VFO72_04295"
     CDS             1822..2745
                     /gene="dapA"
                     /locus_tag="VFO72_04295"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9478536.1"
                     /translation="MTLKVDWLRGCATALVTPFTNEGAVDDRRLRDLIEYQIAGGVKL
                     LVPCGTTGESVTMSDAENELVIRTTVELARDRAKVIAGTGSNSTALAIQKSRAARSLG
                     ADGILCVAPYYNKPTQEGLYAHFRAIAEAVNSLPVVIYNGPGRTSSNISAETTLRLAR
                     DVENIVGVKEASGNLSQIMEILRGRPENFRVISGDDALTLAMIALGAEGLISVASNEA
                     PALMSQLNEQALAGNWDEARALHYKLLPLMEANFIESSPGPVKAALAMMGLLEEKLRL
                     PLVPVQEKTRARLREILIEIGLLKETSHAVA"
     gene            2732..3565
                     /locus_tag="VFO72_04300"
     CDS             2732..3565
                     /locus_tag="VFO72_04300"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET9478537.1"
                     /translation="MPLREQIESLAARQDGNFTAADRSVFDEFKSALNRGEVRAAERN
                     ANGEWLVHKWVKQGLLLGFRMGALADMSDGESLKFFDKDTYPVRPTKLEDNVRIVPGG
                     STIRDGAYIAPGVVCMPPMYVNAGAYVDEGTMIDSHALVGSCAQIGKRVHISAGAQIG
                     GVLEPVGAVPVIIEDDVLVGGNCGVYEGTIVRERAVVAAGTILTGSTPVYDLVRSEIY
                     QRKTDRSLEIPAGAVVVPGARAVQTEQGRNWGLSLYAAIIVKYRDEKTDTAVQLEDYL
                     R"
     gene            3562..4719
                     /locus_tag="VFO72_04305"
     CDS             3562..4719
                     /locus_tag="VFO72_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9478538.1"
                     /translation="MNNFELPRRLQGIEKSVIRQVFDRAQPGSINLGLGEPDLPTPDV
                     IRRAAVKAIIEEQNGYTSHAGLKSLREKIAAEYPHLEQNPDRVIVTAGSQEALYLALL
                     ALVDDGDEVLLPNPGFVAYPTIVKMAGGTSRFYHLPRENGFAFDADEFRRALTPQTKV
                     VVCISPSNPTGRTLSRDDLIAIADAVRDHGAYLISDEIYRELYYTPERPQSLASFADR
                     TIAIGGLSKSMSMTGWRLGWLGGDEAVVRAALVLHGYVTTCASTVSQKASLVAWSDEA
                     EAAREGFRETFRARRDHLLKLIDTELVLRAVSPDGAFYTMLDVSEFGPSMKVAEALLA
                     ARVITVPGAAFGSESEGFLRVSFCADHDTLAEGVERIKKGLAQSAKAQSKR"
     gene            4802..5863
                     /gene="asd"
                     /locus_tag="VFO72_04310"
     CDS             4802..5863
                     /gene="asd"
                     /locus_tag="VFO72_04310"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET9478539.1"
                     /translation="MSSKFRVGILGATGMVGQRFIQLLAGHPQFEVTAVAASDRSQGR
                     SYGEACTWRLSGEMPAAVRAMPVQPPAPPLDCDFVFSSLPGDIARETEGSFAAAGYPV
                     ISNSSAFRMDEDVPLLIPEVNHEHLGLVERQRERYSKGGFIVTNPNCSTIMLALALAP
                     LHAAFGVEATVATTLQALSGAGYPGVASLDILDNVLPFIGGEEEKIETETTKILGRFT
                     DARIEPAPMAVSAQCHRVNVADGHMAAVRVKLAREATLDNVRDALTSFRSLPQELGLY
                     SAPAAPIVIRDEEDRPQPKFDRDAGQGMSITIGRLKTDSVLDYRFIALSHNTIRGAAG
                     AAILNAELLVALGYLPDHA"
BASE COUNT         1346 a         1653 c         1825 g         1323 t
ORIGIN      
        1 cggtgcgacg aaagagatga cgctttttac acttactcgc caactcatcg acatcccttc
       61 gctgacggga gacgaaaaac ccgccggtga atttctcgcg actcacctcg cgggtcttgg
      121 ctatcaagtg gaactacaac acgtcaccac tgatcgcttc aacgtaattg ctaccaccgg
      181 ggagacgcca cgcatcgtgt tctccactca catggacacg gtgccaccgt tcattgcgtc
      241 agctgaagac ggcgaataaa tctacggccg cggctcgtgc gacgctaaag gaatcatggc
      301 cgcacagata ttcgccgccg aacaactccg cgaaaacggt accaacgata tcggtctgct
      361 cttcacgttt gacgaagaac tgagcagcgc cggcgcacag gcagctaatc aactctcgct
      421 tgctcgcgag tgccgtttcc ttatcaacgg cgaacccact gacaaccgcc tcgccaccgg
      481 aaccaaagga tcgctgcgac tcttcctcac caccgaaggg cgcgcggccc actcggcgta
      541 tcccgaagca ggcgagtcag ccatcgagaa gctgctcgac gttttagagg acatacgcgc
      601 gtgcgaatgg cccggcgaca gtttctttgg cgacaccacg tgcaacatcg gcgtgctgaa
      661 cggcggcacg cgtcccaacg tgattcccga ttatgcccgc gcggaattgc aaattcggct
      721 cgcgacggat atagagcgca ttaaagaaat cgtcgaacgg gccgtcgccg gacgcgcgca
      781 catcgaatac gcgtcggcgc acgatccggt gcgcctgttt tctgttccgg gttttggcga
      841 ttgcgtcgta cggttcacga ccgacgtgcc gtacctgtcg aagtggggac aagcgttgtt
      901 gctcggtccc ggttcgattc tggacgcgca tacggaccac gaacgaatcg ccaaaaacga
      961 actcgagcgg gcggtggatc tatacgtgac actggcaaac aagttgaaga ctttatgaag
     1021 atcgcgctaa tcggaaacgg agcaatgggc caactggtta ctgctctggc aacagccggt
     1081 ggtgacgaag tgggtgcagt ggtgacgtcg agagagaggg atttaaatcc aactcagtta
     1141 gcggacaaga tccgtggtca cgacgtcgcg atcgacttct ccgtaggcga agctgtgctc
     1201 aaaaacatcg aagcatgtgc gcgcgcgggc gttccgctgg tcgagggaac aaccggctgg
     1261 aaggcacacg aaccagccgc aaaacagctc gttaccgaac attcggccgc cctcgtttac
     1321 ggcgcgaact tctcgattgg cgtgaatctg ttttaccgca ttgttgaccg cgccgcggca
     1381 ttgttcgcgt ccgtcgaagg ctacgcacct ttcatcgagg aggcgcacca tatgcgcaaa
     1441 cgcgatgcgc cttcgggaac agccttgcag ttgcgtgagt tgatggcaaa acatctcgga
     1501 ccagacattt ccactgcttc gacgcgtgca ggttacatcc ctggcacgca ccgcgtcggt
     1561 ttcgattccg aggcggatca gatcctgctt gtgcatgaag cgcgatcaag acgcggattc
     1621 gcgtccggag cattggtagc cgcgcattgg gtgatcggaa agaagggcgt ttttcagttt
     1681 acagatgtga ttgaagaaat tgtcgatttt tgattttcga ttgccgattt tcgacttgct
     1741 cgaagctttt tcaatcggca attggcaatc ggcaatcggc aatgtcttca atcgacaatc
     1801 gaaaattcct caggaggcac tatgacacta aaggttgatt ggcttcgtgg ttgtgctacc
     1861 gctctcgtca ctccctttac caacgagggc gccgttgacg acagacgttt gcgcgacctg
     1921 attgaatatc aaattgccgg cggcgtgaaa ctgcttgttc cctgcggcac gacaggcgaa
     1981 agcgtaacga tgtctgacgc agaaaacgaa cttgttattc gcaccaccgt tgaactcgct
     2041 cgcgaccgcg ccaaagtgat cgccggtaca ggcagcaatt ccacggcctt agcaattcag
     2101 aaatcgcgag ccgcgcgatc gcttggagct gacggtattc tctgcgtcgc gccgtattac
     2161 aacaagccga cacaggaagg tctctacgca cactttcgcg ccatcgcgga agccgtgaac
     2221 agtctcccgg ttgtcatcta caacggtccc ggccgcactt cctcgaacat ctcagcggaa
     2281 acaacacttc gcctcgcgcg ggacgttgag aacatcgtcg gcgtcaaaga agcttcgggt
     2341 aacctttcgc agatcatgga aattctgcgt ggtcggccgg aaaatttccg tgtcatctcg
     2401 ggcgacgatg cgctgacact cgcaatgatc gcgctcggag cagaaggttt aatctcagta
     2461 gcctcgaacg aagcaccggc attaatgtcg cagctcaacg aacaggcgct ggctggtaac
     2521 tgggacgaag cgcgcgcgct gcattacaag ttgttaccac tgatggaagc aaactttatc
     2581 gagtcgagtc ctggtccagt gaaggctgcg ctggcaatga tgggcctgct cgaggagaaa
     2641 cttcgcctgc cgctcgtgcc cgtgcaggaa aagactcgcg cgcggttgcg agagatcctc
     2701 atcgagattg gtttactgaa ggaaacttcg catgccgttg cgtgaacaga tcgagtcgct
     2761 cgctgcacgc caggatggaa acttcacggc ggctgaccgg agtgtattcg atgagttcaa
     2821 aagtgcgctc aaccgtggcg aagtacgcgc cgcggaaaga aatgcgaacg gcgaatggct
     2881 ggtccataag tgggtaaagc agggactgct gttgggtttt cgcatgggcg cactggctga
     2941 catgtccgac ggagagagtc ttaagttctt tgacaaagac acgtatccag tgcgaccgac
     3001 aaagctcgag gataacgtgc ggatcgtgcc cggtggatcg acgatacgtg atggcgcgta
     3061 catcgcgccg ggagttgtct gcatgccgcc gatgtatgtg aacgcgggcg cctatgtcga
     3121 cgaaggaacg atgatagaca gtcatgcgct ggtcggatcg tgtgcgcaga ttggcaagcg
     3181 ggtgcacatt tctgccggcg cgcaaatcgg tggcgtgctc gaacccgttg gcgcagtgcc
     3241 ggtgatcatc gaagacgacg tgcttgtcgg tgggaactgt ggtgtttatg aaggcacgat
     3301 cgtccgcgaa cgcgccgtgg ttgcggccgg cacgatcctg acaggttcaa cacccgttta
     3361 cgatctcgtt cgtagtgaaa tctatcaacg aaaaactgat cgttcgctcg aaattcccgc
     3421 cggtgctgta gttgtgccgg gcgcgcgtgc agtgcaaact gaacagggac gcaattgggg
     3481 actgtcgctt tacgcggcaa ttatcgtcaa gtatcgagac gagaagactg acacggcggt
     3541 gcaactcgag gattacctgc gttgaataac tttgagctac cgagacgttt gcagggaatc
     3601 gaaaagagtg tcatccggca agtcttcgat cgcgcccagc ccggcagcat caatctcgga
     3661 ttgggcgaac cggacctgcc gacgcccgac gtgattcgac gtgcagcagt caaagcaata
     3721 atcgaagagc agaacggata cacttcgcac gccggcttga agtctctgcg tgagaagatt
     3781 gccgccgagt atccgcacct cgagcagaat ccggatcgcg tgattgtcac tgctggatcg
     3841 caggaggcgc tgtatctcgc attgctggct ttggttgacg acggcgacga ggtgctgctg
     3901 ccgaacccgg gcttcgtggc gtatccgaca atcgtgaaga tggccggcgg cacatcccgg
     3961 ttctatcacc tgccgcggga gaacggattt gcgtttgatg cagacgagtt tcgccgcgcg
     4021 ttgacgccgc aaacgaaagt cgtcgtttgc atcagcccgt cgaacccgac cggacgcacg
     4081 ctatcgcgag acgatctgat cgcgatcgcg gatgcggtgc gagatcacgg cgcgtatctc
     4141 atcagcgacg agatctatcg cgagctctac tacacgcccg agcgcccgca atctctggct
     4201 tcatttgcag accgcacgat agcgatcggt ggactctcga agtcgatgag catgactggc
     4261 tggcgtctcg gctggttggg tggtgacgaa gccgtcgtgc gcgccgcgct cgtcttacat
     4321 ggttatgtta cgacttgcgc ctcgactgtc tcgcaaaaag cgtcgctggt tgcgtggagc
     4381 gacgaggcgg aagctgcgcg tgagggattt cgcgagacgt ttcgcgcgcg acgcgatcat
     4441 ctgttgaagc tgatcgatac cgagttggta ttgagggcgg ttagtccgga cggtgcgttc
     4501 tacacgatgc tcgacgtaag cgagtttggt ccatcgatga aggtggcgga ggcgttgttg
     4561 gctgcacgcg tgatcacagt gcccggggct gcgtttggca gcgagagtga ggggtttttg
     4621 cgagtgtcgt tctgtgctga ccacgacacg ttggctgagg gagtcgagcg gattaagaaa
     4681 ggactcgcgc aaagcgccaa ggcgcaaagt aagcgctaag cggcctgagt agttcttgtt
     4741 ttcctttgcg tcttggcgcc tcggcgtgag aactgctttg aacaattcga aaggtgtcga
     4801 aatgagtagc aagtttcgag tcgggatctt aggggcgacg gggatggttg ggcagcggtt
     4861 tattcaactg ctcgcgggtc atccacagtt tgaagtaacg gcagtggcgg cgtcggatcg
     4921 atcccaggga aggagttatg gcgaagcgtg tacgtggcgc ttgagtggtg aaatgcctgc
     4981 tgccgtccgc gcgatgccgg tgcagccgcc ggcgccgcca ctcgattgcg atttcgtgtt
     5041 ctcaagttta ccaggcgaca tcgcgcgcga gactgaagga agtttcgccg ccgcaggcta
     5101 tccagtgatt agcaactcct cggcgtttcg catggatgaa gacgtgccgc tgctaattcc
     5161 ggaagtcaat cacgagcatc tcggactagt cgaacgacag cgtgaacgat attcaaaagg
     5221 cggattcatc gttaccaatc caaactgctc gaccatcatg ctcgcgctgg cgcttgcgcc
     5281 gctgcacgcc gcgttcggcg ttgaagcgac cgtggcaacg actcttcagg cgctttccgg
     5341 cgcgggttat cccggcgtcg cgtcgctgga tatcctcgac aatgtgttgc cgttcatcgg
     5401 tggtgaagaa gagaagattg aaactgaaac cacaaagata ctcgggcgtt tcactgacgc
     5461 gcggatcgaa cccgcgccaa tggcggttag tgctcagtgc catcgcgtga atgttgccga
     5521 cggacacatg gctgcggtgc gcgtgaagct tgcacgcgaa gcgacgctgg acaacgttcg
     5581 cgacgcgttg acgtctttcc gttcgttgcc acaggagctt gggctatatt ctgcgccggc
     5641 cgcgccgatt gtcatacgtg acgaggagga ccggccgcaa cccaagtttg atcgtgacgc
     5701 cgggcaggga atgtccataa ctatcggccg gctaaaaacc gactcagttt tggactatcg
     5761 atttatagct ttgagccaca acaccatccg cggtgcggca ggtgctgcta ttttgaatgc
     5821 cgaactgctc gtcgctttgg gctacttacc tgaccacgca tgaaaaccaa cctaagtttc
     5881 gttgcgctgt tactttgcgt tctaacttct gcggcacagg ctggtcgaag ggttagggaa
     5941 aaggtagatt catacaaatt tctatagacg ccaccagaaa tttggatata ctttgcctca
     6001 caaaaccgca aggtctttac ccgaacgtga aaatgcgagt gtttttgtgg cggagtgagc
     6061 actgcgcttc ccggaatagt atctgcaaga aatctgattg attcgacgag tttttcaacg
     6121 ttaaatccgc ctctcatctt atcggat
//
LOCUS       DASTEY010000240         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1093219, whole genome shotgun sequence.
ACCESSION   DASTEY010000240 DASTEY010000000
VERSION     DASTEY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093219"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..952)
                     /gene="kdsA"
                     /locus_tag="VFO72_04315"
     CDS             complement(116..952)
                     /gene="kdsA"
                     /locus_tag="VFO72_04315"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET9478540.1"
                     /translation="MTGVKAFSVGKATFGDGRLTIIAGPCVIESPDHAMMMARECSQR
                     ARAAGLDYVFKASFDKANRSSIHSFRGLGMEAGLDALQRIKEEFGVPVLTDVHDVSQV
                     EAVAEVADVLQIPAFLCRQTDLIVAAAKSGRAVNVKKGQFLAPQDARNIVDKVREAGC
                     EKLLLTERGASFGYNNLVVDMRSLPIMRSFGVPVVFDVTHSLQLPGGLGHATSGQSEY
                     IEPLARAGVACGVDAVFMEVHDNPEKALSDGPNALPLHRLEPLLFMIRDIHELVRNAT
                     RD"
     gene            complement(952..1992)
                     /locus_tag="VFO72_04320"
     CDS             complement(952..1992)
                     /locus_tag="VFO72_04320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9478541.1"
                     /translation="MLNNGSLTYQTLDEKYYLEERLGEGGMGTVYRARHLLMDRPVAI
                     KVLHPRFVENEAARTRFQLEARAAVLLHHANAVSVTDFGQTQEGLVYLVMELLRGRTL
                     REILTKEAPLETARATSIMLQASAAVAAAHDAGIIHRDLKPSNILVTRSADAPAVVKV
                     LDFGIAKLAADVLEGEGASAVTLPGSPVGTPRYMSPEQYEGRELTPAADVYSLGVILY
                     EMLTGVAPFTGVSPVEIALKHANNAPLPPRAIVAAIPEDVERVVLHALEKQPTARPEN
                     AAEFRRELLEAAERLGLEHQAITSAPDIKALRGAALESPSGRLVVDIAKLRETRATDK
                     TDKNGSRILPSD"
     gene            complement(2199..>3122)
                     /locus_tag="VFO72_04325"
     CDS             complement(2199..>3122)
                     /locus_tag="VFO72_04325"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9478542.1"
                     /translation="DLIVMQLGLHGQAPQADLSEWRDLVATIREPSGGEASIAIVGKY
                     VELEDSYKSLREALTHGGVANNLRVDVKWIESEELMDDDYEDRLRDFDAILVPGGFGK
                     RGVAGMIRAIYYARKTRTPFFGICLGMQTACLEYARNVCELKDADSTEFDPDTPHPII
                     FKLRDLVGVEELGGTMRLGAWLCRLAPGSLVRDIYCGAEEVSERHRHRYEFNPEYRPR
                     LERAGLVFSGVSPDGKFVEMIELPREVHPWFVGCQFHPEYKSKPLNAHPLFAAFVRAA
                     YENRLQSETSMEKNGEVDLVMHERVSATSGD"
BASE COUNT          691 a          962 c          821 g          648 t
ORIGIN      
        1 aaacgaattt acctgccaga tgacagacgc cgtttgcgcg ctggcgtgca cgcagacgaa
       61 cagcaatagc atcaacgaaa gaaacgtttt cataatgaag agaaactaat gtggattaat
      121 ctcgcgtcgc gtttcgcacc agctcgtgga tgtcgcgaat catgaacagc agcggctcga
      181 ggcgatgcaa cggcaacgcg ttcggaccat ctgacaaggc cttctccggg ttgtcgtgaa
      241 cttccataaa aaccgcatcc actccgcaag ccacacctgc gcgcgcgagg ggttcgatgt
      301 attccgattg accgctggtt gcatgtccaa gcccgcccgg cagttgcagc gaatgcgtca
      361 cgtcaaacac gaccggcacg ccaaagctcc gcatgatcgg taacgaacgc atgtcaacga
      421 ccagattgtt ataaccaaaa ctcgcgccgc gctccgtcaa caaaagcttt tcacaaccag
      481 cttcacgaac tttgtcgacg atattccgtg catcctgtgg cgcgagaaac tgtccttttt
      541 taacgttgac tgccctgccc gacttcgccg ccgccacgat cagatcggtt tgccggcaaa
      601 ggaacgcggg aatctgcaac acatcggcga cctccgccac tgcttcgact tgcgaaacgt
      661 cgtgcacgtc ggtgagcacc ggaacgccga actcttcctt gatacgttgg agcgcgtcga
      721 gtcccgcttc cattcccaag ccacgaaagc tatggatgga cgagcggttt gccttgtcaa
      781 acgaagcctt gaaaacgtaa tccaggccag ccgctcgcgc gcgctgcgaa cactcgcgcg
      841 ccatcatcat cgcgtggtcc ggactctcga tcacacatgg tccggcaatg atcgtcagcc
      901 gcccgtcacc aaacgtagct ttgccaacac tgaaagcttt aacaccagtc attaatctga
      961 ggggagtata cgcgatccgt tcttgtccgt cttatccgtg gcccttgttt cgcgcagctt
     1021 cgcgatatcc acgaccaacc ggcccgacgg cgattccaac gccgcgccac gcaacgcttt
     1081 gatgtccggc gcactagtga ttgcctgatg ttccaaccca agcctctccg cggcttcgag
     1141 tagttcacgg cgaaattcag ccgcgttttc aggccgtgcg gtaggttgtt tctcgagtgc
     1201 gtgcaagacg acgcgttcca cgtcctcagg aatcgccgcc acgatcgcgc gcggcggcaa
     1261 aggcgcattg ttcgcgtgtt tgagcgcgat ctccaccggc gacactccgg taaaaggcgc
     1321 aacgccagtc aacatctcgt agagaatcac tccgagactg tagacgtccg ctgccggcgt
     1381 tagctcgcgt ccttcgtatt gttcgggcga catgtagcgc ggcgtgccga cgggactgcc
     1441 cggcaacgtc acagcactcg cgccttcacc ttccagaacg tcagcggcca gttttgcaat
     1501 accaaaatcg agcactttca cgaccgcggg agcatccgca ctccgcgtca ccaaaatgtt
     1561 cgacggcttc agatcgcgat gaatgattcc ggcatcatgc gccgcggcga ccgcagccga
     1621 cgcttgcaac atgatcgaag tcgcgcgcgc ggtttcgagt ggcgcttctt tggtcaggat
     1681 ctcgcgcaac gtgcggcccc gcagcaactc catcacgagg tagaccagac cttcctgcgt
     1741 ctggccgaag tcggtcaccg acactgcgtt tgcgtgatgc agcaacacgg ctgcgcgcgc
     1801 ttccagttga aaacgcgtgc gggcggcttc gttctcgaca aagcgtggat gcaacacctt
     1861 gatcgccacc ggacggtcca tcagcagatg gcgcgcgcga tagaccgtgc ccatgccgcc
     1921 ttcgccgaga cgttcttcga ggtagtattt ctcgtcgaga gtttgatatg tgagtgagcc
     1981 gttatttagc aatgctttgc ctcaagagtc cctctctcat tctcaccggg ctttagcccg
     2041 atgcaagagc agtgaagacg gagaaaccgt ttcaacggtt tttttcgtgg attcgccagc
     2101 gaaaccgttg aaacggtttc tcccaaccgc gtgccgttac accgggctga agcccggtga
     2161 gaatgagagc ggacttttga ggcaaagcca tttagcaatc aatccccact ggtggcactt
     2221 acacgttcgt gcatcaccag atcgacctcg ccattcttct ccatcgaggt ctctgactgc
     2281 aaacgatttt cgtaagcggc gcgaacaaac gctgcaaaca acgggtgggc attgagcggt
     2341 ttcgatttgt attccgggtg aaactggcaa ccaacaaacc acggatgcac ttcgcgcggg
     2401 agttcgatca tctcgacaaa cttgccgtcg ggcgagacgc cactaaacac gagtcctgcg
     2461 cgttccagcc gcggccggta ctccggattg aattcgtatc gatgacgatg acgttcgctg
     2521 acctcttcgg cgccgcaata gatgtcgcgc acgagtgaac caggagcgag ccggcagagc
     2581 cacgcgccaa gtcgcatcgt gccacccaac tcttccacgc caacgaggtc gcgcaatttg
     2641 aagatgatcg gatgcggcgt atcgggatcg aactcggttg aatcagcgtc tttcagttca
     2701 caaacgttac gcgcgtactc gagacacgcg gtctgcatac caagacagat accaaaaaac
     2761 ggtgtgcgag ttttgcgcgc gtagtagatc gcgcgaatca ttcccgcgac gccccgtttg
     2821 ccaaagccac cgggaacaag gatcgcgtcg aagtcacgaa gacgatcttc atagtcgtcg
     2881 tccatcaact cttccgactc gatccactta acgtcgacgc gcaaattgtt tgcgacgccg
     2941 ccgtgtgtca gcgcttcgcg taacgacttg tacgaatctt caagctctac gtacttaccg
     3001 acaattgcta tcgatgcttc accgcccgaa ggctcgcgga tcgtggccac cagatcgcgc
     3061 cactccgaca gatctgcttg cggcgcctgt ccatgcaaac ccaattgcat gacgatcaga
     3121 tc
//
LOCUS       DASTEY010000241         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_318308, whole genome shotgun sequence.
ACCESSION   DASTEY010000241 DASTEY010000000
VERSION     DASTEY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318308"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFO72_04330"
     CDS             <1..220
                     /locus_tag="VFO72_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478543.1"
                     /translation="VKLGEMATAIELLTAAQALDYRAPLAPGRGVKEAHRIVRSLVEP
                     LTTDRSMAGDIEKLSAAIIRGEFEELLG"
     gene            363..656
                     /locus_tag="VFO72_04335"
     CDS             363..656
                     /locus_tag="VFO72_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET9478544.1"
                     /translation="MDERRKGLEEEYFHRKNKEAIEKLRAKMKVAEEAKAAGISSMQC
                     PRCDGSLKESKFEEVLIDTCEKCGGVWLDSGELEQLTQKDKGGWFGRLWQAKD"
     gene            668..>2103
                     /gene="hutU"
                     /locus_tag="VFO72_04340"
     CDS             668..>2103
                     /gene="hutU"
                     /locus_tag="VFO72_04340"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET9478545.1"
                     /translation="MHTDEHKVIRAPRGAQLSCKGWHQEAALRCLMNNLDPDVAEKPD
                     ELIVYGGAGKAARNWESFAALVRELRTLENDETLLVQSGKPVGVFRTHEYAPRVLIAN
                     SNLVGAWATWEHFHELERRGLMMYGQMTAGSWIYIGTQGIVQGTFETFAAMADKHFGG
                     DLSGKLVVSGGMGGMGGAQPLAATLNGAVFIGIEVDPARIERRVSTGYCDRIAMTLDE
                     ALDICEDAREQKRAISVGLVGNCAEILPEIVRRGVKVDAVTDQTSAHDPLNGYVPAGL
                     PLDEAAALREKDPDGYVERSKASMAQHVSAMLALQRSGAVVFDYGNNIRKQAFDAGCK
                     DAFEIPGFVPEYIRPLFCEGKGPFRWVALSGDAEDIRKTDDLALEMFPDDRTLNRWLR
                     LARDRVRFQGLPARICWLGYGERAEMGEAINDLVKRGDLKAPIAIGRDHLDTGSVASP
                     FRETEGMKDGSDAVADWPILNALLNTASG"
BASE COUNT          474 a          542 c          677 g          410 t
ORIGIN      
        1 cgtcaagctt ggagagatgg cgacggcgat cgaactactg accgcggcac aggcgctcga
       61 ctaccgggcg ccgctcgcgc ccggacgcgg agtaaaagaa gcgcatcgga tcgttcgcag
      121 tttggtcgag cctttaacca cagatcgctc gatggccggc gatatcgaga agctcagcgc
      181 ggcgatcatt cgaggcgagt ttgaagagtt gttagggtag ttctcgcgca aaggcgcaaa
      241 ggcgcaaagg aaaaaggcaa aggaaaaaac cagctaaggg gtaatgtggg tggtttttct
      301 tgcgcctttg cgcctttgcg cgagaaaagt ttttgaactt ctaaggagca aatatgtcgg
      361 gaatggacga acggagaaaa ggacttgaag aagagtactt tcatcgcaag aacaaggaag
      421 caattgagaa gctgcgcgca aagatgaaag tcgccgaaga ggcgaaggcc gcgggcatat
      481 cgtcaatgca gtgtccgcgc tgtgatggca gtttgaaaga gagcaagttt gaagaggttt
      541 tgatcgacac ctgcgaaaag tgtggcggcg tgtggctcga ctcgggcgag ctcgagcagt
      601 tgacgcagaa ggacaaaggc ggatggttcg gtcgtctttg gcaagctaaa gattaatggc
      661 gcggcggatg cacacggatg aacacaaagt gatccgcgct ccgcgtggcg cccagttgag
      721 ctgtaaaggg tggcatcagg aagcggcgct gcgctgcctg atgaacaatc tcgatccgga
      781 cgttgccgaa aaaccggatg aacttatcgt ttacggtggc gccggcaagg ccgctcgcaa
      841 ctgggaatct ttcgctgcac tcgtacgcga actgcgcacg ctcgaaaacg acgaaacgct
      901 gttggtccag tccggcaaac cggtcggcgt gtttcgcacg cacgaatacg caccacgcgt
      961 tttgatcgcc aactcgaacc tcgtcggcgc ctgggccacc tgggaacatt tccacgaact
     1021 cgaacgtcgc ggtctgatga tgtacggcca gatgacggcc ggcagttgga tctacatcgg
     1081 gacgcagggc atcgttcaag gaacgtttga aacgtttgcc gcgatggccg acaaacattt
     1141 tggcggtgat ctgtccggca agctggttgt ttccggaggg atgggtggca tgggcggcgc
     1201 acagccgttg gccgcgacct tgaacggcgc ggtttttatc gggattgagg tcgaccccgc
     1261 acgcatcgag cggcgcgtca gtacgggcta ctgcgatcgg atcgctatga ctctcgacga
     1321 agcgctcgac atctgcgagg acgcgcgtga acaaaaacgt gccatttcag ttggtctagt
     1381 tggcaactgt gcggagatct tgccggagat tgtgcgtcgt ggcgtgaagg tcgatgcagt
     1441 gaccgatcaa accagcgcgc acgatccgtt gaatggttac gtgcctgccg gactgccact
     1501 cgacgaggcc gcggcgctgc gtgaaaaaga tcccgacggc tatgtggaaa gatcgaaggc
     1561 gtcgatggcg cagcacgtca gcgcgatgct cgcattgcaa cggtccggcg cggtcgtttt
     1621 tgactatggc aacaacattc gcaagcaagc gtttgacgcg ggctgcaaag acgcgttcga
     1681 aatacccggc ttcgtgcccg aatacatccg cccgctcttc tgtgaaggca aaggaccatt
     1741 ccgctgggtc gcgctttcag gcgacgcgga agacattcgc aagactgacg acctcgccct
     1801 cgagatgttt cctgacgata gaaccttgaa tcgctggttg cgtcttgcgc gcgatcgcgt
     1861 gcggttccag ggtctgccgg cgcgcatctg ctggctcggg tacggcgaac gcgccgagat
     1921 gggcgaagct atcaacgatc tcgtcaaacg aggcgacttg aaggcgccga tcgcgatcgg
     1981 gcgcgatcat ctcgacaccg gttccgttgc gtcgccgttt cgcgaaactg aaggaatgaa
     2041 agacggatcg gatgcagtcg ctgactggcc gatcctgaat gctttgctga acactgcgag
     2101 tgg
//
LOCUS       DASTEY010000242         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_417321, whole genome shotgun sequence.
ACCESSION   DASTEY010000242 DASTEY010000000
VERSION     DASTEY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417321"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="argE"
                     /locus_tag="VFO72_04345"
     CDS             complement(<1..999)
                     /gene="argE"
                     /locus_tag="VFO72_04345"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET9478546.1"
                     /translation="MKVTETLAELVQIDSVSSHSNAEIVDYLARRCDALKLKTRRYSY
                     RDEHGVEKINLIALAGADFTQDTAVELVLVGHTDTVPYDPSWSEATNLTERNGKLYGR
                     GSCDTKGFIAAALTAVETLDLSRLNKPLALIFTADEEIGLRGAKHLAQLKPLRVRYSI
                     VGEPTSLQPIRAGKGYSLAEVVVKGREAHSAYPALGASAVFRAARLINHLESIAEQLK
                     EDQHPAFDPPYTTLNVGMIHGGSAKNVLAGECRFTLEWRPVPTQPSDHLLHLFSLAIS
                     EEKRADPDFACEVDASRADTGFETAPESPLIRLLENETGLDAGTVAFGTEGAQMQAL"
     gene            complement(996..>2268)
                     /locus_tag="VFO72_04350"
     CDS             complement(996..>2268)
                     /locus_tag="VFO72_04350"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET9478547.1"
                     /translation="LRPAQNVADPIRLKNRALLPGLINAHSHAFQRVIRGRTERRSQS
                     TTDTFWTWRQQMYAAANRLAPEDIYAVSRMAFLEMALSGITAVGEFHYIHHAPDGSRY
                     SDPNMVEREVIRAARDVGLRIALMRVAYARAGFQTEPNPLQVRFIENSPEIYLANLEQ
                     LLAATELKDGMAWIGVAPHSVRAVPLDYLKSIVEFANARKLVVHMHVAEQTAEVEACI
                     EEYGRSPVALLDTEGLLNEKFTAVHAIHVTPKAVSALAQSKANVCACPTTERNLGDGV
                     VPADAYFNKGVPISLGTDSQTQIHLLEDARELEYHLRLQRSARNVLAPADDVDESALA
                     RRLFDCATVNGAISIGFDGGNLEPGAPADFFTVDLSDPSIAGSSPDNLLANIVFSLSR
                     TAIRDVVVGGKRIVEDGRHRDQEDIVTAFRQ"
BASE COUNT          465 a          646 c          664 g          493 t
ORIGIN      
        1 aagcgcctgc atttgagcgc cttcagtgcc gaaggcgacg gtgccggcgt cgagtccggt
       61 ctcgttttcg agcagtctaa tcagcggcga ctcaggcgca gtctcaaacc ccgtatccgc
      121 acggctggca tctacttcgc acgcgaaatc gggatcggcg cgtttctctt cgctgattgc
      181 caggctaaag agatgcaaca gatggtctga cggttgggtg ggtaccggtc gccattcgag
      241 tgtgaaacgg cactcaccgg caagcacgtt cttggcggag ccgccgtgaa tcataccgac
      301 attgagcgtc gtgtaaggcg ggtcgaaggc gggatgctgg tcctctttca gttgttcggc
      361 aatgctttcg aggtggttga tgagccgcgc ggcgcgaaac accgccgacg ccccgagtgc
      421 gggatacgcg ctgtgcgcct cacgtccttt cactacgact tcggcgagtg agtaaccttt
      481 tccggcgcgg atcggttgca gcgaagttgg ctcgccgacg atgctgtaac gcacacgcaa
      541 cggtttgagt tgggcgaggt gcttggcgcc gcgcaaaccg atttcttcgt cagccgtgaa
      601 tatcaaagcg agtggtttgt ttaagcgtga gagatcgagc gtttccacgg cggtgagtgc
      661 ggcagcgatg aagccttttg tgtcgcacga gccgcggcca tacagtttgc cattacgctc
      721 agtaagattt gtggcttcgg accagcttgg gtcgtaagga acggtatctg tgtggcccac
      781 gagcacgagt tcgaccgctg tgtcttgcgt gaagtcggcg ccggcgagtg cgatgagatt
      841 gatcttctca acgccgtgct cgtcgcgata cgagtagcgc ctggttttga gtttgagtgc
      901 gtcacaccga cgggcaaggt agtcaacgat ttccgcattc gaatgtgacg aaacggaatc
      961 gatctgcaca agttcagcta aagtttccgt aactttcatt gtctaaacgc agtgacaata
     1021 tcttcctggt ctctgtggcg gccgtcttcg acgatccttt tcccacctac tacgacgtcc
     1081 ctgatcgccg tgcgcgaaag cgaaaagacg atgttcgcca aaagattgtc cggtgacgaa
     1141 ccagcgattg atgggtcgct gagatcgacg gtgaaaaagt ccgccggcgc tcccggctcc
     1201 aggtttccac cgtcgaagcc gatacttatc gcaccgttga cggtagcaca atcgaacaat
     1261 cgccgcgcta gagctgattc gtccacgtca tcggccggcg ccagaacgtt tcgcgcggag
     1321 cgctggagac gcaagtggta ctcgagttcg cgcgcatctt ccagaaggtg gatctgcgtc
     1381 tggctgtcgg tgcccaacga aatcggcacg cccttgttga aataggcgtc agcgggcacc
     1441 acgccgtcac cgagattacg ctcagtcgtc ggacacgcgc acacgttcgc cttgctttgc
     1501 gccagagcgc tgaccgcttt cggcgtcacg tgaatcgcgt gcacggcggt gaacttctca
     1561 ttcagcaaac cttcagtatc gagtagcgcg accggcgaac gtccgtactc ttcaatgcac
     1621 gcctcaactt ctgccgtctg ttcggccacg tgcatgtgaa ccacgagctt ccgggcattc
     1681 gcaaactcaa cgatactttt taggtaatcg agcggcactg ctcgcacgct atgcggcgcc
     1741 acaccaatcc acgccatccc gtccttcaac tccgtcgcgg cgagcaattg ttcaaggttc
     1801 gccaggtaaa tctccggcga gttttcaata aagcgaactt gaagtggatt gggttcagtt
     1861 tgaaatcctg cgcgcgcgta agccacgcgc ataagcgcaa tgcgcaatcc gacgtcgcga
     1921 gccgcacgga tcacttcgcg ttcgaccatg ttcggatccg agtaacgcga accatcgggc
     1981 gcgtggtgaa tgtagtgaaa ctcgccgacc gccgtgatgc cagagagcgc catctcgaga
     2041 aatgccatac gcgacacggc gtaaatatct tccggcgcca gccgattggc agccgcatac
     2101 atctgctggc gccacgtcca aaacgtatcg gtcgtacttt gcgaacgccg ttcggtccga
     2161 ccccgaatga cacgttgaaa cgcgtgtgag tgcgcgttga tgagtcccgg caatagagca
     2221 cggtttttga gacgaattgg atcggcaacg ttttgagcag gacgcaac
//
LOCUS       DASTEY010000243         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1603770, whole genome shotgun sequence.
ACCESSION   DASTEY010000243 DASTEY010000000
VERSION     DASTEY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603770"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..1803
                     /gene="rho"
                     /locus_tag="VFO72_04355"
     CDS             418..1803
                     /gene="rho"
                     /locus_tag="VFO72_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET9478548.1"
                     /translation="MSQRTRSRASSANNEANGDDGGATVATAEAPERETRDGRDGKNE
                     GFHNLADLKEMSISKLTQIAKSMDVPGATGMRKQELIFKVLAAQTEKSGLIFSEGVLE
                     TLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQIRPPKEGERYFALI
                     KVEAINFESPDQAREKVFFDNLTPLYPEEQLRMETTPDNLSARVIDLVTPLGKGQRGL
                     IVAPPRTGKTMLLQTIANSVTQNHPETTLIVLLIDERPEEVTDMQRSVNGEVISSTFD
                     EPPTRHVQVADMVIEKAKRLVEHKRDVVILLDSITRLARAHNAVVPPSGKILSGGIDS
                     NALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFKGTGNSEIHLDRRL
                     SDKRLFPAIDLQRSGTRKEELLISKEDLNRIWVMRRVLNPLSPVEQMEVVLERLEKTK
                     SNAEFLASMQS"
     gene            1883..>2501
                     /locus_tag="VFO72_04360"
     CDS             1883..>2501
                     /locus_tag="VFO72_04360"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET9478549.1"
                     /translation="MKIAILTSEAMPFAKTGGLADVSGALPKALGEHEVDARLFLPLY
                     DQIDRALLNGGSVENVRVEWRGQVHHVRFWYSDAAGAPTYLLEAPEFFARPNIYGYHD
                     DHIRFAFFSRAALALLKSIDWQPDIVHGNDWPCGFAVAELRARRRHDAFFQNTRTLFS
                     LHNVGYQGLFDAGDLWWLGFGEPPERDDFMLDRAASALKAGIIAAD"
BASE COUNT          585 a          704 c          678 g          534 t
ORIGIN      
        1 ctcggccaaa agcggccgcg gtcgcaacga gctttggcgt gcaatagaga gcgcaatcga
       61 gcggagctaa attgacatct ctatgcaaca gagaagaaat tctcgtttgc gcttttcaac
      121 agtaagcttg tatcctgaaa cgaaagtcgg caatccgaaa ttctcatcag ccccctaggt
      181 caacctcagt ttttgggcca cgcgcgtagc acgggtggca tcaaaaacga tactgaattt
      241 gccgcgtacg aaaagttgag gtacagtgca taacaccgtt tgaggagaat cttattgccc
      301 cgcgaatgaa gtgggaactt tcccacgtct ctcgctctac tgcatcattg ttcataatcc
      361 cactagttga taatcgccca taacgagtaa agaaatatct tgccgagaaa aactgatatg
      421 tcacaacgaa cacgttcacg cgcttcgagc gcgaacaacg aagccaacgg agacgacggc
      481 ggcgccacgg tagcaaccgc cgaagctccc gaacgagaaa ccagagacgg ccgcgacggc
      541 aagaacgaag gttttcacaa tcttgccgat ctcaaggaga tgtcgatctc caagctgacg
      601 cagattgcga aaagcatgga cgtgcccggc gctacgggca tgcgtaagca ggagctcatc
      661 ttcaaagtgc tggccgcgca gaccgagaaa agcggcttga tcttttccga gggcgtgctg
      721 gaaacgctgc ccgacggctt cgggtttctc cgcgctcctg agtacaacta tctgcccggt
      781 ccggacgaca tctatgtctc cccgtcgcag attcgcaagt ttgacctgcg caccggcgac
      841 accgtcagcg gccagatccg tccgccgaag gaaggcgaac gctatttcgc gttaatcaaa
      901 gtcgaagcaa tcaactttga gtcgccagat caggcacgag aaaaggtctt cttcgacaac
      961 ctgacgccgc tctatccgga agagcaactg aggatggaga ccacgcccga taacctctcc
     1021 gcgcgcgtca tcgacctggt gacgccgctt ggaaaagggc agcgtggttt gattgtcgcg
     1081 ccgccgcgta ccggtaaaac gatgctgctg caaacgatcg ccaactccgt tacgcagaac
     1141 catccggaaa cgacactgat cgtcctgctc atcgacgaac gaccggaaga agtcactgac
     1201 atgcagcgat cggtgaacgg cgaagtcatc tcgtcgacgt ttgacgaacc accgacacgg
     1261 cacgtccagg tcgccgacat ggtgatcgag aaagcgaaac gactcgtcga acacaaacgc
     1321 gacgtagtca ttctgctcga ctcgattaca cgtctggcgc gcgcgcacaa cgccgtcgtt
     1381 ccgccgagcg gcaagattct ctctggcggt attgattcca acgcgttgca gcgtcctaag
     1441 cgtttcttcg gcgcggcccg taacatcgaa gaaggtggat cgttgaccat cattgccacc
     1501 gcgctaatcg ataccggttc gcgcatggac gacgtgatct ttgaagagtt caaaggcacc
     1561 ggcaactctg aaatccacct cgaccgccgt ttgagcgaca agcgactgtt cccggcaatc
     1621 gatctgcaac gttccggaac ccgcaaagaa gagttgctca tcagtaaaga agatctcaat
     1681 cgcatttggg tgatgaggcg tgtgttgaac ccgctctcgc ctgtcgagca gatggaagtc
     1741 gtgctcgaac gattagagaa aacgaaatcg aacgccgaat ttctcgcgtc gatgcagagc
     1801 taagccacag acgaaaggat ggacacttat ctgtgttcat ccttttcttc tgtctcccac
     1861 ccttagtttc ctccgttcat agatgaagat cgcgattctg acttctgaag ctatgccctt
     1921 tgccaagacc ggcgggctgg ctgatgtttc cggagcattg ccgaaagcac ttggcgaaca
     1981 cgaggtcgat gcgcgcctgt ttctaccgct ctacgatcag atcgatcgcg cgttgctgaa
     2041 cggaggctct gttgagaacg ttcgcgtcga gtggcgcgga caagttcatc acgttcgttt
     2101 ctggtatagc gatgctgcgg gagcgcccac atatctgctg gaggcgcctg agttttttgc
     2161 gcgcccgaac atttacggct atcacgacga ccatattcgg tttgccttct tctcgcgcgc
     2221 ggcactcgcg ctgctgaagt cgatcgactg gcagccggat atcgtccacg gtaacgattg
     2281 gccgtgcggg tttgcagtcg ctgaattgcg cgctcgtcgc cgtcatgatg cgttcttcca
     2341 aaacaccaga acgctgttct cgttgcataa cgtcggttac cagggattgt tcgatgctgg
     2401 cgatctttgg tggctggggt ttggcgagcc gcccgaacgt gacgacttca tgctcgatcg
     2461 tgctgcttcg gcgctgaaag ctggaatcat cgcggccgat g
//
LOCUS       DASTEY010000244         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_450192, whole genome shotgun sequence.
ACCESSION   DASTEY010000244 DASTEY010000000
VERSION     DASTEY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450192"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1026)
                     /locus_tag="VFO72_04365"
     CDS             complement(94..1026)
                     /locus_tag="VFO72_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478550.1"
                     /translation="MLQFPNGWLAFELGALRRLKFNSIALPFTGEPELAVHLKRSKVR
                     VAANDPMIWSHTKALAVIENNTERLDEEDLQTLLDDVYVPREKLNNPSLLKWFNEADA
                     WWFDNLNFNAQQLDLYKRALALTAGMMTGDYVLSFNEETRALRKPLSLSDTFRRLLAC
                     LPEPQDNGMRNQSTHQDVRSFVAERHHTDLLFLRLPVPVTQTPITRDPLVTWREEWLR
                     GSDDFWLDFERARQSRLGSPVQSKQQYLGFVEDLLRTASHIPTWAIAHTENGFISNDE
                     LVEKISRVRKVGSIYSKDFSDMLGVRASVIVSKA"
     gene            complement(1114..1818)
                     /locus_tag="VFO72_04370"
     CDS             complement(1114..1818)
                     /locus_tag="VFO72_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9478551.1"
                     /translation="MKLDAMVAFQQTDLIDKTTDEAFSEIAKTTDRFERYDHRHAQRI
                     AIIADELAMLFHLARHDRGSLHAAALLHDLGEVAMERDYIQVERPLTTEERIDLARHP
                     IIGEREASRVGADRAAQLLVRWHHEWWNGAGYPDALRREEIPLAARILRVADSYAALT
                     DARPFRAALSEAETRRELADRAGIEFDPAVVNVFLSLEPLPEMASFAGTGEDEPVSES
                     ETKPGRRWRLFSSFSK"
     gene            complement(1818..>3492)
                     /locus_tag="VFO72_04375"
     CDS             complement(1818..>3492)
                     /locus_tag="VFO72_04375"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9478552.1"
                     /translation="CVAQRSTPPLDQPIKNGGAPEGRQTRRRAREQLTLTAKNLETVS
                     TVFVYDVIRMNGQLSEQPLAELIREISAKSLAGKLQLQHDRVKIVIYFDKGELVYATS
                     NVRTLRLREYLLKAGIAEAALARYDQRRPDIELARTLTADHLLSPAAAQQIQTKQVTD
                     TLRLALSWTEGAWEFDPRSRLDEKLNIDIDTSALLLDTGRRLPPKFAASRFRNSAELI
                     SPASSPPESTNLLPTEVFLLSRLDRPTPLNELFAVSGISENETYVIVYSLVLAGLLER
                     EHWSDVFRRQPAAAQTLPATQENPPQPAPPTPEKPADEQDEAKSFLEQLNSAETFYDV
                     LGVSKESSPAQMKDKYYELARRYHPDRFRKAEPSLLARLETAFARIAQAYDTLRDDNL
                     RANYDAKLAARRKAQQLADATAKPTTTPTTPQGAAPANPSEPAISVAERAELHFKEGL
                     EALELGQRKAAVGLFASAANAVPKEARYRAFYGRLLAEHEHTRRAAEAELQAALKLDP
                     TNGEYRVMLAELYRDLGLMLRARGEAERAIASDPNNQKARDLLRALKSV"
BASE COUNT          685 a          908 c         1073 g          826 t
ORIGIN      
        1 taccgcgttt cttaacggct ctctttgcgc ctttgcgcga gaataattct tcccataaag
       61 atccgctgag tactttcggg caagcgggtt gaactatgcc ttcgagacga tcacagaggc
      121 acgtacgcca agcatgtctg aaaagtcttt ggagtagatc gaccccacct tccgcacgcg
      181 gctaatcttc tcaactaact catcgttcga aataaaacca ttctccgtat gggcgatggc
      241 ccatgtcgga atgtgcgaag ccgtgcgcaa gaggtcttcg acgaagccga ggtactgctg
      301 tttgctttgt acaggcgaac cgagccgtga ctgtcgtgct cgttcgaagt cgagccagaa
      361 gtcgtcgctg ccacgcagcc attcttcgcg ccaggtaact agcggatctc gggttatcgg
      421 ggtttgagta acgggaacgg gcaaacgcag aaagagcagg tcggtgtggt gtcgttcggc
      481 gacgaagctg cgcacatcct gatgtgtgct ttggttgcgc atgccgttgt cctgtggttc
      541 ggggaggcaa gcgagcagtc gccgaaaggt atcggagagt gaaagaggtt tacgcaacgc
      601 gcgtgtctct tcgttaaaag aaagaacgta atcacccgtc atcatgccgg cggtcagcgc
      661 cagcgcgcgt ttatagaggt cgagctgttg cgcgttgaag ttgagattgt cgaaccacca
      721 ggcgtcggct tcattgaacc acttgagtaa tgatggattg ttgagctttt cgcgcggaac
      781 gtaaacgtca tcgagcagcg tctggagatc ttcttcgtcg agtcgttcag tattgttttc
      841 gatgacggcg agcgctttgg tgtgcgacca gatcatcgga tcgtttgccg ccacgcgcac
      901 tttggatcgt ttgagatgta cggcgagttc cggttcgcca gtgaaaggaa gagcgattga
      961 gttgaacttc aaacggcgca gcgcgccgag ttcaaaagcc agccagccat ttgggaattg
     1021 cagcataggt attaagaaaa aagttcgcgc aaagacgcag agtcgcaaag gaaagcggca
     1081 ccccgagtta acttggcgcc tttgcgcgaa tctctatttc gaaaacgacg aaaagagcct
     1141 ccagcggcgc ccgggtttcg tttcagactc acttacgggt tcatcttcgc cggttccggc
     1201 gaacgacgcc atctccggta gcggttcgag cgacagaaac acattgacaa cggccggatc
     1261 gaattcaatt cccgcacgat cggcaagctc gcgtcgcgtt tctgcttcgc ttaacgcagc
     1321 gcgaaacggt cgcgcatcgg tcagtgcggc ataagagtcg gcgacgcgca ggatccgcgc
     1381 cgccagggga atctcttcgc gtcgcagtgc gtcgggatag ccggcaccgt tccaccattc
     1441 gtgatgccag cgaaccagaa gttgcgccgc gcggtcggcg cctacgcgag acgcttcacg
     1501 ttcgccgatg atcggatgtc gcgcaaggtc gattctctct tcagtcgtca acgggcgctc
     1561 cacctgaata taatcgcgct ccatcgcaac ttcaccgagg tcgtgcaaca gcgctgccgc
     1621 atgtaacgaa ccgcgatcgt gacgcgccag atgaaacagc atcgcaagct cgtcagcaat
     1681 gatggcgata cgttgcgcgt ggcgatgatc gtagcgctcg aagcgatcag tcgttttcgc
     1741 gatctcggaa aacgcttcat cagttgtctt atcgattaag tcagtttgtt ggaatgcaac
     1801 catcgcgtcc aatttcatca cactgatttc aaagcgcgca acaggtccct ggccttctgg
     1861 ttgttgggat ccgacgcaat tgcgcgttca gcttcgccgc gcgcgcgcaa catcaatccg
     1921 agatcgcgat acaactccgc cagcatgacg cgatattcgc cgttggtcgg gtcgagcttg
     1981 agggccgctt gtaactcggc ttcagcggcg cgtcgcgtgt gttcgtgctc cgccaggagt
     2041 cttccgtaaa aagcgcgata gcgggcttct ttgggaacag cgttcgcggc tgaggcaaaa
     2101 agtccgactg ccgcttttcg ttgacccaat tcaagtgctt cgagaccttc cttgaaatga
     2161 agttcagcgc gttctgcgac ggagatagcg ggctcggaag gattggcagg cgcggcaccc
     2221 tgtggcgtcg ttggtgttgt tgtgggtttg gctgtggcgt cggcaagttg ttgcgctttg
     2281 cggcgcgctg caagttttgc atcgtagttc gcgcggagat tatcgtctcg aagcgtgtcg
     2341 tatgcctgag cgatgcgtgc gaaggccgtt tcgagacgag ccagcaacga cggctcggct
     2401 ttgcgaaaac ggtcggggtg gtaacgccgc gcgagttcgt agtacttgtc tttcatctgc
     2461 gcgggcgagg attctttgct gacgccaagc acgtcgtaaa acgtttcggc gctgttgagt
     2521 tgttccagaa aactcttcgc ctcgtcctgt tcgtccgccg gtttttccgg tgttggcggt
     2581 gcaggttggg gtggattttc ttgcgtggct gggagtgttt gggccgccgc gggctggcgg
     2641 cgaaacacat cgctccagtg ttctcgctcg agcaaacctg caagaacgag tgagtaaaca
     2701 attacgtagg tctcgttttc gcttatgccg ctgactgcga atagttcatt gagaggcgtc
     2761 ggccgatcga gtcgtgaaag cagaaagact tcggttggta gcaggttggt tgactcaggc
     2821 ggggatgacg ctggtgagat caactccgcc gaattgcgaa aacgcgaggc ggcaaacttc
     2881 ggtggcagtc gccggccggt atcgaggagc agagcgctgg tatcgatgtc gatgtttagt
     2941 ttctcgtcga gtcgggagcg tggatcgaat tcccaggcgc cttcggtcca cgaaagcgcg
     3001 agacgcagcg tgtctgtgac ttgttttgtt tggatttgct gggcggctgc gggagagagc
     3061 agatgatcgg ctgttagcgt cctggcgagt tcgatgtccg ggcgccgttg gtcgtagcga
     3121 gccagtgcgg cttcggcgat gccggctttc agtaggtatt ctcgcagtcg gagcgtgcgc
     3181 acgttggagg ttgcatacac gagttcgcct ttatcgaagt agatgactat cttgacgcgg
     3241 tcgtgttgga gttgcagctt tccggcaaga gactttgccg agatctcgcg aatcagttcc
     3301 gccagtggct gctcgctgag ttggccgttc atcctgatta catcgtaaac gaaaaccgtt
     3361 gaaacggttt cgagattttt agctgtgagt gtcagctgtt cgcgcgcgcg ccgacgtgtc
     3421 tgtcgcccct ccggggctcc cccgtttttt atcggttgat ccaggggcgg cgtgctccgc
     3481 tgcgctacgc ac
//
LOCUS       DASTEY010000245         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_812886, whole genome shotgun sequence.
ACCESSION   DASTEY010000245 DASTEY010000000
VERSION     DASTEY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812886"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..676
                     /locus_tag="VFO72_04380"
     CDS             191..676
                     /locus_tag="VFO72_04380"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET9478553.1"
                     /translation="MQRLWSPWRSKYIASGVDSQGEGCVLCHIAANPDQDGTNFVIHR
                     GEHAFVVLNLYPYITGHLMVVPYVHTSEFDSVAKEITDEMMDLAKRSQTALREVYSPS
                     GFNLGMNLGAAAGAGIADHIHIHLLPRWTGDTNFMTTVGESRVIPEALEDTYARLREK
                     F"
     gene            complement(792..>2649)
                     /locus_tag="VFO72_04385"
     CDS             complement(792..>2649)
                     /locus_tag="VFO72_04385"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9478554.1"
                     /translation="DDNEADRLKQDIILVDPRSPAAQVIVPAPRTRMVSLSAARQNLE
                     MRILSLQGWTPDQKTALTARLKPLIRPNVTLDQPTTATVRETEANKVKPVVITLKRNQ
                     VVAREGDTITPFVLSQINVIKEAGHRSLRWPNLLGLLVVVVAVYWAAWKFAERRRSSA
                     LSISKEKAFALVGSAILVQTALMKVGFTFGDSLAAQMQAPFNDPLIWNFAIPFAAASL
                     LVAMLVDTQLAFLVAMITALFAGMIAPGGMQKTLFAMVSCSAAIYGIGRYRERQSVTL
                     AGLLVGGVNAVMAVALLAYSGQSLTFRTFLMAVGCGIAGGLLTAVFAAGGLPINESLF
                     GILTDVKLLELSNADLPVLSQLALRAPGTNQHSHAVGQLAEDACRAVGANPLLVRIGA
                     LYHDIGKLAAPDYFVENQQGDNPHDRLRPYQSAKIITSHVTYGARLAKEIGLPKKIAD
                     FIPQHHGTRTLHYFLRKAQAQAKPNETIDEADFRYPGPKPQFKEAAIMMLADSCEAAA
                     RSLARPDPENIRAIVVKIVDAIISDGQLDECDVTLQELTIVREAMISALTAIYHARID
                     YPGFNPPLAGPLPAADIDSEERGVSYSKPSEVPISRGGEVEDEALPRKVGKR"
BASE COUNT          561 a          749 c          753 g          586 t
ORIGIN      
        1 caagacgggc gatcccgtta gcattcctgc gaagcgtgtg ccctatttca aaccgggcaa
       61 ggaattgaaa gaactcatca acgacgagac cgccgcatcc caggcttcac aaacgaattc
      121 ctcacgcggc ctgacagacg acaaccgcca accccctgtt ctttagtttg gttttacgag
      181 gtaattcgaa ttgcagagat tgtggagtcc ctggcgttcc aagtacatcg cgtcaggtgt
      241 cgattcacag ggtgaagggt gtgtgctgtg ccatatcgcc gccaatccgg atcaggatgg
      301 gaccaatttc gttattcatc gcggtgagca tgcgtttgtc gtgctcaatc tgtatcccta
      361 catcaccggg catctgatgg tcgtacctta cgtgcacacg agcgagtttg actccgtcgc
      421 aaaggagatc accgatgaaa tgatggatct cgccaagcgt tcgcagacag cattgcgcga
      481 ggtctactca ccgtccgggt ttaatctggg aatgaatctt ggtgcagcag cgggcgcggg
      541 catcgccgat catatccaca ttcatctact gccacgctgg accggcgaca cgaattttat
      601 gaccacggtt ggcgagtcgc gcgtgattcc ggaagcgttg gaagacacct atgcaagact
      661 tcgcgagaag ttctaagcca caaaagcatt gccgattgcc acttgccgat tgccaatttc
      721 tttgagtcgt gggcattcga tttcaattcg gcaatcggca atcggcaatc ggcaattggc
      781 aatgcttgtg gctagcgttt gccgaccttg cgcgggaggg cttcgtcctc gacttcacct
      841 ccgcgactaa tcggaacctc ggaaggtttg ctgtagctga cgccgcgttc ttcgctgtcg
      901 atgtcggcgg ctggtaatgg gcccgcgagc ggtggattga atcccggata gtcgatgcgt
      961 gcgtgataga tggcggtgag cgcgctgatc atcgcttccc ggacgatggt caactcctgc
     1021 aacgtcacgt cgcactcatc cagctgaccg tcgctgatga ttgcatcgac gatcttcaca
     1081 acgatcgccc ggatgttttc cggatcggga cgcgccagag atctcgctgc ggcttcgcac
     1141 gagtccgcca gcatcatgat cgcggcttcc ttgaattgcg gcttcggtcc gggataacgg
     1201 aaatcggctt cgtcgatcgt ttcgttcggt tttgcctgcg cctgggcttt gcgcagaaag
     1261 taatgaagtg tacgagtgcc gtgatgttgc ggaataaagt cggcgatctt ctttggcaag
     1321 ccgatctctt tcgccagccg cgcgccgtaa gtcacgtgac tggtgatgat cttggcgctc
     1381 tgatacggcc gcagccggtc gtgcgggttg tcaccttgtt ggttttcaac gaaataatcg
     1441 ggagccgcaa gtttgccgat gtcgtgatac aaggctccga ttcgcaccag taatggattt
     1501 gcgccgacgg cgcgacaagc gtcctcggca agttggccca cagcgtgtga gtgttgattt
     1561 gttcccggag cgcgcaacgc gagctgacta agcaccggca gatcggcgtt cgaaagttca
     1621 agcaacttga cgtcggtgag tatgccgaag agcgactcat tgatcggcag gccgcctgcg
     1681 gcaaaaactg cggttaacag gccgcccgcg attccacagc cgacggccat caggaaagtc
     1741 ctgaaggtga gtgattgacc cgaatacgcg agcagcgcta ctgccatcac cgcgttgaca
     1801 ccgccaacaa gtagtcctgc cagcgtaacc gattgccgct cgcgataacg gccgatgccg
     1861 taaatcgctg cggaacacga aaccatcgcg aacaacgttt tctgcatacc gccgggcgcg
     1921 atcatgcccg cgaacagggc tgtgatcatt gccaccagga acgcgagctg cgtatcgacc
     1981 agcatcgcca ccagcaaaga ggcagcggcg aagggaatcg caaaattcca gatcagcgga
     2041 tcgttgaaag gcgcctgcat ttgagcggcc agactgtcgc cgaaggtaaa cccaaccttc
     2101 atcaacgcgg tctgcaccag gatcgccgaa cccaccaacg cgaatgcttt ctctttgctg
     2161 atactgagcg cgctgcttcg ccgtcgttcg gcaaacttcc aggccgccca gtaaacagcg
     2221 acgacgacaa ccagcaaccc aagcagattg ggccagcgca gactccggtg accagcctct
     2281 ttgatcacgt tgatctgtga gagaacgaac ggcgtaatcg tatcgccttc acgcgctaca
     2341 acctgatttc gcttcaacgt gattacgacc ggcttcactt tattcgcttc cgtctcgcga
     2401 acggtcgcag tcgttggttg gtccagcgtg acgtttggcc ggatgagtgg ttttaacctg
     2461 gcggttaatg ccgtcttctg gtcaggcgtc caaccttgaa gactgaggat cctcatttca
     2521 aggttttgac gcgcggcgga aagcgacacc atgcgcgttc gcggcgccgg cacaatgacc
     2581 tgtgctgccg ggctgcgcgg atcgacgagg atgatgtcct gtttcaaacg atcggcctcg
     2641 ttgtcgtcg
//
LOCUS       DASTEY010000246         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_87756, whole genome shotgun sequence.
ACCESSION   DASTEY010000246 DASTEY010000000
VERSION     DASTEY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87756"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VFO72_04390"
     CDS             <1..1132
                     /locus_tag="VFO72_04390"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478555.1"
                     /translation="IDEETRAYMIYAFTQSGEGDTHFLDELYGKRNNLGPYGRALLAL
                     ALQERKDARAQEIAKLIEGAAKQNEFEAHWQTARVNDYGRDVYLDAEATALSLKALSQ
                     INPGSHLLPRAARWLVQNRRNGYYWLSTKETAFAIYGLTDYLKVSKELSPDYTFEVYL
                     NGIKVAGQHVGASEAVNAQTVTVWKKGGEVGPSNQIRIVKNGKGALYLSSALEYFTAD
                     ESVAAQSAGGLKITREYLRLRVSEDENGKAGWKVEALSGELRSGDMIVVKLRLTGPRA
                     QYVMIEDPIPAGAEQVARMSGLYLNYALGGWGDWYSNREFRDNRTAIFMNYFDGDATL
                     QYAMRVEVPGDFKIAPARAELMYQPTVQANTANDRLRILDKK"
     gene            1145..1999
                     /locus_tag="VFO72_04395"
     CDS             1145..1999
                     /locus_tag="VFO72_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478556.1"
                     /translation="MTTILKLWFSTIAKTFANPGALLVFAIIYAFLLVASYIFISIRE
                     ATILQVLVTYALMILIPIGFFTLQASIINRALDQKFRWRVILIDALKFLAVVIPLLLL
                     AWLLFYLLNKLAARYPAPVVEAPPPVNQGTPPPSATPALHWPSVIFTTLRFVLLGVAF
                     PLAAIHLWIAVAGGEFRGLFRDGAKSFFKRIGSALARAFSSDSVLIYGLGLIIFFVLP
                     YVILVPTFTVSGNKREFAFFGLRLLLAFLFSLVGWVATLTALTRTVPALPAEIPASNV
                     ALPTESAA"
BASE COUNT          452 a          594 c          681 g          540 t
ORIGIN      
        1 gatcgatgag gaaacgcgcg cttacatgat ttacgcgttc acccaaagcg gtgaaggcga
       61 tacccacttt cttgacgagc tctacggcaa gcgcaacaac cttgggccat atggtcgcgc
      121 gctactggcg ctggctttgc aggagcgcaa agacgcgcgc gcgcaggaaa tcgcgaagct
      181 gatcgaaggc gcagcgaagc agaacgagtt cgaagcgcac tggcagacag cgcgggtgaa
      241 cgactacggc cgcgatgttt atcttgatgc cgaagcgacg gcgctgtcgc tgaaggcgct
      301 gtcgcagatc aatcctggtt cacatctgct gcccagagcc gcgcgttggc tggtacagaa
      361 ccggcgcaac ggttactact ggctgtcgac taaggagact gcgttcgcga tctacgggct
      421 cactgattac ctgaaggtca gcaaggaact gtcgcccgac tacacgttcg aagtttactt
      481 gaacgggatc aaggttgcgg gacaacacgt cggcgccagc gaagccgtca acgcacagac
      541 tgtgacggtt tggaagaaag gcggcgaagt tggtccttca aaccaaatac ggatcgtgaa
      601 aaacggcaag ggtgctttgt atctatcgag tgcgcttgaa tattttacag cagacgagag
      661 cgtggcggcg caatctgcgg gcggattgaa gatcacccgt gagtatctgc ggctgcgcgt
      721 ctctgaggac gagaacggca aggcgggctg gaaggttgaa gcgctaagcg gcgagctgcg
      781 ttcaggcgac atgattgtcg tcaagctgcg actgactggc ccgcgcgcgc agtatgtgat
      841 gatcgaagat ccgattccgg ccggcgcaga gcaagtcgcg cgtatgagcg ggctttatct
      901 caattacgcg cttggcggat ggggcgattg gtatagcaat cgtgagttcc gcgacaaccg
      961 cacggccatc ttcatgaatt attttgacgg tgacgcgacg ttgcaatacg cgatgcgcgt
     1021 cgaggtgccc ggcgacttca agatcgcacc cgcccgcgcg gagctgatgt atcagccgac
     1081 ggtgcaggct aatacagcaa atgaccggtt gaggattctt gataagaagt gaaggcggaa
     1141 agttatgacc actattctaa aactttggtt cagcacaatc gcgaaaacgt ttgcaaatcc
     1201 tggtgcgcta ttggtgtttg cgatcatcta cgcgtttctg ctggtcgcgt cttacatctt
     1261 tatctcgata cgcgaagcga cgatcttgca ggtgcttgtt acctacgcgc tgatgattct
     1321 gattccgatc ggtttcttca ccttgcaagc gtcgatcatc aatcgtgcac tggatcagaa
     1381 attccgctgg cgtgtaatcc taatcgatgc cctgaagttc ctggcggttg ttattccgct
     1441 gctgctgctt gcgtggctac tcttctatct gctgaataag ctcgcggctc gttatccggc
     1501 tccggtggtg gaggcgcctc ctccggtcaa tcaagggact ccgcctccgt cagcaacgcc
     1561 ggcgcttcac tggcccagtg taatttttac cacgctgcgt tttgtgctgt tgggtgtagc
     1621 gtttccgctt gcggcaattc atctatggat tgctgtagcc ggtggtgaat tccgcggtct
     1681 tttcagagac ggggcgaagt cttttttcaa acgaattgga tcggcgctcg cgcgcgcctt
     1741 ttcgtcagat tcggttttga tttacgggct cggcctgatc attttctttg tgttgccgta
     1801 cgtcattctg gtaccgacgt tcactgttag cggcaacaag agggagttcg catttttcgg
     1861 tctgcgattg ctgctggcat tcctgttcag cctggtcggt tgggtagcga cgttgacggc
     1921 gctcacgaga actgtacccg ctttgcctgc cgagattccg gcgtcgaatg tcgcgcttcc
     1981 tacggagtct gccgcatgat ggtcagtttc gtggttggac gcggcatacc aactgtaggg
     2041 gcggcactcc gtggccgccc gtcgttcgaa atcgcattgt tcgtcaaacg ggcggccacg
     2101 gagtgccgcc cctacagttg gtactgacac gcatgagatt ttttgggcca ggaattcggc
     2161 aggttaaccg cgtgacgcgc gagcatggga tgctcctctc gtttgcgggc gcggcgattt
     2221 tgttcctcat actcccggcg cacaccaact ggggcgacag ttccaag
//
LOCUS       DASTEY010000247         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_71237, whole genome shotgun sequence.
ACCESSION   DASTEY010000247 DASTEY010000000
VERSION     DASTEY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71237"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFO72_04400"
     CDS             <1..339
                     /locus_tag="VFO72_04400"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HET9478557.1"
                     /translation="PSTAKNTIPAHYTQAPNRASLGPTLEPEQFTGLVRDAYRAVREI
                     PQTIAQMPCYCHCDRGMGHKSLYSCFEDDHASHCAVCVNEALLALKLEKQDKLTPAQI
                     RERIVQQYTQ"
     gene            complement(590..1912)
                     /locus_tag="VFO72_04405"
     CDS             complement(590..1912)
                     /locus_tag="VFO72_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9478558.1"
                     /translation="MFVITFEILFIVVLLIANGVFAMSELAVVASRKTRLQRMADLGD
                     TRASAALELAQQPDRFLSTIQIGITLIGIFAGAVGGATIAEQLGARISTIPSLAPYGE
                     AIGLIVVVLSITYLSLVIGELVPKRLALSHPERVALVVAGPMRGLARLASPVVSILSV
                     STSAVLTLLRMRAPTEPPVTEDEIKVLIQQGTQAGVFEEVEQEMIESVFRLADRSVEA
                     LMTPRMDIVWLDVNDPPDKLHRKVAESTYSRFPVCRGHLDNLLGIVKAKEMLSRCVSG
                     APLDLQAALKQPLFIPESTRAIRALEMFKAARTHLALVIDEYGAVEGLVTTNDILEAI
                     VGDIAPAQSDAGRDAVQREDGSWLLEGALPFDEFREIFSVERLPGEERGLFHTLAGFI
                     LFHLGRVPSEADHFEWHGLRFEIVDVDGKRIDKVLVMAAKPSVATRRE"
     gene            complement(2458..2820)
                     /locus_tag="VFO72_04410"
     CDS             complement(2458..2820)
                     /locus_tag="VFO72_04410"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HET9478559.1"
                     /translation="MARKGFLTPGILVACLALTFAPACSREPGEKPSTSAPASGPAAA
                     VQSKQYEAVGVVKAVNPEGPAIEIDHEEIAGLMPAMQMEFAVTDANVLNGLAVNDRID
                     FTVENAIDGMKVIAIKKK"
     gene            complement(2899..5682)
                     /locus_tag="VFO72_04415"
     CDS             complement(2899..5682)
                     /locus_tag="VFO72_04415"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9478560.1"
                     /translation="MKIRIFVISICLLVSTPALSVAQQNVTSGTLSGRIEDSRGAVVS
                     GANVTATNLETNQRLATTSDEQGRYRFPYLRTGAYDLTVEAAGFSAVTKHMNVLVGQA
                     LEFPITLDVAGVSAQVNVGTELPLVETVRTQITETIHPREITDLPLNGRNYLDLALLV
                     PGVSPTNTGSNQRFAETSAVPGQGISIAGQRNLYNSFIVDGISANDDAADLTGTYYSE
                     EVIDQFQVVTSGGIAEFGRASGGVVNILTRAGTNDFRGNVYGFFRNQRFDARNPLAPT
                     KDLLTQAQYGGTFGGPLRRDRSFFFTNFEQTRRNYSAVITIAPAAVNTINNRLRAVNY
                     PGALIETGVVPASFDTTNFFIRIDHRLNARNQLSGRYSLYHIEAANSRTVGGLNAVSR
                     GSGLNDTDQTMQVTNVTTLSNRTVNEARFQLTNSRLAAPVNDTIGPAVGISGVANFGT
                     ATFSPLARDINLFEVVDNVSTQFGAHSLKAGGGFLYNRLNILFPGAFQGVYNFTSLNN
                     FLNGNYSTYQQAFGVPNQFQSNPNFGVFIQNEWRARRDLTINAGVRYDVQYLPDPIET
                     DKNNIAPRLGIVYAPGDRKTVIRASYGLYYDRIPLRATSNALQRDGSKYLIAQLAPTQ
                     SGAPVFPNLLSLQPTTLPAKPNITRIDPNIEVSYSQQGNFQIERELPRDAVVSVGYLH
                     VRARHLILSRNVNVPRFPASAGVPNLGRPDPDWGNVSRFESSGNSCYDGMVVSFNQRA
                     SQWATVRLSYTLSKTIDDAGNFFFSSVQDNFNIRDDRGLSDNDQRHRLVLSGTFEAPQ
                     ESKSRGATLLSGFQFGYIFTYASSLPFNVLLGTDRNFDTNFNDRPIGVARNTGRGFDF
                     ASLDLRVSRRFRLTERVDLQLLAESFNVLNRANFGVPNNTFGPGVNALPTFGRPTQAF
                     DPRQFQFGMKVSF"
BASE COUNT         1321 a         1665 c         1716 g         1271 t
ORIGIN      
        1 ccgtcaaccg ccaagaacac aattcccgcg cattacacac aagccccgaa ccgcgcgagt
       61 cttggtccaa cattggaacc ggaacagttc accggactcg tgcgcgatgc gtaccgcgcg
      121 gttcgtgaaa ttccgcaaac gatcgcgcaa atgccctgtt actgccactg cgaccgcggc
      181 atgggacaca agagcctcta cagttgcttc gaggacgatc acgcgtcgca ttgcgcggtc
      241 tgtgtgaacg aagccttgct cgcacttaaa ctcgagaagc aagacaagct aacgccggcg
      301 caaatccgtg agcgcatcgt gcagcaatat actcagtgaa cggaggagta gggtgatctg
      361 aagcaattct ttgccgcgat tgccagctaa agccttgccg aaagttcaaa gttgattcga
      421 atcaagtacg gttctgttct attggccgaa agactttcaa gacggattga actcagatgt
      481 aatgggagcc ctgcgtaacc cgcgaatgaa cgtcggttcc ttgtgaactc cagcggagca
      541 tctccgcacc aatggtctgg tccataactc ttcccttgaa ctctttcaat tactcacggc
      601 gcgttgcaac ggacggtttt gctgccatga ccagcacctt gtctatgcgc ttgccgtcca
      661 cgtccacgat ctcgaagcgc agcccatgcc actcgaagtg atcggcctcg gatggtacgc
      721 gccccagatg gaaaaggatg aagcccgcga gcgtgtgaaa taggccacgc tcctcgcccg
      781 gcagcctttc gacggagaag atctccctga actcatcaaa gggcaaagcg ccttcgagca
      841 gccacgagcc gtcctcacgc tgaacggcgt ccctgcccgc gtccgactgc gcgggcgcga
      901 tgtcccccac aattgcttcc agtatgtcgt ttgtagtcac aagtccctcg acggccccgt
      961 actcgtctat cacgagcgcg agatgcgtac gcgcggcctt gaacatctcg agcgcccgaa
     1021 tggctcgggt cgattccggg atgaagagtg gctgcttcaa agctgcctga agatcgagag
     1081 gcgcgccgga cacgcagcgc gagagcatct ccttggcctt aactatcccg agtaagttgt
     1141 ccaggtggcc gcggcagacc ggaaacctcg agtaggtact ctcggctacc ttccggtgaa
     1201 gtttgtcggg tgggtcatta acatcgagcc aaacgatgtc catgcgtggc gtcatcagcg
     1261 cttcgacgct gcgatccgca agcctgaaga cactctcgat catctcctgt tcgacctcct
     1321 caaacactcc ggcttgcgtg ccctgttgga tcaacacttt gatctcatcc tcggtcaccg
     1381 gaggctccgt cggcgctctc atacgcaaca atgtgagaac cgccgaggtc gagacactca
     1441 gaatgctcac cacgggtgaa gccagccgcg ccagtccgcg catcgggccg gcaaccacta
     1501 aggcgacgcg ttccgggtgg ctcagggcga gacgcttcgg gaccagttcg ccaatgacca
     1561 gcgaaagata tgtgatgctc agcaccacga ctattaaccc tatggcctca ccgtagggtg
     1621 cgagcgacgg gatggtactg attcttgcgc ccagttgctc ggcaatggtc gcgccgccga
     1681 ccgccccggc gaagatgccg atcagagtga taccgatctg gatcgtcgag aggaagcgat
     1741 ccggctgctg cgccagctca agagccgcac tcgctcgcgt gtctccaagg tcggccatcc
     1801 tttgtaggcg ggtcttgcgc gaagcgacga ccgccagttc cgacatggcg aagacgccgt
     1861 tagcgatgag cagcacaacg ataaagagaa tctcgaaagt gatgacgaac attttgcttc
     1921 atcctctaag gcgtcccttg gcgtgagccg cctccgccaa cgcctcagtc tcgcggggta
     1981 accccgcccg gttcagatct ttgatcgcct ccttcgagca atccgagccg tcgtaggcga
     2041 ttaatatttt catcttctcg ttcatcgctc cacctctttt tcgttcagcc tgtgtgaata
     2101 gatggccgcg aacccgcagt gtaggattag caggctcaag cgacacttac tttcagcgag
     2161 cgctagacga agagcggcgg gctgcggccg gaaaggattg agacggaatg atagagggac
     2221 tgtacctggg cggcgacggc ccaccggtag atatttggtg atagctcgtg cgcacacggc
     2281 ggagctggag tcgcccaagc ctgaaattga cgcttgatac gtttctgcgc aacagatgtc
     2341 gtccctttgc gagtagtgac gccgtgaggg gtcacctcat cagttaggaa cagtaaacac
     2401 tagtaacaat ataatgaaga acacccgttt tcaaatgcgg actagacgta aggctcgcta
     2461 cttctttttg atcgcaatca ccttcatccc gtcgattgcg ttctcaaccg tgaaatcaat
     2521 ccggtcattg actgccaacc cattcaacac gttggcgtca gtgaccgcaa attccatttg
     2581 catcgcaggc atcagtcccg cgatctcttc atgatcgatc tcgatcgccg gcccttccgg
     2641 gtttacggct ttcactacgc cgaccgcttc atactgcttc gattgaacgg cagccgccgg
     2701 accggaagcc ggcgccgaag ttgaaggttt ctcaccaggc tcgcggctac acgccggcgc
     2761 gaacgtcaac gccaaacaag cgaccagtat tccgggcgtt agaaagcctt tgcgagccat
     2821 gcgcttctct tctcacccgt gtttgtaaca tacgcaacgg ccaatttgtt gcgcgcccaa
     2881 aatccgtgtc atctgtggtt aaaaactcac tttcattccg aactgaaact gccgcggatc
     2941 gaacgcttgc gtcggccgtc caaaggtcgg aagcgcatta acgcccggcc cgaacgtatt
     3001 gttcggcacg ccaaagtttg cgcggttcaa aacgttgaaa ctctccgcga gcagttgtag
     3061 atcaacgcgt tcagtcaaac ggaacctgcg actcacgcgc agatccagcg aagcgaaatc
     3121 gaagccacgg ccagtgtttc tcgccactcc gatcggacgg tcgttgaagt tggtgtcgaa
     3181 gttccgatcg gttcccaaca acacgttgaa cggcaaactc gacgcataag taaagatgta
     3241 gccaaactga aaaccactga gcagcgtggc gccacgcgac ttactctctt gcggtgcttc
     3301 aaacgtgcca ctcagcacca ggcggtgacg ctgatcgttg tccgaaaggc cgcgatcgtc
     3361 gcggatgttg aagttgtcct gcacactcga gaagaagaaa ttgccggcat cgtcgatcgt
     3421 cttcgagagc gtgtacgaaa gacgcaccgt agcccactgc gaagcccgct ggttgaatga
     3481 aaccaccatg ccgtcgtaac aggaattgcc ggagctttcg aaccgggaga cgttgcccca
     3541 gtcgggatca ggccggccga gatttggaac acctgccgat gcgggaaacc tcggcacgtt
     3601 cacgttgcgc gaaagaatca ggtgcctggc gcgcacgtgc agataaccga ctgacaccac
     3661 cgcgtcccgc ggcagttcgc gttcgatctg gaagttgccc tgttggctgt agctcacttc
     3721 gatattcgga tcgatgcgcg tgatgtttgg tttcgccggc agcgtcgtgg gttgaagcga
     3781 caacaggttg ggaaacaccg gagcgcctga ttgcgtgggt gcgagttgcg ctatgagata
     3841 tttcgagccg tcgcgttgca aagcattcga cgtagcgcga agcggaatac gatcgtagta
     3901 cagtccataa ctcgccctga ttaccgtctt gcgatcgccg ggcgcgtaaa caatgccaag
     3961 ccgcggcgca atattgttct tgtcagtctc aatcgggtcc ggcaagtact gaacgtcgta
     4021 tctcacgccc gcgttgatgg tgagatctcg ccgcgcacgc cactcgttct gaataaacac
     4081 gccgaagttg gggttagact gaaactggtt gggaacgcca aacgcctgct gataagtgct
     4141 gtaattcccg ttaagaaaat tgttgaggga agtgaagttg tagacgccct gaaaagcgcc
     4201 ggggaaaagt atgttcaggc ggttgtacag aaagccgccg ccggctttca gcgaatgcgc
     4261 gccgaactga gtcgaaacat tgtcgacaac ttcaaacaga tttatgtcgc gagcgagcgg
     4321 cgagaaggtc gcggtgccga agttagcgac gccggagata cccacggctg gtccaatagt
     4381 gtcgtttacg ggcgcggcga ggcgactgtt ggtaagttga aaccgcgcct cgttgaccgt
     4441 gcgattgctg agggtagtga cattagtgac ctgcattgtc tgatcggtgt cgtttaatcc
     4501 tgaaccacgg ctgacggcgt ttaagccgcc gacggtgcgc gagttcgcgg cttcaatgtg
     4561 atacaggctg taacgtccgc tgagttggtt gcgcgcgttg aggcggtgat cgatgcggat
     4621 gaagaaattt gtcgtgtcga aactcgccgg cacgacgccg gtctcaatca acgcaccggg
     4681 ataatttacc gcgcgcaggc ggttgttgat tgtgtttacg gctgcgggag cgatggtgat
     4741 gacggcggaa tagtttctgc gagtctgttc gaaattggtg aagaagaaag atcgatcgcg
     4801 gcgcaacgga ccaccgaaag tgccaccata ctgagcttga gtcagcagat ctttggtggg
     4861 cgccaacgga ttgcgcgcgt caaaacgctg gttgcggaaa aagccataga cattgccgcg
     4921 gaaatcattg gtgcccgcgc gcgtgaggat attcacgaca ccgccggaag cacgtccaaa
     4981 ctcagcgatg ccaccggaag taacgacctg aaactgatcg atcacctctt cgctgtagta
     5041 agtgccggtc agatccgcag catcatcgtt tgccgagatg ccatcgacga tgaaactgtt
     5101 gtagaggttc cgttgtccgg cgattgatat gccctggcca ggcacggccg aggtctcggc
     5161 gaagcgttgg ttactgccgg tgtttgtcgg tgaaactccc ggaaccagca atgccaggtc
     5221 gagatagttg cgtccattca gcggcaagtc tgtgatctcg cgcgggtgaa tagtttcggt
     5281 gatctgagtg cgcacggttt cgacgagcgg cagttcggtc ccaacattca cttgagctga
     5341 cactcctgcg acatcgaggg tgatcggaaa ctcgagcgcc tggcccacca gcacgttcat
     5401 gtgtttggta acggccgaaa aacccgcggc ttcgacagtc aagtcatagg cgccggtgcg
     5461 caggtaaggg aagcgataac gtccctgctc gtcggacgtg gttgcgagtc gttgatttgt
     5521 ttccaggttg gtcgcggtta cgtttgcgcc gctgaccact gccccgcgtg agtcttcaat
     5581 gcgcccgctt agcgtgccgg atgtgacgtt ttgttgagcc acgctgagag caggtgttga
     5641 gaccaggagg cagatggaga ttacgaaaat acgaatcttc atcgaacttc ctccaggatc
     5701 gtgattgatg tagttgttag acgatctgct ggagggcgat aggtggacca cgcgggctta
     5761 cttgcgccca ggttctggca gatgaaaaga gagaagtgcg ggctttgtaa tgcgaagaag
     5821 ttttgtaagg tgaatcgtgt ctcgcgacct gggttgctcc cggttcgcgt ttctgttgcc
     5881 gcgtcgaagt caaacacgcg cagcagtcag agtgacaaga gttacggtcc gcggatctga
     5941 aagccggagc tgatgaaacg gatgcatctt cct
//
LOCUS       DASTEY010000248         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1340247, whole genome shotgun sequence.
ACCESSION   DASTEY010000248 DASTEY010000000
VERSION     DASTEY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340247"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..662)
                     /gene="yihA"
                     /locus_tag="VFO72_04420"
     CDS             complement(72..662)
                     /gene="yihA"
                     /locus_tag="VFO72_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HET9478561.1"
                     /translation="MKIRGPFKAEFLKSAFKEADWPRDSKPEIAFLGRSNVGKSSLIN
                     SLLGVRGLARTSSTPGRTQSLNFFTINDQFRFVDFPGFGYARVPGKIKSSWGEMATTF
                     LAKRSQLVLSIHLVDSRHEPTKQDLQLHEWLKEYDKPRLIVATKSDKLSNNELRRNLE
                     HIARVLNDDSVMAYSAKSGRGRNELWRAIESAIERS"
     gene            complement(717..1172)
                     /locus_tag="VFO72_04425"
     CDS             complement(717..1172)
                     /locus_tag="VFO72_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478562.1"
                     /translation="MSKYKDADLILKLYDLRREKTMREARNWFFTFNPQGPQDFIDVL
                     TSEKSGLYRMVISYWDMACSLVNNGAIDADMFNDANGEHIFVYAKMEPFIPVLREQLG
                     APQFLAHLEKTVKAIPDYEQRLATIRERMAKLGEIWKQKNAAQAAAATN"
     gene            complement(1218..>3611)
                     /gene="lon"
                     /locus_tag="VFO72_04430"
     CDS             complement(1218..>3611)
                     /gene="lon"
                     /locus_tag="VFO72_04430"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9478563.1"
                     /translation="ADVAWYPMVPIRDVVIFPFTKVAFKIGRPGSVRALEQALASDRT
                     IFLATQHDATVDEPSPQQIYGVGTLGRILQSQKQENGQIKVVVEGRERATTVRVENSD
                     GAYMALVRRAPIVNEEGTRLDALIQKVGQLVEQHLRLAPDTQTDALQTALRNQEPSHL
                     ADALASQLRISVEDKQGLLEIFSTQARLQRLIELLETEIEKRQLDRTIQTRVKRQMEK
                     AQKEYYLNEQIKAIHKELGRKDEKAELEELKKKIEEAGMTDEAKEKAMQELHRLEAMP
                     PMSAEGTVSRTYIDWLISVPWKQKSKEIKDLTKAEEVLNDDHFGLEKIKERILEYLAV
                     RQLVKNPKGSILCFVGPPGVGKTSLGKSIARATGRKFVRLSLGGVRDEAEIRGHRRTY
                     IGALPGQIIQMMKKAATVNPVILLDEVDKLGADFRGDPSSALLEVLDPEQNHTFQDHY
                     LDVEYDLSKVFFIATANVLHTIPPALQDRLEILRLSGYTEREKLEIAKRHLVPKQAEG
                     NGLKPEQIEFTDEGILEVIRHYTREAGVRNLEREIGSCCRKIARKFVSENLGEAAKDS
                     IDAARVREMLGPIKFRQQGVAAHSEIGLGTGLAWTEIGGEVLQIEATLVKGRGGVTLT
                     GKLGDVMQESAQAALTCIRARAERLGMSVDFIRKRDLHIHIPEGAIPKDGPSAGITMA
                     TAMASALARVPVKKNVAMTGEITLRGRVLPIGGVKEKLLAAHRFGIDTIILPKDNEKD
                     LSEIPEEVRDALSIYFVETIDEVLSLALEEACPTEAAAGEGPPLWTTEQPSQGIQTS"
BASE COUNT          705 a         1000 c         1008 g          898 t
ORIGIN      
        1 gtttcaggat acaagcttac tgttgaaaag cgcaaacgag aatttcttct ctgttgcata
       61 gagatgtcaa tttagctccg ctcgattgcg ctctctattg cacgccaaag ctcgttgcga
      121 ccgcggccgc ttttggccga gtaggccatt acgctgtcgt cattgagcac gcgcgcgatg
      181 tgctccaggt tccttctcaa ctcgttgttc gaaagcttat cggattttgt ggcaacaatt
      241 aaccgcggct tgtcatactc cttaagccac tcgtgaagct gcaaatcctg ttttgtgggt
      301 tcgtggcgcg aatctacgag gtgaattgat agcacaagct ggctgcgctt ggcaagaaaa
      361 gtggtcgcca tctcacccca cgacgatttt attttccccg gtacgcgagc gtacccaaag
      421 cccggaaagt ccacaaaacg aaactgatcg ttgatagtga agaagttgag agactgcgtc
      481 cggccggggg tggaggaagt tcgggcaagt ccacgaacac cgagcaggga gttgatcagg
      541 ctcgatttgc cgacattcga tcggccgaga aaggcgattt cgggtttgga gtctcgcggc
      601 cagtcagctt ctttgaaggc acttttcaga aactccgctt taaaagggcc acggattttc
      661 acggacaaac gcggattggc tctgtgttca ttagtaaaat ccgtggcctg ccttcattag
      721 ttggtggcgg ctgcggcctg ggcggcgttc ttctgtttcc agatctcgcc gagcttcgcc
      781 atgcgctctc gaatcgtcgc gaggcgctgc tcatagtccg gaatcgcttt caccgtcttc
      841 tcgaggtgcg ccaaaaactg cggcgctccc aattgctccc gcaagacggg gataaatggt
      901 tccatcttcg cgtaaacaaa gatgtgttcg ccgttggcgt cgttgaacat gtcggcatcg
      961 atcgcaccgt tgttgacgag cgagcaggcc atgtcccagt aagaaatcac catccgatag
     1021 aggccgcttt tttcgctggt caatacgtct ataaaatcct gcgggccctg ggggttgaaa
     1081 gtgaagaacc aattgcgtgc ctcgcgcatc gttttctcac gtcgaagatc gtagagcttc
     1141 aaaatcagat cggcatcttt gtatttgctc atacttttcc ctctctagtt tattaaaacg
     1201 gatgttggga ataagagtta gcttgtctga atgccctgcg acggctgctc ggttgtccac
     1261 aatggcggac cttctccggc agctgcctca gtcgggcagg cttcctcgag agcgagtgac
     1321 aacacctcat caatcgtttc cacaaaatag atactgagcg cgtcgcgcac ctcttccgga
     1381 atctcgctga gatccttttc gttatcttta ggcaggatga tggtgtcgat accaaaacga
     1441 tgtgcagcca ggagtttctc tttcacaccg ccgattggca acacgcgtcc gcgcagcgtg
     1501 atctcgccgg tcatcgcgac gttctttttc accggaacgc gtgcgagcgc ggaagccatt
     1561 gcggtagcca tggtgatgcc cgcagaggga ccatccttgg gaatggcgcc ttcgggaatg
     1621 tgaatgtgca aatcgcgttt gcgaatgaag tcgacgctca tgccgagacg ttcggcgcgc
     1681 gcgcgaatgc acgtgagcgc agcttgcgcc gattcctgca tcacgtcgcc gagtttgccg
     1741 gtgagcgtaa ccccaccgcg gcctttgacg agcgtggctt ctatctggag cacttcgccg
     1801 ccgatctccg tccaggcgag tccggtacca agaccgatct cgctatgagc cgcaacgcct
     1861 tgctggcgaa acttaattgg gccaagcatc tcgcgaacgc gcgccgcatc gattgaatct
     1921 ttcgccgctt cgccgaggtt ttccgagaca aacttgcgcg ctatcttgcg gcagcaggag
     1981 ccaatctctc tttcaagatt gcgcacgccc gcttcgcgag tgtagtgacg gatgacttcc
     2041 agtatgccct cgtcagtgaa ttcgatctgt tcgggtttga gcccgtttcc ttcggcctgt
     2101 ttcggcacga ggtgacgttt ggcgatctcg agcttctcgc gttcggtgta acccgaaagc
     2161 cgcagaattt cgagacgatc ctgcagcgcc ggcgggatcg tgtgcagcac gttcgcagtg
     2221 gcgatgaaga acactttcga gagatcgtat tccacgtcga gatagtggtc ctggaacgtg
     2281 tggttctgtt ccggatcaag cacttcgagt agcgccgaac tcggatcgcc gcgaaagtca
     2341 gcgcccaact tatcgacttc gtcgagcagg atcaccggat tcacggtggc ggccttcttc
     2401 atcatctgaa tgatctggcc gggcagcgcc ccgatgtacg tgcgacgatg gccgcgaatc
     2461 tcggcttcgt cacgaacgcc gccaagtgac agcctgacaa acttgcgtcc cgttgcgcgt
     2521 gcgatcgatt tgccgagact ggttttgccc acgcccggcg gtccaacaaa acagagaatc
     2581 gaacccttgg gattcttcac caactggcgt accgcgagat actcgagaat gcgttccttg
     2641 atcttttcga gcccaaagtg atcgtcgttg agcacctcct cggccttagt gagatctttg
     2701 atctctttcg acttctgttt ccacggcacg ctgatcagcc agtcgatgta agtgcgtgaa
     2761 acggttcctt cggccgacat cggcggcatg gcctcgagac ggtgcagttc ctgcatcgcc
     2821 ttctctttgg cttcatcggt catgcccgct tcttcgatct tcttcttcag ctcttcgagt
     2881 tcagccttct catctttgcg gccgagctct ttgtgaatgg ctttgatctg ctcgttgagg
     2941 tagtactcct tctgggcctt ctccatctgg cgcttcacgc gcgtttggat ggtgcgatcg
     3001 agttgacgtt tttcgatctc agtttcgagt agctcgatca atcgctggag gcgagcctgc
     3061 gtggagaaga tctcgagtag tccctgcttg tcttcaaccg agatgcgcag ttgcgaagcg
     3121 agcgcgtccg cgagatgcga cggttcctga ttgcgcaacg cggtttgcag cgcatcagtt
     3181 tgtgtgtcag gcgcgagccg caagtgctgc tctacgagtt gaccgacttt ctgaatgagt
     3241 gcgtcgagtc gcgtgccttc ttcgttgacg ataggagcgc ggcgcacgag cgccatataa
     3301 gcgccgtcgc tgttttccac gcgcacagtg gtggcgcgtt cgcgtccttc gacaacgact
     3361 ttgatctgtc cgttctcctg tttttgtgac tggagaatcc gtccgagtgt gccgacgccg
     3421 taaatttgct gcggcgacgg ctcgtcgact gtcgcgtcgt gctgagtagc aagaaagatt
     3481 gtccgatcac tcgcgagcgc ttgttcgagc gcgcgcacgg aaccgggacg accgatcttg
     3541 aaagcaactt tagtaaaggg gaatatgacg acgtcgcgaa tcggcaccat cgggtaccag
     3601 gctacgtctg c
//
LOCUS       DASTEY010000249         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_994313, whole genome shotgun sequence.
ACCESSION   DASTEY010000249 DASTEY010000000
VERSION     DASTEY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994313"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFO72_04435"
     CDS             <1..659
                     /locus_tag="VFO72_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9478564.1"
                     /translation="PVVVRVPIGGYLKGGAVYHSQSGVSTFTQIPGLRVVYPSTALDA
                     NGLLRTAIRCDDPVMFLEHKHLYRQAYNKSAYPGDDFMIPFGKAKRVREGKDLTIVTY
                     GALVQRSIVAAKQAEQEGISVEILDLRSLAPYDWNAITESVKRTNRVIVAHEDSLSFG
                     YGAEIAARIADELFEYLDAPVKRVCALDTFVAYAPQLEDAILPQPSDVAKAIAEIKAY
                     "
     gene            889..1824
                     /locus_tag="VFO72_04440"
     CDS             889..1824
                     /locus_tag="VFO72_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478565.1"
                     /translation="MSKHVSSVLAAVILTLTLSALAFAQTTTTTVTQTVQNPDGTYTI
                     IEYPAKKEVTLTLNPVTIQGAKGVATILRDDDGTRIKLNLTDVPADVSALTLYAVDDM
                     GKVTAIGPVTISNGVGTLTATAPLTRFMLIASPETALTMYDANTKVFFRSAVPQGFAV
                     IPVQTNPAGEKVAAVAAPTTTPAGTTYTAPLLNIPAFKSGDDTKIKVDFTGALTGARA
                     NVFITPHKDKPTEVKMRFHDLKEAPAGQKYVVWAVGADGTYQKLGEIVNTGGRNEAEF
                     KAETTLADFGLLLTMESALGAAPAGPAVATIHIVP"
BASE COUNT          462 a          579 c          583 g          435 t
ORIGIN      
        1 cgccggtcgt ggtgcgtgtg ccgattggtg gttatttgaa aggcggcgcg gtctaccact
       61 cgcagtcagg cgtgtcgacc ttcacacaga ttcccggttt gcgtgtcgtc tacccatcga
      121 cagcgctcga tgcgaatgga ctgctgcgca ctgcgatccg ctgtgacgac ccggtgatgt
      181 ttctcgagca caagcatctc taccgtcagg cttacaacaa gtcggcctac ccgggcgacg
      241 atttcatgat tccgtttggc aaggcgaagc gagttcgcga aggaaaggac ctcacgatcg
      301 tcacgtacgg agcactggtg cagcgttcaa tcgttgcggc gaagcaagca gaacaggagg
      361 ggatcagtgt cgagatcctc gatctgcgca gtctcgcgcc gtacgactgg aacgcgatca
      421 ccgagtccgt gaaacggaca aaccgcgtga tcgtcgcgca tgaagattcg ctttcgtttg
      481 ggtacggagc ggagatcgcc gcgcggattg ctgatgaact gttcgagtat ctcgacgctc
      541 ccgtcaaacg cgtttgcgct ctcgatacct ttgtggccta cgcaccgcaa ctcgaggacg
      601 cgattctgcc gcagccctca gacgtagcca aagccatcgc ggaaatcaag gcttactgac
      661 aatttagcca caagttcgtg agcagacctg cggggctata gccgtacagc cgtgtattgt
      721 tctgtttcgc gtagactgct cctgccagac attttgcgtt aaaaaaacca accgcccaaa
      781 cggcataaat attgccgact catcgctata tacgcctgcg cgcgtctccc cggcaccgtt
      841 cccccgacaa ttaatcgcga ttgattagac acaagggagg tacaactgat gagcaaacac
      901 gtttcgagtg ttttggccgc tgtgattctg actctcacac tgtcggcact cgctttcgca
      961 cagacgacaa ctacaaccgt tacgcagaca gtccaaaacc ccgacggaac gtacaccata
     1021 attgaatatc cagcgaaaaa agaagtaaca ctgacgctga acccagtcac catccagggc
     1081 gccaaagggg tggcgacaat tctgcgcgac gacgatggca ccagaattaa actcaacttg
     1141 acggacgtac cagcggatgt ttctgcgttg actttgtatg cggtcgatga catgggcaag
     1201 gtaacggcta tcggccccgt gacgatcagc aacggtgtag gcacgctgac cgcgacggcg
     1261 ccgttgacca ggtttatgtt gattgcttcg cctgagactg cgctgactat gtacgacgcg
     1321 aacacgaagg ttttcttccg cagtgcagtt ccgcaagggt tcgcggtgat tccggtgcag
     1381 accaacccgg ctggtgagaa ggttgcggct gttgcggctc cgaccacgac gccggctgga
     1441 acgacctata ccgctccact gctgaacatt ccggctttca agtcaggcga tgacacaaaa
     1501 atcaaggtgg acttcactgg cgctttaacc ggcgctcgtg cgaatgtgtt catcacgccc
     1561 cataaagaca aaccgacgga agtaaagatg cgttttcacg atttgaagga agcgcccgcg
     1621 ggccagaagt acgtcgtctg ggccgtcggt gctgatggca cgtatcagaa gctcggtgag
     1681 atcgtgaaca cgggcggacg caacgaagcc gaattcaagg ctgagactac gttggcagac
     1741 tttgggttgt tgttgactat ggaatcagcg ctcggcgcag cgccggcagg gccggccgta
     1801 gccacgattc acattgttcc atagctgaaa aaccatcgtt tgatgttggg ccgtgcctca
     1861 ggcgcggccc ttttcttctg tttttgcgcc tcagcgccga aggcgcgttg agaaggtagc
     1921 cagcggcaag tgcgagcgaa gcgagcacgc cgcccctgga tcattcaaac aaatcgagcg
     1981 agcccggaac gggcgacaga catgtcggcc gtgccgctct ttgtccgccc tccgggcctg
     2041 atatttattt ttcctgtga
//
LOCUS       DASTEY010000250         7676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_812953, whole genome shotgun sequence.
ACCESSION   DASTEY010000250 DASTEY010000000
VERSION     DASTEY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812953"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3160)
                     /locus_tag="VFO72_04445"
     CDS             complement(<1..3160)
                     /locus_tag="VFO72_04445"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478566.1"
                     /translation="MFKTFRASRHTVILRLVVALVLSTVAVQAQRPQPSPTPAPFDLL
                     KPLQYRNIGPYRGGRSAAVAGVASQPFVYYYGATGGGVWKSTDGGINWESVSDGSVFG
                     TGSVGAIAVSDSDPNVVYVGMGESPIRGNVSHGDGVYKSTDAGKTWKRIGLEDTRQIP
                     RIRVHPKNPDLVYVAALGHVWGPNDQRGVFRSKDGGKTWEKILSRGNKAGAIDLILDP
                     TNPSIIYAGFWEVYRKPWTLESGGPGGGIFKSTDGGDTWTELTRNPGLPRGLVGKVGI
                     TVSPANPERVWAIVEAEDGGVFRSDNGGRTWTKVNEERRLRQRAWYYTRIYADPKNAD
                     SVYVLNVGFFKSNDGGRTYTTISVPHGDNHDLWIAPDDPNRMIESNDGGANVSFNGGR
                     SWTEQDQPTAQFYRVTVDNDFPYNIYGAQQDNSTIRIASRTTENSITEKDWFDVGGGE
                     SGWIAPSPKDSNIVFAGSYGGLLTRYDHRTGQQRNISPYPNNPMGSGADVLKYRFQWN
                     FPIVFSPHDPNTLYSAANVLFKTNDEGSSWQIISPDLTRNDKSKQAASGGPITKDNTS
                     VEYYATIFAVMESPLQAGTIWTGSDDGVVHLTRDGGKNWSNVTPPASIMPEWIQINSL
                     EASPHDPATAYLAGTMYKWDDNRPYLYRTSDYGKTWKKITNGIPDTAFTRVIREDPNR
                     RGVLYAGTETGMYVSFDNGERWQSLQLNLPVVPITDLAVHKREKELVVATQGRSFWIL
                     DDLPLLHQLMDAGGFNSVSETRLFQPKDAYRMAGGGGGPLPPTATIGRNPANGVVVYY
                     SLKAKPATDLELEFLDSSGKSIRKFTQRIRREGEPSSPATPPAGEGGGFGGGGAQPTV
                     TTDAGLNRFVWDTRHSDAVRFPGMILWAGETRGPRLPPGTYQVKLTVDGKPFTQNFQI
                     KADPRVTTTAADYAKQLELGLRIRDKLSETHNAIIQIRDVRRQVEDLLKRVGSQSKPI
                     NDAGTALNKKMTAIEETLYQTKNQSNQDPLNYPIRLNNKLAALGGVVGSAEAAPTAQS
                     YAVYDELVTAIDAELQKLSQVMK"
     gene            complement(3312..4310)
                     /locus_tag="VFO72_04450"
     CDS             complement(3312..4310)
                     /locus_tag="VFO72_04450"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET9478567.1"
                     /translation="MKRIAIIGAGSWGTALAILSARAGHRVQLWSRNADVISSINEQR
                     VNSRYLTSIEIPTGVTATGELSTALDQAELVIFAGPSHAARDIFTTIAPLLGEDAIVI
                     SVSKGIEIGTGKRISEIHKELAGAARPFVCLSGPSFATEVVAGHPTAIVAASKDAAAA
                     RTVQTELSFENLRIYTNADVIGTEIGGSVKNVMAIAAGMAAGLGFGSNSVAALITRGL
                     AEITRLARREGAQMETLMGLAGLGDLVLTCTGTLSRNRFVGQELGKGRRLEEITAELH
                     EVAEGINTARAVKKLADRANLEMPIVNEVNAVLYEGKSAREAVSELMSRPLREEAH"
     gene            complement(4307..4933)
                     /gene="plsY"
                     /locus_tag="VFO72_04455"
     CDS             complement(4307..4933)
                     /gene="plsY"
                     /locus_tag="VFO72_04455"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET9478568.1"
                     /translation="MRALFVVVLAYLIGSIPFGYLIVKLTAGADIRQTGSGGTGATNV
                     SRRAGKLAGIATLLLDALKGAVAVFVAAKWSDQFQPNWIVADWIIVGSAVAVIVGHIF
                     PVWLGFRGGKGVATGAGAFFVLHPLAVLCAGVVFVIVVALTRYVSLSSITGALVIPLS
                     IWLMHVFLGSDLSLAPMLVGATVSALLIVFAHRGNIARLLSGTEPKFS"
     gene            5041..5271
                     /locus_tag="VFO72_04460"
     CDS             5041..5271
                     /locus_tag="VFO72_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HET9478569.1"
                     /translation="MRANLIEAAKGLPLPERIELLDALWESIIEEGYEPPLTKDQAEE
                     LDRRLEAHRHNPDDVISWETIQTELKAKNGSD"
     gene            complement(5597..6841)
                     /locus_tag="VFO72_04465"
     CDS             complement(5597..6841)
                     /locus_tag="VFO72_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET9478570.1"
                     /translation="MFETLTAEIIAIGSELLAPDRTDTNSLWLTEQLNRLGIEVKLKT
                     IVGDDDARLEEAIKDAVRRSKVVITTGGLGPTEDDITRKITARALGRRLLLDEEVLAE
                     IRRRFQSFGVAMPERNSRQAMVIEDAVVLPNPNGTAPGMFIDHHGTAIVLLPGPPREM
                     RPMFENHVAERLAGRTGSLRVVRRMLRVAGMGESAVDEKIAPLYTQYDNPVTTILFNQ
                     SEIEVHLTARGRTEKEANDLLDRLSAQIEERLGNAVFSFAGETMEQVVSLKLSLGGYT
                     VAVAESCTGGLLAQRLTDVPGSSKYFIEGVVTYANEAKTRTLGVEPMLLLEHGAVSAP
                     VAEAMAEGIRKRAGTDFGLSITGIAGPDGGTEEKPVGTVFIALADDVKTEHRKLHLPG
                     DRHLIRWRASQAALDFLRRRLI"
     gene            complement(6867..7601)
                     /locus_tag="VFO72_04470"
     CDS             complement(6867..7601)
                     /locus_tag="VFO72_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478571.1"
                     /translation="MTSGTCKLTTKTIAIVVIVAILNAVGVSPVVLVFVTGVVFVVWL
                     VARRAQMREVERVFEFYAAADAILREDERRWYGFEIAEVIEDGESLLEMMPDPPPLSF
                     FALGALYQRLGNHEAAVQYLSRLSDDELYNETHRIAPSPQLRRYVMMLRRLELHPAAE
                     PETLAAIRSLERSRQRSAGKMLIESRGVVESRAAAVAAKTAETPAREIKEQPFPASAP
                     LSSISAPPPITTVLRDVYQEESNASN"
BASE COUNT         1606 a         2259 c         2098 g         1713 t
ORIGIN      
        1 tcttcataac ctgcgatagc ttttgtaact ccgcgtcgat agccgtcacc agctcgtcgt
       61 aaactgcgta cgactgcgcg gtaggcgcgg cttcggcgct gcctaccaca ccaccgagcg
      121 ccgcgagctt gttgttgagc cggatcggat agttcaacgg atcctggttg ctctggttct
      181 tggtctgata gagcgtctct tcgatcgccg tcatcttttt gttcaaagca gttccagcat
      241 cgttgatcgg cttcgactga gagccgacgc gtttgagcag atcttcaacc tggcggcgca
      301 cgtcgcggat ctgaatgatg gcgttgtgtg tttcgctcaa cttgtcgcga atccttagac
      361 ccaactccaa ctgctttgcg taatccgcag ccgttgttgt cactcgcgga tcggctttga
      421 tctggaagtt ttgcgtaaac ggcttaccgt cgacagtgag cttcacctgg taagttccag
      481 gcggcagcct tggtccgcgt gtttctccag cccacaggat catccccgga aaacggaccg
      541 cgtccgaatg cctcgtgtcc caaacaaagc gatttagtcc ggcgtcagtc gtcaccgtcg
      601 gctgcgcacc gccaccacca aaaccgccgc cttcaccggc gggcggagtc gctggtgaag
      661 acggctcacc ttcacgtctg attctttgcg taaactttct aatcgatttt ccactcgaat
      721 ccaaaaactc caactctaaa tccgtggccg gtttcgcttt cagcgagtaa tagaccacca
      781 cgccgttggc cgggtttctg ccgatcgtcg ccgtgggcgg caagggacca ccgcccccac
      841 cagccattcg ataagcgtct ttcggttgaa acagccgtgt ctcgctgacc gagttaaaac
      901 cacctgcatc catcagttgg tgcagcagcg gaagatcgtc aagaatccag aacgatcgtc
      961 cctgtgtcgc caccaccaac tctttttcac gtttgtgaac tgcgagatcg gtgatcggca
     1021 cgaccggcag attaagttgc aacgactgcc agcgttcgcc gttgtcaaac gaaacgtaca
     1081 ttccggtttc cgttccagcg taaagcactc cacgtctgtt cggatcttcg cgaatgacgc
     1141 gtgtgaacgc cgtgtcggga ataccgttag tgatcttctt ccacgtctta ccgtagtcgc
     1201 tggtgcgata gagatacggc cgattgtcgt cccacttgta catggtaccc gcgagatatg
     1261 ccgtggccgg atcgtgcggc gaggcctcga gcgagttgat ctgaatccat tccggcatga
     1321 tgctcgccgg cggcgtcacg tttgaccagt tcttcccgcc gtcgcgcgtt aaatgaacga
     1381 cgccatcatc gctgcccgtc cagatggttc ccgcctgcaa cggcgactcc attacggcaa
     1441 aaatcgtcgc gtagtactcg acgctcgtat tgtctttcgt gattggacca ccggaagctg
     1501 cctgcttcga tttgtcgttg cgcgtgagat ccggactgat gatctgccaa ctgctgcctt
     1561 cgtcgtttgt tttgaatagc acgttcgccg cggagtagag cgtgttcgga tcgtgcggcg
     1621 aaaagacgat tgggaagttc cactgaaaac ggtacttcaa cacgtccgcg cccgagccca
     1681 tcgggttgtt cggataagga ctgatgttgc gctgttgacc tgtgcgatga tcgtagcgcg
     1741 tcaagagacc accgtacgaa cccgcgaaga cgatgttgga atcttttggt gagggagcga
     1801 tccagcccga ctcgccaccg ccaacgtcaa accagtcttt ctctgtgatc gagttctctg
     1861 tcgtgcggct cgcaatgcga atagttgaat tgtcctgctg cgcgccgtag atgttgtagg
     1921 ggaaatcgtt gtcgaccgtg acgcgataga actgcgccgt cggctggtcc tgctccgtcc
     1981 aactccggcc gccgttgaag gaaacattcg cgccgccgtc gttgctttcg atcatgcggt
     2041 tcggatcgtc gggcgcgatc cacagatcgt ggttgtcacc gtgtggcact gagatggtcg
     2101 tgtacgtgcg accaccgtcg ttggatttga agaaaccgac gttcagaacg taaacggaat
     2161 cggcgttttt cggatcggca tagatgcgcg tgtagtacca ggcacgttgg cgcagccgtc
     2221 gttcttcgtt gaccttggtc caggtgcggc cgccgttgtc tgaacgaaac acgccaccgt
     2281 cttctgcttc aacgatcgcc cagacgcgct cgggattcgc gggtgagaca gtgatgccga
     2341 ctttgcccac cagtccacgc ggcagtcccg gattgcgcgt cagttctgtc caggtgtcgc
     2401 cgccgtcggt cgatttgaag atgccgccgc cgggtccgcc actctcgagc gtccacggtt
     2461 tgcggtagac ctcccaaaaa cctgcgtaga tgatgcttgg attagtggga tcgagaatca
     2521 aatcgatagc gccggccttg ttgccgcggc tcaggatctt ctcccacgtc ttgccaccat
     2581 cttttgaacg aaagacgccg cgctgatcgt ttggtcccca aacgtggccg agcgccgcaa
     2641 cgtaaaccag gtccgggttc ttcggatgca cacggatgcg cgggatttgt cgtgtatctt
     2701 cgaggccgat tcgcttccac gtctttccgg catctgtaga cttgtaaact ccatcgccgt
     2761 gcgagacgtt gccgcggatc ggcgactcgc ccatgccgac gtaaaccacg tttggatcgg
     2821 aatcggaaac ggcaatcgca ccaaccgatc ccgtgccaaa cactgagcca tcactgaccg
     2881 attcccagtt gatcccgccg tcggtcgact tccaaactcc gccaccggtc gcgccgtagt
     2941 aataaacaaa cggttgcgag gcgacacccg cgactgctgc cgagcgcccg ccgcggtaag
     3001 gaccgatgtt ccgatactgc aaaggcttga gcagatcgaa cggcgccggc gtgggtgacg
     3061 gctgcggacg ctgcgcctga acagcgaccg tggaaagcac cagtgcgaca acgagacgga
     3121 gaatgacggt atggcgagac gcacggaacg tcttgaacat tcggatgagc tcctttactt
     3181 gttacttgaa aagtcgatgg cttgcggctg cgaataatat caattcccgt ttggagttca
     3241 agctacttct tctctgcgtc tctgtgcctc gggtggttaa ttgaccgcag agacacgaag
     3301 acaccgagag attaatgggc ttcttcgcgc aggggacggc tcatcaattc cgacacggcc
     3361 tcgcgtgcgg acttaccttc gtagagcacc gcgtttactt cgttcacgat gggcatttcg
     3421 aggttggcgc gatccgcaag ttttttcacc gcacgcgcag tattgatgcc ttccgcgact
     3481 tcgtgtaact cggcggtgat ctcttcgagt cgtcttcctt ttccgagttc ctgccctaca
     3541 aatcgattgc gtgagagagt tccggtgcag gtcagcacga gatcgccgag accggcgagg
     3601 cccatcaacg tttccatttg cgcgccttcg cgcctggcca gacgcgttat ctccgcgagg
     3661 ccacgagtta tcaacgccgc gacgctgttt gagccaaacc cgagaccggc agccattccc
     3721 gccgcgatcg ccatcacgtt ttttacagaa ccgccgattt cagtgccgat gacgtctgcg
     3781 ttggtgtaga tgcgcaggtt ctcgaaactg agttcagtct ggactgtgcg cgcggcagct
     3841 gcgtctttgc tggcggcaac gatcgcagtc gggtgaccgg caacgacttc tgtagcgaat
     3901 gacgggccgg aaagacagac gaacgggcga gccgctccag cgagttcttt gtggatctcc
     3961 gaaatgcgct ttccagttcc gatctcaatc ccttttgaaa cactgatgac gatggcatct
     4021 tcgcctaaca gcggagcgat cgtggtgaag atatcgcgag ctgcgtgtga aggcccggcg
     4081 aagatcacaa gctctgcctg gtccagggct gtactgagct caccggttgc ggtgactcca
     4141 gttggtattt ctatggacgt gaggtaacgc gagttaacgc gctgttcgtt aatcgacgag
     4201 atcacgtcgg cattgcgtga ccagagttgc actcgatgac cggcgcgcgc tgacaggatt
     4261 gcgagcgccg ttccccaact gcccgcgccg ataatagcga tgcgcttcat gagaacttcg
     4321 gttcagtgcc actcaataga cgcgcgatgt ttccgcgatg agcaaaaaca attagcagcg
     4381 cgctgactgt cgcgcccacc aacatcggcg caagactcag gtcacttccg agaaaaacgt
     4441 gcatcagcca aatcgagagt ggaattacga gcgcgccagt gatggaactg agcgatacat
     4501 atcgcgtaag cgcaactaca atcacgaaca caacgcccgc gcaaagcacc gccaacggat
     4561 gcaacacgaa aaacgccccc gcgcctgttg cgacaccttt gccgccacga aacccgagcc
     4621 acaccggaaa gatgtggccc acaatgacag ccaccgcaga ccctacaatg atccagtcgg
     4681 cgacgatcca gttcggttga aattgatcag accactttgc cgccacgaaa accgcgacag
     4741 cgcctttgag cgcatcgagt agaagcgtcg cgattccagc gagcttgccg gcgcgacgtg
     4801 agacgttcgt agcgcccgtt ccaccagaac ctgtctgacg aatgtccgcg ccggccgtca
     4861 gcttcacgat caagtagcca aacggaatag atccgatcag atacgcaagg acaacaacaa
     4921 acagcgcgcg cattaagtga cctggctccg caaaagttcc gcttttgagt ggaggtgaac
     4981 tataacatgc tggttgagaa tcgccttgcc cttgagttgc ggtgagctta aactgctttc
     5041 atgagagcga acctgattga agccgcaaag ggccttccgt tgccggagcg aattgaactt
     5101 ctagatgcgt tgtgggagag catcatcgaa gaaggctatg aacctccgtt gacaaaagat
     5161 caagctgagg aactggaccg tcgattggaa gcgcaccgtc ataatcccga cgacgttatc
     5221 agttgggaaa cgatccaaac cgagttgaaa gcgaaaaacg gaagcgacta atgtcacgtt
     5281 cggttgtctc cgccgcatcg ctaaatccga actcgacgaa gccatagcct ggtacgagaa
     5341 aaaagaagaa ggactcggta gaaagttcca acacgccatt tcgcaacaac tcgagagagt
     5401 tgccgcatcg ccagatcagt ttgctttcat ccgcggtcct gttcgtagag ctgtgctccg
     5461 gcgctttcct tactcaattt tgtttcgtgt gtgaagcgga ccgcgtggtt gttctagcga
     5521 tctttcacgc aagcgttctc ctcagcggct tgaagctcgt ttctaaagcg ctttagcggt
     5581 atctattctc taccggctag atcaagcgcc gtcgcagaaa gtccagtgcg gcttgggagg
     5641 cgcgccagcg gattaggtga cgatcgcccg gcaggtgcag tttgcggtgt tctgttttga
     5701 cgtcgtcggc aagggcgatg aagacggtgc caaccggctt ctcttcagta cctccgtcgg
     5761 ggccggcaat ccccgtaatt gataagccaa agtctgttcc cgcgcgttta cggatccctt
     5821 cggccatcgc ttccgccacc ggcgcactca ccgcaccatg ctcgagtaac aacatcggct
     5881 cgacaccgag cgtgcgcgtc tttgcttcgt tcgcgtaagt aacaacacct tcgatgaagt
     5941 acttcgacga tccaggaacg tcagtcaaac gttgcgctag caatccaccg gtacaactct
     6001 ccgccaccgc aaccgtgtaa ccgccaagag acaacttcag actgacgacc tgctccatcg
     6061 tttcacccgc aaacgagaac accgcgttgc ccaaacgctc ttcaatctgt gccgacaatc
     6121 gatcgagcag atcgttcgct tctttttcgg tgcggccgcg cgcggtgagg tgaacctcga
     6181 tctcgctctg gttgaaaaga attgtggtga ctggattgtc gtactgcgtg tacagcggcg
     6241 caatcttctc atccactgcc gattcgccca ttccggcgac gcgtaacatt cgccgcacca
     6301 ctcgcaaact tccagtgcgt cccgcaagtc tctcggccac gtggttctca aacatcggac
     6361 gcatctcacg cggtggacca ggcaacagaa cgatcgccgt tccgtgatga tcgataaaca
     6421 tgcccggcgc ggtgccgttc ggattcggca acacaacggc gtcttcaatc accatcgcct
     6481 gtcgcgagtt tctctccggc atcgcgacgc caaacgactg aaagcgccgg cgtatttccg
     6541 ctagcacctc ttcgtcgagc agcaaacgcc ggccgagcgc gcgcgccgtg atcttgcgag
     6601 tgatgtcgtc ttcagttgga ccaagaccgc cggtcgtgat gaccactttg gacctacgaa
     6661 cggcgtcttt gattgcttct tcgaggcggg cgtcgtcgtc gccgacgatt gttttgagtt
     6721 tgacttcgat gccgaggcgg ttgagctgtt ctgtcagcca gagactgttc gtgtcggtac
     6781 gatcgggcgc cagcagttct gatccgatcg caatgatttc ggctgttaga gtctcaaaca
     6841 attaaagctc gggggactcg gatcggttaa ttactcgcgt tcgactcttc ctgataaacg
     6901 tctcgcagca cagtcgtaat tggcggtggc gctgagatag agctcagcgg cgccgatgcg
     6961 ggaaacggtt gttccttaat ttcgcgcgca ggagtttccg cagtctttgc ggccaccgcg
     7021 gcggctcggc tttcaacgac gccgcgactc tcaatcagca tcttccccgc actccgttga
     7081 cgcgatcttt cgagacttct gatcgcggcg agcgtctccg gttccgccgc gggatgaagt
     7141 tctaaccgtc gcaacatcat gacgtagcgt cgcagttgcg gcgaaggcgc gatgcgatgc
     7201 gtttcattgt aaagctcgtc atcactaagc cgcgacaagt actgcacggc tgcttcgtga
     7261 ttacccaatc gttgatacaa cgctcccaat gcgaagaagc tgagcggcgg tgggtcgggc
     7321 atcatctcca gcaggctttc gccgtcttcg atcacttcag cgatctcaaa tccgtaccag
     7381 cgtcgctcat cctcgcggag tatcgcatca gccgcggcgt agaattcaaa gacgcgttcg
     7441 acttcgcgca tctgcgcgcg gcgcgcaaca agccaaacta cgaaaacgac tccagtcaca
     7501 aacaccagca cgaccggtga aactcccacc gcgttcagga tcgccacaat gacaacaatc
     7561 gcgatcgttt tcgtcgtgag tttgcatgta cccgaagtca taaccccctc cagttctctc
     7621 gtaggacagg cattcatgcc tgtccaggga cagactggaa agtctgtcct acttat
//
LOCUS       DASTEY010000251         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_137302, whole genome shotgun sequence.
ACCESSION   DASTEY010000251 DASTEY010000000
VERSION     DASTEY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137302"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..797)
                     /locus_tag="VFO72_04475"
     CDS             complement(156..797)
                     /locus_tag="VFO72_04475"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET9478572.1"
                     /translation="MGLENIIAEALPEVLTTRIDSLINWARKSSLWPATFGLACCAIE
                     MMNSTSSRNDLARFGSEVFRASPRQADVMIVSGRVSRKMAPVLRRIYDQMPEPKWVIS
                     MGACATSGGVFDNYAIVQGVDKIVPVDVYIPGCPPRPEMLIHAVMMLQEKVMKESVRD
                     RKDVPEEEAREAVPPGTLPVTDGSAALKEALGKGAIASRPYTIASRHERRRSS"
     gene            complement(881..1342)
                     /gene="ndhC"
                     /locus_tag="VFO72_04480"
     CDS             complement(881..1342)
                     /gene="ndhC"
                     /locus_tag="VFO72_04480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9478573.1"
                     /translation="MPFRLYDYLPIVLMFIVAAGFAVGNVLLSQFVGQRKRTRTKLMP
                     YECGKDPVGSARERFSVKFYMIAMIFILFDIEVIFLVPWAAVFKSLAGLGDSMRWFVY
                     LEMMVFVGLLLAGYIYVVKKGAFDWGERARREAEAEARALHEFEARQRPAA"
     gene            1571..>2174
                     /locus_tag="VFO72_04485"
     CDS             1571..>2174
                     /locus_tag="VFO72_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478574.1"
                     /translation="MITGFNTDIEHDGVVYHVQTEDKGLDTPIILSLVYAGGTILASK
                     RSPYADLIEQGFSDEILAERLKRQHRLICAAIHSGRIDDLKKMSRGGRETVVQEAGVS
                     AEEEPSVMESAPVAEEFEIELGPFTPQWTPPPPVEEVETTDEHGLEHGTVQEETASQL
                     EEPAPEEPVEELLPEGLLITMLDDEEFYSGEKYTLRVLITN"
BASE COUNT          501 a          637 c          588 g          448 t
ORIGIN      
        1 gccagcttcc agagttggaa ttggatttaa ttcgttgttt tccggctctg atgccatctt
       61 ggtatttttg attttcgatt gccgattgtc gattgtcgaa ccttagccgt aaagtaccaa
      121 ctgcgccttg acaatcggca attggcaatc ggcaatcaag aagaacggcg ccgctcgtgt
      181 ctcgaggcaa tcgtgtacgg cctcgacgcg atcgctcctt ttccgagcgc ttctttcaac
      241 gcggcggaac cgtcagtcac cggcagcgtc cccggcggaa ctgcctcgcg cgcttcctct
      301 tccggcacgt ctttgcggtc gcgcacgctc tccttcatca ccttttcctg aagcatcatc
      361 accgcatgaa tcaacatctc cggtcgcggc gggcagcccg gaatgtaaac gtcgaccggc
      421 acaatcttgt cgacgccctg cacgattgcg tagttgtcaa acacgccacc cgaggtcgcg
      481 cacgcgccca ttgaaatcac ccacttcggt tccggcatct ggtcgtagat tcggcgcagc
      541 accggcgcca tctttcgcga cacgcgtccc gacacgatca tcacgtcggc ttgccgcgga
      601 ctggcgcgaa acacctccga cccgaagcgc gcgagatcgt ttcgcgacga agtggaattc
      661 atcatctcga tggcgcagca ggccaaacca aacgtggccg gccagagcga cgatttgcgc
      721 gcccagttga tcaaagagtc gatccgcgtg gtcagcactt ccggtaaagc ttcggcgatg
      781 atgttttcca gtcccataaa actacccttc gtcctttgtg tctctgcgtc tctgtggttt
      841 caagaatcaa ccacagagac gcagagacac agagaaatcc ttaggctgcg ggccgctgcc
      901 gcgcttcaaa ctcgtgcaac gccctggctt ccgcttccgc ctcgcgtctg gctcgctcgc
      961 cccaatcaaa ggcgcccttc ttcactacgt agatgtaacc cgccagcagc aacccgacga
     1021 aaaccatcat ttccagataa acaaaccagc gcatcgagtc gcccagcccg gccagcgact
     1081 tgaagaccgc cgcccatggc acgaggaaga tcacctctat atcgaagagg atgaaaatca
     1141 tcgcaatcat gtagaacttc actgagaacc gttcgcgcgc ggaaccgacc gggtctttgc
     1201 cgcactcgta cggcatcaac ttggttcgcg ttcgttttcg ttggccgacg aattgggaga
     1261 gaaggacgtt gcctaccgca aaacccgcgg caacgatgaa catcaagacg atcggcaaat
     1321 agtcgtacag acgaaatggc atctcagggc caccctgcta ctcaatcttt agcttgagaa
     1381 tcattgtaca agcgtgatgc tagtcgaagt gatcaagggt gtcaagcaac gttcctgagc
     1441 gggatcgatg cagaaaaaat gctgattgac cgtgaaaaaa tgcttgtgtt accgttcagg
     1501 aaaagccgcg gccacagtgg ctttttgcgg ttgagttcaa tcgggaaaga gaacgcaagt
     1561 ccttgcaaac gtgattaccg gattcaacac cgacatcgaa catgatggcg tcgtctatca
     1621 tgtgcagaca gaggacaagg ggctcgacac accgatcatt ctctcgctcg tctatgcagg
     1681 cggcaccatt ctcgccagta aacgatctcc ctacgcagat ctgatcgagc agggcttcag
     1741 tgatgaaatc ctggcggagc gtctcaagcg ccagcaccgt ttgatctgcg ccgcgattca
     1801 ctcgggccgc atcgacgacc tgaagaagat gagccgcggc ggcagggaaa ccgtggttca
     1861 ggaagccggc gtttcggccg aagaggagcc gagtgtgatg gagtctgcgc ccgtggccga
     1921 ggagtttgag atcgagttgg gaccattcac gccgcaatgg acaccgccgc cgcctgtcga
     1981 agaagtggag accacggatg aacacggact ggagcacgga acggttcagg aagagactgc
     2041 ttcgcagctc gaggagcccg cgcctgaaga accggtcgaa gaactgctgc ccgaaggtct
     2101 gttgatcacg atgctggacg atgaggagtt ctattcaggc gagaagtaca ctttacgggt
     2161 gctgatcacc aatc
//
LOCUS       DASTEY010000252         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_21613, whole genome shotgun sequence.
ACCESSION   DASTEY010000252 DASTEY010000000
VERSION     DASTEY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21613"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFO72_04490"
     CDS             <1..208
                     /locus_tag="VFO72_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478575.1"
                     /translation="LEQGKEIEQLALAELKDFSALIDTDVYETLTLEATLNAKSQIGG
                     TAREAVNAALAAARQSLAGSSPGK"
     gene            complement(150..1322)
                     /locus_tag="VFO72_04495"
     CDS             complement(150..1322)
                     /locus_tag="VFO72_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9478576.1"
                     /translation="MKVLYIILSLAILCAGCAQRRQTDERDRSPIRIGFFADLSGPTY
                     NFGQSAVNGVLLAATKTNQLGGIDGRQIDVVIEDDRGSPEEAARLAAKLIDQDKVVAI
                     IAGGTSGNSRAAAPKAQASHIPLIAPSSTDPAVTQTGDYIFRACFVDSFQGEVMASYA
                     VNTLKAKKAAILFDLNSPYGRGLTEFFEVSFRKLGGDIVIKPSYIQGDVEFKGQLSSI
                     KAAEPDVIYIPGYYAEVALIAKQARALGLQQPLLGGDGWDVPELWQLGGDALNGSYIS
                     THYSVDDPSPAIQLFVQEYKQRYANLVPDAHAALAYDATNLLFDAMNRAGTTDGVRLR
                     DALAQTKDFDGVTGFISMDENRNAVKPAVVLKLLDASFIYQETIQPKTAEQPPAPR"
     gene            complement(1319..>2095)
                     /locus_tag="VFO72_04500"
     CDS             complement(1319..>2095)
                     /locus_tag="VFO72_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9478577.1"
                     /translation="IDDVMGLLVLAVVAGAIRAAATGAVLSLLSVGLIALKAMAFLLG
                     AIVIGHYVMPHLFKGAGRFKTGGMLLSFSLAFCFLLAWVAGLVDLAPIVGAFAAGLVL
                     DEVHFKPFHRSGEVSLDELLKPLSTVLVPIFFVIMGLRVDLRAFLRKDIIGFAIMLTI
                     AAIIGKQVCSLAVVERGLNRLAVGLGMIPRGEVGLIFAGIGTTLMLPNLEGVAEPVID
                     SGTFGAVVVMVVVTTLVTPAVLKWALSRKSQQDSQPKSKH"
BASE COUNT          500 a          613 c          560 g          422 t
ORIGIN      
        1 gcttgaacag ggaaaggaga tcgagcaact agcgctcgcg gaactgaagg atttttccgc
       61 actgattgac acggatgttt atgagacgtt gacgttggag gcgacgttga atgcgaagtc
      121 gcagatcggt ggcactgcgc gcgaagcggt taacgcggcg ctggcggctg ctcggcagtc
      181 tttggctgga tcgtctcctg gtaaatgaag ctggcgtcca gaagtttcag aacgaccgca
      241 ggcttcacgg cattgcgatt ctcgtccatg ctgataaaac ccgtcacacc gtcaaagtcc
      301 tttgtttggg ccagcgcatc gcgcaatcgt acgccgtcgg tcgtgccggc gcgattcatc
      361 gcgtcaaaga gcaaattagt cgcatcgtat gcgagtgcgg cgtgtgcgtc cggaaccaga
      421 ttggcgtatc gctgcttgta ctcctgcaca aacaactgga tcgcgggcga cggatcgtca
      481 accgagtagt gcgtcgaaat gtaagatccg ttcaaagcat cgccgcctaa ctgccaaagc
      541 tccggcacat cccagccatc accgcccagc aacggttgct gcaatccgag agcccgcgcc
      601 tgtttcgcaa tcagcgccac ctcggcgtaa taacccggaa tgtagattac gtccggctcc
      661 gcagccttta tcgaactcaa ctggcctttg aattcaacat cgccctggat gtacgacggc
      721 ttgatcacaa tgtcgccgcc gagtttacga aaactgactt caaagaactc cgtgagcccg
      781 cggccgtagg gcgaattcag atcgaaaagt atcgccgcct tcttcgcctt caaagtattg
      841 accgcataac tggccatgac ttcaccttga aacgaatcga caaagcacgc gcgaaagatg
      901 tagtcgccag tttgcgtgac cgcgggatcg gtggacgacg gggcaatcag cggtatgtgc
      961 gacgcctgtg ccttcggcgc ggctgcgcgg ctattacctg aagtgccgcc cgcgatgatc
     1021 gcaacgacct tgtcctgatc gatcagtttg gcggcgagtc gtgctgcttc ttccggactt
     1081 ccgcgatcgt cttcgatcac gacgtcgatc tggcggccgt cgatgccgcc gagttgattc
     1141 gtcttcgtgg ccgccagcag cacaccgtta accgccgact ggccgaagtt gtaggttggc
     1201 ccggagagat ctgcgaagaa accgatgcga atgggactac ggtctcgctc atctgtttgc
     1261 cgccgttgag cgcaaccggc gcacaatata gcgagactca ggatgatgta taggaccttc
     1321 aatgtttact cttgggttga ctgtcttgct gcgatttgcg cgacaaggcc cacttcagca
     1381 cggcaggggt caccagtgta gttacgacga ccatcacgac gacggcgcca aaagtaccgg
     1441 aatcaatgac tggttcagcc accccttcca ggttaggtaa catcagtgtg gtcccaatgc
     1501 ccgcgaagat taatcccacc tcaccgcgcg gaatcattcc caaaccaacg gcaagtcgat
     1561 ttaagccgcg ttcgacaact gcaagcgaac agacttgttt cccgatgatc gcggcaatag
     1621 tcagcattat ggcaaacccg attatgtcct tgcgtagaaa ggcccgtaaa tccacccgca
     1681 ggcccattat cacgaaaaag attggcacca ggacagtact cagcggcttc agcaactcat
     1741 caagcgagac ttcgccgcta cgatgaaatg gtttgaaatg gacctcgtcc agaacaagcc
     1801 ccgccgcaaa ggcgccaaca attggagcga ggtctacaag tcccgccacc cacgcaagta
     1861 gaaaacagaa cgccaacgaa aacgagagaa gcatacctcc ggtcttaaac ctgcccgcgc
     1921 ccttgaatag atggggcatt acatagtgcc cgatcacgat cgctccgagc aaaaacgcca
     1981 tcgccttcag tgcgatcaaa ccgacgctaa gcaaagagag tactgctcct gtggccgcgg
     2041 cgcgaatcgc tccggccacg actgccagca ccaatagtcc cataacatca tcgat
//
LOCUS       DASTEY010000253         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_895605, whole genome shotgun sequence.
ACCESSION   DASTEY010000253 DASTEY010000000
VERSION     DASTEY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895605"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..659)
                     /locus_tag="VFO72_04505"
     CDS             complement(180..659)
                     /locus_tag="VFO72_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478578.1"
                     /translation="MPKDAYEMSENEIRSRVVTLAFGGDERLYIAFYRKLQQGLPEGT
                     GIVLRGSVITNQRHEDGAPFDSQGRQTSDIDVTLVGSKVMEFWNSDAYYIPGLHTKPL
                     CDEDPNSAPKLNPLRESLQQLIGRPVNFQATSSLVLYSRDILFGEPYYVVVHPEEDA"
     gene            complement(727..1521)
                     /locus_tag="VFO72_04510"
     CDS             complement(727..1521)
                     /locus_tag="VFO72_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9478579.1"
                     /translation="MIRIPKQIGLLLLLLALSVSPAFARKTVRVMTYNIHVGVGVDKK
                     LDLQRIADVINRERPDLVGLQEVDRGVRRTEGKDEIAELAALTRMEYAFAPNLDYQGG
                     KYGVAILSRFPIKRTIHRRFENKREAERRGMLLVEVEFDGRPLTFVTTHLDYQFEDAR
                     LFETQQLMKHLADVKGPLIVVADLNDVPTGSSYQLMRTKFDDAWVVGRSKGDGFSYPA
                     DKPAKRIDHIFTGRGVRAKKAWVIETLASDHIPVVADVELARHTKW"
     gene            1586..>2140
                     /locus_tag="VFO72_04515"
     CDS             1586..>2140
                     /locus_tag="VFO72_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9478580.1"
                     /translation="MLPWFVMFCAAFTAGAINSIAGGGTLITFPVLIWLGLDPKVANG
                     TSTVALWPGLFGGLYGYRRELENSSPILIRLGLTSIIGGAVGAWLLILTPSPVFAYLV
                     PFLILFATLLFMCQGLINRRLRLGSLEDRPTRLWWTGAMVFQFFSAIYGGYFGAGNGI
                     LMLAAMGLLGLNELHRANGIKNFLG"
BASE COUNT          445 a          612 c          558 g          525 t
ORIGIN      
        1 atggcgtcgc gaagcccaga acggaaaggc cgtcgcttca gccaggcgct cgatcacgtc
       61 cggatagatc gcttcctgaa agacgaccag atccggttcc gcggctttga ttgtttctgc
      121 gagtgcctgt tcgcggccaa cgccaccaaa gcgtatgttg taactgagca acttcaacgt
      181 tacgcatctt cctcgggatg aactacgacg tagtacggct cgccaaacaa aatatcgcga
      241 ctgtacagaa ccaggctcga agtcgcctgg aagttcaccg gccgtccgat caactgctgc
      301 aacgattcgc gcaacggatt cagcttcggc gcgctattag gatcttcgtc acataagggt
      361 ttggtatgta gtccgggaat gtagtaggcg tcggaattcc aaaattccat caccttgcta
      421 ccgacaaggg tcacgtcgat atcgctggtt tgcctgccct gtgagtcaaa gggcgcaccg
      481 tcttcgtgac gctgattcgt tatcacgctg ccgcgcagga cgatcccggt gccttcggga
      541 agtccctgtt gcagcttacg gtagaaagca atatagaggc gttcatcacc gccgaacgca
      601 agcgtaacta cgcgtgagcg gatctcgttc tcgctcattt cgtaagcatc ttttggcata
      661 accggcccct gttcgtgtta tctgtgtata agtattcgag ggggcaatat tatcgggaga
      721 tatctcttac cacttcgtgt ggcgcgcgag ttcgacgtcg gccacgacgg gaatgtgatc
      781 cgacgccaaa gtttcgatca cccatgcctt cttcgcccgc acacctcttc cggtaaagat
      841 gtgatcgatc cgcttagcag gcttgtccgc agggtaactg aatccatcgc ctttcgaccg
      901 gcctacgacc caggcgtcgt caaacttcgt gcgcatcagt tgatacgaac tgcctgtcgg
      961 tacgtcgtta agatccgcga ctacaatcaa cggacctttc acgtccgcta agtgcttcat
     1021 caactgctgt gtctcaaaca aacgtgcatc ttcgaattga tagtcaagat gcgtcgttac
     1081 aaacgtgagc ggccttccat cgaactcaac ctcgaccagc aacatgccgc gccgttccgc
     1141 ttcacgtttg ttttcaaacc tgcgatggat tgtgcgcttg atgggaaaac gagagagaat
     1201 cgcaacgccg tacttccctc cctgataatc caggttcggc gcgaaggcat attccattcg
     1261 cgttaacgct gccagctcgg cgatctcatc cttaccttcg gttcgtctga cgccgcgatc
     1321 tacttcctgc agaccaacca gatccggacg ctcgcgattg atcacgtcag cgatccgttg
     1381 caggtccagt ttcttatcca cgccgacgcc gacgtgtatg ttgtaagtca tcacgcggac
     1441 agtcttcctg gcgaaagcgg gagagaccga aagggcgaga agaagtagaa gcaggcctat
     1501 ctgtttagga atccgaatca tccgtgacca aactcgactc aagtttttac tggcgtaaga
     1561 acgttgcgga tcttaacatg actgcgtgct cccctggttt gtcatgttct gcgccgcatt
     1621 caccgccggc gcgatcaatt ccattgccgg gggcggaact ctaattacct tccctgtcct
     1681 aatctggctc gggctcgatc cgaaagttgc aaacggtacc agcaccgtcg cgctctggcc
     1741 cggtctcttc ggtggactgt acggctaccg ccgcgaactc gagaacagtt cgccgatact
     1801 catccggctt ggtctaacca gcatcattgg cggggcagtg ggtgcgtggt tactgattct
     1861 tacaccttca cctgtctttg cttacctcgt cccgtttctc attctcttcg cgactttgct
     1921 gtttatgtgt caggggctga ttaacaggcg cctgcggctc ggatcgcttg aagaccggcc
     1981 gacaagactt tggtggaccg gcgcgatggt ctttcaattt ttttcggcga tctatggcgg
     2041 ctactttggc gcgggtaacg gcatcctgat gctcgccgcg atgggcctgc tgggtttgaa
     2101 cgaactgcat cgagcgaacg gcatcaagaa ttttctcggc
//
LOCUS       DASTEY010000254         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1439475, whole genome shotgun sequence.
ACCESSION   DASTEY010000254 DASTEY010000000
VERSION     DASTEY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439475"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..685)
                     /locus_tag="VFO72_04520"
     CDS             complement(125..685)
                     /locus_tag="VFO72_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478581.1"
                     /translation="MRTFKLLAIAFACCLTVTAQTNKTFTQGGLSFDYPSDWPLQDVS
                     NNDAQQLALTKVNSDLQIIVFVHQGRVTPEKLPDAKKAFIDPYIAARTKQLQDMGAKT
                     EQKPDSSEIGGVKADGVAINASMGGETGAAKIYWAIIGQRVVVLTYFAPDSQTKQLSP
                     AWDLIRTTLKVVDPQPAPSPSPKASP"
     gene            complement(682..>2186)
                     /locus_tag="VFO72_04525"
     CDS             complement(682..>2186)
                     /locus_tag="VFO72_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9478582.1"
                     /translation="VRKRLQSVKIAAVNSPKTHLAPELGDMSKEDFRRLGHELIDWVA
                     DYFEHIDERPVLAAIEPGDLKSKLPSVPPAEGEPMEQIIADLDRLIAPALTHWSHPSF
                     FAYFATSTSAPGILGELLTAAFDNKAMLWRTSPASTELEEVVLDWLRQMMGLDPGMHG
                     IIYDTASVSSMHAIAAAREGVEKRIREEGMSGRPDLPLLRVYVSEQAHSSIEKAVITL
                     GLGQRGLRKIPTDSEFRMDAGALADAINEDKRNGYLPFCIVATVGTTSTSSIDPVSEI
                     IPIGEEHAMWLHVDAAYAGSAAIVPELRHILAGCERADSLVTNPHKWLFTPFDLSVLY
                     CRHMDLLKRAFSLVPEYLRTPEQEKVRNGSDYGIQLGRRFRALKLWMVMRYFGHEGLA
                     ARIREHCRLARLFASWVEESPDWELMAPLPLALVCFRAKPESHRDQLDELNEQIMHRV
                     NASGRALLSHTRLNDKLTLRLSIGNIRTTEQHVRQVWELLNEQLRIAEEI"
BASE COUNT          460 a          610 c          644 g          472 t
ORIGIN      
        1 aacaacccgg gtccgagagt tttatgaatc ttcaacgcgg catctacgac gatggtcgcg
       61 atttcattct cagtcatgtg aggactcctt gtgaggttag aagataactg tctatcagtt
      121 tcgctcaggg tgacgctttg ggagaagggc tgggagcggg ttgggggtcg acgactttga
      181 gggtggtgcg gatgagatcc caggcggggg agagttgttt ggtttggcta tctggtgcga
      241 aataagtcag gacaactacg cgctgaccga tgatggccca gtagattttg gcggcgccgg
      301 tttcaccgcc cattgaggcg ttgatagcca caccgtcggc tttgacccca ccaatctctg
      361 aagagtcagg cttttgctcc gtctttgcac ccatgtcctg taattgtttc gttctggcag
      421 cgatgtaagg atcgatgaag gcttttttgg cgtccggcag cttctctggc gtgacgcggc
      481 cctggtgaac gaaaacaatg atctgcaggt cgctgttaac tttggtcaga gccaactgtt
      541 gggcgtcatt gttgctgacg tcctgaaggg gccaatcgct cggatagtcg aacgagagcc
      601 cgccctgcgt gaaagtttta tttgtttgtg ccgtcacggt caggcaacaa gcaaacgcga
      661 ttgctaacag tttgaaagtc ctcatatctc ctcggcaatc cgcaactgct cgttcagcaa
      721 ctcccacacc tgtcgcacat gctgctccgt ggtgcggatg ttaccgatcg atagtcgcaa
      781 ggtaagtttg tcgttcaggc gtgtgtggga gaggagcgcg cggccactcg cattcacgcg
      841 atgcatgatc tgttcgttta actcatccag ctgatcgcga tgcgattccg gcttcgcgcg
      901 gaaacagacg agcgccagcg gtaacggcgc catcagctcc caatcaggcg actcttccac
      961 ccacgacgca aacaaacgcg ccagccggca gtgctcgcga atgcgtgcag cgagaccttc
     1021 gtgtccgaag taacgcatca ccatccataa cttcaacgca cgaaagcgcc gcccgagttg
     1081 tatgccgtag tcagagccat tgcggacctt ttcctgttcg ggagtgcgca ggtactccgg
     1141 cacgagggag aacgcgcgct tgaggaggtc catgtgccgg caataaagca ccgacagatc
     1201 gaacggcgtg aagagccact tgtgtggatt ggtgacgagc gagtcggcgc gctcgcaacc
     1261 agcgaggatg tgccggagct caggaacaat cgccgccgag cctgcatacg ccgcatcgac
     1321 gtgcaaccac atcgcgtgct cttcaccaat gggaatgatc tcggacaccg gatcgatgct
     1381 cgaagtcgac gtcgtgccga ccgtcgccac gatgcagaac ggaagataac cattccgctt
     1441 gtcttcgttg attgcgtcgg cgagagcgcc ggcgtccatg cgaaactcgg agtcagtcgg
     1501 aatcttgcgc aggccacgtt ggcccaaccc tagcgtgatg actgctttct cgatcgacga
     1561 gtgcgcctgt tcggagacgt acacgcgcag cagtgggaga tccgggcggc cactcattcc
     1621 ttcttcacga atgcgcttct cgacaccttc gcgcgccgcg gcgatcgcgt gcatgctcga
     1681 cacagaagca gtgtcgtaga tgatcccatg catgcccgga tcgagtccca tcatttgccg
     1741 cagccagtcg agcacaactt cttccaactc cgtcgaagct ggcgaggtgc gccagagcat
     1801 cgccttgtta tcgaaggcgg ccgtcaaaag ttcaccaaga atgccgggcg ctgacgttga
     1861 agtagcgaag taagcgaaga acgatggatg gctccagtga gtcagcgccg gagcgatcag
     1921 tcgatcgaga tccgcaatga tctgttccat cggctcgcct tcagccgggg ggacggaggg
     1981 cagtttcgac ttcaagtctc caggctcaat ggccgccagc accggacgct catcgatgtg
     2041 ctcgaagtaa tcagcaaccc aatcgatcaa ctcatggcca agacgacgaa agtcttcttt
     2101 gctcatgtcg cccagttcgg gcgcgaggtg ggttttgggg ctgttcacgg cggcaatctt
     2161 aacggattga agccgcttcc gcactc
//
LOCUS       DASTEY010000255         4993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1225335, whole genome shotgun sequence.
ACCESSION   DASTEY010000255 DASTEY010000000
VERSION     DASTEY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225335"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VFO72_04530"
     CDS             <1..1443
                     /locus_tag="VFO72_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9478583.1"
                     /translation="ASLGGSYTDDVHISETSASPRGTGGKRPSVKQLESNTILNHRYE
                     IVRRIGGGGMGAVYLAKDRNLGDAPRAVKEMVESHLDPAQHEKAIGDFKRESLLLTSL
                     EHPCIPTIYDYFYDEALGRFYLVMKYISGGDLASRMRAAVGGKLDEKTVTDWGMQVAD
                     VLDYLHSRPKPIIYRDLKPANLMIDGNTGRVMLIDFGIARWVSQQEKGVTAVGTMGYA
                     PPELFSGRVQPASDVYSLGATMFHLLTGADPQDNPLLIFDFSKNPRPRQINPAISTEM
                     EQILMRAVEYKPDDRFRTAGEMRNELAVHLEKLLSGRVSYGIPAPDVGRETVQVKTVY
                     CGFCGGRIAADDVFCAHCGARQPLAVVNSSALLHTARPTAKLVVAGTTELDASFILQK
                     ESNLLGRTDPHSNIFPEIDLSRFDPETKVSRRHARIWLEGETFLVEDLGSVNGTVIND
                     SVRLAPRQPRVLDSGDKLRVGETTLHFLVG"
     gene            1495..1929
                     /locus_tag="VFO72_04535"
     CDS             1495..1929
                     /locus_tag="VFO72_04535"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET9478584.1"
                     /translation="MTRTRLHVPFPLRCGAFLIDYIVLVSLVVLGTLFARMLGGGARS
                     AGSSAETAAIALAIVVALLNLGVLPGLTGLTLGKWATGLRIQRVDSGNPGIGRALLRH
                     FVGYPLSFITLGIGFLLAAVTVHGRGLHDMIAGTVVVREGSL"
     gene            2019..3746
                     /locus_tag="VFO72_04540"
     CDS             2019..3746
                     /locus_tag="VFO72_04540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9478585.1"
                     /translation="MSELIVKYPDRTADHFPLGRLRITIGRSARNDLCIPDPFASRVH
                     AEVRREGDEYVLQDLGSANGTLYNGATVEGAVHLTSGGRIQIGETEIIFDDGSYNSSM
                     GATMITDNTTSLPEATIALASGDRTTSGLLEAIEGARTKPEEISAETTAKTKHGDLLA
                     LISKVGVTLLTPATLNETLEQVVSLVFEAVPADRCLIMMRDQGSEDLHVAVARLRDRV
                     GEVGEIRVSRNVLDEVVIRGKSVLTSDAQHDPRFASGTMVLQGVRSVLAVPLGVENKV
                     FGIIYADSPIAEGRFTEDHLKVLATLASVAAIRVENARLVEARLERERLERELALASE
                     IQQRFQPTAPPHVDGYELQGISFPCYEIGGDYYDFIEREDGRLVIALGDVSGKGTAAA
                     LLMSSLHAAIHAQSASHDSLVATISAVNRYLADNIPANRFVTLFYAELDPESGALSFL
                     NAGHNPPLIVHAAGTVEQLASGGLPLGIKPDVEYREGRTQLQPGDVLVIYSDGVTEAL
                     SPAGEEFGATRLYEVVARNIRASAAGIRDRIESSLTKFAQGTSAADDITLVIVKRQAE
                     AKLTASAGD"
     gene            3967..4716
                     /locus_tag="VFO72_04545"
     CDS             3967..4716
                     /locus_tag="VFO72_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HET9478586.1"
                     /translation="MNIDPDTGIGGAQNRFPETHQSAIVQARSTDSIVRQRAFDTILT
                     SYWKPVYKYVRLKWQAGNEDAKDLTQGFFANAFEKNHFANYDAHKASFQTFLRTCLDG
                     FVAHERKAGKRLKRGGDREHFQLDFVSAEDEFELHRAAEDLTPEDYFHREWVRGMFTL
                     AVEALRRRCEETSRTVHFRLFERYDLDEQTTSYSSLGREFGLDSTTVNNYLASVRRDF
                     RRIVLEKLREITATEDEFRNEARSLLGVDVK"
     gene            4713..>4993
                     /locus_tag="VFO72_04550"
     CDS             4713..>4993
                     /locus_tag="VFO72_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9478587.1"
                     /translation="MRWISDQRLNHLRELAVNPDFSATKYRILNELARGGMGTVYLAE
                     DTELNRQIAIKVLNSSDVTADLRQRLVREAQIIAQLEHPGIVPVHDVGTL"
BASE COUNT         1071 a         1402 c         1461 g         1059 t
ORIGIN      
        1 gcctcgctcg gcggctccta caccgacgac gttcacatct cggagaccag cgcctctccg
       61 cgcggcactg gcggcaaacg tccctctgtc aaacaactag agtcaaacac gattctgaac
      121 catcgttacg agatcgttcg ccgcatcggc ggtggcggca tgggcgccgt ctatctcgcc
      181 aaggatcgca atctcggcga cgcgccgcgc gccgtcaagg agatggttga atcgcacctc
      241 gatccggcgc aacacgaaaa agcgatcggc gacttcaaac gcgagtcgtt gctgctgact
      301 tcgctcgagc atccctgcat tccgaccatc tacgactatt tctacgacga ggcgctgggt
      361 cgtttttatc tcgtcatgaa atacatctcg ggtggcgatc tcgcttcgcg aatgcgcgcg
      421 gcagttggcg gtaagctcga tgagaagact gtgaccgatt ggggcatgca ggtcgccgac
      481 gtgctcgact atctacactc gcgtcccaaa ccgatcatct atcgcgatct gaaaccagca
      541 aacctgatga tcgacggtaa caccggtcga gtgatgctga tcgacttcgg catcgcgcgg
      601 tgggtttcac aacaggaaaa aggcgttacc gccgtcggca cgatgggcta cgcaccgccg
      661 gaacttttca gcggccgcgt gcaacctgct tcagacgttt acagtctcgg cgcgacgatg
      721 tttcatctgt tgacaggcgc tgacccgcag gacaatcctc tgctgatctt cgacttctcg
      781 aagaacccgc ggccacggca gatcaatccc gcgatctcaa cggagatgga acagattctg
      841 atgcgcgcgg tcgagtacaa gcccgatgat cgttttcgca cggcgggaga aatgcgaaac
      901 gaactcgccg ttcatctcga gaagttgctg tcgggaagag tcagctacgg aatacccgcg
      961 cccgatgtcg gcagggaaac tgttcaggta aaaacggtct actgcggttt ctgtggcggg
     1021 cgcatagcgg cggacgacgt tttctgcgcg cactgtggcg cgcgccagcc gctcgcggtc
     1081 gtcaattcga gtgcgttgtt acacacggcg cggccgacag cgaaactcgt cgtggccggc
     1141 acgaccgaac tcgatgcttc gttcatattg caaaaagaga gcaacctcct cggccgtacg
     1201 gatccacact ctaatatctt tccggagatc gacctttccc gtttcgatcc ggaaacaaaa
     1261 gtgtcgcggc gccatgcgcg catctggctt gaaggcgaaa catttttggt agaagatctt
     1321 ggctcggtga atggaactgt aatcaacgac tccgtcagac ttgcgcccag acaacctcgt
     1381 gtactcgaca gcggagacaa actgcgtgtc ggagaaacga cgttgcattt tctggtgggg
     1441 taaaagactt ctttgtcatc agtgtctaaa tacgtaggaa aaccgaaggc acggatgacg
     1501 cggacacgtc tgcatgtccc ttttcccctc cgctgcggtg ctttcctgat cgactacatt
     1561 gtgctggttt cgctggtcgt tttgggtact ctttttgcgc gtatgcttgg tggaggtgct
     1621 cgttcggcag gcagttccgc tgaaactgcg gcgattgcgc tggcgattgt cgtggcgctg
     1681 ctcaatttag gcgtgctgcc gggcctgacg ggattgacgc tgggaaaatg ggctaccggt
     1741 ttgcgcattc agcgtgtcga ttccgggaat ccgggaatcg gacgggcgct gctgcgtcat
     1801 tttgtcggtt acccgctatc gttcatcacg cttgggatcg gttttttgct ggcggcagtg
     1861 accgtgcatg ggcgcggttt gcacgatatg attgccggca ccgttgtcgt tcgtgaaggt
     1921 tcgctatagg gtacttttcg acagaatttc aagatttgca ggactgtcaa atgcctcctg
     1981 tgaattctgc aatcctgtct actccaagtt tctgaaaaat gtccgagcta atagttaaat
     2041 accccgaccg caccgccgat catttcccac tgggtcgctt gcgcatcact atcggccgtt
     2101 ccgctcgcaa cgacctctgc attccggatc cattcgcttc gcgcgtgcac gcggaagtgc
     2161 gccgcgaggg agacgagtac gttttgcagg acctcggctc cgcgaatgga acgctctaca
     2221 acggcgccac cgtcgaaggc gctgtgcacc tgacttccgg cgggcgtatt cagattggcg
     2281 aaaccgagat cattttcgac gatggctcct acaactcgag tatgggcgcc acgatgatca
     2341 ccgacaacac cacctcgctt cccgaagcga cgatcgcact cgcttcaggc gaccgcacga
     2401 cttccgggtt gctcgaagcc atcgagggcg cgcgcacgaa acccgaagag atctccgctg
     2461 agacaacggc gaagactaaa cacggcgacc tgctggcgct gatcagcaaa gtgggcgtca
     2521 cgttgctcac tcccgccacg ttgaatgaga cgctcgaaca ggtcgtctcg ttggtgtttg
     2581 aagcggtgcc ggcggatcgt tgtctgatca tgatgcgcga tcagggcagt gaagatttgc
     2641 atgtggcagt ggcgcgcttg cgagatcggg ttggtgaagt cggcgagatc cgcgtcagtc
     2701 gcaacgtgct tgacgaagtt gtgattcgcg gaaagtccgt gctgacttct gacgcgcagc
     2761 acgatccacg gtttgcgagc ggcacgatgg tcttgcaagg cgtcagatcg gttctggctg
     2821 tcccgctggg tgttgaaaac aaggtgtttg gcatcatcta cgccgactcg ccaattgccg
     2881 aaggtcgttt cacagaggac cacttgaagg tgctggcaac gcttgcttcc gtagcagcaa
     2941 tccgcgtcga gaacgcaagg ctggtcgagg cgcgtttgga acgcgaacgt ctcgaacgcg
     3001 aactcgcgct cgctagtgag attcagcagc gttttcaacc tactgcgcct ccgcacgtgg
     3061 atggttacga gcttcagggg atcagttttc cgtgttacga gatcggaggc gattattacg
     3121 acttcatcga gcgtgaagac gggcggctgg tgattgcgct cggtgacgtg tcaggcaagg
     3181 gaactgcggc cgcgttgctg atgtcgtcac tgcacgcggc gattcacgcg cagtcagcgt
     3241 cgcacgattc gctggttgca actatctccg cggttaatcg ttatctggcg gataacattc
     3301 ctgcgaatcg tttcgtcacg ttgttttatg cagagctgga tccggagtcg ggcgcgttat
     3361 cgtttttgaa cgcaggccac aatccaccgc tgatcgttca tgctgcgggg acagtcgagc
     3421 aactcgcgtc gggtggattg ccgttgggaa tcaaaccgga cgttgaatat cgtgaaggcc
     3481 ggacgcagtt gcagccgggc gacgtgctcg tgatctactc ggatggtgtg actgaggcct
     3541 tgagtccggc gggagaagag tttggcgcca cgcgtttgta cgaagttgtg gcgcgtaaca
     3601 tcagggcgtc ggctgcgggg atcagggacc gcatcgagtc gtcgttaaca aaattcgcgc
     3661 agggcacgag cgccgccgac gacatcaccc tcgtaatcgt caagcgccag gcagaagcaa
     3721 aactcaccgc cagcgccggc gactagacgg atcactaaac cacaggatat tgacgctcta
     3781 ggcggagcgt cgcaatcgca ccaaaccaac aggcggagcg ccgcaggcgc ggcaataaga
     3841 tagccagtgg caagcgcgaa gcgcgtgtcc ctgggacaca gataataaga attggaggcc
     3901 cgaagggccg acagaaccgt gggattaaag gcccccgccg tagtttgtct tcggcaaaaa
     3961 gtagtcttga atattgatcc agataccggc atcggcggcg cgcaaaatcg ctttccggaa
     4021 acgcaccaat ccgcgatcgt gcaagcgcgc agcacagaca gcatcgtgcg acagcgcgcg
     4081 ttcgacacca tccttacaag ctactggaaa cccgtttaca aatacgttcg cctcaaatgg
     4141 caggccggca acgaagacgc caaagacctg acgcagggct ttttcgcaaa cgcatttgag
     4201 aagaaccact tcgcgaatta cgacgcccac aaagcgagtt tccagacgtt tctgcgcacg
     4261 tgtctcgacg gctttgtagc acacgagcgc aaggcgggta aacgcctcaa gcgcggcgga
     4321 gaccgcgaac actttcaact ggacttcgta agtgcagaag acgagtttga gttacatcga
     4381 gcagcggagg atttgactcc cgaagattac tttcaccgcg agtgggtgcg ggggatgttt
     4441 actttggctg tggaagcgtt gcgccgccgc tgcgaggaga ccagccgcac ggtccacttt
     4501 cgactatttg aacggtacga ccttgacgag caaactactt catactcgtc tctggggcgg
     4561 gaatttggct tagactcgac aaccgtcaac aactatctgg cttcagttcg tcgcgacttt
     4621 cgccgcatcg tgctggaaaa actgcgcgag atcaccgcca ctgaagacga gtttcgcaac
     4681 gaagcgcgat cgttattggg agtcgacgtg aaatgagatg gatctcggac cagcggctaa
     4741 accaccttcg cgagctggca gtgaatcctg acttcagcgc taccaaatat cgaatcctga
     4801 atgaactcgc tcgcggcggc atgggcactg tttacctcgc cgaagacacc gaactcaacc
     4861 gccaaatcgc gatcaaagtg ctcaactctt ctgatgtgac cgcggacctg cgccaacgct
     4921 tggtccgcga agcacagatc atcgcgcagc tcgaacatcc cggcatcgtg ccggtgcacg
     4981 acgtgggcac gct
//
LOCUS       DASTEY010000256         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_928448, whole genome shotgun sequence.
ACCESSION   DASTEY010000256 DASTEY010000000
VERSION     DASTEY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928448"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFO72_04555"
     CDS             complement(<1..298)
                     /locus_tag="VFO72_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478588.1"
                     /translation="MKTERVLMSLGSILFLTLTAINASGQTIPPGKQYEDMSRAERLV
                     FVGEQARRLARELSGSEYEFTAEFEKDIQKAVTQYAVRLGKTSNERGGRSDLRPV"
     gene            423..1010
                     /locus_tag="VFO72_04560"
     CDS             423..1010
                     /locus_tag="VFO72_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9478589.1"
                     /translation="MLNFSPMKAVNADNLTALLVEWHEGDEAAVDRLMPLVYEQLRSI
                     AHRYVRRERDGHTLQTSALVNEAYLRLADQKVVWQNRSHFFAVTARVMRHILIDHARR
                     RRYAKHGGEARQVPIDEASAMSMERAAELIALEEALEDLAKLDRRKSRVVELRYFGGL
                     SLEETAEVLNISLMTVRRDWRAAKAWLYRRLKEAQ"
     gene            1007..>2386
                     /locus_tag="VFO72_04565"
     CDS             1007..>2386
                     /locus_tag="VFO72_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9478590.1"
                     /translation="MSADRWQQIENVLQEALDRPRLERALFIEDVCAGDEELKTEAST
                     LIAAYEESGDFIEQPAIEQDAYVLLSADAGREIGPYRIIERLGVGGMGEVYLAQDTRL
                     SRLVALKLLPVYFASDAERLRRFQSEARAASALNHPNILTIYEVGESGGVYFIATEFI
                     DGKTIGDLMRERELSLAEILDCVEQVASALGVAHTAGIVHRDIKPENIMRRHDGLLKI
                     LDFGIAKLLEAPDSLQPEVTGFPTHTEAGLVLGTVNYMSPEQARGLPVDERTDIWSLG
                     VVLYEMLTKRLPFSGATRMDTMVAILDREPAPLSQFASEPCLSVPLLPSIVDKCLCKD
                     VTGRYRTVAELLEDLKSAREQLAGSAIERRPTRSRLASRLSPRFVSLMAGVLVLLIIA
                     GVGGTLLYRKALRQSSASATAAGKAKATKLYAQMDERERLDFVAKQEQRISSMMGERP
                     VRLDEKAVRV"
BASE COUNT          530 a          610 c          695 g          551 t
ORIGIN      
        1 ggaccggacg taaatcagat ctcccaccgc gctcgttgga ggttttgccg aggcgcacgg
       61 cgtattgagt cacagccttc tgtatgtctt tctcaaactc cgccgtgaat tcgtattcac
      121 tgcccgaaag ctctcgtgct aggcgccgcg cttgttctcc aacaaaaaca agccgctccg
      181 cgcgcgacat atcttcatac tgtttcccgg gtggaatggt ctgaccgctg gcgtttatcg
      241 ccgtgagggt caggaaaagg atgctcccca atgacataag gacacgttca gttttcatgt
      301 ttggtatctc ctcccaacct attaagcgac taacgctgag aaaactgttc actccattag
      361 ccgtaagcga gaaaagacac cgaaagtgtt catgctaagc gaaaatattt tcgagttcat
      421 tgatgctaaa ctttagccca atgaaggcgg tgaatgcgga caatttgacc gctttgctcg
      481 tcgagtggca tgaaggagac gaagcggcag tcgatcgact tatgcctttg gtgtatgagc
      541 aactgcgcag cattgcgcat cgctacgttc gccgcgaacg tgacggtcac acgttgcaaa
      601 caagcgctct cgtaaacgag gcctatcttc gtttagccga tcagaaagta gtttggcaga
      661 atcgctcgca cttcttcgcc gtaaccgcgc gagtgatgcg tcacattctt atcgatcatg
      721 cgcggcggcg gcgttatgcc aaacacggag gtgaagcacg acaggttccg atcgacgaag
      781 cgtcagcgat gtcaatggaa cgggccgctg agctcatcgc gctggaggaa gcgctcgaag
      841 atctggctaa actggaccgg cgcaaaagtc gcgtcgtcga acttcgttat tttggtggtc
      901 tcagtctcga ggaaacggct gaggtgctca acatttctct gatgactgtg cgtcgcgact
      961 ggcgcgccgc aaaggcttgg ctctatcgac gattgaagga agcacaatga gcgcagacag
     1021 gtggcaacag attgaaaacg ttttgcagga ggcactcgat cgtccgcggc tcgagcgtgc
     1081 tttgttcatt gaggatgtct gcgcgggcga cgaagagttg aaaacagagg cctccacgtt
     1141 gattgccgct tatgaagaat ctggtgactt catcgagcag ccggcaatag aacaggatgc
     1201 atacgtgttg ttgagcgctg atgccggtcg tgagattggt ccatacagaa ttattgaacg
     1261 cctgggcgtc ggcgggatgg gtgaggttta tctggcccag gacacgaggt tgagtcgact
     1321 ggtcgcattg aaactgctgc ccgtgtattt cgcttcggac gccgagcggt tgcgccgttt
     1381 tcaaagcgaa gcccgtgccg cttcagcttt gaatcatccg aacatactga cgatttacga
     1441 agtgggcgaa agcggcggag tctatttcat cgcgaccgag tttattgacg gaaaaactat
     1501 cggcgatttg atgcgagagc gggaactgtc tttggcagaa attttggatt gcgtagagca
     1561 ggtggcgtcg gcactcggtg tcgcgcacac tgcggggatt gtgcaccgcg acatcaaacc
     1621 tgaaaacatc atgcgccgcc acgacggcct gctcaaaatt cttgacttcg gcatcgcgaa
     1681 actactggaa gctcccgact ctttgcagcc cgaagttacc ggctttccaa cacacaccga
     1741 ggcgggactg gttttgggaa ccgtcaacta catgtcgccg gagcaggcgc gtggacttcc
     1801 ggttgatgag cgcacggaca tctggagtct cggcgtggtg ctttatgaaa tgctgactaa
     1861 gcgactgccg ttcagcgggg cgacccgcat ggatacgatg gttgccatac tggaccgcga
     1921 acctgcaccg ctgtctcaat tcgcgagtga gccctgtttg tccgtaccgc tgttgccgtc
     1981 gatcgtcgac aagtgtttgt gcaaggacgt gactggtcga tatcggacag tcgccgagtt
     2041 gctcgaggat ttgaagagtg ctcgcgaaca actcgctgga tcagctatcg aaagacggcc
     2101 cacacgttcg cgattagctt cacgtttgtc gccgcgcttc gtctcgttaa tggccggcgt
     2161 gctcgtgttg ctgataatcg ccggtgttgg cggaactctt ctttaccgca aagcgctgcg
     2221 tcaatcttct gcttcggcaa cagccgcagg caaggccaaa gctacgaaac tttatgcgca
     2281 aatggacgag cgcgaacgtt tggatttcgt tgccaagcag gagcaacgga tttcaagcat
     2341 gatgggcgag cggccggtga ggcttgatga gaaggcggtc cgcgtt
//
LOCUS       DASTEY010000257         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_862574, whole genome shotgun sequence.
ACCESSION   DASTEY010000257 DASTEY010000000
VERSION     DASTEY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862574"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..928)
                     /locus_tag="VFO72_04570"
     CDS             complement(149..928)
                     /locus_tag="VFO72_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET9478591.1"
                     /translation="MKILMIGDVVAKPGRVAVLERIQDLREQHQIDLAVMNAENLAGG
                     FSVTPTLCEQLFASGIDVMTSGNHIFDKKEAIDYIRKQPRLIRPANYPPNTPGSGVWT
                     GEVNGIHVAVMNLMGRVFMPPSDDPFRVADQLLGSLQPNTSVRVVDMHAEATSEKVAM
                     GWHLDGRVSAVVGTHTHVQTADDRILPQGTAYLTDLGMTGSYAGVIGMSKSDVIARFT
                     SVTARRAEHSSGQVRICGAVIDIDEESGKARSIQRLNLAHD"
     gene            complement(999..1185)
                     /gene="ssrS"
                     /locus_tag="VFO72_04575"
     ncRNA           complement(999..1185)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFO72_04575"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1308..1697)
                     /locus_tag="VFO72_04580"
     CDS             complement(1308..1697)
                     /locus_tag="VFO72_04580"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET9478592.1"
                     /translation="MSSGFGRMINKGSMEGSTSSPTIRVEIYNQTYNIRSDGDTEYII
                     QLAEFVDSRMREISSGTLTVDSLKVAILAALHIADELHRLKNMHEQADAQLAARSAEC
                     AEMLDRLLKVRTTLEHEQGGAGKLSLG"
     gene            complement(1694..1975)
                     /locus_tag="VFO72_04585"
     CDS             complement(1694..1975)
                     /locus_tag="VFO72_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478593.1"
                     /translation="MMVGLSGLEKFSHLEDKIYRTIELTKTLRQEKENLEKELALIHR
                     DMGNVLNEKERLETQVEKLLAERETIRMKVEAMLDAVAALDPEMAEELR"
     gene            complement(2088..>3541)
                     /locus_tag="VFO72_04590"
     CDS             complement(2088..>3541)
                     /locus_tag="VFO72_04590"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9478594.1"
                     /translation="MGGEESEISSATADVLIESAYFDPHSVRRTARALGMDTEASRRF
                     ERGADCEGVLRAQERCVQLICELAGGVATEDSIDVYPQPLVPRTVGLRPQRVPELTSV
                     RVDRGEIVRILTGLGFKLVDDSRERLTVEVPSWRVDVEQEEDLVEEVARHTGFEKIGS
                     ELPPASASGEYQPSEMKQRLLRRALNAFGFDEAINFSFIEREEQFQAIPSLRGTGERQ
                     PLLKNPIIEEAAFMRGTLLSGLLSSLRHNLNQGVRDVRLFEIGRVFGAATKGELPQER
                     LALALVATGGAVEANKAQAEREVDFFDLKGALETAIDWMTLSPLSFTASVEPYLRPGQ
                     SARIELSDGTNVGSIGRLAESVAARYKFRYPVYVMELDLESLLSGPARVVQYSSLPRY
                     PSVVRDISLLLNRNIALDDVLHAVKEQRVTDFRGVKLVGTFEGGSIPSSKRSVTLRLE
                     YRSDERTLRDEEVEERHAQLTSSLLETFAAQQR"
BASE COUNT          750 a         1014 c          964 g          813 t
ORIGIN      
        1 ccggctcgga aaacgatagg tccttcgact tttcaggtcg tgggtacccg gggcagcaga
       61 cgtctcgcgt gcagtgttcc ggcagcgcca gtttcgtagc actgtaggcg agacgcctgc
      121 ggtctcagga taaacggtcg cggatttctt aatcgtgtgc gagatttagg cgctggatgc
      181 tgcgcgcttt tccgctttcc tcgtctatat cgatcactgc gccgcaaatt cgcacttgcc
      241 cgctcgagtg ctcggcgcgg cgcgcggtga ctgacgtaaa gcgcgcgatc acgtcagatt
      301 tgctcattcc aataacaccc gcgtaacttc ccgtcattcc gagatcagtc agataagctg
      361 ttccttgcgg caggattctg tcatccgcgg tctgcacgtg cgtgtgcgta cccacgacag
      421 ccgagacgcg gccatcgaga tgccatccca tcgcgacttt ttctgatgtt gcttcagcat
      481 gcatatcgac gacgcgaacg ctggtgttcg gttgcaggga tccgagcagt tgatccgcaa
      541 cgcggaatgg atcgtccgag ggcggcataa aaactcggcc catcaggttc atcacggcca
      601 cgtgtatacc gttaacctcg ccggtccaaa caccgctacc gggcgtgttt ggtggataat
      661 tcgcgggccg gatcaggcgc ggctgtttgc ggatgtagtc gatggcttcc ttcttatcga
      721 agatgtgatt gcccgaagtc atcacgtcga tgccggaggc aaacagttgt tcgcagagtg
      781 tcggcgtgac ggagaagccg cccgcgaggt tttcggcgtt catcaccgcg agatcaatct
      841 ggtgttgttc gcgcagatcc tggatgcgct cgagcacggc gacgcgccca ggctttgcaa
      901 ccacatctcc gatcatgagg attttcataa agttctattg aggggaacat aaaaaagacc
      961 gcgaatctcg attgagattg gcggctttct tggcgggagg gagatccgca tctgtcgttc
     1021 cgaagccgta attgaacctg caatgacagg tgggtgacct cttaatgacg tagcctctgg
     1081 cagttcttaa cgatgaagaa atgcactggc tagacgccct cggtctcccg aatcaacggc
     1141 gttggctcaa tcatcgtaaa tcggtgacga tcaccgcaga acgtttccct ccgccggacg
     1201 cgatattaac ataacgagca cgcactggcg aacaaacaat ccacagatta cacagacaaa
     1261 acaaagagtt agggagttct taagaatctg tgtaatctgt gggtagctca acctaaggaa
     1321 agtttgccgg cgccgccttg ttcgtgttcg agggtcgtcc ggactttgag gagacgatcg
     1381 agcatttccg cgcattccgc actgcgagcc gcgagctgcg cgtcagcctg ttcgtgcatg
     1441 ttcttgaggc gatgaagttc gtcagcaatg tgcagcgcgg cgagaatggc tactttcaaa
     1501 gagtcgaccg tgagtgttcc ggaagagatc tctctcatcc ggctgtcgac gaattctgcg
     1561 agttgaatga tgtattcggt gtcgccgtcg gagcggatat tgtaggtctg attatagatc
     1621 tcaactcgga tcgtcggact gctggtacta ccttccatac tgcctttatt gatcatgcgt
     1681 ccgaatccgg aactcatctg agttcctctg ccatttccgg atcgagagca gcgacggcat
     1741 cgagcatcgc ttcgactttc atgcgaatcg tctcgcgttc ggcgagcaac ttctcgacct
     1801 gcgtttcgag tcgctctttt tcgttcagca cgttacccat gtcgcgatgg atcagggcga
     1861 gttctttttc gaggttctct ttttcctggc gcagggtttt ggtgagttcg atggttcgat
     1921 aaatcttatc ttcgaggtgc gaaaactttt cgaggcctga cagaccgacc atcatttgtc
     1981 tcccgggttt gtgtaaactg aaacgcgaac gcgattatcc gacttcacgc ttgcgagcgc
     2041 aagactcatt ctgcaatccc gcacgattta ttttctgcgt cttaatttca gcgttgctgt
     2101 gcggcgaaag tctcgagtaa cgacgacgtc aactgcgcgt gccgctcttc aacttcttca
     2161 tcgcgcaaag tgcgttcgtc ggaacgatat tccaaacgca acgtcactga ccgcttggac
     2221 gacggaatgc ttccgccttc gaaagtgccc acgagcttca cgccgcgaaa gtcagtgacg
     2281 cgctgctctt tgacggcgtg caaaacgtcg tcgagagcga tattccggtt gagcaggaga
     2341 gagatgtcgc gcaccactga cgggtagcgc ggcaacgaag agtactgaac aactcgcgcc
     2401 ggcccactta ataatgattc gagatcgagt tccattacat agacgggata acggaacttg
     2461 tacctggcgg ccacgctttc tgcgagccgg ccgatgctgc cgacgttggt tccatcacta
     2521 agttctatgc gcgcagattg gccgggtcgg aggtacggct caacactcgc agtgaaactc
     2581 agcggactca aagtcatcca gtcgattgcg gtttccaagg ctcctttaag atcgaagaag
     2641 tcgacttcgc gttccgcctg tgctttgttt gcttcaactg caccgccagt cgcaaccagc
     2701 gccagggcca gccgttcctg cggcagttct ccctttgtgg cggcgccgaa aactcgtccg
     2761 atttcaaaca ggcgaacgtc gcgcacaccc tgattcaaat tgtgccggag cgaactcagg
     2821 agaccagaca acagcgtccc gcgcataaac gccgcctctt cgatgatcgg gtttttcagc
     2881 agcggttgac gctctccagt tccgcgcagc gacggaatcg cctgaaactg ctcctctcgc
     2941 tcgatgaagc tgaagttgat cgcctcgtca aaaccaaagg cgttcaacgc tcgtcgcaag
     3001 agacgttgct tcatctcgct cggctggtac tcacccgaag cactggccgg cggcaactcc
     3061 gaaccgatct tttcaaagcc ggtgtggcgc gcgacttctt caaccagatc ttcttcctgt
     3121 tcgacatcca cacgccacga aggcacctca accgtcaacc tttcgcgcga gtcgtcgaca
     3181 agtttgaacc ccaggccggt caggatgcga acaatttcgc cacggtcgac gcgaaccgat
     3241 gtgagttcag gcacgcgctg tggacgaaga cccactgtac gagggacaag aggctgcgga
     3301 taaacatcga tcgagtcctc cgtcgcaaca cctcctgcaa gctcgcagat tagctgcacg
     3361 caacgctcct gcgcgcgcag caccccttcg caatctgcgc cgcgctcgaa tctgcgcgac
     3421 gcctcagtat ccataccgag agcgcgcgca gtacgccgca ccgaatgcgg atcgaaatag
     3481 gcgctttcga tcagcacatc ggccgtcgca gacgagatct ccgactcttc gccgcccatg
     3541 a
//
LOCUS       DASTEY010000258         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_730621, whole genome shotgun sequence.
ACCESSION   DASTEY010000258 DASTEY010000000
VERSION     DASTEY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730621"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..787)
                     /gene="greA"
                     /locus_tag="VFO72_04595"
     CDS             complement(311..787)
                     /gene="greA"
                     /locus_tag="VFO72_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9478595.1"
                     /translation="MSQGIKEKLQGELDELETELRIHLPKEIKRALEFGDLRENSEYR
                     AALDRQNVVKARIVELRQRISEIASIDPNKVPRDKASYGSTLVLYDGEKDEEVTYRLV
                     TPEESDPAQGLISTTSPVGRSLMGKQEGDEVAVRTPAGARTFEIRQLRTLHDELEQ"
     gene            complement(800..1915)
                     /locus_tag="VFO72_04600"
     CDS             complement(800..1915)
                     /locus_tag="VFO72_04600"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9478596.1"
                     /translation="MFGTIGILTGGGDAPGLNAVIRAVVKTATCEHGMRVIGIEDGFE
                     GLLGATRTRLLSPSDVRGLLPRGGTILGTRNRGAFVERACEGTGRDVESVYQEAISNM
                     KIVGIDALVVLGGEGTLTIAAEFHKRGVPVCGVPKTIDNDLACTELTFGFVTALDIAT
                     EALDRLHTTAESHDRVMILEVMGRHAGWIALHSGIAGGADVILIPEIPFSLEVVAEKI
                     RARDASGSNFSIIVVAEGAQEIGHELVYQDKGDGKRAPRLGGIGQHLQQDLEQVTGRE
                     TRCVVLGHLQRGGRPNAFDRMLATNFGSSAVRAIASEKSGVMVALQAGDVVTVPLTEA
                     IKGIKTVPPNGQLVRTARDTGISFGAPDEASYHKGNS"
     gene            1990..2259
                     /locus_tag="VFO72_04605"
     CDS             1990..2259
                     /locus_tag="VFO72_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478597.1"
                     /translation="MEIDDDAKEIVREIGSAINESVENSTKVAEAIERLREAGYEMEL
                     TLRLEIGLRPHAGDDEAVTDGAALDLTEEDLQVLQRMKIRIDPEK"
BASE COUNT          488 a          676 c          606 g          493 t
ORIGIN      
        1 ggcgtagaag aaccagccca tcccaaaaag aagaccgcaa ccgacgttca aagagacgcc
       61 gatgacggtc agtatattag gattgatatg accgatagcg agccagcgta cgatcgcgtc
      121 aattatccgc tgagctgccc gtccgatact tgccccaaac atctgttacg ttcgtttctc
      181 ctcgcgacat tatgccctaa aagccgcggc cgggttaatt ttacacacaa aggcgcaaag
      241 atgcagggtg gaccgcgatc attactttgc gtctttgcgt ctttgcgtga aaactctcaa
      301 gaaagtatgt ttattgttcg agttcgtcat gtagcgtacg caactgccgg atctcgaaag
      361 tgcgcgcgcc ggcaggtgtt cgcactgcca cctcgtcgcc ctcctgcttg cccatcaaac
      421 tccggcccac cggcgacgtc gtcgatatca aaccttgtgc cggatcactc tcctccggcg
      481 ttaccagcct gtacgtcact tcctcgtctt tctcaccatc gtacagcacc agcgtggagc
      541 cgtacgacgc cttatcgcgc ggcactttgt tcggatctat cgacgcgatt tcactaatcc
      601 tttgtcgcag ctcgacgatg cgggctttta ccacgttttg ccggtcgagc gcggcgcggt
      661 attcggagtt ctcgcggagg tcgccaaact cgagcgcccg cttgatctct ttgggcaagt
      721 gaatgcgaag ttcggtttcc agttcgtcta gttcgccctg gagtttttct ttgattccct
      781 gtgacacggc ttcatcctct taactattcc ccttatgata actggcctcg tcaggagcgc
      841 caaaggaaat accagtatca cgtgccgtcc ggaccaactg tccgttagga ggcacagtct
      901 taatgccctt gattgcctcg gtaagcggca cggtaacgac atccccggcc tgcaaagcca
      961 ccatcacgcc gctcttttcg ctggctatgg cccgcactgc actcgagcca aaattggtcg
     1021 ccagcatgcg atcgaatgca tttggcctgc caccgcgctg gagatgtccg agcaccacgc
     1081 aacgcgtctc acgtccggta acttgttcaa gatcctgctg caggtgctgg ccgatgccac
     1141 cgagacgagg tgcacgtttt ccatcaccct tgtcttgata aaccaactcg tgcccgatct
     1201 cctgcgcgcc ttccgccacc acgataatgc tgaaattcga gccactggcg tcgcgcgcgc
     1261 gaatcttctc cgcaacgacc tcgagcgaga agggaatttc cggaatcagg atgacgtcgg
     1321 cgccacccgc gatacctgag tgaagcgcga tccagccggc gtgcctgccc atcacttcga
     1381 gaatcattac gcgatcgtgc gactcggcag tggtgtgcaa acgatcgaga gcttcggtgg
     1441 cgatatccag cgcagtgaca aagccgaacg tgagttcggt acaggccaga tcgttgtcga
     1501 tggtcttcgg cacgccgcat acaggaacgc cacgtttgtg gaactcagcg gctatcgtca
     1561 gtgttccttc cccgcccagg accacgagcg catcgatacc aacgatcttc atgttcgaga
     1621 tcgcttcctg gtagacgctt tcgacatcgc gaccagttcc ttcacacgcg cgttcgacga
     1681 aggcaccgcg attacgtgtt ccgagaattg ttccacctcg cggcagcagt ccgcgcacgt
     1741 cgctgggcga gagaaggcgg gtgcgggtcg cgccgagcag tccttcgaag ccgtcttcga
     1801 taccgatgac gcgcatgccg tgttcacaag tggcagtctt caccacagca cgaataacgg
     1861 cattgagtcc gggggcgtcg ccgccgcctg tcaggatgcc gatggttcca aacatggggg
     1921 caagtctatc attgacgatt atcaattgcc gatcctaaat gtttcgcgta gcataccaac
     1981 caaaaccaga tggaaatcga tgacgacgca aaagagatag ttcgcgagat tggctcggcc
     2041 ataaacgagt cggtggagaa ctcgaccaag gttgcggaag ccattgaacg cctgcgcgaa
     2101 gccggatatg agatggaatt gacgctccgg ctcgagatcg gactgcggcc tcacgccgga
     2161 gacgatgaag ctgtgacgga cggcgccgcc ctcgatctca ccgaagagga tcttcaggtg
     2221 ttgcagcgaa tgaagatccg gatcgatccc gaaaagtgat tag
//
LOCUS       DASTEY010000259         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1439600, whole genome shotgun sequence.
ACCESSION   DASTEY010000259 DASTEY010000000
VERSION     DASTEY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439600"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2357)
                     /gene="malQ"
                     /locus_tag="VFO72_04610"
     CDS             complement(<1..2357)
                     /gene="malQ"
                     /locus_tag="VFO72_04610"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET9478598.1"
                     /translation="MNFPRASGILLHPTSLPGSFGVGDLGPEAYKFVDFLESAGQSLW
                     QVLPLGPTGYGDSPYMCLSAFAGNTALISPERLVAEGLVDTDFATDKNQLLRSAYQNY
                     RKTTDTNLRSAFETFAQREAHWLEDYALFRALKDAHNGVAWHEWEPSLVRRTPAALAR
                     AREQLRQEIEAHMFYQFVFFEQWFTLKNYANEKGIRIVGDLPIFVALDSADVWTNPEQ
                     FKLDKNGRPLVVAGVPPDYFSSTGQLWGNPLYNWEHMEADGFKWWIERVRATLKVVDI
                     ARVDHFRGFAACWEIPGGDTTAERGQWVEAPGRELFKAIRKTLGQLPIIAEDLGVITP
                     DVVALRDEFGFPGMRILQFGFSGDAKNIDLPHNYVANVVAYTGTHDNDTTVGWFRSVA
                     GEGSTRTAEQIERERRFCLDYLNSDGEEIHWDFIRAVMASVANTAIVPLQDVLGLGTE
                     ARMNLPNSTEGNWSWRYRAGALTDEIATRLKNLTSLYGRLLLCVICVYLWPLAVAAQP
                     PQISKVDPPSWWTRSTLNPVRLLVHGQNLKGAQVRISGPGVRVVGLPKFNNRGTYAFV
                     DVAISPNALPGKRTVNVGSALASFEVLPPLKRQGRFQGFSPADVLYLIMIDRFNDGDP
                     SNNDPPQSRGIYDPKNQFYYHGGDLQGVIDRLPYLKDLGVTAIWLTPWYDNYDRLNQI
                     ELKEGKPSTGFHGYNPQDFYAVEEHFGSLDKLKELVDSAHRSGIKIIEDQVVNHTGPY
                     HPWVDDPPTPTWFNGTKTNHLKNVFQTWVLHDPRPAKQLRRETMEG"
     gene            complement(2354..>3095)
                     /locus_tag="VFO72_04615"
     CDS             complement(2354..>3095)
                     /locus_tag="VFO72_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9478599.1"
                     /translation="HRQYPKLWMVGEVFERDSAQTAFFIGGQKGWDGIDTRLDSVFDF
                     ALWNTSLNVFTNRLPVRALRDQLKYDALYPDPSKLTVLANNHDTPRFLSLEGGNTTGA
                     MLHTAFVLSVRGIPQLYYGEEIAMEGKEDPDNRRDFPVSAFEARGRKGKEQQMFDWTR
                     DWIRLRREHSSIRRGRLIDLHYDDNVYVFARQDSSETVVIGINRQDKEQEITVPFTSI
                     TPLVGKIRVIRGSNITLPPNSAVAFRAS"
BASE COUNT          671 a          888 c          851 g          685 t
ORIGIN      
        1 ccttccattg tttcgcgcct cagttgtttg gccggacgcg gatcgtgaag cacccaggtt
       61 tgaaaaacgt tcttcaggtg gtttgttttg gtgccgttga accaggtagg cgtcggagga
      121 tcatcaaccc acggatgata cggaccggtg tgattgacga cctggtcctc gatgatcttg
      181 attccggatc ggtgcgccga atcgaccagc tctttcaatt tatccaacga gccgaagtgt
      241 tcttcgacag cgtagaaatc ctgcgggttg taaccgtgga aaccggtgga tggctttcct
      301 tctttcaact cgatctggtt cagacgatcg tagttgtcgt accagggcgt caaccagatc
      361 gccgtgacgc cgagatcttt cagataaggc agacgatcga tgacgccctg gagatcgccg
      421 ccgtggtagt aaaactgatt tttcggatcg tagatcccgc gtgactgcgg cggatcgttg
      481 tttgacggat cgccgtcatt gaagcggtcg atcatgatca gatagagcac gtctgcgggc
      541 gagaagccct gaaaacggcc ctgccgtttt agcggcggaa ggacctcgaa agaagcgagc
      601 gcggagccaa cattaacagt gcgtttgccg ggcagagcgt tcggagagat tgccacatcg
      661 acaaaggcgt aagtgcctcg gttattaaat tttggaagac cgacaacgcg cacacctgga
      721 ccggagattc tcacctgtgc gccttttaag ttttggccgt ggacaagtag acgcacggga
      781 ttcagcgtgc ttctggtcca ccagcttggt ggatccacct tcgagatttg cgggggttga
      841 gcggcgacag caagaggcca cagataaaca cagatgacac acagcaggag acggccgtag
      901 aggcttgtca ggttcttgag acgggtagcg atttcgtccg ttagcgctcc ggcacgatag
      961 cgccacgacc agttaccttc cgtgctgttc ggcagattca tccgcgcctc tgtcccaagc
     1021 ccgagcacgt cctgcaaggg cacgatcgca gtgtttgcta cggatgccat gaccgcgcga
     1081 atgaagtccc agtggatctc ctcgccgtcg ctgttcagat aatcgagaca aaaccggcgc
     1141 tctcgttcga tctgttcggc cgtgcgcgtc gaaccttctc cggcaacgct tcgaaaccat
     1201 ccaactgtcg tgtcgttgtc gtgcgtgccg gtgtaagcca caacgttcgc gacatagttg
     1261 tgaggcaagt caatgttctt cgcgtcgccg ctgaaaccaa actgaagaat gcgcataccg
     1321 ggaaaaccaa actcgtcgcg caacgccacc acgtccggcg tgattacgcc caggtcttca
     1381 gcgatgatcg gcaactggcc caacgttttt cgaatcgcct tgaaaagttc tctgccgggc
     1441 gcttccaccc actgtccacg ttccgccgtc gtgtcgccgc ccggtatctc ccagcaagcg
     1501 gcgaagccac gaaagtggtc cacacgcgca atgtccacaa ccttcaatgt cgcgcgtact
     1561 cgctcgatcc accatttgaa gccgtcggct tccatatgct cccagttgta caacggattg
     1621 ccccagagtt ggcccgtgct gctgaaatag tcaggcggca cgccggcgac taccagcggg
     1681 cgtccattct tgtctagctt gaactgctcg gggttggtcc agacgtccgc cgagtccaga
     1741 gcgacgaaaa tcggcaggtc accgacaatc ctgatgccct tctcgttcgc atagttcttc
     1801 aacgtaaacc actgctcaaa gaatacgaac tggtagaaca tgtgcgcttc gatctcttgg
     1861 cgcaactgtt cgcgagcacg cgcgagcgcc gccggcgtgc gacgcacgag tgacggttcc
     1921 cattcgtgcc aggcgacacc attgtgggcg tctttcaagg cgcgaaagag cgcatagtcc
     1981 tcgagccagt gcgcttcccg ctgggcgaac gtttcgaacg cgctgcggag attcgtatcc
     2041 gtcgtcttcc tgtaattctg ataggcactc cgtaagagtt ggtttttgtc tgtcgcgaaa
     2101 tccgtgtcga ccaggccttc cgcaaccagg cgctccggac tgatgagtgc cgtgttaccg
     2161 gcaaatgccg agagacacat gtacggcgag tcgccatagc cggttggtcc aagcggtaat
     2221 acttgccaca aactctgtcc ggcgctttcg aggaaatcga cgaacttgta agcctctggt
     2281 ccaagatcgc ccacgccgaa actgcccggc aaagaagtcg ggtgcagcag aattccgctc
     2341 gctctgggaa agttcatgat gctctgaaag ctaccgccga gttaggcgga agagttatgt
     2401 tactgccacg gataacacgg atttttccca ccaatggagt gatcgatgtg aacggtacag
     2461 tgatctcttg ctccttgtcc tggcgattga tgccgataac caccgtctcg ctgctgtcct
     2521 gacgtgcgaa cacgtaaacg ttgtcatcat agtgcaggtc aatgagccga ccgcgacgga
     2581 tcgacgaatg ctctcgtcgc aaacgaatcc agtctctggt ccaatcaaac atctgctgct
     2641 cttttccctt gcgaccacgc gcttcaaagg cgctgacagg aaagtcgcgc cggttgtcag
     2701 gatcttcctt tccctccatc gcgatctctt cgccgtaata gagctgcgga ataccacgaa
     2761 cgctcaaaac gaaagcggtg tgcagcatcg ccccggtcgt gttgccacct tcgagcgaaa
     2821 ggaagcgcgg cgtgtcgtga ttgttcgcga gcactgtcag cttcgatggg tcgggataaa
     2881 gcgcgtcgta tttcaactga tcgcgcaacg cccgcaccgg aagtctgttt gtaaagacat
     2941 tgagcgaagt attccacaac gcaaagtcaa aaaccgaatc aagtcgcgta tcgatcccgt
     3001 cccagccttt ctgaccgccg ataaagaacg ccgtctgcgc tgagtcacgc tcgaagactt
     3061 cgcccaccat ccacagcttc ggatattgac gatgg
//
LOCUS       DASTEY010000260         3983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1406411, whole genome shotgun sequence.
ACCESSION   DASTEY010000260 DASTEY010000000
VERSION     DASTEY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406411"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFO72_04620"
     CDS             <1..406
                     /locus_tag="VFO72_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HET9478600.1"
                     /translation="RRVMQRTLKDAGVKPEQVGYINAHGTSTPYNDKFETRAIKDTFG
                     EHAYKLAVSSTKSMTGHLLGAAGGIEGVFCALVLNRNVLPPTINYFNPDPDCDLDYVP
                     NEPREARVDYVLSNSFGFGGTNAALLFKRYEE"
     gene            512..1285
                     /locus_tag="VFO72_04625"
     CDS             512..1285
                     /locus_tag="VFO72_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET9478601.1"
                     /translation="MKIVVMMKQVANKDAILRINKDATWIEEGDLSFEVSESDGYALE
                     EALRVREKLGGEVVVCSMGPQRVKSVIKDALARGADRAIHVAGDNLTHLSPFAAATVL
                     AGAIREEKADLVMTGLQSDDHGYGQTGVIMAELLGLPHATIVIELDAAPDKLRVKREL
                     ESGWYQWYEMSLPALLTIQSGISQIRYATLKGIMAAKKKEIKEVTPSAEVVGRPSHQR
                     IEKIYLPQKMKQTQLLGNGDAKAGAAELAQKLHTEARIF"
     gene            1313..2299
                     /locus_tag="VFO72_04630"
     CDS             1313..2299
                     /locus_tag="VFO72_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9478602.1"
                     /translation="MSNGILVFIEHKGGVANKSSLEAIAAAQTLGSQLQQTVSAVVLG
                     SDVSNLAQEIAAYDLAKVISATSPKLSDYTPDAYAGGLEQVVKQVDPSFVFLTHTYQV
                     RDFGPKLAARFGKGMISDCIRARVEGGKIGFTRRIFLGKLDADIVSDGEAPVFATFQT
                     GAYRPDQAQKGSGAPVENVAVEIGDVRMKPEAPFQEAKQAVDLTKADVIIAVGRGIKS
                     KENLALAEKLAEALGGDIAASRPICDAEWLPIDRQIGSSGQTVAPKLYVALGISGAIQ
                     HLVGMKNSGTIVAINKDPEAPIFDIADYGIVGDLFEAVPVLTEEVRKIKGLA"
     gene            complement(2283..3128)
                     /locus_tag="VFO72_04635"
     CDS             complement(2283..3128)
                     /locus_tag="VFO72_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9478603.1"
                     /translation="MSNPLRLTVLMLLTLVVAFGGCRRNEKRYDLKGKVVMVEREKHL
                     VTISHEDVEGLMPAMTMAFTVPDQTALDFLAPEDQVTATLVVDGSHSWLENLFVVRMS
                     ADSRGAPTKAEAMPGDEVPNYTLKNQDGKDIRLHNYRGKALLLTFIYTRCPLPEYCTL
                     LSNNFAQIDRQLQQNAEVYARTHLLSISIDPKFDTPQVLRSYGAAHTERYENETFSHW
                     EFASGDHVKEMAEFFGLRYFADKDQIVHGLKTIIVAPDGKVAKVYTGNEWKPEEVVSE
                     LKRVL"
     gene            complement(3125..>3983)
                     /locus_tag="VFO72_04640"
     CDS             complement(3125..>3983)
                     /locus_tag="VFO72_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9478604.1"
                     /translation="PLKRSELLATLRSAYDSLAPYRLPRFNQLSLSPPSGADPQPVHD
                     ALADALLRLGLYDEALPEFLATAAAKNTSADQAYTLARLSLQAGLPNRAVRFAEQLWK
                     NLPADYVLDLAPRDLIELLYPVPFRESLLKHAGSRGVDPRFVLSIARQESRFQVEAKS
                     VAAARGMMQFIPSTANEIAAQLNLATFQQNDLYNADTAILFGSQYLANLFKQFPDQPQ
                     AVAGSYNGGADNLARWIGRSRSTEADRYVPEIGFTQTKDYVYKVMANFWTYQRLYDAQ
                     LQPLSGTPK"
BASE COUNT          892 a         1047 c         1210 g          834 t
ORIGIN      
        1 gcgccgcgta atgcaacgaa ctttgaaaga cgcgggcgtg aaaccagaac aggttggtta
       61 tatcaacgcc cacggcacgt caacgcccta caacgacaag tttgaaacgc gggcgattaa
      121 agacacgttc ggcgagcacg cttacaagct ggccgtcagt tcgacgaagt cgatgaccgg
      181 ccacttgctc ggcgccgcgg gtggaattga aggtgtgttc tgtgcgttgg tgctcaaccg
      241 caacgtgctg ccgccgacga tcaactactt caatcccgat ccggactgcg atctcgatta
      301 cgtgccgaac gagccgcgcg aagcgcgagt cgactacgtg ctttcgaaca gttttggttt
      361 tggcggcacg aatgcagcgt tgttgttcaa gaggtatgag gagtaaagaa cttggagctt
      421 ggtgctttgt actttgttcg ctatcgaggc aattgaatag cagtgcgtcg tgagagggcg
      481 aacaaagtac aaagcacaaa gtacaaagaa catgaaaatc gtagtgatga tgaagcaggt
      541 cgcgaacaag gatgcgatcc tgcgaataaa caaagacgcc acttggatcg aagagggcga
      601 tctttcgttt gaggtgagcg agtcggacgg ctacgcgctc gaggaagcgt tgcgcgtacg
      661 cgagaagctc ggcggcgagg tggttgtctg ttcgatgggt ccgcaacgtg tgaagagtgt
      721 catcaaggac gcgctcgcac gcggcgccga tcgggccatt cacgtcgccg gcgacaactt
      781 gacgcacctt tcacccttcg ccgcggcgac ggtgttggcc ggcgccattc gcgaggaaaa
      841 ggccgacctg gtgatgaccg gcttgcaatc tgacgatcat ggttacgggc aaacaggtgt
      901 gatcatggcc gagttgcttg gtctgcctca cgcgacgata gtgatcgaac tggatgctgc
      961 gcctgacaaa cttcgggtca aacgcgaact cgaaagcggc tggtaccagt ggtacgaaat
     1021 gtcgctgccg gcgctgctca cgatccagtc cggcattagc cagattcgct atgcgacgtt
     1081 gaagggaatc atggccgcga agaaaaagga gattaaggaa gtgactcctt ccgcggaagt
     1141 cgtcggccgt ccatcgcatc agcgtattga gaagatctac ctgccgcaga agatgaagca
     1201 gacacaactg ctcggcaatg gcgatgcgaa agcgggcgct gctgagttgg cgcagaaact
     1261 acatactgag gcgcggattt tttagccagc ggacttacac agaagaacac gaatgagtaa
     1321 cggcatactg gtttttatcg aacataaagg cggcgtcgcg aacaagagtt cgctcgaagc
     1381 gatagccgca gcgcaaactt taggatctca gttgcagcag accgtaagtg ctgtcgtgct
     1441 gggctcggac gtaagcaacc tggcccaaga gatcgccgct tacgacctgg ccaaagtaat
     1501 ttctgccaca agtcctaaac tctccgacta cacgcccgat gcttacgccg gcggtctcga
     1561 acaggttgtg aagcaggtag acccgagttt tgttttctta acgcacactt accaggtgcg
     1621 tgacttcggt cctaagctcg ctgcgcgttt cggaaaaggg atgatctccg actgcattcg
     1681 cgcgcgcgta gaggggggca agatcggatt tacgcgaagg atttttctcg gaaagctcga
     1741 tgcggacatc gtgtcagacg gtgaggcgcc tgtgtttgcg acttttcaga ccggcgcgta
     1801 ccggccggat caagcgcaga agggaagtgg cgcaccggta gaaaacgtcg cagttgagat
     1861 tggcgacgtc cgcatgaaac ctgaggcgcc gtttcaggaa gcgaaacagg ccgtcgatct
     1921 cacgaaggcg gacgtcatca tcgccgtcgg ccgcgggatc aagagcaaag agaacctggc
     1981 cctggcggag aagttggccg aagcactcgg cggcgacatc gctgcttcgc gcccgatttg
     2041 cgacgccgaa tggctaccaa tcgatcgcca aatcggatcg tcgggccaaa cagtcgcgcc
     2101 gaagctttat gtggcgttgg gaatctccgg cgcgatccag caccttgtgg ggatgaaaaa
     2161 ctccggcacg atcgtggcca tcaacaaaga tccggaagca ccgatcttcg atatcgccga
     2221 ctacggaatc gtcggcgatc tgttcgaagc tgtgcccgta ttgacggaag aggtcagaaa
     2281 gattaaagga ctcgcttgag ttcgctgacg acctcttcgg gtttccactc gttgcccgta
     2341 tagaccttcg cgacctttcc gtcgggcgca acgatgatag tcttcagccc gtggacgatc
     2401 tggtctttgt ccgcgaaata ccgcaaccca aagaactctg ccatctcctt cacgtgatcg
     2461 ccggaagcaa actcccagtg tgaaaaagtt tcgttttcgt aacgttcggt gtgcgccgcg
     2521 ccgtagctgc gcagaacctg cggcgtgtca aactttggat cgatactgat gctcagcaag
     2581 tgcgtcctcg cgtagacctc ggcgttctgt tgtagttgac gatcgatctg cgcgaagttg
     2641 ttgctcagca gcgtgcagta ttcgggcagc gggcagcgcg tgtagatgaa tgtcaagagc
     2701 aacgccttgc cgcggtagtt gtgaaggcga atgtctttgc cgtcctggtt tttcagcgtg
     2761 taattcggca cttcgtcgcc tggcatcgct tcggctttcg ttggcgcgcc tcgggagtcg
     2821 gcgctcattc tgacaacgaa aaggttttcc agccacgagt gcgaaccgtc gacgaccagg
     2881 gtggccgtaa cctgatcttc tggcgcgaga aagtcgagcg ccgtctggtc agggactgta
     2941 aacgccatcg tcattgccgg catgagtccc tcgacatctt cgtgcgagat cgtgacgaga
     3001 tgtttctccc gctcgaccat cactactttg cctttaaggt cgtaacgctt ctcgttcctc
     3061 cggcagccgc cgaacgcgac caccagcgtg agcaacatca acacagtcag gcgcaaaggg
     3121 ttactcattt cggtgtgcct gagaggggtt ggagttgggc gtcgtagagc cgttgataag
     3181 tccagaagtt cgccatgact ttgtacacgt aatctttcgt ttgcgtaaaa ccgatttcgg
     3241 gaacgtagcg gtcggcttcg gtggaacgcg agcgaccgat ccagcgcgcg aggttgtcgg
     3301 cgccgccgtt gtacgaaccg gcaacagctt gcggttgatc gggaaactgt ttgaagagat
     3361 ttgccagata ctgcgaaccg aagagtattg ccgtatcggc gttgtaaaga tcgttttgct
     3421 ggaaggtggc gaggttcaac tgcgctgcaa tctcattcgc cgtcgacgga atgaactgca
     3481 tcatgccacg cgcggccgca acggatttcg cttccacctg aaagcgcgac tcctgccggg
     3541 cgatcgacaa cacgaaccga ggatcgacgc ctctgctgcc tgcatgtttt agaagcgact
     3601 cacgaaaagg caccggatac aacagttcga tcaaatcccg cggagcgaga tccagcacat
     3661 aatccgcggg aaggtttttc cagagttgtt ccgcaaaacg gacggcgcgg ttcggcaggc
     3721 cggcttgcaa cgatagtctg gcgagtgtgt aggcctgatc tgcggatgtg tttttggcgg
     3781 ctgcggttgc gagaaactcc ggcagtgcct cgtcgtaaag gccgagcctc agcaacgcgt
     3841 ccgcaagggc atcatgtact ggttggggat cggcgccgct tgggggacta agggagagct
     3901 gattgaacct cggtaggcgg tagggcgcta acgagtcgta ggcggaacgt aacgtcgcca
     3961 atagctccga gcgcttcagc ggg
//
LOCUS       DASTEY010000261         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_368199, whole genome shotgun sequence.
ACCESSION   DASTEY010000261 DASTEY010000000
VERSION     DASTEY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368199"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..784)
                     /locus_tag="VFO72_04645"
     CDS             complement(137..784)
                     /locus_tag="VFO72_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478605.1"
                     /translation="MNQKRRNGSRAIAALLLFSITQIGLATRATAIPQQFIARLTTRN
                     NQPITVNGLSAATGASIVTGATIETGADQSATVNLGSLGTLDIAPNTKLVLTYDDQGN
                     LKALIIYGCAILTAKKKTSGEIATEQGSAGKTDPAAGGVLDVCFPQGATAPQVGQGAA
                     AAAGAGAGASTAPAAAGGGGLFGLGRAATIAIFAGIGAVALTPLAFQDNPSGSQP"
     gene            complement(847..2526)
                     /locus_tag="VFO72_04650"
     CDS             complement(847..2526)
                     /locus_tag="VFO72_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478606.1"
                     /translation="MSSALICGLSCFLILATLWQDMAGSLAFQGQDIIGGAAVIFKTP
                     KRVKDLVGGASAMLAVKRPPRSGRTSDVARNNRSDRQPPATVTAAVETPAEQAENLKT
                     DGDAFYDRGQYAQALEAYQKALKLTPQDPEGHAAVGDTYLTLGRFTEAAAAYEQTVRL
                     QADNAGAYSNLGYAYDKIGKVTESINALRSAVRLNSSDAIAFNNLGATLYKAGRYQEA
                     LDAFNNAVRLAPNDADALNNLGAVLYVLEQYPRALESFQKAVAAKADSADAHYNLGNA
                     YYMTGKHREATVAYRRAIQLRGDYVEARTNLGSLLISLGDLRGAIAELQESIRLRAGN
                     PVAHNNLGYAYVKLGETLDRTEANQHLRQAVAANQEALRLKPDYVKAQNNLGAAYNKL
                     GQYKEAAELLTRAARAKPDFAEAHYNLGTALYNREQFKEAVAEFQQAVRLKQDYADAY
                     NSLGTALYRDQQFDPAIEAYKKTLALKPANAETQNNLGTVYFRTRRYKEAAASFKEAV
                     RIKPDYGEAHFNLALAYVALNDRKGALDQYNKLKKLDPKLAEQFFQQYIKK"
     gene            complement(2511..>2892)
                     /locus_tag="VFO72_04655"
     CDS             complement(2511..>2892)
                     /locus_tag="VFO72_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9478607.1"
                     /translation="LKMFAAHPILGVGLGGYWIGITAYHDASGLMTPQEAHNDYLELL
                     SSGGVIGFAIGVWFVVAVVRATRRNLLSGDMRTLRMGAIVGIVGVAAHSLVDFGLHIL
                     INAIVFLALISIATRENSDPCRLR"
BASE COUNT          584 a          834 c          793 g          681 t
ORIGIN      
        1 ttggttatat tccgagacgg gcggccacgg agtgccgccc ctacagttcg ttcgtaacgg
       61 cattaataca ccggaaagtg aaaagcgtga cgttgttgat tcaacgtcac gctttcgatc
      121 tcgaatgcgg tctcggttac ggttgggagc cgctggggtt gtcctggaac gcaagcggcg
      181 tcagcgctac tgcgccgatg cctgcgaaga tcgcgatggt ggccgctcgt ccgagaccga
      241 agagcccgcc gcctcctgcc gctgccgggg ctgtcgatgc tcctgctccc gcacctgcgg
      301 ccgccgcagc gccctgccct acttgcggcg ccgtcgcacc ctgtgggaag caaacatcca
      361 gcacaccacc cgcggccgga tcagtctttc ccgcagagcc ctgttcggta gctatctcac
      421 ccgacgtctt cttcttcgct gtcaggatcg cacacccgta aataatcaac gccttcaggt
      481 tgccctggtc gtcgtacgtg agcacgagct tcgtgtttgg tgcgatgtcg agcgtaccca
      541 gtgaaccaag attaacggtc gccgattgat ctgctccggt ctctatcgtc gcgcccgtaa
      601 cgatcgaagc gccggtagcg gcactgagac cattgacggt gatcggttgg ttgtttctgg
      661 ttgttaacct tgcgatgaac tgctgaggaa tcgcagtggc tctggtcgcg agaccaatct
      721 gggtgattga gaaaagtagt aacgccgcaa ttgctctgga accattacgc cgtttttggt
      781 tcatctaagt atcccttctc agaagaaaca acgcctaagt ctactgtcac tcaatgttta
      841 ggtcaattat ttctttatgt attgctggaa aaactgctcg gccaattttg gatcgagttt
      901 cttcaacttg ttgtactggt ccaacgcgcc tttgcgatcg ttcaacgcca cgtacgcaag
      961 cgccagattg aagtgcgcct caccgtaatc cggtttgatc ctgaccgctt ccttaaacga
     1021 cgccgctgct tctttgtaac gcctggtcct gaaatagacg gtgcccagat tgttctgggt
     1081 ctccgcattc gcaggtttca gcgcaagcgt cttcttgtac gcctctatcg cggggtcgaa
     1141 ctgctgatct ctgtaaagcg cagtgccgag actgttgtac gcgtccgcgt aatcctgctt
     1201 cagccgtaca gcttgttgaa actccgccac tgcttccttg aactgctcgc ggttataaag
     1261 cgcggtgccg aggttgtaat gcgcttcagc aaagtcaggc ttggcgcgcg cagcgcgtgt
     1321 gagaagctcc gccgcctcct tgtattggcc cagcttgttg taagccgcgc cgagattgtt
     1381 ctgcgctttc acgtaatcgg gtttgagccg caaggcttcc tgattcgctg caaccgcctg
     1441 ccgcaaatgc tggttcgctt ccgttcgatc cagcgtttcg ccaagcttca cgtacgcgta
     1501 accaagattg ttgtgcgcca ccggattgcc ggcgcgaagc ctgatcgact cctgcaactc
     1561 agcgatcgca ccgcgcagat cgccaagcga gatcaacaga ctgccgagat tcgttcgcgc
     1621 ttcgacgtaa tcgccccgca actggatcgc ccggcgataa gcgacagtcg cttcgcgatg
     1681 cttgccggtc atgtagtacg cattcccgag gttgtaatgc gcgtcggcag aatccgcttt
     1741 tgcggcgacc gctttttgaa aactctcgag cgcgcgtgga tattgttcga gcacgtacaa
     1801 caccgcgccg agattgttca acgcgtcagc atcgttgggc gcaagccgaa ctgcgttgtt
     1861 aaaagcgtca agtgcttcct gataacgccc ggccttgtac aaagtcgctc cgagattgtt
     1921 gaaagcaatc gcatcactgg agttcaagcg gacagcgctt ctcaacgcat tgatcgactc
     1981 cgttactttt ccgatcttgt cgtaagcata accgaggttg ctgtaagcgc cggcgttgtc
     2041 agcttgcaag cgaactgtct gttcgtaagc agcggcggct tccgtgaagc gaccgagtgt
     2101 caggtaagtg tcgcccactg cggcatgtcc ttcgggatcc tgcggcgtaa gcttgagtgc
     2161 tttctggtag gcttcgagcg cctgggcata ttggcctcga tcgtagaatg cgtctccatc
     2221 ggtcttgaga ttctcagctt gctcggcggg cgtttcgacc gcggcagtga cggtcgcggg
     2281 tggttgcctg tcagatctgt tgtttcgggc gacatccgaa gtacggcctg atctcggcgg
     2341 tcgcttcacc gccagcattg cagacgcgcc gccgaccaga tccttaacac gcttcggagt
     2401 cttgaatatg acagcggctc cgccaatgat gtcctggccc tgaaatgcaa gcgagccggc
     2461 catgtcttgc cagagcgtgg caagtatcaa gaaacaactt aagccgcaga ttaacgcaga
     2521 cgacatggat cagagttttc tcttgtggca atcgatatca atgcaaggaa gacaatggcg
     2581 ttgatgagta tgtgcaggcc gaaatccacg agactatgag ccgcgacgcc gacgattcca
     2641 acaatcgcgc ccatccttag tgttcgcata tctccagaca agagatttcg acgcgtcgcg
     2701 cgaacaactg ccaccacaaa ccaaacgccg attgcaaacc cgatgacgcc accgctcgag
     2761 agcagctcga gataatcgtt atgagcttcc tgaggcgtca tgagtcctga ggcgtcgtgg
     2821 taagcggtga tgccgatcca gtaaccaccg agacccacgc cgaggatcgg atgtgctgca
     2881 aacatcttca aa
//
LOCUS       DASTEY010000262         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_582406, whole genome shotgun sequence.
ACCESSION   DASTEY010000262 DASTEY010000000
VERSION     DASTEY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582406"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="VFO72_04660"
     CDS             <1..1815
                     /locus_tag="VFO72_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HET9478608.1"
                     /translation="DDNPDGDLIFNHYLRVKVFTERGRESQSKVDIPFGNLYGGEVKI
                     RDIAARTIKPDGSIVELKKEDIFERTIVKLSGLKVKARSFAMPAVEPGCIIEYRWREV
                     RVKENAHYLRLQFQRDIPVRRVQYLVKPFQYENLSFKSLTMHGQSSPWVKEKNGFYST
                     TMTNMPAVQEESRMPPEDQVKTWMLVYYAPGDKQPEPNKYWMEVGKNYYERSKSLIKP
                     NDEVKQMAATLTAEAKTDEEKLARLFEFCRTKIKNTSNDASGLTSEERAKLKDNKNPG
                     DTLKRGMGTTTDIDLLFAALANASGFDARIALSPDRGDLFFDKATPNAYFIDPRNIAV
                     NVGGTWKFFNPGYNYIPFGMLIWREEGEQALITDPKQPIWVETPMSPPEKSQIQRRAK
                     LKLTEDGTLEGEVRIEYSGHFAIERKEEIDEESETQREEGLKNELKGRMSAAEITNIK
                     IENVTDHVNPLVYTYQVRVPGYAQRTGKRLFIQPAFFQHGVAPLFATASRKHPIYFHY
                     PWSEDDHVQIELPPGYALDNADAPAPFGSGKISEYKPSLSVTSDGKMLVYKRTFYFGG
                     GNAVLFPVETYPAVKAYFDQLHKQDNHIIALKQAAASN"
     gene            1845..>2820
                     /locus_tag="VFO72_04665"
     CDS             1845..>2820
                     /locus_tag="VFO72_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HET9478609.1"
                     /translation="MKAWVIGLLAFLLFAVPAKAVAGDEAPVWVQQAAALKVPSYDKD
                     VPAVVLVNERISTVDSEGRITEVRNYAVRVLQREGRGYAIGHVVYIPDANKVKDLRAW
                     LIRPGSETKRYGKDDTVDIAGALNDVYNEYRVRSISATNDADVGSVFAFSYTREDRSV
                     FSQDDWDFQNELPVLSSRYTLTLPTGWRAEAVTFNHPNVEPRVNGTSYTWELSNLAAV
                     PREPMSPRITNLVPRLAVSYYPPANTQQLNIKTFANWSEVAAWMAELEDPQVQIDDAL
                     ARKAYELTALAKTEYEKIRAIATYVQNIQYISIQTGLGRGGGYRPHSST"
BASE COUNT          723 a          747 c          780 g          570 t
ORIGIN      
        1 gatgacaatc ccgacggcga cctcattttc aatcactatt tgcgggttaa agtctttacg
       61 gaacggggac gagaatccca aagcaaggtc gatattccgt tcggcaacct gtacggcggc
      121 gaagtaaaga tcagagacat tgctgccagg acgatcaagc ccgacggttc aattgtcgaa
      181 ttgaaaaagg aagatatttt cgagcggacc atcgtgaaat tgagcggtct gaaagtgaag
      241 gcccggtcct tcgcgatgcc ggccgtcgag ccgggctgca ttatcgagta tcgctggcgc
      301 gaagtgcgcg tgaaggagaa cgcgcattac ctccggctgc aatttcaacg agacattccc
      361 gtgcggcgcg tgcaatacct ggtaaagccg ttccaatacg aaaacctgag cttcaaatcg
      421 ctaacaatgc atggccaatc ctctccgtgg gtaaaggaga agaacggctt ctacagcacc
      481 acgatgacaa acatgcccgc cgtgcaggaa gaatcacgca tgccgcccga ggatcaggta
      541 aagacctgga tgctggttta ctacgcaccc ggcgacaagc agccagaacc aaacaagtac
      601 tggatggaag ttggcaagaa ttactacgaa aggtctaagt cgctgatcaa gcccaacgac
      661 gaagtaaagc agatggcagc cacgctcacc gcggaggcca aaacggacga agaaaagctg
      721 gcgcggttgt ttgagttttg ccgcaccaag atcaaaaaca cgagcaacga cgcttctggt
      781 ttgaccagcg aagaacgtgc aaagctgaaa gataacaaaa accctggtga cacattgaag
      841 cggggcatgg gcaccactac ggacatcgac ttgctcttcg cggcgctcgc aaatgcctca
      901 ggttttgacg cccgcattgc gctctcgcca gatcgcggag acctcttttt cgacaaggcc
      961 acgcccaatg cttactttat cgatccgcgg aatatcgcgg ttaatgttgg cggtacctgg
     1021 aagtttttca atcccggtta caactacatt cctttcggca tgttgatttg gcgggaagaa
     1081 ggcgaacagg cgttgattac tgatccgaag caacccatct gggtcgagac gccgatgtcg
     1141 ccgcccgaga aatcgcagat ccagcgccgc gcgaaactga agctcaccga ggacggcacg
     1201 ctcgagggcg aagttcgcat tgaatactcc ggccacttcg cgatcgaacg taaagaagag
     1261 atcgacgaag agtctgaaac ccagcgcgaa gaaggtctaa aaaacgagct aaagggcagg
     1321 atgagcgctg cggagatcac aaacatcaag atcgagaatg taacggacca cgtcaatccg
     1381 ctggtctaca cgtatcaagt gcgcgttcct ggttatgcgc agcgcaccgg caagcgattg
     1441 tttattcagc ctgcattctt ccagcatgga gttgcgccac tcttcgccac ggcaagtcgc
     1501 aaacatccaa tctacttcca ttacccctgg tcggaagacg atcacgtaca gatcgaactt
     1561 ccgcccggtt acgcgctcga taatgccgat gcgcccgcgc cgtttggcag tggcaagatc
     1621 tcggaataca aaccgagttt gtctgttacc tccgacggca agatgctcgt ttacaaacga
     1681 acgttctact ttggcggcgg caacgcggtg ctgtttccgg ttgagacgta tccggccgtg
     1741 aaggcgtact tcgatcaact gcacaaacag gacaatcata ttatcgcgtt gaaacaagcg
     1801 gcggcgtcta actagccacg gacaagaacg gacggagaaa caatatgaaa gcatgggtga
     1861 tcggtcttct cgcgtttttg cttttcgccg tgccggcgaa ggcagtagca ggtgacgaag
     1921 ctcctgtttg ggtgcaacaa gccgcggcgc ttaaagtgcc ttcgtacgac aaagacgtgc
     1981 cggcggtggt gctggtgaat gaaaggatct ccacagtcga ttccgagggt cgcatcaccg
     2041 aagtccgcaa ctacgccgtt cgggtcttgc agcgtgaagg gcgcgggtat gcgattggac
     2101 acgttgttta cattccagac gcgaacaaag tgaaagattt gcgcgcatgg ctgattcgtc
     2161 cgggtagcga aaccaagcgt tacggcaagg acgatacagt ggacatcgcc ggagcgctca
     2221 acgatgtcta caacgaatat cgcgtgcgaa gtatttcagc gactaacgac gcagacgtcg
     2281 gttcggtgtt cgccttcagc tatacgcgtg aagatcgttc agttttcagc caggacgact
     2341 gggattttca gaacgagctt ccggtactca gctcacgcta cacactaacg ctcccgaccg
     2401 gttggcgcgc tgaagccgtg acttttaatc atcccaatgt tgaaccgcga gtgaacggta
     2461 cgagttatac gtgggaactc tcgaatctcg cagccgttcc cagagagccg atgagtccgc
     2521 gaatcacgaa tttggtgccg cgtctcgcgg tgagctatta cccgcccgcg aacacacagc
     2581 agttgaacat caagaccttt gcgaactggt ccgaggtggc tgcgtggatg gctgagctcg
     2641 aagatccgca ggtgcagatc gacgacgcgc tggcgaggaa agcttacgaa ctaacggcgc
     2701 tggcgaagac tgagtacgaa aaaattcgcg cgattgccac ctacgttcag aatattcaat
     2761 acatctcgat tcaaaccggt ctcggtcgcg gtggtggtta tcgtccgcat tcgtcgaccg
//
LOCUS       DASTEY010000263         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_55258, whole genome shotgun sequence.
ACCESSION   DASTEY010000263 DASTEY010000000
VERSION     DASTEY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55258"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..>2067
                     /locus_tag="VFO72_04670"
     CDS             156..>2067
                     /locus_tag="VFO72_04670"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET9478610.1"
                     /translation="MKSLANLCLLLLLLCAPISAQQDLARYVDPFIGTAGHGHTYPGA
                     TRPFGMVQLSPDTRLTGWDGCSGYHYSDSIIYGFSHTHLSGTGISDYGDILLMPTAGE
                     VFLNALNSGSPEKGYASRFQHRNETASPGYYSVKLDDDNIFVELSATERAGIHRYTFP
                     RSQQANIIIDLNHRDKVLDSGLRITGGDTIVGWRRSQAWAKEQIVYFAIQFSHPFIGN
                     GIAIDDKLVPGVSEARGRNVKAYFRFHTEIAPVVTVRVGISAVDTNGAWLNLAAELGH
                     KSFEKVKDEARAAWNKELNKITVDGDSTSQLTNFYTALYHATLAPNLFMDVDRRYRGR
                     DLKIHQAENFTNYTVFSLWDTFRAAHPLYALIDRQRTRDFIKTFLVQYEQGGRLPVWE
                     LAANETDTMIGYHAVSVIADAAVKGIDGFDLQKAFEAMKQSAELRQHRSLGAYIDKGF
                     IAMEDERESVSKVLEYAYDDWCIAEVAHMLGRTDDYHRYLARAQSYKNVFDPVSGFMR
                     PRSNGNWIEPFDPREVTFAFTEANSWQYTFFAPQDISGLMRLMGGRRAFARKLDQMFA
                     ADSRTTGREQVDITGLIGQYAHGNEPSHHMAYLYNYAGEPWKTQFRVRQIMDPFYTPA
                     PEGLIGNEDCGQM"
BASE COUNT          469 a          607 c          565 g          426 t
ORIGIN      
        1 ttatcctggg accgcaggcg tctcgcctgc atgtctggca acaggctact tgcgaatcca
       61 tgcaggcgag acgcctgcgg tcccaggatc tacgcgtccc aggagccgca gggtgttacg
      121 ggccggagcg gttcgcatta tgagggctta acaacgtgaa aagtctggca aatctttgcc
      181 tcctgttact cctgttgtgc gcgccgattt cggcgcaaca ggatctcgcg cgatatgtcg
      241 atccgtttat cggcaccgcc ggtcatggcc acacataccc tggcgccacg cggccgttcg
      301 ggatggtcca actcagcccc gatacgcgac tcaccggttg ggacggctgc tccggttatc
      361 actactccga ttcgatcatc tacggtttta gccacacaca tctcagcggg accggcatct
      421 ccgattacgg cgatatcctg ctgatgccta ccgccggcga agttttcctg aacgcactta
      481 acagcggctc acccgaaaag ggttacgctt cacgttttca acatcgcaac gaaaccgcct
      541 cgcccggtta ttactcagtc aaactggacg acgacaacat cttcgtcgaa ctcagcgcca
      601 ccgagcgcgc cggaattcat cgctacacat ttccccgaag ccagcaggca aacatcatta
      661 tcgatctcaa tcatcgcgac aaagtgctcg attcaggtct gcgtatcact ggcggcgaca
      721 cgattgtcgg ctggcgccgc tcgcaagcgt gggccaaaga gcagatcgtc tattttgcga
      781 tccagttttc gcatccgttc atcggaaacg gaatcgcaat cgatgacaag cttgttcccg
      841 gcgtgagtga ggcacgcggc cggaacgtca aagcttactt tcgcttccac acggaaattg
      901 caccagtggt caccgtccga gtaggaattt ctgcggtcga tacaaacggc gcgtggctga
      961 atctcgccgc tgagttggga cacaagagct tcgagaaagt aaaagacgag gcaagagcgg
     1021 cgtggaacaa agaactcaac aagattacgg tcgacggtga cagcacctca cagctaacaa
     1081 acttctacac cgcgctctat cacgcgacgc tcgcgccgaa tctgttcatg gacgtcgacc
     1141 gccgttatcg cgggcgcgac ctgaagattc accaggccga gaacttcacc aactacacag
     1201 tcttctcgct gtgggacacg tttcgcgcgg cgcacccgct ctacgctctc atcgatcgac
     1261 aacgcacgcg cgacttcatc aaaacctttc tggtccaata cgaacaaggc gggcgtctgc
     1321 ccgtttggga actcgccgcc aacgaaaccg acacgatgat cggctatcac gctgtttctg
     1381 ttattgcaga cgcagccgta aaaggtatag acggtttcga cctgcaaaag gcgttcgaag
     1441 cgatgaagca aagcgcggaa ctgcggcaac atcgcagcct cggcgcttac atcgacaaag
     1501 gtttcattgc gatggaagac gagcgggagt ctgtttcgaa ggtgctcgag tatgcttacg
     1561 acgactggtg catcgcagag gtagcgcaca tgctcggccg cacggacgac tatcatcgtt
     1621 atctcgcgcg tgcacagtcg tacaagaacg tattcgatcc ggtttcaggt ttcatgcggc
     1681 cgcgcagtaa tggcaactgg atcgagccgt tcgatccgcg tgaggtaacg ttcgcgttta
     1741 cagaagccaa ttcgtggcaa tacacgtttt tcgcgcccca ggacataagc ggattgatgc
     1801 gtttgatggg cggccgtcgc gcgtttgctc gcaagctcga tcagatgttt gcggccgaca
     1861 gccggaccac cggacgcgag caggtggaca tcaccgggtt gattgggcaa tacgcgcatg
     1921 gtaacgaacc gagccatcac atggcgtatc tctacaacta cgccggcgag ccgtggaaga
     1981 ctcaatttcg cgtgcggcag atcatggacc cattttacac gcccgcgcct gagggattga
     2041 ttggcaacga agactgcggc cagatgt
//
LOCUS       DASTEY010000264         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_928543, whole genome shotgun sequence.
ACCESSION   DASTEY010000264 DASTEY010000000
VERSION     DASTEY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928543"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1426
                     /gene="trmFO"
                     /locus_tag="VFO72_04675"
     CDS             14..1426
                     /gene="trmFO"
                     /locus_tag="VFO72_04675"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET9478611.1"
                     /translation="MERLQAFLRRCEGLQDRSTGKTMNNEICVIGGGLAGVEAAWQAA
                     RLGAKVVLYEMRPVKQTPAHRTDKLAEIVCSNSLKSDEAGSASYLLKEELRRAGSLVM
                     EVAMATKVPAGAALAVDRHKFAELITQRIEREPRIRLVREEMQTVPDNSITIIATGPL
                     SSDSLTAEIMKLTGSDQMYFYDAIAPIVAADSIDTSIAFRAARYDKGGDDYLNCPLDE
                     QQYAAFYQALIEANSVPLQRFEETRWFEACLPIEELARRGVDTLRFGPMKPVGLRDPR
                     TGREPYAAVQLRQENLMADAYSLVGFQNHLRYGEQARVLRLIPGLQNAEFLQFGQIHR
                     NTYICSPRVLRATMQMRDRPNVFFAGQITGVEGYVESVAMGWLAGVNAARLACGRELV
                     EAPLPTATGALAHYVSQADTKNFQPVNITFALLPPLPELAKRGSRKKRERRQLQVELA
                     LKEWDEWLTQHHTQRETVSA"
     gene            1585..>2408
                     /locus_tag="VFO72_04680"
     CDS             1585..>2408
                     /locus_tag="VFO72_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478612.1"
                     /translation="MSGEVDENDIVRISSNLVPIPVSVIDKRGNAVLNLTLEDFELRV
                     DGQVRPLSELSRSETSVRLAMLFDNSGSLDAAREFEKQAAVRFFRKVMRKRDEAAIYS
                     IETDSYLVQPLTSDVLRLEQTIAMFGRPEGGTSLFDAIVGASSYLRPYSGRRVLVIVS
                     DGIETTSQHTDFDFVVHRVLADDCQVYVVQTGLYDGANLRALAAERRMEQLTSQTGGA
                     VYLPKTTAELDIAFDQIAADLAHQYVLSYYPGPENRDGRLHLLDLRLKTRNDVRVRS"
BASE COUNT          524 a          689 c          691 g          504 t
ORIGIN      
        1 ataagacgat cgagtggagc gactacaagc atttctccgg cgatgtgaag gactacaaga
       61 tcgaagcacc ggcaaaacca tgaataatga gatctgtgta atcggtggcg gtttagccgg
      121 cgttgaggcg gcttggcaag cggcgaggct cggcgcgaag gtcgtgcttt acgaaatgcg
      181 tccagtcaaa caaacgcccg cacaccgcac tgacaaactc gcagagattg tctgttctaa
      241 ctcgctgaaa tccgacgaag ccggctctgc ctcatatctg ttgaaagaag aactgcgccg
      301 cgccggctcg ctcgtcatgg aagtggcgat ggccaccaaa gttcccgccg gcgcagcgct
      361 cgccgtcgac cggcataagt ttgcagaact gataactcag cgcatcgagc gcgagccacg
      421 catccgcctc gttcgcgagg aaatgcaaac tgttccggac aactccatca cgatcatcgc
      481 caccggcccg ctgtcttccg actccctcac ggctgagatc atgaagctga ccggcagcga
      541 tcaaatgtac ttctacgacg cgatcgcgcc gatcgtggct gccgattcaa tcgacacgtc
      601 aatcgcgttt cgcgccgctc gttacgacaa gggtggcgac gactacctca actgcccgtt
      661 agacgagcaa cagtacgctg cgttttacca ggcattaata gaggccaaca gcgtcccttt
      721 gcagcgtttc gaagagacgc gctggtttga agcctgtctg ccgatcgagg aactcgcgcg
      781 ccgcggcgtc gacacgctgc gcttcggacc gatgaaaccg gtcggattac gcgatccgcg
      841 aaccggtcgc gagccgtacg ccgcagtgca actgcgtcag gaaaatttaa tggccgacgc
      901 atacagcctc gtaggcttcc agaatcatct acgctacggc gaacaagcgc gagtgttgcg
      961 gctgatccct ggcttgcaga atgcggagtt tctgcagttt ggacagatcc atcgcaacac
     1021 gtatatttgt tctcctcgcg tgctgcgcgc gacgatgcag atgcgcgacc ggccgaatgt
     1081 cttcttcgcc ggacagatca cgggcgttga aggttacgtt gaatcggtgg cgatgggctg
     1141 gctggccggt gtgaatgcag ctcggctggc gtgcggacgc gaactcgtcg aagccccact
     1201 gcctacggcc acgggcgctt tggcgcatta cgtctcgcag gcagatacaa agaactttca
     1261 accggtaaac atcactttcg cgctcttgcc tccgctccct gaacttgcaa agcgcggctc
     1321 gcgtaagaaa cgtgaacgca gacaactgca ggtcgagttg gcattgaaag aatgggacga
     1381 gtggctcact caacatcaca ctcaaagaga aacggtctcc gcttagcaat gctcgcggcg
     1441 gcggttgttc tgtgtctcat tggtgccctg cctgtgttga gccagtccgg acggcgtaag
     1501 gatggaaaag tcaagccccc tggtccagtt accgatccaa atcgcccggg atcaccgagt
     1561 tcaccttcca ctccatcgcg aacagtgagc ggcgaagtag atgaaaacga catcgttcgc
     1621 atcagttcca acctcgtccc gattccggtt tcggtcatcg ataaacgcgg caacgcggtg
     1681 ctcaatctga cgttggaaga tttcgaactt cgcgtcgatg gacaggtgcg gccgctcagc
     1741 gaattgtcgc gttccgagac gagcgtgcgg ttggcgatgc tttttgataa cagcggcagt
     1801 ctggatgctg cgcgcgagtt tgagaagcag gccgcggtcc ggttctttcg gaaggtgatg
     1861 cgcaaacgag atgaggccgc gatctactcg attgagacag acagctatct ggtccaaccg
     1921 ttaacttccg acgtgttgcg attggaacag actatcgcga tgtttggcag accagagggc
     1981 ggcacgtcgt tgtttgatgc aatcgtcggt gcgtcgtctt atttacgtcc ttactccggc
     2041 cgccgcgtgt tggtgatcgt ctcagacgga attgaaacga cgagtcaaca caccgatttc
     2101 gatttcgtgg tgcaccgcgt actcgccgat gattgccagg tctacgtggt ccaaaccggt
     2161 ttatacgacg gcgctaacct tcgcgcgtta gctgccgagc gacgcatgga acagttgacc
     2221 tcacagaccg gcggcgcagt ctacctgccg aagacaacag ctgaactcga tatcgctttc
     2281 gaccagattg ccgccgatct cgcgcaccag tacgtgttga gttattaccc gggtccggag
     2341 aatcgcgacg gccgtttgca cctgttggat ctgcgactca agacgcgaaa tgatgttcgc
     2401 gtgcgctc
//
LOCUS       DASTEY010000265         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_55287, whole genome shotgun sequence.
ACCESSION   DASTEY010000265 DASTEY010000000
VERSION     DASTEY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55287"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFO72_04685"
     CDS             <1..352
                     /locus_tag="VFO72_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9478613.1"
                     /translation="AWRYEHPQRKFPFYSSAAVTDSRVVLGGRDKLVHGLTLNGKAAW
                     TFTTRARVESSPAIAGGRVFVGSNDGRFYVLSLTNGAKLWEFNAGAPISASPAIANGR
                     IVIGSQDGRLYCFG"
     gene            474..1310
                     /locus_tag="VFO72_04690"
     CDS             474..1310
                     /locus_tag="VFO72_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9478614.1"
                     /translation="MPKINANGITLNYDQQGSGEPLILIPYLSADNACYAFQVAEYAK
                     HFTCISIDLRGAGESDKPDGPFSTELYADDIAAFMQAAGIARAHVTGLSLGAGIGMWL
                     AAKYPDKVKTLSVHSGWTKTDPYAKTVVEGWQVIARALNSVPETVIRGMFPWCFTPNL
                     YANRYDYVQTLADFVRSRPPMSVDGFIRQSDAVIGHDVESQLTRITAPALITFGRHDQ
                     ITSLRFAEPLTNGIKNSELVIFEDASHAPIYEVTEEFNQKTLDFLQRHSGEERLSQSG
                     AI"
     gene            complement(1303..1740)
                     /locus_tag="VFO72_04695"
     CDS             complement(1303..1740)
                     /locus_tag="VFO72_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET9478615.1"
                     /translation="MKRLLLVLVAIASLYSYAEAQTSKRRAVKTGTPNGIFSPAIIAG
                     DLVFTSGHIGVDPKTGQMAEGMEAQLEQVFRNLTAVLEASGSSIDHVVKATVFLADMN
                     DYNRMNELYRKQFKSDPPARSTVQVARLPRDARIEIEVVAVVK"
     gene            complement(1737..>3327)
                     /locus_tag="VFO72_04700"
     CDS             complement(1737..>3327)
                     /locus_tag="VFO72_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET9478616.1"
                     /translation="VLYIGIFAFRRSSGREKAEDYFLASRSLGPFVFLFSLFGTNMTA
                     FAILGSSGHAFNNGIVTFGLMASSSGLVIPLTIFLIGTRVWALGKKYGFMTPVQMFRD
                     RWECSHIGTVIFAVQAVLLIPYIIIGIMGGGTTLYTISGGVVPFWFGGAIVAMVVMSY
                     VFFGGMRGTAWVNTFQTVLFLCFGAIALIVIGVGMGGFGTAAQGLQNSPLSYLLTRER
                     ISPYYFFSYTFIPLSAIAFPHILIFCLTARKMNQFRKTVIFYPICILAIWLPCVFLGV
                     MANRVADVPQIRAKQEARRVLATQGRNMEPEKRDELREQSAGDDVIMLLLQRYAPLWL
                     AGLLGAGIMAAVMASDSQILALSTMFTEDVFAFYGGKRRFGEAVQVLTGRIFIILITL
                     FAYAVALRAPETIFELAIQYAFSGFAALSPLLIGALFWRGSTKWGALAVAVWTAFAVA
                     AVAMFQEVVPAATPGTTNVIWSFGDTEVLARTPGGTAVFKFSPVVPMVIGSALLMWLV
                     SLATQKPARSTLARYFGGENQ"
BASE COUNT          802 a          976 c          894 g          655 t
ORIGIN      
        1 tgcctggcgc tacgaacacc cgcaacgtaa gtttccgttc tactcttccg ccgcagtaac
       61 agactcccga gttgttctcg gcgggcgcga caagctggtc cacggattga cactgaatgg
      121 aaaagcggcc tggactttca caacgcgcgc gcgtgtcgaa tcgtccccgg caatcgcagg
      181 aggtcgcgtg tttgtcggat cgaacgatgg acgcttctac gtcttgagtc ttacaaacgg
      241 cgccaagctt tgggaattca acgcgggcgc cccgatatct gcatcccccg ccatcgcaaa
      301 cgggcgaatc gtcatcggtt ctcaagacgg tcgcctctat tgcttcggtt gaaccgagac
      361 aggatttcag aactagcaag atgtctgtga gcaggtaaag acctcctgcc tttcaataca
      421 aaggcggtca tcctgttagt cttgtcaatc ctgtcaacaa aggaggaaac ccaatgccta
      481 aaatcaacgc caatggcatt accctgaact acgaccagca gggcagcggt gaaccgctga
      541 tcctgatccc gtatctctcc gccgacaatg cctgttacgc gtttcaggtc gccgaatatg
      601 ccaaacattt cacctgcatc tcgatcgact tgcgcggcgc aggcgagagc gacaagccgg
      661 atggtccgtt ctcaacagag ctatatgccg acgacatcgc tgcgttcatg caggcagcag
      721 gaatcgcgcg cgcgcatgtc actggtctct cgctcggcgc cgggatcggt atgtggctgg
      781 ccgccaagta cccggacaaa gtgaagacgt tatcggtgca ctcgggctgg actaaaaccg
      841 atccctacgc gaagacggtc gtcgaaggtt ggcaggtaat cgctcgagct ctgaacagcg
      901 ttccggagac ggtgatacga ggaatgttcc cgtggtgctt cacgccgaac ctgtacgcga
      961 acaggtacga ctatgtccag acgctggcgg atttcgtgcg cagccggccg ccaatgtctg
     1021 tcgacggatt catccggcaa tcagatgccg tgatcgggca cgatgtcgag tcacaactca
     1081 cgcgtatcac agcaccggca ctgatcactt ttggccgtca cgatcagatt acttcgctcc
     1141 gctttgcgga accgctcaca aacggaatca agaattcgga gttggtgatc tttgaagacg
     1201 cttcgcacgc gccgatctac gaggtcaccg aagagttcaa tcagaagacg cttgatttcc
     1261 ttcagcgcca ttccggtgag gaaagattgt cgcagtcagg agctatttaa caacggctac
     1321 gacttcgatt tcgatgcgcg catctcgcgg aagccgcgcg acctgcacag ttgaacgagc
     1381 cggaggatcg ctcttgaact gcttgcgata aagctcgttc atcctgttgt agtcgttcat
     1441 gtccgccaag aaaacggtgg ccttcacgac gtggtcgata ctgctgcctg acgcttcaag
     1501 cacggcagtg aggttgcgaa acacctgttc gagctgtgct tccatgcctt ccgccatctg
     1561 tccggtcttt ggatcgacgc cgatatggcc tgacgtgaag accaggtcgc ccgcgatgat
     1621 cgctggagag aagatgccgt tgggtgttcc agtcttcacg gcccggcgct ttgaggtctg
     1681 cgcctctgca tagctgtaca aagaagcgat cgctacaagt acgagtagta atctcttcat
     1741 tggttttcac ctccaaaata tcttgccaac gtactccgcg ccggcttttg tgtagccagt
     1801 gaaactaacc acatcagcaa ggctgacccg attaccatcg gcacaacagg cgagaattta
     1861 aaaacagccg tcccgccagg cgtgcgtgcc aacacttctg tgtccccaaa cgaccagatc
     1921 acattggtgg ttccaggcgt cgctgcgggc accacttcct gaaacatcgc aactgccgca
     1981 acagcgaacg ccgtccagac agctactgcg agcgcgcccc actttgtact tcccctccag
     2041 aacaacgcgc cgatcaacag tggcgacaac gccgcgaaac ctgagaatgc atattgaatc
     2101 gcgagttcaa agatcgtttc tggcgcgcgc aaggcgaccg cgtaagcaaa gagcgtgatc
     2161 aggatgatga aaatgcgtcc ggtgagcact tgcaccgctt caccgaagcg acgtttgccg
     2221 ccgtagaagg cgaagacgtc ctccgtaaac atcgtcgaga gcgcgagtat ctgagagtca
     2281 cttgccatca cggccgccat gatcccggcg ccaagtagcc cggcgagcca caacggcgcg
     2341 tagcgctgca agagcaacat aatgacatcg tcgccggctg actgttctcg cagttcgtcg
     2401 cgcttctccg gctccatgtt gcggccctga gttgccagca ctctgcgtgc ttcctgcttg
     2461 gccctgatct gcggcacgtc cgcaacgcga ttcgccatca cgccgagaaa cacacacggc
     2521 aaccagatcg cgagaataca gatcgggtag aagatcacag ttttcctaaa ctggttcatc
     2581 ttcctcgccg tcaaacagaa gatcaggatg tgtggaaagg cgatcgccga caacgggatg
     2641 aacgtgtagc tgaagaagta gtaaggcgag attcgttcgc gagtcagcag atacgacagc
     2701 ggtgagtttt gcaggccctg cgcagcggtg ccaaagccgc ccatgccgac gcctatcacg
     2761 atcagggcga tcgcgccgaa gcacaaaaaa agtaccgtct gaaacgtgtt gacccatgcc
     2821 gtgccgcgca taccgccgaa aaagacgtaa ctcatcacga ccatcgctac gattgcgccg
     2881 ccaaaccaaa acggcaccac gccgccactg atcgtgtaga gagttgtgcc gccgcccata
     2941 ataccgatga tgatgtacgg aataagcagc acggcctgga ccgcaaagat cactgtgccg
     3001 atgtggctgc actcccaacg atcgcgaaac atctgcacgg gcgtcataaa gccgtacttc
     3061 ttaccgagcg cccagacacg cgtaccgatc agaaaaatcg tcaacgggat cacgaggccc
     3121 gacgacgaag ccatcaagcc aaacgtcacg atgccgttgt taaaggcgtg acccgaagaa
     3181 ccaaggatcg caaacgcggt catgttcgtg ccgaagagtg aaaacagaaa cacgaatggg
     3241 ccaagcgaac ggctggcgag aaagtagtct tcagccttct cgcgtccgct tgaacggcga
     3301 aaagcaaaga taccgatata gagaact
//
LOCUS       DASTEY010000266         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_697948, whole genome shotgun sequence.
ACCESSION   DASTEY010000266 DASTEY010000000
VERSION     DASTEY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697948"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFO72_04705"
     CDS             complement(<1..926)
                     /locus_tag="VFO72_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9478617.1"
                     /translation="MSVKNTNEEILVVDDDEAIRWTLREALQSWGFVPVEAATVAEAV
                     KHFKADLPAAVLLDIDLPDGSGLDVLREIKREHPQAVVIMITGNVQIDNTISALRGGA
                     YDFIAKPINLEELRVTLRNAIEARHLRREVEQVRKERAREFNFRQIVGESPAMKKLLS
                     LAAKVAESEVSSVLLQGESGTGKDLVAKAIHYGSQRAERPFVAVNCAALPATLIESEL
                     FGYEKGAFTDAKARKEGLFEQAEGGTLLLDEIGELELSLQAKLLRVLEEGAFRRVGGL
                     KDIPLDVRVLAASNRDLKAESEAGRFRLDLYYR"
     gene            complement(958..1845)
                     /locus_tag="VFO72_04710"
     CDS             complement(958..1845)
                     /locus_tag="VFO72_04710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9478618.1"
                     /translation="MERKVASDYPQELLDLFHEYQHGDISRRTFLDRAAKFAVGGLTV
                     ATIFEGLRPNYAWAQQVAPDDKRIKLGYETVQSAEGNGSIKGYLARPAKSGKLPVVLV
                     IHENRGLNPYIEDVARRLALADFIAFAPDGLTSVGGYPGSDEKGAEAFRKVDGKKMTE
                     DFVAAAKWLKARSDSSGKLGAVGFCFGGGMVNQLAVRLGGDLNAGVAFYGRQASVDDV
                     PKISAPLLLQYAGNDQRINAGIAAYEAALKANNKVYQVHMYEGKQHGFHNDTTPRYDE
                     EAAKLAWSRTLEFFNKHLR"
     gene            2194..>2753
                     /locus_tag="VFO72_04715"
     CDS             2194..>2753
                     /locus_tag="VFO72_04715"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9478619.1"
                     /translation="MRKAGLPRLRVTGVAVFVAIVAACAVAPKGQVRQGSPEPNQQVS
                     PEVTQQTKEVKKDVPYVPTPQNVVDEMLKLAKVTKDDVVYDLGCGDGRLVITAVKKFN
                     AKRGFGVDIDPQRITESNANAKAAGVTDRVEFAVQDLFQTDLKDATVVTLYLLPEVNL
                     RLRPKLLNELQPGTRVVSHSFDMGDWT"
BASE COUNT          588 a          807 c          754 g          604 t
ORIGIN      
        1 cgatagtaaa gatcgagtcg aaagcgtccg gcctcgctct ccgctttcaa atcgcgatta
       61 gaggcggcga gtacgcgcac gtcgagcgga atgtctttga gtccaccgac acgtcgaaaa
      121 gctccctcct ccaacacgcg caagagtttc gcttggagtg aaagttccag ctcaccgatc
      181 tcgtcaagca agagcgttcc gccttctgcc tgttcgaata acccttcttt gcgcgcttta
      241 gcatcggtaa acgcaccctt ttcataaccg aagagttccg actcaatgag cgtcgccgga
      301 agtgcggcgc agttcacagc cacaaaagga cgttcggctc gttgggagcc gtaatggatt
      361 gctttcgcga cgaggtcctt gcccgttccc gactcgccct ggagtaaaac tgacgacact
      421 tcgctttcag cgaccttcgc ggcaagactc agcagctttt tcatcgccgg agactcgccc
      481 acaatctgcc ggaaattaaa ctcgcgcgct cgttcttttc ttacttgttc tacttcacgg
      541 cgcaagtgtc gtgcttcgat tgcattacgc agtgtgacgc ggagttcttc gagattaatc
      601 ggcttggcga tgaaatcgta ggcgccgccg cgcagagctg aaatcgtgtt atcgatctga
      661 acgttgccgg tgatcatgat cacgaccgct tgcggatgtt cgcgcttaat ctcgcgcaag
      721 acgtcgagac cggaaccgtc aggcaaatcg atgtcgagta agactgccgc ggggagatct
      781 gctttgaaat gctttacggc ttctgcaacg gtggccgctt cgacaggcac gaatccccaa
      841 ctttgtaggg cttcgcgcaa cgtccagcga attgcctcgt catcatcgac gacgagtatt
      901 tcttcgtttg tatttttcac agacatggat atttgcgcga ctcactgccg ctcagagtta
      961 ccgcaggtgt ttattgaaaa actcgagcgt acgcgaccac gcgagtttcg cggcctcttc
     1021 gtcataacgc ggtgtcgtgt cgttgtggaa gccgtgctgt ttaccctcgt acatgtgaac
     1081 ctgatacacc ttgttgttgg ccttgagcgc tgcctcatat gcggcgatgc ctgcgttgat
     1141 tcgctgatcg ttgcccgcgt attgtagcaa cagcggcgcg gaaatctttg ggacatcatc
     1201 gacgctcgcc tggcgtccgt agaacgcaac gccggcgttc aaatcaccgc ccagccgcac
     1261 ggcgagttgg ttcaccattc cgcctccgaa acagaaaccg acagcgccca gtttgccgct
     1321 ggaatcggag cgcgccttga gccacttggc ggccgcgacg aagtcttcgg tcatcttctt
     1381 tccgtccact ttgcggaagg cttcggcgcc tttttcgtcg ctgcctggat agccgcccac
     1441 agaggttaaa ccatccggcg caaaagctat gaagtcggcg agtgctagcc gccgcgctac
     1501 gtcttcaatg taaggattta gcccgcggtt ctcgtggatg acgagcacaa ccggcagctt
     1561 cccactcttt gcgggccgcg cgagataacc cttgatcgag ccgttccctt ccgcggactg
     1621 cacggtttca taacccagtt tgatgcgttt gtcgtccggc gcgacttgct gcgcccatgc
     1681 atagttcggc ctgaggccct caaaaatcgt cgctacggtc aggccaccga ctgcaaactt
     1741 cgcggcgcga tcgaggaacg tgcgccggct gatgtcgccg tgctgatatt cgtgaaagag
     1801 gtccaacaac tcctgcgggt aatcactggc tactttacgt tccattcttt ctccttttat
     1861 ctgtggcggt ccttaactga tatcacgact cacacaaacc aaacaaccgg ttgggtggcg
     1921 atgcagtttg aactggggcc gattcccggg acgcgctctt ccctgggacc gcaggcgtct
     1981 cgcctgcatg tcacgagagc gcccgattcg tcaccgggca ggcgagacgc ctgcggtccc
     2041 aggaaagagc gcacccaatt aaagagtcag agcccaccac ctctcgggaa caaaaaacgt
     2101 aaagaaaaag catgaggaac gcacagaact gttcccctga cggcgctaac atccgatccc
     2161 cttcgtgcgt caggtctgaa aggagagatg aggatgcgga aagcaggatt acctcggctg
     2221 cgcgttactg gtgtggccgt ttttgttgcc atcgtcgccg cgtgcgcagt tgcgccaaaa
     2281 ggccaggtca gacaagggtc gccagaaccg aaccaacagg tgagtcctga ggtcacccag
     2341 cagaccaaag aggtcaagaa agatgttcct tacgtgccca cgccgcagaa cgtcgtggac
     2401 gagatgctca aactggcgaa agtcacaaag gacgacgtgg tctatgacct tgggtgcggc
     2461 gacgggcgac tggtgatcac cgctgtgaag aagttcaacg cgaagcgtgg ctttggagtt
     2521 gacatcgacc cgcagcgcat aacggagagc aacgccaacg cgaaagcggc aggcgtcacc
     2581 gaccgcgtcg agttcgctgt tcaggacctc ttccaaaccg atttgaagga cgctacggtc
     2641 gtcacgcttt acctcctacc agaagtgaac ctcagactgc gccccaagct gcttaatgaa
     2701 ctgcagcccg gaacacgcgt ggtttcgcac tccttcgaca tgggtgactg gac
//
LOCUS       DASTEY010000267         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1208794, whole genome shotgun sequence.
ACCESSION   DASTEY010000267 DASTEY010000000
VERSION     DASTEY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208794"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFO72_04720"
     CDS             <1..989
                     /locus_tag="VFO72_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HET9478620.1"
                     /translation="RRVNEQLQTDYHEIMLKPSVAELLPKLVYHMEEPPIDMAIPSYL
                     ISSAARETLTVMLSGMGGDEVFAGYPRQMAMKIASAFDPVPNLLRRPLMKTVAGVLPG
                     GLPGKLTAPLRNAKKFARSAALDFEDRYLGYGTYFTNEMKRRLYSDEWRARTSQYDPY
                     ATHRAYFARAAEAAPLNQLLYVDLKTFLPCLNLLTTDKTSMAANLEVRVPFLNREMIE
                     MAARMPPQLKLRGLKRKYVLKRALESVLPADVVWRKKAGFGAPIRSWLRGALRPMIGD
                     LLSEEAVKRRGIFRPKEVRRVVDANLSGREDYNLQVFQLLGLELWHQTFIHK"
     gene            1139..1549
                     /locus_tag="VFO72_04725"
     CDS             1139..1549
                     /locus_tag="VFO72_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478621.1"
                     /translation="MIRQDTLEITPFLAEAPDGAQCVLLVEDDPALRRYLEVVLQRAG
                     YDVMTAGDGLEAMKVLLTLQVDVIVTDALMPNLDGYELCRFVRNSDHLAHLPIILLSA
                     LDPKNSTDESEQVNAFLAKPVSPEELLATIISCC"
     gene            complement(1533..>2421)
                     /gene="ald"
                     /locus_tag="VFO72_04730"
     CDS             complement(1533..>2421)
                     /gene="ald"
                     /locus_tag="VFO72_04730"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9478622.1"
                     /translation="PEYPRMREGQLLFTYLHLAPDRELTKQLIERKVTGIAYETITDR
                     RGTLPLLTPMSEVAGRMAIQVGAHYLEKMSGGRGILLGGVPGVPSARVVIIGGGVVGT
                     NAAKIAVGMSAQVTIIDRDLDRLRELDDIFLSKISTLASSAYMIHDAISQADLIVGAV
                     LVPGAAAPKLVTRAMLKDVPNGAVIVDVAVDQGGCVETSHPTTHSDPTYYVEGVLHYC
                     VANMPGAVPRTSTFALTNATLPYALKLANKGFLDAIARDAGLKEGVNTYAGHCTYEAV
                     ATAQGIPYTPLDSLISNTK"
BASE COUNT          509 a          698 c          706 g          508 t
ORIGIN      
        1 cgcgtcgcgt caacgagcag ttgcagactg actatcacga gatcatgttg aagccttcgg
       61 ttgcggagct gttgccgaag ctggtctatc acatggaaga gccgccgatc gacatggcga
      121 tcccgagtta tctcatctca agcgcggcgc gcgaaacgct aacggtgatg ctctccggca
      181 tgggcggcga cgaggtgttc gccggttatc cgcggcagat ggcgatgaag attgcatccg
      241 cgttcgatcc agtgcctaac ctgctgcggc gacccttgat gaagactgtg gccggcgttt
      301 tacccggtgg actgccaggt aaactaacgg cgccactgcg caacgcgaag aagtttgcgc
      361 gcagcgcggc gctcgatttt gaagatcgtt atctcggcta cggcacgtac tttaccaatg
      421 agatgaagcg gcggctttac tcggatgagt ggcgcgcgcg cacgagccaa tacgatcctt
      481 acgcgacgca tcgtgcttac tttgcacgcg cggcagaagc tgctcctttg aatcaactcc
      541 tctacgtaga cctgaagacg tttctcccgt gcctcaatct cctgacgaca gacaagacct
      601 cgatggcggc aaatctggag gtgcgagtgc cgtttctcaa tcgcgagatg atcgagatgg
      661 cggcgcgcat gccaccgcaa ctgaaactac gtggactcaa gcgcaagtat gtcctgaagc
      721 gcgccctcga aagcgtcctg cctgcagacg tggtctggcg aaagaaggcg ggattcggag
      781 cgccgatccg gtcctggctg cggggcgccc tgcggccgat gatcggcgat ttgctgtccg
      841 aagaggcggt gaagcggcga ggcatcttcc gcccaaagga ggtccggcgc gtggtggacg
      901 cgaacctttc gggccgggaa gactacaacc tccaggtttt ccagctttta ggacttgagc
      961 tctggcacca aacctttatc cataaatgac ttagccgcag ctatacgcgg atgtacgcag
     1021 gtcagctttg cgtccatcta cgtttatcag tagccaaaat tgtttgacaa ccatgcgtct
     1081 cgcgggctac cctgctccgt gagagtcacc ccggctcggt cctcttcact cacgcaccat
     1141 gatcagacaa gacacactcg aaattacccc gtttttggcg gaagcaccgg acggggctca
     1201 gtgtgtgctt ttggtcgagg acgatccggc actgcggcgc tacctcgaag tcgtgcttca
     1261 gcgcgccgga tacgacgtca tgactgccgg cgacgggctg gaggccatga aggttctgct
     1321 cactttgcag gtcgacgtta ttgttactga tgctttaatg ccgaatcttg acggatacga
     1381 gttgtgccgt ttcgtcagga acagcgatca cctggcacac ttgccgataa tcctgctcag
     1441 cgccctcgat ccgaaaaatt cgactgatga gtctgagcag gtaaatgcgt tcctggcgaa
     1501 accagtctca cctgaagaac tgctggcgac aattatttcg tgttgctgat tagcgaatct
     1561 agcggcgtat acggaatgcc ctgggcggtg gcgacagcct cgtaggtgca gtggccggcg
     1621 tatgtgttga cgccttcttt cagaccggcg tcacgtgcga tcgcatcaag gaaacctttg
     1681 ttagcgagct tcaaagcgta gggcagcgtt gcgttggtca acgcgaacgt ggaagtgcgc
     1741 gggacagcgc ccggcatgtt agcgacgcag tagtgcaaca cgccttcgac gtagtacgtc
     1801 ggatccgagt gtgtggtcgg atgcgaggtc tcgacacagc cgccctgatc cacggcaacg
     1861 tcaacgataa ccgcgccgtt cggcacgtct ttcaacattg cgcgggtaac gagctttggc
     1921 gcggctgcac caggcacaag cactgctccc acgatcagat cggcttgcga gatcgcgtca
     1981 tggatcatgt aagcgctgga cgcgagcgtc gagatcttcg acaggaaaat gtcatcgagc
     2041 tcgcgcaatc gatcgaggtc acgatcaatg atcgttactt gcgcgctcat gccaactgcg
     2101 atcttcgccg cgttggtacc cacaacaccg ccgccgatga tcaccactcg agccgacggc
     2161 acaccgggaa cgccacccaa cagaattccg cgtccgccac tcatcttctc gagatagtgc
     2221 gcgccgacct gaatagccat gcgcccggcg acttccgaca tcggcgttag caacggcaac
     2281 gtgccacgtc gatcagtaat cgtctcataa gcgatacccg tgactttgcg ctcgatcaat
     2341 tgtttagtca gctcgcgatc cggagcgaga tgcagatacg tgaagagcag ttgaccttcg
     2401 cgcatgcgcg gatactccgg g
//
LOCUS       DASTEY010000268         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1291299, whole genome shotgun sequence.
ACCESSION   DASTEY010000268 DASTEY010000000
VERSION     DASTEY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291299"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFO72_04735"
     CDS             complement(<1..442)
                     /locus_tag="VFO72_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9478623.1"
                     /translation="MRVLVTGGAGYIGSVVSEQLVNDGHEVVIYDNLSKGHRGAVVEG
                     ARFVQGDLLEADQLRQTLNDNRIEAVIHMAASSLVGESVQEPSKYYHNNVVAGLVLLN
                     AMRECQIKRIVFSSTAATYGEPEEQPIRETTPTNPTNTYGETKLA"
     gene            complement(482..1243)
                     /locus_tag="VFO72_04740"
     CDS             complement(482..1243)
                     /locus_tag="VFO72_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET9478624.1"
                     /translation="MKAELQQLVALQTLDTRIRTLQKELEAIPERRAEIEKDFDQRAF
                     EIRALESRRDEAKHTRARLENEVVEQRGRAERAERNLMSSKKQEEYTAAIREADSARK
                     QISALETQILEQLEQLEQAEAALKERADEIASLNSDREARLKAFDEESETIGERLANA
                     RKERDEVFANLPKQMSGLYTRIKTRIRDGVAVAEARNRSCSACFMSLRPQVMAEIRRG
                     EEVITCDNCGRILYYVPTDSLQADAAAPQPNVAVT"
     gene            complement(1429..1830)
                     /locus_tag="VFO72_04745"
     CDS             complement(1429..1830)
                     /locus_tag="VFO72_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9478625.1"
                     /translation="MLVTAVTDDGPAAKAGVRAGDVITAVDGEAIDSPGDVSRAINRK
                     KEGDVTLTVVRNKSQQTIRVTPSEGGGFPGTLGRPQVGRSIVIPRIEIPTPEIDIVMP
                     SIVIPSIPSVNVNLPRIRVTPRVIRSERGPI"
     gene            complement(1855..>2088)
                     /locus_tag="VFO72_04750"
     CDS             complement(1855..>2088)
                     /locus_tag="VFO72_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478626.1"
                     /translation="EVTATIGKRSNSAFTSGDLLSGPRRAWKWEGMEPREWKFEGPLK
                     ERLFEHNGDLTFMLSNSRRIGVSTTELTKQLAD"
BASE COUNT          413 a          634 c          529 g          512 t
ORIGIN      
        1 acgcgagctt tgtttcgccg taagtattcg taggattggt aggagtagtt tcccggatcg
       61 gctgctcttc gggttcgccg tacgttgctg cagttgaaga aaaaacgatg cgctttatct
      121 gacactcgcg catcgcattg agcaacacca gccccgcgac aacgttgttg tggtaatact
      181 tcgacggctc ctgaaccgac tcacccacga gtgaggacgc agccatgtga atcacagctt
      241 cgatgcggtt gtcgttgagt gtttggcgaa gctggtccgc ttcgaggaga tccccttgaa
      301 cgaaacgcgc gccttcaacc accgccccac gatgaccttt cgagagattg tcgtaaatca
      361 caacctcgtg gccatcgttc accaactgct cactaaccac acttccaata tagccggcac
      421 ctccggttac aaggacgcgc atgcaaacac cccccttgga aactaaaagc aatttaaact
      481 gctaagtaac ggcaacgttc ggctgaggcg ctgccgcgtc tgcctgtagt gaatcggtcg
      541 gtacgtagta gagtatgcga ccacagttat cgcaagtgat cacttcttcg ccacgacgaa
      601 tctcggccat cacctgcggc cgcaacgaca tgaagcacgc cgaacacgag cgattacgcg
      661 cttccgcaac ggcgacgcca tcgcgaatac gagtcttgat tctggtataa agcccgctca
      721 tctgtttcgg taaattcgca aacacctcgt cgcgctcttt gcgcgcattg gcgagacgct
      781 cgccaatcgt ttcgctctct tcgtcaaacg ccttcagtcg cgcttcgcga tccgaattca
      841 ggctcgcgat ctcgtctgct cgttccttca aagcggcttc tgcctgttcg agttgttcga
      901 gttgttcgag tatctgcgtc tcgagggcag agatctgctt gcgagcggag tctgcctcgc
      961 gaattgcggc cgtgtattcc tcctgttttt ttgaagacat caggtttcgt tctgcccgtt
     1021 cggctcggcc gcgctgttcg accacctcgt tttcgagacg cgcgcgagtg tgttttgcct
     1081 catcgcgacg gctttccagg gccctaattt caaaggcgcg ctgatcgaag tctttctcga
     1141 tctctgcgcg cctttcgggt atcgcttcca gttctttttg taatgttcgt attctggtat
     1201 ccagggtctg caaagcgact aactgctgca attccgcttt cactctatgc actcctcaaa
     1261 agatcagggc tgtaaatgac ggctcccatg gctctcagga tcatcacaca acccgagcgt
     1321 ccggcgcaag cgctggatat aagccgcaga tgaacgcgga tttcatgcac tgatttattt
     1381 aaatcagtgc atgaaatccg tgttcatctg cggcttatat ttccttaact agatcggccc
     1441 gcgctcggat ctgatcactc gcggcgtcac gcggatgcgc ggcagattga cattcaccga
     1501 cggaatcgat ggaatcacga tgctcggcat cacgatgtcg atttcgggag tcggaatctc
     1561 gatccgcggg atcacgatgc tgcgtccaac ctgcggccgt cctaacgtcc cgggaaagcc
     1621 accaccctcg ctcggcgtca cgcgaatcgt ttgctgcgac ttgtttcgaa ccaccgtcag
     1681 cgtcacgtcc ccttctttct tacgattgat ggcgcgcgag acgtcgcccg gagagtcgat
     1741 cgcttcgccg tcaacagccg taatcacgtc accagctcgc acaccggcct tcgccgctgg
     1801 tccatcgtca gtcaccgcag tcaccagcac gcctttgccg cccgtgacac caaactagtc
     1861 agccagctgc ttggtcaatt ccgtggtact cacgccgatt ctgcgactgt tgctgagcat
     1921 gaacgtgagg tcgccgttgt gctcgaaaag acgctctttt agtgggcctt caaatttcca
     1981 ctcccttggc tccatgcctt cccacttcca ggcgcgccgc gggccgctga gaagatcccc
     2041 actcgtaaat gcggagttgc tgcgtttgcc gatcgtggca gtgacttc
//
LOCUS       DASTEY010000269         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1423275, whole genome shotgun sequence.
ACCESSION   DASTEY010000269 DASTEY010000000
VERSION     DASTEY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423275"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFO72_04755"
     CDS             complement(<1..563)
                     /locus_tag="VFO72_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HET9478627.1"
                     /translation="MDLSITVNGMKFPNPFVLGSGPPGTNARVLAKSYDAGWGGAVAK
                     TVSLESAKVVNVVPRYGKLRSRRTGEVIGFENIELISDRPIDVWLDEFRQIKKEYPHN
                     ILIASIMEEYEKSRWQELTRMVQETGVDGFELNFSCPHGMTERKMGSEMGEHPDLTEE
                     VTSWVTEVATIPVWAKMTPNITNIREPA"
     gene            complement(664..1533)
                     /locus_tag="VFO72_04760"
     CDS             complement(664..1533)
                     /locus_tag="VFO72_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET9478628.1"
                     /translation="MPRTVKCGLIQCTNAAPTDLPIEEIKRLNIEKHLTFLEEAAKQG
                     VQIVCMQEVFTTPYFCAEQQTRWYEAVERIPDGQTVRLMQEVAKENGMVIIVPIYEEE
                     ITGVYYNTAAVIDADGKYLGKYRKNHIPHVNPGFWEKFYFRPGNLGYPCFDTAFARIG
                     VYICYDRHFPEGARALGLNGAEIVFNPSATVAGLSEYLWRLEQPAHAVANGYFIGAIN
                     RVGHEQPWDIGEFYGQSYFCDPRGQFIAEAPRDRDALVVADLDLDKIREVRNTWQFFR
                     DRRPDTYGALVAD"
     gene            complement(1533..>2728)
                     /locus_tag="VFO72_04765"
     CDS             complement(1533..>2728)
                     /locus_tag="VFO72_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HET9478629.1"
                     /translation="SFPVLSRAAFGTRGANVPAMLRAIVACGWFGIQTWIGGTALDAL
                     FGFMFTAWNSLSSTFLDVALHTWIAFFLFWAIQVFVIMKGVEGIKYLETWSAPLLLAG
                     GLVLLVWASWRAGGLGRVLTESTALQKERGNFWQIFPGALTASVGYWATLSLNIPDFT
                     RYARSQKSQMLGQALGLPLTMTGFAFIGVAVTSATLLIYGVAIPNPVDLMARFDNVLI
                     ILFATAVIFAAQLTTNMAANVVSPSNDFSNLNPRLISYVTGGLITAVIGILMMPWKLM
                     ASMGAYIFTWLIGYSGLMGAIAGILICDYWVIRNQHLDLEGLFDPEGPYSYSRGFNWR
                     AIIALTAAVAPVVPGFVRAAVTPGGQVGDPTLFDQLYTYAWFVTFALGFVIYYVLTAS
                     RRESF"
BASE COUNT          640 a          770 c          735 g          583 t
ORIGIN      
        1 gcaggctcgc gaatgttggt gatgtttggc gtcatctttg cccacactgg aatcgtcgca
       61 acctcagtca cccaactggt cacttcctca gtcagatccg gatgctcgcc catttccgaa
      121 cccatctttc gttcggtcat gccgtgcggg caggagaagt tgagttcaaa cccgtcaaca
      181 cccgtttcct ggaccatccg cgtgagctcc tgccaacggc tcttctcgta ctcctccatg
      241 atggaagcga tcaggatgtt gtgtggatac tcttttttga tctggcgaaa ctcatcgagc
      301 catacgtcga ttgggcggtc ggagatcagt tcgatgtttt caaacccgat tacttcaccc
      361 gttctgcgcg aacgaagttt tccgtaacgc gggacaacgt tcactacttt ggcggattcg
      421 agactgactg tcttggcgac ggcgccaccc caacccgcgt cgtacgactt agcgagcact
      481 cgggcgttgg ttcccggtgg acccgaaccc agcacaaacg gattggggaa cttcatgccg
      541 ttgacagtga tggataggtc catgaaaaac tcctttgttg ccgattttcg attgccgatt
      601 gccgatttac gccacggaga ctgtcttcaa attggcgatc ggaatcgaaa atcggcaatt
      661 tctttaatcg gcgacgaggg cgccgtaagt gtccggacgt cggtcgcgga agaactgcca
      721 cgtgtttcgc acttcgcgga tcttgtctag atccaaatcc gcaacgacga gcgcatccct
      781 gtcgcgtggc gcctcggcaa taaactgccc gcgcggatcg cagaagtaac tctgcccgta
      841 aaactcgccg atgtcccacg gctgctcgtg tcccacacga ttgatcgcgc cgatgaaata
      901 accgttagcg acggcgtgcg cgggttgttc gagacgccac aaatactcac tgagtcccgc
      961 aaccgttgcc gatggattga aaacgatttc ggcgccgttc aacccgagag cgcgcgcgcc
     1021 ttcgggaaaa tggcggtcgt aacagatgta gacgccgatg cgtgcgaagg cggtgtcgaa
     1081 acaaggatag ccgagattcc cgggccggaa atagaatttt tcccagaagc cgggattgac
     1141 gtgtggaatg tggttctttc gatacttccc gagatacttc ccatcggcgt cgattaccgc
     1201 tgctgtgttg tagtagacgc ccgtgatctc ttcctcatag atcggcacga tgatcaccat
     1261 gccgttttct ttcgccactt cctgcatcag gcgcacagtt tgaccatccg ggatgcgttc
     1321 gaccgcttcg taccaacgcg tctgttgttc ggcgcaaaag taaggcgtcg taaaaacttc
     1381 ctgcatgcag acgatctgca cgccctgttt tgccgcttct tcgagaaatg tcagatgctt
     1441 ctcgatattg aggcgcttga tctcttcgat cggaagatcc gtcggcgccg cattagtaca
     1501 ttggatcagt ccacatttca ccgtacgtgg cattaaaacg actcccttcg cgatgcagtc
     1561 aaaacgtagt atatgacgaa tccaagagca aaagtaacga accatgcgta cgtgtaaagt
     1621 tgatcgaata gcgttggatc gccgacctgt ccacccggcg ttacggcggc acgtacgaac
     1681 cccggcacaa cgggagcgac tgctgctgtg agcgcgatga ttgcccgcca gttgaaaccg
     1741 cggctatacg aatatggccc ttccggatca aaaaggcctt cgaggtctag atgttggttg
     1801 cgaatgaccc agtaatcgca gatcagaatg ccggcgatcg cgcccattaa acctgaataa
     1861 ccgattaacc aggtgaagat gtaagcgccc atcgaagcca tcagtttcca cggcatcatc
     1921 agtattccga tcacggcggt gattagaccg ccggtgacgt atgaaataag ccgcggattc
     1981 aagttagaaa aatcattgga gggtgagacg acgtttgcgg ccatgttggt cgtgagctgc
     2041 gccgcaaaga tcaccgcggt tgcaaacagg atgatcagca cattgtcgaa acgcgccatc
     2101 agatccacgg gattagggat ggcaacgcca tagatcaaga gcgttgcgct ggtcactgcg
     2161 acaccgatga aggcaaaccc cgtcatcgtc agtggcagtc cgagcgcctg gcccaacatc
     2221 tgcgacttct gcgaacgggc gtaacgagtg aagtcgggaa tgttgagact gagtgtggcc
     2281 cagtaaccta cggaagctgt caaagcaccg ggaaagatct gccagaagtt accgcgctct
     2341 ttctgcagtg ccgtcgattc tgtgagcaca cggcccaagc ccccggcgcg ccacgaggcc
     2401 caaaccagca ggaccaagcc gccggcgagt agtaacggtg cagaccacgt ttcgagatac
     2461 ttgatacctt ccacgccctt catgatgacg aaaacctgga tggcccagaa caggaaaaat
     2521 gcgatccacg tgtgcagtgc gacgtcgagg aatgtactgg agagagagtt ccatgccgta
     2581 aacatgaaac caaacaacgc atcaagcgcg gtgccgccga tccacgtctg aataccgaac
     2641 cacccgcacg cgacgattgc tcgcaacatc gcgggcacgt tcgctcccct cgtcccaaac
     2701 gcagcacggc tcaggaccgg aaaagaga
//
LOCUS       DASTEY010000270         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_236350, whole genome shotgun sequence.
ACCESSION   DASTEY010000270 DASTEY010000000
VERSION     DASTEY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236350"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFO72_04770"
     CDS             complement(<1..629)
                     /locus_tag="VFO72_04770"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HET9478630.1"
                     /translation="MNWEVFRAYDIRGIYPTDIDEEAYYRIAKAYVYLFKPKTMVVGM
                     DARSSSPPLKASLIKGFLDAGVDIVDIGEITTDMLYYAVGASSDYSGGVVVSASHNPK
                     EYNGMKMVREKATAISSDTGLFDIRDVLKAGKDAEVTSDKKGTCTERDVLDDYLAHVL
                     KSIDRSRIKQFTFVGNANFGYVCRPARKLAETLGLNLIPLNFEPDGSFP"
     gene            complement(647..1681)
                     /locus_tag="VFO72_04775"
     CDS             complement(647..1681)
                     /locus_tag="VFO72_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET9478631.1"
                     /translation="MQALILAGGKGTRLRPLTVYTPKPIVPICNRPFLLYQIDTLKRA
                     GITDITLSLSYQPNKIEHLLGDGSDHGVKLKYTVEPQPMGTAGAYKFAEDLIREPTVV
                     FNGDILTDLDLKAVIREHNERNATATIVLAPVENPQSYGLVETEKDGRIRRFLEKPKA
                     DEITCNTINAGTYILEPNVLDYLPAGENYSFEYGLFPDLLKRGEAFYAHIPARTYWID
                     IGTPERYLQVHSDLLNNRVSRFHIKERRGQFDSATVAEIDEISMIGDDCQIKPGAQII
                     NSVLGQGCFIEERARVENSVIWPHTRIGTTAQLNGAIVGRGCHIGRSAVVGPGTVLGD
                     KTSLTDYTFT"
     gene            1742..>2788
                     /locus_tag="VFO72_04780"
     CDS             1742..>2788
                     /locus_tag="VFO72_04780"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET9478632.1"
                     /translation="MPELNEAVIISAARTPVGKFLGSLKGFSATELGAIVVRESVKRA
                     GVKPEDVDEVIMGCVIQAGLGQNPARQAALNGGLPSTVSAVTINKVCGSGLKAVMLAA
                     QAVQLGDAEIVVAGGMESMSNAPYLIPKAREGYRLGNGELVDSMINDGLWCAFENYHM
                     GCTGEVVAEEYSISRSQQDEFAVNSHRKAAAAIREGKFKDEIVPVEIKQKKGPALMFD
                     TDETVREDTTAEVLGKLKPAFKSEGGTVTAGNAPGVNDGASALVVTSLRRARELGVEP
                     MARIVAQATSGVEPRMVMMAPVEAVRKLFKKTGWSPSEVDLVELNEAFSVAAVAVTRE
                     LGLDPEKVNVNGGAV"
BASE COUNT          623 a          662 c          846 g          657 t
ORIGIN      
        1 tttggaaatg atccgtcggg ctcaaagttc aaagggatga gattcaaacc gagtgtttca
       61 gcgagtttcc ttgccggtct acagacgtat ccaaagttgg cgttcccgac aaaagtgaac
      121 tgtttgatgc gactgcgatc gatggatttc agaacgtgcg cgagatagtc gtcgagtacg
      181 tcgcgctcgg tgcacgtccc ttttttgtca gaagtgactt cggcatcttt gccggctttc
      241 aaaacgtcgc gaatgtcaaa caaaccggtg tcggaagaga tcgcggtggc tttctctcgc
      301 accatcttca tgccgttgta ttcttttgga ttgtgcgagg cggagacaac gacgccgccc
      361 gagtaatcag aagaggctcc aactgcgtaa taaagcatgt cggtggtgat ctctccgatg
      421 tccacaatgt caactcccgc gtcgaggaag cctttgatga gactagcctt taagggaggc
      481 gacgacgaac gcgcatccat gcccacaacc atcgtctttg gcttgaatag atagacgtaa
      541 gctttggcga ttctgtaata cgcctcttcg tcgatgtctg tgggatagat accacgaatg
      601 tcgtacgctc gaaaaacttc ccaattcacg attctgcctc cctaactcag gtgaacgtgt
      661 agtcggttaa tgacgtcttg tcgccgagta ccgttcctgg tccaactacc gccgaacgac
      721 cgatgtgaca accacgacca acgatcgcgc cgttcaactg tgccgtcgtc ccgatgcgcg
      781 tgtgcggcca gatgacgctg ttttccacgc gcgcgcgttc ctcgatgaaa cagccctggc
      841 ccaacaccga attgatgatt tgcgcgccgg gttttatctg gcagtcgtcg ccgatcatcg
      901 atatttcatc aatctccgcg actgtcgctg aatcaaactg cccgcgacgc tccttgatat
      961 gaaaccggct gacacgattg ttcagcagat cgctgtgtac ctgtagatac ctttccggcg
     1021 ttccgatatc gatccaatac gtgcgcgccg gaatatgcgc gtagaaggcc tcgccacgtt
     1081 tcaggagatc gggaaacaag ccgtactcaa aagaatagtt ctcgcccgca ggtaaataat
     1141 ccagcacgtt gggttcgaga atgtacgtgc ccgcattgat cgtgttgcac gtgatctcgt
     1201 cagcctttgg cttttcgaga aagcggcgaa tgcgcccgtc tttttcagtc tcgaccaacc
     1261 cgtaactttg tggattctca accggcgcca gtacgatcgt tgccgtggca ttgcgttcgt
     1321 tgtgctcgcg aatgacggct ttgagatcca gatcggtcag aatgtcgccg ttgaaaacaa
     1381 ctgtcggttc gcgaataaga tcttccgcaa acttgtaagc gcccgctgtg cccatcggct
     1441 gtggctcgac ggtgtacttc aatttcacac cgtgatcaga gccatcgccc aacagatgtt
     1501 cgatcttatt tggttgatac gaaagcgata aggtgatgtc ggtgatgccg gcgcgtttga
     1561 gcgtgtcgat ttgataaagg agaaacggtc ggttacagat cggaacaatc ggtttgggcg
     1621 tgtaaactgt taaagggcga aggcgcgtgc ctttgccacc cgcaagaatg agtgcctgca
     1681 tgctttatga atagcgaaat aaagtctaat atcgggccaa gtctaacata gggagtttga
     1741 aatgccggaa ttgaacgaag cagtaatcat cagcgccgcg cgcacgccag taggaaagtt
     1801 cctcggatct ttgaaaggat tctcagcgac tgagctcggc gcgatcgttg tgcgcgagag
     1861 tgtgaagcgc gcgggcgtta agcctgaaga tgttgatgaa gtgattatgg gatgtgtaat
     1921 tcaggcggga ttgggccaga atcccgcgcg ccaggcggcg ttgaatggcg gtttgccgtc
     1981 gactgtttcg gcggtgacta ttaacaaagt gtgtggatcg ggtttgaagg cggtgatgtt
     2041 ggctgcgcag gctgttcaac tcggcgacgc agagatcgta gttgcgggcg ggatggaatc
     2101 gatgagcaac gcgccgtatt tgataccgaa agcgcgcgag ggttatcgac tcggaaatgg
     2161 tgagctcgtc gattcgatga tcaatgatgg gttatggtgt gcgtttgaga actatcacat
     2221 gggctgcacg ggtgaggtgg tagcggagga gtattccatt agtcgcagtc agcaggatga
     2281 gtttgcggtc aactcgcatc gcaaagctgc tgccgcgatc agggaaggga agtttaagga
     2341 cgagatcgtg ccggttgaga ttaaacagaa aaagggaccg gcgctgatgt ttgatactga
     2401 tgagacggtg cgtgaagaca cgacggcgga agtgctcggc aagctcaagc cggctttcaa
     2461 gagtgaaggt ggaacggtga cggcgggcaa cgcgcctggt gttaacgatg gggcgtcggc
     2521 gttggtggtg acgtcactgc gtcgcgcgcg cgaactgggt gttgagccga tggcgcggat
     2581 tgttgcgcaa gccacgtcgg gcgttgagcc gcggatggtg atgatggcgc cggtcgaggc
     2641 tgtgcgtaag ttgtttaaga agactggctg gtcgccgagt gaagtcgatc tggtcgagtt
     2701 gaacgaagcg ttctcagtgg cggcggtggc ggtgacacgc gaactcggac tcgatccgga
     2761 aaaagtgaac gtgaatggtg gcgccgtg
//
LOCUS       DASTEY010000271         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_236367, whole genome shotgun sequence.
ACCESSION   DASTEY010000271 DASTEY010000000
VERSION     DASTEY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236367"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFO72_04785"
     CDS             complement(<1..548)
                     /locus_tag="VFO72_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9478633.1"
                     /translation="MAKISTSPVSRLRRDLGTLEAYAALIGILIGAGIFQVTSQAWAL
                     TGPSVILGYIVLTPAILATAFPYAAYLSTSLGREPGGEYLHISRTLGGYRLAYVGAWL
                     KIISYIGAGAYLANAMAGYVIALSGGRIDAQRFHLPLALAALVVFYLIHVIGIRWFGR
                     VQVTLSALKCLALLVLILPGVFV"
     gene            complement(521..1732)
                     /locus_tag="VFO72_04790"
     CDS             complement(521..1732)
                     /locus_tag="VFO72_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9478634.1"
                     /translation="MVTIKSFDGGEFDGYLALPASGYGPGIVVIQEVFGVNDYMRSVA
                     DWYAAHGFVALCPDLFWRLERGVQLTDKGEDWNKAIDFYERIDEDKAVEDSAAALNFL
                     RQHAACSGRVGAVGFCMGGNLAYLLSARFKPDCAVGYYGVSIEKSLDEAKNLASPLLL
                     HIAERDKFCPPEAQKQIHTVLGGNRLVTLHVYADRDHAFARVGGEHYHAADAELANLR
                     SLEFFVTHLAGAGLASAQQTLSARWDDHVKYEFATRDTDDTLETMVPDAYVNHVPVMT
                     GGVGHEELREFYSQHFIPQMPPDTSMTPVSRTIGVDRIVDEMVFEFTHTSKMDWMLPG
                     VEPTGKHVRVALVVIVHFRDGKLAHEHIYWDQASVLVQLGLLDPQRLPVAGVESAEKV
                     LNPWLKSRPLP"
     gene            complement(1754..>2263)
                     /locus_tag="VFO72_04795"
     CDS             complement(1754..>2263)
                     /locus_tag="VFO72_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478635.1"
                     /translation="RSADDTVQIEIIDFKTNRIRPTKTVEPAASPYPGPKAPRLPSST
                     KETQFSFNFEETPSDASMNAALRTTSEDYELQMQSYVLAVRELVPSLSDANIRVTLHF
                     LDPNIEVHLAGELLDPARCAAAVDEAMLRIVSSADPVDFPVKTAAHCRACNFLRLCAA
                     GREFLSSSA"
BASE COUNT          499 a          627 c          694 g          443 t
ORIGIN      
        1 acaaacacgc ccggcaggat caaaaccagc aatgcaaggc atttcagtgc cgacaaggtt
       61 acctgcacgc ggccgaacca gcgaatgccg atcacgtgaa tcaggtaaaa cacgacgagc
      121 gcagccaacg cgagcggaag gtgaaacctc tgcgcgtcga tgcggccgcc actcaaagcg
      181 atgacatatc ctgccatcgc attcgcgaga taggcgccgg cgccgatgta cgaaatgatc
      241 ttgagccacg cgccgacgta agcgagacga tagccgccga gtgttcgcga aatgtgcaag
      301 tactcgccgc ccggttcgcg cccgagtgaa gtcgacagat acgcggcata gggaaacgcg
      361 gtcgctagaa ttgccggagt taaaacgatg taaccgagaa tgacgctggg gccggtaagc
      421 gcccatgcct ggctggtgac ctggaagatg cctgcgccga tgagaatgcc aatcagtgcg
      481 gcgtaggctt ccagcgtgcc aagatcccgg cgcagacgac tcacgggaga ggtcgagatt
      541 ttagccacgg attcaatact ttctccgcgc tctccacacc agcgacggga agtctctgcg
      601 gatccagcaa gccgagttgg accaacaccg atgcctggtc ccaatagatg tgttcgtgag
      661 caagtttgcc atcacggaaa tgaacgatca ccacgagcgc aacgcgcacg tgtttgccgg
      721 tcggctcgac gcccggaagc atccaatcca tcttgctcgt gtgcgtgaac tcaaacacca
      781 tctcgtcgac gatgcgatcg acgccgatcg tgcgtgagac gggtgtcatg ctcgtgtcgg
      841 gcggcatttg cggaataaaa tgctgtgaat agaactcgcg caattcctca tggcccacgc
      901 cgccggtcat cacgggtacg tgattcacgt aggcatcggg aaccattgtc tcgagcgtgt
      961 cgtcggtgtc gcgcgtggca aattcgtatt tgacgtgatc gtcccagcgg gcggagagtg
     1021 tttgctgggc gcttgcgagg cccgcgccgg cgagatgcgt gacgaagaat tcaagactgc
     1081 gcaggttcgc gagttcagcg tccgcggcat ggtagtgttc gccgcccacg cgagcgaacg
     1141 catggtctct atccgcgtaa acatggagtg tcaccaaccg gtttccgccg agaacggtat
     1201 gaatttgttt ctgtgcttct ggcggacaga acttgtcgcg ctcagcgatg tgcaacagca
     1261 acggactggc gaggttcttc gcttcgtcga ggctcttttc aatgctgacg ccgtaatacc
     1321 cgacggcgca gtcgggcttg aatcttgctg atagcaggta agccaggttg ccgcccatgc
     1381 aaaaaccgac ggcgccaaca cggccgctac acgccgcgtg ttgtcgcaga aagttcagcg
     1441 cagccgctga atcttcgacg gccttgtctt catcgattct ctcgtaaaaa tcgatcgcct
     1501 tgttccagtc ctcgcctttg tcagtcaact ggacgccgcg ttcgagccgc cagaagagat
     1561 ccggacagag cgcaacgaaa ccgtgcgccg cataccaatc agcaacgctg cgcatgtagt
     1621 cgttgacgcc gaagacctcc tgaatgacaa cgatgcctgg tccatagccg ctcgcgggca
     1681 gagcaagata gccgtcgaac tcgccgccgt cgaaagattt tattgtgacc ataagggcca
     1741 cagattacac agattatgcg gaactggaca agaattctcg gccggcggca cagagccgga
     1801 ggaagttgca ggcgcgacag tgcgccgcgg ttttcaccgg gaaatcaact ggatccgcgg
     1861 aggagacgat gcgcagcatg gcttcgtcta cggcagccgc gcaacgggct gggtccagca
     1921 gctcaccggc taagtggact tcgatgttgg gatcgagaaa gtgaagcgta acgcggatgt
     1981 tggcgtcaga aagcgacggc acgagttcgc ggaccgcgag cacgtaagat tgcatttgca
     2041 gttcgtagtc ttcggaagtg gtccgcagtg cagcgttcat cgaggcgtct gacggcgtct
     2101 cttcgaagtt aaaagagaac tgagtttctt tagtggagga gggcaggcga ggcgccttcg
     2161 gtcccgggta ggggcttgcc gccggctcaa cggttttcgt tgggcggatg cggttggttt
     2221 tgaagtcgat tatctcgatt tggacggtgt cgtctgctga gcg
//
LOCUS       DASTEY010000272         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1620839, whole genome shotgun sequence.
ACCESSION   DASTEY010000272 DASTEY010000000
VERSION     DASTEY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620839"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFO72_04800"
     CDS             <1..640
                     /locus_tag="VFO72_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET9478636.1"
                     /translation="IRSTTDLNAFREASVVIEAVVEDLATKTQVFKLLDQIAPADAIL
                     ASNTSSISITKLGAATDRPDKVIGMHFMNPVPVMKLVEVIRGMATSDETYERVRTLSE
                     KLDKIPLDCQDSPGFVSNRVLMPMINEAIFTLYEGVATRESIDGIMKLGMNHPMGPLT
                     LADFIGLDVCLAIMNVLYEGLGDPKYRPCPLLKRYVDAGWLGRKSGRGFYDY"
     gene            666..1853
                     /locus_tag="VFO72_04805"
     CDS             666..1853
                     /locus_tag="VFO72_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478637.1"
                     /translation="MAPNISHYCQKCLAANPLGQEFCSRCGTRLMIVVEPTSSRFESG
                     ASSLSTDEHLLERISAAENRTARLAERLERSLDLLLRYAQNAYFDRSLTRALISLLEE
                     DGIVQSERLERLWSERCERDALEQEEHVHRDELRLRILAHAQAMERPVFAELVNEGFL
                     LIEDKQVQEGISKLQRAAEMSDENAALNLFIGEHYFRRGKSKHARTYLTRAHEALPGD
                     VRVSLLLGLTCADDGEVEKAKTLLSEAMRQGGSSFAGHYGLGWLFVNEKNWRRALGEF
                     KQALTARPSPEAHYVLGCLYYELNRDGLAVRHLRKAVQMDESYAEAFYLLAQTYERTG
                     RKELAREALKRAGHRASTAVPLFQTTKSGFRRLMAGDKRLADVLRDDALRAFDVGTSQ
                     QSH"
BASE COUNT          446 a          557 c          671 g          476 t
ORIGIN      
        1 gattcgcagc accacggacc ttaatgcttt tagggaagcg tcggttgtga ttgaagcggt
       61 cgtcgaggat ctcgcgacca aaacgcaagt tttcaagttg ctggaccaga ttgccccggc
      121 cgatgcgatc ctcgcgtcaa acacgtcttc gatttcgatc acgaagctgg gcgcggcgac
      181 cgatcgtcct gacaaggtca ttggcatgca cttcatgaat ccggtgccgg tgatgaagct
      241 ggtggaagtc attcgcggca tggccacgtc ggatgaaact tacgaacgag ttcgcacgct
      301 gtcggagaag ttggacaaaa tcccgcttga ctgtcaggat tcgccgggat ttgtctcaaa
      361 tcgtgtgctg atgccgatga ttaacgaggc gatctttacg ctttacgaag gtgtggcgac
      421 gcgcgaaagc attgatggaa taatgaaact cggcatgaac catccgatgg gaccattgac
      481 gctggcggat ttcatcggtc tggatgtatg cctcgccatc atgaatgtgt tatacgaagg
      541 gctcggagat ccgaaataca ggccctgccc gctgctgaag cgttacgtgg acgctggttg
      601 gctcgggagg aaaagcgggc gcgggtttta cgactactga gccacggatt tcaccgattt
      661 tagaaatggc ccctaacatt tcccactact gtcaaaagtg cctggcggcg aatccgctgg
      721 gtcaggagtt ctgttcccgt tgcggcacgc ggctgatgat cgtcgtcgag cctacttctt
      781 cgcgattcga atcgggcgcc tcgagccttt caaccgacga acacctgctc gagcgtatct
      841 ccgctgctga aaaccggacc gcacgactcg cagaacgtct cgagcgcagt ctcgatctgc
      901 tcttgcgcta cgcgcaaaac gcttacttcg atcgctcgct caccagagcg ctcatctctt
      961 tgttggaaga agacggcatc gtccagtctg agcgactgga gcgtctctgg agtgaacgtt
     1021 gcgagcgtga tgctctcgaa caggaagagc acgttcatcg cgacgagttg cgcttgcgga
     1081 ttctggccca cgcacaggca atggaacggc cggtttttgc cgagcttgtc aatgaggggt
     1141 ttctgctgat cgaggacaaa caagtccagg agggaatcag caagctgcaa cgtgcagctg
     1201 agatgtcgga tgagaatgcg gcgttgaacc tgttcatcgg cgagcactac tttcgacgtg
     1261 gcaagtcaaa gcatgcacgc acgtatctaa ctcgggcgca cgaggcgttg ccgggcgatg
     1321 tgcgtgtctc gttgttgctc gggttgacct gcgccgatga tggcgaggtt gaaaaggcca
     1381 aaactctttt gagtgaggcg atgcggcagg gtgggtcttc ttttgcgggt cactacgggc
     1441 tcggctggtt gtttgtgaat gagaagaact ggcgccgcgc gctcggtgag ttcaaacagg
     1501 ctttgaccgc gcggccttcg ccggaagcgc attacgtgct cggatgtctg tattacgaat
     1561 tgaatcgtga cgggttggct gtcaggcatc tgcgcaaggc ggtgcagatg gatgagagtt
     1621 atgccgaggc gttttacctg ttggcgcaga cgtatgagcg taccgggcgc aaggagttgg
     1681 cgcgcgaggc gttgaaacgc gcaggtcacc gggcctcgac ggcggtgccg cttttccaga
     1741 cgacaaaatc cgggttccgc cgcctgatgg cgggtgacaa gcgtttggcg gacgttctgc
     1801 gcgacgatgc attgcgagcg ttcgacgtgg gcacgtcgca acagtcgcat taaactcgtc
     1861 aaatccctgc aaaacagacc ctacgcccct atgtcgttgc ggcaaagcta atggttcggc
     1921 gcgttgacag acccgcaagc gtcgctataa tggccgtttt gtgcgcatag cccccgaatc
     1981 tgctattcgt ggctgtgcaa actgtgatgc ggatcgagtt ggtaaacctg gagcatggtc
     2041 gaggcgacct cgctcacgtg taccagccgg acgaactggg caagttcgac gagcgtgtgg
     2101 cgattgtcga acctgtagcc gtcgagggaa aagtgcggct cgccggagcc
//
LOCUS       DASTEY010000273         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_351721, whole genome shotgun sequence.
ACCESSION   DASTEY010000273 DASTEY010000000
VERSION     DASTEY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351721"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..581)
                     /locus_tag="VFO72_04810"
     CDS             complement(336..581)
                     /locus_tag="VFO72_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478638.1"
                     /translation="MTARNETHEEGPIARTIEQQTAKLPSDTFLWAAIGSIGASALLR
                     AMGKKDASLFVGDWVAPLLLFGVYNKIVKLHGSDGGH"
     gene            complement(690..1409)
                     /locus_tag="VFO72_04815"
     CDS             complement(690..1409)
                     /locus_tag="VFO72_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9478639.1"
                     /translation="MKGTAAQMSGNRTGMQTSPELAEELIEGATNAVPSSEGGPENIA
                     ECRGEYIAEGFPIGSMPALPMSEEGEADEEEIGLAVLLDKLSERLAFERMGTRLYEAL
                     LNKCEILGETSPGPTLEQIQEIGREELEHFLLLNDTIAGLGADPTVQSPGADIAAVSS
                     MGIMQVLTDPRSSIPQCLQALLTAELTDNAGWELLIELSDNLGYEEMKTEFESALAAE
                     ERHLQNVRTWLSECVLDEAQV"
     gene            complement(1406..2605)
                     /locus_tag="VFO72_04820"
     CDS             complement(1406..2605)
                     /locus_tag="VFO72_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478640.1"
                     /translation="MTLDILKEKGTPLDKQQFTWRELVQTPTSKLDDDAFTRVRIILM
                     NGLESEALRFSHATARMLNGDFRLLLAQVRRVEQHQQTMVNWLLPADLSPLETTIGFE
                     QVAIEVTAAVAQNEPDDYLAQVYRFGLLEDFDHLYRFSALMDRVMGADANNIIQSYSD
                     IMPGRPTMVEHRAPEDNLRNHYIATDAQPLTKLHALTITAAENQTHDYYMTIGPMFAD
                     PVARQLYAEIASIEEQHTTQYESLLDPNETPLEKWLLHEAMEVYNYFGCMQYETNPRI
                     KQIWERFVDYELGHLHMVMDLMKKIENRDPAELLPQTLPEPIDFESHRQFVRETLSNE
                     VDLRAAGTQFIHKDEEPDRAPLSIAYREQMNSEGSPSEIVAAGYTWTPGTELVEKKSA
                     FAKGASA"
BASE COUNT          544 a          781 c          813 g          762 t
ORIGIN      
        1 gggcatgaac gggcaccgtg aactcgtaca ctgagggaca ataaacctgt gaccgcaggt
       61 gtctggcccg cgtactgtcc taatctgaac gccagaggcg ttcgctaatt ccagcccagg
      121 gttgcttcaa ccctgggtcg aacgtaatca gtccccaggg ttgtcgcaag ctccaacctc
      181 tgggctggaa ttcgataacg ccttcggcgt tcagattagg acaccaccct cgcccgcaag
      241 gtgtcgcata ggaagcttgc gtgagttgca ggcgagacgc ctgcggtccc aggtattccg
      301 ttttttagtg ctgccggttt agttcagagt ttgtgttagt ggcctccatc tgatccgtga
      361 agcttcacga tcttgttgta aacaccaaac agcagtagcg gcgcaaccca atcgccgaca
      421 aaaagactcg cgtctttctt tcccatggca cgcagcagtg ctgaagctcc gatggagcca
      481 atcgctgccc agagaaacgt gtcagaagga agttttgccg tttgctgttc gatcgtgcgt
      541 gcgatcggtc cttcctcgtg tgtctcgttt cgcgcggtca tttgaccttg cccgcttctc
      601 atttggcctt cgccagttgt cctttggctt tgaccgaaac cggtctgacc cattgttgta
      661 ctcatttgta tcctcccctt gttgaattcc taaacttgtg cctcgtccaa aacgcactct
      721 gatagccatg tgcgaacgtt ctgaagatgt cgttcttccg ctgcgagagc cgactcaaac
      781 tctgtcttca tctcctcgta gccgagattg tcagacagct cgatcagcaa ctcccaaccg
      841 gcgttgtccg tcagttcggc ggtcaacagt gcttgcaagc actgcggaat cgaacttctc
      901 ggatcggtga gcacttgcat gatgcccatc gaggagaccg cggcgatgtc cgcacccgga
      961 ctctgcaccg tcggatctgc gcccaagccg gcgatcgtgt cgttgaggag caagaagtgt
     1021 tccaactctt cgcgcccgat ctcctggatc tgttcgagtg ttggacccgg actggtttcg
     1081 ccgaggatct cgcatttgtt cagcaacgct tcgtacaaac gggttcccat acgctcgaac
     1141 gcgagccgct cgctcaattt gtccaacaac actgcgaggc caatctcctc ttcgtccgcc
     1201 tcgccttcct cggacatggg cagcgccggc atcgatccga tcggaaatcc ttcggcgatg
     1261 tactcgccgc ggcattcagc aatgttttcc ggaccaccct cagaactcgg aactgcatta
     1321 gttgctcctt cgatcaactc ttcagcgagc tcgggcgacg tttgcatgcc cgtgcgatta
     1381 ccactcattt gtgctgctgt gcctttcatg ctgatgctcc tttggcaaat gcgctcttct
     1441 tttcgactag ttcggttccg ggcgtccacg tgtatccagc cgcaacgatc tctgacggcg
     1501 aaccttcaga gttcatctgt tcgcgataag cgatcgacag cggcgcgcga tccggctcct
     1561 cgtctttgtg aatgaactgc gttcccgctg ctcgcaggtc aacttcgttg ctcaacgtct
     1621 cgcgcacaaa ctggcgatgg ctttcgaaat cgattggctc gggcagcgtt tgcggcagca
     1681 gttcggcggg atcgcgattc tcaatcttct tcatcagatc catcaccatg tgcaggtgtc
     1741 cgagttcgta atcaacgaat cgttcccaga tctgcttgat gcgcggatta gtttcgtact
     1801 gcatgcagcc gaagtagttg tagacctcca tcgcctcgtg taacagccat ttctcgagcg
     1861 gcgtttcgtt aggatcgagt agcgactcgt attgcgtagt gtgttgctcc tcgattgacg
     1921 caatctcggc gtagagctgg cgcgcgactg gatcggcaaa catcgggccg atcgtcatgt
     1981 agtaatcgtg tgtctggttt tcggccgccg tgatcgtgag cgcgtggagt ttcgtcagcg
     2041 gctgtgcgtc agttgcgatg tagtggttgc gcagattgtc ttccggcgct ctgtgttcga
     2101 ccattgtcgg ccgtcccggc atgatgtcgg aataactctg aatgatgttg ttggcgtcgg
     2161 cgcccataac gcgatccatt aaagcggaga agcggtatag atgatcgaag tcctcgagga
     2221 gtccgaagcg atatacctgt gcgagatagt cgtctggttc gttctgcgcg acggcggccg
     2281 tcacttcgat tgcgacttgt tcgaagccga ttgtggtttc gagtggtgat agatcggccg
     2341 gcagcagcca attgaccatc gtttgctggt gttgttccac gcgtcgtact tgcgcgagta
     2401 acaggcgaaa atcgccgttc aacatgcgtg cggttgcgtg cgagaagcgc agtgcttcgg
     2461 attcgagtcc gttcatgaga atgattcgca cgcgcgtgaa tgcgtcgtcg tcgagtttgc
     2521 tggttggcgt ttggacgagt tcgcgccagg tgaattgttg tttgtcgagg ggtgtacctt
     2581 tttctttaag tatgtcgaga gtcatgcatc tccttaccac gcgcgacacg aagacacctc
     2641 gacgcatcgc acgcgctctt gtttggttat atgtgtgttg gggacagtag gagaagtgtg
     2701 agttgagttc ggtacggtga cggacagtcg gattaattgt gttgggtcag gaaagctttc
     2761 gtgaagtttg acttccgatg gccgcgatgg gagcgaaaat ttgattcgac gtacgcgtgc
     2821 ttgcgtacac gacgattggc agtttggttc agagtgcgag gaactggtgt cagcgcagcg
     2881 atcttttcaa tttatgttga
//
LOCUS       DASTEY010000274         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_797069, whole genome shotgun sequence.
ACCESSION   DASTEY010000274 DASTEY010000000
VERSION     DASTEY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797069"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VFO72_04825"
     CDS             <1..1209
                     /locus_tag="VFO72_04825"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET9478641.1"
                     /translation="NPPIMGANIQEDYPNYHSVTMRREMPRSTCRLCGECDIGCNYGS
                     KNTLDYNYITAAIHQRPYPATVETLCEVKVIAPLAGAGYQVEFVRHNPAAIDPLTGKC
                     PPPPRFTITCKRLILAAGTFGSPYLLLRNRSAFPHLSPVTLGSRFSVNGDLLSFIQNS
                     RERRNGQMVPRRLDPSFGPVITSAIRVGDTLDGDGPQGRGFYTEDGGNPYLLSWFAEL
                     QGLPSTLKRALKFGKMRLKYRWGLNNDANLSAEIADLLGPANASMSSFPVLTMGRDFA
                     NGRLSLKGDLLECDWTQQKSQAYFDRVRRVGRSIAAALNAEYRDNPSYTYLHQILTAH
                     PLGGCPMGISREDGLVDSFGETFDYPGLYVVDGSVLPGPVGPNPSLTIAAIADRAADH
                     IIQQHTQEHA"
     gene            1247..2809
                     /locus_tag="VFO72_04830"
     CDS             1247..2809
                     /locus_tag="VFO72_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9478642.1"
                     /translation="MIDGNKKRALILAGGGIKVSFQAGVLQVWLDEAGLTFDHVDAAS
                     GGVFNLTMMCQGMSGLQIADNWRNLNPRGGSSFNPASFLNLLYAESLFTLDNYRKHVF
                     TSWGLDWNKIRASKLNATFNVVNFSTKTLEVVEPKDMTEDLLCACVSLPMWFPPVRIN
                     NQTYIDAVYLTDANIDEAIRRGADEIWVIWTVSDKDEWHSGFVATYFQIIETTAVGHY
                     KAALQRIADNNAAIAAGKDGEYGRHIEVKELKADVALHYLIDFSQDRLAETVNLGVWR
                     ARQWCKENGMALKPHTGEFPTDVHEAETKVSFTEEMKGYVTFGELDYERGAKLGKESG
                     TYIMFHLTITINGVNRFVTDPDHDTEDVVGYIKCDALGGQRPVEKAKFNLFIDDHDPS
                     IKRMLYRLFFSDAQGNPLTLSGFKYIKDDPGLDLWHDTTTLFTRILNGHVSAEQEAAA
                     APDPEKLKQLVKASGIIIIYFFDFMKQLTTFRAEGPSLADRAAAMARFGRLFMGKLWD
                     VYARNVLTSGPF"
     gene            complement(2806..3759)
                     /locus_tag="VFO72_04835"
     CDS             complement(2806..3759)
                     /locus_tag="VFO72_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9478643.1"
                     /translation="MTVAFVVTVAIFLGLLIFFANEVRRPVRDSKWDHRPLWRKRFRL
                     ALITILLLGVGATGWAFFIEPNRLVTRHQTLHIDQWPSGLSDLKIAVLADIHAGGWAI
                     DDRKLRLIVERTNQLQPDLIVIPGDFVHGDGRRDPDTVAPEVFAPALKDFRAPLGVYA
                     VLGNHDWWYDGQRVRRALEANGIKVLENEVAKIEARGTSFWLAGLADLWTRPQYVHET
                     IAGVPAGETVIAFTHNPDIFPLLPQRVPLLIAGHTHGGQVRLPLIGSVAHVSKFDDRY
                     EVGHVIENGHHLFVTTGIGTSIVPVRFGVPPEIVLVTLKSA"
     gene            complement(3794..4504)
                     /locus_tag="VFO72_04840"
     CDS             complement(3794..4504)
                     /locus_tag="VFO72_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478644.1"
                     /translation="MRYVAALTLALLAVMLLPRTHFSQGGQTETSRGVAGGGISVAGW
                     TGKIDAKEEAAGLTLNHAKLAKEGDALHVVTGPAVTYWNPENKASGDYTVKATFKEPK
                     YMNLNNHPHPYGITIAGNDLGTDQQSYLYCAAYGNGKFIIRGFGPAPFQMNGPRGAED
                     AAVNKAAGVGEPVTQEIAMTVKGDKVECAINNKVVASYDKSALVTAGKLKSTDGIYGL
                     RFAHNTEALVSGLTVSKP"
BASE COUNT         1030 a         1273 c         1274 g         1003 t
ORIGIN      
        1 aatccgccga tcatgggcgc aaatattcag gaggactatc cgaactatca ctcagtcacg
       61 atgagacgag agatgccgcg ctcaacctgc cgtctctgtg gcgagtgtga catcggatgt
      121 aactacggca gcaagaacac gctggattac aattacatca ctgccgccat ccaccagcgg
      181 ccgtatccgg caacggtcga gacgctctgc gaagttaaag ttatcgctcc tcttgcgggc
      241 gctggttatc aggtcgagtt tgtccggcac aatcccgcgg caatcgatcc gttaaccgga
      301 aagtgtccac ccccaccgcg attcaccatc acgtgcaagc gtttgattct cgctgcggga
      361 acgtttggct caccttacct gctgctgagg aaccggtctg cttttccgca cctcagtccg
      421 gtgacactcg ggtcgcgttt ttccgtcaac ggagatctgc tgtcattcat ccagaacagc
      481 cgcgaacgtc ggaacgggca gatggtgcca cgacgtttgg atccgagttt tggaccagtg
      541 atcacgagcg cgattcgagt cggcgacaca ttggacggcg atggaccaca gggccgcggc
      601 ttttatacgg aggacggcgg caatccgtat ctgttgagct ggttcgcaga actccagggc
      661 ctgccgagta ctcttaaacg cgcactcaag ttcggcaaga tgcggttgaa gtatcgctgg
      721 ggcttgaaca acgatgcgaa tctgagcgcc gagattgctg atctactcgg tccggcaaac
      781 gcctcgatga gttcatttcc tgtcctcacg atgggccgcg acttcgccaa tggccggctg
      841 tctctgaaag gtgatctgct cgagtgtgat tggacgcaac agaagtcgca ggcgtacttc
      901 gatcgtgtgc gacgtgtcgg tcgaagcatc gccgccgctt tgaacgccga atatcgcgac
      961 aatccttcgt acacatactt acaccagata ctcactgcgc atccgctggg tggctgtccg
     1021 atgggcatca gtcgtgagga tgggctggtg gattcttttg gcgagacgtt cgattatccg
     1081 ggtttgtatg ttgtcgatgg ttccgtgttg ccgggtcccg taggtccaaa cccgtcgctt
     1141 acaatcgccg cgatagcgga tcgagccgcc gaccacatca tccaacaaca cacacaagaa
     1201 cacgcttaag cagctaaaaa cgctcgtcgc ataaaggagt aaacagatga tcgacggcaa
     1261 caagaaacgc gcgctgattc tcgcgggtgg aggcatcaag gtctcgtttc aggcaggcgt
     1321 gctacaagtc tggctggacg aagcgggatt aactttcgat cacgtcgatg ctgccagcgg
     1381 cggtgtcttc aatttaacaa tgatgtgcca gggcatgagc ggtttgcaga ttgccgacaa
     1441 ctggcgaaat ctcaacccgc gcggaggcag cagtttcaac cctgcttcgt ttctaaacct
     1501 gctctacgcc gaatcgctct ttactctcga caactatcgc aaacatgtct ttaccagctg
     1561 ggggttggat tggaacaaga tccgcgccag caaactcaac gccaccttca acgtagtcaa
     1621 cttcagcact aagacactcg aggtcgtcga gccgaaagac atgaccgaag acctgttgtg
     1681 cgcgtgcgtg tctttgccga tgtggtttcc accggtacgt atcaacaatc agacttacat
     1741 cgacgccgtg tatctgacgg acgccaacat cgatgaagcg attagacgcg gtgcggacga
     1801 gatctgggtc atctggacag tgagtgacaa agacgagtgg cattcgggat ttgtcgccac
     1861 ttacttccag atcatcgaga ccaccgccgt gggccattac aaagcagcgt tgcagcgaat
     1921 cgctgataac aatgctgcaa tcgccgctgg aaaagacggc gagtacggcc ggcatatcga
     1981 ggtaaaagaa ctcaaggccg acgttgcact gcactatctg atcgacttca gtcaggatcg
     2041 tctcgcagaa acagtcaatc tcggcgtttg gcgtgcgcgc caatggtgta aggaaaatgg
     2101 aatggcgctg aaacctcata ccggagagtt tcccactgac gtccacgagg ctgagacgaa
     2161 agtcagcttc accgaggaga tgaaaggtta cgtcaccttt ggtgaactcg actacgaacg
     2221 cggcgctaaa ctcgggaaag agagcggcac ctacatcatg tttcatttga ccatcacgat
     2281 caacggggtg aatcggttcg taaccgatcc ggatcacgat acggaagatg tcgttggtta
     2341 tatcaaatgc gacgcgttgg gtggacagcg gcccgttgag aaggctaagt tcaatctttt
     2401 tatcgacgat cacgatccgt cgatcaagcg gatgttgtac cgtttgttct ttagcgacgc
     2461 tcagggaaat ccactaacgc tgagcggctt caaatacatc aaagacgatc ccggcctgga
     2521 tctctggcac gatacgacca cactcttcac gcgcattttg aacggccatg tcagcgccga
     2581 acaagaggcg gccgcagcgc cggatccaga aaagctcaaa caactcgtca aagcttcagg
     2641 aatcatcatc atttacttct tcgacttcat gaagcaactg acaacgtttc gcgcggaagg
     2701 accgtctctc gcagatcgcg cagccgcgat ggctcgtttc ggccggctct ttatgggcaa
     2761 gctctgggac gtttatgcgc gcaacgtgct tacgtcggga ccgttttaag cagatttcaa
     2821 cgttaccagc acgatttccg gcggtacgcc gaagcgcacg ggtacgatac tagtgccgat
     2881 gccggtcgtt acgaaaagat gatgtccatt ttcgatgacg tgtcccacct catagcggtc
     2941 gtcaaatttt gaaacgtggg ccacgctgcc aatcagcggt aatctgactt gtccgccgtg
     3001 cgtgtggccg gctattaaca gcggcacgcg ctgcggcaag agcggaaaaa tgtcgggatt
     3061 gtgggtgaaa gcgatcacag tttctcccgc gggcactccc gcgatcgtct cgtgcacgta
     3121 ttgtgggcgc gtccagagat cggcaagccc agccagccaa aacgaagtgc cgcgcgcttc
     3181 gattttggcg acctcgttct ccaaaacttt aattccgttt gcttcgagcg cgcggcgcac
     3241 tcgctggccg tcgtaccacc aatcgtgatt gcccagcact gcatacacgc cgagtggcgc
     3301 gcgaaagtcc ttcaaagccg gcgcgaacac ttccggcgca acagtatcag gatctcgtct
     3361 gccgtccccg tgaacgaagt cgccgggaat aacaatcagg tcgggctgaa gttgatttgt
     3421 gcgctcgacg atcaggcgta acttccggtc atcgatggcc catccgccgg cgtgaatatc
     3481 cgcaaggaca gcgatcttga gatccgagag tccgctgggc cattgatcga tatggagagt
     3541 ttggtgacga gtgaccaggc ggttcggttc gatgaagaac gcccaacccg tggctccgac
     3601 tccgagcagg agaatcgtga tgagcgcgag gcggaagcgt ttgcgccaaa gcgggcggtg
     3661 gtcccactta gagtctcgaa ccgggcggcg tacttcgttc gcaaaaaaga tcagcagccc
     3721 gagaaagatc gcgacggtta cgacgaacgc tacggtcatc gttgatgttc accgaaactc
     3781 agcgattctc gcgctacggc tttgatacgg tcagtccgga tacgagcgct tcagtgttgt
     3841 gggcgaagcg caggccgtag atgccgtcag tcgatttcaa cttaccggct gtaaccaggg
     3901 ctgatttgtc gtaactcgcc actaccttgt tgttgatcgc gcattcaacc ttgtcgccct
     3961 ttacggtcat cgcgatctcc tgagtgacag gttcgcccac tccggccgct ttgtttaccg
     4021 ccgcatcctc ggcgccacgt ggtccattca tctgaaatgg ggccggacca aaaccgcgaa
     4081 tgatgaactt gccgttgccg taagccgcgc agtagagata gctctgctgg tccgtgccga
     4141 gatcgttccc tgcaatcgtg atcccataag gatggggatg gttgttgagg ttcatgtatt
     4201 tcggctcttt gaatgtcgct ttaacggtgt agtcgccgct ggccttgttc tccggattcc
     4261 agtacgtaac ggctgggccg gttacgacgt gcagcgcatc gccttctttg gcgagtttcg
     4321 cgtgattcaa agtcaacccg gcggcctctt ctttggcgtc gatctttccg gtccaaccgg
     4381 ccacagagat tccgccgccg gccacaccac gcgaagtttc cgtctgcccg ccctgagaga
     4441 aatgtgtgcg tggcaacaac atgacagcga gtagagcgag agtcaaagca gcaacatatc
     4501 gcatgttcag caatcctcca gatcagtgtc cacttctgcg acgcagcctg cggcgcatga
     4561 agataccggc aaacaacgcg
//
LOCUS       DASTEY010000275         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_121149, whole genome shotgun sequence.
ACCESSION   DASTEY010000275 DASTEY010000000
VERSION     DASTEY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121149"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1184)
                     /locus_tag="VFO72_04845"
     CDS             complement(141..1184)
                     /locus_tag="VFO72_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9478645.1"
                     /translation="MLFRSSPGRVLFLILLLRLTAFAQAPETVLATVNGTGITLKQVD
                     ETVLSAINPLQQQLYAIRKVALDNLIITKLLETEARAQGVSVEELRQRLTRGEISGEI
                     KVTRAQVEEAYAQNASFFASMSPDEARERLRLDLENQSRMKHYRAGVEALKKKWPVVV
                     TLPSAPVGSELDFGMSPVKGAQKAAVTIVEFSDFECPFCAQVQETLRQVMERYGSEVR
                     LVFKHLPSEGHRNSLMAARAAYCAGEQDRFWQFHDALFASRNLSREFLNETAVTLGLG
                     RETFAGCLASERSRTAVVKDIEMARRYRIDGTPSFLINGKLVRGALTFAEFSNLIERE
                     LQQFVTRKTASTN"
     gene            complement(1286..1621)
                     /locus_tag="VFO72_04850"
     CDS             complement(1286..1621)
                     /locus_tag="VFO72_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9478646.1"
                     /translation="MDRRVELVIAQIEADVSREWETAQLAELVNLSPSRFRHLFKEET
                     GETLTQYLRLRRLERAEYLLRTTFLSIKEVKKAAGLSAMSHFVQYFKQRYGVTPSAYR
                     KRLAIFVKH"
     gene            1979..>2571
                     /locus_tag="VFO72_04855"
     CDS             1979..>2571
                     /locus_tag="VFO72_04855"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HET9478647.1"
                     /translation="MGDDLRTERELSLDFLRVCEAAAIAAARTMGQGDRKYSDQVAVE
                     AMREVMDTVPMRGRVVIGEGERDEAPMLYIGEEVGGGFGVGEEIAESCPEIDIAVDPL
                     EGTNLCALGANNAIAVLAAAERGGLLNAPDIYMDKIVVGPSSRGVVDIDAPVKDNLKN
                     IARRLGRDIEDLTVIVLDRPRHKKLISDVRTAGARIRL"
BASE COUNT          580 a          731 c          719 g          541 t
ORIGIN      
        1 atctccagct tgtgataagt ctctcgaatc aacttctctc gcgccgcatc cgaacgcgct
       61 tgcaccgcga caggagtaac aaacacacag atcaagaaaa ggatagacgt aagacgatat
      121 tgtcgtgtca tgaggatctc ctagttagtt gaggccgttt tgcgagtcac aaactgctgt
      181 aactcacgtt caatcaggtt cgaaaattcc gcgaaggtga gggcgcccct gaccagcttg
      241 ccgttgatta agaagcttgg tgtaccgtcg atgcgatacc gccgcgccat ttcaatgtct
      301 tttacgaccg cggtccgtga tcgctccgac gccaaacatc cggcaaacgt ctcgcggccg
      361 agcccaagtg tcacagcagt ctcattcaaa aactcccgcg acaagtttcg tgaggcaaat
      421 agcgcgtcat gaaactgcca gaaccgatcc tgctcgcccg cacagtacgc cgcccgcgcc
      481 gccatcaaag agttacgatg tccctccgac ggcaggtgtt tgaagaccag ccgcacctcg
      541 ctgccataac gctccatgac ctgcctgagc gtctcctgca cctgggcaca aaacgggcat
      601 tcgaaatcgg aaaactcgac gatcgtgacg gcggccttct gcgctccctt caccggcgac
      661 atgccgaaat ccaactccga cccaacaggc gcagacggta gcgtcacgac cacgggccac
      721 ttctttttca acgcttcgac accggcgcgg taatgcttca tgcgcgactg gttctccaaa
      781 tcgagtcgca acctctcccg cgcttcgtcc ggactcatcg acgcgaagaa cgacgcgttt
      841 tgcgcgtacg cttcctcaac ctgcgctcgc gtgactttga tctcaccgga gatctctccg
      901 cgcgtgagcc gctggcgcaa ctcctcaacc gaaacaccct gcgcccgtgc ctcggtttcc
      961 agtagcttcg taatgatcag gttatcgagc gcgaccttcc ggatggcata gagttgctgt
     1021 tgcagtggat tgatggcact caaaacagtt tcatccacct gcttcaacgt gatccctgtg
     1081 ccgttaactg tggccaatac cgtctccggc gcctgcgcga aagcagtgag gcgcaacaag
     1141 agaatcagaa acagaactct tcctggtgag gatcggaaca acattcaatc gtctctccgt
     1201 tcgtttttat ttgtttgagg ggtggtgagc aaactgtagg tgtgatcgct gttgtgagtc
     1261 aatagaagag tgatatcgaa aatggctaat gctttacgaa aatggctaag cgcttgcggt
     1321 acgcgctcgg ggttacgccg tagcgctgtt tgaagtactg gacgaagtgg ctcatagcac
     1381 tcaaacccgc agccttcttg acctctttga tcgaaagaaa cgtcgtccgt aacagatact
     1441 ccgcgcgctc cagccgccgc aggcgtaagt attgcgtgag cgtttcgcca gtctcctctt
     1501 taaagagatg acgaaaccgc gacggcgaga gattgaccag ctccgcaagc tgcgcagtct
     1561 cccactcgcg cgagacgtct gcttcgatct gtgcgatgac cagttcgact cttcggtcca
     1621 tagtgatgag gacttgaaac gaagttgagg agggcaggcg agggtagcac gaatagatac
     1681 aaaaggcacg aaggagttcc cttgggtcgt gttcctaagt tgtgaacgcg ctctttcctg
     1741 ggaccgcagg cgtctcgcct gcactgagcg cgaagcgcga aaaacgagcg atgcagcgag
     1801 acgcctgcgg tcccaggaaa gagccttcgc aaatcaggac aagccttcgc aaatcaggac
     1861 acgacttcgc ttcgtaccgc tggtatccgg tttgcaacgt cgcgcatttt atcgcgctct
     1921 gctagtattg gcacacattc tcacgtgtct aacaaaggaa tcaggagaac cgaaacgaat
     1981 gggtgatgac ctaagaacag aacgcgagct gtctctggat ttcttgcgcg tgtgtgaagc
     2041 ggccgcaatt gcggcggcgc ggactatggg ccagggcgat cgcaagtact ccgatcaggt
     2101 ggccgtcgag gccatgcgcg aagtgatgga cactgtgccg atgcgcgggc gcgtggtgat
     2161 tggtgaaggc gaacgcgatg aagcgccgat gctttatatc ggcgaggaag tcggtggcgg
     2221 tttcggggtc ggcgaagaga tcgccgagtc gtgtccggag atcgatatcg ccgtggatcc
     2281 gctcgagggt acaaacctct gtgcgctggg cgccaacaac gccatcgccg tgctggcggc
     2341 cgcggaacgt ggtggcctgc tcaacgcacc ggatatttac atggacaaaa tcgtggttgg
     2401 tccatcgtcg cgcggcgttg tcgatatcga cgcgccggtg aaagacaacc tcaagaacat
     2461 cgcgcgcagg ctcggacgtg atatcgaaga cttgactgtg atcgtgctgg accgtccacg
     2521 tcacaaaaaa ctgatctctg acgtacgcac ggccggggct cgtattcgcc t
//
LOCUS       DASTEY010000276         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_71776, whole genome shotgun sequence.
ACCESSION   DASTEY010000276 DASTEY010000000
VERSION     DASTEY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71776"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..1783)
                     /locus_tag="VFO72_04860"
     CDS             complement(431..1783)
                     /locus_tag="VFO72_04860"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9478648.1"
                     /translation="MARKSILVVDDEKAQREILEMILADEGYDVTTAASGEAALKFAR
                     ERRFDLALTDLKMTGMDGIELLQHLLAFDSSIIVILLTAHGSIESAKEALRRGAFDYL
                     EKPYDKAALLETITRALDRLDAIDSEIVSASPKMETVKKMILKVARSNSTVLIRGESG
                     TGKELIARAVHNQSPRAAEMFQAVNCAAINENLLESELFGHEKGSFTGAHAEKKGLFE
                     IADRGTLFLDEIAELDVGIQAKLLRALQERRIRRVGGTHELGVDVRVIAATNRDLRAM
                     VSDGRFRDDLYYRINVLSIDVPPLRERREDIPVLIDYFLKKHTKNTSRLITGLTPETK
                     KLMVDYSWPGNVRQLESAIERAILLAEGDKITLEDLPTEVRQDAGPASEGTFKLPAEG
                     INFEDVERNLITQAMEQTDYNITKAAKLLGLTFRTLQYRLEKFGIKKPDGDGGESEAE
                     "
     gene            complement(1755..>2811)
                     /locus_tag="VFO72_04865"
     CDS             complement(1755..>2811)
                     /locus_tag="VFO72_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478649.1"
                     /translation="YFPVQTDLGRRFILVVLDSADILPNTFERQATRSALYTLAVLFV
                     TTIITGYFVWRFTRPIKELSIAARKVAGGNFDVQVSSERRDEMGTLASAFNEMTAKLG
                     RARELESQLHQAEKGAVVGRLAAAIAHEIRNPLNYINLTLDHLRSSFAPEDAPRRTTF
                     VRLTDQLKTEVARINRHITDFLKYSRPSKLELQDLDIRAEAEDALRLIEVRASECGIE
                     TKIVQDGTLPRVMGDRESLRSVFTNLVINAVEAIDGSGGSVSIRLSDEGQQSVKVEVI
                     DTGRGISPDDISKVFEPYFSTKETGTGLGLAIVKKAIDDHGGTISVASKEGSGTTFTI
                     ILPAREKEHGTKEHPGS"
BASE COUNT          599 a          763 c          779 g          670 t
ORIGIN      
        1 ttcgatgaac ataaacggac cacttggagc cacttgccga gagccagggc cccagagcag
       61 atcgcgttga gaaatatttc ccggatcacg ccagataacc ggtcttccgg actgcgaaaa
      121 agcggtttgt gaaacgacca gggttaagat gatcaggaga ggaatttgga gaacgggttt
      181 cgacctgcgc atgtggatga cccccttcac agaatactta caaccggaaa gagcttgtgc
      241 tgaactaatg cgtaagttgt gccattcttt aaagaagggc gtgactcgtt acttaacact
      301 tgctgtatca gatcgttagc cgaagggcag ttgaaccatc acgggcgggc gttacgtatg
      361 gacatcgtac agggcaccgg ttcagaaggc gtcacgccgg tgccagaggc agtgaagttc
      421 gttgctcgcg ctattcagct tcagattctc cgccgtcacc gtcgggtttt ttgatgccga
      481 atttctccag acgatactgc aacgtgcgaa aagtgaggcc gaggagtttt gcggctttgg
      541 tgatgttgta gtccgtctgt tccatcgcct gggtaatcag attacgttcg acgtcttcga
      601 agttgattcc ttccgcaggg agtttgaatg ttccttcaga tgctggtcca gcatcctggc
      661 gcacttctgt cggcaggtct tccagagtga tcttgtcgcc ttcggccaac agaatcgcgc
      721 gttcgatggc agactcgagt tgtcgcacgt tgccgggcca actgtagtcg accatcaact
      781 tcttagtttc cggtgtgaga ccggtgatga gccgtgacgt gttcttcgtg tgcttcttca
      841 gaaagtaatc aatgagaacc ggaatgtcct cgcgtcgttc gcgaaggggc ggcacgtcga
      901 tcgagagcac gttgatgcgg tagtagagat cgtcacggaa gcgtccgtct gaaaccatcg
      961 cgcgcagatc acgattggtg gcggcgatca cgcgtacgtc cacaccgagc tcgtgagtgc
     1021 cgcccacgcg tctgatcctc cgctcttgca acgcacgcag gagtttcgcc tggatgccga
     1081 cgtcgagttc tgcgatctcg tcgagaaaca aggtgccgcg atcggcgatc tcaaacagtc
     1141 ctttcttctc ggcgtgcgcg ccggtgaacg aacccttctc gtggccaaac agttccgact
     1201 ccagcaggtt ctcgttgatt gccgcacagt tcactgcctg aaacatttcg gctgcgcgcg
     1261 gagattgatt gtggactgcc ctggcaatta actccttacc ggttcccgac tcaccacgaa
     1321 tcaacacggt cgagtttgaa cgagccactt tcaagatcat cttcttcact gtctccatct
     1381 tgggagacgc agagacgatc tcgctgtcga tcgcatcgag acgatccaaa gcacgggtga
     1441 ttgtctcgag cagcgcagct ttgtcgtacg gtttttcgag ataatcaaag gcgccacgcc
     1501 ggagtgcttc cttagcggac tcaatcgaac catgcgcggt caggagaatg acaatgatgg
     1561 acgagtcgaa ggcgagcagg tgttgcagca gttcgatgcc gtccatgccg gtcatcttca
     1621 gatctgtcaa cgcgagatcg aaacggcgtt ccctggcaaa cttcaacgcg gcttcgcctg
     1681 acgcggctgt agtcacgtcg tagccttcat cggccagaat catttccagg atttctctct
     1741 gcgctttctc gtcgtcaact accaggatgc tctttcgtgc catgttcctt ctctctagcc
     1801 ggtaggatga tggtaaacgt agtgccactg ccttctttgg atgccacgct gatcgtgccg
     1861 ccatgatcgt caatggcctt cttcacaatc gccagaccga gacccgtgcc tgtctctttt
     1921 gttgaaaagt acggctcaaa gactttcgaa atatcgtcgg gggagatgcc gcgtcctgta
     1981 tcgatgactt ctaccttaac agactgctgg ccctcgtctg aaagcctgat cgaaacactg
     2041 cctcccgagc cgtcaatcgc ctccactgca ttgatcacga ggttggtaaa cactgagcgt
     2101 agtgattcgc ggtcgcccat cacgcgcggt agcgtaccgt cctgaacgat ctttgtttcg
     2161 atgccgcact cgctggcgcg cacttcaatt aatcgcaaag catcctctgc ctcggctctg
     2221 atatctaagt cctgcaactc gagcttggac ggtcgcgaat acttcaggaa gtcagtgata
     2281 tgacgattga tgcgcgcgac ttccgttttg agctgatcgg taagccgcac gaaggttgtt
     2341 cgcctggggg cgtcctctgg tgcgaacgac gagcgcagat gatcgagcgt caggttgatg
     2401 tagttcaacg gatttcgaat ttcatgcgcg attgccgctg ccagacgtcc gacgacggcg
     2461 cctttctcag cttgatgcag ttggctttcg agttcgcgtg cgcgtccgag cttcgcggtc
     2521 atctcgttaa acgccgaagc gagggtgccc atctcgtcgc ggcgctctga ggagacctga
     2581 acatcgaaat tcccgccggc aactttgcgc gcggcaatcg agagttcctt aatcgggcgg
     2641 gtgaatcgcc agacgaaata acccgtgatt atggtggtca caaacagcac ggccagcgtg
     2701 taaagggccg atcgagtggc ctgccgttcg aaggtattcg gcaaaatatc cgccgaatcg
     2761 aggacgacaa gtatgaaacg gcggcctaaa tcggtctgta caggaaaata g
//
LOCUS       DASTEY010000277         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_6007, whole genome shotgun sequence.
ACCESSION   DASTEY010000277 DASTEY010000000
VERSION     DASTEY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6007"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFO72_04870"
     CDS             <1..277
                     /locus_tag="VFO72_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9478650.1"
                     /translation="QPSDGAEAKELNPPDLRPLQDSELVETERRNAVQRAIGTLPEKY
                     RAPLILRDVDGRSYDEIARILETSEGTVKSRISRARGFLREKMRAYL"
     gene            289..1035
                     /locus_tag="VFO72_04875"
     CDS             289..1035
                     /locus_tag="VFO72_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478651.1"
                     /translation="MRKNNCGVVRQELDELMLDEACSTFAAEHLRECGECREFHQQQT
                     KLRQIVGSLGTVSAPADFDFRLRARLASDSSSAAYWPLMRRGFALATVLVVFATGAFL
                     VRNVMDRPETGNGVVSTNNQPAAIPEPPKQVETKKASPENSGQQLVATGAEKRPQTIK
                     NERPQSTASKTPRRLVAEDFSSKRAEVISGQEAVSGFEAISLDASLQSFKVSLDDGRG
                     NARTISVPTISFGSQRMLQTGNQFAPKRDW"
     gene            1063..>2174
                     /locus_tag="VFO72_04880"
     CDS             1063..>2174
                     /locus_tag="VFO72_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478652.1"
                     /translation="MTVRKLMQVTTFVVVLLFCAVAIAQTPAPSPKKPAPAFVNASAG
                     KVPAAPQVVTIIHRINGLKMFRLLRRSQQQVQSVASVDFAFNLMDDVHTNVIAGLAMD
                     DGETIAAWLPEAEVEFGPTDMVAPLAPATPRAPRSPRAVPAPRAGLFNGPDITVIGPE
                     GKRLAAKYVGLDAVTGLSILRLVNKNAVPAGAMKDEPLNTGQTVLLFSPEPVTKARQM
                     LRGSLYARMAAIEGRIRDVMPAPSGGVARFKVSAPKLSNANIGGVALNEAGETIGIVD
                     GLEGNEASILPAALIKRAAQRVLEQQASVPRPWLGVKGEAITALNLDQIRNHGWELQR
                     AAALAGKHHGILLTSIAPDSPAAKASLRAGDVILKV"
BASE COUNT          502 a          552 c          671 g          449 t
ORIGIN      
        1 tcaaccgagc gatggcgccg aagcaaagga gttgaatcct cctgacttgc gcccattgca
       61 ggattcggag ctcgttgaaa cggaacgacg caacgcggtg caacgtgcca ttggcacgct
      121 tccggagaag tatcgcgcgc ctctgattct gcgtgacgtt gacgggcgca gctacgacga
      181 gatcgcgcgc attctggaaa cgagcgaggg cacagtgaag tcgcgaatta gccgggcaag
      241 gggctttctg cgcgagaaga tgcgggccta tttgtaggtg aagaagttat gagaaaaaac
      301 aattgtggag tggttcgtca ggaacttgat gagttgatgc tggatgaggc gtgcagcacg
      361 tttgcggcag agcatctgcg tgagtgtggc gagtgccgtg agtttcacca gcagcaaaca
      421 aagttgcggc agatagtcgg cagtctcggc accgtgtcgg cgccggcgga ttttgatttc
      481 cgtttgcgcg cgcgactcgc tagtgattcg agtagcgcag cttactggcc gctgatgcgg
      541 cgtggatttg ccctggcaac ggtgttggtc gtctttgcta cgggcgcgtt tttagtgcga
      601 aacgttatgg atagaccgga gacaggaaac ggagtagttt ccaccaacaa tcagcccgct
      661 gctatccctg agccgccaaa acaggttgag acgaagaagg catctccgga gaactccgga
      721 cagcaactgg tagcgactgg ggcagagaag cgtccgcaaa cgatcaagaa cgaacgacct
      781 caatcaacag catcgaagac accgcggcgt ctcgtagctg aagatttcag cagcaagcgc
      841 gcagaggtga taagcggaca agaagcagtt agcggttttg aagctatctc gcttgatgcg
      901 tcgttgcagt cgttcaaagt ttcactcgac gacgggcgtg gcaatgcgcg cactatctca
      961 gttccgacga tcagttttgg ttcacaacgg atgttgcaaa ctggaaatca gtttgcgccg
     1021 aaacgagatt ggtaaacagg catcgatagg atgtatttga agatgactgt ccgaaagtta
     1081 atgcaagtca caacgtttgt ggtcgtactg cttttttgcg cggttgccat cgcgcaaact
     1141 cccgcgccat caccaaagaa acccgcacca gcgtttgtaa acgccagtgc ggggaaggta
     1201 ccggctgcgc cgcaagttgt gacgatcatc catcgcatca acggactgaa aatgtttcgc
     1261 ttgttacggc gctcccagca acaggtgcag tcagttgcct cagtggattt tgctttcaac
     1321 ttaatggacg atgttcatac caacgtcata gccggtttgg cgatggacga cggtgaaacg
     1381 attgccgcgt ggttgcccga agctgaagtc gagtttggac caacggacat ggtggcgcca
     1441 ctggcgccgg cgacaccacg tgcgccgaga tcgccgcgtg cggttcccgc tccccgtgcc
     1501 ggtcttttca acggtcctga cattacggtt atcggacctg aaggaaagcg tctcgccgcg
     1561 aagtacgtcg ggctcgatgc ggttacagga ctctcaatac ttcgcctcgt caacaaaaac
     1621 gcagttccgg cgggcgcgat gaaagatgag ccgttgaata ctggccagac agtactgctg
     1681 tttagtccag agccggtaac aaaggcgcga cagatgctgc gtgggagtct ctacgctcga
     1741 atggctgcga ttgagggccg catacgagac gtgatgcccg cgccgtcagg tggtgtcgca
     1801 cggtttaaag tgagcgcgcc caaactctca aacgcgaaca tcggtggcgt tgcgctcaac
     1861 gaggcgggcg agacgatcgg tatcgtcgat ggtctggaag gaaacgaagc gagcatcttg
     1921 cctgcagcat tgatcaaacg tgcggcgcag cgtgtattgg aacagcaggc aagcgtgccg
     1981 cgaccgtggt tgggtgttaa aggcgaggct atcactgcgc tcaatctcga tcagatccgg
     2041 aatcacggtt gggagttaca acgtgccgcc gcactggccg gaaaacatca cgggatattg
     2101 ctgacctcga ttgcgcccga cagcccggct gcaaaggcgt cgctgcgagc cggtgacgtg
     2161 atcctgaaag ttga
//
LOCUS       DASTEY010000278         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_994853, whole genome shotgun sequence.
ACCESSION   DASTEY010000278 DASTEY010000000
VERSION     DASTEY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994853"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFO72_04885"
     CDS             complement(<1..214)
                     /locus_tag="VFO72_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478653.1"
                     /translation="MENESAVNTEAGTRGRMLVADDDPAILRLIATILEKENFTVVTA
                     RDGREAYKILQADPNFTAAILDVVMPH"
     gene            complement(236..1297)
                     /gene="pdxA"
                     /locus_tag="VFO72_04890"
     CDS             complement(236..1297)
                     /gene="pdxA"
                     /locus_tag="VFO72_04890"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET9478654.1"
                     /translation="MRHSDQSSSNPGPRPRRVLPRIGITMGDPAGIGPEVVLKAVAEE
                     EIRRACIPVIIGDAQLLAHTARTLDLQSGYDIVRAEEPFPEHSEPVIYHLDNITGFIE
                     PGIESGAAGKAAGAYIEAAVELCAAGSIDAVATAPINKRALFLGGYSFPGHTEFFAHL
                     TGAEDYAMAFVAGNLRIVLLSTHVPLSEAIRLVERDRIVRRINLTNRELQRWGIEKPR
                     LAVAALNPHGAEGGLFGVEEASEIVPAIDACRRDDIDVHGPFSADTVFLRASRGEFDG
                     VIACYHDQAMIPVKCLSFGEAVNVTLGLPFIRTSVDHGTAFDIAGKGLAEHSSMVAAI
                     KLAAELSTKAGESCRPMEV"
     gene            complement(1362..1766)
                     /locus_tag="VFO72_04895"
     CDS             complement(1362..1766)
                     /locus_tag="VFO72_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478655.1"
                     /translation="MNRKLIRTTLAEVKGKAREVPQPRERSNGSSRAANTESGQARAA
                     QQQHPPVQVKKRVPPPSETNAEIFYYKKQIDAHTPMVIVLLDGEEIEGTIEWYDRGAL
                     KVNRKGAPNLLVLKRNIKYIYKADERVEKASA"
     gene            complement(2062..>2823)
                     /locus_tag="VFO72_04900"
     CDS             complement(2062..>2823)
                     /locus_tag="VFO72_04900"
                     /inference="COORDINATES: protein motif:HMM:NF023746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9478656.1"
                     /translation="SLATELDALTVAVVTKPFHFEGRRRMQQAEQGLRELRECVDTVI
                     TIPNERLLHTVDRGVSLADSFRVADDVLRQAVQGISDLITVPGLINLDFADVKSIMQG
                     MGMALMGAGRATGENRAIEATQQAISSPLLEEATIQGAKGVLINITGGPDLTLFEVNA
                     ASSIIRESADDDANIIFGAVIDEDLTDEMKITVIATGFDKESASLASTAATTVMPAAQ
                     PRYIPRPADDLPRPMMANARVDDLDVPTFIRKKAD"
BASE COUNT          624 a          796 c          766 g          637 t
ORIGIN      
        1 tatggggcat cacaacgtcc agaattgcgg cagtgaagtt tggatcggcc tgaaggatct
       61 tgtaagcctc gcgcccatcg cgcgcggtca ccacggtgaa gttctccttc tcaagaatcg
      121 tggcgataag ccgaagaata gcggggtcat cgtccgcgac gagcatacgg cctcgcgttc
      181 ctgcttcagt gtttactgcg ctctcattct ccatgtaagg ttttctccca ctaatttaca
      241 cttccatcgg acggcaactc tcgccggcct tagtcgaaag ttcggcggca agtttaatag
      301 cggccaccat gcttgaatgc tccgcgagtc cttttccagc aatgtcgaag gctgtgccgt
      361 gatccacgga agtgcggata aaaggcagac ctaaagtaac attgacggct tcgccaaacg
      421 acaaacactt caccggaatc atcgcctgat cgtgataaca ggcgattact ccgtcaaact
      481 caccgcgcga ggcgcgcaga aaaaccgtat cagccgagaa aggaccatgg acatcgatat
      541 cgtcgcggcg acacgcatcg atcgccggca caatttccga ggcttcttca acaccgaaga
      601 gtccgccttc agcaccgtgc gggttgaggg cggcaacggc aaggcgcggc ttctctatcc
      661 cccatcgctg caattcgcgg ttcgtcaaat tgatccggcg aacaatgcga tcgcgttcga
      721 cgagtctgat cgcttccgag agcggcacgt gtgtagacaa gagcacgatg cgaagattac
      781 cggcgacaaa agccatcgcg tagtcttcgg cgccggtgag atgtgcgaag aattcggtgt
      841 gtcccggaaa actgtagccg ccgaggaaaa gcgcgcgctt attaatcggc gcagtagcta
      901 ccgcatcgat gcttcctgcg gcgcacaact cgacagccgc ctcgatataa gctcccgcag
      961 ctttgcccgc cgcgcccgac tcgatcccgg gttcgataaa accggtgatg ttgtccaggt
     1021 gatagatgac gggttcggag tgttccggaa agggttcttc tgcccgaaca atgtcataac
     1081 ccgattgaag atccagagtg cgcgctgtgt gagccagtaa ctgcgcgtct ccgataatca
     1141 cgggaatgca ggcgcgtctt atttcttctt cagcgacagc tttgagcacg acctccggcc
     1201 caatcccggc tgggtctccc atggtgatgc cgatgcgcgg caaaacccgc ctggggcgag
     1261 gtcccgggtt acttgagctt tgatctgaat gtcgcattgc ttgaggcgac ggcgctactg
     1321 tccagggaca aagatgtcag tgggcgaggc tgaacaactt cttacgcgct ggctttttca
     1381 acgcgctcgt cggccttgta gatatatttg atgttccgct tgagcaccag cagattgggc
     1441 gcacccttgc gattgacctt cagcgcgcca cgatcgtacc actcgatcgt gccttcaatc
     1501 tcttcgccgt caagcagaac gatgaccatg ggagtatgag catcgatctg tttcttgtaa
     1561 tagaagatct cggcgttggt ttcgctggga ggtggaactc tcttctttac ctggactggc
     1621 gggtgttgtt gctgggcagc acgtgcctgg ccactctcag tatttgcggc gcgggaactc
     1681 ccattcgaac gctctctggg ctgcggaact tctcgagctt tgcctttcac ttccgcgagt
     1741 gtggttctga tgagtttacg attcacttga ttaatacctc caaaaatcta ggacgggtca
     1801 tttcgcccat cgcaagccat tttatgtaga cacagggatc gattaaaact ctcgcaggct
     1861 gcggccatcc gaggagagga aacgtacttt acccgtaatg gaatttcctg caaggaatgg
     1921 cgacatgcta cacaaccaga gcagaaaaag caaaggagaa aacttcttta ttttgctctt
     1981 tttactgcaa ggaatgatgc attcgtgtca tccgtgtcct cgcactgtca cagaccgaag
     2041 gacacggact acacggacgt tctaatctgc cttcttgcgt atgaaggtgg gaacgtcgag
     2101 atcgtcaaca cgcgcattcg ccatcatcgg acgcggcaga tcgtcagcgg gacgcggtat
     2161 ataacgcggt tgcgccgccg gcatcactgt ggtcgcggca gtcgatgcca acgaggccga
     2221 ctctttgtca aagccggtgg cgatgactgt gatcttcatc tcgtctgtga gatcttcgtc
     2281 gatgacggcg ccgaagatga tgttcgcatc atcgtcggca gactcacgaa tgatcgacga
     2341 tgcagcattg acttcaaaca acgtcagatc gggaccaccg gtgatattga tcaaaacacc
     2401 cttcgcacct tgaatcgtcg cttcttcgag caatggcgat gagatcgcct gttgcgtcgc
     2461 ttcgatggcg cgattctcac cggtcgcacg accagcgccc atcaaagcca ttcccattcc
     2521 ctgcatgatc gacttcacgt ccgcgaagtc gaggttgatc aatccgggca cggtgatcag
     2581 atcgctgata ccctgcacgg cctgacgcaa cacgtcatcg gcaactctga acgaatcggc
     2641 gagtgaaact ccgcggtcga cagtgtgcaa caaccgctcg ttcggaatcg tgatgacggt
     2701 gtcgacgcat tcgcgcaact cacgcaatcc ctgttcggcc tgttgcatcc gccggcgtcc
     2761 ttcgaagtgg aacggtttag taacgaccgc aaccgtcaac gcgtcgagct cggtcgcaag
     2821 cga
//
LOCUS       DASTEY010000279         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_302729, whole genome shotgun sequence.
ACCESSION   DASTEY010000279 DASTEY010000000
VERSION     DASTEY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302729"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4346)
                     /locus_tag="VFO72_04905"
     CDS             complement(<1..4346)
                     /locus_tag="VFO72_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9478657.1"
                     /translation="MLDDADVQLVLSVAALQGKLPAHRAPVVLLDSDWSLIVGHSEEN
                     PEGRITPEHLAYIVYTSGSTGTPKGVEVPHRGVVRLVTGDDYANFGPSEVFLQFAPLA
                     FDASTFEIWGSLLNGAQLVLMSSGVASLEDLGRAVTRYRVTTLWLTAGLFHQMVDEQP
                     EALAALRQLLAGGDVLSPSQVKKFVTAAPSCRLINGYGPTENTTFTCCYVMDQQVDFN
                     GSVPIGRPIANTQVFILDENLQLSPPGFVGELYAAGDGLARGYHRRASLTAERFIPHP
                     FSNEPGARLYRTGDRVRYLADGNIEFIGRADEQVKVRGYRVELGEVETALSRVPRVRQ
                     VAVVALDYGGGDKRLVAYVEAADRVSVEALQKTLRSTLPDYMMPSAFVILDELPLTPN
                     GKVDRRALPAPDEIHAASESAHTAPRTPVEELLAGIWSDVLKVERVSVSDNFFELGGH
                     SLLVTRLVSRAQAHFGVELPLKLFFESATLAEQAAQVEATLGTENGLHAPPIVPVPRN
                     GNLPLSFSQQRLWFIYQLEPSTSAYNIPTAVRLSGPLDITALSRTLNEVVRRHEILRT
                     YYRMIDGQPAQFVGPPPQLKLDLMDVSGLAERERESRMLELAAEEARRPFDLTRGPLL
                     RAGLVRIGPEEHVLLLTVHHIVSDGWSQGVLVREVGALYEAFSQDKESPLEELPVQYA
                     DFAHWQRNWLAGEVLHKHLTYWQRQLAGDLPALDLPSDWPRPEVQSFRGATLTVTLPS
                     SLSESLSLLCRREGVTLFMLLLAAFKVLLCRYTGQDEIVIGAPIANRNRMEIESLIGF
                     FVNTLVLRTDLSGDPQFSDLLARVRKVTLEAYAHQDMPFELLVEQLQPERSLSRNPLF
                     QVMFQLENTPKEELPMSGLTLSPVEVAGVASQFDLSVDVVESDRGLVVTAEYSTDLFE
                     AETIRTLLKRWRTLLESIAAAPQQRLSELSLIDESERLQLELNWNQSASADSWKCVSE
                     LFEAQVVRSPAAVAVICESDNLTFAELNRRANQLAHYLSSAGVGPEVPVAVVLERSLE
                     IVVALLGILKAGGAYVPLDPSYPQSRLAFMLEDVGVPLILTEESLLAKLPKQHRARVV
                     CMDRDRSAIAAERIENPDRRARLDNVAYVVYTSGSTGTPKGVVITQGALSNHCFAVAR
                     EYELRASDRVLQFASLSFDVATEEIFPTLLAGAAIVLPRERVLDGTELLRLIEKEWVN
                     VLNLPARFWQEWLEGWENEDAKLPECVRLTVAGSEKVSLEGVAIWRRVASAKNTLLNA
                     YGVSEATITSTLYRVNPNLDHVGSADCLPIGRPIANTQVYLLDRHLSPVPIGFAGELY
                     LAGESLARGYHGRLDLTAEKFIPNPFSSRPGARLYRTGDLARYRTDRDIEFIGRVDQQ
                     VKMRGYRIEPGEVEAVLNSAPDVGRAAVLLREGASGDQTLVAYVTQKPAKESIASEAT
                     ARALEAEQLAQWR"
BASE COUNT          981 a         1344 c         1188 g          859 t
ORIGIN      
        1 cgccattgcg cgagttgctc ggcttcgagt gcacgagccg tcgcctccga agctatgctt
       61 tctttggccg gcttttgcgt gacgtaagcg acaagagtct gatctcctga tgccccctca
      121 cgcagcaaca ccgcagcgcg ccccacgtcc ggcgctgaat tcaacaccgc ctcaacctcg
      181 cctggttcga tgcgatagcc ccgcatcttc acctgctggt ccacacgccc gataaactcg
      241 atgtcgcggt ccgtgcggta acgcgctaag tcgcccgtgc gatataaccg cgcgccaggc
      301 ctggagctga acggattagg aatgaacttt tcggcggtca gatccaggcg cccgtgataa
      361 ccacgtgcaa gactttcgcc ggccaggtac aactcgcctg caaaaccgat cggcacaggg
      421 cttagatggc ggtcaagcaa atagacctgc gtgttcgcaa tgggacggcc aatcggcaaa
      481 cagtctgcac tgccaacatg atcgaggttc gggttcactc gatacaacgt cgaagtgatc
      541 gtggcttcgc tgactccgta cgcgttcagc aaggtgtttt tcgcagaagc cacccgtctc
      601 caaatcgcaa caccttccaa cgaaactttc tcactgcctg caactgtaag ccggacgcat
      661 tcaggcaact tcgcgtcttc gttctcccaa ccttccaacc actcttgcca aaaacgagcc
      721 ggcagattca gaacgttcac ccactctttt tcgatcaggc gcagcagttc ggtcccatcc
      781 agcacacgct cgcgcggaag cacgatcgcg gcgcccgcga gcagtgtggg aaagatctct
      841 tcagtggcca cgtcaaaact caacgaagca aactggagca cgcgatccga agctcgaagc
      901 tcatactccc gcgccactgc aaagcaatgg tttgacagcg cgccctgcgt gataacgacg
      961 cccttcggcg ttcctgtcga tcccgacgtg tagactacgt aagcaacgtt atccaacctc
     1021 gctctgcgat ccgggttctc aattcgctcc gcggcaatcg ccgaccggtc gcggtccatg
     1081 cacacgactc gagcccgatg ctgtttgggt agcttcgcaa gcagactctc ctcagtcagt
     1141 atcaaaggga caccgacatc ctccagcatg aacgcgagtc gcgactgtgg atacgaagga
     1201 tcaagcggga cgtaggcgcc gcctgccttc aggataccga gcaaagcgac gactatttcc
     1261 agtgaacgtt caagcaccac tgcaaccgga acttccggtc caacgccggc ggaacttaag
     1321 taatgggcca gttgattggc acgccggttt agctctgcaa aggtcaaatt gtcgctctcg
     1381 caaataacgg ccacagccgc aggcgaacgt acaacctgcg cttcaaataa ttcagagaca
     1441 cacttccatg aatcagcgct tgcgctttgg ttccagttga gctccagctg cagacgctcg
     1501 gattcgtcta tgagcgacaa ttcactcagg cgttgctgcg gtgcagccgc gatactttcc
     1561 aacaaagtcc gccagcgctt gagaagagtg cggatcgtct cagcctcgaa aagatctgtg
     1621 ctgtactccg ccgtgaccac gagccctcga tcgctctcga cgacgtcgac actgagatcg
     1681 aactggctag caacgcctgc gacctccacc ggactcaacg tcaaccccga catcggcagt
     1741 tcctctttcg gggtgttctc gagttgaaac atcacctgaa acagcgggtt gcgactgaga
     1801 ctgcgttccg gctgcaactg ctcgacgagc aattcaaaag gcatgtcctg atgggcgtaa
     1861 gcctcgagtg tcactttgcg cacgcgcgcg agcaggtccg agaactgcgg gtcgccggag
     1921 aggtcagtgc gcaacacaag cgtgttgacg aagaaaccga tcaggctttc gatttccatg
     1981 cgattgcgat tcgcgatagg cgcgccgatg acgatctcat cctgccccgt gtagcgacat
     2041 agcaacacct tgaaagccgc gagcaacagc atgaagagtg tcacaccttc tcgccggcac
     2101 aggaggctga gcgattcgct caaactggac ggcaatgtca cggtaagcgt cgcgccgcga
     2161 aagctctgca cctccgggcg cggccagtcg gatggtagat ccagcgccgg cagatcgccg
     2221 gcaagttgtc gttgccagta tgtgaggtgc ttgtgcaaca cttcacccgc cagccagttg
     2281 cgctgccagt gcgcgaagtc ggcgtattgg accggcagct cttcgagtgg agattctttg
     2341 tcctggctaa atgcttcgta gagagcgccg acctcgcgca ccaaaacgcc ctgcgaccac
     2401 ccatctgaaa cgatatggtg gaccgtcaac aacagcacgt gttcttccgg tccaatccgc
     2461 acgagaccgg cacgtaacag gggcccgcga gtcaaatcga atggccgacg cgcctcttcc
     2521 gcggcgagct ctaacatgcg cgactctcgt tcacgttcag ccaacccgct gacgtccatc
     2581 agatcgagtt tcagctgcgg cggtggtcca acaaactgcg ccggctgacc atcgatcatg
     2641 cggtagtaag tacgcaggat ctcatgtcgc ctgacaactt cgttcagcgt gcggctgagc
     2701 gccgtgatat cgagtggacc gctaagacga actgccgtgg gaatgttgta tgcggacgtg
     2761 ctcggctcca actgataaat gaaccagagc cgttgttgcg agaatgagag cgggagattg
     2821 ccattacgag gcacgggaac gatcggcgga gcgtgtaaac cgttttccgt gcctagcgtt
     2881 gcttcgactt gtgcggcctg ttcggccagc gtggctgact cgaagaacag tttcagcggc
     2941 aactccactc cgaagtgcgc ctgcgcacga gaaacaagac gcgtcaccag cagcgagtgc
     3001 cctccgagtt cgaagaagtt atcgcttaca ctaacgcgct cgactttcaa cacatcgctc
     3061 caaataccgg ccagtaactc ttcgaccggc gttcgcggcg ccgtatgagc tgattctgac
     3121 gctgcgtgaa tttcatcggg cgcgggcaac gcccgccggt caacctttcc gtttggcgtc
     3181 agtggtagtt catcaagaat gacgaacgcc gatggcatca tgtaatcggg caaagtcgat
     3241 cggagggtct tttgcaacgc ttccacagag acacgatcag cagcctcgac gtaagcaacc
     3301 aaacgtttgt cgccgccgcc atagtcgagc gccaccaccg ccacttgccg cactcgcggc
     3361 acgcgactga gtgccgtctc cacttcgccg agctcgacac gataaccgcg aaccttaacc
     3421 tgttcgtccg cccggccgat aaactcgatg ttgccgtccg ccaggtagcg tacccggtcg
     3481 ccggtgcgat aaagccgcgc tcccggctcg ttactgaatg ggtgtggaat aaagcgttcg
     3541 gcagtgagac tcgcgcgtcg gtgataacca cgcgccagac catcgcctgc cgcatacaac
     3601 tcgccaacga aacccggcgg agataattgc aaattctcgt ccaggataaa gacctgggtg
     3661 tttgcaatcg ggcgcccgat gggcactgag ccgttgaagt caacctgctg atccatcacg
     3721 tagcagcaag tgaacgtagt gttttccgtt ggaccatagc cattgattag cctgcacgag
     3781 ggagcggcgg ttacgaattt ctttacctga gatggcgaga gaacgtcgcc tccggccaat
     3841 agctgtctga gcgcagcaag agcttcgggc tgctcgtcaa ccatttgatg aaataagccg
     3901 gccgtcaacc acaaggtagt cacacgatac cgcgtgactg cacgcccgag atcttccagc
     3961 gaggctacgc cggaggacat gagcacaagt tgcgctccgt taagcaggct gccccagatt
     4021 tcgaaagtcg acgcatcgaa ggcgagcggt gcaaactgta agaagacctc actgggaccg
     4081 aaattcgcat agtcgtcgcc agtgaccaga cgcacgacgc cacggtgcgg tacctccact
     4141 cccttaggag taccggttga gcccgacgta tagacgatgt aagcaaggtg ctcgggcgtg
     4201 atccttccct cggggttctc ttcactgtgc cccacgatca aactccagtc agaatcgagc
     4261 aaaacgaccg gcgctcgatg cgcgggcagt tttccctgca atgccgcgac actgagcacc
     4321 aactgtacgt cagcgtcatc gagcatgaat aacaatcgtt cttccggata ca
//
LOCUS       DASTEY010000280         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_39092, whole genome shotgun sequence.
ACCESSION   DASTEY010000280 DASTEY010000000
VERSION     DASTEY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39092"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1431)
                     /gene="glyA"
                     /locus_tag="VFO72_04910"
     CDS             complement(160..1431)
                     /gene="glyA"
                     /locus_tag="VFO72_04910"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9478658.1"
                     /translation="MNRNSSLAEVDPIIAGAIDNEVARQANGLELIASENFVSEAVLE
                     AAGSVFTNKYAEGYPGKRYYGGCEWTDVVEQTAIDRLKELFGAEHANVQPHSGAQANT
                     AVYLAAIQYGDQILGMSLAHGGHLTHGHPLNFSGLSYKVADYGVSKETEQIDYDELQR
                     LAEEHRPKLLVCGASAYPRIIDFERIGQIARSVGARMFADIAHIAGPIAAGLHPSPVP
                     HADYVTSTTHKTLRGPRGGLILCREEHAKDIDRRVFPGVQGGPLVHIIAAKAVAFGEA
                     LREDFKEYQRQVLANAKALASELQEQGLRLVSGGTDNHLMLVDVWLDGKGVTGKDAEK
                     ALEAALITVNKNTIPFDQNKPFVASGLRIGTPAVTTRGMKEPEMREIGRLIAEVIHAP
                     ESEEVRNKVKRDVADLTARFPLYSKRMKAAG"
     gene            complement(1428..1871)
                     /gene="rpiB"
                     /locus_tag="VFO72_04915"
     CDS             complement(1428..1871)
                     /gene="rpiB"
                     /locus_tag="VFO72_04915"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9478659.1"
                     /translation="MRIALASDHAGYAEKERLKGLLVDLGIEFDDLGTVSEESVDYPD
                     YARKVAEQVAQGRVEQGVLVCGSGTGMAITANKVPGVRAAVAWSEEIARLARQHNDAN
                     VLAIGARTTPPAEIPKIVRAWFETEFEGGRHASRVEKISEVERSR"
     gene            2072..>2443
                     /locus_tag="VFO72_04920"
     CDS             2072..>2443
                     /locus_tag="VFO72_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478660.1"
                     /translation="MRWSRYFIPTLREDPADAEVISHKLLLRAGVIRQLSAGIYSLLP
                     LGQRIALKVMQILREEMNAIGGQEFYLPALHPAEIWQESGRWDAIGDEMFRLKDRKGT
                     DMCLGMTHEEVFTTIARNEIRS"
BASE COUNT          498 a          749 c          654 g          542 t
ORIGIN      
        1 gtacccgtcc cggcctcgac aatcaaatgc ctcttctcct caaacgcgcg catcaccgcc
       61 cgcgccatct caatctggcc cggccgatgt tcatattcag gatgcgcccg cgcaatcagt
      121 ccatccggac caaaaacttc aagcatatga tccgtgttgt tatcctgcgg ccttcattcg
      181 cttcgaatat aacggaaacc tcgccgttag atccgctaca tcacgcttaa ctttattcct
      241 aacctcttcc gactccggtg cgtggatcac ttccgcgatc agacgaccaa tctcgcgcat
      301 ctctggttcc ttcatgccgc gagtggtgac cgcgggtgtg ccgatgcgca aaccggaggc
      361 gacgaatggt ttgttttgat cgaagggaat cgtgttcttg ttgacggtga tcaacgcagc
      421 ttcgagggct ttctccgcgt ccttgcccgt gacgcccttg ccgtccagcc aaacgtcaac
      481 cagcatcagg tgattgtcag tgccgccgga gacgagccgc agtccctgct cctgtaattc
      541 actcgccagc gcttttgcat tcgctaaaac ctggcgctgg tattctttga agtcttcacg
      601 caacgcctcg ccgaacgcca ccgccttcgc agcgatgatg tgtacgagcg ggccgccctg
      661 cacgccggga aaaaccctgc gatcgatgtc tttcgcatgc tcctcgcggc agaggatcaa
      721 gccaccacgt ggaccacgca aggttttgtg agtcgtcgag gtgacgtagt cggcgtgtgg
      781 caccggagaa ggatgcagtc cggcggcaat tggtccggca atgtgcgcga tgtcagcaaa
      841 catgcgcgcg ccgacactgc gcgcgatctg gccgatgcgt tcgaagtcga ttatccgcgg
      901 atatgcagaa gcaccacaga caagcagctt gggtcgatgc tcttccgcga gcctctgtaa
      961 ctcgtcgtag tcgatctgtt ccgtctcttt ggaaacgccg tagtcggcga ccttgtaact
     1021 gagtccggaa aaattcaaag gatgcccgtg agtcagatgg ccgccgtgcg caagactcat
     1081 ccccaggatc tgatcgccat actggatcgc cgccaggtag accgccgtgt ttgcctgcgc
     1141 gccactgtgt ggctggacgt tcgcgtgttc cgcgccgaac aattccttca agcgatcgat
     1201 cgcggtttgc tcgacgacgt cagtccattc acatccgccg tagtaacgtt tacccggata
     1261 accttcagcg tacttattcg taaacaccga tcccgcagct tcgagcactg cctcactgac
     1321 aaagttttca gaggcgatga gctcgaggcc attagcctgg cgtgccactt cgttgtcgat
     1381 ggcgccggcg atgatcggat cgacttcggc gagtgatgag tttctgttca tctgcttctt
     1441 tccacctcac taatcttctc cacgcgcgag gcatgacggc caccttcaaa ttccgtctca
     1501 aaccaggcac gcacgatctt tggtatctcg gcgggcggcg tggtgcgagc gccgatggca
     1561 agcacgttcg catcgttgtg ttgtcgcgcg agtcgtgcaa tttcttcgga ccacgcgaca
     1621 gctgcgcgca cgccgggaac cttgttggca gtgatcgcca tgccagtgcc cgagccacac
     1681 accaatacgc cttgttcgac ccgtccctgc gccacctgct ccgcaacttt ccgcgcgtag
     1741 tccggataat ccacggactc ttccgacaca gtgccgagat cgtcaaactc aatgccaaga
     1801 tcgacgagca gccctttcaa gcgctccttc tcagcgtaac cagcatgatc gcttgcgagt
     1861 gctatacgca tgtttcgaaa acctcgttag gcggtgtgta aagaagaagc gtgatggtaa
     1921 ctcaggcgtc aatgggcagc aaactccttg gagtgcggtg ccttggcacc gctttggcca
     1981 aatgtgtcga atagaagaga ccaaggcacc gcactccagg gaggcgccca tgttgcctta
     2041 accgcgctcg ctaagcgaga atgccaaacc tatgcgctgg tcacgatact ttatacccac
     2101 tctacgcgaa gacccggctg atgccgaggt catttcgcac aagcttctct tacgcgctgg
     2161 tgttatccgg caattgtcgg ccggaatcta ttccctgctc ccgttaggcc agcgaatcgc
     2221 actcaaagtc atgcagatcc tgcgtgaaga gatgaacgct atcggcggtc aggagtttta
     2281 cctgccggct cttcatccgg ctgagatctg gcaggagtcg ggacgctggg acgcgatcgg
     2341 cgacgaaatg tttcgactca aggaccgcaa aggcacggac atgtgcctgg gcatgacgca
     2401 cgaagaagtc tttaccacca tcgcgcgcaa cgagattcgc tcg
//
LOCUS       DASTEY010000281         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1555195, whole genome shotgun sequence.
ACCESSION   DASTEY010000281 DASTEY010000000
VERSION     DASTEY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555195"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFO72_04925"
     CDS             complement(<1..212)
                     /locus_tag="VFO72_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478661.1"
                     /translation="MKQLVKTLVFLLAFYSMCAAQQIVDKMVATVNAGVRTELITYSD
                     LMWQLALQPNTQLDNPNSEQLNRALRL"
     gene            complement(220..3591)
                     /locus_tag="VFO72_04930"
     CDS             complement(220..3591)
                     /locus_tag="VFO72_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET9478662.1"
                     /translation="MKQLFSEAPNIRLLLILLLVLLFQGSALGNTARDARIEDYEGRQ
                     ITAVEVVIEGSAENPNVQAELLALLRVAPNTVFSAVRVRDSLQALFDSGRVGNARVEV
                     VEAGATRTGPVRLRFVVKRQVQIGDVRIELGPYTGTPLSVDELRARLNFVQPLAPLSQ
                     QLILRNADEIQVYLRDRGYFNAVVEPLEQVGPRGVRATVTYRVTPGEQARVEAFDIAI
                     KGFETSRVRNSLTLQPGVAFTRDDLATDVNKIRDALIAARFLSPVLEDPRVERDAEKN
                     TVQISLTGAVGPTVQVSVTNYEISEKAQRELLPVKREGNIDYSAIVEGARRIRNRLQE
                     DGYFFAEVTEICRVNNPPPELGSNGTQETCQNLNPTVLSGHNVEIEYQVERGRRFRLT
                     DIRITGTNKLTYDDIAANLKTQKQNAIGLIPFLGYGRGYTSVTLLEQDRRTVEAFMRD
                     LGYRKVKAEVLQGVSIDGENLIITFQVTEGPLTRIAGVDVKGNQIYTEERIRQELTTV
                     IGAPYSRSHARSDADKVAQLYSREGYINARVDISIVELPVKGDDEQVRLIYTIADEGD
                     KVFINQILINGVTGSRNTRRVKRDAIRRAIPLAEGDVLRADRISEAERELYLTDAYRQ
                     VIIRTEAAGETPGGYKRRDVIIDVEEKQPNVMDYGGGYSTDAGALGLFEISKVNLMNR
                     LRQGAIRLRASRLQQRVRLEYLDPRFARYNKRQFAPLAASIEYQRDSTITRFFRSAID
                     RGTMGIIQRLDEEGNAIDIFGNQVNEPTINRFTAALETQRVLDRDTRTIVFARYSYED
                     VRLFNLESLVVRPILEPDSKIRLSRFGVSFVRDTRERCEKDLLARSVDERDEDEQPGA
                     PGEVCRYNQVDATRGGFLSVDYALALRQLGGNISFNRFQATYRHYYKMSALRDTVFAS
                     NFTLGLANLFNPRDRDGNGSIDEIDRTLPISERFFSGGSTTLRGFNFEEAGPREVVIP
                     EGEFRDQDKNIVFLNPFTVPVGGNALAIVNLEARIPLTKSVQVVPFYDGGNVFRRVGD
                     LFGRDDPAPVPPGDILAAIRAVNLRAKWTNTAGLGFRWQTPFGGVLAVDYGFLLKPPE
                     FLIPQRGPDGISFDGTPAVFRLHRGNLHFRITQTF"
BASE COUNT          811 a         1081 c         1012 g          844 t
ORIGIN      
        1 agccgaagcg ctcggttcaa ctgctcagag ttcggattgt ccagttgcgt gtttggttgc
       61 aacgccaact gccacatcaa atcagaataa gtgatcaact ccgtcctcac gccggcatta
      121 accgtcgcca ccatcttgtc gacgatctgc tgcgccgcac acattgaata aaacgccagc
      181 aggaaaacta aagtttttac aagttgcttc atatacggct caaaacgttt gcgtaatcct
      241 gaaatgcaag ttacctcgat gtaagcgaaa caccgccggc gtgccgtcga acgaaatgcc
      301 gtctggccca cgctgcggaa tcaagaactc cggtggtttc aaaaggaatc cataatcaac
      361 agccaacacg ccaccgaacg gcgtctgcca gcgaaatcct aagcctgctg tgtttgtcca
      421 cttcgcgcgc aggtttactg ccctgattgc cgcgagaata tcaccgggcg gaacaggcgc
      481 cggatcgtct ctgccaaaca gatcgccgac tcggcgaaac acgttgccac catcgtaaaa
      541 cggcaccacc tgcaccgact tcgtcagcgg gatgcgagct tcgagattca cgatcgccag
      601 cgcgtttccg cctaccggca ctgtaaaggg attgagaaaa acaatgttct tgtcctggtc
      661 gcggaattca ccttccggaa tcacaacctc tcgtgggccg gcctcttcga aattgaaacc
      721 gcgcagagtt gtcgaaccac cggagaagaa tcgttcgctg atgggtagcg tgcgatcgat
      781 ctcatcgatg cttccgttgc cgtcgcggtc cctcggattg aagaggttcg cgagaccgag
      841 cgtgaagttg ctggcaaaaa cggtatcgcg cagcgcgctc attttgtagt agtgacgata
      901 cgtcgcctga aaccgattga aagagatgtt cccgccgagt tgccgcagcg ccaacgcata
      961 atcaacactc aagaagccgc cacgcgttgc gtcgacctgg ttgtagcggc aaacctctcc
     1021 gggtgcgccc ggctgctcgt cctcatcgcg ctcgtctacg cttcgagcga gcaggtcttt
     1081 ttcacaacgc tcgcgcgtgt ctctgacgaa cgaaacaccg aaacgcgaca gacgaatctt
     1141 gctgtcgggc tccaggatcg gcctgacaac cagactttca aggttgaaca ggcgaacgtc
     1201 ttcatagctg taccgcgcaa agacgatggt gcgcgtatcc cgatcgagca cgcgctgcgt
     1261 ttccagggcc gccgtgaagc gattaatcgt cggttcattt acctggttgc cgaagatgtc
     1321 gatcgcgtta ccttcttcgt ccaaccgttg gatgattccc atcgtgcccc gatcgatcgc
     1381 tgaacggaag aaacgcgtga tcgtcgaatc gcgctggtac tcaatcgagg ccgccagggg
     1441 cgcaaattgc ctcttgttgt atcgagcaaa ccgcggatcg agatactcga gccggacgcg
     1501 ttgctgaaga cggctcgcac gcaaacggat tgcgccctga cgcaaccggt tcatcaggtt
     1561 gactttgctg atctcgaaca agccaagcgc gcccgcgtca gtggagtaac cgccgccgta
     1621 gtccatcaca ttcggctgtt tctcctcgac gtcgatgatc acgtcgcgtc gtttgtaacc
     1681 accgggcgtt tcgccggcag cctcagtgcg aatgatgacc tgacggtaag cgtctgtcag
     1741 ataaagttcg cgctccgctt cggaaattcg atcggcccgc aggacgtcgc cttcggcgag
     1801 cgggatcgct cgtctgatcg catcacgctt tacgcgcctc gtgtttcggc tgcccgtaac
     1861 gccgttgatg agaatctgat tgatgaaaac tttgtcgcct tcgtcggcga tcgtataaat
     1921 cagacggacc tgttcgtcat cacctttgac cggcagttcc acgatagaaa tatcgacgcg
     1981 cgcgttgatg taaccttcgc gcgagtagag ttgggccact ttatcagcgt cggagcgtgc
     2041 gtgtgagcgt gagtaaggcg cgcctatgac agtcgtaagc tcctgacgaa tcctttcctc
     2101 cgtataaatc tggtttcctt tgacgtccac gccggcgatg cgtgtgagcg gtccttccgt
     2161 tacctgaaac gtgatgatca gattctcgcc gtcgatcgaa acgccctgaa gcacttcagc
     2221 tttcactttg cgataaccca gatcgcgcat gaacgcttct acagtccggc ggtcctgttc
     2281 gagcagcgtg acgcttgtgt acccgcgtcc gtacccgaga aacggaatca ggccaatcgc
     2341 gttttgcttt tgagtcttca agttggcggc gatgtcgtcg tacgtcagct tgttggtgcc
     2401 ggtgatgcga atgtcggtca aacgaaatcg cctgcctctt tcgacctgat actcgatttc
     2461 gacgttgtga ccgcttagga cagtcggatt caagttttgg caggtttcct gggtgccgtt
     2521 tgatcccaat tccggagggg gattgttgac acgacaaatc tcggtcactt ccgcgaagaa
     2581 gtaaccgtct tcctgtagcc ggttgcgaat gcggcgcgcg ccttcgacta tagcggagta
     2641 atcgatgttg ccttcacgtt tgaccggcaa caactcgcgc tgcgctttct cgctgatctc
     2701 atagttcgtg accgagactt gaacggttgg tccaacagcc ccagtcaaag agatctgcac
     2761 ggtattcttt tccgcatctc tttcgacgcg cggatcttcc agtaccggcg agagaaaccg
     2821 cgccgcgatc agcgcatcgc ggattttgtt cacgtcggtg gcaagatcat cacgcgtgaa
     2881 ggcgactccg ggctgcaacg taagggagtt acgaacgcga gacgtctcga aacctttgat
     2941 tgcgatatca aatgcttcga ctcgcgcctg ttcgccggga gtgacgcggt aagtgaccgt
     3001 ggcgcgaacg ccgcgagggc cgacctgttc gagcggttcc acaacggcgt tgaagtaacc
     3061 gcgatcgcgc aaatagacct ggatttcgtc ggcgttgcgc aggatcagtt gttgtgagag
     3121 tggagcgagc ggttggacga agttcaatct agctcgcagt tcgtcaaccg agagtggagt
     3181 tcccgtgtaa ggccccaact cgatacgcac gtcgccgatc tgtacctgtc gtttgactac
     3241 aaatcgaagc cgcaccggtc cggtgcgcgt ggcaccggct tcgacaactt cgacgcgcgc
     3301 attgccgacg cgtccggaat caaacagcgc ctgaagcgaa tcgcgcacac gaaccgctga
     3361 aaacactgta ttcggcgcga cccttagcag cgccagaagc tcagcctgca cgtttggatt
     3421 ttccgctgaa ccttcaatga cgacttcaac cgccgtgatt tgccggcctt cgtagtcttc
     3481 aattcgcgca tctcgcgccg tgttgcctaa ggcgctgccc tgaaacagaa gaaccagcag
     3541 cagaatcaac agcagtcgaa tattcggagc ctccgagaac aattgcttca cttacccctt
     3601 gctcaaaatc gctttctaaa ccggatctca aaattaaagt tgttcgtgcg cgcgctcagt
     3661 ctgcgcgtgg atgcctgttc gtattgcgca ataaacgaca gtcgatccga aacgcgatac
     3721 tcgagcgaga gtatctggtt cggatccg
//
LOCUS       DASTEY010000282         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_137901, whole genome shotgun sequence.
ACCESSION   DASTEY010000282 DASTEY010000000
VERSION     DASTEY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137901"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..>2097)
                     /locus_tag="VFO72_04935"
     CDS             complement(125..>2097)
                     /locus_tag="VFO72_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET9478663.1"
                     /translation="HPDRLLYPLIKRNGRFERASWDEALDLVVTKFKELQEKYGKDSI
                     GVYSGSSLTTEKTYLMGKFARAGLGTRYVDYNGRLCMVSAAAGNNKAFGIDRAANPWS
                     DIPLAEVLLIAGSNCAETFPVLNKFLWQQRDNGGLWIIVDPRETPTARQGDLHLQLKP
                     GTDVALANGILHVLIEENLIDQAFIDSRTNDWQATRELAARYSPDVASQICSVPAQKI
                     IDAARLYGRAKTAMVMHARGIEHHTNGVNNVLSYINLVLATGKIGAPGRGYGTITGQG
                     NGQGGREHGQKADQLPGQRSIMNPEHRKHVCQVWDLPEAELPQAGVSVVEMFEKMREG
                     EIRGLLSICNNVMVSLPDTNDVRRSLEGLEFYVCIDFFMSEGARYADVVLPGSAWSED
                     EGVTCNSEGRVVKINKANNPPGEARSDWWIVQEIARRMGREKYFAFNSPREIFTELRV
                     ASRGGNADYYGITWEKIEKNNGIFWPCPTEDHPGTPRLFEDRFYHPDGRAKFHAIEYN
                     PPKEVPDEEFPLILTSGRVVYQYLSGNQTRRIGFLVEQCPEPYVEVHPETAAKLKIND
                     GERVRVVSRRGDGVFPVLVVRTIRPDTIFIPYHWGEQLAVNQLTNPALDPTSKIPEFK
                     ACAARIEKIPSRQLPILGRQRKGASASEVQRG"
BASE COUNT          441 a          600 c          559 g          497 t
ORIGIN      
        1 gatcgatata gtcgacgaag atcatcgaat agcctttatg cgtggcgcat tcgcgacaag
       61 cggcaacaca ggcgcggcag cctatgcagc gttgcggatc gataaacatc gtctcttcca
      121 tccgctaccc cctttgcacc tcgctggccg atgcgccttt gcgctgcctg ccgagaatcg
      181 gcaattgtcg cgaaggaatc ttctcaattc gcgccgcaca ggccttgaac tcgggaatct
      241 ttgaagtcgg atccagtgcg gggttcgtca attgattcac tgctagttgt tctccccagt
      301 gataggggat aaaaatcgtg tcgggacgaa ttgtccgcac caccaacact ggaaacacgc
      361 cgtcgccgcg ccgcgagacc acccgcacac gttcgccatc gttaattttc agtttcgccg
      421 ccgtttcggg atggacttcg acatacggtt caggacattg ctctaccaga aaaccaatac
      481 gtcgcgtttg attacctgaa agatactgat agaccacgcg tccactagtc agtatcaacg
      541 gaaactcttc gtcaggaact tctttcggtg ggttgtattc aatcgcatga aacttcgccc
      601 tgccgtccgg gtgatagaag cgatcttcaa acagacgcgg cgtacccgga tgatcttcgg
      661 tcgggcaagg ccagaagatg ccattgttct tttcaatctt ctcccaggtt atgccgtagt
      721 aatcggcgtt gccgccgcgc gaggcgacac ggagctctgt gaagatttcg cgcggcgagt
      781 tgaaagcaaa atacttctcg cgacccatgc gccgagcgat ttcctgcacg atccaccagt
      841 cagagcgagc ctcgccaggc ggattgttgg ctttgttgat ctttacgacg cgtccctcgc
      901 tattacacgt gacgccttcg tcttcgctcc aggctgagcc gggtaaaacc acatccgcat
      961 aacgcgctcc ttccgacatg aagaagtcga tgcagacata aaactccaga ccttcgagtg
     1021 agcgtcgaac atcgttggtg tccggaaggc ttaccatcac gttgttacag atcgacagca
     1081 agcctcgaat ctcgccttcg cgcatcttct cgaacatctc aacaactgag actccggctt
     1141 gtggtagctc agcttcgggc aagtcccaaa cctgacacac gtgtttgcga tgttccgggt
     1201 tcatgatcga acgctgcccg ggaagttgat ctgctttttg gccgtgttcg cgtccgccct
     1261 gaccgttgcc ctgaccggtg atcgtcccat aaccgcgtcc gggcgctcct atcttccccg
     1321 tggccaggac caggttgatg taagacagta cgttgttgac gccgttcgtg tgatgttcaa
     1381 tgccgcgtgc gtgcatcacc atggctgtct tggcgcgacc atagagtcga gcagcgtcta
     1441 tgatcttttg ggctggcacg ctgcaaatct gcgaggcgac atcgggtgaa taacgcgcgg
     1501 ccagttcgcg cgttgcttgc cagtcgttcg tgcgtgagtc gatgaaagcc tgatcgatca
     1561 ggttctcttc gatgagaacg tgcaggattc cattagcgag tgcgacgtcg gtgcctggct
     1621 taagctgaag atgcagatct ccctggcgcg ctgtcggcgt ctcgcgtgga tcgacgatta
     1681 tccagaggcc gccgttgtcg cgttgttgcc agagaaactt gtttagcaca ggaaaagtct
     1741 cggcacagtt ggagccggcg atcagcaaaa cttccgctag cggaatgtca ctccacggat
     1801 tcgcggcgcg atcgatgccg aatgctttgt tgttcccagc cgccgccgag accatgcaga
     1861 gtcgcccgtt gtaatcgacg taacgagtac ctagtcccgc acgcgcgaac ttacccatca
     1921 ggtaagtctt ctccgtcgtc aaagaagatc cggagtagac tccgattgag tctttgccat
     1981 acttctcttg cagctctttg aatttcgtga cgacgagatc cagcgcctcg tcccaacttg
     2041 cgcgttcaaa acgaccattg cgtttgatca gcggataaag cagacgatcc gggtgat
//
LOCUS       DASTEY010000283         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1538709, whole genome shotgun sequence.
ACCESSION   DASTEY010000283 DASTEY010000000
VERSION     DASTEY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538709"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VFO72_04940"
     CDS             complement(<1..1055)
                     /locus_tag="VFO72_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478664.1"
                     /translation="MLESSRESIYAVDPNGNFKWCNTATLQGLGYQRSNFIGRPLLSM
                     VYEGDRELVKEKLDAALTGAPQTYEMRYFSRDGQLRYARVDNSPLVVDGRTTGVLGIA
                     RDITEQKEERERAARADKLRALGQLASGVAHDFNNSLAAILGRAQLLRRQIVEPALVR
                     NLDIIQTAAEDAAATVRRIQTFARKSPVKEFELVDVGSLLNDAVEITRTRWQNEARVR
                     GLEYEVNLDTATGINTYGSASELREVFVNLIVNAVDAMPKGGKLFITCRCHDGRLQLT
                     FKDNGMGMPEDVRQKIFEPFFSTKGAHGTGLGLSVSYSIIERHEGSISVLSAPGKGTE
                     FTIDLPAVMAESSTPDAS"
     gene            complement(1077..>2423)
                     /locus_tag="VFO72_04945"
     CDS             complement(1077..>2423)
                     /locus_tag="VFO72_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9478665.1"
                     /translation="HEIYQTAVKELGSYLAVDRCSLFMRDDRAKCATNVAEYHNEGVQ
                     PAGADFPLAHLQTLVTALDKTGVLPFSDAANDARITDLYQNILSKANVKSIMYVAIRV
                     GDDVPAAFALSTTRELREWSEADIALAKAVADQTGIAIKQAELYQKAETTSIREALVN
                     RLTMAIRASLSLSEVLRTATVELGKALGASRVHLFLSNSDGVDVPVEHEYLAPDVASA
                     KHVAFDYDNPISRRLASSSKSLIIDDSLNPDDSSTVLRDYVRAHAQRFDIRSQINYPL
                     MVKGQFRGVLSIHQTDRKRLWAEDEVDLVEALAERLAIGIAQAELFEMVARGKTEWET
                     TFDAMSDGIFIFNRVGVLSRVNRAGATMEAVHPRLLLGRQCCDILRTSDEDETCVVEK
                     ALETGRSVTIEITPNRLNRPLLVTVEPVLDENNRAQGVVCTARDLSDLRMVQAVAR"
BASE COUNT          526 a          701 c          646 g          550 t
ORIGIN      
        1 gacgcatcgg gtgttgaact ctcagccatt acggcaggaa gatcgattgt aaactccgta
       61 cctttccccg gtgcagacag aacgctaatc gaaccttcgt gacgttcgat aatgctgtag
      121 cttaccgata gccccagacc agtcccgtgg gcgcctttag tcgagaagaa aggttcgaag
      181 atcttttgtc gcacgtcctc cggcatgccc atgccgttat ctttgaatgt tagttgtagc
      241 ctgccatcgt ggcaccggca ggtgatgaac agctttccgc ccttgggcat cgcatccacg
      301 gcgttcacga tcaagttgac aaaaacttcg cgcagctcgg aagcgctgcc gtaagtgttg
      361 attccagtgg cagtatcgag attgacctcg tattccagcc cgcgaacacg cgcctcattc
      421 tgccagcgag ttctcgttat ctcgaccgcg tcgttaagca aactaccaac atccactaac
      481 tcaaactcct tcaccggcga cttgcgcgca aaagtctgaa tgcgccgcac tgtcgctgcc
      541 gcatcttcgg cggccgtttg aatgatgtcg agatttcgga ccagcgccgg ttcgacgatc
      601 tggcggcgca gcaactgtgc acgcccgaga atcgcagcca gcgagttgtt aaaatcatgg
      661 gccacgcccg acgcgagttg tccaagtgca cgcaacttgt cagcgcgtgc agcacgttcg
      721 cgctcttctt tctgctcggt gatgtcgcgc gcaatgccca acacgcccgt ggtccgtcca
      781 tctaccacta gcggagaatt gtctacgcgt gcgtagcgga gctgtccatc gcgagagaag
      841 tagcgcatct cgtaagtttg cggcgcgcca gttaaagctg catccagttt ctctttaacc
      901 aactcacgat cgccttcata gaccatactc agcagtgggc ggccgatgaa gttgctgcgc
      961 tgataaccga gaccttgcaa ggtcgctgtg ttgcaccact tgaaattacc gttcggatcg
     1021 actgcgtaaa tcgactcccg agagctctcg agaatgtttg tgagtagcga ttgatgctag
     1081 cgtgcaactg cctggaccat acgcagatcg gaaagatcgc gcgcagtgca aacaacgcct
     1141 tgcgctctgt tgttttcgtc aagaaccggt tcgactgtga cgagcaaggg ccggttaaga
     1201 cggttcggag tgatttcgat cgtgacgctg cggccggttt cgagtgcttt ctccaccacg
     1261 cacgtttcgt cttcgtcgct ggttcgcagt atgtcgcaac actgccgccc aaggagcagc
     1321 cgcggatgaa cggcttccat cgtggcgccc gcacgattca cgcgactcaa cacaccgacg
     1381 cggttaaaga tgaagatgcc gtcagacatc gcatcgaaag ttgtttccca ttccgttttg
     1441 ccgcgcgcga ccatttcgaa cagttcggcc tgggcgatgc cgatggccag ccgttctgcc
     1501 agtgcttcga cgaggtccac ttcatcctcg gcccacagcc tctttcgatc ggtttgatga
     1561 atggaaagta cgccgcgaaa ctggcctttg accatcaacg gatagttgat ctgcgaacga
     1621 atatcaaaac gctgcgcgtg ggcacgcaca taatctctca gcaccgtcga actatcatca
     1681 ggattgagag agtcgtcgat gatgagcgac ttactcgaac tcgcaagacg gcggctgatg
     1741 ggattgtcgt agtcaaacgc aacgtgcttg gcgctcgcaa catccggcgc gagatattcg
     1801 tgctccaccg gcacatcaac cccgtcgctg tttgacagaa atagatgaac tctcgacgcg
     1861 ccaagcgctt tccccaattc aacagtggcc gtgcgcagaa cttccgacaa actcaacgaa
     1921 gcgcgaatcg ccatcgtcag ccggtttacc agtgcttcgc gaatcgacgt ggtttccgcc
     1981 ttctgataaa gctcggcctg cttgatcgcg atcccggttt gatctgcgac ggctttcgct
     2041 aaagcgatgt cagcttcact ccattcgcga agttcccgag tcgtggaaag tgcaaatgct
     2101 gcgggcacat catcgccgac gcgaatcgcc acgtacatga tcgatttgac gttcgctttt
     2161 gaaagaatgt tctggtagag gtcggtaatg cgggcatcgt tggccgcatc cgaaaatggc
     2221 aagactcccg tcttgtcgag agccgtaacc agagtttgca agtgggcgag tggaaagtcg
     2281 gcgcctgccg gttgcacgcc ttcattgtga tactcagcca cgttagtcgc acacttcgcg
     2341 cggtcgtctc gcatgaacaa agaacagcga tccacagcga gataggagcc cagctctttc
     2401 acggccgttt gatagatctc gtg
//
LOCUS       DASTEY010000284         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_862994, whole genome shotgun sequence.
ACCESSION   DASTEY010000284 DASTEY010000000
VERSION     DASTEY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862994"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="VFO72_04950"
     CDS             <1..1810
                     /locus_tag="VFO72_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9478666.1"
                     /translation="IFGTRLPDTGFTQYGGLLRANLNLDDNTQLLLSYARNQQDNGKR
                     YDQLLGGDGNNIADLRNLMTDTFYIRLFRQKFGIFDNGSFTFSYNGQREERINQGGQG
                     NPAAAITNQRERTNVLGFNFYLDKQMGDRNTLLFGADLYHDKIKAPAFTFDPVTRTTS
                     NSRPRIPNGARYVSSGVYAQNNFTAIPDRLQINGAIRYSVASYVSRAANSAPGPGGLP
                     LFPDDSARFDAFSGRIGGVVNVAGGFDIAGKYTRGFRAPNTTDLGIVGLVGTGFEVDA
                     NTASARGGFIGTTSGGDAISTGVPVMSLGPEFSDSFDLSFRYTSQHFGAELTGFTTKL
                     SDVYFDQALVLPPGATGTFLGTEQIIAQNEAGLVFVAAAPTSPVLVRVNFDDARISGI
                     EFNTRARFNDQFSGNGNFTYVRAHSLLNGLPPSVEAGIPPATAFVSFRYQPRPRVYLE
                     AYSTLAGRQNRLSSLDLADRRTGAERSRNNIQNFFRRGACVRGLTTPGPTGCGSAGGI
                     LIPTGETLVQVQDRLLPIGAVINGVRVVNNDTLVPLFPYLPGYGLVGVRGALKFGETS
                     EVFVDFENILDKSYRGISWGIDGAGRGVTVRYRYSF"
     gene            complement(1811..>2435)
                     /locus_tag="VFO72_04955"
     CDS             complement(1811..>2435)
                     /locus_tag="VFO72_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET9478667.1"
                     /translation="LLTGNSVASLSVAMNASGETYTTEGGLISQVFISSAGYLGSMAF
                     GALLLVLIRKAIAARIVLLSSAVLIFGLTMIYGLLKPMFSLTAWSGIPFTLLAGTFLS
                     LGLVLIARFATARVATFFVSFLAVQCVLNALFDLKTVFFLSSPLAPTVSTDAVNMARA
                     TGIPAIFWAAIWIVLALGILWFVMRLYVAGRDKSFQPDLPFEETPPV"
BASE COUNT          545 a          720 c          674 g          496 t
ORIGIN      
        1 catcttcggc acgcgtctgc ctgacacggg ctttacgcaa tacggcggcc tgttgcgcgc
       61 aaacttgaat ctggacgaca acacacaact cctcctcagt tacgcgcgca accagcagga
      121 caacggcaaa cgctacgatc agttgcttgg cggcgacggt aacaacatcg ccgatctccg
      181 caacctcatg accgatactt tttacatccg tctcttccgg cagaagttcg gcatcttcga
      241 caatggctcg tttacttttt cttacaacgg ccagcgggag gagagaatta atcaaggcgg
      301 gcagggcaat ccggctgccg caatcaccaa ccagcgcgag cggaccaatg ttttaggttt
      361 caatttttat ctcgacaaac agatgggtga ccgcaacacg cttcttttcg gtgcggatct
      421 gtatcacgac aagatcaaag ccccggcctt cactttcgat cccgtaacga gaacgaccag
      481 caactcacgt cctcgcattc cgaacggcgc gcgttacgtg tcatccggcg tttacgcgca
      541 aaacaatttc actgcgattc ccgatcgttt acagatcaac ggcgcgatcc gctacagcgt
      601 agcgtcgtac gtttcacgcg ccgcgaactc cgcgcctggg ccaggcggac tgccgttgtt
      661 cccggatgac agcgcgcgct tcgacgcgtt ctccggtcgc atcggcggcg tcgtcaatgt
      721 agccggcggt tttgatatcg ccggaaagta cacacgcgga ttccgtgctc cgaacacgac
      781 cgacctcggt atcgtcgggt tagtcgggac cggtttcgaa gttgacgcca acacggcgtc
      841 agcacgcggc ggttttatcg gcacgacgtc aggcggcgac gcaatttcca ccggcgttcc
      901 ggtgatgtct cttggtccgg agttcagcga cagtttcgat ctcagtttcc gttacacgag
      961 ccagcatttt ggcgctgagc taacaggctt tacgaccaag ctgagcgacg tctacttcga
     1021 tcaggcgctg gtcctgccac ccggggcgac tgggacgttt ctcggcaccg agcagatcat
     1081 cgcgcagaac gaagcagggt tggtgtttgt cgcggctgcg cccaccagtc cagtgctggt
     1141 tcgtgtgaac ttcgatgatg cgcgcatcag cggtatcgag ttcaacacac gtgcgcgctt
     1201 caacgatcaa tttagcggca acggcaactt tacctacgtc cgcgcgcaca gtttattgaa
     1261 cggattgcca ccaagcgtag aagccggcat cccgccggcg acagcatttg tcagtttccg
     1321 ctatcagcca cgcccgcgcg tttatctcga agcgtattcc acactggctg gccggcagaa
     1381 ccggctctcg tcgctcgatc tcgccgatcg gcgcaccggc gccgagcgtt cgcgcaacaa
     1441 cattcagaac ttcttccgcc gtggggcctg tgttcgcggt ctaaccacac ctggaccaac
     1501 cggctgcgga tctgcgggcg gaattctcat tccgaccggc gagacgctgg tgcaagtaca
     1561 ggatcggctg ttgccgattg gcgctgttat caacggcgtg cgtgttgtga acaacgacac
     1621 actggtaccg cttttcccct acttgccggg ctatggtttg gttggtgtaa gaggcgcgct
     1681 gaagtttggt gagacttcag aagtgttcgt cgacttcgaa aacatcctcg acaagtcgta
     1741 ccgcgggatc agttggggca tcgacggcgc cggtcgcggc gtcaccgtcc gctatcgtta
     1801 ttcgttctaa ctagaccggt ggggtttcct caaagggaag gtccggttga aaactcttgt
     1861 cgcggcctgc gacatagaga cgcatcacga accacagaat tcccagagcc aacacaatcc
     1921 aaatagccgc ccagaaaatc gccggaatgc cagtggcgcg ggccatgtta actgcgtctg
     1981 tcgaaactgt cggagcgaga ggtgacgaga ggaagaagac tgtcttcaga tcgaagagtg
     2041 cgttgagcac gcactgaacg gcgagaaaac tgacgaagaa agttgcgacg cgagcagttg
     2101 caaatcgcgc aatcaaaacc aagcccagcg aaaggaacgt tcccgcaagc aatgtgaacg
     2161 gaattccgga ccacgcggtc aaggagaaca tcggcttgag caagccgtag atcatcgtca
     2221 gtccaaagat cagaacagcg gaactcagca agacgatacg ggccgcgatg gctttgcgaa
     2281 tcagcaccaa caacaacgca ccaaacgcca tcgaaccgag ataacccgcg ctggagataa
     2341 agacctgcga aatcagcccg ccttccgttg tgtacgtttc gccgctcgca ttcatcgcga
     2401 cgctgaggct tgcgacagag ttcccggtca ataag
//
LOCUS       DASTEY010000285         6054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1308190, whole genome shotgun sequence.
ACCESSION   DASTEY010000285 DASTEY010000000
VERSION     DASTEY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308190"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFO72_04960"
     CDS             complement(<1..288)
                     /locus_tag="VFO72_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET9478668.1"
                     /translation="MNAHADEHFAGSNKLFISIWVWLVVLTLVEIFLAYKPMSTVIML
                     TILLGLSIIKAALIMAYFMHLKFERLSLILTVVPMLVVCILLLFVFFPDSYR"
     gene            complement(312..995)
                     /locus_tag="VFO72_04965"
     CDS             complement(312..995)
                     /locus_tag="VFO72_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9478669.1"
                     /translation="MSTEARAGHFPAVDWEGGALPYRVGHRKLGMWLFIVSDSLTFSA
                     LLIGYSYLRASNVWPTPFPFYPSIVFASVMTFCLLSSSLTMVFGVSASSKGDKAKARK
                     WILATIFFGAAFVVLHLIEWNHLIHQGMRPLYLPESWVQAFTTTEGVPPSPLFGATFF
                     TITGLHMFHVASGVVYLAVVAARVKKLKHEDVEISGLYWHFVDLVWMFVFPLIYIMSV
                     DVHHLLAAH"
     gene            complement(999..1670)
                     /locus_tag="VFO72_04970"
     CDS             complement(999..1670)
                     /locus_tag="VFO72_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9478670.1"
                     /translation="MVTSTRPPVIKKGTGTGPGVPGPNGNGSKRGGGSFRDPESHANR
                     YRIGMWVGLASVTMMFTSLSSAYIVRSSTANDWISLPMPRVLIASTVLILASSVTLEL
                     ARRKLKASLTKAYTQWVVITVVLGLAFLASQLFAWQQLKAQGLYMSSNPHSSFFYLLT
                     GAHAVHLAGGLLGLTFLWLRSRRITSEAIVLTTRQASADAVAIYWHFMDALWIYLFLL
                     LFLWR"
     gene            complement(1654..3537)
                     /locus_tag="VFO72_04975"
     CDS             complement(1654..3537)
                     /locus_tag="VFO72_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9478671.1"
                     /translation="MSETHEGVHRHPAPTGFIRKYIFSVDHKVIGIQYIMLALAAVIV
                     GMLMSLLMRLNLSWPTTNWPILETLFPTGAPGGIMTPEFYLSLVTMHGTIMVFFVLTT
                     APQGGFGNYFLPIQIGAEDMAFPVLNMLSFWVTLVGFIVILLAIFVEGNQAIGEWFAA
                     FGPSFRGGSGTVGPIGGWTGYAPLSALGRIAGPGQGGGMNLWVISIAIFCVGSLLGAL
                     NFITTLLNMRTKGMSLMRMPLTCWAWFTTAVLALLSFPVLLGGGILLLLDRVAGTSFF
                     IPGGLYVSGLLSGSHPNFPLHTGGSPILWQHLFWFFGHPEVYIAILPGMGATSHILST
                     FARKPVFGYRAMVFAILAIGLLGFFVLVHNMFISGMSPYSSIAFSILTLSIGVPSAVK
                     TFNWLGTLWGSRIRFTTAMLFALGFVSLFVSGGITGLVLGQSSLDLAMHDSYFVTAHF
                     HLVMGVASIFGMFAATYFWFPKMFGRLMSERIGRIHFWFTFIGVYAIFVPFHTMGIRG
                     MPRRFSSFAEYDFLRNVDPLVLFVSIAAILTIAVQFLFYFNVFWSIFKGEKAGDNPWE
                     ATTLEWNTTSPPPHDNFAGVLPTVYHGPYEFAVPGAPKDFVMQTDLEAEVPASNGHNG
                     HKH"
     gene            complement(3596..4444)
                     /locus_tag="VFO72_04980"
     CDS             complement(3596..4444)
                     /locus_tag="VFO72_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9478672.1"
                     /translation="MGRVLAVIIWIITLLSVALFFNRKWWFPEGISQHAPAYDRQFLI
                     TILVVGISFAAAQLALGWAVWKFRDTGKPGDRAIYTHGSNRLEVVWTVITAIVFISLA
                     VMGQSVWASLRLHDAPPGSYTVEAVAQQFQWNFHYAGADNKFGRTDPSLIDDGTLNFV
                     GLDPEDPASRDDAVTAALAIPVNRPVELRLRSKDVIHNFWVPHLRFKQDLVPGMEIKV
                     NFRADRVGKYELACAELCGQLHFKMKSYMLVLPDDELNALQALSQEQFQARMQELLNK
                     YQLPEN"
     gene            complement(4499..5419)
                     /gene="cyoE"
                     /locus_tag="VFO72_04985"
     CDS             complement(4499..5419)
                     /gene="cyoE"
                     /locus_tag="VFO72_04985"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9478673.1"
                     /translation="MEEFAIDLRSPSVAVIERPRLSFRERVSAYVELTKPRITFLIVL
                     TAAAGFALASQGSIDYLAMLSAMFGIGLLSSGIATLNQYAERDLDGLMRRTATRPLPT
                     GKLAPWEALAFGAGITIAAEIYLLVFVNPLTALLGLTVIAGYLFAYTPLKTRTSLSTM
                     VGAFPGAVPPLIGWTAARDAISIEGWVLFAILFLWQFPHFLAIAWMYREDYSRAGILM
                     LPVVEPDGRVTAQQIVVYTLMLLPVSLLPTVLGMSGRIYLAGAALLGLLFLYSSLRAA
                     FSMSRQQARRLLLASVLYLPLLFILMVLNR"
     gene            complement(5427..>6054)
                     /locus_tag="VFO72_04990"
     CDS             complement(5427..>6054)
                     /locus_tag="VFO72_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478674.1"
                     /translation="QGLLGALTVKWMLPLWVSSAHATLAQLFFCTTVGLAVFTSRSWI
                     EARALPEDEKASPLRHMCTIALVLILVQLVLGATLRHSAKWDQHLPTELIVAHIVGAF
                     AVTIALGGTSLSVLRRHKGETFLTRPATIALVLLVVQVFLGIASYVTRLGSPNDPQPL
                     HPMIGVTVAHVACGALVFATTIVLTLRTYRVLPASEAAMEMAARPQES"
BASE COUNT         1474 a         1777 c         1703 g         1100 t
ORIGIN      
        1 ccggtaactg tcagggaaaa acacaaacaa caacaagata cagactacca gcatcggcac
       61 cacagtgaga atcaggctca accgctcaaa cttcaagtgc atgaagtagg ccatgatcag
      121 cgccgccttg atgatcgaca ggcccagcag gattgtcagc atgatgacgg tcgacatggg
      181 cttgtacgcc agaaagattt cgacgagcgt cagcaccacc aaccaaaccc agatcgagat
      241 gaacaacttg ttgctgccgg caaagtgttc gtccgcgtgt gcgttcattt tcgcttccct
      301 ccgtgcgtcg cttaatgagc agccagaaga tggtgcacgt caactgacat gatgtaaatc
      361 agcggaaaaa caaacatcca gacgaggtcg acaaagtgcc agtacaggcc gctgatttcg
      421 acgtcttcat gtttcagctt cttaacccgg gctgctacga ccgccaggta aaccacgccg
      481 ctagcgacgt gaaacatgtg caagccggtg atcgtgaaga aagttgcgcc aaacagcgga
      541 ctcggcggca cgccttcagt cgtggtgaac gcctgcaccc acgactccgg caggtacaac
      601 ggtcgcattc cctggtggat aagatgattc cactcgatca agtggagcac gacgaacgct
      661 gcgccaaaga atatcgtcgc cagaatccat ttccgcgcct tcgctttgtc tcctttgctc
      721 gacgccgaca cgccaaacac catggtcaag ctcgacgaca gcagacagaa cgtcatcacg
      781 gaggcaaaga cgatacttgg gtagaaagga aacggcgtgg gccacacgtt cgacgcgcgt
      841 agataactgt agccgatcag aagcgccgaa aacgtcaacg agtcggagac gatgaagagc
      901 cacatcccca acttgcgatg gccgactcga taaggcaggg cgccgccttc ccaatccact
      961 gccggaaaat gtcctgcacg tgcttcagtg ctcacctgtc acctccaaag gaataggagt
     1021 aggaataggt agatccaaag tgcgtccatg aaatgccagt aaatcgctac tgcgtccgcc
     1081 gaagcctgcc gcgtcgtcag cactatcgcc tcgctcgtaa ttcgtcgcga gcgcagccac
     1141 aggaaagtga gtccgagcaa accgcccgcc aggtgcacgg cgtgcgcgcc ggtgagcagg
     1201 tagaagaaag aactgtgcgg attgctcgac atgtaaagac cctgcgcctt cagttgctgc
     1261 cacgcaaaca gttgcgacgc aagaaaggcc aggccgagca cgacagttat gacaacccac
     1321 tgcgtgtagg ctttcgtcaa cgacgctttc aacttccggc gcgccagctc gagcgtgacg
     1381 ctgctcgcca ggatcaagac cgtgctcgcg atcagtacgc gcggcatcgg caacgaaatc
     1441 cagtcgttcg ctgtcgacga gcgtacgata taggcgctac tcaacgacgt gaacatcatc
     1501 gtcacggacg cgagtcccac ccacatgccg atgcggtaac ggttagcgtg cgactccgga
     1561 tcgcgaaagc tgccgccgcc gcgtttcgat ccgttgccgt ttggtcccgg tactccgggc
     1621 cctgtgccgg tgcctttctt tattaccggc ggtctagtgc ttgtgaccat tgtggccatt
     1681 actcgctggc acctctgctt ctaagtcagt ctgcatcacg aagtctttcg gcgcgccagg
     1741 taccgcaaac tcgtaggggc cgtgatagac agttggtaaa acgcccgcga agttgtcgtg
     1801 tggcggtggc gacgtcgtgt tccactcgag cgtcgtcgct tcccacggat tgtcgccggc
     1861 tttctcgcct ttgaagatgc tccagaagac gttgaagtag aaaaggaact gcaccgcaat
     1921 cgtgaggatc gcggcgatgg aaacgaacag cacgagcggg tcgacgttcc ttagaaagtc
     1981 gtactcagca aagctcgaaa agcgacgcgg catgcccctg attcccatcg tatggaacgg
     2041 aacgaagatc gcgtacacgc cgatgaaagt aaaccagaag tggatgcgcc cgatacgttc
     2101 actcatgaga cggccaaaca tcttcgggaa ccagaagtag gtcgccgcaa acataccgaa
     2161 gatcgacgcg acgcccatca ccaggtggaa gtgcgcggtg acgaaatagg agtcgtgcat
     2221 cgccagatcc agtgagctct gtcccagcac caacccggtg atgccgcccg aaacaaacag
     2281 cgagacgaac cccagcgcaa acagcattgc cgtggtgaaa cgaattctcg atccccacag
     2341 tgtgccgagc cagttgaacg tcttgaccgc ggagggtacg ccgatcgaca acgtcagaat
     2401 cgaaaacgcg attgaggagt acgggctcat gccgctgatg aacatgttgt ggaccaacac
     2461 gaaaaacccg agcaggccga tcgctaagat cgcgaatacc atcgcgcggt acccaaacac
     2521 gggcttgcgt gcgaacgtcg agaggatgtg cgatgtcgct cccatacccg gcaggattgc
     2581 gatgtagacc tcggggtgtc cgaagaacca gaacaggtgc tgccagagaa ttggcgaacc
     2641 gccggtgtgt agcgggaaat tcggatggct gccggaaaga agaccgctga cgtaaagtcc
     2701 gccgggaatg aagaaactgg ttccggcgac gcgatcgagc agcaacagga tgccgccgcc
     2761 gagcaacacc gggaacgaaa gcaatgccag gacagcggtg gtaaaccacg cccagcaagt
     2821 caacggcata cgcatcagcg acatgccctt cgtgcgcatg ttcagaagcg tggtgatgaa
     2881 gttgagcgct ccgagtaacg agccgacgca gaagatcgcg atgctgatca cccagaggtt
     2941 catgccgccg ccctgccccg gaccggcgat ccttccgagg gcgctgagtg gcgcgtatcc
     3001 ggtccagccg cctattggcc caactgtgcc gctcccgcct ctgaatgatg gcccaaacgc
     3061 tgcgaaccat tcgccaatcg cctggttgcc ctcgacgaag atcgccagca ggatcacgat
     3121 gaagcctacg agagtcaccc aaaacgacag catgttcagc accggaaacg ccatgtcttc
     3181 ggcgccgatt tgaatcggca ggaagtagtt gccgaacccg ccctgcggcg cggtggtcag
     3241 cacgaaaaag accatgatcg tgccgtgcat cgtcaccagc gacaagtaaa actcgggagt
     3301 catgatgcca ccgggcgcgc ccgtcggaaa caacgtttcc aggatgggcc aattggtcgt
     3361 cggccaggaa aggttcaagc gcatgagcag cgacatcaac atgcccacta tcaccgccgc
     3421 gagcgccagc atgatgtact gaatcccgat gaccttgtgg tcgacactga agatgtactt
     3481 gcggatgaag cctgtaggcg ctggatgccg gtgtacgccc tcgtgagttt ctgacatctg
     3541 tcgtttctcc tcgttaagct atctcgacaa cttaagagaa cacggattta aaaacttaat
     3601 tttccgggag ttgatacttg ttcagcaact cctgcatgcg cgcttgaaat tgctcctgcg
     3661 acaatgcctg gagcgcattg agttcatcgt ccggcaggac aagcatgtaa ctcttcatct
     3721 tgaagtgcag ctgcccgcac aactcggcgc acgccagttc atacttcccg actctgtccg
     3781 cccggaagtt aactttgatc tccatgcccg gaacgagatc ctgtttaaag cggaggtgcg
     3841 gtacccagaa attgtgaatg acgtctttcg atcgcagccg cagctcgacc gggcggttaa
     3901 cgggaatcgc cagtgccgcg gtgactgcgt cgtctctgct ggccgggtct tcagggtcga
     3961 gaccgacgaa gttcaacgtg ccgtcgtcaa tcagactggg atcggtgcgg ccgaatttgt
     4021 tatcggcgcc ggcgtaatgg aaattccatt gaaactgctg cgccacagct tcgacggtat
     4081 acgagcccgg cggcgcatcg tgcagtcgca gcgaagccca gacgctttga cccattaccg
     4141 ccaggctaat gaagacaatc gccgtgataa cggtccacac cacttcaagc cggttgctgc
     4201 cgtgtgtata gatagcgcga tcgccgggct tgcctgtgtc gcgaaacttc caaacggccc
     4261 agcccagagc gagttgcgcg gccgcgaaag aaatgccgac gaccagtatg gtgatcaaaa
     4321 actggcgatc gtaggcgggc gcgtgttggg aaataccttc gggaaaccac cacttcctgt
     4381 taaagaacag ggccactgat agtagtgtga tgatccagat aataaccgca agaactcgtc
     4441 ccatcggtta cctctcctta gctggtgtct gtcacggcca aactacggcg gcggacaatt
     4501 acctattcag aaccattaag ataaacagaa gcggcaaata gagaaccgat gccaacagca
     4561 acctgcgcgc ttgttgccgt gacattgaga atgccgcgcg cagactgctg tagagaaaga
     4621 gcaatccgag tagcgcggcg ccggctagat agatccggcc cgacatcccg agaactgttg
     4681 gtaacaggct gactggtaag agcatcagcg tgtagaccac gatctgctgc gccgtcacgc
     4741 ggccgtcagg ttcgacgacc ggcaacatca gaattcccgc gcggctgtaa tcttcgcgat
     4801 acatccaggc gatcgccagg aagtgcggaa actgccacag aaacagaatc gcaaacagca
     4861 cccagccttc gatactgatc gcgtcacgcg ccgcggtcca accgatcaac ggcggcacag
     4921 caccgggaaa agccccgacc atcgttgaca gcgatgttct ggtcttcaac ggcgtgtacg
     4981 cgaacaggta gcccgcgatt acggtcaagc cgagaagtgc ggtcaacgga ttcacgaaca
     5041 cgagcaggta gatctcagcg gcaatcgtga ttcccgcgcc aaatgccagc gcctcccacg
     5101 gcgccagttt gccggtgggc agcggccggg tggccgtgcg tcgcatcagg ccgtcgagat
     5161 cgcgttccgc atactggttg agcgtcgcga ttccggaaga caacaggccg atgccgaaca
     5221 tcgcgctgag catcgcgagg tagtcgatgc ttccctgaga agcaagcgca aatccggcgg
     5281 ccgccgtcag cacgatcaaa aacgtgatgc gcggttttgt gagttcgacg taggccgaca
     5341 cgcgttcgcg aaacgacaaa cgaggccgtt cgattacggc gactgacgga ctacgtaaat
     5401 cgatagcaaa ctcttccatt cgtcagctac gactcctgcg gcctcgcagc catttccatc
     5461 gccgcttcgg aggccggaag caccctgtat gttctcaaag tcaaaacgat agtcgttgcg
     5521 aaaaccaacg cgccgcaggc gacgtgcgcc acggtcacgc caatcatcgg atgtaacggt
     5581 tgcggatcgt tcggcgagcc tagtcgcgtc acatacgaag cgatgcccag gaacacttgc
     5641 accacgagca gcaccagcgc gatcgtcgcg ggccgggtaa gaaatgtctc acctttgtgt
     5701 cgtcgcaaca cgctcagcga agtgccgccg agtgctatcg tgaccgcaaa tgcgccgacg
     5761 atgtgcgcca caatcagttc ggttggcaga tgctggtccc atttcgccga gtggcgcaac
     5821 gtggcgccca gaaccaattg caccaagatc aatacgagcg caatcgtgca catgtgccga
     5881 agcgggcttg ccttttcatc ttccggcaac gcacgcgctt caatccagct tcgggaagta
     5941 aaaaccgcca gtcccacggt ggtgcagaaa aagagttgag ccagcgtggc gtgggcactc
     6001 gagacccaaa gcggcaacat ccacttcact gtcaacgcgc cgagtagccc ctgc
//
LOCUS       DASTEY010000286         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_928944, whole genome shotgun sequence.
ACCESSION   DASTEY010000286 DASTEY010000000
VERSION     DASTEY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928944"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFO72_04995"
     CDS             complement(<1..1383)
                     /locus_tag="VFO72_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478675.1"
                     /translation="MTKKLPRREFLQTLGSAAVATAIPVKAQSHPSVAVFREHGFPRI
                     QSADFDIDLPVTFLSEADLIARLNPREFNLFITPYGSAFPKRAWQAMLEYLRGGGNWL
                     NCGGVPLSRPVIRTASGWRAQPYQATYHKRLGITHFFRVATAKIAKYQSGSAALAESF
                     AAQEIYELYVRLSSSNNEPDEAGSDGPHEGVVESLILGLNREGRPIAAPVIQIDRLQA
                     EFAGGRWVFANFTGTLTANAVRVLVEKAARGAVRFRVRSDFACYRAGEVPSLSVETTG
                     VKGNASVEVRDPVGQLVTTDLTRIPRTVRPGLYQISARVGDLTHESGFWVYDAELLRR
                     GKALTVDNHFFYRDGKVFPVTGATYMASDVHRRFLFEPNPFVWDRDFREMKQAGVNMV
                     RTGIWTGWKKYMPEAGKVNERVLRAFDAFLLTAHKYDIPVIFTFFAFLPETWGGSNAY
                     LDPRAVRAQQQ"
     gene            1423..2124
                     /locus_tag="VFO72_05000"
     CDS             1423..2124
                     /locus_tag="VFO72_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9478676.1"
                     /translation="MSIATARQRLLEFLSLSLLIGLTAAIATLVFLGWLTDEVLEGDM
                     RHFDDATRAAVHTLASPGLTTVMRGISFIGSTLFLMLATAAVVIWFALRRWGREAKLF
                     AITMVGASLLNITLKLAFKRERPIPFFDLTPPETYSYPSGHSLASCCFFAGLAAILSG
                     RVKRKRVRSIIWFVATVMFLLIGLSRIYLGVHYTTDVIAGFAAALVWISMVRFVEMQL
                     TRRKKLKSKPQAHLD"
     gene            complement(2098..>2407)
                     /locus_tag="VFO72_05005"
     CDS             complement(2098..>2407)
                     /locus_tag="VFO72_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478677.1"
                     /translation="RNAVRFTPEGTAVEVELRRMNGGGDRFAVITVRDRGKGVPEASL
                     EKIFRPFYRAEDARDRQSGGGTGLGLAITERSVRMHGGSVSATNATDGGLSVEMRLRL
                     "
BASE COUNT          532 a          715 c          641 g          519 t
ORIGIN      
        1 ctgctgctgc gctcttacgg cgcgtggatc gagatacgca ttcgagccgc cccacgtctc
       61 gggaagaaaa gcgaagaacg tgaagattac tggaatgtcg tacttgtgtg ccgtaagcag
      121 aaaagcatcg aacgcgcgca acacgcgttc gtttactttg ccggcctcag gcatgtattt
      181 cttccagccg gtccagatgc ctgtgcggac catgttgaca ccggcctgtt tcatttcgcg
      241 gaagtcgcgg tcccaaacaa aaggatttgg ttcaaagaga aaccgccggt gaacgtccga
      301 agccatgtag gtcgcacccg tgacaggaaa gaccttgccg tcgcgataga agaagtgatt
      361 atcgacggtc aaagccttcc cacgcctgag cagttcagcg tcatacaccc agaacccact
      421 ctcgtgtgtg agatcgccga cgcgcgcgct tatctggtac aacccaggtc ttacagtccg
      481 cggtatccgt gttaagtccg tggtcacgag ctgaccaacc ggatcacgca cttcaacact
      541 ggcattgcct ttcacgccgg ttgtctcgac cgacaaggaa ggcacttcac ccgctcgata
      601 acacgcgaaa tcgctgcgca cgcggaaacg aactgcacct cgcgcagctt tttcgaccag
      661 gactctcacg gcattcgccg tgagagtgcc cgtgaagttt gcaaacaccc aacgcccacc
      721 cgcgaactcc gcctgcaaac ggtcgatctg gatgaccggc gcggcgatcg gccgcccttc
      781 gcgattcagg cccaaaatca gcgactcaac tacaccttcg tgtggcccat cactacccgc
      841 ttcgtcgggt tcgttattag aagaactcaa acgcacgtag agctcgtaaa tctcttgcgc
      901 cgcaaacgac tcagccagcg cggcgctacc actctggtat ttcgcgatct ttgctgtcgc
      961 cacccgaaag aaatgcgtga tcccaagtcg cttgtggtag gtagcttgat aaggctgggc
     1021 acgccaaccc gaagcggtgc gtataaccgg acgcgacaac ggcacaccgc cgcaattcag
     1081 ccagttgccg cccccacgca aatattccag cattgcttgc cacgcgcgtt tcgggaatgc
     1141 agatccgtac ggagtaatga acagattgaa ctcgcgcgga ttgagccgtg cgatcaggtc
     1201 agcctcggaa agaaacgtaa ccgggagatc gatgtcgaaa tccgcgcttt gtatgcgcgg
     1261 aaatccgtgt tcccgaaaca ccgccactga ggggtggctc tgtgctttaa cagggattgc
     1321 tgtagcgaca gccgcgctgc ccaaagtttg cagaaactct cgtcgtggaa gttttttggt
     1381 cacggataga cgcggccata ttacactagc tcgcgagtac agatgtcgat cgcgacagcc
     1441 agacagcgcc tcctcgaatt tctcagtctc tcgttgctga tcggattaac ggcagcgatt
     1501 gcgaccctcg tctttctcgg ctggcttacc gacgaggtcc tcgaaggcga catgcggcac
     1561 ttcgacgacg cgacgcgtgc ggccgttcac acactcgcgt cgccaggatt gacgaccgtg
     1621 atgcgcggca tctcgtttat cggttcgact ctgtttctca tgctagcgac agctgctgtt
     1681 gtgatctggt tcgcactgcg tcgttggggc cgcgaagcaa aactcttcgc aatcacgatg
     1741 gtcggcgcgt cgttgttgaa tatcacgctg aagttggcgt tcaaacggga acgcccgatc
     1801 ccatttttcg acctgacgcc tccggaaacg tacagctatc ccagcggtca ctcgttagca
     1861 agttgctgct tcttcgccgg attggccgca atcctctccg gtcgagtgaa aagaaaacgc
     1921 gtgcggtcca tcatttggtt tgtggccaca gtgatgtttc ttttgatagg gctgtcacgg
     1981 atttatctag gcgtgcatta caccacggat gtaattgcgg gatttgcagc agccctggtc
     2041 tggatatcaa tggtgagatt tgtggagatg caattaacga gacgcaaaaa actcaaatca
     2101 aagcctcagg cgcatctcga ctgagagtcc gccatccgtg gcattcgtgg ctgagaccga
     2161 tccgccatgc atgcgcaccg agcgttcggt gattgcaagg cccaaaccag tgccaccgcc
     2221 actctgccga tcgcgcgcgt cttccgcgcg ataaaacgga cggaaaatct tttcgagcga
     2281 cgcttcagga acacctttgc cgcgatcgcg aacagtgatc accgcaaacc ggtctccacc
     2341 accattcatc cgccgcagtt caacttccac tgctgtgcct tcaggcgtga agcgaaccgc
     2401 atttcgt
//
LOCUS       DASTEY010000287         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_401648, whole genome shotgun sequence.
ACCESSION   DASTEY010000287 DASTEY010000000
VERSION     DASTEY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401648"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2060)
                     /locus_tag="VFO72_05010"
     CDS             complement(<1..>2060)
                     /locus_tag="VFO72_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478678.1"
                     /translation="LNTPDADAAHQAFSNLFVETEFFAGENAILAHRRVRSSEDKPIW
                     GVHVVVAEGDLMGAVQYETDRGRFLGRGRTPSNPIAVMEDRPLSNTTGAVLDPVFSLR
                     RRVRIPPNQTVRFAFSTAVAHSREEAIALADKYHDPNIFERELRLAWTKAQVEMSHLK
                     MDAEEAHLFQRLAARIVYSDPSLRPRQHVLALNTKAQSSLWTYGISGDLPIVVVRISN
                     ADDLRTVRKLVRGHEYLHYKGLNIDLAILNDTPTSYLQLLHQELETIVRTSGLQGLQD
                     KPGGVYLRRADQMPEADRILLHAVARVVIVAERGSFEDQIERPRVQEPLPPPLVPRLP
                     SQTYPELTVPPPELTFFNGLGGFHQGGREYVTLLGAEQWTPAPWSNVIGNSVDFGFQI
                     TETGGGCTWSLNSRENRITPWSNDAVSDPPGEVVYLRDEDTGTVWSVTPLPVREREPY
                     IIRHGQGYSVFEHTSHGISQELLVFAPLDAPVKVSLLRLRNRTGRKRRLSVTNYNELV
                     LGVSRSSSAPYIITEIDQDASTIFAKNPFNNEFAERVAFVASSEGFSSATCDRKEFIG
                     RNSSLETPAALRRAGLAGRDGAGLDPCAAIQTTIELAPNEAREVFFLLGEEASKTAAE
                     GVITKFRQAGTVNAAFEKVLTHWDELLGTIEVRTPDTALDTMLNRWLLYQTLSCRVWA
                     RTAF"
BASE COUNT          408 a          604 c          618 g          430 t
ORIGIN      
        1 gaacgcagtg cgcgcccaga cgcgacacga aagcgtctgg tacagcagcc agcgattgag
       61 catcgtgtcc aacgctgtgt ccggcgttcg cacttcgatc gtgccgagca gttcgtccca
      121 gtgggtcagc actttttcga acgccgcgtt gactgttccc gcttgcctga acttagtgat
      181 aacaccttcc gctgcagttt ttgacgcttc ctcgcccagc aggaagaaaa cttcacgagc
      241 ttcgttcggc gccagctcga tggttgtttg aatcgccgcg caaggatcca agcctgcccc
      301 atcgcgtcct gccagtcctg cacgcctgag agcggcgggc gtttccagtg agctgttgcg
      361 accgatgaac tccttgcgat cgcacgtcgc actggagaat ccttcacttg aagcaacaaa
      421 agcgacgcgc tcggcaaatt cgttattgaa tgggttcttc gcaaagatcg tggacgcgtc
      481 ttgatcgatc tcggtgatga tgtaaggcgc cgatgacgat cgcgaaacgc cgagcaccag
      541 ctcgttgtag ttggtcaccg acagtcgacg tttccggccc gtgcgattgc gcaaacgcag
      601 cagcgagacc ttcaccggcg catcgagcgg tgcaaagacg agcaactcct gggaaatgcc
      661 gtggctggtg tgttcgaaaa cgctgtagcc ctggccgtgc ctgatgatgt acggctctcg
      721 ctcgcgaacc ggcaaaggcg taacggacca gacggtgccg gtgtcttcgt cgcgcaggta
      781 gaccacttcg cccggcggat cgctgaccgc atcgttggac cacggcgtga tacggttctc
      841 gcggctgttg agcgaccacg tacacccgcc gcccgtttcc gtgatctgaa atccgaaatc
      901 aacagaattg ccaatgacgt tggaccaggg agccggcgtc cactgttccg cgcctaacaa
      961 cgtcacgtac tcgcggccac cctgatgaaa accacccagc ccgttgaaga aagtcagttc
     1021 aggcggcggc accgtcagct caggatacgt ttgggaaggc agccgtggca caagaggagg
     1081 tggcaaaggt tcctggacgc gcggtcgctc gatctgatcc tcgaacgagc ctcgctcggc
     1141 aacgatgaca acgcgggcca cggcgtgtag caaaatgcga tctgcttcgg gcatctgatc
     1201 tgcccgtcgc aggtaaacac cgccgggttt gtcctgcagc ccttgcaggc cgctggtgcg
     1261 cacgatcgtt tccagctcct ggtgcagcaa ctgaagatag ctggttggtg tatcgttcag
     1321 aattgcgaga tcgatattga gtcctttgta gtgcaggtac tcgtgcccgc gcactagctt
     1381 ccgcaccgtt cgcagatcgt cggcgttgct gatcctgacg acgacgatcg gaagatcgcc
     1441 actgatgccg taagtccaca ggctcgattg tgctttggtg ttcagcgcga gcacgtgctg
     1501 tcgcgggcgt aatgacggat cggaatagac gatgcgcgcc gcgagacgtt ggaagagatg
     1561 cgcctcttca gcgtccatct tgaggtgact catctcgact tgcgctttgg tccatgccag
     1621 gcgcagctcg cgctcgaaaa tattcggatc gtgatatttg tcagcgagcg ctatcgcttc
     1681 ttcgcgcgaa tgtgccacgg ccgtgctgaa cgcaaatcgg accgtttggt tgggcggaat
     1741 tctcacgcgt cttcgcaggc tgaaaaccgg atcgagcacg gcgccagttg tgttggacag
     1801 cgggcgatct tccatcaccg caatcggatt actcggcgtt cgcccgcgcc cgaggaagcg
     1861 gccgcgatcg gtttcatatt gcaccgcgcc catcaaatca ccttcggcaa cgacgacgtg
     1921 aacgccccag attggtttgt cttcggaaga acgaacccgg cggtgcgcga ggatggcgtt
     1981 ctcaccggcg aagaattccg tctcgacgaa gagattgctg aacgcctggt gggcggcatc
     2041 agcgtcgggt gtgttgagca
//
LOCUS       DASTEY010000288         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1308233, whole genome shotgun sequence.
ACCESSION   DASTEY010000288 DASTEY010000000
VERSION     DASTEY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308233"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..921)
                     /gene="larB"
                     /locus_tag="VFO72_05015"
     CDS             complement(157..921)
                     /gene="larB"
                     /locus_tag="VFO72_05015"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET9478679.1"
                     /translation="MDLKELARLLEAHKHGELESSEAIERIKNLHFEDIGFARVDHSR
                     ATRLGFPEVVFGGGKTRAQVVGIVERLAQRSPNVLVTHTDEGTFGEVRNVVTDAEWHE
                     APRLIRIRRDKRDHGVGPIAVITAGTSDIPVAEEAALTAETMGNTVSRIWDAGVAGIH
                     RVLAERSLLQRSRVVIVAAGMEGALPSVVGGLVGVPVVAVPTSIGYGASFGGVAALLG
                     MLNSCASNVTVVNIDNGFGAGFVASLINRRWEPPTE"
     gene            1046..1912
                     /locus_tag="VFO72_05020"
     CDS             1046..1912
                     /locus_tag="VFO72_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HET9478680.1"
                     /translation="MSTVIKNTRLTLEMIKIEHTLFALPFAFLGAVLAADGLPTLRQL
                     FWIVVAMVGARSAAMAFNRIADRNIDARNPRTATRALPAGLLSVGFVLAFTIVSAAVF
                     LLAAAMLNRLTLLLAPVALASVLLYSFTKRWTQFSHLVLGWCLSIAPTGAWIAVRGEI
                     GSAVPLLLSLVVLLWTAGFDILYACQDFDFDRREGLRSIPARVGIGRALWIARSLHAA
                     AFLSLVWLYALTGLGPLAFAGVIATGALLVYQHRLVRADDLGRLNAAFFTTNAFVSVI
                     LLVTFGSAVFLN"
     gene            1938..3062
                     /gene="mqnE"
                     /locus_tag="VFO72_05025"
     CDS             1938..3062
                     /gene="mqnE"
                     /locus_tag="VFO72_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HET9478681.1"
                     /translation="MTFSHDDKLNDIAARAFAGERLSFADGVSLYETDDLPALGKLAD
                     TIRRRRHGRTTYFNVNRHLNPTNICYADCKFCGFFRTPRQPDAYTHNIDDSLKVAAQA
                     VAEGATELHITGGLNTKLPFSYFTDLLSSLKREYPQLHLKAFTMVEFDHFAHFYKMPD
                     DEVIQKLIDAGLDSCPGGGAEIFREPTRSMICAHKCDADRWLELQGKVHAAGLKTNAT
                     MLYGHIESIEDRVDHLIRLREQQEKSGGFQCFIPLAFYPPETQLSHLPGPSGVDSLKT
                     MAVSRLLLDNFYHIKAYWVMLGKRLAQVALHYGANDIDGTITEGGELTTSYSVEANNE
                     VRMTKPELIGLIEDAGFEAVERDTVYNRVDRSNVSLSEAV"
     gene            3178..4032
                     /locus_tag="VFO72_05030"
     CDS             3178..4032
                     /locus_tag="VFO72_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HET9478682.1"
                     /translation="MITDTESTTITLAHSPDSDDAFMFYGLATNKIDTGDLHFEHLLK
                     DIQTLNEEAMRGTYDVTAISFHAYAYIADKYALLPHGASIGDNYGPVVVSRKPATQDD
                     IPHLKIAIPGKLTSAFLALRIFNSEFEYEVVPFDKIIDAVQKGECDAGLLIHEGQLFY
                     ESKGLHKVLDLGEWWHSRHGLPLPMGGNAIRRELGTDLVTKISLLLRESIQYSLTHRE
                     DALQYAMQFARDMEPDLADRFVAMWVNDLTLDYTERGREAVRLLLQEGFEKGIIPHRV
                     PIDFVSED"
BASE COUNT          917 a         1218 c         1138 g          899 t
ORIGIN      
        1 agcagtgaag acggaggaaa ccgtttcaac ggttttccgt tgattcgcca gcgaaaccgt
       61 taaaacggtt tctcaaaacc gcgtggcggt aacaccgggc tgaagcccgg tgagaatgaa
      121 agaagacttt tgaggcaaag cctcggcaga ccttgattac tcagttggtg gttcccagcg
      181 ccggttgatc aaactggcga cgaagccggc gccaaagccg ttgtcgatgt tgaccactgt
      241 gacgttcgag gcgcacgagt tcaacatgcc cagcagcgcc gcgacaccac caaaagatgc
      301 gccgtaaccg atactggttg gcacagccac gacgggcaca cctaccaaac caccaaccac
      361 cgagggcaac gcgccttcca ttcccgccgc cacaatcaca acgcgcgaac gttgcaacaa
      421 gctccgctcc gcgagaacgc gatgaattcc ggcaacgcct gcatcccaga tgcgcgacac
      481 cgtgttgccc atcgtttccg ctgtcaaagc tgcttcctcg gcgacgggaa tgtcgctcgt
      541 cccggcagta atcacggcaa ttggaccaac accgtgatcc cgcttgtcac ggcgaattcg
      601 gatcaggcgc ggggcttcgt gccactctgc gtccgtgacc acattacgca cttcgccgaa
      661 agtaccttca tcggtgtgcg tgacgagaac gtttggcgaa cgttgcgcga gccgttcaac
      721 aatgccgacg acttgtgcgc gtgtttttcc gccgccaaaa actacctctg gaaaacccag
      781 ccgcgtagca cgggagtggt cgacacgagc gaacccgatg tcctcaaaat ggaggttctt
      841 gatacgctcg atcgcctcgc tgctttccag ttcgccatgc ttgtgcgctt ccagcagtcg
      901 tgccagttcc tttagatcca tgcttgtcgc accagggctg cattctaaca ttgaacttcc
      961 gcaacgccct ccgcgtttgc gataacagca agagcgcaag taagattcag gcaaatgccc
     1021 ggttcaaacg aagattagac ccaccgtgtc gacagtcatc aaaaacaccc gtttgacgct
     1081 cgagatgatc aagatcgagc acacgctatt tgcgctgccg tttgccttcc tcggcgcggt
     1141 gctggctgcc gacggcttgc ccacactacg acaacttttc tggatcgtag tcgcaatggt
     1201 gggcgcgcga tccgcggcga tggctttcaa ccgcattgca gacagaaaca tcgacgcccg
     1261 caacccgcgc acagcaacgc gcgcattgcc cgccggcttg ctgtcagtcg gtttcgtctt
     1321 ggcgtttaca attgtttccg cggcagtttt cctgttggca gccgcgatgc taaaccgact
     1381 cacgctgctg ctggcgcccg tggccctcgc aagcgtactg ctttactctt tcacaaaacg
     1441 ctggacgcag ttttcgcacc tggtgctggg ttggtgtctg tcaatcgctc caaccggcgc
     1501 atggatcgcg gtgcgtggcg agattggaag cgcggtgccg ttgctgttgt cactcgtggt
     1561 cctgctttgg accgccgggt tcgatattct ttacgcctgc caggatttcg atttcgaccg
     1621 ccgagaaggt ttgcgatcga ttccagcgcg tgtcgggatc gggcgagcgc tgtggattgc
     1681 gcgctcgctt cacgccgctg cttttctttc gctggtgtgg ctgtatgcgt taaccggtct
     1741 tggaccattg gcgtttgccg gagtgatcgc gactggcgct ttgctggtct accaacatcg
     1801 tctcgtccgc gcagacgatc tcggaaggtt gaatgccgca ttctttacga ccaacgcctt
     1861 tgttagcgtg attctgctgg taacgttcgg tagcgccgtg ttcctgaatt gacaggccga
     1921 cgatagaaag tatggaaatg actttttcac acgacgacaa actcaacgac atagccgctc
     1981 gcgctttcgc gggagagcga ctctccttcg ccgacggcgt ttccctgtat gaaacagacg
     2041 atctgcctgc actgggcaaa cttgccgaca ccatcagacg tcgccgtcac ggccgcacga
     2101 cctatttcaa cgtcaacagg cacctgaatc cgaccaacat ttgctacgcc gattgcaagt
     2161 tctgcggctt tttccgcacg ccacgccagc cagacgctta cacacacaac atcgacgaca
     2221 gtctgaaagt cgccgcccag gccgtcgctg aaggtgcgac cgaactgcac atcactggtg
     2281 ggctgaacac aaaacttcca ttcagttatt tcactgacct gctgtcttcg ctaaagcgcg
     2341 agtatccgca actgcacttg aaagcattta caatggtcga gttcgatcac ttcgctcact
     2401 tttacaagat gccggacgac gaagtgattc aaaaactgat cgatgctggc ttggattcat
     2461 gtccgggcgg cggggcggag atctttcgtg aaccgacgcg ctcgatgatc tgcgcacaca
     2521 agtgcgatgc cgatcgctgg ctcgaactcc agggaaaagt tcacgcggca ggtttgaaga
     2581 cgaatgcgac gatgctctac ggccacatcg aatcgatcga agatcgggtg gaccatctga
     2641 ttcgcttgcg cgagcaacag gaaaaatccg gtggttttca atgctttatc ccgctggctt
     2701 tttatccgcc cgagacgcag ttgtcgcacc tgcccgggcc gtccggcgtc gattcgttga
     2761 aaacgatggc ggtgtcgcgc ctgctactcg ataacttcta ccacatcaaa gcttactggg
     2821 tgatgctcgg gaaacggctt gcacaagtgg cgctgcacta cggcgccaac gatatcgacg
     2881 gcactatcac cgaaggcggc gaattgacga ccagttattc agtagaagcc aacaacgaag
     2941 tcaggatgac caagccggaa ctcatcggtc tcatagagga cgcgggcttc gaggcagtcg
     3001 aacgagatac cgtttacaac cgcgtcgacc gtagtaatgt ttcgcttagt gaggccgttt
     3061 aacctgggac cgcaggcgtc tcgcctgcga agtggcgaat cgagacgcag ccctgaattg
     3121 caggcgagac gcctgcggtc ccaggtttac agcatctaaa ataccgttta ctcaagaatg
     3181 attaccgata ccgaaagcac caccatcacc ttggcgcact cgcctgattc cgacgacgcc
     3241 ttcatgttct acggcctcgc caccaacaag atcgacaccg gcgacctgca cttcgaacat
     3301 ctattgaaag atatccagac gctcaacgaa gaagcgatgc gcggcacgta cgatgtcacc
     3361 gcgatcagct ttcacgcata tgcgtacatc gccgacaagt acgcgctgct gccgcacggc
     3421 gcttcgattg gcgataatta cggtccggtt gtggtttcgc gcaagcccgc aacacaggac
     3481 gatatcccgc atttgaagat tgccattccc ggcaagctga cgagtgcgtt tctggcgctg
     3541 cggatattca attccgagtt cgagtacgaa gtggtgccgt tcgacaaaat catcgacgca
     3601 gtacagaagg gcgagtgcga cgccgggctt ctgattcacg agggacagtt gttctacgag
     3661 tcgaagggcc tgcacaaagt gctggatctc ggcgagtggt ggcacagccg gcacgggttg
     3721 ccgttgccga tgggcggaaa cgccattcgg cgcgagctgg gaacggattt agtaacaaaa
     3781 atctcgctct tgttgcggga gagcattcaa tattccctga cgcaccgcga ggacgcgctt
     3841 cagtacgcca tgcagtttgc ccgcgacatg gagcccgatt tggcggacag atttgtagca
     3901 atgtgggtca acgacctgac tctggattac acagagcgcg ggcgagaagc tgttcgccta
     3961 ttgttgcagg aggggtttga gaaagggatc attccccacc gcgtgcctat cgattttgta
     4021 tctgaagact gagatttaga gacgaaaagc tggggtgtgc gcggcggttt tttaagctac
     4081 cccgcccaga aggggcagct aaccgccgcg cacatcatcg acatcagacc acgtaaaacc
     4141 aagctcctcc caaagcctgg tttatcgtcc cc
//
LOCUS       DASTEY010000289         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1538799, whole genome shotgun sequence.
ACCESSION   DASTEY010000289 DASTEY010000000
VERSION     DASTEY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538799"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..1584)
                     /locus_tag="VFO72_05035"
     CDS             complement(358..1584)
                     /locus_tag="VFO72_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9478683.1"
                     /translation="MLQPPNRPLNIICLATYFKGVDFIRECKAQGCHVVLITKKKMLN
                     EDWPRESLDDLIAVPNDAGPALFIDLLAFLARNRPVHRVIALEEFDVVTAALMREHLC
                     LHGLSSSRAKIFRDKLSMAVYSQRAGINVPDFVQLVNHDEINDFMQRVPGPWVIKPRS
                     DVSAIGIKKVEHPNDVWGAIEEMNARQNLRERASYYLLARFIPGKVFHVDSLVNDGKV
                     VFAGANQYGRPPIQVAHQGGAYISRTVARGSADEKALLQVNRKLIKALRLQCGASHAE
                     FIRSDADGKFYFLEIAARVGGAYIAEVLEAASGVNLWREWARFEVADEKAPAKISPPR
                     KEYAGISLSLAKQEHPDTSAYVDEEIVYRVKKRHHAGLIVRAQQLERVTELLDSYAQR
                     FADDFVAVAPPPERPE"
     gene            1714..>2537
                     /locus_tag="VFO72_05040"
     CDS             1714..>2537
                     /locus_tag="VFO72_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9478684.1"
                     /translation="MIFKGPYPDVHIPETALTPFVMHRAAELADKAALIDGPTGRTLT
                     YAQFADAIAIVAHNLAERGFKKGEVIGILSPNCPEYGIAFHAVATLGGVVTPINPLYT
                     RHEIAHQLKDAGARFLIATTMCIDRASEAAEDAGVEEIFVFGHTDRATPFAELLVDNG
                     RATQVDINPREDLVALPYSSGTTGLPKGVMLTHHNLVANLCQMEGLDYFYADDTLICV
                     LPLFHIYGLVVVLNMGLFAGATVVTMPRFDLESFLKAVQDYDVTLAHIVPPIVLAL"
BASE COUNT          522 a          739 c          723 g          553 t
ORIGIN      
        1 ggacgaacac ctgtatcaca ctaaaggttg ggcgcgtgaa ctgtggatcg agtcgttggg
       61 aatgaaggcc gtgctgccgc cacctgaaga ggtcaaacag gtggaaacgg caatcggtgc
      121 ggcgcgtgct gaacaggcgc gtacaaggat gatgtagagt tggaccacga accgcggtct
      181 tgctggggcc gcaggcgtgt tgcctgcaag tgaacagcaa agggcgccgt tcgtatcggg
      241 caggcgagac gcctgcggtc ccagaatcgt actgccggcg ggtccaaaaa tacgtccggg
      301 gggacggata gaggcgagta aggtttgtga aaccgctcgc ctcttttcgt atctcgctta
      361 ctcgggtctt tccggtggcg gagcgacggc gacgaaatcg tcagcaaagc gttgcgcgta
      421 gctgtcgagc aactccgtca ctcgttccaa ttgttgcgca cgcacgatta gtcccgcgtg
      481 atggcgtttc ttgacgcggt agacgatctc ttcatcaacg taggctgacg tatccggatg
      541 ttcctgtttg gccaacgaga gacttatccc tgcgtattct ttgcgcggcg gcgagatctt
      601 cgccggcgct ttttcgtccg cgacctcgaa cctggcccat tcacgccata aattcacacc
      661 cgacgctgct tcgagtacct ccgcgatgta cgcgccgcca acgcgcgctg cgatctcgag
      721 gaaataaaac tttccgtcag catcgcttct gatgaattca gcgtgactgg caccgcattg
      781 cagacgaagt gccttgatca acttcctgtt gacctgcagc agcgccttct catcagccga
      841 accgcgggcg actgtgcgcg agatgtaggc gccgccttga tgggccacct gtatcggcgg
      901 acgtccatat tgattcgctc ccgcaaacac gaccttgcca tcgttcacca gcgaatcgac
      961 gtgaaacact ttgccgggaa tgaatcgcgc gagcaggtag taagacgcgc gctcgcgcag
     1021 gttctggcgc gcgttcatct cctcgattgc tccccatacg tcgttcgggt gctcaacctt
     1081 cttgatgcct attgccgaga catccgaacg cggtttgatc acccacggtc cgggaacgcg
     1141 ttgcatgaag tcgttgattt cgtcgtgatt cacgagttgc acaaaatccg gcacgttgat
     1201 gccggcgcgc tggctgtaca cggccatcga cagcttgtcg cgaaagatct tcgcgcgcga
     1261 gctgctgaga ccgtgcagac agagatgctc gcgcatcagt gcagcggtaa caacgtcgaa
     1321 ctcttcgagt gcgataacgc ggtggacagg acgattccgg gcgagaaatg cgagcagatc
     1381 tatgaacaag gctgggcccg cgtcgttggg aacggcgatc aggtcgtcga gactctcgcg
     1441 cggccagtcc tcattgagca tcttcttctt ggtgatcaat acgacgtgac aaccttgcgc
     1501 cttgcattcg cggatgaagt caacgccctt aaaataggtg gccagacaga tgatgttgag
     1561 agggcgattt ggaggctgga gcatttcgcc cactataaac cagcttggct tgtctatcca
     1621 cagattacgc ggattgtttt ccgcttttca ggtttccgct cacctgttaa ggtatcggac
     1681 gcaatcgaac ccgtactctt ccaacaaaca acgatgatat tcaaaggtcc ctatccggac
     1741 gtacatattc ctgagactgc gctaacaccg ttcgtcatgc accgcgccgc cgaactggcc
     1801 gataaagcgg ccctgatcga tggaccgacc ggccgcacgc tgacttatgc gcagtttgct
     1861 gacgcgatcg ccatcgtcgc ccacaatctc gccgagcgtg gtttcaaaaa gggcgaagtg
     1921 atcgggattt tgagcccaaa ctgtcccgaa tacggcatcg cctttcacgc cgttgcgacg
     1981 ctcggcggag tcgtgacgcc catcaatccg ctgtacacgc ggcacgagat tgcacaccaa
     2041 ctaaaagacg cgggcgcacg ctttttgatc gccacaacga tgtgtatcga tcgcgccagc
     2101 gaagctgctg aggacgcggg cgtcgaagag atttttgttt tcggccacac tgaccgcgcg
     2161 acaccgtttg ccgagttgct ggtcgacaac ggccgcgcta cgcaagtgga tatcaatccg
     2221 cgcgaagacc tggttgcgct tccctactcc agcggcacta cgggcttgcc caaaggcgtg
     2281 atgctcacgc accacaatct ggtcgcgaac ctctgccaga tggaaggtct cgactacttc
     2341 tacgcggacg acacattgat ctgcgttctg ccgctctttc acatctacgg cctggtagtg
     2401 gtgctcaaca tgggactgtt tgcgggcgcc accgtcgtca cgatgccgcg ttttgatctg
     2461 gaatcgtttc tgaaagccgt tcaggattac gacgtcacgc tcgcgcacat cgtgccgccg
     2521 atcgtgctgg ccctctg
//
LOCUS       DASTEY010000290         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_6250, whole genome shotgun sequence.
ACCESSION   DASTEY010000290 DASTEY010000000
VERSION     DASTEY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6250"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1151)
                     /locus_tag="VFO72_05045"
     CDS             complement(180..1151)
                     /locus_tag="VFO72_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9478685.1"
                     /translation="MRNTPEPHHDYSSAHHHFHGSGRSSDNWRRLSVVLSLTALYMIA
                     EAIGGWWTGSLALLADAGHMFTDVAALALALTAVWFGSRPATSKKTFGYYRLEIIAAL
                     VNGVTLVVISLFIFYGAYERWLEPQFVRAEPMMAVAAGGLAVNLICAWILHRRNEVDL
                     NIRGAWMHVMGDALGSVAAILAGVFMSLFGWFAADAVFSAFIGVLIIWGSMRLIREST
                     NVLLEGTPAHINLAAVEDEILATNGVSDVHDLHVWTITSGRDALSAHVIHSSNIPQAV
                     LLRELRTKLHDRFGVDHLTIQMETSDFEDETFHFCHAGTACFRSERN"
     gene            complement(1184..1948)
                     /locus_tag="VFO72_05050"
     CDS             complement(1184..1948)
                     /locus_tag="VFO72_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET9478686.1"
                     /translation="MIIVCPKCSTRLQVDQEKSTNRPFNVRCPKCNSTISSGGASPAL
                     EQSALAVGGSPATEHPRFEQPTARVYEPLTKTAQPVDAATPSEALQMLADLLSKSAGG
                     ETKKPGARPAWDKRKALLCASEPYRDTVARKLTDSGYQVFVAEETRQAVETMRSKQLD
                     VVLLDPQFDPAEQGSAFVVREVNVLRPAQRRRLFFVLLSPSMRTMDAHAAFLNNVNAI
                     VNINDLDELDRVMGIALREYNELYREFYAAFGLPAL"
     gene            complement(1957..>2668)
                     /locus_tag="VFO72_05055"
     CDS             complement(1957..>2668)
                     /locus_tag="VFO72_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9478687.1"
                     /translation="INEKKPYHIVTIEDPIEYLHLHKRSTINQREVGHDTRDFPSALR
                     ATLRQAPKVILIGEMRDFETTEIALEAAETGHLVLSTLHTIDASKTVDRIIGLYPKNE
                     EPVIRTRLAQTFRYIVSQRLIPKADGSGRIAAVEILRSSPRTREYIETGEVEGKSLLD
                     AMRDGKLDGMQDFDTVIKGLIESKIVSFEDGLAFATNQNNLLLSLKGMTAAEDFIRQD
                     QMPANSFSDSGSMLGMIE"
BASE COUNT          600 a          785 c          733 g          550 t
ORIGIN      
        1 gcgcttccgc gagccactta cgcaactgtc gcgcttcgtc cattgaagcg atgcgacaag
       61 cgcccgtaag cgccagcgta accagcgcgt ccggggcacc gcgagttacc aatgcggcct
      121 gcaatgccgc ctgttcccgc agatcgagcg tgattagaag actcagttcc atccgtggct
      181 caatttcttt ccgaacgaaa acacgccgta cccgcgtgac aaaagtgaaa tgtctcgtct
      241 tcgaaatccg acgtctccat ctgaatcgtc agatggtcga cgccgaaccg atcgtgcagt
      301 ttcgttcgca actcccttaa cagaaccgcc tgaggaatgt tgcttgaatg aatcacgtgc
      361 gcactcaatg catcgcgtcc ggaagtgatg gtccaaacat gcaagtcgtg aacatcagac
      421 acgccgtttg ttgcgagaat ctcatcttcc accgcagcga gattgatgtg tgccggcgtg
      481 ccttcaagca gcacgttggt tgactcgcga atgagcctca tggaccccca aatgatcagc
      541 acgccaatga aggcactgaa aaccgcgtcg gcggcgaacc aaccaaacag cgacatgaac
      601 acgcccgcga gaatcgcagc caccgaaccg agcgcgtcgc ccatcacgtg catccaggcg
      661 ccgcgaatgt tgagatctac ctcgttgcgc cggtgcagta tccacgcgca gatcaaattc
      721 acggcgagac cgcccgcggc aaccgccatc atcggctcag cgcgcacaaa ctgcggctcg
      781 agccagcgtt cgtaagcccc gtaaaagatg aaaagcgaaa taacgacgag cgtgacgcca
      841 tttaccaagg ccgcgattat ttccaaacgg taataaccaa aggtcttttt cgaagttgcc
      901 ggacgagatc caaaccaaac cgcagttaac gccagagcca gcgcggcgac gtccgtgaac
      961 atatgacccg catcagcgag caacgccagc gatccggtcc accatccgcc gatcgcctcc
     1021 gcaatcatgt agagtgcggt cagcgagagc accacactca aacgtcgcca gttgtcagac
     1081 gaacggccag atccgtggaa gtgatgatgc gcagaactat agtcgtgatg gggctccggc
     1141 gtgtttctca tcgccacaaa ataatgccag tgacgaccgc gaactacaaa gccggaagac
     1201 cgaaggcggc gtagaactcg cggtagagtt cgttgtactc gcggagagcg atacccatga
     1261 cgcgatccag ttcgtcgagg tcgtttatgt tgacgatcgc attcacgttg ttgagaaaag
     1321 ctgcgtgcgc atccatcgtg cgcattgatg gcgacaacag aacgaagaat aagcgacggc
     1381 gttgagccgg ccgcaagaca ttcacctctc tcacgacaaa cgcggaacct tgctcagccg
     1441 gatcgaactg cggatcaagc aaaaccacgt ccaactgttt cgaacgcatt gtctcgaccg
     1501 cctggcgcgt ttcctcggca acgaaaacct ggtagccact gtccgtgagt tttcgcgcca
     1561 ccgtatcgcg ataaggttcg gacgcacaga gcaatgcttt gcgtttgtcc cacgccgggc
     1621 gggcgcccgg tttcttagtt tctccgcccg cgctcttgga aagcaggtcc gcaagcattt
     1681 gcagagcttc gctgggcgtc gccgcatcaa ccggctgcgc cgttttagtc aacggttcgt
     1741 aaacgcgtgc ggtcggttgt tcaaaacgcg ggtgttcggt ggctggcgag ccaccaactg
     1801 ccagcgcact ctgctccagc gccggactcg cgccacctga actgatcgtg ctgttgcact
     1861 tcggacagcg aacattgaac ggacgattgg tcgacttctc ctggtccacc tgaagacgcg
     1921 tcgaacactt gggacagaca atgatcatgg gctttgttat tcgatcatgc cgagcatgga
     1981 accggagtcg gaaaaagagt tggccggcat ctgatcctgc cgaatgaagt cttcggcggc
     2041 ggtcatgccc ttgagtgaca ggagcaggtt gttttggttg gtcgcaaacg cgagcccgtc
     2101 ttcgaacgaa acgattttcg attcgatgag ccccttgatc acggtgtcga agtcctgcat
     2161 tccgtcgagc ttgccgtctc gcatcgcatc gagcaacgac ttgccctcaa cttcacccgt
     2221 ttcgatgtac tcacgcgtgc gcgggctcga acgcagaatc tctaccgctg caattcgtcc
     2281 cgagccgtca gctttgggaa tcagtcgctg cgagacgata tagcgaaatg tttgggccaa
     2341 ccgcgttcgg atcaccggct cttcgttctt tggatacaag ccgatgatgc gatcgacagt
     2401 tttcgaagca tcgatcgtgt gcagagttga aagcacgagg tgcccggttt cggccgcctc
     2461 caacgcgatc tccgtcgttt cgaagtctcg catttcgcct atcagaatca cctttggcgc
     2521 ctggcgcagc gtcgcgcgca gggccgaggg aaagtcgcga gtgtcgtgac caacctcgcg
     2581 ttgattgatg gtcgaacgtt tgtgcagatg gagatactcg attgggtctt cgatcgtgac
     2641 gatgtggtac ggcttcttct cgttgatg
//
LOCUS       DASTEY010000291         8090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_582927, whole genome shotgun sequence.
ACCESSION   DASTEY010000291 DASTEY010000000
VERSION     DASTEY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582927"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFO72_05060"
     CDS             complement(<1..714)
                     /locus_tag="VFO72_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478688.1"
                     /translation="MSATDAHELESELAAANKAIENLVKEAAANENRANALSENAWRW
                     VFRYRRIQREYLAPVSRALRRLTGNAQAVSPVSTSHTNGTSAAARSEETLAKLLALVK
                     NEIRHGRNNAAASVAQLIADRFPQDPQAQRLFCGVETLERNDRTQLQQANHHLALAQA
                     YYLLGENEAAESNYWAALSFNPDLPEAHSGLAAIRMPGISYLEWLEKLYGYLKPENVI
                     EIGVSQSASLARIPPPAIAI"
     gene            773..1537
                     /locus_tag="VFO72_05065"
     CDS             773..1537
                     /locus_tag="VFO72_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HET9478689.1"
                     /translation="MIIIDRNKVVVSHGDDERREEYDIGSPEAFRVLSRLWLRSGWDN
                     KYVYSFTWLGRPIIQLPEDMIRIQEVIFAVRPDVIVETGVAHGGSLVFYAGLCKAMDR
                     GRVIGVDVEIRPHNRQAIESHALFSFITLIEGSSIEPAIVAAVNARVRPDEKVIVLLD
                     SCHQKQHVLEELRAYSTLVAPGSYIVAMDGIMEELVGAPRSSPDWSWNNPRQAALEFV
                     AENPNFVIEEPPFAFNEGTVTERVTYWPSAFIKRLN"
     gene            1555..4083
                     /locus_tag="VFO72_05070"
     CDS             1555..4083
                     /locus_tag="VFO72_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478690.1"
                     /translation="MVVIPTRNRSAIATNAINSVLDQPVEHMQVMVSDNSTSESEREA
                     LAAFCSTRDDNRLRYVRPPEPLSMTAHWQWAIEQALEFYQASHFTYLTDRMMFRTGAL
                     KEALAAVSLYPEKIVSYNMDRIVDHVRPIRVEQYPGTEKLLEIQTVDLSRLVSQSVFH
                     AALPRMLNCIVPREVFVQMQARFGNVFSSIAPDFHFCCRCMEMEETVLFYDRSFLFHY
                     ALDRSHGATVTRGEINPDAADFTANLPVDNSIRNFATPIPQLITAVNAIFQEYCLFKQ
                     ETGSPRFFEIDIGKYLQANADEIGEFTNPDLRAKTLALLVEKGYQAGAVKDDSKRVEI
                     TFQQRLRGKLKRMATSSGTTETWLFAARHFGIEPPGENSFEFATLDEAVDYAKNVSRG
                     NVSRNESQPRLIPTRELPTTVNGAHGSETQFANLRQALVSVVPLSFRRVLHERRNQVR
                     LATLLRTKSHFRASRKAAGSSHVPEGINLIGYIRADMGLGVAARGMASAFEAAGIPFN
                     VINLEHGNYSSHTDCSWSHKEVSTSRYDVTVVCVNPDNSFYLRTQVSSEILGNRYVIA
                     NWYWELPEVPDHWLKEFEYVDEVWAPSNFIREAIAKKAAVPIVRVPTVVNVKQRQRFS
                     RTQLGLPEHRFLFLAMFDTRSILERKNPLGVLRAFKSAFRAVDTSVGLVMKFNNANYD
                     QPLMRALQAELAGWENIFVVDRLLTRDELTSLIDACDCYVSLHRSEGFGLGPAEAMRL
                     GKPAILTNWSGSTDYVTHDNCVPIDYKLVELDRDYGPYKAGQQWADPDLEQAAHWMKR
                     IAAEPELAREIGLRGQQTINAEFSPKAVGRIIQARLDEIRSGEY"
     gene            4207..5022
                     /locus_tag="VFO72_05075"
     CDS             4207..5022
                     /locus_tag="VFO72_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9478691.1"
                     /translation="MTFFVRRIVNSINYRVLAPLTNRDARNWASKTDFRKTHRRLLQQ
                     LRNSHSHDEAVRLAVGGEFDAFGLLLREALVFHGLKPTDYLIDVGCGSGRLAKPLAEY
                     LKGKYLGIDVVPGLVQYARRLVPRPDWRFEVAEGLTIPEADQQAGLVCFFSVLTHLLH
                     EESFVYLREAKRVLKPGGKIVLSFLDFRVASHWGSFESNLRDIGLGSQPMNVFVCPDM
                     LREWAKRLEIEIETIHDGDESYLPLSKPITFEDGTVLKDRAAFGQSVCVMVRR"
     gene            5467..5604
                     /locus_tag="VFO72_05080"
     CDS             5467..5604
                     /locus_tag="VFO72_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478692.1"
                     /translation="MTPIVKVENLGKVYQIGTKPRPERRELEKFLDTPVKGYSSGMYM
                     A"
     gene            5961..6791
                     /locus_tag="VFO72_05085"
     CDS             5961..6791
                     /locus_tag="VFO72_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478693.1"
                     /translation="MNISPEPLVVIQPTRSWRLVSFKDLWSYRELLFFLTWRDVKVRY
                     KQTALGAAWAILQPLFMMVIFTIFFGRLAGVDSAGVPYPLFALAGLVPWTFFSNAITQ
                     SGNSLVGSSNLITKVYFPRLIVPTAAMLAGLVDFVLAFAMLCLMLIYYRVSVTSQVLF
                     LPVLVFLTALFALGVGMWMAALNVKYRDVRFALPFVIQIWLFVSSVILPSSSVPEKWR
                     WVLLLNPMSAIIEGYRAALLGLPFDWPAIGIAAVLTILVLLYAIYSFGRVERSFADII
                     "
     gene            6811..8061
                     /locus_tag="VFO72_05090"
     CDS             6811..8061
                     /locus_tag="VFO72_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478694.1"
                     /translation="MRPIIKVQNVSKRYYLGGPSAAYSTLREAVMDVARKPLRFLGRG
                     EEPQILWALKDVSFEVTPGDVVGIIGRNGAGKSTLLKILSRITEPTSGRVELYGRVAS
                     LLEVGTGFHPELTGRENIFLNGSILGMTRKEIESKFDEIVSFAEIEKFLDTPVKRYSS
                     GMYVRLAFAVAAHLEPEILIVDEVLAVGDAAFQKKCLGRMRDVASTGRTVLFVSHNMA
                     AVSSLCSKAMLLWEGKVQVPLGSVDDAVQHYLKQVHRITKTKLAERTDRSGTGKVRIN
                     DFGSFDREGNELEFVATGQEIDFRIYYSTNGARAENIVVGIGISAHSGTFVSLLSNEV
                     AGNAFDSIGKEGYFACKIKKFPLSPGNYTVSLIIRSNDVIADWIQHAATIVVEPGDFY
                     GSGRLPPQSHGGVLIEQDWDTISK"
BASE COUNT         1739 a         2173 c         2291 g         1887 t
ORIGIN      
        1 gatcgcgatc gcgggcggag gaatgcgtgc gagcgaagcg ctttgcgaga cgccgatctc
       61 gatgacgttt tccggtttga gataaccgta gagtttttcg agccactcca gataagagat
      121 gcccggcatt ctgatggcgg ccaggccgct gtgtgcttcg ggcaagtcag gattgaaaga
      181 aagcgcggcc caatagtttg actctgctgc ttcgttctcg ccgaggaggt aataggcctg
      241 cgccaaagcg agatgatggt ttgcttgttg gagctgcgtg cggtcgttgc gctctaaagt
      301 ttcaacgccg cagaaaaggc gctgcgcttg cggatcttgc ggaaaacgat ctgcaataag
      361 ttgcgctacc gaagccgcag cgttattgcg cccgtgccgg atctcgttct ttaccagtgc
      421 gagaagtttg gctagtgttt cctcagatcg agcggcagcg gaagtgccgt tggtgtgaga
      481 ggtcgaaacc ggtgaaacag cttgcgcatt cccagttaag cggcgcaatg ccctggaaac
      541 tggcgcgaga tactcccgct gaatgcggcg atagcgaaac acccagcgcc acgcattttc
      601 acttagcgcg ttggcgcggt tttcgttggc ggctgcttct ttgactagat tctcgatagc
      661 cttattggct gccgccaact cgctttcgag ttcgtgcgcg tctgttgcgg acactgccaa
      721 agaataccac agagacacac cgcatactgg ggtacaatcc catcacgaac caatgatcat
      781 catcgaccga aacaaggttg tcgtttcgca cggcgacgac gaacgccgcg aggaatacga
      841 catcggctca cccgaagcgt ttcgtgttct cagccggctg tggctgcgct cggggtggga
      901 caataagtat gtttatagct tcacgtggct ggggcggccg atcattcaac tgccggaaga
      961 catgatccgg atccaggaag taattttcgc tgtgcgtccc gacgtgattg tggaaacagg
     1021 cgtggcacac ggcggatcgc tggtgttcta cgccggcttg tgtaaggcaa tggatcgggg
     1081 ccgcgtgatt ggggtggacg tcgagattcg ccctcacaat cgccaagcga tcgaatcgca
     1141 cgcgctgttt tcgttcatca cgttgattga gggcagttcg atcgagccag cgattgtggc
     1201 tgccgtgaac gcgcgggttc gtccagacga gaaggtcatc gttttacttg attcatgtca
     1261 tcagaagcag cacgtactcg aggaactgcg agcttattcg acactagttg cgccaggctc
     1321 gtacattgtc gcgatggatg gcatcatgga agaactggtc ggcgcgccgc gcagttcgcc
     1381 tgactggtcg tggaacaacc cgcggcaagc ggcgctggag ttcgtggcgg aaaaccctaa
     1441 cttcgtcatc gaagaacctc cgttcgcttt caatgagggt actgtcaccg aacgcgttac
     1501 ttactggcct tctgccttta tcaaacgcct gaactgatgg tttacacggg gacagtggtg
     1561 gtgattccga cgcgcaaccg cagcgcgatc gctacgaatg ccatcaattc cgttctcgat
     1621 cagccggtgg agcatatgca ggtgatggtc tccgacaact ccacgtcgga gagtgagcgg
     1681 gaagctctcg ctgctttctg ttccacgcgt gacgacaatc ggctgcgtta cgtgcgaccg
     1741 ccggaaccgc tgtcgatgac cgcgcattgg cagtgggcaa tcgagcaggc gctcgagttt
     1801 tatcaagcga gtcactttac ctatctcacg gaccggatga tgttccgcac cggcgcgctg
     1861 aaagaagcct tggcggctgt atcgctctac cctgagaaga tcgtcagcta caacatggat
     1921 cgcatcgtcg atcacgtcag gcccattcgc gtggagcagt atcccggcac agagaagttg
     1981 ctcgaaattc aaacggttga tctgtcgaga ctcgtgtcgc aatcagtctt tcacgctgcg
     2041 ttgccgcgca tgctcaattg catcgtgccg cgagaagtgt ttgtccaaat gcaagcgcgg
     2101 tttggaaacg tcttctcgtc gatcgcgccc gatttccatt tctgctgccg ctgcatggag
     2161 atggaagaga cggttctttt ttacgatcgc tcgtttctgt ttcattacgc cctcgatcgc
     2221 agtcacggcg ccacggtcac tcgaggcgaa ataaatcctg acgccgccga cttcaccgcg
     2281 aaccttccgg ttgacaattc gatcagaaac tttgcgacgc cgatacctca actcattacc
     2341 gccgttaatg cgatcttcca ggagtattgt ctttttaaac aggagacagg cagcccgcgt
     2401 tttttcgaga tagacatcgg gaagtatctt caggccaatg ctgatgagat cggggagttc
     2461 acaaatcccg atttacgggc taagactctg gcgctgctgg tcgaaaaagg ctatcaggcg
     2521 ggggcagtaa aggacgattc gaaacgcgtc gagattacct tccaacagag actgcgcgga
     2581 aaattgaagc gcatggcgac ttcctctggt acgaccgaaa cgtggttatt cgctgcccgc
     2641 cattttggga ttgagccacc aggcgagaat tcgtttgagt ttgccacgct tgatgaggcg
     2701 gtcgattacg caaagaacgt atctcgcggc aacgtttccc gcaacgaatc tcagccccgt
     2761 cttattccga cgcgtgaatt accgacaacc gtcaacggag cgcacggatc cgaaactcaa
     2821 ttcgcgaacc ttcgccaggc gcttgtatcc gtggttccac tatccttcag aagggtgctg
     2881 cacgaacgaa gaaaccaggt acggctagcg acactgctgc ggaccaagag ccattttcgc
     2941 gcttcacgga aagccgcggg gtcttcgcac gttcccgaag gaatcaatct tatcggctac
     3001 attcgcgctg acatgggtct cggcgtcgcc gcacgtggca tggcctccgc attcgaagcc
     3061 gcgggtatcc ccttcaacgt gatcaacctg gagcatggca actactcgtc gcacaccgat
     3121 tgctcgtggt cgcacaagga ggtctcgacc tcgcgttacg acgtcactgt tgtttgtgtg
     3181 aatcccgata acagctttta tcttcggaca caggtctcat ctgagatact cggaaatcgt
     3241 tacgtgatcg ccaattggta ttgggaactg ccggaggtgc ccgatcactg gttgaaggag
     3301 ttcgagtatg tcgacgaagt gtgggcgccc tcaaacttta tccgtgaagc gattgcgaaa
     3361 aaggctgcgg tacctatcgt gcgcgttcct acggtagtga acgtgaagca gcgccaaagg
     3421 ttttcacgca ctcagctcgg tctacccgaa caccgatttc tgttcctggc catgtttgac
     3481 acgcggagca ttttggagcg aaagaacccg ttgggcgtgt tgcgtgcatt caagagcgca
     3541 tttcgcgctg tcgatacgag tgtcggactg gtgatgaagt ttaacaatgc caactacgat
     3601 caaccgctaa tgcgcgcgct acaagccgag ctcgcggggt gggaaaacat ctttgtagtc
     3661 gatcgtcttt tgacgcgtga cgaactgacg tcgcttattg atgcctgtga ttgctacgtt
     3721 tcgctgcatc gttcggaagg cttcggactc ggtccagcgg aagcgatgcg cctgggcaag
     3781 ccggcgatcc ttaccaactg gtcgggcagc accgattacg tgacgcacga caactgcgta
     3841 cctatcgact acaagctcgt tgagttggac cgcgattatg gtccatacaa agcaggccag
     3901 caatgggcgg atccggatct cgaacaagcc gctcactgga tgaaacgaat cgctgccgaa
     3961 ccagagctgg cgcgagaaat tggcttgcgc gggcagcaga cgatcaatgc ggagttttcg
     4021 cctaaggccg tgggccggat tattcaagct cgcctcgacg aaatccggag tggggagtac
     4081 taatgcggca taacccggga ccggaggcgt ctcgcccgca ctcgtcacac cgagctaccg
     4141 gcgaaacccg gcaggcgaga cgcctgcggt cccagggtat cggtacatga tgaccagagg
     4201 tatgaggtga ctttcttcgt tcgcagaatc gtcaacagca tcaactaccg cgtgcttgcg
     4261 ccgctgacga atcgcgacgc gcgcaactgg gcgtcgaaga ctgactttcg gaaaacgcac
     4321 cgccggctcc tgcaacaact ccggaattcc cacagtcacg acgaagccgt cagactcgcg
     4381 gttggcggcg agttcgatgc tttcggtctg ctcttgcgcg aggctctcgt tttccacgga
     4441 ttaaaaccca cggattattt gatcgatgtc gggtgtggtt cgggacggtt ggcaaaaccg
     4501 ctggcggagt atctcaaagg aaaatacctg ggcatcgacg tcgtgccagg tctggtccaa
     4561 tacgcgcgca ggctcgtgcc acggcccgac tggcgttttg aagtggcgga aggtttgacg
     4621 atacccgaag cggatcagca agccggcctg gtttgtttct tctcggtgct gacacacctg
     4681 ttgcacgagg aatcgtttgt ctacctgcgc gaggcgaagc gcgtgttaaa gccgggcggc
     4741 aagatcgtgc tgtcgtttct ggatttccgc gtggcgagtc actggggttc gtttgaaagc
     4801 aatctgcgcg acatcgggct gggctcgcaa ccgatgaacg ttttcgtctg tcctgacatg
     4861 ttgcgtgaat gggcgaagcg tctcgaaatc gagatcgaaa caatccatga tggcgacgag
     4921 tcttacctgc cgctctcaaa gcccatcact ttcgaagacg gcacggtgct aaaagatcgt
     4981 gccgcctttg gacaatctgt gtgcgtgatg gtacgaagat aatgatgaac cccgagccgc
     5041 ttgttgtcat tcaaccgaac caaaactgga gactggtcag cctaaaagac tcctactgac
     5101 atagcgcacc ttgatcctgg gaccgcaagc gtctcgcctg caatcgtcgc ctcgggctac
     5161 ctacgaaaca cacaggcgag acgcctacgg tcccggcttg ctggttctgt tcgtaccact
     5221 cgatcgtgac cggccgcgaa atcctgcgcc gcctgttacc acgattctct tgtatggaaa
     5281 ctccggttcg tgcccatcca tgcgccggag aataccaaat ttgccgagag acgacccata
     5341 agttaaggta taccctttgt taaaacgcca cggatctctg gctcaaattc caaggtaaaa
     5401 tgcgttacgg taaaagcaat ccacagatta cgctgaaagt aatctgtgaa atggtggatg
     5461 ggttgtgtga cacctatcgt taaagttgaa aacctcggta aggtttatca gatcggaacg
     5521 aagcctcggc cagaacggcg ggaactggaa aagtttctcg acacgcccgt caaaggttac
     5581 tcgagtggca tgtatatggc ataagtatta gctcgcctga caacgtcgtc atcgtggagt
     5641 gtcgcaccag ccattcctac ggtgcgattg accagttggc gcccgcgagt acttcatccg
     5701 atgtcgtatc gaacagaaca tcttgtgtcc cggcctttat ttcttgaacg tcggcgcgcg
     5761 gtctgcgaac aagtatctgg actttgtgcc ccaggcaatg actttcgaaa tctattccga
     5821 tgagttcgtc gcttctgttt ggttgagcga cgttggaggg tacgtgcgag ttccctccga
     5881 atggacggag ccgcaagctc ttatggaaca tgccggccga ttggacaatc tgtgtgtgtg
     5941 atgatacgta actagggccg atgaatatat cccctgagcc gctcgtcgtt attcaaccaa
     6001 ctcgaagttg gaggctcgtc agcttcaaag atctctggtc gtatcgcgag ctacttttct
     6061 ttctgacgtg gcgcgacgtc aaagtgcgct acaaacagac cgcgcttggt gccgcctggg
     6121 ccatcctgca accactgttt atgatggtca tattcacaat cttcttcggc cgactcgccg
     6181 gcgttgactc cgcgggcgtt ccgtacccac tgttcgcact cgccggcctc gttccgtgga
     6241 cgtttttctc caacgcgatt acccaaagcg gtaacagcct cgtcggaagc tcaaacctga
     6301 ttacgaaagt ttacttccca cgactcattg ttccgactgc ggcaatgctc gcgggcctgg
     6361 tcgacttcgt acttgccttt gcgatgcttt gcctgatgtt gatctactac cgcgtctccg
     6421 taacttcaca ggttctgttt ctccccgtgc ttgttttcct tactgcactt ttcgcactcg
     6481 gagtcggtat gtggatggct gcgttaaacg tgaagtatcg cgacgtgcga tttgcgttgc
     6541 cctttgtcat acagatctgg ctgtttgtgt cgtccgtcat tctcccgtcg tcttcagtcc
     6601 cggaaaagtg gcgttgggta ctgctgttga atccaatgtc ggccatcatc gaaggctatc
     6661 gagccgccct ccttggactt cctttcgact ggccggccat cggcattgct gccgttctaa
     6721 ccatcctggt actgctctat gccatctatt cgtttggacg cgtggaacgc agcttcgccg
     6781 acattatcta gcgacgttag cgcgcgcagc atgcgaccaa tcattaaagt tcagaatgtg
     6841 agtaaacgtt attaccttgg ggggccatcc gcggcctact cgacgctgcg cgaagccgtg
     6901 atggacgtgg cgcgtaagcc gttgcgcttt cttggtcgcg gagaagaacc gcaaatactc
     6961 tgggcgctca aggacgtgag ttttgaagtc acgcccggcg acgttgtcgg catcatcggt
     7021 cgtaacggcg cgggcaagtc gacactgctc aaaatacttt cgcgcatcac ggaaccgact
     7081 tcaggccgcg ttgaactcta cggccgcgtg gcgagcctgc tcgaagtggg cactggtttt
     7141 catccggagc tcactggccg cgagaacatc tttctcaacg gttcgattct gggtatgacg
     7201 cggaaagaga tcgaatcgaa gtttgacgag attgtctcgt tcgccgagat cgaaaagttt
     7261 ctcgacacgc cggtcaaacg ttacagcagc ggcatgtacg tacgtctggc gtttgcggtg
     7321 gccgcgcacc tcgaaccgga gatactgatc gtcgatgaag tgctggccgt gggcgacgca
     7381 gcgtttcaaa agaaatgcct gggccgcatg cgtgacgttg caagcacggg tcgcacagtg
     7441 ctcttcgtga gccacaacat ggctgccgtt tcctcactct gttcgaaggc aatgttgctg
     7501 tgggaaggaa aggtccaagt accgctgggc agcgtggacg acgcggtgca gcactacctc
     7561 aagcaggttc acaggatcac gaagaccaaa ctcgccgagc gcacagatcg atccggtacg
     7621 ggaaaggttc gcatcaacga ctttggcagc ttcgatcgtg aggggaacga gctcgaattt
     7681 gtggccacgg gacaggagat cgactttcga atctactact cgacgaacgg agccagggcg
     7741 gagaatattg tggtgggaat tggcattagt gctcattcgg gtacctttgt tagcttgctt
     7801 agcaacgaag ttgctggtaa tgctttcgac tcgatcggga aggaaggtta cttcgcctgc
     7861 aagatcaaaa aattcccgct ttcgcctggc aattacacgg tgagtttgat aatccgttca
     7921 aatgacgtga tcgccgattg gattcaacac gccgccacga tcgtcgtcga acccggagat
     7981 ttttatggca gcgggcgctt gccgccgcag tcgcatggtg gagttttgat cgaacaggac
     8041 tgggacacga tttcaaaatg aaagctttga tcaaaagggt gttttcgata
//
LOCUS       DASTEY010000292         4823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_846740, whole genome shotgun sequence.
ACCESSION   DASTEY010000292 DASTEY010000000
VERSION     DASTEY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846740"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFO72_05095"
     CDS             <1..756
                     /locus_tag="VFO72_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9478695.1"
                     /translation="LEMGGKNAIIVMDDGDIDLAVEGAIWGAFGTSGQRCTASSRLVV
                     HKKVYKQFVTKLVEEAKALRVGNGADPKTDVGPLINNDAVEKVMSYIRIGRNDDGAKL
                     ACGGKRLSRGDYANGYFIEPTVFTDATPDMRISQEEIFGPVTTVIPANSLEDAIQIAN
                     GVRYGLSAAIYTQNVNRAFKAMNEMYTGIFYVNASTIGAEVHLPFGGTKATGNGHREA
                     GTQVLDIFSEWKSIYVDFSGKLQRAQIDVESPQ"
     gene            808..1953
                     /gene="ald"
                     /locus_tag="VFO72_05100"
     CDS             808..1953
                     /gene="ald"
                     /locus_tag="VFO72_05100"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9478696.1"
                     /translation="MIVGLPKEIKDNEYRVGLTPAGVRALKDAGHQIMVERDAGAGSG
                     FENALYERAGATIIDSADEVWGQAEMIVKVKEPIAQEYPRMREGQLLFTYLHLAPDRE
                     LTRQLLDRKVTGIAYETITDRRGQLPLLTPMSEVAGRMAIQVGAHYLEKMSGGRGILL
                     GGVPGVPAARVVIIGGGVVGTNAAKIAVGMGAHVTIIDNNLDRLRELDDIFLSKISTL
                     ASSAYMIQDAISQADLIVGAVLVKGAAAPKLVTRSMLKEVPNGALIVDVAVDQGGCIE
                     TTHPTTHSNPTYYVEGVLHYCVANMPGAVPRTSTFALTNATLPYALKLANKGFLHAIA
                     SDPGLKEGVNTFAGECTYEAVATDQGIPYTPLDQLIGALNADLANAR"
     gene            complement(2076..2495)
                     /locus_tag="VFO72_05105"
     CDS             complement(2076..2495)
                     /locus_tag="VFO72_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478697.1"
                     /translation="MITQEKVSRARRQAVHATKTPRRAPCVLLAEDDAALRRYIEVLL
                     QRAGYEVVSAADGLEAMKSLLTVTVDVVLTDAVMPNVSGYELCRFVRSSERLSHLPII
                     LLSGLDPKNAETEQVDAFLAKPITPESLLACIRKVCV"
     gene            complement(2643..4580)
                     /gene="asnB"
                     /locus_tag="VFO72_05110"
     CDS             complement(2643..4580)
                     /gene="asnB"
                     /locus_tag="VFO72_05110"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9478698.1"
                     /translation="MCGIAGIISLDPGPKIERMLEALEHRGRDDEGVWISESIDQHNR
                     RACLGHRRLAIIDPSPAGHEPMLSHNGRYVLTFNGEIYNYRELREELRAAGHQFRTGT
                     DAEVLLASIVEWGWNAIDRLNGMFAFAVWDNQERTLTLARDHVGIKPLYYALIPCNDG
                     APPAFVFASEIKAILATRLIKPELEPEALHQFLTFLWAPDPNTLFAGIKTVPPAHLLH
                     FRDDEIKLHQWWDVSFTHIEEGRNEAWWRERTLETLDRVVKMEMVADVPLGSFLSGGI
                     DSSGITALMQRHVDGQRISTYTVGIENKDLRYDIIPDDVRWARRVNEQLQTDYHEIVL
                     QPSVAELLPKLVYHMEEPPIDMAIPTYLISRAARETLTVMLSGMGGDEVFAGYPRQMA
                     MQIASAFDPVPQLLRRPLMRTVAGALPGGLPGKLTAPLRNAKKFARSAALDFEDRYLG
                     YGTYFTDEMKHRLYSDEWRAITRDHDAYATHRSYFAAAANAAPLNRLLYVDLKTFLPC
                     LNLMTTDKMSMASNLEVRVPFLNREMIEMAARMPPRLKLHGLKRKYILKRAFEGVLPK
                     DVIWRRKAGFGAPIRSWLRGPLRPLVADLLSEDVVKRRGLFRPREVKRVIDANFSGRE
                     DYNLHVFQLLGLELWHRTFLD"
     gene            4579..>4823
                     /locus_tag="VFO72_05115"
     CDS             4579..>4823
                     /locus_tag="VFO72_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478699.1"
                     /translation="MSDFRSQITDLKGPVLAERIEDPESGTSCSDQFIYGDWRLCTLH
                     HARADLPDLFLLTLVLRFQSDLLFTPFFVKRCAAEIV"
BASE COUNT         1050 a         1359 c         1399 g         1015 t
ORIGIN      
        1 cttgagatgg gcggcaagaa cgcgatcatc gtgatggacg atggcgatat cgatctcgcg
       61 gttgaaggcg ccatttgggg cgccttcggt acgtcagggc aacgttgcac tgcttcgtcg
      121 cgcctggttg ttcacaagaa agtttacaaa cagttcgtca ccaagcttgt cgaagaggcc
      181 aaggcgttgc gcgtcggcaa cggcgccgat ccgaaaacgg atgttggacc attaatcaac
      241 aacgacgcgg tcgagaaagt gatgagctac attcgcatcg gacgcaacga cgacggcgcg
      301 aagctcgctt gcggcggcaa acgcctcagc cgcggtgatt acgcaaacgg ttattttatt
      361 gagccaaccg ttttcactga cgccacgccc gacatgcgca tctcgcagga agagatcttc
      421 ggtccggtaa cgaccgttat tcccgcaaac tcgcttgaag acgcgattca aatcgcgaac
      481 ggagtccgct acggactttc cgccgcgatc tacacgcaaa acgtcaatcg cgctttcaag
      541 gctatgaacg aaatgtatac cggcatcttc tatgtgaacg cttcgacgat cggcgccgag
      601 gtgcatctcc cgtttggcgg cactaaagcc accggtaacg gccatcgaga agccggcact
      661 caggtgttgg atattttctc ggaatggaag tccatctacg ttgacttcag cggcaagctg
      721 caacgcgcgc agattgacgt cgagtcgccc cagtaatcgc gcctttatct gtgttgctgc
      781 tattgagact atattggaga acaaaccttg atcgtcggtc tgcccaaaga aatcaaagac
      841 aatgaatata gagtcggatt aactcccgcc ggtgtgcgcg cgctcaaaga cgcgggccac
      901 cagattatgg tcgaacgaga cgcgggcgca ggctcaggct tcgaaaacgc gctttacgaa
      961 cgcgccggcg caacaattat cgattcggct gacgaagtct ggggccaggc cgagatgatc
     1021 gtgaaggtga aagaaccgat cgcgcaggag tatccgcgaa tgcgcgaagg acagttgttg
     1081 ttcacctatc tccacctcgc gcccgatcgc gaactgacgc gacaattact cgaccgcaaa
     1141 gttaccggta tcgcttacga gacgatcacc gatcgacgtg gacagttgcc gttgttgacg
     1201 ccgatgtctg aagtggccgg ccgcatggcg atccaggtcg gcgcacacta tctcgagaag
     1261 atgagcggtg gacgcggaat tctgctgggt ggtgttcccg gtgttcccgc cgcgcgcgtg
     1321 gtgattatcg gtggcggtgt ggttggtaca aacgcggcaa agatcgcggt cggcatgggc
     1381 gcgcacgtca cgatcatcga caacaatctc gatcgattgc gcgagcttga cgacattttc
     1441 ctttccaaga tctccacgct cgcgtcgagc gcctacatga tccaggacgc gatctcgcag
     1501 gccgatctga tcgtcggcgc tgtgctcgtg aaaggtgcgg cggcgcccaa actggtcacg
     1561 cgaagcatgc tcaaggaagt tccgaacggc gcgttgatcg ttgatgtcgc ggtcgatcag
     1621 ggtggctgca tcgagaccac gcatccgacc acgcactcaa atccgactta ctacgtcgaa
     1681 ggagtgttgc actactgcgt cgccaacatg ccgggcgccg tgccgcgcac gtcgaccttc
     1741 gcgttaacaa acgcgacgct gccgtatgcg ctgaaactgg cgaataaagg gttccttcac
     1801 gctatcgcga gcgatccggg tctgaaggaa ggcgtgaata cttttgccgg tgagtgcacg
     1861 tatgaggcgg tcgctacgga ccagggaatt ccctataccc cgctcgacca gctaatcggc
     1921 gccctgaacg ccgaccttgc aaatgcgcgc taagctgctt tcgagtttct tctacatgag
     1981 tttcccgcca gtgggagacc agcaaaagaa aaattatttt aatttccttt tccatgagtc
     2041 tcacgcttac gggtctgcag tgaaaaagaa acttcctaaa cgcagacctt gcgaatgcac
     2101 gcaagtagac tctctggcgt gatgggtttg gcgagaaacg catcgacctg ctcggtttcc
     2161 gcgttcttcg gatcgagtcc acttaacagg attatcggca gatgactgag acgctcactg
     2221 ctcctcacaa agcggcataa ttcataacca ctgacattcg gcatcaccgc atccgtcaac
     2281 accacatcaa cagtcacggt caacaacgac ttcatcgcct ccaaaccatc cgccgccgaa
     2341 acgacctcgt aaccggcacg ttgcagcaag acctcgatgt agcgccggag agcggcgtcg
     2401 tcttcagcga gaagcacaca gggagcgcgc cgcggcgttt tcgtcgcatg cactgcctgg
     2461 cgtctggccc tggaaacttt ttcctgtgtg atcattggag cttgtagaag cgagactttc
     2521 gaggccgtga gggctctgaa ggtcgggcgc gactaaggga gagactcgcc agaagcagat
     2581 tagactgccg ccgctgcgat tgtcaatgtt ttctcttgtt aatcccccgg caaaaccgcg
     2641 gatcaatcaa ggaaggtgcg gtgccagagt tccagaccta aaagctggaa aacatggagg
     2701 ttatagtcct cacgtccgga aaagttagcg tcgatgaccc gttttacctc cctcgggcgg
     2761 aacaggccgc ggcgtttcac cacatcctcc gaaagcaggt cggcaaccag cggccgtaga
     2821 ggacctcgta accacgaccg gatcggcgct ccaaagcccg cctttcgccg ccagatcacg
     2881 tccttcggta aaactccttc gaacgcgcgt ttcaggatgt acttccgctt cagtccatga
     2941 agtttcaacc gtggcggcat gcgtgcggcc atttcaatca tttcgcgatt cagaaatggc
     3001 acccgcacct ccaggtttga cgccatcgac atcttgtcgg tcgtcatgag gttcaggcac
     3061 gggaggaacg tcttcaggtc gacgtagagg agacgattga gcggtgcggc attcgcagcc
     3121 gctgcaaaat aagagcgatg cgttgcgtag gcgtcgtgat cgcgcgtgat cgcgcgccac
     3181 tcgtccgaat acagccggtg tttcatttcg tcggtgaagt aagtgccgta gccgaggtag
     3241 cggtcttcga aatcgagcgc cgcgctgcgc gcaaactttt tcgcattgcg taagggcgcg
     3301 gtgagtttgc cgggcaagcc gccgggcaag gcgcccgcaa cggttcgcat caacgggcga
     3361 cgcagtagtt gcggcaccgg atcgaatgcg gaggcgatct gcatcgccat ctgccgcggg
     3421 taaccagcga agacttcgtc gccgcccata cctgacaaca tcaccgtcaa tgtttcgcgc
     3481 gcggcgcgtg agatcaggta agtcggaatc gccatgtcga tcggcggctc ttccatgtgg
     3541 tagacgagct tcggcaggag ttccgcgact gacggttgca acacgatctc gtgatagtca
     3601 gtctgcaact gttcgttgac gcgccgggcc cagcgcacgt cgtcgggaat gatgtcgtag
     3661 cgcaggtcct tgttctcgat gccgacggtg taggtgctga tgcgctggcc gtcgacgtgc
     3721 cgctgcatca gcgcggtgat gcccgaagaa tcgatgccgc cggagagaaa ggatcccagt
     3781 gggacgtcgg ccaccatctc catcttgacg actcgatcga gtgtttcgag tgtacgttca
     3841 cgccaccacg cttcgtttcg accctcttcg atatgtgtga acgagacgtc ccaccactga
     3901 tgaagtttga tctcgtcgtc acggaagtga aggaggtgtg ccggtggaac tgtcttgata
     3961 ccggcgaaaa gtgtgtttgg atcgggcgcc cacaggaatg tcaggaactg atgtaacgct
     4021 tccggctcga gctccggctt tattaatcgc gtcgcgagaa tggctttgat ttccgacgcg
     4081 aatacaaacg ccggcggcgc gccgtcgttg cagggaatca aggcgtaata gagtggcttg
     4141 attccgacgt gatcgcgcgc gagcgtcagc gtgcgttcct ggttgtccca aacggcaaaa
     4201 gcaaacatac cgttgagacg gtcgatcgcg ttccaacccc attcgacaat cgaggcaagc
     4261 aggacttcgg cgtcagttcc ggtgcgaaac tggtggccgg ccgcgcgcag ttcttcacgc
     4321 aactcgcgat agttgtaaat ctcgccgtta aacgtgagaa cgtagcggcc gttgtgcgaa
     4381 agcatcggct cgtgaccggc agggctggga tcgattatgg caaggcggcg atggcccagg
     4441 caggcgcgcc ggttgtgttg gtcgatcgac tctgaaatcc aaacaccttc atcatcgcga
     4501 ccgcggtgct cgagcgcttc gagcatccgt tcaatctttg gcccaggatc caggctgatg
     4561 attcccgcga tgccacacat gtcagatttc agatctcaga tcacagatct caaagggccg
     4621 gtgctcgcgg agagaatcga ggatccggaa agtggcacga gttgtagcga tcaattcatt
     4681 tatggcgatt ggcgcctctg taccctgcat cacgcacgcg cagacttgcc ggatctgttc
     4741 ctgctgaccc ttgtcctgcg ctttcaaagt gatctgctct tcacgccctt ctttgtgaag
     4801 cgttgcgcgg cggaaatcgt cga
//
LOCUS       DASTEY010000293         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_39347, whole genome shotgun sequence.
ACCESSION   DASTEY010000293 DASTEY010000000
VERSION     DASTEY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39347"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2013
                     /gene="uvrB"
                     /locus_tag="VFO72_05120"
     CDS             <1..2013
                     /gene="uvrB"
                     /locus_tag="VFO72_05120"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET9478700.1"
                     /translation="YPFYYFSHMRFKLKSEYRPKGDQPAAIDALVHGLNDGAKDQVLL
                     GITGSGKTFTIASVIERVQRSTLVLAHNKTLAAQLFQEFRTLFPENAVEYFVSYYDYY
                     QPEAYVPAADVYIEKEAIINEEIDRLRMSATRALFERRDVIVVASVSCIYGLGDPDAY
                     YGLLFFVEPGGRMSRDVLLQRLVELQYERSDVNFQRGTFRVRGDVIEIYPSYQDLAYR
                     IELWGDEIDSISTIDPLLGEVVEKHTSRVPIYPKTHYVMSRPTIKRAIKTIREELEWW
                     EPKLIAEGKLVEAQRLHQRTMFDLEMIKEMGFCRGIENYSRHLTGKKPGEPPPTLLDY
                     LPRDTLIVIDESHQSIPQVRGMFEGDQSRKRTLVEYGFRLPSALDNRPLNFKEFEARI
                     GQTIYVSATPGPYELTKSSGEVVEQIIRPTGLMDPEVEVHPVKGQIDHLLEECRQRAE
                     RRERVLVTTLTKRMAEDLTEYFTEVGVRVRYLHSDIETLERIKILRDLRRGEFDVLVG
                     INLLREGLDLPEVSLVAILDADKEGFLRSEQSLIQTIGRAARNARGKAILYADRITDS
                     MKRAIEETQRRRAIQEVFNRENGITPQTIVKPIEATLITASEADYFKLPTEVDQIEDY
                     SPEHLEATISRLEVEMRAAAKRFEFEKAAELRDRIKVLRERELQLT"
     gene            2049..>2362
                     /locus_tag="VFO72_05125"
     CDS             2049..>2362
                     /locus_tag="VFO72_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9478701.1"
                     /translation="MQTKSNADEIQSFLSDASFIRGGYADRVVLPESIEEVAEVLAAA
                     DRDRVPVTVSGAGTGTVGGRVPFGGIVLATDRLNRIKRVSPNEHGFGRAVAEAGVILS
                     DF"
BASE COUNT          520 a          620 c          711 g          511 t
ORIGIN      
        1 tatccgttct actatttctc tcacatgcgt ttcaaactta agtctgaata tcgacccaag
       61 ggtgaccagc cggctgcgat cgacgcactc gtgcacggcc tcaacgacgg cgcgaaggat
      121 caagtactgc tgggcattac cggcagcggc aagacgttca cgatcgcgag cgtgatcgaa
      181 cgcgtgcaac gttccacgct cgtgttggcc cataacaaga cgctcgccgc gcagttgttc
      241 caggagtttc gcacactctt tccggagaac gcggtcgagt acttcgtttc gtactacgat
      301 tactaccaac cggaagcgta cgttcccgcc gccgacgtct acatcgaaaa agaagccatc
      361 atcaacgagg agatcgatcg cttgcgcatg tcggccacgc gagcgttgtt tgaacgtcgc
      421 gacgtgatag tggtggcgag cgtctcgtgc atctacggtc tcggcgatcc ggacgcgtat
      481 tacggtctgt tgttctttgt cgaaccaggc gggcgcatgt ctcgcgacgt cttgcttcag
      541 cgcctcgtcg agttgcagta cgaacgttcg gacgtcaact ttcagcgcgg cacttttcgc
      601 gtgcgtggcg atgtcatcga gatctatccc agttatcagg atctcgctta tcgcatcgag
      661 ttgtggggcg acgagatcga ttcaatatca acgatcgatc cgttgctcgg cgaagtggtc
      721 gagaaacaca cgtcgcgcgt gccgatctat ccgaagacgc actacgtgat gtcgagaccg
      781 acgatcaaac gcgccattaa gacgattcgt gaagagctcg aatggtggga gcctaagctg
      841 atcgcggagg gcaagctggt ggaagcgcaa cggctgcacc agcgcacgat gtttgatctc
      901 gaaatgatca aagagatggg cttttgccgc ggcattgaaa actattcgcg gcacctcacc
      961 ggtaaaaaac cgggcgaacc accgccgacg ttactcgatt acctgccgcg cgacacgttg
     1021 atcgtaatcg acgaatctca tcagagcatt ccacaggtgc gcggcatgtt tgaaggcgat
     1081 cagtcgcgca aacgcacgct ggtcgaatac ggatttcgat taccgtccgc gcttgataat
     1141 cggccgttga acttcaagga gtttgaagca cggattgggc agacgatcta tgtgtcggcg
     1201 actcccggtc catatgagct gacgaagtcg agcggtgaag ttgtcgagca gatcattcgt
     1261 ccgactggat tgatggatcc ggaagttgaa gtacatccgg tcaaaggaca gatcgatcat
     1321 ctgctggagg aatgccgcca gcgcgccgag cgacgcgagc gcgtgctggt cacgacgctc
     1381 acgaagcgga tggctgaaga cctgacggaa tatttcacgg aagtgggcgt gcgggttcgt
     1441 tatttgcact cggacattga aactctcgag cgcatcaaga tcctgcgcga cttgcgccgc
     1501 ggcgagtttg acgtgctcgt gggtatcaat cttctgcgtg agggattgga tttgccggaa
     1561 gtctcgctgg tcgcgattct tgatgcggac aaagaaggat ttctgcgttc ggagcagtcg
     1621 ttgattcaga cgatcgggcg tgcggcgcga aacgcgcgcg gtaaagcgat tctctacgcc
     1681 gaccgcatca cggattcgat gaagcgggca attgaggaaa cgcaacggcg ccgggcgatt
     1741 caggaagtct tcaatcgcga gaacgggatc acgccgcaga cgatcgtcaa gccgatcgaa
     1801 gccactttga tcacggcgtc tgaagccgac tatttcaagc tgccgaccga agtcgaccag
     1861 atcgaggact attcgccgga acatttggaa gcgacgattt cgcggttgga agtggagatg
     1921 cgtgcggcag cgaagcgatt cgagttcgag aaagcggcgg agctgcgcga ccggatcaag
     1981 gtgctgcgcg aacgcgagct acaacttact taaaatccgt gacggtccgt gttaatcggt
     2041 ggccctctat gcaaacgaag tcgaatgccg atgagattca gtcctttctg tctgacgcca
     2101 gttttatacg cggcggttac gccgatcgcg ttgtcttgcc agagagtatt gaagaggttg
     2161 ccgaagttct tgcggctgcg gatcgcgatc gcgtgccggt gacagtttca ggcgccggta
     2221 caggcacagt cggcggacgt gttccttttg gcgggatcgt gttggccacg gatcgactga
     2281 accgcataaa gcgcgtttcg ccgaacgaac acggatttgg acgcgctgtc gccgaggcag
     2341 gtgtaatctt gtctgacttc ca
//
LOCUS       DASTEY010000294         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1555368, whole genome shotgun sequence.
ACCESSION   DASTEY010000294 DASTEY010000000
VERSION     DASTEY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555368"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFO72_05130"
     CDS             <1..508
                     /locus_tag="VFO72_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478702.1"
                     /translation="VAREGKVVDIPGANIQWNYRHTSFQEGELLLGATLRLKHADPEQ
                     IKARMDDAKSRRLATQPHGSRSAGCFFKNPPASTTGTGKIIDEMGMKGQRRGSAIVSP
                     VHANFIVTEGENARAEDALALAEEIRERVKREQGIELEYEVELWRANPEPPTATENTD
                     QTRKEEER"
     gene            505..1416
                     /locus_tag="VFO72_05135"
     CDS             505..1416
                     /locus_tag="VFO72_05135"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9478703.1"
                     /translation="MRETVVPQKVGNRTGIGKARSGMGQRPARRAPAESIGARFRSLL
                     GYVPTALRIAAIAIACLLVVVVYRAAASASFFQIKKVETKGAARASVEDIQAVVRRDV
                     AATGVWRANLDEISQHLERLPWVRSAIVTRVLPDGIRVRITERQPRAVVRNSSGRFVW
                     VDDDAVFLGEMASTDQMPPFFLRGWSEEAGAQAENRQRVAKFLALQKDWEEQGIAERV
                     SEVNVMDLRDLRVQLAGDDSQIEVRLGGQDQGARLKKALTVLDAQRQTARGPLISYID
                     LSQGKRAIVGLTTGNQTVAEGTPPAQR"
     gene            1431..2693
                     /gene="ftsA"
                     /locus_tag="VFO72_05140"
     CDS             1431..2693
                     /gene="ftsA"
                     /locus_tag="VFO72_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET9478704.1"
                     /translation="MAKRTQHTIGLDIGTSKVTCIAGEPGEGGLLEIVGIGEADARGL
                     RKGVIVNPDAAVDAIKKAVEEVERMSGLEAEEVTINLAGSHILGFNGQAIVAVSGRDR
                     EIVAEDVRRAIDSACAIQLPAGREIVDRLPQEFIVDDQDGINDPVGMIGARLAVKVHI
                     VTSPVTARQNAINAVNRAGLVVADMVLEQLAAAEASLTEDDKEFGAALVDVGAETTGL
                     IIYQRGAVQHTAVFPLGGSHFTNDIAFGLRTPIPEAEKIKRTVGCACSSSLSEMERGE
                     LVDVPSVGGRPPRQLSRQILCDILQPRAEEVLMHVADEIKDAGWERQLSSGVVLTGGG
                     ALLSGMTEIAEQVFDAPVRLGYPERDRFGGLIEDIQSPAWAAAAGLALLAYRTQAAEI
                     RTGVSRRGSTGKLTHFVSRFKNRFGSIF"
     gene            2827..>3876
                     /gene="ftsZ"
                     /locus_tag="VFO72_05145"
     CDS             2827..>3876
                     /gene="ftsZ"
                     /locus_tag="VFO72_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET9478705.1"
                     /translation="MTPDTRQPGTGINIFIDDDPPLTGARIKVIGIGGGGSNAVNRMI
                     EAGIEGIEFLVANTDLQALKRSRAPIKIQLGSRLTKGLGAGADPGVGREAALEDTDKI
                     IEVLEGADMVFVTTGLGGGTGTGAAPIVASLATELDALTVAVVTKPFHFEGRRRMQQA
                     DQGLRELRECVDTVITIPNERLLHTVDRGVSLSDSFRVADDVLRQAVQGISDLITVPG
                     LINLDFADVKSIMQGMGMALMGAGRATGENRAIEATQQAISSPLLEEATIQGAKGVLI
                     KITGGPDLTLFEVNAASSIIRESADDDANIIFGAVIDEDLTDEMKITVIATGFDKESA
                     SLASAAATTVMPAPQP"
BASE COUNT          864 a         1050 c         1212 g          750 t
ORIGIN      
        1 cgtcgcgcgt gaaggaaagg tcgtggacat tccgggcgcc aatatccagt ggaactaccg
       61 ccacacgtca tttcaggaag gcgaattgtt gctgggagca acgctgcgtc tcaaacatgc
      121 cgatcccgaa cagatcaagg cgcgcatgga cgatgcgaag agtcgcaggc ttgcgacgca
      181 accgcatggc tcgcgttcgg caggctgctt ctttaaaaac cccccggcga gcacgactgg
      241 caccggcaag atcatcgacg agatggggat gaaaggtcag cgtcgcggca gtgcgattgt
      301 gtcgcccgtg catgcgaact tcatcgttac ggaaggtgag aacgcgcgcg ccgaagacgc
      361 cctcgcgttg gcggaagaga tccgcgaacg tgtgaagaga gaacagggga ttgaactcga
      421 gtacgaagtt gagttgtggc gtgcgaaccc agagccaccg acggcaacgg aaaacacgga
      481 tcagacgagg aaggaggagg agcgttgaga gaaacagttg ttccgcagaa agttggcaat
      541 cgcaccggta ttggcaaagc gcgttcgggc atgggccaac gtccggccag gcgcgctccg
      601 gccgagtcga ttggcgcgcg atttcgatct cttcttggtt acgttccaac cgcgcttcgg
      661 atcgccgcga ttgcgatcgc ttgcctcctc gtcgtggttg tttatcgcgc ggcggcgtcc
      721 gctagtttct ttcagatcaa aaaagtcgag acgaagggag cggctcgcgc ttccgttgaa
      781 gacattcaag cagtcgtgag gcgcgatgtg gcagcaaccg gcgtatggcg cgcaaacctt
      841 gatgagatca gccagcatct cgagcgcctg ccgtgggttc gcagcgcgat cgtgacgcgc
      901 gtgttgccgg atggaattcg cgtccgcatc actgagcgcc agccgcgggc agtagtgcga
      961 aactcgtccg gacgattcgt ttgggtagac gacgacgcag tttttctcgg cgaaatggcg
     1021 tcaacggatc agatgccgcc gtttttcctg cgtggctgga gtgaagaagc aggcgcacaa
     1081 gctgagaacc gccaacgtgt cgcgaagttt ctcgcgttgc aaaaggattg ggaggagcag
     1141 gggatcgcgg agcgtgtgag tgaagtgaat gtgatggacc ttcgcgacct gcgggtgcaa
     1201 cttgccggcg acgactcgca gatcgaagtg cgattaggcg gacaggacca gggcgcgcgt
     1261 ttgaagaaag cactgaccgt gctcgacgcg caacggcaaa cggcccgcgg gccactcatt
     1321 agttacatcg acttgagcca gggcaagaga gcaatcgttg gtttgactac cggcaaccaa
     1381 actgtcgctg aaggcacacc cccagcacag agatagtagg agcacagtag atggccaagc
     1441 gcacgcaaca cacaatcggt ctcgacattg ggaccagcaa ggttacgtgt attgccggtg
     1501 agcctggcga aggtgggttg ctcgaaatag tgggcatcgg tgaagcggat gctcgcggct
     1561 tgcgcaaggg agtcatcgtt aatcccgacg cggccgtcga tgcgatcaag aaagccgtcg
     1621 aagaagtgga gcgcatgagt ggtttggaag cggaggaagt aacaatcaat ctggcaggtt
     1681 cgcacattct cggcttcaat ggccaggcta tcgtggctgt ctccggtcgc gatcgcgaaa
     1741 tcgtcgcgga agacgtgcgg cgcgcgatcg attcggcgtg tgcaattcaa ctcccggccg
     1801 gtcgtgaaat cgtcgaccgg ttaccgcagg aattcattgt tgacgatcag gacggcatca
     1861 acgatccggt aggaatgatc ggcgcgcgac tcgcagtcaa agtccacatc gtcaccagtc
     1921 cggttactgc ccggcagaac gccatcaacg ccgtcaaccg cgccggcctg gtcgtggccg
     1981 acatggttct ggagcaactg gccgccgcgg aagcgtcgct aactgaagac gataaagagt
     2041 ttggcgccgc actcgtagac gtcggcgcag aaacaacggg actcattatt taccagcgcg
     2101 gcgctgtgca acacaccgct gtgtttcccc ttgggggttc gcattttact aacgacatcg
     2161 cttttggatt acgcacgcca atccccgagg ccgagaagat caaacgcacg gtcggctgtg
     2221 cgtgcagcag ttcgttgagt gagatggaac gcggcgaact agtcgacgtg ccttccgtcg
     2281 gagggcgccc gccccgtcag ctctcgcgac agattctttg cgacatcctt cagccgcgcg
     2341 cggaagaagt gttgatgcac gtcgcggacg agatcaaaga cgcaggttgg gaacgtcaac
     2401 tctcgagtgg cgttgtattg actggcggtg gcgcactgtt aagtggcatg actgagatcg
     2461 ccgagcaggt gtttgatgca ccggttcggc ttggatatcc cgaacgagat cgctttggcg
     2521 ggttgattga agacattcaa agtcctgcct gggccgcggc tgccggtctt gcgttactcg
     2581 cctatcgcac tcaagcagca gagatccgca ccggagtaag tcgacgcgga tcgacaggca
     2641 agctgacgca cttcgtatca cgatttaaaa atcgtttcgg tagtattttc tgacaacttg
     2701 ttgtatgctt gcgccccggt tgcacaagat attgtccgca atatatcgat gccgatggcg
     2761 ccaccagccc aacatagaag aaagcggcct tggaagccat cccgttttgg gagagaggaa
     2821 ctaactatga caccggacac tcgtcaaccc ggaaccggca ttaatatttt tatagacgac
     2881 gatccgccac tcaccggagc gcggatcaaa gtaatcggca tcggcggtgg cggcagtaac
     2941 gcagtcaacc gcatgatcga agcgggcatc gagggcatcg agtttctggt tgccaatacg
     3001 gatctacaag ccctcaagcg ttcgcgcgcg ccaatcaaga tccaactcgg cagcagattg
     3061 acgaaaggcc tcggcgccgg tgcagatccg ggtgtcggcc gcgaagccgc actggaagac
     3121 actgacaaga tcattgaagt gctcgaaggc gcggacatgg tctttgtaac gaccggtctc
     3181 ggtggtggaa caggaacggg cgcggcgccg atcgtcgctt cgctcgcgac cgagctcgac
     3241 gcgttgactg ttgccgtcgt cacgaaaccg tttcacttcg aaggacgccg gcgcatgcaa
     3301 caggccgatc agggattgcg cgagttgcgc gagtgcgtcg acaccgttat cacgattcca
     3361 aacgagcgac tgttgcacac cgtcgaccgc ggagtttcgt tgtccgactc cttcagagtt
     3421 gctgacgacg tgctgcgcca ggccgtgcaa ggcatcagcg atctaatcac cgtgcccgga
     3481 ttgatcaacc tcgacttcgc cgatgtgaag tcgatcatgc agggaatggg aatggctttg
     3541 atgggcgccg gtcgtgcgac cggtgagaat cgcgccatcg aagcgacgca gcaagcaatc
     3601 tcgtcgccgt tgctcgaaga agcgacgatt cagggcgcga aaggtgtttt gatcaagatc
     3661 actggtggac cagatctgac gttgtttgaa gtcaatgctg cttcgtcgat cattcgtgag
     3721 tctgccgacg acgatgcaaa catcatcttc ggcgccgtca tcgatgaaga tctcacggac
     3781 gagatgaaga tcacggtgat cgcgacgggc ttcgacaaag aatcagcgtc gctggcttcc
     3841 gctgcggcga ctacagtcat gcccgcgcct caaccg
//
LOCUS       DASTEY010000295         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1440180, whole genome shotgun sequence.
ACCESSION   DASTEY010000295 DASTEY010000000
VERSION     DASTEY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440180"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VFO72_05150"
     CDS             <1..1414
                     /locus_tag="VFO72_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9478706.1"
                     /translation="GLRLNISSWAGLLALQIMSATLLILVPALLMGMVMPLVLVWASN
                     HVKAVNRVGRSYAVNTIGAIAGAFIAGFVLIPTTSIRFTLLLAASLCVFVAAGAYEPA
                     QTGLDPAIKRAFAIGLGAPVLAIVLFILAPPMNLADLSIGAYDSLVRVLAQTREGTTD
                     EASGPDVHKLLMYREGPTATVSVRQDGDIVSMAINGRTNASDGIYDMPTQVMLGQLPM
                     LIAPRQSKGLIIGFATGVTVGSMLQSPIESVTCVELEPATVEGSRFFEHVNNRPLDDP
                     RTKFIVDDARTYLRVTPERYDIIVSEPSHPWIPGVANLFTQEFFELGRQRLSEQGIFV
                     QWVQIYQLSTESLRSVLATYHKVFPHVLVFRVAGSSRGKDLLLIGSNERLSLDGLSQR
                     LLDQRVAAELARVNIRSEADVRSWFVCDESRLGPAVAGARINTDDNMHIETTVPREAF
                     RPLMQTNAEWVQALAMPLPH"
     gene            complement(1423..>2384)
                     /gene="hemG"
                     /locus_tag="VFO72_05155"
     CDS             complement(1423..>2384)
                     /gene="hemG"
                     /locus_tag="VFO72_05155"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET9478707.1"
                     /translation="IADESLASFVRRRLGREALERMAQPMVGGIYTADPETLSLRATL
                     PRFLDMERDHRSLILAMLRKARSESGTSGARYSLFLSFDRGMQVLVQALGKAISGCEI
                     RLDTRVSTIERERGWRIKTDRAETINTDALCLAVPAYVASRLLANVNPALATQLNQIK
                     YASTATINFAYRRDAIPHPLDGFGFVVPFVEKRSLIACTFSSVKFPGRAPDDHVLLRA
                     FVGGALQPEMFALDEAEMLARVQNDLRKLLGITAKPLFAEVSKWQESMPQYEVGHLDL
                     VQAIESEMARLPNLSLAGNAYRGAGVPDCIRSGEAAAEQLLDS"
BASE COUNT          476 a          658 c          723 g          527 t
ORIGIN      
        1 cggattgcgc ctgaacatct cgtcatgggc cgggttgctc gcgttgcaga tcatgtcggc
       61 gacgctgctg atcctggtgc ctgcgttgct gatgggaatg gtcatgccgc ttgtgttggt
      121 ttgggcgagc aatcacgtta aagccgttaa ccgcgtggga cgaagttacg cggtaaacac
      181 catcggcgcg atcgcgggcg cgtttattgc cggttttgtg ttgattccca cgacgagcat
      241 tcggtttacg ttgttgttgg cggcgagttt gtgtgtattt gtggccgctg gtgcttacga
      301 acccgcgcaa acaggccttg atcccgcaat caaacgcgct tttgcaatcg gacttggagc
      361 gccagtgctg gcgatcgttc tttttatact cgcgccaccg atgaatctgg cggatctctc
      421 aatcggcgcc tacgacagtc tcgtgcgcgt actggcgcag acacgtgaag gcacgacgga
      481 cgaagcgagt ggaccagacg ttcacaaact gctgatgtat cgcgaaggtc caacagcgac
      541 ggtctccgtg cgtcaggatg gcgacatagt ttcgatggca attaacggcc gcaccaatgc
      601 gtcagatggc atctacgaca tgccgacgca ggtgatgttg ggccaactgc cgatgttgat
      661 cgcgccgcgg caaagcaaag gcttgatcat cgggtttgcc acgggagtga cagtcggttc
      721 gatgttgcag tcgccgatcg aatcggtaac gtgcgttgaa ctcgaacctg cgaccgtcga
      781 aggatcgcgg ttcttcgagc acgtcaacaa ccgtccgctt gacgatccgc gtacgaaatt
      841 catcgtcgac gacgcgagaa cttatttgcg cgtcacacca gagcgttacg acatcatcgt
      901 aagcgaaccg agtcatccat ggattccggg tgtggcgaat cttttcacgc aggagttttt
      961 cgagctgggc cgccagaggc tgagcgagca gggaatcttt gtgcagtggg ttcagatcta
     1021 tcagttatcg acagagagtt tgcgttcagt gctggcgacg tatcacaaag tgtttccgca
     1081 cgtgctcgta tttcgggtcg cgggatcaag ccggggcaag gatttgctcc tcattggcag
     1141 caacgaacgg ctgagtcttg acggcttgtc gcagcggctg ttggaccagc gagtggcggc
     1201 ggagttagcg cgagtgaata tccggtccga agcggatgtc agatcgtggt tcgtttgtga
     1261 tgagtcgcgc ctggggccgg ccgttgctgg ggcgcgaatt aacacggatg acaacatgca
     1321 cattgagacg accgtgccgc gcgaggcttt tcggccgctg atgcaaacga acgcggagtg
     1381 ggtgcaggcg ctggcgatgc ccttgccgca ctagcgagac tgctaagagt cgagcaattg
     1441 ctcggcggcc gcttcgccgc tgcggatgca gtcagggact ccggcgccgc gatacgcgtt
     1501 gccggcaagc gacagattcg gaagtcgggc catttcgctt tcgattgctt gtacgagatc
     1561 gagatgaccg acttcatatt gcggcatcga ttcttgccat ttcgagactt cggcgaagag
     1621 cggcttcgct gtgatgccca aaagtttgcg cagatcgttt tgtacgcggg ccaacatttc
     1681 agcttcgtcc agcgcaaaca tctccggctg cagggcgcca ccgacaaaag cgcggagcag
     1741 cacgtgatcg tcgggtgcgc gtccgggaaa cttcacgctg ctgaacgtgc acgcgatcaa
     1801 tgatcgcttt tccacgaacg gcaccacgaa accaaaacca tcgagtgggt gtgggatcgc
     1861 gtcacggcga tacgcgaaat tgatcgtggc ggtagaggcg tacttgattt ggttgagttg
     1921 cgtggcgagc gccgggttta cgtttgcgag cagtcgcgat gcgacgtagg cgggaaccgc
     1981 taaacagagc gcatctgtgt tgattgtttc tgcacgatcc gtcttaatcc gccaccctcg
     2041 ctcgcgttcg atcgtcgaaa ccctcgtatc caatcggatc tcacaccccg agatcgcctt
     2101 cccaagcgct tgcaccaaca cttgcatccc gcggtcgaac gaaagaaaca aactgtatcg
     2161 cgcgccgctt gtcccactct ccgatcgtgc tttgcgcagc attgccagga tcagactgcg
     2221 gtgatccctt tccatgtcga gaaaccgcgg cagcgttgca cgtaaactca gcgtctccgg
     2281 atcggctgtg taaatgccgc cgaccatcgg ttgggccatc cgctccagcg cttcgcgccc
     2341 caatcgccgc cgcacaaaac tcgcgagcga ttcgtccgca atac
//
LOCUS       DASTEY010000296         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1473083, whole genome shotgun sequence.
ACCESSION   DASTEY010000296 DASTEY010000000
VERSION     DASTEY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473083"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFO72_05160"
     CDS             <1..253
                     /locus_tag="VFO72_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="with 7S RNA and Srp19 binds to the signal sequence
                     of presecretory protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein Srp19"
                     /protein_id="HET9478708.1"
                     /translation="HETLLVVDAVTGQNGLEQARQFLKVAGVTGLVLTKLDGTAKGGI
                     AVAIAKELNLPIRYAGIGEKVDDLVVFDPHQYVNSLFGN"
     gene            253..1338
                     /gene="ribD"
                     /locus_tag="VFO72_05165"
     CDS             253..1338
                     /gene="ribD"
                     /locus_tag="VFO72_05165"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9478709.1"
                     /translation="MTETDRRMMARALELAKSGVGQVSPGPLVGCVIVSSEGELVGEG
                     FYIFEQLNHAETIALATAGEKARGGTAYVSLEPHAHHGRTPPCTDALIAAGIKRVVAP
                     IEDLNPKVSGKGFAHLRAAGLEVQTGLLAAEATQVNEAYLHYMRTGRPFVHLKFAASL
                     DGKIATRAGDSRWVTGAESRARVHELRHAYDAILAGAGTVLKDDPLLTDRSGLPRRRP
                     LVRVVLDDRVRLSPESKLATTTCEAPVLVFGRDATHRKMSGVELVNTDPADLKEVLAE
                     LGRRSLQSVLVEGGATVAGAFIDAGLVNKVTAFIAPKIVGGSNAPSAIGGKGIDQMAE
                     ALELERVEVIQRGRDIEVTGYPAGQTT"
     gene            1335..2141
                     /gene="rsmA"
                     /locus_tag="VFO72_05170"
     CDS             1335..2141
                     /gene="rsmA"
                     /locus_tag="VFO72_05170"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9478710.1"
                     /translation="MNAEQPFPSKRFGQHFLKDKRAIQRIIDALAPEAGETIVEIGPG
                     TGALTSELIQRAGRVVAVEFDKKLAPLLSEQFGSFPNFKLVMADALATDFCHEIKPAS
                     QARLVANLPYNISTAILQRLIEQRECLSEMVVMLQREVVERIMAPAGSSERGFLSVLI
                     EAYCETEKLFDVGPGAFRPPPKVWSSVMRLRFKPRTYTDLFWAAVSAGFSQKRKTILN
                     NLRHATGHLAEVLKRNGGASIVLCKAGVELNRRAETLTIEEWGRIVSAME"
     gene            complement(2144..3478)
                     /locus_tag="VFO72_05175"
     CDS             complement(2144..3478)
                     /locus_tag="VFO72_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9478711.1"
                     /translation="MTSLPPGPKGKLITGVMREFNRDTLGFITRLHRDYGDVVRSRFL
                     YVYAHFLYNPADIEALLTTNARSYRKAQSLRSPFFHRLVGNGLVTSEGGFWRRQRRLA
                     QPAFHRQRISSYGDIMVQYTKRAIAKWQNGEQRDIARDMTRLTLEIVVKTLFNSDVSN
                     EADHVGATLSEIVKPFASQATLKWIADNRLPTSGHRRYFNAVSEIDRIVFRIIAERRA
                     SGSDEGDLLSMLLQAQDEDGSQMSDAQLRDEVMTLFLAGHETTALALSWSWYLLATHP
                     EAESKFHAELEEVLGGREPDVSDLPKLKYTEMIAKEAMRLYPPAYAIGREAIEDTELG
                     GFRVPRKTQLFAFQWVTHRDPRFFERPDDFEPERWARESIERLPKFAYFPFGGGPRQC
                     IGNYFAMLEIVLLLATIGQRFKFSMLEGQPVEVLPVLSLRPKNGIKVRLVQR"
     gene            complement(3530..4135)
                     /locus_tag="VFO72_05180"
     CDS             complement(3530..4135)
                     /locus_tag="VFO72_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9478712.1"
                     /translation="MKPKFFATPEQFREWLEKNHASATEIEVGFHKKSSGKKSITYAE
                     ALDEALCFGWIDGVRHRLNETSYTTRFTPRRPRSIWSLVNLRHVERLQKEGRMRPAGL
                     EALARREPKRTGIYSFENSPRELLAEYERELRKNKPAWEYFQTYPPYLKKQVSFWVMS
                     AKKEETRLKRLRRLIESSAKGERIGVLPAKSAEGVGSKGRS"
     gene            complement(4148..>5552)
                     /locus_tag="VFO72_05185"
     CDS             complement(4148..>5552)
                     /locus_tag="VFO72_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9478713.1"
                     /translation="LVAVSPLSLPAQVRKRAAVTTPPILERNIRAELSFLASDAMQGR
                     GSGTVYERIAAEYVGSQFRQFGLEPAGDVDSSGNKGFVQRVSLETSRFTEPPTFTVTS
                     GSNTHKWKYGRDFLVSALRASRISGELQVIEPTGTPAKGAIVVIKLAETNHPEQRRNL
                     MMKAVGAGAVAVVLPETEANRKTRQAGDARLPNLPARNPGDQSGERQFAVIALPKDAM
                     ETLIGLPPGARAEFAGTIQRSEAGATWNAIGVLRGTDPSGEAILLSAHLDHLGVQESL
                     SGDNIFNGADDDASGCVAVLELARVLAARTKPRRTIYFICFGSEERGGYGSRYFISNS
                     PVPVEKIVANLNFEMLGRPDEKVPAGTLWLTGFERSTLGPELARQGAALVGDPHPEQN
                     FFQRSDNYTLALRGVVAHTVSSFGLHKDYHRPSDDISTIDFAFMTRSINSMVKPIQWL
                     SNSRFRPVWLPGQAPTR"
BASE COUNT         1165 a         1597 c         1598 g         1192 t
ORIGIN      
        1 gcacgaaacg cttttggtag tcgatgcggt cacgggtcag aacgggctcg aacaggcgcg
       61 gcagtttttg aaagtagcgg gcgtgacggg tcttgtgctg acgaagctcg acggcactgc
      121 gaaaggcggt attgccgtgg cgattgcgaa agagttgaac ctgccgatcc gctatgcggg
      181 gattggcgag aaggttgatg atctggtggt gtttgatcct catcaatacg tgaatagtct
      241 gtttggtaat tgatgaccga aactgatcgg cgcatgatgg cgcgagcgct ggagcttgcg
      301 aaaagcggcg tgggccaagt cagtcctgga ccactcgtcg ggtgcgtaat tgtcagctct
      361 gaaggcgagc tcgttggtga aggtttctat atctttgaac aactgaatca cgccgaaacg
      421 atcgcgctcg cgaccgccgg cgagaaagcg cgcggcggca ctgcttacgt ctcactcgag
      481 ccgcacgcgc atcacggccg cacgccgccg tgcaccgatg ccttgattgc ggcgggtatc
      541 aagcgcgtcg tcgcgccaat cgaagatctc aatcccaaag tttccgggaa aggctttgcg
      601 catctgcgtg cggcgggtct cgaagtgcag actggcctgc tcgccgcaga ggcgacgcag
      661 gtgaacgaag cgtaccttca ttacatgcgc actggccggc cgtttgtaca tctcaagttt
      721 gctgcttcgc tggatggaaa gatcgcaacg cgagcaggtg attcgcgttg ggtaacgggg
      781 gcggaatcgc gcgcgcgcgt gcatgaactg cgtcacgcgt acgatgcgat tctcgccggc
      841 gcgggaaccg ttttgaagga tgacccgctt ttgacggatc gctcgggttt gccgcgacgc
      901 aggccgttgg tcagggtagt gttagacgac agggttcgac tgtcgccgga atcaaagctg
      961 gcgactacga cctgtgaagc gccggtactt gtgttcggca gggacgcaac ccacaggaag
     1021 atgagtggcg tagagttggt gaacacggat ccagcggatt tgaaggaggt gttagctgag
     1081 cttggtcgtc gttcgcttca aagcgtcctg gtcgaaggcg gcgcaaccgt cgcgggcgcc
     1141 ttcatcgatg cgggcctggt gaacaaggta accgcgttca ttgcgccaaa gatcgtcggc
     1201 ggcagcaacg caccgagcgc catcggcggt aaaggaatcg accagatggc cgaagcgctc
     1261 gagctcgaac gcgtcgaagt aattcaacga ggccgagaca tagaagtcac cggttatccg
     1321 gcagggcaaa caacgtgaac gccgaacaac cttttccgtc caaacggttc ggccagcact
     1381 tcctcaaaga caagagagca atacaacgca tcatcgacgc actcgcgccc gaagccggcg
     1441 aaacaatcgt cgagattgga ccaggcactg gcgcgctcac gagcgaattg atccagcggg
     1501 caggcagagt tgttgctgtc gagtttgata agaaactggc gccgctgttg agcgagcagt
     1561 ttgggagttt tccaaacttt aaactcgtca tggcggacgc actggcgaca gatttctgcc
     1621 acgagattaa acccgcgagc caggcgcgcc tggttgcgaa tcttccgtac aacatctcca
     1681 ccgccatctt gcagcgactg atcgaacagc gcgagtgttt gagtgaaatg gtggtgatgt
     1741 tgcaacgtga agtcgtcgaa cgaatcatgg ctccggcagg atcgagcgaa cgcggttttc
     1801 tttcggtgct gatcgaagcg tattgcgaga cagagaagtt gtttgatgtt ggaccaggtg
     1861 cgttcaggcc gccgccaaag gtttggagta gcgtgatgcg gttgcgtttt aagccacgga
     1921 cttacacgga tttgttttgg gccgcggtaa gtgcgggatt ttcgcagaag cggaagacaa
     1981 ttcttaacaa cctgcggcat gcgacagggc acctggcgga agttctgaaa cgcaacggtg
     2041 gcgcgagcat tgtcttgtgt aaagcgggcg tggaattgaa ccgtcgcgcg gagacgttaa
     2101 caatcgagga gtggggtaga attgtcagcg cgatggagta ggttcaacgc tgaaccaatc
     2161 tcaccttgat gccatttttc ggccgcagcg aaagaaccgg aagaacttcc actggctggc
     2221 cctcaagcat tgaaaacttg aaccgctgtc cgatcgtggc tagcaacaac actatctcca
     2281 gcatcgcgaa atagttgccg atacactgtc gtggtccacc gccaaacgga aagtaggcaa
     2341 acttcggcag acgctcaatc gactccctcg cccaacgttc aggctcaaag tcatcaggac
     2401 gctcgaaaaa ccgcgggtcg cgatgagtga cccactgaaa cgcgaatagc tgggtttttc
     2461 gcggcacgcg gaaaccacca agctcagtgt cttcgatcgc ctcgcgaccg atggcgtacg
     2521 cgggcggata caaacgcatc gcttccttcg cgatcatctc cgtgtatttc agtttcggca
     2581 gatccgaaac atccggctcg cggccgccaa gcacctcctc caactccgcg tgaaacttgc
     2641 tctccgcctc cggatgtgtc gccagcaaat accagctcca cgacaacgca agcgccgtcg
     2701 tttcgtgacc ggcaagaaac agcgtcatca cttcatcgcg cagttgcgcg tcgctcatct
     2761 gactgccgtc ctcgtcctgc gcctgcaata gcatcgacag cagatcgcct tcatccgaac
     2821 cgctggcgcg ccgttcggca attatgcgaa acacgattcg atcgatctcg ctgacggcgt
     2881 tgaaataacg ccggtgaccg gaagtcggga gccgattgtc ggcgatccat ttcagtgtcg
     2941 cttgtgaagc aaacggcttc acgatctcgg acagtgtggc gccgacgtga tcggcttcgt
     3001 tggaaacgtc ggagttgaag agtgtcttga ccacgatttc cagcgtcaaa cgcgtcatgt
     3061 cgcgggcgat gtcgcgctgt tcgccgttct gccatttggc gattgcgcgc ttcgtgtact
     3121 gcaccatgat gtcgccgtaa gagctgatac gctgccggtg aaacgccggt tgggccagcc
     3181 gtcgttgccg gcgccagaaa cctccttcgc ttgtgacgag tccatttccg accagccggt
     3241 gaaagaaggg agaacgaagc gactgcgctt tgcgataact cctggcgtta gtggtgagga
     3301 gtgcttcgat gtcagccggg ttatagagaa agtgtgcgta gacgtaaaga aatctcgacc
     3361 gcactacgtc gccgtagtct cgatgcaagc gcgtgatgaa accgagcgtg tcgcgattga
     3421 actcgcgcat gacaccggtg atcaacttgc cctttggccc aggcggcagc gaggtcattg
     3481 cttgttcttc agcacattca atttcttcgt ctcacgcttg cggctcggat caacttcgcc
     3541 cttttgatcc tacgccttcg gcgctcttcg ccggaagcac gccgatgcgc tcgcctttcg
     3601 ccgaactctc aatcaaccgc cgcagccgct tcaaacgcgt ctcttccttt ttggcactca
     3661 tcacccagaa cgaaacctgc tttttcaagt atggcgggta cgtctgaaag tactcccacg
     3721 cgggtttgtt cttccgcaat tctctttcat actcagccaa tagttccctt ggcgaattct
     3781 cgaacgagta aatcccggtt cgtttgggct cgcgcctggc aagagcctcg agtcccgcgg
     3841 gccgcatgcg cccctctttc tgcagacgct cgacgtgcct gagattaacc agactccaga
     3901 tgctccgcgg cctgcgcggt gtgaagcgcg tcgtataact cgtctcattg agtctgtggc
     3961 gcacgccgtc gatccaaccg aaacacagcg cctcatccag cgcttcggcg taagtaatac
     4021 tcttctttcc ggaacttttc ttatggaagc cgacctcgat ttcggtagcg ctcgcgtggt
     4081 tcttctccag ccattcgcga aactgctccg gcgtagcaaa aaacttcggt ttcatggcca
     4141 tcatcgctta ccgagttggc gcttgacccg gcagccagac cggacgaaag cgcgaattgc
     4201 tgagccattg aatcggcttc accatcgagt tgatcgaacg cgtcatgaac gcaaaatcga
     4261 tcgtgcttat gtcgtcactt ggccggtgat aatccttgtg caagccgaaa ctcgagactg
     4321 tgtgcgcgac cacgccgcgc aacgcgagcg tgtagttgtc tgagcgttga aagaagttct
     4381 gctccggatg cggatcgccg accagcgctg ctccctgtcg agcgagttcc ggtccaagcg
     4441 tcgaacgctc gaaaccagtc aaccaaagcg tgcccgccgg caccttctca tcaggccgcc
     4501 ccagcatttc aaagttcaag ttggcaacaa ttttttcgac cggcacagga gaattcgaaa
     4561 tgaagtagcg agatccgtaa ccgccgcgtt cctcgctccc gaagcagatg aagtagatcg
     4621 tgcggcgcgg ttttgttctc gcggcaagga cacgtgccag ttccagcacc gcgacacatc
     4681 ctgaagcatc gtcatcagcg ccgttgaaaa tgttgtcacc gctcagtgac tcctgaacac
     4741 cgagatggtc caggtgcgcg ctcaacagga tcgcctcgcc gcttggatcc gtgccgcgca
     4801 acactccgat agcattccag gtcgctcccg cttccgaccg ttgaatagtg ccggcaaact
     4861 cggctcgtgc gcccggaggc aatccgatga gcgtctccat cgcgtcttta ggaagagcaa
     4921 taaccgcaaa ctgtctttcg ccactctggt cgccgggatt tctcgcaggc aaatttggca
     4981 acctcgcgtc acccgcctgg cgcgtcttgc gattggcttc tgtttccggc aacactacgg
     5041 cgacagctcc cgccccaacc gctttcatca tcaggttgcg tcgctgctcg ggatgatttg
     5101 tttccgccaa cttaatcacc acgatcgcgc ctttcgcagg cgtccctgta ggctcaatca
     5161 cctggagttc gccgctgatc cgcgatgcgc gcaaagccga aacgagaaag tcgcggccgt
     5221 atttccactt gtgcgtgttg cttcccgacg tcaccgtgaa ggtcggcggt tcggtgaatc
     5281 tcgaggtctc gagcgatacg cgctgtacga aacctttgtt acccgaactg tctacatcgc
     5341 ccgccggttc gaggccgaac tgtcgaaact gcgagccgac gtactccgcc gcgatgcgtt
     5401 cgtaaacggt gccactgccg cgaccctgca ttgcgtcgct cgccagaaaa ctcaactccg
     5461 cgcgaatgtt tcgttcgagt atcggcggcg ttgtgaccgc cgctcttttt ctgacctgag
     5521 ccggcagcga aaggggactg actgcgacta gc
//
LOCUS       DASTEY010000297         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1192961, whole genome shotgun sequence.
ACCESSION   DASTEY010000297 DASTEY010000000
VERSION     DASTEY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192961"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..411)
                     /locus_tag="VFO72_05190"
     CDS             complement(43..411)
                     /locus_tag="VFO72_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET9478714.1"
                     /translation="MDKRRTKIFRDKLLDRREGLVGQVQAAELYSRERDAEATQDPAD
                     MAANAYTKELLVSMSDNDRQLLNLIDEALERIETSGYGKCIHCGEALPEKRLEAVPWA
                     RHCVRCQDLQERGLLGDDED"
     gene            complement(460..918)
                     /locus_tag="VFO72_05195"
     CDS             complement(460..918)
                     /locus_tag="VFO72_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET9478715.1"
                     /translation="MRFRIIWPGKTRDARLRALIEDYAERLSHFVRCEVTELKEPGRT
                     DKTGIDKETKRISDALRPGSLTVLLDPEGSEWTSEELAAQVRSWEGNGVKEVAFVVGG
                     PNGLAPELVSRADKRWSLSRLTLTHEMTRVLLLEQLYRAFTIIHGLPYQK"
     gene            complement(934..1866)
                     /locus_tag="VFO72_05200"
     CDS             complement(934..1866)
                     /locus_tag="VFO72_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9478716.1"
                     /translation="MIELFARSPGMREAVGLAERVSSTDANVWITGESGAGKDVLASF
                     IHWKSPRATQPLVKIDCATLPGDLLEAELFGYERGAFTGASEAKPGRFEAAQKGTLVL
                     DEIAHLSIGAQAKLLRVIETHEFERLGGRKTIKIDSRLIALTNVDLGDAVKAGRFRED
                     LFYRLDVIHIRVPPLRERKEDLSKLLQYFLKHYSAKHGRKVERVSAPALALLQAYDYP
                     GNARELANIVERAVIVANGKGLEEHDLPAGLSAAVSARRQQEKPPSLAELEAAYIAEV
                     LARTKGNKTECARILGISRKNLYEKIARYDLSHR"
     gene            complement(1863..2339)
                     /gene="rsfS"
                     /locus_tag="VFO72_05205"
     CDS             complement(1863..2339)
                     /gene="rsfS"
                     /locus_tag="VFO72_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9478717.1"
                     /translation="MKQNSTVKEQSLNPQPSARTQAQSVACTGGDELDERVCTALHAA
                     GEKKAIDPVVLDLREIASFTDYFVIVSGANERQVQAISDEVYETLKKAGEPAARVEGY
                     NTAEWILLDYGDFVVHVFEQKARRFYDLERLWRESKRVALPPELSGESSSSLRKES"
     gene            complement(2317..>2585)
                     /locus_tag="VFO72_05210"
     CDS             complement(2317..>2585)
                     /locus_tag="VFO72_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478718.1"
                     /translation="RDWEQLLKMTNHIVVTRPGYDVSPAPPGLDARIFFTDAVKKDVS
                     ATDIRRLASAGRYDELTTLVPQSVATYIRKYGIYRDSNEAKLNS"
BASE COUNT          525 a          830 c          645 g          585 t
ORIGIN      
        1 gtttcgagga ggccgcgatt atctcacaac gctcagacgc gtctaatctt cgtcgtcacc
       61 taaaaggcca cgctcttgca ggtcctggca acgaacacag tgtcgcgccc acggcactgc
      121 ttcgagtctc ttctcgggaa gtgcttcgcc gcagtgaatg cacttgccgt atcccgaagt
      181 ttcgattctt tccagcgctt cgtcgatcag attcagcaac tgccgatcgt tgtcggacat
      241 cgacaccaac agttctttgg tgtaagcgtt tgcggccatg tcggccggat cctgcgtggc
      301 ttcggcgtcg cgctcgcgac tgtaaagttc cgccgcctgc acctgaccaa ccagtccctc
      361 gcgacggtcc aataacttat cgcggaaaat cttagtgcgt cgtttatcca ttagtgctaa
      421 tcctccaccc tacttgataa atgccgggat tgccgatagt catttttgat atggcaagcc
      481 gtgaataatc gtaaacgccc gatacaactg ttcgagcagg agcacgcgcg tcatctcgtg
      541 tgtcagcgtc agccgcgaaa gcgaccagcg cttgtccgct cgcgatacca gctccggcgc
      601 caggccgttg ggtccgccga cgacgaacgc gacctctttg acgccattcc cttcccaact
      661 cctgacttga gccgccagtt cctcggacgt ccactccgaa ccttccggat ccagcaacac
      721 tgtcaacgac ccgggacgaa gcgcgtctga gatgcgcttg gtctccttat cgatgcccgt
      781 tttgtctgta cgccccggct ccttcaactc cgtcacttca caacgtacaa agtgcgaaag
      841 ccgttcagcg tagtcttcaa tcagtgcgcg cagccttgcg tctcgagtct tgcctggcca
      901 aatgatccga aagcgcatct gtgtccgtcc gcgtcatctg tggctcaggt cgtagcgagc
      961 gatcttctcg taaaggttct tacggctgat gccgaggatt cgcgcgcact ccgtcttgtt
     1021 tcccttcgtc cttgcgagaa cttcggcaat gtaagccgcc tccaactcag ccaggctcgg
     1081 cggcttctcc tgttggcgcc gcgcactcac tgccgcgctc agacccgccg gcagatcatg
     1141 ctcttccagc ccctttccat tcgcaacaat caccgcacgc tcgacaatgt tcgccaactc
     1201 gcgagcgttg cccgggtagt catacgcctg gagcagagcc aatgcaggcg ccgagacgcg
     1261 ttcgaccttg cgcccgtgct tggccgagta gtgcttcaag aagtactgaa gcagttttga
     1321 aagatcctct ttgcgttctc tcaacggcgg cacgcgaatg tgaatcacat ccaaccggta
     1381 aaaaaggtct tcacgaaagc gtccggcctt tactgcgtcg ccgagatcga cgttagtcag
     1441 agcgatcaaa cgcgagtcga ttttgatcgt tttgcgacca cccagacgct caaactcgtg
     1501 cgtttcgatc acacgcaaca acttcgcttg cgcaccaatc gaaaggtgcg cgatctcgtc
     1561 cagaacaagc gttcccttct gtgccgcttc aaaccgtccg ggctttgctt cgctcgctcc
     1621 cgtaaacgct ccgcgctcgt agccaaagag ctcggcttca agcaggtcgc cagggagcgt
     1681 tgcgcaatca atcttgacca acggttgagt agcgcgcggc gacttccagt gaatgaaaga
     1741 ggcgagcaca tccttccctg cgccggactc accggtaatc cacacattcg catccgttga
     1801 actgacgcgc tccgcgagac caaccgcctc gcgcattccg ggagagcgcg caaacagttc
     1861 gatcacgatt cttttctcaa agaactggaa ctctcgccgc tcaattcagg cggcaacgca
     1921 acccgtttcg actcgcgcca caaacgttcg agatcgtaaa accgccgcgc cttttgctcg
     1981 aacacatgga ccacgaagtc gccgtagtcc aacagtatcc actccgccgt gttgtaacct
     2041 tcaacgcgcg cggccggctc gccggccttc ttgagtgttt cgtaaacttc atccgaaatg
     2101 gcttgcacct gccgctcgtt tgcgccactg actattacaa agtaatcggt gaagctcgcg
     2161 atctcgcgca ggtccaacac gaccggatcg atcgccttct tctcgccggc agcatgaagc
     2221 gcggtacaca cgcgctcgtc gagttcgtcg ccgccggtgc aagcgacaga ctgagcttga
     2281 gtgcgcgcac tcggttgagg attcaacgac tgttctttaa ctgttgagtt ttgcttcatt
     2341 cgaatctcga taaatcccgt acttcctgat gtaagtcgct accgattgag gtactaacgt
     2401 cgtcaactca tcgtagcgcc ctgcactcgc aagccggcga atgtccgtcg ccgaaacgtc
     2461 tttcttcacc gcgtccgtga aaaatatccg cgcgtccaac cccggcggcg caggtgaaac
     2521 gtcataaccg ggtctcgtga cgacgatgtg attcgtcatc ttcaagagct gttcccaatc
     2581 gcgcc
//
LOCUS       DASTEY010000298         5057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1489711, whole genome shotgun sequence.
ACCESSION   DASTEY010000298 DASTEY010000000
VERSION     DASTEY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489711"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFO72_05215"
     CDS             <1..313
                     /locus_tag="VFO72_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9478719.1"
                     /translation="LLGRNPWGTSMFTGIPANGTYPRDVHLFPNALLKRMVRGGLIDG
                     PVYKKVFQSLRGVFIREPDPNAPFQDERAVYHDDINDYSTNEPTMDGTASAILLWAVR
                     A"
     gene            complement(358..1326)
                     /locus_tag="VFO72_05220"
     CDS             complement(358..1326)
                     /locus_tag="VFO72_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET9478720.1"
                     /translation="MKAIRVSQTGGPEALVFVDLPAPKPKPNEALVQIKAAGVNFIDV
                     YFREGRYPAQLPFVNGQEAAGVVTEVGGEVTTFQAGDRVAYTGALGSYAEYAAVPASR
                     LVKIPDELSFEQAAAVMLQGMTAHYLVHSTYKLRAGETALIHAAAGGVGLLLVQMAKK
                     IGARVIGTAGTQEKAQLARDAGADECIVYTEIDFETETRRLTNDKGVKVVYDGVGKAT
                     FDKDLNVLVPRGYLVLFGGASGAVPPFDLIKLSQKGSLYVTRPTLGHYTAAREELEWR
                     ANDVLQWIAAGELKLRLHKTYPLAEAAQAHRDLEGRKTTGKLLLVL"
     gene            complement(1392..2528)
                     /locus_tag="VFO72_05225"
     CDS             complement(1392..2528)
                     /locus_tag="VFO72_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9478721.1"
                     /translation="MVRIAIIGGGIGGLTVALALEEFGFEAEVYEQAPELLDVGAAIA
                     IWPNAMRVLERLGLADKILSHAGTMKEIRWLDQRGFLLNRLSIPRSVALHRADLQSTL
                     LHAVKTDSIHLGHSLTAYEQHSDKVIATFANGHTIEAEVLIGADGIHSRCRAQLLGDG
                     QPNFRGYNVWRGISSVSPETLPPATAIEIPGRGKRFGIGPVGHGRVGWWAAANAASQQ
                     PTVNLDPQNELLTLFKDWYRPALELIASTAPDNILTTAAFDREPTRVWGHKRMTLLGD
                     AIHPTTPNLGQGGCLAMEDSMVLARCFQQYGATEEALRKYESCRYTRTAAITKYSRLY
                     GSIGQWKNPLAHGLTTLLISLAPESVLQRLLQTVFDYDATTVPV"
     gene            complement(2542..3453)
                     /locus_tag="VFO72_05230"
     CDS             complement(2542..3453)
                     /locus_tag="VFO72_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9478722.1"
                     /translation="MKVAITGGAGFIGSHLATRLSSEGHEVLRLGRRSTAEVANASLD
                     DVDQLAHAFTGCQAVAHCAGINRETREQTYARVHIEGTRNVVDAARRAGVEKIVLMSF
                     LRARPDCGSAYHESKWAAEEIVRNSGLDYTIIKAGVVYGRGDHMLDHLSHAFHTFPIF
                     ALVGFEDKTVRPLAIEDLVQVMRAALIDRRLKQQTIALLGPEEIYLSEAVRRVAEVVN
                     KQPLMFPLPVWCHELMARVFELTMKVPLVSLAQVRILSEGVVEPSTPVAALPYDLLPT
                     RRFSFEQIRNGLPHPAPFCVGNLRLCS"
     gene            complement(3465..3653)
                     /locus_tag="VFO72_05235"
     CDS             complement(3465..3653)
                     /locus_tag="VFO72_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478723.1"
                     /translation="MTNVTSIQQSMRDTRVWEMDPVVRYILLGYLAAWAVFLLTRWHQ
                     TDVALSTIKEELRYYLID"
     gene            complement(3650..4360)
                     /locus_tag="VFO72_05240"
     CDS             complement(3650..4360)
                     /locus_tag="VFO72_05240"
                     /inference="COORDINATES: protein motif:HMM:NF036524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9478724.1"
                     /translation="MRKKKAAKTLKGDQTKARILETALEIFRERGYEETTMRAIAEKA
                     GVSLGNAYYYFSSKEYLIQAFYQRLHEEHLVVALPALEKHDTLKARLLTVMRLKIDVM
                     KPYHQFAGVLFKTAAHPESPLNPFSDESDPVREASIELFAKVLDGTKARIPKDLKAEL
                     PYLLWVYQMGIVLFWIHDPSRNHRRTYRLVEHTVDLLDKLIHLASNPFMRPLRKQALR
                     LVEEIREALPDDDEKEEP"
     gene            complement(4378..4743)
                     /locus_tag="VFO72_05245"
     CDS             complement(4378..4743)
                     /locus_tag="VFO72_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HET9478725.1"
                     /translation="MFALTVLYDPECGLCRRAHEWLAEQSKLVELTFVPCASDEARQR
                     FPQLNHDLTRKDLTVIGDDGAVYFGPKGWLMVLWALTRYREWSYRLASPELLPTTKRV
                     VSAISQNRYQISRVAGLGR"
     gene            complement(4749..>5057)
                     /locus_tag="VFO72_05250"
     CDS             complement(4749..>5057)
                     /locus_tag="VFO72_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478726.1"
                     /translation="VFLVDVFHGNEALADSVNHLLVVGFYLINLGYVSMALKLGYAIN
                     NTQEGIEALSVKIGMVLIVLGGMHFFNLFVFSRMRRRSNHQQHPPVGPDAFTTVQQEA
                     "
BASE COUNT         1063 a         1473 c         1417 g         1104 t
ORIGIN      
        1 gctgctgggc cgaaacccgt ggggcacgtc aatgttcacc ggcattccgg caaacggaac
       61 ttacccgcgg gacgttcatc tgtttccgaa cgcattgttg aagcgaatgg tacgtggtgg
      121 gctgatcgac ggtccggttt ataagaaggt ctttcagagt ttgcgcggtg tcttcatccg
      181 cgagcccgac ccaaacgcgc cgtttcaaga cgaacgtgcc gtctaccacg acgatatcaa
      241 cgactattcg accaacgaac ccacgatgga cggaacggcg tcggcgatcc tgttgtgggc
      301 agttagagct tagagctttg tacgttgttc tttgtaagaa agaccaagaa gaaaagctca
      361 cagaaccagc agaagctttc cggtcgtctt tctaccttcc agatcgcggt gtgcttgtgc
      421 tgcctcggcg agtgggtagg tcttgtgaag gcgcagcttc aactctcccg ccgcgatcca
      481 ttggagaaca tcgttagcac gccattcgag ctcttcacgc gcggcggtgt agtggcccaa
      541 cgttggacgc gtaacgtaaa gcgatccctt ctgcgagagc ttgatcaaat cgaacggcgg
      601 caccgcgcca ctcgcgccgc caaacaacac cagatacccg cgcggcacga gcacgttgag
      661 gtccttgtcg aaggttgcct tgccgacgcc gtcgtaaaca accttcacac ctttgtcgtt
      721 tgttagccgt cgcgtttccg tttcgaaatc gatttccgta tagacgatgc attcgtccgc
      781 gccggcgtcg cgcgcgagtt gcgccttctc ctgcgttccg gctgtgccga tcacacgggc
      841 gccgatcttc tttgccattt ggaccagtaa caatccgacg ccacctgccg ccgcgtgtat
      901 caacgctgtt tcgccggccc gaagtttgta ggtgctgtgc accaggtaat gcgcggtcat
      961 gccctgaagc atcaccgccg cggcctgctc gaaactcaat tcatccggga tctttacgag
     1021 acgactcgcc ggcaccgctg catattcagc ataacttccg agcgcgcccg tataagcaac
     1081 gcggtcgccc gcctgaaacg ttgtcacttc accaccaact tcagtcacaa ctcccgcagc
     1141 ttcctgaccg ttgacgaatg gaagctgcgc gggatagcga ccttcgcgaa agtaaacgtc
     1201 gatgaagttc acgccggccg ctttgatctg cacgagcgcc tcgttcggct tcggttttgg
     1261 cgccggcaag tctacaaaaa cgagcgcttc cggcccgccc gtttgggaca ctcgaatagc
     1321 tttcatgagc cacagattaa cacggaagac acagatgatt gccagtgccg tccgtggccg
     1381 gcggcaacgc cttaaacagg cactgtagtg gcgtcgtagt cgaagacggt ttgcagtaag
     1441 cgttgtagca cggattcggg agcgagcgag atgaggagtg tcgtaagtcc gtgggccagt
     1501 gggttcttcc actggcctat gctgccgtag agccgggagt attttgtgat ggctgccgtt
     1561 cgtgtgtaac ggcagctttc gtattttcgc aacgcttcct cggtggcgcc gtactgctga
     1621 aaacatctcg ccagcaccat cgaatcttcc attgcgaggc aaccaccctg acccaggttg
     1681 ggcgtggtgg gatgaatcgc gtcgcctaac aacgtcattc gcttgtggcc ccagacgcgt
     1741 gtcggttcgc gatcgaatgc ggcagtcgtc agaatattat cgggtgcagt ggaagcgatc
     1801 agttccaagg ccgggcgata ccagtctttg aacaaggtca gtagctcgtt ttgtgggtca
     1861 aggttcactg taggctgctg cgaggcggcg ttcgcagcgg cccaccaacc tactctgcca
     1921 tgacccactg gtccaatacc gaaacgtttg ccgcgcccag gaatctcgat tgctgttgcc
     1981 ggcggtagtg tctccggaga cacggatgag ataccacgcc aaacgttgta gccgcgaaaa
     2041 ttgggctggc catcgccgag caactgcgca cggcagcgcg agtgaatgcc gtctgccccg
     2101 atcagaactt ccgcttcgat cgtgtggccg tttgcaaacg tcgcgattac tttgtcgctg
     2161 tgttgctcgt aagcagtcag actgtggccc agatggattg aatcggtttt tacggcgtgc
     2221 agcagcgtgg attggagatc ggcgcggtga agtgccactg acctcggtat ggagagccga
     2281 ttgagcaaaa aaccgcgctg gtccaaccag cgaatctctt tcatcgtgcc tgcgtgcgac
     2341 aggatcttat cggcgagacc gagtcgctcc agtacgcgca tcgcgttggg ccaaatagcg
     2401 atcgccgcgc caacatcgag caactccggc gcctgctcgt aaacctctgc ttcaaaccca
     2461 aactcttcga gggcgagtgc gacagttaga ccaccaatgc caccgccgat aatcgcgatg
     2521 cggaccatcc gcgagagagt atcacgagca cagtcgtaaa ttgcctacgc aaaacggcgc
     2581 cggatgcggc aggccattgc gaatctgctc gaaactgaat cttcgtgtcg gcaacaggtc
     2641 gtaaggaagc gccgcgactg gcgtagacgg ctcgaccacg ccttccgaaa gaatccgcac
     2701 ctgggccagc gaaacaagcg gcaccttcat cgtcagttca aacactcgcg ccatcagttc
     2761 gtgacaccag acaggcaacg gaaacatcaa tggctgtttg ttgacgactt cggcgacacg
     2821 ccgtacggct tcgctcaaat agatctcttc cgggccaagc aaagcgatcg tttgctgttt
     2881 caagcgccga tcgatcaacg ccgctcgcat gacctgcact aggtcttcaa ttgccagcgg
     2941 ccgcacagtt ttgtcctcaa agccgaccag cgcgaagata ggaaaggtgt gaaacgcgtg
     3001 gctcagatga tcgagcatgt gatcgccgcg gccataaacc actccggctt tgatgatcgt
     3061 gtagtccaga ccggagttgc gcacgatctc ttccgctgcc catttcgact cgtgatacgc
     3121 agaaccgcag tcgggccgcg cgcggagaaa actcatcagc acgatctttt cgactcctgc
     3181 gcgccgtgcg gcgtcgacta cgtttcgcgt gccttcgatg tgaactcgtg cgtaagtctg
     3241 ttcacgagtt tctcgattga tacccgcgca atgcgcgact gcctggcagc cggtgaaggc
     3301 atgggccaac tgatcgacgt catctagcga cgcgttcgct acctccgcgg tggagcggcg
     3361 cccgagtcgc aaaacttcat gtccctcgct gctgagccgc gtagccaaat gactgccgat
     3421 gaaaccagcc ccgcccgtaa ttgcgacttt catggctctc gctcctaatc gatcaggtaa
     3481 taccgtagtt cttccttgat ggttgacagt gccacgtctg tttgatgcca gcgcgtcagc
     3541 agaaacacgg cccacgcagc cagatatcca agcaagatgt agcgaactac tgggtccatc
     3601 tcccacacgc gcgtgtcgcg catcgattgc tgaattgagg tgacgtttgt catggctcct
     3661 ccttttcgtc gtcatcgggc agcgcctcgc gaatctcttc caccaaacgt aacgcctgct
     3721 tgcgcagggg tcgcataaat gggttgctcg ccagatgaat caatttgtcg agcaggtcaa
     3781 cggtgtgctc gaccagacga taggtgcggc ggtggttgcg tgacggatcg tgtatccaga
     3841 acaacacgat tcccatctga tagacccaga gcaaatacgg tagttctgct ttcagatctt
     3901 ttggaatacg ggccttcgtt ccgtcgagaa ctttcgcaaa cagttcaatg ctcgcttctc
     3961 taaccggatc ggattcgtct gaaaacggat tcaaagggct ctccggatgc gcagcggttt
     4021 tgaaaagcac gcccgcgaac tgatgatacg gtttcatgac gtcgatcttc agccgcatca
     4081 ccgtcaacag gcgtgctttc aaagtgtcgt gcttttccaa agcaggcagc gctacgacga
     4141 ggtgctcttc gtgcagacgt tggtagaacg cctggatcag atactccttc gagctgaagt
     4201 aatagtaggc attcccgagc gacacacctg ccttctcggc aatggcgcgc atcgttgttt
     4261 cctcgtatcc gcgctcccga aatatctcga gggccgtttc cagaatccgg gccttcgtct
     4321 gatcgccttt cagcgtcttc gccgccttct ttttacgcat cgccctcatt agtttggtca
     4381 cctgcctaga ccagcaactc tgctgatctg gtatcgattc tgtgagatcg ccgagacgac
     4441 ccgcttggtc gtcggcaaca gttcgggact ggcgagccgg taggaccact cgcgataacg
     4501 agtaagtgcc cacagcacca tcagccaacc ctttggtccg aagtagacag cgccgtcgtc
     4561 gccgatcacg gtcaggtcct tcctggtcag atcgtgattc agttggggaa agcgttggcg
     4621 cgcttcatcc gacgcgcacg gtacaaaagt gagttcgacg agtttcgact gctccgcgag
     4681 ccactcgtgt gcgcggcgac acaaaccgca ttcagggtcg taaagcacag tcagggcgaa
     4741 catggctgtc acgcctcctg ctggacggta gtgaatgcgt ccggtccgac gggtggatgc
     4801 tgctgatggt tgctgcgccg gcgcatgcgg ctgaagacga agagattgaa gaagtgcatg
     4861 ccgccgagca cgatcagcac cattccaatc ttgaccgaca gggcttcgat accttcctgc
     4921 gtgttgttga tggcgtagcc gagcttcaac gccatgctga cgtacccgag gttaatcagg
     4981 tagaagccga cgacgagcag gtgattgaca gagtcggcga gggcttcatt gccgtggaag
     5041 acgtcgacga gaaagac
//
LOCUS       DASTEY010000299         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_632869, whole genome shotgun sequence.
ACCESSION   DASTEY010000299 DASTEY010000000
VERSION     DASTEY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632869"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1424)
                     /locus_tag="VFO72_05255"
     CDS             complement(135..1424)
                     /locus_tag="VFO72_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478727.1"
                     /translation="MQRLIGESFLVFAGIALLTVALLSLVPATRAQKDLQRVGTIAYV
                     RGSTEIRVINADGTNDRRVWTHPDAKRELGIYDVAWRPDGQELVFSSGHAAASSLYHA
                     DLYGIRPDGTGFRKITNSPDRAEFARYPKGSVSVTLRNDQPFYKQSQASSGVFIVYVA
                     GADEPQQITLPPGSSKTVVFKNVADFGKMAQAIVAIWGQYRWFTTGVDVRAAQTIKAP
                     VFSVSGDGIELLGAFRPVWRGDGSRVSYRSGVCTLNSVSATPVPGNYDYNPLFAGKNP
                     LGTCAWDWGPTSATANQLIYSENGSDDSGIFRITEGEKHPGDKLTSYSNIPYQILFDL
                     RWLPDGSGFLFSNQTLMRDSANIFRYDFATRRTTQITRLANEFTGNFSISPDGKWIVF
                     ERSKSLDENRAIDLWIAAIDGGNPRLLVRDAFSPSWR"
     gene            complement(1412..>2581)
                     /locus_tag="VFO72_05260"
     CDS             complement(1412..>2581)
                     /locus_tag="VFO72_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478728.1"
                     /translation="ATLKIDKSAWERLNCLRPIFSAAGLDLEFPKSGPLADTTVAWSL
                     VLGGDSRGALGTLIDLVSGEDDRDDIVYLKPKDGAHSMPNKQTANGDGVSEIYVIGAP
                     QDEDLSKRKLFEVYKAAGVTVGVQIKPMKIKDAQAAASTLGDIVGNVISFLTGDAIGG
                     GVGVAMETMYRSNWYSAKPFYFPVKDWEPCKGQWVGTITYTASLKEVGSAENNVNTST
                     WNDEEHYDSEIQISGKKTNLGAPLATVKATASQKRERTSTGKGVCYGTSTQIQQLKGS
                     DTVVTSGWSVSVNPRTREYSVSPPTIVVNGSGTYSIVSEVKGSCRNPFNKGIRQSQPQ
                     TGAKLTAEGPTLYGKGRIDPNNPDEISGADTVTIPTLKGGERKATITWSLRRCKD"
BASE COUNT          562 a          732 c          690 g          597 t
ORIGIN      
        1 aattcgccac gcacaatggt cgcaggaccg cgaacaagac aacctgacca ccgactctgc
       61 gcgccgcttt acccaggcgg gcgtcgacct tatcgtggat cattcatgag gaaccatcac
      121 tgattccgct gaggctaacg ccatgaaggc gagaaggcgt cgcgcacgag aagacgggga
      181 tttccgccat cgatggctgc aatccacaga tcgatcgccc ggttttcgtc taacgacttc
      241 gagcgttcga acacgatcca ctttccatcc ggtgagatac tgaagtttcc ggtgaactcg
      301 tttgccagcc tggtgatctg agtagttctg cgagtggcga agtcgtaacg gaagatgttg
      361 gctgaatcgc gcatcaaggt ttgattagag aacaaaaaac ccgagccgtc ggggagccaa
      421 cgcaggtcaa acagtatttg atagggaatg ttggagtagc tcgtgagctt gtcgccggga
      481 tgtttttcgc cttcggtaat gcgaaagatg ccggagtcgt ctgatccgtt ttcagaatag
      541 atgagttgat tcgccgtcgc agacgtgggg ccccagtccc aggcgcaggt gccgagagga
      601 ttttttccgg caaacagcgg attgtaatcg tagttgccag gcaccggcgt agccgacacg
      661 ctattcagcg tgcagacgcc tgaacgataa ctcacacgtg atccatcgcc gcgccacacg
      721 ggccgaaacg ctcccagcag ctcaatgccg tcacccgaaa cactaaacac cggcgctttg
      781 atcgtttgag ccgcgcgcac gtcaacgccc gtcgtaaacc aacgatactg gccccagatc
      841 gcgacgatcg cctgggccat cttgccgaaa tccgcgacgt tcttaaaaac aaccgtcttg
      901 cttgaacccg gtggcaatgt gatctgctgc ggctcgtctg caccggcaac gtaaacgata
      961 aacacgcccg aactcgcttg agactgtttg taaaacggct ggtcgttgcg cagggtgacg
     1021 ctgactgaac cttttgggta acgcgcgaac tcagcgcgat cgggtgagtt tgttatcttg
     1081 cggaagcccg tgccgtcagg acgaatgccg taaaggtctg catgataaag cgaagacgca
     1141 gcggcatgac cgctcgagaa gacaagttcc tgtccatccg gccgccacgc gacgtcgtaa
     1201 attcccagtt ccctttttgc atccggatgc gtccagacac ggcgatcgtt tgtgccgtcc
     1261 gcattgatga cgcgaatctc ggtgctgccg cgaacgtaag cgatggttcc gacacgctga
     1321 agatcttttt gcgcgcgtgt agcaggaacc agcgacaaca gcgcgaccgt cagaagcgct
     1381 atgcccgcaa aaaccagaaa cgactctccg attaatcttt gcaacgtctt aaactccagg
     1441 tgatggtggc tttcctttcg ccacctttaa gtgttgggat tgttaccgta tcagcgccgc
     1501 tgatctcgtc cgggttgttt ggatcgatcc tacctttgcc gtaaagggtc ggtccttccg
     1561 cagtcagctt cgctccggtc tgaggctggc tttggcgaat acctttgtta aaaggattgc
     1621 ggcagctgcc tttgacttcg ctgacgatcg agtatgtccc cgaaccgtta acgacaattg
     1681 ttggcggcga cacactgtac tcacgcgttc gagggtttac agaaacactc cagccggatg
     1741 tcacgacggt gtctgaacct ttgagctgct gaatctgcgt cgaggttcca taacagacac
     1801 ccttaccagt tgacgtgcgc tctcgttttt gactggcggt ggccttaaca gtcgctagcg
     1861 gcgcgccgag attggttttt ttgcccgaaa tctgaatctc tgaatcatag tgctcctcgt
     1921 cattccacgt actcgtattt acgttgttct ccgcggagcc gacttctttc aaactcgccg
     1981 tgtaagtgat cgtgcccacc cactgaccct tacagggctc ccaatctttg actgggaagt
     2041 agaacggctt ggcgctgtac cagttggacc ggtacatcgt ttccattgcg acacctactc
     2101 cgccaccaat agcgtcgccg gtcaaaaatg aaattacatt tccaacaatg tcgccgagag
     2161 ttgaggccgc ggcctgcgcg tccttgatct tcatcggctt gatctgtacg cctaccgtga
     2221 cgccggccgc cttgtacact tcaaacaatt tccgttttga gagatcttca tcctgcggcg
     2281 cgccgatcac gtaaatttcg gagacgccat cgccattagc tgtttgcttg ttgggcatgg
     2341 aatgcgctcc gtctttcggc tttagataaa cgatatcgtc gcgatcgtct tcaccactga
     2401 ccagatcgat cagagtgccg agcgcgccgc ggctatcgcc gcccagaacg agtgaccacg
     2461 caactgttgt atccgctaat ggaccagact tgggaaactc gagatcgaga ccggcggcgc
     2521 tgaaaatggg acgcaaacag ttgagacgtt cccaggcact cttgtcgatc ttcagcgtcg
     2581 c
//
LOCUS       DASTEY010000300         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1473170, whole genome shotgun sequence.
ACCESSION   DASTEY010000300 DASTEY010000000
VERSION     DASTEY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473170"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1844
                     /locus_tag="VFO72_05265"
     CDS             219..1844
                     /locus_tag="VFO72_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478729.1"
                     /translation="MKKVHALLMMLILCTVSSMPLMVHAQSDTALRQRAEISNAIHFD
                     KTTKPLREMFDTGERPGPARGGKDFEPGRPQKVDNTNRPFDDPLADKSVGLPSALAEI
                     KASTNGVPVDPNARVAPPDTTGDVGPNHYVQWVNLRYSVYTLTRGANNEITGFTVVPG
                     FPKNGNVVWQGFGGRCQTDNDGDPIVQYDQLADRWILTQFAVSNRPYTQCVAVSTSPD
                     PTGTYFRYAFSYDRSFNDYPKMGVWPDAYYISYNMFRNGMSFTGNTVCAFERARMLLG
                     LSARQACVNTTGGHSLEPADLEGTIPPPAGSPNLLMSLTSTAVQFWRFSVNWVAGTGT
                     LTGPTNVAGVAAFSRACGGGACIVQPGTTTRLDSLADRLMYRLSYRNFGTHEALVINH
                     SVTSGTGVGIRWYELRNAAGQTFGSAAPVLHQQGTFAPTNDFRWMGSAAMDKTGGIAI
                     GYNISSSTIVPSIRYAYRGPADPLGTMGNETSALVGSGSQTGNLTRWGDYSTISVDPV
                     DGCTMVFTTQFQPANGSFNWTTFIHSFKLSTCN"
     gene            1922..2959
                     /locus_tag="VFO72_05270"
     CDS             1922..2959
                     /locus_tag="VFO72_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478730.1"
                     /translation="MSLWKNYMHRRERWYHQLDNNRLVRPFDWGLSYIVDHVNGDDPR
                     ELFRKHTARVMPASEEFYALPPINDYRLEDDQLTWTSAITTPSPENNIARGRFFPAKS
                     KNGRRSRNAVVVLPQWNAQPESHVEACRIFNFIGMSALRLTLPYHQQRKPAEIERADH
                     LVSTNIGRTIQSMRQAVLDTRAAVRWLKSEGYERVGILGTSVGSCIAFLAFAHETDLD
                     AGAFNHVSGYVADVVWHGLSTAHVRAGFADHLTLDELRTYWQPISPLPFMPRLARMGH
                     RPIRFIIARYDLTFPIDLSRDAIAEARRHKLPLDIVWLPCGHYTTAELPWKAIDAWKI
                     ATFFRKQFSRR"
BASE COUNT          660 a          878 c          872 g          689 t
ORIGIN      
        1 atccgccgtt tatttgagcg ccgccagtag gcagttccgg ctggcggttg agggcaccga
       61 atgctatact tcccgccccc cagagtgcgt tgttctctct ctgagggatc ctgcttacag
      121 tttgtgtgtt tgcgttgaag taagcgtagt tacttacctc ccaatactat cgcagttcgt
      181 aactcactta gccctgaaaa aatgtctgga ggatgtctgt gaaaaaagtt catgcgttac
      241 tgatgatgtt gattctttgt accgtttcct caatgcctct catggtccac gcccagagcg
      301 acaccgctct aagacaacgg gccgagataa gcaatgcgat tcacttcgat aaaaccacaa
      361 aaccactgcg cgaaatgttt gacaccggtg agcggccggg gcctgctcgc ggtggcaaag
      421 acttcgagcc aggcaggccg caaaaagtag acaacaccaa tcgcccgttc gacgatcctt
      481 tggctgacaa gagtgtgggc ttgccatctg ctcttgcgga gatcaaggca tcgactaacg
      541 gcgtgccggt ggatccaaac gctcgcgtgg cgccgcctga cacaactggc gacgttggac
      601 cgaaccacta cgtgcaatgg gttaacctgc gttactcggt ttacaccctg accagaggtg
      661 caaataacga gatcaccggg ttcaccgtgg taccgggatt tccgaagaac ggcaatgtcg
      721 tatggcaggg tttcggcggt cgctgtcaga cagacaacga cggtgatccg atcgtccagt
      781 acgatcagct cgcagaccgt tggatactga cgcagtttgc agtgtcaaac cgaccgtaca
      841 ctcagtgcgt agccgtatcg acgagtcctg atccgactgg aacgtacttt cgctatgcgt
      901 tctcgtatga cagaagcttc aacgactatc ccaagatggg tgtttggccg gatgcctatt
      961 acatcagcta caacatgttt cgaaacggca tgtcgttcac cggtaacacg gtgtgtgctt
     1021 ttgaacgcgc acggatgctg ctcggccttt cggctcgaca agcttgcgtt aacaccaccg
     1081 ggggccacag cctggaaccg gcggatctcg aaggcacgat accgccacca gccggctcac
     1141 cgaacctgtt gatgagtcta acgagtacag cagtccagtt ctggcgattt tcggtgaact
     1201 gggtggccgg caccggcacg ttgactggtc ccacgaacgt tgcgggcgtt gcggcattca
     1261 gcagagcttg cggtggcggc gcttgcatcg tccaaccggg aaccaccacg cgtctcgatt
     1321 cactggctga tcgtttgatg tatcgcttga gttatcgaaa cttcggtact catgaggcgc
     1381 tggtgattaa ccattcggta accagtggaa ctggtgtcgg tattcgctgg tatgagttgc
     1441 ggaatgctgc gggccagacc tttggctcgg ccgcgcccgt gcttcatcag cagggaacgt
     1501 ttgcgccgac caacgacttc cgctggatgg gcagcgccgc tatggacaag accggcggca
     1561 ttgccatcgg ttacaacatt tcatccagca cgatagtgcc gagcattcgt tatgcctatc
     1621 gtgggcccgc tgatcctttg ggaacgatgg gcaatgaaac gtcggcgctg gtgggaagcg
     1681 gatcacagac cggcaacctg acccgttggg gcgattacag cacgatcagt gtcgacccgg
     1741 tggatggctg cacgatggtc ttcacgacgc agttccaacc agctaacggc agtttcaact
     1801 ggacgacgtt tatccatagc ttcaagctgt cgacctgcaa ctagaacgca gattaacgcg
     1861 gatgaagacg gatttttccc gttttcatcc gcgtagatct gtgtggtagt atccgcaatc
     1921 cgtgtcgctt tggaagaatt acatgcaccg gcgcgagcgc tggtaccatc aactggacaa
     1981 caatcgcctc gtccgtccgt ttgattgggg tctgtcgtac atcgtcgacc acgtaaatgg
     2041 cgacgacccg cgcgaacttt tccgcaaaca cactgcgcgt gtaatgccgg caagcgaaga
     2101 gttctacgct ctgcctccaa tcaacgacta tcgtctcgag gacgatcaac ttacctggac
     2161 cagcgcgata acaacgcctt cgcccgagaa caacatcgcg cgtggcaggt tctttcccgc
     2221 aaagagcaaa aacggccggc gatcgcgcaa cgcggtggtc gtgctgccgc agtggaacgc
     2281 gcaaccggaa agtcacgtcg aagcctgccg gattttcaac ttcattggca tgtcggcgct
     2341 gcgcctgacg ctgccgtatc accagcaacg aaagcccgcc gagatcgagc gcgccgatca
     2401 tcttgtcagc acaaatatcg gtcgcacgat tcagtcgatg cgacaggcgg tgctggacac
     2461 gcgtgcggcg gtgcgttggt tgaagtcaga agggtacgag cgcgtgggca ttctcggcac
     2521 cagcgtcggc tcgtgtatcg cgtttcttgc gtttgcacac gagacggatc tcgacgcagg
     2581 tgctttcaat cacgtttcag gttatgtggc ggatgtggtg tggcacgggc tctcgacagc
     2641 gcacgtgcgc gccggatttg cggatcactt gaccttggat gagttgcgca cttactggca
     2701 gccgatcagc ccgctgccat ttatgccgcg actcgcgcgg atgggtcacc ggccgatacg
     2761 tttcatcatc gcgcgctacg atctaacgtt tccgatcgat ctttctcgcg acgcgatcgc
     2821 cgaagcgcgt cgccacaaac ttccgctcga catcgtttgg ctcccctgcg gccactacac
     2881 caccgccgag ctaccctgga aagcgatcga cgcctggaaa atcgcgacgt tcttcagaaa
     2941 acaatttagc cgcagatgaa cacggattgt ttatctgcgg ctaattctat ttggctaagg
     3001 gaaagatcgg ctcgatgtcg accggcacgg ccttcagctt atcgagggcc tgctgcatcg
     3061 gcggtctgat cacggcaaac ttgtccagga tcgcttttg
//
LOCUS       DASTEY010000301         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1044554, whole genome shotgun sequence.
ACCESSION   DASTEY010000301 DASTEY010000000
VERSION     DASTEY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044554"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFO72_05275"
     CDS             <1..351
                     /locus_tag="VFO72_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9478731.1"
                     /translation="EFHCDDAIEERSELVPSVQMQIYRIVQEAVNNIWRHAEATEVKM
                     FVTISKDGDFSLKIEDNGRPFKSQHTNSEGRGLANMRARAGLIDARISWEPREDGGTV
                     FTLRRAATANAPTA"
     gene            335..1234
                     /locus_tag="VFO72_05280"
     CDS             335..1234
                     /locus_tag="VFO72_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET9478732.1"
                     /translation="MRQLLSIAPEVADALRDHRPVVALESTVIAHGLPRPQNLQTARR
                     LQEVVREGGAVPATIAIIGGKLTVGLDDEEIRLLAENTDIRKISTRDIPIAVAQGWDG
                     ATTVASTSWIAHRAGIKVFATGGIGGVHRGALPDVSADLPELARTPIALVCSGAKIVL
                     DLPATREWLETHAVTVVGYKCDELPAFYSRGSGLPVDASANDPAAVAGIFRAQRDLGI
                     ESALLVVVPVPAKFEVPAEELQQVLTTALEDAQWKEITGPALTPFLLSQMAERSGGAT
                     LRANIALLENNARVAAQIAIELV"
     gene            complement(1437..2138)
                     /locus_tag="VFO72_05285"
     CDS             complement(1437..2138)
                     /locus_tag="VFO72_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9478733.1"
                     /translation="MIYVGTSGYSYKEWKGSFYPEQLSAKDMLAYYASRLQAVELNNT
                     FYRLPQPHMVESWKAQVPGDFRFSVKASQVITHFRRLKDAGAQTRSMLKTVSALEDRL
                     GAVIFRFPEDFKKDLGRLEKFLKELPAHPPVTFDFRHPTWFDDDVLELLRSQNRALCI
                     SDADVMCADHIDKTADWGYLRLRREQYSDADLKKWIKRVRAQDWKTTFVFFKHEDEGT
                     GPKLAARFIALSEKA"
BASE COUNT          500 a          608 c          620 g          464 t
ORIGIN      
        1 gagtttcact gtgacgatgc gatcgaggaa cgaagcgagc tcgtgccaag cgtgcagatg
       61 cagatttatc gtatcgtgca ggaagcagtg aacaacatct ggcgacacgc tgaagccact
      121 gaagtgaaga tgtttgtgac catttcgaag gacggcgact tctctttgaa gattgaagac
      181 aacggccgcc cgttcaaatc gcagcacacc aattcggaag gacgaggcct cgccaacatg
      241 cgcgctcgcg ccggcttgat cgatgcgcgc atctcgtggg agccgcgcga agatggcgga
      301 acagtgttta ccttgaggag ggctgccact gccaatgcgc caactgctta gcatcgctcc
      361 tgaagtcgct gacgcgcttc gcgaccaccg tcctgttgtc gcgctcgaat cgacggtgat
      421 tgcgcacggc ttgccacgtc cgcagaacct gcaaactgcg cgacgcttgc aggaagtggt
      481 tcgtgaaggc ggcgcggtgc cggccacaat cgcgatcatc gggggcaagt taactgtcgg
      541 gctcgacgac gaagagatcc gactgcttgc cgagaacact gatatcagga agatcagcac
      601 tcgcgacatt ccgattgctg tggcgcaagg ttgggatggc gcgaccaccg tcgcatccac
      661 gagctggatc gcgcatcgcg ctggaatcaa agtgtttgca acaggcggca tcggaggcgt
      721 tcatcgcggc gctctgcctg acgtctctgc ggaccttcca gaacttgctc gcactccgat
      781 cgctctcgtt tgctcaggcg caaaaatcgt cctcgacttg cccgcaacgc gcgaatggct
      841 cgaaacacac gcggtcacag ttgttggtta taaatgcgac gagctgccgg cgttttattc
      901 gcgcggcagt gggctgccag tagatgcgag cgcaaatgat ccagcggctg tggccggaat
      961 ctttcgcgcg caaagagact tgggtatcga atcggcgttg cttgtcgtcg tgcctgtgcc
     1021 ggcgaagttt gaagtgccag cggaggagtt acagcaggta ttaactacag cgttggaaga
     1081 tgcacaatgg aaagagatca ctggaccagc cttgacgccg tttttattga gtcaaatggc
     1141 cgagcgcagt ggcggcgcga ccttgcgagc aaacatcgcg ctgctcgaga acaacgcccg
     1201 cgtcgccgca cagattgcga tagaactggt ttgatgtgtt actcttaggc ttccccaaaa
     1261 gctatcaaaa atttcaggag gatccccatg gcggacgata agaacaagtg caagaatccg
     1321 agctgtacct gcccggcgca gccgggtggc aaatactgca gcgcatcatg tgaaggctca
     1381 ggcgacacga tcgagctcga ttgtgactgc gggcatgatg aatgcggcgg gaacttctag
     1441 gccttttcac tcagcgcaat aaagcgtgcg gcgagctttg gtccagtgcc ttcgtcttca
     1501 tgcttgaaga acacgaacgt agttttccag tcctgagctc gcacacgttt gatccacttc
     1561 ttaagatccg cgtctgaata ctgctcccgc cgcagacgca ggtaacccca atccgcagtc
     1621 ttatcgatgt gatcggcgca cataacatct gcgtccgaaa tacaaagtgc gcggttctgg
     1681 ctgcgcaata actccagcac atcgtcgtcg aaccaggtcg gatgtcgaaa gtcgaacgtg
     1741 acgggagggt gtgccggcag ttcttttaga aacttctcca gtcgcccgag gtccttctta
     1801 aagtcctcgg gaaatcgaaa aatcaccgca ccaagtcgat cttccaaagc tgaaacagtc
     1861 ttcagcatcg accgcgtttg cgcgccggcg tccttgagcc gccggaagtg tgtgattact
     1921 tgtgaggcct tcacggagaa acgaaagtcg ccaggcacct gcgccttcca gctctccacc
     1981 atgtgcggct gcggcagtcg atagaacgtg ttgttcagct ccaccgcctg caaacgcgac
     2041 gcgtaatatg cgagcatgtc tttagccgaa agctgttccg gataaaaact gcccttccat
     2101 tccttgtaac tgtagccgct ggtcccaacg taaatcattg ccgattgccg atttccaaat
     2161 taccaatttg tcagaaaaat cggcaatcgg ca
//
LOCUS       DASTEY010000302         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1473187, whole genome shotgun sequence.
ACCESSION   DASTEY010000302 DASTEY010000000
VERSION     DASTEY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473187"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..3408
                     /locus_tag="VFO72_05290"
     CDS             355..3408
                     /locus_tag="VFO72_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET9478734.1"
                     /translation="MGEGPRAQTGEPQPEATPSPSPSPSPSPSPTPITGLHQWGAVTL
                     FHGLPSDRVRAIAQTSDGAMWFGTEAGLAKFDGRRTQAINDPALPAGRVLALQVDQND
                     AMWVGTESGAARYSDGTFERIKETATQAVSAIVTGADGVTFMSTEPGSVFESRVGAGS
                     ALDTRALLSEPLRSADRDRPGPLAITSINRSNNRLFVGTLSRGVLEIENGAVKEPPAR
                     PAVFFVNALERDKDGRLWAGARARKEEPGAFTGTEIANLKRAEAATGPVMALKSIGDE
                     IWIGTDGRGVFRVSKAKTQRFTFDGTAGGLRSNRVYAIFVDREGVIWFGTDRGVCSFD
                     PHAPRVESIGDNSESNFVRALYQTSNGRTIAGTNRGLFVYDDEAAAWNLVPGLGQNII
                     YAVSEDRSQRLLVASVSGFYVSPRQSTPLEEQLFARLEAGAATADGVGSVRSIAQFNG
                     ATYYAIFGRGVERIDGGRASLTWSEGTPNTREVLSLLADGQERLLIGTVRDGVFVSDG
                     KTVREEPAFAILKGPAVRSMARTPDGSLWFGTSAGVYLCRATGGCTLALQHVDARFVL
                     AESGEANVIWCGTRGNGLVRILLDPVVGPVVSELDSEQGLPSQNAFAVLRQHKADGTD
                     VFLIGTNRGIARYEPGRFAPPLSVTRILSKRVHAPAELQAGLNLEYPQNSLLLDVTAI
                     SSRTFPEQFQYAFTLTDSSGQAVKQKLSRESQFTMEGLTPGRYTVTARAFTKDLTPSA
                     PLTFSFTVAQAPFPWTSTALAILLALALLALLWAILERRRIVATSAALVDANRELADA
                     RLNLANEAERERRRIARDLHDQTLADLRHLALLTDQIKTNGEPSGKQTSALRSEIESI
                     SQEVRRICEDLSPSVLQNVGFAAALEFALSHAVQDAPAEKRFEYEFHCDEAIEDRSEL
                     PPSVQMQIYRIVQEAVNNIWRHAGATEVKMFVNSSPEGDFSLRIEDNGRSFESQPANS
                     EGRGLANMRARAGLIDAQISWEPREGGGTIFTLTRKAARTLVPSH"
     gene            3408..4313
                     /locus_tag="VFO72_05295"
     CDS             3408..4313
                     /locus_tag="VFO72_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET9478735.1"
                     /translation="MRELLNIAPEVAAAIEASQPVVALESTVIAHGLPRPINLATAQR
                     LEGIAREAGAVPATIAVVAGKLCAGLDDAQLRALAGDSDVKKISTRDIPIAVAQRWTG
                     ATTVASTIWIAHRAGINVFATGGIGGVHRGSLPDISADLPELARTPIVVICSGAKIVL
                     NLPATREWLETHAVTVAGYGCDELPAFYSRSSGLPVDVRVEDAHDVAEIFHAQRALKM
                     SSALLVVVPVPEEFEVPAEQLMTVLGDALVEAERNTITGRELTPFLLSEMARRSQGAT
                     LQANIALLENNTRVAADIARALAER"
BASE COUNT         1021 a         1369 c         1442 g          995 t
ORIGIN      
        1 tccgctggag cgcactagtt aataaaaacg taaggctata aacatcttgc tcctccggag
       61 cacttctcag cagaagcatc aagtaaaaat cacttttcgg gcaaagccac ttcaactggc
      121 tttgcacgaa aggtacccaa atggcgtttg aggactactg tggaaaaaaa ttgtctcgcg
      181 caaaggcgaa aaggcgaaaa gaaaaccctt aaaaacgggg tagccgctct gcgcctttgc
      241 gcctttgcgc gagagttctt tcagaaagat atacttttcg ggcaaagcta cttcaacttt
      301 acacgctggt tgttagtttt ctgcgcgttt gtactttgcg gtctgtttct gaacgtgggt
      361 gaaggtccac gggcgcaaac cggcgagccg caaccagaag caacaccttc gccgtcaccg
      421 tccccatcgc cttcgccttc cccgacacca atcacgggtc tgcaccagtg gggcgccgtc
      481 accctgtttc acgggctgcc gtcggatcgc gtacgcgcga ttgcgcaaac aagcgatggc
      541 gctatgtggt ttggaactga agcaggtctc gccaaattcg acggccgcag aacacaggcc
      601 atcaacgatc cggcgttgcc ggctggacgt gtgctggcgc tgcaagtgga ccaaaacgac
      661 gcgatgtggg taggcacgga atccggcgcg gcgcgataca gtgacggcac attcgaaagg
      721 atcaaagaaa ctgctacgca ggcagtttct gcgatcgtca ccggagcaga cggtgtcaca
      781 ttcatgtcga ccgagccggg gagtgtgttt gagtcgcggg tgggcgcagg ttctgcactc
      841 gacactcgcg cgttgctaag cgagccgttg cgaagtgcgg atcgcgatcg acctggtcca
      901 ctcgccatta ccagcatcaa tcgatcaaac aaccgtctct tcgtcggcac tttaagccgc
      961 ggcgttttgg agatcgaaaa cggcgccgta aaagaacctc cggcgaggcc tgccgtgttt
     1021 ttcgttaatg cgttggaacg cgacaaagac ggccggctct gggcgggcgc gcgcgcaagg
     1081 aaagaagagc cgggagcatt taccggaacc gagatcgcga acctgaaacg cgccgaagca
     1141 gcgactggcc cagtcatggc gttgaagtca attggcgatg agatatggat cggcaccgac
     1201 ggccgcggtg tgtttcgtgt ttcgaaggca aaaactcaac gctttacctt cgacggcaca
     1261 gccggcggtc tccgttcgaa tcgcgtttac gcgatctttg tggatcgcga aggtgtgatc
     1321 tggttcggca ctgatcgcgg tgtctgtagc ttcgatccgc acgcgccgcg cgtcgagtca
     1381 attggcgaca acagcgagag caattttgtc cgcgcgcttt atcaaaccag caacggccga
     1441 accatcgcag gcaccaatcg cggcctgttt gtttacgacg acgaagccgc ggcctggaat
     1501 ctcgtgccgg gactgggtca aaacatcatt tatgcagtgt ctgaagaccg atcgcagcgt
     1561 ttgctggtcg cgtcagtgag cggtttctac gtctcgccaa gacagtcgac gccgttggag
     1621 gaacaactgt tcgcgcgact cgaggcgggt gcggcaactg cggacggtgt tggcagtgtt
     1681 cgctcgattg cgcagtttaa tggcgcgacc tattacgcca tcttcggacg tggcgttgaa
     1741 cggattgatg gcggccgggc gagtttaacc tggtctgaag ggacgcccaa cacgcgcgaa
     1801 gttctcagtt tgctcgccga cggacaggaa cgactgctga tcggcacggt aagagacggc
     1861 gtgtttgtgt cagacggcaa gacagtgcgc gaggagccgg ctttcgcgat cttgaaaggt
     1921 ccggcagtgc gctcgatggc ccgaacgccg gatggttcgc tgtggtttgg aacgtctgcc
     1981 ggcgtctacc tttgccgggc taccggtggt tgcactctgg cgctgcagca tgtcgacgcg
     2041 cgtttcgtgc tcgccgagtc gggagaggca aacgtgatct ggtgtggcac gcgcggcaac
     2101 gggttggtgc gcattctgtt agatccggtt gttggtccag tggtgtcgga actcgattcc
     2161 gaacagggct tgccttcgca gaatgctttc gccgtgttgc ggcaacacaa agctgacgga
     2221 actgatgttt tcctaatcgg aacaaaccga ggcatcgcgc gctacgaacc gggccgcttt
     2281 gcgccgcccc tttctgtgac acgcatcctg agcaaacgcg tacacgcgcc ggcggaactt
     2341 caggcggggt tgaatctgga gtatccgcag aacagtctgc tgctcgatgt gactgcaatc
     2401 agcagccgca catttcccga gcagtttcag tatgccttta cgctgactga cagcagcggc
     2461 caggcggtca aacaaaaact ctcgcgcgag tcgcagttca cgatggaagg gctgacgccg
     2521 ggcaggtaca cggtgactgc acgcgcgttc acgaaggatc tgaccccttc agctccgctt
     2581 actttttcct tcaccgtggc gcaggcgcca tttccgtgga cctcaaccgc gctcgcaatt
     2641 ctgctcgcac tcgcgctgct ggcactcttg tgggccatac ttgaacgtcg ccgcatcgtg
     2701 gccaccagtg ctgcgctcgt cgacgcaaac cgcgaactcg cggacgcgcg actcaatctc
     2761 gcaaacgaag ccgaacgcga acgccggcgc attgctcgcg acctgcacga tcaaactctc
     2821 gcggatctgc gtcatttggc gttgttgacc gaccagatca aaaccaacgg cgagccgtcg
     2881 ggcaaacaga cctccgcttt acgaagtgag attgagtcga tttcgcagga ggtgcgacgc
     2941 atttgcgaag acttaagtcc gtcggttttg caaaacgtcg ggtttgctgc ggcactcgag
     3001 tttgcattgt cacacgccgt gcaggacgct ccggctgaga agcgatttga gtacgagttt
     3061 cattgtgacg aagcgataga agatcgaagt gagttgccgc cgagcgtgca gatgcagatc
     3121 taccgcatcg tgcaggaggc agtgaacaac atctggcggc atgccggtgc gaccgaagtg
     3181 aaaatgttcg tcaacagctc gccggaaggc gatttttcgc tgcggatcga agacaacggc
     3241 cgttcgtttg agtcgcaacc ggcaaactcc gaaggtcgcg ggctggcgaa catgcgcgcg
     3301 cgggccggat tgatcgacgc gcagatttcg tgggagccac gcgaaggcgg cggcacgata
     3361 ttcacgctaa ccaggaaagc ggcacgcaca ctagtaccgt cacattaatg cgagagttgc
     3421 tgaacatcgc gccggaagtg gccgcggcga tcgaggctag ccaaccggtg gtagcgctcg
     3481 aatccacagt aatcgcgcac ggactgccac gtccgatcaa tctcgcaacg gcgcagcgtc
     3541 tggaagggat tgctcgtgaa gctggcgcgg taccggcgac cattgcagtt gttgccggta
     3601 agttatgcgc cggactcgac gacgcgcaac ttcgagcgct tgccggtgac agtgacgtca
     3661 agaagatcag cacgcgcgac attcctatcg cggtcgcaca acgctggacc ggagcgacga
     3721 ctgtagcttc gacgatctgg attgcgcatc gcgccggaat caatgtgttc gcaaccggcg
     3781 gcatcggcgg cgtgcatcgc ggttcgttgc cggacatttc cgccgacctg cctgagctcg
     3841 ctcgcacgcc aatagtcgtg atttgttcag gcgcaaagat cgtcctcaac ttgccggcca
     3901 cgcgcgaatg gctcgaaaca catgccgtaa cggttgccgg ttatggatgc gacgaactgc
     3961 cggcgttcta ttcgcgcagc agcggtctgc cggtcgatgt tcgcgtcgag gatgcacacg
     4021 acgttgcaga aatttttcac gcgcagcgag cgcttaaaat gtcatctgcg ttgcttgtag
     4081 ttgtgccggt gccggaggag tttgaagttc ccgcagaaca attgatgact gtgcttggcg
     4141 atgctttagt cgaggcggaa cgcaacacca tcactggtcg cgagttgacg ccgtttctct
     4201 tgagcgaaat ggcccggcgc agtcagggcg ccacgctgca ggcgaacatc gcgttgctgg
     4261 aaaacaacac gcgcgtggcc gctgacatcg cccgcgcact cgcagaacgt tgagaccatt
     4321 gatgcaattt gcgacccttg ttcctgggac cgcaggcgtc tcgcctgcac cgtggcggta
     4381 gtgatgcagt ttgaaaaccg gggcgccgtc ctcctgggac ggcaggcgtc tcgcctgcac
     4441 cgtgaagagt tgggcgctgg ccgtgagatg caggcgaaac gcgtgcggtc ccaccttcga
     4501 actgcatcaa tatccacgtt aaggaccagg cgagagttcg cctgcatcac agtttgttga
     4561 cttcgtaggc atataatttt ttgcagaccc tcaacaacca ttcattcgcg gctcacatct
     4621 cacggcacga aacaacctga gaccactccc caggctctcc aatcaaccta cctgaaagga
     4681 gtcagcctat gtccgaccag gactttgccc gtcagcttcg catcacatcg ccttgtaaaa
     4741 tggactggaa ctcaatggtc gggaacgagc gcgttcgttt ctgcgagcat tgccaccttt
     4801 ctgtccacaa catcaacttt gccagta
//
LOCUS       DASTEY010000303         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_929279, whole genome shotgun sequence.
ACCESSION   DASTEY010000303 DASTEY010000000
VERSION     DASTEY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929279"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFO72_05300"
     CDS             complement(<1..576)
                     /locus_tag="VFO72_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET9478736.1"
                     /translation="MATTTHIEEQPRVIIPRASRANEILAILLLAAGALLALCLVSAA
                     FYPNDPSWNAAGQGEIQNWAGSIGANVAATAFQFIGLASFLLPLLLFAAAWRRFRRNA
                     VHHAWARIAGLIVLVIAASSLLSISQVRPLFDASVQPGGLLGAVVSQGLASGLSNIGA
                     TVLLVAIAATGLLLATNFSFIRLYEIIVNAIG"
     gene            complement(885..1995)
                     /gene="prfB"
                     /locus_tag="VFO72_05305"
     CDS             complement(join(885..1922,1924..1995))
                     /gene="prfB"
                     /locus_tag="VFO72_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET9478737.1"
                     /translation="MSDLLTIQELRGSYDNLRARVDQLGGFFDVPARREELSRIEAQA
                     SAPGFWNDQEAAQKLLQNRSSLEKKIERQEKFDSQIADGEVLFEFAEEDESSLKELQT
                     LLARLEHDLSQAETEMLLAGDNDRRNAICTIHPGAGGTESQDWAEMLLRMYLKWAEQR
                     GFKAEVLEYQPGEEAGLKSVTFRVEGEYAYGLLAAEAGVHRLVRISPFDQAARRHTSF
                     ASLFVYPEIDEDIEVEINDKDLRVDTYRATGAGGQHINTTDSAVRITHIPTGIVVSCQ
                     NQRSQHQNRAVAMQVLRSRLYELELEKRRAETAELEANKADISFGSQIRSYVLAPYQL
                     VKDTRTKLERGNVDAVLGGDIDDFIKAYLLARKAA"
BASE COUNT          445 a          580 c          572 g          448 t
ORIGIN      
        1 accgattgcg ttgacgatga tttcgtagag acgaataaac gagaagttgg tggcgagcag
       61 tagcccggta gcggcgattg ccaccagcag gacagtcgca ccgatgttgc tcaagccgct
      121 ggccagtcct tgtgaaacaa ccgcgccgag cagcccgccg ggctgtacgc tggcgtcgaa
      181 gagcggcctc acttgggaga ttgagagcag ggatgaagcg gcgattacca gcacgatcaa
      241 tccggcgatc cgcgcccacg catgatgaac cgcgttgcga cggaagcgcc gccatgcagc
      301 agcgaacagc agcagcggga gaagaaacga agcgagtccg atgaactgga aggctgtagc
      361 ggcgacgttg gcgccgatcg agccagccca gttttgaatc tcgccttgtc cggctgcgtt
      421 ccacgaagga tcgttggggt agaaggcagc cgagactaaa cagagcgcga gcaacgctcc
      481 ggcagcgaga agtaaaatcg cgagaatctc gttagccctg ctcgcgcgtg gaatgatcac
      541 gcgcggctgc tcttcgatgt gtgtggttgt agccattgca actccaaagg tccggcacaa
      601 gttcaaacgc cgaaggcgtt agcttccacg ccggagaaat ccggcacagg tccaaacgcc
      661 gaaggcgtta gctaattcca gcccagggtg gagcatgcga cgaccctggg aacaatcaga
      721 ttctgagtca caacgctgaa agcgttagcc aattcggttt ggtctcgcca acgccttcag
      781 cgttggttgt ctttcttatt gtgtgaccca gggttgtgcc aaccctgggc tagcattagc
      841 aaacgccttc ggcgttcgac ttttcaaacg ccgaccgcct atctctatgc agccttgcga
      901 gcgagcaagt aggctttgat gaagtcgtcg atgtctccac caagcactgc gtccacgtta
      961 cctcgttcca gttttgtgcg agtgtctttt actaactgat acggcgcgag cacatagctt
     1021 ctgatttgag atccaaacga gatgtcggct ttgtttgctt cgagttcggc ggtttcggcg
     1081 cgtcgctttt ccaactcgag ttcgtaaaga cgcgaacgca gcacctgcat cgcgacggcg
     1141 cggttctgat gttgcgaacg ctgattttga caggagacta caattccggt cgggatgtgc
     1201 gtgatacgca cggccgagtc ggtagtgttg atgtgctggc cgcccgcgcc cgtggcgcga
     1261 taagtatcga cgcgcaggtc tttgtcgttg atttcgactt cgatgtcttc gtcgatctcg
     1321 ggatagacaa acagggaagc aaaggaggtg tgacgccgcg cagcctgatc aaacggcgaa
     1381 atccgcacca accggtgaac gccggcttcg gcagcgagca gcccgtaggc atactcacct
     1441 tcaactctga acgtcacgct tttcagccca gcttcctcgc ctggttgata ctcgagaact
     1501 tcagctttga atcctcgttg ttcagcccac ttgagataca tgcgcaggag catctcggcc
     1561 caatcctgcg attcggtccc acccgcgccc gggtgaatgg tgcaaatagc attgcggcga
     1621 tcgttgtcgc cggcgagcaa catctccgtc tcggcctggc tcaagtcgtg ctcgagccgt
     1681 gcgagcagcg tttgcagctc ctttaaagag ctctcgtctt cttcggcaaa ctcaaagagc
     1741 acttcaccgt cggcgatctg cgaatcaaac ttttcctgac gctctatttt cttttccagc
     1801 gaactacggt tctgcaataa cttctgggcg gcttcctgat cgttccagaa accaggcgct
     1861 gaggcctgcg cttcgatccg gctcaactct tcgcggcgtg ccggaacatc aaagaaacct
     1921 cccgagctgg tctacacgtg cacggaggtt gtcgtacgaa cctctcaatt cctggattgt
     1981 taaaagatca ctcatctcgt ctgtctaaaa ctccttctac tactgaacag gatcgcaaag
     2041 aacac
//
LOCUS       DASTEY010000304         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1061407, whole genome shotgun sequence.
ACCESSION   DASTEY010000304 DASTEY010000000
VERSION     DASTEY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061407"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFO72_05310"
     CDS             <1..573
                     /locus_tag="VFO72_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9478738.1"
                     /translation="TFQHVLEGYKVAKEIAAHGAGASIFADSWSYKIEAYDAIPYNAA
                     IMTRAGVVVSMNSDSDERARRLNIEAAKAMHWGDLTEEQALRLITLNPAIQLGVQDRV
                     GSIEVGKDADLAIWNGHPFSVYSRVDTTFVDGVVFFDRQQDLTRRSELAKERATLEQA
                     EPNRPPAQGQSPQRPRRPRTQDDTIEEEQP"
     gene            570..1928
                     /locus_tag="VFO72_05315"
     CDS             570..1928
                     /locus_tag="VFO72_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9478739.1"
                     /translation="MNLPKRFANLALVMSICMICGPTLLGQQIGNPTTQQGVLPPRGV
                     FAIRNARIVTVSGPDIENGTLVIRDGKIEAVGAAVNVPPGAQTIDGRGLSVYPGMIDA
                     GTNMGLVEVPQGANGTVDLSEVGDFNPNAKAITAVNPHSAHIAVTRVEGVTNTVTAPT
                     GGLISGQATLINLLGTAPKEMAVVPQAALVINYPRIGGGGGGGFGPPQAPANLSETLE
                     ANARQLDQIRKMLRDGEAYGRAQDAYAKDKSLPRPDQNVVLEALVPYVRGQQPVIFRA
                     EREAEIRGAIRFAEEMKLKPIILGGNDAWKVASLLKEKNVPVILTGVFSLPVREDDAY
                     DTLFENPAKLHQAGVRFAISTGDSGPEVRNLAQYAGMAAAYGLSKAEALKSVTLYPAQ
                     IMNVADRLGSIEVGKMANLVVTDGDLLEIRTRIRYLLIDGRSVVLSSRHTELNDAFKN
                     RR"
     gene            1934..>2129
                     /locus_tag="VFO72_05320"
     CDS             1934..>2129
                     /locus_tag="VFO72_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478740.1"
                     /translation="MLTRHHFAFTLAAVLLFAAAAQAQTKLLRFPDIRGDRVVFTYAG
                     DLWTAPSTGGNAIRLTSHAGV"
BASE COUNT          445 a          607 c          655 g          422 t
ORIGIN      
        1 acgttccagc acgtgctcga aggctacaag gtggcgaaag agatcgccgc gcatggcgct
       61 ggcgcttcta tctttgccga ttcgtggagc tataagattg aagcttacga cgccattcct
      121 tacaacgcgg ccattatgac gcgcgcgggc gtggttgttt cgatgaactc cgattctgac
      181 gaacgcgcgc gccgcttgaa tatcgaggcg gcaaaagcga tgcactgggg cgacttgact
      241 gaagaacagg cgctccggct gatcacgctc aaccccgcga tacaactcgg tgtccaggac
      301 cgcgtcggct cgattgaagt cggcaaagac gcggaccttg cgatctggaa cggccatccg
      361 ttcagcgttt attcgcgcgt agatacgacc tttgtcgacg gcgtggtttt ctttgaccgc
      421 cagcaggacc tcacgcgccg atccgaactc gcaaaagagc gtgcaacgtt agagcaagct
      481 gagccaaaca ggccgccggc acagggacag tcgccgcaga gaccacgccg tccgcgaaca
      541 caagacgaca cgatcgagga ggagcagccg tgaaccttcc aaagagattt gcgaacctcg
      601 cgttggtcat gtcgatctgt atgatctgtg gcccaacact cctgggccaa cagatcggca
      661 acccaacaac gcaacaaggc gtgctgccgc cgcgcggtgt gtttgcgatt cgtaacgcgc
      721 gtatcgtgac cgtaagtggt cccgacatcg agaacggtac cctcgtgatc cgcgacggca
      781 agatcgaagc agttggcgcg gcggtaaacg ttccgccagg cgcacagacg atcgatggcc
      841 gcggtttgtc ggtttatccg ggcatgatcg acgccggcac gaacatggga ctcgtcgaag
      901 taccgcaggg cgcgaacggc accgtcgatc tttccgaagt cggcgacttc aatcccaacg
      961 cgaaagcgat caccgctgta aatccacaca gcgcgcacat cgccgtcacg cgagttgaag
     1021 gagttacgaa caccgtgacc gcgccgaccg gtggtttgat ttccggccag gccacgttga
     1081 tcaacttgct cggcaccgcg ccgaaagaga tggcggtcgt gccacaggcg gcgctcgtca
     1141 tcaactaccc gcggatcggt ggcggtggtg gcggaggttt tggtccaccg caggcgcctg
     1201 cgaacctgtc ggaaacgttg gaagcgaacg cgcggcaact cgatcagatc cgcaagatgt
     1261 tgcgggatgg cgaagcttac ggacgcgcgc aggacgctta tgctaaggac aaatcgttgc
     1321 cgcgtccgga tcagaacgtg gtgctcgaag cgcttgtgcc ttatgtgcgc ggccagcagc
     1381 cggtgatctt tcgtgctgaa cgcgaggcgg aaatccgcgg cgcgatccgg ttcgccgaag
     1441 agatgaaact caagccgatc attctcggcg gcaacgatgc gtggaaggtc gcgagtttat
     1501 taaaagagaa gaacgttccg gtgattttga cgggcgtgtt ctcgcttccg gttcgcgagg
     1561 acgatgccta cgacacgttg tttgagaatc ccgcgaaact ccatcaggcg ggcgttcgtt
     1621 tcgcgatttc aaccggcgac agcggaccgg aagtgcgaaa tctggcgcaa tacgcgggca
     1681 tggccgccgc ctacggcctt tcgaaagccg aggcgttgaa gtcggtgacg ctttatcccg
     1741 cgcagatcat gaacgtcgct gaccggctgg gctcgatcga ggtcggaaaa atggcgaatc
     1801 tcgtcgttac ggacggcgat ctgctcgaaa ttcgtacccg catccgatat ttgttaatag
     1861 atggccgttc ggtggtgtta agtagtcgtc acactgaatt gaacgatgca ttcaagaatc
     1921 ggaggtagtt cttttgttaa ccaggcatca ctttgctttc acgcttgcgg cggtgctcct
     1981 ttttgccgcc gccgcccagg cgcagacgaa gctgctgcgt ttccccgaca ttcgcggtga
     2041 tcgcgtggtg tttacgtacg ccggcgatct ttggaccgcg ccttcgacgg gcggcaatgc
     2101 gatccgtttg acgtcgcatg ccggcgtcg
//
LOCUS       DASTEY010000305         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1604929, whole genome shotgun sequence.
ACCESSION   DASTEY010000305 DASTEY010000000
VERSION     DASTEY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604929"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFO72_05325"
     CDS             <1..668
                     /locus_tag="VFO72_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9478741.1"
                     /translation="LPLEVAEFFAAAGLPILQAYGLTENVCVAFNRPDRFKFGTVGPP
                     MPGCEVRIAADKEILVRSEMMFSGYYKAPEETAKVFSDGWLLTGDLGEVDEDGFLKII
                     GRKKELIVTSTGKKVAPALLENMLKESHVISQAMVYGEGRSYLVALITLNAPAERETV
                     QAIIDGVNRRVSSTESIKRFAILERDFEIATDEITPTGKLKRDVITNRYRNEIERLYL
                     ATD"
     gene            674..1531
                     /locus_tag="VFO72_05330"
     CDS             674..1531
                     /locus_tag="VFO72_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9478742.1"
                     /translation="MKTRITLLIVVTFCLATLANAQTPKETTVFGQKIHYVEAGSGPN
                     VILLHGLGGSTQAWQFNIGPLAEKFHVYVPDQIGFGKSDKPLVMYRIRTYVDFLDQFC
                     KQLKIERATLVGNSMGGWIAAAFTAAFPDRVDKLVLVDAAGYAPPKELDMRTLFALNP
                     TTREGMKVLTAKVFYNKAFQTDAVIDMAIAARLAAGDGFTIKSITESIIRGEDFLDDT
                     VKTIKRPTLIVWGREDGLVPLAEGERFHKDIAGSKLVVFDQCGHVPNFEKPGEFNAAV
                     IKFLTTDAQ"
     gene            1533..1940
                     /locus_tag="VFO72_05335"
     CDS             1533..1940
                     /locus_tag="VFO72_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET9478743.1"
                     /translation="MNFTVGDSAEIKKTIEQADIDAFAEVTGDHNPVHVDEEFAKTTR
                     FGRRIAHGMLTASLISSVLANKLPGEGSVYLNQTLQFVAPVFPGDEITARVTVKEIRE
                     DKPILKLETICMNQRDEIVIRGEATVLAADKRR"
     gene            1937..>2451
                     /locus_tag="VFO72_05340"
     CDS             1937..>2451
                     /locus_tag="VFO72_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9478744.1"
                     /translation="MNTDQNSVRSYSWYALGLLTLVYVFNYLDRTIIYILLPLIKREM
                     AFTDLQLALLGSMSFVIFFTVLGIPFGRLADRAVRKNMIAAGLAVWSLFSGLTGFATG
                     FWSLLICRMMVGVGEATLGPAALSLLSDYFPPRVRATVQAIYSSGIAIGAGLAFFLGG
                     WLGQYYGWRVA"
BASE COUNT          559 a          636 c          680 g          576 t
ORIGIN      
        1 cgttgccgct ggaagttgcg gagttctttg ctgccgcagg cctgccaatc ctgcaggcat
       61 acggtctcac ggaaaacgtt tgcgtcgcat tcaatcgtcc ggacagattc aagtttggta
      121 cggttggacc accgatgccg ggctgtgaag ttcgcatcgc ggcggacaaa gagatcctgg
      181 tgcgcagtga aatgatgttc agcggttact acaaagcgcc ggaagagacg gcaaaagtat
      241 tcagcgacgg gtggctgtta accggcgatc tcggtgaagt tgacgaggat ggttttctaa
      301 agattatcgg tcgcaagaaa gagttgattg tcacgtcgac cggaaagaaa gtcgcccccg
      361 cattgctcga aaacatgctc aaagaaagtc atgtgatcag ccaggccatg gtttatggcg
      421 aaggacgaag ttacctcgtg gcactgatca cgttgaatgc acccgcggag cgtgaaacgg
      481 ttcaagcgat aattgacggc gtgaatcgac gcgtttcgtc gactgagtcg atcaagcggt
      541 ttgcgatttt ggagcgtgat ttcgagatag cgacggatga gattacgccg accgggaaac
      601 tcaagcgcga tgttatcact aatcgttatc gtaatgagat cgagagactt tatttagcca
      661 cggattaaca cagatgaaaa cacggatcac actacttata gtcgtcacgt tttgtctggc
      721 gacactagcc aatgcgcaaa ctcctaaaga aaccaccgtc tttggacaga agattcacta
      781 cgtcgaggcg ggttccgggc cgaacgtgat tttgttgcac gggcttggcg gttcaacgca
      841 ggcgtggcaa ttcaacatcg gtccactcgc ggaaaagttt cacgtgtacg tgcccgatca
      901 gatcggcttt ggcaagtctg acaagccgct ggtcatgtat cgcattcgta cttacgtcga
      961 ctttctggat cagttttgca agcaactgaa gatcgagcgc gccacgctgg tcggcaactc
     1021 gatgggcgga tggatcgcgg cggctttcac cgctgctttt ccggatcggg tcgataagct
     1081 cgtgttggta gacgctgcgg gttatgcgcc gccgaaagaa cttgatatgc ggacgctttt
     1141 tgccctgaat ccgaccaccc gagaaggaat gaaagtcctc acagccaaag ttttctacaa
     1201 caaggcattt caaaccgatg ccgtgatcga tatggcgatt gctgctcgcc ttgccgccgg
     1261 cgatggcttc acaatcaaaa gcatcaccga gtcgatcatc cgcggcgaag actttctcga
     1321 cgacactgtg aagacgatta aacgtcccac gctgatcgtc tggggacgcg aggatggtct
     1381 cgtgcctctg gccgagggag agcgctttca caaagatatc gccggctcaa agctcgttgt
     1441 cttcgatcag tgtggccacg ttccgaactt cgagaaaccc ggggagttca atgcggcggt
     1501 aatcaagttt ctcactacag acgctcaata agatgaactt tactgttggc gatagcgccg
     1561 aaatcaaaaa gacaatcgag caagctgata tcgatgcatt tgccgaagtg actggcgatc
     1621 acaatcccgt tcacgttgac gaagagtttg cgaagacgac tcgctttggg cgacgcatcg
     1681 cccatggcat gttgacggcg tcgctgatct cgtcggtgct ggcaaacaaa ctgcccggtg
     1741 agggaagcgt ctatctcaac caaacgctgc aattcgtcgc gcctgttttc cccggtgatg
     1801 agatcacggc gcgcgtcact gtgaaagaaa ttcgcgaaga caaacccatc ctcaaactcg
     1861 agacgatctg tatgaatcaa cgcgacgaaa tcgtcattcg tggtgaagca actgttttag
     1921 ccgcggataa acgcagatga atacagatca gaactcggtt cgcagctatt cctggtatgc
     1981 gctcgggttg ttgacgctgg tttacgtctt caactatctc gaccgtacca tcatttacat
     2041 cctgctgccg ctgatcaaac gcgagatggc gttcaccgat ttgcagctcg cactgctcgg
     2101 cagcatgtcg tttgtcatct tcttcaccgt cctcggaatt ccttttggac gtctcgccga
     2161 tcgcgccgtg cggaaaaaca tgatcgccgc cggtctcgcg gtttggagtc tcttttcagg
     2221 tctcaccgga tttgcaaccg gtttctggtc gttactgatc tgccgcatga tggtcggcgt
     2281 cggagaagcg acccttggac cagcagcatt atcgttgttg agcgactact tcccgccgcg
     2341 cgtgcgagcg acggtacagg ccatctactc atccgggatc gcgatcggcg ccggcctggc
     2401 tttcttcctc ggcggctggt tgggccagta ctacggctgg cgtgtcgctt t
//
LOCUS       DASTEY010000306         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1473255, whole genome shotgun sequence.
ACCESSION   DASTEY010000306 DASTEY010000000
VERSION     DASTEY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473255"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1266
                     /gene="hemE"
                     /locus_tag="VFO72_05345"
     CDS             226..1266
                     /gene="hemE"
                     /locus_tag="VFO72_05345"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET9478745.1"
                     /translation="MTQLLENSPFMRACRREPVPYTPIWLMRQAGRYMREYRDVRAKT
                     SFLELCKTPSLAAEVTVTAVERLGVDAAIIFADILLILEPMGIDLEFAAGEGPVIRNP
                     VRNASDVDRLLELEDVGALEFVNEAIRQTRRALKPDIPLIGFCGAPFTLASYVIEGGG
                     SKNYVHTKRLMYDDPGAWHAMLGLISRGLAKYLNGQIAAGAQAVQLFDSWVGCLSPDD
                     YREFVLQHTRDVIRNVTPGVPVIHFGTGTATLLELMREAGGDVIGLDWRIRLNEGWER
                     VGHDVAVMGNLDPVVLFAKQDVIREQAKRIVEQAAGRAGHIFNLGHGILPETPVENVI
                     ALVEMVHELSRG"
     gene            1273..2238
                     /locus_tag="VFO72_05350"
     CDS             1273..2238
                     /locus_tag="VFO72_05350"
                     /inference="COORDINATES: protein motif:HMM:NF000689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET9478746.1"
                     /translation="MKTDFSSGYDALLLVSFGGPEGMDDVIPFLENVLRGRNVPRERM
                     LQVAHHYELFAGVSPINQQNRDLIAALKNELETNGPRLPIYWGNRNWHPMLPDTVERM
                     AADGVENALAFVTSAYSSYSSCRQYLQNISDAQAKVGPRAPRIEKLRVFYNHPLFIEA
                     NVDHVRAVWNHPAHLVFTAHSIPESMAANCDYAAQLMETGGLIARALNIESWELVYQS
                     RSGSPSQPWLGPDVSDHLRALADQGVKDVVVAPIGFVSDHMEVVYDLDYEAQKVAREL
                     GINMVRARTAGTHPAFVKMIRELMRERINNEVPANICAPDCCRLT"
     gene            2246..>3603
                     /gene="hemG"
                     /locus_tag="VFO72_05355"
     CDS             2246..>3603
                     /gene="hemG"
                     /locus_tag="VFO72_05355"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET9478747.1"
                     /translation="MSDRIVIIGGGISGLAAAHRVLELKPAAQVTILEAANRLGGTIQ
                     TESRDGVLIERGPDSFISEKPQAVALAKRLGIESRLIETNEKYRRSFIVRNGRLRPVP
                     EGFQLLAPSRIWPFITSDIFSLTGKARMAADLILPRKRVNGATDESLASFVRRRLGVE
                     ALDRMAQPMVGGIYTADPESLSLRATLPRFLEMERKHRSLILAMLRQGRAQKSGTSGA
                     RYSLFLSFDRGVQVLVDAVTRINADFRVKTRAQTLTFDQGWTITTDTGEVFKADAVCL
                     AVPAYIAASLLTGELADKLRRIEYASTATINFGYRRAAIAHPLNGFGFVVPFIEKRSL
                     IACTFSSVKFSGRAPEGHVLLRAFAGGALQPEIFALDESELAARVEADLRELLGINEA
                     PLFVEVAKWERSMPQYEVGHLDRVASIEARLRAWPGLALAGGAYRGVGIPDCIRSGET
                     AA"
BASE COUNT          726 a          904 c         1157 g          816 t
ORIGIN      
        1 ggacttgtgg caagattctg ccacggagac agtttgcggt attcgcgggg tattgtagtc
       61 ccgaaccccg ttttgcacca atattccagc ggtgtcttaa gttagtgtct caagggggcg
      121 atccgttgcg ttgaacgtcc cggcgagagt taaagttcgg ccacagtttt gcgattacga
      181 accagatctg cgctgatagc cgaaatatcc gtataatctc ctctcttgac tcaattgttg
      241 gaaaacagtc cgtttatgcg ggcgtgccgc cgggagccgg taccgtacac accgatctgg
      301 ctgatgcggc aggcgggacg ctacatgcgc gagtatcgcg acgtccgcgc caaaacaagc
      361 tttctcgagt tgtgcaaaac gccgtcgctg gccgctgaag tgaccgtcac cgccgtcgag
      421 cgacttggcg tggatgcggc catcatcttt gccgacatcc tgctgatact cgaaccgatg
      481 ggtatcgatc tcgagtttgc ggccggcgaa ggacccgtca ttcgcaatcc ggttcgcaat
      541 gcttcagatg ttgaccgact ccttgagctt gaagacgtgg gcgcgctgga gtttgttaac
      601 gaagcgattc gacaaacgcg ccgggcgttg aagcctgaca taccgctgat tgggttctgt
      661 ggcgcgccgt ttacactggc gtcttacgtc attgaaggcg gcggctctaa gaattatgtg
      721 cacacaaagc ggctgatgta cgacgaccct ggcgcctggc acgcgatgtt gggactgatc
      781 tcacgaggtc tcgcgaagta tctgaatgga cagatcgcgg ctggagcgca ggcggtgcaa
      841 ttgtttgact cgtgggtggg ttgtttgagt ccggatgatt atcgtgagtt tgtgttgcag
      901 cacacgcgtg atgtgatccg caacgtgacg cccggtgtgc cggtgattca tttcgggacc
      961 ggaacagcga cgctgctgga gttgatgcgc gaagctggtg gcgatgtgat cgggctcgat
     1021 tggcgcatac gtctgaatga aggatgggaa cgtgtgggtc acgacgttgc cgtgatggga
     1081 aatctcgatc cggtggtgtt gtttgcgaag caggatgtga ttcgcgaaca ggcgaagcgt
     1141 atcgttgagc aagctgctgg gcgtgccggg cacattttta acctgggaca tgggattctg
     1201 ccggagacgc cggtggagaa tgtgattgcg ttagtcgaga tggtccatga attaagccgc
     1261 ggataaacac ggatgaagac agatttttca agtggttatg atgcgttgct gctggtgtcg
     1321 ttcggtgggc cggagggaat ggatgatgtg attccgttcc ttgagaatgt tttacgaggt
     1381 cggaatgtgc cgcgtgagcg catgcttcag gtcgcgcatc attacgagtt gtttgctggc
     1441 gtgagtccga tcaatcaaca gaatcgggac ttgattgcgg cgctgaaaaa cgaattggag
     1501 acaaacggtc cgcgacttcc catctactgg ggcaatcgca attggcatcc gatgctgccc
     1561 gatactgtcg agcgcatggc tgcggacggc gtcgagaacg ccctcgcgtt tgtgacgtct
     1621 gcttatagtt cttattcgag ttgccggcag tatcttcaga acatcagcga cgcgcaggcg
     1681 aaagttggcc cacgtgcacc gcgcattgag aagctgcgcg tgttctacaa tcacccgctc
     1741 tttattgaag cgaacgtcga tcatgttcgt gccgtgtgga accatccggc gcatctggtg
     1801 ttcacggcgc acagcattcc ggaatcgatg gcggcgaatt gcgactacgc ggcgcagctc
     1861 atggaaacag ggggtttgat tgcgcgagcg ttgaatatcg agagctggga gctcgtttac
     1921 cagagccgca gtggttcgcc atcgcagccg tggcttggac cggatgtttc ggatcatttg
     1981 agagcgctcg ctgaccaggg tgtaaaagat gtcgtggtgg cgccgatcgg tttcgtctcg
     2041 gatcacatgg aggtggttta cgatctcgac tacgaggcgc agaaggttgc gcgagagctc
     2101 ggcattaaca tggtgcgggc gcggactgcc gggacgcatc ctgcgtttgt gaagatgatc
     2161 cgggaactga tgcgggagcg aatcaacaac gaggtaccag caaacatctg cgcgcccgat
     2221 tgttgtcgtc tcacttagag gttacatgag cgacagaatt gtcatcatcg gcggcggcat
     2281 ttccggtctc gcagcggcac atcgcgtcct tgagctcaaa ccagccgcac aagtaacgat
     2341 cctcgaagct gcaaacagac ttggcgggac gattcaaacc gaatctcgcg atggtgtcct
     2401 catcgaacgc ggacccgatt ccttcatttc cgaaaaacca caggctgtcg cgcttgcgaa
     2461 acgattgggc attgagtcgc ggctgatcga gacaaacgaa aagtatcgcc gcagtttcat
     2521 cgtccgcaac ggcagactgc gaccggtgcc tgaagggttt cagttgctgg cgccgtcgcg
     2581 catatggccg tttatcacga gtgatatttt tagtttgacg ggcaaagcgc ggatggccgc
     2641 cgatttaatt ctgccgcgca agagagtaaa cggcgcgaca gatgaaagcc tcgcgagttt
     2701 tgtgagacga cgattgggtg ttgaggcgct cgatcgcatg gcccaaccga tggtcggcgg
     2761 catctacacg gccgatccgg agtcgctcag cctgcgcgca acgttgccgc gatttttgga
     2821 gatggagaga aagcaccgga gtctgatact cgctatgctg cggcaaggcc gggcacagaa
     2881 gagtgggacc agcggagcgc gttatagttt gtttctttcg ttcgatcgtg gcgtgcaggt
     2941 gttggtcgac gcggtgacgc ggattaacgc ggattttcgc gtgaagacgc gagcgcaaac
     3001 attgaccttc gatcaagggt ggacgataac tacggataca ggcgaggtct ttaaagcaga
     3061 tgcggtctgt ttggctgtac cggcttatat cgcagcgtca ctcttaactg gagagctcgc
     3121 agacaaactg cgccggatcg aatacgcctc caccgccacc atcaacttcg gttatcgtcg
     3181 cgcagccata gcgcatccgt tgaatggatt cggcttcgtc gtgcccttca tcgagaagcg
     3241 atccctgatt gcgtgcacgt tcagcagcgt gaagttctcc gggcgtgcac cagaggggca
     3301 tgtactgctg cgagcgtttg cgggcggtgc gttgcagccc gagatctttg cgttggatga
     3361 atcggagttg gcggctcgcg ttgaagcgga tttgcgtgag ttgttgggca ttaatgaggc
     3421 gccgctgttc gtcgaagtcg caaaatggga gcgctcgatg ccgcagtacg aggtgggcca
     3481 cctcgaccgc gtggcgtcga tcgaggcgcg gctccgggcc tggccagggc tggccctggc
     3541 gggcggggcc tatcgcgggg tcgggatccc cgactgcatc cgctcgggcg agaccgcggc
     3601 cga
//
LOCUS       DASTEY010000307         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1127088, whole genome shotgun sequence.
ACCESSION   DASTEY010000307 DASTEY010000000
VERSION     DASTEY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127088"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..592
                     /locus_tag="VFO72_05360"
     CDS             59..592
                     /locus_tag="VFO72_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET9478748.1"
                     /translation="MAAFCAMRIELENLEGGKSDFAHVYQPEELNPVDERVQLTEPAA
                     VTGKVRLSGNEVFVNGHVETRAQAECDRCLKTVELPVSADFALEYITGAEYEANGGAA
                     ELTEAEMSVSVFDGEAIDVDEVVKEQILLAVPTRMLCREDCKGICPKCGTDLNAGECT
                     CVKDDIDPRWAALKNLT"
     gene            675..857
                     /gene="rpmF"
                     /locus_tag="VFO72_05365"
     CDS             675..857
                     /gene="rpmF"
                     /locus_tag="VFO72_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9478749.1"
                     /translation="MPNPKRRHSKARRDKRRAHDALRAPQTTLCPNCREPRLPHRVCP
                     KCGHYKGREVVTVKQS"
     gene            867..1952
                     /gene="plsX"
                     /locus_tag="VFO72_05370"
     CDS             867..1952
                     /gene="plsX"
                     /locus_tag="VFO72_05370"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET9478750.1"
                     /translation="MRSHSQHQVPLTGKVVLDAMGSDHAPNVEIDGALAATRDLGVGV
                     ILVGQAHKVEPELRRCGWRGDGDRGIELVDAAEVIAMDDPVATSVRRKKKSSLRIGSK
                     LVMEGRADGFVSAGNTGAAMATAKMVIGTLPGVDRPALAAMIPTKAAKPTLLLDVGAN
                     SECKPHHLAQFAIMGDAYSRSVLGTAKPSVGLMSIGEEEAKGNDLTKEAFPLLRQLED
                     INFVGNVEGRDVFSGMIDVIVTDGFTGNVMLKLSEGLTEAMLSMLKKELMATAVTKAG
                     AMLAKPAFRSIKKRLDYTEYGGAPLLGVSRIVVIGHGRSNARAIRNAIRSVKDFSEHR
                     TAERIQEGLNNPQITQIYSNSSLRVSV"
BASE COUNT          491 a          509 c          629 g          438 t
ORIGIN      
        1 ctccaaggag gcgcctgata tgcaggccgt gttgatccgg tgaaatctgt agcctataat
       61 ggccgctttt tgtgcgatgc ggattgagtt ggagaacctg gagggcggca agagcgattt
      121 cgcccacgtt tatcagcctg aggaactgaa tcccgtcgac gagcgcgtac aactgactga
      181 gcccgcagcg gtaacgggta aagttcggct ttcgggaaac gaggtctttg tcaacggtca
      241 cgtcgagacg cgcgcacaag cggaatgcga tcgctgtctg aaaaccgtcg agctgcctgt
      301 cagcgcggat tttgcgctgg agtacatcac cggcgccgag tacgaagcta acggcggtgc
      361 ggcggagtta acagaagccg agatgtcggt ttccgttttt gacggtgaag cgatcgacgt
      421 cgacgaggtc gtgaaagaac agattttgct ggccgtaccc actcgcatgc tctgtcgcga
      481 ggactgcaaa ggcatttgcc cgaaatgcgg cacggatttg aatgcgggcg aatgcacgtg
      541 cgttaaagat gatatcgatc cgcgttgggc ggctctgaag aacttaactt aagccacaaa
      601 gaggcacaaa aagcacaaat ggttttcgtt tgcgcttttt gtgcttcgtt gtgattatta
      661 aatacggagt aattatgcca aatcctaagc gacgacattc aaaagctcgt cgagacaaac
      721 gtcgcgcgca cgatgccttg cgcgcccccc aaactaccct ttgcccgaat tgtcgtgagc
      781 cccgcttgcc acaccgcgtt tgccccaaat gcggccatta caaaggtcgc gaagttgtga
      841 cggtaaaaca aagctaaacc aaatttgtgc gaagtcactc gcagcatcag gtcccgttga
      901 cgggcaaggt cgttctcgac gccatgggaa gtgaccacgc gccgaacgtg gaaatcgacg
      961 gcgcccttgc tgcgacgcgt gatctcggcg tgggagttat tctcgtgggc caggcccaca
     1021 aggttgagcc tgaattacga cggtgcggtt ggcgcggcga tggcgaccgc gggattgagt
     1081 tagtcgatgc ggctgaagtc atcgcgatgg atgatccggt cgcaacttcc gtgagacgga
     1141 agaagaagag ctctttgaga atcggctcga agctggtgat ggaaggacgt gctgacggtt
     1201 ttgtttccgc cggcaacacc ggtgctgcga tggccactgc gaagatggtt atcggaacac
     1261 tgccgggcgt cgatagacca gcactcgccg cgatgattcc gacgaaagct gctaagccga
     1321 cgttgttgct ggacgtcggc gccaactcgg agtgcaaacc acatcacctg gcccagtttg
     1381 caatcatggg tgatgcttac tcgcgctcgg tgctcggcac agccaaacct tcggtaggat
     1441 tgatgagcat cggcgaggaa gaagcaaagg gaaacgactt gacgaaggaa gcgttcccat
     1501 tgctgcgaca gttggaggac atcaacttcg tcggcaatgt tgaaggacgc gacgtgttct
     1561 cgggcatgat cgacgtgatc gtcaccgacg gtttcaccgg caacgtcatg ctgaaactgt
     1621 cagaaggact gactgaggcg atgctttcga tgctgaaaaa ggagctgatg gccacggcgg
     1681 taacgaaggc cggggcaatg ctcgcgaagc ctgcgttccg gagtatcaag aagcggctcg
     1741 actacacgga atatggcggc gcgccgttgt tgggtgtgag tcgcatcgtc gtcatcggac
     1801 atggacgctc gaacgcgcgt gcgatacgca atgcaatcag gagtgtgaaa gacttttccg
     1861 aacatcggac ggctgaaaga attcaggaag gactgaacaa tccacagatt acacagatat
     1921 attcaaacag tagtctgcgt gtatctgtgt aatctgtgga tacttgtagt aatctgaggg
     1981 attgatggtg aatcaacgcg ctggagttct cggaactggg cattcctatc cggaaggcat
     2041 tctgacgaat gcggagctcg ctaacat
//
LOCUS       DASTEY010000308         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_978699, whole genome shotgun sequence.
ACCESSION   DASTEY010000308 DASTEY010000000
VERSION     DASTEY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978699"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFO72_05375"
     CDS             complement(<1..1236)
                     /locus_tag="VFO72_05375"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478751.1"
                     /translation="MDKRVSHASPRRDTENSPVDRGVVDLADARVKIRSRVRPTAESL
                     TQELDEVRSLLDQGLSTEAKARLALLIASARNYASILALARCALSVALEQQGHYRDSL
                     AAVSMYETPESRVKLDNQTASYLRVQIGLGYNYNGDHPKAIAMLKSTLRDYAESGGTN
                     LGHIYAALSRVYRSISEYPIARDYAQRALESFRQNGDWRGLAESYFGVGVADIHEGNY
                     ESGLENLEQALKLIGDRPAAYVLGRTYANMAGACWFLKRPQEGIDYLKKAITYYERTD
                     HKTNAADGYNNLGINLVLIGQWDRAQEALERALALATEVDERGAKVPMILDSLGELHM
                     LRGDLDQAKDYLSRAVALATENGNRWYAAQALRTLARSYGAIADPENALSKAREALSL
                     AESIGDRQAICESRLIAAEA"
     gene            1523..3019
                     /gene="ffh"
                     /locus_tag="VFO72_05380"
     CDS             1523..3019
                     /gene="ffh"
                     /locus_tag="VFO72_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9478752.1"
                     /translation="MFESLSDKLKRTLKNLRGEGVLTKEHVNAALREIRLALLEADVN
                     YKVAKDFIASVQQKAEGQQVWQELKPSEQVVKIVFDELVELLGGQSSRLVFTKQIPNT
                     VMVVGLQGSGKTTSTGKIARWLAANQDRKPLLLSVDVYRPAAREQLKVIAKATGQQIF
                     EDPQTNDPLTLVRGAIKHAQQTGFDTVLIDTAGRLHIDDELMEELVTIKNETHPVETL
                     FVADAMTGQDAVRSAEEFHRRVGITGVILTKMDGDARGGAALSIKQVTGQPVKFVGVG
                     ERYDALEPFYPDRVAQRILGMGDVLSLIEEVQAKVDQSEAEAQLKKLQQNEFTLDDFR
                     SQLRQVKKLGSFTKIMKLLPDQLLGGMGMPQLTEEQSKQMEQELKRTEAIIDSMTWEE
                     RNDHRILNANRRRRIARGSGTTVASVNQLIKQYVEMRQMMRQLSGSGLFGGSGLKGKM
                     MRKMTGMPDLAGFGGDGAEMPPMPSLPAAPTGGPSRKKLKKKRKKRRR"
     gene            3029..3298
                     /gene="rpsP"
                     /locus_tag="VFO72_05385"
     CDS             3029..3298
                     /gene="rpsP"
                     /locus_tag="VFO72_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9478753.1"
                     /translation="MLAIKLMRTGAKKSPSYRVVVKEKQSKRDGAYLENLGTYNPTRN
                     PAEIKLNLARVNYWLGKGAQPTDTVSRLIKASARAEKAAAAETQA"
     gene            3336..3569
                     /locus_tag="VFO72_05390"
     CDS             3336..3569
                     /locus_tag="VFO72_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9478754.1"
                     /translation="MRDAIEMIVKSLVDDKETVDVREIEQRNGATLLEVRVAPQDIGK
                     IIGKQGRTIRALRSLARIAGTKHNRRYLLEIVE"
     gene            3576..4103
                     /gene="rimM"
                     /locus_tag="VFO72_05395"
     CDS             3576..4103
                     /gene="rimM"
                     /locus_tag="VFO72_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9478755.1"
                     /translation="MDHAGSDDLIAIARAVRTRGLKGEIVAELLTDFPERFKDVDELV
                     LVSPSGERKLAQVEDYWFQKNRVVLKLADYDDVEAAKQLVGYDFAVHEADRVPLDEDE
                     FYDWELEGCTVKVGDKSIGRVQSVMKTGGAEILVITDGAGKEQLVPLAAEIVVNIDTA
                     ARVILIDPPEGLLDL"
     gene            4174..>4535
                     /locus_tag="VFO72_05400"
     CDS             4174..>4535
                     /locus_tag="VFO72_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478756.1"
                     /translation="MLRFDIITIFPEFFRETIDCGIVRRARNAGLVEIAAHDLRQWTT
                     DKHHVVDDRPFGGGDGMVLKPEPIFAGVEALTGAARREDLPSHTRVVLLSAQGEVFSQ
                     ALAQELAAEGAQSDSRIVL"
BASE COUNT         1046 a         1215 c         1322 g          952 t
ORIGIN      
        1 ggcttcagcc gcgatcaagc gcgactcgca gatcgcctgg cggtctccaa tgctctccgc
       61 gagtgacaac gcttctcttg ccttagagag cgcgttctcg ggatccgcta tggccccgta
      121 actacgcgcc agcgttctca atgcctgtgc ggcgtaccac ctgtttccat tttcggtcgc
      181 gagtgcaacc gcgcgactga ggtagtcttt cgcctggtcc agatcgccgc gaagcatatg
      241 cagttcgccc agcgaatcca gaatcattgg tactttcgcg ccgcgttcgt cgacttccgt
      301 cgccaaagcc aacgcacgct cgagcgcttc ctgagcgcgg tcccattgac cgatgagaac
      361 gagatttatt ccgagattgt tataaccgtc ggcggcattt gttttgtgat ccgtgcgttc
      421 gtaataagtg atggccttct tgaggtaatc gataccttcc tgcggacgtt tcaaaaacca
      481 gcacgcgccg gccatgttcg cgtacgttct gccgagcacg taagccgccg gacgatcgcc
      541 gatcagtttc aacgcctgtt ccaggttttc caaacctgac tcgtaattac cttcatgaat
      601 atcggctaca ccaacgccga agtacgattc cgctaacccg cgccagtcac cattctgacg
      661 aaaactttct aacgcgcgct gtgcataatc acgcgcgatc gggtattcgc tgatactgcg
      721 atacacgcgt gataacgcgg cgtaaatgtg tccgaggtta gtgccgccac tttcggcgta
      781 gtcgcgcagc gtcgacttca acatcgcgat cgctttcgga tgatccccgt tgtagttgta
      841 gccgaggccg atttgaacgc ggagataact ggcagtttgg ttgtcgagtt tgacccttga
      901 ttcgggcgtt tcgtacatcg aaactgcggc cagcgaatca cgatagtggc cctgttgttc
      961 gagggcgacc gaaagagcac aacgcgccag tgcaagaata gacgcgtaat tacgggcgct
     1021 tgcgatcagt aatgccaggc gcgcttttgc ttcggttgaa agcccctggt ccagcagcga
     1081 gcggacttcg tcgagttcct gcgtcaacga ttcggcagtc ggccgcacgc gcgaacgaat
     1141 cttcacacgc gcatctgcaa ggtcaaccac acctcggtcg acaggggagt tttcggtgtc
     1201 acgccttggg gacgcgtgac tgacacgttt gtccatttag tacctataaa agttcaatcg
     1261 agtggacttt tcattacact tcagagctgg gagggaaccg tttcacgtac tttaacttta
     1321 cggtttggcc gcgcaaacgg tttattcgtg gtgaatttaa cacatgaggc ggactgcgta
     1381 caggtttctc ccgtaaaaat tcggagacct cggtcgcccg cgaaatctcg tccgtgctgt
     1441 gatttacgcg ccttgacacc ccaaaacgcg gccgctatca tttccgtttc cgttcgcagc
     1501 gcgaacctac gcctagggtt atatgtttga gtcgctctcg gataaattaa agcgcacgct
     1561 gaagaatctg cggggcgaag gcgttcttac aaaggagcac gttaacgcgg cgctgcgtga
     1621 gatccgcctg gccttattgg aggccgacgt caactataaa gtcgccaaag actttattgc
     1681 ttcagttcag cagaaggcgg aagggcaaca ggtttggcag gaactcaaac cgtcggagca
     1741 agtcgtaaag atcgtcttcg acgaactggt tgagttgctc ggaggccaat cttcgcgcct
     1801 cgtatttact aaacagattc cgaacacggt gatggtggtc ggccttcagg gctcgggtaa
     1861 aaccacctcc accggcaaga ttgctcgctg gttggcggct aatcaggacc gaaagccgtt
     1921 gctgctttca gttgacgttt accgcccggc cgcacgcgaa cagttgaagg tcatcgctaa
     1981 agcgaccggc caacagatct ttgaagaccc gcaaaccaac gatccgctga ctctggtccg
     2041 tggcgcgatt aaacacgcac agcaaaccgg ttttgacacg gtgctcatcg acacggcggg
     2101 ccgcctgcac atcgacgacg agttgatgga agaactggtc acgatcaaaa acgagaccca
     2161 tccggtcgaa acattgtttg tagccgatgc gatgaccggg caggacgcgg ttcgctcggc
     2221 tgaagagttc caccggcgtg tcggcatcac aggcgtgatt ctgaccaaga tggacggcga
     2281 cgctcgtggc ggcgccgcgc tgtcgatcaa gcaagtcact ggtcagccgg taaagtttgt
     2341 cggtgtcggc gagcgttacg atgcgcttga gccgttttat cccgaccgag tagcgcaacg
     2401 cattctcggc atgggcgacg tactgtcgct gatcgaagag gttcaggcga aagtcgatca
     2461 gagcgaagcc gaagcgcagt taaagaagct tcagcagaac gaattcacgt tggacgactt
     2521 ccgctcgcag ttgcgccagg tcaaaaaact cggctctttt acaaaaatca tgaagctgct
     2581 gccggatcag cttctgggtg gcatgggaat gccgcagttg accgaagagc agtcgaagca
     2641 gatggagcag gagctgaaac gcaccgaagc catcatcgac tcgatgacct gggaggagcg
     2701 caacgatcat cgcattctga acgcaaaccg ccggcgccgg attgctcgcg gctcgggaac
     2761 gacagtagcc agcgtcaatc agctcatcaa gcagtacgtc gagatgcggc agatgatgcg
     2821 acagctttcc ggctcgggcc tgttcggtgg cagcggcctg aaaggcaaga tgatgcgaaa
     2881 gatgacgggc atgcctgatc tggcaggttt tggcggtgat ggagctgaga tgccgccaat
     2941 gccgtcgttg ccggctgcgc cgaccggcgg cccatcgaga aagaaattga agaagaaacg
     3001 aaagaagcga cgacgatagg agaacaaagt gttagcgatt aaattaatgc gcaccggggc
     3061 gaaaaagagc ccttcgtatc gagtggtggt caaagagaag cagtcgaaac gcgacggtgc
     3121 atatctcgaa aacctcggca cctacaaccc gactcgcaac cctgccgaga tcaaattgaa
     3181 cctcgcccgc gtcaactatt ggctgggaaa aggtgcgcaa ccaaccgaca ctgtgagccg
     3241 gttgatcaag gcgagtgcca gggccgaaaa ggccgcggct gccgaaacac aagcctaagc
     3301 agtctcttct tggacggaaa ccctgaacgg aagccatgcg agacgctatc gaaatgatcg
     3361 ttaagtcgct ggtcgacgat aaagagacgg tggatgttcg cgaaatcgag caacgcaatg
     3421 gcgccacgtt gcttgaagtg cgtgtggcgc cgcaggacat cggcaagatt atcggaaaac
     3481 agggccgtac catacgggcc cttagatctt tggcaagaat cgccggcacg aaacacaatc
     3541 gacgatacct tctggaaatc gtggagtgag gccagatgga ccacgccggc tccgacgact
     3601 tgattgcgat cgctcgagcg gtgcggaccc gcggattaaa gggcgagatc gtcgctgaac
     3661 tcctgaccga ctttccggag cgcttcaaag atgtcgacga actggttctc gtttctcctt
     3721 ccggcgaaag aaagctggcc caagtcgagg attactggtt ccagaaaaat cgtgtcgtat
     3781 tgaagctcgc tgattacgac gacgtcgagg ctgcgaaaca gttggttggt tatgacttcg
     3841 ccgttcacga agccgatcga gttcctctcg atgaagacga gttttacgac tgggaactcg
     3901 aaggctgcac ggtgaaggtc ggcgacaaaa gtatcggccg cgttcagtca gtgatgaaga
     3961 caggcggcgc cgagatactg gtgatcacgg atggcgcggg gaaggaacaa cttgttccac
     4021 tggctgccga gatcgttgtc aacatagaca cggcggcgcg ggtgattttg atagacccac
     4081 ccgaaggctt acttgatttg tagatctttg agccgtaaga cagacattct tgtctgtcag
     4141 aagcgacagg ctggaaagcc tgtcctacac gaaatgttac gcttcgatat catcacgatc
     4201 ttccccgagt ttttccgcga gacaatcgac tgcgggatcg tgagaagggc gcgtaatgcc
     4261 gggctggtgg aaatagccgc tcacgacttg cgtcagtgga ccacggacaa acatcacgtc
     4321 gtcgatgatc ggccgtttgg tggcggcgac gggatggtgc tgaaacccga accgatcttt
     4381 gcgggtgttg aagcactgac gggcgccgcg cggcgtgaag atttgccgtc gcacacgcgc
     4441 gtggttctgt tgtccgccca gggcgaagtt ttttcgcagg cgctggccca ggagctagca
     4501 gcagaggggg cacagagcga ttcgcggatc gtgct
//
LOCUS       DASTEY010000309         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1358004, whole genome shotgun sequence.
ACCESSION   DASTEY010000309 DASTEY010000000
VERSION     DASTEY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358004"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /locus_tag="VFO72_05405"
     CDS             complement(<1..1792)
                     /locus_tag="VFO72_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9478757.1"
                     /translation="MSRSIPTVQPAIDPVCGMTVNPATAAGSFEYNGQTYFFCSQHCL
                     HKFRETPERFLSAAPSAVAHAHHEMPPPPAKGEAIEYTCPMHPEIVRSAPGFCPICGM
                     ALEPRTATVEEDNAELNDMKRRFWVSVVLSLPLFLIAMSEMVPGDPARTLLGPRTSTW
                     VSLLLATPVVLWAGWPFFVRAWYSLVNRSLNMFTLIGLGVAVAYVYSVIAAVMPQIFP
                     ASFRDHMGNVPVYFEAAAVITTLVLLGQVLELKARSSTGAAIRSLLGLAPKTARRIGP
                     DGSEQDVPLDDVMKSDRLRVRPGEKIPVDGIVLEGNSSVDESMVTGEPIPIEKQKDDR
                     VIGGTINGNGTLVMQAERVGSETLLAQIVQMVAEAQRSRAPIQRLADVVSGYFVPIVI
                     LVAIITFVVWFTIGPEPRAAFAIINAVAVLIIACPCALGLATPMSIMVATGKGAQGGV
                     LFKNAEAIEILREIDTLVVDKTGTLTEGKPKLVTVAASSDYSEQELLRLSASLERASE
                     HPLAAAIVSGAAQRGIELVNVDAFESITGKGVKGDVGSRSVALGNRAFLDEAGIDAAE
                     LVARAEQYRAEAQTVMFVIVDGRPAGLLGVA"
     gene            complement(1785..>2132)
                     /locus_tag="VFO72_05410"
     CDS             complement(1785..>2132)
                     /locus_tag="VFO72_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478758.1"
                     /translation="EDMAPKFGRGMGDSTEKDRNTSNIVGPLEQQQHRHEASPGDESQ
                     KKRVPGYPQDMIMIMDKQVAKPHTYGLPAGWTASMMGMMTLVRVLTPEKYDEVQRRIR
                     EGIVEAPQGHKHE"
BASE COUNT          446 a          658 c          568 g          460 t
ORIGIN      
        1 cggcaacacc gagtagaccc gccggcctgc catcgacgat cacaaacatc actgtttgag
       61 cttctgcgcg gtattgttct gcgcgtgcaa cgagttccgc cgcatcgatg ccggcttcgt
      121 cgagaaaagc acgatttcca agcgcgacgg agcgagagcc gacgtctcct ttgacgccct
      181 ttccggtgat cgattcgaat gcgtcaacgt ttaccagttc gataccacgc tgcgctgcgc
      241 cggaaacgat cgcggccgcc aaaggatgtt cgctggcacg ctcgagactt gctgacagtc
      301 gcaacagttc ctgttcgctg tagtcggacg acgctgcgac agtcaccagt ttcggtttcc
      361 cttcagtcaa cgtgccggtc ttatcgacaa ccagagtgtc gatctcgcgc agtatctcga
      421 tcgcttccgc gttcttgaaa agaactccgc cttgcgcgcc ctttccggtc gcgaccataa
      481 tcgacatcgg cgtcgccaat ccgagtgcac atggacaagc gatgatgagc accgcaacgg
      541 cattgatgat cgcgaaagcc gctcttggct ctggaccaat ggtgaaccaa acgacaaagg
      601 tgatgattgc aaccagaata actataggaa cgaaataacc tgaaacgacg tccgccagcc
      661 gctgaatcgg cgcgcggctg cgctgcgctt ccgcaaccat ctgcacgatc tgcgccaaca
      721 acgtctcact gccgacacgt tcagcctgca taactaatgt gccgttgccg tttatcgtcc
      781 cgccaatgac gcgatcatcc ttctgtttct cgatcgggat cggttcgccg gtaaccatcg
      841 actcatcgac ggaggagtta ccttcgagca cgattccgtc caccgggatc ttttcaccgg
      901 gcctgacgcg caggcgatcg gacttcatca cgtcatcaag cggcacatcc tgctcactgc
      961 cgtctggtcc aatgcggcgc gccgtcttgg gtgctaatcc aagcagcgac ctgatcgccg
     1021 cgccggttga gctgcgagct ttcaattcca atacctggcc cagcaggacc agcgttgtaa
     1081 ttaccgcagc agcttcgaag taaaccggca cgttgcccat gtgatcgcgg aatgacgcag
     1141 gaaagatctg cggcataacg gcggcgatca cgctatagac gtaagcgaca gcgacgccga
     1201 gtccgatcag cgtaaacata ttcagactgc ggtttacgag tgaataccac gcccggacaa
     1261 agaacggcca cccggcccaa agcacgaccg gtgtcgcaag cagcagcgaa acccacgttg
     1321 acgtgcgtgg tcccaacagc gttctcgctg gatcgcccgg gaccatctcg ctcatcgcga
     1381 tgagaaagag tggcagcgaa agcaccacag agacccagaa gcgtcgcttc atgtcgttga
     1441 gctctgcgtt gtcttcttcc acggttgcag tgcgtggctc gagcgccatg ccgcagatcg
     1501 gacagaagcc cggcgcactg cgcacgatct cggggtgcat cgggcaggtg tattcgatcg
     1561 cttcgccctt tgccggcggc ggcggcatct catggtgagc atgcgcaact gccgaaggtg
     1621 ctgccgatag aaagcgttcc ggtgtctccc ggaacttatg cagacagtgc tggctgcaga
     1681 agaagtacgt ctggccgttg tattcaaaag atccggcggc tgttgctgga ttcacagtca
     1741 tcccgcaaac cggatcgatc gctggttgaa ctgtcggtat tgacctactc atgcttgtgc
     1801 ccctgtggcg cctcaacaat accttcacgg attcgtcttt gcacttcgtc gtacttctca
     1861 ggcgtcaaca ctcttacgag cgtcatcatt cccatcatag acgcggtcca acctgctggc
     1921 agaccatatg tatgcggctt cgccacctgt ttatccatga tcatgatcat gtcctgcgga
     1981 tagccgggca ctcgcttctt ctgcgactcg tctccgggac tcgcctcatg gcgatgttgc
     2041 tgctgctcga gtggtccaac aatattagaa gtatttctgt ccttctcagt actgtcgccc
     2101 atgccgcggc caaacttcgg cgccatgtcc tc
//
LOCUS       DASTEY010000310         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_616324, whole genome shotgun sequence.
ACCESSION   DASTEY010000310 DASTEY010000000
VERSION     DASTEY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616324"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VFO72_05415"
     CDS             complement(<1..1068)
                     /locus_tag="VFO72_05415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9478759.1"
                     /translation="MLGKTLRNYRITEKLGVGGQGAVYKAVDTKLGRTVVIKVIPPEL
                     SSRETNLKRFEREARLASSLDHPNICTIFDLDEQDGTHFIAMQYVDGKNVRQLVAGRP
                     LELKTALLIGLQVADALAAAHSRGIIHRDIKSGNVMVTSSGQVKVLDFGLAKLLDDTK
                     AATSGIHHTELTEVGVPYGTATYAAPEQARGDRVDKRADIFSLGVLLYEMLSGTWPFR
                     GKTTIDVRHAVLHDSPRPIAEVRGSPIPLRLQQIIDRALAKEPKDRFQNMDDFRNELR
                     QVLHELDSAVAGYQGVTPEPPRHLHGANPVSRAVRWFKSITRTESPTSAPNLTTSSKH
                     GIHETPFTTVTDQEKKSLAILC"
     gene            complement(1084..>2001)
                     /gene="rfaE1"
                     /locus_tag="VFO72_05420"
     CDS             complement(1084..>2001)
                     /gene="rfaE1"
                     /locus_tag="VFO72_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET9478760.1"
                     /translation="VLGDVMLDEFVWGDVTRISPEGPVPVVDVRRESVHLGGAANVLA
                     NLIALGAHGWVVGVVGNDAAGERLRDELRQLNSAGDGGVIVDETRRSTTKTRIVAHSQ
                     LVVRADRESRTPVNANVEERIVARLKEKLEEADAFVVSDYDKGVITPAILREVLPFAY
                     ERVPVLVDPKLRNFNSYRPATLITPNHLEALRMSDTEDHSDDGSHEAARVIQQKLGCD
                     AVLITRGDRGMMLLEAGGQPVYVETAAREVYDVTGAGDTVIATLAGALASGATMLEAA
                     TLANHAAGIVVGKVGTATVTASELLATFE"
BASE COUNT          372 a          608 c          588 g          433 t
ORIGIN      
        1 acataagatc gcgagactct tcttctcctg gtccgtaaca gtcgtgaacg gtgtttcgtg
       61 gatgccgtgc ttcgaagacg tcgtgaggtt tggcgcggag gtcggcgatt ctgttcgtgt
      121 gatcgactta aaccagcgca ccgcgcgcga taccggattg gcgccgtgca gatggcgtgg
      181 tggctcaggc gtgacgccct ggtaacccgc gacggccgag tcaagttcgt gtagcacttg
      241 ccgcaactcg ttgcgaaagt cgtccatgtt ttgaaaacga tctttcggtt ctttagccag
      301 cgcgcgatcg atgatctgtt gcagccgcag cgggatcgga ctgccgcgta cttcggcgat
      361 cggccgcggc gaatcgtgca gcactgcgtg acgcacatcg atcgtcgtct tgccgcgaaa
      421 cggccacgtg ccgctgagca tttcgtaaag aagtacgccg agcgaaaaga tgtcggcgcg
      481 tttgtcgacg cgatccccgc gcgcctgctc gggcgcggcg tacgttgccg ttccgtacgg
      541 aacaccgacc tcggtgagtt cagtgtgatg aatgccggaa gtcgctgctt tggtgtcgtc
      601 gagaagtttt gccaaaccga agtcgagcac tttcacctga ccggaagagg tgaccatgac
      661 gttgccggat ttgatgtcgc gatgaatgat gccgcgactg tgtgccgcgg ccagtgcgtc
      721 ggccacctgt aaaccgatca gaagcgcggt tttcaattcg agtggccggc cggccaccag
      781 ttgccgcacg ttcttgccgt cgacgtattg catggcgatg aagtgcgtgc cgtcctgttc
      841 gtcgagatcg aagatagtgc agatgtttgg atggtccagc gaagaggcaa gacgggcttc
      901 tctttcgaag cgtttgaggt ttgtttccct cgagctgagc tcgggtggaa tgactttgat
      961 aacaaccgta cgtcccagct tagtatcgac tgctttgtag accgctccct gaccgcccac
     1021 gccgagcttt tccgtgatgc gatagttgcg aagagtcttg cctaacattg gtccgtggtc
     1081 tgctcattcg aaagtggcga gcagctcgct cgcggtaacc gttgctgttc ccactttgcc
     1141 aacaacgatt cccgcggcgt gattggcgag cgtcgcagcc tccagcatag tggcgccgga
     1201 agcgagtgcg cccgcgagtg tcgcgatgac cgtgtcgccg gcgcctgtga cgtcataaac
     1261 ttcgcgcgcg gcggtttcga cgtaaaccgg ctgtccgccg gcttccaaca gcatcatgcc
     1321 gcgatcgccg cgagtgatga gaaccgcgtc gcaaccgagt ttttgttgaa tgacccgggc
     1381 cgcttcgtga gatccgtcgt cggaatggtc ctccgtgtcg ctcatgcgca acgcttccag
     1441 gtgattcgga gtaatcagcg tcgccgggcg gtacgaattg aagttgcgca acttcgggtc
     1501 aaccagaacc ggaacacgct cgtacgcaaa cggcagcacc tcgcgcagta tcgccggcgt
     1561 gatcacacct ttgtcgtaat cggaaacaac aaacgcatcc gcctcttcta gcttctcctt
     1621 cagtcgcgcc acgatccgtt cttcgacatt tgcgttgacc ggcgtgcgcg attcacgatc
     1681 cgctcgcacc accaactgac tgtgcgcgac aatgcgcgtc ttcgtcgttg aacgccgtgt
     1741 ctcgtcaaca atcactccgc cgtcgcccgc ggagttcaat tgtcgcaact cgtcacgcag
     1801 tcgttcgccc gccgcatcgt ttccgacaac gccaacaacc caaccgtgcg cgccgagcgc
     1861 gatcaagttc gccagcacgt ttgctgcgcc gcccagatgg accgactcgc gccggacgtc
     1921 caccaccggc accggccctt cgggagagat tcgcgtcacg tcgccccaaa caaactcgtc
     1981 gagcatcacg tcgccaagca c
//
LOCUS       DASTEY010000311         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1390951, whole genome shotgun sequence.
ACCESSION   DASTEY010000311 DASTEY010000000
VERSION     DASTEY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390951"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..804
                     /locus_tag="VFO72_05425"
     CDS             109..804
                     /locus_tag="VFO72_05425"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HET9478761.1"
                     /translation="MAKTLGLIGGTGPESTIEYYRLLVAKYREQADGNSPDVIINSVN
                     LKNMIEWMTAGELGKVTDYLSAAIDKLRLAGADLAALTANTPHIVFDELQRRSALPLI
                     SIVQATCDKARTLGLKSVALFGTRFTMQAPFYPAVFSRAGIKLVLPNDEEQNYIHEIY
                     MGELLKDVFLPETRTRLLTIADTMRGRDGIEALILGGTELPLLLRDEEHSGMPLLDTT
                     RIHVERLIAEMLS"
     gene            1046..>2080
                     /locus_tag="VFO72_05430"
     CDS             1046..>2080
                     /locus_tag="VFO72_05430"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HET9478762.1"
                     /translation="MKPFRSDRELKRYLQEAAEKYRRARAQETQAAVMVSSAETVAVS
                     SQGADESVTNTQHEGVDEGGIVKLHGNHLVILRRGRLFTIAIGNGALRPVSSIDAFGP
                     DLDPDQAWYDEMLISGDTIAVVGYSYKRSGTEVGLFNIDSQGQLSYRSTYTLRSNDYY
                     SSRNYASRLIGSKLIFYNGLYFNPNTDDPFASFPAVRKWHKGGTDEDFRRIVSYSRVY
                     RPAGDFDVQYGVTLHTVTTCDLARGFDCQATTVVGPHGNVFYVSPESVYVWAVASAWY
                     SDTRGQSFLFRMPLDGSGPSALRVSGGPVDQFSFLQSKDHHLNVLVRAESKGDGMWGA
                     EVSQGEVALMR"
BASE COUNT          466 a          620 c          546 g          448 t
ORIGIN      
        1 tcgtgggctg gttcgataag tggatcatgg ttgtggcgaa acccgaatat gacatcggcg
       61 aggtgacacg aaccactacc actcaacaat aaggatgcgt ttaatcctgt ggctaaaaca
      121 ctcggactca tcggcggcac cggtcccgaa tcgaccatcg agtattaccg tctgcttgta
      181 gcgaagtatc gcgagcaggc ggacggtaat tctccggatg tgatcatcaa cagcgtcaac
      241 ctgaaaaaca tgatcgagtg gatgacggct ggtgaacttg ggaaggtcac cgactatttg
      301 tccgcagcga tcgacaagct gcgccttgca ggcgcggatc tcgctgcatt gacagccaac
      361 actcctcaca tcgtcttcga tgaattgcaa cggcggtcgg cactgccgct aatcagtatc
      421 gtccaagcga cctgcgacaa ggcccggacg ctcggactaa aatccgtggc actcttcggc
      481 acgcgcttca caatgcaggc gccgttttac ccggctgtct tctcgcgcgc cggcatcaag
      541 ctcgtgttgc ccaatgacga agagcaaaac tacattcacg aaatctacat gggtgaactg
      601 ctcaaggatg tcttcctccc cgaaacgcgc accagactcc tcacaatcgc cgacacgatg
      661 cgcggacgcg acggcatcga ggccctcatt ctgggcggca ccgaactgcc cctgctgctg
      721 cgcgacgaag aacacagcgg catgccttta ctcgacacca cacgcatcca cgtcgagcgc
      781 ctcatcgcgg agatgttgtc ctaatactgg acgtgcaaga cctgtctgaa ctcaccagca
      841 tggcaaccta aatcgcctaa tagttatcaa acggtccaac gtctccctcc caactggcct
      901 gcaagtcatt agaaccgtcc tagttctttt cgccatgaga acactcttcc cgtcagcact
      961 cgccgccgct ttggttcttt gtgtctcgcc ttgtgtaact acaaattctt cagttaacgc
     1021 agctgttgat cgcacccgca agaagatgaa gccattccgt tccgaccgtg aactgaagcg
     1081 ttacctgcaa gaagcggccg agaaataccg ccgcgctcgc gctcaagaga cccaagccgc
     1141 ggtcatggtc agcagcgccg agaccgtagc tgtgagttcc cagggggcag acgagtcggt
     1201 tacgaacacg cagcacgaag gcgttgatga aggcggtatc gtcaagctcc acggcaatca
     1261 tcttgtgatc ctccgccgcg gccggctctt caccattgca atcggtaacg gcgcattgcg
     1321 tcctgtttcg agcatcgatg cttttggacc ggacctcgat ccggaccagg cgtggtacga
     1381 cgaaatgcta atctccggcg acactatcgc cgtcgtcggc tacagttata aacgtagtgg
     1441 caccgaagtt ggactattca acatcgactc ccagggtcaa cttagttatc gctcgaccta
     1501 taccctgcgc tcgaacgact actattcttc acgcaattac gcgagccgct taatcggcag
     1561 caaactcatc ttttacaatg gcctctactt caatccgaac accgacgatc cgtttgcatc
     1621 gtttcccgcc gtgcgcaagt ggcacaaggg aggtacggat gaggattttc gccgcatcgt
     1681 ctcctacagc cgagtctatc gaccggcagg cgacttcgac gtccaatacg gcgtcacgct
     1741 gcacacggtg actacgtgcg atctcgcacg gggctttgat tgtcaggcga caacagtggt
     1801 tggaccacac ggaaacgtat tctacgtctc acctgaatcc gtttatgtct gggccgttgc
     1861 gtccgcctgg tacagcgata cccgcgggca gtcgtttctc tttcgaatgc cacttgacgg
     1921 ttccggcccg agcgcgttgc gcgtgtcggg tggtccagtg gatcagtttt cgttcctgca
     1981 gagcaaagat caccacttga acgtgctcgt gcgtgccgag tcaaaaggcg atggcatgtg
     2041 gggagccgaa gtgtcacaag gagaggtcgc gttgatgcga
//
LOCUS       DASTEY010000312         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1028441, whole genome shotgun sequence.
ACCESSION   DASTEY010000312 DASTEY010000000
VERSION     DASTEY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028441"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFO72_05435"
     CDS             complement(<1..397)
                     /locus_tag="VFO72_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9478763.1"
                     /translation="MKLTFLGTGTSTGVPSIGCECDTCTSDDPRDKRLRVSVIIEHAG
                     RTILVDTSSDFRQQALRFGLKRLDAVLITHCHADHIFGLDDIRPLNFRFGALGVYANE
                     RAWVDIKRIFKYIFEPSHFGGGLPQVIPYT"
     gene            complement(472..1005)
                     /locus_tag="VFO72_05440"
     CDS             complement(472..1005)
                     /locus_tag="VFO72_05440"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET9478764.1"
                     /translation="MSNDQPTYKVTDRRLFNADGSPRDLPPEEKPEPKPETVTATAPT
                     ATAQGAQPEAATTVPPAESTGELGEEFTAEELADARDPASFISFMMSIASNAASALGM
                     MEHPVTHEREVDVELGKHWIDVLGMLQKKTAGNLSPKEKQMLESLLSDLRMQYVSLVN
                     AAPRAQRFSGNDITGGR"
     gene            1060..1890
                     /gene="mazG"
                     /locus_tag="VFO72_05445"
     CDS             1060..1890
                     /gene="mazG"
                     /locus_tag="VFO72_05445"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET9478765.1"
                     /translation="MFNDLVNLMERLRSPNGCPWDREQTYATLAPMLLEEAYEAFEAV
                     EEAREGRPAELRDELGDLLFQIVFYAQVAKERGEFAIDEVIAAIHDKMVRRHPHVFGD
                     VTAEDSATVLLNCETMKAEERRAAGKTEDTKTSLLDGVSSKAPALMEAHQLSTKAARV
                     GFDWKSVDDIFDKLQEEIEELRRAIREHENLKDEANHARVREEIGDLLFAATNIARHM
                     QVEPEAALKLTNRKFRRRFEYIETSLHDRNQAFDQTSIEELEDLWQQAKATDHTAVNR
                     "
     gene            complement(1880..3283)
                     /locus_tag="VFO72_05450"
     CDS             complement(1880..3283)
                     /locus_tag="VFO72_05450"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9478766.1"
                     /translation="MNKWRKSLIAWVTLIAIMAVPLSIAAQTQLKYHSNRFSIQDDVK
                     LGRQAAQEAEQQFPLLSDNEVRSYVESIGERLVAAIPSQFQHPEFDYYFKVVNARDIN
                     AFALPGGPMYVNRGMIEAARTEGEMAGVMAHEISHVALRHGTAQATKGQKYGTLAAIA
                     GIAGTIFGGPGIGQLAQAPFAVYLLKFSREYETEADILGAQILARAGYDPRDLANMFR
                     TIEQQGGGSSGGFLSSHPSPKDRYARINREAQLLRINTATRDTREFARVKERLRGYPT
                     APTMAEIARSGQRYPTGENTSYPNRLPTGRVEYPSSRYQNVSVFNGGVRVNVPSNWRQ
                     INEGSSVWFAPEGGYGSYNGQAVFTHGANLGVAQTSNRNLQRATDEIISSFAQANRNL
                     RASGGYQRTTIDGRQALWTNLTNVNQATGQREAIRLITTQLSNGQVLYMVAVAPQGER
                     NFDNAFQNMLRSIRIND"
     gene            complement(3413..4090)
                     /locus_tag="VFO72_05455"
     CDS             complement(3413..4090)
                     /locus_tag="VFO72_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET9478767.1"
                     /translation="METKKSKYDTNPLDPDVERKAEDSWGGGAPTQQVGGATRDVGHT
                     PNEEARQNVYSEAPTRRYDNPPLEASYPSVFVPPTYAPPNQYQQAAPNAYHSTIPNPP
                     TSRPVIGLGIPEKWAVLLAYAPWVGIVASLVELFLASRRETRVRFHASQALALHIVIL
                     IISTIFGVIGTLSGSDFGGILFKIAATVFLVISMIRAWRGEPHRIAPLAEPAQWFNEH
                     IEPRSQG"
     gene            complement(4127..4549)
                     /locus_tag="VFO72_05460"
     CDS             complement(4127..4549)
                     /locus_tag="VFO72_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9478768.1"
                     /translation="MSTIRLFREHIDAMIAHAREAAPAECCGLIGGVSDKDARTIYRL
                     RNVSANPKLGYEAAPEDLFTAQRQMRERGEDLLAIYHSHPRADEPSPSETDVRLAYYP
                     SATYLIVGLGGTEATVRAFSISEREHRWNQVEYEIAGE"
     gene            complement(4632..>5496)
                     /locus_tag="VFO72_05465"
     CDS             complement(4632..>5496)
                     /locus_tag="VFO72_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET9478769.1"
                     /translation="GTEFPPFASALTSGGHVKGIVVPGGANLSRKMLDELQEFAKRYG
                     AGALPWIKLGDEISSSLLKALGPAAVSRVADLAGAKRGDAVLIVAGKPKVVAASLGAL
                     RNEVAKRENLVPARVYAPLWVTDFPMFEFHEEDGRYYSMHHPFTSPVDEDLQLLERAV
                     EVGETNLLAQIRTKAYDPVINGVEMASGSIRIHRRDVQRLVFKALGMTTEEARQRFGF
                     FLDALEYGTPPHGGIAPGIDRLMMVLAGTDNMRDVIAFPKTASAADLMIDAPGAVDPK
                     QLEELHLRIVE"
BASE COUNT         1188 a         1562 c         1550 g         1196 t
ORIGIN      
        1 ccgtgtaagg aatcacctgc ggcagtccgc cgccgaaatg tgatggctca aagatgtatt
       61 tgaaaatgcg cttgatatcg acccacgcgc gctcgttcgc gtaaacgcca agggcgccga
      121 aacgaaagtt gaggggccgg atgtcatcga gaccaaaaat gtgatcggcg tgacagtgcg
      181 tgatcagcac tgcatcgagt cgcttgagtc cgaagcgcaa cgcttgctgg cggaagtctg
      241 aggaagtatc taccaggatg gtgcggccgg catgctcgat aatcactgaa acgcgcagac
      301 gtttgtcccg cggatcgtcc gaggtacagg tgtcacattc gcaacctatc gacggcacgc
      361 cggtcgacgt ccctgtaccg aggaaagtga gtttcatgct ggttgagctt tgttctgagt
      421 actttggtct ttgtgacttc tgacaaaagt acagagcaca aatctcgacg tttatctccc
      481 gccggtgatg tcattgccgg aaaagcgttg cgcgcgtgga gcggcgttca cgagcgagac
      541 atactgcatg cgcaaatccg acagcaaact ctcgagcatc tgcttttctt ttggacttaa
      601 gtttcccgca gtcttctttt gcaacatgcc gagaacgtcg atccaatgct tgcccagttc
      661 gacatcgacc tcacgctcat gggtgaccgg gtgctccatc attccgagcg cggacgcggc
      721 gttggaagcg atcgacatca tgaagctgat aaaactcgcc gggtcgcgcg catccgccag
      781 ttcttctgcc gtgaactcct cgcccagctc gcccgtcgat tcagccggcg gcacagtggt
      841 cgccgcttca ggttgcgctc cttgggcagt cgcagttggg gcagtcgccg ttaccgtttc
      901 cggctttggt tcaggtttct cttccggagg aagatcgcgc ggacttccgt cagcattaaa
      961 cagccgccga tccgttactt tgtaagttgg ttgatcgtta ctcataactc taattgtgaa
     1021 tttagcatga tggtagcata gactactgcc atggccacaa tgttcaacga tctcgtaaat
     1081 ctgatggaac gcctgcgctc gccaaacggc tgcccgtggg accgcgaaca gacctacgcc
     1141 acgctcgcgc cgatgctgct cgaagaggcg tacgaagctt ttgaagcggt agaggaagca
     1201 cgagaaggcc gccccgcgga actgcgcgac gagctgggtg atctcctttt ccaaatcgtc
     1261 ttctacgcgc aggtcgcgaa ggaacgcggc gagtttgcta tcgacgaggt aatcgcggcc
     1321 attcacgaca agatggtgcg gcgccacccg cacgtctttg gcgacgtcac agccgaagac
     1381 tcagccacag tcctgctgaa ctgtgaaacg atgaaagcag aagagaggcg cgcggcggga
     1441 aaaacggagg acacgaagac ttctttgctc gatggcgttt cgtcgaaagc accggcgttg
     1501 atggaagctc atcaactctc aaccaaagcc gcacgcgtcg gcttcgactg gaagtccgtc
     1561 gacgatattt tcgacaagtt acaagaggag attgaagagc tgcgacgcgc gatcagagaa
     1621 cacgagaacc tgaaagacga agcaaatcac gcgcgcgtgc gagaagagat cggcgatttg
     1681 cttttcgctg cgaccaacat cgcgcgacac atgcaggtcg aacccgaagc ggctttgaaa
     1741 cttacgaatc ggaagtttcg ccgccgtttc gagtacattg aaacctcgct gcacgatcgc
     1801 aaccaggcgt ttgatcaaac ctcaatcgag gaactcgaag atctgtggca acaagccaag
     1861 gccacagatc acaccgccgt caatcgttaa tgcgtatcga gcgcaacatg ttctgaaacg
     1921 cgttgtcaaa gttgcgttcg ccctgcggcg caacagcgac catgtaaagc acctggccgt
     1981 tgcttaattg cgtagtaatc aacctgatcg cctcgcgttg ccctgtggcc tggttgacgt
     2041 tagtcaaatt agtccacagc gcctgccggc catcgatcgt ggtgcgctga taaccaccgc
     2101 ttgctcgcaa attacgattt gcctgagcga aactgctgat tatctcgtca gtggcgcgct
     2161 gcaagttgcg attgctggtc tgcgctacgc ccaaattcgc accatgcgta aacaccgcct
     2221 ggccgttgta cgacccataa ccaccctcag gcgcgaacca cactgaactg ccctcgttaa
     2281 tctgccgcca gttggacggc acgttcactc gcacgccacc attaaagact gagacgtttt
     2341 ggtaacgcga cgaaggatac tcaacacggc ccgtcggcaa gcggttcggg tagctggtat
     2401 tttcgccggt cggataacgc tgaccgctgc gagctatttc ggccatcgta ggcgcggtgg
     2461 gataaccacg cagccgttcc ttgacccgag caaactcgcg cgtatcgcga gtggcagtat
     2521 taattcgcag taactgagct tcccgattga tgcgcgcata acgatccttc gggctgggat
     2581 ggctactcag gaaccctccg gaggaaccac caccttgttg ctcgatcgtg cgaaacatgt
     2641 tcgccagatc gcgcggatcg taaccggctc gcgccaggat ctgcgcgcct agtatgtcgg
     2701 cttcagtttc atactcgcgg ctgaacttca gaaggtagac cgcaaatggc gcctgggcca
     2761 actgccctat gccgggtcca ccgaagattg ttccggcaat accggcaatc gcggcgagcg
     2821 ttccatattt ttgtcccttg gtcgcctgag ccgtgccgtg acggagtgcg acgtggctga
     2881 tctcgtgagc catcacaccg gccatctcgc cttcagtgcg ggccgcttcg atcattccgc
     2941 ggttgacgta cattggtcca ccgggaagtg cgaacgcgtt tatgtcgcgc gcgttgacga
     3001 ccttgaaata gtaatcaaat tcgggatgtt gaaattgcga cggaatggca gccaccaagc
     3061 gctctccaat actttccacg tagcttcgaa cttcattatc gctaagcagc ggaaactgtt
     3121 gttctgcctc ttgcgccgcc tgacgtccaa gtttgacgtc gtcttgaata ctaaatctgt
     3181 tgctgtgata tttgagctgc gtctgagccg cgatcgacag cggcactgcc atgatcgcta
     3241 tcagcgttac ccatgcaatt aacgattttc gccatttatt cataccctaa cctccgacgg
     3301 tagctgtaac ccttttccga gacaaatgca ccaaaacgaa acagaatcgg cccaaatatc
     3361 gggttgcggg cgaccttcta gccattaatg caatgatggt gccaatgtgg ggttaaccct
     3421 gactacgcgg ctcaatgtgt tcgttaaacc attgcgccgg ttcggcaaga ggagcaatac
     3481 gatgcggctc accgcgccat gcgcggatca ttgagatcac cagaaagaca gtcgcggcga
     3541 ttttgaagag tattccaccg aagtcactgc cactgagagt gccaatcacg ccgaagatag
     3601 tagaaatgat gagaatcacg atatgcagcg ccaatgcctg cgaggcgtga aagcggactc
     3661 tcgtttcgcg gcgcgaagca aggaacagtt cgaccagcga ggcgacaatg ccgacccacg
     3721 gagcataggc cagcaatacc gcccatttct cgggtatgcc gaggccgatc actggccgtg
     3781 atgtgggcgg attcggaatc gtgctgtgat aggcatttgg agccgcttgt tgatactgat
     3841 tcggtggcgc gtaagttgga ggcacgaaaa ccgacggata cgacgcctca agcggtggat
     3901 tgtcgtaacg gcgagtcggc gcttctgagt agacgttttg ccgcgcttcc tcgttaggag
     3961 tgtgcccgac gtcgcgcgtc gcacctccga cctgctgcgt cggtgctccg ccaccccacg
     4021 agtcttccgc cttgcgctca acgtcgggat cgagggggtt ggtgtcgtat ttgctcttct
     4081 tcgtctccat tctcagccgc tctccaaagc tgggtcccgg gaaatcttac tcgcccgcga
     4141 tctcatactc aacctgattc caacggtgtt ctcgttctga aatagaaaac gccctcacgg
     4201 tggcttcggt tccaccgaga ccgacgatca ggtaagtcgc tgaagggtaa taagcaaggc
     4261 gaacgtcagt ttccgacggg cttggctcgt cggctcgcgg gtgtgagtga tagatcgcga
     4321 ggaggtcctc gccgcgctcg cgcatttgcc gctgggcggt gaagaggtct tcgggcgccg
     4381 cttcgtagcc gagtttggga ttcgcgctga cattgcggag gcgataaatc gtgcgggcat
     4441 ccttatccga gacgccgcca atcagtccgc aacattcagc gggagccgct tcgcgagcat
     4501 gagcaatcat tgcgtcgatg tgttcgcgaa agagccgtat ggttgacatt ttgctctcgg
     4561 ccacagataa acacggacgg acacggaatt cattagcgcc tgtctgtgtt acatccgtgt
     4621 aagtccgtgg cttactccac gatacgcaga tgcagctctt ccaattgctt cggatcgacc
     4681 gcgcccggcg cgtcgatcat caggtccgcc gccgatgccg ttttgggaaa cgcgatcacg
     4741 tcgcgcatgt tgtcagttcc ggcgagcacc atcatcagac ggtcgatgcc tggggcgatg
     4801 ccgccgtgtg gcggcgtgcc gtactcgagt gcgtcgagaa aaaagccaaa tcgctggcgc
     4861 gcttcttcgg tagtcatgcc gagtgctttg aagacaagtc gttgcacgtc gcggcggtga
     4921 atacgaatcg aaccgctcgc catttcgacg ccgttgatca cagggtcgta agccttcgtg
     4981 cgaatctgcg cgagcaggtt tgtttcacca acttcgacag cgcgctcgag taactgcaag
     5041 tcttcgtcga ctggcgacgt gaagggatgg tgcattgagt agtaccggcc gtcctcttcg
     5101 tgaaactcga acatcggaaa atctgtgacc cagagcggcg cgtagacgcg cgccggaacc
     5161 aggttctcgc gtttggcgac ttcgtttcgc agggcaccga gacttgcggc cacgactttt
     5221 ggttttccgg cgacaatcaa aacggcatcg ccacgcttcg cgccggcaag atcggccacg
     5281 cgagacaccg cagctggtcc cagtgctttt agcagcgacg acgagatctc atcgccgagt
     5341 ttgatccatg gcaacgcgcc cgcaccgtag cgcttggcaa actcctgcaa ctcgtcaagc
     5401 atcttgcgtg aaaggttagc gccgccgggc accacgatgc cttttacgtg tccgcccgaa
     5461 gtcaatgccg aggcaaacgg tggaaattcc gtgcct
//
LOCUS       DASTEY010000313         5037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1094644, whole genome shotgun sequence.
ACCESSION   DASTEY010000313 DASTEY010000000
VERSION     DASTEY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094644"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /locus_tag="VFO72_05470"
     CDS             complement(<1..2247)
                     /locus_tag="VFO72_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9478770.1"
                     /translation="MADSPAPGESLVRKEPELPANDPIVSRSTSGWMLVSALLMTISI
                     AWALYDEAFGQRPWKGMQKEFVTRYTRYLDSIRANAGKSEAEIKESPEYQQLDAEYKA
                     AEEQILPETKQIDAEVARVQKRLDAITDPFQNQRGRLTVINYYVETSEGSAKDRYKRQ
                     AEEKKQELVELKLPNDDGNSVSTQRLNYAQLEKLYDELREQKAQGLARKAELLKAPSE
                     LRKKRDDYLRNQLIGLGPAAIDGLKTKMANYDYSILGHQISVTEYEIVDRCEVCHAGI
                     REPLELTPASLAPDKPGGEPDSLARAFVSHPNREIFQVHSPEKFGCSSCHWGNGRATT
                     SETKGHGRHRFWLWPMFEKENTQAGCQQCHAHDRVTDGAETLNLGRDLFAERGCMGCH
                     RYEGFDRETDALNAARQQIGQLEDQIIGNEKQIRADTQRSGEVEDNDEAQRLLAHAES
                     LRVTNSLLAARIDQLNLQTKYLMQDQKKVGPNLKDVRVKLRKEWVPLWLENPQGFRPG
                     TKMPTFWRFAAPMEGRVSMRDKDGAEQIQAIAAYLWQDSFDIKLPEQQRGDAAHGKQL
                     FESRGCLACHSIGEGDSKIGGDFAANLQRVGEKANFDYIVRWIHNPRERVAPYCPKEK
                     RDLTHEDYAKKGLPFVFDTELHSRCPNDGAELQVQNMTVMPNFRLSEQDSRDIATYLF
                     SLSSPPSYASAAFMDDPNVKEKGRLLIKQYGCAGCHEIRGFEDEQRIGKELTVEGATP
                     IERLDFALQ"
     gene            complement(2270..3385)
                     /locus_tag="VFO72_05475"
     CDS             complement(2270..3385)
                     /locus_tag="VFO72_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478771.1"
                     /translation="MRRFVNKWTVLVIGLLIVGVWAYNVQDWHQLWVISTAPDNVPIV
                     GMLFLVPFFTWMGVRQAVANDRLIKQLEADPKLAKTHHRKVEPWRPGWARELHVWPYL
                     VRIEFLATVIVTVVLFVWSITLNAPLEEPANPNLTMNPSKAPWYFLGLQEMLVYFDPW
                     IAGVVMPSIIMIGLMAFPYVDSNPLGNGYYTYRQRRFALWMFGAGFLQWIVLIVIGTF
                     IRGPGWIWFWPGQTWDHNAVVFDKNVDLHDMIATSKIGQTLGLGFLAVDPFKFIFGLI
                     VVTGFFIVGGLFFHWLMTVDFKKMRLPRSLRPRELHSALFPKNEFEQKILQRTSLLQY
                     LTFQFFAVSVLLALPVKLILRLALTIKYVWVTPWFNI"
     gene            complement(3416..4339)
                     /locus_tag="VFO72_05480"
     CDS             complement(3416..4339)
                     /locus_tag="VFO72_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET9478772.1"
                     /translation="MDTDPKETKQLTTGEEAGSKSLVSRVKEGTSALKDRAIEEAKEY
                     KDPQETQLWKSIFRVSHDRSDPRNRSLAILSNVFLHLHPAKINRDAVRYPYTWGMGGI
                     TFYLFIVLTFTGVLLMFYYHPTKADAFRDILYLEHDVPFGKLLRNMHRWAAHLMVITT
                     WLHMFRVVLTGSYKRPREFNWCVGVVLLVLTLLLSFTGYLLPDDQLGFWAVTVGTNMA
                     RATPMLGSEGPFGPQLGLTAYNDVRFGLLGGSIVDSNALLRSYIWHCIGIPLVASIFM
                     AVHFWRIRKDGGISGPAPIMLESEMKGVKKK"
     gene            complement(4358..4963)
                     /locus_tag="VFO72_05485"
     CDS             complement(4358..4963)
                     /locus_tag="VFO72_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9478773.1"
                     /translation="MDSQADQISARPAGTAVNVPSQANVGAAAPAKPATVETTSDPPV
                     NKMRRRIVWATVAGSLITFFLMFVRFFLPRSILEPSSVFRIGPPSDYALGVDTKWQQQ
                     YRIWVTRTSDRLFVIYARCTHLGCTPDWKASENKFKCPCHGSGYDSEGIAFEGPAPRP
                     MDRAKVSLDAEGQIVVDVGTLYEWPKGGTNEFEDENAYIRV"
BASE COUNT         1083 a         1455 c         1345 g         1154 t
ORIGIN      
        1 ctggagtgcg aagtcgagtc gctcgatcgg cgtagcgcct tcgacggtta attccttacc
       61 gatacgctgc tcgtcctcaa aacccctaat ttcgtgacag ccggcgcaac catattgctt
      121 gatcaacagc cggcccttct ctttaacatt cggatcgtcc atgaacgccg ccgaggcgta
      181 actcggcggt gacgagagcg agaagagata agtagcgatg tcccgcgaat cctgttcaga
      241 caaacggaag ttgggcatca ccgtcatgtt ctgcacttgc agttccgcgc cgtcattcgg
      301 gcagcgggaa tgcagttcgg tgtcaaaaac aaacggcagg cctttcttcg cgtagtcttc
      361 gtgggtcaga tcgcgttttt ctttgggaca gtacggggcg acgcgttcgc gcgggttgtg
      421 aatccagcga acgatgtaat cgaagttggc tttctcgccg acgcgttgca ggttggcggc
      481 gaagtcgcca ccgatcttgc tgtcgccttc accgatcgag tgacacgcga gacagccacg
      541 cgattcgaag agttgtttgc cgtgcgcagc gtcgccgcgt tgttgttccg gcagtttgat
      601 gtcgaagctg tcctgccaca ggtacgccgc gattgcctgg atctgctcgg cgccatcctt
      661 gtcgcgcatg ctcacgcggc cttccatcgg cgcggcgaaa cgccagaatg tcggcatctt
      721 ggtgcccgga cggaagccct gcgggttctc gagccacaac ggcacccatt ccttgcgcag
      781 tttgacgcga acgtctttca ggtttggacc aacctttttc tggtcctgca tcaggtattt
      841 agtttgcagg tttagctgat cgattcgcgc ggcgagcaga ctgttggtga cacgcaggct
      901 ttcggcatgg gccaacaatc tctgcgcttc gtcgttgtct tcaacttcgc ctgagcgctg
      961 cgtatcggcg cggatctgct tttcgttgcc gatgatctga tcttcgagtt gtccgatctg
     1021 ttgccgcgcc gcgttcagag catctgtttc gcgatcgaat ccttcgtaac ggtgacaacc
     1081 catgcaacca cgttcggcga agagatcgcg gccgaggttc aatgtctccg cgccgtcagt
     1141 tacacgatcg tgcgcgtgac actgctggca gccggcctgc gtgttctctt tttcaaacat
     1201 gggccagagc cagaaacgat ggcggccgtg cccttttgtt tcgctggtgg tcgcgcgtcc
     1261 gttaccccag tgacaactcg agcaaccaaa cttctccgga ctgtggacct gaaagatctc
     1321 cctgtttgga tggctcacga atgcgcgcgc cagactgtcg ggctcgccgc ctggcttgtc
     1381 aggagcaaga ctcgcaggcg tcaactccaa cggttcgcga atgccggcgt gacacacttc
     1441 gcaacggtcg acgatctcat attcagtgac gctgatctgg tggcccagga tcgagtagtc
     1501 gtagttcgcc atcttggttt tgagaccgtc gattgcagcg ggccccaaac cgattagctg
     1561 gtttcgcaaa tagtcgtcgc gtttctttct taactccgaa ggcgctttca acagctctgc
     1621 tttccgagcc aatccctgag ctttttgttc gcgtaattcg tcgtagagtt tttcgagttg
     1681 ggcgtagttt aaccgctgcg tcgagacaga gtttccatca tcattcggca acttgagctc
     1741 aaccagttcc tgtttcttct cttcagcctg tcgcttgtaa cgatccttcg ccgatccttc
     1801 gctggtctcg acgtagtagt tgatgacggt cagacggccg cgctggtttt gaaacggatc
     1861 cgtgatggcg tcgagtcttt tctgaaccct ggcgacctca gcatcaatct gttttgtctc
     1921 cgggagaatc tgttcctcgg cggctttgta ctctgcatcg agttgctggt actccgggct
     1981 ttctttgatt tcggcttctg acttcccggc gttggccctg atgctatcga ggtagcgcgt
     2041 gtagcgggtg acgaactcct tttgcattcc cttccagggt cgttggccaa aagcctcgtc
     2101 atagagcgcc cacgctatcg agattgtcat taacagcgcg gacacgagca tccatccgct
     2161 cgtagaacgg ctcacaatcg gatcgtttgc cggtaactcc ggttcttttc tgactaatga
     2221 ttcgcctggt gctggagaat cagccactgt gttcgtccct ccgcctttct cagatgttga
     2281 accagggagt cacccacacg tacttgatcg tcaacgctaa tcgcaaaatc aacttcaccg
     2341 gcaatgcaag caacacactc acagcaaaga actggaacgt cagatactgc aagagactcg
     2401 ttcgctgaag aattttctgt tcgaactcgt tcttcgggaa aagggcgcta tgaagttcgc
     2461 ggggacgcaa cgagcgcggc aaccgcatct tcttgaagtc gaccgtcatc agccagtgga
     2521 agaagagccc gccaacgatg aaaaagccgg tgacgacaat caaaccgaaa ataaacttga
     2581 atggatcgac agcaagaaat ccaagaccca acgtttgacc gatcttgctg gtggcgatca
     2641 tgtcgtgcag gtcgacgttc ttgtcgaaca ccaccgcgtt gtggtcccac gtttggccgg
     2701 gccagaacca gatccagccg ggaccacgga taaacgtgcc gatcacaatc aaaacgatcc
     2761 actgcaaaaa tcccgcgcca aacatccaaa gcgcaaagcg ccgttggcga tacgtgtaat
     2821 aaccattacc cagcggattc gagtcgacat aaggaaaagc catcaatccg atcatgatga
     2881 tcgacggcat taccacgcct gcgatccacg gatcgaaata gacaagcatc tcctgcaagc
     2941 ccaggaagta ccatggcgcc ttcgatggat tcatcgtcag gtttggattc gcaggttctt
     3001 ccaacggcgc attaagcgtg atggaccata caaaaaggac caccgtaacg ataacggtgg
     3061 ccagaaactc gattcggaca agatagggcc aaacgtgaag ttcccttgcc catcccggcc
     3121 gccacggctc gactttgcga tggtgtgtct ttgcgagttt gggatcggct tcgagttgct
     3181 tgatgagccg atcgttagcc actgcctgac gcacgcccat ccacgtgaag aacggaacga
     3241 ggaaaagcat gccgacgatt ggcacgttgt ccggcgccgt cgagataacc cagagctggt
     3301 gccagtcttg aacgttatac gcccagacgc ccactatcag caggccaatg acaagcaccg
     3361 tccatttatt cacgaatcta cgcatatgtc cgtatcatcc gtgtttatct gtggctcact
     3421 tcttcttcac gcccttcatt tcagactcga gcatgatcgg cgcggggccc gagatgccgc
     3481 cgtctttgcg gatgcgccag aagtgcacag ccataaatat cgacgcgacc agcggaatac
     3541 cgatgcagtg ccagatataa gatcggagca aggcattcga atccacaatg gatccaccca
     3601 acaacccaaa ccgcacgtcg ttgtaagcag tcaaaccgag ctgcggaccg aaaggtccct
     3661 cactgccaag catcggcgtc gcgcgcgcca tgtttgtccc taccgtcacc gcccagaaac
     3721 caagctgatc gtccggcaac aggtaacccg taaacgaaag caacagcgtc aacaccagca
     3781 acacgacccc gacgcaccag ttaaactcgc gcggccgctt gtaagaaccc gtcagcacga
     3841 cgcgaaacat gtgcagccag gttgtgatca ccatcaagtg cgccgcccag cgatgcatgt
     3901 tgcgcaacaa cttgccgaac ggaacgtcgt gttcgagata gagaatgtcg cgaaaagcgt
     3961 cggccttcgt cgggtggtaa tagaacatca acaacacacc ggtgaaggtc agcacgataa
     4021 agaggtagaa cgtgatcccg cccatccccc aggtatacgg atagcgcacg gcgtcacgat
     4081 tgatcttcgc cggatgcagg tgcagaaaga cgtttgagag aatcgcgagc gaacgattac
     4141 gtggatcgga gcgatcgtgc gagacgcgaa agatcgactt ccaaagctgc gtctcctgcg
     4201 ggtctttata ctcctttgct tcttcgatcg cgcggtcttt gagtgcgctc gtgccctctt
     4261 taacgcgcga caccaggctc ttactgccgg cttcttcacc ggtagtcaac tgctttgttt
     4321 ctttcggatc agtatccatc tgtgtaaatc cgtggcccta taccctgata taggcattct
     4381 cgtcttcgaa ttcgttcgtg ccgcccttcg gccattcgta gagggtccca acgtcaacca
     4441 cgatctgccc ttcggcatcc agcgatactt tcgcgcggtc catcggtctt ggcgccggac
     4501 cttcgaacgc gatgccttcg ctgtcgtagc cgctgccgtg acacgggcac ttaaacttgt
     4561 tttcgcttgc cttccagtct ggcgtgcagc cgagatgcgt gcaccgcgca tagatcacga
     4621 acagccggtc ggacgttcgc gtcacccaaa tacgatactg ttgctgccac ttggtatcga
     4681 cgccaagcgc gtaatcgctg ggtggcccaa tgcgaaagac gctggaaggt tcgaggattg
     4741 atcgcggcag aaagaaccgc acgaacatca gaaagaaagt gatcaacgag ccggcgaccg
     4801 tcgcccacac aatgcggcgg cgcattttgt tcacaggagg atcagaagtt gtctcgaccg
     4861 tcgccggttt agccggcgca gcggcgccaa cgttggcttg ggatggcacg tttactgccg
     4921 taccggctgg tcgggcggat atttgatcgg cttgactatc caagtcgaaa cctccgtcac
     4981 ttcattccta attgcttggc gttgcgaact tccgtgggta cactgcgacc ttgctag
//
LOCUS       DASTEY010000314         8911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_880208, whole genome shotgun sequence.
ACCESSION   DASTEY010000314 DASTEY010000000
VERSION     DASTEY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880208"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFO72_05490"
     CDS             <1..519
                     /locus_tag="VFO72_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478774.1"
                     /translation="PDRPENGAPSGFALMAAESHLRHVRGQLPLEVETTDPHEISAWF
                     ANKVNFNVKLPGYQESSGQEKLYKLEGARLVGYQDDYAAYVSYNMKDRPISLVITSEL
                     VARPAGGEQINSRGLTFHFNAIDGLKVITWSDRGLTYALVSDLDERGQQSCIVCHQGT
                     KDQDFIEPLKPH"
     gene            complement(650..2053)
                     /locus_tag="VFO72_05495"
     CDS             complement(650..2053)
                     /locus_tag="VFO72_05495"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA Peptidase M64"
                     /protein_id="HET9478775.1"
                     /translation="MTNRVLVVLLALLALSTSAFAAQTMRVDYYHTGDASQELFSVDR
                     VVIEPLPWPGHASQAIDTSNLGKYLFEVRDAKTDQLLYSRGFASIYGEWETTDEAKST
                     KRTFQESFRFPAPAAPVNIVLRKRDAKNVFKDIWTTTIDPADQFVDRSKQVAPAPLIT
                     IQKAGEPENKVDFLILGDGYTAAEAKKFEADARRLTEVLFATSPFKENRRHFNVWALC
                     PPAQESGVSRPSTGIYRDSPIGATYDAFGSERYVLTFDNRALRRTAQFAPYEFIEVLV
                     NNRTYGGGGIFNLYSTVASDNAFANYVFVHEFGHHFAALADEYYTSSVAYAPATDRVE
                     PWEPNVTALLDVSTLKWRHLVSPFANVTPIPTPWNKEAFEAYSREIQKRRAQLRKDKR
                     PEEEMEALFKQELAHTLKMFAAEKHFGQVGAFEGAMYEARGYYRPEVDCLMFSRTDRF
                     CRVCREAIERVIKMYSI"
     gene            2119..3804
                     /locus_tag="VFO72_05500"
     CDS             2119..3804
                     /locus_tag="VFO72_05500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9478776.1"
                     /translation="MASVLEIIPLGGIGEFGMNCMAVRCGDEMLILDAGMGFPEETAY
                     GVDVSIPNFNFLDEYRDSITAIVLTHGHEDHLGALPYLLKKFNVPVYCSHFTAGLAES
                     KLEEHDLLGDVLIHRVEPRDVVDIGVFSVEFIRVSHSLVDCFSLGIKTPVGTIIHTGD
                     YKVDETPVIGEPIDLRSFRRYGQDGVLALLSDSTNATVPGRTPSERAVIPAFEEIFSE
                     AKGRIVVAAFASSIHRLQIVLDVAQQFNRHVCVLGRSMQKNVEVAERLGYLDIPDGLL
                     VSLNEAKLMSDHEVVFLVTGSQGESRAALSQMATQSYKGLMIEEGDTVVLSARIIPGN
                     ERLISRMIGFIYKRGANIIEEKRRLVHVSGHASQEDIRIMTEAVRPKFVVPVHGEYRM
                     LFRHKEFVKNHLGYAEENIILIENGDVLELDGERAIVVDKREIGRTFIDESGFEEIDS
                     ETVRERKQLAYEGTITVVVTIDEESGELQDEPRLVARGVSGLGTNGFANGSAGNYLLA
                     DAKRVVAASITGASRQTLADETLLKEHVRVELKRFIQKQTGARPVIMPVIVLV"
     gene            3899..4642
                     /locus_tag="VFO72_05505"
     CDS             3899..4642
                     /locus_tag="VFO72_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9478777.1"
                     /translation="MITGASSGIGKGLALEIAARGGRLGLLARREDVLNEIVNQIRSR
                     NGTAIAVAADVRDARAVRAAADRIRAELAPIDVMIANAGIGTTKHASQLNPDEVAEVI
                     NVNVIGAVNSVSAVVPEMVERGSGQLVAISSLAGYRGLAKSAAYCASKAALSSFFESL
                     RIDLHDTGVTVTIIHPGFIKTPLTAGRGAKMPYLMELDDAVKKIVSAIEKGKKSYAFP
                     WQLATIVRASMIMPPAMYDWIAERNSFRE"
     gene            4639..5520
                     /gene="uppP"
                     /locus_tag="VFO72_05510"
     CDS             4639..5520
                     /gene="uppP"
                     /locus_tag="VFO72_05510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HET9478778.1"
                     /translation="MTILQAIVLGLVQGLTEFIPVSSTAHLVFAARVVNLYGGADKTL
                     QAEQTTATIAVIQLGTLLAVLVYFSRDIVNITRAFVGDNLALLRGRGETTGPKLSSDA
                     WLGWLVIIGSVPVGIVGLLFKKQIEGPFTKNLWVIATMMIVVGLLLTIAEMVGKRDHA
                     MDRLGIKDALAVGWAQVLSLIPGSSRSGSTIMGGLFAGQTRETAARFSFLLSIPAIAA
                     SGLLELGEAIEKLPEGSYGALAVATIVSGAVGYASIWFLLRYLRTHSTGVFIVYRVIV
                     GGLILVALSAGYISASI"
     gene            5546..5839
                     /locus_tag="VFO72_05515"
     CDS             5546..5839
                     /locus_tag="VFO72_05515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9478779.1"
                     /translation="MDQRPRSPRVPVEFALEVEGNDQAGKPFHTKATATKISRGGATL
                     IIDVDVPVGSMVTLAPAAGGKLEAEVNGSWIDDIDGTRRIGVKLLNANGWFGE"
     gene            5859..7562
                     /gene="recJ"
                     /locus_tag="VFO72_05520"
     CDS             5859..7562
                     /gene="recJ"
                     /locus_tag="VFO72_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9478780.1"
                     /translation="MKAKRWNVRRQNADAAAALARVLGVSPILAALLINRGYADECAA
                     RAFLSPTYDQLHEPYLMLGMREAVARLKRAIETREPILIYGDYDVDGTTGTAILLRAL
                     KLLGARAGFHIPHRFTEGYGIQQPALERAFAGGYKLVVSVDCGIRAHEPLYWARDNGL
                     DVIITDHHLPDEDEGAPPAFAVLNPNQAGCEYPDKNLAGVGVAFKLVHALFREYGREA
                     QVAAFMKVVAIGTVADVAKLIGENRTIVAIGLKDLARAANPGLKALIDVAGCGDGKGM
                     TAYDLGFRLGPRINAAGRMDAASAVVELFDTRDSAEARRLAEHLDARNEERKTVQQQI
                     IDLALAELGDGPEARVAVIEGSGWHRGVIGIAASKIAERLNRPCVVLSVDGDVAHGSG
                     RSIEAYHLLNGLTACADLFEKFGGHSHAAGITIRPERIAEFRRRLNEHAASCLTAEDL
                     EHSVAIDLELPAEKVTFQLARELEALEPFGAGNPRPVFATRNLRCLSEPVVLKDRHLK
                     LRLAGPQNRPLEAVWWNCIETAGQTPEVNGSIELAYALELNAWNDEYRMQLSVVDLRL
                     E"
     gene            7578..>8911
                     /gene="lptC"
                     /locus_tag="VFO72_05525"
     CDS             7578..>8911
                     /gene="lptC"
                     /locus_tag="VFO72_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET9478781.1"
                     /translation="MQALTRKRSFGIGWRARVPLIARIVAFVLLVSGIAVVAVSYYKL
                     RNVEKFRAKSEKPELSKEVTGVVEGYEQRVTKDGRLYMHVKASRDITFSDNHHELENV
                     SMAVYPVEGDVPDQISAARAIYQPDTNIVSFAGNVKIDTKEKLQVATESLSFDQNSGV
                     AQTDDAVAFNRENVSGTATGAVVEQKAKRLDLKKDVAIKVEPQTNGSNRSGTRARPVT
                     IRAAHGTFEQESLKLTFGGGATVEQERDILSGETLYATLNQEKRLQKAEIRGNAYLRT
                     MEPGRAAEVHAVDMDFFLDQDQRLERAFANNDVKARSLDAESDFELSGSSSLEVLFQA
                     TADTSLLKQMNAGGRSVITLSAPKSKASDPRAANKRLTADSVKLSWRGSGRDLEKAEA
                     SGNAELFIEPVTSSDRAERRKLNAPQFDCDFFETGNLARTCKAAGGAKAVMDP"
BASE COUNT         1912 a         2358 c         2748 g         1893 t
ORIGIN      
        1 ccggatcgtc cagagaatgg agcgccttcg ggatttgctt taatggcggc cgagagtcac
       61 ctgcgtcacg tgcgtggaca gttgccgctg gaagttgaaa cgacggatcc gcacgagatc
      121 tccgcgtggt ttgccaacaa agttaacttc aacgtaaagc tgccgggtta tcaggagtct
      181 tcagggcagg agaaactcta caaactggaa ggcgcgcggc tcgtgggtta tcaggacgac
      241 tacgctgctt acgtttccta taacatgaag gaccgcccga tcagtctggt aatcacctcc
      301 gaactggtcg caaggcctgc cggcggcgag cagatcaact cacgtggttt gacgtttcac
      361 ttcaacgcga tcgatgggtt gaaagtgatc acctggtccg atcgcggatt gacctacgcg
      421 ttggtgtcgg accttgatga acgcgggcaa cagtcgtgca tcgtctgtca ccagggcacc
      481 aaggaccagg atttcatcga gcccctgaag ccgcactaat tccgaagacg tttaccgatt
      541 accgagacgt ttaccctggg accgcaggcg tttcgcctgc acagcttcga acgggcgctc
      601 tgcgccggca tgcaggcgag acgcctgcgg tcccaggata aacaggccat taaatcgagt
      661 acatctttat tactcgttcg attgcttcgc ggcagactcg gcagaaccga tctgtgcgcg
      721 aaaacatgag gcagtccact tccggacggt agtaaccgcg cgcttcgtac atcgctcctt
      781 caaaagcgcc gacctgtccg aaatgcttct cggccgcaaa cattttcagc gtgtgtgcga
      841 gttcttgctt gaacaacgct tccatctctt cttccggtcg cttgtctttg cgcaattgcg
      901 cgcgccgttt ttgtatctcg cgcgaatagg cttcaaacgc ttccttgttc cacggcgtcg
      961 gaataggcgt gacgttagcg aacggtgaga cgaggtgacg ccacttcaac gtgctcacgt
     1021 ccagcagcgc cgtgacgttc ggttcccacg gctcgactcg atctgtcgcc ggcgcatacg
     1081 ccactgatga ggtgtaatac tcatcggcaa gagccgcgaa atgatggcca aactcgtgca
     1141 caaagacgta gttcgcgaat gcgttgtcgg aggctacagt gctgtagaga ttgaagattc
     1201 caccgccgcc gtaggtgcgg ttgttaacca gtacttcgat gaactcgtag ggcgcgaact
     1261 gcgcggtacg ccgcaacgcg cgattgtcaa acgtcaacac atatcgttcg gaaccaaagg
     1321 catcgtacgt cgcgccgatc ggtgaatcgc ggtagatgcc ggtcgatggt ctcgagacac
     1381 cggattcctg agcgggcgga caaagcgccc agacgttgaa atggcgccgg ttctctttga
     1441 acggcgacgt ggcaaacaag acttcagtca gccgtcgtgc gtcggcttca aacttcttgg
     1501 cctctgccgc ggtgtaaccg tctccgagaa tcaggaagtc gactttgttt tccggttcgc
     1561 cggctttctg aatggtgatg agcggcgcgg gcgcgacctg ttttgaacga tcgacaaact
     1621 ggtcggcggg atcgatggtg gtggtccaga tgtctttaaa aacgttcttg gcgtcccgtt
     1681 ttctcaagac gatgttgacg ggcgcagccg gcgccggaaa acgaaacgac tcctgaaacg
     1741 tacgcttggt cgacttcgct tcgtctgtcg tttcccattc gccgtaaatg gaggcgaacc
     1801 cgcgtgagta gagcagctgg tctgtttttg cgtcgcgtac ttcaaataga tacttgccga
     1861 ggttcgacgt gtcgatcgcc tgcgatgcgt gaccgggcca cggcagcggt tcgatcacga
     1921 cgcggtcgac gctgaatagt tcctgcgaag catcgccggt gtggtagtag tcaacccgca
     1981 tggtctgggc tgcgaaggcg ctggtcgaaa gggccaacaa ggcgagcagg acgaccaata
     2041 ctctgtttgt catgactcgg ataatacaga tgaaaatgga tctgtgtcat ccgtggtgat
     2101 ctgtggcact atacgcttat ggctagtgtc cttgagatca ttccgctcgg cgggatcggc
     2161 gagtttggaa tgaactgcat ggcggttcgt tgcggcgacg agatgttgat tctcgacgcc
     2221 ggcatgggct ttcccgagga aacagcgtac ggcgtcgatg tttcaattcc aaatttcaat
     2281 tttctcgacg aataccgcga ctccattacg gccatcgtac tcacgcatgg ccacgaagat
     2341 catctcggtg cgctgccgta cctgctgaag aagttcaacg ttcctgttta ttgctcgcac
     2401 ttcacggccg gcctggccga aagcaaactc gaagaacacg atctgctcgg cgacgtgctg
     2461 attcatcgcg tcgagccgcg cgacgtggtg gacatcggcg tcttcagtgt ggagttcatt
     2521 cgcgtttcgc actcgctggt cgactgcttc tcgctgggca tcaagacgcc tgtcggcacg
     2581 atcattcaca ccggcgatta caaggttgac gaaacacctg tgatcggcga accaatcgac
     2641 ctgcgttcgt ttcggcgtta tggacaggac ggcgtgttgg cgttgctttc cgattcgacc
     2701 aacgcgactg tccccggccg aacgccttcg gaacgcgcgg tgattcccgc tttcgaagag
     2761 atcttttccg aagcaaaggg ccgtattgtt gtggctgcgt ttgcttcgtc gattcatcgt
     2821 ttgcagatcg tcctggacgt ggcgcaacag ttcaaccgtc acgtttgcgt gctcggcaga
     2881 tcgatgcaga aaaatgttga agtggcggaa cggctcgggt atctcgacat tccggacggg
     2941 ctgctggttt cgctcaacga agcgaagttg atgagcgatc acgaggtcgt gtttctggtt
     3001 accggatcgc agggcgagtc gcgcgcggca ctgtcgcaga tggcgacgca gagttacaaa
     3061 ggactgatga tcgaagaagg cgacacggtc gtgctttccg cgcgcatcat tcccggcaac
     3121 gagcgtctga tttcgcggat gatcgggttc atctataagc gtggcgcaaa catcatcgag
     3181 gagaagcggc gtttggtgca cgtcagtgga cacgcatcgc aggaagacat ccggatcatg
     3241 acggaagccg tgcggccgaa gtttgtggtg ccggttcacg gtgagtacag gatgcttttc
     3301 cgtcataagg agtttgtaaa aaatcatctc ggctacgcgg aagagaacat aattttgatc
     3361 gagaatggcg acgtgctcga actcgatggc gagcgtgcga ttgtcgtcga caagcgagag
     3421 attggacgca cgttcattga cgagagcggg tttgaagaga tcgacagcga gactgttcgc
     3481 gagcgcaagc aactggcgta tgagggaacg atcacggtcg tggtgacgat tgatgaagag
     3541 tcgggcgagt tgcaggacga gccacgatta gttgcgcgcg gcgtgagcgg tctaggcacg
     3601 aatgggtttg cgaacggatc ggcggggaat tatctgctgg ccgatgcgaa gcgagtggtg
     3661 gcggcctcaa tcaccggtgc ttcgcggcag acgctggctg acgagacgtt gttgaaagag
     3721 cacgtacggg tagagttgaa gcgcttcatt caaaaacaga ccggtgcacg gccagtgatc
     3781 atgccggtta tcgtgctggt ttagagccac ggacttacac gaatgaacac ggaccgcaga
     3841 aatggtagtc cgtgttcatt cgcgtagctc tgtggctaaa aggtggaaca aaacggtgat
     3901 gattaccggc gcatcgtccg ggatcgggaa gggtctggcc ctggagatcg ctgcgcgcgg
     3961 tggacggctg ggcttgctcg cgcggcgcga ggacgtcctc aatgagatcg tcaatcaaat
     4021 ccggtcgcga aacggaacgg cgatcgcggt cgcggctgac gtgcgcgacg caagagctgt
     4081 tagggcagca gcggatcgaa ttcgtgccga gcttgcacca atcgacgtga tgattgccaa
     4141 tgcgggtatc ggtacaacca aacacgcgtc gcaactgaat ccggatgagg tggcggaagt
     4201 catcaatgta aatgtgatcg gcgcggttaa cagcgtgtca gccgtggtgc cggagatggt
     4261 tgagcgtggg agcggacagt tggtggcgat ttcgagtctc gctggttatc gcggtctggc
     4321 gaaatcggct gcgtactgcg cgagcaaggc ggcgctatcg tcgttttttg aaagtttgag
     4381 aatcgatctg cacgacacgg gcgtgacggt gacgatcatt catcccggtt tcataaagac
     4441 gccgctgacc gccggccgtg gagcgaagat gccgtacctg atggagctcg acgacgcagt
     4501 taaaaagatc gtttcagcaa ttgaaaaagg aaagaagagt tacgccttcc cgtggcaact
     4561 tgcgaccatc gtgcgcgcga gcatgatcat gccgcccgcg atgtatgatt ggatcgcaga
     4621 aagaaactcg tttcgcgaat gaccattctt caagctatcg tgctcggtct ggtgcagggc
     4681 ctgaccgaat tcattccagt cagttcaacc gcacatctcg tctttgccgc acgcgtcgtg
     4741 aatctttacg gcggcgccga caagacgctg caagccgaac agaccaccgc gacgatcgca
     4801 gtgatccagc tcggcacgct gctggccgtg ttggtctatt tctcgcgcga catcgtcaat
     4861 atcacgcgtg cttttgtggg cgacaatctt gcgttgctga gaggtcgtgg tgagacgact
     4921 ggaccaaagc tctcgagcga tgcgtggttg ggttggcttg tgatcatcgg ttcggtgccg
     4981 gttggaattg tcgggctgtt gttcaagaaa cagattgaag ggccatttac gaagaacctt
     5041 tgggtgattg cgacgatgat gatcgttgtg ggcctgttat taacgatcgc ggagatggtc
     5101 ggcaaacggg accatgcgat ggatcggctt ggcatcaagg acgcgcttgc ggttggttgg
     5161 gcgcaggtgc tttcgttgat accgggctcg agccgttccg gttcgacgat tatgggcggg
     5221 ttgtttgcgg gccagacgcg tgaaaccgca gcgaggtttt cgtttttgtt gtctattccg
     5281 gcgattgctg cgagtgggtt gttggaactg ggagaggcga ttgagaaatt gcctgaggga
     5341 agctacggcg cgctggccgt agcgacaatc gtttccggcg cggttggtta cgcgtcgatc
     5401 tggtttctgt tgcgttattt gcgcacgcat tcgacgggag tgtttattgt gtatcgtgtg
     5461 attgtggggg gattgattct ggtagcctta tcagcggggt atatttcggc aagtatttag
     5521 gccacggatt agagggattt tcaaaatgga tcagagacca cgcagcccgc gagttccggt
     5581 cgagtttgct ctggaagttg aaggcaacga ccaggcgggg aaaccatttc acacgaaagc
     5641 gacggcgacg aagatcagtc gcggcggcgc gacgctcatc atcgacgtcg atgtgccggt
     5701 gggatcgatg gtgacactgg cgccggccgc tggtggtaaa ctggaggccg aagttaacgg
     5761 ttcgtggatt gacgacatcg acggcacccg ccgcatcggt gttaagctgc tcaacgcgaa
     5821 cggctggttc ggcgagtgat ggatcgcatg acaactaaat gaaagccaaa cgctggaacg
     5881 tgcgacggca gaacgcggac gccgccgccg cgctcgcacg cgttttgggt gtctcaccaa
     5941 tcctggctgc tctactcatc aatcgcggtt acgccgacga atgcgccgcg cgcgcgttcc
     6001 tttcgccaac ttacgatcaa cttcacgaac catacctcat gctcggcatg cgcgaggccg
     6061 tcgcgcgtct caaacgcgcg atcgaaacgc gtgaaccgat actgatctac ggcgactacg
     6121 acgtcgacgg taccaccggc accgccattc tgctccgcgc actgaaactt ctcggcgcgc
     6181 gcgccggttt tcatatcccg caccgcttca ccgaaggcta cggcattcaa caacccgccc
     6241 tcgaacgcgc cttcgccggc ggttacaaac tcgtcgttag cgtcgactgc ggcatccgtg
     6301 cccacgaacc actgtactgg gcgcgcgata acggtctcga cgtcatcatc accgatcacc
     6361 atctgcccga cgaagacgaa ggcgcgccac ccgcgtttgc cgtgctcaac ccaaaccagg
     6421 ccggttgtga atacccggac aaaaacctcg ccggtgtcgg cgtcgcgttc aaactggttc
     6481 acgcactgtt tcgggaatat ggccgcgaag cgcaagtggc ggcgttcatg aaggttgtcg
     6541 ccatcgggac cgtcgctgac gtcgcaaaac tgataggcga gaaccgcacg atcgtggcca
     6601 ttggactcaa ggatctggct cgtgccgcca atccgggact caaggcgtta atcgatgtcg
     6661 ccggttgcgg agacggcaag ggcatgacag catacgatct gggttttcgg ctcggaccac
     6721 gaattaacgc ggccggacgt atggacgcgg cgagtgccgt cgtcgagctt tttgacacac
     6781 gcgactccgc ggaagcgcga cggctcgcgg aacacctcga cgcgcgaaac gaggagcgca
     6841 agactgtcca gcaacagatc atcgatctgg cactcgcgga gctgggcgac gggcctgaag
     6901 cgagggttgc ggtaatcgag ggttcgggtt ggcatcgcgg cgtgatcggt attgcagcgt
     6961 cgaagatcgc cgagcgactg aaccgaccgt gtgtcgtgtt atcggttgat ggtgacgtgg
     7021 cgcacggctc gggccggagc atcgaggcgt accatctgtt aaatggtttg acggcttgcg
     7081 cggatctgtt tgaaaagttt ggcggacatt cgcacgcggc agggatcacg attcgcccgg
     7141 agcggattgc tgagtttcgc cggcgtttga atgaacatgc cgcgtcgtgt ttgacagcgg
     7201 aagatctcga acattcggtc gcgattgatc tggaattgcc ggcggagaag gtgacgtttc
     7261 aactcgcgcg cgagctggaa gcgctggagc cgtttggcgc gggcaatccg cgcccggtgt
     7321 ttgcgaccag gaatctgcgt tgtttgtcgg agccggtagt gctgaaagac cggcatttga
     7381 aattgcgact ggcaggtccg cagaatcgcc cattggaggc ggtttggtgg aactgtatcg
     7441 agaccgcggg gcaaacgcct gaagtaaacg gcagcatcga actcgcctac gcgctcgaac
     7501 tcaatgcgtg gaacgacgag tatcgcatgc agctaagcgt tgttgatttg cgactagagt
     7561 agacacggat tttacggatg caagcgctga cacgaaaacg ttcatttgga atcgggtggc
     7621 gagcgagagt gccgctgatc gcgcgcattg tggcgttcgt gctgctcgtg tcaggcatcg
     7681 ctgtcgttgc tgtctcttat tacaagttgc gcaacgtcga gaagttcagg gcgaaatctg
     7741 aaaagccgga actctcaaag gaagtcaccg gcgtcgtcga agggtatgaa cagcgcgtaa
     7801 ccaaagacgg ccggctctac atgcacgtga aggcctcgcg cgacatcacg ttttctgaca
     7861 accatcacga actcgaaaac gtcagcatgg cagtctatcc ggtcgaaggc gacgttcccg
     7921 accaaatctc agccgcacgc gcaatctatc aacccgacac caacatcgtc tcattcgccg
     7981 gcaacgtaaa gatcgataca aaagagaaac ttcaagtcgc cacggaatcg ctctcttttg
     8041 atcagaacag tggcgtggcc caaaccgacg acgccgtcgc attcaaccgc gaaaacgtgt
     8101 ccggcactgc aaccggcgca gtcgtcgaac agaaagccaa gcggctggac ctgaaaaagg
     8161 acgtggcgat aaaagtcgaa ccgcagacta acggatccaa cagatcggga actcgagcgc
     8221 ggcctgtcac gattcgcgct gcgcacggca cattcgaaca agaatcactc aagcttacct
     8281 ttgggggcgg ggccacagtc gaacaggagc gcgacatcct cagcggcgaa actctctacg
     8341 ccacactcaa tcaggaaaag cgtctccaga aagcggagat ccgcggcaac gcgtacctgc
     8401 gcacgatgga accgggtcgc gccgccgaag tccatgccgt cgatatggac ttttttctcg
     8461 accaggacca gcgattggaa cgcgccttcg ccaacaacga cgtcaaggcc cgttcgcttg
     8521 atgcggagtc ggatttcgaa ctcagcggca gcagttcgct cgaagttctc ttccaggcca
     8581 ctgcggacac gagtctcctg aagcagatga atgcgggcgg gcgttcggtg atcacgttga
     8641 gtgcgccgaa gtcgaaggca agcgatccgc gtgcggcaaa taaacgattg acggctgatt
     8701 cggtgaagtt gagttggcgc ggttccggac gcgatctcga aaaagcagaa gcttcaggaa
     8761 acgccgagtt gttcattgaa ccggtcacga gcagcgaccg cgcggagcgg cggaaactga
     8821 acgcgccgca gttcgattgt gatttttttg aaaccggcaa tctcgcgcgg acctgcaaag
     8881 ctgccggcgg tgcgaaagcc gtgatggatc c
//
LOCUS       DASTEY010000315         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_352583, whole genome shotgun sequence.
ACCESSION   DASTEY010000315 DASTEY010000000
VERSION     DASTEY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352583"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFO72_05530"
     CDS             <1..650
                     /locus_tag="VFO72_05530"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9478782.1"
                     /translation="KRGHWHEEEIAAALASLSGWIDQVPPMYSAKKREGKKLYELARR
                     GEEVDREPVRVCIHEFTAIKSHGQLIKDNLDGTYDFRVHVVCSSGTYVRVLAADFGKR
                     LSVGAHLAELRRTRVGDFRVENAVTLDDLKMSFAEEALGALVVPPGTALSKLPFVHLS
                     DQDVRNVSHGREVKVEGSGWTDGENVKMCDGQEQLIAVGQYDARAQLLHPRVVLR"
     gene            941..>2134
                     /locus_tag="VFO72_05535"
     CDS             941..>2134
                     /locus_tag="VFO72_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478783.1"
                     /translation="MQEQDGNKGFNISERLEDLRREHEALRWEGTFRDYFELVAQNPR
                     IAQLSHARINDMVHAAGVERLNEGTQHEVLSYNFFASELFGIEEPIARIVEYFKSAAQ
                     RLEVRKRILLLMGPVGGGKSTIVNMLKRGLEDWTRTDGGAVYAIKDCPMHEEPLHLIP
                     HQLRSEVEKHYGIYIEGDLCPQCRYSLDKIYGGRHEDVKVHRVVFSEKERVGIGTFSP
                     SDPKSQDITELTGSIDLSTIGEVGVESDPRAYRFDGELNIANRGLMEFVEMLKVDEKF
                     LYSLLTLSQEQSIKTGRFAMIYADEVILSHSNENEYIAFAGNRKSEALQDRIILVKVP
                     YNLRVSQEERIYDKLLHQSEALRNVHLAPNTLKVAAMFAVMTRLEEPKRQNVDIVKKM
                     KLYDGE"
BASE COUNT          505 a          565 c          610 g          454 t
ORIGIN      
        1 agaagcgcgg acattggcac gaagaggaga tcgcagcagc gctcgcgagt ctgagtggct
       61 ggatagatca ggtgccaccg atgtactcag ccaaaaagcg cgaggggaaa aagctctacg
      121 aacttgcgcg acgtggcgaa gaggtggacc gtgagccggt ccgtgtttgc attcatgagt
      181 tcactgctat caagtcgcac ggccaactga tcaaagacaa tctggacggc acgtacgatt
      241 tccgcgttca tgtcgtttgc tcttcgggca cgtatgtgcg ggtgctagcc gcagattttg
      301 gaaagcgtct gtccgtcggc gcacatttgg ctgagttacg tcgcacgcga gtcggagact
      361 tcagggttga gaacgcggtc acgctggacg acttgaaaat gagttttgct gaggaagcgc
      421 ttggtgcgtt ggtggttccg cccggcacag cgctctcgaa actccctttc gtgcatctaa
      481 gcgaccagga tgtgcgtaat gttagtcatg gaagagaggt aaaagttgaa ggatcgggtt
      541 ggacggatgg cgagaacgta aagatgtgcg acgggcagga gcagttgata gctgttggcc
      601 agtatgacgc gcgtgcgcaa ctgttgcatc cgcgcgtggt gcttcgctga aaaagcattg
      661 acttaggggg tttatgcgtg tggtatactg caatcccaag gaagcgctca ggtgctcgct
      721 tccatcaagg tgcttccctc cgctagttcc gttgacacgc tcattgagaa ctgatgcttc
      781 gaggacgtag ctcacgctta cgcgcgcgtg aggcgtatca cgaggtagtt aattttattt
      841 gacccaaccg cattccccga tcgctggcgg ttgtttttac gaagcgtggc ctctccccac
      901 acgctgtcca acgcgcgaaa gccgaagagg tttcaaggct atgcaagagc aggatggcaa
      961 caaaggattc aatatcagtg aacgcttgga agatctgcgc cgcgaacacg aagcgctgcg
     1021 ctgggaagga acatttcgcg actacttcga gctggtagcc cagaaccccc gtatcgcgca
     1081 actctcgcac gcgcgcatca acgacatggt tcatgccgcc ggcgtcgagc ggctcaatga
     1141 aggcacccag cacgaagtcc tttcctacaa tttctttgcc tccgagctct ttggcatcga
     1201 agaacccatc gccagaattg tcgagtactt caaatcagcc gcgcagcgcc tcgaagtgcg
     1261 caaacgcatc ctgctgttga tgggtccggt cggcggtggc aagtcaacca tcgtcaacat
     1321 gctcaaacgc ggactggaag actggaccag aaccgacggc ggcgcggtct acgcgatcaa
     1381 agactgcccg atgcacgaag aaccgctgca tctcattccg caccaactgc gctctgaagt
     1441 cgaaaaacat tacggcatct acatcgaagg cgacctctgt ccacagtgcc gttattcact
     1501 cgataaaatt tacggcggac gccatgaaga cgtgaaagtc catcgcgttg tcttctctga
     1561 aaaagaacgc gtcggtatcg gcacgttttc gccgagcgat cccaagtcgc aggacatcac
     1621 tgagctgacc ggctcaatcg atctttcaac cattggcgag gtcggcgtcg aatcagatcc
     1681 gcgcgcctat cgtttcgacg gcgagttgaa tattgccaat cgcggtttga tggaattcgt
     1741 cgagatgctg aaggttgatg agaagtttct ctacagcctg ctgacgctgt cgcaggagca
     1801 gagcatcaag accggccggt tcgcgatgat ttacgccgac gaagttatcc tttcacactc
     1861 gaacgagaac gagtacatcg ccttcgccgg caatcgcaaa tccgaggcgc ttcaggaccg
     1921 catcattttg gtcaaggtcc cgtacaacct gcgcgtttcg caggaagaga gaatttacga
     1981 caagctgttg catcagtcag aagctctgcg caacgttcac ctggcgccga acacgctgaa
     2041 agtcgcggcg atgtttgccg tgatgacgcg cctcgaagaa ccaaagcggc aaaacgtcga
     2101 catcgtcaag aagatgaagc tttacgatgg tgaa
//
LOCUS       DASTEY010000316         5425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_72637, whole genome shotgun sequence.
ACCESSION   DASTEY010000316 DASTEY010000000
VERSION     DASTEY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72637"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /gene="rplD"
                     /locus_tag="VFO72_05540"
     CDS             <1..683
                     /gene="rplD"
                     /locus_tag="VFO72_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET9478784.1"
                     /translation="PNRRVMPTVKVRNLQNEEVGEVELSDVVFGAPLNQGLIHAAVRN
                     FMANARQGTSATKTRGDVSGAGRKLWKQKGTGRARIASLRSPLWKGGGNVHGPQPRDW
                     SYNMPKKMRQGALRSALSERVREGNVFVVEGWDLDKPKTKDFSQSLGKLGLAGKTLIV
                     DSLDNENLMLSARNLRHTKVVNSFGLNIYDLLYHDQLVLSREAATELEQLLGNAKGET
                     SPETQEAA"
     gene            687..992
                     /locus_tag="VFO72_05545"
     CDS             687..992
                     /locus_tag="VFO72_05545"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET9478785.1"
                     /translation="MRTVWDIIKSPVITEKALAAKEETQDTRQLLTFRVDRRATKPEI
                     KSAVETIFQVKVDHIRTINFMGKMARRGRFEGRKPSWKKAYVTLKQGEKPVDYGEAI"
     gene            1005..1835
                     /gene="rplB"
                     /locus_tag="VFO72_05550"
     CDS             1005..1835
                     /gene="rplB"
                     /locus_tag="VFO72_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9478786.1"
                     /translation="MGIKKLTPTSAARRYQTYLTRDELTLGAVPEKSLLEPKKRISGR
                     NNDGRITVRRRGGGHKRFYRIVDFKRDKEGVPGRVTQIEYDPNRSAHIALITYLDGEK
                     RYIIAPTGLEVGKTIMSGADADILPGNTLPLINIPLGTQIHNIELRPGKGGQMARSAG
                     TFAQLVAKEGEYAQLRMPSGEVRRVPLVCRATVGQVGNIEHENVSLGKAGRSRWKGLR
                     PKVRGVAMNPVDHPHGGGEGKTSGGRNPVTPWGQPTRGYKTRSNKRTQKMILKDRRTK
                     "
     gene            1876..2181
                     /gene="rpsS"
                     /locus_tag="VFO72_05555"
     CDS             1876..2181
                     /gene="rpsS"
                     /locus_tag="VFO72_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET9478787.1"
                     /translation="MARSVKKGPYIDKGIMKAVEKARAGGQRPPVIKTWSRRSTITPD
                     MVGLTFGVHNGKRHLPIYVTENMVGHKLGEFAPTRTFKGHSGTKVEKASKVAAAPAK"
     gene            2193..2534
                     /gene="rplV"
                     /locus_tag="VFO72_05560"
                     /pseudo
     CDS             2193..2534
                     /gene="rplV"
                     /locus_tag="VFO72_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            2771..3535
                     /gene="rpsC"
                     /locus_tag="VFO72_05565"
     CDS             2771..3535
                     /gene="rpsC"
                     /locus_tag="VFO72_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET9478788.1"
                     /translation="MGQKVHPYGFRLGYNKDWHSHWFAKRQFGEFLLEDLKLKRDLKQ
                     RFAGAGVSHVDIERAANRLKIIIYTSRPGIIIGRKGAEIDKLKSDLSSKTGREVLVSI
                     QEIHKPELNAQLQAEKIAAQLEKRIAFRRAMRKAVEESLRFGAQGIKVKVSGRLNGAE
                     IARSEWHLQGQLPLHTLRADIDYGLAEAQTTFGQIGVKVWIYRGEILDPKAAMARGTG
                     SDPINETRTGERERRPRTRREREGGSDGRRPRTENQ"
     gene            3626..4054
                     /gene="rplP"
                     /locus_tag="VFO72_05570"
     CDS             3626..4054
                     /gene="rplP"
                     /locus_tag="VFO72_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9478789.1"
                     /translation="MLMPKKVKHRKQMRGRMTGVATRGSEISFGEYGLKSLEPAWITD
                     RQIEAARIAMTRHIKRGGKIWIRMFPDKPVTKKPAETRMGKGKGAPEYWVAVVKPGRI
                     LYEIEGVDLATARDAMRLAAQKLPIKTKFVTRADQEGGAI"
     gene            4057..4254
                     /gene="rpmC"
                     /locus_tag="VFO72_05575"
     CDS             4057..4254
                     /gene="rpmC"
                     /locus_tag="VFO72_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9478790.1"
                     /translation="MKRQEELDKYREMAADELRAEAARLRESLFRLNFKLALGEVDAI
                     KRIRQEKKALARIETISGQRS"
     gene            4309..4632
                     /gene="rpsQ"
                     /locus_tag="VFO72_05580"
     CDS             4309..4632
                     /gene="rpsQ"
                     /locus_tag="VFO72_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9478791.1"
                     /translation="MAENQENINESASAPEAAAAATSQSRANRSQKVGTVTSDKMQKT
                     VVVRVDRLVRHRKYRRYVRRTSKFMAHDEMGATVGDKVRIVETRPLSARKRWRVIEIV
                     QKAAK"
     gene            4663..5031
                     /gene="rplN"
                     /locus_tag="VFO72_05585"
     CDS             4663..5031
                     /gene="rplN"
                     /locus_tag="VFO72_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9478792.1"
                     /translation="MIQMQTSLEVADNSGARRVEMIMPIGGSTGKIASLGDRIKVTVK
                     EASPDGTIKKGTVQDAVIVRTRKEVRRADGTYVRFDQNAAVLIKEDGTPIGTRVFGPV
                     ARELRDKNFMKIVSLAPEVI"
     gene            5036..5371
                     /gene="rplX"
                     /locus_tag="VFO72_05590"
     CDS             5036..5371
                     /gene="rplX"
                     /locus_tag="VFO72_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9478793.1"
                     /translation="MAGTKRSKIKKNDQVAIIAGRDKGRRGRVLEVLPGKGKIKVEGA
                     GMIKRHQKANPQANRGGGIVDKESFIDISNVQLIDPQSGKPSRVRYQRESDGSKTRVA
                     ASSGHSVEK"
BASE COUNT         1339 a         1368 c         1767 g          951 t
ORIGIN      
        1 gaccaaacag gagagttatg ccgacagtca aggtgcgcaa tttacagaac gaagaggttg
       61 gcgaggtcga gctgtctgac gttgtttttg gcgcgcctct aaaccagggt ttgatacacg
      121 cggcggtgcg aaatttcatg gccaacgcac gtcagggaac ttccgcgacg aagactcgcg
      181 gcgacgtttc cggcgcggga cgaaagctct ggaaacagaa gggcactggc cgtgctcgca
      241 tcgcgtcgct gcgctcgcca ctctggaaag gcggcggcaa cgtgcatgga ccacagccac
      301 gcgactggtc ctacaacatg ccgaagaaga tgcgtcaggg cgcgttgcgc tccgcgcttt
      361 cggagcgcgt gcgtgaaggg aatgtgttcg tggttgaggg ttgggatctc gacaagccga
      421 agacgaagga tttttcgcag tcgttgggca agctcggttt ggcggggaag acgctgatcg
      481 tcgattcgct tgataacgaa aacctgatgc tgtcggcacg taacctgcgc cacacgaaag
      541 tggtgaacag cttcggtttg aatatctacg atctgctcta tcacgaccag ttggtgcttt
      601 cacgtgaagc ggcaacggag ctcgagcagt tgttaggtaa tgccaagggc gagacatcgc
      661 cggaaacgca ggaggcggcg taagcgatga gaacagtttg ggacatcatc aagtcgccgg
      721 tgattacgga aaaggcgctg gccgcaaagg aagagacgca ggacacgcgg cagttgctga
      781 ctttccgtgt cgatcgacgc gccaccaagc cggagatcaa atcggcggtc gagaccattt
      841 tccaggtgaa ggtggaccac atccgcacga tcaatttcat gggcaagatg gcacggcggg
      901 gacggttcga aggtcgtaag ccttcctgga agaaggccta cgtaactctc aagcaaggcg
      961 agaagccggt tgattacggc gaagcaatat agcggaataa cgagatgggc ataaagaaac
     1021 ttacgccgac atcggcggcg cgacgttatc aaacgtatct gacgcgtgac gagttgacgc
     1081 ttggggcggt tcccgaaaag tctctgctcg agcctaagaa gcggatttcc ggacgcaaca
     1141 acgacggccg cattaccgtg cggcgtcgtg gtggcggtca caagcgcttt tatcgaattg
     1201 tcgacttcaa gcgcgacaag gaaggcgtgc ccggacgcgt tacgcagatc gagtacgatc
     1261 cgaatcgttc ggctcacatc gcgctgatta cttatctcga cggcgagaag cgttacatca
     1321 tcgcgccgac gggtttggaa gttggcaaga cgatcatgag cggtgccgac gcggacattc
     1381 ttccgggcaa tacgctgccg ctaatcaata tcccgctggg cacgcagata cacaacatcg
     1441 agctgcgacc cggcaagggc gggcagatgg cgcgttcggc aggaacgttc gcgcagctgg
     1501 tggcgaagga aggcgagtac gcacagttgc gcatgccgtc gggtgaagtg cggcgagtgc
     1561 ctttagtgtg cagggcgact gtgggccagg tcggcaacat cgagcacgag aacgtttcgc
     1621 ttggtaaggc aggccgatcg cgctggaagg gattgcgtcc caaagttcgc ggtgtggcga
     1681 tgaacccggt ggaccacccg cacggcggcg gcgaaggcaa gacctcgggc ggccgaaacc
     1741 cggtgacacc ctggggccag ccgacacgcg gctataagac gcgcagcaac aagcgcacgc
     1801 agaagatgat tctgaaggat cgcagaacga agtaataggt catatcggtc ctataggacc
     1861 tattgaagga gagagatggc tcgttcagtt aagaaaggcc cgtacatcga caagggcatc
     1921 atgaaagcgg ttgagaaggc ccgcgcgggc gggcagcggc cgccggtgat caaaacgtgg
     1981 tcgcgccgtt cgacgattac gcccgacatg gtcgggctca cgttcggcgt tcacaacgga
     2041 aagcgtcacc ttccgatcta cgtgacggag aacatggtgg gccacaagct cggtgagttt
     2101 gcgccgacgc gcacgttcaa gggccactcg ggaactaaag tcgagaaggc aagcaaggtc
     2161 gcggcagcac cggctaagta aaggattcaa cgatggaagc aacagcgagt gctagatatt
     2221 tgcgaggttc ggcgcagaag gcccggctgg tcgtggatat gattcgcggc aaagacgtta
     2281 accaggcgct ggcgattctt cgtttttcga ataagcgcgc cgccggccat gtcgagaagt
     2341 gcatgcgttc ggctatcgcc aacgccaacg agattgctga gaaagcaaac atctcgatcg
     2401 acccggacga tctctgggta cggacggcgt ttgtcgatcg tgggccaacg aagaaccggc
     2461 gtcgtgtaag acccgcgcca cagggccgtg cctatcgaga acaacgacac ttctgtcacg
     2521 tgacgatctc tctgagtaat gacgagcgtc cggcagcgaa gggaaccagg ccggaagaga
     2581 cggcggaagc aaagccggcg aagctggcgg agagcaaacg ccgcaagcgt ggcggcgctg
     2641 ctgcagcacc gcgagcgaag cgcgcggtga agaaggaaga gccggaagca actaaggaaa
     2701 agccggccac taagaaggct tcgaagaaga aagccaagac cgaagagtaa agacagcact
     2761 ggagaagatt gtgggtcaaa aagttcatcc atacggtttt cgactcggct acaacaaaga
     2821 ctggcattcg cactggttcg ccaaacgaca gttcggtgag ttcctgctcg aagatctcaa
     2881 gctgaagcgt gatctcaagc agcgttttgc cggtgctggc gtgtcgcacg tcgatatcga
     2941 acgagccgcg aatcgcctca agatcatcat ctacacgtcg cgtcccggca tcatcatcgg
     3001 acgcaaaggc gctgagatcg acaaactcaa gagcgatctc tcgtcgaaga cgggccggga
     3061 agtgctggtt tccatccagg agatccacaa gccggaactg aacgctcagc tccaggcgga
     3121 gaagatcgcc gcacagctcg agaagcgcat cgcgtttcgt cgtgcgatga gaaaagcggt
     3181 ggaagagtcg ctgcggtttg gcgcacaggg aatcaaggtc aaggtcagtg gtcgtttgaa
     3241 cggcgctgaa atcgcccgca gtgagtggca cttgcaggga cagcttccgc tgcacacgct
     3301 gcgcgcggac atcgattacg gtcttgccga agcacagacg acgtttggcc agattggtgt
     3361 gaaggtttgg atttatcgtg gcgagattct cgatccgaag gctgcgatgg ctcgcggcac
     3421 cggttcggat ccgatcaacg agacgcgcac cggcgagcgt gagcgccgcc cgcgcacgcg
     3481 acgcgaacgc gaaggtggat cggacggacg aagaccacga actgagaacc aataggagcc
     3541 acaaaaggca caaaagacac aaaatatctg aaccgatttt tatgtgattt tgtgcttctt
     3601 tggggcagga aatgggtgaa acgctatgtt gatgccgaag aaagttaagc accggaagca
     3661 gatgcgcgga cgcatgactg gcgtcgcgac tcgtggcagc gagatcagct tcggtgagta
     3721 tgggctcaag agtctggagc cggcgtggat taccgaccgt cagatcgagg ctgctcgtat
     3781 cgcgatgacg cgccacatca agcgcggcgg caagatctgg attcgcatgt ttccggacaa
     3841 gccggtgacg aagaagcccg cggaaacgcg catgggaaaa ggcaaaggcg cgcctgagta
     3901 ttgggtggcc gtcgttaagc cgggccggat cctttacgag attgagggtg tcgatctggc
     3961 caccgcacgc gacgcgatgc gtctggcagc ccagaagctt ccgatcaaga cgaagtttgt
     4021 gacccgcgcc gatcaggaag gaggcgcgat ctaacaatga agagacaaga agagctcgac
     4081 aagtatcggg agatggctgc ggacgaattg cgtgcggaag cagcgcgcct gcgtgaatcg
     4141 cttttccggt tgaactttaa gctggcgctc ggcgaggttg acgcgatcaa gcgcattcgc
     4201 caggagaaga aagcgctggc gcggatcgaa acgatctcgg gccagcgaag ctaataggtc
     4261 atattaagac ctataggtcc tatatcggcc tattaggagt tgaagagaat ggcagagaat
     4321 caagagaata ttaacgaaag cgcaagcgct cccgaggcgg cggcagcagc gacctcgcag
     4381 agccgtgcga accggtcgca gaaagtcgga acggtaacgt ccgacaagat gcagaagacc
     4441 gtcgtggttc gggtggaccg tctggttcgt caccgcaagt atcgtcgcta cgtgcggcgc
     4501 acgtcgaagt tcatggccca cgacgagatg ggcgcgactg tcggcgacaa ggtgcgcatc
     4561 gttgaaacgc gtccactttc agcgcgcaag cgttggcgtg taatagagat cgttcagaaa
     4621 gctgcgaagt aggcttcgcc gcgagagccg gaggtaacga cagtgattca gatgcaaacc
     4681 tcgctcgagg ttgctgacaa ttcgggggcg cgtcgcgtgg agatgattat gccgattggc
     4741 ggctccaccg gaaagattgc cagcctggga gatcgaatca aggtaacggt caaagaggcc
     4801 tctcccgacg gaactatcaa aaagggcacc gttcaggatg cggtgattgt gcgcacgcgc
     4861 aaggaagtgc gccgcgccga tggaacgtat gtgaggttcg atcagaatgc cgcggtgctg
     4921 atcaaggagg atggcactcc gatcggcacg cgcgtgtttg gaccagtagc gcgcgagctg
     4981 cgcgacaaga atttcatgaa gattgtttcg ctcgcgccgg aggtaattta acgaaatggc
     5041 aggaacaaag cgctcaaaga tcaaaaagaa cgatcaggtg gcgattattg ccgggcgcga
     5101 caagggccgg cgtggacgcg tgttggaagt tttacccggc aagggaaaga tcaaggtcga
     5161 aggcgcgggc atgatcaagc gccaccagaa agcgaatccg caggcgaatc gcgggggcgg
     5221 tattgtggat aaggagtcgt ttatcgacat ctcgaacgtg cagttgattg atccgcaatc
     5281 aggcaaaccc tcgcgcgtgc ggtatcaaag agagagcgat ggttcgaaga cgcgtgtcgc
     5341 ggcttccagt ggacactcgg tggagaagta gacatggctg caaggttaaa agagcgatat
     5401 caaaaggaca tcgcgccgca gattg
//
LOCUS       DASTEY010000317         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1407569, whole genome shotgun sequence.
ACCESSION   DASTEY010000317 DASTEY010000000
VERSION     DASTEY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407569"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFO72_05595"
     CDS             complement(<1..1012)
                     /locus_tag="VFO72_05595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9478794.1"
                     /translation="MTSDYDIIIAGAGPAGSSAAIHLATGGLNVLLVEQKMFPRPKLC
                     GEFISPECRDHFEKLGVARAIACFDPASISETVFYSSRGYKVAVPSRWFGERMALGLS
                     RAVMDDVLLRRAAEVGVKIIEGASVVGPRIYTDCVQGLRIKSASEEREYQAPVTIDAT
                     GRARILLRRLNHLRQQKPKLIAFKAHFQQTRVASGTCEIYFYKGGYGGLSSIEAGTSN
                     LCFLISAEQVKRCQSDPERVMREVVMENPRARYTLDPAQTASEWLSASWEDFGSHGPA
                     PAMGLLAIGDAAAFIDPFTGSGMLMAFESGELAAEVILRQHHQFDAATLAADYTTAYK
                     RKF"
     gene            complement(1009..1710)
                     /locus_tag="VFO72_05600"
     CDS             complement(1009..1710)
                     /locus_tag="VFO72_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9478795.1"
                     /translation="MFDKFKRRSSELEYIDTGNYTPEEYEHCISELQLVNRWMGDAHA
                     LKHTLLREVEEKGLREFTVLDVGAGSGELLRVAATWARATRRGFKGVGLELNERSAQA
                     ILEESKDFPAITSVRGDALRLPFMESQFDYVMCSLFTHHFVDEQVVHILREMNRVARR
                     RIFVIDLHRDPVPYFFYTTLGKLLLKNRLLRHDGALSILRSFKAEELVRLGQAAGLRD
                     LWVEHHFPARLVLSA"
     gene            complement(1773..3056)
                     /locus_tag="VFO72_05605"
     CDS             complement(1773..3056)
                     /locus_tag="VFO72_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET9478796.1"
                     /translation="MLRGFTRPRRVVITGMGCVTPLGIGREAFWDALVAGKSGVRRIE
                     SFDPSSFPVQIAAEVPSFDWEAQLNPKDRKHVPRTVPLALAAAREALADARLFPEDMS
                     LDERRAFGVELGTGGGGLAFTEQQYEYWYVGPTHKASVYTIPASTHGGLSSELSMAFG
                     LRGLSHIVSTGCTSSTDAIAYATQHIALGRQDLMLAGGVDAPIAPGILAAFNLMTVLT
                     NDWNNEPQRASRPFSRNRSGMVLGEGSWIYVLEELKHATSRGAQIYAEITGYGATCDA
                     YHRVRLAEDGDEPARAMRLALHDAGLEAKDIDYVNLHGTSTVLNDRIETSALKLAFDG
                     HAARIPMSATKSQIGHPQGASGAAGLGAALCAMHTNKIPPTINLEEPDPDCDLDYVPN
                     EAREANVETALCNCIGFGSKNSALVVQRINSGSGQ"
     gene            complement(3075..>3719)
                     /locus_tag="VFO72_05610"
     CDS             complement(3075..>3719)
                     /locus_tag="VFO72_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9478797.1"
                     /translation="IAGTNAHFVTEDVEGQLKRTLVSSFSDALGESKIAALDLASNYD
                     ELGKFMRGKMNEDFKAWGLELTKFVIENISLPQEVESAMDKRTSMGVIGDVGRYTQFQ
                     AADAMRDAAQNPSGGAGTGAGLGAGFAVGNAMASAMTDSIRQSKEGGGGGTATAAGGG
                     AAAGVTCPQCGNNNSPGAKFCVECGTKLGAATCPNCQAQLPPGAKFCNECGQKV"
BASE COUNT          772 a         1095 c         1034 g          818 t
ORIGIN      
        1 gaaacttcct cttataagct gttgtgtagt cggccgcgag agtagctgcg tcaaactggt
       61 gatgttgacg aagaatcacc tctgctgcca gttcgccact ttcaaaagcc atcaacatgc
      121 cactgccagt gaaaggatcg ataaacgccg ccgcgtcgcc tatcgccagc aagcccatgg
      181 ccggcgcggg cccgtgactg ccaaagtctt cccacgatgc gctgagccat tcggacgcgg
      241 tttgcgcggg atcgagcgtg taacgcgcgc gcgggttttc catcaccacc tcgcgcatga
      301 cgcgttcagg atcagattgg caacgcttca cctgctcggc actgatgagg aaacaaagat
      361 tactcgtgcc tgcttcaata ctgctcagtc cgccgtaacc gcccttgtaa aagtagattt
      421 cacaagtccc ggaagcgacc cgtgtctgtt gaaagtgcgc tttgaaggcg atcagttttg
      481 gtttttgttg gcgcaggtga tttaacctgc gtaggagaat tcgcgcccgg ccggtagcat
      541 cgatcgtcac cggagcttga tactcccgtt cttcacttgc ggatttaatc ctcagtccct
      601 gtacgcagtc tgtgtaaatc cgtggcccaa ctacactcgc gccttcaatg attttgaccc
      661 caacttccgc tgcgcgtcgc aacaacacgt cgtccatcac ggcgcgactt agtccaagcg
      721 ccattcgctc gccaaaccaa cggctcggaa ccgcgacctt atagccacgg ctagaataga
      781 aaaccgtttc agagatcgat gccggatcaa aacaggcgat cgctcgcgcg acgcccagtt
      841 tttcaaaatg atcccggcat tcgggagaga tgaattcacc gcagagtttt ggccgtggga
      901 acattttctg ttcgacgagc aggacgttga gaccgcccgt ggcgaggtgg atcgcagcgc
      961 tgctacccgc tggaccagca cccgcaatta tgatgtcgta gtcactcgtc atgcactcaa
     1021 taccaaccgt gcgggaaaat gatgctcgac ccaaagatcc ctcaggcccg cggcttggcc
     1081 caggcggacc agctcttcag ctttgaaact ccgcaatatt gaaagcgcgc cgtcgtgtcg
     1141 aagcaaacgg tttttcaata gcagttttcc cagtgtcgtg tagaagaagt acggcaccgg
     1201 atcccgatgc agatcgatca cgaaaatgcg gcgtcgcgct actcgattca tctcgcgtaa
     1261 aatatgcacg acctgttcgt cgacgaagtg gtgcgtgaag agcgaacaca tcacgtaatc
     1321 aaactgcgac tccatgaagg gtagtcgcaa cgcgtcgcca cgcacggaag ttatcgccgg
     1381 aaaatccttc gactcctcga gtatcgcctg cgcggatcgt tcgtttagtt cgagtccaac
     1441 gcccttgaaa ccgcgacgag tcgctctggc ccaagtggca gcaacacgca gcagttcgcc
     1501 tgaacctgcc ccaacgtcga gcaccgtaaa ctcacgcaag cctttttctt caacctctct
     1561 gagcagcgtg tgtttgagag catgtgcgtc gcccatccaa cgattaacga gttggagttc
     1621 ggaaatacag tgttcgtact cttctggcgt gtagttgccg gtgtcgatgt attcgagctc
     1681 ggagctgcgc cgcttgaact tgtcgaacat ttctattggt cagttgtccg tggtcatttg
     1741 tctgtcgtta gtgtctcagc acggaccatg gactactgac cactgccgga attaatcctc
     1801 tggaccacga gcgcactgtt tttcgagcca aagccgatac agttgcagag cgctgtttcg
     1861 acatttgcct cacgtgcttc gttggggacg tagtcgagat cacaatcggg atcgggttct
     1921 tcgaggttga tggtcggagg aatcttgttg gtgtgcatcg cacacaacgc cgcgccgagg
     1981 cccgcagcgc cgctcgcgcc ctgcggatga ccgatctgcg atttcgtcgc cgacattgga
     2041 atgcgcgccg catggccatc gaaggccagt ttcaacgcgc tggtttcaat gcgatcgtta
     2101 agcacggtcg aggtgccatg caggttaacg taatcaatgt ctttcgcttc gagacccgcg
     2161 tcgtggagcg cgaggcgcat cgcgcgcgct ggttcgtcac cgtcttcggc gagacgtacg
     2221 cgatgatatg catcgcaggt cgcgccgtag ccggtaatct cggcgtaaat ttgcgcgccg
     2281 cgactggtcg cgtgtttcag ttcttccaac acgtaaatcc acgagccctc gccgagcacc
     2341 atgcccgacc gattacgtga aaacggtcgc gaggcgcgct gaggttcgtt attccaatcg
     2401 ttcgtcaaca cagtcatcag attgaacgcc gcgagaatcc cgggcgcgat cggcgcgtcg
     2461 acgccgccgg cgagcatcag gtcctgacgg ccgagtgcga tgtgttgggt cgcatacgcg
     2521 atcgcgtcgg tggagctggt gcaaccggta gaaacaatgt gcgatagacc acgaagtcca
     2581 aacgccatag acaactcgct ggatagacca ccgtgcgtcg aagcgggaat ggtgtagacg
     2641 ctcgctttgt gggttggacc aacgtaccag tactcatact gctgttcggt gaacgccaga
     2701 ccaccaccgc ccgtaccgag ttcaacaccg aaagcgcgcc gttcgtcgag cgacatgtct
     2761 tcgggaaata aacgagcgtc ggctagggct tcacgcgctg cggcgagagc aagcggaacg
     2821 gtgcgcggta cgtgtttgcg atcttttgga tttagttggg cttcccagtc gaaggaagga
     2881 acttcggcgg cgatttggac cgggaaactg gacggatcga aggactcaat tctgcgcacg
     2941 ccgctcttgc cggcgacgag tgcgtcccaa aaagcttcgc gtccgattcc gagaggggtc
     3001 acacacccca ttccggttat aacaacccgg cgtggacgcg tgaaccctct cagcattcag
     3061 cttaacctcg gtgtttagac tttctggcca cactcgttgc agaacttcgc gccgggaggc
     3121 aattgggcct ggcagtttgg acacgttgcc gcaccgagct tcgtgccgca ttccacacag
     3181 aacttcgcgc caggactgtt gttgttacca cactgcggac aagtaacacc cgcagccgcg
     3241 ccgccgccgg ccgctgttgc cgtaccaccg cctccgcctt ctttcgattg gcggatggag
     3301 tctgtcattg cgcttgccat cgcgtttcca acggcaaagc cggcgccaag tccagcgccc
     3361 gtgccggcgc cgccggaagg attctgtgcc gcatcgcgca ttgcatcagc ggcctgaaat
     3421 tgtgtgtagc ggccaacatc gccgatgaca cccatcgacg tgcgtttgtc catcgcggat
     3481 tcgacttcct gaggcagcga gatgttctca atgacaaact tcgtcaactc gagtccccag
     3541 gctttgaagt cttcgttcat cttgccacgc atgaacttgc cgagttcatc gtagtttgac
     3601 gcgaggtcga gcgcggcgat tttggattcg cccagcgcat cgctgaagct gctcaccagc
     3661 gtgcgcttca gttggccttc aacgtcctcc gtaacgaagt gcgcgttcgt gccggcgat
//
LOCUS       DASTEY010000318         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_929654, whole genome shotgun sequence.
ACCESSION   DASTEY010000318 DASTEY010000000
VERSION     DASTEY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929654"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFO72_05615"
     CDS             <1..216
                     /locus_tag="VFO72_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478798.1"
                     /translation="RLTGEYGFRVLNASRSVRTVAADLRRAIVNVLEEPAPPAGENVS
                     HIQPLRNKAAPAKPATNLLDLNKKALP"
     gene            213..1178
                     /locus_tag="VFO72_05620"
     CDS             213..1178
                     /locus_tag="VFO72_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET9478799.1"
                     /translation="MKLAAIDIGSNSIKLVVVEAQTSDSFAVLASDRQVVRLGQETLV
                     NGYISSEAMLRAISWLKRFRSAAEAHGAEKIATIATASVREASNSLEFIRMVEQETGL
                     RVEVLSGIEEARLIGLAASQGCTAKGVTSLNIDIGGGSTEVSIFRDGEPLKLVSIKLG
                     AVGLTDRFVRSDPPGPDELKEMRSAIRVSLERHVRELREQSWDDATATSGTALAIGAA
                     LNHTTLGASSTIALAELKRLNERLASLSIAERRTVAGLSPQRADIIVAGGFVLEGVMT
                     ALDIESSRVCDWSLREGVIIDRLRQWERAQGSMARFAGEKIDGVG"
     gene            complement(1134..1625)
                     /locus_tag="VFO72_05625"
     CDS             complement(1134..1625)
                     /locus_tag="VFO72_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET9478800.1"
                     /translation="MMSTPIRTAVITASDACSRGERPDDSGAALVELLAGSGAEVVET
                     RILSDDLGPLVQSLEELARRDDINLILTTGGTGLGPRDNTPEATRQVIEREAPGIAET
                     IRAESLKVTPMAMISRGVAGVASGTLIINLPGSPKAVRESFAVIKPVLAHAVDLLAGK
                     TRH"
     gene            complement(1695..2189)
                     /locus_tag="VFO72_05630"
     CDS             complement(1695..2189)
                     /locus_tag="VFO72_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9478801.1"
                     /translation="MTSEQGKTSVQINSADTIRRILDECSTIAVVGLSSDTLRPSNSV
                     SAYMRRVGYRVIAVNPNETEVFGEKAYPDLAAVPEKIELVDVFRRSDEAGKAVDEAIA
                     VGAKAVWLQEGVIDNAAAQRALEAGLLVVMDRCWLKEHIRYAGADLEPRDPDNLGPQT
                     SRLN"
     gene            complement(2186..>3926)
                     /gene="carB"
                     /locus_tag="VFO72_05635"
     CDS             complement(2186..>3926)
                     /gene="carB"
                     /locus_tag="VFO72_05635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9478802.1"
                     /translation="TEVSEDEVRARRKELGIVPVYKRVDTCGAEFESYTPYLYSTYET
                     EDESAPTDRRKIMILGSGPNRIGQGIEFDYCCCHASFALHEAGFETIMVNCNPETVST
                     DYDTSDRLYFEPLTFEDVMNIVDVEKPEGVIVQFGGQTPLNLAMRLHKAGVRIIGTSP
                     DSIDLAEDRKQFGALLKELGIPQPDHGMAASVEEAREVAGRIGYPVLVRPSYVLGGRA
                     MAIVYDQEQLEKYVLTAVGFSPDRPVLIDKFLEQAAEFDVDALADETACVIAGIQEHI
                     EQAGIHSGDSSCVLPPVRIDPEHLETMRHYTKKLARALSVRGLMNIQFAIKDHRVYVL
                     EVNPRASRTVPFVSKATGVPIARIAALVMAGQKLSDFNLPEELTVPRFFIKSPVFPFA
                     KFPGVDPILSPEMHSTGEVMGIGDTFGEAYGKAMMGAGTSLPSTGKAFMSVNDADKGT
                     AVLLARKLSRLGFELVATLGTAARLREVGLTVENVFKVNEGRPNIADYIKSGDISLVI
                     NTPLGRVSRFDEQAIRRAALQFNVPCVTTMTGAQALVEAIAANRVGSTPNVHSLQELH
                     AIRESMDAVSSAQ"
BASE COUNT          836 a         1244 c         1033 g          813 t
ORIGIN      
        1 cgcctgaccg gcgaatatgg attccgcgtg ttgaatgctt cgcgatccgt tcgcaccgtg
       61 gccgccgatc tccgtcgcgc catcgtgaac gtgctcgagg aaccggcgcc acctgcggga
      121 gagaatgtca gccacataca accgctgcga aataaagccg cgcctgcgaa acctgccacc
      181 aatctgctcg acctcaataa aaaggcactc ccttgaagct cgcggcgata gatatcggtt
      241 cgaattcaat caaactcgtc gtggtcgaag ctcagaccag cgattcgttc gcagtgctcg
      301 cgagcgaccg gcaagttgtc aggttgggcc aggagacact cgtcaacggc tacatcagta
      361 gcgaggcgat gctgcgcgcg atttcgtggc tcaaacgttt tcgatcggct gccgaggccc
      421 acggcgccga gaagatcgcc acgatcgcga ctgcatcggt acgcgaagcc agcaattctc
      481 tcgaatttat tcgcatggtg gaacaggaga cgggtctgcg tgttgaggtg ctttcgggca
      541 tcgaagaagc gcggctgatc ggccttgcag catcacaagg ttgcactgca aagggagtga
      601 ccagtctgaa catcgatatc ggcggcggct ccactgaggt ttcgatcttt cgcgacggcg
      661 aaccactcaa actcgtctcg atcaaattgg gagcagtcgg cctgacggac cgattcgtcc
      721 gttcagatcc gcctggccca gacgaactga aagaaatgcg ctcggcgatc cgtgtttccc
      781 tcgagcgtca cgttcgcgaa cttcgagaac aaagctggga cgacgcaacg gcaacgtccg
      841 gaacggctct ggcaatcggc gccgcactaa accacaccac gcttggcgct tcatccacaa
      901 tcgctcttgc tgaactcaaa cgtcttaatg aacggcttgc gtcgctcagc attgccgagc
      961 gtcgaacggt ggcgggccta tcgcctcaac gagcggacat cattgttgcc ggcggcttcg
     1021 ttctcgaggg agtgatgacg gcgcttgata ttgaatcgtc gcgagtgtgc gattggtcgt
     1081 tgcgcgaagg cgtgattatc gatcgcttgc gtcagtggga acgagcgcag ggttcaatgg
     1141 cgcgttttgc cggcgagaag atcgacggcg tgggctagga ctggtttgat aacggcgaaa
     1201 ctttcgcgca ctgctttcgg cgaaccgggc agattgataa tcagagttcc tgacgccact
     1261 cccgcaacac ctcgcgagat catggccatc ggcgtcactt tcaacgactc agcacgaatc
     1321 gtttccgcaa ttccaggcgc ctcacgttcg atcacctgcc gcgtggcctc gggcgtgtta
     1381 tcgcgtggtc caagaccggt gccgccagtc gttagaatta aatttatatc gtcgcgcctc
     1441 gcgagctctt cgagagattg aaccagtggc ccaaggtcgt cggaaagaat ccgtgtctca
     1501 accacctctg caccggaccc cgccagcagt tcaaccaacg ccgcacccga atcatccggc
     1561 cgctctccac gcgagcaagc atcactggca gtaatcactg ccgtccgaat cggagtgctc
     1621 atcatctagt ctcgtacctt cttcctggga ccgcaggcgt ctcgcctgcc cgcaaccatt
     1681 ttcgcgctcc gcgctcagtt caggcgagac gtctgtggtc ccaggttatc agggtcccta
     1741 ggttccagat cagcgcccgc gtatctgatg tgttctttca accagcagcg atccattacg
     1801 accaacaacc ccgcttcaag cgcacgttgc gcggcggcgt tgtcaatcac tccttcctgc
     1861 aaccacaccg ccttcgcgcc gaccgcgatc gcttcgtcaa cagccttccc cgcctcatcc
     1921 gatcgccgga acacatccac caactcaatc ttctccggca cggccgccaa atcgggatac
     1981 gccttctcgc caaagacctc cgtctcgttt ggattgacgg cgatcacgcg gtaacccacg
     2041 cgccgcatgt acgccgacac actattcgac ggtcgcaacg tgtcggacga aagtccaacg
     2101 actgcgatcg tactgcactc gtctagtatg cgacgaattg tgtcggccga gttgatctgc
     2161 acggaagttt tcccttgttc gctagtcact gggccgacga aaccgcgtcc atcgattctc
     2221 ggatcgcatg caactcctgc agcgagtgca cgtttggagt gctcccgact cgattcgccg
     2281 caatcgcttc taccagcgcc tgcgcgccgg tcatcgtcgt cacacatggc acattgaatt
     2341 gcaacgccgc gcgccgaatt gcctgttcgt caaaacgcga gacacggccc agcggcgtgt
     2401 tgatgacgag cgagatgtcg ccactcttga tatagtcggc aatgttcggc cggccctcat
     2461 tgactttgaa aacattctca accgtcaacc caacttctcg caaacgcgcc gcagttccca
     2521 gcgtcgccac caactcgaaa ccgagccggc tcaacttgcg tgcgagcaac accgcagttc
     2581 ctttgtccgc atcgttgacg ctcatgaacg ctttgccggt cgacggcaaa ctcgtaccag
     2641 cgcccatcat cgcttttccg taagcctcgc cgaatgtgtc gccgatgccc atcacctcgc
     2701 cggtcgaatg catctcggga ctcagaatcg gatccacacc gggaaacttt gcaaacggaa
     2761 acacaggcga cttgatgaag aagcgcggca ccgtcaactc ttccggcagg ttgaaatctg
     2821 acaacttctg tccggccatc accagcgccg caatccgcgc gatcgggact cccgttgcct
     2881 tcgagacaaa cggcaccgtg cgcgacgcgc gtggattcac ttccaacacg taaacgcggt
     2941 ggtccttgat ggcaaactga atgttcatca aaccacgcac cgacaacgcg cgcgccaact
     3001 tcttcgtgta gtgacgcatg gtttccaggt gctcgggatc gattcgcacc ggcggcagca
     3061 cgcatgacga atcgcccgag tgaatcccgg cctgttcgat atgctcctga attcccgcaa
     3121 tcacgcacgc cgtttcatca gccaacgcgt cgacgtcaaa ctctgccgcc tgctccagaa
     3181 acttgtcgat caaaaccggg cgatccggtg agaaaccaac cgccgtgaga acgtattttt
     3241 ccaactgctc ctgatcgtaa acgatggcca tcgcgcgccc acccagcaca taactcggcc
     3301 gcaccagcac gggataaccg attcggccgg caacttcgcg cgcttcttca accgacgccg
     3361 ccatgccgtg atcgggctgt ggaataccaa gttctttcag caaagctccg aactgtttgc
     3421 gatcctcagc gaggtcgatc gaatccggcg acgtaccgat gatcctgacg cccgctttgt
     3481 gcaaccgcat tgccagattc aacggcgttt ggccgccaaa ctgaacgatg acgccctccg
     3541 gtttttccac gtcgacgatg ttcatcacgt cttcaaacgt gagcggctcg aagtagagac
     3601 gatccgacgt gtcgtaatcc gttgagacag tctcgggatt acagttgacc atgatcgttt
     3661 caaagcctgc ctcgtgcagc gcaaacgacg cgtggcaaca acagtagtca aactcaattc
     3721 cctgcccgat gcggtttgga ccagatccga gaatcatgat cttgcgccgg tcagtcggcg
     3781 ccgattcatc ttcggtttcg taagtcgaat agaggtaagg cgtatatgac tcaaactcag
     3841 cgccgcacgt gtcgacgcgt ttgtaaaccg gcacgatgcc caactctttg cgacgcgcgc
     3901 gcacttcgtc ttcggaaact tctgtc
//
LOCUS       DASTEY010000319         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_418921, whole genome shotgun sequence.
ACCESSION   DASTEY010000319 DASTEY010000000
VERSION     DASTEY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418921"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1485
                     /locus_tag="VFO72_05640"
     CDS             88..1485
                     /locus_tag="VFO72_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9478803.1"
                     /translation="MMKKLLTLLLISFVGSVAQAQRVAPQETLIRNATVLTITRGTLQ
                     NTDVLLRNGKIAGVGKNLNASANARIIDGTGKYVMPGIIDAHSHSMLDTINEGSLAVT
                     SMVRTEDVLNPTDVDLYRGLAGGVTTLNLLHGSANPIGGLNTVVKIKYGRPASEFLFP
                     GAMPGIKFALGENVKRSSTPNQPGVTRRYPNTRMGVEETIRDAFVRARDYKRAWDEYR
                     AAAGKDKNLIPPKRDLQLEPLVEILEGKRYVHAHCYIASEILMLINLADEFGFKIKTF
                     QHVLEGYKVAKEIAAHGAGASIFADSWSYKIEAYDAIPYNAAIMTRAGVVVSMNSDSD
                     ERARRLNIEAAKAMHWGDLTEEQALRLITINPAIQLGVQDRVGSIEVGKDADLALWNG
                     HPFSVYSRVDTTFVDGIVFFDREQDLARRPELAKERAALEQAEPNRPPAQGQSPQAPR
                     RRRPTQDDTIEEEQP"
     gene            1482..>2300
                     /locus_tag="VFO72_05645"
     CDS             1482..>2300
                     /locus_tag="VFO72_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478804.1"
                     /translation="MNLLTRFANVALVMSICVICGQTVVGQQIGNPTTQQGVLPPRGV
                     YAIRNARIVTVSGPDIENGTVVIRDGKIEAVGAAVNVPTGAQTIDGRGLSVYPGMIDA
                     GTNMGLVEVPQGANGTVDLSEVGDFNPNAKAIVAVNPHSAHIAVTRVEGVTNTLTAPT
                     GGLISGQAALINLLGTAPKEMAVLPQAALVINYPRVAGGGGGFGGFQQQQRSLSETLE
                     ANARQVEQIRKMLRDAEAYGRAQDAYAKDRSLPRPDRNIVLESLVPYVRGEQPVI"
BASE COUNT          540 a          654 c          668 g          438 t
ORIGIN      
        1 cgtggaactg tgacggtcgt tggccttgcg cccggatcat tcacagcaac ccgatcgcgt
       61 ccaaactgaa ccacagaggg aacacagatg atgaaaaaac tgctaactct attactgatt
      121 tcgttcgtgg gatctgtggc ccaggcacaa agagtggcgc cgcaggaaac gctgatacgc
      181 aatgcgacgg tgctcaccat cacgcgcggc acgttgcaaa acaccgacgt gcttttgcgc
      241 aacggcaaga ttgccggcgt cggtaagaac ctcaacgcct cggcaaacgc gcgcatcatc
      301 gatggcaccg gcaaatacgt catgcccgga atcatcgacg cccactcgca ctcgatgctc
      361 gatacgatca acgaagggtc gctcgccgtc acgtcaatgg tgcgaaccga ggacgtattg
      421 aacccgactg acgtcgatct ttatcgcgga ttggccggtg gcgtgacgac gttgaacctg
      481 ctgcacggct cggcgaatcc gattggcggt ttgaacacag ttgtgaagat caagtatggc
      541 cgtccggcga gcgagtttct gtttccaggc gcgatgccgg gcatcaagtt tgcgctcggc
      601 gaaaacgtga agcggtcaag cacgccgaat cagccgggcg tcacgcgtcg ttatccaaac
      661 acgcgcatgg gcgttgaaga aacgattcgc gatgcgttcg tgcgcgctcg cgattacaag
      721 cgagcgtggg acgagtatcg cgccgcggca ggaaaagaca agaacctgat tccaccaaaa
      781 cgcgatcttc aactcgagcc gctcgtcgaa atcctggaag gcaaacgtta cgtgcacgcg
      841 cactgctaca tcgccagtga gatcctgatg ctgatcaatc tcgctgacga gtttggtttc
      901 aagatcaaaa cctttcagca tgtgctcgaa ggttacaagg tggcaaaaga gatcgccgcg
      961 catggcgcgg gcgcttcgat ctttgccgat tcgtggagct acaagattga agcttacgac
     1021 gccattcctt ataacgctgc catcatgact cgcgcaggag tggtggtttc aatgaactcc
     1081 gattccgacg aacgcgcgcg tcgtttgaat atcgaagctg caaaagcaat gcactggggc
     1141 gacttgactg aagaacaagc actgcggctg atcacgatca atcccgccat acaactcggt
     1201 gttcaggacc gcgtcggctc gatcgaggtc ggcaaggatg cagacctcgc gctctggaac
     1261 ggacatccgt tcagtgttta ttcgcgcgtc gacacgacat tcgtcgacgg catcgtcttc
     1321 ttcgatcgcg agcaggatct cgcgcgccgt ccggagctcg ctaaagaacg tgcggctctg
     1381 gaacaagccg aaccaaacag accaccggca caaggacagt cgcctcaggc gccacgtcgg
     1441 cgacgaccaa cccaagacga cacaatcgag gaggagcagc cgtgaacctt ctaacgagat
     1501 ttgcgaacgt tgcactggtc atgtcgatct gtgtgatctg cggccagacg gtcgtgggcc
     1561 aacaaattgg caacccaacc acgcaacaag gcgtgctgcc accgcgcggt gtgtacgcga
     1621 ttcgtaatgc acggatcgta actgtaagtg gtccagatat cgagaacggc acggttgtga
     1681 ttcgcgacgg caagatcgaa gcagtcggcg ctgccgtaaa cgttccgaca ggcgcgcaga
     1741 cgatcgatgg ccgcggtttg tcagtttatc caggcatgat cgatgcgggc acgaacatgg
     1801 gccttgttga agtgccgcag ggcgctaacg gcacggttga tctatcggaa gttggtgact
     1861 tcaaccccaa cgccaaagcg atcgtcgctg tgaacccaca cagcgcgcac atagcagtca
     1921 ccagagtcga gggtgtcaca aatactttga ctgcgccgac tggtggtttg atctccggcc
     1981 aggcagcgct gatcaacctg ctcggcaccg cgccaaaaga gatggcggtc ttgccgcagg
     2041 cggcacttgt gatcaactac ccgcgcgtcg ccggtggtgg tggaggtttt ggcggcttcc
     2101 aacaacagca gaggagccta tcggaaacac tcgaagccaa tgcgcggcaa gtcgagcaaa
     2161 ttcgcaagat gttgcgcgat gccgaagcgt atggccgcgc tcaggacgct tacgcgaagg
     2221 atcgatcgtt gccgcgtccc gatcggaaca tcgttctcga atcactcgtg ccttacgttc
     2281 gcggcgaaca accggtcatc
//
LOCUS       DASTEY010000320         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_501356, whole genome shotgun sequence.
ACCESSION   DASTEY010000320 DASTEY010000000
VERSION     DASTEY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501356"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFO72_05650"
     CDS             complement(<1..537)
                     /locus_tag="VFO72_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9478805.1"
                     /translation="MAIVKDAEFTEAIETTDNDQQLQIPNELPVLPLRDIVIYPFMIV
                     PLFVSREKSIRAVDEALAENRMILLASQKDLDKEEPTAEDLYSVGTAAVIMRMLKLPD
                     GRIRILVQGLSRAHIESVDATGEYLRSRVSVMQEVLPPERSLEVEALVRNVRASMEKA
                     INLGKNISPEVMAIIANLD"
     gene            complement(550..1023)
                     /locus_tag="VFO72_05655"
     CDS             complement(550..1023)
                     /locus_tag="VFO72_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9478806.1"
                     /translation="MIKPVRATGLERLELQRLRDRVGRLYMALEEATEADNPLASGAW
                     APPVDLCETEKMIFLRVELPGVNGEQIKIGRPNTKLRSWGEKKRRPARRRIISYICSE
                     RSYGKFNRVVRLRWTICVRTASAELKNGMLHIYLPKIEDRRGAEVMIAVKDASTE"
     gene            complement(1033..1491)
                     /gene="nrdR"
                     /locus_tag="VFO72_05660"
     CDS             complement(1033..1491)
                     /gene="nrdR"
                     /locus_tag="VFO72_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET9478807.1"
                     /translation="MKCPFCGYLEDKVVDSRESREGDAIRRRRECLRCERRFTSYERI
                     DEIPYMVVKKDGRRETFDRNKVMAGLLRACEKRPVASAKLESIVNAIEKYVQESPERE
                     RPTSKIGEMIMRRLKELDKVAYVRFASVYLEFEDVSEFMTELKQLVQKRR"
     gene            complement(1551..2591)
                     /locus_tag="VFO72_05665"
     CDS             complement(1551..2591)
                     /locus_tag="VFO72_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9478808.1"
                     /translation="MKPKSYRRYIEFLALCLLAAALLWWFGRNLDWREVRHALGTADP
                     LLLTLAILIICVAYLARAFRWGALLKPLVSARLADLFAATTIGFSAVFLIGRAGEVVR
                     PVVLPMRDPQVRPSAALVTILIERIYDFVAVALLFAINLIWFQPPVALEVSFSRVRTV
                     GFALLGGTILGIAFLIWFRKNSTTLLGALNGFFSRSRFFPQRLARLVIRILEQLSSAL
                     RVLVNASELAETVGWTALLWLGISSANLLVLRAFHLDVGMSETIFILGWSLLGSLVPT
                     PGGAAGAFHAATAAGLLFLGVKKETAAALSIVLHIVDFGPAVLFGLFYFIRGDLSISR
                     LRTLISPAGAEL"
     gene            2845..3438
                     /locus_tag="VFO72_05670"
     CDS             2845..3438
                     /locus_tag="VFO72_05670"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9478809.1"
                     /translation="MKALAVSEGAELVRRCRAGDGAAWEEIVQTYSRRIYNLAYRFTS
                     RADTAEDLTQEVFVRVYRSLDQYDPKQGDLQNWLMRLARNLIIDDYRKRQRTPHDNQA
                     DDLADHSYHLHTRTNSVQTEMERRELGAQVQAGIDRLSPDLRTCVILRDIEELSYQEI
                     VDLLKIPEGTVKSRINRGRIELAKILRRMKVVAMSEV"
     gene            3484..4302
                     /locus_tag="VFO72_05675"
     CDS             3484..4302
                     /locus_tag="VFO72_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9478810.1"
                     /translation="MLCVDFETLLTDYIEGVVGPETNRQMAEHAMKCPLCHETLSAVK
                     STVAACRVAAVPEPSRDLDARILQRTVPETAMTCEEFEEFLTDYLDGFLPAHLYHRWE
                     RHAAVCERCTELPGEVVRSIGACYTYISEEKPLPAGLHEKIMQATLGTATPREIKAPW
                     TVRAASRFRLWLDPIISPQLASVATMILVAVLVLTNTVSADGSISGVYNAGLALAERT
                     ASPSQMLPGRLQQIDADREQTPPAPPAQKQNGDQHNESTGGGGKPENNGQKNRR"
     gene            4451..5080
                     /locus_tag="VFO72_05680"
     CDS             4451..5080
                     /locus_tag="VFO72_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478811.1"
                     /translation="MLRHSHASPSHILRRKSSPFVATLLSFVPGLGAAYNGQTSKAIV
                     HFAIFASFFQMAVVTQGMYFFILGVIGTWLFAAVDACRTAQLMRAGLSPDAEDDVIAR
                     RLYGNPLAWGTTLIIIGTLFLLHTLLRVTLPIKELLPVALVALGAYMLFDYIRRSKSS
                     DRIVRFDSRRPPPSVVAGSRPTDIPNFRTGETTQLSRRDVTPLPFERRS"
     gene            5114..5335
                     /locus_tag="VFO72_05685"
     CDS             5114..5335
                     /locus_tag="VFO72_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478812.1"
                     /translation="MCCGVVKAFGGLGAVIALILLIVALLKQLIGLVAFLLTLVKIAI
                     VVVFIAVLVMIVLAIFRDRARRRRDLEDV"
BASE COUNT         1259 a         1547 c         1547 g         1237 t
ORIGIN      
        1 gtcgaggtta gcgatgatgg ccatcacttc gggcgagatg tttttgccga gattgattgc
       61 cttctccatc gaagcgcgca cgttgcgcac cagcgcttcg acctcgagcg aacgttccgg
      121 cggcagcact tcctgcatca cgctcactcg cgaacgcaga tactcgccag ttgcgtcgac
      181 agattcgatg tgtgcgcgag agagaccctg caccagaatg cgaatgcggc catcgggcaa
      241 cttcaacatg cgcatgatga ctgccgcagt gccgaccgaa taaagatcct cagcagtggg
      301 ttcttcctta tcgaggtctt tctgcgacgc cagcagaatc atccggttct cggccagtgc
      361 ctcgtccacc gccctgatcg atttttcgcg cgacacaaag agaggcacga tcatgaacgg
      421 gtagatcacg atgtcgcgca aaggcagcac cggcagttca ttcggaattt gtagttgctg
      481 atcgttgtcc gttgtttcga ttgcttccgt gaactcagcg tcttttacta ttgccatgat
      541 gtaagttccc tactccgtac tcgcgtcctt gactgcaatc attacttcgg cgccgcggcg
      601 atcttcaatc tttggcaggt agatgtgaag catgccgttc tttaattctg ctgaagcagt
      661 gcgaacgcag atggtccagc gcaaacgtac tacgcgattg aacttgccat aactgcgttc
      721 ggagcaaatg tatgagatga tgcgccgccg tgccggccgt ctcttcttct cgccccagct
      781 tctcaacttc gtgttaggcc ggccaatttt tatctgctca ccgtttaccc ccggcagttc
      841 aacgcgcagg aagatcatct tttctgtttc acatagatcc accggcggcg cccacgcgcc
      901 cgaggccagc gggttgtctg cttcagtggc ttcctccaaa gccatataca gacgacccac
      961 tcgatcgcgc agacgctgca attcaagtcg ttctaatccc gttgctcgta caggtttgat
     1021 catgagaaat cctcaacgcc gcttctgaac cagttgcttt agttcggtca taaactccga
     1081 tacgtcttca aattcaagat aaaccgaagc gaagcgaaca tatgcgactt tatcgagttc
     1141 tttcaagcgc cgcatgatca tctctccgat tttactcgtg ggccgttccc gttctggtga
     1201 ctcctgcaca tacttttcga ttgcattcac gatgctttcg agcttggccg aggctaccgg
     1261 acgtttttca cacgcgcgca gcaggcccgc catcactttg ttgcgatcga aagtttcgcg
     1321 gcggccgtct ttctttacca ccatgtaggg tatctcatcg atacgctcgt aagaggtgaa
     1381 gcgtcgttca caacgaagac actcgcgccg gcgccgaata gcgtcgcctt cgcgcgactc
     1441 gcgcgaatca actactttgt cttcgaggta tccacagaaa ggacatttca tattaaccct
     1501 ccaaggtaga acagactttc cagtctgtct gacaggcaag aatgcctgtc ctacagttcc
     1561 gcgccggcag gcgagatcag cgttcgtaat cgtgaaatac tcaaatcgcc gcgtatgaaa
     1621 tagaagagtc caaacaatac cgctggtcca aagtcgacga tgtgcaaaac gatggaaagg
     1681 gccgccgcag tctctttctt gacgccgaga aacaacaacc ccgctgccgt ggctgcgtga
     1741 aaagcacccg cggctccgcc cggcgtcggc accagcgatc ccaatagcga ccaccccagg
     1801 ataaagatcg tttccgacat gccgacgtcg agatgaaacg cgcgcaacac aagcaggttc
     1861 gcgctggaga tcccgagcca aagcagtgca gtccaaccga ccgtttccgc cagttcagac
     1921 gcgttcacca gcactcgcaa cgcactcgat aactgctcga gaattcggat tacgagtctt
     1981 gccagtcgct gggggaagaa ccgcgagcgg gaaaagaacc cattgagagc accgagcagt
     2041 gtcgtcgagt tttttctgaa ccagatcaga aacgcgatgc cgagtattgt gccacccagg
     2101 agtgcgaacc ccaccgttcg cacgcgactg aacgagactt caagggcaac cggcggctga
     2161 aaccagatca gattgatcgc aaacaacaac gcaacggcga cgaagtcgta aattcgttcg
     2221 atcaggatcg tgaccagcgc tgcactcggc ctgacctgtg gatcgcgcat cggcagaaca
     2281 accggacgaa cgacttcacc cgcgcggcca ataagaaaca cggcgctgaa accgatcgtc
     2341 gtggccgcaa aaaggtctgc cagacgcgcc gagactaacg gtttcagcag tgcaccccag
     2401 cgaaaagccc gcgcgaggta agcgacacag atgatcaaga tagcaagcgt gagcaggagt
     2461 ggatcggcgg tgcccaaagc gtgacgcact tcacgccaat cgagattacg gccaaaccac
     2521 caaagcagtg ccgccgcgag caggcacagc gccaggaact cgatatatct gcgatagctt
     2581 ttgggcttca actctcttgc gggccgaacc acaacatgtt gggtcggccc gagagcctac
     2641 tacagcgaac gagtctgagc aagaaaccag atctgtgtct tcggtggaga tccgggttgc
     2701 aaggctgttc gatagcgaat ttttggatta aagtctcaac gggaacttta atttgacggc
     2761 tgtcgtaaac gcctgatgtc ataggttcgc tgcctttgac gagttcccga agcacaggta
     2821 cctggagaag atacggtggg ggctgtgaaa gctttggcag tcagcgaggg agccgaattg
     2881 gttcgcaggt gtcgcgcggg cgatggcgcc gcatgggaag agatcgtcca gacctattcc
     2941 cgccgcattt ataaccttgc ttaccgcttc acctcccgag ctgacaccgc agaagacctg
     3001 acgcaggagg ttttcgtcag ggtctatcgc tctttagatc aatacgaccc gaagcagggc
     3061 gacttgcaga actggctcat gcgactggcg cgaaatctga ttatcgacga ctatcgcaaa
     3121 cgccagcgta cgccgcacga taaccaggcc gacgatctcg ccgaccacag ttatcacctg
     3181 catacgcgca cgaactcggt gcagacggag atggaacgtc gcgaactcgg tgcgcaggtc
     3241 caggctggaa tcgacagact ctcgcctgat ctgcgcactt gcgtgatcct gagagacatt
     3301 gaagaactga gctatcagga gatagtcgat cttttgaaaa taccggaagg gacagttaag
     3361 tcacgcatca accggggccg gatcgaattg gcgaagattc tccggcggat gaaagtagtg
     3421 gcaatgagtg aagtgtaaac gaggggacgg aagttaacag aagcggcagg aaagcgaaac
     3481 gtgatgcttt gtgtggattt cgaaactcta ttgactgatt acattgaggg cgtagtcggt
     3541 cctgaaacca atcgccagat ggcggagcac gcgatgaagt gtccgctgtg ccatgagacg
     3601 ctgtcggcag tcaaaagcac tgtggcagcg tgtcgagttg cagccgtgcc ggaaccatcg
     3661 cgcgatctcg atgcgcgtat tttgcagaga acggtgcccg agacggcgat gacttgtgag
     3721 gagtttgaag agttcctcac tgattacctt gatggcttcc tgccggctca tctctatcat
     3781 cgttgggaac gtcacgcggc cgtgtgtgag cgctgcaccg aacttccagg tgaagtcgtt
     3841 cgctcaatcg gcgcgtgtta cacctacatc tcggaagaga aacccttgcc cgccggtttg
     3901 catgagaaga tcatgcaagc cactctgggt actgccacgc cgagggagat caaggcgccg
     3961 tggacagtac gcgccgcttc gcggttccgc ctctggttgg atcccatcat ctcgccacaa
     4021 ctcgccagcg tggctacgat gatactcgtg gcggtcctcg tgctgaccaa cacagtttct
     4081 gctgacggtt cgatcagcgg tgtttacaac gcgggactgg cgctcgccga acgaacggcc
     4141 tcgccgtctc agatgctccc cggaaggctg caacagatcg acgcggaccg ggagcaaaca
     4201 ccgccagcgc ctcccgcgca gaagcagaac ggcgatcaac ataacgaatc gactggcggc
     4261 ggcggaaaac ccgagaacaa cggtcagaag aacaggcggt gaggaaatcg agatgatgta
     4321 ctgtagctac catgcaaaga atcccgcggt ggttcagtgc aatcaatgcg cgcggtggtt
     4381 atgtccggct tgtgatcatc gcgtgcgcgg atttccgttt tgccaggatt gcatcgtagc
     4441 cggagttgaa ttgctgcgac attcgcacgc cagtccatca cacattctgc gacgaaagag
     4501 ttcgccgttt gtcgcgacgc tgctttcgtt cgtacccgga ttgggcgctg cttacaacgg
     4561 gcagacgtcg aaggcgatcg ttcacttcgc aatcttcgcc agcttctttc aaatggccgt
     4621 cgtgacgcag gggatgtact tcttcattct cggcgtgatt ggaacgtggc tgtttgcggc
     4681 ggtcgacgct tgcagaacgg cgcagttgat gagggctggg ttgagtccgg atgctgaaga
     4741 cgatgtgatt gcgcggcgtc tttacggaaa cccgctcgcc tggggcacga cactgatcat
     4801 catcggcacg ctcttcctgc tgcacacgtt gttgagagtg acgctaccta taaaagaact
     4861 gctgccggtt gcactggttg cccttggcgc ttacatgctg tttgactaca ttcgccggag
     4921 caagtcgtcg gatcgaattg ttcgtttcga ttcgcggcgt ccgccaccgt ccgttgttgc
     4981 cggttcgcgg ccgactgaca tacccaattt tcgcaccggt gaaactactc agttgtcgcg
     5041 gcgcgacgta actcccttgc ctttcgaacg tagatcgtaa taattggtgc ggtaatagta
     5101 acgaggtaaa tacatgtgct gtggcgttgt taaagctttt ggcggactgg gcgccgtgat
     5161 tgcgctgatt ctcctaatcg tcgcgctgtt gaagcagttg atcgggctgg ttgcttttct
     5221 cctgacgctt gtaaagatcg cgatcgtggt ggtgttcatt gccgtgctgg tgatgatcgt
     5281 gctggcgatc ttccgcgacc gcgcgcgcag acggcgagat ttagaagacg tgtaaacgca
     5341 gattctatct gtgtgatggg gcgcttgctt ttttcttgca aagttgcaac tcctcacaag
     5401 gctgtgatag tttcagggcc ttgcagaacc ggccggatgg atttcacctc ggagggacct
     5461 tgaggttttc aatccacccg ctgcaaatct tcaagtagga aacaaacaaa actttcccgt
     5521 aaccgcgcaa atcgcatttg gcgagtttgt gccttggttg ctgcgctgcc ccgatttaac
     5581 cgtgcggcaa
//
LOCUS       DASTEY010000321         3949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_765067, whole genome shotgun sequence.
ACCESSION   DASTEY010000321 DASTEY010000000
VERSION     DASTEY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765067"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="lpxK"
                     /locus_tag="VFO72_05690"
     CDS             <1..610
                     /gene="lpxK"
                     /locus_tag="VFO72_05690"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET9478813.1"
                     /translation="ESFGTECFILDDGFQHLRLARNLDIVTIDATNPWGGGHLLPYGR
                     LREPISGLRRADCIVLTRCDQTQTDNLDALKDQIRAFIRGRPLFESMMSAPSKSNSPG
                     PVAAFCAVGNPESFFSQLKKAGYEVVVTKSFTDHHRYTQRDIDELASKATRAGAKALI
                     TTAKDAVKLRSLSIPLPYQVFEIEIAIKDEELFLNLLRETLA"
     gene            complement(582..2198)
                     /locus_tag="VFO72_05695"
     CDS             complement(582..2198)
                     /locus_tag="VFO72_05695"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9478814.1"
                     /translation="MKTCPKCGVEYPDTTTLCPADGVALETTSDSLIGTTLAGKYRID
                     ARLNEGGMGTVYRATHVLMEKTVAIKVLRPSLAADEKIVARFSREARAASRISHPNAL
                     SVTDFGEDENGIVFLVMEFLSGKTLKQVIRDEGPLPLQKVVEITRQVGDALNAAHLQG
                     VVHRDLKSDNIMLLDSTAGEHAKVLDFGIAKINEPGEARDAGLTAPNLVIGTPQYMSP
                     EQCSQETEIDSRSDIYSLGVILYEMLVGHVPFTADSPTMVMMKHLQEPVPSVLEERPD
                     LPAAIGRVIARAMAKLPANRYQTIAELVEDLTIASGLAQFAPVPAQPANVGASATAVT
                     DEPDEVTVVRTRQEPPPPVLRRAPVTVPVQGATSGPMPPVAAAASFNPLKVIIPSVIG
                     LLVVFVAIYAWKASSVANEPNANQPQQELAADPNSQPVQPGASPTGKSEEGIPVGGSV
                     VPPANVSASPNVNVAVSPEPIEDVSPVANANANENSNSNSNSNSNRKTPPLPEPTRSV
                     MPPEGVPSPAATKPPERASPVPTPTSPGGD"
     gene            complement(2391..2726)
                     /locus_tag="VFO72_05700"
     CDS             complement(2391..2726)
                     /locus_tag="VFO72_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET9478815.1"
                     /translation="MSKFRILLFAVVGILMSAMTVFAQAEAATGAADNASSVSKYRAL
                     AAGIGFGIAVIGGALGQSRIGAAACEGAARNPGAAGRIQTMMILGLALIESLVLFALL
                     VIFAVVKPS"
     gene            complement(2968..3903)
                     /gene="atpB"
                     /locus_tag="VFO72_05705"
     CDS             complement(2968..3903)
                     /gene="atpB"
                     /locus_tag="VFO72_05705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9478816.1"
                     /translation="MFWLQEAAHGAAEHAEETAEHAEEAVHHAPIIVQLVNHWFGEAA
                     LEFQLKYTKPIWDKVLGWFGTTPEATFGPYTAENAIPWYTVMFFIACILSVLIIWILK
                     GKLSESAPGHGQQTLEVSVLAVRSMIQDIIGPHGLKYFPVVMTCAVLILVSNLMGLCP
                     LFMSPTAATSVTFALGLSSFLYYNYIGISENGIIGHLKHLAGPIWWIAWLIFPIELIS
                     NLIRPFSLGIRLFGNLFADEKVLETVVNLAPPYSQWLVPLPLMALSVFVALVQTFVFI
                     LLSQLYLSEVSHAPHDHAHDAEHYEGDELIAPVLT"
BASE COUNT          841 a         1199 c         1058 g          851 t
ORIGIN      
        1 ggagtctttc ggcactgagt gtttcatcct cgacgacggt tttcaacacc tgcgcctcgc
       61 ccgcaacctc gacatcgtta ccatcgacgc aacgaatccg tggggcggcg gacaccttct
      121 tccctacggt cgtttgcgcg agccgatctc cggtctgcgc cgcgccgact gcattgtcct
      181 cacgcgctgc gatcaaacac aaacagacaa cctcgatgcc ctcaaagacc aaatccgtgc
      241 ttttatccgt ggccgtcctc tcttcgagtc gatgatgagc gcgcccagca agtcaaactc
      301 ccccggcccg gtcgccgcat tttgtgccgt cggaaacccg gagtcgttct ttagtcagtt
      361 aaaaaaggcg ggctatgaag tcgttgtcac gaagagcttc acggatcatc atagatacac
      421 gcaaagagac atcgacgagc ttgcgagcaa agcaacacgc gcgggcgcca aggccctgat
      481 caccacggcc aaagacgccg taaagctacg ctcactctct atcccactgc cttatcaggt
      541 ttttgaaatt gagatcgcaa ttaaggacga agagcttttc cttaatctcc tccgggagac
      601 gttggcgtag gaaccgggct cgctcgctcg ggcggtttgg tggccgccgg tgacggcacg
      661 ccttcgggcg gcataacgct gcgcgtgggc tcaggcaacg gcggcgtctt tcgattactg
      721 ttgctgttac tattgctgtt gctgttctca ttcgcattcg cattcgccac cggactcacg
      781 tcctcgatcg gctccggcga taccgcaaca ttcacattcg gactcgcgct cacattcgcc
      841 ggcggaacga cgctcccgcc taccggaatt ccttcttcac tctttccggt gggtgatgct
      901 cctggctgca ccggctgact attcggatcg gcggcgagtt cctgctgcgg ctggttggca
      961 ttcggttcat tcgcgactga gctggccttc cacgcataaa tcgcaacgaa caccacgagc
     1021 aacccaatca ctgagggaat aattaccttc aacggattga aactcgcagc agcagcgact
     1081 ggcggcattg gaccagatgt cgcaccttgc acgggtactg tcaccggcgc gcgccgcagc
     1141 acgggcggtg gtggttcttg tctcgtcctc accaccgtca cctcgtcggg ctcgtcagtc
     1201 acggctgtcg cggaagctcc aacattcgcc ggttgagcgg gcacgggtgc aaactgcgcc
     1261 agtcctgatg caatcgtcag atcttcgacc agttccgcga tcgtctggta acggttggcg
     1321 ggaagtttcg ccatcgcgcg ggcgatgact ctgccgatcg ccgcgggcaa gtcaggtcgt
     1381 tcctcgagca ccgacggcac cggctcctga aggtgcttca tcatcaccat cgtcggcgag
     1441 tcggctgtga acggcacgtg tccgaccaac atttcgtaca gaatcacgcc gagcgaatag
     1501 atgtcagacc gcgagtcgat ctctgtttcc tgcgaacact gttcgggcga catgtattgc
     1561 ggcgtgccga tgacgagatt cggggccgtg agcccggcgt cgcgagcctc cccgggttcg
     1621 ttaatcttcg caataccaaa gtcgagcacc ttcgcgtgct cgcccgcggt ggagtcgagc
     1681 agcatgatgt tgtcgctctt cagatcgcga tgaactacgc cctggaggtg cgcggcgttg
     1741 agtgcgtcgc caacctggcg cgtaatctcc acgacttttt gtagtggcaa cggtccttcg
     1801 tcgcgtatca cctgcttcag cgtcttgcca ctcaaaaact ccatcacgag aaacacgatc
     1861 ccgttctcgt cctcgccaaa gtcagtaacg ctgagtgcat tcggatgtga gatacgcgaa
     1921 gccgctcgcg cttcgcgcga gaaacgcgcc acgatctttt catcggcagc aagggacggc
     1981 cgcaacactt tgatcgccac tgtcttctcc atcaggacgt gcgtggcgcg atagacagtt
     2041 cccatgccgc cttcattgag acgtgcgtca atacgatatt ttccggcgag agtcgtgccg
     2101 atgagagaat cgctggtcgt ttcgagcgca acgccgtcgg ccgggcagag cgtagtcgtg
     2161 tcgggatatt caactccgca cttcggacat gttttcatca gtcgtcagcc tatcacacta
     2221 ccctgaagaa cttcgacagg attaagaaga cttacaagat actgtaagca attggcatca
     2281 cgcgctttgt tcctaaccat cctgtgactc cttttaatcc tgtccagcct acagttttag
     2341 gggcggccac aaagtgccgc cccgtacagg ctacttggat gtaagtgaga ctagctgggt
     2401 ttgacgacgg caaagataac cagcagcgca aacaacacca gcgattcgat cagcgccaga
     2461 ccaagaatca tcatcgtctg gatacgaccg gcagctccgg ggttgcgggc cgcgccttcg
     2521 cacgccgccg cgccaatgcg cgactgtccg agcgcaccgc cgattacggc gatcccgaac
     2581 ccgatacccg ccgccagtgc gcgatacttg ctcactgaac tcgcattgtc agcggcgcct
     2641 gtggctgctt cagcctgtgc aaacacggtc atggcgctca taagaatgcc gaccaccgcg
     2701 aacagcaaga tcctgaattt gctcatgcgt cctcctcata aatgaatccg attgcagcct
     2761 atgagcagcc ccgacgctat tatttcgcct gcatccggta tccaccgccc ctcttacaac
     2821 ggcgctccag cgagacagac actacccttg ttatgaattg tgccggtact actctgactt
     2881 cggttagggc aatctaaact acgaggtaac gtgtgcagcc gcaccggctg catcgctcac
     2941 cacgcgctac tcgataaagc caatgtatta agttaggact ggagcaataa gctcgtcgcc
     3001 ctcgtagtgt tccgcatcat gcgcatgatc atgcggcgca tgcgaaacct cgctcaggta
     3061 caactgcgag agcagtatga aaacaaatgt ctggaccaac gccacgaata cactcaaagc
     3121 catcagcggc agcggcacga gccactgcga atacggtggc gccaggttca ccacggtctc
     3181 cagcactttt tcgtcggcaa acaggttgcc gaatagacga atgccgagtg agaacgggcg
     3241 aatcaggtta ctaatcaact cgatggggaa gatcaaccac gcgatccacc agattggacc
     3301 agcaaggtgt ttcaagtggc cgattatgcc gttctcgctg ataccgatgt agttgtaata
     3361 aagaaacgag ctcaggccga gcgcaaacgt gacgctcgtg gctgccgtcg gactcatgaa
     3421 cagcggacag agtcccatca ggtttgagac gagaatcagt acggcgcagg tcatcaccac
     3481 cgggaagtac ttcaatccat gtggtccgat gatgtcctgg atcatgctgc gcaccgcgag
     3541 cacgctgacc tcgagcgtct gttgcccgtg gcccggtgct gactcggaaa gcttgccttt
     3601 gaggatccag atgatcaaaa cgctgagaat acacgcgatg aaaaacatca ccgtgtacca
     3661 cggaatggca ttctcggccg tgtatggtcc aaaggtcgcc tcgggcgtgg tcccgaacca
     3721 acccagcact ttgtcccaaa tcggtttggt gtatttgagt tggaattcca gcgcggcttc
     3781 gccaaaccag tggtttacaa gctgcacgat gatgggcgcg tggtgtacgg cttcctcggc
     3841 gtgctctgcc gtttcctccg cgtgctctgc cgccccgtgt gcggcttcct gcaaccagaa
     3901 catcaactaa tttcctctcg ctcgataatt gcgaaataga actctcttg
//
LOCUS       DASTEY010000322         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_56378, whole genome shotgun sequence.
ACCESSION   DASTEY010000322 DASTEY010000000
VERSION     DASTEY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56378"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4543
                     /locus_tag="VFO72_05710"
     CDS             <1..>4543
                     /locus_tag="VFO72_05710"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET9478817.1"
                     /translation="IAACRELLNHDPGNELPAWFVLFDALERRTSDIDDIVNALAHEH
                     GEINFKELRWWVGALRNQVICAQRDAESVTTWGRLLLQLTQAVGANEEWAAVIKLLQE
                     VPTPAEIPQLCDNALVQLSAFQDHDSPQLAQLSAKLTTALEQAASGSNDLLSRLSRIA
                     RTCDQIVEDMDFSFLFDVERKLFTIGYNATASRADDSYYDLLASEARLASFVGIAKGD
                     VPQQHWFRMGRALTKVDGGRALLSWTGTMFEYLMPLLVMRNYPATLLAETYETIVERQ
                     IEYGQERGVPWGVSEAAYNVRDLQLNYQYGPFGVPGLGLKRGLVEDLVVAPYATMLAA
                     PVDSQAAMNNLRRLQNEGALGAFGFYESIDYTAARLPEGQKSVLIRAFMTHHQGMSLV
                     ALTNLLQDYVMERRFHSDPSVQATELLLQERIPIGVPAAHPRAEEVLTGRVIQIMPGM
                     ITRVYESADLDTPRTQLLSNGTYNVMLTTAGSGYSRCEENAVTRWREDVTRDNWGFYI
                     YLRDVRSGTVWSAGHQPVRRRPQAYTVAFSEDKADFRRIDSGISTRLEVVVSAEDNAE
                     VRRVSLTNHSSRTREIELTSYGEVVLNTPATDAAHQAFSNLFIETEFIPNENALLAHR
                     RQRSAEDRPIWGIHVVVVEGDSVGAIQYETDRGRFLGRGRTASNPIAVMEDRPLSNTT
                     GAVLDPVFSLRRRVRIQPNQTVSCSFSLAVAHSREDALALADKYHAPNIFERELRLAW
                     TKAQVEMSHLKIDAEEAHLFQRLAARIVFSDPSLRPTPHVLALNTKSQSSLWAHGISG
                     DLPIIVVRINKAADIPSIKKMVRGHEYLHYKGLRSDLVILNDTPTSYLEPLHKELETI
                     VRTSGLQGLQDKPGGVYLRRADQMPEAERILLHAVARVVVVTERGRFEDQIERKRVEE
                     PLPPMLMPRQASQTYPEPTMAPPELSFFNGLGGFHQGGREYVTLLGAEQWTPAPWSNV
                     IGNTVEFGFQITETGGGYTWSTNSRENRLTPWSNDAVSDPPGEVVYLRDEDSGTVWSA
                     TPLPIREREPYIIRHGQGYTVFEHISHGLSQELLLFAPLDASVKISLLRLRNRTGRKR
                     RLSITNYNELVMGVARSSSAPYIITEIDEPAASIFARNPYNNEFAQLITFVATNETLA
                     SATCDRKEFIGRNGSLDAPAALRRVSLAGRDGAGLDPCAAIQTSIELAPHEGREVIFL
                     LGQAESKEAAQSLIATFRQPANVNAAFEKVLNHWDELLGTVEVRTPDQALDTMLNRWL
                     LYQTLSCRVWARTAFYQSGGAFGFRDQLQDVMALVYSRPEITRKQIMLAAAHQFKEGD
                     VQHWWHPPSGRGVRTTISDDLLWLPFVTAFYIRVTGDTSVLDEVVPFVEQPLLEPGEL
                     ESYRQPEISSESASIFEHCARALDRSLKVGEHGLPLMGGGDWNDGMNRVGWLGKGESV
                     WLGWFLYNALAMFTPQVEARKEKERAKRYRAHLDHLKKALEEKGWDGDWYRRAYFDDG
                     TPLGSARNEECRIDSIAQS"
BASE COUNT          979 a         1369 c         1311 g          884 t
ORIGIN      
        1 agattgccgc ctgccgcgag ttgctgaacc acgatcccgg caacgagctg cccgcatggt
       61 tcgtgttgtt cgatgcactg gagcgccgca cttccgacat cgacgatatc gtcaacgcgc
      121 tggcccacga gcacggcgaa atcaatttca aagagttgcg ctggtgggtc ggtgctctac
      181 gtaatcaggt aatttgcgct cagcgagacg ctgagtcggt cacgacgtgg ggccgccttc
      241 tgctccagct aacgcaagct gttggcgcaa acgaagagtg ggccgctgtc atcaagctgc
      301 ttcaggaagt tcctacaccg gccgagattc cgcaactctg tgacaacgcg ctggtccaac
      361 tctccgcttt tcaggatcac gactcaccgc aactcgccca gctctcggcc aaattgacga
      421 cagcactcga gcaagccgcc agcggttcaa acgatctgct gtcgcgtctg agccgcatcg
      481 cgcgaacctg cgaccagatc gtcgaggata tggacttctc gttcctcttc gacgtcgaac
      541 gaaaactgtt cacgatcggc tacaacgcta cggcgtcacg cgccgacgac tcttactacg
      601 acctgctggc cagcgaagcg cgccttgcga gttttgttgg catcgccaaa ggcgacgtgc
      661 cgcaacaaca ctggttccga atgggacgcg cgttgacgaa agtcgatggt ggaagagcgc
      721 tgctttcgtg gaccggcacc atgtttgagt acctgatgcc gctgctcgtg atgcgcaatt
      781 accccgcgac gctgctcgcc gaaacatacg agacaatcgt cgaacgtcag atcgagtacg
      841 gccaggaacg cggcgtgccg tggggagtgt cggaagcggc ctacaacgtt cgtgaccttc
      901 agctcaatta ccagtatgga ccattcggcg tccccggtct cggtttgaaa cgaggactcg
      961 tcgaagacct tgtagtcgcg ccttacgcaa cgatgcttgc cgctcccgtc gattcacagg
     1021 cggccatgaa caatttgcgc cggctgcaaa atgagggagc tttgggagcg ttcgggtttt
     1081 acgagtcgat cgactacacg gcggcacgcc tgcccgaagg tcagaagtcg gtactgattc
     1141 gcgctttcat gactcaccac cagggcatga gtctcgtcgc gttgacaaat ctccttcaag
     1201 actacgtgat ggaacgtcgc tttcacagcg atccgtccgt gcaggcgacc gagttgctgt
     1261 tgcaggagcg aatcccgata ggtgtcccgg ccgcccatcc gcgcgcggaa gaagtgctga
     1321 ccggacgcgt aatccaaatc atgccgggca tgatcacgcg cgtctacgaa tcggcagatc
     1381 tcgacactcc acgtacgcaa ctactctcaa acggcactta caacgtgatg ctcacgaccg
     1441 ccggctccgg ttattcacga tgcgaggaga atgccgtaac acgctggcgc gaggatgtca
     1501 cgcgcgataa ctggggcttc tacatttacc tgcgcgacgt gcgcagcggc accgtctggt
     1561 ccgccggtca ccaaccggtg aggcgcagac cgcaggcata cacggtcgca ttttccgaag
     1621 acaaagccga ctttcggcgc atcgactccg gcatctcgac ccgcttggaa gtcgtggttt
     1681 ccgccgaaga caacgcggag gtgcggcgcg tgtcgctgac taatcactcg tcgcgaactc
     1741 gcgagatcga attaacgagt tacggcgaag tcgtgctcaa cacgcccgca accgacgcgg
     1801 cacaccaggc gttcagcaat ctctttatcg aaactgaatt cataccaaac gagaacgcgc
     1861 ttttggccca tcggcggcag cgctcggcgg aagaccgtcc gatttggggg attcacgtcg
     1921 tcgtcgtgga aggcgactca gtcggcgcaa ttcaatacga aaccgatcgc ggacgatttc
     1981 ttggacgagg gcgcaccgcg agcaatccga tcgccgtcat ggaagatcgc ccgctttcaa
     2041 acaccaccgg cgcagtgctc gatccggtgt tcagcttgcg acgccgtgtg agaattcaac
     2101 caaaccaaac agtaagctgc tcgttcagtc tcgcggtggc ccattcgcgc gaagatgcgc
     2161 tggcgctcgc cgacaagtat cacgcgccaa acatcttcga acgcgaactt cgtctcgcct
     2221 ggaccaaagc gcaggtcgag atgagccact tgaagatcga cgctgaagaa gcacacctgt
     2281 ttcaacgtct ggcagcgaga atcgtgttct ccgatccttc gctgcggccc acgccgcacg
     2341 tactggcgct gaatacgaag tcgcagtcga gtctttgggc ccacggtatc agcggcgacc
     2401 tgccgatcat cgtcgtccgc atcaataagg ccgccgatat tccgtccata aagaaaatgg
     2461 tccgtggtca tgagtacctg cattacaaag gactgcgcag tgacctggta attctcaacg
     2521 acacgccgac gagttatctc gaaccgctcc acaaagagtt ggaaacgatc gtgcgtacga
     2581 gcggtctgca ggggctccag gacaaaccgg gcggcgttta cctgcgtcgc gcggaccaga
     2641 tgcccgaggc cgaacgcatc ctgctgcacg ccgtcgcgcg cgtagttgtc gttaccgagc
     2701 gcggtaggtt cgaagatcag atcgaacgga agcgtgtcga ggaaccgcta ccgccgatgc
     2761 tgatgccgcg ccaggcttca caaacttatc ccgaaccaac gatggcgccg cccgaactca
     2821 gtttcttcaa cgggctcggc gggtttcatc agggcggacg cgagtacgtc acgctgctcg
     2881 gcgccgaaca atggacgccc gcgccgtggt ccaacgtcat cggcaacacg gtcgaattcg
     2941 gttttcagat caccgaaacg ggaggcggtt atacgtggtc gaccaatagc cgcgagaatc
     3001 gtttgacgcc gtggtccaat gacgcggtca gcgatccgcc gggtgaagtc gtttacctgc
     3061 gcgacgagga ttccggcacc gtctggtccg cgacgccgtt gccaattcgt gaacgcgagc
     3121 catacatcat caggcacgga cagggctaca ccgtgttcga acacatcagc cacggcctct
     3181 cgcaggagtt gttgctcttc gcgccgttgg atgcttcagt caagatctcc ctgctgcgtt
     3241 tgcgaaatcg cacgggccgg aagagacgat taagtatcac taactataac gaactggtga
     3301 tgggcgtcgc gcgctcgtcg tcagcaccgt acattattac ggagatcgat gagcccgccg
     3361 cgtcgatctt tgcgcgcaat ccctacaaca acgagttcgc tcagttgatt acttttgtgg
     3421 cgacgaacga aacgctcgcc agcgcgacct gcgatcgaaa ggagtttatc ggacgcaatg
     3481 gttcgctcga tgcgcctgcc gcgctacggc gtgtgagtct cgccggacgc gatggtgccg
     3541 ggctcgatcc gtgcgctgcg attcaaacca gtatcgaact ggctccgcac gaaggtcgcg
     3601 aagtgatctt tctgctgggc caggccgaat cgaaagaagc agcacaaagc ctgatcgcaa
     3661 ctttccgtca gccggcaaac gtcaatgccg ctttcgaaaa ggtattgaac cactgggacg
     3721 aactgctcgg caccgtggaa gtgcgcacgc ccgatcaggc gctggacacg atgctcaacc
     3781 gctggctgct ttaccagacc ctatcgtgcc gcgtctgggc gcgcactgct ttctaccaat
     3841 ccggcggtgc cttcggcttt cgggatcagt tgcaggacgt gatggcgctg gtctacagcc
     3901 ggccggagat tacgcgaaaa cagatcatgc tggccgccgc acaccaattc aaagaaggcg
     3961 atgtccaaca ctggtggcat ccgccaagcg gacgcggcgt gcgcacgacc atttccgacg
     4021 acctgctttg gcttcccttt gtcacggcct tttatataag ggtaactggc gacacgtctg
     4081 tcctggacga agttgttccc ttcgtcgaac agcccttact agagcccggt gagctcgaga
     4141 gttaccggca accggagatc tcctccgagt cagcatcgat ctttgaacac tgcgcccgag
     4201 cactggatcg cagtctgaag gtaggcgaac acggattgcc gctgatgggc ggcggcgact
     4261 ggaacgacgg tatgaaccgc gtcggctggc tcggcaaagg cgaaagcgtg tggcttggtt
     4321 ggttcctcta caacgccctc gcgatgttca cgccacaggt cgaagcacgg aaagaaaagg
     4381 aacgggcgaa acgctatcgc gcacacctgg accatctcaa gaaagcactt gaagaaaagg
     4441 gctgggacgg cgactggtat cgccgtgcct atttcgacga cggaacaccc cttggttcag
     4501 ctcgcaacga agagtgccgc atcgattcga ttgcgcagtc gtg
//
LOCUS       DASTEY010000323         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1556061, whole genome shotgun sequence.
ACCESSION   DASTEY010000323 DASTEY010000000
VERSION     DASTEY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556061"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..2219
                     /locus_tag="VFO72_05715"
     CDS             309..2219
                     /locus_tag="VFO72_05715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9478818.1"
                     /translation="MVQVSLRETLAEWWMTNQLRVLWVLLIVPFAIELVYGSFTLLYL
                     VLVPVSILATYLIYRSAKTRLQAKTREIEALGHLHLATAEALATAIDAKDQTTHCHVR
                     RVQIYAAGMGEVFGLDGNEIAALKAGALLHDVGKLAVPPHILNKPGPLTPAEFEKMKI
                     HTVVGAQILARVNFPYPVIPIVRHHHEQWDGRGYPDKLRGEQIPITARIISVVDCFDS
                     VREDRPFRRGMTLGEATALLLRGAGVHFDPVVVEQFIKHLPRFEEQIEALGLQHQPAN
                     YSAEPLQLSNIDLDQTRERGSYIAYDQIKKAHREVYALYEIARTFGTSLNVEHTLEIL
                     VDKVGHVVPFDTCIVYIYDDTKGYATARHVVGKNVQKLGSRSIALGEGVTGFALANRS
                     PVNQLHPSLDFTDINPEAGIRYRSMASLPLFKDEVLLGALSVYSSELEQYTDDHMRLL
                     ETVTRLASDALGNAMQHALAESNALTDQLTGLPNARYLSLRFDEEAARARRTDRAFQV
                     MMLDLDQFKSVNDTFGHKAGDKMLREVAHIIEGQLREYDFLARYGGDEFVALVQEVVG
                     SQVDELCLRIENAVSKFSLMVGRNRYARVGISIGTATFGIDGETLDHLVVAADEKMYR
                     MKSSHRLERTVA"
     gene            2266..>3661
                     /locus_tag="VFO72_05720"
     CDS             2266..>3661
                     /locus_tag="VFO72_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9478819.1"
                     /translation="MVSPAENQNATLLIVDDDHAARERLREIFETAQYRVVTANEAAS
                     ALRVLRGVRCDLVALDLEMPGVDGLALCKLLRAQPTTSKLPIIALSESDDEARKVQAF
                     TAGADDYITKPSTPGELLSRVSLHVRTSQREWALIGSNRELRFLSDIGRGLLRALEPE
                     QLVRRVAGATYDALDASLCATYVITGRDKHAVCVFDREGSADDHSLLKQEALQEWLRS
                     ADSASSLLITHPSDFYLSDDIHTIEYLAPFRFAGRAKGALIVAFDLREQCGETERRLI
                     DAAAQQTSLAAHICSLYQAARDTSVSLAREVERRTAESEAQKRFIEAIVDSLPLSLYA
                     IDREYRVAAWNRNRELGELGIPRGSVLGKNVFEVLTRQPREMLEEEFARVFESGEIER
                     IEQETPSPKGDLRHWVISKIPMWADTTGQVSHVITVGEDVTDRVEANRAVARAEKLAA
                     VGRLAAGVVHEINNP"
BASE COUNT          804 a         1064 c         1029 g          764 t
ORIGIN      
        1 caagctttga cattggcgat ctttcgattg tcgattgccg attgcgccca gatattgtcg
       61 attgcggtag tcaaatcgaa aatcggcaat agaaaattga caatgtaagt atctcaccca
      121 gcacggcagt cctgttccag accaggactg ccacttgccc aatttgccgg ggacggagtt
      181 gagttgctcc cagctttgtc tccggcacca gactgcgtga gagattgatt tgagtgcaga
      241 cgaacccgat cgcgtttcca ggagaatcaa tgttccttcc accacggttt gaatttaacc
      301 gccatccggt ggtgcaagtt tcccttcgtg aaactctggc cgagtggtgg atgacaaacc
      361 agctccgggt gctttgggtt ctactgattg ttccgttcgc aattgaactt gtttacggct
      421 cgtttactct gctctatctc gttctcgttc ccgtttcaat actcgcaact tatctgatct
      481 atcgcagcgc gaagacacgc ctgcaagcaa agacgcgtga gatcgaagct ctggggcacc
      541 tgcatctcgc aaccgccgag gcattggcca ccgccatcga cgcgaaagat caaacaacgc
      601 attgtcacgt gcgtcgcgtg cagatctacg ccgccggcat gggcgaagtg tttggcctcg
      661 atggaaacga gatagctgca ctcaaagccg gcgcgctact ccatgatgta ggtaaactcg
      721 ctgtcccgcc ccatatcttg aacaagccag gacccttgac gccggccgag tttgaaaaga
      781 tgaagatcca cacggtcgta ggagcccaga tcctcgcccg tgtgaatttt ccttatccgg
      841 tgattccgat cgttcgccac caccacgagc agtgggatgg tcgcggttat cccgacaagc
      901 tgcgtggtga acagatcccg attaccgcgc gcatcatttc tgtcgtggac tgtttcgact
      961 cggtgcgtga agaccgtccg ttccggcgcg gcatgacgct gggcgaagcg accgcgttgt
     1021 tattgcgcgg cgcgggcgtt cacttcgatc cagtggtggt cgaacagttc atcaaacacc
     1081 taccgcgctt cgaggaacag atcgaagccc tcggattgca acatcagccc gcgaactatt
     1141 cagcggaacc acttcagttg agcaacatcg atctggacca gacgcgcgaa cgcggctctt
     1201 acatcgctta cgaccagatc aagaaagccc atcgcgaagt ttatgcgttg tacgagatcg
     1261 cgcgtacgtt tggtacgtcg ttgaacgtcg aacacacgct cgagattctc gtcgacaaag
     1321 ttggtcacgt tgttccgttc gacacgtgca tcgtctacat ctacgacgac acgaagggct
     1381 acgccactgc gcgtcacgtg gtcggcaaga acgtgcagaa gctcggctcg agaagtattg
     1441 cactgggcga aggcgtaacg ggatttgccc tggcgaaccg tagcccggtg aaccagctcc
     1501 atcccagtct tgacttcacc gatatcaatc ccgaagccgg catcaggtat cgttcgatgg
     1561 cttcgttgcc gctgttcaaa gatgaagttt tactcggcgc attgtccgtc tactcgagcg
     1621 agctcgaaca atacacggac gatcacatgc gtttgttgga gacagtaacg cggcttgcct
     1681 cggacgcact cggcaacgcg atgcaacacg cgttggcgga atcgaatgcc ttaaccgatc
     1741 aactcaccgg tttgccaaac gcccgttacc tgtcgctgcg gttcgatgag gaagcagccc
     1801 gcgcgcgccg taccgatcgc gcgtttcagg tgatgatgct cgaccttgac caattcaaga
     1861 gcgtcaacga cacgtttggt cacaaagccg gtgacaagat gttgcgcgaa gtggcccaca
     1921 tcatcgaggg acagttgcgc gagtacgact ttctggcgcg ttacggtggc gatgagtttg
     1981 tcgccctcgt tcaggaggtc gtcggctcgc aagtcgatga gttgtgcctg cgaattgaaa
     2041 atgcggtgtc gaagttctcg ctgatggtcg gccgcaaccg ttacgcacgc gtcggcatca
     2101 gcataggcac cgccacgttc ggaatcgacg gcgaaacgct ggaccacctc gtagtggccg
     2161 ccgacgagaa gatgtaccgc atgaagtcga gccacagact ggaacggacc gtggcttaga
     2221 aatccttgca gccacagttt caatctgtgt aatctgtggc aaggaatggt atcgcccgcc
     2281 gaaaaccaaa acgccactct cttaatagtc gatgacgatc acgccgcccg tgaacgactg
     2341 cgcgagatct tcgaaaccgc gcaataccgt gtcgtcacag ccaacgaggc ggcttccgcc
     2401 ctgcgcgttc tgagaggcgt gcgttgcgac ctcgtcgccc tcgatctcga gatgcccggc
     2461 gtcgacggtc tcgcactctg caaactactt cgcgcccagc cgacaaccag caaactgcca
     2521 atcatcgcgc tctctgaaag tgacgatgaa gcgcgcaaag tgcaggcctt caccgccggc
     2581 gccgacgact acataacaaa gccgtccacg cccggcgagt tgctctcgcg cgtcagcctg
     2641 cacgttcgca cgtcgcaacg cgaatgggcg ctcatcggca gcaaccgcga actgcggttc
     2701 ttgtcagaca tcggtcgggg actgctgcgc gcgctcgagc cggaacaact tgtgcgacgt
     2761 gtcgcaggcg caacctacga cgcgctcgac gcttcgcttt gcgcgacgta tgtgattacc
     2821 ggtcgagaca aacacgccgt ctgcgttttc gatcgcgaag gcagtgcaga cgatcactca
     2881 ctgctgaaac aggaagcact acaggagtgg ctgcgttctg ccgactcagc gagttcgttg
     2941 ctgatcacgc atccatccga tttttatctc agcgacgaca ttcacacgat cgagtacctg
     3001 gctccctttc ggttcgccgg cagagcgaaa ggcgcactaa tcgttgcgtt cgatctccgt
     3061 gaacaatgcg gcgagactga acggcgtttg atcgacgcag cggcacaaca gacgtcactc
     3121 gcggcccaca tctgttcgct ttaccaggct gcgcgcgaca catcggtgag cctcgccaga
     3181 gaagtcgaac ggcgcacggc cgaaagcgaa gcacaaaaac gattcatcga agctatcgtc
     3241 gatagcctgc cactgtctct gtacgcaatc gatcgcgagt atcgcgtcgc ggcctggaat
     3301 cgcaatcgcg aactcggtga gctcggtatc ccgcgcggtt cagtactcgg gaagaacgtc
     3361 ttcgaggtgc tcactcgcca accgcgcgaa atgctggaag aggagttcgc gcgcgtgttc
     3421 gaaagtggcg agatcgaacg tatcgagcag gagacgccga gcccgaaggg cgacctccgg
     3481 cattgggtga ttagcaagat tccgatgtgg gctgacacga cgggacaggt gagtcatgtt
     3541 attactgtcg gtgaagacgt taccgaccgt gttgaagcaa atcgagcagt ggcgcgtgcg
     3601 gagaaacttg ctgccgtcgg tcgactggct gcgggtgtgg tccacgagat caacaacccg
     3661 c
//
LOCUS       DASTEY010000324         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_435467, whole genome shotgun sequence.
ACCESSION   DASTEY010000324 DASTEY010000000
VERSION     DASTEY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435467"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1297)
                     /locus_tag="VFO72_05725"
     CDS             complement(203..1297)
                     /locus_tag="VFO72_05725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478820.1"
                     /translation="MNIAFFGSSLVSAYWNGAATYYRGIIRALHRLGHRVTFYEPDAY
                     DRQKHRDIPDPDWARVVVYPVDNEQGVLRALEQARGVDLLIKTSGVGVFDRFLESAVL
                     ELKAPHTLSVFWDVDAPATLDRVNTDKSDPFKDLVPLYDLILTYGGGEPVIRRYKAFG
                     ARACVPIYNALDPDTHHPVPLDERFACDLALLANRLPDREARIEEFFFDVARGLPNHK
                     FVLGGNGWADKSMPANVKYVGHVYTRDHNAFNCSARTVLNVNRDSMARYGFSPPTRIF
                     EAAGSATCLITDTWEGIETFLEPDREVLVARGSDDVVRQLRTLGQSRARQIGEAALQR
                     VLAEHTYQHRAEQLQQLLDGGMFERVEDAA"
     gene            complement(1294..2406)
                     /locus_tag="VFO72_05730"
     CDS             complement(1294..2406)
                     /locus_tag="VFO72_05730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478821.1"
                     /translation="MRIAFFYQSLISDWNHGNAHFLRGVTSELLARGHNVDVYEPRNS
                     WSVQNLIAEQGWTPLIEFERAYPQLASIRYDLGQLDLDEALNSADLVIVHEWNESELI
                     ERIGEYRRTRDNLVLLFHDTHHRIAGHEDLPFSLKHYDGVLAYGQVLTDRYLRNGWTE
                     RAWTWHEAADTRVFHPLPNVARTLDLVWIGNWGDDERTAELHEFLIEPVRDLKLHAEV
                     YGVRYPDSAMAALAQAGIRYRGWLPNYRVPEVFAASRLTLHIPRRPYVRELPGIPTIR
                     PFEALACGTPLICSPWNDVEHLFTPGRDFLVAENGEEMKRLINKVLSEPAFADELSTH
                     GPATIRQRHTCAHRVDELLEIYSQVLTKTKQLVAAL"
     gene            complement(2397..3503)
                     /locus_tag="VFO72_05735"
     CDS             complement(2397..3503)
                     /locus_tag="VFO72_05735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9478822.1"
                     /translation="MTADTVGGVWTYALELTRALEPHGVEVQLALMGPPLTTAQREEA
                     DEIANLSLFKSDYKLEWMPECWPDVRRAGEWLLHLANRLNPDLIHLNGYAHANLPWTS
                     PVLVVGHSCVFSWWQAVRGDTPPAEWQRYKSEVTNALRGAHFVVAPTVAMLRALETHY
                     GKIYNGRVIANGRDKECFKPAEKQRFILSAGRLWDRAKNIERVAELAPELPWPVFVAG
                     ECRTPERPEQTRAFNDKCNSVGQLSESELRNWFGAASIYALPALYEPFGFTPLEAGLS
                     ACALVLGDIESLREIWDGAAVFVDPTDGGALKSALLQLISNDNYRHEMAHRARVRALQ
                     FSTRRMAEDYLAAYADLLTLSESFRKQESLAQCA"
     gene            complement(3527..>3955)
                     /locus_tag="VFO72_05740"
     CDS             complement(3527..>3955)
                     /locus_tag="VFO72_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478823.1"
                     /translation="PITRTEATLFSAGHLLPKPIHQVEDYATATIETAGNTVIDLSCS
                     WNLHAGRPADIEITFYGTGGGASLQNIDGSFFDFVAKRFNGTETETLNTPADSQWRWG
                     GLATLEWVKKLAANERFDPSIERLMTVAEIIDAIYGRGPK"
BASE COUNT          854 a         1170 c         1099 g          832 t
ORIGIN      
        1 ccagcggttt caccatcgca tttcaaggcg cctctttcct gggaccgcag gcgtctcgcc
       61 tgcatggttt gggtcgggcg ctgccgtgac tagcaggcga gacgcctgcg ctcccagtat
      121 tcagggcccc aaagttccaa ggggtcccaa tgttccaggg ttctggaagt tccagacttc
      181 attgctttag tagccatcgt aactaagcag cgtcctcgac tcgttcaaac atgccgccgt
      241 cgagcagttg ctgaagctgt tccgcgcggt gctggtaagt atgttcagcc aacacacgtt
      301 gcaacgccgc ctctccgatc tgtcgcgcgc ggctctggcc cagtgtgcgc aattgacgaa
      361 caacatcgtc cgaaccgcgc gccacgagca cttcgcgatc cggctcgaga aacgtttcaa
      421 tcccttccca cgtgtccgtg attaaacacg tcgccgatcc ggcagcttcg aagatgcgcg
      481 taggcggcga aaacccatac ctcgccatgc tgtcgcggtt cacgttcaaa accgtgcgcg
      541 ccgaacaatt aaacgcattg tgatctcgag tgtaaacatg cccgacgtac ttaacatttg
      601 ccggcatcga cttgtcggcc cagccattgc cgccaagcac aaacttgtga ttgggcagtc
      661 ctcttgcgac gtcgaaaaag aactcctcga tacgcgcctc acgatccggc agtcgattcg
      721 ccagcagtgc caggtcacag gcaaaacgtt catcgagcgg gacgggatga tgcgtgtcgg
      781 gatcgagtgc gttgtagatc ggcacgcagg cccgcgcgcc gaacgctttg taacgacgga
      841 taacgggttc gccgccgcca tacgtaagaa tcagatcgta cagaggaacc agatccttaa
      901 acggatccga cttgtccgtg ttgacacgat cgagcgtcgc gggcgcgtca acgtcccaga
      961 acacggatag cgtgtggggg gcttttagtt ccagaacagc ggattccaaa aaccgatcga
     1021 agacaccaac gccactcgtc ttaatcaaca gatcgactcc acgagcctgc tccagtgcgc
     1081 gcagcacacc ttgctcgttg tcgaccggat agaccacgac acgcgcccaa tccggatcgg
     1141 gaatgtcgcg atgcttctgc cggtcgtaag cgtccggttc gtaaaacgtg acgcgatggc
     1201 ccaggcgatg cagcgcgcga atgatgcctc gatagtacgt ggccgcgcca ttccaataag
     1261 ccgagaccaa actggatcca aaaaacgcta tgttcataag gccgctacca attgcttcgt
     1321 cttcgtcaat acttgcgagt agatctccaa cagttcatca acgcggtgcg cacacgtgtg
     1381 gcgttgccgg atggtcgctg gtccatgcgt cgacagttcg tcagcaaacg ccggttcact
     1441 cagcaccttg ttgatgagcc gtttcatctc ctcaccattt tccgccacga gaaaatcgcg
     1501 cccgggcgtg aacaaatgtt ccacgtcgtt ccacggcgag cagatcagcg gagtgccgca
     1561 agcaagcgcc tcgaaaggac ggatggtggg aataccgggc agttcgcgaa cgtatggccg
     1621 gcgcggaatg tgcaacgtca aacgcgacgc tgcgaatacc tccggcacgc ggtagttcgg
     1681 caaccagccg cggtaacgaa tgcccgcctg tgcgagtgcc gccatcgccg aatcgggata
     1741 gcgaacgccg tacacttcag catgaagctt caaatctctg acaggttcga tcaggaactc
     1801 gtgcaactca gcagtacgct cgtcgtcgcc ccagtttcca atccaaacca ggtcaagcgt
     1861 gcgtgcgaca ttcggcagtg gatgaaacac gcgcgtatct gcggcttcgt gccacgtcca
     1921 cgcccgctcg gtccaaccgt tgcgcaggta ccggtctgtg agcacctggc cgtaagccag
     1981 cacgccgtcg tagtgcttca aactaaacgg cagatcttcg tgtccggcga tgcgatggtg
     2041 cgtgtcgtgg aagagtaaga cgagattgtc tctagtccgc cggtactcgc caatgcgttc
     2101 gatcaactca gactcgttcc actcgtgcac gatcacgagg tcggcgctgt tcaacgcttc
     2161 atcaaggtcg agttgcccga ggtcgtagcg aatactggcc agttgcggat acgcgcgctc
     2221 aaattcaatg agcggtgtcc aaccctgttc ggcgattaag ttttgaacgc tccacgagtt
     2281 gcgcggctcg taaacgtcaa cgttgtgacc acgcgccagt aactccgacg tgacaccgcg
     2341 gagaaaatgc gcgtttccgt ggttccaatc cgagatcaga gactgataga agaacgctat
     2401 gcgcactgcg ccaaactctc ctgtttgcga aaggattctg agagcgtcaa caggtctgca
     2461 taagctgcga ggtaatcttc agccatgcgc cgggtgctga attggagcgc ccgcacgcga
     2521 gcgcgatgcg ccatttcatg ccggtagttg tcgttactga ttagctggag tagcgctgat
     2581 ttcagcgcgc caccgtcagt cggatcgacg aaaacagccg cgccatccca tatttcgcgc
     2641 aacgattcga tatcgccaag caccagtgca cacgcagaca gtcccgcttc cagcggcgta
     2701 aaaccgaatg gttcgtagag cgcgggcagt gcatagatcg acgcggcgcc aaaccaattg
     2761 cgcagctcgg actctgagag ttgaccaacc gaattacatt tgtcgttgaa cgctcgcgtt
     2821 tgttcgggtc gttccggcgt gcggcattcg ccggcgacga aaacaggcca aggcaattca
     2881 ggcgcaagct cggcaacgcg ttcgatgttt tttgcccgat cccacagacg acctgccgac
     2941 aagatgaacc gctgtttttc agcgggcttg aaacactctt tatcgcggcc gtttgcgatg
     3001 acacgcccgt tgtagatctt gccgtagtga gtttccaaag cgcgcagcat cgcaacggtc
     3061 ggtgcaacaa caaagtgtgc gccacgcagt gcgttagtca cttcgctttt gtaacgttgc
     3121 cattcggccg gcggcgtatc gccacgcaca gcttgccacc aggaaaagac acatgagtga
     3181 ccgaccacga gcacgggact ggtccacggc aggttcgcat gcgcgtaacc gttgagatga
     3241 atcagatcgg ggttcagccg attggccagg tgcaacaacc actcgccggc gcgccgcacg
     3301 tcgggccagc actccggcat ccactcgagt ttgtagtcgc tcttgaacag actgagattc
     3361 gcaatctcat ctgcttcctc tcgctgtgcg gttgttagtg gcgggcccat caacgccaac
     3421 tgcacttcga cgccatgagg ttcaagtgcg cgcgtcaatt ccagcgcgta cgtccagacg
     3481 ccgcctacag tatcggcagt catgagaatc tttgtcggat acttcatcat tttggtccac
     3541 gtccgtaaat ggcgtcgatg atttccgcga ctgtcatcaa ccgttcaatc gagggatcga
     3601 accgttcgtt ggcggccagc ttcttgaccc attcaagggt ggccaggccg ccccaccgcc
     3661 attgcgaatc cgcgggtgtg tttagtgttt cggtttccgt accgttaaag cgttttgcga
     3721 cgaagtcgaa gaacgatccg tctatgtttt gtaacgaagc cccaccgccc gtgccgtaaa
     3781 aagtgatttc gatgtccgct ggccggcccg cgtgaaggtt ccaggaacaa ctaagatcga
     3841 tgaccgtgtt gccggcagtc tcgatcgtcg ctgtggcgta gtcttcaact tggtgaatag
     3901 gtttcggcag caagtgtccg gcggagaaca atgttgcttc agttcgcgtg atcgg
//
LOCUS       DASTEY010000325         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1539725, whole genome shotgun sequence.
ACCESSION   DASTEY010000325 DASTEY010000000
VERSION     DASTEY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539725"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1301)
                     /locus_tag="VFO72_05745"
     CDS             complement(108..1301)
                     /locus_tag="VFO72_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478824.1"
                     /translation="MRRITATISAATTIFLTLFLATGCKSVIERQDVRPKVLRDVPAR
                     NLAYRLSPDVDSPSTVEDPADKNGAVANHFSTGRKDDALVRTVASPDGRRVLALYGTP
                     DEPGSTFRIDLYNSEGQFLRNLIPPDIACVFPETVSWSPDGNFINFIARKRVMPSPTP
                     TPPNEPEPDPLAPPVPSPSVAPLFAPVASFNTEQIYICNRDGYDLKPLTSREGLIYFY
                     FVWAPDGHAMAALACKEDEWNARERQFKLPAGRPRLIAPSGSERLLDDALTDALPVWS
                     PDASKIATAFDTDVAIYDAVGKTPTQARMPLGDQLVAASLAYEQRTTTKKASTNADSP
                     APESTPVPGGVPPSFNPIVRLDWRAPETLYVQTAFVRVMPSETINTFQRWHTLTISAQ
                     AAILK"
     gene            complement(1309..2151)
                     /locus_tag="VFO72_05750"
     CDS             complement(1309..2151)
                     /locus_tag="VFO72_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478825.1"
                     /translation="MLLILASLPNAFSQDRPESHSTRSRPTMSAEAKELLEDAIGVVC
                     TQAKLDPKSSVAIDEMQARPSLPVHSPEARSGAERAQRLLPLAKTLVITALRQLSTEY
                     GFKRSPRFKVRINQAILRVKSVRRVRPDMESRDNASVYLSRPHVITFGTIFLAGLRSD
                     EGMISVLAHELMHIADGDNDSLRPLVSAVGKKASELTGLDVHGQRGEEITCDLIGAMA
                     VRAYIVDTPGYESIARRLARSIQHNCVEIDEGDEDHLSPRNTIRALLALNPALVRELI
                     NDRF"
     gene            complement(2209..>2996)
                     /locus_tag="VFO72_05755"
     CDS             complement(2209..>2996)
                     /locus_tag="VFO72_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9478826.1"
                     /translation="LFKIAMAYQNIHFRLEDRVARITFARPPLNVFNIEMMREISRAL
                     GECARRDLVAIVFDADKNCRAFSAGVAVEDHVQDTIFQMLDAFHSIFRVLEQLAKPAI
                     ALVDGAALGGGCELVAACDIVIASDRSRFGQPEIKLGVFPPVAAVLLPLVIGEKRARE
                     LILTGEIIDSVEAGRLGLCNHIVPGTQLEPKLLEVLAKLRELSGTSLAFARQSLDLGR
                     GRSIDAVLTEQENIYLHELMKTEDANEGVKAFLEKRKPVWRHR"
BASE COUNT          628 a          847 c          867 g          654 t
ORIGIN      
        1 gtcggcctga tcttatttct gatgatcttc agacgccgcc cacgctgacg atattttgtg
       61 ccttttgtgc ctttttgtgg ctaactgttc gttcgccgct ttgaggctca cttgagtatc
      121 gcggcttgtg cgctgatggt gagcgtgtgc catcgttgaa aggtattgat ggtctcgctt
      181 ggcatgacgc gaacgaacgc ggtctggaca tagagtgttt cgggcgcgcg ccagtcgagc
      241 ctcacgatcg gattgaacga cggcggcacg cctcctggta ccggtgtaga ttctggcgcc
      301 ggtgagtctg cattcgtcga agcttttttg gtggtggtcc tctgttcgta agcgagggag
      361 gctgcaacca gctgatcgcc gagtggcatt cgcgcctgcg tcggagtctt tcctacggcg
      421 tcgtaaatcg cgacgtcagt gtcgaacgca gtcgcgatct tcgaagcatc aggcgaccag
      481 acaggcagcg cgtcagtcaa tgcatcgtca agcaaacgtt cgctgccgct gggagcgatc
      541 aaacgcgggc gtccggccgg caacttaaac tgccgctcgc gcgcgttcca ctcgtcttcc
      601 ttgcacgcca gcgccgccat tgcgtgccca tcaggcgccc aaacaaagta gaagtagatc
      661 aacccttcgc gtgaagtcag cggcttcaga tcgtatccgt cgcgattgca gatatagatc
      721 tgttcggtgt taaagcttgc aactggcgca aacaatggcg caaccgaagg cgatggaaca
      781 ggcggggcta atggatccgg ctcgggttcg ttcggaggcg tcggagttgg gctcggcatg
      841 acgcgcttgc gcgcgatgaa gttgatgaag tttccgtcgg gcgaccagct aacagtttcc
      901 ggaaacacgc aggcaatatc cggcgggatc agattgcgca gaaactgtcc ttccgagttg
      961 tacagatcga tgcgaaatgt cgagcctggc tcgtcggggg tgccgtaaag agcgagcact
     1021 ctgcgtccat caggagaggc gacggtgcgt acgagtgcgt cgtctttgcg ccccgtcgag
     1081 aagtggttcg cgactgcgcc attcttgtcc gccggatctt caacagttga aggtgaatca
     1141 acatccggag acaagcggta agcaagattg cgcgcgggca cgtcgcgcag gaccttcgga
     1201 cgtacatcct gccgctcaat gacggattta catcctgtcg caaggaataa agtaaggaaa
     1261 attgtggttg cggcggaaat cgtagcagtt atccgtctca tcttgtggct agaaacgatc
     1321 gttgatcaat tctcgaacca acgccgggtt cagcgcgagc aacgcgcgaa tcgtgttgcg
     1381 cggcgaaagg tgatcttcgt cgccttcatc aatctcgacg cagttgtgtt gaatcgagcg
     1441 cgctaaccgc ctggcgatcg attcgtaccc gggtgtgtcc acgatgtacg cgcgaacggc
     1501 cattgccccg atcagatcgc acgtgatctc ctcgccgcgt tgaccgtgaa cgtcgagacc
     1561 ggtgagttcc gaagctttct ttcccaccgc ggaaacaagc gggcgcaaac tgtcgttatc
     1621 gccgtccgcg atatgcatga gctcgtgggc gagcacgctg atcattccct cgtccgaacg
     1681 caagccggcg agaaagatcg tgccgaaagt aatcacatgc ggccggctca gatacacgga
     1741 agcgttgtcg cgcgattcca tgtcggggcg gacgcgtctg actgatttga ctcgcaggat
     1801 cgcctggttg atgcggacct tgaagcgagg agatctttta aaaccatact ctgttgagag
     1861 ctggcgcaga gcggtgatga ccagcgtctt ggcaagcggc agcagacgct gcgcgcgctc
     1921 ggcgccggac cgcgcctccg ggctatgcac cggcaacgat ggacgcgcct gcatctcgtc
     1981 aatggcgaca ctgctcttcg ggtcgagctt ggcttgagta caaaccaccc caatcgcatc
     2041 ttctaacaac tccttcgcct cggccgacat cgtggggcgc gaacgcgttg aatgcgattc
     2101 gggtcgatcc tgcgaaaacg cgttgggtaa agacgccagg atcagcaaca caacacaaaa
     2161 caatcttttg tgtcttatgt gcctgtttgt ggctttgatc ttcattcttt atctgtggcg
     2221 ccaaacaggt ttgcgctttt ccagaaacgc cttcacgcct tcattcgcgt cttcagtctt
     2281 catcagctcg tgcaggtaaa tgttctcctg ctcggttaac acggcatcga tcgatctgcc
     2341 gcgtccgagg tccagcgatt gtctcgcaaa tgccagcgag gtgccagaga gctctctcaa
     2401 tttcgccagc acctcgagca gttttggctc cagttgcgta ccggggacaa tgtggttgca
     2461 caagccgagt cgcccggcct cgacagaatc gatgatctcg cccgtaagta ttaattcgcg
     2521 cgcgcgtttt tcaccaatca cgagtggcaa aagcacagca gctaccggcg gaaacacgcc
     2581 caacttgatc tccggttgtc caaaacgcga acgatccgaa gcgataacga tgtcgcacgc
     2641 ggcaacgagc tcgcaaccgc cgccgagtgc cgcgccgtcg accaacgcta tcgcgggttt
     2701 tgctaactgt tcgaggacac ggaagatcga gtgaaacgca tcgagcatct ggaagatcgt
     2761 gtcctgcacg tgatcttcaa ccgcgacacc ggcgctgaag gcgcgacaat tcttgtccgc
     2821 atcaaacacg atcgcgacca ggtcgcgccg cgcgcactcg cccaacgccc gtgaaatctc
     2881 gcgcatcatc tcgatgttga aaacattcaa cggcggacgc gcaaaggtga tcctcgccac
     2941 gcgatcttcg agacgaaagt ggatgttttg ataagccatc gctatcttaa atagga
//
LOCUS       DASTEY010000326         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_616868, whole genome shotgun sequence.
ACCESSION   DASTEY010000326 DASTEY010000000
VERSION     DASTEY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616868"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..686)
                     /locus_tag="VFO72_05760"
     CDS             complement(33..686)
                     /locus_tag="VFO72_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478827.1"
                     /translation="MNYKLLALLIACMFGPAAVRSQEPTAFELVKLSVEAMGVQQCIQ
                     IIDENREQVWSTVQVRFRITNVSKHPLIIYRYSPAMYDARLSRTLADMPARKFQFAER
                     PSLSHVPPRNFDEAQSTNEFRILQSNESFAYNPPDVLKFGSNELEDPDRRLFEGDYFL
                     QVKVATWVWEAEKAEKLQLRWAGFGKFFYDDVTSEPFPITIPKPGATTPRCDTLSVK"
     gene            complement(718..2187)
                     /locus_tag="VFO72_05765"
     CDS             complement(718..2187)
                     /locus_tag="VFO72_05765"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET9478828.1"
                     /translation="MTQAPEARSGLDAWRIAELPSAPRPHGLGWFSAVGPGVIALGVS
                     IGSGEFLLGPAAFVKYGLSLLWVVGVAALLQTLLNVELMRYTMATGEPVFTGFMRTRP
                     HSTFWAWFYSILYFLQMGWPGWAGAAAGAVFFLFAKRLPVTAEAGTVYAIGVGLFVLC
                     ALILTFGRRIERTLEYLNWIMVALIIGGLGTLAVLFVPGSTWVAAVAGFAGFDVQRGG
                     FQFLPEGADFFLLGAFAAYAGGGGVANLTLSNWARDKGYGMSSMVGFIPAAVGGKKVA
                     LAHSGCTFKPTASALERWRGWWRIIRVDQWCVYFAGAILGMAFPALLYVTFLPAGSDI
                     RGLGIAAALAQAVATQKGVLFGGAIAVLGVWILFKTQLDILEGMTRAITDILWTGSSR
                     IRAWRGGDVRIVYYAVLAMITAWGIIALRLTQPIILLSLSANMAGIVFIISSLHLLYV
                     NTRLLPLELRPPMWRRVALVALALFYGLFLTLWLWSVFD"
     gene            2478..2945
                     /locus_tag="VFO72_05770"
     CDS             2478..2945
                     /locus_tag="VFO72_05770"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9478829.1"
                     /translation="MSEIRIANPSDAEKITAVINAAFRIVEGFFIDGDRITLAEVQQS
                     LTTGAFLVAEDEDGIAGCVYVEPRGERAYLGLLSVDPARQQSGLGSRLMAAAEDFCRA
                     RGASFMDIYIVNLRTELPSFYERRGYTENGTTPFPADVPTKRPCHFINMSKPL"
     gene            2958..>3808
                     /gene="ligD"
                     /locus_tag="VFO72_05775"
     CDS             2958..>3808
                     /gene="ligD"
                     /locus_tag="VFO72_05775"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9478830.1"
                     /translation="MAKTDDKAEILSINGRDVRVSNPDKLYFSKQIKLTKLDLVRYYL
                     SIAPGALAGIRDRPIVLKRYVDGAEGEAFYQKRAPANRPEWLRTVTLSFPSGRYAEEI
                     VVDDAAGLAWIVNLGCIELHPHAVRSNDLDHPDELRVDLDPNPGVGWDDVRKVALEVN
                     SLLGEMGLRGWPKTSGSRGMHINVRIQQRWTFSEVRRAAVALSREIERRLPKLATSKW
                     WKEERHGVFLDYNQNAKDRTTCSAYSVRPLPDARVSMPLRWEEVEDCDPGDFNVLTVP
                     PRFAELG"
BASE COUNT          897 a         1083 c         1078 g          750 t
ORIGIN      
        1 tagcagcagg agaatggtga agactttgtg gatcacttga ctgatagagt atcgcagcgt
       61 ggtgttgtgg ctcctggttt aggaatagta atcggaaacg gctcagaggt cacatcgtca
      121 taaaagaatt tgccgaaccc ggcccagcgt agttgcagtt tctctgcttt ctccgcttcc
      181 caaacccagg ttgcgacctt gacttgcagg aagtagtcgc cttcgaacaa ccgcctgtct
      241 ggatcttcga gttcgttaga cccaaactta aggacgtccg gagggttata agcaaaagac
      301 tcgttcgact gcaaaatgcg aaactcgttt gttgattgag cctcgtcgaa gttgcgaggt
      361 ggaacatgag aaaggcttgg gcgttctgcg aattggaact tcctcgcagg catgtcagcg
      421 agagtcctgc tcagccgggc gtcgtacatc gccggggagt aacggtaaat gatcaaaggg
      481 tgtttactta cattcgtaat gcgaaagcgg acttgtaccg tcgaccacac ctgctcccgg
      541 ttctcgtcta tgatctgaat gcactgctgg acccccatgg cttcgacaga caactttacg
      601 agctcaaaag ccgtcggttc ctgggagcgc accgcggccg gaccaaacat gcacgcaatc
      661 agtaaagcga ggagcttgta gttcacagcg agatcatcat tcccatcgtg gacagtgtca
      721 atcgaaaact gaccacagcc acagcgtcaa aaacaaccca taaaaaagcg ccaacgcaac
      781 gagcgcaaca cggcgccaca ttggtggccg caactccagc ggaagcagac gcgtgttgac
      841 atatagtaaa tgcagcgagc tgataatgaa aacgattccc gccatgtttg cgctcagcga
      901 aagcaggatg atcggttgtg ttaaccgcag cgcaatgatg ccccacgccg tgatcatcgc
      961 cagcactgcg taataaacaa tgcgtacgtc gccgccgcgc cacgcgcgaa tgcgactact
     1021 gccggtccag aggatgtcgg tgatggcgcg cgtcatgcct tcgagaatgt ccaactgcgt
     1081 cttgaataag atccacacgc cgagaactgc gatcgcaccg ccgaacagca cgcccttctg
     1141 tgtcgccact gcctgggcca acgcggcggc gattccgagg ccgcgaatgt cgctacccgc
     1201 cggaaggaac gtgacgtaaa gcagtgcggg aaacgccata ccgagaatcg cgccggcaaa
     1261 gtacacgcac cattgatcga cacgtatgat tcgccaccag ccacgccagc gttccagcgc
     1321 cgaggccgtc ggtttgaacg tgcaaccgga atgcgcgagc gcgactttct taccgccgac
     1381 cgccgccgga atgaagccga ccatgctgct catgccgtaa cctttatcgc gcgcccagtt
     1441 gctcaaggtg agattcgcca cgccgccgcc acccgcataa gccgcaaacg caccgagcag
     1501 aaagaagtca gcaccctcag gcaggaattg aaacccacct ctctgcacgt caaagcccgc
     1561 gaagcctgcg acagccgcaa cccacgtcga tccgggaaca aaaagcacag ccaacgtacc
     1621 cagcccaccg atgatcaatg cgaccatgat ccagttgaga tactcgagtg ttcgttcgat
     1681 gcgccgtccg aacgtcagga tcagtgcaca caacacgaac agaccgacgc caattgcgta
     1741 gaccgttccc gcttcagccg taaccggcaa tcgttttgca aacagaaaga agacagcacc
     1801 tgcggcggcg ccggcccatc cgggccaacc catttgcaga aagtagagaa tcgagtagaa
     1861 ccacgcccag aaagttgagt gtgggcgagt gcgcatgaaa ccagtgaaca cgggttctcc
     1921 cgtggccatc gtgtagcgca tcaactcgac gttgagcaac gtctgcaaca aggcggcgac
     1981 accaaccacc cacaacaacg acaggccgta cttcacaaac gccgccggac cgagcagaaa
     2041 ttcgccactg ccgatcgaga cgccgagtgc aatcacgcct ggaccaaccg cgctaaacca
     2101 gcccagtccg tgcggtcgtg gtgctgacgg aagttcggca atgcgccagg catctagtcc
     2161 ggaacgcgct tcgggtgctt gcgtcatgag tccttcactt tggcttgcct caacagtccg
     2221 ctctcattct caccggggct tttagcccgg tgcaggagca gtgaagacgg agaaaaccgt
     2281 ttcaacggtt tttcgtggat tcgccagcga aaccgttaaa acggtttctc aaaaccgcgt
     2341 ggcggtaaca ccgggctaaa gcccggtgag aatgaaagaa gactttcaag gcagagcccc
     2401 ttcacttgat ctgagtttga caactactcc agaattgcgg cgcattctac catttgactg
     2461 gtccataaca actggagatg agtgaaatcc ggattgccaa tccgtcagac gcggagaaga
     2521 ttaccgcggt catcaatgcc gcgtttcgca ttgtcgaggg gttctttatc gacggcgacc
     2581 gcatcactct cgcagaggtg caacaatcgc tcacgaccgg cgcctttctg gtagccgaag
     2641 acgaggacgg aattgctggt tgtgtttacg tcgagccgcg tggtgaacgt gcttaccttg
     2701 gactgctatc ggtcgaccct gcgcgccagc aaagcggact cggctcgcgt ctgatggcgg
     2761 cggccgagga cttttgccgc gcgcgcggcg ccagtttcat ggatatctac atagtcaatc
     2821 tgcgcacgga actgccctcg ttttatgagc gccgcgggta caccgagaac ggcacgacac
     2881 cgtttccggc agacgtgccg accaaacggc catgccattt catcaacatg tccaagccac
     2941 tataattcgt tcattctgtg gcaaagactg acgacaaagc agagatcctc tcgattaacg
     3001 gacgcgacgt ccgcgtcagc aatcccgaca aactttattt ctcgaaacag ataaaactca
     3061 ccaaactcga tctggtgcgc tactacctgt cgattgcgcc gggcgcactc gccggaattc
     3121 gcgatcgtcc gatcgtgctg aagcggtacg tcgatggcgc tgaaggtgaa gcgttctatc
     3181 aaaagcgtgc gcccgcgaac cggccggagt ggttgcgaac cgttacgttg tcgttcccgt
     3241 caggtcgata cgcggaagag atcgtcgtgg atgatgcggc tgggctggct tggattgtga
     3301 atcttggatg catcgagttg catcctcatg cggtgcgctc gaacgatctg gaccatccgg
     3361 atgagttgcg cgtcgatctc gatccgaatc cgggtgtggg atgggacgac gttcgcaaag
     3421 tggcattgga agtcaactcg ctgcttgggg agatgggtct gcgtggttgg cccaagacga
     3481 gtggctcgcg cgggatgcat attaacgttc gcatccaaca gcgctggacg tttagtgaag
     3541 tgcgacgggc ggcggtggcg ttgtcgcgtg agattgagcg gcgactgccg aaactggcga
     3601 cgtcgaagtg gtggaaagag gagcgacacg gcgtgtttct ggattacaac cagaacgcca
     3661 aagaccgcac aacgtgttcg gcgtattccg tgcgcccgct gcctgatgcg cgcgtgtcga
     3721 tgccgctgcg ttgggaggaa gtcgaggatt gcgatcctgg ggatttcaac gtgttgaccg
     3781 tgccgccgcg ttttgctgaa ttgggtga
//
LOCUS       DASTEY010000327         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_501511, whole genome shotgun sequence.
ACCESSION   DASTEY010000327 DASTEY010000000
VERSION     DASTEY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501511"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFO72_05780"
     CDS             complement(<1..1136)
                     /locus_tag="VFO72_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET9478831.1"
                     /translation="MIKKLFAVAAATGLLISLAGSTPAFNVEKDSNRDHPGQQVERSM
                     AVDSQATITLCVAYGTLTVRGWDRPEVRVRSRDAEQIEFRRIDKPKEPNVPAHRVDVM
                     LLDKASRANPRRDCQAVASVEMEVPSGATVQVQTRDGNIRISGVAAAYAGSQNGDITI
                     ERATKLVEAGSVGGSIFLRDSSGRVSLNSAGGGVEASNVRAADADDTFEVGTVSGDIQ
                     LDRIKSPKVMAKTVNGSLTMSGALVKSGYYAFTNMTGDVLLALPHDASFRLNAKLSEK
                     RDIVSDFVLKYVGETPPPTPPAPRPAVVPAPSAEAAITPVPKKDAKTPAAKAPKTPAA
                     KEKEKPPLKAGTAVTPPLVVIERPTVVVAPYVRRVEAICGSGDAT"
     gene            complement(1153..2013)
                     /locus_tag="VFO72_05785"
     CDS             complement(1153..2013)
                     /locus_tag="VFO72_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9478832.1"
                     /translation="MTRCLDQATLQSYFDGELSGEMMERASLHLASCSTCAAAAREME
                     EEIGLLMTALAPDFEASVPTERLRQQIDAAVLDLRIGAASSRERTGFGAFISSLLSFG
                     TQRTLGYASLVMVLAFAAIFGLVKFKTTPQEPIAETQPVMNADKQVAQTPPEAPAAFE
                     PVQVNTPVARNNTDKRAGIKRNRTPKSELIKSEPVKLLPGERSYLQTIARLDSTIKAN
                     QKSMRPSLQVEYERNLAVVDRAIAATRSAAKSNPNDPDAADFMFAAYQSKVDLLNTIA
                     DARLGGGRSQ"
     gene            complement(2010..2552)
                     /locus_tag="VFO72_05790"
     CDS             complement(2010..2552)
                     /locus_tag="VFO72_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9478833.1"
                     /translation="MPNDIGTEAISPLSFDEAFTLHHRAVFRTARAVVRDSALAEDVT
                     QEVFLRLYRNLHATPGEELLRAWLLRVTLNVARNTVRGQTRSMTRDNEYHKSTVENGI
                     SVDAPEEDYERRLAIEEARRALDKVKEPMRSCLLLKQQGLSYREIANSLSLKETNIGS
                     LVARGRKEFARVYGKIGGRR"
BASE COUNT          586 a          861 c          779 g          680 t
ORIGIN      
        1 gtcgcgtcac ccgaaccgca gatcgcttct acgcgccgca cgtaaggagc gactaccact
       61 gtcggcctct cgatcaccac gagtggcggc gtgactgctg ttccggcttt caacggcggt
      121 ttctctttct cttttgccgc aggtgtcttg ggtgctttgg ccgccggagt ctttgcgtct
      181 ttctttggga ccggcgttat cgccgcttcc gcggacggcg ctggaactac ggccggtctc
      241 ggtgcgggag gagtcggtgg gggtgtttct ccgacatact tcaaaacaaa gtcagaaacg
      301 atgtcgcgct tctcggagag ttttgcgttc aggcgaaagg aggcgtcgtg cgggagcgcc
      361 agcaagacgt cgccggtcat gttggtaaat gcgtagtaac cggacttaac caaggcgccg
      421 ctcattgtca gactgccgtt caccgtcttg gccatgactt tcggactctt gatccggtcc
      481 aactgaatgt cgccgctgac cgtaccaact tcaaaagtat cgtcagcatc ggccgcgcga
      541 acgttagaag cctcgacacc gccgcccgcg gaattcaaac ttacgcggcc gcttgagtcg
      601 cggaggaaaa tactgccccc gacgcttccc gcttcaacca atttcgtagc gcgctcgatc
      661 gtgatgtcgc cgttttgcga accggcatac gcggcggcca ctccagaaat cctgatgttg
      721 ccgtcgcgcg tctgaacctg aacagttgcg cctgaaggca cctccatttc gaccgaggca
      781 accgcctggc aatcgcgtct gggattagcg cgtgacgcct tatcgagcag catcacgtcg
      841 acgcggtgcg ccggtacgtt tggctctttc ggtttgtcga tacggcgaaa ctcgatctgt
      901 tccgcgtcac gtgaacgaac gcgaacttcc ggcctgtccc aaccgcgcac cgtcagcgtg
      961 ccgtaggcaa cacacagcgt aatcgtggct tgtgaatcca cggccataga ccgttcgacc
     1021 tgttggccgg gatggtcgcg gttggaatcc ttctctacgt taaaagcggg ggttgaacct
     1081 gccagggaga ttaagaggcc ggtagccgcg gcgactgcaa acaacttctt gatcaactta
     1141 gaacccggcc tttcactgtg agcgacctcc gccgagacgc gcatcggcga tggtgttgag
     1201 caaatctacc ttgctctggt aagcagcaaa catgaaatcg gccgcgtccg gatcgttcgg
     1261 attgctcttg gctgcactgc gcgtcgcagc gatggcgcga tcgaccaccg ccagattgcg
     1321 ctcatactca acctgtaagg acggtctcat tgatttctga tttgctttga tcgtggaatc
     1381 gagacgagcg atcgtttgca gataagaacg ttcgcccgga agaagtttga ctggttcaga
     1441 cttgattagt tcagacttag gagttctgtt tcttttaatt ccggctctct tatccgtgtt
     1501 attccgtgct accggggtgt taacttgaac cggctcaaac gcagcaggcg cttcaggagg
     1561 ggtttgcgcc acttgcttgt ccgcgttcat gactggttgt gtttcagcaa taggttcctg
     1621 tggcgtagtc ttaaacttca ccaatccaaa gatcgcggca aacgccagca ccatcacgag
     1681 actcgcataa cccaacgttc gctgtgttcc aaaactgagc agactcgaaa tgaaggcgcc
     1741 gaatccggtt cgctctctcg acgacgctgc tccgattcgc agatccaaaa cagccgcgtc
     1801 gatttgctgt cgcagccgtt ccgtcggcac actggcttca aagtccggcg ccagagccgt
     1861 catcaacaaa ccgatctcct cctccatttc gcgcgctgcc gcagcgcacg tggagcatga
     1921 agcgagatgc aggcttgcgc gctccatcat ctcgccggaa agctcaccgt cgaaataact
     1981 ttgcagcgtt gcctggtcca aacaccgtgt catcgtctgc ctccgatctt cccgtagacc
     2041 ctcgcaaact cctttcgacc gcgagccacc agactgccga tatttgtttc tttcagcgac
     2101 aggctgttgg cgatctcgcg atacgaaagc ccctgttgtt tgagcaacag gcagcttctc
     2161 atcggctctt ttactttgtc caaagcccgg cgagcttctt cgatcgccag acggcgctcg
     2221 tagtcctctt ccggtgcgtc gacagagatg ccattttcta cggtgctctt gtgatactcg
     2281 ttgtcgcgag tcatcgagcg ggtttgaccc cgcacggtgt ttcgcgccac gttaagagtc
     2341 acgcgcagaa gccacgcgcg aagcagttct tcgcccggcg tagcgtggag attgcgatag
     2401 agtcttaaaa atacttcctg cgtcacgtct tccgccaggg cggagtcgcg gacgacagcc
     2461 ctggccgttc taaaaacggc gcggtgatgg agggtaaacg cttcgtcgaa actgagcgga
     2521 ctaatcgctt cagtcccgat gtcgttaggc atggactcga tcaaagctct agcttgtccg
     2581 ttcatcaatc accgctctct caacagtgca aacaccacaa accactgact tgtggcaagt
     2641 tttctaacaa atcgccaaag tcagccagcc agagtggttt gagcgtaaat attgtagtcc
     2701 cgaattccgc tccgcaccaa tccatgaaaa cagtcagtag tgtcctaatc tgaacgccag
     2761 agggttcgct aattccagct gaacgccaga ggcgttcgct aattccagcc cagggttgct
     2821 tcaaccctgg gtcgaacgtt agcgtcccgt ggctttgcct caaaagtcgc tctctcattc
     2881 tcaccgggct tcagcccggt gttact
//
LOCUS       DASTEY010000328         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_270801, whole genome shotgun sequence.
ACCESSION   DASTEY010000328 DASTEY010000000
VERSION     DASTEY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270801"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3270)
                     /gene="mfd"
                     /locus_tag="VFO72_05795"
     CDS             complement(<1..>3270)
                     /gene="mfd"
                     /locus_tag="VFO72_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9478834.1"
                     /translation="LRFWYAALAGKQNAENEVLVLPASEIDPYAGISPHAQTLERRAL
                     ALWRLQRQAPSFVLLTARALARKTVGPAAVARAGTVLRRDEDHSPEDLVEKLMATGYV
                     REDPVAAVGEFSLRGGIVDIWPPGRETPVRLEFFGDTIDSMREFDPENQLSTGQLRVV
                     EVPPMREFWATALDFQLWSEAARERWNDERFDRSLRDRTAFADEGEPFAGWEWLISIS
                     TDCTSTVFDHLGDAVLVVDEPAGIESYLSEFYERLEDRYREIDQADDLALRPEELFLS
                     DEDLRRLIETRQRIELRTLGRAAAETDLNLALDAEAPKVQLGRDRSARRPAFVFPVVE
                     TAFEVEWQAQSAMRYHGRIADLAADVKRAAEENRTMLFVMPSLGVAERISEILSEYQI
                     DARLALTGETSDAAGSRPVVITVGRLSSGFELPKSWLIVHVESDVFDETVETVERRGP
                     TEARFKPEGKKRRSKAAAFLSDFRDLKPNDYVVHIDHGVARFGGLQTLDLGQRTGEFM
                     LLFYADDAKLYVPVERLDLVQRYSSAEGHQPQLDRLGGLGWQKAKAKAKRAIRDMADE
                     LLRLYAERKLVGGFAFSSDTPWQHEFEDGFPFVLTPDQETAIEDVKKDMEEPVPMDRL
                     LCGDVGYGKTEVAMRAAFKAVMEGKQAAVLTPTTVLAYQHYDTFRTRFAPFPVKVELL
                     SRFRSTKEQKEVVKRVETGEIDVLIGTHRMLSKDVAFKDLGLVVVDEEQRFGVAHKER
                     LKHLKKKVDVLTLSATPIPRTLNMSLTGMRDMSLIETAPRDRLAIQTQVVQFSDSVIK
                     SAIDLELSRGGQVFFIHNRVETIETIAALVKRLVPQARLAVGHGQMNEKEMERVMLDF
                     IDYKYDVLVATTIIENGIDIPRANTIIINRADQYGLSQLYQLRGRVGRSNRRAYAYLL
                     IPGEQELSPIARRRLAAIREFSDLGAGFRIAALDLELRGAGSLLGGEQSGHMETLGFD
                     LYTQMLERTVAELRGEEVEDETSVALNLGVDVAIPDDYISDMGQRLRTYKRVSSARDE
                     ATLTAIRTETEDRYGRVPLSLDRLFAYARLRRLAEELGLLSIDRTPDGIAIKFSEKAR
                     I"
BASE COUNT          681 a          981 c          916 g          692 t
ORIGIN      
        1 aaatacgcgc cttttcacta aactttatcg ctattccgtc cggcgtccga tcgatcgaca
       61 gcaggccaag ctcctcggcc aaacggcgta aacgggcata ggcaaatagt cgatccaacg
      121 acaacggcac acgcccgtaa cgatcctcag tctccgtccg gatggcggtc aatgtggcct
      181 cgtcccgagc ggaagagacc cgcttgtagg tccgcagccg ctgccccatg tcggagatgt
      241 aatcgtctgg gatggccaca tccacgccaa gattcaacgc gacactggtt tcgtcttcca
      301 cttcctcgcc gcgcaattcc gcgaccgttc gttccagcat ttgcgtgtaa agatcgaacc
      361 cgagcgtctc catatggccc gattgctcgc ctcccaaaag gctccctgcc ccccgcagtt
      421 ccaaatcgag cgccgcgata cgaaaacccg ccccgagatc cgaaaactca cgaattgctg
      481 ctaaacgacg gcgggcaatg ggtgaaagtt cctgctcgcc cggtatcaga agataggcgt
      541 aagcgcgccg gttagaacgg ccgacccgcc cgcgcagttg ataaagctga gaaagaccgt
      601 attgatcggc gcggtttatg atgatggtgt ttgcgcgcgg gatgtctatt ccgttctcta
      661 tgatggtggt tgcaacgaga acgtcatact tgtaatcgat gaagtcgagc attacccgct
      721 ccatctcctt ctcattcatc tggccatggc caacggccag acgcgcctgc gggaccaggc
      781 gtttgaccag tgcggcgatg gtctcgatcg tctcgactcg attgtgaatg aagaacacct
      841 ggccgccgcg agacagctca aggtcgatag ccgacttgat tacgctgtcg gaaaactgca
      901 cgacttgagt ttgaatcgcg agtcgatcgc ggggcgcagt ttcaatcaac gacatgtcgc
      961 gcatgccggt gagtgacatg ttcagcgtgc ggggaatcgg cgtcgccgac agtgtcaaca
     1021 cgtcgacttt cttcttcaaa tgtttcagcc gttctttatg ggccacgcca aaacgctgtt
     1081 cttcatcgac caccaccaat ccgagatctt tgaacgccac gtccttcgac aacatgcggt
     1141 gcgtgccgat gagcacgtcg atttcgccgg tctcgacgcg cttcacgact tccttctgtt
     1201 ccttcgttga gcgaaagcgc gacaagagtt ccaccttcac cggaaacggc gcgaagcgcg
     1261 tgcgaaacgt gtcgtagtgt tgataggcca ggacggtggt cggcgtaagc accgccgcct
     1321 gttttccctc catcacggct ttgaacgccg cgcgcatcgc aacttccgtc ttgccgtaac
     1381 cgacgtcgcc acacagcaga cggtccatcg gtaccggttc ttccatgtct ttctttacgt
     1441 cttcaatcgc cgtttcctga tcgggcgtga gcacaaacgg aaagccgtct tcgaactcgt
     1501 gttgccatgg agtgtcggaa gagaaagcga agccaccgac gagtttgcgc tcggcgtaaa
     1561 gacgcaacag ttcgtcggcc atgtcgcgaa tggcccgctt cgctttggct ttggctttct
     1621 gccagccgag cccgccgaga cgatcgagtt gcggttgatg accttcagcg ctcgaataac
     1681 gttgcaccag atcgagccgc tcaaccggga cgtagagctt tgcatcgtca gcgtagaaca
     1741 gcagcataaa ctcgccggtg cgctggccca ggtcgagagt ttgcaaaccg ccaaaacgcg
     1801 cgacgccgtg gtcgatatgg accacgtagt cgttgggctt cagatcccta aaatccgaca
     1861 gaaacgccgc cgcttttgac cgacgtttct ttccctcggg tttaaatctg gcttcggtgg
     1921 ggccgcggcg ttcgactgtc tctaccgttt cgtcaaaaac atccgattca acatgaacaa
     1981 tcaaccacga tttcggtaac tcgaatccgc tgcttagccg gccgactgtt atcacgaccg
     2041 ggcgtgagcc ggccgcgtca gaagtttcac ctgttaacgc gaggcgcgcg tcgatctgat
     2101 actcactcag gatctcggaa atgcgttcgg ccacgccgag actcggcatg acaaacaaca
     2161 tggtcctgtt ctcttcagcc gcgcgcttca cgtcggccgc gagatccgca atgcggccgt
     2221 gataacgcat tgcggattga gcctgccact cgacttcaaa agccgtttcg acgaccggaa
     2281 aaacaaacgc cggacggcgc gcagatcgat cgcggccaag ttgaactttt ggcgcttcgg
     2341 cgtcgagtgc gagattcagg tcagtttcgg cggcagcccg cccgagtgtg cggagttcga
     2401 ttcgctggcg ggtctcaatg agccgccgca gatcctcgtc acttaaaaac agttcttccg
     2461 gtcgtagcgc gaggtcgtcc gcctggtcga tttcgcgata acgatcttcc aggcgttcgt
     2521 aaaactcgct gagatacgac tcgatacctg cgggttcatc gacaacgagg accgcatcgc
     2581 cgagatgatc gaaaacggtc gaggtgcagt cggtggagat tgatatcagc cactcccaac
     2641 ccgcgaacgg ttcgccttcg tccgcaaagg cagtgcggtc gcgtaacgag cggtcaaaac
     2701 gttcatcgtt ccaacgttcg cgcgcggctt cactccaaag ttggaaatcg agtgcggtgg
     2761 cccagaactc gcgcatgggc ggaacttcca ccacgcgaag ttgaccggtt gagagttgat
     2821 tctccggatc aaactcgcgc atggagtcga tcgtgtcacc gaagaattcc agtcgcaccg
     2881 gcgtttcgcg tccgggcggc cagatatcga cgatgccgcc gcgcaatgaa aactcgccga
     2941 cggcggccac gggatcctca cgcacgtatc cggttgccat cagcttctcg accaagtctt
     3001 cgggtgagtg atcctcatcg cgtcttagca ctgttccggc gcgcgcgact gcggctgggc
     3061 caactgtctt gcgcgcgagt gcacgagcag taagcagcac gaaactcggc gcctggcgct
     3121 gcaagcgcca aagagcgagt gcgcgccgct cgagtgtttg tgcgtgcggt gaaatgccgg
     3181 catagggatc tatttctgac gccggcagga cgagcacttc gttttcagcg ttttgttttc
     3241 cggcgagggc tgcataccag aagcgcaggt
//
LOCUS       DASTEY010000329         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1375113, whole genome shotgun sequence.
ACCESSION   DASTEY010000329 DASTEY010000000
VERSION     DASTEY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375113"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFO72_05800"
     CDS             complement(<1..489)
                     /locus_tag="VFO72_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9478835.1"
                     /translation="MQKLAEICIRRPVFATMIVLSLVVVGAAGFFRLGVDRFPAVDLP
                     TVSVRVGLPGAAPEEVESLVTQPVEEVVNTVDGISELRSISGQGSSIVIATFNLDRDL
                     ESAAQDVRDRVSTLGRRLPEDATPPVVQKFDNDSTPVLTIALTADRSIRELTELGDKI
                     ARP"
     gene            complement(532..1743)
                     /locus_tag="VFO72_05805"
     CDS             complement(532..1743)
                     /locus_tag="VFO72_05805"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9478836.1"
                     /translation="MFQTRIPLLGVSLLLAALFLTSCKSDYQASTRQERGGDNRPARQ
                     VKTAVVSETPFGETVTANGTLAAYDQTTVSVKVPGRVRMINVDLGSVVSRGQVIALVD
                     SEDYRLRVQQAEASLAQARARLGLAPDGTDDRVDPEQTATVRQARAVLDEARHNRDRA
                     ARLVEQGVIAKAEFETATATFKVAEGRYQDAYEEIRNRQGILAQRRSELALARQQLKD
                     TAVVAPLDGIVQEKLTSIGEFLAAGAPVVNIVRMDPLRLRAEIPERESQTVRTGQDVR
                     VNVEGDPNVYVGKIMRLSPVIAEQNRMLVVEADVRNNGKLRPGSFAHAEIVTNDAKMA
                     VTVPNNAIVTFAGIEKVIVVQNGKAFEKPITTGRRSTDFTEIVAGISVGEKVIVDPGN
                     LQSGQAVEVVD"
     gene            complement(1845..>2112)
                     /locus_tag="VFO72_05810"
     CDS             complement(1845..>2112)
                     /locus_tag="VFO72_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478837.1"
                     /translation="IMTLRGTYQFTKATFARLILDYNTLNSRLRSQALFGWTPSPGTA
                     FYAGYNDDLNYDTLHPFTRNVVPGFRRNARTFFIKMSYLIRKGF"
BASE COUNT          460 a          619 c          563 g          470 t
ORIGIN      
        1 tggacgtgcg atcttgtcgc caagttcggt gagctctcga atggaacgat ccgcagtgag
       61 cgcaatcgtg agaacgggcg tgctgtcgtt atcaaatttc tggacgaccg gcggcgtggc
      121 gtcttcggga agtcttctgc ccagcgtgct gacgcgatcg cgaacgtcct gtgcggcaga
      181 ttccaggtcg cgatcgagat taaaggtggc gatcacaatc gatgagccct gacccgaaat
      241 agagcgcaac tcactgatgc catcgacggt gttgactact tcttcgaccg gctgtgtaac
      301 tagcgattca acttcctcag gtgcagcgcc cggcaaacca acacgaaccg agacggtagg
      361 aagatcgacg gcgggaaaac gatctacgcc gagcctaaag aaacccgccg cccccaccac
      421 aaccagcgac aacacgatca tcgtcgcgaa aacaggacgg cgaatacaaa tttcggcaag
      481 tttttgcatt aaaaatatag gtccctaccg gacctattcg acgtattcta gttaatcaac
      541 cacttcgaca gcctgccccg attgcaggtt gcccggatcg acgatcactt tttcaccgac
      601 gctgatgccc gccacgattt ccgtgaagtc ggtgctccgc cggccggtcg tgatcggttt
      661 ttcgaatgcc ttcccgttct ggaccacgat caccttctca atacctgcaa acgtaacgat
      721 cgcattgttc ggaaccgtta ccgccatctt cgcatcgttg gtcacgatct cggcgtgtgc
      781 aaatgaaccg ggccgcagct ttccattatt tcgtacgtca gcttcaacca ccagcatgcg
      841 gttctgctcg gctatcaccg gactgagccg catgatcttg ccgacataga cattcggatc
      901 accttcaacg tttacgcgaa catcctgacc cgtgcgcacg gtttgcgatt cgcgctccgg
      961 tatctcagca cgcaatctca acggatccat tcgcacaata ttgacgaccg gcgcgccggc
     1021 ggccagaaac tcacctatgc tggtgagctt ttcctgaaca ataccatcga gcggagcaac
     1081 cactgccgtg tctttcaatt gttgtcgggc cagcgctaac tccgatcggc gctgcgccag
     1141 aattccctga cgatttcgaa tctcttcgta cgcgtcctga tatcttccct ccgcgacttt
     1201 gaacgttgca gtcgccgttt cgaattcggc tttggctatg actccctgct ctaccaggcg
     1261 cgccgcgcga tcgcggttgt ggcgagcttc atcgagcacc gcgcgcgcct gccgcactgt
     1321 cgcagtctgc tccggatcga cgcggtcgtc cgttccatca ggcgccagcc caagtcgcgc
     1381 tcgagcctgt gccaacgaag cctcagcttg ttgcactcgc aaccggtaat cttccgagtc
     1441 aaccagcgcg atcacctgac cgcgcgacac tacgctgccg aggtcgacgt tgatcatccg
     1501 cacgcgcccg gggaccttca cactaacagt tgtttgatcg taagcggcga gggtgccgtt
     1561 ggcagtaacg gtctcgccaa acggggtttc tgaaacgacc gcggttttga cctggcgagc
     1621 gggccggtta tctccgccac gctcttgcct ggtcgaagcc tggtaatcac tcttgcacga
     1681 agtcaaaaaa agcgccgcca gcaagagcga cacccctagc aaaggtatcc gggtctgaaa
     1741 catcctttgc agtttatcat gcgggccttt gcgagtcccg aaaagccaca gtgatttagg
     1801 taaagttatg taaagttacg taaagcaatt gtaagttttt agggctagaa acctttgcgt
     1861 atcaggtacg acattttgat gaaaaatgtc cgtgcgttgc ggcggaaacc ggggacgaca
     1921 ttacgtgtga acggatgaag ggtgtcgtag ttcagatcgt cgttgtaacc ggcgtagaac
     1981 gctgtgccgg ggctcggcgt ccacccaaac aacgcctgcg atcgcaagcg tgagttgagg
     2041 gtgttgtaat cgagaatcag ccgcgcaaag gtggctttgg tgaactggta agtaccgcgc
     2101 agtgtcatga tc
//
LOCUS       DASTEY010000330         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1325438, whole genome shotgun sequence.
ACCESSION   DASTEY010000330 DASTEY010000000
VERSION     DASTEY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325438"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFO72_05815"
     CDS             <1..354
                     /locus_tag="VFO72_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HET9478838.1"
                     /translation="WLIDASGCDAAALADLDRMRAVFERVIRDLDLNVLGEIAWHKFD
                     HPGGVSGLALLSESHLTCHTYPEFQAATFNLYCCRNRASWTWETTLKEMLGATDVTVR
                     KFERMIAHTMAGAAK"
     gene            351..>2316
                     /locus_tag="VFO72_05820"
     CDS             351..>2316
                     /locus_tag="VFO72_05820"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HET9478839.1"
                     /translation="MSVLVANCPSCGAPIEFQSGQSIVKICEYCRSAVARTDRDLKDL
                     GKVAELVETGSPLDVGVRGKWKDVPFELTGRAQLGHEMGGQWDEWYATFSNGWLGWLA
                     EAQGRFYLTFQYPIPEGVEVPPFDQLQLGQSVPGLPWPTPLIVAETGRATALGAKGEI
                     PYLLTPGETYYYADLSGVNGAFGTLDYNQSPPLVFLGQQVTLADLGITTTRTPEREER
                     RVGAAHLSCPNCAGPLELRAPDKTERVTCPNCNSLLDVNQGQLQFLKALEKPWFQPLI
                     PIGTVGEVPEGKMTVIGAMARSVTIEGTQYFWSEYLLYHPQIGFRWLVQSDDHWNYVR
                     AVPPGEVSESPKSASYQGKSYKIFQDAQARVEVVLGEFYWKVEAGEQVRGVDYVKPPY
                     MLSKEVSTVYVTNPDNANRTQRVTGEINWSHGTYMDVPQIEKAFSVSGLPRPSGVAPN
                     QPYKHWWVYKYWLAFIVLTLLLGFVTLVFSGSTREVFSQTVTLPPLPNADGTQVFFSE
                     PFQLQGRRNIKIVGESSVQNAWVYLEGDLINDETGLVQSFPIDISYYQGVEDGESWSE
                     GGQTDSAYSSSMPSGRYVLRLEGQWERWQQPAVVTVKIEQNVTRGFNLLIALIILSIG
                     PIAMLIYHVSFEHKRWSESMFSSSGDDSDDD"
BASE COUNT          504 a          640 c          680 g          492 t
ORIGIN      
        1 tggctcatcg atgcaagcgg atgcgacgcg gcggcgcttg ccgatctcga tcgcatgcgt
       61 gcagttttcg agcgcgtcat acgcgatctc gatctcaacg tactcggtga gatcgcgtgg
      121 cataaattcg atcatcccgg cggcgtcagc ggtctcgcgc tgctctccga gtcgcatctc
      181 acctgccaca cgtatccgga attccaggcg gcaacgttca acctgtactg ctgtcgcaac
      241 cgcgcctcat ggacctggga aacaacgcta aaggaaatgc tcggcgcaac tgacgtgacc
      301 gttcgcaagt tcgagcgcat gattgcacac accatggcag gagccgctaa atgagtgtgc
      361 tcgtggcgaa ctgcccgtcg tgcggcgcgc ccatagagtt ccagagcggc cagtcgatcg
      421 taaagatctg cgaatactgc cgctccgcag tcgcacgcac agatcgcgac ttgaaagacc
      481 tcggcaaggt cgcggagctc gtagaaaccg gatcaccgtt ggacgtcgga gtgcgtggca
      541 agtggaagga cgtgccgttt gagttaaccg ggcgcgcgca actcggccac gagatgggcg
      601 ggcaatggga cgagtggtac gccacgtttt caaacggctg gctcggttgg ctggcggaag
      661 cacagggccg cttctacctc acgtttcaat atccaattcc cgaaggcgta gaggtcccgc
      721 ctttcgatca actccagctc ggacaaagcg tgcctggcct tccatggccc acgcctctga
      781 tcgtcgccga aaccggacgc gccacggctc tcggcgccaa aggcgagatt ccgtacctgt
      841 taacgcccgg tgagacttat tactacgccg atctctcagg cgtgaacggc gcgtttggca
      901 cactcgatta caaccaatca ccaccgctcg tcttcctggg ccaacaagtc acacttgccg
      961 acctcggcat cacaaccacg cgcacgcccg agcgcgaaga acgacgagtc ggcgcggctc
     1021 atttgagttg tccaaactgc gccggaccgc tcgaactccg cgcgccagat aaaaccgagc
     1081 gagtcacttg ccccaactgc aattcgctgc tcgatgtcaa ccaaggccag cttcagtttc
     1141 tcaaagcgct cgagaagccg tggtttcaac cgctgattcc tatcggtact gtaggcgagg
     1201 tgcccgaagg aaagatgacc gtcatcggtg cgatggcccg cagcgtcacg atcgaaggca
     1261 cgcagtactt ctggagcgag tatcttctct accatcccca gatcggcttt cgctggctgg
     1321 tccagagcga cgatcactgg aattacgtgc gcgccgtgcc acccggagaa gtttctgagt
     1381 cgcctaagtc tgctagttat caaggcaaga gttacaagat ctttcaggac gcgcaggcgc
     1441 gagttgaggt agtgctcggg gagttttact ggaaagtcga agcgggcgaa caggtgagag
     1501 gcgtcgatta cgtcaagccg ccttacatgc tttcaaaaga agtgtcgacg gtttacgtga
     1561 cgaatccgga taatgccaat cgcacgcagc gcgtcacggg cgagatcaac tggtcacatg
     1621 gaacttacat ggacgtgccg cagatcgaga aggcattctc ggttagcggg ctgccgcgtc
     1681 cttctggcgt cgcgccaaac cagccttaca agcattggtg ggtgtacaaa tactggctgg
     1741 cgttcatcgt gctcacgctg ttgctgggct tcgtgacgtt ggttttttcc ggttcgacca
     1801 gggaagtgtt ttcgcaaacg gtaacactgc cgccactgcc gaatgcagac ggtacgcagg
     1861 ttttcttcag cgagccgttt cagttgcaag gcaggcggaa catcaagatc gtcggcgagt
     1921 cgtctgtaca gaatgcgtgg gtttatctcg aaggagacct gatcaacgat gaaacaggct
     1981 tggtccaatc gtttccgatc gacatttctt actaccaggg cgttgaagat ggcgaatcgt
     2041 ggtcggaggg cgggcaaaca gattcggctt actcttcttc aatgccttcc ggtcgatacg
     2101 tactgcggtt ggagggacag tgggagagat ggcagcaacc ggcagtcgtg acagtgaaga
     2161 ttgagcagaa cgttacgcgc gggttcaacc tgctgattgc gttgataatt ttgtcgattg
     2221 gtccaatcgc gatgttgatc tatcacgtca gtttcgagca caagcgatgg tcagagagta
     2281 tgtttagtag tagtggcgat gatagtgatg atgatg
//
LOCUS       DASTEY010000331         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1095178, whole genome shotgun sequence.
ACCESSION   DASTEY010000331 DASTEY010000000
VERSION     DASTEY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095178"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFO72_05825"
     CDS             <1..744
                     /locus_tag="VFO72_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478840.1"
                     /translation="AREPDTYRGTLVFAAETEGGDKAIGIPTLSAEVAKSGNDRRLSF
                     KLPDGSDLVYLEKAGVQYGIAPARKQYAELTPEATGFQLQRLMTPGQLVQYLERLRGI
                     EFIGEEPLTGRTALKYRYARTAQTQTSAGEVKTESFVYVDKDTGLPLRAELFSEASGN
                     VQGVKGAKVVAEMRDIQTNVDPSLFEIPTGFNKVPEAQVRAQVNAVTNTVAAVLRTLL
                     TNMNTAPPPSPTPTGSPAASPSPSPTPTR"
     gene            755..1492
                     /locus_tag="VFO72_05830"
     CDS             755..1492
                     /locus_tag="VFO72_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478841.1"
                     /translation="MRYVVILLLGILLGGGAAIFFLGAPRAQLMQGQAVQPPAQGGNP
                     PGTVVIALEQSFIDAVLAATFSGLGTPTFQLGQNRNGSERLEEALFQGGCTNSITLLP
                     EGSGVKTGVQLRNGNVNAPMAFTGSYNLAGCMQFKGWAQSSIKLSFDQEKQTVFGYVT
                     VEGVNLEGVNPIANTLVTSFVQGAINQRVNPLVLVSEPQLSLMIPVKASNGTVKARAT
                     DVRAEVLDGSLKLYLTYEFMGVKGEGT"
     gene            complement(1511..>2025)
                     /locus_tag="VFO72_05835"
     CDS             complement(1511..>2025)
                     /locus_tag="VFO72_05835"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9478842.1"
                     /translation="RLLEEGKPLPDLIMIDGGKAQLNAAANATSELGLEAIPMVGIVK
                     PPQRHNDVAYLLMKDREDEPIYLDSHSLILRLMQMIRDETHRFAVTYHRKRRELRDFT
                     SELTAIPGVGDKRKGRLLRHFGSIQRISRAGVSELTPFVGRKTAEEIAGHFERQRLLA
                     ADYADKRESA"
BASE COUNT          460 a          536 c          586 g          443 t
ORIGIN      
        1 gctcgcgaac cggacaccta cagagggacg ctggtctttg ccgccgaaac tgaaggcggg
       61 gacaaagcaa tcggtatccc aactctgtct gccgaagttg cgaagagcgg aaacgatcgg
      121 cgactctcgt tcaagttgcc cgatggatcg gatctggttt atttggaaaa ggccggtgtg
      181 caatatggaa tcgctccggc gcggaagcag tatgcagagt tgacacctga ggccactgga
      241 tttcagcttc aaagactgat gacgccggga caactagtac aatacctcga acgacttcgc
      301 ggcatcgaat tcatcggcga agaaccactc acgggccgca cagcgctgaa gtatcgttac
      361 gctcgcactg ctcaaacaca gacgtcggcc ggcgaagtta agaccgaatc ttttgtttat
      421 gtcgacaaag acacgggact gccgttgcgt gcggaacttt tcagcgaagc cagtggtaat
      481 gtacagggtg taaaaggcgc caaggtggtt gcggagatgc gcgacattca gacgaatgtc
      541 gatccgagtc ttttcgaaat cccgactggt ttcaacaagg ttcctgaggc gcaggtgaga
      601 gcgcaagtga atgccgtgac gaacacggtt gcagcagtgc tgcggacgtt gttgacgaat
      661 atgaacactg caccaccgcc gtcgccaacg ccgactggat cgccggcggc cagtccaagt
      721 ccgagtccaa ctccaactcg ttgaaggtga acacatgaga tacgttgtca ttctcctgct
      781 tggaattctg ctcggcggcg gcgctgccat cttctttttg ggagcgccgc gcgcgcaatt
      841 aatgcagggc caggcagtgc agccgcctgc acagggagga aatcctccgg gcacggtcgt
      901 gattgcgctc gagcagagtt ttatcgatgc cgtattggcg gcgacgttct caggattggg
      961 aacgccgaca tttcaactgg gccagaatcg caacggttcg gagcgcctcg aggaggcgct
     1021 cttccagggt ggctgcacaa actcgatcac gctcttgcct gaaggcagcg gcgtgaagac
     1081 gggcgtgcag cttcgcaacg gcaacgttaa cgcgccgatg gcgttcaccg gcagctacaa
     1141 ccttgctggt tgtatgcagt tcaaagggtg ggcccagagc agtatcaagt tgtctttcga
     1201 tcaggagaag cagactgttt ttggatatgt gactgttgag ggcgtgaatc tcgaaggagt
     1261 gaatccgatt gcgaataccc tggtgacgtc gtttgtacag ggcgcgatca atcaacgggt
     1321 gaatccactc gtgctcgtca gtgaaccaca gctatcgttg atgatcccgg tcaaggccag
     1381 caacggcacc gtgaaagcac gcgcgacgga cgtgcgcgca gaggtgctgg atgggtcgtt
     1441 gaagctttat ttgacttatg aatttatggg cgtgaaaggc gaaggtactt agaacgcaga
     1501 ttaacgcaga ttacgcagat tcgcgtttat ctgcgtaatc tgcggctaaa agtctttgcc
     1561 tttcgaagtg gcccgcgatt tcttccgcgg ttttccgtcc gacgaagggc gtcaattccg
     1621 agacgcctgc gcgtgagatg cgctgaatcg aaccgaagtg tcgtaacagt ctccctttcc
     1681 gcttatcacc cacacccgga attgccgtca gctcagaagt aaaatcacgc aactcacgac
     1741 gtttccgatg ataagtaacc gcgaagcgat gcgtctcatc gcgaatcatt tgcatcagcc
     1801 gaagtatcag cgagtgagag tcgagataaa tcggttcgtc ttcacgatct ttcatcaaca
     1861 ggtacgcgac atcgttgtgt cgctgcggcg gcttgacgat ccccaccatc ggaatcgctt
     1921 cgagcccgag ctcgctcgtc gcattcgcgg cggcgtttag ttgcgctttg ccaccgtcga
     1981 tcatgatcaa atccggcagc ggctttcctt cttccagcaa cctgc
//
LOCUS       DASTEY010000332         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_304101, whole genome shotgun sequence.
ACCESSION   DASTEY010000332 DASTEY010000000
VERSION     DASTEY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304101"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFO72_05840"
     CDS             <1..836
                     /locus_tag="VFO72_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9478843.1"
                     /translation="SRVNAIIPPLSIDGPVLSIRRFGADPLKMAALVENKALTKDIAD
                     MLQMVVHARLNILISGGTGAGKTTLLNALSAFIPENERIVTIEDSAELQLQQPHVVRL
                     ETRPPNIEGKGEVTQRDLVKNALRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTTV
                     HANTPRDALARLETMIQMTGMRLSDRAMRQQVASALNLVVQVARMSDGSRRVTSISEI
                     TGMEGETITMQEIFLYERTGVDSQGQVLGRFRPTGIRPRFTERLKACGLQLPRVFFEE
                     M"
     gene            843..1808
                     /locus_tag="VFO72_05845"
     CDS             843..1808
                     /locus_tag="VFO72_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9478844.1"
                     /translation="MIVSFLVFVFALFLVLGAYLLATHGSDKKRARLQKRLSEALLHS
                     AHTEDIDVVLARNELMSEIPWVNRTMINIQATFHLKRMLDQADLHITPSRVLMFSFMA
                     GILGGLAASVLTVFIPLMILAGVVCAALPLAHVWWKRKKRFDAFLEQLPDALDLVSRA
                     LSAGHAFSEALHMVSEEMPEPIATEFRKAYEEQNLGLSVKLALENLTQRMPLLDLKMC
                     VTAVLIQRETGGNLAEILEKVAYTIRERFRILGDLKTLTTSSRLSAWLLCALPILVAL
                     AVTVLNPEYMSVLWKDPRGHYLIAAALFMQITGMLIVRKILKIQI"
     gene            1897..2838
                     /locus_tag="VFO72_05850"
     CDS             1897..2838
                     /locus_tag="VFO72_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9478845.1"
                     /translation="MIIIVALSTFVCISLALLGVYWLLYKPQSAATERLRRMGGGTEG
                     GNASGLHSVTIPDDNPAAELAQRLAEPLNKLLPPSATEAKKIQKKLMHAGFRSAESVI
                     IYRGIQLASMAGFPLLVAGVCALTARPLDSALLYIIAAFIVGFMLPRFFLNRTIRKRQ
                     RLIRWGLADALDLMVVSVEAGLGLNAAMVKVATELREVHYHVATEFELANLEIRVGRD
                     RDEALRNLSERTGVDDMKSFIAMLIQTDKFGTSIAKGLRTFSDGLRTKRRQRAEQEAQ
                     KAAVKLLFPLACFLFPTLFIAILGPAFLNLADVLDMM"
     gene            2851..3804
                     /locus_tag="VFO72_05855"
     CDS             2851..3804
                     /locus_tag="VFO72_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478846.1"
                     /translation="MNKRRILAVLIAFVLTIAATAMWSASTIQREGDPDEIVLEDSGS
                     EAGSVDQPKKKGGNKVVKVLAAPFKAFGRLFGGGDDKNKIHRMTEKDAERFESVAVTR
                     IEDARNQSVKKDTSSASAREHMEVGREYLVEGRYSEAISELSLAVSLDAKLTEAHNLL
                     GVAYDKKGFGDRAKDSFQRAVKLEEDADTLNNLGFSLYQSGNYRAAVDRLKRAAKLAP
                     TDERILNNLGLAYCRLGKIDEAYKAFARATGPLTGNLNTAKMLERFGREDDAIRYYEA
                     AREIDPINTIALRRLADLYQRVGRASESEAARNALQLASNK"
     gene            3844..>4071
                     /locus_tag="VFO72_05860"
     CDS             3844..>4071
                     /locus_tag="VFO72_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478847.1"
                     /translation="MSHTAILTRAEIESMGPPLPEETPDTGISEGFLCDLALKHVAML
                     PEPTTMAVAERINLPRMLTEDLLQKLYREKLI"
BASE COUNT          912 a         1097 c         1162 g          900 t
ORIGIN      
        1 gttcgcgtgt taacgcgatc atcccgccgt tatcgatcga tggccctgtg ctttcgatcc
       61 gtcgtttcgg cgctgatccg ctgaaaatgg cggcgctcgt cgaaaacaaa gcactgacca
      121 aagacatcgc agacatgctg caaatggttg tgcatgcgcg cttgaacatt ttgatctcgg
      181 gtggtaccgg cgctggtaaa accacgctgc tcaacgcgct ctcagcattc attcccgaga
      241 acgaacgtat cgtgacgatc gaagactcgg ccgaattgca actgcaacag ccgcacgtcg
      301 tcagactcga aacacgtccg cccaacattg aaggcaaagg cgaagtcacg cagcgagatc
      361 tcgtgaaaaa cgccctgcgt atgcgtcccg atcgcatcgt gatcggcgag gtccgcggtg
      421 gtgaagcgat cgacatgctt caggccatga acactggtca cgacggcagt ttgaccaccg
      481 ttcacgccaa cactccacgc gacgctctgg cacgactcga gacgatgatc cagatgaccg
      541 gcatgcggtt gtcggatcgt gccatgcgcc aacaggtcgc gtcggcgctc aacctggtag
      601 tgcaggtcgc gcgcatgtct gacggttcgc gtcgagtgac gtccatttct gaaatcaccg
      661 gcatggaagg cgagacgatt accatgcagg agatcttcct gtacgagagg acgggagttg
      721 attcacaggg tcaggtgctc ggacgtttcc gtccaaccgg tattcgccca cggttcaccg
      781 aacggttgaa ggcatgcggt ctacagttgc cgcgcgtctt tttcgaggag atgtaactca
      841 caatgattgt atcttttctc gtatttgtgt ttgcgctgtt cctggtcctg ggcgcttatc
      901 tgctggctac gcacgggtca gacaagaagc gtgcgcggct gcaaaaacgg ctgtcggagg
      961 cgctgcttca ttcggctcac accgaggata tcgacgtggt actcgctcgc aacgagttga
     1021 tgagcgagat cccgtgggtg aatcggacga tgatcaacat tcaggcgacg ttccacctga
     1081 agcgaatgtt ggaccaggcc gacctacaca tcacgccctc gcgagtactg atgttttcgt
     1141 tcatggcggg catccttggc gggttggctg catcagtctt aacagttttc attccgctga
     1201 tgatcctcgc cggtgttgtg tgtgcggctt tgccgctggc gcacgtttgg tggaagcgca
     1261 agaaacgttt cgatgctttt ctggaacaac tgcccgacgc actcgacctg gtcagcaggg
     1321 cgctgagcgc aggccatgcg ttctctgaag cgctgcacat ggtttcggaa gagatgccgg
     1381 agccgatcgc caccgagttt cgcaaagctt acgaagaaca aaaccttggt ctatcagtga
     1441 agctcgcgct cgagaacctg acgcagagaa tgccgttgct cgatctcaag atgtgcgtaa
     1501 cggcggttct gattcagcgt gagacgggcg gtaatctcgc cgagatcctc gagaaagttg
     1561 cttacaccat tcgcgagcga ttccggatcc ttggtgatct gaaaacgctg acgaccagtt
     1621 cccggctgtc ggcctggctg ctatgcgcct tgccgatctt agtcgcgttg gcagttacag
     1681 tgctgaatcc tgagtacatg agcgtgttgt ggaaggatcc acgcggtcac tatctgatcg
     1741 cggcggccct cttcatgcag atcacgggta tgttgattgt gagaaagatc ttgaagatac
     1801 agatctaatg gctttgtact ttggtctttg gtctttgtgc ctttgaacca agtccaagac
     1861 caaagaccaa agacccaaga cccaagaacg caaaccatga taatcatagt cgccctttca
     1921 actttcgtct gtatctcact cgcattgctg ggtgtttact ggttgctcta caaaccgcag
     1981 agtgcagcca cggaaagact gcgccgcatg ggtggtggaa ctgaaggcgg caatgcctca
     2041 ggcctgcatt cggtaactat acccgatgac aatcccgcgg cggagctggc gcaaaggctc
     2101 gcagagccct tgaacaagct gctgccacca tccgcgacgg aagccaaaaa gatacaaaag
     2161 aaactgatgc acgccggctt tcgttccgcg gaatccgtca tcatctatcg cgggattcag
     2221 ctggcctcga tggccggatt cccgttgctg gttgcgggtg tgtgcgcctt gaccgcgcga
     2281 ccgctggata gcgcgctgct ctatatcatc gccgctttta tcgtgggctt catgctgcca
     2341 cgcttctttt tgaaccgtac tatacggaaa cgccagcgac ttattcgctg gggcctcgct
     2401 gacgcgctcg acctgatggt tgtttccgtc gaggcaggtt tgggtttgaa cgcggcaatg
     2461 gtgaaagtcg ccacggaatt gagagaagta cactatcacg ttgcaactga attcgagctc
     2521 gccaatctgg aaatccgagt cggacgcgac cgtgacgaag cgttgcgcaa tctgtcagag
     2581 cgcacgggtg tcgacgacat gaagagtttc atcgcgatgc tgattcagac cgataagttt
     2641 ggtacttcga tcgcaaaagg actgcgcacg ttctcagatg gactgcgcac gaaacgtcgc
     2701 cagcgagccg aacaggaagc gcagaaagct gcggtcaaac tattgttccc gctggcttgt
     2761 tttctattcc cgacgctgtt tatcgcaatc cttggccctg ctttccttaa cctcgctgac
     2821 gttctcgaca tgatgtagag gagtggacca gtgaacaaac gtagaatctt agctgttcta
     2881 atcgcttttg tgttgaccat tgccgcaact gcgatgtgga gtgcttccac gatccagcgc
     2941 gagggagacc cggatgaaat cgtgcttgaa gattcgggtt ctgaggcagg cagcgtcgat
     3001 cagccgaaga aaaagggtgg aaacaaagta gtaaaggtgc tggcggctcc gttcaaggct
     3061 ttcggaagac tgtttggcgg tggtgacgat aaaaacaaga tccatcgcat gaccgagaag
     3121 gatgctgagc gctttgagag cgtcgctgtg accaggatcg aagatgcgcg taaccagtca
     3181 gtgaagaaag acacttcgtc tgcttcagcc cgtgagcata tggaagtcgg gcgcgagtat
     3241 ctggtcgaag gtcgttatag cgaagcgatt tcagagctgt cgctggctgt ttcgctcgat
     3301 gcgaagctga cggaagctca caacctgctg ggcgtcgcgt acgacaagaa gggctttggc
     3361 gatcgcgcca aggattcgtt ccagcgcgcc gtgaagctcg aggaagatgc tgacacgctg
     3421 aacaatcttg gtttttcgct gtatcagagt ggtaactatc gcgccgccgt cgatcgtttg
     3481 aagcgcgccg cgaaattggc gccgactgac gagcgcatcc tgaacaatct tggcctggcg
     3541 tattgccgcc tgggcaagat tgatgaagca tacaaagcat tcgcacgcgc caccggtccg
     3601 ttaaccggca atctcaacac cgccaagatg ctcgagcggt tcggtcgtga agacgatgct
     3661 attcgttact acgaagcagc acgcgagatc gatccgatca acacgatcgc gcttcggcgc
     3721 ctggcggatc tctatcagcg cgtaggccgc gcgagcgaat cagaagcagc tcgcaacgcg
     3781 ctccaacttg cttccaacaa ataggtccaa taggtcctat tctaagaaaa gagagaaact
     3841 cacatgtcgc acaccgcaat actgacgcgc gcggaaatcg agagcatggg cccgccgctg
     3901 cccgaggaaa caccggacac cggtatctcg gaaggctttc tttgcgatct cgcgttgaag
     3961 cacgtggcca tgctgcccga accgacgacg atggctgtcg cggaacgcat caatctgcca
     4021 cgcatgctga ccgaggatct gctccagaag ctttaccggg aaaagctgat c
//
LOCUS       DASTEY010000333         6778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1309359, whole genome shotgun sequence.
ACCESSION   DASTEY010000333 DASTEY010000000
VERSION     DASTEY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309359"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="VFO72_05865"
     CDS             <1..1911
                     /locus_tag="VFO72_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478848.1"
                     /translation="FPAVRFPGTVLWCNFELARELGFNVPRTNHLTPELHEELLDKLS
                     LRAVTPGDDVPDIEVVKMYADRYGGDGVSPALGAGRAGFLREGNLYIKGLGFTPLFRH
                     NDKDDFVHSHGGVHLEDCMSEAVFGEVNQNLFELGSSRLVAIIDQGMFVTEPSGRRRD
                     IALAIRTGTQLRPGHLLAKRVRGSRSPLEMFVSITRATGQLVFQNGSGTPDLGATMLS
                     IIDDHARTAAGSFRWRMIHGALSPSNMDVSGAMLDLPTQSTQPRTAPIFSLDYVHSSF
                     GTEHKERGFYLAEMYRRILRTTDAPTRERFNLKWLNVSNQMDSDYAKYLQLKLVGAAG
                     FKAEFATRICTENSDAAARFCQTVLTMAALKNAGSACVARKVVEDVCVVDVFNVLGNL
                     PAEYFNGDDLTESVLRHARPVFKGNRFHVRAKERKVEQLAAEFSGVYRELMNVASVYY
                     PSRSEMERSITSRAVFENQPLEALYCFPLYQELNAAIAEYKTTGNAQIISDAIDTRIT
                     RSLRSVDALLARGSSLRTIRGISYSVRAGKLNINIPVEARGDQFVTAVPGLDSLTKRQ
                     IRELRYRFTTDRAKTFHEARGRLLYDEQLGAIIDFGDLETVTLVGYLEGFFVTTDLRT
                     RTFSDYVFAIPD"
     gene            1911..2735
                     /locus_tag="VFO72_05870"
     CDS             1911..2735
                     /locus_tag="VFO72_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478849.1"
                     /translation="MWSIGIYKGHSPYHLSPAGNVPNLTGANVTDVTASFVADPFIVR
                     GNPWHMFFEVWNTDLNKGAVGLAQSHNGFDWKYEQIVLNEPFHLSYPYVFEWQNKYYM
                     IPETLGANAACLYQADDFPTRWSLAAKLIEGQCADPSIVRFADRWWLFICSTPYQHDT
                     LRLYFADELTGPWREHPKSPIIQNDPCRARPAGRMLVLDDKLIRFAQDCVPLYGTRVR
                     AFDILELTTSCYMEVENPASPVLQPSGTGWNALGMHHVDAHQQPDGSWLASVDGLG"
     gene            2869..3834
                     /locus_tag="VFO72_05875"
     CDS             2869..3834
                     /locus_tag="VFO72_05875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478850.1"
                     /translation="MRVLLSSEHQYPANGEVGSGLHPRALPSGSGFWMHDLVMKGLTE
                     LGHEVFYLLRNGAQGPMPPGAKVVSQPVWDADILHTISDRDRALVNEWQACGKPWVTT
                     CHMDMRTRGLAQPRTTENWIFVSRTLARLHGRTRYVLNGVDPEACIYSETKGDYVLFM
                     SSMDWEMQKGLDIALSLAARYRFRLVVAGTGNNYKRIARVATLCRQIGAHYVGDVRGK
                     KKAELLARAKAFLFPTRVDEAFGLGMAEALMSGTPVICSDKGACPELISPDVGFVCRS
                     DRDYMAALHNVSRISPQACREKAMREFHYQRMAADYVNEYEKELG"
     gene            complement(3909..4310)
                     /locus_tag="VFO72_05880"
     CDS             complement(3909..4310)
                     /locus_tag="VFO72_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HET9478851.1"
                     /translation="MVNKIRIKTDGAPPAIGTYSQAIRTGDLVFVTAQTGRDPDTGKL
                     EEGLEAQTRRMLSNLDAILNAAGCSPADIVKVTLLLADIKDFKAVDQIYSAWLPGLGI
                     TPLPARTAFAAASLPAGALVMMDVVAAFPLQ"
     gene            complement(4323..5093)
                     /locus_tag="VFO72_05885"
     CDS             complement(4323..5093)
                     /locus_tag="VFO72_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478852.1"
                     /translation="MVTSQAPRAFNREMYHDHPENVFYGTDDGAQDGSFSEFAEIKAH
                     YENVAPERHENIHMISVVGGLYGLNLIPLWKPTRLTIFDINPTAITYFKIVRTVFTTS
                     RDVAHFLQRLNDGDYDAHDEREEFVRENIRLKQRGALPRERGSTKRAYEQSWQHAFEH
                     FELTKKILTEAPLEIRTEPMESENFRSWIREQNNLWIYSSNITEFHYFDLEFADTSNV
                     VLLQIIYPGQVQLMDLAPLAGGPVRVNCEIPLRAERIV"
     gene            complement(5111..6289)
                     /locus_tag="VFO72_05890"
     CDS             complement(5111..6289)
                     /locus_tag="VFO72_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478853.1"
                     /translation="MRAAEAIAGLNNVETVTTDVSDEEELLKTAVQLRNQHGALYRIV
                     TAQETMLEAVAVANETLGLPAMSVETVRRTLDKSQFKSMLTSAGINTPRYQLVNNLAD
                     AQRFAAEIGFPIVLKSPAGSGALATLLVNNDVDLCRALQVAEHTLLAEQSVDGQELCI
                     DTITIAGEPQFHSVCCYYPSILEAVERPETQWACVMPRDMSSYGTFIEQGLQAIKALK
                     VGNAMTHMEGFIDSAGRPWFTDATLRPAGARIAPMLAYAYDIDPYRAWARVAVDEVFD
                     GPWERTYAAGTIFLRGVGDGLVRQVDGVEAVQAELRDLLVDVRWPRIGARKSATYTGD
                     GFITVRHHETEVVRKTLRRIAELVKITYSSNIQPPIETWQHRLRNFRELNRPAWDQAN
                     "
     gene            complement(6336..>6778)
                     /locus_tag="VFO72_05895"
     CDS             complement(6336..>6778)
                     /locus_tag="VFO72_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478854.1"
                     /translation="ESFAEPLRPRIGERSSWPEVSVEAQFIRHRAGYFLGPHSDLYTK
                     LVVLLLYLAPDDRAERLGTSLYRPKVDGFSCRDSKHYPFDDFIRVKTAPYRPNSLLAF
                     VRSDISFHGLEPLTEQDVATEGRDLIQYVVHDKAAREAQLRAKR"
BASE COUNT         1465 a         1902 c         1870 g         1541 t
ORIGIN      
        1 tttcccgcgg tgcgctttcc gggcacagtc ctctggtgca acttcgaact cgcgcgcgaa
       61 ctcggattca atgtgccgcg cacaaaccac ctcacgcccg agctgcacga agaacttctc
      121 gacaagcttt cgctccgcgc agtaactccc ggcgacgatg ttcccgatat cgaggtcgtg
      181 aagatgtatg ccgatcggta cggcggcgac ggagttagtc ccgcccttgg tgcaggccgc
      241 gcaggctttc tgcgtgaagg aaacctttat atcaagggcc tcggcttcac tcctcttttc
      301 cgtcacaacg acaaagacga tttcgttcac tcgcacggtg gtgttcatct cgaggactgc
      361 atgtcagaag ctgtctttgg cgaagtcaat caaaacctat tcgagctcgg ctccagccgc
      421 ttagttgcga tcatcgatca gggaatgttt gtcaccgaac cttccggccg ccgtcgtgac
      481 atcgcactcg ccattcgcac gggcacgcag cttcgaccgg gccatctgct cgcaaagcgt
      541 gttcgaggca gccgttcgcc gctcgagatg ttcgtgagta tcactcgagc gacgggccag
      601 ttggtgtttc agaatggatc cggcacgccg gatttgggcg cgacgatgtt gagtatcatc
      661 gacgatcacg cgcgcacggc tgccggctca tttcgctggc gtatgattca cggggcgctg
      721 agtccgtcga atatggacgt aagcggcgcg atgctcgact tgccgacgca gtcgactcag
      781 ccgcgcacgg cgccgatctt cagcctcgat tacgtgcact cgagttttgg cacggaacat
      841 aaggaacgtg gtttctatct ggccgagatg tatcgtcgca tactgcgcac gacagatgcg
      901 ccaactcgag agcgcttcaa cttgaagtgg cttaacgtct caaaccagat ggactcggac
      961 tacgccaaat accttcagct aaagttggtg ggcgcagcgg gttttaaggc ggagtttgca
     1021 acacggattt gcacagaaaa ctcggatgct gctgcacggt tctgtcagac cgtactcacc
     1081 atggcggctc taaaaaacgc cggttccgcg tgtgtggcgc gaaaagtggt cgaggatgtg
     1141 tgcgtcgtgg acgtcttcaa tgtgctcggt aatctcccgg cggaatattt taatggtgac
     1201 gatctgacag agagtgttct tagacatgcg cgtcccgtct tcaaaggaaa tcgtttccac
     1261 gtccgcgcta aggagcgcaa ggtcgagcaa cttgccgctg agttttccgg cgtctatcgt
     1321 gaactgatga acgtcgcgag cgtttactac cccagtcgat ccgagatgga gagatcgatt
     1381 acctcgcgcg cggtttttga gaatcaaccc ctcgaagcgc tttactgttt cccgctctac
     1441 caggagctaa acgccgccat cgccgaatac aaaacaacgg gcaatgcaca gatcatcagc
     1501 gatgcaatcg atacgcgaat cacgagatcg ttgcgcagcg tcgatgcgtt gttggctcgc
     1561 ggcagttcgc tgcgcacgat ccgagggatc tcgtattccg tccgagcggg gaaactaaac
     1621 atcaacattc cagtcgaggc acgaggtgat caattcgtga ccgccgttcc gggcctcgac
     1681 tcactaacga agcgtcagat ccgggaattg cgttatcgct tcacgaccga tcgcgccaag
     1741 acgttccacg aagcacgagg ccgcctgctc tacgacgaac aactcggcgc cataatcgat
     1801 ttcggcgatc tcgaaacggt gacgcttgtc ggttatctcg aaggtttctt cgtcacgacc
     1861 gatctgcgca cgcgcacttt cagcgactac gtgttcgcca tccccgacta atgtggtcca
     1921 tcggcatcta caaaggccac tcgccttatc atttgtcacc ggccgggaac gtgcctaatc
     1981 tcaccggcgc aaacgttacc gacgtaactg caagctttgt tgccgatccg ttcatagttc
     2041 gcggtaatcc gtggcacatg ttttttgaag tctggaacac ggatttgaat aaaggcgcgg
     2101 tcgggttggc gcagagtcac aacggatttg attggaaata tgagcagatc gtcctcaacg
     2161 aaccgtttca tctttcctat ccttacgtct tcgaatggca gaacaagtac tacatgattc
     2221 ccgagacact cggcgcgaac gctgcgtgcc tttatcaggc cgacgacttt cccacgcgct
     2281 ggtcactggc cgcaaaattg attgaaggac aatgcgccga cccatcgatt gttcgtttcg
     2341 ccgatcgctg gtggctgttt atctgctcca cgccatatca gcacgacacg ctgcgacttt
     2401 attttgctga cgaactcact ggcccatggc gggaacaccc gaagagtcca atcatccaaa
     2461 acgatccgtg tcgcgcgcgt cccgccggca gaatgctggt gctcgacgat aaattgatcc
     2521 gctttgcgca ggattgtgtt ccactgtacg ggacgcgtgt gagggctttt gacattctcg
     2581 aactcaccac aagttgttac atggaagtgg aaaaccctgc gagtcccgtc ctgcaaccgt
     2641 ccggcaccgg atggaatgcg ctgggcatgc atcacgtgga tgcgcaccag caacccgacg
     2701 gaagttggct cgcctccgtc gatggattgg ggtaattttt gatttttgat tttcgatttg
     2761 agttcggggc tggacgtcgt tactcgccgt ttttcaatcg caatcgaaaa tcggcaatcg
     2821 tcaatggcaa caatcaaaaa tcgaaaatca aaaatcaaaa ataaccccat gcgcgttctg
     2881 ctttcatctg aacaccagta cccggcgaac ggtgaagtcg gcagcggctt gcacccgcgc
     2941 gcgctgccgt ccggctccgg cttctggatg cacgatctgg tgatgaaagg actgaccgag
     3001 ttgggccatg aagtgttcta cctgttgcgg aatggcgcgc aaggaccaat gccaccggga
     3061 gcgaaagttg tttcacaacc agtttgggac gcagatatcc tgcacaccat ctccgatcgc
     3121 gacagagcgc tcgtgaacga atggcaggcg tgtggtaaac catgggtcac tacctgtcac
     3181 atggacatgc gcacacgcgg tctggcccaa ccacgaacaa ctgaaaactg gatcttcgtc
     3241 tcgcgtactc tcgctcgttt acacggccgc actcgatacg ttttgaatgg ggtcgatcct
     3301 gaggcctgca tctactctga aaccaaagga gactacgtgc tgttcatgtc gtcgatggat
     3361 tgggagatgc agaaggggct cgacatcgcg ctctcgctgg ctgcccgata tcgtttcaga
     3421 ctcgtcgtcg ccggcaccgg caacaactat aaacgcatcg ccagggtcgc gactctctgc
     3481 cggcagatcg gcgcgcatta cgtcggcgac gtgagaggca aaaagaaagc tgagttactc
     3541 gcccgcgcta aagcatttct cttcccgacg agagttgacg aagcgtttgg gttggggatg
     3601 gccgaagcgt tgatgtcagg cacgccggtg atctgtagcg acaagggagc gtgtccggag
     3661 ttgatctcgc ccgacgttgg cttcgtgtgc cgcagcgatc gcgattacat ggccgcactt
     3721 cacaacgtgt ctcgcatttc gccgcaggcg tgccgtgaga aagcgatgcg cgagtttcac
     3781 tatcaacgca tggccgcaga ctacgtcaac gagtatgaaa aggagctagg gtaggacagg
     3841 ctttcctgcc tgtcacggtt atcgcgcatc aagtgacagg caagaatgcc tgttctacga
     3901 aaaccagatc attgaagcgg aaacgccgcg acgacgtcca tcatgactag cgcgcctgcc
     3961 ggcagcgacg cggcagcgaa cgcggtgcgt gccggaagtg gtgtgattcc caaaccaggc
     4021 agccacgcgg aatagatctg atcgacggcc ttgaagtctt tgatgtcggc cagcagcaat
     4081 gtgactttca cgatgtcggc gggagagcag ccggcggcgt tgagtatcgc gtcgaggttc
     4141 gacaacatgc ggcgcgtttg tgcttcgagc ccctcttcga gttttcccgt gtcgggatcg
     4201 cggccggttt gggccgttac aaaaaccaga tcgccggtgc gaatagcctg tgagtaagtg
     4261 ccgatcgccg gcggcgcgcc atcggtctta atccgtatct tgttcaccat ctgatttgct
     4321 ccctaaacaa tgcgctccgc cctgagcggg atctcgcaat tgactctgac tggcccaccg
     4381 gcaagcggcg ccagatccat tagctgcacc tgtccggggt aaatgatctg caacagcacg
     4441 acgttcgacg tgtcggcaaa ttcaagatcg aagtaatgaa attccgtgat gttggagctg
     4501 tagatccaga gattgttctg ttcgcgaatc caggaacgga agttctcgct ttccattggc
     4561 tcagtgcgaa tttcgagcgg agcttcggtg agaatcttct tcgtcaattc gaaatgttcg
     4621 aaagcgtgtt gccagctctg ttcgtaagca cgcttggttg agccgcgttc gcgcggcaat
     4681 gcaccgcgtt gcttgaggcg aatgttctcg cgcacaaact cttcgcgttc atcgtgtgcg
     4741 tcgtagtcac catcgttgag ccgctgaaga aaatgggcga cgtcgcggct cgttgtaaac
     4801 acggtacgaa cgatcttgaa ataggtgatg gccgttggat tgatatcaaa gattgtgagg
     4861 cgcgttggtt tccacaatgg tatgagattg aggccgtata agccgccgac gacgctgatc
     4921 atgtgaatgt tctcgtgccg ttcgggcgca acgttttcgt agtgagcttt gatctcggca
     4981 aactcgctga acgagccgtc ctgggcgccg tcgtcagtgc cgtagaaaac gttttccgga
     5041 tgatcgtgat acatctcacg gttgaatgcg cgcggcgctt gtgaagtcac cattggtcgg
     5101 gtcctacctc tcaatttgcc tggtcccatg cgggccggtt gagctcgcgg aagtttcgta
     5161 gtctgtgttg ccacgtttct atgggcggct ggatgttgga actgtaagta atttttacta
     5221 attctgcgat ccggcgaagc gtctttcgaa ctacttctgt ctcgtgatga cggacagtga
     5281 tgaaaccatc gccggtgtaa gttgcggatt tgcgcgcgcc gatccgtggc cagcgtacgt
     5341 caaccaaaag atctcgcaat tcagcttgga cagcttccac accatccacc tgccgcacca
     5401 atccgtcgcc aacgccacgc agaaagatcg tgccggcggc ataggttcgc tcccacggtc
     5461 catcgaaaac ctcgtctact gcaactctgg cccaagcacg atacggatcg atgtcgtacg
     5521 catacgcaag catcggcgcg atccgcgcgc ctgcaggacg gagcgtggca tcggtgaacc
     5581 acggtcgtcc ggctgaatcg atgaatcctt ccatgtgcgt catcgcgttt cccaccttca
     5641 aggctttaat cgcctgcaac ccttgttcga taaaggtgcc gtaggaactc atatcgcgcg
     5701 gcatcacgca cgcccactgc gtctcaggtc gttcgactgc ttcgagtatc gacgggtaat
     5761 aacaacaaac tgaatggaac tgcggttcgc ctgcgatcgt gatcgtgtcg atgcacagtt
     5821 cctgaccatc gacactttgc tccgcgagca gcgtatgttc agccacttgt aaagcgcgac
     5881 aaagatcaac gtcgttgttg actagcaagg tcgctagcgc accgctgccg gccggagatt
     5941 taagcacgat tggaaaaccg atttctgccg cgaaccgttg cgcgtccgcc agattgttta
     6001 cgagttgata acgcggcgtg ttaatgcccg ccgaggtcaa cattgatttg aactgcgact
     6061 tatcgagcgt tcttcgcacc gtttcgacac tcatcgcggg caggcccaac gtttcgttcg
     6121 ccactgccac ggcctcgagc atcgtctctt gcgcagtgac gatgcgatac aaagctccgt
     6181 gttgatttcg aagctgaacg gcggtcttca gtaactcttc ttcgtcgctc acgtccgtgg
     6241 ttacagtctc cacgttattg agccccgcga ttgcctccgc ggctcgcaca ctttcactcg
     6301 acgccccacg acaaacaaac aatactcttc ttgtattaac gtttcgcacg caattgcgcc
     6361 tcgcgcgccg ccttgtcgtg gaccacatac tgaatcagat cgcgaccttc ggttgcgacg
     6421 tcctgctcag tcaatggttc gagtccatga aacgagatgt ccgaacgaac aaatgccaac
     6481 agtgaattcg gcctgtatgg cgcggtcttc acgcgaatga aatcgtcgaa cggatagtgt
     6541 ttgctgtcac gacaactgaa accgtcaact ttcggacgat agagcgaggt gcctaggcgt
     6601 tcagcacgat cgtcaggagc gagatacagc aacaggacaa cgagcttcgt gtagagatcc
     6661 gagtgtggtc caagaaaata tccggcgcgg tggcggatga actgcgcctc gacagacact
     6721 tccggccacg acgaccgttc gccgattcgc ggccgcagtg gttcagcaaa cgattcca
//
LOCUS       DASTEY010000334         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_913778, whole genome shotgun sequence.
ACCESSION   DASTEY010000334 DASTEY010000000
VERSION     DASTEY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913778"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2151
                     /locus_tag="VFO72_05900"
     CDS             <1..>2151
                     /locus_tag="VFO72_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9478855.1"
                     /translation="QLNFRSQPPLIAFFNHLFARLFQVPDDIPASEYKNLDQLGYVKH
                     EPSEPRRETHNDLKEGAPLVELLVTTKPSLEDDPRAEQDSRVLDAQQVARRILALKRS
                     SPDLKYGDIALLFRAMTQVQIYESVFRRANIPYQTVLGRGFYEREEITDLIQLLRFLD
                     NKTDEIALAAVLRSPLCGTSDNGLLALRCAPLLAETATADPLRGFAVTRRLYTALQRH
                     REIAYISDEEHELLDRAATLISELVARRHHYPIQDLLRFAVNESEYMAVIAANFDGAQ
                     RLANVERLFTLAARFESSGTHLIRDFVRYVEEFEAIGSRESEGQIDQAADAVRLMTIH
                     QAKGLEFPVVIIPDLQRLSRVGTDNWVLLDRHRGLTLKVPDGRGSLVAGCTYTNFEKR
                     YAWREQFESMRLLYVAASRAEDRLIMSGVTDQLASLNRANDCWLKWIWQALDLGERSA
                     GGVVELDDDVHLQLTINDVGQALLHVADSKAAVADSEAAVADSEAADKVESDRLESPS
                     YSLSEAFPLTRQIEKPIGNAIYRFSVTQLINYHRCPRQYYFDRVLQLPSSDELAVWNN
                     ADAPEPPANLTATLKGAVIHRFCERYTADQDAEACLRETFTEVMRLRQAQLADRLVEI
                     NFDAAIKDLLPLAKNYLASDVFKRVEAAPKIDNSVPPKDQTGLWSELSFRLRRPPGIL
                     YGVIDKLLITNAGIEIIDFKTNRIRPAKTEEPQP"
BASE COUNT          474 a          596 c          622 g          459 t
ORIGIN      
        1 tccagttgaa cttccgcagc cagccgccac taattgcgtt cttcaatcac ctgttcgcgc
       61 gactgtttca ggtgcccgac gacattccag cgagcgaata caagaacctg gaccagttgg
      121 gttacgtgaa gcacgagccg agtgaaccgc gccgtgaaac gcacaacgac ttgaaagaag
      181 gcgctccgct ggttgagttg ctggttacca ccaagccttc tctcgaagac gatccgaggg
      241 ccgaacagga ctcccgcgtg ctcgacgcgc agcaggttgc gcggcgcatc ctcgcgttga
      301 aacgttcgtc cccggatctc aagtacggcg acatcgcgct gctgtttcgc gcgatgactc
      361 aggtgcagat ttacgagtca gttttccggc gcgcaaacat tccttaccaa accgtgcttg
      421 gtcgcggttt ttacgagcgc gaagagatca ctgacctgat tcaactgctc cgcttcctcg
      481 acaacaaaac cgatgagatc gcgctcgccg ctgtgcttcg ttcgccgctt tgcggcacct
      541 ctgacaacgg cttgctcgca ctgcgttgtg cgccactgct tgcagagacc gcgactgcgg
      601 atccgttgcg cggctttgcc gtaacgcgcc ggctttacac ggcgttgcaa cgtcatcgag
      661 agatcgctta catcagcgat gaggaacacg agctgctcga tcgcgctgcg acactgatca
      721 gcgaattggt tgcgcgacgg caccactatc cgatacagga cctgttgcgt tttgcggtta
      781 acgaatccga gtacatggcg gttatcgccg ccaacttcga cggcgcgcag cggcttgcaa
      841 acgttgagag gttgttcacg ctggcggcgc ggtttgagag ttcgggcaca cacttgattc
      901 gcgactttgt gcgttacgtc gaggaatttg aagcaatcgg cagtcgcgaa agcgagggac
      961 agatcgatca ggcggcggac gcggtgcggt tgatgacgat tcaccaggcg aagggtctcg
     1021 agtttccggt cgtgatcatt cccgatttgc aacgcctgtc gagagtgggt acggacaact
     1081 gggtcttact cgatcgtcat cggggcctga ctttgaaagt gcctgacggc cgcgggagtc
     1141 ttgtcgctgg ttgcacctac actaacttcg aaaagcggta cgcgtggcgc gaacagtttg
     1201 aaagtatgcg actgctttat gtcgcggcgt cgcgcgccga agatcgcttg atcatgtccg
     1261 gcgtcacgga ccagctcgcc tcgctaaaca gggcgaatga ttgctggctc aaatggatct
     1321 ggcaagcgtt ggatctgggg gagcgatcgg ccggtggtgt agtcgaactg gatgatgacg
     1381 tgcacttgca attgacgatc aacgacgtag gacaggcact cttgcatgtc gcagatagca
     1441 aagcagctgt cgccgatagc gaagcagctg tcgctgatag cgaagcagcc gataaggttg
     1501 aaagtgacag actggaaagt ccgtcctact cgctgagcga ggcgtttcca ttaacgcgcc
     1561 agatcgagaa gccgataggc aatgcgatct atcgtttcag cgtaacgcag ttgattaatt
     1621 atcaccgctg tccacgacaa tattacttcg atcgcgtgct gcaactgccc tcgagcgacg
     1681 aactcgcggt ttggaacaat gctgatgccc ctgagccgcc cgcaaatttg acggcgactt
     1741 tgaaaggcgc agtgattcat cgtttctgcg aacgctacac ggcagaccaa gacgccgaag
     1801 catgtttacg cgaaacgttt acggaggtga tgcgcttgcg acaagcgcag ctcgcagatc
     1861 gactcgtcga gattaacttc gacgcagcca tcaaagatct gttgccgctc gcaaagaact
     1921 acctcgcgag cgacgtgttc aagcgcgtcg aagccgcgcc gaagatagac aactccgtac
     1981 cgccgaagga tcaaactggt ctgtggagcg agttgagttt tcgtttgcgg cgcccgcccg
     2041 gcattctgta tggcgttatc gataaacttt tgatcacgaa cgcgggtatc gaaattattg
     2101 acttcaagac caaccgcatc aggcccgcaa aaaccgagga gccgcaaccc g
//
LOCUS       DASTEY010000335         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_419361, whole genome shotgun sequence.
ACCESSION   DASTEY010000335 DASTEY010000000
VERSION     DASTEY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419361"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>2069)
                     /locus_tag="VFO72_05905"
     CDS             complement(139..>2069)
                     /locus_tag="VFO72_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478856.1"
                     /translation="KITAAGAGSLLHDAAELDVTALAIGRDGSLFAGTSPDGKVYRIT
                     ADGKADVYFDPGDKYIWSLAVMSDGSLAVGTGDTGKLYRVRAAGATAESSLLVSTNQT
                     HVISLAVTPQGDLIAGTDSGGLVLRVSPEGKTFALFDTQLREIHALAPAADGSIYALA
                     LSDAAAGARVPSTPVVQPQPTEGATPTTSVTITALDETGAPIQGQLGQTRSRSDVSNA
                     RSAVFRILPDGATDVVWSSPTVTAFAIAPALQPGSVLIGTADKGRIYSVTNDGRDTLL
                     LQSPEGQISSLLVRNNQIYAASSNQGKLFRFGNELVSEGTFESPVRDAKLTASWGRIW
                     WRGGGAIEVQTRTGNGERPDATWSDWSTVYRDPEGNQISSPRARFIQWRATVRASASP
                     NSPSWVEDVSVAYLPRNVAPEVLSITALPIGVGLQQIAQVVVDPNVESSGLDPSVFGP
                     VAQVPPRRFYQRGARSFQWQAEDRNADTLEYAIYYRALNDQTFRLLKDKLRDNFYTID
                     GATLADGRYVIKVVATDAPDNPPGQKLTAERLSEPVDIDNTPPVVKVTGQPQVKGDSV
                     RVVFAADDATGKVKKADASLDGAAWTPVFPDDGIADSGHEVYSVEFGSLAPGEHTISL
                     RTFDSSGNIGTLSVTVRR"
BASE COUNT          429 a          626 c          583 g          431 t
ORIGIN      
        1 taggccacaa aaaggcacaa aagtcacaaa agagaaaagc ccttaacgcc tcatcagtga
       61 aaacccccaa cgctctataa gagaaagcct cagcgtttca tatcagaaac tattgtgtgc
      121 cttttgcggc aaatcctttt agcgacggac ggtaacgcta agggtgccga tgttgccact
      181 gctatcaaaa gtgcgcaagg agattgtgtg ctcgccgggt gccagcgaac cgaattccac
      241 ggaataaact tcgtggccgc tgtctgcgat gccatcgtcc ggaaagactg gcgtccacgc
      301 ggcgccgtcg aggctggcat cagccttttt gactttgccg gtcgcatcat cggctgcaaa
      361 aactacccgc accgagtctc cctttacctg cggctgaccc gtcaccttca ctaccggcgg
      421 cgtgttatcg atgtcgactg gttcactcaa tctctcggcc gttaacttct gacccggcgg
      481 gttgtcgggc gcgtccgtgg caacgacttt gatgacataa cgcccgtcgg cgagtgtagc
      541 gccgtcgatc gtgtagaagt tatcccgcag cttgtctttc agcagtcgaa acgtttggtc
      601 gttcaaagcg cggtagtaga tcgcatactc gagcgtgtcg gcattgcggt cttcggcctg
      661 ccactgaaac gatcgcgcgc cgcgctggta aaaacgtctc ggcggcacct gggccactgg
      721 accaaacacc gacggatcca accccgacga ctccacattc ggatctacca ccacctgcgc
      781 aatctgctgc aaccccactc cgatcggtag cgccgtgatc gacagcactt caggtgcaac
      841 attgcgcggc aaataagcga cactcacgtc ttcaacccac gacggcgagt tcggcgatgc
      901 cgaagcacga actgtagcgc gccattgaat gaagcgagca cgcgggctcg agatctggtt
      961 gccttcagga tctcgataaa cagtgctcca atcgctccac gtcgcgtcgg gccgttcacc
     1021 attacccgtg cgcgtttgaa cctcgatggc gccgccgccg cgccaccaga tccggcccca
     1081 cgacgccgtt aactttgcgt ctctgaccgg tgactcaaat gttccttcgc tcaccagctc
     1141 gttcccaaaa cgaaagagct ttccctgatt gctggaggcc gcgtatatct ggttgttcct
     1201 gaccagcaac gacgagatct gtccttcggg actttgcaac agcagcgtgt cgcgaccgtc
     1261 attcgtaaca gagtagatcc gtcccttgtc agccgtaccg atgagtacgc tgccgggttg
     1321 caatgcggga gcgatagcga aagcagttac ggttggagaa ctccacacca catcagtagc
     1381 gccgtcggga agtattcgaa acaccgcgct gcgtgcgttc gagacgtcgc tacgactgcg
     1441 tgtttggcct aactgtcctt gaatcggcgc gcctgtctcg tcgagcgctg tgatggtgac
     1501 ggaagtagtc ggcgtggcac cttctgtggg ttggggctgc accactggtg ttgacggcac
     1561 gcgggcgccc gccgctgcat cgctcagtgc cagagcgtag atcgatccgt cagccgccgg
     1621 cgcgagcgca tggatttctc gaagctgtgt gtcgaagagt gcgaacgtct ttccttcagg
     1681 ggatactctc agcacgagtc ctccagaatc cgtgccggcg atgagatcgc cctgcggagt
     1741 taccgcgagc gagatcacgt gtgtttgatt ggtgctgacc agcagtgagg attccgcagt
     1801 ggcgcccgcc gcgcgcactc gatagagctt gccggtatcg cctgtgccaa cagccagtga
     1861 gccatcagac ataaccgcta aagaccagat gtacttgtcg cccggatcga aatagacgtc
     1921 agccttgccg tcagcagtga ttcgatagac cttgccgtcc ggagatgtgc cggcgaaaag
     1981 cgatccatcg cgaccaatcg cgagcgccgt tacatcgagc tcggcggcgt cgtggagcag
     2041 tgagcctgcg cccgcggctg taatcttgt
//
LOCUS       DASTEY010000336         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_320287, whole genome shotgun sequence.
ACCESSION   DASTEY010000336 DASTEY010000000
VERSION     DASTEY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320287"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFO72_05910"
     CDS             <1..710
                     /locus_tag="VFO72_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478857.1"
                     /translation="KPKVNRKQADEFRKWTRQAKQLSEARLRKDPKDVEALYFLGAAQ
                     GLEAAFAGGVERKFMAAFRAGKDSVEHHRDVLKLAPDFHDAELTIGLMNYVIGSLPLP
                     TKMLVATMGVRGSKKRGLQELERVAVEGKWARDVAHVLLVDLYKREKRWKDAIKTARL
                     LSEKYPRNYLFKLQIADALTSQIVALKQAKTPVAAEEKELQDLFASLTRDKTLDSATR
                     DLVQHRWTIARQQLESR"
     gene            742..1935
                     /locus_tag="VFO72_05915"
     CDS             742..1935
                     /locus_tag="VFO72_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9478858.1"
                     /translation="MTDHHSEELLDRIKEFVHTHVYPLEPDFLHRPFRELIPALNAKR
                     DEVKRMGLWAPHLPKEHGGLGLALSEFALVSEELGRTPIGHYLFNCQAPDIGNMEILL
                     THGTPEQKQTYFEPLARGDVRSCFSMTEPEHPGSNPTWMSTTAVKDGDDYVINGHKWF
                     TSSAEGASFAIVMAITNPEASNRYKRASQIIVPIPTPGFQLIRNISVMGDEGSDYASH
                     AEVRYENCRVPQSNLLGQEGEGFTIAQERLGPGRIHHCMRWIGICERAFDLMCSYASR
                     RELSPGVLLGSRQNVQEWIAESRAEINAARLLVLQTACAIDRQGAPAVRKDISLIKFF
                     VAGILQKVLDRAIQVHGALGLTDATLLSYWYRHERAARIYDGPDEVHKTVVARSELKK
                     YEQHD"
     gene            1919..>2116
                     /locus_tag="VFO72_05920"
     CDS             1919..>2116
                     /locus_tag="VFO72_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478859.1"
                     /translation="MSSTIDRSAPVRAGEELPHNLLRDYLAAHLPELDGPLTVEQFPA
                     GFSNLTYLLRIGDHELVLRRPP"
BASE COUNT          471 a          618 c          629 g          398 t
ORIGIN      
        1 ccaaaccaaa agtaaatcgt aagcaggcgg atgaatttcg caaatggacg cgccaggcaa
       61 aacagctttc ggaagcgcgg ctgcgtaaag atcctaagga cgtagaagcg ctttactttc
      121 tcggcgcggc tcaaggactc gaagctgctt ttgcgggcgg tgtggaacgg aagttcatgg
      181 ccgcctttcg cgcggggaaa gactcagtgg aacaccatcg cgacgtcctc aagctggcgc
      241 cggattttca cgacgcggaa ctgaccatcg ggttgatgaa ctacgttatc ggttcgctgc
      301 cgttgcctac gaagatgctc gtggcgacga tgggagtgcg cggctcgaag aaacgcgggc
      361 tgcaagagct cgaacgtgtc gcagtcgagg gaaagtgggc gcgtgacgtc gcgcacgtgc
      421 tgctggtcga tctctacaaa cgcgagaagc gctggaaaga tgcaatcaag actgcccggc
      481 tgctgtcgga aaagtatccg cgcaattatc tattcaagct ccaaatagct gacgccctca
      541 cgtcgcagat cgtcgcgctg aagcaagcga agacgcctgt cgccgcggaa gagaaggagt
      601 tgcaagacct cttcgcttcg ctcacgcgcg acaaaacgct cgactccgcc acgcgagacc
      661 tcgtccagca ccgttggacc atcgcccgtc aacaactcga atcgcgttga caatataggt
      721 cctataggtc atataggacc tatgaccgat caccacagtg aagaactgct cgaccggatc
      781 aaagaattcg tgcacacgca cgtctacccg ctcgagcccg acttccttca tcgcccgttt
      841 cgagagctca tccctgcgct gaacgcgaag cgcgacgaag tcaaacgcat gggtctctgg
      901 gcgccacacc taccgaaaga acacggcggc ctgggccttg cgctgtcgga gtttgcgctt
      961 gtcagcgaag agctgggccg cacgccgatc ggtcactacc tgttcaactg ccaggcgcct
     1021 gacatcggga atatggaaat cctgttgacg cacggcacac cggaacagaa gcaaacgtat
     1081 ttcgagccgc tcgcgcgtgg cgacgtgcgt agttgttttt cgatgactga gcccgagcat
     1141 cccggctcaa acccaacctg gatgagcacg accgcagtga aagacggcga cgattacgtc
     1201 atcaacggtc acaagtggtt tacgtcgtca gcggaaggag cgagcttcgc gatcgtgatg
     1261 gccatcacca accccgaagc gtcgaaccgc tacaagcgtg ccagccagat catcgtgccg
     1321 ataccaacgc cgggctttca gttgatacgc aacatcagcg tcatgggcga cgagggcagc
     1381 gactacgcca gtcacgctga agtccgttat gaaaactgtc gcgtgccgca aagcaacctg
     1441 ctcggccagg aaggcgaggg tttcacgatc gcgcaggagc ggcttggacc aggacgcatt
     1501 catcactgca tgcgttggat cgggatttgc gagcgtgcgt ttgatttgat gtgcagctac
     1561 gcctcgcgcc gagagctgtc gccgggcgtg ttgctgggca gcaggcagaa cgtgcaggag
     1621 tggatcgccg agagtcgcgc cgagatcaac gcggcgcgcc tgcttgtttt gcagacagcg
     1681 tgcgcgatcg atcgacaagg cgcgccggca gtgagaaaag atatttcgct gatcaagttt
     1741 ttcgttgccg gtattttgca gaaggtgctc gatcgcgcga tccaggtgca tggtgcgttg
     1801 gggctgactg acgccacgct cttgtcgtac tggtaccggc acgagcgcgc cgcgcgcatc
     1861 tacgatggac ccgacgaggt ccacaaaact gttgtcgcgc gtagtgagtt aaagaaatat
     1921 gagcagcacg attgatcgca gcgccccggt gcgcgcgggt gaagagctac ctcacaatct
     1981 cttgcgcgac tatctcgccg cacatctccc ggaactcgac ggcccactca cggtcgaaca
     2041 gtttccagcc ggcttttcca atctcacgta cctgctgcgc atcggcgatc acgagctcgt
     2101 gctgcgccgg ccgccc
//
LOCUS       DASTEY010000337         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_518397, whole genome shotgun sequence.
ACCESSION   DASTEY010000337 DASTEY010000000
VERSION     DASTEY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518397"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFO72_05925"
     CDS             <1..390
                     /locus_tag="VFO72_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478860.1"
                     /translation="MDIVWFAVAYSVLTVTFFRRHLLIERESRRIIFGISIALFVAAV
                     VASVFTYSTPTLFPSLMNPLVSLSLFLLMRKLFVRWKHREPIDTFFDWRPGLAADRLF
                     NVLYFTLGTFLMSLLYLVADLTGYPWH"
     gene            544..1473
                     /gene="rapZ"
                     /locus_tag="VFO72_05930"
     CDS             544..1473
                     /gene="rapZ"
                     /locus_tag="VFO72_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET9478861.1"
                     /translation="MARKTPNSPPAENNNVPDIVIITGLSGSGMSSATNAFQDLGYFC
                     VDNLPVTMLPTFGRLVTTDDDEKGISRAALVIDIREGRFLGDFEKQLKALRASGLRVT
                     VLFFEASDDVLQYRFSETRRPHPADRGDRLLAAIRAERKAMARIRSHADQIIDTSDHT
                     VHTLRSFLLARFSPDRKGAPMRVEVMSFGHKYGNPGDLELLFDVRHLPNPHFVPELKP
                     LSGHDPRVVRYLRSQDDVQETLQRFTDLLSYLLPLYRREGKSYVTVGIGCTGGRHRSV
                     MIANALTRALRREGFDARATHRDVRKANRTRAR"
     gene            1631..2077
                     /locus_tag="VFO72_05935"
     CDS             1631..2077
                     /locus_tag="VFO72_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET9478862.1"
                     /translation="MSGNPVETKRVAGVIVTHGHLAGEFLAAAEMIIGPISHIAAVSI
                     GWHDDVDAAHDEVQRAITRVSQGVGVLLLTDMFGGTPTNIASMFLEEGSVEVVTGVNL
                     PMVIKLASFSTEEPLAEVARKICEQGRQGIYFAGGLLAPPTKSGAD"
BASE COUNT          455 a          620 c          628 g          494 t
ORIGIN      
        1 atggatatcg tgtggtttgc ggtagcatat tcggttctaa ccgttacgtt ctttcgccgt
       61 catctactga tcgaacgcga aagccgcagg attatcttcg gcatttcaat cgctttgttt
      121 gtggcggcag tagtcgccag cgttttcacg tacagcacac cgacgctgtt tccgtcgttg
      181 atgaatccgc tggtttcact cagcctgttt ttgttaatgc gcaaattatt cgtccgctgg
      241 aaacaccgcg agccgattga cacgttcttc gactggaggc cagggctggc cgcagatcga
      301 ttgttcaatg tcctgtactt taccctgggc acgttcttga tgtccctgct gtaccttgtg
      361 gcggatctaa ccggctaccc atggcattga acggctgcgg ttcgcagcga catgtggata
      421 cttggcgata tctacgcttg acgatcgagg tcatgcttcg ttggccttcg aaaccagtaa
      481 tcccaagtga atatccccga atgacaacaa aaaatgtcag tggtataagt gtctctcacc
      541 gagatggccc gtaaaactcc aaactccccg cccgctgaaa acaacaacgt tcctgacatt
      601 gtaatcatca ccggtctgag cgggtcgggc atgtcctccg ccaccaacgc gtttcaggat
      661 ctgggctatt tttgcgtcga caatctgcca gtaacaatgc tcccgacgtt tggccggctg
      721 gtcaccaccg acgacgacga aaagggcatc agccgcgccg cgcttgtcat tgacattcgc
      781 gagggcaggt tcctcggcga tttcgaaaaa caactcaagg cgttgcgcgc ttcaggactg
      841 agagtgacgg tcctcttttt tgaagcatcc gacgacgtgc tccagtatcg cttcagcgag
      901 acacgacggc ctcatcccgc cgatcgaggc gaccgtttgc tggcggctat ccgcgcggag
      961 cggaaggcca tggcccggat tcgatcgcac gccgatcaga tcatcgacac ttccgatcac
     1021 acagttcaca ccttgcgtag ttttctgctg gcgcggttca gtcccgaccg caaaggtgcg
     1081 ccgatgcgcg tcgaggtgat gagtttcggc cacaagtatg gcaacccggg ggatctggag
     1141 ttattgtttg acgtgaggca tttgccgaat ccccatttcg tgcctgaatt gaagccgttg
     1201 tcaggtcacg atccgcgtgt cgttcgatat ttacggtcgc aggacgacgt gcaggagacg
     1261 ctgcaacgtt ttacggatct gctgtcgtac ctgctgccgc tctacagacg tgaggggaag
     1321 tcgtatgtga cagtcggcat cggttgcacc ggcgggcggc atcgctccgt gatgatcgca
     1381 aacgccctga cgcgcgcact gcgtcgcgaa ggattcgacg ctcgcgccac gcatcgcgac
     1441 gtgcgcaagg cgaaccgcac acgtgcgcgg tgaagatgtc gaaggcagga acagggtctt
     1501 ccgcaaaggc tcaaaggcgc aaagaaggaa ctgtgaatca gatagctatg agtgacattc
     1561 tttacagctt gtctttgcgc ctttgcgcct ttgcgggaga actcttgagt cgcttacaga
     1621 ggaaacacgg atgagtggta atcccgttga aaccaaaaga gtcgcaggcg tcatcgtcac
     1681 gcacggccat cttgcgggcg aatttcttgc cgctgccgag atgatcattg gtccaatctc
     1741 gcacatcgcc gcggtctcga tcggctggca cgacgacgtc gacgctgcac acgatgaagt
     1801 gcagcgggcc atcacgcgcg tctcgcaagg cgtaggcgtg ctcctgctga ccgacatgtt
     1861 cggcggcacg cccaccaaca tcgcatcgat gtttttggaa gaagggtcgg tggaagtcgt
     1921 gactggagtg aacctgccga tggtgatcaa gctcgcgtcg ttttcgactg aagaaccact
     1981 cgcggaggtc gcgcgcaaga tttgcgaaca gggccggcag ggaatttact tcgccggcgg
     2041 attacttgct ccgccaacga aatccggagc cgattgagag tggctgaacg cagactccag
     2101 gtcacgggcc gactcggtct acacgctcgc gctgccgcca acctcgtgcg cgtggcgggc
     2161 cgattccaaa gcagcctcac gattcatcgt gtcgacg
//
LOCUS       DASTEY010000338         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1342117, whole genome shotgun sequence.
ACCESSION   DASTEY010000338 DASTEY010000000
VERSION     DASTEY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342117"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFO72_05940"
     CDS             complement(<1..272)
                     /locus_tag="VFO72_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478863.1"
                     /translation="MRRLPLLFLLAAISALDIAAQSGRRITTPATTAPPPPVQPPLNP
                     VPEVRSGPRPLTELMFLPESLRDRPIKAIRGDNFRLSDFEGKIVVI"
     gene            complement(320..1231)
                     /locus_tag="VFO72_05945"
     CDS             complement(320..1231)
                     /locus_tag="VFO72_05945"
                     /inference="COORDINATES: protein motif:HMM:NF006159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9478864.1"
                     /translation="MTSLKGKVALVAGATRGAGRGIARELGAAGAIVYCTGRSTKQQR
                     SEMDRPETIDETAELIEHAGGRAVAVQVDHLDPSQVEALVKRIERDHGRLDVLVNDIW
                     GGDHFIQWNVPVWEHSLENGLRMLRLAIDTHIITSHFALRLLIKNPGGLVIEVTDGTA
                     EYNSDHYRVSLFYDLAKTSVVRMAWLLAQELKPHQCTAVALSPGWLRSEQMLDNYGAT
                     EENWRDATKVQPHFIISETPRYVGRAVAHLAADPNAARWNGQSLSSGQLAKVYGFTDL
                     DGSQPDAWRYLVEVQDPGKPADATGYR"
     gene            complement(1256..2605)
                     /locus_tag="VFO72_05950"
     CDS             complement(1256..2605)
                     /locus_tag="VFO72_05950"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9478865.1"
                     /translation="MLLIEKDARAICEKLLSYVKADDAVVGVTSENYGHLRFAANAFT
                     TSGARENLSVAVTVWIDRKRGAASTNEIDDESLKATVALAEQLARLSPVDREYMPTLS
                     KQTYKPTNPFAEATSNISVAARAKTINDVISACEKAKVVGAGFHQARGSASASATKNG
                     NFVYERSSLVSLATTARTPDGTSSGYFLRNHFDVAKLDTSRVAREAIRKALDARSPRA
                     LDPGVYPAILEPQAVADLLVAFAFSFDARNAEEGRSAFSAPGGKTKLGEKIFDERINI
                     YSDPWHPELPASQSAQGGLPAQKIYLVRNGVLENLVYSRFWAKQKGKEPTPGPVNNIM
                     ESSQPAVSVEEMIKSMDRGLLLGRFWYIRGVDPRIVLQTGLTRDGIWYIENGKIQYPV
                     KNFRFNQSVIELLAPGNVEMIGKSERVGSSEGQGGNAALLPALKVKKFHFTSQSEAV"
     gene            complement(2797..4380)
                     /locus_tag="VFO72_05955"
     CDS             complement(2797..4380)
                     /locus_tag="VFO72_05955"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET9478866.1"
                     /translation="MTTSRRDFLKTAGAAAGALSLSTLPEWFRSVVAAEEAAANRIDK
                     NALADVALSTARKLGVTYADIRINRYRNESIATREQQVQNVSRSQNFGFGVRVLYKGT
                     WGFAASRSLTPEDVRRITQQAVEIARANSAYQRKRVSLVPVEKIVTSWKSAFEKDPFE
                     VSIDDKIQFLLSVNESAMKTSGVSFVSSSMGFVNEQKFYASTDGSRIEQYIIRANPNF
                     NVTAVNRSNGDFQSRSALGGPQGMGYEYMEKHDWKREAQQAGEEAVMKLKAKPVEAGK
                     YDLVLHPSHLWLTIHESVGHPTELDRALWMEANYAGTSFITPDKTGKLQFGSKIVHFV
                     ADRTQPAGLATVGYDDEGVRAQSWHLIKNGVFIDWQTTRDLAAMTGRKKSYGCNHGDS
                     WGSISFPRMPNISLQPGPDNTSLDDLISGVEKGILIYGDGSYSIDQQRYNFQFGGQVF
                     WEIKNGKRGDMLRDVAYQSRTTDFWNSCDGLGGQATYELGGSFNDGKGEPGQSNAVSH
                     GCPVARFRQTNVLNTARRS"
BASE COUNT          925 a         1254 c         1294 g         1070 t
ORIGIN      
        1 ttgatcacga cgatcttgcc ctcgaagtct gagagacgga agttatcgcc gcggattgct
       61 ttgatcgggc gatcccgcaa actctccggc aaaaacatca gctcggtaag tggccgcggt
      121 ccggatctaa cttcgggcac gggattgagt ggtggttgaa ccggtggcgg cggagccgtc
      181 gtagccggag tagtaattcg ccttccggat tgagccgcaa tgtccaatgc cgaaatagca
      241 gcgaggagga agagtagggg aagtcttctc atgatgagtt gagagaataa ccgatttttg
      301 gttgtttgag cagtcgggat tagcgatatc cggttgcgtc tgccggcttg ccgggatcct
      361 gaacttcaac gaggtaacgc cacgcgtcag gttgtgagcc gtccagatcg gtaaagccgt
      421 agaccttcgc caactgtcca ctcgaaagtg actggccatt ccagcgcgca gcgtttgggt
      481 ctgcggcgag gtgcgcaact gcgcgaccaa cgtaacgcgg tgtttcactg atgatgaagt
      541 gcggttgtac cttcgttgcg tcgcgccagt tttcctcggt agccccgtag ttgtcgagca
      601 tttgctcgga gcgcagccag cctggactca atgctactgc cgtgcactga tgtggtttga
      661 gttcctgggc caacaaccac gccatgcgaa cgacggaagt cttcgcgaga tcgtagaaaa
      721 gcgagacgcg gtaatgatcg ctgttgtatt cggcggtgcc gtctgtaact tcgatgacta
      781 atcctcctgg gttcttgatg agtagcctga gtgcgaagtg actagtgatg atgtgagtat
      841 caatcgcgag acgaagcatg cgcaggccat tttcaagcga gtgttcccaa acgggaacgt
      901 tccattgaat gaagtgatcg ccgccccaga tgtcgttgac cagcacatcg agccgcccgt
      961 gatcacgttc tatacgtttc acgagagctt ctacctggga cgggtcgaga tgatcgactt
     1021 gtaccgcaac ggcgcgtccg ccggcgtgct cgatcagttc ggcagtctca tcgatcgtct
     1081 cgggacggtc catctctgag cgctgttgtt ttgtcgaacg tcccgtgcaa taaacaattg
     1141 cacccgccgc gccaagctcg cgcgcaatcc cacgacccgc cccacgcgtc gcacccgcga
     1201 cgagcgccac ttttccttta agacttgtca ttaattaatc cgtcttatcc gtggcctaaa
     1261 cagcttccga ttgcgaggtg aagtgaaact tctttacctt caacgcgggc aatagcgcag
     1321 cgttgccgcc ctggccttcc gaacttccga cacgttcgga tttgccgatc atctccacgt
     1381 tgccgggtgc gagaagttcg atcacgctct ggttgaagcg gaagttcttg acaggatact
     1441 gaatcttgcc gttttcgata taccagatgc cgtcgcgcgt gagtccggtc tgcagcacga
     1501 ttcgcggatc gacgccgcga atgtaccaga aacgaccgag cagtaaaccg cgatccatcg
     1561 acttgatcat ctcttcaacc gacaccgcgg gctgcgaact ctccatgatg ttattgactg
     1621 gaccaggcgt cggttctttg cctttttgtt tggcccaaaa gcgagagtag acgaggttct
     1681 ccagcacgcc gttgcgcacc agatagattt tttgcgccgg tagtccgccc tgcgccgact
     1741 gcgaagccgg taactccgga tgccacggat cgctgtagat gtttattcgt tcgtcgaaaa
     1801 tcttttcacc aagttttgtc ttgccacccg gcgctgaaaa cgcactccgg ccttcctcgg
     1861 cattgcgcgc gtcgaaactg aaagcaaacg ccacgagcag gtccgcgact gcctgaggtt
     1921 cgagtatcgc cgggtagacg ccggggtcaa gcgcgcgcgg actgcgtgca tcaagcgctt
     1981 tgcgaatggc ttcgcgggcg acgcgcgagg tgtcgagttt tgcaacatcg aagtggttgc
     2041 gcaggaagta gcccgaactg gttccgtcgg gcgtgcgcgc agtcgttgcc aggcttacca
     2101 aactcgagcg ctcgtacacg aagttgccat tcttcgtcgc tgaagcggat gcagatccac
     2161 gcgcctgatg gaaacccgcg cccacgactt ttgccttttc acacgccgaa atcacgtcgt
     2221 tgatcgtttt cgcgcgagcg gcaacagaga tgttcgacgt tgcttccgcg aatggattag
     2281 tcggtttgta agtctgcttt gacaacgttg gcatgtattc gcgatccacg ggtgaaagcc
     2341 gcgcgagttg ttcggccaac gcaacggtgg ctttgagcga ttcatcgtca atctcatttg
     2401 ttgacgcagc gccacgcttt cggtcgatcc aaaccgtcac ggcaacgctg aggttctctc
     2461 tcgcgccgct tgtggtaaag gcgttggctg cgaaacgtag atgaccatag ttttcactgg
     2521 tcacaccgac aacagcatca tcagctttca cgtagctcaa caacttttcg cagattgcgc
     2581 gcgcgtcttt ttcaatgagt agcattacga tctcctcgtt aggttatgag accttacctc
     2641 tacaaccgtt tcattctcac ccggcttcag ccgggtggga gtggcaaagc tttaccagaa
     2701 accgttttaa cggtttcgcc ggaaaaccgt tgaaacggtt cttccattct tcgtaatcgt
     2761 gacacccggc tgaagccggc tgagtatgag aacacgctat gatcgccggg cggtgttgag
     2821 tacgttggtc tggcggaagc gagctaccgg gcaaccgtga ctgacggcgt tcgactggcc
     2881 gggttcaccc ttgccgtcat tgaaactgcc gccgagttca tacgtcgcct gaccacccag
     2941 gccgtcgcac gaattccaga agtcagtagt gcgggattga taagcgacgt cgcgcagcat
     3001 gtccccacgc ttgccgtttt tgatctccca gaacacctgg ccgccaaact ggaagttgta
     3061 ccgctgttga tcgatcgaat aactgccgtc gccgtagatc aaaatgcctt tctccacacc
     3121 ggagatcaga tcgtcgagcg acgtgttgtc cggtccgggc tgaagcgaaa tattcggcat
     3181 gcgcggaaat gagatgctgc cccaactgtc gccgtgattg cagccgtaag acttcttccg
     3241 gccggtcatc gcagcgagat cccgagttgt ttgccaatcg atgaatacgc cgttcttgat
     3301 caggtgccag ctttgggcgc gcacgccctc atcgtcgtaa ccgacagtcg ccagccccgc
     3361 gggttgagtg cgatcggcaa cgaagtggac gatcttggaa ccgaactgaa gtttgccggt
     3421 cttgtcggga gtgatgaagc tcgtgccggc ataattcgct tccatccaga gcgcgcgatc
     3481 gagttcggtc ggatggccca ctgattcgtg gatcgtcagc cacagatgtg acggatgcag
     3541 cacgagatcg tactttccgg cctcgacggg tttcgccttc agcttcatga ccgcttcttc
     3601 gccggcctgt tgcgcttcgc gtttccaatc gtgcttttcc atgtactcgt agcccatgcc
     3661 ctgtggtcca ccgagcgcgc tgcgagattg aaagtcgccg ttgctgcgat taacggcggt
     3721 gacgttgaag ttcggattgg cccggatgat gtactgttcg atacgcgagc cgtcggttga
     3781 tgcataaaac ttctgctcgt tgacgaaccc catcgacgaa cttacaaagc tcacaccgct
     3841 cgtcttcatc gccgattcgt tgaccgacaa caggaactgg atcttgtcgt cgatggaaac
     3901 ttcgaacgga tctttttcga aagcgctctt ccatgaggtg acgatcttct cgacgggaac
     3961 gagtgaaacg cgtttgcgct ggtaggccga gttggcgcgg gcgatctcga cggcttgttg
     4021 cgtgatgcgt cgcacgtctt cgggagtcag gctgcgactc gcagcaaagc cccacgttcc
     4081 tttgtaaagc acgcggacgc cgaagccgaa gttctggctg cgcgacacgt tctgcacctg
     4141 ctgttcgcgc gtggcgatcg actcgttgcg ataacgattt atgcgaatgt cagcataagt
     4201 gacgccgagt ttgcgagcgg tgctcagcgc aacgtcggcg agagcattct tatcgatgcg
     4261 gttcgcggca gcttcttcag cggcgacgac gctgcggaac cattcgggca gtgtagaaag
     4321 cgaaagcgct ccggccgcgg ccccggccgt cttcaaaaaa tcacgacgag aggtagtcat
     4381 cttcaacctc gtttgggaag ggaattgaaa tgcgtgagag atttaaatgg ctggcttaca
     4441 aaaagtcaac gacttcatgc ctggtagttg gtttggtaag ttgtttggcg cggaccagcc
     4501 tataaatctt ctggtttcag acctgtatgc tatgcgattc ggg
//
LOCUS       DASTEY010000339         6121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_435823, whole genome shotgun sequence.
ACCESSION   DASTEY010000339 DASTEY010000000
VERSION     DASTEY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435823"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFO72_05960"
     CDS             complement(<1..329)
                     /locus_tag="VFO72_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478867.1"
                     /translation="MAYTKAVPKSETNIEYLLRDLPDQNGARLFLDRLSNEQPRAYRS
                     VTSQPALLADVLALAAWSPLLATTLEQNPEYLSWLSRERADPRLRSRDELKESLARFA
                     LTNSSLNP"
     gene            complement(383..1807)
                     /gene="glnA"
                     /locus_tag="VFO72_05965"
     CDS             complement(383..1807)
                     /gene="glnA"
                     /locus_tag="VFO72_05965"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET9478868.1"
                     /translation="MNPKTVLKYASDQGAKFVSVRFTDLPGSWQHLTFPIGELGEEQF
                     EDGFGFDASSIRGWAAIHESDMLLVPDATRFWMDPFSEQPTLCLIGSAVDPITKEGYA
                     FDPRSVAQRAESYLKFTGLADTAYFGPEAEFFVFDSVKFQNEPHSACYAIDSDEGHWN
                     SNRDANEFAGSNLGYRIRAKEGYVPVPPTDSLSDLRSEISLTLEKCGVTVECHHHEVA
                     TAGQCEIDFRYSTLLGTADNLQVFKYVVRNTAHEYGKSATFMPKPLFGDNGSGMHCHQ
                     SLWKGEKPLFAGDGYAGLSEMARFYLGGLLKHAAAIVAFAAPTTNSYKRLVPGFEAPV
                     NLAYSARNRSAAIRIPMFSTNPKLKRLEFRPPDPSCNAYLAFSAMLLAGLDGVQNRID
                     PGEPLEKDIYDLSPEELKDVPTLPGSLDDALKALEADHDFLLKGDVFSPQLIDRWISY
                     KRDREITPLRMRPHPLEFSLYYDI"
     gene            2190..4127
                     /gene="speA"
                     /locus_tag="VFO72_05970"
     CDS             2190..4127
                     /gene="speA"
                     /locus_tag="VFO72_05970"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET9478869.1"
                     /translation="MTTAIEQTLEIYGIENWGAGYFDVNRKGNLIVRPVEGDPRSGDL
                     KEIIDDLAKRGITAPVLLRFPQLVAAQVRKLQRAFSRSIREFEYQGSHMCVYPMKVNQ
                     QRAVVEEYLREGSRYDFGLEAGSKAELYAALALEQPGDSLLVLNGFKDEEFIELAFAG
                     AKVGKKVVIVIEKLNELDHVLNVAEREDGPLPMIGMRVKLYSKGSGRWEKSGGEAAKF
                     GLTTTELLEVIRRLEEADRMDMLRLLHFHIGSQLTDIKRIKNAMKEAARVYAKVFQMK
                     APIDFLDVGGGMAVDYDGSRTAFDSSANYTAQEFANDVIYTIKQVCDDENVRHPTIIQ
                     ESGRFLAAYHAILVTNVLDEIETVVEDITPIEIGEDDPQVVIELGDLRESISVKNYRE
                     YYHDALEHREELFTLFNLGLITLEDRAKGEVLFWDVCERADKYAQQAKYVSEEFGELR
                     RLLCAKYLTNFSVFRSVPDHWALDQLFPIVPIHHLNKPPTEYTTLCDITCDSDGIIDK
                     FVDLHDVKQVLELHTLNPDEPYYLAFLLVGAYQEVMGNNHNLFGAPHEAHIHIDEEGF
                     LIKKVIKGTTVGAAAERARYERSLLHDGFRRLINRRVKEGELSEAEGADLAQFYESRY
                     DAYTYLAVNGVKQRSRRRTDY"
     gene            4270..5328
                     /locus_tag="VFO72_05975"
     CDS             4270..5328
                     /locus_tag="VFO72_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET9478870.1"
                     /translation="MVTQRKPKASKYLTTPTRPVQVDRDRSVAGLLEKMEGTGFGGRQ
                     LAEAHRIWLDMLGDNTTIIVSASGALIPAGMRRLLAYVIKNRFVDVLVLSGSMLFHDL
                     HETLGRYHFQAHPSMTDAELESAQISRMWDLLASDEEYREAKEWIGGFTNQLDQTRAY
                     SPREFLHLLGRELAEIASEDGLLTSAYKARVPIFCPAIADSGLSVGIAGARFEKKNNF
                     LFDVIQDILDMMNVMGQSRVSGLISLGGGTTKSFIQQATTSSFLQKEHHHGHKYAIQI
                     STEAAHPGGRSNAISFDEGLIYGKLARGAHTAYVPCDATIALPVLITSLSQTAAKYMK
                     GRKRPNFTFGRELIVEAP"
     gene            5331..>6121
                     /locus_tag="VFO72_05980"
     CDS             5331..>6121
                     /locus_tag="VFO72_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET9478871.1"
                     /translation="MLNRVFPSAFICLLLVFVTLPFHSTAQEDAEVKALYTKTEQMIP
                     MRDGVKLFTAIDTPKDQSQSYPIMLYRTPYSVTPYGLNAYRTALGPSELFQKEKFIFV
                     YQDVRGKFMSEGEFVEVRPYNPKKTGPKDIDESTDAWDTIDWLLKNVPNNNGRAGMWG
                     ISYPGFYTSMGAIDAHPALKAASPQAPIADWFIGDDFHHNGALFLPHAFNFFSTLGKP
                     RPQRTTEFPPRFVHGTPDGYRFFFDLGPLPNANRQYLKGEIAFWN"
BASE COUNT         1448 a         1670 c         1692 g         1311 t
ORIGIN      
        1 ggattaagtg aagagttcgt aagcgcgaag cgggcgagag attccttcaa ctcgtcgcgc
       61 gaccgcagac gcggatcggc gcgctcgcgc gaaagccacg acaagtactc gggattctgt
      121 tcgagtgtag ttgcgaggag cgggctccag gcggcaagcg ccaggacgtc cgcgagcagc
      181 gcgggctgcg acgtgacact gcggtaggca cgcggctgct cgttcgagag ccgatcgaga
      241 aacagccgcg cgccgttttg gtccggaaga tcacggagaa gatattcgat gttggtttct
      301 gacttcggca cggccttagt ataagccacg gacgaacact gattttcact gtcagtgttc
      361 atccgagttc atctgtggcc gcttaaatgt cgtagtacaa cgaaaactcg agcggatgcg
      421 ggcgcatgcg taacggcgtg atctcgcgat cgcgcttgta gctgatccat ctgtcgatca
      481 actgcgggct gaacacgtcg cctttcaaca gaaaatcgtg atcggcttcg agcgctttga
      541 gtgcgtcatc aagcgaaccc ggcaacgtcg gcacatcctt caattcctct ggcgagagat
      601 cgtagatgtc cttttcaagt ggttcacccg gatcgatccg gttctgcacg ccgtccagac
      661 ccgccagcaa catcgccgag aacgcgaggt aagcgttgca cgacggatct ggcggacgaa
      721 actccagccg cttgagcttc ggattcgtcg agaacatggg aatgcgaatc gccgccgacc
      781 gattacgcgc ggagtatgcg agattcaccg gcgcttcaaa accgggcacg agccgtttgt
      841 aactgttcgt cgtgggcgcg gcgaaagcca cgatcgcggc cgcgtgtttc aacaacccgc
      901 cgaggtaaaa gcgggccatc tccgacagtc ctgcatagcc gtcgccggcg aagagcggtt
      961 tttcaccctt ccacaacgac tggtgacagt gcattcccga accgttatcg ccaaacagcg
     1021 gcttcggcat aaacgtcgct gacttgccat actcgtgtgc agtattgcga acaacgtatt
     1081 tgaagacttg caaattatcg gcagtaccca acagcgtgga ataacggaag tcgatttcgc
     1141 attgtccggc ggtggcgact tcatggtgat gacactcgac agtaacccca cacttctcca
     1201 gggtcagcga gatctccgaa cgcagatcgc tcagcgagtc ggtcggcggc accggcacgt
     1261 aaccttcttt cgcgcgaatg cggtagccga ggtttgaacc ggcaaattcg tttgcgtcgc
     1321 ggttgctgtt ccagtgtccc tcgtctgaat cgatggcgta acaagcagag tgcggctcat
     1381 tctgaaattt aacggaatca aagacgaaaa actccgcctc gggaccgaag taagcggtgt
     1441 cggcaaggcc ggtaaacttg agataacttt cggcgcgttg ggcaaccgaa cgggggtcga
     1501 acgcgtaacc ctctttggtt atcggatcga cggcgctgcc gatgagacag agtgtgggtt
     1561 gttctgaaaa cggatccatc cagaagcgag tggcgtcggg gacgagcagc atgtctgact
     1621 cgtgaattgc cgcccagccg cggatggaag aagcatcaaa accaaaacca tcttcgaact
     1681 gctcttcgcc gagttcgccg atggggaacg taaggtgttg ccaggagccg ggtaaatcag
     1741 tgaaacgaac agagacgaac ttggcgccct gatcggaagc atatttcaag acagtcttag
     1801 ggttcatgtg atctcctgaa gttttttggg gagtgttggg ggtgctcttc gccccggcgt
     1861 tgggtttggt gcgaagtatg tgggaaagcg tcagcgtaaa gcgggctgag catcgtaacc
     1921 gaccgcgcgt aaaaaactgg cattataaac aggcactggt ttatgtcaag cctgggaccg
     1981 cagattacac agatagtttt catatatctg tgtaatctgt ggatagtgac ggaaaagtta
     2041 tagaccgccg cagccatttt ctgttacatt gcgcgactaa agcgttccac ccaaacacaa
     2101 ctccgcaact cgattcgcga aatgtacatc agtatttctg agggaattta tatcattcaa
     2161 ggttcagttc gaatgcagga gaccgccaat tgaccacggc aatcgagcaa acgctcgaaa
     2221 tttatggaat cgaaaactgg ggcgctggct actttgatgt aaaccgcaag ggaaacctta
     2281 tcgtccgacc cgtcgaaggc gatcctcgct caggggatct caaagagatt attgatgacc
     2341 tggccaaacg cggaatcacc gcgcccgtat tgctgcgttt tccacaactc gttgcagccc
     2401 aggtgagaaa acttcaacgc gcgttcagcc gctctatccg cgagtttgag taccagggtt
     2461 cgcacatgtg cgtttacccg atgaaagtaa accagcaacg cgcggtcgtc gaggagtacc
     2521 tgcgcgaagg ctcgcgctac gattttggac tcgaagccgg ttccaaagcc gaactttacg
     2581 ccgctctagc acttgaacag ccgggcgaca gcctgctggt gctgaacggt ttcaaggacg
     2641 aggagttcat cgagctcgct ttcgccggtg caaaagtcgg aaagaaagtg gtcatcgtaa
     2701 tcgagaagct gaacgagctc gatcacgtgc tcaacgttgc cgaacgtgaa gacggaccac
     2761 tgcccatgat cggcatgcgc gtcaagcttt attcgaaagg ctcgggacgc tgggagaagt
     2821 cgggcggcga agcagccaag tttggcttga cgaccaccga gttgctggaa gtgatccgcc
     2881 gtctggagga agcagaccgg atggacatgc tgagactgct ccacttccac atcggctcgc
     2941 aactcaccga catcaagcgc atcaagaatg cgatgaagga agccgcacgc gtctacgcaa
     3001 aggtcttcca aatgaaggcg ccgatcgatt ttctcgacgt cggcggcggc atggcggtcg
     3061 attacgacgg ttcaagaaca gcctttgatt cgtcggcaaa ttacaccgcg caggagtttg
     3121 ccaacgacgt catctacacg atcaaacagg tctgcgacga cgagaacgtg cggcatccga
     3181 cgatcatcca ggagtcggga cgttttctgg ccgcgtacca cgcgattctc gtcaccaacg
     3241 tgctggacga gattgagacc gtcgtagaag atattacgcc gatcgagatt ggtgaagacg
     3301 atccacaggt agtgatcgaa ttgggcgatc tgcgcgagtc gatcagcgtc aagaactatc
     3361 gcgagtacta tcacgacgcc ctcgaacatc gtgaagagtt gtttacgtta ttcaatcttg
     3421 gcctgatcac gctggaagat cgcgccaagg gcgaagtgct tttctgggac gtttgcgaac
     3481 gcgccgacaa gtatgcgcag caggcgaagt acgtttccga ggaatttggc gaactgcggc
     3541 gcctgctttg cgcgaagtac ctgacgaatt tctcggtgtt tcgttcagtg ccggaccact
     3601 gggcgctgga ccaactgttt ccgattgttc cgattcacca tctgaataag ccaccgacgg
     3661 aatacaccac gctctgcgac atcacctgcg actccgacgg aatcatagac aagtttgtcg
     3721 acctgcacga cgtgaagcag gtgctcgagt tacataccct taacccggac gagccgtatt
     3781 acctcgcttt cctgctggtc ggcgcatacc aggaagtgat gggtaacaat cacaatcttt
     3841 tcggcgcgcc ccacgaggcg cacatccata ttgacgagga gggctttctt atcaagaagg
     3901 ttattaaagg aaccaccgtc ggcgccgccg cggaacgcgc gcgttacgag cgcagtttgc
     3961 tgcacgacgg cttccggcgt ttgatcaatc ggcgcgttaa agaaggcgag ttgagcgaag
     4021 cggaaggcgc cgatctggcc cagttctatg aatctcgtta cgacgcttac acgtatctcg
     4081 cagtcaacgg cgtgaagcag cgttcgcgca gacgaacgga ctattgatat tgccaattgc
     4141 caattgccaa ttgccgattt gaagaagtgc tgcgcatcgt cagttgtttg aaattggcaa
     4201 tcggcaatcg gcaatcggca ataacaacaa tcggcaattg gcaatcacaa cacgaggttg
     4261 acaacctgca tggtcactca acgaaaacca aaagcatcga agtacttgac cactcccacg
     4321 cgcccagtcc aggtggaccg cgatcgttcg gtggccggac tcctcgaaaa gatggaaggc
     4381 acgggtttcg gtggccgcca actcgcagag gcgcatcgca tctggctcga catgctcggc
     4441 gacaacacga ccatcatcgt ctccgcgtcg ggcgcgctca ttcccgcagg catgcgacgg
     4501 ctgctggctt atgtaatcaa gaatcgattt gttgacgtgc tggtgctctc gggttcgatg
     4561 ttgtttcacg atctgcatga aacactcggc cgttatcatt ttcaggcgca cccgagtatg
     4621 acggacgcgg agttggagtc ggcgcagatc agccgcatgt gggacttgct ggcaagtgac
     4681 gaagagtatc gcgaagcaaa ggagtggatc ggcggcttta caaatcaact cgaccagaca
     4741 cgcgcctact cgccgcggga gtttctgcat ctgcttggcc gtgaactcgc agagatcgca
     4801 tccgaagacg gtttactgac ttccgcctac aaagcgcgcg taccgatctt ctgtccggcg
     4861 atcgctgata gtggactctc cgtcggcatc gcgggtgcac gctttgaaaa gaagaacaac
     4921 ttcttgttcg acgtcatcca ggacatactg gacatgatga atgtgatggg ccagtcgcgt
     4981 gtgtcgggcc tcatcagttt gggcggcggc acgacgaaga gtttcattca acaagcgact
     5041 acctcttcgt ttttgcagaa agaacatcat catggtcaca agtacgcgat tcagatctcc
     5101 actgaagccg ctcatccggg cggcagatcg aacgccatat cgtttgatga aggattgatc
     5161 tacggaaaac tggctcgcgg agcgcatacc gcttacgttc cctgtgatgc aaccattgct
     5221 ctccccgtac tcatcacttc actgtcacaa acagcggcaa agtacatgaa gggacgcaaa
     5281 cgtcctaact ttactttcgg ccgcgagtta atcgtcgaag caccctaatt atgcttaatc
     5341 gcgtttttcc gtccgcgttt atctgtttac tcctcgtctt cgttactcta ccgtttcatt
     5401 ccacagcgca agaagacgcc gaagtcaaag ctctctatac caagaccgag caaatgatcc
     5461 cgatgcgcga cggcgtgaag cttttcaccg ccatcgacac gcccaaggac cagtcgcagt
     5521 cgtatcccat catgctctat cgcacgccct acagcgtgac gccctacggg ctcaacgcat
     5581 acagaactgc acttggacca tccgaactct ttcaaaaaga gaagttcatc ttcgtctatc
     5641 aggacgtccg tgggaagttc atgtcggaag gcgaattcgt cgaggtgcgg ccgtacaacc
     5701 caaagaaaac cggaccaaaa gatattgatg aaagcaccga cgcatgggac acgatcgact
     5761 ggctcctcaa aaacgtaccg aacaacaacg ggcgcgccgg catgtgggga atttcgtatc
     5821 ccggtttcta tacctcgatg ggcgcgattg acgctcaccc cgctttgaaa gcggcgtcgc
     5881 cgcaggcgcc gattgccgac tggttcatcg gtgacgattt tcatcacaac ggcgcgctgt
     5941 ttctgccgca cgcgttcaac ttcttctcga ccttgggcaa acctcgtccg caacgaacta
     6001 cagagtttcc gccgcgcttc gtgcacggaa ctcccgacgg ctaccgtttc ttttttgatc
     6061 ttggcccact tccgaatgcg aaccgccaat acttgaaagg tgagatcgcc ttctggaacg
     6121 a
//
LOCUS       DASTEY010000340         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1078809, whole genome shotgun sequence.
ACCESSION   DASTEY010000340 DASTEY010000000
VERSION     DASTEY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078809"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1122)
                     /locus_tag="VFO72_05985"
     CDS             complement(157..1122)
                     /locus_tag="VFO72_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9478872.1"
                     /translation="MKTPRLFLALFLSLVFAAPVIAQIREENIRAELGFLASDAMQGR
                     ASGSNYERIAAEYIGSQFRQFGLEPGGDTDSAGNKSFVQRVPLERAPSGATWNAIGVL
                     RGSDRRAAGEVIMISAHLDHIGVNEALTGDDKIFNGADDDASGSVAVMELARVLASGK
                     RPRRTIYFVCFGSEERGGHGSRYFIANPPVPLTQIVADLNLEMLGRPDPKVPAGTLWL
                     TGFERSTLGPELARQGAALVADPRPEQNFFRRSDNYILALRGVIAHTVSSFGMHTDYH
                     RPSDDVSKIDFPFMTKSLNSLVKPVRWLANSKFRPTWLPGQAPTR"
     gene            complement(1142..>2026)
                     /locus_tag="VFO72_05990"
     CDS             complement(1142..>2026)
                     /locus_tag="VFO72_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET9478873.1"
                     /translation="ATLPASLAGMHQPTGRAMEVRLAGRPSLDSSDVNRFSAVVFGEG
                     DFHVRTEDRPLPPALKSGDTIVLGPLHARVEKLLGHPRLVELAFVGTADEVWAGLASH
                     GRPIQYSHMQTSLELWDVWTRIAGPPVAFEPPSAGFVLDWHVLSAIREHQAGFATITH
                     AAGLSSTGDEDLDRLLPFDEPYRIPVTTAKAVEQTRQRGGRVIAVGTSVVRALEGAAT
                     EDSHLRSGEGLAKGRIGPATRLKVVDAILSGTHEPGTSHYELLRAFLEDSTLERATAE
                     LDARGYRTHEFGDSVLIF"
BASE COUNT          444 a          599 c          559 g          424 t
ORIGIN      
        1 ttattagcca caaaaaggca caaaaagcac aaaatgaatt ttcaagaaat cttttgtgct
       61 ttttgtgcct tttttgtggc tactcctctt ttggcttcag ttcgagtacg cctgacttca
      121 cgcccttctc cgaactgaag aacgttggtt tcatagttag cgtgtcggtg cttgtcccgg
      181 cagccacgtg ggacgaaatt tggaattagc taaccagcgc accggtttca ccagcgaatt
      241 cagcgatttc gtcatgaaag ggaaatcgat cttgctgacg tcgtcactgg gacgatgata
      301 atcggtgtgc atgccaaagc tggaaacagt gtgagctatc acgccgcgca acgccagtat
      361 gtaattgtcg gagcgtcgaa agaagttttg ttcgggacgc ggatcggcga cgagcgctgc
      421 gccttgtcgc gccagctcgg ggccgagtgt cgatcgctcg aagccggtta gccacaacgt
      481 gccggcggga actttcggat cgggtcgacc gagcatttcc aggtttaaat cggcgacgat
      541 ctgcgtcagc ggcactggcg gatttgcaat gaagtatcgc gaaccatgac cgccgcgctc
      601 ttcgcttccg aaacagacga agtagatcgt gcgtcgcggg cgtttgcctg atgcgagcac
      661 gcgcgcgagc tccatgacgg cgacactgcc tgaggcatcg tcgtcggcgc cgttgaagat
      721 cttgtcgtcg ccggtaagtg cttcgttaac gcctatgtgg tccaggtgag cgctaatcat
      781 gatcacctca ccggcggcgc gccgatcgga acctcttaac acaccgatcg cattccaggt
      841 cgcacctgat ggcgcacgtt cgagcggcac gcgctgcaca aaactcttgt tgccggcgct
      901 gtcagtatca ccaccgggct cgagtccgaa ctggcgaaac tgagagccga tgtattcagc
      961 cgcgatgcgc tcgtagttgc tgccgctggc acggccctgc attgcgtcac tcgcgagaaa
     1021 gccgagctcg gcgcgaatgt tttcttcgcg tatctgtgcg atgacggggg cagcgaaaac
     1081 aagagagaga aagagggcga gaaacaaccg gggcgttttc atagacgcgt ataatacatg
     1141 cttaaaagat taggaccgaa tcaccgaatt cgtgagttcg atatccacgc gcgtctaatt
     1201 ctgctgttgc gcgttcgagc gtcgagtcct cgagaaacgc gcgtagcagt tcgtaatgac
     1261 ttgttcccgg ttcgtgcgtg ccggaaagaa tcgcgtccac aactttcaag cgcgtcgcag
     1321 gaccgatgcg tccttttgcg agaccttcac cactgcgcag gtgactatct tcagtcgccg
     1381 caccttcaag tgcgcgcacc accgacgtac caacggcgat gacacgcccg ccacgctgcc
     1441 gcgtctgctc cactgctttc gccgtcgtca ccggaatacg atacggctcg tcaaacggca
     1501 gcagtcgatc cagatcttcg tcaccggttg aagagagccc cgcggcatgc gtgatcgttg
     1561 caaagccagc ctgatgttcg cgaatagctg acagcacgtg ccaatcgaga acaaagcccg
     1621 ccgaaggtgg ttcgaacgcc accggtggtc cggcaatcct ggtccaaacg tcccacagct
     1681 ccagggacgt ctgcatgtgt gaatactgaa tcggccggcc atgactcgcc agtcccgccc
     1741 agacttcatc cgccgtcccc acaaaagcaa gttccaccaa tcgcggatgg cccaacagtt
     1801 tctcaaccct ggcatgcagt ggaccgagca cgatcgtgtc tccactcttc aacgccggcg
     1861 gcaacggcct gtcttcagtg cgtacgtgaa aatctccctc accaaaaacg accgcactga
     1921 aacgattaac atccgacgaa tcgagagaag gacgtccggc aagacgcacc tccatcgccc
     1981 ttcctgtcgg ctgatgcatg cccgccaggc ttgccggcaa cgtggc
//
LOCUS       DASTEY010000341         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_419432, whole genome shotgun sequence.
ACCESSION   DASTEY010000341 DASTEY010000000
VERSION     DASTEY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419432"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1438)
                     /locus_tag="VFO72_05995"
     CDS             complement(77..1438)
                     /locus_tag="VFO72_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET9478874.1"
                     /translation="MDYTQAEILQTVAMTEMEHLDVRTVTLGLSLLDCASDSLSTMAQ
                     RAGEKIRTVASRLVPVVDEIGEELGIRVANKRIAITPVSLLTEGAKGNPIELARAIDD
                     AAGTVGVDFIGGYSALVQKGTTPSERVFLDSIPEALSSTTRLCSSVNAATTRAGINMD
                     VVAQIGRVIKDAAERTRDQGGLACAKFVCFANAVEDNPFMAGAFHGVSEADAVINVGV
                     SGPGVVHYAVRHADRTLPLQDLAETIKKLSFKLSRAGELVGREAARRLGIPFGIIDLS
                     LAPTPVHGDSVANIIEAMGFERAGTHGTTAALALLTDAVKKGGAMASGSVGGMSGAFI
                     PVSEDAGMIEAAQVGALSLEKLEAWTSVCSVGIDMVAVPGSTSAETIAAIIADEMAIG
                     VMNNKTTAVRIIPVPGRDVGEVVEFGGLLGSAPIMAVSAFSCAEFIRRGGRIPAPIHS
                     LRN"
     gene            complement(1443..1751)
                     /locus_tag="VFO72_06000"
     CDS             complement(1443..1751)
                     /locus_tag="VFO72_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478875.1"
                     /translation="MSEPEIARRCSACGVSLRQRAMFCPQCGEKISDNQVREHPTPAP
                     SALTGVAENVRDRVEKIRHVSSVVIDQAAYDPSLRFLLVAAVLFVLFLVLLILSKLIG
                     "
     gene            complement(1769..2200)
                     /locus_tag="VFO72_06005"
     CDS             complement(1769..2200)
                     /locus_tag="VFO72_06005"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9478876.1"
                     /translation="MANEDLIYRITRAVYDRLGPGADEQMVEQLVTDIYREVNQSSPV
                     ATNSPSTGSSGRMIISVFGLDHPGIVAGVSQILAEAGCSIIDINQTVVQGKFAMVIIA
                     DTARARESTAELKERLRREGEHLGVRIYAQREDLFNAMHRV"
     gene            complement(2291..2620)
                     /locus_tag="VFO72_06010"
     CDS             complement(2291..2620)
                     /locus_tag="VFO72_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9478877.1"
                     /translation="MFLAKVVGTVWSTKKTPDLEGVRFLVVHPYDLDKEPTRNIVVVA
                     DRLGAGTGEMVMCAFGKAARTAIGNQDMSIEAAVVGIVDRVDINETFSEEMRDAARRL
                     AHENGKP"
     gene            complement(2685..>3190)
                     /locus_tag="VFO72_06015"
     CDS             complement(2685..>3190)
                     /locus_tag="VFO72_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478878.1"
                     /translation="GNVTSDNVSPLHLMDIKRVAFETRPVKSARPAAGVQPVVSAPQT
                     QPAPAPVIAGGKIDRQEIAAIVDRFLAGKRIEAPPVAAPLPKPEIEAEAGSMYAPAAP
                     KIVEPVTKPAPPPSSNGNRAAVEFVSEDDVKRAIQRGDKIYINSKTIITPAARDIGEP
                     AEVFAKV"
BASE COUNT          663 a          948 c          885 g          694 t
ORIGIN      
        1 ccgcggcatt caaccattcc gagtgcttca ggcattgcga cctcctggcg aagggtaaca
       61 cggagtaata cgctttttaa ttgcgaagtg aatgaatggg tgcaggaatg cgaccgccgc
      121 gacgaataaa ctcagcacac gagaacgcgc tcaccgccat gattggagca ctgcccaaca
      181 gcccgccaaa ctcgaccacc tcgccgacgt ctctgcccgg cacgggaatg atgcgcacag
      241 ccgtcgtttt gttattcatc actccaatcg ccatttcgtc ggcaattatc gccgcgatcg
      301 tctccgccga agtcgatccg ggaacagcga ccatgtcgat accgacggaa cagacgctgg
      361 tccatgcttc gagtttctcc agggacagtg cgcctacctg ggcggcttcg atcatgcccg
      421 catcttcgga gacgggaatg aacgcgccgc tcatgccacc gaccgaaccg ctcgccatcg
      481 cgccgccttt cttgacagcg tcggttaaca gagcgagggc ggccgtcgtc ccgtgtgtgc
      541 cggcccgttc gaaacccatc gcctcgatga tgtttgccac tgagtcgccg tggaccggag
      601 tcggcgccaa agagagatcg atgatgccaa atggaattcc caaacggcgc gctgcttcgc
      661 gtccgaccaa ttcaccggcg cgggagagtt taaaggagag tttcttgatc gtttcggcga
      721 gatcctgcaa aggtagagta cggtccgcgt ggcgcacggc gtaatggacc acgccgggac
      781 cagacacgcc gacgttgatt acagcgtccg cttcgctgac cccgtgaaac gctccggcca
      841 tgaacgggtt atcttcgacg gcattcgcaa aacagacaaa ctttgcgcag gcgagtccgc
      901 cttgatcgcg cgtccgttcg gcagcgtctt tgataacgcg tccgatctgc gccacgacgt
      961 ccatgttgat gccggccctc gtcgtcgctg cattgaccga cgaacacaaa cgggtcgtgc
     1021 tcgacagcgc ttcgggaatt gagtcgagaa acaccctttc cgacggtgtc gtacctttct
     1081 ggaccagcgc tgaataacca ccgatgaagt cgacgccgac agttccggca gcatcgtcga
     1141 tcgcgcgcgc gagttcgata ggatttcctt tcgcgccttc ggtaagaagt gaaacaggcg
     1201 tgattgcgat gcgcttgttg gcgacgcgga taccgagctc ttcgccgatc tcgtcaacaa
     1261 ccgggaccag acgcgacgcg acggtacgga tcttctcccc cgcacgttgc gccatagtag
     1321 agagtgaatc gctcgcgcag tcgagcaggc tgaggccgag tgtgacggtg cgcacgtcga
     1381 gatgctccat ctcggtcatg gcgacggttt gcaggatttc ggcctgagtg tagtccatgc
     1441 gtttagccga tcagtttgct gagaatcagc aagacgagga agagcacaaa cagcactgcc
     1501 gcgaccagca ggaaacgtaa actcggatcg tacgcagctt gatctataac gacgctggag
     1561 acgtgccgaa tcttctcgac gcgatctcgc acattttcgg cgacgcctgt taatgcagaa
     1621 ggagcgggag tgggatgttc acgcacctgg ttgtcactga ttttttcgcc gcactgcgga
     1681 caaaacatcg cgcgctggcg aagcgaaact ccacaggcgg aacaacggcg ggcaatttca
     1741 ggctctgaca tagacggttc gggaattttc acactctatg catcgcgttg aaaaggtctt
     1801 cgcgctgcgc gtaaatgcgc acgcccaaat gttctccttc gcgccgcaat cgctccttca
     1861 gttcagcggt gctctcacgc gctcgtgcag tgtcggcaat aatgaccatc gcaaactttc
     1921 cctggacgac ggtttggttg atgtcgatga tactgcaacc agcctcggcg aggatctgcg
     1981 acacacctgc gacgattccc ggatggtcga gaccaaacac cgaaatgatc atgcgaccgg
     2041 acgatcccgt agacggagag ttggttgcga ccggacttga ctgattcacc tcacgataga
     2101 tgtccgtaac caactgctcc accatctgtt cgtccgcacc tggtcctaag cggtcgtaaa
     2161 ccgcgcgagt gatgcgatag atcaaatctt catttgccat caggggattt tcgattgacg
     2221 attttcgatt gccgattgct aagactgccg gatcaaaatc ggcaatcgcc aatcggcaat
     2281 cggcaatgtc tcagggcttg ccgttttcgt gcgcgagccg gcgcgccgcg tcgcgcatct
     2341 cttctgaaaa cgtttcgttg atgtccacgc gatcgacgat accgacgaca gcggcttcga
     2401 tcgacatgtc ctgatttccg atcgccgtcc ttgctgcttt gccgaaggcg cacatcacca
     2461 tctcgcccgt tccggctccg agtcgatcgg caactacgac gatatttcgg gttggttctt
     2521 tgtcgagatc gtaagggtga acgacgagga accggacacc ttcgagatcg ggtgtcttct
     2581 tggtggacca gactgtgcct actactttcg ctaagaacat tgtgctttga gctttgtgct
     2641 ttgtgctttg tccttaccaa gaacaaagca ctaagtacca agtactaaac cttcgcaaac
     2701 acctccgccg gttcaccgat gtcgcgcgcg gccggtgtaa ttatcgtctt tgagttgatg
     2761 tagatcttat ccccacgctg gatcgcccgc ttcacgtcgt cttcgctgac aaactcaacc
     2821 gctgctctgt ttccattgga cgaaggcgga ggcgcgggtt tagtcaccgg ctcgacaatc
     2881 ttcggtgcgg caggggcgta catgcttcct gcttcagcct caatctctgg tttcggtaac
     2941 ggcgccgcta ctggcggcgc ttcgattcgt ttaccagcca aaaaccgatc cacgatggcc
     3001 gcgatctcct ggcgatcgat cttgccgccc gcgatcacag gcgccggggc aggctgcgtt
     3061 tgtggtgcac ttacaacggg ctgcacacca gccgccggac gtgcgctctt caccggccgt
     3121 gtttcaaacg cgactcgctt gatgtccatc aaatgcagcg gcgagacatt gtccgaagtg
     3181 acgttgccgc
//
LOCUS       DASTEY010000342         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1227081, whole genome shotgun sequence.
ACCESSION   DASTEY010000342 DASTEY010000000
VERSION     DASTEY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227081"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFO72_06020"
     CDS             <1..1087
                     /locus_tag="VFO72_06020"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HET9478879.1"
                     /translation="PITPPPIIATSTLLCLSLTVQSLILKPNMHPITLIPGDGIGPEV
                     ASNVVRIIEASGVDIQWETHYAGAQALEKFGETLPQELLDSIIRNKVALKGPITTPIG
                     KGFTSVNVGLRKTLDLYANLRPVKALPNVPCRYPELDLIVVRENTESLYAGLEHVVVP
                     GVVESLKIITEKASTRIARFAFEHAQREGRKKVTAVHKANIMKLSDGLFLDCFRRMAQ
                     EFPQIEADDKIIDNACMQLVMRPEQFDVMLLENLYGDIVSDLCAGLVGGLGLVPGANF
                     GEKGAVFEAVHGSAPDIAGQGIANPTALLQSGILMLRYIGERDAAARIENAMMAVFAE
                     GKVRTRDIGGTAKTAEFADAIIQKLHS"
     gene            1207..1419
                     /locus_tag="VFO72_06025"
     CDS             1207..1419
                     /locus_tag="VFO72_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET9478880.1"
                     /translation="MGLPNRDEMEGKWDQAKGKTKEVVGRALDDEELEAEGSAERLGG
                     KAEEGFGKARRNVGEAIEDVGDAIKR"
     gene            complement(1480..1995)
                     /locus_tag="VFO72_06030"
     CDS             complement(1480..1995)
                     /locus_tag="VFO72_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478881.1"
                     /translation="MADKEKTKLVTGLFKSKVAAESAVDALLKRGFTRDDISVLMSDE
                     TRSKHFALQSRTHAADGLGIGAAAGGTVGAVLAAITAVGTTLFLPGLNLVIAGPIAAA
                     LAGLGAGGAAGGLIGALIGAGIPEYRAKVYEAGIRGGGILIGVEARDEEEVDRLEELL
                     SELGADNVRTE"
     gene            2117..3214
                     /locus_tag="VFO72_06035"
     CDS             2117..3214
                     /locus_tag="VFO72_06035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9478882.1"
                     /translation="MRILQISSAASFGGGERYAADLANTLQERGHDIYAAVRRQSPLI
                     AQLRLSAAKIKTLPLRNALDVQSAHELARFVRKHEIEIVHAHMARDYSLAAYAAHRNR
                     NTKFIVTRHVLFPLSRLHRQTLARATRVIAVSNAVARELKAAKLMNKDRITVVHNGID
                     IARFARVLAGFDRAEFLRSKGLPEDCLLAGSIGELRTLKRHDDFIRAAAMIAPQFEKV
                     HFVLAGVDTSSTHEIKQQLERLVSELRLRDRFHFLGWLDDAEKLLAAMDVFVSASETE
                     SFGLAIAEAMAAGSAVVATATEGAREVVEDQKTGLLVPIGNVEQIAENVARLLADAER
                     RKEISARAKEAVAARFSLQRMVDEIEAIYGS"
BASE COUNT          736 a          893 c          920 g          668 t
ORIGIN      
        1 tccaatcacc ccaccgccga taatcgcaac gtcgacactt ctgtgtttgt cactcacggt
       61 ccaaagtctt atcctaaagc cgaacatgca tcctatcaca ctgattcccg gcgacggcat
      121 cggccccgaa gtcgcttcca acgtagtccg aatcattgaa gcttccggcg tcgatattca
      181 atgggaaacc cattacgccg gcgctcaggc gctcgaaaaa tttggcgaga cgctgccgca
      241 ggaactgctc gattcgatca ttcgcaacaa ggtcgctttg aaggggccaa tcaccacgcc
      301 gattggcaaa ggttttactt cagtcaacgt cggattgcgc aagacgctgg acttgtatgc
      361 gaacttgcgg ccggtaaagg cgctgccgaa tgtgccgtgt cgttatccgg aactcgatct
      421 catcgtggtg cgagagaaca cggagagttt gtacgcggga ttggagcatg tcgttgtgcc
      481 gggtgtggtt gagagtttga agatcatcac ggagaaggcg tcaacgcgta ttgcgcgctt
      541 tgctttcgag catgcgcagc gcgaagggcg gaagaaagtg acggcggtcc acaaggcaaa
      601 cattatgaaa ctgtcggatg gtctgtttct cgactgcttt cgccgcatgg cgcaggagtt
      661 tccgcagatt gaagcggacg acaagatcat cgataacgcg tgcatgcaac tcgtgatgcg
      721 gccggagcag tttgacgtca tgctgctcga gaatctttac ggcgacatcg tttcggatct
      781 ttgtgcgggt ttggttggcg gtttggggct ggttcccggc gctaactttg gtgagaaggg
      841 cgcggtgttc gaagccgtgc acggttccgc gcctgacatc gcagggcagg gcatcgccaa
      901 tcccacggcg ctgttgcaat caggcatttt gatgctgcgt tacatcggcg agcgcgacgc
      961 cgccgcgcgc atcgaaaacg cgatgatggc ggtctttgcc gaaggcaaag tacgcacccg
     1021 cgacatcggc ggcaccgcca agaccgccga gtttgcggac gcgatcattc agaagctgca
     1081 ctcgtaactc gcttagcaaa aagttgaact cgtttcaagg atgtttggtt gatcaagcgc
     1141 gctgtggaac gctggttgct cctcaggtgt gtgtgaagaa atcatcaagg tatcaggagg
     1201 gttaagatgg gattaccaaa cagggatgag atggaaggca aatgggacca ggctaaaggc
     1261 aaaacgaaag aagtagtcgg cagagcgttg gacgacgagg agctcgaggc agaaggcagt
     1321 gctgaacgcc tcggcggaaa agccgaagaa ggtttcggta aagcgcgacg caacgtcggc
     1381 gaagctatcg aagatgttgg cgacgcaatc aagcgttaac taattttgac cacggattac
     1441 acggattcgc atccgtgtaa tccgtgtaca actgtggccc tactcagtgc gcacgttgtc
     1501 cgcgcccaac tcactaagca gctcttccag ccgatccact tcttcctcgt cgcgcgcttc
     1561 aacaccgatc agtattccac cgccacgaat gccggcttca taaactttcg cgcgatattc
     1621 aggaataccc gcgccgatca aagcgccaat caaaccgccc gccgcgccgc ctgcgccgag
     1681 tccggccaac gcggccgcaa ttggaccagc aatgacaaga ttcaacccgg gcagaaagag
     1741 tgtcgtgccg accgcggtaa tcgcagcgag caccgcgccc accgtgccgc ccgccgcagc
     1801 gccgataccg agtccatccg ctgcatgcgt gcgtgattgc aacgcaaaat gtttcgagcg
     1861 ggtctcgtcg gacattagca cgctgatgtc gtcacgtgta aaaccgcgtt tcaaaagagc
     1921 gtccaccgca gattcggcgg ccaccttact cttaaaaagt ccggttacca atttcgtttt
     1981 ttccttatca gccatagaat cctccccaac gcaaagttcg cttcagggat acatgggcaa
     2041 catttgtgcc gattgtgtca ccgctgataa acggaagact acgcgcgaag gctgtttggc
     2101 tttcggacat cactagttga gaatccttca aattagttca gcggcatcct tcggcggcgg
     2161 cgaacgttac gctgccgatc tggctaacac tcttcaggaa cgtgggcacg acatctacgc
     2221 cgctgttcgc cggcaatcac cgttgatcgc gcaactccga ttgtctgccg cgaagatcaa
     2281 gacgctgccg ctgcgcaacg cgctcgacgt gcaaagcgcc cacgagctcg ctcgtttcgt
     2341 tcgcaagcac gagatcgaaa ttgttcacgc gcacatggcg cgcgactact cgctcgcagc
     2401 ttacgccgca catcgcaatc gcaacacgaa gttcattgtc actcggcacg tgctctttcc
     2461 actcagtcga ctccaccggc agacgctcgc gcgcgccacg cgtgtgattg ctgtttcaaa
     2521 cgccgtggcg agagaactta aagctgcgaa gctcatgaat aaagatcgga taacggtcgt
     2581 gcacaacggc atcgacatcg ctcgcttcgc gcgagtgctc gctggtttcg accgcgcgga
     2641 gttcttacga agcaaaggac taccggaaga ttgtttgctc gccggcagca ttggcgagct
     2701 gcgaacgctg aaacggcacg acgactttat tcgcgccgcg gcaatgattg ctccacagtt
     2761 cgagaaggtt cactttgtgc ttgccggtgt cgacacctca agcacgcacg aaattaaaca
     2821 acaactcgag cggcttgtat ccgagctgcg tctcagggat cggtttcatt tcctcggctg
     2881 gcttgacgac gcggaaaagc tgctggctgc aatggacgtt tttgtctcag cgtccgaaac
     2941 ggaatcgttt ggcctggcca tcgccgaggc catggccgcg ggaagcgcgg tagttgccac
     3001 ggcgactgaa ggagcgcgtg aagtagtcga agatcaaaag acgggcttgc tggtcccaat
     3061 cggcaacgtc gaacagatcg ccgaaaacgt agcgagacta ctcgctgatg cagagcgacg
     3121 gaaagagatt tccgcgcggg cgaaagaggc cgttgccgcg cggttcagtt tgcagcgaat
     3181 ggtcgatgag attgaagcaa tctatgggag ttaacgt
//
LOCUS       DASTEY010000343         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1227137, whole genome shotgun sequence.
ACCESSION   DASTEY010000343 DASTEY010000000
VERSION     DASTEY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227137"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFO72_06040"
     CDS             <1..641
                     /locus_tag="VFO72_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9478883.1"
                     /translation="RVAIQALAGVLGGTQSLHTDAYDEALALPTDRAALIALRTQQII
                     AEETGVANTVDPLGGSYYLEALTEKMEQETLDYFRKIDAMGGMVAAIERGFPQREIQD
                     SAYQYQKAVERGEQVIVGVNKYAMSDEPAEVPILVIDESVRTHQVERLEQTRVRRDAG
                     AVANALEKLKLAAATNENTMPATIDAVRAYATLGEICSALRDVYGVYEEPAF"
     gene            644..1048
                     /locus_tag="VFO72_06045"
     CDS             644..1048
                     /locus_tag="VFO72_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET9478884.1"
                     /translation="MSTRKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEMIAAAALQEDVDAVGVSILSGAHNTLCPRIVQLLREKGMDNCLVLVGGIVPQEDVP
                     KLKQQGVAEVFLPGTSTGDIVKFLSDNVKPRE"
     gene            complement(1167..1940)
                     /locus_tag="VFO72_06050"
     CDS             complement(1167..1940)
                     /locus_tag="VFO72_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9478885.1"
                     /translation="MADEQPVLYSVEGALARIQLNRPEKRNALNAALVAGLKEKLHAA
                     GKDERVRVVMISGAGKDFCSGADLSALQTIAQASVSENAEDARSLLDLFVLIRQIPVP
                     VVAAVRGRALAGGCGLASACDIVLASASARFGYPEVKIGFVPAMVMAILRRNVSEKIA
                     FELLTRGAEISAEQALESGLVNHIFADETFDDDVTAYLNEFSKLSRSAISLTKTLLYQ
                     MDGLTFSAALETGADVNVIARLTKDCQEQVAGFLNRTKK"
     gene            complement(1941..2285)
                     /locus_tag="VFO72_06055"
     CDS             complement(1941..2285)
                     /locus_tag="VFO72_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478886.1"
                     /translation="MKVQLTNLAHARSGDKGDTANVGLIALREEVYPILVREVTAERV
                     KKHFTGICRGEVERFELPNLGALNFLLHESLGGGGTLSLMTDAQGKTFSTALLRMEIE
                     VSDEEARKLELN"
     gene            complement(2282..>2591)
                     /locus_tag="VFO72_06060"
     CDS             complement(2282..>2591)
                     /locus_tag="VFO72_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478887.1"
                     /translation="DRLGLQFDYVLTEFVGVNATHGPLAGEPRADLPEVQLRFGVRGQ
                     DRGAIERFTKEIAPLILTGPPAVTGFAGGRPKVEEIVAYWPALIPKKEIMTEIEVLEA
                     "
BASE COUNT          575 a          797 c          691 g          528 t
ORIGIN      
        1 tgcgcgtagc gatccaggcg ctggccggcg tactcggcgg cacccaaagc ctgcacaccg
       61 acgcctacga cgaagcgttg gcactgccaa ctgatcgcgc ggccctgatc gccctccgca
      121 ctcagcagat catcgccgaa gaaacgggcg tcgcgaacac ggtcgatccg ctgggtggct
      181 cctactatct ggaagcacta acagaaaaga tggaacagga gacgctcgac tacttcagaa
      241 aaatcgacgc gatgggcggc atggtcgcag cgatcgaaag aggatttccg cagcgcgaga
      301 ttcaggactc cgcttatcaa tatcagaaag ctgtcgaacg cggcgaacag gtgatcgtcg
      361 gggtcaacaa gtatgcaatg agtgacgaac ctgccgaagt tcccatcctt gtgatcgacg
      421 aatctgttcg cactcaccag gtcgagcggc tcgaacaaac acgggtgcgg cgcgacgcag
      481 gcgcagttgc aaacgcgctc gaaaaactca agctggccgc cgccacaaac gagaacacga
      541 tgccggcaac gatcgacgcc gtccgcgcgt acgcaactct tggtgaaatc tgttccgcgc
      601 tgcgtgacgt ttacggcgtc tacgaagagc ccgccttctg aatatgtcca ctcgaaagat
      661 tcgtgtgctg gtagcaaagc ccggccttga tggtcacgac cgcggcgcca aagtgatcgc
      721 gcgcgcactg cgcgatgcgg gcatggaagt gatctacacc ggcttacgcc agacgcccga
      781 aatgatcgca gccgcggcgc ttcaggaaga cgtcgacgcg gtaggcgtct cgatcctgag
      841 cggcgcacac aacacccttt gcccgcgcat cgttcagttg cttcgtgaaa aagggatgga
      901 caactgcctg gtcctggtgg gcggcatcgt tcctcaggaa gacgttccca agctcaagca
      961 acagggcgtt gccgaggtct tcctgcccgg tacctcaacc ggagacatcg tcaagtttct
     1021 aagcgataac gtcaaaccgc gcgagtaaac cttcgcattc ccggcacctt tttcctggga
     1081 ccgcaggcgt ctcgcctgct actcacaaaa gcgccactac cgtttccgag caggcgagac
     1141 gcctacgctc ccagaaaaga agctctctat tttttggtcc tatttagaaa cccggcgact
     1201 tgttcctggc aatctttggt gagtctggcg atcacgttaa cgtcggcgcc tgtttccaat
     1261 gcggccgaaa atgtgagccc gtccatttgg tagagcagcg tcttcgtgag ggatattgct
     1321 gagcggctta gcttcgaaaa ttcgtttaga taggccgtga cgtcatcgtc gaatgtttca
     1381 tccgcgaaaa tgtgattcac caggccagac tccagcgctt gttcggcact gatttcagct
     1441 ccgcgggtga ggagttcaaa agcaatcttc tccgaaacgt tgcgccgcag gatcgccatc
     1501 accattgctg gcacaaaccc gatcttcact tccgggtaac caaaacgcgc agacgcagac
     1561 gccagcacga tatcgcacgc gcttgccaga ccgcagccgc ccgctagcgc ccggcctcgt
     1621 accgctgcca ccaccggaac agggatctga cgtatcaata cgaacagatc tagtaacgag
     1681 cgtgcatcct ccgcgttctc agaaactgaa gcctgagcga tcgtctgtag tgccgataga
     1741 tcggcgccgg aacaaaaatc ctttccggcg ccgctgatca tgacaactct gacgcgttcg
     1801 tctttgccgg cagcgtgtag tttctctttg agacccgcga caagcgcggc attcaacgca
     1861 tttctttttt ccggtcgatt gagttgtatg cgcgccaagg cgccttcaac cgaatagagg
     1921 actggttgtt cgtcggccat tcagttcaac tcgagctttc gcgcttcttc atcggagacc
     1981 tcaatctcca tccgcaacaa cgccgtggaa aacgtcttac cctgcgcgtc agtcatcaac
     2041 gacagcgttc cacccccgcc gagactctca tgcagcagga aattcaacgc gcccagattt
     2101 ggcaattcga atcgctccac ttcaccacgg cagatgccgg tgaaatgctt cttgactcgc
     2161 tcagccgtaa cttcacgaac caggatcggg tagacctctt cgcgtaaggc tatcaatccg
     2221 acattcgcgg tgtcgccctt gtcgccggat cttgcgtggg ccagatttgt cagttgcact
     2281 ttcacgcttc gagcacctca atttcggtca tgatctcctt tttagggata agcgcaggcc
     2341 aataggcgac gatctcttcg accttaggac ggccgcccgc aaatcctgtg actgccggtg
     2401 gacccgtcag aattaacggc gcgatctctt tggtaaatcg ctcgatcgcg ccgcggtcct
     2461 gtccgcgtac gccaaaccgc agttgcacct ccggcagatc ggcgcgaggc tcgccggcta
     2521 atggaccatg cgtcgcgtta acaccgacaa actccgtcag cacgtagtcg aactgcagac
     2581 ccaagcgatc c
//
LOCUS       DASTEY010000344         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_765725, whole genome shotgun sequence.
ACCESSION   DASTEY010000344 DASTEY010000000
VERSION     DASTEY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765725"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFO72_06065"
     CDS             complement(<1..301)
                     /locus_tag="VFO72_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478888.1"
                     /translation="MNITHLECGLCGLKHEARRLLNLCRDCGKPLLVRYDLERAARTL
                     TKDSLPARRADMWRYREVLPVENEENIVSLGEGWTPLLRAANLGERVGVKELYIKD"
     gene            complement(403..939)
                     /locus_tag="VFO72_06070"
     CDS             complement(403..939)
                     /locus_tag="VFO72_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET9478889.1"
                     /translation="MRRLGVSIAVIALVSASTVVLAQGGFKRISEFLTGYEETPSAVS
                     TTGNGTFKARISNDGSQIEWELSYADLEGAVQQAHIHFGQKSVTGPITVFLCTNLGNG
                     PEETQPCPAPPATISGTITAADVTAGAADKGIDAGELDELIDAIRAGATYVNVHSTRW
                     PTGEIRSQIDGNSGHGGH"
     gene            1041..1901
                     /locus_tag="VFO72_06075"
     CDS             1041..1901
                     /locus_tag="VFO72_06075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET9478890.1"
                     /translation="MAISLLALDLDGTLLNNRGHISERNLAAINSARAKGVRVALVTG
                     RRFRDSRPVALELGLDIPLISHNGALTKHAETLQTVAVLPLPLDAAREALRVGRQAGA
                     DALLSDDHEGLGVLVYDHLSGDNVAVHRYIAWAIRLHGAEEGRNAVRQVDSLENYLDH
                     EPIHLAFSGRCEAMDQLADLLNSELGSGVKILSTKYLEQDFTLIDVMNPAASKGAGVA
                     AAATELSIAAEEIMAIGDNYNDLEMLLFAGTGVVMANAPLSLREIPGLHPTASNSEDG
                     VALAIEQFIL"
     gene            1919..2401
                     /locus_tag="VFO72_06080"
     CDS             1919..2401
                     /locus_tag="VFO72_06080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9478891.1"
                     /translation="MANRTAVIQTNKGTIRAELLESDAPKTTENFITLAQRGYYDGII
                     FHRVIKGFMIQGGDPTGTGRGGQSAWGGRFDDEIDPKSEVYQRGYKAGTMAMANAGPN
                     TNGSQFFIMHVDYPLPPSYTIFGRVVEGQDVVNAIATSATDRNDRPLEEVRMEKVTIE
                     "
     gene            complement(2391..3131)
                     /gene="thiD"
                     /locus_tag="VFO72_06085"
     CDS             complement(2391..3131)
                     /gene="thiD"
                     /locus_tag="VFO72_06085"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET9478892.1"
                     /translation="MVLTIAGIDPSGGAGIVADIKTIAAFGCFPAAAITSITFQNAKR
                     VFGAEHQTAATLRAQVEPIVQDVTVVAAKTGMLPTAEIVAEVVRLFREQKLPAPVVDP
                     VMVSTSGHDLIGEEAFQILKTELLSVARLVTPNIPEAERLTGFSLHTEADMKRAAEAI
                     KSLGARAVLVKGGHRLDAQADAVDILIDDTGAIAEFREEYIDVGEVHGSGCTLSAAIA
                     AGLGKGLSLEDAVGAAKKYLTDQIRLIR"
     gene            complement(3142..>3713)
                     /locus_tag="VFO72_06090"
     CDS             complement(3142..>3713)
                     /locus_tag="VFO72_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET9478893.1"
                     /translation="TSGATTSRTTRDDYEFVRILQLAEAAVVAQIPLFQIREKSLRGR
                     VLYELVTEAVKTTRGSASRVLVNDRFDIARAAGADGVHLTSLSLPVHVVREACGPEFL
                     IGASTHSLETAREARDGGADFVVFGPVFETESKHVYGPPQGLEKLQRVTRALQGFPVL
                     AIGGVNLDNADRCFAAGASGFAGISWFNR"
BASE COUNT          823 a         1062 c         1023 g          805 t
ORIGIN      
        1 catcttttat gtaaagttct ttgacgccaa cgcgctcgcc taagtttgca gcgcgaagca
       61 gcggcgtcca accttctcca agtgaaacga tgttttcctc gttttcaacc ggcagcacct
      121 ctcgatagcg ccacatatcc gcccggcgag ccggcaaaga atccttagtc aaggtgcgcg
      181 cggcgcgctc caaatcgtag cgaacgagta atggcttgcc gcagtcgcga caaaggttga
      241 gcaaccgacg tgcctcgtgc ttcagcccgc agaggccgca ctcaagatgg gtaatgttca
      301 tgggttttta ctacggcagg actagacaat atgcaagggg tcacgtgctg gatgcagtaa
      361 accccttgca ctcggagcaa aaaacttctg tcgggatgct gcttagtggc cgccgtggcc
      421 gctgttacca tcgatctggc tgcgaatttc gccagttggc catctcgtgc tgtgaacatt
      481 cacataagta gctccagcac gaatcgcgtc gatcagttca tcaagttctc cggcgtcaat
      541 acccttgtcg gctgccccgg ccgtgacgtc cgccgcggtg atcgtgccgg agatagtcgc
      601 tggtggcgcg ggacaaggct gtgtttcttc cgggccgttg ccgaggttcg tgcaaaggaa
      661 cacggtgatc gggccggtca cagacttttg tccaaagtgg atatgagcct gctggacagc
      721 gccttcgaga tcggcgtaac taagctccca ctcgatttgc gatccatcgt tgctgatgcg
      781 cgccttaaaa gtcccattac cggtggttga gaccgctgaa ggagtttcct cgtatccagt
      841 taaaaactcg ctgattcttt tgaagccacc ttgggcgaga acgacagtcg acgccgatac
      901 caaggcgatt actgcaatag acactcctag tcttcgcatc ccttttacct ccagaacttg
      961 agttgttgtt taatgggggc gcgcatcata tcacagccag tttgatctag gtatccgtga
     1021 atgctaattt gtcggtcttt atggcgataa gtctgctggc gctagatctc gacgggacac
     1081 tcctcaataa ccgcggtcac atttctgaac gcaacctggc ggcaatcaat agtgcacgtg
     1141 cgaaaggtgt tcgtgtcgcg ctcgtcaccg gacgccgctt tcgtgattcc cggcccgtcg
     1201 cgctcgaatt aggtcttgat attcctttga tctctcacaa cggcgcgtta accaaacatg
     1261 ccgaaacttt acaaacggtc gcggtgctac cgcttccact cgacgccgct cgcgaagcgc
     1321 tgcgcgtcgg acgtcaagca ggcgccgatg cgctattgag cgacgatcac gaagggctgg
     1381 gcgtgctcgt ttacgatcat ctcagtggcg ataacgttgc cgtccaccgg tacatagcct
     1441 gggccatacg gcttcacggc gctgaagaag gacgaaacgc cgtgcggcag gttgactcgc
     1501 tggagaacta cctcgatcat gaacccattc atctcgcgtt ctcaggacgt tgcgaagcga
     1561 tggaccaact cgcggacctg ctcaactccg agttgggatc cggtgtgaaa attctgagta
     1621 cgaaatatct tgaacaggat ttcacactca tcgacgtaat gaatccggct gcttcgaagg
     1681 gggcgggagt cgcggccgcg gcaacggaac tgagtatcgc agccgaagag atcatggcga
     1741 taggcgacaa ctacaacgat ctggaaatgc tacttttcgc cggcaccggc gtggtgatgg
     1801 caaacgcgcc gctatcgttg cgtgagattc ccggcttgca tccgacggcg tccaacagtg
     1861 aagacggtgt ggcgttggca atcgaacaat tcattctgtg accagcaagg agacaaacat
     1921 ggcaaatcga acggcagtga ttcagacaaa caaaggcacc attcgcgccg agctgttgga
     1981 atcggacgca ccgaagacca ccgagaactt catcacgttg gcccaacgcg gttattacga
     2041 cggcatcatc tttcatcgcg tgattaaggg tttcatgatt cagggcggag atccgaccgg
     2101 caccgggcgc ggtgggcagt ctgcgtgggg cggacggttt gatgatgaga tcgatccgaa
     2161 gtcggaggtg tatcagagag gttacaaggc cgggacgatg gcgatggcca atgctggtcc
     2221 taacacgaat ggatcgcagt ttttcatcat gcacgtggat tatccgctgc cgccgagtta
     2281 cacgatcttt gggcgcgtgg ttgaagggca ggacgtagtg aacgcaatcg ccacgtcggc
     2341 cacagatcgc aacgatcgtc cgcttgaaga agtccggatg gagaaggtga ctatcgaata
     2401 aggcggatct gatccgtgag gtactttttg gcggctccga ctgcgtcctc gagcgacaat
     2461 ccctttccca gaccagcggc aatggcggcc gacagggtgc agccggagcc gtggacttca
     2521 cccacgtcga tgtactcctc gcggaactcg gcgatcgcgc cagtgtcgtc gatcagaata
     2581 tccactgcgt ccgcttgcgc gtccaatctg tgtcctccct tgactaacac tgcacgagcg
     2641 ccgagagatt ttattgcctc ggctgctcgt ttcatgtctg cttcggtgtg aagggaaaac
     2701 ccagtcagac gttccgcttc gggaatattg ggcgtcacca gacgagccac ggataagagc
     2761 tccgttttca agatctgaaa agcttcctca ccgatcaagt cgtggcctga ggtcgaaacc
     2821 attaccggat ccacgaccgg tgctggtaat ttctgttcac gaaacagacg caccacttca
     2881 gcaacaattt cagccgtcgg caacatcccc gtcttcgccg ctacgaccgt cacatcctgc
     2941 acaatcggct caacctgcgc gcgcaaagtt gccgctgtct gatgttccgc accaaaaaca
     3001 cgtttcgcgt tttgaaaagt gatcgacgtg atcgccgccg cgggaaaaca gccgaacgcc
     3061 gcaatggtct tgatatccgc aacgatgcct gcgccgccgg acggatcgat cccggcgatt
     3121 gtgagcacca ccggtttatc gttatctatt gaaccaactg attcccgcaa aaccactcgc
     3181 tcccgctgca aaacatctat ccgcattatc aaggttgacc ccgccaatcg ccagcaccgg
     3241 aaacccctgc aatgcacggg tcacccgttg aagtttttct aacccctgcg gcggaccata
     3301 gacgtgcttc gactcggttt caaatactgg accaaacacg acaaagtcgg cgccgccgtc
     3361 acgagcttcg cgcgcggtct ccagcgagtg tgtggaagca ccgatcagaa actccgggcc
     3421 gcatgcttcg cgcaccacgt gcaccggcaa cgaaagtgac gtgagatgca cgccatcggc
     3481 gcctgcggcg cgtgcgatgt cgaaacgatc gttgaccagc acgcgcgatg cactgccgcg
     3541 agtagttttc accgcctcgg taacgagttc gtaaagaact cgtccacgca aagatttttc
     3601 gcgaatttga aaaaggggaa tctgcgccac taccgcagct tcggccagtt gcaggatgcg
     3661 cacgaactcg tagtcgtcac gagttgttct gctcgtcgtt gcgccgctcg tta
//
LOCUS       DASTEY010000345         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_89930, whole genome shotgun sequence.
ACCESSION   DASTEY010000345 DASTEY010000000
VERSION     DASTEY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89930"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFO72_06095"
     CDS             <1..1415
                     /locus_tag="VFO72_06095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9478894.1"
                     /translation="PSSMALGRNLFIFSNHLLFLIGSGLFNVQPENAYLIFKYAVVAQ
                     APLAIVACWILARDISGSLYTATIAALLITLSPVFVLYGGQVMTDVPSVLLLATALVV
                     HLRGVRKKKLWLILLGAALLGLGVNLRETIGFYFPWLLLAPFVLGWKFEKREVLYVVA
                     SCVVFVVLAFGWFGYWFLTDPHYRWVWNGWRESMREESARHPVEFANLRPYLILYFIS
                     APLVFLTIWFAPVFEWRKRRMTPLLLLWLVGFFANVLLFFNYSTTVNWRYFLTGLPAI
                     APLGAFWLLRIAETSLGNVRRAFIACTGLIVLVAVILFIYMRPISFEFIERRAMSKEY
                     KHRLEHVPTDAVMISGSQTIAVHYWAAIGDGDWKAIGTGGGWPGDRLFSMVKNYLKEG
                     RPVFIDIDPRWWLPCGWQREEIPTIVRLEEHFSFRRVSETIYELRPLGDSDATDHPNL
                     KRLLPENRPEDTKKCPPARN"
     gene            1446..2663
                     /locus_tag="VFO72_06100"
     CDS             1446..2663
                     /locus_tag="VFO72_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478895.1"
                     /translation="MPYEVFLAFRYLRSRPKRRLARVTALLAILGIAMGVAALIVALA
                     LGNGFRDEMRDKILQGTAHLTVLRSDGSAIEDYVNVTERLRRVEGVVSASATTYDGAL
                     ARGPKGSGYAVLRGIEKGNEAQVQRWLIEGGTGPLFDAAGTGERAIPPAAIGAELAAK
                     LGVAVGDAFAILPANDLTGSQIRHLRVAGVFRSGLFEYDSTWIYVAFETATSLARGEH
                     AGSVISVQVNDAENVKQIAARVLGTLGDGYTVVDWQQANQPLFAALALERRMGVFIIG
                     LIVAIAALNITTMLILVVVERRRDIAILSTLGATRAGVMLVFIIEGAVVGAVGAIAGV
                     MLGLAACFLGNHYKLVSLPPDVYSISNVPLHANFSETVLAALIAFGLSVLATIYPARN
                     AARMRPVEVLREV"
     gene            2663..3283
                     /locus_tag="VFO72_06105"
     CDS             2663..3283
                     /locus_tag="VFO72_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478896.1"
                     /translation="MRLEVVDLRKSFAAPNGGRIEVLRGVSFAVNAGECFAITGPSGS
                     GKSTLLHLVGGLEEADHGSLTAFRREQIGFVFQFHYLLPDLTAAENVALPLLIRRSGK
                     AYQQAKALLAETGLRDRLDHRISHLSGGEQQRVAVARALITEPQLVLADEPTGNLDPA
                     IGDEIGRTLVSYAKTRPAIVIIATHSPSLAQLCDRVMFLEGGRLKP"
     gene            3388..>4722
                     /locus_tag="VFO72_06110"
     CDS             3388..>4722
                     /locus_tag="VFO72_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET9478897.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIEPEHLLLGLMREDKTL
                     TGRFFPRAQVSIESIRKEIEGRTLLREKISTSVELPLAPETKHVLAYAHEESDRLQHR
                     HIGTEHLLLGLLREERSMAAEILYERGLRLNAVRDEIARQSGADARSTQKKDTPHLVE
                     FSRDLTEDAANDKLDPLIGREAEIERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIVRGDVPSFLENKRILSLDLSLIVAGTKYRGQFEERLKQIMRELVENPQYIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PPPSEAEAVSILDGVRERYESFHQVRYTDDALQAAVYQSHRYIPDRFLPDKAIDVIDE
                     AGARVKLRVRREQGNLADWNELNEWSRSYGDSASLRMTHEDALVSAEVTRDDV"
BASE COUNT          942 a         1229 c         1449 g         1102 t
ORIGIN      
        1 acccgtcgtc aatggcgctg ggccgtaacc tgttcatctt tagcaatcat ctgttgtttt
       61 tgatcggcag cggcctattc aacgtgcagc cggagaatgc gtacctgatc ttcaagtacg
      121 cagtggtggc ccaggcgccg cttgcgatcg tggcgtgctg gattttagcg cgcgacatca
      181 gtgggtcgtt gtacacggcg acgattgcag cgttgctgat cacattgtcg ccagtgtttg
      241 tgctttacgg cggacaagtc atgaccgacg tgccgtcagt gctgctgctg gcgacggcgc
      301 tggtggtcca tctgcgtggg gtgcggaaaa agaaactgtg gttgattttg ctgggcgcag
      361 ctttgctggg actcggcgtc aatctgcgcg agacgatcgg gttctacttt ccgtggctgt
      421 tgctggcgcc tttcgttctt ggctggaagt ttgaaaaacg cgaggtgctt tacgttgtcg
      481 cttcgtgtgt tgtgtttgtt gtgctggcat tcggttggtt tggttactgg tttcttacgg
      541 acccgcacta tcgctgggtg tggaatggct ggcgtgaatc gatgcgtgag gagtcggcgc
      601 gacatcccgt ggagttcgcc aatcttcgtc cttatttaat cctgtacttc atcagcgcgc
      661 cgctcgtgtt tctcacaata tggttcgcgc cggtgtttga gtggcgcaag cgccgaatga
      721 cgccgctgct gttgctgtgg ctggttgggt ttttcgcgaa cgtgcttctg tttttcaact
      781 acagcaccac tgtcaactgg cgttactttc tcaccggctt gccggcgatc gcaccgttag
      841 gtgctttttg gctgctgcga atcgcagaga cctctctcgg aaacgtgcga cgggctttta
      901 tcgcttgcac gggcttaatc gtgttggtgg cggtgatttt gttcatttac atgcgcccca
      961 taagtttcga gttcatcgag cggcgggcga tgagcaagga gtacaaacat cgactcgagc
     1021 acgtgcctac tgatgccgtg atgatttcgg gttcgcaaac gattgcggtg cattactggg
     1081 cggcgattgg cgatggcgat tggaaagcga tcgggacggg cggtggctgg ccgggcgata
     1141 gattgttttc gatggtaaag aactatctaa aagaagggcg gccagtcttt atcgatatag
     1201 atccgcggtg gtggttgccg tgtggttggc aacgtgagga gatcccgaca atcgtgaggc
     1261 tggaggagca ttttagtttc cgccgtgtca gcgagacgat ttatgagttg cggcctctgg
     1321 gagattctga cgcgacggac catccgaatt tgaaacggtt gctgccggaa aaccggcctg
     1381 aagatacgaa gaagtgtccg cccgcacgca attagatcac ggattacacg gactagttaa
     1441 cacagatgcc gtatgaggtt tttctggcgt ttagatattt gcggtcgcgg ccgaagcgcc
     1501 ggctcgcgcg cgttacggcg ttgctggcca tactcggtat cgcgatggga gttgcggcgt
     1561 tgattgtggc gctggcgttg gggaacgggt ttcgcgacga gatgcgcgac aagattttgc
     1621 agggcacggc gcacctcact gtgttgcgga gtgatggaag cgcgattgag gattacgtga
     1681 acgtgaccga gcggttgcgg cgagttgaag gggtcgtgag cgcgtcggcg acgacgtatg
     1741 acggtgcgct tgcgcggggc ccgaaaggtt cggggtatgc ggtgttgcga ggcatagaga
     1801 agggaaatga ggcgcaagtg caacgttggt tgattgaagg cggaactgga ccgctgtttg
     1861 acgcggcggg cacaggcgaa cgggcgatcc ctccggcggc aataggggct gaacttgctg
     1921 cgaaacttgg agtagcggtc ggcgatgcct tcgccatatt gccggcgaac gatctcaccg
     1981 gcagccagat tcgacactta cgcgttgcag gtgtttttcg ctcgggtttg tttgaatatg
     2041 actcgacttg gatctatgtc gcctttgaga ccgcgaccag tcttgcgcga ggcgaacatg
     2101 ctggttctgt aatcagtgtt caagtcaacg acgcagaaaa cgtaaagcag atcgctgcgc
     2161 gcgtgcttgg cacactcggt gacggttaca cagttgtcga ttggcaacaa gcaaatcaac
     2221 cgctcttcgc ggcgcttgca ctcgagcgtc gcatgggcgt gtttattatc ggactgatcg
     2281 tcgcgatcgc ggcgctcaac atcaccacca tgttgattct cgtcgtcgtc gagcggcggc
     2341 gtgacattgc gatactcagc acactcggcg caacgcgcgc gggtgtgatg ttggtgttca
     2401 ttattgaagg cgcagttgtc ggcgcggttg gcgcgattgc gggagtgatg ctcggactcg
     2461 ccgcctgctt cctcggcaat cattacaaac tcgtcagcct gccgccagac gtctactcga
     2521 tcagtaacgt gccgctgcac gccaacttca gcgaaacagt tctcgccgcg ctgatcgcgt
     2581 ttggtctaag cgtgcttgcc acgatctatc ccgcgcgcaa cgcggctcgc atgcgaccag
     2641 tcgaggtcct acgggaagtc tgatgcgttt ggaagtcgtc gatctgcgaa agagctttgc
     2701 cgcgccgaat ggaggccgaa tcgaagtgct gcgtggtgtt tcattcgcag taaacgcggg
     2761 tgagtgtttt gcgatcactg gtccttccgg ctcaggaaag tcgacactgc tgcatctcgt
     2821 tggcgggctg gaggaagcgg accacggatc cctcacggct tttcgccgtg agcagatcgg
     2881 gtttgttttt cagtttcact atctcttacc ggacctcaca gccgccgaaa acgtggcgct
     2941 gcccttactg atcagacgtt ccggaaaggc ataccaacag gcgaaggcgc ttttagcgga
     3001 gacgggattg cgagaccggt tggaccatcg aatctcgcat ctctcaggcg gcgaacaaca
     3061 gcgggtcgcc gtggcgcggg ccttaatcac agaaccgcag ctcgttttag cagacgagcc
     3121 gactggcaat ctcgatcctg cgattggcga tgagatcggc cgcacgctcg tcagttatgc
     3181 aaaaactcgg cctgctatcg tcatcatcgc aacgcacagt ccgagtttgg cacaactttg
     3241 tgaccgagta atgtttttgg aaggcggacg gttgaagccc tgaaatagcg gctgcgttgc
     3301 gtctttccat aggttataat gcgcgcagtt gccgggcaga ggttttgccc ggcttggtaa
     3361 tcataaggac ttttacgtta aaacaggatg ttcgaacgct acactgaaaa ggcgcgccga
     3421 gttattttct tcgcgcgtta cgaggcttcc cagtttggag cgccggcaat tgagccggag
     3481 catctgctgc tggggctgat gcgcgaagat aaaaccctga cgggccgctt tttcccgcga
     3541 gcacaagttt ccatcgagtc gattcgaaag gagatcgaag gccgcacgtt actgcgcgag
     3601 aagatctcaa cttcggtcga actcccgctc gctcccgaaa ctaaacacgt tcttgcttac
     3661 gcccatgaag aaagtgaccg gctgcaacac cgccacatcg gaaccgaaca cctgttgctc
     3721 ggtttacttc gcgaagagcg ttcgatggca gccgagattc tttacgaacg tggactgcgt
     3781 ttgaacgccg tgcgcgatga gatcgcgcgg caatcaggcg ccgatgcgcg cagcacacaa
     3841 aagaaagaca cgccgcacct cgtcgaattc tcgcgcgatc tgactgaaga cgctgccaat
     3901 gacaagttgg acccgctaat tggacgcgag gctgagatag aacgcgtcgt gcagattctt
     3961 tgccgtcgca ccaagaacaa ccccgtcttg atcggtgagc ctggcgtcgg caagaccgcc
     4021 atcgtggaag gtctcgcaca acgcatcgtg cgcggtgacg tcccgtcatt cctcgaaaac
     4081 aaacgcatcc tttcgctcga cctgtcgctg atcgttgccg gtacaaaata ccgcgggcag
     4141 tttgaagaac gtttgaaaca gatcatgcgc gaactcgtcg aaaacccgca gtacatcgtt
     4201 ttcattgacg agttacacac gctcgttggc gcaggctcgg cggaaggttc acttgacgcg
     4261 gccaacattt tgaaacccgc tttgtcgcgc ggcgagattc aatgcatcgg cgcgaccacg
     4321 cccgcggagt tccgcaagtc gatcgaaaaa gatcgctccc tcgaaagacg ttttcaggca
     4381 gtgaaggttc cgcctccatc cgaagcggaa gcggtcagca ttctcgatgg cgtgcgcgag
     4441 cggtacgaaa gttttcatca ggttcgttac acagacgacg cgctgcaagc tgcggtttac
     4501 cagtcgcatc gttatattcc cgaccgtttt ctccctgaca aagccatcga cgtcattgac
     4561 gaagcgggcg ctcgcgtgaa gctgcgcgtt cgccgcgaac agggaaatct tgccgactgg
     4621 aacgagctga acgaatggtc gagatcttac ggcgacagcg cgagcttgcg catgacgcac
     4681 gaagacgcgc tggtttccgc ggaagtcacg cgcgacgacg tt
//
LOCUS       DASTEY010000346         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_600586, whole genome shotgun sequence.
ACCESSION   DASTEY010000346 DASTEY010000000
VERSION     DASTEY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600586"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..870)
                     /locus_tag="VFO72_06115"
     CDS             complement(97..870)
                     /locus_tag="VFO72_06115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9478898.1"
                     /translation="MNATLITGASSGIGAAFARKLAKRGRNVLLVARSEDKLIALCNE
                     LGRVSGIRAQYVVMELTEDDAPARLFEETHKRELQIDMLVNNAGFGSMGDFGRLSLAR
                     ELEMIDLNVKSLVALTHRFLGPMRERRQGTIINVASTAGFQAVPFMATYAASKAFVLS
                     FSEALWEENRPHGIHVMALCPGVTDTNFFAASKIDRPPMRTVQTAEEVVDAALRGLTH
                     GKSVVISGWTNWLTVEAERFVPRSFVTKLAAKALRSRFE"
     gene            complement(867..2078)
                     /locus_tag="VFO72_06120"
     CDS             complement(867..2078)
                     /locus_tag="VFO72_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HET9478899.1"
                     /translation="MKVLVADKFEQSGRDGLQALGCDVSFQPDLKDDALVNAITDVAP
                     DVLVVRGTKVTEPMLAAGPIKLVVRAGAGYNTIDVAAASKRGIYVSNCPGKNSIAVAE
                     LAFALILALDRRIVDNVVTLRRGEWNKKEFSKARGLFGRTLGLIGVGRIGQEMVTRAR
                     AFGMPVIAWSRSLTPLRAAELGITHKESPKAVAAEADIVSVHLALNSETRNAIDADFF
                     SVMRPGAYFINTARGEVVDQQALVNALRTRGIRAGLDVYAVEPTSGVGEFNDDVTKEE
                     GLYGTHHIGASTDQAQEAIAAETVRIVKQFKETGHVPNVVNLARQTPATHRLVVRHVD
                     RPGVLAGVLDAIKSEQINVQEMENIVFEGAAAAVARINLDNAPSDAILNRIRSGSADI
                     IELDLIQLGSQ"
BASE COUNT          448 a          644 c          575 g          482 t
ORIGIN      
        1 gttaattatc gcgtgcgttt ccgtgtctct gtgtctcggt ggttgatacc gagcgaagtt
       61 tgaccaccga gacacggaga cacagacagg aagccgttat tcaaatcggg atcgcagtgc
      121 cttggcggcc aatttagtca caaacgatcg tggcacgaat cgttcggcct caacagtgag
      181 ccagttggtc caacctgaaa ttaccacact ctttccgtgc gtaagcccgc gcagcgccgc
      241 gtccacaact tcttccgccg tttgtaccgt ccgcatcggc gggcgatcga ttttcgaggc
      301 cgcaaagaaa ttcgtatccg ttacacctgg acacaacgcc atcacatgaa ttccgtgcgg
      361 acgattctct tcccaaagcg cttccgaaaa cgacagcaca aacgctttcg acgcggcata
      421 tgtcgccata aatggcaccg cctgaaaacc ggccgtcgac gcaacgttga tgatcgtgcc
      481 ttgtcgtcgt tcacgcatcg gaccgagaaa ccggtgtgtc aacgcgacta aagacttaac
      541 attcaggtcg atcatctcga gctcacgcgc gagactgagt ctcccaaagt cgcccatcga
      601 gccgaaaccg gcattgttga cgagcatatc gatctgcaat tctcgtttgt gagtctcctc
      661 gaacaaacgg gccggcgcgt catcctcggt caactccatc acgacgtatt gcgcgcggat
      721 gccgctcacg cgcccgagtt cgttacacaa cgcgatcagt ttgtcttcgg agcgagcaac
      781 caacagcaca ttccggccgc gctttgcaag ctttctggcg aaggctgcac cgatgcctga
      841 agacgctccg gtgatgagag tcgcgttcat tgactaccta actgaattag gtccaactcg
      901 ataatgtcgg cgctgccgga tcgtattcga ttaagaatcg catccgacgg cgcattgtcg
      961 aggtttatgc gcgccacggc agcagcagcg ccttcaaaga ctatattctc catttcctga
     1021 acgttgatct gctctgactt gatcgcgtcg agcacgcccg caagcacacc ggggcgatcg
     1081 acgtgacgga ccactagccg gtgcgttgcg ggagtttgcc gcgcgagatt gacgacgttc
     1141 ggcacgtgtc cggtctcctt aaactgtttc acgatgcgca ccgtttcggc cgcgattgct
     1201 tcctgcgcct ggtccgttga agccccgatg tgatgtgtgc cgtagagtcc ttcttctttg
     1261 gttacgtcgt cgttgaactc gcccacgccg cttgtaggtt caactgcata aacgtccaac
     1321 ccggcgcgaa tgccgcgcgt tctcagcgcg ttgaccagcg cttgttgatc gacaacctcg
     1381 ccgcgcgcag tattaatgaa gtaagcgcct gggcgcatca cactgaagaa gtcagcgtcg
     1441 atcgcatttc tcgtctcgct attgagtgcg aggtgaacac tcacgatgtc agcttccgcg
     1501 gcgacggctt tgggcgactc cttgtgcgtg ataccgagtt ccgccgcacg cagtggcgtc
     1561 aggcttctgc tccaagcaat gacgggcata ccaaacgctc gtgcgcgcgt gaccatctcc
     1621 tgtccgatcc gaccgacgcc gatcagtccc agcgttcggc caaacagacc acgcgctttg
     1681 gaaaactcct tcttgttcca ctcgccgcgc cgcagtgtga caacgttgtc gacaatgcgg
     1741 cggtcaagcg cgaggatcag tgcgaaggcg agttcggcaa cggcgatcga gtttttgccg
     1801 ggacagttcg aaacgtaaat gccacgtttc gaagcggctg ccacatcgat tgtgttgtaa
     1861 ccggcgcctg cgcggacaac gagtttgatg ggaccagcag ccagcatcgg ctcagtcact
     1921 tttgtgcctc tgaccaccaa cacgtccggc gccacgtctg taatcgcgtt tacgagcgca
     1981 tcgtctttca agtccggttg aaacgaaacg tcgcaaccga gcgcttgcag gccgtcgcga
     2041 cctgactgtt cgaatttatc ggctactaag actttcatcg cattagattg tgtgaatcag
     2101 taatccgtcg cggagcttcg gttcgaacca cgtcgacttc ggcggcatg
//
LOCUS       DASTEY010000347         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_666798, whole genome shotgun sequence.
ACCESSION   DASTEY010000347 DASTEY010000000
VERSION     DASTEY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666798"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFO72_06125"
     CDS             <1..420
                     /locus_tag="VFO72_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9478900.1"
                     /translation="GRRVLVIVSDGIETTSQHTDFDFVVHRVLADDCQVYVVQTGLYE
                     GANLRALAAERRMEQLTSQTGGAVYLPKTTAQLDIAFDQIAADLAHQYVLSYYPGPEN
                     RDGRLHLLDLRLKTRNDVRVRSRKGYYAPKPAASAGW"
     gene            597..1511
                     /gene="xerC"
                     /locus_tag="VFO72_06130"
     CDS             597..1511
                     /gene="xerC"
                     /locus_tag="VFO72_06130"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET9478901.1"
                     /translation="MEKLVEQFLEHLRYERNVSAHTLRNYSSDLEQFLDFLDPSGKRP
                     RTLPDVTEIDHLTIREWLAQLHSAQKKKASIARKLAALRTFFQFLVREGIVELNPAKL
                     VATPRLEKKLPAHLSIEEAIRFIESPDLETDLGKRDRAMLELMYATGVRVAELTTLNV
                     SDVDLHNRLIRVTGKRRKQRIVPFGDPAGDALRGYLAVRGKFLLNAPVSKRDEEVLFL
                     NYQGTRITTRSVGRMVEKYIRLCAGMHNISPHALRHSFATHLLDSGADLRDIQELLGH
                     ARLSTTQIYTHVSMEKLVEVYDKSHPKA"
     gene            complement(1534..1971)
                     /locus_tag="VFO72_06135"
     CDS             complement(1534..1971)
                     /locus_tag="VFO72_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HET9478902.1"
                     /translation="MSRIHCRTRLATNAILAPTKTMDAEKLKHFRSLLLNQLRQYTEQ
                     IRTDQAAALDLYDDDVKDSVDLSVQDLSKEIALRLGERSSQVVADIDQALLRMKEGSY
                     GICARCSRPIDERRLEALPTARYDAACQAIIESANGEEDLPTL"
     gene            complement(1978..2937)
                     /locus_tag="VFO72_06140"
     CDS             complement(1978..2937)
                     /locus_tag="VFO72_06140"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478903.1"
                     /translation="MPSIYEAEPLELKNVRNYPLASRTSKVSLENFARPLSDAASVEE
                     FIASLPDVLAVQALRKIVERMRRARELEKPIIWGLGGHVIKTGLAPVILDLMERGYVS
                     AIAANGSVLVHDAEIAMIGSTSEDVDATLSEGIFGGADETGQLLNRAAQDGAADRIGL
                     GEAVGRNLLAMQPQHEQYSLLCGAYKTRVPFTIHLTIGGDIAHFHPSADGAALGATTH
                     TDFRLLAELVRRMDGGGVYLNIGSAVVLPEVFLKCVTLVRNLGHPLSKITTANFDFIQ
                     SYRPLTNVVRRPTEKGAGEGHSITGHHELTIPLLAAMLIAHTG"
     gene            complement(2956..3396)
                     /locus_tag="VFO72_06145"
     CDS             complement(2956..3396)
                     /locus_tag="VFO72_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478904.1"
                     /translation="MGTIQLPPDFKEFLRLLNSRNVEYLIVGGYAVGYHGYPRPTGDL
                     DIWISRTRENAERALGALQEFGFSCPAELLQQDNQLARMGIAPFRIEIMTTIDGVNFA
                     ECYVERVHGELDGVEVNMISLPRLRENKKASGRSKDIMDLENLP"
BASE COUNT          788 a         1027 c         1012 g          855 t
ORIGIN      
        1 ggccgccgcg tgttggtgat cgtctcagac ggaattgaaa cgacgagtca acacaccgat
       61 ttcgatttcg tggtgcatcg cgtgctcgcc gatgattgcc aggtctacgt ggtccaaacg
      121 ggcttgtatg aaggcgctaa cctgcgcgcg cttgcggccg agcgacgcat ggaacagttg
      181 acctcacaaa ccggcggcgc agtctacctg ccaaagacaa cggctcaact cgatatcgct
      241 ttcgaccaga ttgccgccga tcttgcgcac cagtacgtgt tgagttatta cccgggtccg
      301 gagaatcgcg acggccgttt gcacctgttg gatctgcgac tcaagacgcg aaatgatgtt
      361 cgcgtgcgct cgcgaaaagg ctattatgct ccgaagccgg cggcatctgc cggttggtga
      421 tgcagtttga aactgaggcg tcttcctcct gggaccgcag gcgtctcgcc tgcacggtga
      481 cggatcgtgc gctgtcgtga gatgcaggcg agacgcctgc ggtcccaggt aaacaagccc
      541 cctgtttcaa accgcatcag tgctagtcgt caacgacgcg ctgctagacg tttttaatgg
      601 aaaagcttgt cgagcaattc ctcgaacacc tgcgttacga gcgcaacgtc agcgcccaca
      661 ccttacgcaa ctactccagc gatctcgaac agtttctcga ttttttggac ccaagcggaa
      721 agcggcctcg tactcttcct gatgtaaccg agatcgacca cctcaccatt cgcgagtggc
      781 ttgcacaact tcatagcgcg caaaagaaga aagcctccat cgcacgcaag ctggccgcgt
      841 tgagaacttt ctttcagttt ctggttcgtg agggaatcgt cgaactcaac cccgcgaagc
      901 tcgtcgccac gccgcggttg gaaaagaaac tgccggcgca tctctcaatc gaagaggcga
      961 tcaggtttat cgagtcaccg gatctcgaaa ctgatctggg caaacgtgat cgcgcgatgt
     1021 tggagttgat gtatgcgact ggcgtgcgcg tcgcagagct gaccacgctg aatgtttcag
     1081 acgtggatct tcacaaccgt ttgatacgcg tcaccggcaa gcgacgcaaa cagcgcattg
     1141 ttccatttgg cgatccggca ggtgatgcgc ttcgcggtta tcttgcagtg cgcggcaagt
     1201 ttctcttgaa cgcgcctgtt tcgaagcgcg acgaagaggt gttgttcctg aactaccagg
     1261 gaacacggat cacaactcgt tcggttggaa gaatggtgga gaagtacatc cgtctttgcg
     1321 cgggcatgca caacatcagc ccacacgccc tgcgacactc gtttgcgacc cacctgctcg
     1381 acagcggcgc tgacctgcgc gacatccagg aactgctggg ccatgcgcgt ctttcaacca
     1441 cacaaatcta cacgcacgtt tcaatggaga agttagtcga ggtctacgac aagtcacacc
     1501 cgaaagcgtg agccacagat ttacaccgat tgtttagagc gtcggtaggt cctcttcgcc
     1561 gttggcgctc tcgattattg cctggcaggc ggcgtcgtaa cgggcggtgg ggagcgcttc
     1621 gagacggcgt tcgtcgatgg gacggctgca ccgagcgcag atgccgtaac ttccttcctt
     1681 catccgcagt aaagcctgat cgatgtcggc gaccacttgt gagctgcgct cgcccaaccg
     1741 cagtgcgatc tctttactca agtcctgcac tgaaagatcc acgctgtctt tgacgtcatc
     1801 gtcatagaga tccagcgccg ctgcctgatc ggtccggatc tgttcggtgt actggcgtaa
     1861 ttgattgaga agaaggcttc ggaagtgttt cagtttttcg gcgtccatgg ttttggtggg
     1921 cgccagtata gcattagtcg ccaaccgcgt ccggcaatgg attcgtgaca tttgtgatta
     1981 tccggtgtgc gcgatgagca tcgcggcgag cagcgggatc gttaactcgt ggtggccggt
     2041 gatcgaatga ccttcgcccg cgcctttctc cgtggggcgc cgaacgacat tggtcagcgg
     2101 acggtaggat tgaatgaagt cgaagtttgc ggtggttatc ttcgaaagtg gatggccgag
     2161 attgcgcacg agggttacgc acttcagaaa aacctccggc agcaccactg ccgaaccgat
     2221 gttcagataa acgcctccac cgtccattcg tctcaccagc tcagccagta gacgaaagtc
     2281 ggtgtgcgtg gttgcgccga gggcggcgcc gtctgcactg ggatgaaagt gagcgatgtc
     2341 tccaccgatc gtcaggtgga ttgtaaacgg cactctcgtt ttgtaagcac cgcagagcaa
     2401 cgagtactgc tcgtgttgcg gctgcattgc taacagattt cttccgacgg cttctcccaa
     2461 cccgatccta tcggcagctc cgtcctgtgc agcgcgattg agtaattggc cagtttcatc
     2521 cgcgccgccg aagatgcctt ccgatagcgt cgcgtcaaca tcttccgaag ttgatccgat
     2581 catcgcgatc tccgcgtcat ggaccagcac ggatccgttc gcggcaattg ccgagacata
     2641 gccacgctcc atcaaatcaa gaatcaccgg cgccaatccg gtttttatga cgtgcccacc
     2701 gaggccccag atgatcggct tctccaactc tctcgcgcgt ctcatccgct cgacgatctt
     2761 cctgagcgcc tgcacggcga gcacgtcagg cagactcgcg ataaactcct ctaccgatgc
     2821 agcgtcactt agcggccgcg cgaaattttc cagactgact ttactggtac gtgaggcaag
     2881 cgggtagttt cttacgttct tgagctcgag gggttcggct tcataaatgg atggcaagat
     2941 gacctctgta aaatattacg gaaggttctc taaatccata atgtctttag atctgccact
     3001 cgcttttttg ttttctcgaa gtctcggcaa acttatcata ttgacttcaa cgccatcgag
     3061 ctcgccgtgg accctctcaa catagcactc agcgaagttg acgccatcga ttgttgtcat
     3121 tatttctata cgaaatggtg ctatacccat ccgggccaac tgattgtcct gttgcaatag
     3181 ttcggcgggg cagctaaaac cgaattcctg caaggcgccg agagcacgct cggcattctc
     3241 gcgagtgcgg gaaatccaaa tatccaagtc gccggtggga cgaggatagc cgtggtaacc
     3301 cactgcgtag ccgccgacaa ttagatactc aacgtttctt gagttgagta atctcaaaaa
     3361 ctctttgaag tctggcggta gctggatcgt acccataggc gctctgtcgc atcagctcaa
     3421 gggctagcag tctttcttct ggagtacgcg agtgccagta ctcacgatcg gcagcatcgg
     3481 cctcctcgaa cgatgagaaa atcttaacga ctgtcttatc aagtctcatg ttgtttagtg
     3541 acgcttccat aacgagcacg attataccac gtgttcagtt acggcctttg cggttatcga
     3601 tgagcattgc cgttgccatt acgtgtgacg cggccgttga ggaggggacg aagaacttgt
     3661 ccggtgtggg agcggcgcga gc
//
LOCUS       DASTEY010000348         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1491152, whole genome shotgun sequence.
ACCESSION   DASTEY010000348 DASTEY010000000
VERSION     DASTEY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491152"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFO72_06150"
     CDS             <1..1149
                     /locus_tag="VFO72_06150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9478905.1"
                     /translation="WLCCQLGAREHYAVPRALQTRGLLHELITDLWIRPGRLPTWRNK
                     LTGRFHPDLAGVRVSAPNMAALAFELKSQATGFHGWDLIAKRNEWFQDHVLVQLERRA
                     SELGDGLVTVFAYSYAADRIFEFARERGWRTVLGQIDPGPAEERRVADSTPAPESYWR
                     RWRSACDLADRILVNSEWSRDALLKEHLPVNKIHVIPLALEPGKDAALFNRVYPAAFD
                     AARPLRVLFLGQINHRKGAAQLLDAARLLAQAPVEFWFVGPRQIRTDLRSESKIKWLG
                     AVPRTAVGRFYREADIFILPTLSDGFGLTQLEAQSWKLPVLASRFCGAVVQHGRNGLI
                     LDQVAGESIAATLRELLESPERLAAMSQESRVSDEFTLKSLASSLTQL"
     gene            1149..>2207
                     /locus_tag="VFO72_06155"
     CDS             1149..>2207
                     /locus_tag="VFO72_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478906.1"
                     /translation="MQSLTWNRPLAIAATPGFRVRTTLLAILCTSMATALAILLIPES
                     PGNPGALFYPALAMSAGLAIAPLKAAFRYPKALLHGESLLTLAPIYWLLLDLLQGVYS
                     MDDITADQIRQAFLAIGLFVVMVWVGSVRQPWTTPALLTRSVSQEFSVRTYFALALAC
                     FFLGMLTFAIPSNFNLFEMAYYVGQDRWAAPWSRGQLGGWDAFLDHLQYFGYLLPILT
                     VVIGRRVGLRNARTLTCIVLSIVMTLFLAQSGSRRVIGVVAGMALVLWILDQQQLRVR
                     HLIETALAVAALLLVLQVMLEYRNVGLGVLVGKGDATVGRMEKRQILEEQHLRVDDNF
                     YRLCQIIQLMPQPYGFVYH"
BASE COUNT          383 a          614 c          681 g          529 t
ORIGIN      
        1 tggttgtgtt gccagctagg tgcgcgagaa cactatgcag tgccgcgcgc cttgcaaaca
       61 cgtggactct tgcatgagct gatcaccgat ctctggatcc gcccgggcag gctgccgacg
      121 tggcgcaaca agctcaccgg acgttttcat ccggacctcg ccggcgtgcg cgtgagcgcg
      181 ccgaatatgg cggcgctcgc gtttgagttg aagtcccaag cgacaggctt ccacggttgg
      241 gatttgatcg ccaaacgtaa cgagtggttt caggatcacg tgttggtcca actagagcgg
      301 cgcgcgagtg agttgggaga tggattagtg acggtctttg cgtacagtta cgctgcggat
      361 cggatctttg agtttgcgcg tgagcgtggc tggcgcaccg tcctcggcca gatcgatcca
      421 gggccggccg aagagcgacg agttgccgat tcgacaccag ctccggaaag ctactggcgc
      481 cgttggcgca gtgcgtgcga cctcgccgat cgaatccttg ttaattcgga gtggtcacgc
      541 gacgcgctgt tgaaagaaca cctaccggta aataagatcc atgtcattcc acttgctctg
      601 gagccaggca aagacgccgc actctttaac cgcgtctacc cggcagcctt cgacgctgct
      661 cgccccctga gagttttgtt tctgggccag attaatcacc gcaaaggggc cgcgcagttg
      721 ctggacgctg cgcgtttact ggcgcaagcg ccggtcgagt tttggttcgt tggaccaagg
      781 cagatccgca cggatttgcg aagcgaatcg aagatcaaat ggttgggtgc ggtgccgcgt
      841 acggcggtcg gccgttttta tcgcgaggct gacattttca ttctgcccac cctttcggat
      901 ggctttggcc tcacgcaatt agaagcgcag tcgtggaagt tgccggtgct ggcttctcgt
      961 ttctgcggcg cagtggttca acacggccgc aacggtctga tactcgatca agtcgcgggc
     1021 gagtcgattg ccgcaacgct tcgagaactg ctcgagtcgc cggaacgtct agctgcaatg
     1081 tcgcaggagt cgagagtcag cgacgagttc actttgaaat cgctggcctc gtctttgacg
     1141 cagttgtaat gcagtcactt acctggaacc gtccactcgc gatcgcggcc acgccgggct
     1201 ttagggtgcg aacgactctg ctcgcgattc tgtgcaccag catggcgacc gcattggcta
     1261 tacttctgat ccccgaatcg ccgggcaacc cgggtgcatt gttctacccg gcactcgcga
     1321 tgtctgccgg tctggcgatc gcgccgctca aggccgcttt ccgctatcca aaggcacttc
     1381 ttcacggcga atccttgctc actctcgcgc caatctactg gttgctgctc gacctgttgc
     1441 agggtgtgta ctcgatggac gacatcaccg cggatcaaat ccggcaggcg tttctcgcga
     1501 tcggattgtt tgtcgtgatg gtgtgggttg gctccgtgcg ccaaccatgg accacgccgg
     1561 ccctgttgac tcgttcggtt tcgcaggagt tttcggttcg cacgtatttc gctctcgcgc
     1621 tcgcgtgctt cttcctcggc atgttgacct ttgccattcc gtccaacttt aacctgttcg
     1681 agatggccta ctacgtgggt caggatcgtt gggccgcacc atggagccgc gggcagttag
     1741 gtggttggga cgcttttttg gaccacttgc aatactttgg ctacctgctg ccgattctga
     1801 cagtggtgat tggcaggcgt gtcggtttgc ggaacgcccg cactcttacc tgtatcgtgc
     1861 tgtcgatcgt gatgacgctg tttctggcgc agagcggcag ccggagagtc atcggcgtgg
     1921 tggcgggcat ggcgttggtg ttgtggatcc tggaccagca acagttgcgc gtcaggcatt
     1981 taatcgagac tgcgctagca gtggccgcgc tgttgctggt tttgcaggtt atgctcgagt
     2041 accgcaacgt cgggctcggg gtattggttg gcaagggcga cgcaactgtt ggccggatgg
     2101 agaagcggca gatacttgaa gagcagcact tgcgcgtcga cgacaatttc tatcgcttgt
     2161 gccagatcat tcaactgatg ccgcagccgt atgggtttgt ctatcac
//
LOCUS       DASTEY010000349         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_551454, whole genome shotgun sequence.
ACCESSION   DASTEY010000349 DASTEY010000000
VERSION     DASTEY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551454"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFO72_06160"
     CDS             complement(<1..1062)
                     /locus_tag="VFO72_06160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9478907.1"
                     /translation="MLSKVIFVALLLVVAITPVPYGTVEPWWKAAFICAVLGIFIFGI
                     FENGNFFNPWRTLLPLVALSAFAFLQSWTRISTDPFQTRFFALQLLALTAFLALLYRY
                     AASEHRVRILIYVIFAVAITSAIFGILRQTTQQQTGFLLPLLKPNQGFAQFINKNHFA
                     YLMEMALGLGLGLIIAERSRQRLFVMSLLLPIWVALVLANSRGGIVVMLAQLLIAALF
                     LMKWNTLRIATAAVLLAGVLVGTLWVGGDRLVINFSTAGTELTANSAVASDGASRNEI
                     WRATLKMFAAHPLLGAGLGGYWIGITRFHAASGVLTPQEAHNDYLELLSSGGLVGLAL
                     GVWFAVNVVRLTRQRLWSDT"
     gene            complement(1066..>2163)
                     /locus_tag="VFO72_06165"
     CDS             complement(1066..>2163)
                     /locus_tag="VFO72_06165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET9478908.1"
                     /translation="GLDGLASGAALFASLVILGVSIAQERSLMIVVSLVLCGAVAGFL
                     RYNFNPASIFLGDSGALFIGFLLAAMSVLGTQKATTAVAIFVPILAFGFPVVDTAMTM
                     GRRIISRKPVFEGDNEHIHHMLLARGWSQRRVALVLYGVCALFGLLALIFPASGPKLT
                     GVMLFVISVAVIIAVGHLRYHEVDEIRAGVKRTVGDRRLRVANNIRVRRTARALAKAS
                     DLHELFEAVRQMLDVGEFTFANAQIGQAGRAEINERAFEASLQRHPKQALEFRNGRVF
                     WSWSNDGGDRNEFAAREEWSFRMPLVKDGMEWGWVNFYHSLDGETLLVDTNYLSDLFR
                     REFTDAAARVFRMHELPEIAPAMAMHATAKG"
BASE COUNT          529 a          649 c          590 g          395 t
ORIGIN      
        1 ggtgtcggac cacagccgct gtcgcgttag tctaacgaca ttcaccgcga accaaactcc
       61 aagcgccagc ccaacgaggc ctccgctcga cagtaactca agataatcgt tgtgggcttc
      121 ctgcggtgtg aggacgccgg acgcagcgtg aaacctggtg atgccgatcc agtaaccacc
      181 gagtccggcg ccaagcagcg gatgggctgc aaacatcttc aaggtcgcgc gccagatctc
      241 gtttcgactg gcgccgtcgc tcgcaactgc ggagttagca gtcagctcgg ttccagctgt
      301 gctgaagttt attacgagtc gatcgccgcc gacccacagt gttcctacca acactcccgc
      361 gagtaaaacc gcggctgtgg ctattcgcaa cgtgttccat ttcatcaaga acaaggcggc
      421 gataagtaac tgggcaagca tgacaacgat tccgccgcgt gagtttgcga ggacgagcgc
      481 cacccagatt ggtaacagca acgacataac gaacagtctt tggcgcgagc gttcggcaat
      541 gatcaggccc agtcccaggc ccagcgccat ctccatgagg tatgcgaagt ggtttttatt
      601 gatgaactgt gcgaagccct ggtttggctt caatagtggg agcagaaaac cggtttgctg
      661 ttgggtcgtt tggcggagta tgccgaagat cgcgctcgtg atcgcgacag caaagatcac
      721 gtagatcagg atgcgaacac gatgctcgct ggcggcgtag cgatagagca gtgccagaaa
      781 cgcagttaga gcgagtaact gtaaagcaaa gaagcgtgtt tggaatggat cagtggaaat
      841 ccgtgtccac gactggagaa acgcaaaggc tgacagggcc actagcggga gtaacgttcg
      901 ccacggatta aaaaaattcc cgttttcaaa tattccaaaa atgaagatcc ccaaaaccgc
      961 gcagatgaaa gccgccttcc accagggctc gaccgtgccg taaggaaccg gtgttatcgc
     1021 caccaccaac agcaacgcta cgaagataac cttgctcagc aaaacctaac ccttcgccgt
     1081 cgcgtgcatc gccatcgccg gcgcgatttc cggcagttcg tgcatgcgga agacgcgcgc
     1141 cgccgcgtcg gtgaactcgc gccgaaagag atcgctcagg tagtttgtgt caaccaacaa
     1201 tgtctccccg tccagactgt gatagaaatt cacccagccc cactccattc catccttaac
     1261 gagtggcata cgaaaagacc actcctcacg cgccgcgaac tcatttctgt cgccgccatc
     1321 attggaccac gaccagaaca cgcgcccgtt ccgaaactcc agcgcctgtt tcgggtggcg
     1381 ttgcaaactc gcttcgaaag cacgttcgtt gatctccgcc cggcccgcct gaccgatctg
     1441 cgcgtttgca aacgtgaact cgccgacgtc aagcatctgg cgcaccgctt caaacagctc
     1501 gtgcagatct gacgctttcg ccagtgccct tgcggtgcgg cgtacgcgaa tgttgtttgc
     1561 aactcgcaga cgccgatcgc ctacggtgcg tttcacgccc gcccgtatct catccacttc
     1621 gtgataacgc aagtggccca cggcgatgat tacagcaacc gagatcacaa acagcatgac
     1681 gcccgtcagc ttcggtccac tcgccgggaa gatcagcgct aataacccaa acagagcgca
     1741 aacgccgtag agcacgagcg ccacgcggcg ttgcgaccaa ccacgcgcga gcagcatgtg
     1801 atgaatgtgt tcgttgtcgc cttcaaagac cggtttacgg ctgatgatcc ggcggcccat
     1861 cgtcatcgcc gtatcgacca ccggaaaacc gaaagcgaga atcgggacga agatcgccac
     1921 cgcagtcgtc gccttctggg ttcccaaaac cgacatcgcc gccagcagaa atccgatgaa
     1981 tagggcacct gagtcgccga gaaagattga tgcgggattg aagttgtaac gaaggaaacc
     2041 ggcgactgcg ccgcagagga cgagcgagac cacgatcatc agcgagcgtt cctgcgcgat
     2101 tgaaacaccg agaatgacca gcgaagcaaa gagcgctgcg ccggacgcga gcccatcgag
     2161 acc
//
LOCUS       DASTEY010000350         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_403333, whole genome shotgun sequence.
ACCESSION   DASTEY010000350 DASTEY010000000
VERSION     DASTEY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403333"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFO72_06170"
     CDS             <1..711
                     /locus_tag="VFO72_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478909.1"
                     /translation="GLQNSTSFGTAFIVNALTPEANYSSSDFDARHIINANWVVGLPF
                     GRGKRFFSNASGFVEQAFGGWSTTGIFRWNSGLPVTGAPFDCCVWATNWNVQSNGVRV
                     QPIQSSTVKTGENGTPNLFSDPLAAYRSFRPARPGEVGDRNVLRGQGYISLDMGLYKS
                     FKMPWEGHALQFRWEVFNVTNTQRFDTLTISDLSLGADPFLGGNPASDFGQYTSTQTP
                     LNETKAGRVMQFALRYTF"
     gene            788..>2288
                     /gene="recN"
                     /locus_tag="VFO72_06175"
     CDS             788..>2288
                     /gene="recN"
                     /locus_tag="VFO72_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET9478910.1"
                     /translation="MLQFLNISNIALIDDMQVEFESGLNLLTGETGSGKSIIVDALGV
                     LIGGRFTSELLKTGAERAAIEGLFSVTANPDLEVLLENAGLGATNELIIRRELSATGR
                     NKIFINNQLATQSLLRDLRPFLVDIHGQGEQQTLFNPETHLEILDAFAGNTALRVEVA
                     EAYQRWIAVRRELDSLRHDEAEKFQLVDTLKFQIAELERAHLSIGEDELLEEERRRLA
                     NVEKLTELCQSSYSRIYEDDDAAVARVRQTLKDVEELAEYDSSFRDYLEGLESARAVL
                     EDLSFTLRDFADRLEFSPSRLAEIEDRLAELSRLKRKYGGSIAASLEHLARSEDRLRQ
                     IETSDERERELNAELAAARERYLELAGKLHKERVRAAKKFEQAVEKGIGEVAIDNARF
                     QVQVSDNVELAGQKGIDHTEFYFSANVGEEVKPLARVASGGEASRLMLVLKTVANGSQ
                     FPRTIVFDEIDTGIGGRVSEAVGIKLKKLAQTNQVLCVTHQPQIARFADS"
BASE COUNT          504 a          592 c          699 g          493 t
ORIGIN      
        1 ggtctgcaaa actcgacgag ctttggcact gcgttcatcg tcaacgcact gactccggaa
       61 gcgaactact ccagctcgga tttcgacgcc cggcacatca tcaatgccaa ctgggtggtc
      121 ggacttccgt tcggacgcgg caagcggttc ttctcgaacg cgagtggttt tgtcgagcag
      181 gccttcggcg gctggtctac caccggaatc ttccgttgga acagcgggct tccggtaact
      241 ggcgcgccgt tcgattgttg cgtctgggcg acgaactgga acgttcaatc gaatggtgtc
      301 cgagttcaac cgatccagtc gtcaacagta aagaccggcg agaacggaac gccgaacctc
      361 ttcagcgatc cgctcgctgc gtatcgcagt ttccgtccgg cgcgtccagg cgaagtgggc
      421 gacaggaacg tgcttcgtgg tcaggggtac atttccctcg acatgggcct gtacaagtcg
      481 ttcaagatgc cgtgggaggg ccacgcactg cagttccgtt gggaggtctt taacgtcacc
      541 aacactcagc ggtttgacac tcttacaatt tcagacctga gtttgggggc ggatccgttc
      601 ctcggcggga acccggcttc cgacttcgga caatacacct caactcaaac gcctttgaat
      661 gaaaccaaag ccggacgcgt aatgcagttc gcgctgcggt acacgttcta aggaaaaaac
      721 atcggtcacg gatttcgcgg attacacgga ttgcataatc tgtgtaatct gcgaaaatca
      781 gtggccgatg cttcagttcc tgaacatctc caacatagct ctaatcgacg acatgcaggt
      841 tgagtttgag agtggtctca atctgctcac cggtgagact ggatccggga agtcgatcat
      901 tgtcgatgcg cttggtgtgt tgattggcgg caggttcact agcgagctac tcaaaacagg
      961 agctgagcgg gcggcgatcg aagggctctt ctctgtcact gctaatccgg atctcgaagt
     1021 cctgctcgaa aacgcggggc tcggcgccac caacgagttg atcattcgtc gcgagctctc
     1081 cgctaccggg cgcaacaaga tctttatcaa caatcaactt gccacacagt cgctgctgcg
     1141 tgatctgcgg ccgtttctgg tcgacattca tgggcaggga gaacagcaga cgctcttcaa
     1201 tccggagact cacctggaaa tactcgacgc gttcgcgggt aacactgcgc tgcgggttga
     1261 agtcgccgag gcgtaccagc gctggattgc agtgcgacgc gagctcgatt cgttgcggca
     1321 tgacgaagcg gaaaagtttc aactggtcga cacgttgaag tttcagatcg cggagctcga
     1381 gcgtgcgcat ttgtcaattg gcgaagacga actactggaa gaagaacgac gccggttagc
     1441 gaacgtggag aagctgacgg agctttgcca gtcgagttac agccgcatct atgaggatga
     1501 tgatgcggcg gttgcgcgcg tgcggcagac gttgaaagat gtcgaggagc tggctgaata
     1561 cgattcgagt tttcgcgatt atctcgaagg actggaatca gcgcgcgcag tgctcgaaga
     1621 tctttctttc acgctgcgtg atttcgcgga tcgattggag ttttctccgt cgcggctggc
     1681 tgaaatcgag gacaggctcg ccgagctttc gcggctaaaa cgcaagtatg gtggctcgat
     1741 cgccgcgtca ctggagcacc ttgcgcgctc tgaagatcgt ttgcgacaga tcgagacatc
     1801 cgatgaacgt gaacgggaat tgaatgccga gttggctgcg gcgcgtgaga gatatctgga
     1861 gctggcgggc aagctgcaca aggagcgggt gcgtgcggcg aagaaatttg agcaggccgt
     1921 ggaaaaagga atcggtgaag tggcgatcga caatgcgcgc tttcaggttc aggtctctga
     1981 caacgtcgaa ttagcgggac agaaaggcat cgatcacact gagttttact tctcggcaaa
     2041 cgtcggcgag gaggttaagc cgcttgcgcg ggtggcctcg ggtggcgagg catcgcgtct
     2101 gatgctggtg ctcaagactg tcgcaaacgg atcgcagttt ccgcggacga tcgtattcga
     2161 tgagatcgac accggcatcg gcgggcgcgt tagtgaggca gtaggcatca agctcaaaaa
     2221 gctggcgcaa acaaatcagg tattgtgcgt tacgcaccag ccgcagatcg cgcgcttcgc
     2281 cgatagcc
//
LOCUS       DASTEY010000351         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1375702, whole genome shotgun sequence.
ACCESSION   DASTEY010000351 DASTEY010000000
VERSION     DASTEY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375702"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFO72_06180"
     CDS             <1..765
                     /locus_tag="VFO72_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478911.1"
                     /translation="APLPKRTAFLLGFVAPAIVLGGWCLLTYGGLVQPDFLPKPTEVI
                     RGTLQLFIQYDLGTAIIVSTRRIAVAFLLASVIALPLGVLMGAFEPINRFFEPIMAPL
                     RYMPISAFIPLLILWFGIYEKQKIAFLFLGVFVYLLPVVVSSIRLVPEELVQTALTLG
                     ASKLQVVTTVLVPAALPEIFDSFRVMNAIAWTYVILAEAVNPEYGLGYMVELARTHQK
                     ASWSFAGLLVIGGIGLLTDFLIRLLSSLLFRWRETT"
     gene            771..1667
                     /locus_tag="VFO72_06185"
     CDS             771..1667
                     /locus_tag="VFO72_06185"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478912.1"
                     /translation="MADNDAQATLPQSGEPGTIAVDIGEPKPKPTYRADQSGAPPKLR
                     VSNLSVTYIDRKGNRTEAVRDVSFDILDKPNSGEIVVFLGPSGCGKSTILKAVAGLLP
                     PTEGEVLVDGELVTDVGRDRGMVFQAYTSFGWLTVRQNVEYGLKLRGTPGSERRKISD
                     AILQEVGLADFADRYPKDLSGGMKQRVAIARTLVNKPRVVLMDEPFGALDPQTRWGMQ
                     SLLLEISRIENNTILFVTHDVSEAVYLGDTIYVLSQRPARILHQVNVPAFPVRDIALK
                     SATEFRAVEKQLLDLIYAPVRS"
     gene            1783..>2494
                     /locus_tag="VFO72_06190"
     CDS             1783..>2494
                     /locus_tag="VFO72_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478913.1"
                     /translation="MGTTGKQGFTKPSQTAVRKDGVPVNITVLRDDPIAATQAFVRQK
                     VHEKRSYVKAAFANPYNLSLLAGGLVASALTLNPLLAIVTLGLEVLWVVNAPGSKKLR
                     EWLWDPQFEAEEQKQLQELRAKQLHNLDEVDRERVVALIARQQQIDTLASQNPSFTGE
                     LLRSELTKTDRLVEAFIEMATTCSRYEAYLDSINLSELERDRRRWEAIVKDQNIHNSQ
                     SDIAAKNLAVIMKRFEKMK"
BASE COUNT          520 a          713 c          702 g          559 t
ORIGIN      
        1 gcgccgctgc ctaagcgcac ggcgtttctg ctcggcttcg tcgcacccgc gattgtcctg
       61 ggcgggtggt gcctgctgac ctacggcggc ctggtccaac cagactttct gccgaaacca
      121 accgaagtca tccgcggcac gttgcaactg ttcattcaat acgatctcgg cacggcgatc
      181 atcgtctcca cgcgccgcat cgcggtcgcg ttcctgctcg cttcagtgat cgctttgccg
      241 ctcggcgtgc tgatgggtgc tttcgaaccg atcaatcgtt tcttcgaacc catcatggcg
      301 ccgctgcgtt acatgccgat ctccgcgttt attccgttgc tgattctctg gttcggtatt
      361 tacgagaagc agaagatcgc gtttcttttt ctcggcgtgt ttgtttacct gctgccggta
      421 gttgtctcgt cgattcgact cgtgccggaa gagttggtcc aaactgcgct aacactcggc
      481 gcgtcaaagc ttcaggtggt tacaacggta ctcgtgccgg ccgcgctgcc ggagatcttc
      541 gacagcttcc gcgtgatgaa cgcgatcgcc tggacctacg tcattcttgc cgaagccgtc
      601 aatcccgaat acgggctcgg ctacatggtc gaactcgcac gcacgcacca aaaagcgtcg
      661 tggtcctttg ccggactgct ggtcatcggc ggtatcggtc tgcttactga ttttctcatt
      721 cgccttcttt caagcctgct gttccgctgg cgtgaaacta cctgaacctt atggccgaca
      781 acgacgctca agccacactt ccacaatcag gcgaacccgg cacgattgcc gttgatatcg
      841 gcgagccgaa gccgaagccg acgtatcgcg cggatcagag cggcgcgccg ccaaagttgc
      901 gcgtcagtaa tctgagcgtc acgtatatcg atcgcaaagg caatcgcacc gaagcagtgc
      961 gcgatgtttc cttcgatatt ttggacaagc ctaactccgg cgagatcgta gtttttcttg
     1021 gaccatcggg ttgcggcaag tcgacgatcc tgaaagcagt cgctggtctg ttaccaccga
     1081 cagaaggtga agtgttggtt gacggtgaac tagtcacgga cgtcgggcgc gatcgtggca
     1141 tggtgtttca ggcttacacg tctttcggct ggctcaccgt acgccaaaac gtcgagtacg
     1201 gtctgaaact caggggaaca cccggatcgg aacggcgcaa gatctcggac gcgattctgc
     1261 aagaggttgg gctggctgac ttcgccgatc gttatccgaa agatctttcc ggcggcatga
     1321 agcaacgcgt ggccatcgcg cgcacgctcg tcaacaagcc gcgagtggtc ctgatggacg
     1381 aaccgttcgg cgcgctcgat ccgcagacgc gctggggtat gcagagtctg ctgctcgaga
     1441 tctcgcgtat cgagaacaat acgattcttt ttgtcactca cgacgtttcc gaagcggtct
     1501 acctgggcga cacgatctac gtgctgtcgc aacgtccggc gcgaattctt catcaagtca
     1561 acgtccccgc gtttcctgtc cgcgacattg cgctaaaatc agcaacggaa ttccgggccg
     1621 tggaaaagca gttgcttgac cttatctacg cgcccgttag gtcgtaatgg tctcgcgcaa
     1681 aggcgcaaag gcgcaaagct gactcgtcag tacaagattc tttgcgtctt tgcgcctttg
     1741 cgggaaactt tcctggtact tgcttagcga ctgaagattc gaatgggaac aaccggaaag
     1801 cagggcttta cgaagccgtc acaaacggcc gtgcgcaagg acggtgtgcc ggtaaatatc
     1861 acggtgctgc gcgacgatcc catcgcggcg acccaggctt tcgtcagaca gaaagtccac
     1921 gagaagcgtt cttatgtcaa agctgcattt gcgaatccgt acaacctctc gctgcttgcc
     1981 ggcggactcg tcgcttcggc gctgacgctc aatccgttgc tggcaattgt cacactgggc
     2041 ctcgaagtac tctgggtggt gaatgcgccg ggctcgaaga agcttcgcga gtggctttgg
     2101 gatccacaat tcgaagcgga agagcagaaa cagttacagg agttgcgcgc gaagcaactt
     2161 cataatctag acgaagtgga tcgcgagcgc gtcgtggccc taattgcgcg gcaacaacag
     2221 atcgatacgc tggcctcgca gaacccttct ttcaccggcg agttgctgcg tagcgaattg
     2281 actaagaccg accggctcgt ggaagcgttt atcgagatgg ccacgacgtg tagtcgttac
     2341 gaggcttacc tcgactcgat caacctgagc gaactcgaac gcgaccggcg ccgttgggaa
     2401 gcaatcgtca aagaccagaa catacataat tcgcaaagcg acattgccgc gaagaacctc
     2461 gccgtgatca tgaaacgttt cgagaagatg aagg
//
LOCUS       DASTEY010000352         5139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_881387, whole genome shotgun sequence.
ACCESSION   DASTEY010000352 DASTEY010000000
VERSION     DASTEY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881387"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VFO72_06195"
     CDS             complement(<1..1373)
                     /locus_tag="VFO72_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478914.1"
                     /translation="MTTLFSPRTLVAIAAAFVLALVLTPLVRAFARRVGMVAVPKIDR
                     WHKKPTAMMGGIAIWLTVIICYFVFIPRTVYLAEILLASTFLFLVGLVDDVIHIKPYQ
                     KLIGQVLGSAFVVYYGLSLPWTGSVLLNMALAIFWLIGITNAINLLDNMDGLASGIAI
                     IAAGFLALSFINTGQFTEALIMLLFAGALLGFLVYNSNPASIFMGDCGSMFVGFFLAS
                     SALINVSGGRSRSFLPVLAVPILVLFIPIFDTTFVTVLRKLSGRAASQGGRDHTSHRL
                     VALGMSERHAVWMLYGLAALSGVLAILVQRARLDVSLAAIAGFTILLTLLGVYLGGVK
                     VYDQSDEETALKEKPLYAFLVDVSYKRRIFEVLLDVVLIILSYWAAYAINFPPNSPAW
                     QLFIRTLPVLVFVKMSVFLVMGVYRGLWRYTSIGDLIVFGKAVVISSVASLLVLLFAF
                     RFEGFSR"
     gene            complement(1467..2435)
                     /locus_tag="VFO72_06200"
     CDS             complement(1467..2435)
                     /locus_tag="VFO72_06200"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9478915.1"
                     /translation="MRVLITGGAGFIGSHLADAYIQRGDEVYVIDDLSTGTIENIQHL
                     KGKPRFHYTIETVHNQPVTAELVDQCDVIFHLAAAVGVKLIVESPVRTIETNVRGTEV
                     VLSLANKKKKKVLIASTSEVYGLSSDVPFREDGNLVMGATTKGRWSYACSKAIDEFLA
                     LAYWREKKLPTIVVRLFNTVGPRQTGQYGMVIPTFVKQALAGRPITVYGDGKQSRCFG
                     HVGDVVGALIKLMENDNAVGNVFNIGSNQEITILELAQRVKHLTQSDSEIVFVPYDEA
                     YEEGFEDMPRRVPDISKVSELVGFRPQMSLDGILESVIDYQSGRRK"
     gene            complement(2438..3715)
                     /locus_tag="VFO72_06205"
     CDS             complement(2438..3715)
                     /locus_tag="VFO72_06205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478916.1"
                     /translation="MPDAQHAKVVRIIARLNVGGPAKHVVWLTSGLEAAGYSSLLVAG
                     SVPEGEEDMSYFAEQTGVKPLYIPEMSREISLKDVITAWKLFRLLLRERPDIVHTHTA
                     KAGTVGRVAGFCYRWLTPGIVIGRPRPCKFVHTYHGHVFHSYYGPLRTRAFLTIERLL
                     AKLATDRIVVVSEQQKAEINETFRVGRRDQMRVVRLGLDLGIFANHESRRAKFRKELG
                     VDDATILVGIVGRLTEIKNHRMFLQSVARVNSANVRYVIIGDGSLREPLEREARSLGL
                     DKILIFAGGRKDPEYFYPALDICALTSLNEGTPLTLIEAMANARPVIATSVGGVADLV
                     GNVVEKGSYDVCERGISVPPSDVDAFAAGLRRLIEDKALRDQLGRRGFEFVDRNYRKE
                     RLLEDIKDLYGQLLNRERTANCELPVAKYSQGA"
     gene            complement(3928..4698)
                     /locus_tag="VFO72_06210"
     CDS             complement(3928..4698)
                     /locus_tag="VFO72_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9478917.1"
                     /translation="MKGVVLAGGLGTRLRPLTSVTNKHLLPVYNQPMIFYPIQTLVNA
                     GISDIMVVTGGSSAGDFLRLLGNGKAFGLKHLNYAYQEGEGGIAEALSLVEHFAANEP
                     VCVVLGDNIIQGNICAAVEDYRKQGRGAKIMLKKVPDPQRFGVPKLNGRRVVYVEEKP
                     AVPPSEYAVIGVYMYDSDVYDIIRSLKPSKRNELEITDVNNAYIARDEMTWDELEGWW
                     TDAGTFESLLRASNLVAETGANRLDLVPEAVAVNAQQV"
     gene            complement(4718..>5139)
                     /locus_tag="VFO72_06215"
     CDS             complement(4718..>5139)
                     /locus_tag="VFO72_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9478918.1"
                     /translation="ALLEEVARILRPGGLFWTTTPHARGLSARVLGLKWRCIWPPEHL
                     QLFSIRGLKSLLRDAGFRDIRVDTTGGNPIEIWHALGRTKSAPQTVDQHFDRVLTSYQ
                     LNESLMKSRSRRALKSTVNSFLNLSRLGDSMKVFAIR"
BASE COUNT         1205 a         1544 c         1328 g         1062 t
ORIGIN      
        1 ttgcgcgaga agccttcaaa acgaaaggca aacaacagca ccagcaaact cgcgaccgaa
       61 ctgatcacaa ccgccttacc aaacacaatc agatcgccga tgctcgtgta gcgccacagt
      121 ccgcgataga cgcccatcac gagaaacacc gacattttga cgaacaccag cacgggcagg
      181 gtgcgaatga acagttgcca cgccgggctg ttgggtggga agttgatcgc gtaagcagcc
      241 caatacgaca aaatgatcag cactacatca agcagcactt cgaagatacg ccgcttgtaa
      301 gagacgtcga ccagaaacgc gtagagcggc ttctctttca gcgccgtctc ttcgtcgctc
      361 tggtcgtaga ccttcacccc accgaggtag acgccaagta acgtcagcag gatcgtaaat
      421 ccggcaatcg ccgcgagact cacgtcgaga cgcgcgcgtt ggaccaggat cgccagcacg
      481 cctgacagcg ccgcaagtcc gtagagcatc cagaccgcgt gacgttccga catacccaat
      541 gccacgagac gatgcgacgt gtgatcgcgc ccgccttgtg aagccgcgcg acccgacagt
      601 ttgcggagca ccgtgacaaa cgtcgtatcg aagatcggaa tgaaaagaac gagaatcgga
      661 accgccagca cgggcaaaaa acttcgcgag cggccgccgc tgacatttat cagcgccgaa
      721 cttgccagga agaacccgac aaacatcgag ccgcaatcgc ccatgaagat cgaggccgga
      781 ttcgagttgt agacaagaaa gccgagcagg gcgccggcaa agagcagcat tatcagcgct
      841 tcggtgaact gtccagtgtt gatgaaactg agcgccagaa atccggcggc gatgatcgca
      901 ataccggagg cgaggccgtc catgttgtcg agcagattga tggcgtttgt gattccgatc
      961 agccagaaga tggcaagcgc catgttcaag agcaccgatc cggtccaggg cagcgataga
     1021 ccgtaataga cgacgaaggc agagccgagc acctggccga tcagtttctg gtacggtttg
     1081 atatgaatga cgtcgtcaac gagcccgacc agaaacagaa acgtgctggc cagcaggatt
     1141 tcagcgaggt aaaccgtacg cgggataaag acgaagtagc agatgataac ggtcagccag
     1201 atcgcgatgc cgcccatcat tgcggtcggc tttttgtgcc agcgatcgat cttcggcacg
     1261 gcgaccatgc ccacacggcg cgcaaaggca cgcacaagag gcgtgagtac taaagctaaa
     1321 acgaacgcag cggcaatcgc caccagcgta cgcgggctaa aaagcgtggt cattgtttgc
     1381 attgacgatt ttcgattttc gattgccgat ttgcgccaaa ctcgaatggt tcttcaaatc
     1441 ggcaatcggc aatcgaaaat cgtcaatcac ttccgtcttc cgctctgata gtcgataacg
     1501 ctctcaagaa taccatcgag actcatttgt ggccgaaacc caaccagctc gctgaccttc
     1561 gaaatatccg gcacgcgccg tggcatatct tcaaaacctt cctcatacgc ctcgtcgtaa
     1621 ggcacaaaca cgatctccga atcagactgc gtcaggtgct tcactcgctg cgccaactcg
     1681 agaatcgtaa tctcctggtt cgacccgatg ttgaacacat tgccgacggc gttatcgttc
     1741 tccatcaact tgatcagcgc gccgacaacg tcgccaacgt ggccgaaaca acgactctgc
     1801 ttcccgtcgc cgtagaccgt aatcggacgt ccggcaagcg cctgcttcac aaaagtcgga
     1861 atcaccatgc cgtactgtcc ggtctgtcgt ggcccaacgg tattgaacaa ccgcacgacg
     1921 atcgtcggca gtttcttctc gcgccaataa gcaagtgcca aaaattcgtc aattgctttc
     1981 gagcaggcgt aactccaacg tcccttggtc gtcgcgccca tcaccaggtt tccgtcttcg
     2041 cgaaacggca cgtcgctact caagccatac acttccgacg tcgacgcaat caacaccttc
     2101 ttcttctttt tgtttgccag cgacagaacg acttccgtgc cgcggacatt cgtttcaatc
     2161 gttcgcaccg ggctttcgac aatcaacttc accccaactg cggccgccag atgaaatatc
     2221 acgtcgcact ggtctaccag ttccgccgtg acgggttgat tgtgaacggt ttcgatcgtg
     2281 tagtgaaaac gcggcttgcc tttgaggtgt tgaatgtttt cgatcgtccc ggtggaaagg
     2341 tcgtcgatga cgtagacttc atcgccgcgc tgaatataag cgtcagcaag gtgagatccg
     2401 atgaaaccgg cgccgcctgt gatgagtact cgcaccgtta tgctccttgt gaatatttcg
     2461 caaccggcag ttcgcaattc gccgttcgtt cgcgattcag caactggccg tagagatcct
     2521 tgatatcttc cagcaggcgt tccttgcggt agttgcgatc cacaaactca aacccgcgcc
     2581 ggcccaactg atcgcgcaaa gctttgtctt caattaatct cctcaaaccg gctgcgaaag
     2641 catctacgtc agaaggagga acactgattc ctcgctcaca cacgtcgtaa gaaccctttt
     2701 ccacaacgtt tcccaccagg tcggccacac caccaacgct tgtcgcgatg accggacgcg
     2761 cgttagccat cgcttcgatc agcgtcaacg gcgttccctc attcagcgaa gtcaaggcgc
     2821 agatatcgag cgccggataa aagtattcgg gatcttttct accacccgcg aagattagaa
     2881 tcttgtccag acctaacgat cgggcttcgc gttcaagtgg ttcgcgcaat gaaccatcgc
     2941 cgatgatcac gtaacggacg ttcgctgaat tcacgcgcgc cacactctgc aaaaacatcc
     3001 ggtggttctt gatctcagtc agccggccca caatccccac cagaatggtc gcgtcgtcaa
     3061 cgccaagctc tttgcgaaac ttcgcgcgcc ggctttcgtg attcgcaaag atccccaggt
     3121 ccaaacccaa gcggaccacc cgcatctgat cccttctgcc tacgcgaaac gtttcattga
     3181 tctccgcttt ctgttgctcg ctgacaacta cgatcctgtc cgtggccaac ttcgccaaca
     3241 gcctttcgat cgtcaaaaaa gcacgcgtgc gcagaggacc atagtaactg tgaaaaacgt
     3301 gcccatgata cgtgtgcaca aatttacatg gccttggtct gccgatcacg attccaggcg
     3361 tcagccagcg ataacaaaag cccgccacac gtccgaccgt tcccgctttc gcagtgtgag
     3421 tgtgaacgat gtcaggccgt tcgcgcagca ataggcgaaa cagtttccac gctgtgatca
     3481 cgtctttcaa agagatctcg cggctcatct cgggaatgta cagcggcttc acgcccgtct
     3541 gttcggcgaa atagctcatg tcctcttcgc cttcaggcac ggaacccgcg acgagcaacg
     3601 agctataacc cgccgcttcc agacccgaag tcagccagac gacgtgcttc gccggcccac
     3661 cgacgttcag tcgggcaatg atcctgacta cctttgcgtg ctgtgcgtca ggcatgagta
     3721 atcagcgaac gttctttccc aggcgtgtta accgtggacg cgtaatcccg gaccctagac
     3781 gcgttaaatc ctggctggcc ctggacgtgt taaccctggg accgcaggcg tctcgcctgc
     3841 cgtgtttcgc aggtagcccg aggcgacgat ttgcaggcga gacgcctgcg gtcccaggaa
     3901 aatcgcgtcc aaaaggtgac tcacgcatta aacttgttgt gcattcacgg cgaccgcttc
     3961 cggtacgagg tcgagtctat tcgcgccggt ttcggcaacg agattcgacg cacgcaacag
     4021 actctcaaaa gtgccggcgt cggtccacca accttccagc tcgtcccaag tcatctcgtc
     4081 gcgcgcgatg taggcattgt taacatcagt gatctccagc tcgttgcgct ttgaaggctt
     4141 cagcgaacga atgatgtcgt aaacgtcgct gtcgtacatg taaacaccga tcaccgcata
     4201 ctcggacggc ggtaccgcgg gcttctcttc aacgtagacc actcggcgcc cattgagttt
     4261 cggtacgccg aagcgctgcg ggtcaggcac ctttttcaac atgatcttcg cgccgcgacc
     4321 ctgctttcga taatcttcaa cggccgcaca gatgttgccc tggatgatgt tgtcgccaag
     4381 cacgacacac acaggttcat tggcggcaaa gtgttcgacg agcgagagtg cttcggcaat
     4441 tccaccctcg ccttcctggt aggcgtagtt gagatgtttc aagccaaacg ccttgccatt
     4501 gccgagcagt ctcagaaaat cgcctgccga acttcctcca gttaccacca tgatgtcgga
     4561 gataccggcg ttgacgagtg tttgaatcgg gtaaaagatc atcggctggt tgtagaccgg
     4621 cagcagatgt ttgtttgtga ccgaggtgag cggacgcaat ctcgtcccca agcctccggc
     4681 aagcacgacg cctttcaacg ttgtcctcca gatcgggtta tcgaatagcg aaaactttca
     4741 ttgaatcacc gaggcgactg aggttcagaa aactgtttac ggtgctcttg agcgcccggc
     4801 gcgaccggct cttcatcagc gattcgttca actggtaact ggtcaacacc cggtcgaaat
     4861 gttggtccac tgtctgcggc gccgacttcg ttcggcccag cgcgtgccag atttcgatgg
     4921 ggtttccgcc cgtcgtatcg acacgtatgt cgcgaaagcc ggcatctcgc agcagtgact
     4981 tcaaaccgcg aatcgagaat agctggagat gctctggcgg ccaaatgcag cgccacttca
     5041 aacccagcac gcgggcagac aggccgcggg cgtgcggcgt ggtggtccag aagagaccgc
     5101 cgggccgcag tattcgcgcg acctcctcga gcagcgctc
//
LOCUS       DASTEY010000353         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_255064, whole genome shotgun sequence.
ACCESSION   DASTEY010000353 DASTEY010000000
VERSION     DASTEY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255064"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VFO72_06220"
     CDS             complement(<1..1900)
                     /locus_tag="VFO72_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET9478919.1"
                     /translation="MRSAVISTKGHLMNATGGGTTLLALDTSRVRVDARLLSPLPANN
                     DAMFRVIVAGAATHDEAEASAKEIEKLTGEDTQETFDTETKTWGVAVGEKRSREEAEE
                     LRARLEAAGLDADVQAPPIYTDKTETLRATQSSQSTGSVKLTSRASSPSREVVASSTA
                     GRMFSSSAPVVFASDNEKDAPVRFNDRPYRGRIEVFTNLRGTLTVVNELGLEDYVRGV
                     VANELSPGGYPAIEALKAQAIAARTYALKNRGQFMAQGFDILPTTRSQVYRGLTSENP
                     LSTRAVDETRGLVATFAGEPINALYTSTCGGRTEDSENIFNHALPYLRGRECAAEGNA
                     AFERFVIRTTRTPAELRDDEHVALVRDVSLLLTQNFGTLQERVSDAWLAADASASEVR
                     NWIANVARVARQKMPVTGDDVNRPPPFATALLAAVFGDGGPSTLLNNADADYYLAVRD
                     AQEVPLQNRADLAMLLRDGYLSAYPDATLRPRQPLSRARALHAIARILEARNLLQLQK
                     ANARPASDGQLILRSAKGKDQPIRINPDAFLFRQLGERPYAVSSVTLVGGEPVTYHLA
                     ANGQIDFLEVKPAPNGAAADRFSPFSSWTKGLSLAQVQARLARAARGIGPIVDLRVVS
                     RGSSHRVTD"
BASE COUNT          390 a          588 c          659 g          424 t
ORIGIN      
        1 aatcagtcac gcggtgcgac gaacctcgcg aaacaacgcg cagatctacg attggtccaa
       61 tgccgcgcgc cgccctcgcc agccgcgcct gcacctgggc cagcgagagt cctttggtcc
      121 aactcgaaaa cggcgagaaa cgatctgcgg cagcgccgtt tggcgccggt ttgacttcga
      181 gaaaatcgat ttgaccgttt gccgccagat gatacgtgac gggctcgccg ccgacgagcg
      241 ttactgacga aaccgcgtag ggacgttcac cgagctgtcg aaacagaaac gcatccggat
      301 tgattcggat aggttggtct tttcctttcg cggaacggag gatcaactgc ccgtcgctgg
      361 cgggccgggc gtttgctttt tgcagttgca acagattccg cgcttcgagg attcgcgcga
      421 tcgcgtgtag cgcgcgggcg cgagacaaag gctggcgtgg ccgcaaagtg gcgtcaggat
      481 acgcggagag gtagccgtcg cgcagcagca tcgcgagatc ggcgcggttt tggagcggca
      541 cctcctgcgc gtcgcgcacg gcgaggtagt aatcggcatc ggcgttgttt agcagcgtcg
      601 agggtccgcc atcgccgaag acggccgcca agagggcggt cgcgaatggc ggtgggcgat
      661 tcacatcgtc gccggtgacg ggcatttttt gacgtgccac ccgcgccacg ttggcgatcc
      721 agttgcgcac ttcggaagcg gatgcgtccg ctgcaagcca cgcgtctgaa actcgttcct
      781 gcaacgtgcc gaagttttgt gtcagcagaa gactgacgtc gcgaaccagc gctacatgtt
      841 cgtcatcgcg caactcggca ggtgtgcggg tggtgcgaat cacgaaacgt tcaaaagcgg
      901 cgttgccttc agcggcgcac tcgcgcccac ggaggtaagg aagcgcatgg ttgaagatgt
      961 tttctgaatc ttcggtgcga ccgccgcagg ttgacgtgta gagcgcattg atcggttcac
     1021 ctgcgaaagt ggcgacgagg ccgcgtgttt catcgaccgc tcgggttgac agtgggttct
     1081 cagaagtgag accgcgatag acttgcgagc gcgttgtggg caggatgtcg aaaccctggg
     1141 ccataaattg accgcggttt ttgagtgcgt acgtgcgcgc ggcgatggcc tgtgctttca
     1201 atgcctcgat ggctggatag ccgccgggtg agagttcgtt cgcgacaacg ccgcgaacgt
     1261 aatcttccag gccgagctcg ttgacgaccg tgagtgtgcc gcggaggttc gtgaacacct
     1321 cgatccggcc acgatagggc cgatcgttga accgcacggg cgcatctttt tcgttgtctg
     1381 aagcaaaaac gaccggcgcg ctcgaactga acatccggcc agcggtcgag gacgcaacca
     1441 cttcacgtga cggcgagctc gcgcgtgaag tcagctttac gctaccagta gactgcgagg
     1501 actgcgttgc tcgcaaagtt tccgttttgt cggtgtaaat cggcggcgct tgtacgtccg
     1561 catccaaacc cgcagcttca agtcgcgcgc gcaactcttc cgcctcttca cgcgaacgct
     1621 tctcaccaac agccacgccc cacgtcttgg tttctgtgtc aaacgtttcc tgcgtgtctt
     1681 cgccggtaag cttctcgatc tctttagcgc tggcttcagc ttcatcgtgc gtcgccgcgc
     1741 ccgccacgat cacgcgaaac atcgcgtcgt tgtttgcggg caatggcgac aacagccgtg
     1801 catcgacgcg gacgcgcgaa gtatcaagcg cgagcagcgt ggtaccgccg ccggtggcgt
     1861 tcatcagatg acccttcgtc gaaatcaccg cagaacgcac gtcggttgtc agggcgacgc
     1921 gaatcttcgg ttcaggaccg gaaacagcag cgagagttgg cgtagagatg attggtgtag
     1981 acgccgatgg ttgcggccac gcgggttgag ttctgcgcgg gcgagtttct gtttgagttt
     2041 gctgttgagc gaacgttaat g
//
LOCUS       DASTEY010000354         3066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1013110, whole genome shotgun sequence.
ACCESSION   DASTEY010000354 DASTEY010000000
VERSION     DASTEY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013110"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1647)
                     /locus_tag="VFO72_06225"
     CDS             complement(289..1647)
                     /locus_tag="VFO72_06225"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HET9478920.1"
                     /translation="MSTGVSAPPQRPQIKTALPGPNAKKIIEADAKFVNPAYPRPEYK
                     LVAERASGVWVEDVDGNLFLDCNAGVAVCSTGHCHPEVVQAIQKQAAELIHMCGTDYY
                     YRQMPELAEKFDAIVPVQAPTRTHFANSGTEAVETALKLAMHATGREKFIAFFNSFHG
                     RTLGSLSLTSSKVAQRRGFKRQALDVVHVPYPNEFRNPFSAADCGDGGAAQGALNWIE
                     ERLFKTTTPPEEVAAIVVEAVQGEGGYVPAPKGFLQGLRSICDRHGILLIVDEVQSGM
                     GRTGKMFACEHYDVKADIVCLAKGIASGLPLGACVSRADLMNWKPGAHASTFGGNPVC
                     ISAALKTIELLEGGLIQNAADVGDYLKAGLERLKAKHECIGDVRGMGLMIGVEFVENK
                     TSLKPAPELRDRIEVACFERGLIILGAGTNTIRWSPPLVLTKENVDVALEIFDEAIAA
                     AV"
     gene            1898..>3066
                     /locus_tag="VFO72_06230"
     CDS             1898..>3066
                     /locus_tag="VFO72_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478921.1"
                     /translation="MKIHWKQNRFALVFFALLLSLAVVAPFLSHASDDKKDPKGKIVY
                     PETKKGEVVEEYFGTKVPDPYRWLEDETSAETKAWIDAQNKVTFAYLDKIPYREKLKA
                     RLTELYNYPRISAPFRRGDTYFFTKNDGLQNQSVYYIQKGVNGTPEVFLDPNKFSADG
                     TSVLSAFSLSKDGKYVAYGISQGGSDWVTLSVMEVATRKKLDDEVHWLKASGVSWRGD
                     GFYYSRYPSPEKGRELSSKNEFQTVYYHKVGTPQSEDVLIYEDKQNPQRFQNVGTTED
                     ERFAILSISERGKGKKGNAIFYKDLSKPESKFISIVAEIGDDNFGVIDNVGDKFLIRT
                     NRNAPNGRVVLVDPQNPDEKNWKDVLPERSEPLQSAGTAGGKLFASWRKDVATRA"
BASE COUNT          705 a          902 c          794 g          665 t
ORIGIN      
        1 tgcggctaga cagtacctca ttaacaggcg attgatagct tcggatcgtg tgattgccat
       61 cgatggcggg ttccgagaaa tggctcaata tgacttgttt tcactcagct atgagatgcc
      121 tcctccgagt ccgactccta ctgtcccctc taacgaggtg caaatcgtta gacctacgac
      181 gcgtcccaaa cggccctcgc gtaaataacc cgcatgtaga cacgtatacg atggtccgcg
      241 ataatccata accagtacat ccacgacttc cgcaaactca ccaccattct aaactgccgc
      301 ggcgatcgcc tcgtcgaaga tctcgagcgc gacgtcaacg ttctccttcg tcaacaccag
      361 cggcggtgac cagcggattg tgtttgtgcc cgcgccgaga attatcaagc cccgctcgaa
      421 acaggccacc tcgatccgat cgcgcaattc cggcgcaggc ttcaacgaag ttttgttctc
      481 cacaaactca actccgatca tcaaacccat tccccgcaca tccccaatac actcgtgctt
      541 cgccttcagt cgctccaatc ctgctttcaa ataatctccc acatcagcag cgttctggat
      601 caatccccct tcgagcaact caatcgtctt caatgccgcc gaaatacaaa ccggattgcc
      661 gccaaacgtt gacgcatgtg cgccgggctt ccagttcatc agatccgcac gcgacacgca
      721 cgcgccaagt ggcagacccg acgcaattcc tttcgcgaga caaacaatgt ctgccttcac
      781 atcgtagtgc tcgcacgcga acatcttgcc cgtgcggccc atccctgatt gcacttcatc
      841 gacaatcaac agaatcccgt gtcgatcgca gatgcttcgt agtccctgga gaaaaccttt
      901 cggcgcaggc acgtaaccac cttcgccctg tactgcctcc accacaatcg ccgccacctc
      961 ttcgggtggc gtcgttgttt tgaacagccg ttcctcgatc cagttcaggg cgccctgtgc
     1021 cgccccgccg tcgccgcaat cagccgcgct aaacggattg cgaaactcgt tgggatacgg
     1081 cacgtggacc acgtcgagcg cctgccgctt gaaacctctt cgttgtgcaa ccttcgatga
     1141 agtcaacgaa agtgagccca acgtgcgtcc gtgaaaacta ttgaagaacg cgatgaactt
     1201 ctcgcgtccc gtcgcatgca tcgcgagctt cagcgccgtt tcgactgctt cagtgccgct
     1261 gttggcaaag tgtgtccgcg tcggcgcctg caccggcacg atcgcgtcga acttctccgc
     1321 gagctccggc atttgtcggt agtaataatc agtgccgcac atgtgaatta gttcagccgc
     1381 ctgcttctga atcgcctgga ccacttcggg atggcaatgc ccggtggaac agactgctac
     1441 gcccgcattg caatccagaa aaagattccc atccacatct tcaacccaaa cgccgctggc
     1501 gcgctcggcg acgagtttgt actcgggccg gggataggcg gggttaacaa atttggcatc
     1561 cgcttcgatg atcttctttg cattcggtcc cggtaatgcg gtcttgatct ggggcctttg
     1621 gggtggtgcg cttacgccgg tcgacatttt cttttctcct tgtccaaaac gactattgca
     1681 ggtcggaaaa ccgaagtttt cagacgttat cagggtgcag gttatgaaga cgagatggct
     1741 agtcggcgaa gagattcgcg cgcgactcga gcgggcggta atgcaatggt tggagattaa
     1801 tcacgggagc gattctagca gccgcggttt gcctcggaca agctccggtt gttaaagtag
     1861 gcggccaaat catacctgcc tgttggagat taaaaacatg aagatccact ggaaacagaa
     1921 ccgcttcgcg cttgtgtttt tcgcgctcct tctttcgctc gccgtcgtgg cgccgtttct
     1981 gtcgcacgct tcggacgaca agaaggatcc gaagggcaaa atcgtctacc cggagacgaa
     2041 gaagggcgag gtcgtcgagg agtatttcgg caccaaagtg cccgacccgt atcgttggct
     2101 cgaagacgaa acctctgccg agacgaaagc ctggatcgac gctcagaaca aagtcacttt
     2161 cgcctatctc gacaagatcc cttatcgcga gaaactcaaa gcgcggctga cggagcttta
     2221 caattacccg cgcatctccg cgccgttccg acgtggtgac acttactttt tcaccaagaa
     2281 cgacggcttg cagaaccaga gtgtctacta cattcaaaaa ggcgtgaacg gcacgccgga
     2341 agtcttcctc gatccgaaca aattctccgc tgacggcacg tcagtgttga gcgcgttctc
     2401 actttcgaaa gacggcaagt acgtcgcgta cggcatctcg cagggcggtt cagactgggt
     2461 cacgctcagc gtgatggaag ttgcgacccg taagaaactg gatgacgaag tccattggtt
     2521 gaaagcctca ggcgtgtcgt ggcggggcga cggcttttat tacagccgtt atccgtcacc
     2581 ggaaaagggc agggaactct cctcgaagaa tgaattccaa actgtttact accacaaggt
     2641 cggcacgccg caatctgaag acgttttgat ctacgaagac aagcagaatc cgcaacgctt
     2701 tcagaacgtg ggcaccacgg aagacgagcg gttcgcgatc ctgagcatct ccgaacgtgg
     2761 caagggtaaa aaaggaaacg cgatctttta caaggacttg tcgaaacctg agtcgaagtt
     2821 tatttcaatc gtggctgaga ttggcgacga taatttcggc gtcatcgaca acgttggtga
     2881 caagtttctg attcgcacga atcgcaatgc gccgaacggt cgcgtcgtgc tcgtcgatcc
     2941 gcagaaccct gacgagaaga attggaaaga cgtgttgccg gaaagaagcg agccgctgca
     3001 aagtgcgggc acggccggcg gcaagctctt cgcctcctgg cgcaaggacg tggccacgcg
     3061 cgcgta
//
LOCUS       DASTEY010000355         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_996803, whole genome shotgun sequence.
ACCESSION   DASTEY010000355 DASTEY010000000
VERSION     DASTEY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996803"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2256)
                     /gene="uvrA"
                     /locus_tag="VFO72_06235"
     CDS             complement(<1..>2256)
                     /gene="uvrA"
                     /locus_tag="VFO72_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9478922.1"
                     /translation="FKKELEKLAKEGFLRARVDGELLSLDEEIRLDKRRNHTIEAVVD
                     RLLIKTGIRERLTDSIRTALKLTGGAVLISIVDGEEKLYSERMACVNCGINVPPLEPR
                     SFSFNSAYGACKRCHGLGTVMEIDSLKLIPDARQTPEKLGYMGTADKQGSAYLRTALL
                     AVCKHFNCDPKTPFADLSKEVREGFLNGVNGKITFVQGLYTYQSEWKGALKWLQERLS
                     EAPSEKVRVALEELVSPTVCSECNGRRLRSDSLSVQVGRRGIAEYTALPIEAAVEAFE
                     QVQLNPREEQIAGPILREIKNRLRFLKTVGLGYLTLDRSSATLSGGEGQRIRLATQIG
                     SQLRGVLYVLDEPSIGLHPRDNQKLLDTLSALRDLGNTVLVVEHDEETIRRADYVVDL
                     GPGAGAHGGQVVAVGTAADVAENPESITGLYIRGARKIEVPAERRAPNGKYLTITGAY
                     ANNLKEIDVSFPLGLLTVVTGVSGSGKSTLVEDILYYALAQHLYGSLTEPGPYKAIEG
                     LEHVDKVIEIDQSPIGRTPRSNPATYTGLFTPLRELYAMLPESRERGYKPGRFSFNVK
                     GGRCEACEGDGMKRIEMNFLPDVYVLCDVCRGARYNRETMAVKYKGKSIADLLDTTVE
                     EALPLLENIPQINQKLQTLLDVGLGYIKLGQSATTLSGGEAQRIKLAKELSKRATGRT
                     IYILDEPTTGLHFADVHKLLDVLQRLVSLGNTVLVIEHNLDVIKSADYIIDLGPEGGE
                     HGGRVVAAGTP"
BASE COUNT          445 a          669 c          628 g          514 t
ORIGIN      
        1 cgggagtgcc ggcggcgacg acgcggccgc cgtgttcgcc gccttctggt ccaaggtcga
       61 tgatgtagtc ggctgatttg attacgtcga gattgtgttc gatcaccagc accgtgtttc
      121 ctagcgacac caggcgctgc aggacgtcga gcagtttgtg aacgtccgcg aaatggaggc
      181 cggtggtggg ttcgtcgagg atgtagatcg tcctgccggt ggcgcgcttc gagagttcct
      241 tggcgagttt gatgcgttgt gcctcgccgc cggaaagcgt cgtcgccgat tggcctaact
      301 tgatgtaacc gagacccacg tccagcaacg tttgcagctt ctgatttatc tgcggaatat
      361 tttcgagcag cggcagtgct tcctcgactg tcgtatccag caggtcggcg atcgacttcc
      421 ctttatactt cacggccatc gtctcacggt tgtagcgcgc gccgcgacac acgtcacaca
      481 gcacgtacac atcaggaaga aagttcatct cgatgcgctt catcccgtcg ccttcgcacg
      541 cttcgcaacg tccgcctttg acgttgaaag agaaacgtcc cggtttgtaa ccacgctcac
      601 gcgattcggg tagcatcgca tagagctcgc gtagcggcgt gaataagcct gtgtaggttg
      661 cgggattcga tctcggcgtg cggccaatgg gcgactggtc gatctcgatg accttgtcga
      721 cgtgttcgag tccctcgatg gctttgtacg gccccggttc tgtcaacgaa ccgtacagat
      781 gctgcgcaag cgcgtaataa aggatgtctt caacgagcgt cgatttaccg gaaccggaaa
      841 cacccgtcac caccgtcaac aagcctaacg gaaacgaaac atcgatctcc ttgagattgt
      901 ttgcataagc accggtgatc gtcaggtact tgccgttggg ggctcttctt tcagccggga
      961 cctcgatctt tcgcgcgcca cgaatataca gcccggtgat cgactccgga ttttccgcga
     1021 cgtccgctgc ggtgcccacg gcgacgactt gtcctccatg cgcgcccgcg cctggtccaa
     1081 gatcgacgac gtaatcagcg cgcctgatcg tctcctcgtc gtgttcgact acgagcactg
     1141 tgttgccgag atctcgcagt gcagaaagtg tgtcgagcag cttctgattg tcgcgcggat
     1201 gaagtccgat tgagggctcg tcgagcacgt acagcactcc gcgcagctgc gaaccgatct
     1261 gcgttgcgag ccgaatgcgt tggccttcgc cgcctgaaag tgttgccgac gatcgatcga
     1321 gcgtcaggta gcccaggccg acagtcttca agaagcgcaa acggttcttg atctcgcgca
     1381 ggattggacc ggcaatctgt tcttcacgtg gattgagctg cacctgttcg aatgcttcga
     1441 ccgcagcctc gatcggcaac gccgtgtact cggcaatgcc gcgcctgccc acctggaccg
     1501 acagcgaatc ggaacgcaag cgccgcccgt tacactcgct acaaaccgtc ggggaaacca
     1561 actcctcgag cgccactcgc actttttccg aaggcgcttc gctcaatcgt tcctgcagcc
     1621 acttgagcgc gcccttccat tcgctctgat aagtgtaaag tccctggacg aaagttatct
     1681 ttccgttaac tccattcaaa aacccctcgc ggacttcctt cgacaggtcg gcaaacggcg
     1741 tcttgggatc gcagttgaag tgtttacaga ccgcgagcag ggccgtgcga aggtaagcgg
     1801 agccttgttt atcggcggta cccatgtagc cgagcttctc gggcgtttgg cgtgcgtcgg
     1861 gaattagttt cagagaatcg atctccatga ccgttcccag accatgacat cgtttgcacg
     1921 cgccatacgc ggagttgaaa gagaaggagc gcggctcgag tggcggcacg ttgatgccgc
     1981 agttcacgca ggccattcgt tccgagtaaa gcttctcttc gccgtctaca atcgaaatca
     2041 gcactgcgcc accagtcaac ttcaacgcag tccgaatcga atcagttagt cgttcacgaa
     2101 tgccggtctt gattaacagc cgatccacga ccgcttcgat cgtgtggttc cggcgtttgt
     2161 cgaggcggat ctcttcgtcc agcgaaagca actcaccatc gacgcgcgcg cgcagaaatc
     2221 cttctttcgc aagtttttcc agctctttct tgaact
//
LOCUS       DASTEY010000356         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_139721, whole genome shotgun sequence.
ACCESSION   DASTEY010000356 DASTEY010000000
VERSION     DASTEY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139721"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFO72_06240"
     CDS             <1..341
                     /locus_tag="VFO72_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478923.1"
                     /translation="LARHIGAFHLFSTSVGKYTISRAGWGEVYVGDRKKNFRVKVGAR
                     ERIERLYFFEYEGDLLLLYEAGASGYLLRLHQKTRKVRRATSINEDFEPPLVKDQSLT
                     FSDGTVVPLN"
     gene            complement(334..771)
                     /locus_tag="VFO72_06245"
     CDS             complement(334..771)
                     /locus_tag="VFO72_06245"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9478924.1"
                     /translation="MAEGKTKPTRQSVTEFLNKIPDAGRREDCFAIAKMMQEITGSKP
                     QMWGTSIVGFGSYHYKYASGHEGDWPLTGFSPRKQDLTLYVMMGFEKYEALMNQLGKH
                     RKGKACLYIKRLSDIHVPTLKKLIKTSVKDLQARTKSQRPAIN"
     gene            complement(752..2380)
                     /locus_tag="VFO72_06250"
     CDS             complement(752..2380)
                     /locus_tag="VFO72_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9478925.1"
                     /translation="MHKLLFRVVLIALCVAALMTAPHSSTATPPSLIDDYKAQVVADV
                     ASMAKQSQVMVDMIFSFGELGFQEVETSKYLTGILEKEGFKIERGVAGIPTAWVATWG
                     SGKPVIALGSDVDCIPQASQKPGVAYHDPLVDGAPGHGEGHNSGVPLTITAAIAVKRQ
                     MQRAKLPGTIKLWPGVAEELVAGKAFFVREGVFKDVDVVLFTHVGNNLNTSWGQSSAT
                     GLISVEYTFRGESAHSAGAPWQGRSALDAVELMNVGWNYRREHLRLQQRSHYVITNGG
                     DQPNVVPRLASVWYYFRELDYEHIKQMREIGDAMAQGAALMTNTNVTSRVLGSAWPQH
                     FNRPVAEAVYENIKQVGLPQWSEADQKLAKALQRELKGREQGLAKELMPLAGPVTDEQ
                     RTRGGSDDIGDVSWNVPTVSLRYPSNIPGLPGHNWSNAIAMATPIAHKGVTAGAKVVA
                     MTMIDLLTKPELIEQAWKYFREVQTKDRKYQPLISADDKPPIWLNKRIMDSYRDQMRK
                     FYYNPDKYDTYLEQLGIKYPTVREGATQSGGRQD"
     gene            complement(2350..>3337)
                     /locus_tag="VFO72_06255"
     CDS             complement(2350..>3337)
                     /locus_tag="VFO72_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9478926.1"
                     /translation="LERAERTALAIALPLEIFYRTFRWPIRLLDWAGTRTVRLFGLHP
                     SSGHASIYTEEELRQLIDISHQSGHLQADEQRLINRIFDFSDAQVREAMIPRTSIAAL
                     PVTATLEQTREALRSLGYSRLPVYHGVLDNVVGVLVRRDFEPYLDTTTAPAKFDLREL
                     IHTPHFVAATARLDVALKQMQSKRSHLAFVVDEHGAIEGIVTLEDLLEEIVGEINDEF
                     DDEVREQITREPDGSYSLSGILTVRDANRQLKLELPEHSGYTTLAGFLMAQAGHLPQI
                     GEVVQYEDLTFQIERMEGRRIRRVRLTSAVTAAILLAVLGDATCINFCSELF"
BASE COUNT          694 a          962 c          915 g          766 t
ORIGIN      
        1 cactggcgcg acacatcggc gccttccatc tcttttccac atccgttggc aagtacacca
       61 ttagtcgcgc tggatgggga gaagtctacg tcggcgatcg taagaagaac ttccgggtaa
      121 aagtcggcgc gagagaacgt atcgagagac tttacttctt cgaatacgaa ggcgacctgc
      181 tgttgctgta cgaagctggc gcatccggat atttgctgcg actgcatcag aagaccagaa
      241 aagtaagaag agcgacctcg atcaatgaag atttcgagcc gccgctggta aaagatcaat
      301 cgctgacttt cagtgacggc acggtcgtcc cactcaatta atcgccgggc gttgcgactt
      361 cgttcgagcc tgtaggtcct tcacggacgt cttgatcagt ttcttgagcg taggcacatg
      421 gatgtcggaa agccgtttga tatacaaaca tgccttacct tttcggtgct tacctaactg
      481 gttcatcagt gcttcgtact tctcaaagcc catcatcacg tagagcgtca gatcctgttt
      541 gcgaggcgag aaaccggtca atggccagtc gccttcatga ccgctggcat acttgtagtg
      601 gtaactgcca aatccgacga tagacgttcc ccacatctgc ggcttgctgc cggtgatctc
      661 ctgcatcatc ttcgcgatgg cgaaacagtc ctcacggcgt ccagcatcag gaatcttgtt
      721 gaggaactca gtaacactct gcctggtcgg tttagtcttg ccttccgcca ctctgtgtcg
      781 ctccttcccg caccgtcggg tatttgatcc ccagctgctc gagataagtg tcgtacttgt
      841 cagggttgta ataaaacttc cgcatctgat cgcgatacga atccatgatt cgcttgttga
      901 gccagatcgg cggcttgtcg tcagcggaga tcagcggctg atacttcctg tccttcgtct
      961 gcacttcccg aaaatacttc cacgcctgtt cgatcaactc cggtttggtc aacagatcga
     1021 tcatcgtcat cgccacgact ttcgcgccgg cagtgacgcc cttgtgcgcg atcggggtcg
     1081 ccatcgcgat cgcattgctc caattgtgac cgggcaaacc aggaatgttc gacggatagc
     1141 gcaacgagac agtcggcacg ttccaggaga cgtcgccgat atcgtccgag ccgccgcgag
     1201 ttcgctgttc gtcggtaact ggtccagcca acggcatcag ttctttcgca agaccctgct
     1261 cgcgtccttt cagttctcgt tgcaacgctt tcgcgagctt ctgatcggct tcgctccatt
     1321 ggggcagacc aacctgtttg atgttttcgt agaccgcttc cgcgacgggc cggttgaagt
     1381 gttgcggcca agcggaaccc aaaacgcgag aagtcacgtt cgtgttggtc atcagcgcag
     1441 cgccctgggc cattgcgtcg ccaatctcgc gcatctgttt gatatgttcg taatcgagtt
     1501 cgcggaagta gtaccaaaca ctcgccagcc gcggcacgac gttgggctga tcgccgccgt
     1561 tagtgatcac gtaatgcgaa cgctgttgga gtcgcaagtg ttcgcgcctg tagttccacc
     1621 cgacattcat caactcgacc gcatcgagag cgcttcgtcc ctgccacggc gcaccggccg
     1681 agtgagcact ctcgccacga aacgtgtatt caactgaaat cagaccggtc gcggaagatt
     1741 gtccccagga ggtgttgaga ttgttgccga cgtgcgtgaa cagcaccacg tccacgtctt
     1801 tgaaaactcc ctcacgaaca aagaacgcct tacccgcgac gagttcttcc gccactccgg
     1861 gccacagttt gatcgtgccc ggcagtttgg cgcgttgcat ctgtcgtttc actgcgatgg
     1921 cggctgttat ggttagcggc acgccggagt tgtgcccttc gccgtgtccg ggcgcgccgt
     1981 caaccagcgg atcgtgataa gcgacgcccg gtttttgtga agcctgagga atgcaatcaa
     2041 cgtcggatcc gagcgcgatg acgggtttac ccgatcccca cgttgctacc cacgcggttg
     2101 gaattcccgc tacgccgcgc tcgatcttaa agccctcttt ttcgagaatg ccggtgagat
     2161 acttcgaagt ctcaacttcc tgaaaaccga gttcgccgaa actgaagatc atgtcgacca
     2221 tcacttgcga ctgtttcgcc atcgacgcga cgtcagccac aacctgcgcc ttgtagtcgt
     2281 caatcagcga cggaggcgta gcggtagagg aatgcggcgc ggtcatcaac gccgcgacgc
     2341 acagcgcaat cagaacaact cggaacagaa gtttatgcat gtggcatctc ccagaacggc
     2401 caacaggatg gccgccgtca cagcggatgt gaggcgaacg cgacgaatgc gccgtccttc
     2461 catccgttct atttggaacg tcaagtcttc gtactggacc acttcgccga tttgcggcag
     2521 atgtccggcc tgggccatga gaaagcccgc gagtgtcgtg tagccgctat gttcgggcag
     2581 ttcgagtttc aactggcgat tcgcatcgcg cacagtaaga ataccgctca acgaatagct
     2641 gccgtcaggt tctcttgtta tttgctcgcg cacctcatcg tcaaactcgt cgttgatctc
     2701 gccgacaatc tcctcgagca agtcttccag ggtcacgatg ccttcaatgg cgccgtgctc
     2761 atccacgacg aaagcgaggt gagatcgttt cgactgcatc tgctttagcg ccacgtccag
     2821 acgtgccgtg gcggcaacga aatgcggcgt gtgaatcagt tctcgcagat cgaattttgc
     2881 cggcgcggtc gttgtgtcga gataaggctc gaagtcgcgt cgcacgagaa cgccgacaac
     2941 gttgtcgaga acgccgtgat acaccggcaa ccgcgaatat ccaagactgc gaagcgcttc
     3001 cctggtctgt tccaaagtgg cggtcaccgg cagcgcggct atggaagtgc gcggaatcat
     3061 tgcctcgcgc acctgcgcgt cggaaaaatc aaagatgcga ttgatcaggc gttgttcgtc
     3121 ggcttgcagg tggccactct gatgtgaaat gtcgatcagt tggcgtagct cttcttcggt
     3181 gtagatcgaa gcgtgtccgc ttgacgggtg caagccaaac aggcgtacag ttcgcgttcc
     3241 cgcccagtca agcagtcgta tgggccagcg aaacgtgcgg tagaagatct ccaacggcaa
     3301 agcgattgcc agcgctgtgc gttcggcgcg ttcgagt
//
LOCUS       DASTEY010000357         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_799236, whole genome shotgun sequence.
ACCESSION   DASTEY010000357 DASTEY010000000
VERSION     DASTEY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799236"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFO72_06260"
     CDS             <1..535
                     /locus_tag="VFO72_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478927.1"
                     /translation="VVERGTARKARGAIPGTAIAGKTGTSRDGWFVGYSPNLVCVVWI
                     GFDDNSQLGLTGAEAALPAWVDFMRGAIAVRPDLGGSNFECPEGIKFVEIDSATGLIS
                     TLSCPLRELIAVTEAKGPHLECYLHGNVPVQTSPFAEESEATMRTQVVEQRGQGFTRI
                     ETDLTGKKTLMNEITVR"
     gene            complement(543..920)
                     /locus_tag="VFO72_06265"
     CDS             complement(543..920)
                     /locus_tag="VFO72_06265"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET9478928.1"
                     /translation="MIVSIGIDIIEVARIREVLLRTPRFTERVFTRAERAYCDSRGVV
                     APQHYAARFAAKEAALKALQTGWRGGISWQDMEVSAHDSGAPYLIFTGEVLAIYEKFG
                     ATAAHLSLSHTSEHAIAQVILER"
     gene            901..1776
                     /gene="pgeF"
                     /locus_tag="VFO72_06270"
     CDS             901..1776
                     /gene="pgeF"
                     /locus_tag="VFO72_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET9478929.1"
                     /translation="MPMETITSDEHLIQAGFYWRELNGLRALVCRPLEQDGFVNGFST
                     RLGGVSSMPHESLSLAGFHDDAAENILENRRRFLKLFPGHWTLAGCWQVHGADVRVVG
                     SVEDAKPAENQLGETVYCDVIVSNADGVLAGVKTADCVPILIGDPRTRAFAAVHAGWR
                     GTLESAAIAGVKRLSEEYGARPEELRVAIGASAGPCCYEVGAEVIDAFSSRFANGANL
                     FTPTRPGHGLIDLLKANRDQLESIGVLSEKIHIAPICTMCRTDLFFSYRKEKTLNGKV
                     GRLMAVIGRNQVTAR"
     gene            complement(1763..2254)
                     /locus_tag="VFO72_06275"
     CDS             complement(1763..2254)
                     /locus_tag="VFO72_06275"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HET9478930.1"
                     /translation="MTIYSKVLSVFLLLLLPTMLYSNPRMQSSADVNVSGSIAPDKVK
                     KGRTVRGTVVLDIPSGLHVQSNKPLDKYLIPTKLEIETPAGMKVGPVTYPRAVMRKLK
                     FSKGAVAVYEGRAVVRFGVTVPANYNGSSGEIKGKLRFQACNDDACFPPVTREVKMWL
                     NVQ"
     gene            complement(2254..>2754)
                     /locus_tag="VFO72_06280"
     CDS             complement(2254..>2754)
                     /locus_tag="VFO72_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478931.1"
                     /translation="IVPRILDLAKVPDRPHLAISLAAPTDELRNELMPVNKRWPLEEL
                     LKACKVFEQTLKPAERFTFEYVMLAGVNDSEELARQLANLLNRHGLKTKVNLIPHNPA
                     EPLPYRPSSDETVARFKSILESKGIHTYVRRPRGRDIFAACGQLAARQETLNTCKEIT
                     STGVTS"
BASE COUNT          573 a          672 c          857 g          652 t
ORIGIN      
        1 cgtcgttgag cgcggtactg cgcgaaaggc tcgtggcgcg ataccgggaa cggcgatcgc
       61 cggcaaaacg ggaacgtcgc gggatggatg gtttgtgggg tactcaccaa atctcgtttg
      121 cgtagtttgg atcggtttcg acgacaactc gcaactcggg ttgaccggtg cagaagcagc
      181 gttgccggcg tgggttgact tcatgagggg cgcaattgca gtcaggcctg atcttggtgg
      241 ttcaaacttc gagtgtccgg aggggattaa gtttgttgag atcgattcgg cgacgggatt
      301 gatctcgacg ttaagttgtc cgttgcgtga gttgatcgcg gtaactgagg cgaaagggcc
      361 gcaccttgag tgttatctgc atgggaacgt gccggttcag acgagtccgt ttgctgagga
      421 aagtgaagct acgatgagaa ctcaagtcgt agagcaaagg ggacagggat ttacacgaat
      481 agaaacggat ctcaccggaa agaaaacgct gatgaatgaa atcaccgttc gttagcgatc
      541 aatcaacgtt ctaaaatgac ttgggcgatc gcgtgttcgc tggtgtgtga gagcgaaaga
      601 tgcgctgcgg tggcgccaaa cttctcataa attgccagca cttctcccgt gaagatcagg
      661 taaggcgcgc cgctgtcatg cgccgaaact tccatatcct gccaactgat tccacctcgc
      721 cagcccgttt gcagtgcttt caaagctgct tcttttgccg caaagcgcgc ggcataatgc
      781 tgcggggcca cgacgccgcg actgtcgcag tatgcgcgct cggcgcgtgt gaatacccgc
      841 tcagtaaaac gaggcgtgcg caacagcact tcgcgaattc gcgcaacttc gataatgtct
      901 atgccgatgg aaacgatcac gagtgacgaa cacttaatcc aggctggctt ttactggcgc
      961 gaacttaacg gtcttcgtgc tcttgtttgc cggccgttgg agcaggacgg ttttgtaaac
     1021 ggcttctcga cgcgtctcgg cggcgtcagc agtatgccgc acgaatcgct gagcctcgct
     1081 ggttttcacg atgacgcggc ggagaacatt ctggagaatc gtcgccgctt tctcaagctt
     1141 tttccgggcc attggaccct tgccggctgt tggcaggtgc acggcgcgga cgttcgcgtg
     1201 gtcggatcgg tcgaagacgc caagccagcc gagaatcaac ttggcgagac agtctattgc
     1261 gacgtgattg tcagcaatgc cgacggagtt ctcgcgggcg tcaagacggc tgattgtgtg
     1321 ccgattctga tcggcgatcc gcggacgcgt gcgtttgctg cggtgcatgc cggctggcgt
     1381 ggaacgctcg agagtgcagc aatcgcgggt gtgaaacgtc tgtccgaaga gtatggcgcc
     1441 agaccggaag aactgcgtgt cgcgatcggc gcttcggctg gaccatgttg ttatgaagtt
     1501 ggcgccgagg ttattgatgc gtttagtagt cgcttcgcga atggcgccaa tctgtttacg
     1561 ccgacgcgtc ccggacatgg gctgatcgat ctgctgaaag cgaatcgaga tcaacttgaa
     1621 tcaatcggag tgctgtcgga gaagattcac atcgcgccga tctgcacgat gtgccgcact
     1681 gatcttttct tctcgtatcg gaaagagaag acgttaaatg gaaaagtcgg gcggctgatg
     1741 gcggtgatag gccgtaatca ggtcactgca cgttgagcca catcttcact tcgcgtgtta
     1801 cgggcggaaa acacgcgtca tcgttacacg cctgaaatcg tagtttgcct tttatctctc
     1861 cggatgagcc gttgtagttg gcgggtacgg tgacaccgaa acggaccacc gcacggccct
     1921 cgtaaacagc gacggcgcct ttggaaaact tcagcttgcg catcacggca cgtggatagg
     1981 taactggtcc aactttcatt ccagcgggtg tctcaatctc gagtttggtg gggatgagat
     2041 atttatcgag tggcttgttc gactgcacgt gcaggccgga ggggatgtcc agcacgacgg
     2101 ttcctcgcac ggtgcggcct tttttgacct tgtcgggggc gatggagcca ctgacgttca
     2161 cgtccgcgga cgactgcatc cgcgggtttg agtaaagcat tgtaggcagc aacaagagga
     2221 ggaaaacgga taagactttc gagtagatgg tcattaactg gtaactccgg tagaagtgat
     2281 ctctttgcaa gtgttaagtg tttcctggcg agccgctagt tgcccgcagg cagcaaaaat
     2341 atctcggccg cgtgggcgcc gcacgtaagt gtgtatgcct ttggactcca gaatgctttt
     2401 gaagcgtgcc acggtctcgt cgcttgacgg ccggtatgga agcggttcgg ccggattgtg
     2461 cggaatcagg tttacttttg tcttcaggcc gtgacggttt agaagatttg ccagttgccg
     2521 cgctagttct tcggagtcgt tgacgccggc gagcattacg tactcgaagg tgaaccgttc
     2581 ggctggtttg agtgtttgtt caaacacctt gcaggctttg agcagttctt ccaatggcca
     2641 acgcttgttg actggcatca attcgttgcg taattcatcg gttggcgcag ccagagagat
     2701 cgcgagatgt gggcgatcgg gaactttcgc gaggtcgaga attcgcggaa cgat
//
LOCUS       DASTEY010000358         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_634483, whole genome shotgun sequence.
ACCESSION   DASTEY010000358 DASTEY010000000
VERSION     DASTEY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634483"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2257
                     /locus_tag="VFO72_06285"
     CDS             <1..>2257
                     /locus_tag="VFO72_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9478932.1"
                     /translation="VLLLTIAVCQSSFGQVVVGRISGTVQDSNGASVPNASVKVTNNA
                     NNSERTVTTDDNGFYTVTNLPVGTYTIEAEQKGYKKALVSGQSVTADARLTVDLKLEV
                     GEVSETVEIVGAAGETVNTTSGEVARVVDQQQVQNLALNGRNYIQLLSLVPGVALLND
                     DQLELTTSLATGNQSINGNRGQTNNVTVDGGFNLQSGSNASQINNVGIDYIQEVKIQT
                     SNFSAEYGRNSGAQVSLVTRSGGNDFHGSTFYFLRNDKLDARSFFAPVRPTLRFNNFG
                     YSFGGPIFKDKLFFFGGQEWKYIRRLSSAQRRTVPSLAELNGDFSFRLRGPDGTVGTA
                     DDGVLRDPNNAANTCTAPVFTNGVITTPANRTGCFGGNNVALRNIIPTNRITADGRAI
                     ANVYQTAIGLATAFTNSPVGNNVTFQQPNPLDNREELLRSDYRINDKHTLYGRYIKDK
                     NVLIDPFGTFINTQLPTIPSLRMRPGTSIQLSHTWLITANFINEAKVTGAWVAQRIPP
                     DGDLWKRETYGFQFQQLFLGGGRFENSIPDTTVNGFASFFGAARSLVSPTTDINVSDT
                     VTWVRGNHTFKTGLSVTRNRIDQNARTTYAGQVDFNTGGNTRTSNNSFADALLGNFRT
                     YSEQALDPLGFFRFTQTEAFVADSWKVNRKLSLELGMRYQYGLPIYTQANNLANFDPS
                     RYDPAQAVTILANGNIDTTKGGNRFNGLVRAGNGVPPEELGRVPGGDSAAVQAVPTGA
                     PRGLYEGHHYIM"
BASE COUNT          526 a          702 c          591 g          438 t
ORIGIN      
        1 tgtccttttg ctcaccatcg ccgtttgtca aagcagcttc ggacaagtcg tcgtcggtcg
       61 catctcgggc acggtgcagg attcgaatgg ggccagcgtt ccaaacgcat cggtcaaagt
      121 caccaacaat gcgaacaatt cagaacgcac ggtgacgact gatgacaacg gcttttatac
      181 cgtcaccaat ctgcccgtcg gcacttacac catcgaggcc gagcagaagg gatacaagaa
      241 ggcccttgtc tccggtcaaa gcgtcaccgc ggacgcgcga ctcaccgtcg atctaaaact
      301 cgaagtcggc gaggtttcgg aaacggtcga gatcgtcggc gccgcaggtg agacagtcaa
      361 cacgacctcc ggtgaagtcg cgcgcgtcgt cgatcaacaa caggtccaga acctcgcgct
      421 caacggtcgc aactacattc aacttctttc actcgttccc ggcgtggcgc tgctgaacga
      481 cgatcagctc gagttgacca ccagtctcgc gaccgggaat cagtcgatca acggcaaccg
      541 tggccagaca aacaacgtca cggtcgacgg cggcttcaac ctgcaatcgg gtagcaacgc
      601 gagccagatc aataacgtcg gcatcgatta catccaggaa gttaagatcc agacttcaaa
      661 cttctcagcc gagtacggcc gcaactccgg cgcgcaagtc agtctcgtga cgcgcagcgg
      721 cggcaacgac tttcacggca gcacgtttta ttttctaaga aacgacaaac tcgacgcgcg
      781 ctcattcttc gcgccggtta gaccaacgct gcgcttcaat aatttcggct acagctttgg
      841 tggtccaatt ttcaaagata aactgttctt cttcggcggg caggagtgga agtacattcg
      901 ccggctttcc agcgcgcaac gacgcacagt gccgtcactc gcggaattga acggcgactt
      961 ttcttttcgc ctgcgcggac ccgacggcac ggtcggcacg gctgatgacg gcgtgctgcg
     1021 cgatccaaat aatgctgcca acacgtgtac tgctccggta ttcacaaatg gtgttatcac
     1081 cacgccggca aacagaaccg gttgtttcgg tggaaacaat gtcgcgctac gcaacataat
     1141 tcccacgaat cgtatcaccg ccgatggccg cgcgatcgcg aacgtctatc aaacagcgat
     1201 cggtttggca accgcattta caaacagccc cgtcggcaac aacgtaacgt tccagcagcc
     1261 aaatccgctc gacaatcgcg aagaactgct gcgcagcgac tatcgcatca acgacaagca
     1321 cacgctctac ggccgttaca ttaaagacaa gaacgtcttg atcgatccat tcggcacttt
     1381 catcaacact cagttgccga ccatccccag tctccgcatg cgtccgggaa cttcgatcca
     1441 gctctcgcac acgtggctca tcacggcgaa ctttattaac gaggcgaagg tcacgggcgc
     1501 gtgggtggcc caacgaattc cgccggacgg cgatctttgg aaacgcgaaa cgtacggctt
     1561 ccaattccag caactgtttc tgggcggcgg caggtttgaa aactccattc cggatacgac
     1621 cgtcaacggc tttgcgagtt tcttcggcgc ggcccgctct ctggtttcac caacaaccga
     1681 catcaacgtc agcgacaccg tcacctgggt tcgcggcaac cacaccttca agaccggctt
     1741 gagcgtcact cgcaaccgca tcgatcagaa cgcgcgcacg acatacgccg gccaggttga
     1801 cttcaacacg ggcggtaaca cgcgtacgag caataactcc ttcgccgatg cgctgctcgg
     1861 aaatttccgc acctacagcg aacaagcgct cgatccgtta ggcttcttcc gcttcactca
     1921 gaccgaagcg ttcgtggccg acagttggaa agttaatcgg aagctgagtc tcgagctcgg
     1981 catgcgctac cagtacggcc tgccgatcta cacgcaggcg aacaacctcg ccaacttcga
     2041 tccgtctcgt tacgacccgg cgcaggcagt cacgattctc gcgaacggaa acatcgacac
     2101 gaccaaaggc ggcaatcgct tcaatggtct ggttcgcgcg ggtaatggtg tgccgcctga
     2161 agagcttggg cgcgtgccgg gtggcgactc cgcggcggta caggccgtgc cgaccggtgc
     2221 gccgcgtggt ctatacgaag gccatcacta catcatg
//
LOCUS       DASTEY010000359         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_73858, whole genome shotgun sequence.
ACCESSION   DASTEY010000359 DASTEY010000000
VERSION     DASTEY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73858"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="VFO72_06290"
     CDS             <1..1922
                     /locus_tag="VFO72_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478933.1"
                     /translation="EYVHLHLRDNVPGVPVWLNEGLAELYGSLQFSGGEAVLGVPLPY
                     LRLLRSQELLPLTTLLSIDSSSPHYNEQDKTGIFYGESWALVHYLMLGGGSQRHDQFK
                     RFLQQVGRGEDAANAMQDSFGMSLDVIEKELRAYIRRGELPTLRIASGDDPQAYASYT
                     AMQRVSLSEGEANYYLGDLLLHIGRNEDAERYFKHAIALEPGLTAAYAALGQLSVRQK
                     RFEEAKKYLQRATTSPQHYLVHYHYAWLLSREAASPTGFITEYSAENAAVMREQLLRV
                     VKLAPDFAPAYYLLALVNLVRDERLDEAIEMAEKARRLQPKASYTLLLAQIHARRSEV
                     AVARSLVEPLARGSDAGVRTEAQRLLDSLNNTSSANRSSGPSTRSAARVSGALAAEPV
                     ATNTGRTLGGDSSGGTAIRDGRTIETSGSLPALDEVLARYVEAMGGADAIKAVTSRVT
                     KGTVDVPGLSRGGSFETSEQAPNKATTVIQAHPFGTIKIGYNGRNGWSKGADRVQLIK
                     DRTELAALQRDSDIYGVVRLKNNFAKVTLAGKSEIGYREVYVLDLQPAVGSVERLYLD
                     AKTYLPVRMNTVRKIGAVAEAVEIYLDDWKAVDGIQVPFSVTQRFRTLTLSYTVKEVR
                     HNVPLEPSLFEPPIK"
     gene            complement(1925..>2450)
                     /locus_tag="VFO72_06295"
     CDS             complement(1925..>2450)
                     /locus_tag="VFO72_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HET9478934.1"
                     /translation="SLGVLADTKIFLDDTPGISVLEMRAKARRLAAEQKKLDLIVVDY
                     LQLMSGSARRVESRQQEVSQISRELKGLAKELNVPLVALSQLSRAPESRSDHRPQLAD
                     LRESGALEQDADLVAFIFREEQYKSPEERDAMPEDRKNIAELIIAKQRNGPTGTVELR
                     FTPSSMRFDNLYRE"
BASE COUNT          544 a          682 c          725 g          499 t
ORIGIN      
        1 atgagtacgt gcacctgcac ctcagagaca acgtgcctgg agtgcccgtg tggctcaacg
       61 aaggactggc cgagctctat ggctcgctac aattctcggg cggtgaagcg gtgcttggcg
      121 tgccgttgcc ttatctgcgt cttttgcgta gccaggaatt gttgccgctc acgactcttc
      181 tctcgatcga ctcgagttct ccgcattaca acgaacagga caagaccgga atcttctatg
      241 gcgagtcgtg ggcgctggtc cattacctga tgctgggcgg cggctcacag cgtcacgatc
      301 agttcaaacg tttcctgcag caagtgggcc gtggtgaaga cgctgccaat gcaatgcagg
      361 actctttcgg catgagtctt gacgtcatcg agaaagaact acgcgcgtac atacgtcgcg
      421 gagagttgcc gaccttacgc atcgcaagcg gcgacgaccc gcaagcttac gcatcctaca
      481 cggcgatgca gcgcgtgtcg ctctctgaag gcgaagcgaa ttattacctc ggcgatttac
      541 tgctgcacat cggacggaat gaggatgccg agcgttactt caaacacgcg atcgcgctgg
      601 aaccgggatt gacagccgct tatgccgcgt tgggccaatt gagcgtgcgc cagaaacgct
      661 ttgaggaagc aaagaagtat ttacaacgcg caacaacatc accgcagcat taccttgttc
      721 attatcacta tgcgtggttg ctgagtcgcg aagcagcctc gccaaccggc ttcattacag
      781 aatattcagc cgagaatgcc gcagtgatgc gcgagcaact gttgcgtgtc gtcaaactcg
      841 ctccggattt tgctccggcg tactacctgc tggcgctagt caatctcgtg agagacgagc
      901 gcctcgatga agcgattgag atggccgaga aggcgcggcg cctgcaaccg aaagcgtctt
      961 acacgctgtt gctggcgcag attcacgccc gtcgatcgga agtcgcggtt gcgcgttcgt
     1021 tagtagaacc actcgcgcga ggctccgacg cgggagtccg aaccgaagcg caacgcctgc
     1081 tcgactcgtt gaacaacacg tcttccgcga atcgcagcag tggccccagt acgcgcagtg
     1141 ccgcccgagt gagtggcgcg ctggccgctg aacctgtggc cactaacacc ggtagaacgc
     1201 tgggcggcga ttcgagtggt gggaccgcga tcagagatgg aaggacaatc gaaacttccg
     1261 gttcattgcc tgcgctcgat gaagttctcg cacgatacgt tgaggcaatg ggaggcgcgg
     1321 acgcgatcaa ggccgtcacc agtcgtgtca caaagggaac agttgatgtg ccgggattaa
     1381 gccggggcgg gagtttcgag acgtctgagc aagcaccgaa caaggcgacg acggtgatac
     1441 aggcgcaccc gtttggaacg atcaagatcg gttacaacgg tcgcaacggt tggtcgaaag
     1501 gcgcggatcg tgttcaactg ataaaggacc gcacggagtt ggcggcgttg cagcgcgata
     1561 gcgatattta cggcgttgtg agattgaaga acaacttcgc aaaggtgacg ttggcgggaa
     1621 agagtgagat cggttatcgc gaagtttatg tactcgatct acagcctgcg gtcggatctg
     1681 tggagcgtct gtatctcgat gcaaaaactt atctgccggt gcgtatgaac acggtgcgca
     1741 aaattggggc ggtcgcggag gcggtggaga tctacctgga cgattggaaa gcggttgacg
     1801 gaatccaggt tccgttcagt gtcactcaac gctttcgcac actgacattg agctacacgg
     1861 tgaaagaggt ccgccacaac gtcccgctgg aaccgagcct gtttgaacca ccgattaaat
     1921 aaatctactc gcgatacaaa ttgtcgaagc gcatgctgga gggggtgaag cggagttcca
     1981 cggtgccggt tggaccattg cgctgtttgg cgataattaa ctccgcgatg ttcttccggt
     2041 cctcaggcat cgcatcgcgt tcttccggcg acttgtattg ctcctcgcga aagataaacg
     2101 ccaccagatc cgcatcctgc tccaacgcgc ccgactcgcg caggtccgcc aactgcggac
     2161 gatgatccga acgcgactcc ggcgcgcgcg aaagctgcga caacgcaacc aaaggcacgt
     2221 tcaactcctt cgccaaccct ttcaactcgc gcgagatctg cgacacttcc tgctgccgcg
     2281 actcgacccg tcttgccgaa cccgacatca actgcaagta gtcgacgacg atcaggtcga
     2341 gcttcttttg ttcggctgcg agccgccgcg ccttggcacg catttcgaga acactgatgc
     2401 cgggcgtgtc gtcgagaaaa atcttcgtgt cggcgagaac gccaagcgac
//
LOCUS       DASTEY010000360         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_73891, whole genome shotgun sequence.
ACCESSION   DASTEY010000360 DASTEY010000000
VERSION     DASTEY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73891"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFO72_06300"
     CDS             complement(<1..272)
                     /locus_tag="VFO72_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HET9478935.1"
                     /translation="MFRKLIATSSTWFPLPIRLALAAVMIAHGAQKVLGSFGGPGFKS
                     FISGNTPFSFMRPAWLWLAAAAFSEFVGGLLVGLGFLTRVAAFLIA"
     gene            complement(408..863)
                     /gene="trxA"
                     /locus_tag="VFO72_06305"
     CDS             complement(408..863)
                     /gene="trxA"
                     /locus_tag="VFO72_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9478936.1"
                     /translation="MKVVTCQKCEARNRVDESKLANSEAKCGRCGAKLNAQAGNGEAA
                     EPLILTDASFQQEVLQASGPPLLVDFWAPWCPPCRIVGPIVDQIAAESGGRYRVAKLN
                     VDDNPQTASRYQIASIPTMLIFKNGAVIDRIVGAQPKQAIVERLNQVTR"
     gene            941..2848
                     /locus_tag="VFO72_06310"
     CDS             941..2848
                     /locus_tag="VFO72_06310"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9478937.1"
                     /translation="MHKAPLCSGALLTFLFLFFTSLAAAQTVSSGAPEISFTVAMPRP
                     HTHLFEIDIAIKRGPSSTVPAHEQLVMPVWTPGSYLIREFQRHVQDFSAADAAGQPLK
                     WEKTNKTTWRVTTNGAREWHAKYRVYANELTVRTSELNSSHAFWNNANLLMYLDGYLK
                     SPATVRVVAPDVWKVATGLPGVPGQRNTFRAENFDVLYDSPFEASDFKSIVFNVKGVP
                     HRIVIDGEGNYDPERMRRDVQKIVETQVELMGGEIPYRDYTFFLHLRNDAGGGLEHAN
                     STALGYPRFGFRITRGDRATSAAPVTSGPPERDYRGFLSLVSHEFFHLWNVKRIRPDV
                     LGPFDYTQENYTKLLWVAEGITDYYADIALRRAGLITENEFLSATARAIQTLQNTPGR
                     MEQSVEESSFDTWIKFYRPDENSVNSQVSYYDKGAILGLLLDLEIRKRTSGAKSLDDV
                     MRYLYTEFFKQQRNYTPADFQKAAELAAGSSLEEFFAKYVRNTDELDYNSALAAAGLQ
                     LDTGITRTDGDKPVERVFFGADVVEQDERLMVRRVYAGSPAYEQGLNAGDQIVALDNM
                     RVTRDFFNARLAEKKPGDLINLTIFRFDNLSTLLIKLGGRADGTYRITTLPSQTEVQK
                     QIYRAWLGSRT"
     gene            2845..>3292
                     /locus_tag="VFO72_06315"
     CDS             2845..>3292
                     /locus_tag="VFO72_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET9478938.1"
                     /translation="MTHINFTVEDRVARITFARPPLNVLHIAMMREIAQALTESAQRE
                     IAAIVFDADKSCRAFSAGVAVEEHVEETIYQMLDEFHGIFRQLEQIAKPAIAVVDGAA
                     LGGGCELVAACDIVIASDRARFGQPEIKLGVFPPVAAILLPLVIGEK"
BASE COUNT          735 a          941 c          929 g          687 t
ORIGIN      
        1 cacgcgatca ggaaggcagc gacgcgagtc agaaatccga gtccgaccag taagccgccg
       61 acgaattccg agaatgcggc tgcggcaagc caaagccacg ccggacgcat gaaactgaaa
      121 ggtgtattgc cggagatgaa agacttgaaa cctggaccac caaagctgcc gagcaccttt
      181 tgcgcgccgt gcgcgatcat taccgccgcg agtgccagac gaattggcag tggaaaccac
      241 gttgaggagg tggcaatcag tttacgaaac attgcgattc cttcacgctg ctagtttgtg
      301 acagagccgc aagataatag cagtagtcag tggtcagtgg tcagtagtca gtggttcgtc
      361 cgtcgtgaac ccaccggtgc cactgaccac ggacaactga ccactgacta ccgagtaacc
      421 tgattcagtc gctcaacaat cgcctgcttt ggctgcgcgc cgacgatgcg atcgattacg
      481 gcgccgttct tgaagatcag cattgtggga atgctggcga tctgatatcg cgaagctgtt
      541 tgcggattgt cgtcgacgtt cagttttgcg actcgatagc ggccgcctga ctcagcagct
      601 atctggtcca cgattggacc aacgatgcgg cacggggggc accacggcgc ccagaaatcc
      661 accagcaacg gcggacccga tgcctgtagc acttcctgtt gaaacgaagc atccgtgaga
      721 atcaacggtt cggcagcctc gccattcccg gcctgcgcgt tcaatttagc gccgcagcga
      781 ccgcacttcg cctcgctgtt cgcgagttta ctttcatcca ctcgatttct ggcttcacac
      841 ttttgacacg taactacttt cattgccact cccctcactg cacgatattt aagtaggcag
      901 aagtcgcaaa agttttgcga aatctagtaa cattcccgac atgcacaagg ccccactctg
      961 cagcggcgcg ttgttgactt tcctgtttct tttcttcaca agtttggcag cggcgcaaac
     1021 agtatcttca ggcgcacccg aaatctcgtt cacggtcgcg atgccgcgcc cgcacacaca
     1081 cctgttcgag atcgacatcg cgatcaaacg cggtccatca agcaccgtgc ccgcacacga
     1141 acaactcgtg atgcccgtct ggactcccgg ttcctatctc atacgcgagt ttcagcggca
     1201 cgtgcaggac ttctccgcag cggacgcagc cggccagccg ctcaagtggg agaagacaaa
     1261 caaaacgacc tggcgcgtga cgaccaacgg cgcgcgcgag tggcacgcaa agtatcgagt
     1321 ttacgccaac gaactcacgg tgcggaccag cgagttaaac tccagtcatg ccttctggaa
     1381 caacgccaac ttgctgatgt atctcgacgg ctatctgaag agcccagcga ctgttcgcgt
     1441 cgtcgctcca gacgtgtgga aagtcgcgac gggccttccg ggcgtaccag gtcagcgaaa
     1501 tacgtttcgt gccgaaaact tcgacgtgct ttacgactcg ccgttcgaag ccagtgattt
     1561 caaatcgatc gtattcaacg tcaaaggcgt tcctcaccgc atcgtgatcg acggcgaggg
     1621 caactacgac cccgagcgca tgcgccgcga cgtgcagaag atcgtcgaga cgcaagtaga
     1681 gttgatgggc ggcgagatcc catatcgcga ttacacgttt tttcttcact tgcgcaacga
     1741 cgccggcggc ggactggaac acgcaaactc caccgcgctc ggttatccac gcttcggctt
     1801 tcgcatcact cgcggcgacc gggcgacttc ggccgcgccc gtcacgtccg gtccgcccga
     1861 gcgcgactac cgcggttttc tttcgctcgt ctctcacgaa ttctttcatc tttggaacgt
     1921 gaaacgcatt cgaccggacg tgcttggtcc attcgactac acgcaggaga actacacgaa
     1981 acttctctgg gtcgccgagg gtataactga ctactacgcc gacatcgcgc tgcgccgcgc
     2041 cggactgatc accgaaaacg agtttttgtc agccactgca cgcgcgatac agacgttgca
     2101 gaacacaccg gggcggatgg aacagtcggt cgaagagtcg agcttcgaca cctggatcaa
     2161 gttttatcgg ccggacgaga attcggtaaa ctcgcaggtg tcgtattacg acaagggcgc
     2221 tattttggga ttgttgcttg atttggaaat acgcaaacgc acgagcggcg ccaagtcgct
     2281 cgacgacgtg atgcggtatc tttacacgga gttttttaag cagcagcgaa actacacgcc
     2341 ggcggatttt cagaaggcgg ctgaactggc ggccggttcg agtttggaag agtttttcgc
     2401 aaagtacgtt cgcaacacag acgagctgga ttacaactcg gctctggcag cggctggtct
     2461 tcaactcgac accggcatca cgaggacaga tggagataag ccggttgaaa gagtcttttt
     2521 cggcgccgac gtagttgagc aggatgaacg tctgatggta cgacgcgtgt atgccggttc
     2581 tccggcttac gagcagggac tgaacgccgg cgatcagatc gtggcgctgg ataacatgcg
     2641 cgtcacgcgc gacttcttca acgcgcgtct cgccgaaaag aagccgggcg atctgatcaa
     2701 tctgacgatc ttccgttttg acaacttgag tacgttgctg atcaaactgg gcgggcgtgc
     2761 ggatggaact taccgcatta cgacattgcc gtcccaaacc gaagtgcaga aacagatcta
     2821 tcgagcgtgg ttaggatcgc ggacatgacg cacatcaatt tcacagttga agatcgcgtt
     2881 gcgcggatca cgttcgcacg cccgccccta aacgttttgc acatcgcgat gatgcgcgag
     2941 attgcgcagg cgttgaccga atcggcccaa cgcgagatcg cggcgatcgt cttcgatgcg
     3001 gacaaaagtt gtcgcgcgtt cagtgcgggc gtggcggtcg aagaacatgt ggaagagaca
     3061 atctatcaaa tgctcgatga gttccacggg atctttcgcc agctcgaaca gattgcgaag
     3121 ccggcgatcg cggtcgtcga tggtgcggca ctcggcggtg gctgtgagct ggtggccgcg
     3181 tgtgacatag ttatcgcgtc tgaccgggca cgttttggcc aaccggaaat taagctcggc
     3241 gtctttccgc cggtcgcggc gatcctgctg ccgctggtga ttggcgaaaa gc
//
LOCUS       DASTEY010000361         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1359615, whole genome shotgun sequence.
ACCESSION   DASTEY010000361 DASTEY010000000
VERSION     DASTEY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359615"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="VFO72_06320"
     CDS             <1..1465
                     /locus_tag="VFO72_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478939.1"
                     /translation="SKTAFGYPRRTLEDVPQGEYTVQALLHRYETFKRADGHTVKLPM
                     DRGEGQQWSRAPGNLYSTPKRMTIKGDLPINITLDKVIPPIEPPKDTKYIKHIKIQSG
                     RLTKFWGRPMHLGAHVLLPEGFDSHPEARYPLVVFHGHFPADFGGFREQPPDPNLKPD
                     YNQRFKLSGYNRIVQEHAHQFYKDWTGPNFPRFIIIEIQHANPYYDDSYAVNSANLGP
                     YGDAITYELIPEIEKRFRGIGQGWARFLYGGSTGGWEAMAAQVFYPDEYNGSWAACPD
                     PIDFRAYTVVNIYEHENAYYADSKWKRMPRPGKRNYLGELSATVEDMNYMELALGTNS
                     RSGGQWDIWQAVYSPVGSDGYPKPIWDKPTGKIDHSVAEYWRENYDLSYILKRDWAKI
                     GPKLAGKLNIYVGEADNYFLNNAVYLVEDFLKTTKDPAFGGEIDYEPRSEHCWNGDHT
                     RPNAISRLRYHQFFAPKIVERITKSAPPGADLKSWRY"
     gene            complement(1459..>2506)
                     /locus_tag="VFO72_06325"
     CDS             complement(1459..>2506)
                     /locus_tag="VFO72_06325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9478940.1"
                     /translation="RRFAPVRVTRDRVIREVVGLRPYRAGGFIVDAEKFGNKLLVHNY
                     GHGGGGVTLSWGTASLAVDLARDFLATSRARRRRFAVVGCGVSGLSTARLLQRRFQNG
                     PGTVTIYAKDLPPDTTSNVAGAWWSPTSVYEPDQVDDKFNEQFRRACQISNRAFQLLV
                     GAEYGVRWIDSFELLRSEMSLERELPGGDALYPARQVHRDPQHYFGFPFVRQYSTMLI
                     EPSIYLSALLRDFYIAGGRVVVKEFRNRREIARLPESVVFNCTGLGSRDLFDDPELVP
                     VRGQIEVLLPQPEIDYCYLSSGYMFPRRDGIVLGGTWDHGDWSLAPNSEQANSILETH
                     ATIMKNLIRVISVQ"
BASE COUNT          590 a          743 c          687 g          486 t
ORIGIN      
        1 cagcaaaacc gcgtttggtt atccgcggcg cacgctcgaa gacgtgcccc agggcgagta
       61 caccgtgcag gcgttgctgc atcgttatga aaccttcaaa cgcgcggatg gtcacacggt
      121 caagttgccg atggaccgag gcgaaggtca gcagtggagt cgcgcgccgg gaaatcttta
      181 cagcacaccc aagcgtatga cgatcaaggg cgatctgccg atcaatatca cgctcgacaa
      241 ggtcatcccg ccgatcgaac cgccgaaaga cacgaaatac atcaaacaca tcaaaatcca
      301 gagcgggcga ttgacgaagt tctggggacg cccgatgcat ctcggtgcgc acgtgctgct
      361 gcccgaaggt ttcgactcac atcccgaagc aagatatccg ctcgtcgtct tccatggtca
      421 cttcccggcc gacttcggtg gttttcgcga gcagccaccc gatccgaatc tgaagccgga
      481 ctacaaccag cgttttaaac tctccggcta caaccgtatc gttcaggagc acgcgcatca
      541 gttctataaa gactggactg gcccaaactt tccgcgcttt atcatcattg agattcagca
      601 cgccaacccg tattacgacg actcctacgc tgtcaactca gccaaccttg gtccatacgg
      661 cgacgccatc acttacgaat tgattcccga gatcgaaaag cgttttcgcg gcatcggtca
      721 agggtgggca cggtttctct acggcggttc caccggcggt tgggaagcta tggcggcgca
      781 ggtgttctat ccggatgagt acaacggcag ttgggccgca tgccccgatc cgatcgattt
      841 ccgcgcttac acggtcgtca atatttacga acacgagaac gcttactacg ccgattcaaa
      901 gtggaagcga atgccgcgtc cgggcaaacg aaactatctc ggcgagctgt cggctacggt
      961 cgaggacatg aactacatgg agttggcgct gggaacgaac agccgctcgg gtggccagtg
     1021 ggacatctgg caagcggtct attctcccgt tgggagcgac ggctatccga agcccatctg
     1081 ggacaagccg accggaaaga tcgaccattc cgtggccgag tactggcgcg agaattacga
     1141 tctcagctac atcctcaagc gtgattgggc caaaattggg ccaaagcttg ccggcaagct
     1201 caacatctat gtcggtgagg ctgacaacta ctttctcaac aacgcggtct atcttgttga
     1261 agactttctc aaaacaacca aggatcccgc cttcggcggt gagatagatt acgagccacg
     1321 ctctgagcat tgctggaacg gcgatcacac gcgcccaaac gcaatctcac ggttgcgcta
     1381 ccaccagttc tttgcaccga agatcgtgga gagaatcacg aagagcgcac cgccgggagc
     1441 ggatctcaag agttggcgct actgaacgct gattacacgg attaggtttt tcatgatcgt
     1501 ggcgtgggtt tcgaggatgc tgttggcctg ctcggagttt ggcgcgagtg accagtcgcc
     1561 gtggtcccag gtgcctccga gaacgattcc gtcgcgacgt ggaaacatgt aaccactact
     1621 gaggtagcag taatcgatct ccggttgcgg cagcaacact tctatttgac cgcgcacagg
     1681 caccagttcg ggatcgtcaa acaaatcgcg cgagcccagc ccggtacagt tgaaaacaac
     1741 tgactccggc agccgcgcga tctcgcgtcg attgcgaaac tccttcacga caactctgcc
     1801 accggcgatg tagaagtcgc gcaacaacgc gctgagatag atcgacggct cgatcagcat
     1861 ggtcgagtac tgccgcacga aaggaaaacc gaagtagtgt tgcggatcgc gatgaacctg
     1921 cctcgccgga tagagcgcgt cgccacccgg cagctctcgc tcgagcgaca tctcacttcg
     1981 caagagttcg aaagaatcga tccagcgcac gccgtactcc gctccaacca gcagttgaaa
     2041 agctcgattc gagatctgac acgcgcgccg gaattgttcg ttgaacttat cgtcgacctg
     2101 atccggttca tagacagacg ttggtgacca ccaggcgccg gcaacattcg aagtagtgtc
     2161 cggcggcagg tctttcgcat agatggtcac ggtgccggga ccgttttgaa aacgacgctg
     2221 taaaagacgc gctgttgaaa gaccactgac gccgcatcca acaaccgcaa agcgccgccg
     2281 tcgtgcgcga ctcgtcgcca agaagtctcg tgccagatcg actgccagcg acgccgtgcc
     2341 ccaggagagc gttacgccac cgccaccgtg accgtagtta tggaccagca gtttgttgcc
     2401 gaacttttcc gcgtcaacga tgaagccgcc ggctcggtat ggccgcaaac caaccacctc
     2461 acgaataacg cgatctcgcg tcaccctcac cggcgcaaag cggcgt
//
LOCUS       DASTEY010000362         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_139860, whole genome shotgun sequence.
ACCESSION   DASTEY010000362 DASTEY010000000
VERSION     DASTEY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139860"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1399)
                     /gene="moeB"
                     /locus_tag="VFO72_06330"
     CDS             complement(194..1399)
                     /gene="moeB"
                     /locus_tag="VFO72_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET9478941.1"
                     /translation="MIVPSIAGGAVAESEVAQLPELSNEEILRYSRHLIMPEVGMQGQ
                     RKLKAASVLMIGTGGLGAPVGMYLAAAGVGRIGVVDFDVVEESNLQRQIIHGTKDLGR
                     RKIESARDRLRDINPHIEIETHETRLTSHNAMQLFRNYDVVVDGTDNFPTRYLVNDAC
                     VLSGKPNVYGSIFRFEGQASVFWAEKGACYRCLYPEPPPPGLVPSCAEGGVLGVLPGI
                     VGAIQANETIKVILGAQDLLINRLLLFDAWSMKFREFKLRKDPKCPVCGEEPTVKELI
                     DYEQFCGITPQHAAPTEPKLEEITATELNARRLRGDRFQIIDVREPHEFDIAQLPGTK
                     LIPLNHVVTRMNEIDPEVETIVHCKGGVRSAKAIQALKRAGFSGRLINLKGGITAWSE
                     DVDPRVPKY"
     gene            complement(1385..1645)
                     /locus_tag="VFO72_06335"
                     /pseudo
     CDS             complement(1385..1645)
                     /locus_tag="VFO72_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
     gene            complement(1661..2092)
                     /locus_tag="VFO72_06340"
     CDS             complement(1661..2092)
                     /locus_tag="VFO72_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET9478942.1"
                     /translation="MITVSEDLLDEMRAHGVRDYPYECCGLLLGRNGAEGKFVSETYP
                     ISNAREESAKRNRFLIEPVELMRGERYAREHDLEVVGFYHSHPDSPAVPSQYDLEHAW
                     PTYSYIIVSTSPDQAGDLFSWEQEPDRSRFNREEIRITACG"
BASE COUNT          481 a          697 c          577 g          507 t
ORIGIN      
        1 atgcggcaac gatcgccacc caccttcgcc aatatgcatt cacaaagtca ttagccacaa
       61 aaaagacctg tggaggttta ctttgacgca ggtagggtta agagtgacgt gaatcgccgt
      121 tatcgtggga tgaattgagc cgcagattta cgcagattaa cgctgatctg cgtacatctg
      181 cgttaatctg cggctaatat ttcggcacgc gtggatcgac gtcttccgac cacgccgtga
      241 tcccgccttt caggttgatc aatctacccg aaaagcccgc gcgtttcagc gcctggatcg
      301 ctttcgcgct acgcacaccg cccttgcagt gaacgatcgt ctcgacctcg ggatcgatct
      361 cgttcatgcg agtcacgacg tgattcaacg gaatcaattt cgttcccggt agctgcgcga
      421 tatcaaactc gtgcggctcg cgcacgtcga tgatctgaaa ccgatcgcca cgcaatcgac
      481 gcgcattgag ctcagtcgca gtgatctctt ccagcttcgg ctcagtcggc gccgcatgct
      541 gcggtgtgat accgcaaaac tgctcgtaat cgatcagctc ttttacggtc ggctcttcac
      601 cgcacaccgg acacttcgga tccttgcgga gtttgaactc gcgaaacttc atcgaccacg
      661 catcgaaaag cagcaaacga ttgatcagca gatcttgagc gccaagaatg accttgatcg
      721 tttcgttcgc ctggatcgcg ccgacgatgc ccggcaacac gccgagcacg ccaccttcag
      781 cgcacgacgg cacgaggccc ggcggcggcg gctccggata caagcagcga tagcacgcgc
      841 ccttctcggc ccagaagaca ctcgcctgtc cttcaaacct gaatatggat ccgtaaacgt
      901 ttggcttacc actcaacaca cacgcatcgt tgacgagata acgtgtgggg aagttgtcag
      961 tgccgtcgac cacgacgtcg tagtttcgaa agagctgcat cgcgttgtgg ctggtgagac
     1021 gcgtctcgtg cgtctcgatc tcgatgtgcg gattgatatc gcgcaggcga tcgcgcgcgg
     1081 attcgatctt gcgccggccg agatcttttg tgccgtgaat gatctgtcgc tgcaggttcg
     1141 actcttccac cacgtcgaaa tcaaccaccc cgatgcggcc cacgccggcg gcggcaaggt
     1201 acattcccac aggcgcgccc agtccgcccg tgccgatcat caacacgctg gcagctttca
     1261 gctttcgctg gccctgcatg ccgacctccg gcatgatcag gtgacggctg taacgaagga
     1321 tctcttcgtt cgagagctcc ggcaactgcg ccacttccga ttcagccacc gcaccgccgg
     1381 cgatcgaagg cacaatcatg atcgtgtcgc cgtccttcac gggcgtctcc ggccctgacg
     1441 catggcggat atcttcgtcg ttgagataaa cgttgacgaa gttgcgaagc gcgttctgct
     1501 cattgaacag atggcgccgc aaggagatgt aagttgaagt aaggcgctcg agcgcttcgc
     1561 cggctgtttt tgcttctact acgatctgtg attgtccatc ggcgaactgc ctaagcggcg
     1621 ccggtatcgc aattgtgact ggcataattg acctctgttt tcagccgcag gctgttattc
     1681 ttatttcctc tcgattaaac cttgaacgat ccggctcctg ctcccaggaa aacagatcgc
     1741 cggcctggtc cgggctcgtc gaaacaatga tgtacgagta agtcggccac gcgtgctcga
     1801 gatcgtattg cgacggcaca gccggcgaat ccggatgcga atgataaaac ccgaccacct
     1861 ccagatcgtg ctcgcgcgcg tatcgctcac cacgcatcaa ctcgaccggc tcgatcaaaa
     1921 atcggtttcg cttcgcactc tcctcacgcg cgtttgagat cggatacgtc tcgctaacga
     1981 actttccttc cgctccattg cgcccaagca gcaatccgca acactcgtac ggataatctc
     2041 gcactccatg cgctctcatt tcatcaagca aatcttcgct aacagtgatc atccgtctgc
     2101 taatccgttt aatccgagtt cgatatccgc aatgatgtca tcgatcttct cgatcccaac
     2161 cgagatgcgt atcaacccct cgacaagtcc ccactctttt tttcgcgcag gcagcagatc
     2221 gcggtgcgac gccgtcgccg ggtgcaacac gctggtaaaa ac
//
LOCUS       DASTEY010000363         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_90544, whole genome shotgun sequence.
ACCESSION   DASTEY010000363 DASTEY010000000
VERSION     DASTEY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90544"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFO72_06345"
     CDS             complement(<1..512)
                     /locus_tag="VFO72_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HET9478943.1"
                     /translation="MRTTLTSPEKIHKLVNDAADNGFNTLIVQIRGRGDAYYKSKVEP
                     RAIELKEQPADFDPLALTLSEAHKRGLRVHGWINTNLLANLDALPSDPAHVYNKHPEW
                     LAVPKPVAAELFKMSPRDPAYRQKIVEWSKANRTELEGVYTSPANPKVKDHIYKIWMD
                     VLKRYRVDGL"
     gene            complement(621..>2129)
                     /locus_tag="VFO72_06350"
     CDS             complement(621..>2129)
                     /locus_tag="VFO72_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478944.1"
                     /translation="YEYVSAPREVEGRINYGFSADKDNWEPRLGFAWSPNFDDGFWHS
                     LFGNVGDSSFRGGYGIFHGRLFQSVFSQGGATVRFNPPNAFFFNQAGLASSIFNPVNL
                     ADPTNGFVFVPGPQTARHTLTLIDPGLEMPYTQQWNLTFERQMPFSSALRISYTGNRG
                     IGLLKYALNNLPLHDPVNGVLVPNHPNNPANLRGKVIRLAANFLCAGTTGTSAAPFTP
                     QCPVAVPIGEFEYSFRVPRTNERRPNGLHGTNVAVSNGSWSYYNALQLEWTKRLSSNL
                     NFQAAYTWSKAIDTTSEATFVGAGDSNQNGNNPRNSRGLSRFHTPHRFTIYGTYRLPF
                     FSGDRGLLGQAFGGWNFSAVVKLAKGTPFTVTTTALDLNFDGFGENRPVLLDTSILGN
                     SVNHPATSRNALPRAAFRTLTSADFNTPILGRNTFFVDGVKNVDLGINKLFRMPWEGH
                     TLTLRADMFNAFNHVQYGVPSAAITNTNFGAITGTAVQYLPRNIQVSLRYQY"
BASE COUNT          472 a          581 c          609 g          467 t
ORIGIN      
        1 tgcaaaccgt cgacgcgata acgtttcaac acgtccatcc agatcttgta gatatgatcc
       61 ttaactttcg gatttgccgg actcgtgtag acgccctcca gctcagtgcg attcgctttc
      121 gaccactcca cgatcttctg gcggtaagcc ggatcgcgcg gcgacatctt aaacagctca
      181 gcagcaacag gcttcggtac tgcgagccac tcgggatgtt tgttgtagac gtgcgccgga
      241 tcgctcggca gtgcgtcaag attcgcgagc aggttcgtgt tgatccaacc gtggactctc
      301 aagccacgct tgtgcgcttc cgagagggtc aatgcaagtg gatcgaagtc cgcgggttgt
      361 tccttcaact cgatcgcgcg tggttcaact ttggatttgt agtaagcgtc gccgcggcca
      421 cggatctgaa cgatcagtgt gttgaagccg ttgtccgcgg cgtcgtttac gagtttgtga
      481 atcttctccg gcgaagtcag agtcgtacgc acgacccaca acgcccgcac ctcattctgg
      541 cgcgccggct gtgccagtga aactgaacac aggcaaaaag aacagaagaa agtgacgagg
      601 agcttcaaca cagatccaaa tcaatactgg taacgcaacg acacctgaat gttgcgcggc
      661 aagtattgca ctgccgtgcc ggtgatcgcg ccgaagtttg tgtttgtaat tgccgcgctg
      721 ggcacaccgt actgaacgtg attgaaggca ttgaacatgt ctgcccgcaa cgtcagggtg
      781 tgaccttccc acggcatcct aaagagcttg ttaatgccaa ggtcgacgtt cttcactcca
      841 tcgacgaaga aagtgttacg accgaggatt ggcgtgttga agtcggcact tgtcagcgtg
      901 cgaaatgcgg ctcgcggcag ggcgttgcga gacgttgccg gatggttgac gctgttgccg
      961 agtatcgacg tgtcaagcag cacgggtctg ttttcaccaa atccgtcgaa gttcagatcg
     1021 agcgcggtag tggtgacagt gaacggcgtt cctttcgcaa gcttcacgac ggccgagaag
     1081 ttccaaccgc caaacgcctg gcccaacaga cctcgatcgc cgctaaagaa cggtaaccgg
     1141 tacgtgccgt agatagtgaa gcgatgcggc gtgtggaaac gcgagagccc gcgtgaattg
     1201 cgcgggttat ttccgttctg attactgtcg cccgctccga caaacgtcgc ctcagaagtg
     1261 gtatcgattg ccttgctcca cgtgtaagct gcctggaagt tcaaattgct gctcaaacgt
     1321 ttggtccatt ccagttgcag cgcgttgtaa taactccacg agccgttgga aacagccacg
     1381 tttgtgccgt gtagaccgtt tggccggcgc tcgttcgttc gcggcacgcg gaagctgtat
     1441 tcaaactcgc ctatcggcac ggctaccgga cactgcggcg tgaatggcgc tgcgctcgtt
     1501 cccgtcgtac cggcgcacag aaaattggcc gcaagccgaa ttactttgcc gcggaggttc
     1561 gccggattgt tcggatgatt gggaaccagc acgccgttaa ccggatcgtg cagcggcagg
     1621 ttgttgagcg cgtatttcaa caaaccgatg ccgcgattgc cggtgtaact aatccgaagc
     1681 gctgagctga acggcatctg gcgttcgaag gttaagttcc attgctgcgt gtaaggcatc
     1741 tccagtcccg gatcgatcaa ggtcagcgta tgccgagcag tctgtggccc aggaacaaag
     1801 acgaagccat tcgtcgggtc agcaaggtta accggattga agatcgacga ggcgaggcct
     1861 gcctggttga agaagaacgc gttcggtgga ttgaacctta ccgtcgcacc accttgcgaa
     1921 aagactgatt gaaacagccg tccgtgaaag attccgtagc cgccacggaa agacgagtcg
     1981 ccgacgttac cgaacaaact atgccagaag ccgtcgtcaa aattcgggct ccacgcaaaa
     2041 cctaagcgtg gttcccagtt gtccttgtca gcgctgaatc cgtagttgat acggccctcg
     2101 acttcccgcg gcgccgagac gtactcata
//
LOCUS       DASTEY010000364         5624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_980255, whole genome shotgun sequence.
ACCESSION   DASTEY010000364 DASTEY010000000
VERSION     DASTEY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980255"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFO72_06355"
     CDS             <1..585
                     /locus_tag="VFO72_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET9478945.1"
                     /translation="AFVVASSPLLIETLILNRVLFWILLFGQLGLVFYLAARVDKVAP
                     TTAAALFAAYSVSVGITSSVILLFYTGASVVSTFVVTAGMFGALALFGTFTRRSLAGV
                     GQFAFMGLIGLIIAMIVGLFWQNDALQFVISIVGVIVFTGLTAWDAQRLKEMALALPE
                     GRIGAYAVVGALSLYLDFINLFFFLLRFMGNRRD"
     gene            complement(858..1991)
                     /locus_tag="VFO72_06360"
     CDS             complement(858..1991)
                     /locus_tag="VFO72_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HET9478946.1"
                     /translation="MPRIVSFLPSATEIACALGLTDSIVGITHECDYPPAITTKPVVV
                     RNVLPIEQMSQSEIDRAVAERTREGQSLYQIDEELLRTLAPDLILTQNLCQVCAPSGN
                     EVSHVIKALPHAPQILWLTPQSLSEIFDNVGELGAATGRDAEAAALVNDCEARLKQLA
                     ERVDTAAGRAQQPGERLSEGVVADANRPRVFCMEWLDPVYASGHWVPELVKIAGGVDE
                     LGRERGDSVRVSWEEIAAWTPEVVVIMPCGFNLEQTMKQIWSVFGLKKSEAARQFFEL
                     PAVRTGRVYAVDANSYFARPGPRVVEGAELLARLIHPSLFAGEPIPAEFANAFQPVDV
                     ALLKGALEEDKDFYLERGAMVFTGSYLKRRGYCCGSGCRHCPY"
     gene            complement(2015..5050)
                     /locus_tag="VFO72_06365"
     CDS             complement(2015..5050)
                     /locus_tag="VFO72_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478947.1"
                     /translation="MLRLILMLAIVAIPVQAQMPDPAFFNGLRYRLVGPSRGGRVTTV
                     TGVPSQPKTFYMGVASGGLFRTIDNGATWTAITDGKVPLGSTGCVAVAESDPNVIYLG
                     TGSDGVRSNVSTGRGMYKSTDGGQTWKFIGLYNAGQIGAVRIHPKNPDIVWVSAVGDI
                     FKPNSERGVFKTTDGGQTWRKVLFLSDTVGAMDVELQPGNPNVVYAWMSRLERKPWTI
                     ISGSREGGFYKSTDGGEKFTKISAGLPNELIGKGNLAVTAAKPDRVYALIEAKPGGGF
                     YRSDDAGQTWSLINSQGSLIQRPFYYTTLGTDPSNADVVYAGAEGFFKSVDAGKTFTV
                     LRTPHVDSHDIWINPNNSEIMIQANDGGANVSTDGGRTWSTQMNQPTAEIYGVWVDNQ
                     FPYKLYGAQQDNTTLIISSQAEPFTVSDWRTGPGCETGPIMPHPLNPEIVYGSCKGQY
                     GVMNLKTGQQKNYWIGAQSLYGNPASDLIYRMQRVSPMAISPHDPEVLYYGSQYVHRT
                     RDKGVTWEKISPDLTARPECCQGISGEPITRDVTGEEFYSTLYAITESPLEKGVIWTG
                     ANDGPFHITRDNGKTWTNITPKDLPPGGRVQFIEASPHRKGSAYYAVYRWLLGDYQPY
                     IYRTDDYGKTWTRLTDGKNGIPADWPTRVVREDPDRQGLLYAGTEFGMFISFDNGAHW
                     QSFQLNLPNVPVTDIKVHRKDLIVSTQGRAFWILDNLSSLHQLTAQTKATEPILFKPR
                     DGYRTRVSPSNLGPVVEYLLPAVPAGAVVIEILDSKGAVLNSYNSEAPVLRTGRAGAG
                     ANMPGVGGAGPESQPADPDVARVRRGPAPPRVTKVAGLNRFAWDMRNQAGVTLPPGQY
                     QVRLKAGMRWLTQPLNVLIDPRVAADGVTLADLQEQFEHNMRIRELVTEVNQLIARVR
                     DAQNKLRDGSVSASSTGGDAFNADALNAVAAKLLTEPVRYGKPGLQAHITYLAGMTAN
                     VDQKIGRDAIERYRVLKKELETIRAEVDRILGSPVKSQI"
     gene            5082..>5624
                     /locus_tag="VFO72_06370"
     CDS             5082..>5624
                     /locus_tag="VFO72_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9478948.1"
                     /translation="MDRTLAQAIAEKYFGIQANAQELPSERDQNFLLAGREGEKFVLK
                     IANAREKREAIEAQNAMLNHLADRVAFCPRVVPATSGEQIATIENRLVRVLTYLPGKP
                     LATVRFQSPQLRRDLGRKLGQLNRALVEFDHQGLHRDLYWDLARGSQILCEFGPRIKT
                     DWLRELVLNYKADCWWTGLRK"
BASE COUNT         1089 a         1620 c         1644 g         1271 t
ORIGIN      
        1 gcgttcgtgg ttgcgtcctc tccgctttta attgagacgc taatcctcaa ccgagtgctg
       61 ttttggatcc tgctctttgg tcagctcggc ttggtgttct acctggcggc ccgggtcgac
      121 aaggtggcgc cgacaaccgc agccgcgctc tttgccgcct actcggtgtc ggtcggaatc
      181 acttcgtcgg tcatcctgtt gttctacacg ggcgcgtcgg tggtttccac ttttgtggtt
      241 acggcgggaa tgtttggtgc tttggcgctt ttcgggacgt ttacgcgaag gagtttggcg
      301 ggcgtcggcc agtttgcgtt catgggattg atcgggttga tcatcgcgat gatcgtgggc
      361 ctgttctggc aaaacgacgc attgcagttc gtgatctcga ttgtgggcgt gatcgtgttt
      421 actggtctga ccgcgtggga cgcacagcgt ttgaaggaga tggcccttgc gctgcctgaa
      481 ggacgtatcg gagcttacgc ggtcgtcggc gcgctatcgc tctacctcga ctttattaac
      541 ctcttcttct tcctattgcg cttcatgggc aaccgccgag actaaagctg cttaaaacct
      601 gggaccgcag gcgtctcgcc tgcacggtaa cgtaaaagct ctctgcgaac gatggaggcg
      661 agacgcctgc ggtcccaggt aagaaccgcc tcattactct tgcggtttct gccctctatc
      721 tcggtcatcg cgttattggt ttgggcaaag ttgtacagca cgctggcttt gagtgatcca
      781 tgctcgccgc cgagcaaatc tgggcagtgc ggtgaggatc tgggtgtgcg gcgcgcaaaa
      841 acgggcagtg gcggcatcta gtacgggcag tggcgacagc cgctgccgca gcagtagcct
      901 cggcgtttga gataactgcc ggtgaagacc attgcgccgc gttcgaggta gaagtctttg
      961 tcctcctcca gcgcaccttt caacagagca acgtcaaccg gttgaaacgc gtttgcaaac
     1021 tccgccggta ttggctcgcc ggcgaacagc gacgggtgaa tcaaccgcgc cagcagctct
     1081 gcgccttcaa cgacccgcgg gcccggtcgc gcgaagtacg agtttgcatc gactgcgtag
     1141 acgcgtcccg tccgcactgc cggtaactca aagaactgcc gcgccgcttc ggatttctta
     1201 agtccaaaga cactccagat ctgtttcatc gtctgctcga ggttgaaacc gcacggcata
     1261 attacaacca cttccggcgt ccacgccgcg atctcctccc acgacacacg cactgaatcg
     1321 ccgcgctccc tacccaactc atcgacgccg cccgcaattt tcaccagctc cggcacccaa
     1381 tggccactcg cataaaccgg atcgagccat tccatgcaga acacgcgcgg ccgattcgcg
     1441 tccgcaacga ctccttcgct caaacgctcg cccggctgtt gcgctctgcc ggctgccgta
     1501 tcgactcgtt cggccaactg tttaagccgc gcttcacaat cgttcacgag cgccgcagcc
     1561 tctgcgtctc tgccggtagc cgcgccgagt tcgcccacgt tgtcgaagat ctcgcttaac
     1621 gattgcggcg tcagccaaag aatctgcggc gcgtgcggca gcgctttgat cacgtgcgag
     1681 acttcgttcc ccgagggagc acaaacctga cacaggtttt gcgtcagtat cagatccggc
     1741 gcgagcgtgc gcagcagttc ttcatcgatc tggtaaagac tctgcccttc gcgcgtgcgc
     1801 tccgccactg cgcgatcgat ctcactctgg ctcatttgct caatcggcaa tacgttgcgt
     1861 acgacaaccg gcttagtcgt gatagccggc ggataatcac actcgtgcgt gataccgaca
     1921 atcgagtccg tgagtccaag agcgcatgct atttcagtgg ctgaaggaag aaacgaaaca
     1981 atccgtggca taagatcacg cttggtcctg gatttcagat ttgagacttc actggcgaac
     2041 cgagtattcg atctacttcg gcgcggatgg tttcgagttc ctttttcagc actcgatagc
     2101 gttcgatcgc atcgcggccg atcttctggt ccacgttggc ggtcatgccg gcgaggtaag
     2161 ttatgtgcgc ctggagacca ggtttgccat aacgcaccgg ttccgtgagc agttttgcgg
     2221 caacggcgtt caaagcgtct gcgttaaaag catcaccgcc ggtggaactt gctgaaacgg
     2281 acccatcgcg caacttgttc tgcgcgtcac gcaccctcgc gatgagctga ttgacctccg
     2341 tcaccagttc gcggatgcgc atgttgtgct caaactgctc ttgtagatcc gcgagggtca
     2401 caccgtcggc ggccacgcgc ggatcgatca gcacattgag aggctgagtc agccaccgca
     2461 tgccggcctt caaccggacc tggtattgac ccggtggaag cgtcacacca gcctgattgc
     2521 gcatgtccca ggcgaaacga ttcaaacccg cgactttggt gactcgcggc ggcgccgggc
     2581 cgcgtcgaac gcgcgcaacg tcgggatcag cgggttgaga ctcaggcccg gcgccgccaa
     2641 cacccggcat atttgcgccc gcgcctgcgc gaccagtacg gagcaccggt gcttcactgt
     2701 tgtaggaatt cagcaccgcc cccttgctgt cgaggatttc gatcaccacg gcccccgccg
     2761 gcacggcagg cagaagatat tcgacgactg gaccaaggtt cgagggactg acacgcgtgc
     2821 ggtatccatc ccgcggcttg aagaggattg gttctgttgc tttggtttgt gcggtgagct
     2881 gatgcaagga actgaggtta tcgagaatcc agaaagcccg gccctgcgtc gagacgatca
     2941 gatctttccg gtgcactttg atgtcggtta cgggcacgtt cggcaagttc aactgaaacg
     3001 actgccagtg cgcgccatta tcgaatgaga tgaacattcc gaattcagtg ccggcataga
     3061 gcagtccctg gcgatccgga tcttcgcgca cgacgcgcgt gggccaatca gcgggaatgc
     3121 cgttcttgcc gtctgtcaaa cgcgtccacg tcttgccgta atcgtccgtc cgataaatgt
     3181 atggctgata gtcgccgagc aaccagcgat acaccgcgta gtaagccgaa cccttacggt
     3241 gtggcgatgc ttcgatgaac tgcacccgtc caccgggcgg cagatctttc ggcgtgatgt
     3301 tcgtccaggt ctttccgttg tcgcgcgtga tatgaaaagg accgtcattc gctccggtcc
     3361 agatgacgcc tttctctagt ggcgactcgg tgatcgcata caacgtgctg taaaactcct
     3421 cacccgtaac gtcgcgcgta atcggttcgc cactgatgcc ctgacaacat tccgggcgcg
     3481 cggtcagatc cggcgagatt ttttcccacg taactccctt gtcgcgcgtg cggtgcacgt
     3541 actgcgaacc gtagtaaagc acctccggat cgtgcggtga gatggccatc ggcgacacgc
     3601 gttgcatgcg atagatcaga tcgctcgcgg gatttccgta gagcgactgc gcgccgatcc
     3661 aatagttctt ctgctgtccc gtcttcagat tcatcacgcc gtactggcct ttgcacgagc
     3721 cgtagacaat ttccggattg agcgggtgcg gcataattgg accagtttca caacctggac
     3781 cagtgcgcca gtcactaacg gtgaacggtt cagcctggct ggagattatc agcgtcgtgt
     3841 tgtcctgttg cgcgccgtaa agtttgtagg gaaactgatt gtcgacccaa acgccgtaga
     3901 tctccgcagt gggctgattc atctgcgttg accaggtgcg gccgccgtcc gtcgaaacgt
     3961 tggcgccgcc gtcgtttgcc tggatcatga tttcgctgtt gtttggattg atccagatgt
     4021 cgtgactgtc gacgtgtggc gtgcgcagga cggtgaacgt tttgcccgcg tcgacagact
     4081 tgaagaagcc ttcggcaccg gcgtaaacga cgtcggcatt cgaaggatcc gtgcccagcg
     4141 tcgtgtagta aaacgggcgc tggatcagcg aaccttgtga attgatcaac gaccacgttt
     4201 gtcccgcatc gtcggagcga taaaaaccac cgccgggttt ggcttcgatg agtgcgtaaa
     4261 cgcgatcggg cttcgccgcc gtgacggcca gattgccttt gccaattagt tcgttcggca
     4321 ggccggcgct gatctttgtg aatttctcgc cgccgtcggt gctcttgtag aaaccgccct
     4381 cgcgcgagcc actgatgatc gtccagggtt tacgttcgag ccgcgacatc cacgcataga
     4441 ccacgttagg attgccgggt tgcaactcga cgtccatcgc gccgacggtg tcagagagga
     4501 acagcacctt gcgccatgtc tgtccgccgt cagtcgtctt gaacacgccg cgctcgctgt
     4561 ttggcttgaa gatatcaccg actgcggaga cccagacgat gtccgggttc tttggatgaa
     4621 tacgcactgc tccgatctgc ccggcgttgt agagaccgat gaacttccac gtttggccac
     4681 cgtcggttga cttgtacatg ccgcggccgg tcgagacgtt gctgcgcacg ccgtcagaac
     4741 cggtaccgag atagatgacg tttggatccg attccgcaac cgcgacacag cccgtggaac
     4801 ctagtggaac ctttccgtca gtaatcgctg tccacgtcgc gccgttatca atggtgcgaa
     4861 acagtccgcc actcgcaaca cccatgtaga aggtcttcgg ttgtgaagga acacccgtta
     4921 cggttgtcac gcgtccgccg cgagatgggc caaccagtct atagcgtaag ccgttgaaaa
     4981 atgccgggtc gggcatttga gcctggactg gaatcgcgac gattgccagc attaagatta
     5041 accgcagcat gcaagacctc cgtttgagat ctatggaatc ggtggatcgt actctcgcac
     5101 aagctatagc cgagaagtat ttcggtattc aagcgaacgc gcaagagtta ccgagcgagc
     5161 gcgatcagaa tttcttgctc gccgggcgcg agggtgagaa gtttgtgctc aagattgcca
     5221 atgcgcgcga gaaaagagaa gcaattgaag cgcagaacgc aatgctgaat catctcgccg
     5281 accgggttgc gttctgtccg cgggttgtgc cggcgacgtc gggagagcag attgcgacta
     5341 ttgaaaaccg tctggttcga gtgttgacct acctgccggg aaagccgctg gcaaccgttc
     5401 ggtttcagtc gccgcagttg cgacgcgatt tggggcggaa gttggggcag ctcaaccggg
     5461 cgctggttga gttcgatcat cagggactgc atcgcgatct gtattgggac ttagcgcggg
     5521 gttcgcagat tctgtgcgag tttgggccac ggatcaagac ggattggttg cgtgagttgg
     5581 tgctgaacta caaagcggat tgttggtgga ctggtttgcg gaaa
//
LOCUS       DASTEY010000365         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_980259, whole genome shotgun sequence.
ACCESSION   DASTEY010000365 DASTEY010000000
VERSION     DASTEY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980259"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VFO72_06375"
     CDS             <1..1123
                     /locus_tag="VFO72_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478949.1"
                     /translation="ISLVAETSSGIEPNFSWAYVRKDTLGTRTYVHTLAAQALGIDVD
                     QCDQESIDAAAAYVVEHERELPSHFISAMNISAEQHVHVLAAAQRNVDNSVSKTCNGA
                     IDDTVESVDALYRLGRQLGCKAVSYYRDGSRDNQVLTSMKAEEPKGEIACNPEAVVEP
                     VAEATAGTAAAGTEAVSQAEAVAADDASARQEAIAREHSDTVPVPAVTAIRIERPREL
                     RGATWQIPFDGQNLYVTVNHDGKMIQEVFATGPISGGVGLLASKMLRGGFDAAEVAYS
                     LNKVTGTHAVWFNERLLTSPEQAVAECIMITVRRVQDLPDSARAVSKHTSQLHPQQQR
                     PVTGAIMSNMIGICPECHGQLEHASGCDFCRDCGYSKCK"
     gene            complement(1335..>2602)
                     /locus_tag="VFO72_06380"
     CDS             complement(1335..>2602)
                     /locus_tag="VFO72_06380"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HET9478950.1"
                     /translation="LSLCSLSFRESLAQPQLRVNGDRITKNIQALAEFGKNPQGGVSR
                     VAYSDADKQGREYVLGLLRDAKLNVTIDAAGNLIGRRAGSANGLKPLLIGSHIDSVPE
                     GGNYDGVVGSMGAIEVAQTLAENNVSLRHPLEVVIFQNEEGGLIGSRAMDGELTEREL
                     DLVSRSGKTIREGIKFIGGDPTKLAGVRRNKGDIAAYLELHIEQGAYLDTEKIKIGVV
                     EGIVGINWWDVTIEGFANHAGTTAMNNRQDALLAAAKFIEAVNRIVTSVPGRQVGTVG
                     RINALPGAPNVIPGKVVLSLELRDLDAAKINMLYEKIRAEAEQIAAASKTKFDFKEIN
                     VNIPAPTDVRIRSFISEAARELGLSTKLMPSGAGHDAQDMARLGPVGMIFVPSVGGIS
                     HSPREFSHPEDIANGANVLLHTLLKLDRA"
BASE COUNT          515 a          741 c          754 g          592 t
ORIGIN      
        1 tatctcgctg gtggcggaaa cgtcgtcagg cattgagccc aacttctcat gggcttacgt
       61 gcgcaaggac actctcggca cgcgcactta cgtgcatact ctcgcggcac aggcgctcgg
      121 tattgatgtg gaccagtgcg atcaggaatc gatagatgcg gctgcggctt acgtcgtgga
      181 acacgagcgt gagttgccgt ctcattttat ctcggcgatg aacataagcg ctgagcagca
      241 cgttcatgtg ctcgcggctg ctcagcgcaa cgtcgataac agcgtgtcga agacgtgcaa
      301 tggcgcgatt gacgacacgg tcgagtcggt cgacgccttg tatcgccttg gtcgtcagct
      361 tggttgcaag gcggttagtt actatcgtga tggatcgcgt gacaatcagg tgctcacgtc
      421 gatgaaagcg gaagagccaa agggtgagat tgcgtgcaat ccggaagcag tcgtcgagcc
      481 ggttgcggaa gctactgctg ggacggcggc cgcggggact gaagctgtca gtcaggccga
      541 ggcggttgcg gctgacgatg cgtcagctcg acaggaggcg attgctcgtg agcactccga
      601 taccgttccc gtgccggctg tgacggcgat ccgtatcgag cgtccgcgtg aactgcgcgg
      661 agcaacgtgg cagattccgt tcgacggtca gaatctttac gtgactgtaa atcacgacgg
      721 caagatgatc caggaggtgt ttgcgactgg tccgatcagt ggcggtgtgg gtctgttggc
      781 gtcgaagatg ttgcgtggtg gtttcgatgc ggcggaagtc gcgtacagtc tcaacaaagt
      841 caccggcacg catgctgttt ggttcaacga acgtctctta acatcacctg agcaagccgt
      901 agcagagtgc atcatgatca ccgtgcgccg cgtccaggat ctccctgact cggcgcgtgc
      961 ggttagtaag cacacctctc aactccaccc gcaacaacaa cgccccgtca ccggcgccat
     1021 catgtcgaac atgatcggca tctgccccga atgccacggc caattggagc acgcaagtgg
     1081 ctgcgatttt tgccgggact gcggatatag caagtgcaag tgagtttcgc cctctgatcg
     1141 tgcgttcgcg ttgacgccgg tgatgtcgcc tttgctgttg gcgtcaccgc aacagacccg
     1201 acgacgttta cgctgatttt gttgtttctg attggcgttg ccgcaatggc gagctacatc
     1261 ccggctcgtt gcgcgacaaa ggtcgatccg ataaccgctt tacgctacga gcagagaccg
     1321 ggaggttggc ggcttcatgc tcggtcgagc ttcaacaacg tgtgcagcaa gacgttcgcg
     1381 ccgtttgcga tgtcttcagg atgtgagaac tcgcgtggtg agtggctaat gccgcccacg
     1441 ctgggaacaa aaatcattcc gactggtcca agccgggcca tgtcctgagc atcatgaccc
     1501 gcgccggatg gcatcagttt cgttgacaga ccgagttcgc gcgcggcttc gcttatgaaa
     1561 gaacgaatgc ggacgtcagt cggtgcgggg atgttgacgt tgatctcttt aaagtcgaac
     1621 ttcgtcttgc tggccgccgc aatctgttca gcttccgcgc gaatcttctc gtaaagcatg
     1681 ttgatcttcg ctgcgtccag gtcgcgcagt tcgagactca gcacgacctt gccgggaatg
     1741 acgttcggcg cgcccggtaa cgcgttaatc ctgcccacgg tgcccacctg ccgtcctggc
     1801 acgctcgtga cgatgcgatt cacagcctcg ataaacttcg cggccgccag caacgcatcc
     1861 tggcgattat tcatcgcggt cgtgccggca tgattggcaa acccttcgat cgtgacgtcc
     1921 caccaattaa tgcccacgat cccttcgaca acgccgatct tgatcttttc agtgtcgaga
     1981 tacgcgcctt gttcgatatg caactcgaga taggccgcaa tgtcaccttt gttacgacga
     2041 acgccggcaa gttttgtggg atctccaccg atgaacttaa tgccctcacg aatcgttttg
     2101 ccgctgcggc tgacgagatc gagttcgcgt tcggtaagtt cgccatccat cgcgcggctt
     2161 ccgattagcc caccttcttc gttctgaaag atcaccacct cgagtggatg gcgcagtgag
     2221 acgttgttct cggcaagtgt ttgtgccacc tcaatcgcgc ccatcgagcc gacaacgccg
     2281 tcgtagtttc cgccttcggg aacgctatcg atatgcgatc caatgagtaa cggcttcagc
     2341 ccgtttgcac tcccagcccg gcgtccgatg agatttccag cggcgtcgat cgttacattc
     2401 agttttgcgt cgcgaagcag gcccagaaca tactctcgcc cctgcttgtc ggcatcgcta
     2461 taagcaacgc gactgacacc gccttgcggg tttttgccaa attccgccag tgcctggatg
     2521 tttttcgtga tccggtcgcc gttgacgcgc aactgtggtt gcgccaggga ttcacgaaac
     2581 gacaacgaac acagactcaa ga
//
LOCUS       DASTEY010000366         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_898435, whole genome shotgun sequence.
ACCESSION   DASTEY010000366 DASTEY010000000
VERSION     DASTEY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898435"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFO72_06385"
     CDS             <1..975
                     /locus_tag="VFO72_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET9478951.1"
                     /translation="DFHYRDFRKYPDGQTLWVTTTDANAEEKAGAARPHFGGGERVYN
                     VIDSRQSYPQEIVARGVAAVVPEVGEDASVHFSYEMVALSPAACEELGIALSEEDRAR
                     PYIEMSGRKGLGVKADDLIDQLEAGALVEVEKRNPSLSNDERIATAHEVAVAALRYFL
                     LKFTRNTVIAFDFKEALSFEGETGPYCQYAAVRTNSIFRKLGDETLRHADSLLERVVG
                     DSALQNRVGEVLSGESGTEIWSLLMLTQQLDEVIEQCRKSAEPANLAKYTFSVARSFS
                     RFYQRDENRIIGEKDEARRAVLIAVTKIVRAQLTAALSILGIHVPEQM"
     gene            1063..2070
                     /gene="aroF"
                     /locus_tag="VFO72_06390"
     CDS             1063..2070
                     /gene="aroF"
                     /locus_tag="VFO72_06390"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9478952.1"
                     /translation="MKSDATDSQVDAVLRVIEELGFKGHPMPGATRTAIGITGNQGAV
                     DPSHFENLAGVAEAIRVSKPYKLITLDLRPEKTVVRVGDAPIGGDELAIIAGPCAIES
                     RAQAFAIAEAVRRSGARFFRGGAYKPRTSPYAFQGLGEEGLRILADVREAYGLKIVTE
                     ALDEAGVDLVERYGDMIQIGARNMQNFSLLRRAGKAKLPVLLKRGLSATLDEWLLAAE
                     YVMAEGNYHIVLCERGIRTFAQHTRNTMDLAAIPAVRRISHLPVIVDPSHGTGKNFMV
                     TPLARAGVAVGADGLIVEVHDQPEHALSDGAQALTLEQYEELVTEVRSIHEVIAPAAV
                     S"
     gene            complement(2067..>2336)
                     /locus_tag="VFO72_06395"
     CDS             complement(2067..>2336)
                     /locus_tag="VFO72_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478953.1"
                     /translation="AVGVDISFPRTKESLIEKARQADLVIVDLHGQRFDPLTLPGDLG
                     TTRLLGFFSHVEVDLQQQALSAGYDRVVPRSVFARDLAQILTDTD"
BASE COUNT          539 a          646 c          689 g          462 t
ORIGIN      
        1 gatttccact accgagactt tcgcaagtat ccggacggcc aaaccctctg ggtcactacc
       61 acggatgcga acgccgaaga gaaagcagga gcggcacggc cacacttcgg cggcggcgaa
      121 agagtctata acgtgatcga ctcgcggcag tcgtacccgc aggagattgt ggctcgcggc
      181 gtcgccgcag tagtgccgga agtgggcgaa gatgcaagtg tgcacttcag ctacgaaatg
      241 gtggcgctct ccccggccgc gtgcgaagaa ctcggaattg cgttgagcga agaggatcgc
      301 gcgcgaccct acatcgagat gagcggccgc aaaggtctcg gcgtcaaggc cgacgatcta
      361 atcgaccaac tcgaagccgg cgcactcgtc gaggtcgaga aaagaaatcc gtcgctctcg
      421 aacgacgaac ggatagcgac agctcacgag gtcgcggtgg cggcgttgcg gtatttcctg
      481 ctgaagttta cacgcaacac cgttattgcc tttgacttca aagaagcgct ttcgttcgag
      541 ggcgagactg gtccatactg ccagtacgcc gctgtgcgaa caaactccat cttccgcaag
      601 ctcggtgatg aaactcttcg ccacgcggac tcgttgttgg aaagggtggt tggcgacagc
      661 gccttgcaga atcgcgtagg agaagtgctg tctggggaga gtgggacgga gatctggtcg
      721 cttctgatgt tgacgcagca gttggatgaa gtgatcgagc agtgtcgcaa atcagccgaa
      781 cccgcaaacc tcgcaaaata caccttttcc gttgcgcgaa gcttcagccg cttttaccaa
      841 cgggacgaaa atcgcatcat tggtgagaaa gacgaggctc gccgcgccgt gctgatcgcc
      901 gtcacgaaga tagtgcgcgc ccaactgacg gcggcgcttt cgatcctggg aatccacgtg
      961 cccgagcaga tgtaggacag gcattcctgc ctgtcttttt aacaaaaacg acagacaaga
     1021 atgtctgtcc tacggtagga taaaagcaac atgttaatcg taatgaaatc cgacgccacc
     1081 gacagccagg ttgacgctgt cctgcgcgtg atcgaagagc tgggcttcaa aggccatccg
     1141 atgccgggcg cgacgcgcac cgcgatcgga attactggca atcagggagc ggtcgatccg
     1201 tcgcacttcg aaaatctcgc gggcgtcgcg gaagcaattc gcgttagtaa accttacaaa
     1261 ctgatcacgc tggacctgcg tccggaaaaa actgtcgtgc gcgtcggtga cgcacccatt
     1321 ggcggcgacg agttggcgat catcgccggt ccctgtgcaa tcgaaagccg cgcgcaggca
     1381 tttgcgatcg ccgaagccgt tcgccgcagc ggcgcacgtt ttttccgcgg cggcgcttac
     1441 aaaccgagaa cttccccgta tgcatttcaa gggttgggtg aagaaggact cagaatactc
     1501 gccgacgtcc gtgaagccta cgggctaaag attgttaccg aagcgctgga tgaggcgggc
     1561 gtggatctgg tcgaacgtta cggcgacatg attcagatcg gcgcgcgaaa catgcagaac
     1621 ttctcgttgt tgcgccgtgc cggcaaagcg aagctgcccg ttttattgaa gcgcggattg
     1681 tcggcgaccc ttgatgagtg gctactcgcg gccgagtacg tgatggccga aggcaactat
     1741 cacatcgtgc tttgcgaacg aggcattcgc acctttgcgc agcacacgcg caacacgatg
     1801 gatctcgcag cgattccggc agtcaggcgt atctcgcact tgccggtcat cgtcgatcca
     1861 tcccacggaa ccggaaaaaa cttcatggtt acaccgctag cgcgcgcagg agtcgcagtc
     1921 ggggcggatg gattgattgt cgaagttcac gaccaaccgg aacatgcgct atccgacgga
     1981 gcacaggcgt tgacgctcga gcagtatgag gagttggtga ccgaggtgcg ttcaattcac
     2041 gaggtgatcg cacccgctgc ggttagttaa tccgtgtcag tcagtatctg cgccagatct
     2101 cgggcgaata ccgatcgcgg tacaactcga tcgtaaccgg ccgagagcgc ttgttgttgc
     2161 aggtcaactt cgacatgtga aaagaaaccg agtaagcgcg ttgtgcccaa gtctcccggc
     2221 aaagttaagg gatcaaatct ctgaccatgt aaatcgacga ttactaaatc cgcttgtcgc
     2281 gccttctcga tcaacgattc cttcgttcgc ggaaaactga tatctacccc caccgc
//
LOCUS       DASTEY010000367         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1376040, whole genome shotgun sequence.
ACCESSION   DASTEY010000367 DASTEY010000000
VERSION     DASTEY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376040"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFO72_06400"
     CDS             <1..331
                     /locus_tag="VFO72_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9478954.1"
                     /translation="GMLASATIGGEVGLYEPVHGSAPDIAGQGIANPCGAIASAAMML
                     RHSAHLEQEACDIELAIQHVLDAGYRTPDIATGTGGYLATTSEIGELVCQAVTEIADM
                     RHAYHAV"
     gene            458..1861
                     /gene="leuC"
                     /locus_tag="VFO72_06405"
     CDS             458..1861
                     /gene="leuC"
                     /locus_tag="VFO72_06405"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET9478955.1"
                     /translation="MPTLLDKLWQAHVVREAQGEPTLLYIDLHLIHEVTSPQAFAGLR
                     QAGRTVRRPDLTFGTIDHNVPTTDRSLPIADAIAATQIETLRRNCEEFGVQLFDIHSP
                     EQGIVHVIGPELGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLPQN
                     KPRSMKVEVSGALPEGVSAKDLALGIIGKIGTDGATGHVIEYAGEAVRSLSMEGRMTL
                     CNMSIEAGARAGMIAPDETTFAYLKGRRFAPAGERWEKALSYWRTLVSDETAVFDATV
                     NIDASQLEPFVTWGTSPGMVVPVTSCVPDPAEARTATDRQAAERALEYMALAPRTSIR
                     EIAIDRAFIGSCTNARLEDLRAAARVVKGYRIRPSVSAIVVPGSQEVKRAAEREGLDR
                     IFSEAGFEWREAGCSMCLGMNADILQPGERCASTSNRNFEGRQGRGGRTHLVSPMMAA
                     AAAIKGHFTDVREWEFK"
     gene            1861..2436
                     /gene="leuD"
                     /locus_tag="VFO72_06410"
     CDS             1861..2436
                     /gene="leuD"
                     /locus_tag="VFO72_06410"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9478956.1"
                     /translation="MKAFRRHTGLVAPLNRANVDTDQIIPKQFLKRIERTGFGQFLFY
                     DWRYRPDGQLNPSFVLNEPRYQGASILVADKNFGCGSSREHAPWALSEFGFRVIIAPS
                     FADIFANNCLKNGMLPIAIPVDEIMTRAQELEGYRLTVDLETQMITDENGLSVPFVVE
                     SFQRYCLLEGLDDIGLTLRHEEAIKAYEASR"
     gene            complement(2411..>2604)
                     /locus_tag="VFO72_06415"
     CDS             complement(2411..>2604)
                     /locus_tag="VFO72_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478957.1"
                     /translation="ATVNTLPQQGEDGGQYGVSVTDSSGSLGKYRYLLFDCYVTELYD
                     NWGNTFYSEIDVISRLRRP"
BASE COUNT          559 a          698 c          787 g          560 t
ORIGIN      
        1 tgggatgctt gcgtcggcga cgatcggcgg cgaagttgga ctctacgaac cggtgcacgg
       61 gtccgcgcct gacattgcgg gccagggcat cgcgaatcct tgcggcgcca tcgcgtcagc
      121 ggcgatgatg ttgcgccact cggcgcatct cgagcaggaa gcgtgtgata tcgaactcgc
      181 gattcaacac gtgctcgacg ccggctatcg tactcccgac atcgcaaccg ggaccggcgg
      241 ttatctggcc accacgagcg agattggtga gttggtctgc caggctgtga ccgaaatcgc
      301 cgatatgcgc cacgcgtatc acgctgttta gccctgggat cgcaggcgtc tcgcctgctg
      361 tgtttcgcag gtagttcggt gcgacgaatg caggcgagac gcctgcggtc ccagagtaca
      421 cgggtcctat ttacaaattg agttttcaag gaaaccggtg cctacgctgc tcgacaaact
      481 ctggcaagct cacgttgttc gtgaagcgca gggcgaaccc acgcttctct acatcgatct
      541 gcacctcata cacgaggtga cgtctcccca agcttttgct ggtctgcggc aagcaggccg
      601 aactgttcgc cgtcccgatc tgacgttcgg caccatagat cacaacgttc caactacaga
      661 tcgtagtctg ccaatcgccg atgcgattgc cgccacgcag atcgagacat tgcgccgcaa
      721 ttgcgaagag tttggcgtgc agttgttcga cattcactcg ccagaacaag gcatcgtgca
      781 tgtcatcggt ccggaacttg gtctgacgca accaggcatg accatagtct gcggcgatag
      841 ccacacgagc acgcacggag cgttcggcgc gctcgcgttt ggcatcggca cgagtgaagt
      901 cgaacacgtg cttgcgacgc agtgtctgcc gcaaaacaaa ccgcgctcga tgaaagtcga
      961 agtttcggga gcgcttccgg aaggtgtgtc tgcaaaagat ctcgcgcttg gaatcatcgg
     1021 gaagatcggc actgacggcg caacaggcca cgtgattgag tacgccggtg aagcggtgcg
     1081 ttcactttcg atggaaggcc ggatgacgct ctgcaacatg agcatcgagg ccggcgcgcg
     1141 cgccggaatg attgcgccag atgaaacgac atttgcatat ttgaaagggc gccggtttgc
     1201 tcctgcgggc gagcgttggg aaaaggcgtt gagctactgg cgcactctcg taagcgacga
     1261 gacagccgtt tttgacgcca cggtgaatat cgatgcgtcg caactcgaac cgtttgtgac
     1321 gtggggcacg agtccgggga tggttgtacc ggtgacgagt tgcgtgcctg atccggctga
     1381 agcgaggacg gcaaccgatc gccaggcggc agagcgtgcg ctcgaataca tggcgcttgc
     1441 gccgcgcacg agtattcggg agatcgcgat tgatcgtgct ttcattggtt cgtgtactaa
     1501 cgcgcggctt gaagatctgc gtgcagccgc gcgcgtcgtg aaaggttacc ggattagacc
     1561 gtcagtgagc gcgatagttg tgccgggctc gcaggaggtg aagcgtgccg cggaacgcga
     1621 aggtctcgat cggatcttca gtgaagcggg atttgaatgg cgcgaagcgg gctgctcgat
     1681 gtgtttggga atgaacgccg acatcctgca accaggtgag cgttgtgctt cgacgagcaa
     1741 tcgaaacttc gaagggcgtc aggggcgtgg cgggcgcacg catctcgtga gtccgatgat
     1801 ggccgcggcg gcggcgatca agggccactt cacggacgta cgcgagtggg agttcaagtg
     1861 atgaaagcgt ttcgccggca cacgggtctc gtcgcgccac ttaatcgcgc gaacgttgac
     1921 acggaccaga tcattccgaa gcagttttta aaacggatcg agcgcactgg ttttggtcag
     1981 tttctctttt atgactggcg ttatcgtcca gacggacaat tgaatccgtc gtttgtccta
     2041 aacgagccga gatatcaggg tgcatcgatt ctcgttgcgg acaaaaactt cgggtgtggt
     2101 tcgtcgcgtg agcatgcgcc ttgggcactc agcgagtttg gctttcgcgt tattatcgcg
     2161 ccgtcgtttg cggatatctt tgctaacaac tgtcttaaaa atggaatgct gccaatcgct
     2221 atccccgtcg acgaaatcat gacgcgagcg caagaactcg aaggttacag attaaccgtc
     2281 gatcttgaaa cacagatgat cacagatgaa aacggattgt ccgttccatt cgtggtggaa
     2341 agttttcaac gttattgcct gctggaaggc ctggacgaca tcggactcac tctcagacac
     2401 gaggaagcta ttaaggccta cgaagcctcg agatgacatc gatttcgctg tagaacgtgt
     2461 tgccccagtt gtcgtacaac tcagtcacat aacaatcgaa cagcaagtaa cgatacttgc
     2521 ctaagctgcc gctcgagtcc gtcacgctca cgccatactg gccgccatct tcaccttgtt
     2581 gaggcaacgt atttacagtc gcaa
//
LOCUS       DASTEY010000368         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1507967, whole genome shotgun sequence.
ACCESSION   DASTEY010000368 DASTEY010000000
VERSION     DASTEY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507967"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFO72_06420"
     CDS             complement(<1..678)
                     /locus_tag="VFO72_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9478958.1"
                     /translation="MSQLTKTRASEILQTLRERKVLVLGDVMLDEFVWGDVTRISPEG
                     PVPVVDVRRESVHLGGAANVLANLIALGAHGWVVGVVGNDSAGERLREELRQLNSGGD
                     GAVIVDETRRSTTKTRIVAHSQLVVRADRESRAPVSGKVEEQIVSRLKEKLDEADAFV
                     VSDYDKGVITPGILREVLPFAYERVPVLVDPKLRNFSSYRPATLITPNHHEALRMSDT
                     EDHSDDGS"
     gene            complement(666..1796)
                     /locus_tag="VFO72_06425"
     CDS             complement(666..1796)
                     /locus_tag="VFO72_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET9478959.1"
                     /translation="MNSLRDIVESSPQFGAVKTEPAFREDAPQPLAPPRWNWESVQRV
                     LIVRLRSIGDTVLATPSLFALKRFLPQARVDILVEEWVAPLLTDHPHVDNVVVLERGG
                     LVARTRVARELRAARYDVVYNLHGGTTATFLTRATGARHRVGFKTYQYAKLHTQLAPS
                     PLLLWGQQKTHSVEQQLALLGWTGVPVSDRPRTRLGISAEAVATVNQHLAAAELADRK
                     IALIHPAAAFETKRWASENFAHVAEFLADRGFAPVAIAAPHEAQVISDLLTAASVKIV
                     ALDLSLPEVIALASRSQLFVGNDSGIAHIAAAVGTPSVVVFGSSNVAHWRPWNRAAAH
                     VVIEEMPCQPCHGYFCEKFPQPECILRVPVDRVIAAIEKCLN"
     gene            complement(1801..1971)
                     /locus_tag="VFO72_06430"
     CDS             complement(1801..1971)
                     /locus_tag="VFO72_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET9478960.1"
                     /translation="MAVSQELLEILACPACKAKVELKPDGNALKCVECKRVYPIRDDI
                     PVMLIDEATIEE"
     gene            2088..>2723
                     /locus_tag="VFO72_06435"
     CDS             2088..>2723
                     /locus_tag="VFO72_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478961.1"
                     /translation="MSLEFALQYACPLRASYSERHLREWGRLSSLHAKLTTGDAVTPS
                     IDKLEWVEKASPEIERMQRATTICAHCPACLPLDIAGDGESVGCLGRINYPIEAVFEK
                     FLADRVQLSLDTLDKEDQPRLLRILVDPESPFDGEATKELRRVVTEQGLRFFELRVPI
                     VLTREAARLSTDNVFDLLAGFRSEDTQQTTYTRELPFEAAADYYDFLDLILR"
BASE COUNT          568 a          796 c          788 g          571 t
ORIGIN      
        1 cgagccgtcg tcggaatgat cctccgtgtc gctcatccgc aaagcttcgt gatgattcgg
       61 tgtaatcagc gtggccgggc gatacgaact gaagttgcgc aacttcggat cgaccagaac
      121 cggaacacgc tcgtacgcaa acggcagcac ctcgcgcagg atccccggcg tgatcacacc
      181 tttgtcgtag tcggaaacga caaacgcatc cgcctcatcg agtttctctt tcaatcgcga
      241 aacgatctgt tcttcgactt tcccgctgac cggcgcgcgc gattcacgat ccgctcgcac
      301 caccaactga ctgtgcgcga cgatgcgcgt cttcgtagtt gaacgccgtg tctcatcaac
      361 aatgactgcg ccgtcgccgc cggagttcaa ttgtcgtaac tcctcacgca gtcgttcgcc
      421 agccgaatcg tttccgacga caccgacaac ccaaccgtga gcgccgagcg cgatcaggtt
      481 ggcgagcacg tttgccgcgc cgccaagatg gaccgactcg cgtcgcacat ccacaaccgg
      541 cacgggccct tcgggagaga ttcgcgtgac gtcgccccag acaaactcgt cgagcatcac
      601 gtcaccaagc acgagcactt tacgctcgcg aagcgtttgc aggatttcgg aggcgcgagt
      661 ttttgttagt tgagacattt ctcgatcgcg gcgatcactc ggtcgaccgg cacgcgcaga
      721 atacactccg gctgcggaaa cttctcgcaa aaatagccgt gacacggttg gcagggcatc
      781 tcttcaatta ctacgtgtgc ggcggcgcgg ttccacgggc gccagtgggc cacgtttgac
      841 gaaccaaaaa caactaccga aggcgtaccg accgccgcgg cgatatgcgc aatgccactg
      901 tcgttgccga cgaatagttg tgaacgcgac gccaaagcga tgacttccgg tagcgacaga
      961 tccagcgcaa cgatcttcac ggatgctgcg gtaagcagat cgctaatcac ctgcgcctcg
     1021 tgcggcgcag cgatcgcaac gggcgcaaaa ccccggtcag ccaaaaactc cgcgacgtgc
     1081 gcaaagttct cgctggccca acgcttcgtc tcaaacgcgg ccgctggatg aatcaaagcg
     1141 atcttccgat cggcgagctc cgctgccgca aggtgctgat taactgttgc aacggcttcc
     1201 gccgagatgc caagccgtgt tcgcggccta tcactaaccg gcacgccggt ccagccgagc
     1261 agcgcgagtt gttgttcgac cgagtgagtt ttctgttgtc cccaaagtaa cagcggagac
     1321 ggcgccagtt gcgtgtgcaa cttcgcgtat tggtaggttt tgaaaccgac tctgtgtcgc
     1381 gcgccggtag cgcgggttaa gaaagtcgcc gttgtgccgc cgtgaagatt gtagaccacg
     1441 tcgtaacgcg ctgcgcgtag ctcgcgcgcg acacgcgtcc gtgcgactaa accgccgcgt
     1501 tcaagcacga caacgttgtc cacatgcgga tgatctgtga gcaatggtgc gacccactct
     1561 tcgacgagta tgtcgactcg cgcctgcggc aaaaaacgtt tcaaagcgaa gagtgaaggc
     1621 gttgccaaaa ccgtgtcgcc aattgaccgc aaccggacga tcagtacgcg ttggacggat
     1681 tcccagttcc accgaggcgg tgcgagtggt tgtggcgcgt cttcccgaaa cgccggttcc
     1741 gtcttcacag caccgaattg cggagaagac tcgactatgt cgcgcaggct gttcatcagt
     1801 tcattcttcg atcgtggctt cgtcgatgag catgaccgga atgtcgtcgc ggatcggata
     1861 gacgcgtttg cactcgacgc atttcaaagc gttgccgtcg ggcttgagtt ccacttttgc
     1921 tttgcaagcc ggacaggcga gtatttccaa gagttcctga ctaactgcca tgggcatgac
     1981 ctttctatgc ggcactgctg cggaaaagtg acaaaagctt aacataaagg cactggcttg
     2041 ctcgatgagc gaggctgtgt tagtgatttg agccacgggt tacacggatg tcacttgagt
     2101 tcgcattgca atacgcgtgt ccgttgcggg caagttattc tgaacgacac ttgcgcgagt
     2161 ggggccgctt gagcagcttg cacgcaaagt taaccactgg cgatgccgtc actccgtcca
     2221 tcgacaaact cgagtgggtg gaaaaggcca gtcccgagat cgagcgaatg cagcgggcga
     2281 cgacgatctg tgcgcattgt ccggcttgtc tgccgctcga tattgcggga gatggtgagt
     2341 cagtcgggtg tttgggtcgc attaactatc cgatcgaggc ggtgtttgaa aagtttctgg
     2401 ccgaccgcgt acagctttcg ctcgacacgc tcgacaaaga agaccagccg cggctgttgc
     2461 gcattctggt cgatccggag tcgccgtttg atggcgaggc gacgaaagag ttgcggcggg
     2521 tcgtgaccga gcagggttta cgtttctttg aactgcgcgt gccgatagtg ctcacgcgcg
     2581 aggcggcacg gctcagcact gataacgttt tcgacctgct ggcaggcttt cgttcggagg
     2641 atacccagca aactacctac acgcgcgagc ttcccttcga agcggccgcc gactactacg
     2701 actttctcga tttgatcctg cgt
//
LOCUS       DASTEY010000369         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_535496, whole genome shotgun sequence.
ACCESSION   DASTEY010000369 DASTEY010000000
VERSION     DASTEY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535496"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFO72_06440"
     CDS             <1..868
                     /locus_tag="VFO72_06440"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HET9478962.1"
                     /translation="PLHNPANIKGIVAARALFGEGLPQVAVFDTAFHQTLPDHAFLYA
                     LPYQLYRRYRVRRYGFHGTSHRYVAYRYRQLRGLQRDQTNIITLHLGNGCSAAAIRNG
                     NSIDTSMGLTPLEGLVMGTRCGDMDPAIIDFISTKEGLSSQEVETLLNKQSGLLGISG
                     LTSDMRELLAEAHEENDRRARLAIEIFCYRARKYIGAYLAAMNGADAIVFTGGIGENS
                     SEVRSRVCDGLQWLGLELDEDRNRAHVGGREGVISNDSRLAAYVIPTDEELLIARDTA
                     RCVLQPLKVKQK"
     gene            1077..1811
                     /locus_tag="VFO72_06445"
     CDS             1077..1811
                     /locus_tag="VFO72_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9478963.1"
                     /translation="MLSFRVTTYNIHKCRGMDRRIRPKRIAEVLREINADVVALQEVV
                     GMDEATRELNQVRSIAEELDLDFRIGENRRLRGAAYGNAILSRFPVFNDHNHDLSWRM
                     NEPRGCLEVGITLPGSDSQTVLQLFNVHLGTGFFERRYQAHRLLEVIAGDPSSPLPRI
                     ILGDFNEWTRGLTTRLLNLHFNSAEPEQRLGRARTYPGVFPLLHLDHVYYNSLLKLKS
                     ISVHRSRLALAASDHLPIVADFTLDE"
     gene            complement(1814..3298)
                     /locus_tag="VFO72_06450"
     CDS             complement(1814..3298)
                     /locus_tag="VFO72_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9478964.1"
                     /translation="MPRARRYQRERNAMLPVRDLANQAFSRAAGAPLIGGNSVRLLLD
                     AAENYPAWLDAIKAAERYVHFESYIIHEDREGWKFADALVAKAREGVRVRLVYDWMGG
                     LGKTSRSFWKYLRAGGVEVRCYNPPELDSPFGWISRDHRKTLTVDGLVGFVTGLCVGR
                     MWVGVPEKKIEPWRDTGIEVRGPAVADIEQAFANVWAVTGEDLTADEIAKREELEEAG
                     DVTLRVVATEPASAGMLRTDLLVATLARRRLWLTDAYYAGTNSYVQALRAAAKDGVDV
                     RLLVPSASDIPLVKPLSRAGYRPLLEAGVRVFEWNGTMLHAKTAVADCHWARVGSTNL
                     NIASWFGNLELDVVIEDKPFATEMEETYVQDLENATEVVLDARHKLRKPTKRESAQPA
                     ATSGGGSGSRAAAGAIRLGNAVGAAFTNRRVLEPVEARLMVTAGFFLFGVSLLTWFFP
                     RVLTYPLIVLMLWISLALFYRAYRLKQNEPVASANPETVETREL"
BASE COUNT          798 a         1014 c          966 g          793 t
ORIGIN      
        1 gccactgcac aacccggcga acattaaagg catcgttgcc gcacgcgcgc tgtttggcga
       61 agggttgcca caggtcgccg tgtttgacac tgcgtttcat cagacgttgc cggatcatgc
      121 ctttctgtat gctctgccct atcaacttta ccggcgctac cgagtgaggc ggtacggttt
      181 tcacggcacg tcgcatcgat atgtcgctta tcgttatcgt caactgcgcg gcctgcagcg
      241 agaccagacc aacatcatta cgcttcatct tggcaacggc tgttccgcgg ccgcaattcg
      301 taacggtaat tcgatcgaca cgtcgatggg cctgacgcca ctcgaaggtt tggtaatggg
      361 aacgcgttgc ggagacatgg atccggcgat catcgatttc atttcgacga aagaggggtt
      421 gtcgtcacaa gaagtcgaaa cactgctcaa taaacagtca ggattgctcg gaatctccgg
      481 gctgacgagc gacatgcgcg aactgctcgc tgaagcacat gaagaaaacg atcggcgcgc
      541 gcggctggcg attgagatct tctgttatcg cgctcgcaaa tacatcggag catacctggc
      601 agccatgaac ggcgcagatg cgatcgtgtt taccggtggg ataggagaga actcatcgga
      661 ggtacgctca cgcgtctgcg atggtcttca atggttggga ctcgagctcg acgaagatcg
      721 aaatcgcgcg cacgtcggcg gtcgcgaagg tgtgatcagt aacgattctc gtctggctgc
      781 ctatgtaatc ccgactgacg aagaactgtt aatcgcccgc gacacggcgc gctgtgtcct
      841 gcaaccgttg aaagtcaaac agaaataggg ggttgcctca ggagtcatct cattctcacc
      901 cggcttcagc cgggtgcctt ggcaaagctc ctgttcggaa accgttttaa cggtttccgc
      961 gtgtgaagcg atcagaaaac cgttgaaacg gttttcttcc ttccagcagg caaaacaccc
     1021 ggctgaagcc gggtgagaat gaaatcactt ttgaggtaaa gtccggaaat accaccgtgt
     1081 tatcgtttcg cgtcacaacc tacaacatac acaaatgtcg cggcatggac cgtcgtatac
     1141 gtcctaagcg aattgccgag gtgctgaggg aaatcaatgc ggacgtggtc gcgcttcagg
     1201 aagtggtcgg tatggacgaa gccacgcgcg aactcaacca ggtgcgctca attgctgaag
     1261 agctggactt ggatttccgc attggtgaaa accgcagact gcgcggtgcc gcatacggca
     1321 acgcgattct cagtcgattt cctgttttca acgatcataa tcacgatctt agctggcgaa
     1381 tgaacgaacc acgcggctgt ctggaagtcg gcattacgct tcctggtagt gactcgcaaa
     1441 cggtgctgca actcttcaac gtgcacttag gaacgggctt ttttgaacgg cgttaccagg
     1501 cccacagact gctcgaagta atcgccgggg acccgagttc ccctttgccc agaatcattc
     1561 ttggcgattt caatgaatgg acgcggggtc tgacaactcg tcttctgaac ctgcacttca
     1621 acagtgccga accggaacaa cgtctcggca gagcccgcac gtatcctggc gtgtttccac
     1681 tcttgcactt ggaccacgtc tattacaact cgctgctaaa actgaagagt atttcggtcc
     1741 atcgcagtcg tctcgcgctg gctgcttccg atcacctgcc gatagttgcc gacttcactc
     1801 ttgatgaatg aaatcataat tctcgtgtct cgactgtttc agggttagca ctggcgactg
     1861 gttcgttctg tttcagccgg tacgctctgt aaaagagtgc gagcgagatc cagagcatta
     1921 gaacaattaa gggataggtc agtacgcgag gaaagaacca cgtgagcaag gaaacaccga
     1981 agagaaaaaa tcctgcagtg accatgaggc gtgcctcaac cggttcgagc acgcgacgat
     2041 ttgtaaacgc tgcgcctaca gcgttgccaa gccgaatcgc gcccgccgcc gcacggctac
     2101 cgctgccgcc accgctcgtc gccgctggct gcgcagactc tcgtttggtc ggttttcgta
     2161 gtttgtggcg agcgtctaaa actacctcag tcgcattttc gagatcctga acgtacgtct
     2221 cctccatctc cgtcgcaaac ggcttgtctt caataacaac gtctagttcg aggttaccaa
     2281 accaacttgc aatgttgaga ttcgtcgaac caacgcgtgc ccagtgacaa tcggcaacgg
     2341 ccgtcttcgc atggagcatt gttccgttcc actcaaagac gcgcacacca gcttcgagca
     2401 aaggacgata acctgcgcgc gacaaaggtt tcaccagagg aatgtccgaa gcacttggta
     2461 cgaggagacg aacgtcaacg ccgtcttttg cggctgccct cagtgcctga acgtatgagt
     2521 tggtgccggc gtaataggca tcagttaacc acaatcgcct tcttgcaagt gtggcgacca
     2581 ggagatcagt gcgcagcatt ccggcgctgg cgggctccgt cgccacgacg cgaagcgtta
     2641 cgtcgccggc ttcctccaac tcttcgcgtt tcgcgatttc atcggcagta agatcctcgc
     2701 cggtaaccgc ccagacgtta gcgaatgcct gttcgatatc ggcgactgct ggtccacgta
     2761 cctctatgcc cgtatcgcgc cagggttcta ttttcttctc cggcacgcca acccacatgc
     2821 ggccgacgca caatccggtc acaaaaccga cgagtccatc tactgtcaaa gtcttccgat
     2881 ggtcgcggct gatccagccg aacggtgaat ccaattctgg tgggttgtaa caacgcactt
     2941 cgacgccacc cgcgcgcaga tatttccaga aagagcgaga agtcttgccc aagccgccca
     3001 tccagtcgta cacgagacgt acccgcacgc cttcgcgggc tttcgcgacg agcgcgtcgg
     3061 caaacttcca gccttcgcgg tcttcatgaa ttatgtagct ctcgaagtga acgtaacgct
     3121 cggcggcttt gatcgcgtcg agccatgcgg ggtaattctc cgcggcatct agtagtaaac
     3181 ggacggagtt gcctccgatg agcggtgcgc cggcagcgcg cgagaaagcc tggtttgcca
     3241 ggtcgcgcac tggcaacatc gcgtttcgct cgcgctggta tcgacgggca cggggcatct
     3301 ggcttattct cccccattcc gcgttaccag aacagtccgc tgcgaacaaa ccggtgcggg
     3361 cgttggtagt gtcctaatct gaacgccaga ggcgttcgct aattccagcc cagggttgct
     3421 tcaaccctgg gtcgaacgtt tgagtcccgt cctaacgctg aaaggcgttg gcaaattcgc
     3481 ggttagccaa cgctttcagc gtaaaaggta aaaacatcca atcagttccc agggttgtcg
     3541 caagctccaa cctctgggct ggaattcgat a
//
LOCUS       DASTEY010000370         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1376164, whole genome shotgun sequence.
ACCESSION   DASTEY010000370 DASTEY010000000
VERSION     DASTEY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376164"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFO72_06455"
     CDS             <1..1089
                     /locus_tag="VFO72_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HET9478965.1"
                     /translation="FLGGEPINVLRLREWAAHCQAEIVNTYGPTEATDTCSAFRLIKF
                     DHSTSAPPIGKPNDNVELLILDEHLNPVPQGVTGELCVGGRGVGRGYLNNPSATAERF
                     IPHAYSSVPGARLYRTGDLVRHLPDGNIEFLGRLDHQVKVAGYRIELGEVESMMVQHR
                     AVRECVVVVRPDAHGNARLVAYVVPEQDAQLPDLTALRRYLADSLPLHMIPSVFVSLE
                     KLPLTPGGKVDRRALPAPNTIGNGTGRDYVAPRTAVEEVLAEIWREVLAVERVGIHDN
                     FLELGGHSMLAMRCVSGIRQLFRIELPLRVLFETATLEELAQALKAYEDQPGALEKIA
                     RVFQKVKGVSREELHKQLLKRRASKAGK"
     gene            1200..>2971
                     /locus_tag="VFO72_06460"
     CDS             1200..>2971
                     /locus_tag="VFO72_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9478966.1"
                     /translation="MKMRELSNEIGVSAEELELFEYLLEEEGLAEVSPERVIPRRKQH
                     DDIPLSFAQERLWFLDQLEPGGAAYNMPGAVWLEGDLNLVALERSFNEIIRRHDVLRT
                     TFRAERGRPVQVIAPSMHLSLPLKDLSQFPDSESEVLRLEREESQRPFDLERGPLLRV
                     TLLRLAAEKHLLLVTLHHIIADGWSVMLLVQELGALYAAYSRGEQSPLAELEVSYADY
                     AVWQRGWLKGERLERELAYWREELRGELPVLKLPLAQLRAPEARHRGGLETVRVSREL
                     TQRLRELGRGAGATLFMTLLAAWKVLLSRLTGQRDVLVGTPVAGRDSRDLEGLIGFFV
                     NTLALRTDLDGAPSFTEVLRRVREVCLGAYVHQEVPFERVVEELAPQRELSHGPLFQV
                     LFALQNAPLPQIDLPGLRMRVEDATSATTKFDLALEITEDADELACVWQYDSDVFDAT
                     TIQRMAAHYQTLLSGIVADPACSIHELRLLSNDEERAILSEYNATHVTYPQQTLLHEL
                     FEEQAAITPDATAVVFEDVRLTYAELNRRANAVAHHLRSLKLLPEGRVGVLLERSADL
                     VVALLGVLKVGGVCVPLDPGYPSER"
BASE COUNT          605 a          764 c          959 g          643 t
ORIGIN      
        1 tttctcggtg gcgaaccgat caacgttttg cggttacgtg aatgggctgc gcactgccag
       61 gcggaaattg taaataccta tggaccaact gaagctacgg atacgtgttc cgcttttcga
      121 ttgatcaagt tcgaccactc gacaagcgcg cctccgatcg gcaaaccgaa cgataacgtc
      181 gagttgctga ttctggacga gcatttgaac cctgttccgc agggcgtcac cggagagttg
      241 tgcgtgggag gccgcggtgt tggcagaggt tatctgaaca atccgtcggc gactgcggaa
      301 cgtttcatcc ctcatgccta cagttccgtg cccggcgcgc ggctctatcg cacgggcgac
      361 ttagtccggc acttgcccga tggaaacatc gagtttctcg gtcgtctcga tcaccaggtt
      421 aaagtggctg gctaccgtat agaactgggc gaggtagagt cgatgatggt ccaacatcga
      481 gccgtgaggg agtgcgtggt cgtagtgcgg cctgacgcgc acggcaatgc gcgactcgtc
      541 gcttatgttg tgccggagca ggacgctcaa ctgccggatc tgacggcgct gcgtcgttat
      601 ctcgcagaca gtttgccgtt gcacatgatc ccgtcagtgt tcgtgtcact tgaaaaactg
      661 ccgctaacgc ctggcggaaa ggtggaccgc cgcgcactgc ccgctccgaa cacgataggc
      721 aatggaaccg gacgggatta tgtggcgcca cgcactgctg tagaggaggt tctcgcggaa
      781 atttggcgcg aggttctcgc agttgaacgc gttggaattc acgacaactt tctcgagctc
      841 ggcggccact cgatgctcgc aatgcgttgc gtctcaggga tacgtcaact gtttcgcatc
      901 gagcttccgc tacgagtcct gttcgagacc gcgactctcg aagagttggc ccaggcgctg
      961 aaagcgtacg aagaccagcc gggagctttg gagaagattg cgcgcgtgtt tcaaaaggtc
     1021 aagggagtct cgcgggaaga gttgcataaa cagttgctga aaaggcgagc aagcaaagcc
     1081 ggaaagtagt gcttagttcg gacttttctg tgcgctctgt gtctctgtgg ttatactttt
     1141 tgtacgacat aacaaccaca aagacacaga gcgcacagag aagatcgtaa gcagcgttaa
     1201 tgaagatgcg tgaattgtcg aatgagatcg gcgtttcagc cgaagagtta gaactgttcg
     1261 agtacctgct cgaagaggag ggtctggccg aagtctctcc cgagcgggtg attccgcgtc
     1321 gtaaacaaca cgacgacata ccactctcgt ttgcgcagga gcggctatgg ttcctggacc
     1381 aactggaacc cggcggtgcc gcttacaaca tgcctggcgc tgtgtggttg gaaggcgact
     1441 tgaacctggt cgcgctggaa cgaagtttta atgaaattat tcgccggcat gatgtcttgc
     1501 gcacgacgtt ccgcgctgaa cgcgggcggc cggtgcaggt gattgcgccg tcgatgcacc
     1561 tctctttgcc gctgaaagat ctgtcgcagt tccccgatag tgaaagtgaa gtgctgcggc
     1621 tggagcggga agaatcacaa aggccgtttg acctggaacg tggtccactg ttgcgggtga
     1681 cgctgctgcg cttggcagcg gagaagcacc tgttgctggt aacactgcac cacatcattg
     1741 ccgacggctg gtccgtgatg ctgttggtgc aggagttggg agcgctgtac gcggcctaca
     1801 gccgagggga gcagtcgccg ctggcggagt tggaagtgag ctacgccgac tacgcagtat
     1861 ggcagcgagg gtggctgaag ggtgagcgtc tggaaaggga gttagcttac tggcgcgagg
     1921 agttgcgagg agagttgccg gtgctgaagt taccgctggc gcagctgcga gcgccagagg
     1981 cgcggcatcg aggcggactg gagacggtgc gtgtcagccg cgagttgacg caacggttgc
     2041 gagagttggg gcgaggtgca ggagcaacgc tgtttatgac gctgctcgcg gcgtggaagg
     2101 ttttgctgtc tcgattgact ggccagcgag acgtgttggt gggaactccg gtggctgggc
     2161 gcgacagccg cgatttggaa gggctgatcg gatttttcgt gaacacgctg gcactgcgca
     2221 cggacttgga cggcgcgccg agtttcacgg aggtgttgcg gcgcgtgcgg gaagtatgtc
     2281 tcggagccta cgtgcatcag gaggtgccgt tcgagcgtgt agtcgaggag ctggcgccgc
     2341 agcgagagtt gagtcatggt ccattgtttc aagtgctctt cgctttgcag aacgcgccgt
     2401 taccgcagat cgatttgcct ggactccgta tgcgcgtcga agacgccaca agcgcgacga
     2461 ccaaattcga tctggcgctg gagataaccg aagatgcaga cgaactggca tgcgtctggc
     2521 aatacgattc cgatgtcttc gatgccacaa cgatccagcg tatggccgcg cattaccaga
     2581 ctttgctttc gggcatcgtt gccgatcctg catgcagcat tcacgagctg aggttgctga
     2641 gcaacgatga ggagcgtgcg atactcagtg aatacaatgc cacccatgtt acctacccac
     2701 agcagaccct tcttcatgaa ctgtttgagg agcaagctgc aatcacacca gacgcgactg
     2761 ccgtcgtctt cgaagacgtt cgcctgactt atgcggagct taaccggcgc gcaaacgcgg
     2821 ttgcgcatca cctgcgaagc ctcaagctac ttccggaggg acgcgtcggt gtactgctgg
     2881 aacgttccgc ggatctcgtc gtggctttgc tgggggtgct gaaagtgggt ggcgtctgcg
     2941 tgccgctcga tcctggttat ccatccgagc g
//
LOCUS       DASTEY010000371         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1359817, whole genome shotgun sequence.
ACCESSION   DASTEY010000371 DASTEY010000000
VERSION     DASTEY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359817"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFO72_06465"
     CDS             <1..852
                     /locus_tag="VFO72_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9478967.1"
                     /translation="GDAHLATGALAEAEEQYLFALEGRLENAAALLGLAQISRLKGEI
                     KEATLYLTRVGTLTADSKSPEFLYKFAAVAMQVGMSDAAKSALENALKLRPNEPAYLL
                     AYGIAWLRKGDLFEAEKVFRRLVQLQPNSDQAQIHLGYVLLNQKKYDEARLWLEKSAR
                     STAPMPEVFYYLGLVAQEQNDDAKAIALFEKAVQRLPNYAHARIALGSSYIKLRNYPR
                     AKEELETAVKLDPEEPTAHYNLALLYARLKDPARAQEEMRIVEKLKAKGAGSDGVVVL
                     PPNPNPR"
     gene            856..2478
                     /locus_tag="VFO72_06470"
     CDS             856..2478
                     /locus_tag="VFO72_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HET9478968.1"
                     /translation="MKSVFLALTLLLSVTFEEVPPQTSKITWIHNNAQSADRQLPETV
                     GAGCAFLDYDNDGWMDIYFVNSGPSDFFTPTTPLKNALYRNNRDGTFTDVTDKAGVAG
                     GKFGMGVAAADFDADGFVDLYVTNYGPNILYRNNGNGTFTDVTDKAGVASPGWSTCAT
                     WFDFDNDSKLDLFVSSFVFYDKSQNPLCTDPTLQRRYYCVPRLFKPTPSRLFRNNGNG
                     TFQDVSRESGIADHPGKSFGAVATDVNNDGLMDLFVANDTMPNFLFVNKGSGKFEEVG
                     LAAGVAYGEAGRPRSGMGVDAADYDGDGWQDLFVANIDHEFFSLYHNDKELIFTDQPG
                     EIAPATQLLSGWGLKFFDYDNDGDPDLFLVNGHPDDMIESRIPRVKHREPLLLFENTG
                     RGFKDVSATSGSVFGKVFSGRGMAVGDFDNDGDSDVLASNNGEPPLLLRNHGGNKNNW
                     VGLELVAKKSNSAAVGAVITWQAGTMRRTRLRTGGGSYLSSHDPREILGVGTATKIDS
                     VQIRWPSGTTDRLTNVPLNKYIKVVEGVGQAR"
     gene            2498..>2752
                     /locus_tag="VFO72_06475"
     CDS             2498..>2752
                     /locus_tag="VFO72_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478969.1"
                     /translation="MIVVLGQTQQDTFFSEWRPNTNTGGIRYVGNNACVDCHKEQAGQ
                     LHTPMGQALEVAPDCRIVTAKGKLTFRNGPYTYNLAREGSQ"
BASE COUNT          628 a          792 c          774 g          558 t
ORIGIN      
        1 ggcgatgcgc acctcgctac cggcgcgctc gccgaagctg aagaacagta tctattcgcg
       61 cttgaaggcc gtctcgagaa cgccgccgcg ttgctcggtc tcgctcagat ctcacgactc
      121 aaaggcgaga tcaaagaagc gacactttac ctgacgcgtg tcggcacgct tacggccgat
      181 tcgaagtcgc ccgagtttct ttacaaattt gccgccgtcg caatgcaagt gggaatgagt
      241 gatgccgcaa aatccgcact cgaaaatgcc ctgaaactgc ggcccaacga accggcttac
      301 ttactcgctt acggaatcgc gtggctgcga aaaggcgatc tgtttgaagc ggagaaagtg
      361 tttcgccgcc tggtccaact gcaaccgaac agcgatcagg cgcagatcca tttgggctat
      421 gtgctgctga atcagaagaa atacgacgaa gcacgactct ggctcgagaa gagtgccaga
      481 tcgactgcgc cgatgccgga ggtgttctac tatctcggcc tcgtcgcgca ggagcagaac
      541 gatgacgcga aagccatcgc attgtttgaa aaggccgtgc agagacttcc gaactacgct
      601 cacgcgcgca tcgcgctcgg ttcgagttac ataaaactgc gcaactaccc acgcgcaaaa
      661 gaggagttgg aaacggcagt caaactcgac ccggaagaac ccacagcaca ctacaatctc
      721 gcactgctgt acgcccgctt gaaagatccg gcacgcgctc aggaagagat gcgcatcgtg
      781 gaaaagctga aagcgaaagg cgccggaagt gatggcgtgg tggtgctgcc gcctaacccc
      841 aatccgcgtt gatctatgaa gtccgtgttc ctggcactta cacttcttct ttcggtgacc
      901 ttcgaagagg tcccgccgca aacaagcaag atcacctgga ttcacaacaa cgcccagtca
      961 gcagatcgcc agttgcccga aactgtcggt gcgggttgcg ctttcctcga ctatgacaac
     1021 gacggctgga tggacatcta tttcgttaac agcggtcctt ccgatttctt cacgcccacg
     1081 acgccattaa agaacgcact ctaccgcaac aatcgtgacg gaactttcac cgacgtcaca
     1141 gacaaagcgg gcgttgccgg tggaaagttt ggcatgggcg tagccgcagc ggatttcgac
     1201 gcggatgggt ttgtcgatct ttacgttacg aactacggac caaacattct ctatcgcaac
     1261 aacggcaacg gcacattcac cgacgtcacc gacaaagcag gcgtcgcctc gcccggttgg
     1321 tccacctgcg cgacctggtt tgacttcgac aacgacagca aactcgatct gtttgtctcg
     1381 agcttcgtct tttacgacaa gtcgcagaac ccgctttgca ccgacccgac gctgcaacgc
     1441 cggtactact gcgtgcctcg tcttttcaaa ccgacgccat cgcgtctgtt ccgcaacaac
     1501 ggcaacggta cgtttcaaga cgtgagccgc gaatccggca tcgctgatca tcccggcaaa
     1561 tcatttggcg ctgtcgccac tgacgtgaac aacgacggct taatggatct tttcgtcgcc
     1621 aacgacacga tgcccaactt cctcttcgta aacaaaggca gcggcaagtt tgaggaggtc
     1681 ggtttggccg cgggtgtcgc ctacggtgaa gccggccgcc cgcgttcggg aatgggtgtt
     1741 gacgccgccg attacgacgg cgacggctgg caggatcttt ttgtcgccaa catcgatcac
     1801 gaattcttca gtctctatca caatgacaag gagttgatct ttaccgatca gcccggcgag
     1861 atcgcgccgg caacgcaact gttgagcggt tggggattga agtttttcga ctacgacaac
     1921 gacggcgatc cggatctgtt tctcgtcaac ggccatcccg acgacatgat tgagtcgcgc
     1981 attccgcgcg tgaagcacag agagccgctg ttgcttttcg aaaacaccgg ccgcggtttc
     2041 aaagacgtga gcgcgacttc cggcagcgtg tttggcaaag tgttttccgg gcgcggcatg
     2101 gcggtcggtg atttcgataa cgacggcgat tccgacgtgc tggcatcgaa taacggtgag
     2161 ccgccgctgt tgctgcgcaa tcatggtggc aacaaaaata attgggtggg cctggagctc
     2221 gtggcgaaga agagtaattc cgcggcggtc ggcgcggtga tcacgtggca ggcgggaacg
     2281 atgcgacgca cgcgtttgcg caccggcggc ggcagctatc tttcgtcgca cgacccgcgc
     2341 gagattctcg gcgttggaac tgcgactaag atcgattcag ttcagatccg ttggcccagt
     2401 ggcacgacgg atagactcac aaacgtaccg ctcaacaaat acattaaggt ggtggaaggt
     2461 gtggggcagg ctcggtaaaa tctgtgtatt ctgtggattg atcgtggttc tcggtcagac
     2521 ccaacaggac accttcttct ccgagtggcg gccgaatacg aacacgggcg gtattcgtta
     2581 cgtcggcaac aacgcctgcg tcgattgtca caaggaacag gccggacagt tgcacacgcc
     2641 gatgggccag gcgctcgaag tcgcgcctga ttgccgcatc gtgaccgcca aaggcaaact
     2701 cacgtttcgc aacggccctt acacttacaa cctcgcgcgc gaaggctcgc ag
//
LOCUS       DASTEY010000372         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1639899, whole genome shotgun sequence.
ACCESSION   DASTEY010000372 DASTEY010000000
VERSION     DASTEY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639899"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFO72_06480"
     CDS             <1..1080
                     /locus_tag="VFO72_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9478970.1"
                     /translation="TNVQRAYWDLVFALRSLQVQRDAVSVAQTQLEHNKRLVQEGQLA
                     PIDIVAAEAQIFTYEQGVFGALEEVSRAENNLKNLIAENKQSSIWTEAVVPIDPVELT
                     VPEVTLPDALKMAMENRPELQQSNVVKEINEIDQKYFKNQTKPAVDLVGQYGINGLAG
                     SISAAGVNPFTASSLVVRQRVDELSALAGLDPLPVVPPQTLSPELLGGFGQSVQNLLS
                     NRFNNFRVGVQISLPLRNRTAEAQLGRSLVEGERIATQREQLEQSIEVEVRNSLQAVR
                     SAESRLRAAIATREANEQQFASEQRKLDAGQSTTFLVLERQTNLTTARGLELKAQTDL
                     NKAIADLQRATGNALRVNSVVVRVR"
     gene            1096..1575
                     /locus_tag="VFO72_06485"
     CDS             1096..1575
                     /locus_tag="VFO72_06485"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9478971.1"
                     /translation="MAKNMSINQLVETWQIHDRINLYLLDAIAPESLNSHSASKGRSV
                     GEQFAHLHNVRLMWLKSAAPELLHGLNKIENEQASDKKLLAKSLADSGKAIGALLAQS
                     LEAGGKVKGFKPHAAAFLGYLISHESHHRGQIALTMKQAGQPLDRKIGYGIWEWGVR"
     gene            1632..1892
                     /locus_tag="VFO72_06490"
     CDS             1632..1892
                     /locus_tag="VFO72_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478972.1"
                     /translation="MRQRRTRRAAGFGKFGNAFVSNSRAVHSYGKSFVYLVTMQFETT
                     NRLPGTAGVSPASDGSVRLVTVQARRLRSQQEGASVFKRVYL"
     gene            complement(1926..>2748)
                     /locus_tag="VFO72_06495"
     CDS             complement(1926..>2748)
                     /locus_tag="VFO72_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9478973.1"
                     /translation="ANDPVPDSLLLESGKAAILRTHTSDIREVKIVSYNIRWRGGDEL
                     KQLAKLLHEDPEIGGAAILGLQEVDRRKKRTGHRNTVKALADELGLHYAWAAPPAAKP
                     QDEEETGVAIMSVYPLSDVRRIVLPHAGPNRRRRVALGATVEIDGRQWRVYSAHAETR
                     LSLKKKMEQYNAILEDLARYPADTPAVVMGDLNTWEPSADDKTIRLFSKAGMTTPFGT
                     QSTFRRRIVFVPIELRLDWVWLRGLEATSHGIDRQINHSDHWPLWTNVRMVATKR"
BASE COUNT          629 a          743 c          793 g          583 t
ORIGIN      
        1 accaacgtgc agcgcgcgta ttgggatctc gtgttcgcgc tgcgcagcct ccaagtgcaa
       61 cgcgatgctg tgtccgttgc ccaaacgcag ctcgaacaca acaagcgtct ggtccaggag
      121 ggccaactcg cgccgatcga catcgtcgcg gcggaagcgc agatctttac ctacgagcaa
      181 ggcgtcttcg gtgccctcga agaagtatcg cgcgccgaga acaacttgaa aaacctgatc
      241 gccgagaaca agcagtcgtc catttggacc gaagcagtcg tgccgatcga tccggttgaa
      301 ctgactgttc cggaggtaac ccttccggat gcactgaaga tggcaatgga gaacaggccg
      361 gagttgcaac agtcgaatgt ggtgaaagag attaacgaga tcgaccagaa gtacttcaag
      421 aaccaaacga agccggctgt cgacctcgtc ggtcagtatg gtatcaatgg tcttgccggc
      481 tcaatcagcg cggcgggtgt gaatccgttc acggcgtcga gcctggtagt gcggcaaaga
      541 gtagacgaac tttcagcgct tgccggactc gaccccttac cggtggtgcc gccgcaaact
      601 ttgtctccgg agcttttggg cggtttcggg cagtcagtac aaaatctgct ctcgaaccgc
      661 ttcaacaact tcagagtcgg cgtgcagatc agtttgccgt tgcgcaatcg cactgctgaa
      721 gcgcaactgg gccgatcgct ggtggaaggc gaacgcattg cgacgcaacg cgaacaactc
      781 gaacagtcga ttgaagtcga agtgcgaaat tcgttgcagg cagtgcgcag tgctgagtcg
      841 cgtttgcgtg ccgcgatagc gacgcgcgag gcgaacgaac agcagtttgc cagcgagcaa
      901 agaaagttgg atgcgggcca gtccacgacg tttctcgtgc tcgaacggca gacgaacttg
      961 acaacggcac gcggcctgga gttaaaagcg cagaccgatt tgaataaggc aattgccgat
     1021 ttacagcgag cgacgggaaa tgctttgcga gtaaacagcg tcgtagtaag ggtccgttaa
     1081 agtctgtgtt tatctgtggc taagaacatg tcaattaacc aacttgtcga gacctggcag
     1141 atccacgatc gcatcaacct gtatctgctc gacgcgattg ctccggaatc gctgaacagt
     1201 cattcggcgt ccaaaggcag aagcgtcggc gaacagtttg ctcacctgca caacgtgcgg
     1261 ctgatgtggt tgaagtcagc tgcgccggaa ctgctccacg gtctgaacaa gatcgagaac
     1321 gagcaggcga gcgacaagaa actgctggca aagtcgttgg ccgattccgg caaggcgatt
     1381 ggagcgctgc tcgcacagag tttggaagca ggcggaaaag taaaaggatt caagccgcac
     1441 gcggcagcgt ttctcggtta cctgatttca catgagtcac atcatcgggg gcagatcgcg
     1501 ctgacgatga aacaggcagg acagccactc gatagaaaga tcggttacgg aatatgggag
     1561 tggggcgtca ggtgactaaa agaggttctc gataagcatg aaggcgccat agattccaag
     1621 acagataaga attgcggcaa cgacgtacca ggcgggcagc cggctttggc aagttcggca
     1681 acgcgttcgt atccaactcg cgcgccgtcc acagttatgg caagtcattc gtctatctag
     1741 taacgatgca gtttgaaact acgaaccgtt tacctgggac cgcaggcgtc tcgcctgcat
     1801 ccgacggcag cgtccgactc gtcaccgtgc aggcgagacg cctgcgctcc cagcaggaag
     1861 gcgcctcagt tttcaaacgc gtctatcttt gacaggattg cgtcgaaaca tcaagtcggg
     1921 gcaggttacc ttttagttgc gaccattcgc acgttggtcc acaacggcca atgatcggaa
     1981 tggttgatct ggcggtcgat tccgtggctg gttgcttcca gacctcgcaa ccatacccag
     2041 tcgagtctta attcaatcgg cacaaacaca atgcggcgtc taaatgttga ctgcgtgccg
     2101 aacggcgttg tcatgccggc tttggaaaac aatctaatcg tcttgtcgtc cgcgcttggt
     2161 tcccacgtat taaggtcgcc cattacgact gccggtgtgt cggcgggata acgcgcgagg
     2221 tcttccagaa tcgcgttgta ctgctccatc tttttcttca acgagagcct cgtctccgcg
     2281 tgggcagaat agacgcgcca ttgccggccg tcgatctcga ctgttgcacc caacgcgacg
     2341 cgacgcctgc gattcggacc agcgtgaggt aagactattc gtcgtacgtc tgacaacgga
     2401 tacacactca ttatcgcgac gcccgtttct tcttcatctt gaggtttagc cgccggcggc
     2461 gccgcccagg cgtagtgcaa gcccagttca tcagcgaggg ctttcacagt gttgcgatgg
     2521 cccgttcgct tcttgcgccg gtccacttcc tgcaacccga gaatagccgc gccgccaatt
     2581 tccggatctt cgtgtaggag ttttgccaac tgtttaagtt cgtcgcctcc gcgccagcga
     2641 atgttgtacg agacgatctt gacttcgcga atgtccgaag tgtgtgtgcg tagtatcgcg
     2701 gctttgcctg attccagcag gagcgaatca ggaacaggat cgtttgcc
//
LOCUS       DASTEY010000373         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1260793, whole genome shotgun sequence.
ACCESSION   DASTEY010000373 DASTEY010000000
VERSION     DASTEY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260793"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VFO72_06500"
     CDS             complement(<1..1555)
                     /locus_tag="VFO72_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478974.1"
                     /translation="MNKQAFTTLEYQHLLELIKRNAQTETGQRRVDALTPIDDVDALR
                     RELSALSECVTLRRRGVKWWFNGLSDPTEAIARLRVEGASLDPLAILQTARLCEQAMS
                     ARAAILAERENAPVLWQLVENLPRDLNSLVARVTNKILPSGELDDRASPELANIRHEI
                     NVLRSRITRSLERLMHRSAEAIQDELVTIRNDRFVIPVKADHRGRVQGVAHGYSSSGA
                     TAFVEPLETIDANNELQSLHESEAREIARILSGLSAELRAQLPAIEMAAEAVAELDFI
                     NARAVFHQTFNCVVPEILTRRDAYTSGHLELVEARHPLLEENLRATGGAVVPVSFALD
                     DEKNAMVISGANAGGKTVVLKTTGLLALMALSGISVPARSARFPFYASVLADIGDHQS
                     LAANLSTFTSHVANISRMIEVCEAPALVLLDEVGTGTDPEEGSALGVAVVDHFRRVCG
                     AHVIATTHYSGLKMYAGNEEGVINASVEFDEKTLRPTYRLVVGVAGASSGLEIARRFG
                     VPMGIVDSAM"
     gene            1646..1987
                     /locus_tag="VFO72_06505"
     CDS             1646..1987
                     /locus_tag="VFO72_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9478975.1"
                     /translation="MDEFDRYRAAMNEKLLGSNHLGIKRFFALDNQAYEDGALDKRTK
                     ELMGLVASAVLRCDDCITYHIKQCAAIGINREEFLDAFNVALVVGGSITIPHLRRAIE
                     RVDQLLENQPV"
BASE COUNT          481 a          651 c          597 g          463 t
ORIGIN      
        1 tcatcgcgct gtcgacgatc cccatcggca cgccaaacct gcgcgcgatc tccaatcccg
       61 acgaagcgcc agccacgccg acgaccaacc ggtaggtcgg tctcaaagtc ttttcgtcaa
      121 actcaacgct cgcattaatc acgccctctt cgttgcctgc gtacatcttg agtccgctgt
      181 aatgcgtagt ggcgatgacg tgcgcgccgc aaaccctgcg gaaatgatcg acaacagcca
      241 ccccgagcgc cgatccttcc tcaggatcag ttccggttcc gacttcatcc agcaggacca
      301 acgcgggcgc ctcgcacacc tcgatcattc gcgagatgtt tgcgacgtgt gacgtaaacg
      361 tcgagagatt tgcggccagc gattgatgat cgccgatatc cgccagcaca gacgcataaa
      421 acggaaaccg cgctgaccgc gccggcacgg aaattcctga caacgccatc aacgcgagca
      481 gacccgtcgt tttcaaaacg acagtcttgc caccggcatt cgcgccggag atcaccatcg
      541 cgttcttttc atcgtcgagt gcaaacgaga ccgggaccac cgcgccgcct gttgcgcgca
      601 ggttttcctc gagcaacggg tgccgcgctt cgactaattc caaatgcccg cttgtatatg
      661 catctctccg cgttagtatt tcaggaacga cgcagttgaa cgtctggtga aaaacggcgc
      721 gagcgttgat gaagtcgagc tcggcgaccg cttccgcagc catctcgatg gccggcagtt
      781 gcgcgcgcaa ctctgctgac aacccggaga ggatccgggc aatctcgcgc gcctcgctct
      841 cgtgcaggct ctgcaactcg ttattggcgt caatcgtttc gagcggctcg acgaaagcag
      901 tcgcgccgga agacgaatag ccatgggcca caccctggac ccgcccgcga tgatcggctt
      961 taacgggaat cacaaaacga tcgttacgga tcgtgacgag ctcgtcctgg atcgcttcag
     1021 cggacctgtg catcagtctt tcgagcgagc gcgtgatgcg cgagcgcagt acgttgatct
     1081 cgtgacgaat attcgccagc tcaggactgg cacgatcgtc gagctctcca ctcggcagga
     1141 tcttgttcgt tacgcgtgcg acgagcgagt tgagatcgcg cggtagattt tcaaccagtt
     1201 gccacaacac cggcgcattc tcgcgctcag cgagaatggc agcgcgtgca gacatcgcct
     1261 gttcgcaaag acgcgctgtt tgcaaaatag cgagcggatc caggctcgcc ccttcgactc
     1321 gcagacgcgc aatcgcttcg gttggatcac tcagtccgtt gaaccaccac ttgactccgc
     1381 gacgtctcaa cgtgacgcac tctgacaacg cggaaagctc gcgtcgcaaa gcatcaacgt
     1441 cgtcgatagg cgtgagtgcg tcgacgcgcc gctgtcctgt ttcggtctgc gcgtttcgtt
     1501 ttatcagctc cagcaggtgc tgatattcga gagttgtgaa tgcctgtttg ttcaagagct
     1561 aaaggttaaa gtattagcca cggatttgca cggatgcaca tggatcgtac aatgagggtc
     1621 caatttacct gaaagggcgc acgcaatgga tgagtttgac cgctatcgcg cggcgatgaa
     1681 tgagaaactg ctcggcagca atcacctggg gatcaaacgt ttctttgcgc tcgataacca
     1741 ggcttacgaa gacggcgcgc tcgacaaacg gaccaaagaa ttgatgggcc tggtggcgtc
     1801 agccgtgctt cgttgcgacg actgcatcac ctatcacatc aagcaatgcg ctgcgattgg
     1861 aataaaccgt gaagagtttc ttgacgcttt taacgtggcg ctcgtagtcg gcggatcgat
     1921 cacgattccg catttgagac gcgcgattga gcgcgtcgat caactgctcg aaaaccaacc
     1981 cgtgtaaacc gtgaaaactg cgttcttact actagtactt attttgatgg ctcagggtgt
     2041 ctccatgtct gcacaagaaa gaccacgtgt tcgcgcgccg gaaatcaccg gtggccgcgg
     2101 ttggctgaat actgacaagc cgctctcgct cgccgggttg aaaggcaaag tcgtcttact
     2161 cgacttctgg acctacggct gcatcaactg ca
//
LOCUS       DASTEY010000374         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_766574, whole genome shotgun sequence.
ACCESSION   DASTEY010000374 DASTEY010000000
VERSION     DASTEY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766574"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFO72_06510"
     CDS             <1..879
                     /locus_tag="VFO72_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9478976.1"
                     /translation="MQGLGTFAIARAAPDHVRDFWLSRAASGKAIAAFALTEPNAGSD
                     VAAIQTTARRDGESYVIDGRKRFISNAGIADFYTVFAKTGTREDGRPALSAFVVGSKM
                     QGFRVVERTPLIAPHPIGEIAFDQCRVPAEDMVGNEGDGFKLAMETLDTFRASVGAAA
                     CGMARRALEASLRYSSNRKQFGCLLSEHQLVQAKLADMITELDAARLLVYRAAYLRDT
                     ESGRFTREASEAKLFATEAAGRIIDSAVQIHGGAGLVSGTVVERLYRDVRALRIYEGT
                     SEIQKLVIAGQLLKES"
     gene            944..2077
                     /locus_tag="VFO72_06515"
     CDS             944..2077
                     /locus_tag="VFO72_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9478977.1"
                     /translation="MNINIIGGGPAGLYFAILMKKADASHRIKIYERNGPNDTFGWGV
                     VFSGKTLANLRAADETSHAEITKEFAAWDNVDVVHRSNKVSIHGNSFSGIARLHLLKI
                     LQRRADELGVEIEFHKESHNIEELRNDCDLLLAADGVNSTVRLNYVEQLQPELDLRTN
                     RYIWYGTNQLFHGLTLTFRENSAGVFAAHSYKFNPTTSTFIVECDPQTWDKAGFERMS
                     DADTRAYLGEVFAEDLQGHKLLSNNSKWINFLLVKNKNWFFENMVLIGDALHTAHFSI
                     GSGTKLAMEDAIALAESFQQKTNVSDALTHFTETRRPVILDYQAAAFESMLWFENAAQ
                     YMHLSPLELAFSLMTRSGRVSYEDLKRRDPDFIRAYESASIRG"
     gene            complement(2063..>2974)
                     /locus_tag="VFO72_06520"
     CDS             complement(2063..>2974)
                     /locus_tag="VFO72_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478978.1"
                     /translation="RPRELEALRFGYRERVTDTHLQHIRELPQELPGIFASAAERARV
                     AGFDGVELHYAHAYTMAGFLSAQNDREDGYGGSSENRVRLPLEVYREVRNRVGKDYVV
                     GVRFLADEVIAGGSRVEDAIYYAKQFAAAGFDFLSLSKGGKFEDAQQPKVGQAVYPYT
                     GQSGYECMPTVLSDAVGPFGRSVPLVAAIKEAVNEAGFKTPVVATGGISTFAEAEEIL
                     RKGQADIVGMARQALADPDWFIKVKLGRGDEVRRCTYTNYCEALDQAHRPVTCKLWDR
                     VDLDEPGIATVDEGRRRLEPPRWLATD"
BASE COUNT          702 a          866 c          778 g          628 t
ORIGIN      
        1 atgcagggcc tcggaacttt cgccatcgct cgtgcagcgc ccgatcacgt gcgggatttc
       61 tggctctcgc gcgccgccag cggcaaggcg atcgccgcgt tcgcgttaac agagccaaac
      121 gccggctcag atgtggcagc aatccagacg acggcgcgtc gcgacggcga gtcatacgtc
      181 atcgatggcc gcaagcgatt catttccaac gccggcatcg ccgacttcta caccgtcttc
      241 gccaaaaccg gaacgcgcga agacggtcgt cccgccctct cagccttcgt ggttggctca
      301 aagatgcagg gttttcgcgt cgtcgaacgg actccgttga tcgcaccaca tccaattggc
      361 gagatcgcgt tcgaccagtg tcgcgtgcct gcggaagaca tggtgggaaa cgaaggtgat
      421 ggtttcaaac tggcgatgga aacgctggac acgtttcgcg ccagcgtagg agctgcggct
      481 tgcggcatgg cccggcgggc acttgaagca tcgcttcgct attcatccaa ccgtaaacag
      541 ttcggatgtt tgttatctga gcatcaactg gtccaggcaa aactggctga catgatcacc
      601 gagctggacg ctgctcgact gctcgtctat cgcgccgcat acctccgaga cacagagagc
      661 ggtagattca ctcgcgaagc aagtgaagcc aagttgttcg ccacggaagc tgctggcaga
      721 atcatcgaca gcgcagttca aattcacggc ggcgccggcc tggtgagtgg aaccgttgtc
      781 gaacgtctct atcgcgacgt ccgcgcctta cgcatctacg agggcacatc agaaatccag
      841 aagctcgtca tcgccggtca acttttgaag gagagttagc cacaaaaatg cacaaaagac
      901 agaaatatga atttgtgtct ttggtgcctt tttgcggctt aatatgaaca tcaacatcat
      961 tggtgggggg ccggcggggc tttattttgc gattctgatg aagaaggctg acgcctcgca
     1021 tcggatcaag atatacgagc ggaatgggcc aaatgatact ttcggttggg gagttgtgtt
     1081 ctcggggaag acgctcgcta acctgcgtgc tgctgatgag acctcgcatg ccgagataac
     1141 caaagagttt gcggcgtggg acaacgtcga tgtcgttcat cgaagcaaca aagtttcgat
     1201 tcatggcaac agcttttccg gaatcgcaag actccatttg ctcaagattc tgcaacggcg
     1261 ggccgatgaa ttgggcgtcg agatcgaatt tcacaaagag agtcacaaca tcgaggagtt
     1321 gcgaaatgac tgcgatttgc tgctggccgc tgacggtgtg aacagcacgg tgcggctaaa
     1381 ctacgtcgag caattacagc ctgagctcga tctgcgcaca aatcgataca tctggtacgg
     1441 aacaaatcaa ctcttccacg ggctcacgct cacctttcgt gaaaacagcg ccggagtgtt
     1501 cgcagctcac tcgtacaagt tcaatccgac tacgagcact ttcattgtcg aatgcgatcc
     1561 gcagacgtgg gacaaagccg gattcgagcg catgagtgac gcagacacac gcgcgtatct
     1621 gggcgaggtc ttcgcggaag atcttcaagg acataagctg ctttcaaaca attcgaagtg
     1681 gatcaacttc ctgctcgtaa agaacaagaa ctggttcttc gaaaacatgg tgctgattgg
     1741 cgacgctttg cacaccgcgc acttctcaat cggctcaggc acaaagctgg ctatggaaga
     1801 cgcgatcgcg ctcgctgaat cgtttcagca aaagacgaac gtctcggacg cgctaacgca
     1861 ttttactgag acacgacggc ccgtgattct tgattaccag gccgcggctt tcgagagcat
     1921 gctgtggttt gagaatgctg cgcagtacat gcacctgagc ccgctggagc ttgcgttctc
     1981 attaatgacg agaagcggcc gcgtcagtta cgaagatttg aaacgtcgcg atccggattt
     2041 catccgtgca tatgaatccg cgtcaatccg tggctaacca acgcggcggt tccaggcgcc
     2101 ggcgaccctc atcgacagtc gcaatccccg gttcatcgag gtcaacacga tcccataact
     2161 tgcacgtcac tggtcgatgc gcctggtcga gtgcttcaca atagtttgta tacgtgcaac
     2221 gccgcacttc atcaccacgc ccgagtttca cttttataaa ccaatccgga tccgcgagcg
     2281 cctggcgcgc cataccgacg atatcagcct ggcctttgcg cagtatctct tcagcctctg
     2341 caaaggtcga tatcccacca gtcgcgacta ccggcgtctt aaaccctgct tcgttgactg
     2401 cctctttaat tgctgctaca agcggcacac tccgtccaaa tgggccaacg gcgtcggaaa
     2461 gcacggtcgg catgcactcg taaccgcttt gtccagtgta cggataaacg gcctggccca
     2521 ctttcggctg ttgcgcatcc tcaaacttgc cacccttaga aagcgacaag aagtcgaaac
     2581 cagccgccgc aaactgtttc gcgtagtaga tcgcatcttc aacccgactg ccacccgcaa
     2641 taacttcatc cgccaaaaac cgcacgccaa caacgtaatc tttcccgaca cgattgcgca
     2701 cctcgcgata aacctctaac ggcaaccgaa cgcgattctc actcgatcct ccataaccat
     2761 cttcacgatc gttttgcgca ctcaaaaacc ccgccatcgt gtaagcgtgc gcatagtgaa
     2821 gctcaacgcc atcaaacccc gccacacgcg cacgctccgc cgccgacgca aaaataccgg
     2881 gcaactcttg aggcagctcg cgaatgtgct gcagatgcgt gtcggtgacg cgctctcgat
     2941 aaccaaaccg cagtgcttca agctcacgcg gtcg
//
LOCUS       DASTEY010000375         6890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_140115, whole genome shotgun sequence.
ACCESSION   DASTEY010000375 DASTEY010000000
VERSION     DASTEY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140115"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFO72_06525"
     CDS             complement(<1..272)
                     /locus_tag="VFO72_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478979.1"
                     /translation="MKLTELDFSDFPPGTVTEYTTLVCLACIFDIFTKQIGLAPRFAY
                     SEIKRYSPTIEELTSPKAVRQFFDSEEKHPRCPYCNAAKRWHARLDT"
     gene            complement(275..1156)
                     /gene="ttcA"
                     /locus_tag="VFO72_06530"
     CDS             complement(275..1156)
                     /gene="ttcA"
                     /locus_tag="VFO72_06530"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET9478980.1"
                     /translation="MAFWRGSAMRCDDKTKTRLLRRVGEAISDFAMIEDGDRVMVCLS
                     GGKDSHTMLDLLLDIRRRAPVKFDLLAVNLDQKQPGFPAEVMPEYLSKLDVPFRIVER
                     DTYSIVKRLVPEGKTYCSVCSRLRRGVLYNIAVEEGCNKIALGHHADDIITTLLLNLF
                     YVGSLKAMPPKLRSNDGRNTVIRPLAYCSERDIADYASRCDFPIIPCDLCGSQSNLKR
                     VHMKKLVSHLEEEIPHVRASMLNALSNVVPTHLLDHRLFDFQTLASATGNVEAELDLA
                     VGHTDEDLRPALVTVSS"
     gene            1414..2079
                     /locus_tag="VFO72_06535"
     CDS             1414..2079
                     /locus_tag="VFO72_06535"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9478981.1"
                     /translation="MLRIGKNAKDQEDTDHSTHKPEVPDYSTPKTYSSYQGMGVEAKP
                     ATESAPTPPRGAMTESETLARDIKEGTLSGFVGGGTDVTGEANFKAMMRVDGHFSGRI
                     TSSSGTLIVGNNGKVDANIEVAVAVIHGTINGDIIATQRLELGRAAKVTGNIQTPSLV
                     IEQGAIFEGSCKMLQMHTAAEKAKKDTKVEVPLDTTKMPPPVRTDAASKPTTIPNVSN
                     VVS"
     gene            2158..4200
                     /locus_tag="VFO72_06540"
     CDS             2158..4200
                     /locus_tag="VFO72_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9478982.1"
                     /translation="MKRAAFVLVCSLLLLLVSVDLTLGQIPLRVLLQITKAEDERRWD
                     SELRDLLNHDNAAIRKRAALAAGRIGNEDSVPVLTKLLQDADGGVRSMAAFALGEVES
                     ANAADALIEIVKNTKERGELRARALEALGKIAAALPQEQQTRQRELGAVIREALEVES
                     TQPASQLTVLLGLTAALRSRPADAGPTIAKFLSSPDARVRSDAANALARLRLKNANDQ
                     LRKLLTDDPDPNVRANAARVLGATEDKTAFDALLTRTTSDPDSRVRVSSIRALAALKD
                     PRAAEPLMKLSAVDTNELLEIATTLGRLLPQKRDQTAIAWLRKLNETLKHTAPEVELA
                     FARIAPASYLSDLRRSMNVTDWRAAAAIANALGEIGAWPDSLKEQSDLWPSAETLTRK
                     LLAQPNIDYAIPDVLRALAAFKPKDLQQILVAHLNKPDVIVRATAADLLGDLPPSTEI
                     TQTLIAAWPRVSDDSLNDASLSILDALGKQKTVTANETIKKALESEDYLLRRRAVALL
                     KANGAGDFSSRIGTVKPKGTAQVYRRALSRIGRRVRAIVTTSKGSFTIELLPSEAPLT
                     VDNFVQLAQRGYFRGITIHRVVPNFVIQDGDPRGDGNGGPGYQIRCEINQVLYDRATL
                     GMALSGKDTGGSQWFVTHAPQPHLDGGYTVFGRVIAGMDVVDRTVRGDVVKSVVIR"
     gene            4227..5945
                     /locus_tag="VFO72_06545"
     CDS             4227..5945
                     /locus_tag="VFO72_06545"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET9478983.1"
                     /translation="MWLLCLVSGSAFAQSKVEPAERHHYTIDIKIDFDRLSYTGTETV
                     RWINHGDKSASVLYFHLYPNLRVGDQLLPANGTTAEADEPRIDILEVRTSSDNSALFS
                     ALDDQGTTLRVNLREQVAPGSSTDIVIKFKGSVPEIDRDETSLTTHIVKQVSAALRSD
                     RETRRARDINFRCRGVMLLGSAYPVLAVHDGDEWRRKVEPSVGDLLFNEAADYEVTVS
                     APSGVEVFTSGVETAARSDKNTQTFTATAMRDFAILAGRGLRSEHTEVNGVDVRSIYF
                     AEHERVGKRALVVAANALRTFTTLFGPLPFKQISIAEAPLVAGLGSCEFSGLNVIASA
                     FYVDFDSPAVRNLPEIIREQRPSVEESLEWTVAHLVAHQWWGAGVGNDPAREPILDEA
                     LSSWSSLLYYQRMYGPEKAAVVLDDQVRGVYRMYRTFGGDDMDANRPSREYRNTFQYA
                     AIVTAKGALMFVELQRSLGEEKVLAALRKYYQANLYEIAQLEDLRIALVAEAPVEQRR
                     MIGRTFTRWLTAKRGDEDIAPPDQELAATLGIQSKQAARSGDKNALTALGRVGRFFWQ
                     QMTRIR"
     gene            5956..6477
                     /locus_tag="VFO72_06550"
     CDS             5956..6477
                     /locus_tag="VFO72_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HET9478984.1"
                     /translation="MNLDAFPLERARSRESANVETSLERLSWLMDDLFRVPVLGWRFG
                     LDALIGLIPGFGDTATSLVSFYILAAGVRYRVPKITLLRMGLNIALDYVVGALPVVGD
                     LVDAWWKSNHKNLDLLKKRATVSTEEARSGRISDWLFVGVIILGLIALAAGSLFVSFY
                     LLLKLGELLAGLN"
     gene            complement(6604..>6890)
                     /locus_tag="VFO72_06555"
     CDS             complement(6604..>6890)
                     /locus_tag="VFO72_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478985.1"
                     /translation="IGIHMPRYESDTDVEAVRELIGKYGITEPVAVDNEHKLKDAFHN
                     HQGFVPAYYLFDAEGKLKSFAAGERGLDMLKSAIERLLPAEETIASGQVA"
BASE COUNT         1553 a         1967 c         1937 g         1433 t
ORIGIN      
        1 gtatccagcc gcgcatgcca gcgcttggcg gcattgcagt aaggacagcg aggatgtttc
       61 tcttcactgt cgaaaaactg gcgcacagct ttgggcgagg tcaactcctc gatagttgga
      121 ctgtagcgct tgatttccga gtacgcgaaa cgcggcgcga gtccgatctg ttttgtgaag
      181 atatcgaaaa tacaggcaag acaaacaagc gttgtgtatt ccgtgacggt tccgggagga
      241 aagtcactga aatcgagttc cgtcaacttc atggctaact gcttactgtg accaacgccg
      301 gtcggagatc ttcgtcggtg tgaccgacgg caagatccaa ctctgcttcg acatttccgg
      361 tggctgaggc gagcgtttgg aagtcaaaga gccggtgatc cagcaggtgc gtcggtacaa
      421 cattactgag ggcgttgagc attgaagcgc gaacgtgagg tatttcttcc tcaaggtgac
      481 taacaagctt cttcatgtgc acacgcttca ggtttgactg ggagccacag agatcgcacg
      541 ggatgatcgg gaaatcgcat cgcgacgcgt agtcagcgat gtctcgttcc gagcagtaag
      601 ccagcggtcg aataacggta ttgcggccgt cgttcgaccg aagcttcggc ggcatcgcct
      661 tcagtgagcc gacgtagaaa aggttgagta agagagtcgt gatgatgtcg tcggcgtgat
      721 ggccgagtgc gattttgtta caaccctcct cgaccgcgat gttgtaaaga acgcctcggc
      781 gcagccggga acagacagag cagtatgttt tgccttcggg aacgagcctt ttgacgatcg
      841 aataggtgtc gcgctcgacg atccggaagg gaacgtcgag tttactgagg tactcgggca
      901 tcacttccgc gggaaatccg ggctgttttt ggtcaagatt gacggccaga aggtcgaatt
      961 tgacgggcgc ccttcgccgg atatcgagaa gcaggtcaag cattgtgtgg ctgtccttgc
     1021 ccccagaaag acagaccatc acacggtcgc cgtcctcgat catcgcaaaa tcagagatcg
     1081 cttcgcctac gcggcgcagc agccgggtct ttgttttatc gtcacaacgc atggctgagc
     1141 cccgccagaa tgccatgtcg cgtgcctcgg cgcaaatcag cgccggacgc tacacgtaaa
     1201 acacttgtcg ttgcattttc gttataaggc tagacaccag tggccctgtt tgtctatgat
     1261 gatgagcccg ctttcgggcg gcgaaccatt tttcgacctg gctgaccaag tcaggaaatc
     1321 cacaaacaaa aggaaagcgc gtcgtttcgt tgcttcaagg ttccgacggc cggtgactaa
     1381 acttccgcgc ccccatattt acggaggtaa caaatgctta ggataggaaa gaatgccaag
     1441 gatcaggaag atacggacca ctccacacac aaaccggaag ttcctgatta ctcaactccc
     1501 aaaacttact cctcctatca gggaatgggt gttgaggcaa aaccggcaac ggaatcggcg
     1561 ccaaccccgc ctcgcggcgc catgaccgag agcgaaacgc tcgcacgcga tatcaaagaa
     1621 ggcacactca gcggattcgt gggtggcggc accgacgtca ccggcgaagc gaattttaag
     1681 gcgatgatga gagtcgacgg ccacttttca ggccggatca cttcatcgag cggcacgctc
     1741 atcgttggca acaacggtaa agtcgacgca aacattgaag ttgccgtcgc tgtcattcac
     1801 ggcaccatca acggcgacat tattgccacg cagcggctgg aactcggccg cgccgcgaaa
     1861 gtcaccggca acatccaaac tccgtcactc gtgatcgagc aaggcgcgat cttcgaaggt
     1921 agctgcaaga tgcttcagat gcacacggcc gccgagaaag caaagaaaga cacgaaggtg
     1981 gaggtaccac tcgacaccac gaagatgccg cctccggtcc gtaccgacgc ggcgtccaag
     2041 ccaaccacca tcccgaacgt ctcgaacgtc gtcagctaac cacttcgcat attgactatc
     2101 cacagattac gcagatttaa tctgtgtaat ctgtggattc ttgtttttag gagccaaatg
     2161 aaaagagcag ctttcgtcct ggtttgcagc cttctgctgc tattggtttc cgtcgatttg
     2221 actctcggcc agattcccct tcgggttctt cttcaaatca caaaggcgga agacgagcgg
     2281 cgttgggaca gtgagctgcg cgatctgctg aaccatgaca acgcggccat tcgcaaacgg
     2341 gcggcactcg cggccggacg gatcggaaac gaagattcgg tgccggtgct tacgaaactg
     2401 ctgcaagacg cagatggcgg tgtgcgctcg atggccgctt ttgcgctcgg agaagttgag
     2461 tcggcgaatg ccgcggatgc tctgatcgaa atcgtgaaga acactaagga gcgcggcgaa
     2521 ctcagagcgc gtgccttgga agcgctcggc aagatcgccg ctgctttgcc acaagaacag
     2581 caaacacgtc agcgtgaact cggagcggtg attcgtgaag cgctggaggt tgaatcaacg
     2641 cagccggcga gtcagttaac tgttctactc ggtctgaccg ccgcactgag atcccggccg
     2701 gcagatgcgg gtccaaccat cgcgaagttc ttgagttctc cagacgcccg cgtccgctcg
     2761 gatgccgcaa atgcgcttgc acgcctgcgt ttgaagaacg ccaatgatca acttcgcaaa
     2821 ctgctcactg acgatccgga tccaaacgtt cgtgcgaatg ccgcgcgcgt gcttggcgcc
     2881 accgaggata aaactgcgtt cgatgcattg ttgacacgca ccacgtcgga tccagattca
     2941 cgcgtcagag tcagcagcat ccgcgcactt gcggcgctca aagatcctcg cgccgccgaa
     3001 ccgttaatga agctctccgc agtcgataca aacgaactgc tcgagatcgc cacgacacta
     3061 ggccgcttac tcccgcaaaa acgcgatcag acggcaattg cctggttgcg caagctcaac
     3121 gaaacactga aacacacggc gccggaagtc gaactcgcgt tcgcgcgcat cgcgccggcc
     3181 tcgtatctct cagacctgcg ccgttccatg aatgtcactg actggcgtgc cgctgccgcg
     3241 attgcaaacg cactcggcga gattggagcc tggccggatt ctctcaaaga gcaatccgat
     3301 ttgtggccgt ccgctgaaac gctgacacgc aagctcctcg ctcagccaaa catcgactac
     3361 gcaattccgg atgtcctgcg agcgctcgcc gctttcaaac caaaagatct ccaacagatt
     3421 ctggtggcac atctaaacaa acctgatgtg atcgttcgcg ccaccgccgc agatctgctc
     3481 ggcgacttac cgccttcaac agagattacg cagacgctga tcgccgcctg gccgcgtgta
     3541 tccgacgact cgctaaatga cgcgtcgctg tcgatcctcg acgcgctcgg taaacaaaag
     3601 acggtaacag cgaacgaaac tatcaagaaa gcgctcgagt cggaggatta tcttcttcgc
     3661 cgccgggcgg tcgctttgct gaaagcaaat ggcgcgggcg acttctcgtc tcgcatcgga
     3721 acagttaagc cgaaaggcac tgcacaggtt taccgccgcg ccctctcgcg catcggccgg
     3781 cgtgtgcgtg ccatcgtgac taccagcaaa ggttcgttta ctattgagct actccccagc
     3841 gaagcgcccc tgacggtaga caacttcgtt cagcttgccc aacgaggcta ttttcgcggc
     3901 atcactattc accgggttgt acccaatttc gtgattcagg acggcgaccc gcgcggcgac
     3961 ggtaatggcg ggcccggata ccagatccgc tgcgaaataa accaggtgct ttacgaccgc
     4021 gcgacgttgg ggatggcgct ttcaggcaag gacacaggcg gctcgcaatg gtttgtaacg
     4081 cacgcgccgc agccacatct tgacggcggc tacactgtgt ttggtcgcgt gatagccggc
     4141 atggacgtgg tcgatcggac agtgcgcggc gacgtcgtga agtcagttgt aattaggtga
     4201 caaagacaca aagaacatta ataaccttgt ggctcctgtg cctcgtcagt ggctcagcgt
     4261 tcgcacaatc gaaagttgaa ccggcagagc gacaccacta cacgatcgat atcaaaatcg
     4321 atttcgatcg tctttcctac acgggcacgg aaacagttcg ctggatcaat cacggtgata
     4381 aatctgcctc cgtcctctac tttcaccttt acccaaacct gcgcgtcggc gatcaactgc
     4441 tgccggcgaa cggcacgacc gccgaagccg acgagccgcg catcgatatt ctcgaagtgc
     4501 gcacgtcgag cgataactcc gcactctttt ctgcgcttga cgatcagggc accacactgc
     4561 gcgtgaacct gcgcgaacag gtcgcgccgg gaagctcgac tgatatcgtt atcaagttca
     4621 aaggttcggt tccggaaatc gaccgcgatg aaacgagtct aaccactcac atcgtgaagc
     4681 aagtcagcgc cgcgttgcgt agcgaccgcg aaacacgacg cgcgcgcgat atcaacttcc
     4741 gctgccgcgg cgtcatgcta ctgggaagtg cttacccagt gctggccgtt cacgatggcg
     4801 acgagtggcg acgcaaagtc gaaccctctg tcggcgactt gctcttcaac gaagcggcgg
     4861 attacgaagt gacggtcagc gcgccatccg gcgttgaagt cttcacttcc ggcgtagaaa
     4921 ccgcagcacg ctccgacaaa aatacgcaga cattcaccgc aactgcgatg cgcgacttcg
     4981 cgattcttgc cggtcgcggg ttgcgcagcg aacacaccga agtgaatggc gtcgacgtcc
     5041 gttcgatcta tttcgccgaa catgaacgcg tcggcaaacg cgctctggta gtcgcagcta
     5101 atgcattgcg aaccttcacc acactgtttg gccccttgcc gttcaaacag atcagcattg
     5161 ccgaagcgcc gcttgtcgcg ggccttggga gttgtgagtt ctccgggttg aatgtcatcg
     5221 ccagcgcctt ctacgtcgat ttcgattcgc ccgcggtgcg caatcttccc gagatcattc
     5281 gcgaacaacg gccgtcggtt gaagagagtc tcgaatggac ggtggcccat ctcgttgccc
     5341 atcaatggtg gggtgcagga gtcggaaatg accccgcgcg cgagccgata ctcgacgaag
     5401 cactctcaag ctggtcgtcg ctgctctact accaacggat gtacggacca gaaaaagccg
     5461 ccgtggtgtt ggacgatcag gtgcgcggtg tgtatcgcat gtatcgaacg tttggcggcg
     5521 acgacatgga tgcgaaccgg ccatcgcggg agtatcgcaa cacgtttcag tatgccgcga
     5581 tcgtcaccgc gaaaggcgcg ttgatgtttg tcgaactcca gcgttcgctt ggtgaagaga
     5641 aagtcctcgc cgcgttgcgc aagtattacc aggcaaatct ctacgagatc gcgcaactcg
     5701 aggacctgcg aatcgcgctt gttgccgaag cgccggtcga acaaaggcga atgatcggac
     5761 gtacatttac ccgctggctc accgccaagc gaggtgacga agacatcgcg ccgccggatc
     5821 aggagttagc cgcaacgctt ggcattcagt cgaaacaagc tgcgcgttcc ggcgacaaga
     5881 atgctttgac ggcgctggga agagtcggaa gatttttttg gcaacagatg acacgtattc
     5941 gctgagatga gagctttgaa tttggacgca tttccgctgg aacgagcccg gagccgcgag
     6001 tcggccaacg ttgagacgag tctcgaacgg ctgagctggc tcatggacga tctgtttcgt
     6061 gtgccggtgc ttggttggcg ctttggcctc gatgcgctga tcggactgat tccgggtttc
     6121 ggtgatacgg cgacttcgct cgtctctttc tacatccttg ccgccggcgt ccgctatcgc
     6181 gtgccgaaga tcacgctact cagaatggga ctgaacatcg cgctcgatta cgtcgtcggt
     6241 gcgctgccgg tggttggcga cttggtcgac gcctggtgga agtcgaacca caagaacctc
     6301 gacctgctga aaaaacgagc gaccgtgtcc actgaagagg cacggtcggg aagaattagc
     6361 gactggttgt ttgtcggagt tattattctc ggtttgattg cactcgccgc cggcagtctg
     6421 ttcgtcagtt tttatctctt gctcaagctc ggagaactcc tcgccggtct aaactaacac
     6481 gcaatctgaa cccgctcttt cctgggaccg caggcgtctc gcttgcatgg cagcgacgga
     6541 tccacttgcg aaacactgca ggcgagacgc ctgcggtccc aggaaagagc gggttcaaaa
     6601 gatctaggcg acctgacccg aggcaatggt ttcttcagcc ggtaacagac gttcgatcgc
     6661 cgacttcaac atgtctaagc cgcgctcgcc ggcggcaaag cttttcaact taccttctgc
     6721 gtcaaacagg taatacgccg gcacaaaacc ctggtggttg tgaaacgcgt ctttgagttt
     6781 gtgttcgttg tcgaccgcga ccggctcggt gattccgtat ttgccaatca actctcgcac
     6841 tgcctcgacg tccgtatcgc tttcgtaacg cggcatgtga ataccgatca
//
LOCUS       DASTEY010000376         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_914917, whole genome shotgun sequence.
ACCESSION   DASTEY010000376 DASTEY010000000
VERSION     DASTEY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914917"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2367
                     /locus_tag="VFO72_06560"
     CDS             <1..2367
                     /locus_tag="VFO72_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478986.1"
                     /translation="GFTAVAVLTLALGIGANTAIFSVVNAVLLRPLQFRDPGRLVMIW
                     EDATFAGFPRNTPAPANYVDWKTQTQSFEDVAASAESTFNLTGDGDPERVNAYKVTAN
                     FFPLFGVAPALGRTFVADEDRPGAHKVAVLSHGLWQARYGGDPQIVNRDIQLDGEKYT
                     VAGVMPAGFQFFESDVRLWVPLELDAEELANRGGHYLKVVARLKPGVTLDQAQADLSA
                     VMARIAKDHPSETFEGKLGAFAMPLREQLTSDVRGSLVVLLVAVAFVLLIACANVAGL
                     LLARAVARRREIALRLALGASRLRVVRQLLTESLLLAAAAGIIGSILAYWSFAFLQRL
                     IPAEMALLTSLTLDTRILLFTLLISLLTGVVFGLVPALQSAKVDLNEALKQSTRVTSA
                     GNLRSALIVFEVALSLVLLVGAGLLIQTLFQLFNQYSVLEPEKVLTMRTVLPRSKYRE
                     PQQRHAFYQQVLDRVEHVPGVISAGYTTSVPLLWKGGTSGFVPEKITSPIPGMAYDAN
                     HREVSADYLQTMKIPLRQGRYFGERDNETSMRVAIINETMARQYWPGENALGRRFNIG
                     DPNDGEWMEIVGIVADVRQMGLDQPVKAEMYVPYQQVTDWPGYVPRELAIRTTGDPSN
                     IVGSVRQIIREVDPDQPVSNIATMAEVLSVEAEERRMGMIMLVAFAGLALLLASLGIY
                     GVLAYFVTQHTNEIGVRIALGANRRNILALVLKKGMGLTLLGVAIGLAASFALTRLMS
                     SLLFGVKASDPLTFFVVPLLLATVALVACWIPARRATRVDPMVALRYE"
     gene            2505..>3572
                     /locus_tag="VFO72_06565"
     CDS             2505..>3572
                     /locus_tag="VFO72_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478987.1"
                     /translation="MDPLLKDLRYGLRMLAKNPGVSAIAVLTLALGIGANTAIFSVVS
                     AVLLNPLPYKDPDRLVSIWENVPEHGRWRAAPANFFDWKKQNTVFENVAAFGGSTMTL
                     TGDGEPQQLMGTRVSDGYFAVVGATPMLGRAFLPEEHEPGKDRVVILGHSLWQSRYGA
                     DQHILTRTVTLDGNSYTVVGVMPPGLYPVRPTTAGRIEFDEQLQDYWHPYAFTAQFAA
                     YRSAHVLGVVARLKPGVTIAQAQAEMDTIGARLAQEHAANKDEGIIVNQFLNEVVGNV
                     RPALLTLLVAVGLVLLIACANIAGLLLAQHAARTKEIAIRAALGAGRLRLVRQFFAEG
                     LLLSLFGTVAGLLLAQVGLQVL"
BASE COUNT          692 a          973 c         1105 g          802 t
ORIGIN      
        1 ggttttaccg ctgttgccgt gctcacgctc gcgttgggaa ttggcgccaa tacagcgatc
       61 ttcagtgttg ttaacgcagt cctgctcagg ccgctgcagt ttcgcgatcc cggacggctg
      121 gtgatgatct gggaggacgc gacgtttgcc ggttttccgc gaaacacgcc cgcgcccgca
      181 aattacgtag actggaaaac acagacgcag tcgttcgaag acgtcgcagc aagcgccgag
      241 tcgacgttca atctaaccgg cgacggtgat cccgaacgcg tcaatgcgta caaggtcacc
      301 gcaaactttt ttccgctgtt tggtgtcgcg ccggctctcg ggcgcacgtt tgttgccgat
      361 gaagatcgcc cgggcgcgca taaggtcgcg gtgctgagtc acggactctg gcaggcgcga
      421 tatggcggcg atccacagat tgtcaatcgc gacattcagc tcgatggcga gaagtacacc
      481 gtcgccggcg tcatgcccgc gggctttcag ttttttgaaa gcgacgtgcg gttgtgggtg
      541 cctttggagc ttgatgcgga agaacttgcg aatcgcggtg gtcattatct gaaagttgtt
      601 gcgcgtttga agccgggcgt cacattggac caggcacagg cggacctctc ggctgtgatg
      661 gcgcgcattg cgaaagacca tccgtccgaa acgttcgaag gaaagttagg cgcgttcgcg
      721 atgccactgc gcgaacagtt gacgagtgat gttcgtggtt cgttggtggt gttgctggtg
      781 gccgtggctt tcgtgctgtt gatcgcttgc gcgaacgtcg cgggattgtt gctcgcccgc
      841 gcggtagccc ggcgacgtga aattgcattg cgtctcgcgt tgggtgcgag ccgtttgcgc
      901 gtcgtcagac agttactcac tgagagtctg ttgctggcgg cggcggcggg aataatcgga
      961 tcgatcctgg cctactggag tttcgcattt cttcagagac tcattcccgc cgagatggcc
     1021 ctgttgacca gcctgacact ggacacacgc atcctgctgt tcaccctttt gatttcgcta
     1081 ctgacgggag tcgtgttcgg ccttgtgccc gcgctccaat cggcgaaggt cgacttaaac
     1141 gaagcgttga agcagagtac gcgtgttacg tctgccggca acttgcgcag cgcgttgatt
     1201 gtcttcgagg ttgcgttgtc actcgtgctg ctcgtcggcg cgggactgtt gattcaaacg
     1261 ttattccaac tcttcaacca gtactcggtg ctcgaaccgg aaaaggttct gacgatgcgc
     1321 accgtgttgc cgcgatcgaa atacagagag ccacagcagc gtcacgcctt ttatcaacag
     1381 gtgctcgatc gcgtggagca cgtgccggga gtgatctccg ccggctacac cacgtcggtg
     1441 ccgctgttgt ggaagggcgg cacgagcggt tttgttcccg aaaagataac gtcgccgatt
     1501 cccggcatgg catacgatgc gaatcatcgc gaagtcagcg ccgattacct ccagacgatg
     1561 aagatcccgc tgcggcaggg gcgttacttc ggcgaacgcg acaacgaaac gtcgatgcgt
     1621 gtggcaatca ttaacgaaac gatggcgcgg cagtactggc ccggtgaaaa cgcgctggga
     1681 cgtcgcttca atatcggcga tccgaatgat ggtgaatgga tggagatcgt cggcattgtc
     1741 gccgacgtcc ggcagatggg actcgaccaa cccgtcaaag ccgaaatgta cgtgccatac
     1801 cagcaggtca ccgactggcc cgggtacgtt ccacgcgagc tcgcgattcg caccacgggc
     1861 gatccttcga acatcgtagg ttcagtgcgc cagatcattc gtgaagtcga tccggatcag
     1921 ccggtctcaa acattgccac gatggctgaa gtgctgagcg tcgaagccga agagcgacgc
     1981 atggggatga tcatgctggt ggcgtttgcc ggattggcgt tgctgctcgc atcacttggc
     2041 atttatggag tgctcgcgta tttcgtcacg caacacacaa atgagatcgg cgtgcggatc
     2101 gctctgggcg cgaaccggcg caatattctc gcgctggtgt tgaagaaggg aatgggtctg
     2161 acgctgctcg gcgttgcgat cggtctcgct gcttcattcg cgttgacgcg cttgatgagt
     2221 tcgttgttgt tcggtgtcaa agcttctgat ccgttgacgt tcttcgtcgt gccgttgctg
     2281 ctcgccactg tagcgctcgt ggcgtgttgg attccggcgc ggcgtgccac acgagtggat
     2341 ccgatggtgg cgctcaggta cgagtgacga gattgtcgat tgccgattgt cgattgccga
     2401 ttttttgaaa ccagttggac ggggcaatca acaaccaaca atcaacaatc gaaaatcgaa
     2461 aatcgaaaat caaaaatcga aaatcaaaaa tcgaaaatgt cgttatggac cctctactca
     2521 aagaccttcg ttacggcctg cggatgttgg cgaagaaccc gggcgtgtcg gctattgccg
     2581 tactcacgct cgccctgggc atcggcgcga acaccgcgat cttcagcgtt gtcagcgcgg
     2641 tgttgttgaa tccgttgccg tacaaagatc ccgatcgact cgtttcgatc tgggaaaacg
     2701 ttccggagca cggccgctgg cgtgccgcgc ccgctaactt cttcgactgg aaaaagcaga
     2761 acaccgtttt cgaaaacgtc gctgcgtttg gtggttcgac gatgacgttg accggtgacg
     2821 gcgaaccgca gcaattgatg ggcacgagag tcagcgacgg ttattttgcc gtcgttggcg
     2881 ccacgccaat gctcggccgt gcattcctgc cggaagagca cgaacccggc aaggaccgcg
     2941 tcgtcattct gggccattct ttgtggcaga gtcgctacgg cgcggaccag cacatcctaa
     3001 ctcgaacagt cactctcgac ggtaacagct acacagtcgt cggcgtgatg ccgcccgggc
     3061 tttatcctgt tcggccgacc accgcgggcc gcatcgagtt tgacgaacaa ctccaggact
     3121 actggcatcc atacgccttt accgcgcagt tcgctgcgta tcgatctgct cacgtgctgg
     3181 gcgtcgtcgc gagactgaag ccgggtgtga cgatcgcgca ggcgcaggcg gagatggaca
     3241 cgattggcgc tcgtctcgcg caggaacacg cggcgaataa agatgaggga atcatcgtca
     3301 accagtttct gaatgaggtg gttggcaacg tcagaccggc actgctcacg ttgctcgtcg
     3361 cggtcgggct ggtgttgctg attgcgtgcg caaacatcgc gggactgtta ctggcccaac
     3421 acgcggcacg cacgaaagag attgcgatcc gtgcagcgct gggcgccggc cgtttgcgcc
     3481 tggtgcgaca gtttttcgcc gaaggactgt tgctttcgtt gtttgggacc gttgccgggc
     3541 tgttgctggc ccaggtgggt ctacaggtat tg
//
LOCUS       DASTEY010000377         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1425874, whole genome shotgun sequence.
ACCESSION   DASTEY010000377 DASTEY010000000
VERSION     DASTEY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425874"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFO72_06570"
     CDS             complement(<1..910)
                     /locus_tag="VFO72_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HET9478988.1"
                     /translation="MYILGINAYHGDASAAIIKDGRLIAAVEEERFNRIKHCAGFPER
                     SIRYCLDAAGVRLEDVDQIGISRDPAAHLHKKILYAASRFGVFRQAKDRLANAAKTRD
                     LKDELTRVFSISKRSLRAQFHNVEHHLAHLASCFFVSPFERAALLSIDGFGDFISTMW
                     AIGEGNSIEVLGQVEYPHSTGIVYTATTQFLGFPHYGDEGKVMGLAPYGRPRFINEFR
                     KIIRTEERGRFRLNLDYFRHHAEGVEMTWDNGSPVIGQIFSEEFVRTFGAARTPGAPL
                     TDRDRDIAASLQLRLEEIAFHVLNHLH"
     gene            complement(841..1668)
                     /locus_tag="VFO72_06575"
     CDS             complement(841..1668)
                     /locus_tag="VFO72_06575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9478989.1"
                     /translation="MLEHITPLILTYNEAPNIGRTLERLTWARDIVVVDSFSNDETIE
                     IATSFSQVRVFPREFDCHRNQWEFGLSETGISTPWVLALDADYIVTSEALAELKNLRP
                     DAATAGYRAKFTYCINGSRLHSGIYPPTTVLYRRKVADYVQDGHTQRVVVDGRVEELR
                     STLLHDDRKPLSRWLRSQVRYAELESKKLLTADRKSLDIPDRLRRWSILAPPAALFYC
                     LIVRGGIFDGWPGLYYAFQRTVAELLLSLYLIESRCTFSASTRITAMLQRQSSKTAD"
     gene            complement(1673..>2736)
                     /locus_tag="VFO72_06580"
     CDS             complement(1673..>2736)
                     /locus_tag="VFO72_06580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9478990.1"
                     /translation="AIVPMCRALRERGIDVFLATTSHGLARMETDCMTHFTGVPARLF
                     PAQLGESFKYSRPLAKWLKASVRDFDLVHIHAVFNHASIAAAAACRQSGVPYVVRPLG
                     TLDPWSMKQKGGRKRIFWWLEGKRMFERSAAVHYTAAGEKTATEEYLGLNHGRVIALG
                     VEVNGTTPMKKAAEWFPGLTGHPYVLALSRLDPKKAVDVLIEAFISLDRDDWRLVVAG
                     DGPRDYVSSLKQKARGSGKIIFTGWVEGEVKQALLREASLLALPSRHENFGLSVMEAM
                     ARGVPALVTPEVNLADEIEAAGAGWTVDLSRLQNGLAAVFNGNIERMRRGRAAHQFAQ
                     QYSWQRTATELAELYRQIA"
BASE COUNT          599 a          826 c          711 g          600 t
ORIGIN      
        1 cgtgaagatg attgaggacg tgaaacgcga tctcttcgag gcgtaattgc aaactcgccg
       61 caatgtcgcg gtcgcgatca gtcagcggcg cgcccggtgt tcgtgcagcg ccaaacgttc
      121 tcacaaattc ctcggaaaat atctgcccga ttaccggcga gccgttgtcc cacgtcatct
      181 cgactccttc ggcgtggtgc cgaaagtagt cgagattcaa acgaaaacgg ccgcgctctt
      241 cggtgcgaat aattttccga aactcgttga tgaatcgcgg ccggccataa ggcgccagtc
      301 ccatcacttt tccttcgtca ccgtaatgcg gaaacccgag aaactgcgtg gtggcggtgt
      361 aaacgatccc cgtcgagtgt gggtattcaa cctggcccaa cacttcgatt gaattgcctt
      421 cgccgatggc ccacattgtg gagataaagt ctccaaagcc gtcgatcgac aacagtgccg
      481 cacgttcgaa tggcgagaca aagaaacagc tcgcgagatg cgccaggtga tgttcgacgt
      541 tgtgaaactg cgcccgcaga ctccgctttg aaatactgaa aacacgagtt agctcgtctt
      601 ttaggtctcg tgtttttgcc gcattcgcga gtcgatcctt agcctggcgg aacacaccga
      661 atcgcgacgc tgcatacaag atcttcttgt gcaagtgcgc cgcagggtcg cgagagatgc
      721 cgatctgatc cacgtcttcc agccgcacgc ctgccgcatc cagacagtaa cgaatcgagc
      781 gctcgggaaa acccgcgcag tgtttgattc gattaaatcg ctcttcttcg accgcggcga
      841 tcagtcggcc gtctttgatg attgccgctg aagcatcgcc gtgatacgcg ttgatgccga
      901 gaatgtacat cgcgattcga tcaggtaaag cgagagcaat agttccgcca ctgttctttg
      961 gaaggcgtaa taaagtccgg gccagccgtc gaaaattccg ccgcggacaa tcaggcaata
     1021 gaacaacgcc gcaggcggag ccaggatgga ccagcgccgt agccgatctg gaatgtccag
     1081 agacttccga tcggcggtga gcagtttttt ggactcgagt tctgcgtagc gcacttgcga
     1141 acgaagccag cgactcagcg gttttcgatc gtcatgcagc aacgtggagc gcaactcttc
     1201 cactcgtcca tccacgacaa cacgctgtgt gtgtccatcc tgtacgtaat cagccacctt
     1261 tcgtcgatac aaaactgtgg tcggcggata gatcccgctg tgcaacctgc tgccgttgat
     1321 gcagtacgta aacttcgcgc gatagcccgc ggttgctgcg tcgggtcgga ggtttttcag
     1381 ttcggcgaga gcctcgctcg tgacgatgta atccgcgtca agcgcgagta cccacggagt
     1441 actgattccg gtttcgctca gtccaaactc ccactgattg cggtggcaat caaactcacg
     1501 cggaaacacg cgcacctgcg aaaacgatgt tgcgatctcg atcgtctcat cattgctaaa
     1561 gctgtcgaca acaacgatgt ctcgcgccca ggtaagccgt tccaacgtgc gtccgatgtt
     1621 tggcgcttcg ttgtaggtca gaatcagtgg cgtgatgtgt tcgagcatcc gttcaggcta
     1681 tctgccggta aagttccgcc aactccgtcg ccgtcctttg ccacgaatac tgttgcgcga
     1741 attgatgcgc tgccctgcct cgacgcatgc gttcgatgtt gccgttaaac accgcggcca
     1801 atccgttctg caaccggctc agatctacgg tccaacctgc gcctgctgct tcgatctcgt
     1861 ctgccaggtt cacttccgga gtgaccaatg ccggcacgcc acgcgccatt gcttccatca
     1921 cagataaacc aaaattctcg tgacgagacg gcagtgccag caaagacgcc tcgcgcaaca
     1981 acgcctgttt aacttcaccc tcgacccagc cggtgaagat gatctttccc gaaccacggg
     2041 ccttctgttt gagcgacgag acataatccc gtggtccatc acccgccacg acgaggcgcc
     2101 agtcgtcacg gtccagtgaa ataaatgcct cgatcaagac atccacagcc tttttgggat
     2161 ccagccgcga aagtgccagc acgtacggat gtccggtgag cccgggaaac cattctgcag
     2221 cttttttcat cggcgttgtt ccgtttacct ctaccccgag cgcgatgacc cgcccatgat
     2281 ttaatccgag gtactcttcc gtggcggtct tctcgccggc ggctgtgtaa tgcacggccg
     2341 cagacctttc gaacatcctc tttccctcta accaccaaaa tattctcttt cgcccgcctt
     2401 tctgtttcat gctccacgga tcgagcgtgc ccagcggccg cacgacataa gggacgccgg
     2461 actgccggca cgccgcggca gccgcgatag acgcgtgatt gaaaacagcg tgaatatgga
     2521 ccagatcaaa atcgcgcaca ctcgctttca accactttgc cagtggccgc gagtatttga
     2581 acgactcacc gagttgcgcg ggaaacaacc ttgccggcac acccgtgaaa tgagtcatac
     2641 aatccgtctc catccgtgct aatccgtggc tcgttgttgc cagaaaaaca tcaatccccc
     2701 gctctcgcag cgcacggcac atcggaacga tcgctg
//
LOCUS       DASTEY010000378         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1211455, whole genome shotgun sequence.
ACCESSION   DASTEY010000378 DASTEY010000000
VERSION     DASTEY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211455"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VFO72_06585"
     CDS             complement(<1..1420)
                     /locus_tag="VFO72_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478991.1"
                     /translation="MQITVLKFGSSVLRSESDLPTAVHEIYRWWREGFQVVAVVSAFG
                     NTTDDLTQRAHEVCDSPNDELVAALLATGEAASSALLGLALNRSGIRATVLDAQQAGL
                     ATGGPVLDANLTSINSASVLEALRNGVVVLPGFVGRSSGGKTTLLGRGGSDLTALFIA
                     HRLGAACRLIKDVDGLYTSDPNSTTGPSAARFGRVSYATAARVGGAVVQLKAIDFAEQ
                     HRLRFVVSSAGANRATEIGPYTDSVALSDPQVRPLRVALLGCGTVGGGVYQRLAALPE
                     LFDVVGVGARNIHQADVPLKLFTTHLEELIAEPVDVVIELLGGDEPARTVISKSLRLG
                     RHVVTANKSLLARDIATLQRLASHSGVALKYSAAVGGVLPALETVNRARNIAPLNSIS
                     GVLNGTTNFILDQLAKGTELANAVSAAQKSGYAERDSQLDLNGTDAAQKLILLARAAF
                     DVDLPLNSIEKRGIQDLNAKQVR"
     gene            complement(1504..>2542)
                     /locus_tag="VFO72_06590"
     CDS             complement(1504..>2542)
                     /locus_tag="VFO72_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9478992.1"
                     /translation="VGKDKGFTYSRSANPTVSALERRLAALEGADYCTCYATGLAAMT
                     VLCLALLRAGDHVVASEAVYGGTVRLFNQVLAPFGVTAEFVDTADEAALRKALTARPT
                     RFVFIETPANPTLKLTDIELTARIARDAGALLVVDNTLLTPALQRPFELQADVVLHST
                     TKFIEGHNATVGGALITRDPELHERFSFTRNAIGAIQSPFPAWLTLQGVKTIGLRMAQ
                     HSENALRIARFLETHPRVTKLLYPGLESFPQFELARKQQTSGGALIAFEIEGGVEAGI
                     RLMNNVRLCALAENLGSVETLITHPASMTHADVPVRQRQDAGITDGLVRLSVGLEDPV
                     DVIRDLLQALL"
BASE COUNT          553 a          746 c          703 g          540 t
ORIGIN      
        1 ctctaacctg ctttgcgttt aggtcctgga ttccgcgttt ctcgattgag tttaacggca
       61 gatccacatc aaaagctgcg cgcgccaaca agatcaactt ctgcgctgcg tctgttccgt
      121 tcagatcaag ttgtgaatcg cgctcagcgt agccactctt ctgtgccgca gacacggcat
      181 tggcgagctc cgtgcccttt gctaattgat cgagaatgaa attcgttgtt ccattcagca
      241 cgccggagat tgagtttaag ggagcaatat ttcgtgcacg attgaccgtt tcaagagctg
      301 gcagcacacc accaacagca gcgctgtact tcagtgccac gccgctgtgc gacgccaaac
      361 gttgtagcgt tgcgatatct cgagcgagta aagacttgtt ggctgtaact acatgtcgtc
      421 cgagtcgcag cgattttgaa atcacggtcc gcgccggctc gtcgccaccc agtaattcaa
      481 tcacgacgtc tacaggctcc gcgatcaact cttccaggtg tgttgtgaac aacttcagtg
      541 gaacatcagc ttgatgaata ttccgcgctc ctactccgac gacgtcaaat aactccggca
      601 acgctgcgag gcgctgatac actccgccgc caacggtgcc gcagccgagt aaggcaacgc
      661 gcaaaggtcg aacttgcggg tccgaaagcg cgacggaatc ggtgtacggt ccgatttcgg
      721 tcgcacggtt ggcgccggcg cttgagacga cgaatctcaa ccggtgttgt tctgcaaaat
      781 cgattgcttt gagttgcact accgcgccgc cgacccgcgc cgcggttgca taactcaccc
      841 gtccaaagcg cgccgctgat ggtccggtgg tggaattggg atcgctagtg tagagaccgt
      901 caacgtcttt gatgagccgg cacgccgcgc cgagacggtg cgcaatgaat agcgccgtta
      961 gatctgagcc acctcgaccc agcaacgtcg ttttgccgcc tgaacttcta ccaacaaaac
     1021 ccggcagaac gacaactcca tttcgcaaag cttcgagcac actcgctgag ttgatcgaag
     1081 tcaggttggc gtcgagcact ggtccaccgg tagcgagacc ggcttgctgc gcgtcaagca
     1141 cagtggctcg aattcccgaa cgattcagcg cgagtcccag taaagcggac gaagcagctt
     1201 cgcctgtggc taacagcgcc gcgaccagtt cgtcattcgg cgaatcgcac acctcgtgcg
     1261 cgcgttgtgt cagatcgtca gtcgtgttgc cgaatgccga cacgaccgcg acaacctgaa
     1321 agccttcacg ccaccaccga tagatctcgt ggaccgcagt cggtaaatcg ctttcgctcc
     1381 gcaacacaga gctgccgaac ttcaatacgg taatttgcat atttcctcct ttgtaggggg
     1441 tggccctccg tggccacccc accgcaccgg caataatcat ggggtggaca cggagggcca
     1501 cccctacaaa agggcttgca atagatcgcg aatcacgtca acgggatctt ctaaaccgac
     1561 ggagagtcgc accaggccgt cagtaatgcc tgcgtcctga cgttgcctga cggggacgtc
     1621 agcgtgcgtc atcgacgctg gatgcgtgat cagcgtttct accgaaccaa ggttctccgc
     1681 gagcgcgcag agcctgacgt tattcatcag gcgaatacca gcttcgactc ctccttcgat
     1741 ctcaaacgcg atcagcgctc ctccggaagt ttgctgcttg cgcgccagtt cgaattgcgg
     1801 gaacgactcc aagccgggat aaagcagttt cgtcactcgc ggatgtgttt ctaaaaagcg
     1861 cgcgattcgc agtgcgtttt ccgagtgctg cgccattcgc aagccgatag tctttacacc
     1921 ctgaagcgtc aaccatgcag gaaacggaga ttggattgcg ccgatcgcat tgcgcgtgaa
     1981 agagaaacgt tcgtgaagtt caggatccct cgttattaat gcgccgccaa cggtggcgtt
     2041 atgtccttcg atgaacttcg tagtcgagtg aagaacgacg tcggcttgca gctcgaatgg
     2101 tcgttgcaga gcgggtgtca acaaagtgtt gtcgacgaca agcagcgcgc cggcgtcgcg
     2161 cgcgattcgc gctgtcagct cgatgtccgt gagtttcaag gtcggattgg ctggtgtctc
     2221 aatgaagaca aaccgtgtgg gtcttgcggt caaggccttt cgcagggcag cttcatccgc
     2281 cgtgtcaaca aactctgccg tgactccaaa aggagcgagc acctgattga acagccgcac
     2341 ggtcccgccg tagacagctt cagaggcaac gacgtgatcg cccgcgcgca acagcgccag
     2401 acacaatacc gtcatcgccg ccagaccagt cgcgtaacac gtacagtaat cggcgccttc
     2461 gagtgcggct aagcgtcgct cgagcgcgga gacggtcgga tttgccgagc ggctgtaggt
     2521 gaaccctttg tctttaccga ct
//
LOCUS       DASTEY010000379         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1508211, whole genome shotgun sequence.
ACCESSION   DASTEY010000379 DASTEY010000000
VERSION     DASTEY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508211"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFO72_06595"
     CDS             <1..226
                     /locus_tag="VFO72_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9478993.1"
                     /translation="NLDSTNGEAVMELLRELHRGGATICMVTHDPRYAQHADRIVHLF
                     DGQIVEEEQGTERREIMERELKESGFGAIS"
     gene            223..891
                     /locus_tag="VFO72_06600"
     CDS             223..891
                     /locus_tag="VFO72_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9478994.1"
                     /translation="MISLRGVEKAFPHGPTKTYVLRRINLDIKPGEFVSIMGPSGAGK
                     STLLHILGMHDHSWTGEYYFDDVPVHQLDTKKRAALRNRNIGFVFQSYHLLDDQTVFE
                     NLELPLSYRDVSKKDRQSIVCDTLDRFQMVGKKDLFPTQLSGGQQQLVGIARAVVANP
                     RIILADEPTGNLHSAQGKEIMDMFKRLNDEGTTIVQVTHSEINAAYGNRIVQLEDGWI
                     VNGE"
     gene            997..3417
                     /locus_tag="VFO72_06605"
     CDS             997..3417
                     /locus_tag="VFO72_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478995.1"
                     /translation="MDAFLNDIRYAIRNLLKRPAFTLIAAVTLALGIGANTAIFSSVY
                     ALLLKPFPFPELDRVVAIWDSYPSRGVIRNEVAMANYLDWRAQNQSLEQLALYRWWSA
                     NLTGSDHPERIQGFQITANFLDALGVKPIMGRNFTEEENQPGKDAVVILTYALWQRRF
                     GGDPNIIGKKITLDSVAFTIVGVMPEEFRYPANAEVYSPIAITPRLASNRQFNSYYVV
                     GRVKPGVSIQSAQADIDNITARLEQQYPETNTGVRATVFPIVADTVRKYETGLWMGMA
                     AVAFVLLIACANVANLMLARASGRQKEIALRAALGASRWRIIRQLLTESSIVALFGGA
                     LGILVAVWGIDALRAANPGDAAKFAPGWHQLGINFTVLVFTIALSLFSGLLFGLAPAL
                     EVSKPNLNEALKEGNRQSSGRSHRLRNSLVVFEVAVALVLLVGAGLFFRSFLTLFRTD
                     PGFNPDNVLTMNLILSSAKYEDDTQSAAFYADLIQRVKAEPGVESAAAVNYLPLGGSN
                     SSDAYLVEGAPEPPPGEENISRYRVCTTNYFSTMQIPLLTGRAFTDQDKAGAPPVIIV
                     NETLARKHWPGQEAIGKRIRFDAPLDRAPWMEVVGVIKDVKHELTIDVTPEFYLPHAQ
                     DSWRSMVIVARTTVDPASLAGSIRQHVWAIDKDQPVFDVRTMQEVRSISVGLQKFNSL
                     MIGIFAAVALLLASIGIYGVMAFVVTQRTQEIGIRMALGARAPDVLKLVIANGMKLAA
                     LGLAIGLLASWVLTRFISKLLFGVEPTDPVTFGVVSLCLLAAAFLACYLPARRATKVD
                     PLEALRYE"
     gene            3516..>4521
                     /locus_tag="VFO72_06610"
     CDS             3516..>4521
                     /locus_tag="VFO72_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9478996.1"
                     /translation="METLFKDIRYGLRGLLKRKGFAAIAVLTLALGIGANTAIFTLVN
                     AVMLKSLPVSQPEQLVLFSDTTGEGTSYEDSPGSGHWLRFSYASYRYFHDHNQSFQDI
                     AALRSGVSRLSVRRAETQGAAATRASGQLVSGNYFSVLGAGAMRGRVLTPEDDKANAP
                     PAAVMSHRYWTEQFNSDPSVVGQTFVINGTNFTVVGITPTEFFGERVRRPPDFWLPLV
                     FHPQIELQQSFLEDNQAYWLMLLGRLKPGVQMEQAQITVNHALRQFLTEQAGSQLTED
                     RQRAIQNTYVTLVEGKGGISSLRRLYSKPLHMLMAVVGIVLLIACANVGSLLLARATA
                     R"
BASE COUNT          972 a         1256 c         1299 g          994 t
ORIGIN      
        1 aaacctcgac tcgaccaatg gtgaagcagt catggaactt ctgcgcgaac tgcatcgcgg
       61 cggcgccacg atctgcatgg tcacacacga cccgcgttat gcgcagcacg ccgatcggat
      121 cgttcatctc ttcgacggcc agatcgtgga agaagaacag ggcaccgagc gacgtgagat
      181 catggagcgc gagttgaaag aaagtgggtt cggagccatt tcatgatcag tcttcgggga
      241 gtcgaaaaag ctttccccca cggtcccacg aagacttacg tgctccgacg gattaacctc
      301 gacatcaagc cgggtgagtt tgtttcgatc atgggtccat caggagcagg gaagtcgaca
      361 ctgctccaca ttctgggaat gcacgatcat tcgtggaccg gcgagtatta cttcgacgac
      421 gttccggtcc atcaactcga caccaagaag cgcgcggcgc tgcgtaatcg aaacatcggg
      481 ttcgtgttcc aaagttatca cctgctggac gatcagaccg ttttcgagaa tctcgaattg
      541 ccgctctctt atcgcgacgt gagcaagaaa gatcggcagt cgatcgtttg cgacacactc
      601 gatcgttttc aaatggtggg taaaaaggat ctgtttccga cgcagttgtc gggtggtcaa
      661 caacagctcg tcggcatcgc gcgcgcagta gttgcgaatc cgcgaatcat cctggctgac
      721 gagccgaccg gcaacctgca ttcggcccag ggcaaagaga tcatggacat gttcaagcgc
      781 ctgaatgatg agggcacaac gattgttcag gtaacgcact ccgagatcaa tgcagcttac
      841 ggtaacagga tcgtccagct cgaagatggc tggatcgtca acggcgaata aattcgtgct
      901 gtccgtgtaa acacggattc acacagagct ataacccaaa gttcaaacct gtagttgctc
      961 agaacgtcac cagtgtgcct acgggcagga ggaactatgg acgccttcct gaacgatatc
     1021 cgttacgcca tccgcaacct ccttaaacgt ccggccttta ctctcatagc agccgtcaca
     1081 ctggctctcg gcatcggcgc caacactgcg atctttagtt ccgtgtacgc gctgttgctc
     1141 aagccgttcc catttcccga actcgaccgt gtcgtcgcca tctgggactc ctatccaagc
     1201 cgcggtgtga tccgtaacga agttgcgatg gccaactatc tcgactggcg agcgcagaac
     1261 caatcattgg aacagctcgc gctttaccgc tggtggagcg caaacctgac gggtagcgat
     1321 catcccgaac ggattcaggg ttttcagatc accgcaaatt ttttggacgc tcttggcgtc
     1381 aagcccatca tggggcggaa cttcacggaa gaagagaacc aaccgggcaa ggacgctgtc
     1441 gtcattctca cctacgcctt gtggcaacga cgcttcggcg gcgatccaaa catcatcggt
     1501 aaaaagatca cgctcgacag cgttgcattc accatcgtcg gcgtgatgcc ggaagaattc
     1561 aggtatcccg cgaacgccga ggtttacagt ccgatcgcga tcacaccgag actggcgagc
     1621 aatcgccagt tcaactcgta ctacgtggtc ggacgggtga agcccggagt gtcgatccaa
     1681 agcgcgcagg ccgacattga caacattacg gcacgactcg agcaacagta tcccgaaacc
     1741 aacaccggtg tccgcgcgac ggtctttccg atcgttgccg acacggtgcg caaatacgaa
     1801 accgggttat ggatgggaat ggctgcggtc gccttcgttc tgttaattgc atgcgcgaac
     1861 gtcgctaact taatgctcgc acgagcaagt ggacgacaga aagagatcgc gttgcgcgcc
     1921 gctttgggcg cgagccgctg gcgcatcatt cgtcaactgc taaccgagag ttcgatagtc
     1981 gcgctgtttg gtggcgcgct gggaattctt gtggctgtct ggggcatcga tgctttgcga
     2041 gcggcgaacc ctggtgacgc cgcaaagttt gcacccggat ggcaccagct cggaatcaac
     2101 tttacggtgc tggtctttac gatcgctctc tcacttttca gcggtctgct gttcggtttg
     2161 gcgccggccc tggaagtctc gaaaccgaat ctcaacgaag ctctgaagga aggtaaccgg
     2221 cagtcctccg gccgctcaca tcggctacga aattcactgg tcgtgtttga agttgctgtg
     2281 gctttagttc tgctcgtcgg cgccggtctt ttctttcgta gttttctcac gttgttcaga
     2341 acggatcccg gtttcaatcc ggataacgtg ttgacgatga atcttatctt gtcgtctgcg
     2401 aagtatgagg acgacacgca gagtgctgct ttttatgccg acctgattca gcgcgtgaaa
     2461 gcggagcccg gcgtcgaatc ggccgcggcc gtgaactatc tcccgctggg cggctcaaac
     2521 tcatcggatg cctatttggt tgaaggcgcg ccggagcccc cgccgggtga ggaaaacatc
     2581 agccggtatc gcgtgtgtac taccaattac ttctcgacga tgcagattcc cctcctcacg
     2641 ggccgagcat ttactgatca agataaagcc ggcgcgccgc ccgtcatcat cgtgaacgag
     2701 acactcgcgc ggaagcactg gcccgggcag gaggcgatcg ggaaacgcat tcgctttgac
     2761 gccccactcg atcgcgcgcc gtggatggag gttgtcggcg tcatcaaaga cgtcaagcac
     2821 gaacttacta tcgacgtgac gcccgaattc tatttgcctc acgcacagga cagttggagg
     2881 tcgatggtga tcgtggccag aacgacggtg gatccggcat cgctggcggg ttcgatccgc
     2941 cagcatgttt gggccatcga caaggatcag ccggtgtttg acgtgcgcac gatgcaggaa
     3001 gtgcgttcga tctcggtcgg actgcagaaa ttcaactcgc tgatgattgg aattttcgcg
     3061 gctgtcgctt tgttgctggc gtcaatcggg atctacggcg tgatggcgtt cgtcgtcacg
     3121 caacgcacgc aggagattgg gatccgcatg gctctgggcg cgcgcgcgcc ggacgtgttg
     3181 aagctggtga ttgcaaacgg gatgaagctg gccgccctcg gtttagcgat cggccttcta
     3241 gcatcgtggg tgttgactcg ctttatctcg aaattgctgt ttggagtgga gccaacagat
     3301 ccggtgacgt tcggtgttgt ttcgctgtgt ttgttagcgg cggcgtttct ggcgtgttac
     3361 ctgccggcgc ggcgcgcaac gaaagtcgat ccattggaag cactgaggta cgagtaaggc
     3421 aattgccaat tgcctattgc caatggccaa ttgctatttt gctcggtcgc ctggcgcaaa
     3481 tcggcaatcg gaaatcggaa attggaaata tcgagatgga aacattattt aaagatattc
     3541 gctacggttt gagaggactg ctcaagcgca aagggttcgc ggccatcgcc gtgttaacgc
     3601 ttgcactcgg catcggcgcg aacacggcca tcttcacgct ggtgaatgcc gtcatgctga
     3661 agtcgcttcc ggtcagtcaa ccggagcaac tcgttctgtt cagtgacacg acaggcgagg
     3721 gaacttcata cgaagacagt ccaggcagcg gtcactggct gcgtttctca tacgcgtcgt
     3781 atcgctactt ccacgatcac aaccaatcgt tccaggacat cgctgcgctg cgcagtggcg
     3841 tgagccgttt gagtgttcgt cgcgctgaga cgcagggcgc cgcggccacg cgcgcttccg
     3901 gccaactcgt ttccggtaac tacttttcag ttctcggcgc cggcgcgatg cgcggccgcg
     3961 tactcacacc tgaagacgac aaagctaatg cgccacccgc ggcggtgatg agtcaccgtt
     4021 actggacaga acagttcaac agcgatccct cggtagtggg ccagacgttt gtgatcaacg
     4081 gtacgaactt cactgtggtg ggcatcacgc cgacggagtt tttcggcgag cgtgtgcggc
     4141 gcccgcccga cttctggttg ccactcgtat ttcatccgca gatcgaactg cagcagtcgt
     4201 tccttgaaga caatcaggcg tactggttga tgttgctcgg gcgactaaaa ccgggcgtgc
     4261 aaatggaaca ggcgcagatc acggtcaatc acgcgctacg acagttctta actgagcagg
     4321 ccggcagcca gcttactgaa gaccggcaac gcgctatcca aaacacctac gtcacgctgg
     4381 tcgaaggcaa aggcggcatc tccagtctgc gaaggcttta ttcgaaacca ttgcacatgc
     4441 tgatggccgt tgtcggcatt gtgttgctga tcgcgtgtgc aaatgtcggc agcctgctgc
     4501 tggcccgggc caccgcgcgc a
//
LOCUS       DASTEY010000380         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_766690, whole genome shotgun sequence.
ACCESSION   DASTEY010000380 DASTEY010000000
VERSION     DASTEY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766690"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFO72_06615"
     CDS             complement(<1..1041)
                     /locus_tag="VFO72_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9478997.1"
                     /translation="MTRQRTSFTRVILGTLLVLAGLAAALVTVLARQSENITLAGAAA
                     ILSLVIALLLTIFIVPPLARSARVEVSNLDFPFEPTSGGGVFLVIIIVVGFAAWNTGN
                     NLLFLVFSLLCSTLFVGWIAARTSLRDLTVSARFPDHIFAAEPAPVIVTVRNTKRLLP
                     SFSVFVEARGPSSEPGGRRRYAKRLLAYFSYVPHHASAEQRVEQLFPKRGHVLIDGFE
                     LSTRFPFGFFRRRRRLRARNVDIIVYPKPEVISDQLHLLPMYAGRMPAVRRGAGHDLF
                     SLRDYQPQDDLRHIDWKSTARSRRLTVREFTSEDERRITIVLDTRLPGEESEELRQRF
                     ERGVVQTASLLKH"
     gene            complement(1166..2149)
                     /locus_tag="VFO72_06620"
     CDS             complement(1166..2149)
                     /locus_tag="VFO72_06620"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9478998.1"
                     /translation="MRNLETIKGGQVPSAFAIDQIERLQRAVETVIKGKPEAIRLAIV
                     TLIAGGHLLVEDVPGVGKTTLAHALARALDCNFQRIQFTSDLLPSDVIGLSVYNQHSG
                     VFEWKPGPVFANVVLADEINRTTPKTQSALLEAMAERHVTVEGATHHLPAPFIVLATQ
                     NPIEHHGTYPLPESQLDRFMLRLRIGYPAPADEQLILRDREYADPLEELEPVMSAHAV
                     VELQRAVTTVSVDDALVQYLMRIVAATRESEMLDLGVSPRGTLALFRAAQALALSEDR
                     TYCIPDDIKRLVVPVFAHRIVVSSRFSSSLRRSEEAEAVLTEILKTISVPL"
     gene            2340..2843
                     /locus_tag="VFO72_06625"
     CDS             2340..2843
                     /locus_tag="VFO72_06625"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9478999.1"
                     /translation="MEPLNDNQKTRRDTRNQRGFSLIELMIVIAIIGILIGVGIAGYR
                     AAIRAANEAAAVKTLRSIAEQQMLYFNSHQRSTFGTFEEMRKENLLDSRFDGATPVVD
                     GYVYTMKIIPKSTSAQPGYTINADPQVATGTGATGRNHYYVDSDTNTIHVNPDQPATA
                     SDPPIGQ"
     gene            3056..3595
                     /locus_tag="VFO72_06630"
     CDS             3056..3595
                     /locus_tag="VFO72_06630"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9479000.1"
                     /translation="MYQKLARLFLLVLVSGILTAHEAMGQTNPRIVREVRHELVTLPY
                     YGVFDWLEYEVNDGTVVLRGQVVRPTTKSDAEGRVKEIDGVSRVVNEIEVLPLSSQDD
                     RLRVALYRAIYNFNSPLFRYATQSVPSIHLVVKRGHATLKGVVANRADAQLAYTRARS
                     VPGLFSVKNELIVEGEQPR"
     gene            complement(3605..4018)
                     /locus_tag="VFO72_06635"
     CDS             complement(3605..4018)
                     /locus_tag="VFO72_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HET9479001.1"
                     /translation="MPEPEINATATAELVVGPPDLASSISSEHGDSFPAVLATARMVA
                     LMETAAARVLQPFLGPGELSVGVTVDITHTAPTPPGALVVANARYTGREGKLFVFEIS
                     AHDQGGEIGRGTHKRAIVETERLVRAAAKRTADLH"
     gene            4053..>4310
                     /locus_tag="VFO72_06640"
     CDS             4053..>4310
                     /locus_tag="VFO72_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9479002.1"
                     /translation="MQLAERYDVAVIGAGVFGVWTAYQLSRAGASVLLLDAYGPGNSR
                     ASSGGESRIMRLGYGPDEIYTRSAQRSLKRWQDFFTEYNSPT"
BASE COUNT          987 a         1235 c         1252 g          836 t
ORIGIN      
        1 gtgtttgagc aaagacgccg tctggaccac gcctctttcg aatcgttgcc gcaactcctc
       61 gctttcctca ccgggcagac gcgtgtccag cacgatcgta attcgccgtt cgtcttcaga
      121 agtaaactcg cgcaccgtca accggcgtga tctcgccgtc gatttccagt cgatgtgtcg
      181 caagtcatcc tgcggttgat agtcgcgcag cgaaaacaga tcgtgtcccg caccgcggcg
      241 cacggccggc attcgcccgg catacatcgg caacaagtgc agttggtcgc ttatcacttc
      301 cggcttgggg taaacaatga tgtcgacatt gcgtgcgcgc aaacggcgcc ggcggcgaaa
      361 gaacccgaac ggaaaacgtg tggacagttc aaagccgtcg atcaaaacgt gcccgcgttt
      421 cggaaacagt tgctcgactc gctgctccgc agacgcgtga tgcggcacgt aactaaaata
      481 ggccagcaat cgtttcgcgt aacggcgccg cccgcccggc tcactgcttg gaccacgcgc
      541 ttctacaaag acggagaacg acggcaacag ccgtttcgtg ttgcgcacgg tcacgatcac
      601 cggagcgggt tcagcggcaa aaatgtgatc cggaaaacgc gccgacacgg tcagatcgcg
      661 cagcgaagtg cgcgcagcga tccagccaac aaacaaagtg gaacacagca gtgaaaagac
      721 gagaaagagc aggttgtttc cggtgttcca ggcagcaaag ccaacgacga tgatgatcac
      781 cagaaacacg ccgccgccac tggtcggttc aaaaggaaaa tccaggttcg agacttctac
      841 tcgcgcggaa cgcgccagtg gaggaacgat gaagatggta agcaacagcg cgatcacgag
      901 tgagagaatc gcggcggcgc ccgcaagcgt gatgttctct gattggcgcg ccagcacagt
      961 cacgagcgcc gctgcaagtc cagcaaggac cagcagagtg cccagaatta cacgtgtgaa
     1021 cgaggtacgt tggcgagtca cagtactaat gcagtctgaa agctgggacc gcaggcgtct
     1081 cgcctgcaaa ctctcgatgg cgctcgatgt gctacctggc aggcgagacg cctgcggtcc
     1141 cagctacaaa gtgcgctagt gtacgtcaca gaggaacact gatggtcttc aaaatctcag
     1201 tcagcactgc ttcggcttct tcgctgcgac gcaacgagga cgagaagcgc gaactgacaa
     1261 caatccggtg agcgaacacg ggcaccacca gccgcttgat gtcgtccgga atacagtacg
     1321 tgcgatcttc gcttagcgcg agtgcctgcg ccgcgcggaa caaggcaagc gtgccgcgtg
     1381 gactgacgcc gagatcgagc atttccgact cgcgcgtcgc ggcaacgatg cgcatcaggt
     1441 attgcaccag agcatcgtca acagaaactg tcgtaaccgc gcgttggagt tcgacgaccg
     1501 cgtgagcgct catcaccggt tcgagttctt ccagcggatc cgcatactca cgatcgcgca
     1561 ggatcaactg ctcgtccgcg ggcgcgggat aaccaatgcg cagccgcaac ataaaccgat
     1621 ccaactgcga ttccggcaac gggtaagtgc cgtgatgttc gatcgggttc tgcgtcgcga
     1681 gcacgataaa cggcgccggc aaatgatgcg tcgcaccttc gaccgtgacg tgacgctcgg
     1741 ccatcgcttc gagtagtgcc gattgcgttt ttggtgtggt gcggttgatc tcgtcagcca
     1801 ggacgacgtt ggcgaaaacc ggtccgggtt tccactcgaa aacgccgcta tgctggttgt
     1861 aaacggagag cccaatcacg tcggaaggca gcaaatcgga cgtaaactga attcgttgaa
     1921 agttacaatc gagtgcgcgc gccagcgcgt gagcgagcgt ggttttaccg acgccgggaa
     1981 catcctcgac caataagtgt ccaccggcga tcagcgtaac gatcgcgagg cgaatggctt
     2041 cgggctttcc cttaatgacc gtttcgacag ccctttgcag ccgttcaatc tgatcgattg
     2101 cgaaggcgct gggtacttgt ccgcccttga tggtctcgag gtttcgcatg aattgtcctg
     2161 cattctacat gctaagagca cgcgtcgcac ggaaaagttc gccgccaaaa tccgcacttc
     2221 cagcgcaatg tgacctgcgc gcgcttctgt agtagactaa gcccgacaag tgggcgaaac
     2281 gagacagaaa agcggaactt aaaactcacg gtctcgtgta attcgcccga ggaggcatta
     2341 tggaacccct gaacgacaac cagaaaaccc gacgcgacac aagaaaccag cgcggatttt
     2401 cactcattga attgatgatc gtgatcgcga tcatcggcat cctgatcgga gtcggtatcg
     2461 caggttaccg agccgccatc cgcgccgcga atgaagccgc ggcagtcaag acgcttcgct
     2521 cgattgctga acagcagatg ctctacttca actctcacca gcgaagtacg tttggcacat
     2581 tcgaagagat gcggaaagag aacctgctcg acagccgctt tgacggcgcg accccggtcg
     2641 tcgatggcta cgtctacacg atgaagatta ttcccaagtc cacttcggcg cagcccggct
     2701 acaccatcaa cgccgatccg caggtggcaa ccggcacggg cgcaaccggt agaaaccatt
     2761 attacgtcga ctctgacacc aatacgatcc acgtaaatcc tgaccagccg gcgaccgcgt
     2821 ccgacccgcc gataggccag taaccaaacg ttttgtacct tagcgtgcgc cagcgatcca
     2881 atgtggatcg ctggcgtttt ttgccggtgc agtcttcaac aaaatcggaa ggaattgccc
     2941 acagacacga caaccttttt cactagtggt aggacaggca ttcctgcctg tcagagtaag
     3001 accgcgcagg taggcaggca ggcaggaaat gcctgtccta cggaggtttt gtgctatgta
     3061 tcagaaactc gcaagactgt tccttctcgt attagtttca ggaattctca cggcccacga
     3121 ggcgatgggc cagacaaatc cgcgcatcgt gcgcgaagtc agacacgaac tcgtcacatt
     3181 gccctactac ggcgtgtttg actggctcga gtacgaagtg aacgacggga ccgtagttct
     3241 gcgcggccaa gttgtgcgac cgaccacaaa gtcggatgcc gaaggtcgtg tgaaagagat
     3301 tgacggtgtc agcagggtag taaacgagat cgaggtcctg ccactgtcgt cgcaagacga
     3361 ccgattgcga gtagcgttgt atcgtgcgat ctacaacttc aattcgccgc tgtttcgcta
     3421 cgcgacgcag tcagtgccgt cgattcatct ggttgtcaaa cgcgggcatg ccacgttgaa
     3481 aggcgtcgtt gcgaacaggg ccgacgcgca gttggcctac actcgcgcgc gtagcgtgcc
     3541 gggtctgttc agcgttaaga acgagttgat tgtggaaggt gaacagccac gctgatcagt
     3601 gccttcagtg taaatccgcg gtgcgtttgg ccgcagcgcg cacgagacgc tcagtttcca
     3661 cgatcgctcg tttgtgcgtg ccgcgcccga tttcgccgcc ctggtcgtga gcactgatct
     3721 caaagacaaa cagtttccct tcgcgaccgg tgtaacgggc gttagcgaca accagagccc
     3781 cgggcggcgt tggtgcggtg tgtgttatgt cgaccgtgac gccgactgac agctcgcctg
     3841 gtccaagaaa cggttgcagc acacgcgcgg cagcagtttc catcagtgcg accattcgtg
     3901 cagtcgcgag cacggccgga aacgaatcgc cgtgttctga cgagatcgaa ctggcgagat
     3961 ccggcggtcc aacaacgagt tcggcggtag cagtggcatt aatttccggc tcgggcatgt
     4021 tggcgctaaa gcttacctag tacaattccc tcatgcaatt ggcagaaaga tacgatgtgg
     4081 cggtgattgg cgcgggagtg tttggcgtct ggaccgctta ccaactcagc cgcgcgggcg
     4141 cgtccgtgct gttgctggac gcgtatggac cgggaaacag tcgcgcgagt tccggtggtg
     4201 agtcgcgcat catgcgattg ggttatggac cagacgagat ctacacgcgc tcggcccaac
     4261 gttcgctgaa gcgctggcag gacttcttca ccgagtacaa cagcccaacc
//
LOCUS       DASTEY010000381         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1360000, whole genome shotgun sequence.
ACCESSION   DASTEY010000381 DASTEY010000000
VERSION     DASTEY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360000"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2274
                     /locus_tag="VFO72_06645"
     CDS             <1..2274
                     /locus_tag="VFO72_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479003.1"
                     /translation="VGGAIFSTIRPNIRTQNWSNFFNSEINGPGETAIFNQVRASYGR
                     TRLLFEENRDTQSLRPSRLANSLRPNERQFLLNAPLFVNDTIPSSTVVLYDRFFNFGT
                     EDILGPVGQIAIAGFSPVGVDVFNFPQQRINNTYQLADNLTLRLSDHSFTFGVDFRRT
                     ELHSDLPRNARPLLVFNGTPGAQRLPGGGAQLVGFLKPVDLTAASAPSGILQVVQASP
                     ESRVDLRYYQYNYFAQDDWRVRRNLTLSLGLRYEYNTPVSETQRRIEQTFNDASLILV
                     PGLANFLAGRQEIYDPDRNNFAPRVALAYSTNLLGANRASVVRGGYGIFYDQAIGAVV
                     SQSRNVFPNFLTLNLAGGLANQNGVGFNITDPTLPFFPCTGPNGTRFLPITQPGTLNT
                     LNPDVPLSCLAAINSSFPGGFGFTLPERKLEMPMAHHYALTWEQVLNAKNVLAVAYVG
                     TLGRQLLRLTTPNLGPNAFLIPTLVNVFNFQPNVNGLALGPGQLPSATGITGGRPVDR
                     AGAVAIYESSATSRFDSLQVQLRGRFRLGTQYQISYTFSKATDDVSDVFDLAGASALP
                     QNSFTFAGERGPANFDVRHRLAYSFIAELPSFKNHSRAFRAFFGNILVSGTGSLRTGQ
                     PFTVNSIFDVNLDGNLTDRLDNLNGLVVTGDRQQPLVLTANDPTTLLAPIGQDGRIGR
                     NTFRAGNVIELDLSISKDFFSSGTRHLILRADIFNFINRANFGVPVRWLGAAGFGQAT
                     ETITPGRRLQLELKYSF"
     gene            2504..2647
                     /locus_tag="VFO72_06650"
     CDS             2504..2647
                     /locus_tag="VFO72_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479004.1"
                     /translation="MAPLFIRTTIAGSGDNATGIGDSNRAQLAGELRGVRAEWPLAGN
                     GRG"
     gene            2592..>3164
                     /locus_tag="VFO72_06655"
     CDS             2592..>3164
                     /locus_tag="VFO72_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HET9479005.1"
                     /translation="MNCAGFAPNGRWLATGAADNSIRLWDTESGLELRALHGHSSWIR
                     SLAVSRNGELLASGSNDRTIKLWNVTTGREPLTLTGHTAPIESLLFMPGDGFIVSGGG
                     DHTVRIWDASSGHLKQTLSSHTTAVVALAVSPDGKYLASGAADGSIRIWNAESWKELR
                     LLKKHAKRITALAFGPDSKGLISGSADGVLT"
BASE COUNT          665 a          875 c          897 g          727 t
ORIGIN      
        1 gtcggcggtg cgatcttctc aacgattcgt cccaacattc gcacgcaaaa ctggtctaac
       61 tttttcaaca gcgaaatcaa cgggccaggc gagacggcaa tcttcaatca ggtgcgcgcg
      121 tcgtacggcc gcacgcggct cctgtttgaa gagaatcgcg acacgcagtc tctgcgtccc
      181 agtcgcctgg ccaattctct gcggccgaat gagcggcagt ttcttttgaa cgcgccgcta
      241 tttgtaaacg acacgattcc aagttcgacg gtggttctgt atgacaggtt tttcaacttt
      301 ggaacggaag acattctcgg acccgtcggg caaatcgcga ttgccggctt cagccctgtc
      361 ggcgtcgacg tttttaattt tccgcagcag cgcatcaaca acacatatca attggccgac
      421 aatctaacac tgcggctgag cgatcacagt ttcacctttg gcgtcgattt ccgcagaact
      481 gaattgcata gtgacctgcc acgcaacgcg cgaccgcttt tggtttttaa cggaacgcca
      541 ggcgcacagc gcttgccggg cggtggcgct cagttggttg ggtttctgaa gccggtggat
      601 ctgaccgcgg cgtcggcgcc gagcggaatc ttgcaagtcg tgcaggccag tcccgaatcg
      661 cgcgtcgatt tgcgctacta ccaatacaac tactttgcgc aggatgattg gcgcgtgcgg
      721 cggaacttaa ccctttcgct ggggctgcgg tacgaataca acaccccggt cagtgaaacg
      781 caacggcgca tcgagcaaac gttcaacgat gcttcgctga tcctggtgcc cggcctggca
      841 aattttctcg ccggccggca agagatttac gatccggatc gcaacaactt cgccccgcgc
      901 gtcgccctcg cgtactcaac aaatcttctc ggcgcgaacc gcgcttctgt tgttcgcggc
      961 ggctacggaa tcttttatga ccaggcgatt ggtgcggttg tgagtcagtc gcgcaacgtc
     1021 tttccgaatt ttctgactct gaatctcgct ggtggcctgg caaatcaaaa tggtgtcggc
     1081 tttaacatta cggatcccac gctgccgttt ttcccgtgta ctggcccgaa cggcacacgc
     1141 tttttaccaa tcacccagcc ggggacactt aacacgctga acccggacgt gccgctttca
     1201 tgtctcgcgg caatcaattc gtcgtttcct ggcggctttg gtttcacgtt gccggagcga
     1261 aaactcgaaa tgccgatggc ccaccattat gcgcttacat gggagcaggt attaaacgcg
     1321 aagaatgtgc ttgcagtggc ttatgtcggc acgctgggaa gacaactgtt gcgccttacg
     1381 actcccaacc tgggaccgaa cgcttttcta attccgacat tggtgaacgt ctttaacttc
     1441 cagccgaacg tcaatgggct ggcgcttggt ccaggacaac tgccatcagc gacgggaatc
     1501 acaggcgggc gtcctgtcga tcgcgccggc gcagtggcga tctacgagtc atcggcgact
     1561 tcgcgtttcg attcacttca agtgcaattg cgaggccggt tccggctggg tacccaatat
     1621 cagatctctt acacgttttc gaaagcgact gacgacgtat cggatgtttt cgatttggcg
     1681 ggcgcttctg ctctgccgca aaacagtttc acattcgcgg gcgagcgagg gccggcaaat
     1741 tttgatgtgc gtcaccggtt ggcatacagc tttatagccg agcttccgtc ctttaagaat
     1801 cattcgcgcg cgtttcgcgc gttcttcggc aatattttgg tttccggtac aggaagtttg
     1861 cgcaccggcc aaccattcac tgtcaacagc atttttgatg tgaatctcga tggaaatctg
     1921 actgaccggt tggacaactt gaacggcctg gttgtcacgg gggatcgtca gcaaccgttg
     1981 gttctgactg caaacgatcc cacgaccctg ctggcgccga ttgggcaaga cggccggatt
     2041 ggacgcaaca cgtttcgcgc aggaaacgta atcgaactcg acttatcaat cagcaaggac
     2101 tttttctctt ctggcacgcg ccatctgatt ctgcgcgcgg acattttcaa cttcatcaac
     2161 cgcgcgaatt ttggcgttcc ggtgcgctgg ctgggcgcgg cgggtttcgg gcaagcgacg
     2221 gaaaccatta cacccggccg gcgactacag ttagaactga agtactcgtt ctgagggatc
     2281 gggtcagtac agggagcgat agcgacctgg tcagtaatcg gcatgcaata ttcgcgaatg
     2341 attttggact ctaatggttg gaccaggtcg ctatcgctcg ctgtactgac ccgatccaag
     2401 ttctgacagg taattatgtg atgaatcgca gcaaacgaag aataaattta tcggagccgc
     2461 ggcgactggc gtgggtcgca cttgctgtca ctgtactcgg cgtatggctc ctttgttcat
     2521 cagaaccacc atcgcaggca gcggcgacaa cgccaccgga attggtgatt caaaccgggc
     2581 acagctcgcg ggtgaactgc gcggggttcg cgccgaatgg ccgctggctg gcaacgggcg
     2641 cggctgataa ttcgatccga ttgtgggaca cggaatccgg actcgagctg cgcgctctgc
     2701 atggacactc cagctggatt cgatcactgg cggtgagtcg caacggcgaa ttgcttgcgt
     2761 cggggagcaa cgatcgcacg ataaaactct ggaacgtcac gactggacgc gagcctctta
     2821 cactgactgg tcacacggcg ccgatcgaga gtctcttgtt tatgccaggc gatgggttca
     2881 tcgtctccgg cggcggcgat catacagtca gaatttggga cgcgagttct ggtcatctca
     2941 aacagacgct ttcgagtcac actactgcgg tcgtcgcatt ggcagtgagc ccggatggaa
     3001 agtatctggc gtcaggagcc gcagacggct cgattagaat ctggaatgca gagagttgga
     3061 aagaactgcg cctcctgaag aagcacgcga agaggatcac cgcgctggcg ttcggtccgg
     3121 acagcaaggg cctaatatcc gggagcgcag atggagtgct cacg
//
LOCUS       DASTEY010000382         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1030255, whole genome shotgun sequence.
ACCESSION   DASTEY010000382 DASTEY010000000
VERSION     DASTEY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030255"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFO72_06660"
     CDS             <1..839
                     /locus_tag="VFO72_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HET9479006.1"
                     /translation="LAFNADETVITILHTNDTHSQIDPLPPNDKNAGKGGVARRATLV
                     KRIRKENPNTLLIDAGDVLQGTPYFNFYKGEVEYKAMSAIGYDAGTLGNHEFDNGVEA
                     LAAALKFANFDIVSANYDVKGTVLEGRVKPYVVKTLGGIRVGLFGLGISPVALITPEN
                     FKGVTYMDPVLAARDVVKTLREKERCALVVCMSHLGYSENPRRNSVTDAQVAGQVDGI
                     DFIASGHSHLFMERPAEQTQPCGAKTLIFQVGKSGIYVGRVDFTFRTGKLIGATGRLL
                     TL"
     gene            895..1659
                     /gene="rpiB"
                     /locus_tag="VFO72_06665"
     CDS             895..1659
                     /gene="rpiB"
                     /locus_tag="VFO72_06665"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9479007.1"
                     /translation="MENDSTRDRIRALVRDVLEKAGSAPESTPSRFVNTIPETEPQAG
                     GVTRDESAKSVITEDDVRGLDQGAVLRIGEDARLTPLAADIINERKIQIVRRNSRRGS
                     KVSKLIAVGADHGGFRMKEELKGLLAQLGHKVQDFGTDSEEPVDYPDFAHAVARAVAN
                     GSSDLGIIIDGAGVGSAMTANKVPGVRAAACYSVAVARNSREHNDANVLTLGSKTITS
                     AEMREIVTAWLATEIAEDRHRKRVAKIDAIQRQYQR"
     gene            1682..2581
                     /gene="deoC"
                     /locus_tag="VFO72_06670"
     CDS             1682..2581
                     /gene="deoC"
                     /locus_tag="VFO72_06670"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9479008.1"
                     /translation="MAYSNNGDYEKLVEQITDAIVARLNSDGVEQAAMCGCTSECFHR
                     CPERMHRIVDAGAARIGLVLGETATAREWASLIDHTLLKPEATDDDIKNLCHEAMTYR
                     FASVCVNPTWVRIAACNLRGSGVPVCTVIGFPLGATLPDVKAYEARRAILDGATELDM
                     VINVGALKSGDDCLVEHDIRSVAEVAHEYDAICKVIIETALLTDDEKVRACLAAKKAG
                     ADFVKTSTGFSKGGATVADVALMRRTVGSELGVKASGGVKGLEDARKMVEAGATRIGA
                     SVGVKIAQEAAGAKPADSRSLVY"
BASE COUNT          640 a          736 c          862 g          550 t
ORIGIN      
        1 gcctggcttt caacgctgac gagacggtca tcacgattct ccacacaaac gatactcact
       61 cgcagatcga tccgcttccg cccaacgaca aaaacgcagg gaagggaggc gtcgcgcgtc
      121 gcgccacact cgtcaaacga atcagaaaag agaacccaaa cacgctgctg atcgatgcgg
      181 gcgatgtgct tcagggcacg ccgtatttca acttctacaa aggcgaagtt gagtacaaag
      241 cgatgtcggc cattggttac gacgcgggca cactcggcaa tcacgagttt gacaacggcg
      301 tcgaagctct cgcggcggcg ttgaagtttg cgaatttcga catcgtgtcg gcgaactacg
      361 acgtgaaagg caccgtgctc gaaggacgcg ttaagcccta tgtcgtaaag actctcggcg
      421 gaatacgggt tgggctattt ggtttgggca ttagccctgt cgccctgatc acgccggaga
      481 atttcaaagg tgtaacgtac atggatccgg tgctggcggc tcgtgacgtg gtgaaaacgt
      541 tgcgcgagaa agagcgttgc gcgctcgtgg tttgcatgtc gcatctcggt tactctgaga
      601 atccgcgacg caacagtgtt actgacgcgc aagtggccgg gcaggtggat ggcatcgact
      661 tcatcgccag cggtcacagc cacctgttca tggaacgtcc cgccgagcag acgcaacctt
      721 gcggcgcgaa gacgttgatc ttccaggtcg gcaagagcgg gatctacgtc ggccgcgttg
      781 atttcacgtt cagaaccggg aaactgattg gcgctaccgg acgtttgttg actctgtagg
      841 acagacattc ttgtctgtca catcacgaca ggcaggaatg cctgtcctac gaacatggaa
      901 aacgactcaa ctcgcgatcg aatccgtgcg ctggtgcgcg acgtgttgga gaaagccgga
      961 tctgcgccgg aaagcacgcc gagtcgcttc gtaaacacga ttccagagac tgaaccgcag
     1021 gcgggtggtg taacacgtga tgagtcggcg aagagcgtca tcactgagga cgacgtgcgc
     1081 ggattggacc agggcgcggt attgagaatc ggtgaagacg cgcgtttgac gccgctcgcc
     1141 gccgatatca tcaacgagcg caagattcag atcgtgcgac gcaactcgcg acgtggatcg
     1201 aaagtttcaa aactgatcgc tgtcggggcg gatcacggcg gcttcaggat gaaagaggaa
     1261 ttgaagggcc tgcttgcgca actcggacac aaagtgcagg acttcggcac cgactcggaa
     1321 gagccggttg attatccgga tttcgcgcac gccgtcgcac gcgctgtcgc taacggcagt
     1381 tctgacctcg ggataattat cgatggagct ggtgttggca gtgcgatgac ggcgaataaa
     1441 gttccgggag ttcgggctgc ggcgtgttac tcggtggcag tggcgcgcaa ctctcgcgaa
     1501 cacaacgatg cgaacgtcct cacgttggga agcaaaacaa tcacgagcgc ggagatgcgc
     1561 gagatcgtca ccgcctggct cgccaccgag atcgccgaag accgccaccg caagcgcgtc
     1621 gccaagatcg acgccatcca gcgacagtat caacgctagt tggtaaaatg ttgcccgtcc
     1681 catggcctac tccaataacg gcgattacga gaaattagtc gagcagatta ccgacgcgat
     1741 tgtggcgcgg ctgaatagtg acggcgtcga gcaggccgcg atgtgcggtt gcacgtccga
     1801 gtgctttcat cgctgtcccg agcggatgca tcgcattgtc gacgccgggg ccgcgcgtat
     1861 tggattagtt ttaggtgaaa ctgcaacggc gcgggaatgg gccagcctca tcgatcacac
     1921 gctactgaag cctgaagcta cagacgacga catcaagaac ctctgccacg aagcgatgac
     1981 ctaccggttc gcgtcggttt gtgtgaaccc aacgtgggtt cgcattgctg catgtaatct
     2041 gcgcggcagt ggcgtgcctg tttgcactgt gatcgggttt ccactcggag caacgctgcc
     2101 ggacgtcaaa gcgtacgagg cgcgccgcgc gattctcgac ggagcaacgg aactcgacat
     2161 ggtgatcaat gtcggcgcgt tgaagagtgg cgacgattgt ctcgtagaac acgacatacg
     2221 ctctgttgcc gaagtcgcgc acgagtacga cgcgatctgc aaagtgatca tcgaaacggc
     2281 actactgact gatgacgaaa aggtgcgtgc gtgtctggca gccaaaaaag ctggggcgga
     2341 ttttgtcaag acatcgacgg ggttttcgaa aggcggtgcg acggttgctg acgtggcgtt
     2401 gatgcggcgc acggttggat ctgaactcgg cgttaaagct tcaggtggag taaaaggtct
     2461 cgaagacgcg cggaaaatgg ttgaagccgg agcgacgcga atcggcgcga gtgttggcgt
     2521 aaagatcgct caggaggccg ccggtgcaaa gcccgcagat tccagatcat tagtgtacta
     2581 gtagtagcga aagctctttc ctgggaccgc aggcgtctcg cctgcatcgc tcggttttcg
     2641 cgcttcgcgc tcagtgcagg cgagacgcct gcggtcccag gaaagagcat ccgcgtgatg
     2701 tgaggatcgt tagcgtacta acaatcggca atcggaaatt ggcaatcgga aatcaacaat
     2761 cgattcatgc ctgcggatcc caaacttt
//
LOCUS       DASTEY010000383         5320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_436946, whole genome shotgun sequence.
ACCESSION   DASTEY010000383 DASTEY010000000
VERSION     DASTEY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436946"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFO72_06675"
     CDS             complement(<1..328)
                     /locus_tag="VFO72_06675"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9479009.1"
                     /translation="MDFVPLTCDQRRAFAEDGFLVVPNALDRDTVERLLSEADRLAEP
                     FLRKPVIAGKPEYNHLDLRRGLLKQKALFALVSNSTTVPLVVQLLGPNIHLHSTTVIY
                     KRPETAA"
     gene            complement(328..1086)
                     /locus_tag="VFO72_06680"
     CDS             complement(328..1086)
                     /locus_tag="VFO72_06680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9479010.1"
                     /translation="MAFLSTKTGQFTYFAQQLGESNWRRKNVLDFGGNIGNILRDPNS
                     TIDHERYWCIDVVRESIEKGKAEFPDAHWVFYDRYCFFFNPFGVRDLALPSLDHRFDY
                     IVAYSVFTNTPRTDMLQLVEQLKNQLATGGALAFTFIDPHYHSWPDRYAGNNLVWRLE
                     REILLEGEKGNTLDIDVAALSEGAKDASWFVLVNGEDLYLETENVPACEPERQRTYHT
                     FYTVDHMRSLFPEATILPPANDEMQHCCVIKKAF"
     gene            complement(1121..1477)
                     /locus_tag="VFO72_06685"
     CDS             complement(1121..1477)
                     /locus_tag="VFO72_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479011.1"
                     /translation="MTTGNAASLLGHWLNTNRDSRGIAECVIAEDKEEITVSITGASD
                     KETWPIERATVLANVEEEAGQRSVALLATFNFGDRIAETHIRVNKGVLVIVLYVTFHD
                     DSGRSNYLNREFFYRS"
     gene            complement(1477..1857)
                     /locus_tag="VFO72_06690"
     CDS             complement(1477..1857)
                     /locus_tag="VFO72_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479012.1"
                     /translation="MKMELGALLGTWINSNPDTHSVALVKATESGGRLKLQVSAVGAD
                     GFVDWGTADAEVFAAAPDSHNGAGFTCRYKFDYGETRLQGMIMKGLLVLAQFHLFNDE
                     SKRENYFLREYFAATHDRFSKANS"
     gene            complement(1854..2738)
                     /locus_tag="VFO72_06695"
     CDS             complement(1854..2738)
                     /locus_tag="VFO72_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET9479013.1"
                     /translation="MLAPLPNSARLKVEPALARDLREAAERLPHYDNKEFYSTELQTF
                     VYQALQEVNGFDSLTDEIRQRLSARPYCVLVQGLSFDEGNKVFVGINRAFGELVARPY
                     EAPRAQLVHYIQPATDIPSARGGHESERLHTDTADWEPPVELISMVCVRADPDGGGRS
                     RVLDIDSIRDEVKHQLGAAVLQRLETERVPWQLASYCGGGVLWRSILTKRSICWRRYT
                     IDMALDSTGATLSPEMLSALEAFENVLSRTPRTIEFLMREGELLFSDNLRTIHARTPI
                     AAGANSNRLMIRSWVRKE"
     gene            complement(3158..3946)
                     /locus_tag="VFO72_06700"
     CDS             complement(3158..3946)
                     /locus_tag="VFO72_06700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9479014.1"
                     /translation="MTIEEVHLWRGTPAERALRSRLVKSSQFAYFNEQLDYPDWSGKV
                     VLDFGGNKGHLLLDPLCAIRQQDYYCLDVISEAIEEGRRTFPGAHWTHYDRYNRSFNP
                     EGKRDLPIPELGLQFAFILSYSVFTHTTLEEMKDLVVQLERFLAPGGTLAFTFIDPHW
                     RSNLEWRLKRSQAPDIDTLVAKSSKADWCSLINGTQLYVNSSGPWIVSPETCATYNVY
                     YSEAFLRRLFPRAEIRPPVNNEMQHCAILRKDGLCTKDRITAST"
     gene            complement(4017..4925)
                     /locus_tag="VFO72_06705"
     CDS             complement(4017..4925)
                     /locus_tag="VFO72_06705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9479015.1"
                     /translation="MTNPVLSAIIPTWNRAHLIGEAIESALSQRAGEVEVIVVDDGST
                     DDTVALITNNFGSRVRVLELETRRGPGAARNAGVLLASGEFVAFLDSDDLWLPGKLDA
                     ELRVFAASPEAIAVISDSENFFEGESDPASRFAQNGLLATTDGRVLCVDDCDWLWTNS
                     TISAQTSGIIVRRKVLAQLGRTLFGEDVLCCEDWEFQLRLYHHGQVMVLPEIYSTVRR
                     FNDGSRIGRGIPGRPLTTDHEITLLRARLTIIERSRPWLRGVRADLVAELERFRAETE
                     ARLAEVSLTNSEFSRKGAKAQRYAAV"
     gene            complement(4922..>5320)
                     /locus_tag="VFO72_06710"
     CDS             complement(4922..>5320)
                     /locus_tag="VFO72_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9479016.1"
                     /translation="ENGCLRVVPRSHRLGLIPHADRPNPHNLTTQGATAQVQIDEPQD
                     IVMQAGEMSLHHPLVLHASNPNRSGSSRIGFSATYSTPALTASRTAVAWVRGDGPRDR
                     FRIVEKPAARSIEAAAAAYLEADHQILFAP"
BASE COUNT         1211 a         1527 c         1445 g         1137 t
ORIGIN      
        1 ccgcggcagt ttcgggacgc ttgtagataa ctgttgtcga gtgcaggtgg atgttcggtc
       61 cgagcagttg gaccacgagc ggcacagtcg tcgagttcga caccagcgca aacaacgctt
      121 tctgcttcaa caagccgcga cgcaaatcca ggtggttgta ctccggcttg cccgctatca
      181 caggcttccg cagaaatggt tccgccagac gatcggcttc gctcaaaaga cgctcgaccg
      241 tatctcgatc gagtgcgttc ggcacgacga gaaaaccgtc ttcagcaaac gcgcgacgct
      301 gatcgcaagt gagtggcaca aagtccatca aaaagccttc ttaatcacgc agcaatgctg
      361 catctcatca ttcgcaggcg gcaagattgt cgcttcggga aacaacgatc gcatgtggtc
      421 gacagtgtag aacgtgtgat acgtccgctg ccgttcgggt tcgcacgctg gaacgttttc
      481 tgtttccaaa tacagatcct cgccgttaac cagcacaaac caacttgcgt cctttgcacc
      541 ctcgctgagg gcggcgacgt cgatatcgag cgtgttgcct ttctctccct ccagcaagat
      601 ctcgcgttcg agccgccaga ccaggttgtt tcccgcataa cgatcgggcc aggagtggta
      661 atgcggatcg ataaacgtaa acgcgagcgc gccgcccgtc gctaattgat tcttcaactg
      721 ctcgaccaac tgaagcatgt cggtgcgcgg cgtgttagtg aacaccgagt aggcgacgat
      781 gtagtcgaat cgatgatcga gcgacggcag cgcgaggtcg cggactccaa acggattaaa
      841 aaagaaacag tagcgatcgt aaaagaccca gtgcgcatcg ggaaactccg cctttccttt
      901 ttcgatcgac tcgcgcacga cgtcgatgca ccagtaacgt tcgtgatcga tcgtcgagtt
      961 gggatcgcgc aggatgttgc cgatgtttcc gccgaaatcg aggacgttct tgcggcgcca
     1021 gttggattcc ccgagttgct gtgcgaaata agtgaattgc cccgttttcg ttgaaagaaa
     1081 ggccacggac tacacggttt tcccagaacg atgaaagttt ctaagaacga taaaagaatt
     1141 cgcggttgag atagtttgat cggccgctgt cgtcgtgaaa cgtgacgtac agcacgatga
     1201 cgagcacgcc tttgttcact cgtatgtgag tctcagcgat ccggtcaccg aaattgaacg
     1261 tcgctaacag agcaaccgat cgttgcccgg cttcctcttc gacgttggcg agtacggttg
     1321 cacgctcgat gggccatgtt tctttgtcgc tcgctccagt gatgctgacg gtaatctcct
     1381 ctttgtcctc tgcgatgaca cactcggcaa taccgcgact gtcgcgattt gtgttcaacc
     1441 agtggccgag caggctggcg gcgtttccag ttgtcatcat gagttagcct ttgagaagcg
     1501 atcgtgagtc gcggcgaaat attcgcgcaa aaaatagttc tcgcgcttac tctcgtcatt
     1561 gaacagatga aattgggcca gcaccagcag gcctttcatg atcattccct gtaaacgtgt
     1621 ctcgccgtaa tcgaacttgt agcggcaggt gaagcccgcg ccgttgtgtg aatcaggtgc
     1681 cgcggcaaat acttcagcat cggcagtacc ccagtcgacg aacccgtcgg caccgaccgc
     1741 tgaaacttgc agtttgagtc gcccgcccga ttcggtggct ttgacgaggg ccacgctatg
     1801 agtatcaggg tttgaattaa tccacgtgcc gagtagcgca ccgagttcca tcttcattcc
     1861 tttcttaccc agcttcgaat catcaaacga ttggagtttg cgccggcggc aatcggcgtg
     1921 cgcgcgtgaa tcgtgcgaag gttgtcggaa aagagcaact cgccttcgcg catcaaaaac
     1981 tcgatcgtgc gcggtgtgcg cgaaaggacg ttctcgaaag cctcgagcgc gcttagcatc
     2041 tcgggcgaga gagtcgcgcc ggtcgaatcg agcgccatgt cgatcgtgta gcggcgccag
     2101 cagatactcc gcttggtcaa tatggatcgc cacagcacgc cgccgccaca ataacttgcc
     2161 agttgccacg gcacacgttc agtttccagg cgctgcaaca ccgcagcgcc aagctgatgc
     2221 tttacttcgt cacgtatcga atcgatgtcg agcacgcgtg aacggccgcc accgtcagga
     2281 tcggcacgaa cgcaaaccat cgagatcagt tcgactggcg gttcccaatc ggcggtgtcg
     2341 gtgtgcaggc gttcactttc gtgaccgcca cgcgccgaag gaatgtcagt cgcgggttga
     2401 atataatgga ccaactgcgc gcgcggcgct tcataaggcc gcgccaccag ctcaccaaac
     2461 gcgcgattga tgccgacaaa aaccttgttg ccttcatcga atgacaaccc ctggaccaac
     2521 acgcagtacg gccgggcgga cagcctctgc cggatctcat cagtcagcga atcgaatccg
     2581 tttacctctt gtaacgcctg atacacgaac gtctgcaact cagtagagta gaactcctta
     2641 ttgtcgtagt gcggcagacg ttcagcggcc tcgcgcagat cgcgggccag cgctggctca
     2701 accttgagtc ttgcactgtt cgggagcggc gcgagcattt ctatgtatcc acttcctttc
     2761 tgctttgcac aaaaaaccac gccgaaggcg ttcgcaaggt caagcccagg gtctataccc
     2821 tgggaacaac gaatttcacc ccgcccgcgg aactctgtaa gagttcgcca actccttcgg
     2881 agttgtgtaa ttgagaggac gatactcccc cagggttgct ttgcctcaaa agtcctctct
     2941 cgttctcacc gggctttagc ccggtgcagg agcagtgaag acggaggaaa ccgtttcaac
     3001 ggttttccgt tgattcacca gcgaaaccgt tgaaacggtt tctcaaaacc gcgtggccgt
     3061 aacaccgggc tgaagcccgg tgagaatgaa agaagacttt tgagcaaagc ccccagggta
     3121 taaaccctgg gttgcaattt gcgaactcct tcggagttca agtcgaagca gttatccgat
     3181 cttttgtgca aagcccatcc tttcgcagga tcgcgcagtg ctgcatttca ttgttgactg
     3241 gcgggcgaat ctcagcccgc ggaaacagtc gtcgcagaaa cgcttcgctg tagtagacgt
     3301 tgtaggtagc gcacgtttcg gggcttacga tccacggtcc gctgctgttc acgtagagtt
     3361 gggtgccgtt tatgagtgaa caccaatccg ctttgcttga cttggcgacc agagtatcta
     3421 tgtccggcgc ctggcttcgc ttcaaacgcc actcgagatt cgaacgccag tgcggatcga
     3481 tgaaggtaaa agccagcgtt ccgccgggcg ccagaaatcg ttccagttga acgacgagat
     3541 ctttcatttc ctcgagagtc gtatgtgtga ataccgagta ggaaaggata aaagcaaact
     3601 gcaaccccag ctcgggaatg ggtaagtcac gcttgccttc gggattgaac gagcgattgt
     3661 accgatcgta gtgcgtccaa tgcgcgccgg gaaaagtgcg gcgcccttct tcaatcgctt
     3721 cgctgattac gtcgagacag taataatcct gttgacgaat cgcgcacaac ggatcgagta
     3781 gcagatgacc cttgttgccg ccaaaatcga gcaccacctt accactccag tcaggatagt
     3841 cgagttgttc gttgaagtaa gcaaactgcg acgacttcac caaccgcgaa cgtagcgcgc
     3901 gttccgccgg tgttccccgc cacagatgta cttcttcgat cgtcatgagc tacaaacgtc
     3961 tcacgcaaag gcgccagggc gcaaagatgc agagaagcga gacgcaaaga aacctgtcag
     4021 acagctgcat acctttgcgc cttcgcgcct ttgcgcgaga actcactgtt cgtcaatgat
     4081 acttcagcca aacgcgcctc cgtctccgca cgaaaacgct ccagctccgc gacgagatcc
     4141 gcacgcactc cgcgtagcca cggccgcgat cgttcgatga tggtgagacg cgcgcgcaat
     4201 aatgtgatct cgtggtcggt tgttagaggt cgtcccggaa taccacgccc aatgcgtgaa
     4261 ccatcattga agcgccggac ggttgagtag atttccggga gcaccatcac ctgcccgtga
     4321 tgatagagac gcaattgaaa ctcccaatcc tcacagcaca atacatcttc gccaaacaga
     4381 gtacgcccga gttgggccag caccttacgc cgcacaatga tgcccgaggt ctgcgcgctg
     4441 atcgtgctgt tggtccatag ccaatcgcaa tcgtcgacgc agagcacgcg gccatcggtc
     4501 gtggccagca gaccgttttg cgcaaaacgg ctggccggat cgctttcgcc ctcgaaaaag
     4561 ttctcgctat cggagatcac ggcaatggct tcgggagacg ctgcgaacac gcgcagttcg
     4621 gcatccagtt ttccaggtaa ccagagatcg tcagaatcca gaaaggcaac gaactcacct
     4681 gaagccaaaa gcactcctgc atttcgcgct gctccgggac cgcggcgtgt ttcaagctcc
     4741 agcacgcgaa cccgcgaacc aaagttattt gttatcaaag ctaccgtatc gtcggtcgaa
     4801 ccgtcgtcaa ctacgatgac ctcaacctcg ccggcacgtt gactgagcgc actctcaatc
     4861 gcctcgccga taaggtgagc acggttccag gtcgggatga tggccgatag aactggattg
     4921 gtcacggggc aaacagaatc tgatgatccg cttcaaggta cgcggcggca gcggcctcga
     4981 tcgaacgtgc cgccggtttt tctacgatcc ggaaacggtc tcttggacca tcgccgcgca
     5041 cccacgccac ggccgttcgg cttgcagtca acgccggagt tgaataggtc gcagagaaac
     5101 cgatacgcga cgagccggaa cgatttggat ttgaagcatg cagcacaagc ggatgatgca
     5161 gactcatctc gcccgcttgc atcacgatgt cctgtggctc gtcgatctgc acctgcgcgg
     5221 tcgctccctg tgtggtcagg ttgtgcgggt tcggacgatc cgcatgcgga atcaaaccca
     5281 aacgatgcga acgcggcacg acgcgtaaac aaccattctc
//
LOCUS       DASTEY010000384         8108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_272140, whole genome shotgun sequence.
ACCESSION   DASTEY010000384 DASTEY010000000
VERSION     DASTEY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272140"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFO72_06715"
     CDS             <1..1342
                     /locus_tag="VFO72_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479017.1"
                     /translation="LLLQGTNFVNFMGRYVYVAADHSLEVVVATEHTEPQAVIGSTLQ
                     KIAYPSNYDRFVRGGRQLKTFYEHVGNPRVLQVQLRGEYAYVAAGEGGLRVYDVAQID
                     HKGFSERIVTAPVSRFGQKFWVKTKYATAVAAPATLAVDPARTRRPENEEQPIHPLYA
                     YLYVADKYEGLILVNAATLLDGDPLNNYLKRARPAFNPAGALNGANNIAIAGTYAYIT
                     TDSALVVVDINEPLNPRIVRQIPVKKPRSVAIQFRYAFVVDQEGLKTVDVTVPQQARL
                     VDGALVPLVDAHDVYVARTYAYVANGKEGIAIIDVEQPERPLLDQTYNAGGQLNDVHQ
                     VKIAMTNASLFAYVADGKNGLRILQLTSPETMPEYAGFSPRPRPELIATFKTKGDALA
                     VSKGLDRDRAADESGNQVSVFGRRGARPFTYDEILRLLRTNDGKGEWFRVKDHR"
     gene            complement(1350..4091)
                     /gene="mutS"
                     /locus_tag="VFO72_06720"
     CDS             complement(1350..4091)
                     /gene="mutS"
                     /locus_tag="VFO72_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9479018.1"
                     /translation="MNQSNTTPMLRQYQELKQQHPGTLLFFRLGDFYELFFDDALTGS
                     RELQITLTARHKETGNPVPMCGVPHHSAANYIARLVRKGYRVAICEQTEEAGKTKKLV
                     RREVVRVVTPGTPIDPQLLEAREPVFLAAVCTAGESVGAAFLDISTGEFRVTQETGRD
                     SWTRIRAALESYAPRELLFPASLAALIKSGLSGKTQTAPLPLRSDGLEQHGFSENEIG
                     FSATLTPIDDWKWQRDDCHALLLEHFKVQFLDGYGLVRKDEAIRAAGACLRYARETQR
                     ATATHVTDLVYFEPQDHLVLDNVTVRNLELVESLAGGSGRSLLQVIDETVTGMGARLL
                     RSWLLRPCVKRAEVEARLAAVQNLFASQMRRDKLRALLKEVSDLERLVGRVSLGSATP
                     RDLVAMLKSLNQVPEIREVLRAFESSLLEVLAENTDELPDIRALIANAITDDPPVKLP
                     DGNTIRAGFSAELDELRSTSRNAKQIIAALEASERTRSGIGNLRIRFNGVFGYFIEVS
                     KANAARVPPEYERRQTLANAERFTTPELREWERKVLGADERIIQLETELFAGVCRQIA
                     NETKRIQMTARALAALDALAAMAETSARRRYARPLLHDGDELEIVQGRHPVIEAFIDE
                     PFVPNSVYLNNSTDRLLIITGPNMGGKSTILRQTAIICILAQTGCFVPAERARLPLLD
                     RIWTRVGASDDLTRGRSTFMVEMTETAAILHSATPRSLVLLDEIGRGTATFDGLSIAW
                     AVAEYLHDSADHAAKTLFATHYHELTELAERLPGAQNYQITATEREGEVVFLHRLERG
                     RASKSYGIEVARLAGLPPAVLATAREVLARLERYELDVFAEQEPQIEHELAVKAVAAN
                     GAIDKAVSRAGRRKVAAQASLFDLANQKVIEELRSSPDSLTGDEAKDLICKLKDHLV"
     gene            4150..4713
                     /locus_tag="VFO72_06725"
     CDS             4150..4713
                     /locus_tag="VFO72_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HET9479019.1"
                     /translation="MNNLLNRSLQEHLETIQQLLESSLPQIEESGRLICDALSSGKKI
                     LLCGNGGSAADAQHIAAELVGYYENQRRSWPAVALTTDTSALTAVSNDLGFEQVFARQ
                     VAGLAQAGDVLIAISTSGKSKNVLRAVEQAKELGCKTIALTGASAETLSSLCDVVVTV
                     PATRTSRIQEAHITIGHLWCEMVDLFS"
     gene            5137..5424
                     /locus_tag="VFO72_06730"
     CDS             5137..5424
                     /locus_tag="VFO72_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479020.1"
                     /translation="MTIKPPTQGKKKKRKKKTALVVCRDRKQFWTTQTQFWQWVREGI
                     VTKVGDQPLTGMFRREHEELMVLLSNTILNLAHPNHMREALLSRRIGMAGK"
     gene            5454..6374
                     /locus_tag="VFO72_06735"
     CDS             5454..6374
                     /locus_tag="VFO72_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9479021.1"
                     /translation="MRKVLVTGILLMMAVSVHAQQDDRYRIGPGDVLDIRIYNRPQLS
                     REAVRVEGSGMIRMPLIESEIQAVCLTEGELAKEIATRYAKYYRNLQVDVFIREFHSR
                     QVAIIGAVNDQSRFELQRRVSLLELLTYAKGPSTKAGQTVNIVHSTTTSPCKQATDNE
                     DTAALTSYKLSDVLEGKPESNPYLEAGDIVTIPEADQVYVVGNVFMPLTIPLREPITL
                     TRAIAMAGGLKQDTRKDKIRVLRQEPGSSVRKEITVDLYAIEKRRSEDLALAPNDIID
                     VPTSAGKSFLRSLIQGVVPGVGQLPVRVVP"
     gene            6397..>8108
                     /locus_tag="VFO72_06740"
     CDS             6397..>8108
                     /locus_tag="VFO72_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HET9479022.1"
                     /translation="MKPPTDLVKQEPAAALERVERPQSYNYGVDPNAQHEVHLIDYWR
                     AIRKRIWMVLGLVALITMIAIVHVARKPDYFESQARVQVKSEDNSTLTGKTPYFYSAS
                     EDSIYFNTQLQILTSQGLMRRVVRTLDLEHNPDFFKGSNAQKRSTWRTILEMAGLGRD
                     ANTSASPDQLPLTSSVAPATSREDLTEAKRLAPYVAAILGGLRVDPVKEARGYYKETS
                     LIDIRYSHTDPHVAAKVVNAIADTYVYANLEKKIETNSSTGTFLQKRIAELQQQIRTD
                     EERLVNYARNNQIISLDPTQNTVVERLAGLNRQLLEAENERKTAEAAFTAASAPGAAE
                     ALAENDAKQVSDLETKLNDLRQKRAQLLVEATEEAPEVKEVDQQIAELNKQLKDLRSR
                     KSSALLTTLETRYKQALGREQALRKSFDEQRAETLTQNEAAINHRIIQQEIETNKSLL
                     NSLLQGAKENDVVIAGKPNNISVVDYGLAPDSPVGPNRTRTIITAFFLAIGLGVGFAL
                     FLEYLDDTVHTTEEVERGLQLPALAVIPSVGARVSRNRLIDTSKALLKRNGNGHVDRS
                     EELL"
BASE COUNT         1828 a         2213 c         2386 g         1681 t
ORIGIN      
        1 gttgctgttg cagggcacta acttcgtcaa cttcatgggg cgatatgttt acgtcgctgc
       61 cgatcactcg ctcgaggttg tcgttgccac ggagcacacc gagccgcagg cagtcattgg
      121 cagcacgttg cagaagattg cgtatccgtc gaactacgat cgttttgtgc gcggcgggcg
      181 gcagttgaag acgttctacg agcacgtggg gaacccgcgt gtgttgcagg tgcagttgcg
      241 tggtgagtat gcgtatgtgg cggcgggtga aggcgggctg cgtgtttacg acgtggcgca
      301 gatcgatcac aaagggtttt cggaacggat cgtgacggcg cccgtttcgc gtttcgggca
      361 gaagttttgg gtgaagacga agtatgcgac agcggtggca gcgccggcga cactggccgt
      421 agatccggcg cggacgcggc ggccggagaa tgaagagcaa ccgatacacc cgctttacgc
      481 gtacttgtac gtcgccgata agtatgaagg gttgattttg gtaaatgcgg cgacgttgtt
      541 ggatggcgat ccgttgaata actatttgaa gcgtgcgcgt cccgcgttca atcccgccgg
      601 tgcgcttaac ggtgcaaata acatcgcgat tgccgggact tacgcgtaca tcactacaga
      661 cagtgcgctg gttgttgttg atatcaacga gccgctaaat ccccgcatcg tccgtcaaat
      721 tcccgtcaaa aaacctcgct cagtggcgat tcagtttaga tatgcgtttg ttgttgatca
      781 ggaaggtctg aaaactgtgg acgtgaccgt gccgcagcag gcgcgtctcg ttgacggcgc
      841 acttgttccg ctcgtggatg cgcacgacgt ttacgttgcg cgcacttatg cgtacgtcgc
      901 aaatggaaag gaaggaatcg ccatcatcga cgtcgaacaa ccagagcgac cgctgctgga
      961 ccagacgtac aacgcgggcg gacagctcaa cgacgtacat caggtgaaga tcgcgatgac
     1021 gaacgcgagt ctgtttgctt acgtggctga cgggaagaac gggttgcgga ttttgcagct
     1081 tacttcgccg gagacgatgc cggagtacgc gggattcagt ccacggcctc ggccggaact
     1141 gatcgcgacg ttcaaaacca agggggatgc gttagcagtt tcgaagggct tggatcggga
     1201 tcgggccgct gacgagagtg gaaatcaggt ctccgtgttt ggacgtcgcg gcgcgcggcc
     1261 gtttacgtat gacgagatcc tgcgcttact cagaactaac gatgggaagg gtgagtggtt
     1321 tagagttaag gaccacagat gacacggact tacaccaagt ggtccttcag tttgcaaatg
     1381 agatctttgg cttcgtcgcc cgtgagtgag tcgggactgg aacggagttc ctcgataacc
     1441 ttctgattgg ccaagtcaaa cagggatgcc tgcgctgcga cttttcgccg gccggcacga
     1501 ctcactgcct tatctatagc gccattcgcg gcgacagcct ttacagctaa ttcgtgttca
     1561 atctgtggct cttgttcggc aaaaacatcc agctcgtaac gctccagccg cgccaacact
     1621 tcgcgcgcag tggcgagcac ggccggcggc aggcccgcca gacgcgcgac ctcaatgccg
     1681 taagacttcg acgcacgccc acgctcgagc cggtgtagaa atacaacttc gccttcacgc
     1741 tcggtggcgg tgatctgata gttctgcgca cctggaagac gctccgcaag ttcagtgagt
     1801 tcgtgataat gcgtggcaaa gagtgtcttc gccgcgtgat ccgccgagtc gtgcaggtat
     1861 tcggcaacgg cccaggcaat cgacaaaccg tcgaacgtcg cagttccgcg tccgatctcg
     1921 tcgagcaaca caagcgaacg cggagtcgca ctgtgcagga tcgcggcggt ctccgtcatc
     1981 tcaaccatga acgtcgaacg gccacgcgtg agatcgtcgg aggcgcccac acgcgtccag
     2041 atgcgatcca gcagcggtaa acgtgcgcgt tcggcaggaa cgaaacatcc ggtctgcgcg
     2101 agtatgcaga tgattgcagt ctggcgcagg attgtgcttt tgccgcccat gtttggcccg
     2161 gtgatgatca gcaggcgatc ggtcgagttg ttgagataga cgctgtttgg tacgaacggt
     2221 tcgtcaatga acgcctcgat tacgggatgg cggccctgga cgatctccaa ctcatcgccg
     2281 tcgtgaagca atggccgcgc gtagcgacgg cgtgctgaag tttcggccat cgcggcgagc
     2341 gcatcgaggg ccgcaagtgc gcgcgccgtc atctgaatgc gcttggtttc gttggcgatc
     2401 tggcggcaga caccggcgaa cagttccgtt tcgagttgaa tgattctttc gtcagcgccg
     2461 aggacttttc tttcccactc gcgtaattca ggcgtggtga agcgttcggc gtttgcaagt
     2521 gtttggcgtc gctcgtactc gggaggcact ctcgccgcgt ttgctttcga cacctcgatg
     2581 aaatagccaa agacgccgtt gaaacgaatg cgcaggttgc cgatgccgct gcgtgtgcgc
     2641 tccgaggctt cgagcgcggc aatgatctgt ttcgcgttgc gactggtgga acgcaactcg
     2701 tcgagttccg cggagaagcc ggcgcggatt gtgttgccgt cgggtagttt caccggcgga
     2761 tcgtcagtga tcgcgttggc gatcaacgca cggatgtcgg gcagttcatc agtgttctcg
     2821 gcgagcactt cgagcaacga cgactcgaac gcgcgcaaca cttcgcggat ctcgggcacc
     2881 tggttcagcg acttcagcat cgcgaccaga tcgcgcggag ttgccgatcc aagactgact
     2941 cgtccaacca gacgttccag atccgagacc tctttcaaca gcgcgcgcag cttgtcgcga
     3001 cgcatctgag acgcaaatag attctgaacg gcggccagtc gcgcttcgac ttccgctcgc
     3061 ttcacacacg gtcgcagcag ccacgatcgc agcaaacgcg cgcccatgcc ggttacggtc
     3121 tcatcaatca cttgcaggag cgaacggcca ctgccacccg cgagcgattc gacgagttcg
     3181 agatttcgca ccgtcacatt gtcgagcacg agatggtctt gcggttcgaa gtagaccaga
     3241 tcggttacgt gtgtggctgt cgcgcgctga gtttcgcgtg cgtaacgcag acaggcgccc
     3301 gctgcgcgga tcgcttcgtc tttacgcacc agtccatagc cgtcgagaaa ttggaccttg
     3361 aagtgctcga gcaagagcgc gtggcaatcg tcgcgttgcc atttccaatc gtcgattggc
     3421 gtcagtgtcg cagaaaatcc gatttcattt tccgaaaatc cgtgttgttc caatccgtcg
     3481 cttcggagcg gtaacggggc tgtttgcgtt ttccccgaca aaccactctt gattaacgca
     3541 gccagcgaag caggaaacag caactcacgc ggtgcgtacg actcgagcgc ggcccgaata
     3601 cgcgtccagg agtcgcgtcc ggtttcctga gtcacacgaa attcgcctgt cgaaatgtcg
     3661 agaaacgcgg cgcccacgct ctcgccggcg gtgcagacag cggcgagaaa aaccggttcg
     3721 cgtgcttcaa gcaattgcgg atcgatgggc gtgcccggcg ttacgactcg gaccacttcg
     3781 cggcgcacga gtttcttagt tttgccggct tcttcagtct gttcgcaaat cgcgacgcga
     3841 taacccttgc ggacgagacg tgcaatgtag ttggccgcgg agtgatgtgg cacgccgcac
     3901 attggcacgg gatttccggt ttccttgtga cgcgccgtga gggtgatttg cagttcacgc
     3961 gagcccgtga gtgcgtcgtc aaagaagagc tcgtaaaagt cgccgagacg gaagaacagc
     4021 agagttccgg ggtgttgctg cttgagttcc tggtactgcc gcagcatcgg cgttgtattt
     4081 gattggttca tagtttgtga gcgccacaga ttaacaccga aggggcggtt tgtggtaatt
     4141 tcagcgcgcg tgaacaatct cctaaacagg tctcttcagg aacatctcga aacaatccag
     4201 cagttacttg agtcttcgct gccgcagatc gaagagtcgg gccggctgat ctgcgacgca
     4261 ttgtcaagcg gaaagaaaat tcttctttgt ggcaacggtg gcagcgcggc tgatgcgcag
     4321 cacatcgccg ccgaactcgt cggttattac gaaaaccaga gacgttcatg gccggcagtc
     4381 gcgctgacga ccgacacgtc ggcgttaacc gccgtcagta acgatctggg attcgaacag
     4441 gtcttcgcgc gtcaggtcgc cggactggcg caagcgggag acgtgttgat tgcgattagc
     4501 actagtggaa aatcgaagaa cgtgttaaga gcagtcgaac aggcgaagga attgggctgc
     4561 aagacaatcg cgctcacggg cgcgtcggct gagacgctct cgtccctgtg tgacgtagtc
     4621 gtaacagtgc ccgccacacg gacctcacgc attcaggaag ctcacatcac catcggtcat
     4681 ctctggtgcg agatggtcga cctgttctcc taggcacaag tcagacacaa cattttgtgc
     4741 tcaactatct tgacacctgc aagggattga actagtctct gctcgcacat ttttgaacgt
     4801 caaccgcccc tttggattgc gcggagcttc ccgacaggcg tcgccgcttg tgattcattg
     4861 agacgtttaa tggtttctag cgaactacct atcaacaaaa agtcaaactc cgcaaactca
     4921 tcgtcagcag tcgcgttaag cacccccgtc gaagattgtt ttttaacttc gaacggccga
     4981 aggtctgttt ctgacgcgct gtaaagtctt cgagggatga gttgagagtg ttttgaaaac
     5041 ctgctgctgc ctcgccagag aggcttcccg attgggtact ctgtgtgttc ggtcgtcgca
     5101 aagacgcaag gcacgcaaac gctgaggtgt gcattgatga ccatcaaacc acccacgcag
     5161 gggaaaaaga agaagcgcaa aaagaagacg gcactggtcg tctgtcgcga tcgaaagcag
     5221 ttctggacca cgcagacgca gttctggcag tgggtcaggg aaggaatcgt caccaaagtc
     5281 ggcgaccagc cgctgacggg aatgttccgc cgcgagcatg aggagttgat ggtgctgttg
     5341 agcaatacga ttctgaacct ggcgcaccca aatcacatgc gtgaagcttt gttgtcgcgg
     5401 cgcatcggaa tggcgggaaa ataagccacg gattgaacgg actggagtta ttgatgcgga
     5461 aggtcttagt gacagggatc ttactgatga tggcggtgag tgtgcacgcg cagcaggacg
     5521 atcgttaccg catcggtcca ggcgacgtgc tcgacatccg gatctacaac cgaccgcagt
     5581 tgtcgcgcga ggcggtgagg gtcgagggca gcggcatgat ccgcatgcca ctgattgaat
     5641 ccgagattca ggctgtgtgc ctcaccgaag gtgaactcgc gaaggagatc gccacgcgtt
     5701 atgcaaagta ttaccgtaac cttcaggtcg atgtctttat cagggagttt cactcgcggc
     5761 aggtcgccat catcggcgcc gttaacgatc aaagccgttt cgaactgcag cggcgcgtta
     5821 gcctgttgga actattgaca tacgccaaag gtccgtccac taaagccggc cagaccgtca
     5881 acatcgttca ctcgaccacg acctcaccgt gcaaacaagc taccgataac gaagacaccg
     5941 ccgcgctcac cagttacaaa ctcagcgacg tcctcgaagg taaaccggag tcgaatccct
     6001 atctcgaggc aggcgatatc gtaaccatac ccgaagcgga ccaggtctac gtcgtcggca
     6061 acgtgtttat gccgctgacg attccgctgc gcgaaccaat cacgttgacg cgtgcaatcg
     6121 cgatggccgg ggggctgaaa caggacacgc gcaaagacaa aattcgcgtg ttgcggcagg
     6181 aaccgggctc ctcggttcgc aaagagataa cggttgactt gtatgcaatc gagaagagac
     6241 gttccgaaga tctcgcgctc gcgcccaacg acatcatcga cgtgccgacc tcagccggca
     6301 agagtttcct gcgcagtttg attcaaggtg tggtgcccgg agtcggacag ttgcctgtcc
     6361 gagttgttcc ttgaaccttc gctacggatt acacagatga aacctcctac agaccttgtc
     6421 aaacaagagc ccgccgctgc tcttgaacgg gtcgaacggc cgcagtcgta caactacggt
     6481 gtcgatccaa acgcccagca cgaagttcat ctcatcgact actggcgagc gatccgcaaa
     6541 cgtatttgga tggtgctcgg gttggtggcg ctgattacga tgatcgccat cgtacacgtc
     6601 gcgcgtaagc cggattactt cgaatcccag gcgcgcgtgc aggtgaaatc ggaagacaac
     6661 tcgacactga cggggaagac gccatacttc tacagcgcga gtgaagattc gatttacttc
     6721 aatacacagc tccaaatcct gacctcgcag ggattgatgc ggcgagtagt caggactctc
     6781 gatctcgaac acaatccgga tttcttcaaa ggcagcaacg cacaaaagcg ttccacctgg
     6841 cgcacgatcc tcgaaatggc cggacttggt cgagatgcaa acacttccgc gtcacccgat
     6901 caactgccac tgacatcttc cgtagctccg gcaacgtcgc gcgaagactt aaccgaagcg
     6961 aaacgtctcg cgccctacgt cgcagcaatc ctcggcggtt taagagttga ccccgtcaag
     7021 gaggcgcgcg gttactacaa agagacgagt ctgatcgaca ttcgctactc acacaccgat
     7081 ccgcacgtgg cggcgaaggt cgtcaacgca attgccgaca cgtacgtcta cgcgaatctc
     7141 gaaaagaaaa tagagacaaa cagctcgacg ggaactttct tgcagaagcg cattgccgaa
     7201 ctgcaacagc agatccgaac tgatgaagag cgtctggtta actacgctcg caacaaccag
     7261 atcatctcgc tcgatccgac tcaaaacacc gtcgtcgaac gactcgcggg cttgaatcgg
     7321 cagttgttgg aagcggagaa cgagcgcaag acggcggaag cggcgtttac cgcagcgagt
     7381 gcgcccggag cggccgaggc gttggctgag aatgacgcga agcaagtatc ggatctggaa
     7441 accaaactta atgatcttcg gcaaaagcgc gcgcaactcc ttgtcgaagc gactgaagag
     7501 gcgccggaag tcaaggaagt ggaccagcag attgcggagc tgaacaagca attgaaggat
     7561 ctgcgaagcc ggaagtccag cgcgttgctt accacgctcg aaaccagata caagcaggcg
     7621 cttggtcgag aacaagcgct gcgcaaatca ttcgacgagc aaagagcgga aactctgacg
     7681 cagaatgaag ccgcgatcaa ccaccgcatt attcaacagg agatcgaaac caataagtcg
     7741 ttgctcaaca gtctgcttca gggcgcgaag gaaaatgacg tcgtaattgc cggaaagccc
     7801 aacaacattt cggttgtcga ttatggcctg gcgccggact ctccagtcgg accgaatcgc
     7861 acgcgaacca tcatcacagc tttcttcctc gcgatcggtt tgggagttgg gttcgcgctg
     7921 ttcctggaat atctcgacga cacggtacac acgaccgaag aggttgaacg tgggctgcaa
     7981 cttccggcgc tggctgtcat tccctctgtc ggagcgagag tctcgcgcaa tcggctgatc
     8041 gatacttcga aagcgctgtt gaagcgcaac ggaaacggcc acgtcgatag aagcgaggag
     8101 ttgttgat
//
LOCUS       DASTEY010000385         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_849110, whole genome shotgun sequence.
ACCESSION   DASTEY010000385 DASTEY010000000
VERSION     DASTEY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849110"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VFO72_06745"
     CDS             complement(<1..1299)
                     /locus_tag="VFO72_06745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9479023.1"
                     /translation="MTQFDLLIVGAGPAGSFAGELLAKRGAKVALFDGRPPGEPKACG
                     GGVTAKALKAWPDLLHAVGRTINQLDLYSPSGKRLHLKLDEPFAVYSRVVFDGYLRER
                     ARNAGAQVFSEKISARKITKTEAGWRLSSGAGEWSGSVLVGADGANSGIARKLAGPLS
                     PSDMEVAFGYRAPLPSSGAAPTIVAFLPRWVGYAWAFPRPDHISFGIATTQDAFEHQP
                     LDHLLWRFMIGYYRQQQGGKVNFWKDENGTNETAAIRDHLNATAERYAARIPGLSATT
                     WDSRTVGGDDWALLGDAAGFADPVTGEGIYYALRSAELFAEAYVRGDLRSYETSWRRD
                     FGAELRRAAQMRRRFYGNFWGAPFTERMIEFAKGHRGVKRVLGDLVAGEQGYVDLKKK
                     LVKSALRPLLFAVVGLAFVLTSSLHVRAQKVKQPKEVIAAY"
     gene            1428..>2283
                     /locus_tag="VFO72_06750"
     CDS             1428..>2283
                     /locus_tag="VFO72_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HET9479024.1"
                     /translation="MVPEVGRESIAGVRQSIEAMVELTERLIASGAEAVILISPHAPL
                     EIDAFVAYEGPELYGDFANFHAPETVVSAPVDDELLAAITASAASENYRVATLPDMDL
                     DHGTAVPLYFLLRNGWQGKVVALGYSFLSNDDHLRFGACIKRAVETVGRPVGFIASGD
                     LSHRLAPHAPAGFNPNAHVFDERVVEALRSNDPQAIVEIDYNLRKLAGECGYRSMLVA
                     IGAGSGLPLSCEVMNYEAPFGVGYLVAQLINQRSNLKASTADNLPGLAREAVETFIRT
                     GNVLEPPVR"
BASE COUNT          493 a          681 c          635 g          474 t
ORIGIN      
        1 ataggccgca atcacttcct tgggttgttt cactttttgc gctcggacgt gcaacgatga
       61 tgtcaacacg aaggccagac cgacaacggc gaacaaaagc ggtcgcaacg cgctctttac
      121 cagtttcttc ttcaaatcaa cgtaaccctg ctcacccgcc acgagatctc ccaacacgcg
      181 cttaactccg cgatggcctt tcgcaaactc gatcatgcgt tcggtgaatg gcgcgcccca
      241 aaagtttccg taaaaccttc ggcgcatctg tgcggcgcga cgcagttcgg cgccgaagtc
      301 tctgcgccaa cttgtttcgt aagatcgcag gtctccacgc acgtaagctt cggcaaacag
      361 ttccgccgaa cgtaacgcgt aatagatgcc ttcgccggtc acggggtccg cgaaaccggc
      421 agcgtcaccg agcaacgccc aatcatcgcc gcctacggtt ctgctgtccc acgtcgtagc
      481 cgagagacct gggattcgcg cagcgtagcg ttcggccgtt gcgttgagat ggtcgcgaat
      541 tgccgccgtt tcgttagtgc cgttctcgtc tttccagaaa ttaactttgc cgccttgttg
      601 ttgacggtag taaccaatca tgaaccgcca gagcagatgg tccagtggct ggtgttcgaa
      661 agcatcctgc gtcgtagcta tgccgaacga gatgtgatca ggacgcggaa acgcccaggc
      721 ataacccacc caacgcggca gaaacgcaac aatagtcggc gcggctccac tactgggcaa
      781 aggtgcgcgg taaccgaaag cgacctccat gtcggaaggt gaaagtggac cagccaattt
      841 ccgcgcaatg ccgctgtttg cgccatcggc accgacgagc accgagcccg accattcgcc
      901 ggcaccgctc gacaaacgcc agcctgcctc agttttcgtt atctttctcg cggagatctt
      961 ttctgagaag acctgcgcgc cggcattacg tgcacgctcg cgcaagtagc cgtcgaatac
     1021 aacacgtgaa taaaccgcga acggctcgtc gagtttcaag tgaagtcgct tgccggaggg
     1081 cgagtagagg tcgagctggt tgattgtgcg acctacggcg tgcagcaaat caggccacgc
     1141 cttcagtgcc ttcgccgtca caccgccacc gcaggccttt ggctcacccg gcggtcgtcc
     1201 gtcgaaaaga gctacttttg ctccacgctt cgcgagcagt tcgccagcga acgaaccggc
     1261 cggaccggcg ccaacaataa gcagatcaaa ctgagtcatg gaattgaatt gtgaccctcg
     1321 gctgacagag ctagaataca gccgtccctc tcaaaccgcg aaagcgcacg atgaccaacc
     1381 aaagcagttg cctagttttc tccggcattg caccacatcc gccgattatg gtgccagagg
     1441 taggccgcga gtcgatagcg ggcgtgcgcc agtcaatcga agcaatggtc gagctcactg
     1501 agaggttaat cgccagcggc gccgaggctg tgattctgat ctcaccgcac gcacctctcg
     1561 agatcgacgc cttcgttgct tatgaagggc cggaacttta tggcgacttc gcgaactttc
     1621 acgcgccgga aaccgttgtg tcggccccgg ttgatgatga actactcgct gcgattaccg
     1681 cttccgccgc aagtgaaaac tatcgcgtcg cgaccttgcc cgacatggac ctggaccatg
     1741 gcaccgcggt tccgctttac tttctgttgc gaaacggatg gcagggaaag gtcgtcgcgc
     1801 ttggttacag cttcctctcc aacgacgatc atctccgctt cggcgcctgc attaagaggg
     1861 cggttgaaac tgttggccgg ccagtggggt tcattgccag cggcgacctg agtcaccgcc
     1921 tcgcgccaca tgctccggcc gggttcaatc caaacgcaca cgtgttcgat gagagagttg
     1981 tcgaggcttt gcggtctaac gacccgcaag caatcgtcga gatcgattac aacctgcgaa
     2041 aactcgccgg cgaatgtggg tatcgctcga tgctggttgc aatcggcgct ggttccggac
     2101 tgccgctgtc gtgcgaagtg atgaattatg aagcgccatt tggcgtggga tatctcgtgg
     2161 cgcagttgat aaatcaacgc tcgaatttga aagcgtctac agcggataat ttaccgggcc
     2221 tggcgcgaga ggccgtggag acgtttattc gaaccggaaa cgtcttggag ccaccggttc
     2281 gaa
//
LOCUS       DASTEY010000386         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_502798, whole genome shotgun sequence.
ACCESSION   DASTEY010000386 DASTEY010000000
VERSION     DASTEY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502798"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /locus_tag="VFO72_06755"
     CDS             <1..1812
                     /locus_tag="VFO72_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9479025.1"
                     /translation="AHLASRLLFGLPLPRMNGQSAYKRVFALSLVVVLIGLLSAPLTL
                     AQRRPLPTTTTTAQTRRPRLVLLIVVDQFRYDYLERFGDLFGPNGLRRLLRDGASWSQ
                     ANYDHTPTYTAPGHGTMMTGAYPAESGIVGNEWFDRASGKRVTSVSDDTVKLLGGDAT
                     ETAASPRRLLSSTVGDELRLVTNDRSKVIGISVKDRSAILPAGRHANAAYWFSVRSGT
                     MVSSTYYFNELPTWVTNFNHTKPADKYFGAKWERLVAENEYVKRAGPDSPSWETVGSA
                     SGDTNAFPHTITGGATTPGRAFYNALDYTPFSNDLLVSFAQQAIVSEQLGEDEDTDVL
                     TVSFSANDYVGHRYGPYSQEAMDVTLRVDRQIATLLDFVQGRGGLANTLIAFTADHGV
                     APIPEQAATLGLGGGRIKTADILARLRTAISARYNPQGRSPDPSADYILKASNFGTVS
                     DVFINGNVYFNYEALKRDGVDLDEISRVVAVAAQTIPGVSRSFTRAQLLRGATSISDP
                     IERRVLHGFSPARSGDVVMIAEPFKYLGDTITATHGSPYSYDTHVPVIIMGAGIMPGR
                     YLEPASPADIAPTLSALLRVTAPTSTTGRVLTEAIRK"
     gene            1809..2561
                     /locus_tag="VFO72_06760"
     CDS             1809..2561
                     /locus_tag="VFO72_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9479026.1"
                     /translation="MTEQKREGFHSVIPYLTVHKPGELIDFVKYSFGAIEHLRTTGSA
                     GGMHAEVKIGDSIVMIGGAEHITPAPAAIHLYVPDVDAAFRRAVEAGGKSLFEPIDQS
                     YGERSGGVEDPTGNRWYIATTVVPLREISEHLHTVTVFFHALGAAKFIEFLTTAFEGE
                     ELMRTEDNGRIAHAKVRIGDSAVELGEAREQWQPLPSAIYLFVADVDATYERALRAGA
                     TSMMEPKDQPYGHRNAWVKDPFGNVWYLAREI"
     gene            complement(2558..>2921)
                     /locus_tag="VFO72_06765"
     CDS             complement(2558..>2921)
                     /locus_tag="VFO72_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9479027.1"
                     /translation="FHHSMNYRSVLVFGRATLVDDPKEKYEALVALSEHIVRGRWPDV
                     REPNEQEMTMTTVLCLPLEEASAKIRTGPPLDDEEDYDLPVWAGIIPLKLVAGEPIKD
                     PRLPDGIGVPEYAAHYKR"
BASE COUNT          641 a          861 c          807 g          612 t
ORIGIN      
        1 gcccatttag cctccaggct attgttcggg ttaccattgc cgcgcatgaa cgggcaatcc
       61 gcttacaaac gtgtattcgc tctatcgctg gtagttgtgc taattggcct gctgagtgct
      121 ccacttacat tggcccagcg acggccgctg ccaacaacaa ctacaactgc tcaaaccaga
      181 cgccctcggc tcgtgttgct gatcgtggtg gaccagtttc gttacgatta cctggaacga
      241 tttggtgatc tgtttggtcc aaacggcctc cggcgtttgt tgcgcgacgg cgcgtcatgg
      301 tctcaagcga actacgacca cacgccaact tacacggccc ccggtcacgg cacgatgatg
      361 accggcgcct atccggccga atcgggcatc gttgggaatg aatggttcga ccgcgccagt
      421 ggcaaacgcg tcaccagcgt ttcagacgac actgtcaaac tgctcggcgg cgatgcgact
      481 gaaaccgcgg cgagtccgcg gcggttgctc agttctacag tcggtgatga actacgcctc
      541 gtcacgaacg accgttccaa agtaatcgga atctcagtga aggatcgctc ggcgattctg
      601 cccgccggac gtcatgccaa tgctgcctac tggttcagtg tcagatccgg cacgatggtg
      661 tcttcgactt actacttcaa cgaactacct acgtgggtta ctaatttcaa ccacaccaaa
      721 cccgccgata agtacttcgg cgcaaaatgg gaacgtctcg tcgcggaaaa cgaatacgta
      781 aaacgtgcgg gtccggacag tccgagttgg gaaacagtcg gcagcgcaag cggcgacacg
      841 aacgcgtttc cccacaccat cacgggcggc gccactacac ccggacgagc gttctacaac
      901 gctctcgact acacaccatt cagcaacgat ctactcgttt cattcgccca acaggcaatc
      961 gtcagcgaac aactcgggga ggacgaagac accgacgttc tcaccgtcag cttttccgcc
     1021 aacgattacg tgggccatcg ctatggaccg tatagccagg aagcgatgga tgtgacgctg
     1081 cgtgtggatc gccagatcgc aaccttgctc gactttgtgc aaggtcgtgg cggtttggcc
     1141 aacacattga tcgcgttcac tgcggatcat ggtgttgcgc cgattcccga acaggccgcg
     1201 actctcggcc tcggcggtgg gagaatcaag acagccgaca tcctcgccag actccgaact
     1261 gcgatcagcg cgcgctacaa tccacagggc aggtcgccgg atccgagcgc agattacatt
     1321 ctcaaagcca gcaacttcgg aaccgtgagt gacgtgttca tcaacggcaa cgtctatttc
     1381 aactacgaag cactcaaacg cgacggcgtc gatctggacg agatctcaag agtggttgcc
     1441 gtagcagcgc agacgattcc gggcgtctct cgctcgttta cacgtgccca actcctgcgt
     1501 ggcgcaacgt cgatcagcga tccgatcgaa cgccgcgtgc ttcacggttt ttctcctgca
     1561 cgcagtggcg acgtagtaat gatcgccgag cctttcaaat atctgggcga cacaatcacc
     1621 gcgacgcacg gttcgccata ttcctacgac acgcacgtgc cggtgatcat catgggagcc
     1681 ggaattatgc cgggccgtta tctcgagccc gcgtctccgg ccgacatcgc gcccacatta
     1741 tcggccctgc tacgcgtcac cgctcccacg agtacaaccg gccgcgtact gacggaggca
     1801 atcagaaaat gacggaacag aaacgcgaag gatttcatag cgtcatcccg tatctgacag
     1861 ttcacaagcc cggggagtta atcgatttcg tcaaatattc cttcggcgcg atcgaacatc
     1921 tccgcacgac cggttccgcc ggcgggatgc acgctgaagt aaaaatcggc gactcgatcg
     1981 tgatgatcgg cggcgccgaa cacatcactc cggcgcctgc ggcgattcat ctgtatgtgc
     2041 ccgacgtgga cgcagctttc cggcgcgcgg tcgaagccgg cgggaagtcg ttgttcgaac
     2101 cgattgacca atcttacggc gagcgttcgg gcggagttga ggatccgacg ggcaatcgct
     2161 ggtatatcgc gacgactgtt gttccgttgc gtgagatttc ggaacatctt cacacggtca
     2221 cagtattctt tcacgcgctt ggcgcggcga agtttattga attcctgacg actgcgtttg
     2281 agggtgaaga gttgatgcgc accgaagaca atggacggat cgcgcatgcg aaagtgagaa
     2341 ttggcgattc cgctgtcgaa ctcggcgaag cccgcgagca atggcagcca ttgccaagtg
     2401 cgatctacct ttttgtggcg gatgtcgacg cgacttacga gcgagcgctg cgcgccggtg
     2461 ctacttcaat gatggagccg aaggaccaac cttacggtca tcgaaacgcg tgggtaaaag
     2521 atccgttcgg gaacgtttgg tatctggcgc gtgaaatcta gcgtttatag tgcgccgcat
     2581 actcaggaac gccaatcccg tccggtaatc gtggatcttt aattggctct cccgctacga
     2641 gcttcaacgg aatgatccca gcccacacgg gcaggtcgta atcttcttcg tcatcgagcg
     2701 gcggaccggt gcggatcttt gccgacgctt cttccagcgg caggcaaagc actgtagtca
     2761 ttgtcatttc ctgctcgttc ggctcgcgaa cgtcgggcca gcggccacga acgatgtgtt
     2821 cagacaaagc gacaagcgcc tcgtattttt ctttcggatc gtcaacgagc gtggcgcgtc
     2881 caaacaccag caccgaacga tagttcatcg aatgatgaaa c
//
LOCUS       DASTEY010000387         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_667772, whole genome shotgun sequence.
ACCESSION   DASTEY010000387 DASTEY010000000
VERSION     DASTEY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667772"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1032
                     /locus_tag="VFO72_06770"
     CDS             157..1032
                     /locus_tag="VFO72_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479028.1"
                     /translation="MHVSIFIPLFTLAALLPLPLAATQTTKPAAAAPQIQLLTRSTRR
                     SESRHFSFGGTVTLIGAPRGAVTVEGWSRNIVELTADIELKAQNEADLDQLAKVNNFV
                     FDEDMNHINILTVGTHDRSYMKKVAKNFPKKLLNLPWKIDYRLRVPANTDLEISAGHG
                     PLKLSGVEGALRISATESETALTLTGGTVSATITAGNILLSIPVRSWRGGGADIRVAA
                     GVLNVELAPGFSGDIDADILRSGKIENTYDALAAREKPGITGRTVRARAGAGGAFFKF
                     TVGDGTVNIRRAGDK"
     gene            1029..1595
                     /gene="dcd"
                     /locus_tag="VFO72_06775"
     CDS             1029..1595
                     /gene="dcd"
                     /locus_tag="VFO72_06775"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET9479029.1"
                     /translation="MSIKSDRWIRMMATEYGMIEPYEAEQVKQVDGQRIVSYGTSSYG
                     YDIRCADEFKLFTNINSTIVDPKNFDEKNFVDIKGDSVIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGILVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVVFF
                     ESDDVCETSYKDRGGKYQGQRGVTLPKM"
     gene            1620..>2053
                     /gene="dut"
                     /locus_tag="VFO72_06780"
     CDS             1620..>2053
                     /gene="dut"
                     /locus_tag="VFO72_06780"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET9479030.1"
                     /translation="MTTPLDVLRFKQLDPKATLPVRGSSFAAGLDICSVEDLVIEPYQ
                     RTTARTGLAVAIPHGFYGRVAPRSGLAAKHGLDVLAGVIDSDYRGELCCVLYNTGDAA
                     INLPAGSKICQLIVEKIITPQATWVNDLDETARGAGGFGSTG"
BASE COUNT          483 a          585 c          561 g          424 t
ORIGIN      
        1 ccgtttcagt aacaaatttt agtggtcaga tcactttctt gcggaaataa actccgcagt
       61 tgcaatggca agcccggatg cggacgttgc gtcagcaacg tccgtttttt tattttgcat
      121 tctgtgaaag gacgcttact attgggcggc ctccttatgc acgtttcaat tttcatcccg
      181 cttttcacgc tagctgctct tttgccgttg ccgttggctg caacgcaaac gactaaaccc
      241 gctgctgccg cgccccagat ccaacttctg acgcgcagca ctcgacgcag cgaaagccgc
      301 cacttctcct ttggcggcac agtgactttg atcggggcgc cgcgcggggc cgtcactgtc
      361 gaaggctggt cgcgcaacat cgtagaactg acggctgata tcgagctgaa agcccaaaac
      421 gaagcggacc tggaccagct cgcgaaggtg aataacttcg tcttcgacga agacatgaat
      481 cacatcaaca ttctcaccgt cggcacacac gatcgttcct acatgaagaa agtagcgaag
      541 aattttccaa aaaagctgct gaacctgccg tggaagatcg attaccggct gcgagtgccc
      601 gccaacacgg atttggaaat cagtgccggg catggtccat tgaaactttc cggagtggaa
      661 ggcgcattgc ggatttcggc cacggagagt gaaactgcct tgacgctgac cggagggacc
      721 gtatcggcaa cgattactgc cggaaacata ctgctcagca ttccagtccg cagttggcgc
      781 ggcggcggcg ccgacatccg tgtcgctgcc ggcgtcttga acgtcgagct ggcgcccggg
      841 tttagtggcg atatcgatgc cgacatcctg cgcagtggca agatcgaaaa tacttacgac
      901 gcgctcgcag cccgcgagaa acctggaatc acgggacgca ctgtgcgcgc gcgagcaggc
      961 gccggcggag ctttttttaa attcacagtg ggagacggaa cggtgaacat ccggagggct
     1021 ggggacaaat gagtataaag tcggatcgct ggatacgaat gatggccact gaatacggaa
     1081 tgatcgagcc atacgaagcg gaacaggtga aacaggttga cgggcaacgc atcgtatcct
     1141 acggcacgtc cagttacggc tacgacattc gctgcgctga cgagttcaaa ctcttcacca
     1201 acatcaacag caccattgtc gatccgaaga atttcgacga gaaaaacttc gtcgacatca
     1261 aaggcgattc agtaatcatt ccgccgaata gtttcgcact cgcgcgtaca gtcgagtact
     1321 ttcgcatccc gcgcaacgtg ctgacaatct gtctgggcaa gagcacctat gcacggtgcg
     1381 gcattctggt gaacgtgacg ccgttcgaac ccgaatggga aggttacgtc acgctcgagt
     1441 tttcaaacac cactccgtta ccggcaaaga tctacgcgaa cgagggcgtc gcacaagttg
     1501 tgttcttcga atctgacgac gtttgcgaga cgtcgtacaa agatcgcggc ggcaagtacc
     1561 agggccaacg cggcgtcacc ctgccgaaga tgtgaaccac agatataacg gagttacgga
     1621 tgacaactcc tctggacgtg cttcgtttca agcaactgga tccaaaagcg actcttcctg
     1681 tccgtggcag ttcgttcgcc gcgggtctcg acatctgctc tgtcgaagat ctcgtgattg
     1741 aaccatatca gcggaccacg gcgaggactg gtctcgccgt tgctatcccg cacggattct
     1801 atggccgcgt ggcgccacga tccggactcg ccgcgaaaca tggactcgac gtgctcgccg
     1861 gcgtgatcga ctccgactat cgcggcgaac tctgctgtgt gctctacaac acaggcgacg
     1921 ctgcgatcaa ccttcccgcc ggaagcaaga tctgccaact catcgtcgag aagatcatca
     1981 cgcctcaagc cacttgggta aacgacctcg acgaaactgc cagaggcgcc ggcggcttcg
     2041 gttccacagg cta
//
LOCUS       DASTEY010000388         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_255863, whole genome shotgun sequence.
ACCESSION   DASTEY010000388 DASTEY010000000
VERSION     DASTEY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255863"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFO72_06785"
     CDS             <1..711
                     /locus_tag="VFO72_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9479031.1"
                     /translation="ATEGASLVLCARDAATLAETTASIADQTGAHVLAVPADVTDAQQ
                     VKNLVASGIDRFGRIDILVTNAGGPPAGRFDQLTQEQWERATQLTLFSAVHLAREVLP
                     GMKARHWGRILNITSIAVKHPVENLLLSNSLRAAVTGFARTLANEVATEGITVNNILP
                     GYTRTERLEELASMMAEKQGISANEFRAKWEAEIPIGRLGEPREFAAVAAFLVSERAS
                     YITGTSIQVDGGWIRSLF"
     gene            742..1395
                     /locus_tag="VFO72_06790"
     CDS             742..1395
                     /locus_tag="VFO72_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9479032.1"
                     /translation="MAAPSDNSIEQSSQNSLDLTGDLTGEGVIIDIGTGDGRFVYQSA
                     RQNPKKFYIGIDPNVRPLAKISEKTYRKPSKGGAPNVLFVQAAVEDLPAELDGVADEV
                     HVHFPWGSLLRAVATGDVVVLRNLRRICSAGALLEVVMGLDPLRDQSEIERLGLEPLT
                     VAYIDEILASNYAAAGFEITERGIIAASEWPEFNTSWAKRLQQGEQRTITYLISRAV"
     gene            1493..2023
                     /locus_tag="VFO72_06795"
     CDS             1493..2023
                     /locus_tag="VFO72_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET9479033.1"
                     /translation="MARSAAGKATSQRALSDEISKDNKPTSDVVEHLQRQLANAFVLY
                     ANYKHYHWQTFGPLFRDLHLLFDEFATAVLGTIDDFAERVRMIGQDPVFRPDEVERLA
                     SVKVSNDAGNMREMVAEANTNLLVVIKEMREAARVADDHDDPGTVDLFSRHVQIHEKH
                     EWWLRDILEKGDGLST"
     gene            complement(2192..2701)
                     /locus_tag="VFO72_06800"
     CDS             complement(2192..2701)
                     /locus_tag="VFO72_06800"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9479034.1"
                     /translation="MKLTEFFREELDREVERSRKALEQVPSGKYDWKPHDKSMIFGYL
                     ADMVATIPMWIQMEIERDELDVAPPEGQGMKPPPKETSEALIKALDEAAAGARAALEK
                     TTDEHLRTNWKLLAGGQVVLEEQRYMMIQETINHWAHHRGQMTVYLRLMGQKVPALYG
                     PSADEQSFK"
BASE COUNT          673 a          792 c          822 g          632 t
ORIGIN      
        1 gcgaccgaag gagcttcgct cgtgctctgc gcccgcgatg cggcaacgct cgcggaaacc
       61 acagcctcaa tcgcggatca aaccggagca cacgtgttag ccgtgccggc ggatgtaacg
      121 gacgcacaac aggtgaaaaa cctcgtcgcc tcagggatcg accgatttgg ccgcatcgat
      181 attctggtga cgaacgccgg tggaccgcca gccgggcgtt tcgatcagtt gacgcaggag
      241 cagtgggaac gagccacaca attgacgctc ttcagcgcgg ttcacctcgc acgcgaagtt
      301 ttgccgggaa tgaaagcacg gcattgggga cgaatcctga acattacctc gatcgcggtg
      361 aaacatccgg tcgagaatct cttgttgtcg aacagtctgc gcgccgcggt caccggcttc
      421 gcgcgcacgc tggcgaatga agtcgcaaca gagggaatca cagtcaacaa catactgccc
      481 ggatatacgc gtactgagcg cctggaagaa ctcgccagta tgatggctga gaagcaggga
      541 atcagtgcca atgagtttcg cgcgaagtgg gaagcagaga tacccatcgg acgtctcggc
      601 gagccgcgcg agtttgcggc ggtcgccgcg tttttagttt cggaacgggc gagttacatt
      661 accggtacgt cgatccaggt cgacggcggt tggatccgat cattgtttta gaaagggcga
      721 caatcatgat caaggcaatt cgtggcagca ccaagtgata actcgataga gcagagttcg
      781 cagaactcat tagaccttac cggcgatttg accggcgagg gagtgatcat cgatatcggg
      841 acgggcgacg ggcgctttgt ataccagtcg gcgcgccaga acccgaagaa gttttacatc
      901 gggatcgatc ccaacgtccg gccgctcgcg aagatctcgg agaagaccta ccgtaaaccg
      961 tctaagggcg gcgctcctaa cgttcttttc gttcaagctg cggtggaaga tctacctgcg
     1021 gaactggacg gtgtggccga cgaggtgcac gtccatttcc cgtggggcag tttgttacgc
     1081 gccgtcgcaa caggtgatgt tgtcgtgttg cgcaacctgc gccgcatctg ttcggccggt
     1141 gcgttgcttg aagttgtgat gggactcgac ccgctgcgcg atcagtccga aatcgaacgt
     1201 ctgggtttgg agccgctcac cgttgcatac atcgacgaga tccttgcgtc caactacgca
     1261 gcggcgggat tcgaaatcac ggaaagagga atcattgctg cttcagagtg gcctgaattc
     1321 aacacctcct gggcaaaacg gctccaacaa ggtgaacaac gtacaattac ctacctcata
     1381 agccgcgccg tctaaatggt cctctaaaac gtcggacatt tcccgcagac aatcgacacg
     1441 ttttcgccaa caccagtgtg ttgaatgcca gtgaaaaggg agggttttaa atatggcgag
     1501 aagtgctgca ggaaaagcta catctcaacg cgcgctatct gacgagatct cgaaagacaa
     1561 caagccgacc agcgacgtcg ttgaacacct gcaacggcaa ctggcaaatg cgtttgttct
     1621 ctacgcgaat tacaagcact atcactggca gacgtttggg ccactgtttc gcgatctgca
     1681 cctgttgttc gacgagtttg caaccgcggt gttgggcacg atcgacgact tcgcggaacg
     1741 cgtgcggatg atcggccagg atccggtctt tcgtcccgac gaagtcgagc gactcgcgag
     1801 tgtgaaagtg tcaaacgatg ccggcaacat gcgcgagatg gtggccgaag cgaatacaaa
     1861 cctgctggtc gttattaaag agatgcgcga agctgcgcgt gtggcagacg accacgacga
     1921 cccggggacg gtcgatctgt tttcaaggca cgtgcagatt catgaaaaac acgaatggtg
     1981 gctgcgcgat atcctcgaaa aaggcgacgg tttatctact taaagcgggt ttgcacgaaa
     2041 agtacctaaa tggcgtttga ggaaccacca ctgtggaaaa aattgtttcg cgcaaaggcg
     2101 caaaggcgca aagaaaaccc ttataaacgc ggtagccgct ttgcgccttt gggcgagagt
     2161 tctttcagaa agatgtactt ttcgcgcaaa actacttaaa gctttgttcg tcggcagatg
     2221 gcccatacag cgccgggacc ttttggccca tgagacgcag gtagacggtc atctgcccgc
     2281 gatggtgcgc ccagtggttg atcgtctcct ggatcatcat gtagcgctgc tcttcaagca
     2341 ccacctgtcc gcccgcgagt agtttccagt tggtccgcag atgttcgtcc gtggtctttt
     2401 cgagcgccgc gcgagcaccc gccgcggctt cgtcgagcgc tttgattaac gcttcgctcg
     2461 tctctttggg cggaggtttc atgccctgtc cttcaggtgg cgctacgtca agttcgtcgc
     2521 gttcgatctc catctggatc cacatcggaa tcgtcgcaac catatctgcg agataaccaa
     2581 aaatcatcga cttgtcgtgt ggcttccagt cgtacttgcc gctgggcact tgctccagtg
     2641 ctttgcgcga acgttccact tcccgatcga gttcctcgcg aaaaaactca gtcagcttca
     2701 tcgttgccct ccagatccgt actatgattt tttgccgacg atatgagaat ctaacaacca
     2761 gacgccagtc ggcacaaacc gggattgctt tgcctcaaaa gtcctctctc attctcaccg
     2821 ggcttttagc ccggtgcagg agcagtgaag acggaggaaa ccgtttcaac ggttttccgt
     2881 tgattcgcca gcgaaaccgt taaaacggtt tctcaaaac
//
LOCUS       DASTEY010000389         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_915181, whole genome shotgun sequence.
ACCESSION   DASTEY010000389 DASTEY010000000
VERSION     DASTEY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915181"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VFO72_06805"
     CDS             <1..1138
                     /locus_tag="VFO72_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET9479035.1"
                     /translation="VVQGIAPARNAIVTGRVDWSRRLDHMQQHTGQHILSQALVKLFN
                     APTRSFRVLEMTCEIDVELGNPTTEIIERAVELANNVVWEDRAITIHNVSSEEAAAMP
                     LRKEPAREGELRLIEIEGFDLTPCGGTHAYRTGEVGMIAMRSWERAKGLTRIEFVAGG
                     RALVDYRRANRSARAVAALFSTGRDDAPQHAAQLADENKELHRRVRALEELAAGVEAE
                     RLLASAVVRDDGTRVVTCVFDAHDDREAREDREAREDREVREDRDHSSEPSTDTKPRA
                     VDSGREAKFFPHDAEFLKKLAHALIGHPQTIALLASRDKDAARLIFARSADAPGDMNT
                     LMREACTILDGRGGGKPGLAQGGGKNVTRLSEALERVAQSLADA"
     gene            1220..1296
                     /locus_tag="VFO72_06810"
     tRNA            1220..1296
                     /locus_tag="VFO72_06810"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1254..1256,aa:Ala,seq:cgc)
     gene            1951..>2160
                     /locus_tag="VFO72_06815"
     CDS             1951..>2160
                     /locus_tag="VFO72_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479036.1"
                     /translation="MSSIADVTAEASKLPDIPQRVKVLIEAAKILQPAKEEETIRLLE
                     VILRDLKEWASADDASWQQRSMSATL"
BASE COUNT          490 a          565 c          649 g          456 t
ORIGIN      
        1 cgtcgtgcag ggaatcgcgc cggcgcggaa tgcgatcgtg acggggcgag ttgactggtc
       61 gcggcgcctg gaccacatgc aacagcacac cggccagcac attctgtcgc aggcgctcgt
      121 aaaactcttc aacgcgccga cgagaagctt tcgcgtgctg gaaatgacgt gcgagatcga
      181 cgttgagctt ggcaacccaa cgactgagat cattgagcgt gcagttgaac tcgccaacaa
      241 cgtcgtgtgg gaagaccgcg cgatcacgat tcacaacgtc agttctgagg aagctgcggc
      301 gatgccgttg cggaaagagc ccgcgcgtga gggtgagttg agattgatcg agatcgaagg
      361 tttcgatctg acgccttgcg gcggaacgca cgcgtatcgc acgggtgaag tggggatgat
      421 agcgatgcgt tcgtgggagc gagcgaaagg attgacgcgg attgagttcg tggcgggcgg
      481 gcgcgcgctt gtggattacc gaagggcgaa cagaagcgca cgtgcggtgg cggcgctatt
      541 cagcaccgga cgcgacgacg caccgcagca tgcggcgcag ttggctgacg agaacaagga
      601 gttgcatcgc cgggtgcggg cgctggaaga gcttgccgcc ggcgttgaag ctgagcggtt
      661 gttggcgtcg gcggtggttc gagacgacgg tacccgtgtc gtaacgtgtg tgtttgatgc
      721 tcacgatgat cgtgaagcgc gcgaagaccg tgaagcgcgc gaagatcgcg aagtccgtga
      781 agaccgtgac catagcagtg agccgtcaac tgatactaaa ccgcgagctg ttgactcagg
      841 tcgcgaagct aagtttttcc ctcacgatgc tgagttcttg aagaagctcg cacacgcact
      901 gattggtcat ccacagacga tcgcgttact cgcttctcgc gacaaggacg ccgcacgcct
      961 gatcttcgcc cgctcggctg acgcgcccgg cgacatgaac acattgatgc gcgaagcttg
     1021 cacgatttta gatggtcgtg gcggcggcaa acccggcctc gcgcaaggtg gcggcaaaaa
     1081 cgtcacgcgc ttgagcgaag cgctcgaacg cgtcgcgcaa tcattagccg atgcatgacc
     1141 agtagcctag gcatgacact ccttaacccg ggaccagctt gacactcatc aactcgcttc
     1201 ctaatatggc aacccgcgtg gggcagtagc tcagtttggc agagcgccgc gttcgcaatg
     1261 cggaggtcag gggttcgatc cccctctgct ccaccatttg ttttcaatca tttacggctg
     1321 ccagcactgg cggccgtatt tcatttgtgg ccaatttgtg gacacttttt cgggagctca
     1381 gtttgatcgc ttcaacggct gcgcgtttcg tttcttcaac cgacctcaca taacgcatag
     1441 tcacgcgcac atccgagtgg cccaacagtt gagcaatcgt gaacgcatcg catccggcct
     1501 ccgctaagcg aacctgactg cgccaacgca ggcttcacag aaaggataat ccaatgcgtc
     1561 tcactaagac ttcttgcgct aatcttgttg cttgctattg gcacttttgc ctattttggt
     1621 tccacgacaa ctatggccga agacaccgct accaagcttg cagcggtgga tgcggattaa
     1681 atgcgagctg tctcaaaaag tgtgaggagg aaaggatgaa gtgtgaggcc agttgcaaat
     1741 agactagacc aaagttcttg cgggcacgtt ggcgcaccac atgcttgaaa cgtgccgaaa
     1801 agtatcaagg attgtaagga aagaggcatg caaacttcta aggttctatc gttagttgtc
     1861 ctgatttcgc tgagctttgg ctggccgcga tcactgtgta aagctcagaa aaagatctct
     1921 ccggctgatt atgatttcta tgctctcgca atgtcctcga ttgcagacgt aaccgctgag
     1981 gcgtcaaaac tgccggacat cccgcaaaga gtcaaagttc tgattgaggc tgcaaagatt
     2041 ttgcaaccgg caaaagagga ggaaacgatt cgcctgcttg aagttatcct gcgcgatcta
     2101 aaagagtggg cttccgcgga cgacgcgagt tggcagcaac ggagtatgtc ggcaactttg
//
LOCUS       DASTEY010000390         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1343523, whole genome shotgun sequence.
ACCESSION   DASTEY010000390 DASTEY010000000
VERSION     DASTEY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343523"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..348)
                     /locus_tag="VFO72_06820"
                     /pseudo
     CDS             complement(1..348)
                     /locus_tag="VFO72_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            complement(388..1377)
                     /gene="thiL"
                     /locus_tag="VFO72_06825"
     CDS             complement(388..1377)
                     /gene="thiL"
                     /locus_tag="VFO72_06825"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET9479037.1"
                     /translation="MSRSEFEFIDSLRRRFGAGIGDDAAVLRNTPNKEIVITTDLLAE
                     DIDFRRTTAPPLLLGHKALAVSLSDIAAMGARPRWSLISIGVPEDVWQTEFVDQFYEG
                     LASLAQRYDVQLIGGDTSRTHEKIVVDSIVLGECSAGHAVKRAGAQPGDQIFVTGSLG
                     GAAAGLRLIERGAHLAEQNLADDDSQKLDHVLLRQLRPEPRVGWGIVLGEERLATSMI
                     DISDGLSSDLNHLCAASGAGALIESSLLPIDHQVVELCGRRALDPLQLALHGGEDFEL
                     LFTVRAGDVARLPRRVDGTQITRIGEVRVHTEGVRISEGSRIWQLSPSGWKHF"
     gene            complement(1380..2549)
                     /locus_tag="VFO72_06830"
     CDS             complement(1380..2549)
                     /locus_tag="VFO72_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9479038.1"
                     /translation="MTTSPTVLRLASRLTEVGFSDIVKLRNRIMELREQGATVYQFEG
                     GEPFMPTPEPIKEAMTRALAENKTRYAPSSGIPELREAIAEKLRTRNRIPAEAKNVIV
                     VNGGMQALFGAFQSVVNPGDEVLLFSPYWTPIKDLVAHCQGRIVLVPTDEARQAGFQQ
                     TLARYATARTKAIYYNTPQNPNGIVLTPNEANAVASFVQERDLVVIADEAYEDLVYDG
                     EHCSIASLDGMFERTISCFTFSKSYAMTGWRLGYAVATEPWMTGLKKATLYSSNGVST
                     PTQWAGVAALNMDPALLDRARDEYRVRRDLLLSGLNEIGLTCARPAGAFYAFPDARLI
                     HPDSREAADILLNRAQVATVPGIVFGVDGEGYLRFSFSTSIETIEAGLDSLRRNL"
     gene            2924..3394
                     /locus_tag="VFO72_06835"
     CDS             2924..3394
                     /locus_tag="VFO72_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9479039.1"
                     /translation="MSRPAVAIPEKLFFKIGEVCELAGVQAHVLRYWESEFPMLAPQK
                     NRAGQRVYRKRDVEMALRIKELLYEDQYTIAGAKKRLTNELRGGGKLKVVGEEDGQAP
                     NGSAESESFTPANSSYASSVAPLAAEERQGLKQLAAELRELLSLLEQETKGQAK"
     gene            3493..3569
                     /locus_tag="VFO72_06840"
     tRNA            3493..3569
                     /locus_tag="VFO72_06840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3527..3529,aa:Pro,seq:ggg)
     gene            3693..4154
                     /locus_tag="VFO72_06845"
     CDS             3693..4154
                     /locus_tag="VFO72_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479040.1"
                     /translation="MSIFLLAVLAVVLICLALSTTAFVLVTGFILLVTLMSVYDRLRL
                     RRLAEQRMGESICTFVRSFDRHVVDPWVIRAMYEEFHAYFDGALPIRAADRIEEDLRM
                     DWDDVDDLLSDVALRAGRSLDRIESNPLYGQLRTVADLVLFLMHQPKCGAA"
     gene            complement(4306..4770)
                     /locus_tag="VFO72_06850"
     CDS             complement(4306..4770)
                     /locus_tag="VFO72_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9479041.1"
                     /translation="MSEVIVKSQGNLRNEVRYGAGHSFTTDEPVAAGGEDAGPDPYTL
                     LLAALGSCISMTVTLYARRKQWPLESVSVRLRQNRIHAKDCKDCQQTEGYIHRIERAV
                     TVTGPLTEEQHARLQEIAHKCPVHKTLTSAIVVTELKDDETSHDSEPSEGWF"
BASE COUNT         1056 a         1344 c         1384 g         1106 t
ORIGIN      
        1 acgagtggca cgcggcacga tgtccggtcg ctcgcaaccg agcgcatcga agtcttccca
       61 gagcgcgtcg atgtacttgg ccgtgtactc gtcaatgctc aacccggcct cttgcgactt
      121 cgcgatgatg cggtcttcga cgtccgtgat gttcatcacg tgcgtgacct tctaaccttt
      181 gaacttcaga taacggcgga ggacgtcggc ggcaacggcg ctgcggaagt ttccgatgtg
      241 gccgtaatcc cagacggttg gaccacagta gtataaccgc acctcacctt cgttgatggg
      301 gcgaaactct tcgatgcggc ccgatagcgt gttacgaaat ctcagcatga gttcattgcc
      361 gattttcgat tgccaattgc caatttgtca aaagtgcttc cagccgcttg ggctgagctg
      421 ccaaatcctg gacccttcgg aaatcctgac gccttcagta tgaactcgaa cttccccgat
      481 ccgggtaatc tgtgttccgt caaccctgcg cggcagacgc gcaacgtcac cggctcgcac
      541 cgtaaacaac aattcaaagt cctcgccgcc gtgcagcgcg agttgcagcg gatcaagcgc
      601 gcgccggcca catagctcaa ctacctggtg atcgataggc agcaacgacg actcgatcaa
      661 agcgccggcg ccactcgcag cacacagatg attaaggtca gacgaaagcc cgtcgctgat
      721 atcgatcatc gacgtcgcaa gacgttcttc accaagcacg attccccagc cgacgcgagg
      781 ctccggtcgg agctggcgca acaacacatg gtcgagcttt tgcgaatcgt cgtctgcaag
      841 gttctgttcc gcgaggtgtg cgccgcgttc gatcagacgc aaaccggcgg cggcgccgcc
      901 caatgaacca gtgacaaaga tctgatcgcc tggttgcgcg ccggcacgct tcacggcgtg
      961 gccggcactg cattcaccaa gcacgatcga atcgacgact atcttctcgt gtgtgcgtga
     1021 agtgtctcca ccgatcaact gcacgtcgta acgttgtgcg agactggcga gtccttcgta
     1081 aaactgatcg acaaactcag tttgccaaac atcctcgggt acgccaatcg agattaacga
     1141 ccaacgcggg cgcgcgccca tcgctgcgat gtctgaaagc gaaactgcga gtgctttgtg
     1201 tccgagaagt agcggtggtg cagtagtacg gcgaaagtcg atgtcttccg cgagtaagtc
     1261 agtcgtgata acgatttctt tgttcggtgt gttacgcagg accgccgcgt catctccaat
     1321 ccccgcgccg aatctgcggc gcagggagtc gatgaattcg aattcgcttc ggctcatcgt
     1381 tagaggttac gacgaagcga atcaagaccg gcttctatag tttcaattga ggtagagaac
     1441 gagaaacgca gatagccctc gccatcgacg ccgaagacaa ttcccggaac cgtcgcaacc
     1501 tgcgcgcggt tcagcaggat gtcggccgct tcacggctat cgggatggat cagtcgcgca
     1561 tcgggaaaag cgtagaatgc gccggccggc cgcgcgcagg tgagtccgat ctcattcaaa
     1621 cctgacagga gcagatcgcg tcgcacgcga tactcgtcgc gcgctcgatc gagcagcgcc
     1681 ggatccatat tcagcgccgc gactcccgcc cattgcgttg gcgtcgagac accgttggag
     1741 gagtacaacg tggctttttt caaacccgtc atccatggtt cggttgcaac tgcgtaaccg
     1801 agacgccaac cggtcatcgc atacgacttg gaaaacgtga agcaggagat cgttcgttca
     1861 aacattccgt caagcgacgc gatcgaacaa tgctcgccgt cgtagaccag atcttcgtag
     1921 gcttcgtcgg caattactac gaggtcgcgt tcctgcacga aagaagccac ggcgttagct
     1981 tcattaggcg tgagcacgat gccgttcgga ttctgcggtg tgttgtagta gatcgctttc
     2041 gttcgcgcgg tagcgtaacg ggcgagcgtt tgctggaatc ctgcttgacg cgcttcgtca
     2101 gttggcacga gtacgatgcg gccctggcaa tgcgcaacga gatctttgat cggcgtccag
     2161 taaggagaga agagcaacac ttcgtcgccc ggattgacga cagattgaaa cgcgccgaac
     2221 agtgcctgca tgccgccatt cacgacgata acgttttttg cttccgcagg aatgcggttt
     2281 cgcgtgcgaa gtttctcggc gatagcctcg cgcagttcgg gaattccgga ggaaggagcg
     2341 tagcgtgttt tgttttccgc gagcgctcga gtcatggcct ctttgatcgg ttcgggcgtc
     2401 ggcatgaacg gttcaccgcc ttcaaactgg taaacagttg cgccttgctc gcgcagctcc
     2461 atgatgcggt tgcgcagttt gacgatgtca gagaatccga cctccgtgag ccgcgaagcg
     2521 agccgcagaa ccgttggcga ggttgtcact gtttcggaat cgttccttcc agatgtcggt
     2581 ggttgggacg aaggattata gcttgcgcgg cgagtgagtc caaatcgttg tgtttgttct
     2641 ttgaacttgg tccttggttc tttgttggaa accaatctct ccgcagctga tgcgaactta
     2701 tcgaccgcta acaaagaaca aagtacagag atccaagttc agaagatact tttaaccaaa
     2761 actttgaagt gagccgcgct aagtgtaagt attgacacgc caaacttcac gcgagtaaca
     2821 tgtcgttcga cgcacggctg ctggcaaatt gccgtagact tccactttcg caacgcccga
     2881 tataaagagc cagctcagca actcaaattg aggctttaac tgcatgagtc gtcctgctgt
     2941 agctataccc gaaaaacttt ttttcaagat tggtgaggtg tgtgagcttg ccggagtgca
     3001 ggcgcacgtg ctgcgttatt gggagtcaga gtttcctatg ttggcgccgc agaagaaccg
     3061 cgccggacag cgcgtgtatc gcaagcgcga cgtggaaatg gcgctgcgga tcaaggagct
     3121 gctttacgag gatcagtaca cgatcgcagg cgcgaagaag cggctcacga atgagttgcg
     3181 tggcggcggc aagttgaaag ttgttggcga ggaagacggg caggcgccga acggatctgc
     3241 cgagagcgaa agctttacgc ctgctaacag ttcttatgcg tcgagtgtag cgccgttggc
     3301 agcggaagag cggcagggat tgaagcaact cgccgcggag ttgcgcgaat tactttcgct
     3361 gctcgagcag gaaaccaagg gacaggcaaa gtaggccacg gatattcgcg gagagacacg
     3421 gatcgggctc cgcagtttga cagtcttctt tgtttgacaa ttcttgtttg gcaggatagt
     3481 aactcagtga aacgggacgt ggcgcagcct ggtagcgcgc acgcttgggg tgcgtgaggt
     3541 cgctggttcg aatccagtcg tcccgaccat ttctaacaac ccctacggct gccggcctcg
     3601 gcagccgttc tgcgttttgg cagacaccag atctgaatat gcgtaaactg tctcgctgga
     3661 tgccgcaaac tcccacgaaa cgcgtgatcc caatgagcat cttcctgctc gcggtgttag
     3721 cggtggtgct catctgcctc gctttatcaa ctaccgcgtt cgttttggtg accggtttta
     3781 ttctcctcgt tactctcatg agcgtgtacg accgtctccg cctgcggcgt ctcgctgagc
     3841 agcgtatggg ggagagcatt tgtacatttg tccgctcttt cgaccgccat gtggttgatc
     3901 cgtgggttat cagggctatg tacgaggagt tccacgcgta tttcgatggc gcgctaccaa
     3961 tccgcgcggc tgatcgcatc gaggaagatt tgcgcatgga ttgggacgac gtggatgatc
     4021 tgctcagcga cgtcgcgttg cgcgcggggc gctcattgga tcgcatagaa tcgaatccgt
     4081 tgtatggtca gttgcgaact gtagcggatc tggtcctgtt cttaatgcac cagccaaagt
     4141 gcggtgcagc ataacgcgtt acagcgccag ccggagccga agatccaccg agatctggga
     4201 cagtaaactc caagtaaatt ctcgtgcgaa tccgttttca accggacgcc gaagccacca
     4261 aaaagtgaga tcccgtttgc ccgaagagcg ctgataacag ctaattcaga accagccttc
     4321 actcggttcg ctgtcgtgcg acgtctcgtc gtctttcagc tcagtaacaa cgatcgccga
     4381 agtaagtgtc ttgtgaaccg ggcacttgtg tgcgatctcc tgaagtcgcg cgtgctgctc
     4441 ttcagtcagt ggaccagtta ctgtgaccgc acgctcgata cgatgaatgt atccttcggt
     4501 ctgctgacag tccttgcagt cttttgcgtg gattcgattt tgccttaaac gaacactcac
     4561 tgattcgagc ggccactgct ttctgcgcgc gtagagcgtt actgtcatcg agatacaact
     4621 gccgagtgcg gcgagtaaca gcgtatacgg atccggccct gcgtcttcgc cacccgcagc
     4681 aacgggttca tccgtcgtaa aagaatggcc cgcgccgtag cgcacctcat ttcgcaaatt
     4741 accttgagac ttaacaatca cttcgctcat ctcagatgca cctccatagc tggactaccc
     4801 ggtctaagtt cgccgctgcc attcagctgg gtctctggca cagtgaagca ctgcaaggat
     4861 cattacggtg tcgtcttcga cactgaaata
//
LOCUS       DASTEY010000391         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1574252, whole genome shotgun sequence.
ACCESSION   DASTEY010000391 DASTEY010000000
VERSION     DASTEY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574252"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1147)
                     /locus_tag="VFO72_06855"
     CDS             complement(377..1147)
                     /locus_tag="VFO72_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9479042.1"
                     /translation="MSILIAEDNMAQRHYLREILEKEFPAHVPVFEAGDGEETVKIAL
                     REKPEVCILDIQMPKLSGVKAARAIWRDFPTARIIFWTQFPHEIYINEIRKIVKSVDP
                     PPAYGFIHKNNPESRFLRFVAAVLEDGADMIDPAFKDSFKRPLLTEFEAEALYYLALG
                     LSNWAIARKCSLSLRGVESRLATLYEKLFVSVAEGTDQEAYEKLAYNIRTRAFFEAVR
                     RGLLNSDELEKASNDLEAWLQKDQKRFNEEQKLASKKK"
     gene            1195..>2325
                     /locus_tag="VFO72_06860"
     CDS             1195..>2325
                     /locus_tag="VFO72_06860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9479043.1"
                     /translation="MRPRKLSVMVLLSLSVVSTVVPTVAQTRDERLWQRALQIHRRAV
                     VVDTHNDVTTPMTNDDFDLSGTPPTPYRTNIERMKKGGLTAEFFSLYIKPWYVEHGGS
                     ARRTLDMIDSVYRAVERHPRDLMFATSVADIRRAKRQGKIAALMGIEGGHAIEDSLPT
                     LREFYRLGVRYMTLTWNNTNNWADAGRGEKKHNGLSDFGKEVVREMNRLGMLIDVSHV
                     SDKTMSDALDVSKAPIIASHSSARAISNVSRNIPDDLLKRIAKNGGVVHVNFYSVFVD
                     TQTVSPQSAERDRRLKAQQDAINERYKNDPERRAEESDKLEAANPLPPLPISKLIDHI
                     DHIVKVAGIDHVGIGADFDGANDMPEGAQDVSMLPNITYELLK"
BASE COUNT          524 a          636 c          660 g          505 t
ORIGIN      
        1 gccagtatgc gccgccctac gggcactgcc ggatcgctga ttgtttccgt tcgtcggcca
       61 taaaatctcg cgagacctgt ttcagtgcca aaccacatgg ctccatcggg cgcttgcgta
      121 atagcgcgca cgcgatcgga aggcaggcca tgaaacaacg taaccgcacc ccattggtgc
      181 agacctgtaa cgggcgttgg cgaaggtgat ggagacggtg aaggcgaagg cgacggcgat
      241 gcctgcggct gcggttcgct ttcctgtgct gagccactaa gaaaaggcaa aaccaaaacg
      301 gtcagccaca aaaaggcaca aaaagcacaa atgatctttc ttatgcattt tgtgcttttt
      361 gtgccttttg gtggcctcat ttcttcttcg aggcgagttt ctgttcttcg ttgaaacgtt
      421 tctggtcctt ttgtagccac gcttcgaggt cgttcgaggc cttctccagt tcgtcgctat
      481 tcaacaaccc gcggcgcact gcttcgaaga aggcgcgcgt gcgaatattg taggcgagct
      541 tttcataggc ctcctgatcg gtcccttccg cgaccgacac gaagagcttt tcgtaaagtg
      601 ttgccagccg gctctcgaca ccgcgcaagc tcaacgaaca cttgcgcgcg atggcccagt
      661 ttgaaagtcc gagtgccagg tagtaaagcg cctctgcttc aaactccgtc aacaacggtc
      721 gcttgaacga gtccttgaac gcgggatcga tcatgtccgc gccgtcttca agcactgcgg
      781 caacgaaacg aagaaacctc gactccggat tgttcttgtg aatgaagcca taagccggcg
      841 gtggatcgac agacttcacg atcttgcgaa tctcgttgat gtagatctcg tgtggaaact
      901 gtgtccagaa gatgatgcgc gcggtaggga agtcgcgcca gatggcccgc gcggctttca
      961 cacccgacag cttgggcatc tggatgtcga gtatgcagac ctcaggcttc tctcggagcg
     1021 cgatcttcac ggtctcttcg ccgtcgcccg cctcgaatac cggaacgtgc gcagggaatt
     1081 ctttttcgag aatctcgcgg aggtaatggc gctgggccat gttatcttca gcaatcagga
     1141 ttgacatgtc gattgatgat aacgcttcat gtgaccacga cgaaaggtga actcatgcga
     1201 ccccgaaaac tttctgtaat ggttttactc tccctgtctg tagtgtcaac cgtagttccc
     1261 acagttgcgc aaacgaggga tgaacggctc tggcagcgcg cgctccaaat ccatcgccgg
     1321 gcggtcgtcg tcgatacgca taacgacgtc acaacaccga tgacaaacga cgatttcgat
     1381 ctgagtggca cgccaccaac gccgtaccgc accaacatcg agcgtatgaa aaagggtggg
     1441 ctcacggcgg agttcttttc gctttacatc aagccgtggt acgtcgaaca cggcggctcg
     1501 gcgcgtcgca ccttggacat gatcgactcc gtttatcgcg ctgtcgagcg gcatccgcgt
     1561 gatctaatgt tcgcgacttc tgttgccgac attcgccgcg ccaagcgaca gggaaagatc
     1621 gcggcgttga tgggcatcga aggcggtcat gcgattgaag actcgttgcc aacgttgcgt
     1681 gagttttacc ggctcggcgt gcgatacatg acgctcacct ggaacaacac caacaactgg
     1741 gctgacgcgg gtcgcggcga gaagaagcac aacggattga gcgacttcgg caaagaagtc
     1801 gtgcgcgaga tgaatcgttt ggggatgctg attgacgttt cgcacgtgtc tgacaagacg
     1861 atgagcgatg cgctcgacgt ttctaaagcg ccgatcattg cctcgcactc gtcagcgcgc
     1921 gcgatcagca acgtctcgcg aaatatcccg gacgatctgc tgaaacggat cgcgaagaac
     1981 ggtggcgtgg tccatgtgaa cttctacagc gtttttgttg acacgcagac agtctcgccg
     2041 caaagcgcgg aacgcgaccg caggctcaag gcccagcagg acgccattaa cgagagatac
     2101 aaaaacgatc cggagcgccg tgcggaagag agtgacaagc tcgaagcggc gaatccgttg
     2161 ccaccgcttc cgatctcgaa gttgatcgat cacatcgatc acatcgtgaa agtggcaggt
     2221 atcgatcatg tcggtatcgg cgcggatttc gatggtgcga acgacatgcc tgaaggtgcg
     2281 caggatgttt cgatgttgcc gaacatcact tacgagctgc tgaag
//
LOCUS       DASTEY010000392         7140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_24996, whole genome shotgun sequence.
ACCESSION   DASTEY010000392 DASTEY010000000
VERSION     DASTEY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24996"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFO72_06865"
     CDS             complement(<1..316)
                     /locus_tag="VFO72_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479044.1"
                     /translation="MFFLQANTTSLINVADLQLIAPELILTACACVALIMEVILPYRK
                     SKLTAYFALVGVALAFVSLGFQWWTMRGALPFDGFYGMVRIDGFALLFKAIFLVAAAL
                     AIG"
     gene            complement(326..2026)
                     /locus_tag="VFO72_06870"
     CDS             complement(326..2026)
                     /locus_tag="VFO72_06870"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9479045.1"
                     /translation="MSNLHILSIITWLPALGAILILAVMKKDQGNLIKKFATAWFVLD
                     FIASLWLLKYNRGVGGMQFLEDHQWIPVIGARYQMGVDGVAVLLIILTTLLGAIAALS
                     SWDYIKKREKEYYVLLLLLQTAVIGVFASMDLFLFYLFFEVSLVPMYFLIGVWGGENR
                     LYAAIKFFLYTLVGSVVMLLGVLKMYFLTQDTALAGQISQATVNLLAGNSQAAQMVAT
                     SLQAAAANGTGTFNIIYMQAMGSVLPMGTMQILLFIAFALGFAIKVPMFPFHTWLPDA
                     HVEAPTAGSVILAGILLKLGTYGFYRFNLPMFPKASMDQSSWVTSFGTFGVRSVVVFL
                     AIVSIIYGALAAMYFVVKKDGDVKKLVAYSSVSSMGMVMLGLFAVNPNGVNGGVMQMI
                     NHGIYSGALFLLVGIIYERRHTRNVAEFGGLSHVMPGYAAIFLGMVMTAIGLPLLAVF
                     ISEFLALRGAFEANVYWAGWAALGIILNAGYFLWLYQRMFFGTIDNPKNERLPDLNVR
                     EWAYMVPLMILSLWIGVYPKPFLEYIQQPVNALVKQVRPEYQIPGMPQQAPETERAQR
                     "
     gene            complement(2075..4489)
                     /gene="nuoL"
                     /locus_tag="VFO72_06875"
     CDS             complement(2075..4489)
                     /gene="nuoL"
                     /locus_tag="VFO72_06875"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9479046.1"
                     /translation="MYKYIWLVPLLPLIGAAINGFFGRKFRFSEPLIGGIAVGSVALA
                     FLISVAAIYSYGFGGHPQWPNPYLTSQDGFSFNWIPGGAVSINQGSDERRQAQLEKQI
                     AEREASMTPEERAHPEPQIYATVESPTYALLNIEWSYQLDPLSAVFMLIVTGVGLCIF
                     VFATGYMHGDAGFYRFFAYMGLFMFAMLVLVMGSNFLMMFVGWEGVGLCSYLLIGYYF
                     DRQEAADASRKAFITNRVGDFGFALAIFGIIATFGSAQYTEVFNQAKAYPIELLGHWG
                     VLSWIALGLFIGACGKSAQLPLHVWLPDAMAGPTPVSALIHAATMVTAGLYMVTRTNV
                     IFQHSQTMLLVVALIGMATAIFAAIIGITQNDIKKVLAYSTVSQLGFMFMAAGVGAFA
                     ISIFHVMTHAFFKALMFLGAGSVIHGMHHEQDMRRMGGLKKYMPYTYWTFVAGWLAIC
                     GIIPFSGFWSKDEILWNAASTTAIPLGWLVWLVGTIAATCTAFYMTRLMAMTFWGKER
                     FLEKPETHDIHGDHGHAAHVPHESPRAMWVPLVVLAVLATIGGLIGIGPAFKPITGTD
                     HPGDRLNIVNWLKPIVWDPVTREFPTGAAHGPVAEHAAPAPVPEASHGETHWFNLAHA
                     VDNVLHNHLFTEWTFIIISLVVAALGIGLGMLFYVKSHALPDIWARRLRPLYNASYNK
                     FWVDEFYGWAVTRRVMDSARGVFAFDSRVIDGIVNGLATLSRGVSLVVGGIDKYFVDG
                     IVNTIAAFIKRLMSPLFRAAQTGFTQNYALVMVVGLMIAVVVFFASDVARAITSAVRR
                     IFATVM"
     gene            complement(4570..4974)
                     /gene="nuoK"
                     /locus_tag="VFO72_06880"
     CDS             complement(4570..4974)
                     /gene="nuoK"
                     /locus_tag="VFO72_06880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9479047.1"
                     /translation="MLNAFIPTLVFLFQAEGGWLAQVKQGAANPDLAKFLVIGALLFI
                     IGVAGVLTRRNIIVIFMSIELILNAANLNFIAFARYLQDSGNMNAVAGQIFAVFVIVV
                     AAAEAAIGLGIVIALYRNKETIWVDEIDLLKW"
     gene            complement(4976..5653)
                     /locus_tag="VFO72_06885"
     CDS             complement(4976..5653)
                     /locus_tag="VFO72_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9479048.1"
                     /translation="MTLSGYEALFFWIFALAAIFAGLLTVMARNAVHSALFLISSLVS
                     VAALFVLAGAEFIAGVQILVYVGGVMVLFLFVIMLVNVGAEEKGREEIFNKERQVTAS
                     LIFCFLLVIGVLYGVTEGHRGLQARPQPETSASQAARERSLPTGATGTSRITEDTERV
                     GSSLYRYASLPFEIASVLLLVAIIGSVMLARTLRQEASADDVDPETLQEEIALDPELA
                     KLEPRSE"
     gene            complement(5674..6207)
                     /locus_tag="VFO72_06890"
     CDS             complement(5674..6207)
                     /locus_tag="VFO72_06890"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET9479049.1"
                     /translation="MPLVKTPKPTFAQKLKRFFMVDLIKGMGLTLKYNLGAIIDKDAV
                     AGKGIYTEQYPKVRPDVAPRFHGAPRLNMDPETHDTLCIACNLCAIACPEDCIDVIAM
                     DVEIVVAGKPRRKKVLDEFIFDTSRCMFCALCQEACPTSCLELTQDFELATYSRAGFV
                     WNREMLEQGRETVKYTS"
     gene            complement(6256..>7140)
                     /locus_tag="VFO72_06895"
     CDS             complement(6256..>7140)
                     /locus_tag="VFO72_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET9479050.1"
                     /translation="VSSVGVLALILAGWSSNSKYALLGGLRSSAQMVSYEVAMGLSLI
                     GALMFARTLSLSGMVAAQGSDSMWYILYQPVGFMLFLISGIAENNRAPFDLPEAESEL
                     VAGFHTEDSGMRWSLFFMAEYAAMVVVAAVATTVYLGGWYFPFVYRLETSGHHNLYVL
                     VSVLVFLVKASIILYIYFWLRWTLPRFRYDQLMDIGWKWLIPSALINIVLTAFAVFLL
                     QALNGWRGIQTIESLDRGMNLTATGKGIMIAIGVAGLFITGGLLARINWRSRDFNLKS
                     QRRNIRLVNVPKGKPARA"
BASE COUNT         1725 a         2114 c         1928 g         1373 t
ORIGIN      
        1 tacctatggc cagtgcggcg gcgaccagaa agatcgcttt gaataacaga gcaaacccgt
       61 cgattcgcac catgccataa aaaccgtcga acgggagcgc gccgcgcatg gtccaccact
      121 ggaaaccgag tgagacaaag gccagggcca cgccgacgag cgcgaagtaa gcggtgagtt
      181 tgctcttgcg atacggaagt atgacttcca tgatcagcgc cacgcaggcg caagcggtca
      241 ggatcaactc gggcgcgatc agttgcagat ccgcgacatt aatcagtgac gtcgtgtttg
      301 cttgaaggaa gaacattggt tctttttatc gttgcgcgcg ttccgtttcc ggcgcctgct
      361 gtggcattcc cggaatctga tactccggcc gcacctgttt caccaacgca ttgacgggct
      421 gctgaatgta ttcaagaaac ggtttgggat agacgccgat ccacagcgag aggatcatca
      481 gcggcaccat gtacgcccac tcgcgaacgt tcaggtccgg cagccgttcg ttcttcgggt
      541 tgtcaatcgt gccgaaaaac attcgctggt agagccagag aaagtagccg gcgttaagaa
      601 tgattccgag tgccgcccag cccgcccaat agacgttcgc ctcgaacgcg ccgcgcagtg
      661 cgagaaattc cgaaatgaaa acggcgagca gcggcagtcc gatcgcggtc atcaccatgc
      721 cgagaaagat cgcagcgtag cccggcatga cgtgcgacag tccgccaaac tccgccacgt
      781 tgcgcgtgtg gcggcgctcg taaatgatgc cgacgagcag gaacagtgcc cccgagtaga
      841 tcccgtgatt gatcatctgc ataacgccgc cgttgacgcc gttcggattg accgcaaaca
      901 gtccgagcat caccatgccc atcgacgaga cggacgagta agcgaccaac ttcttaacgt
      961 cgccgtcttt cttgaccacg aagtacatcg cggcgagtgc gccgtagatg atgctgacga
     1021 tcgccagaaa caccacgacg ctgcgcacgc caaaggttcc gaagctcgtc acccaactcg
     1081 attggtccat cgaggctttg ggaaacatcg gcaggttgaa acgatagaag ccataagttc
     1141 cgagcttgag cagaatgccc gcgagaatca ccgaacccgc agtcggagct tcgacgtgtg
     1201 catccggcag ccaggtgtga aacgggaaca tcggcacttt gatcgcaaag ccgagggcga
     1261 aagcgatgaa cagcaggatc tgcatggtcc ccatcggcag caccgatccc atcgcctgca
     1321 tgtagataat gttgaatgtg ccggtgccat tggcggccgc tgcttgcaaa ctcgtcgcga
     1381 ccatctgcgc cgcttgactg ttcccagcga gcagattaac tgtcgcttgc gaaatctgac
     1441 ccgctaacgc tgtgtcttgc gtcaggaagt acatcttcaa gacgcccagc agcatcacca
     1501 ccgatccaac cagcgtgtac aggaaaaact tgattgccgc gtacagcctg ttctcgccac
     1561 cccaaacacc gatcaggaag tacatcggca cgagcgagac ttcgaaaaac aggtagaaca
     1621 gaaacaggtc catcgacgca aacacgccga tcactgccgt ctgcaacaac agcagcagca
     1681 cgtaatactc tttctcgcgc ttcttaatgt agtcccatga cgacagcgcc gcgatagcgc
     1741 cgagcagcgt cgtcaggatg atcagcagca cggccacacc gtcgacgccc atctggtaac
     1801 gcgcgccgat gaccggtatc cactgatgat cttcgagaaa ctgcatgccg ccgacgccgc
     1861 ggttgtattt caacaaccag agcgacgcga tgaagtcgag aacgaaccag gccgtggcaa
     1921 acttcttgat caggttaccc tgatcttttt tcatgaccgc cagtatgagg atggcgccca
     1981 gggccggtag ccaggtgatg atcgaaagaa tatgcaggtt actcatttga atatggtgga
     2041 actttcagtc ttggaatccc gtcaacttct atcgctacat cacggttgca aatattcgac
     2101 gcaccgcgct tgtaatcgcc cgtgcgacat cgctcgcaaa gaaaacgacg accgcgatca
     2161 tcaaccccac aaccatcacg agcgcgtagt tttgcgtgaa tcctgtctgc gccgcacgga
     2221 acaaaggcga catcaagcgc ttgatgaatg ccgcgatcgt gttgacgatg ccatcgacga
     2281 aatacttatc gattccgccg acgaccagac taacgccgcg gctcaacgtc gccagcccgt
     2341 tgacgattcc atcgattacg cgcgagtcga acgcgaaaac acctcgcgcc gaatccatca
     2401 cacggcgggt gacggcccat ccgtaaaatt cgtcaaccca gaatttgttg tagctcgcgt
     2461 tgtaaagcgg ccggagtcgt cgcgcccaaa tgtccgggag cgcatgactc ttaacgtaga
     2521 acaacatacc taacccgata ccgagcgcgg ccacgacgag cgagatgatg atgaacgtcc
     2581 actcggtgaa cagatgattg tggagcacgt tatcgacggc gtgggccagg ttaaaccaat
     2641 gtgtctctcc gtgtgacgct tccggcacgg gcgccggcgc cgcatgttcc gcaacaggtc
     2701 cgtgggccgc accggtcgga aactcgcgcg ttaccggatc ccagacaata ggtttcagcc
     2761 agttaacaat attgagtcgg tcaccggggt gatcagttcc tgtaattggt ttgaatgccg
     2821 gtccaattcc gatgagacca ccaatcgtcg ccagcacggc caacacaacc agcggcaccc
     2881 acatcgctcg cggtgattcg tgtggtacat gcgcggcatg tccatggtca ccgtgaatgt
     2941 cgtgagtctc cggtttttcg aggaaacgtt ccttgcccca gaacgtcatc gccatcaagc
     3001 gcgtcatgta aaacgccgta cacgtcgccg caatcgttcc gaccagccac acgagccaac
     3061 cgagcgggat cgccgtcgtc gatgccgcgt tccagagaat ctcgtccttg gaccagaagc
     3121 cgctgaacgg aatgatgccg cagatcgcca accatccggc cacgaacgtc caatacgtat
     3181 agggcatgta cttcttcaat ccgcccatgc ggcgcatgtc ctgttcgtgg tgcatgccgt
     3241 gaatgacgct gcccgcgccg aggaacatca gcgctttgaa gaaggcgtgc gtcataacgt
     3301 gaaagatgga gatagcgaaa gcgccgacgc cggccgccat gaacatgaag ccgagctgcg
     3361 aaaccgtcga gtacgccagc accttcttga tgtcgttctg cgtgataccg atgattgcgg
     3421 caaagatcgc ggtggccatg ccgatgagcg cgacgaccag cagcattgtt tgcgagtgct
     3481 ggaaaatgac gttggtgcgc gtcaccatat aaagaccagc ggtaaccatc gtggcggcgt
     3541 gaatcaatgc cgagacgggc gttggtccgg ccatggcgtc gggcaaccac acgtgcaacg
     3601 ggagttgcgc tgacttacca cacgcgccga tgaagagtcc aagcgcgatc catgagagga
     3661 cgccccagtg accgagcaac tcgattggat atgctttggc ctgattaaag acttcggtgt
     3721 actgcgcgct gccaaacgtg gcgatgattc cgaagattgc gagggcgaaa ccgaaatcgc
     3781 cgacccggtt cgtgataaac gccttgcgag aagcgtccgc cgcttcctgt cgatcgaagt
     3841 agtagccgat gagcaggtag gaacagagac ccacgccttc ccaacccaca aacatcatca
     3901 ggaagtttga gcccattacg agcacaagca tcgcgaacat gaacaggccc atgtaagcga
     3961 agaaccggta gaagccggca tcgccgtgca tgtagccggt cgcgaagaca aagatgcaca
     4021 acccgacgcc cgttacgatc agcatgaaga ccgccgagag cggatcgagt tggtaactcc
     4081 actcgatgtt gagcaacgcg tacgtcggcg attcaactgt cgcgtagatc tgcggttcag
     4141 gatgggcccg ttcttcgggc gtcatcgacg cctctcgttc cgcgatctgt ttttcgagtt
     4201 gagcctggcg ccgttcgtcg gaaccctggt tgatgctgac cgcgccgccg ggaatccagt
     4261 tgaaactaaa accgtcctgg ctggtcaggt agggattggg ccactgcgga tgaccaccaa
     4321 agccatacga atagatggcc gcgacgctga tgaggaaagc gagtgcgacg gagccgacag
     4381 cgataccgcc gatcaacggt tcggaaaagc gaaacttccg tccgaaaaag ccgttaatag
     4441 ccgcgccgat cagtggaagc aggggaacta accaaatgta tttgtacatt cgttttgtgc
     4501 ttggtgcttt gtacctggta ctttgcagta aagacaaagc acgaagcaca aggtactaag
     4561 ctttcttggt taccacttca acaaatcaat ctcgtcaacc cagatcgttt ccttgttccg
     4621 gtaaagcgcg atcacgatgc ccagaccgat cgccgcctcg gccgccgcta cgacgatcac
     4681 gaacaccgca aaaatctgcc ccgccacggc attcatgttg ccgctgtcct gcaggtagcg
     4741 agcgaaggcg atgaaattga ggtttgcggc gttgagtatc aactcgatcg acatgaagat
     4801 gacgatgatg tttcgcctgg tcagaactcc cgccacgccg atgatgaaga gcagagcgcc
     4861 gatgacgaga aacttcgcca ggtcgggatt cgcggccccc tgctttacct gggccagcca
     4921 gccgccttcg gcctgaaaca agaacacgag tgttgggatg aaagcgttga gcatgttatt
     4981 ccgaccgagg ctccagcttc gcgagttcgg gatcgagtgc tatttcttcc tgcaaagtct
     5041 cgggatcgac atcatcggca gacgcttcct gtcttagagt ccgcgccaac atcaccgatc
     5101 cgatgatggc taccagcagc aacacactcg cgatttcaaa cggcaacgag gcgtatcgat
     5161 agagactgct gccgacgcgt tcggtgtcct cagtgatgcg gcttgtgccc gttgctccgg
     5221 taggaagcga tcgttctctc gccgcctgcg aggcggacgt ttccggctgg ggccgcgcct
     5281 gcaaaccccg atgaccttca gtcacgccat acagcactcc gataaccaac agaaaacaga
     5341 agataagact cgccgtgacc tgtctctctt tgttgaagat ctcttcgcgg cccttctctt
     5401 ccgcgccgac gtttacaagc atgattacga agaggaatag gaccatcacg ccgccgacgt
     5461 agaccagtat ctgcacaccg gcgatgaact ccgcgcccgc gagcacgaag agcgccgcca
     5521 ctgagaccag cgacgaaatg agaaatagcg cgctgtggac tgcattgcgc gccatcacgg
     5581 tcaacaaccc cgcgaagatc gcggccagcg cgaagatcca gaagaagagg gcttcgtagc
     5641 cggagagagt cattattaag attcgtgttg gtgttagctc gtgtacttaa cagtttcgcg
     5701 gccttgttcg agcatctcac ggttccagac aaacccggcc cgcgaatagg ttgctaactc
     5761 gaaatcctgc gtcaactcga gacacgacgt cggacaggcc tcctggcaaa gcgcgcaaaa
     5821 catgcatcgc gacgtgtcga agataaattc gtcgagcacc ttcttgcgcc gcggtttgcc
     5881 ggcaaccacg atctcgacgt ccatcgcgat cacgtcgatg cagtcttcag gacaggcgat
     5941 cgcgcacaga ttgcacgcga tacagagcgt gtcgtgcgtc tcgggatcca tgttcaggcg
     6001 cggtgcgcca tgaaaccgtg gagcgacgtc gggacgtact ttcggatact gttcggtata
     6061 gattcccttg cctgcgaccg cgtccttatc gatgatcgcg ccgaggttgt acttcagcgt
     6121 cagccccatc cccttgatca gatcaaccat gaagaagcgt ttgagtttct gcgcaaatgt
     6181 tggtttcggg gtcttaacta acggcatttg ttacctcatc aatccacacg gatttcacgc
     6241 ccgtgctggc ccgccttacg cccgcgccgg cttgcctttc ggtacattca ccaacctgat
     6301 gttgcggcgc tgcgacttca ggttgaaatc gcgcgagcgc cagttgattc gcgccagcag
     6361 cccgccggtg atgaatagac cagcgacgcc gatcgcgatc atgatgcctt ttccggtcgc
     6421 ggtcagattc attccacgat cgagcgactc gatcgtctgt atgcctcgcc aaccatttaa
     6481 agcctgcagc agaaataccg caaacgcagt cagcacgata ttgattagcg ccgaaggaat
     6541 cagccacttc cagccaatgt ccatcaactg atcgtaacgg aaacgcggca acgtccagcg
     6601 caaccagaag tagatgtaaa gaatgatcga cgccttaact aaaaagacca gcacgctcac
     6661 cagcacgtaa agattgtgat gcccactcgt ctcgagccgg taaacgaacg gaaaatacca
     6721 gccaccgaga tagaccgtcg tcgccaccgc cgccacgaca accatcgccg cgtattcggc
     6781 catgaagaac aacgaccagc gcatgccgga gtcttcagta tgaaaaccgg cgaccagttc
     6841 cgactccgcc tccggtagat cgaacggcgc gcgattgttc tcggcaatac cgctgatcag
     6901 gaacagcata aaaccgaccg gctgatacag gatgtaccac atcgagtccg aaccttgcgc
     6961 cgctaccatt cccgaaagcg acaatgtgcg cgcaaacatc agcgcaccaa ttaacgagag
     7021 ccccatcgca acttcgtacg acaccatctg cgcgctcgat cgcagtccac ccaacaaggc
     7081 gtacttcgag tttgacgacc agcccgcgag aatcaatgcc agcacaccca cgctcgatac
//
LOCUS       DASTEY010000393         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1393028, whole genome shotgun sequence.
ACCESSION   DASTEY010000393 DASTEY010000000
VERSION     DASTEY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393028"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFO72_06900"
     CDS             <1..326
                     /locus_tag="VFO72_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9479051.1"
                     /translation="GIYMKINYVNADHTHALIDLPTNICIEDVIKLFKGSSSHWINQN
                     RVIRSKFFWGRGYGAFSVSHSDVDRVARYIANQEEHHRKHTFAEEYELFVKRYGLEWR
                     DEGNR"
     gene            complement(799..1968)
                     /gene="nagA"
                     /locus_tag="VFO72_06905"
     CDS             complement(799..1968)
                     /gene="nagA"
                     /locus_tag="VFO72_06905"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET9479052.1"
                     /translation="MNTNMAQTPVVLKNARVVLPDRVMESGTVVVDAGLITSVADSAT
                     AEGEVLDLSGLILLPGFIDVHTHGAVGIDVMEATASELQVVSEYLASQGVTAWLPTLV
                     PAADADYGNVAAAVSQAMGTPDGVGARILGLHYEGPFVNSAQCGALHVEHFKTYSGAS
                     DLDRLPVSEETVRMITLAPEIAGGVELVRELKRRGWVISIGHTRATVEVLDQALAAGA
                     HHMTHFMNAMSPLHHRSPGPIGWGLSHDNVTFDMIADGIHLDPLMLKLLVKIKGARGV
                     SLISDAIAATGKGDGDYEIWGETISVKNGRTSNAAGSIAGSVITMLDGVRMMRSLGIC
                     FPDLAQLASSNPARLLGLSNARGSIETGKRADLVALDTNGNVRLTIIAGQVAFSA"
     gene            1998..3146
                     /locus_tag="VFO72_06910"
     CDS             1998..3146
                     /locus_tag="VFO72_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479053.1"
                     /translation="MIRFTPSLLPLLLICATAPAQPPRHQAERVRTYYETARKFEAAG
                     NWRQAEQTWRAVLKLAGNDARAWTNLGVALNRQDKTAEALEAWKHAISIDPKLPGPYF
                     NLGLTFVRQGDYDNAIAPLRQALSLAPNNDGARRALAIALMAQEKFQEASREIAQLLF
                     RNPEDAALLELAAKSFMSQRRYQEAAAVLQRRIRLPNMTVFLWSQYGDALDGAGLTPE
                     ALEAYRKAVELDPESTLTRYGLGYLYWKLYRYDDAERELLEVLRRAPNDPRASFTLGD
                     LYLTRGDAQRALPLLETARAAYPNEFDTRFALGRALILTGNLDRGIDELRAAVALDDG
                     IADGHFQLGRALIKAGRNEEGKLELQKAQTLHDKQRKNEAERLRKKLP"
     gene            complement(3246..>3801)
                     /locus_tag="VFO72_06915"
     CDS             complement(3246..>3801)
                     /locus_tag="VFO72_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479054.1"
                     /translation="TSRPHIFSASYVYELPFFRKHPSAWARGFLGGWQIAGITDISSG
                     QPVARVVSGASNIAATIGQYPNVVSDPNAGLAGTIDPVSGLPYIFDPRAFTAPLAGTY
                     GNAPRAFARLFGRNQTNLTLTKNIYFDNEDRFKIQLRAEAFNVFNHTQFTGSGTTLTT
                     TSTFGLPLGARLPREFQFGAKLLF"
BASE COUNT          888 a         1105 c         1031 g          777 t
ORIGIN      
        1 aaggaatcta tatgaagatc aactatgtta atgccgatca cactcatgcg ttgattgatc
       61 ttcctactaa tatttgtatt gaggacgtca ttaagctgtt caaagggagc tcgtcacact
      121 ggatcaacca gaaccgagtt attagaagta agttcttctg ggggcggggc tatggagctt
      181 tttcagtgtc acattccgac gtcgaccgag tggcgcgtta cattgcgaat caggaagaac
      241 accatcgaaa acacacgttt gctgaagagt acgaactctt tgttaagcgg tacggcttgg
      301 aatggcgaga cgaaggaaac cgttgaaacg gtttcctgat atgttgccac ccgttcacct
      361 ggctaaagcc aggtgagaat gagatcagag gggtccgcag ttcaagccgg ctcctttcct
      421 gaagatctga cctgtcgaac gctcgtatcg ccgttcatct ggctaaaccc aggtgagaat
      481 gagatcagag gggcccgcag ttcaagccgg ctcctttcct gaagatctga cctgtcgaac
      541 gctcgtatcg ccgttcatct ggctaaagcc aggtgagaat gagatcagag gggtccgcag
      601 ttcaagccgg ctcctttcct gaagatcttg gtctgtccaa tgctcggaca gccgttcacc
      661 tggctaaagc caggtgagaa tgagatcaga gggtccgcag ttcgagccgc gtgccctggc
      721 gatctgctgc cctttctgga gatctgccgt cgaacggttg gttcctcctg ctttgcacat
      781 tcgctgtgcg tacgctggtt aagcgctgaa ggcgacttga ccggcgatga tggtgaggcg
      841 cacgttgcca ttcgtgtcca gcgcgacgag gtcggcgcgc ttgccggttt cgatcgaacc
      901 gcgagcattg ctgaggccga gcaggcgggc ggggttgctg gaggcaagct gcgcgagatc
      961 aggaaagcaa atgccaagcg aacgcatcat tcgcactccg tccagcatcg tgattactga
     1021 tcccgctatg ctgcccgccg cattactggt gcggccgttc ttgaccgata tcgtctcgcc
     1081 ccaaatttcg taatctccgt cgccttttcc agtcgcagcg atcgcgtcgc tgattagact
     1141 cacacctcgc gctcctttaa tcttcaccag taacttgagc attagcgggt cgagatgaat
     1201 gccgtcggcg atcatgtcga aggtgacatt atcgtgcgac aagccccaac caatcggccc
     1261 agggctgcga tgatgcagcg gcgacatcgc gttcatgaag tgtgtcatgt ggtgcgcgcc
     1321 ggcggccaaa gcctggtcca acacctcaac agtcgcgcga gtatggccga ttgagatcac
     1381 ccaaccgcgc cgcttcaact cgcgaacaag ttcgacccca ccagcgattt cgggcgccag
     1441 cgtgatcatg cgcaccgtct cttcgctcac aggcagacgg tctaaatccg aggctcccga
     1501 atacgtcttg aagtgctcaa cgtgcaacgc gccgcactgc gccgagttaa caaacggtcc
     1561 ttcgtaatgc agaccgagaa ttcttgcgcc aactccatca ggcgtaccca tcgcctgcga
     1621 cacagccgcc gccacatttc cataatccgc gtcagccgcc ggaacgagcg tcggcaacca
     1681 ggcggtcact ccctgtgacg ccaggtattc ggaaacgacc tgcaactcgc ttgccgtcgc
     1741 ttccatcacg tcgataccaa ccgcgccgtg agtgtgaaca tcaatgaaac ccggtagtaa
     1801 aatcaatcct gaaagatcca gaacttcgcc ttccgccgtc gccgaatcag caacagaggt
     1861 aatcaatccc gcgtcgacaa cgaccgtgcc gctttccatc acacggtctg gcagaacaac
     1921 acgcgcattc tttaacacca ccggagtctg ggccatattt gtgttcatgg ccggaattat
     1981 ataatgctta ggcacacatg attcgtttta ctccaagttt gctcccgctg cttctaattt
     2041 gcgccacggc accggcacaa cccccccgcc accaagcgga acgagtacgc acttactacg
     2101 aaacggcccg caaattcgaa gcagccggta actggaggca agcggaacaa acctggcgcg
     2161 ctgtgctcaa actcgccggc aacgacgctc gcgcctggac caacctcggc gtcgccctca
     2221 accgccagga caaaacggcc gaggcgttgg aagcctggaa acacgctatc tccatcgatc
     2281 caaaacttcc ggggccatac ttcaatctgg gactcacatt cgtccggcaa ggcgactacg
     2341 acaacgccat cgcgccactt cgccaggctc tttcgctagc gcccaacaac gacggcgcca
     2401 ggcgcgcgct ggcgatcgcg ttaatggccc aggaaaaatt tcaggaagcc agccgcgaaa
     2461 tcgcgcaact gctctttcgc aatccggaag acgcagcact cctcgaactc gcagccaaaa
     2521 gctttatgtc gcaacggcgt taccaggagg cggcagcagt gcttcagcgc cgcatacgcc
     2581 tgccaaacat gacagtcttt ttgtggagcc agtatggaga cgctctcgac ggcgccggcc
     2641 tgacaccgga agctctggaa gcttatcgaa aagcagtaga actggatccg gagtcaacgc
     2701 tcacacgcta cggactcggc tatctctact ggaagttgta ccgctatgac gacgccgaac
     2761 gcgagttatt ggaagtcttg cgtcgcgcgc caaacgatcc gcgtgcttcg tttacgcttg
     2821 gagatcttta cctcacacgc ggagatgcgc aacgcgcgct accgttgctg gaaacggcgc
     2881 gtgcagcata tccaaacgag tttgacacca gatttgcgct cggccgtgca ctaatcttaa
     2941 ccggcaacct ggatcgaggg attgacgaac ttcgcgcggc ggtcgcgctt gatgacggca
     3001 tcgctgatgg gcattttcaa ctcgggcgcg cgctaatcaa agcgggtcgg aacgaggaag
     3061 gaaaactgga attgcagaaa gcacagactt tgcacgacaa gcagcgcaaa aacgaagccg
     3121 agcggttgcg caaaaagctg ccgtgagttt tcacgcaaag gcgctaagaa acgcaaaggc
     3181 gcgaaggaag agataattaa gtctcccttt gcgccttggc gtctttgcgc gagttactct
     3241 tttttctaga acaacagctt ggcgccgaat tggaactcac gcggtaagcg agcgcctaac
     3301 ggcaaaccaa aagtcgacgt cgtcgtcaag gtagtaccgg atccggtgaa ctgcgtgtga
     3361 ttgaacacgt tgaatgcttc cgctcggagc tggatcttga atcgatcctc gttgtcgaag
     3421 tagatgttct tcgtcaacgt caggttcgtc tggttgcggc caaacaaacg agcgaatgcc
     3481 cgaggcgcgt tgccgtaagt acccgccagc ggcgcagtga aagcgcgtgg gtcgaagata
     3541 tacggcagtc ccgaaaccgg atcgatcgtt ccggctaacc ccgcattcgg atcggaaact
     3601 acgtttggat actgaccgat tgtggcggcg atgttgctcg cgccggacac cactctagcg
     3661 accggctggc ccgacgaaat gtcagtgatg ccggcaatct gccaaccacc aaggaaccca
     3721 cgcgcccacg cactcggatg tttgcggaag aacggcagtt catatacgta gctagcactg
     3781 aaaatgtgcg gacgcgaagt t
//
LOCUS       DASTEY010000394         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1211779, whole genome shotgun sequence.
ACCESSION   DASTEY010000394 DASTEY010000000
VERSION     DASTEY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211779"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1399
                     /locus_tag="VFO72_06920"
     CDS             230..1399
                     /locus_tag="VFO72_06920"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9479055.1"
                     /translation="MNITHLECGLCGLKHEARRLLNLCRDCGKPLLVRYDLDRAARTL
                     TKESLPARRTDLWRYREVLPVENDENIVSLGEGWTPLLRAANLGERVGVKELYIKDES
                     QNPTQSFKARGMAAAVSMAKELGVTKLAVPSAGNAAGALAAYAARAGLECFIFMPRDT
                     PRANVVECEQTGAHVTLMDGLITDCGAEVGRRKDAEGWFDVSTLKEPYRVEGKKTLGY
                     ELAEQLNWELPDVIVYPTGGGTGLIGMWKAFAEMEQMCWIGSKRPRMVTVQAAGCAPI
                     VRAFEAGKRFADEFPNAATTASGLRVPKAIGDFLMLDALRDSGGTAVAVTDEELIAAT
                     REIGSLEGVFCAPEGAACLPALRKLIDAGQVSASDRVVLFNTGSGVKYMESFESV"
     gene            1424..2704
                     /locus_tag="VFO72_06925"
     CDS             1424..2704
                     /locus_tag="VFO72_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479056.1"
                     /translation="MRYLSCLLILLFCAPLVSAQQPWLTDSERARFEELRRSGLDALY
                     NIDYDKAQRDFKEIVQLYPHHPGGYQLLAARLWIKTLYESRRLQASLYSSESFYSNGD
                     DKVDPKIITEFRNLTREARRLAEAKLKKEPKNIEALDFLANTEGLKAAFEEAVERRHF
                     AALRNGNDAVNHHREVLKLDPTYIDAQITIGLYEYVVGSLPLPIKIIAGATGFRGSKK
                     RGLKMLEQVAQEGRWAQDDAKSVLVLLYTREKRFADVVRVTRELSAKYPRNYLLRLET
                     ADALVSLAAQKRKQKDLAGAVQAEKEAFASFDEMLRDRDVRETAGRALDLIHFKYGEA
                     LLTAGYNERAAKEFLATTKVDRAEPGLVTMAHLYAARAYDVAGKREEALAQYKEVLSR
                     PNVYDAHEEAKRGMKQAYKAEAIARTTEVESSSN"
     gene            2709..3905
                     /locus_tag="VFO72_06930"
     CDS             2709..3905
                     /locus_tag="VFO72_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9479057.1"
                     /translation="MANLGSNGSAGVTRPRRLLLCLDGVPHKLIEAARHRGLFDRFGP
                     PTRLLSPFPTMTNVALSAMFGATPPAGYESLYFDRTAGELRGGIRKYLGRRTPDKIPS
                     SYMDELDYQEPLPFEFLIYVAPETIWRTDMQKFRERFRNAPQTRDYFAFLKATDGLLH
                     SQGPARLAIALESLDKILKEIQSYCGAETEIVLFSDHGMNLEENKRVPLRSALQRRDL
                     EVVIPAFGLCSYIAVYCRDEQRIPDAAVASAEVTGVDFAAYKDGDTVVVESDRGRARI
                     ESQGESFKYSVVTGDPLELSRFGEGFKTDKEWFEQTAGHRYPDAVPNIYKSVFTPRVQ
                     HTADILISTKDGYYYGWSPFGRFVRLLATHGNALQSSSNAFLMSTHRQFPEYVRADDA
                     KALLRG"
     gene            complement(3967..>4218)
                     /locus_tag="VFO72_06935"
     CDS             complement(3967..>4218)
                     /locus_tag="VFO72_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1629 domain-containing protein"
                     /protein_id="HET9479058.1"
                     /translation="SIFNVLNMIDALDEGAINRFPEGKEILDIKLFVFRQSQVHGVDV
                     FKLPQQPLGRVFVTDKFVEILNMGGLVGFKFKWVWGSDR"
BASE COUNT          948 a         1080 c         1279 g          911 t
ORIGIN      
        1 ggagaacttg atgaactgat cgacgcgatt cgtgctggag ctacttatgt gaatgttcac
       61 agcacgagat ggccaactgg cgaaattcgc agccagatcg atggtaacag cggccacggc
      121 ggccactaag cagcatcccg cgagacgttt ttgctccgag tgcaagggga ttactgcatc
      181 cagtacgtga ccccttgcac attctctagt cctgccgtag taagaaccca tgaacattac
      241 ccatctcgaa tgcggcctct gcgggctgaa gcacgaggca cgtcgcttgc tcaacctttg
      301 tcgcgactgc ggcaagccac tgctggttcg ctacgatttg gatcgcgccg cgcgcacctt
      361 gactaaggag tccctgccgg cacgccggac ggatctgtgg cgctatcgag aggtgctgcc
      421 ggttgaaaac gacgaaaaca ttgtttcact tggcgaaggt tggacgcctt tgcttcgggc
      481 tgcaaactta ggcgagcgcg ttggcgtcaa agaactttac ataaaagatg agtcgcagaa
      541 tccgacacaa agttttaagg cccgcggcat ggcggcggca gtttcgatgg ccaaagaact
      601 cggcgtcacc aaactcgccg tgccctctgc cggaaacgcg gcgggcgccc tcgctgccta
      661 tgcggctcgc gccggacttg agtgtttcat ctttatgcct cgagacacac cgcgagcgaa
      721 cgtagtcgag tgtgaacaaa ccggcgcgca cgtcacgctc atggacggtc tgattaccga
      781 ctgcggcgcc gaggtcggaa gacgaaaaga tgcggaaggc tggtttgacg tttcgacctt
      841 gaaagaaccg tatcgggttg aaggcaagaa gactttaggt tacgaactcg ccgagcagct
      901 caattgggaa ttgccggacg tgattgttta tccaaccggc ggcggcactg ggctgattgg
      961 gatgtggaaa gcgttcgccg agatggaaca gatgtgttgg atcggcagca agcgcccgcg
     1021 gatggtgacg gtgcaggcag cgggttgtgc accgattgtg cgcgcgtttg aagcagggaa
     1081 gcgttttgcg gatgaatttc ccaacgcggc gacgaccgcg tccgggttgc gagtgccgaa
     1141 agcgattgga gattttttga tgctcgacgc gttgcgagat tcggggggaa cggctgtcgc
     1201 cgtcaccgat gaagaactga ttgcggcgac gcgtgaaatc ggttcgcttg agggagtgtt
     1261 ttgtgcgcct gaaggggcgg catgtttgcc ggcgctgcgc aaactgatcg atgcaggtca
     1321 ggtgagtgcg agcgatcgag tggtgttgtt caacaccggg tccggcgtga agtacatgga
     1381 gtcgtttgaa tccgtgtaat gggtgtcgaa gagaggtaac aaagtgaggt atcttagctg
     1441 tcttcttatt ctcttgtttt gtgctcccct tgtttctgct caacagccgt ggcttaccga
     1501 ttcggagcgc gctcgttttg aagaactgcg acgcagtggt ctcgacgcgc tctacaacat
     1561 cgactacgac aaagcccaac gcgatttcaa agagatcgtt cagctttacc cgcaccatcc
     1621 gggcggatac cagctattgg ccgcgcgtct gtggatcaag acactgtacg agtcgcgccg
     1681 gttgcaagcg tcgctctaca gttctgaatc gttctattcc aacggcgacg acaaggtcga
     1741 tccaaagatc attacggagt ttcgcaacct gacacgcgaa gccaggcggc tcgccgaagc
     1801 gaaactcaaa aaagaaccca agaacataga agcgctcgat tttctcgcaa acaccgaagg
     1861 tctgaaagcc gcgtttgagg aagcggttga gcgccggcat ttcgcagcgc tccgcaacgg
     1921 caacgacgct gtcaatcatc atcgcgaagt gttgaagctc gatccgactt acatcgacgc
     1981 gcagattacg atcgggctct atgaatacgt cgttggctca ttgccgttgc caatcaagat
     2041 tattgcgggc gcgaccggat ttcgcggttc gaagaaacga gggttgaaaa tgctggagca
     2101 ggtggcccag gaaggacgct gggcgcagga cgacgcgaag tcggttttgg tcctgctcta
     2161 cacgcgggaa aaacgctttg ctgacgtggt gagagtgacg cgtgagttga gcgcgaagta
     2221 tccgcggaac tatctgctgc gactggaaac ggccgacgcg cttgtttcgc tggcggcgca
     2281 aaaacgcaag cagaaggacc tcgcgggagc tgttcaagct gagaaagagg cgtttgccag
     2341 tttcgatgag atgcttcgcg atcgcgacgt gcgcgagact gcggggcgcg cgctcgatct
     2401 gattcacttc aagtatggtg aagcgttgct cacggcgggt tacaacgagc gggcggcgaa
     2461 ggagtttttg gcgacgacta aagtcgatcg cgctgagccg ggtttagtca cgatggcgca
     2521 tctgtacgcc gctcgtgctt acgacgtggc gggcaagcgc gaagaagcgt tagcgcagta
     2581 caaggaggtg ctctcacgtc cgaatgtcta cgacgcgcac gaagaggcga agcgcggcat
     2641 gaagcaggcg tacaaggcgg aggcgattgc gcgtacgacg gaagtggaga gcagtagtaa
     2701 ttaaatccgt ggctaacctg ggtagtaatg gtagtgctgg cgtgacgcgt cctcgtcgtc
     2761 tgctgctgtg ccttgacggc gtacctcaca aactgattga agccgctcgc caccggggat
     2821 tgttcgacag gtttggacca ccgacacggc ttctctcacc gtttcccacg atgaccaacg
     2881 tcgcgctgtc ggcgatgttt ggcgctacac cgccggcagg ttatgagagt ttgtatttcg
     2941 atcgcacagc gggtgagtta cgcggcggca tcaggaagta tctcggtcgc cgcacgccgg
     3001 acaagattcc ttcgtcttac atggacgaac tcgattacca ggagccgctg ccgtttgagt
     3061 ttctgatcta cgtcgcacct gaaacgatct ggcgcacgga catgcagaag tttcgcgagc
     3121 gttttcgcaa cgcgccgcag acgcgcgact actttgcgtt tctgaaagcg actgatggtt
     3181 tactgcacag ccaggggccg gcgcggctgg cgattgcgtt ggaatcgctc gataagattc
     3241 tcaaagagat tcagagttac tgcggcgcgg agacggagat cgtgttgttc tcggaccacg
     3301 ggatgaatct cgaggagaac aaacgcgtgc cgttgcgatc ggcgctgcaa cgcagagact
     3361 tggaagtggt gattccggca tttggtctct gtagctacat tgcggtctat tgccgggacg
     3421 agcagcgtat tcccgatgcg gcggttgcga gcgcagaagt aaccggagtc gatttcgccg
     3481 cttataaaga tggcgacacc gtggttgtgg aaagtgatcg aggtcgggcg cgaatcgaat
     3541 cgcagggcga gagtttcaag tattcggtgg tgacgggaga cccgctggag ttgagccgct
     3601 ttggagaggg atttaaaacg gataaagaat ggttcgaaca gactgcggga catcgttacc
     3661 ctgatgcggt accgaacatt tacaaatcag tcttcacacc gcgtgtgcaa cacaccgccg
     3721 acattctgat cagcacgaag gacggttatt actacggctg gtcccccttc ggccgcttcg
     3781 tacgcctctt agcaacgcac ggcaacgcgc tgcaatcaag ttcgaatgca ttcctgatga
     3841 gcacgcaccg acagtttccg gagtatgtgc gcgctgacga tgcgaaggcg ctgttgcgcg
     3901 gctaacctcg cttctgaagt gatctgccgc ttgagcgttt ggcttgtttc aaaacggtgg
     3961 taaagatcac cgatcagatc cccacaccca cttgaactta aatccaacta aaccacccat
     4021 gttcaagatt tccacaaact tgtcggtcac aaaaacacga cccaatggtt gctgcggcag
     4081 tttgaaaacg tcaacgccgt gaacttgtga ttgtcgaaag acgaagagtt tgatgtctaa
     4141 aatctctttc ccttcaggaa atctgtttat agccccctca tccaacgcat cgatcatgtt
     4201 cagaacgtta aaaatgga
//
LOCUS       DASTEY010000395         6764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1228525, whole genome shotgun sequence.
ACCESSION   DASTEY010000395 DASTEY010000000
VERSION     DASTEY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228525"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="truB"
                     /locus_tag="VFO72_06940"
     CDS             complement(<1..560)
                     /gene="truB"
                     /locus_tag="VFO72_06940"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9479059.1"
                     /translation="MRYRRAGPVARQGTRLAQFLSADDKEYDAIIRLGYATDTGDRTG
                     TPIDGPKEKKLGNWDEQEIEAALASLRGWIDQVPPMYSAKKREGKKLYELARRGEEVD
                     REPVRVCIHEFTAIKSDGQLIKDNLDGTYDFRVHVVCSSGTYVRVLAADFGRRLSVGA
                     HLAELRRTRVGDFRVENAVTLDDLKM"
     gene            complement(500..676)
                     /locus_tag="VFO72_06945"
     CDS             complement(500..676)
                     /locus_tag="VFO72_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479060.1"
                     /translation="MEGLLVIDKPAELTSHDVVARVRHILHERRIGHTGTLDPFATGV
                     LVLLLGRALAWLNF"
     gene            729..1211
                     /locus_tag="VFO72_06950"
     CDS             729..1211
                     /locus_tag="VFO72_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET9479061.1"
                     /translation="MNSDDVRRRTFNRAIKLLTVKPRSVAELRERLLQGKNTNAAIVE
                     TVIARLEEYGYLNDERFAFSYASLKVKQRPVGRRRLERDLKLKKVENKVAEGALELVY
                     AETPEEQLIDAAIEKRIRLRGKPKTRIEAKKLFDHLLRQGFEFELVSDKVRALFDPSC
                     "
     gene            1224..3920
                     /gene="alaS"
                     /locus_tag="VFO72_06955"
     CDS             1224..3920
                     /gene="alaS"
                     /locus_tag="VFO72_06955"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET9479062.1"
                     /translation="MTGNEIREIFLKYFEEHGHTRVRSSPLLPANDPTLLFTNAGMNQ
                     FKDTFLGREKRDYVRAASSQKCVRAGGKHNDLDEVGKTARHHTFFEMLGNFSFGDYFK
                     QDAIKYAWDLLVNVLKLDPKRLWFTVYGGDEEVAPDEDAERFWEEVGAPRDRILRFGR
                     KDNFWQMGETGPCGPCSEIHYYMGESPDDPENSADNVNGPGDTIMEIWNLVFMQFDRS
                     EVEPAKYKLEPLPAPSVDTGAGLERLAVVLQGVKSNYDTDLIRPIIDRTAKLADRHYE
                     PETQEGFAMRVIADHARATAFSIADGILPGNEGRNYVLRKIMRRAIYQGHHTLSLEGS
                     FFNQVTDFVVDLMGDAYPELKTQRDFIAKMVRLEEERFRSTLTVGLNKLDELFTSTKG
                     AMPDFKSLAKLYDTFGTPRDLIRVGLEERGFAIDEEEFNRSFDAALQELQQTGVTTKQ
                     DTKSAVNPVYEAVAVRTPKSVFKGYETTNVSDAKVVAVIKGDEVESLNKGDKGEIVLD
                     RTPFYAESGGQVGDVGLLVGPSRELALVHDTYSPVQGLIVHKVEIAEGRFKVGDTVSA
                     EVDVEKRDATRRNHTATHLMHAALREVLGSHVKQAGSVVAPTYLRFDYSHYQPLTQAE
                     KEEIERLVNYHILRNEPVQTDEMAIEEAMRSGAMALFGEKYGEKVRVLSIKGLEGVFS
                     KELCGGTHVRATGDIGLFKIVSDESIASGVRRIRAITGTDAYDRFREAEVLVDELSSG
                     LRTSRAELVNAVSRLQEELKKARREADGLRLKLASGSVGTSTNGDESREIAGVKVLAR
                     EASGLDTASMRQLSDTLLARIKSGVVILGRSSEGKVSLIVRTSADLTSKVPAGQVIKE
                     LAPIVGGRGGGKADMAEGGGNQPENLNAALQKSYEVVERLLA"
     gene            complement(3989..4324)
                     /locus_tag="VFO72_06960"
     CDS             complement(3989..4324)
                     /locus_tag="VFO72_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET9479063.1"
                     /translation="MKKFLTLTLLLCLFTTGAITQAATTSTQTGTKQGARRGPVFRAT
                     KEQINQAQAILKARGFYSGEQIGKLDEATRDGLRKYQQAEGLKVTGTLNKVTLEKMNI
                     ALTEKQKSM"
     gene            4518..5264
                     /locus_tag="VFO72_06965"
     CDS             4518..5264
                     /locus_tag="VFO72_06965"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9479064.1"
                     /translation="MRKSLLLLIFTATFAASAQAQSSSGYQIDNFDLPNAVKIQSPKV
                     EPRNQPKRLLRLTSMPLNPSLGGFTTGSSDIDNFIVESGKRNSVDPLLLYSIMHQESS
                     FKPRAMSHKGARGLMQLMPPTAVRFGVRNIWDPKQNIEGGARYMRFLLDLFDGDVRLA
                     LAGYNAGEGAVMKYGNKVPPYSETKEYVRRISRRYSMIRDPEAAASAGQLTTEQLAAA
                     KQKEPAPLNVYERSVFMARLPDGRLQLLSQ"
     gene            5391..5969
                     /locus_tag="VFO72_06970"
     CDS             5391..5969
                     /locus_tag="VFO72_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9479065.1"
                     /translation="MTCSVCGAYVTPSVRFCSNCGAPVDAEQTRIARLQSRSLEPTVD
                     SELERTIFTVRPTMIFIKAGYALAVLGGIALVFLLASIGLPASMSIPLALALLLIPAY
                     YHVRRNMIRYTVTDAKLQIDTGLIARTTRNIPLSKVQDVTVSASIPQRLLGFGDIVVD
                     NASELGGSTVMHNISKPRHYADLLLRELRRWQ"
     gene            5979..>6764
                     /locus_tag="VFO72_06975"
     CDS             5979..>6764
                     /locus_tag="VFO72_06975"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HET9479066.1"
                     /translation="MLHIHLVISVLLLFVLPAESGKFRRVKSGGELSPQAKLDTESAP
                     VQTEQAEYVAANGERFSVEIVRFRTDGEAYELLSLVAAEARAKEPVAIENTPGTAGII
                     SSDQVAFFKGTHFVRITSVKPVVTPANLTALAQSLSDTLDKSDGEIPVLIKHLPSPDE
                     SQKNAVFLNNFNTLTSIAPQQPVLTAVEGDGNADAALANVGSSKVLVVEFKTPQLATD
                     NDKRIVARIQELWRLGQPAPTAYRRVGNYSVFVFDAPDEQTAKE"
BASE COUNT         1529 a         1876 c         1905 g         1454 t
ORIGIN      
        1 ctcattttca agtcgtccag cgtgaccgcg ttctcaactc tgaagtcgcc gactcgcgtg
       61 cgacgcaact cagccaaatg tgcgccgacg gacagacgcc ttccaaaatc tgcggcaagc
      121 acccgcacat acgtgcccga agagcaaaca acgtgcacgc ggaaatcgta ggtgccgtcc
      181 aggttgtctt tgatcagttg accgtctgac ttgatagcgg tgaactcatg aatgcaaaca
      241 cgaactggct cacggtccac ctcctcgccg cgtcgcgcaa gttcgtagag ctttttgccc
      301 tcacgcttct tggctgaata cattggtggc acctgatcta tccaaccacg caggctcgcc
      361 agcgctgctt cgatctcctg ttcgtcccaa tttccgagct tcttctcttt tggaccatca
      421 attggggtcc cagtccgatc gccggtatcc gtggcatagc ccaaacggat gatcgcgtcg
      481 tactccttgt cgtccgcgct cagaaattga gccaggcgag tgccctgccg agcaacagga
      541 ccagcacgcc ggtagcgaat ggatcgagcg tgccggtatg accgatgcgc cgttcgtgaa
      601 gaatgtgccg cacgcgcgca actacatcgt gagacgtaag ttccgccggt ttgtcgatga
      661 ctaacagtcc ttccatgcaa acattttaga ttgcgaactg ccgattggcg atgtgagaat
      721 cggtccgcat gaactcagat gatgtacgcc gccgaacatt caatcgcgcc atcaaactgc
      781 tgaccgtcaa accgcgttcg gtggccgagc tgcgcgagcg gttactgcaa ggaaaaaata
      841 ctaatgcggc tatcgtggag acggtcatcg cacggctcga ggagtatggc tacctcaacg
      901 acgagcgatt cgctttcagt tacgcgtctt taaaagtgaa gcagcggccc gttgggcggc
      961 ggcgtctgga acgcgacctg aagctgaaga aagttgaaaa caaagtggcg gaaggcgctt
     1021 tggagctggt gtacgccgaa acaccggaag aacagcttat tgacgcagcg atcgagaagc
     1081 gcatccgcct gcggggaaaa cccaagacac ggatcgaagc aaagaaactc ttcgatcatc
     1141 tcctgcgaca ggggtttgag tttgaactcg tctcggacaa ggtacgtgca ctcttcgatc
     1201 cgtcttgtta aactcaaacg gacatgactg gtaacgaaat acgcgagatt tttttgaaat
     1261 acttcgagga gcacggccac acgcgcgtgc gctcctcgcc acttctgccc gccaacgatc
     1321 cgacgttgct gttcaccaac gcgggtatga atcagttcaa agacacgttt ctgggccgcg
     1381 aaaaacgcga ttacgtccgc gctgcgtcgt cgcaaaaatg tgtgcgcgcc ggcggcaaac
     1441 acaacgacct ggatgaggtc ggtaaaactg cgcggcatca cacgtttttc gagatgctcg
     1501 gcaatttttc gtttggcgat tactttaaac aggacgcgat caagtacgcg tgggatttgc
     1561 tggtcaacgt tctgaaactc gatccaaagc gtctgtggtt cacggtctac ggcggcgatg
     1621 aagaggttgc tcctgacgaa gacgcagagc gcttctggga agaagtcggc gcaccgcgcg
     1681 acagaatctt gcgcttcgga cgcaaagata atttctggca gatgggtgag accggaccgt
     1741 gtggtccatg cagcgagatt cactactaca tgggcgagag tcctgacgac ccggaaaact
     1801 ccgcggacaa cgtgaacgga ccgggcgaca caatcatgga gatctggaat ttggtcttca
     1861 tgcagtttga tcgcagcgag gtcgaaccag ccaagtacaa actcgaaccg ctgccggccc
     1921 cctcggtcga tactggcgcc ggtctcgaac ggctcgcggt ggtgttgcaa ggcgttaaat
     1981 caaattacga cacggatttg atccggccca tcatcgatcg gacagcgaaa cttgccgatc
     2041 gccactacga gccggaaacg caggaaggct tcgccatgcg tgtcatcgcc gaccacgcgc
     2101 gcgcgaccgc gttctcgatt gccgatggca tcctgcccgg caacgagggg cgtaattacg
     2161 tcctgcgaaa aatcatgcgc cgtgcgatct atcaaggtca ccacacgctc tctttggaag
     2221 gttcattctt caaccaggtc acagacttcg tcgtcgatct gatgggcgac gcttatcccg
     2281 aactcaaaac tcaacgcgac tttatcgcaa agatggtgcg actggaggaa gagcgttttc
     2341 gttccacgtt gacagtgggc ttgaacaagc tggacgagtt gttcacgtca acaaaaggag
     2401 cgatgccgga tttcaaatcg ctcgcgaagc tctacgacac gtttggaact ccgcgcgacc
     2461 tgatccgcgt gggtctggaa gagcgaggat ttgcgatcga cgaagaagag ttcaaccgct
     2521 cgtttgatgc ggcgctgcaa gaactgcagc aaacaggcgt caccacgaaa caggacacca
     2581 aatccgcagt caatcctgtt tacgaggcgg tcgcggtacg aacgcctaaa tccgtgttta
     2641 agggctatga aactacgaac gtttcggacg caaaggtcgt cgccgtcatc aagggcgacg
     2701 aggtcgaatc gctgaacaaa ggagataaag gcgagattgt tctcgatcgg acgccgttct
     2761 atgcagagag tggtggacaa gttggcgacg ttggcctact ggtcggtccg tctcgtgagt
     2821 tggcgcttgt tcacgacacg tattcaccgg ttcagggttt aatcgttcac aaggtagaaa
     2881 tagctgaagg tagattcaaa gttggtgaca cggtttctgc tgaagtcgac gtcgaaaagc
     2941 gcgacgccac tcgccgcaat cacaccgcga cgcatctgat gcacgcggca ctgcgcgagg
     3001 ttttaggctc gcacgtcaaa caagccggtt ccgtcgtggc gccaacctac ctacgcttcg
     3061 actactcgca ttaccaaccg ttaacccagg cggagaagga agagattgaa cgcctggtca
     3121 actatcacat tctgcgcaac gagccggtgc aaaccgacga aatggcgatc gaggaagcta
     3181 tgcgctccgg tgcgatggcg ctctttggcg agaagtacgg cgagaaggtc cgtgtgcttt
     3241 cgatcaaagg actcgaagga gtcttttcaa aagaactctg cggcggtacg catgtgcgcg
     3301 ccaccggcga catcggactg ttcaagatcg tcagcgatga gtcgatcgct tcgggagtgc
     3361 gaaggattcg ggcgatcacc ggcacggatg cttacgatcg atttcgtgaa gcggaagtgc
     3421 tggttgacga acttagcagc ggcttgagaa cgtcgcgcgc ggagctggtc aatgctgtgt
     3481 cgcgtttgca ggaagagctg aaaaaggcac ggcgtgaagc cgatggcctg cgtttgaaac
     3541 ttgcgagtgg cagtgtggga acgtcgacca atggcgacga gtcgcgcgag attgctggcg
     3601 tgaaggtctt ggcgcgcgag gcaagtggac tcgacacggc ttcgatgcgg caactatccg
     3661 atacgcttct ggcgcggatt aaatctggcg ttgtgatact cgggcgcagt agtgagggga
     3721 aagtttcgtt gatcgtgcgg acttctgctg acctgacttc taaagttccc gccggtcagg
     3781 tgatcaagga gcttgcgccg attgtgggcg gacgcggtgg cggcaaagct gacatggcag
     3841 aaggcggcgg caatcaaccc gagaacctta atgcagctct tcagaagagc tatgaagtcg
     3901 ttgagcgcct tttggcctag gtagttttgg acaggattac aggatcggca ggattgtcct
     3961 gttaatcccg tgaaccctgt ccaactcgtc acatgctctt ctgtttttca gtgagagcga
     4021 tgttcatctt ctccagggtc accttgttca gggtgcctgt taccttcagc ccttcagcct
     4081 gctgatactt gcgcagtcca tcacgcgtcg cttcgtcgag cttcccgatc tgttctccgc
     4141 tgtaaaagcc gcgggccttg aggatcgcct gggcctggtt gatctgttct ttggtggcgc
     4201 gaaagacagg gccacgcctg gcgccttgct ttgtgccggt ttgggttgag gttgtagctg
     4261 cctgggttat tgctccggtc gtgaagagac agagcaacag tgtgagcgta aggaatttct
     4321 tcatctgttt tttcgtcctt tgtgaggcgc gctgaagcgc gcggtttgtg gttaaaacgg
     4381 gcctatcata catccgctgt caatagcccc ggctaagaac gcctgcccca gctaaaacgc
     4441 cagctttaac cgtgttggca agtgacaaac agccgtgcta ctattcctct acctcacgct
     4501 tgccctgaag cgaaaccatg cgtaaatccc tattgttgct catctttacc gccacttttg
     4561 ccgcctcggc gcaggcgcaa agctcttcgg ggtatcagat cgacaacttc gacctgccca
     4621 acgcggtcaa aattcagtcc ccgaaggtag aacccaggaa ccagcccaag cgcctgcttc
     4681 gtctcacctc aatgccactc aacccctcgc tgggcgggtt tactacagga agttccgaca
     4741 tcgacaactt tatcgtcgaa tccggcaaac gcaactccgt ggacccgctg ctgctctatt
     4801 ccatcatgca ccaggagtcg agcttcaaac cgcgtgcgat gtctcacaaa ggtgctcgtg
     4861 gcttgatgca gttgatgccg ccgactgctg tcagatttgg cgtcagaaac atctgggatc
     4921 ccaaacagaa catcgagggc ggcgctcgtt acatgcgttt tctgctcgat ttgtttgacg
     4981 gcgatgtgcg actggcactg gccggttaca acgccggcga gggcgcagtg atgaagtatg
     5041 gcaataaggt gccgccgtac agcgaaacga aggagtacgt gcgccgtatc agccggcgtt
     5101 acagcatgat tcgcgatccg gaagccgcgg cgagtgcggg ccaactcacg acagagcagc
     5161 tcgcggccgc gaagcaaaaa gaaccggcgc cgttaaatgt ttatgagcgc agcgtcttca
     5221 tggcccgcct gcccgacggc cgcttacagc tactcagcca ataagccgcg taaatccgcg
     5281 cgacctaccc tacgaataca aggaggcggg aacctttagt ggtcgccgcc tcgttttgta
     5341 ttacacttag ccacggattt ggctgacaga ggtagcgggg ttaagaggaa atgacctgtt
     5401 cggtctgcgg agcgtatgta acaccaagcg tgcggttctg ttcgaactgc ggcgcacctg
     5461 ttgacgctga acaaacacgc atcgcgcgtc tgcaatcgag atctctcgag ccaacagtcg
     5521 attcagaact ggaacgcaca attttcactg tgcgtcccac tatgatcttc atcaaagccg
     5581 gctacgccct cgcagtgctc ggcggcatcg cgctcgtatt tctgctggca agcatcggtt
     5641 taccggcgtc gatgtctatc ccgctcgcct tggcattgct gctgatcccc gcctactacc
     5701 acgtccggcg aaacatgatt cgctacaccg tgactgacgc caagctccag atcgacaccg
     5761 gtctcatcgc acgcaccact cgaaacattc ccctaagcaa agttcaggac gtcactgttt
     5821 cagccagcat tccgcaacga ctgctgggct ttggtgatat cgtcgttgac aacgcaagcg
     5881 aactcggcgg ctcaaccgtg atgcacaaca tcagcaagcc acgccattac gccgaccttc
     5941 ttttgcgtga actaagacgc tggcagtaaa cttacgctgt gttacacatt catctggtta
     6001 tctccgtcct cttgttgttt gttttgcccg cggagtctgg gaagtttcgg cgcgtgaagt
     6061 cgggtggcga actgtcgccg caagcgaaac tcgatacaga atccgcgccg gttcaaacag
     6121 agcaggcgga gtacgttgcc gcgaacggcg agcggttctc ggttgaaatt gttcggtttc
     6181 ggacagatgg tgaggcgtat gaactgctca gcctcgtagc cgctgaagcg cgtgcgaaag
     6241 agccggttgc gatcgaaaac actcccggga ccgctggaat tatcagcagc gatcaggtcg
     6301 cttttttcaa gggcactcat ttcgtaagaa tcacgagcgt caagcccgtc gttacccccg
     6361 cgaatctaac tgccctcgct cagtcgctga gcgacactct cgacaaaagc gacggtgaga
     6421 ttccggtcct aatcaagcat ctgcccagcc cggacgagtc gcaaaagaat gctgtctttc
     6481 taaacaactt caacacgctt acgtcgatcg cgccacagca accagtgctc accgccgtcg
     6541 agggtgacgg caatgcagac gcggcgctcg caaatgtagg ctcgagcaag gtgctggtcg
     6601 tcgagttcaa aacgccgcaa ctcgcgacgg ataatgataa gcgcatcgtc gcgcgtattc
     6661 aggaactgtg gaggttgggc caaccggcgc caaccgctta ccgccgcgtt ggcaattatt
     6721 ccgttttcgt tttcgacgcg ccggacgagc agacagccaa agag
//
LOCUS       DASTEY010000396         7497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_123754, whole genome shotgun sequence.
ACCESSION   DASTEY010000396 DASTEY010000000
VERSION     DASTEY010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123754"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFO72_06980"
     CDS             complement(<1..259)
                     /locus_tag="VFO72_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479067.1"
                     /translation="MLIISRAFLFFSWAVQAAIVAIILLMILRLIGDAADLNPFAWTA
                     RTLRRLTDVFVVPVRGALRQVGIDPKFAPLVVILVSILLGYF"
     gene            complement(246..968)
                     /locus_tag="VFO72_06985"
     CDS             complement(246..968)
                     /locus_tag="VFO72_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9479068.1"
                     /translation="MTRVDPAEGLAARFADVRARIAAAARSSGRAPEEVTLIAVSKTH
                     PPSLISRLIELGATDLGENRVQEAESKIPEVGRTMARWHLVGHLQANKARRALNLFDV
                     IHSLDSIDLARRLDRLCVEVERQTLPLLIQVDLGHEETKSGVDESELSQLAETVQQLE
                     RLELIGLMTLPPFFDDPKQARPYFRRLRELRDELARNGAFGERRGELSMGMTHDFEIA
                     IDEGATMVRVGTAIFGQRDADN"
     gene            1600..1686
                     /locus_tag="VFO72_06990"
     tRNA            1600..1686
                     /locus_tag="VFO72_06990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1634..1636,aa:Leu,seq:cag)
     gene            1877..2272
                     /locus_tag="VFO72_06995"
     CDS             1877..2272
                     /locus_tag="VFO72_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9479069.1"
                     /translation="MNVDQAALKETEDVRPVGANNPLPDRTVRLKLAERPDTDRIQRV
                     VDLADALLAEAETLARDKAFTEEATRLKPLDILGGISFYDEVQRFETHLIKMALAETG
                     GNQAKAARLLGIKATTLNSKIKLFNIDVG"
     gene            complement(2300..2728)
                     /locus_tag="VFO72_07000"
     CDS             complement(2300..2728)
                     /locus_tag="VFO72_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479070.1"
                     /translation="MTTDQKNLKLLYTLFCDDVRLEAGNKLSFMGVFQNIMVQQLPVS
                     LIKFAIVNHWQGQGSYLSEVRILTPDRQQPIVVSQPTRFEIANGAFADNISFFVNVTF
                     PTAGRYVVQTLIDSNLFQEELLTVSDVAEPEMLGTSEAVN"
     gene            complement(2735..4750)
                     /gene="ligA"
                     /locus_tag="VFO72_07005"
     CDS             complement(2735..4750)
                     /gene="ligA"
                     /locus_tag="VFO72_07005"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9479071.1"
                     /translation="MAAKATAREIEKLREEIRRHDELYYVNNAPEISDREYDELLERL
                     QKLEQAHPELITSDSPTQRVGGRPAEGFPEVVHSRQMLSLDNSYNIEELRAFDDRCRR
                     LAEGRPLDYVAELKIDGLSLSLQYADNLLVRGVTRGDGRIGEDVTQNARTIRSIPLRL
                     RREAKALDTNIEVRGEVFIPREVFEKTNAEREEAGEPRFANPRNAAAGAIRQLDSRLV
                     ARRKLDMFAYDLFAGGGRKPFPTHWQALEWLQAAGFRVNPHRKVCKTIDEVIEFANEK
                     EALRDDLGYEIDGLVVKVNSTALQDEFGATQKAPRWAIAYKYPARQASTRVNDIFVSV
                     GRTGALTPVAMLEPVFLAGTTVSRATLHNEDEIQRLGVKIGDWVMIEKSGEIIPKVLS
                     VVTSKRTGKERDFKPPKECPVCGGLISRIEGEVVARCVAADCPAQLMGRLIHFASRRA
                     MRIEGLGESLAAQLVSERLVRDVGDLYSLTLDQVAGLERMAKKSATNLLDQIEASKNR
                     ELTNLIYALGIRHVGERTAGILAHEAGSLQRLAEMSVEELDAIPEIGLTVAESVRDWF
                     DDEGNRALCDRLRAAGVKTESERKSSASHDERFAGKQFVLTGTLASFTRDEARALIET
                     RGGRVNSSVSKKTDYVVAGEAAGSKLDKAQSLGVSVLDEEAFKAMLE"
     gene            complement(4844..6292)
                     /locus_tag="VFO72_07010"
     CDS             complement(4844..6292)
                     /locus_tag="VFO72_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9479072.1"
                     /translation="MSFIKFDRYVDRHARSFTERLQSLCRMPSVAARGTGMRAIADTV
                     EQLMQRVGIGTRTFRVGSGYPIIYGECGSGPKSFVIYGHYDVQPVGHLTEWSSGPFAA
                     TIQDGKLYARGAANGKGDLIARLAAVEAYQKTFGKLPVTLRFLIEGEDGLGSPSLYRF
                     TSEHAELLNANGCLWDEGGRDTKETPVVSLGFKGITFLELRAFGARSDLHSKWGTIVP
                     NPAWRLVQALATITSPKGVITIDGFSSHVAPISDEDAEALKSIELDESGLKREFRIGG
                     WVRSMKGQTLIKEHIFGATCTICGIHTGHTEAGAKTVLPSMAMARLDFRLVPDLTAEI
                     VVGLLREHLDVRGFKDIEIIELGSAPLAKSSAKSRIARAVVESAAEVYGRPAIVYPMD
                     PSSGPVGAVCGVSQPPTPVASFGTAYAGSNPHGPDENIRIDDFLQSIKLVGRVLHKVA
                     ETKSKPSAEFSVSEDATPTAPLPRTRHAEKKE"
     gene            complement(6289..7068)
                     /locus_tag="VFO72_07015"
     CDS             complement(6289..7068)
                     /locus_tag="VFO72_07015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9479073.1"
                     /translation="MEEIEIRECTTIEEFDSCVALQREAFGLPDMEISPRRHLIVSRQ
                     AGGWTLAAFVAGRMIGFVHQLAAVRPNNEIYGYSHMMAVTKDYQNKGIGARLKWAQRE
                     RALAEGRTLIKWTWDPMQARNAHFNLNRLGVIVDSFGDNFYGTDYFADPTQALEDRPG
                     LQSDRLFARWDLKSSRVAALGSGMSPAIEAKPVVSVSIPSEWNALVKRDTLHAREVQA
                     RVRSEFKEAFARGLVCAALERGDVESKYLLFEPQINTDRHG"
     gene            complement(7106..>7497)
                     /locus_tag="VFO72_07020"
     CDS             complement(7106..>7497)
                     /locus_tag="VFO72_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479074.1"
                     /translation="QQVKIDYEVAPEITTIVSDQGKLRQTLFNFIAWAVSRSPAGETV
                     KVAVDVDDNGILAIKVKDNGEPAKDTSGVFDPEHSNSAHEPNINELGIIIGHKLLELM
                     GGDVALQNCMPRGLEATITMPARPAKG"
BASE COUNT         1623 a         2260 c         1968 g         1646 t
ORIGIN      
        1 gaaagtaacc gaggagaatg gacacaagga ttacgaccag tggcgcgaac ttcggatcga
       61 taccaacctg gcgcaaagcc ccccgcaccg gaacaacgaa cacatccgtg agccggcgca
      121 acgtgcgcgc cgtccaggca aacgggttca ggtcagccgc gtcgccaatc aaccgcagga
      181 tcatgagcag gatgatcgcg acgatagccg cctgaacagc ccaactaaaa aatagaaacg
      241 cgcgactaat tatcagcatc tcgttgacca aaaatcgcgg tgccgactcg caccatcgtc
      301 gctccttcgt cgatcgctat ttcaaagtcg tgtgtcatac ccatcgacag ttcgccgcgg
      361 cgctcgccga aggcgccgtt gcgcgcgagt tcgtcgcgca gttcccgaag tctacgaaag
      421 tagggccggg cttgtttcgg atcgtcaaaa aacggcggca gcgtcatcaa gccgatcaac
      481 tcgagccgct cgagctgttg cacagtctca gccagttgcg acagttcgct ctcgtcgact
      541 cctgactttg tttcttcgtg acccagatcg acctggatga gcaacggcag ggtttgcctt
      601 tccacttcaa cgcacaagcg atcgagacgc cgggcgagat cgatcgaatc cagcgagtgg
      661 ataacgtcaa agaggttgag cgcgcgacgc gccttgttag cctgaagatg tccgacgagg
      721 tgccaccgtg ccatggtccg ccccacttcc gggatcttcg actctgcttc ctgaacgcgg
      781 ttctcgccaa ggtcggtcgc gcccaactcg attagcctgc ttatcagact cgggggatgc
      841 gtcttgctga cggctatcag cgtaacctcc tctggagcgc ggccactgct gcgggcagcg
      901 gcggcgatgc gggcccgaac gtccgcaaat cgtgcggcaa gtccttccgc tgggtcaact
      961 ctcgtcatgg cgacaatcgg atcgatacgg aatcataata ccctaacggg caaaaggagc
     1021 tagaagcgtc gccggctttg gtggtatggt agctgacagg agggagcttt atgaaactta
     1081 tggtttccag aaaattgata gggacagcgg tcctggcgct gctggtggtg acgatgtcca
     1141 ccgaggtact gggccagggt cgcaatcgga gagtgtttgg acgctcagac aagaagtgcg
     1201 ccaagtttgt taactgccac gatgcacgtg atggtcgttg ggatggtcgc ggaccgcggc
     1261 gcaacaacac ctcgctgacc agccgcatct tcagacgcac tcgacgaaat cgagacttag
     1321 atattaggaa cggacgcaga ggatcacgcc gggaccgcga tttcgatcgg aatcgaaact
     1381 ggagacgagg acgataggga caaaactctc cccttccgcc ttttttgccc cagtcacaat
     1441 tttcctctgg taggtatttt tactgacacc accgatataa actccgaaaa catcatcgga
     1501 caaaccctct aaacttaaca actcttaaca acttttccag caactctttc acggttgaca
     1561 ctcgtctaag ctcgttcgca taatgacaac ccgttccttg cggatgtggc ggaactggca
     1621 gacgcgcagg tctcagaagc ctgtgatggt gacatcgtgg aggttcaagt cctctcatcc
     1681 gcaccaaacg aaaagttttt tgcggcgggt ctgttaaccg gactcgccgt ttttcgtctg
     1741 taacacaagg gcgcgcggct ttgcacaaaa agtgtagcgc acagcagaag ctcttaaagg
     1801 cccaaaaata tttgtcacgg attttcaacg cccggtgttt tcgaagtaag aggtccagtc
     1861 tgaaagggga gtattaatga acgtcgatca agcagctctg aaggaaaccg aagatgtgag
     1921 acctgtaggt gctaacaatc cattgcctga cagaacggtg cgtctgaaac ttgcagagcg
     1981 gcctgatacc gaccgcattc aacgcgtcgt cgatctggct gatgctcttt tggcagaggc
     2041 ggagactctg gctcgtgata aagctttcac tgaggaggca accagactca aaccattgga
     2101 cattctcgga ggaattagct tttacgatga agtacaacgg tttgagactc acttgatcaa
     2161 aatggcgctg gccgaaaccg gaggcaatca ggcgaaggcg gccagactgc tgggcattaa
     2221 agcaaccact ctcaattcca aaatcaagct cttcaacatc gatgttggct agggccgacc
     2281 agccatcatt ttccagtttc taatttacag cttccgacgt cccgagcatc tccggctcgg
     2341 cgacgtcgga gactgtcagt aactcttcct gaaaaagatt cgaatctatc aacgtttgta
     2401 cgacatatcg tccagcggtc gggaaggtca cgttaacaaa gaagctgatg ttgtcggcaa
     2461 aggcgccatt agcgatctcg aagcgagtgg gttgcgacac gactatcggc tgctgtcgat
     2521 cgggcgtgag gatgcgcacc tcggataagt aactgccctg gccctgccag tgattgacga
     2581 tggcgaattt aattaaggag acagggagct gttggaccat gatgttctgg aacacgccca
     2641 tgaacgacaa cttgtttccc gcctcgagcc ggacgtcgtc gcaaaaaagc gtgtagagaa
     2701 gtttgagatt tttttgatcc gtagtcattt gtgtttactc caacatcgcc ttaaaggctt
     2761 cttcgtctag cacggatacg ccgagcgatt gcgccttgtc gagttttgag ccggccgctt
     2821 cgccggccac aacgtaatcc gtcttcttgc tcacggaaga attaacccgc cccccgcgcg
     2881 tttcaatcaa cgccctggcc tcgtcgcgag tgaaactcgc taaggttccg gtgagcacga
     2941 actgtttgcc cgcaaaacgt tcgtcgtgcg acgcgctgct tttgcgctcg ctttcggttt
     3001 ttacacccgc agcccgcagc ctatcacaaa gcgcgcgatt cccttcatcg tcaaaccagt
     3061 cgcgcacgct ttcagcgacc gtcaaaccga tctccgggat tgcatccaac tcttcgaccg
     3121 acatttcagc cagccgttgg agcgaacctg cttcgtgcgc cagaatgccc gcggtgcgtt
     3181 cgccaacatg acgaatgccc agcgcataga tcaggtttgt tagttcacgg ttcttgcttg
     3241 cttcgatctg atcgagcagg tttgtggccg acttcttcgc cattcgttcg aggccggcaa
     3301 cctggtccag cgtcagtgaa tagagatcac cgacgtctct cacgagacgc tcggacacga
     3361 gttgcgctgc cagcgattca cccaaaccct cgatccgcat cgcgcgacgc gaagcaaagt
     3421 gaatcaaccg gcccatcaac tgcgccgggc aatccgccgc cacgcaacgc gccaccactt
     3481 caccttcaat ccgtgaaatc agtccaccac agacaggaca ctctttcggc ggcttaaaat
     3541 ccctttcctt tcctgtgcgt ttcgacgtga cgacactcaa cactttggga atgatctcgc
     3601 cgctcttctc aatcatcacc cagtcgccga tcttgacgcc cagccgctgg atctcatctt
     3661 cgttgtgcag cgtggcacgc gaaacggtcg tcccggcaag aaacaccggt tccagcatcg
     3721 caacgggcgt gagcgctccc gttcttccca cggaaacaaa aatgtcgttg acacgtgtcg
     3781 acgcctgtcg cgcgggatac ttgtacgcga tggcccagcg cggcgccttc tgggtcgcgc
     3841 cgaattcatc ctgcaacgca gtggagttga ccttcaccac gaggccgtca atttcgtaac
     3901 ccagatcatc gcgaagcgcc tccttctcgt tcgcaaactc aatgacttca tctatggtct
     3961 tgcaaacctt gcgatgggga tttacacgaa atcccgctgc ctgaagccat tcgagcgctt
     4021 gccagtgcgt cggaaacggc ttacgcccgc caccggcaaa caagtcgtaa gcaaacatgt
     4081 cgagcttacg acgtgcgacc agacgcgagt cgagttgccg gatcgcgccc gcggcggcgt
     4141 tgcgcggatt cgcaaaccgc ggttctcccg cttcttctcg ttccgcgttg gtcttctcaa
     4201 acacctcgcg cggaatgaac acttcgcctc gcacttcaat atttgtatcg agcgctttcg
     4261 cttcgcgtct cagacgcagc ggtatgctgc gaatcgtgcg cgcattctgc gtcacgtctt
     4321 caccgatgcg gccgtcacca cgcgtcaccc cacgcacgag caaattgtct gcatactgca
     4381 acgacaaaga caaaccatcg atcttgagct cggcaacgta gtcgagcggc cgcccttccg
     4441 ccaatcgccg gcaacgatcg tcaaacgcgc gcaactcttc aatgttgtaa ctgttgtcca
     4501 gggacagcat ctgccggcta tggaccacct cgggaaaacc ttcggcggga cggcccccaa
     4561 ctcgctgcgt cggactgtca gatgtgatta attcgggatg ggcctgctcg agcttctgca
     4621 agcgttcgag cagttcgtcg tattcgcgat cggagatctc gggagcgtta ttgacgtaat
     4681 aaagttcgtc atgacgtcga atctcttctc gtagcttctc gatctcgcgt gcggttgcct
     4741 tcgcggccaa aagttactcc ttcttctcct gggaccgcag gcgtctcgtc tgcatcaccc
     4801 ggttaaggct cgatcggacg cgggcgagac gtgcgcgctc cagtcactcc ttcttctctg
     4861 cgtgtcttgt gcgcggtagc ggtgcggtag gcgtggcgtc ttcgctcact gagaactcgg
     4921 cgctcggctt cgacttcgtc tccgccactt tatgaagcac gcgcccgacg agcttgatgc
     4981 tctggaggaa atcgtcgata cgaatgtttt cgtccgggcc gtgcggattc gaacccgcgt
     5041 acgccgtgcc gaaagacgcc accggcgtcg gtggctgcga cactccacaa accgcgccca
     5101 ccggtccact cgaaggatcc atcggataaa ctatcgccgg ccggccataa acttccgccg
     5161 ccgactccac caccgcacgc gcaatacgcg actttgccga cgacttcgcc agtggcgcgc
     5221 ttcccaattc gatgatctcg atatctttga aaccacgcac atcgagatgt tcgcgcaaca
     5281 gtcccaccac gatctcggcc gttagatccg gaacgagccg aaaatcgaga cgtgccatag
     5341 ccatgctcgg caacaccgtt ttcgcgcctg cttcggtatg gccggtgtga atgccgcaga
     5401 tagtgcaggt tgcaccaaag atgtgctcct tgatcagcgt ctggcccttc atcgatcgca
     5461 cccaaccgcc gatgcgaaac tcgcgtttca gtccggactc gtcgagttcg atactcttca
     5521 gcgcttcagc gtcttcgtcg ctaatcggag caacgtgcga agaaaaacca tcgatggtga
     5581 taacgccttt gggtgaggtg atcgtggcga gtgcctgcac cagccgccac gcgggattgg
     5641 gaacgattgt gccccacttg gaatgcagat ccgaacgcgc gccaaacgcg cgcagctcga
     5701 gaaaagttat gcctttgaag ccgagactca cgaccggagt ctctttcgta tcgcgcccgc
     5761 cttcgtccca caagcagccg ttagcgttca aaagctcggc gtgttcgctg gtaaagcgat
     5821 aaagactcgg actgccaaga ccgtcttctc cctcgatcag aaaacgtaaa gtgaccggca
     5881 gtttgccaaa cgtcttttga tatgcttcaa ccgccgcaag gcgtgcgatc agatcgcctt
     5941 tgccgttggc agcaccgcgc gcgtacaact ttccgtcctg gatcgtcgct gcgaatgggc
     6001 cggaactcca ttcagtcaga tggcccaccg gctgcacgtc gtaatggccg taaattacaa
     6061 agctctttgg gccactaccg cactcgccgt agatgatcgg ataaccggaa cccactctga
     6121 atgtgcgcgt gccgatgccc acccgttgca tcaactgctc gactgtgtcg gcgattgcgc
     6181 gcatgcccgt gccacgcgca gcgacggacg gcatccggca gaggctctgc aacctctcgg
     6241 taaagctgcg cgcgtgacga tccacatagc gatcgaactt aataaaactc atccgtgtct
     6301 atctgtgttt atctgtggtt caaacagcag atatttcgac tccacgtctc ctcgttccag
     6361 agccgcgcaa accaatcctc gtgcaaacgc ttctttaaac tctgacctga cacgcgcctg
     6421 gacctcgcgt gcatgcagcg tatcgcgctt cactaacgca ttccactccg acggaatcga
     6481 cacggacaca accggtttcg cctcaatggc cggcgacata ccggagccca acgccgccac
     6541 acgcgacgat tttagatccc agcgtgcgaa gagccggtcg ctttgtagtc ccggccgatc
     6601 ttccaaagcc tgcgtaggat cagcgaagta gtccgtacca tagaagttgt cgccgaagct
     6661 atccacgatc acgcccaaac ggttcaggtt aaaatgcgcg tttcgcgcct gcatcgggtc
     6721 ccaggtccac ttgatcaagg ttcttccttc ggcgagcgcg cgttctcttt gggcccactt
     6781 caaccgcgcg ccgatacctt tgttttgata gtctttcgtc acggccatca tgtgcgagta
     6841 accgtagatt tcattgttcg gccgcactgc ggcgagttga tgaacaaaac cgatcatccg
     6901 gccggcgaca aacgccgcca gcgtccaacc gcctgcttgt cgcgacacga tcaagtgacg
     6961 gcgcggagag atctccatat cgggcaggcc gaaggcctcg cgctgaaggg caacacaact
     7021 gtcaaactct tcgatcgtcg tgcactcacg aatctcaatc tcttccatgc gtaacggttt
     7081 tgtcccttcg cctccgtacc tttcgctatc cttttgcggg ccgcgccggc atcgtgatcg
     7141 ttgcctcgag accacgcggc atgcagttct gcaacgcaac gtctccgccc atcagttcga
     7201 gcagtttgtg accgatgatg atgccgagtt cattaatgtt cggctcgtga gcggaattgc
     7261 tgtgttcggg atcgaaaact cccgaggtgt ctttcgccgg ctctccgttg tctttcactt
     7321 tgatcgccag gattccatta tcgtcgacgt caacagcgac ttttacggtt tcgcccgccg
     7381 gactgcggct aacggcccag gcgatgaaat tgaagagcgt ttgccgcagt ttcccctgat
     7441 cggaaacgat ggtcgtgatc tccggcgcca cttcgtaatc gatcttcact tgctgct
//
LOCUS       DASTEY010000397         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_651351, whole genome shotgun sequence.
ACCESSION   DASTEY010000397 DASTEY010000000
VERSION     DASTEY010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651351"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VFO72_07025"
     CDS             <1..1019
                     /locus_tag="VFO72_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9479075.1"
                     /translation="AIAADVNQYAITWGSKDFRDAIARKTRSYLGLDIDPETEITVTC
                     GSTEGMIAAMMATVDPGEEVIVFEPFYENYAPDAILSDARPRHVPLRAPDWSFDRDEL
                     RQTFNEKTKAIIVCNPNNPTGKVFTQDEMEFIAGLCQEYDALCFTDEIYEHILYPRES
                     ADIRHISMAQIEGMRDRTVIVNSMSKTYSVTGWRVGYCIAPPEITSGIRKVHDFLTVG
                     AAAPLQVAGAYALSLPQEYYDTLQSEYRARRDLLLPVLEAAGFKTFVPDGAYYIMTDI
                     SGFGFSDDIEFTKHLIRDIGVACVPGSSFYSAPSLGQQQVRFCFCKKDETLHAAADRL
                     FKLK"
     gene            1070..1936
                     /locus_tag="VFO72_07030"
     CDS             1070..1936
                     /locus_tag="VFO72_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479076.1"
                     /translation="MAIAAQQLPDPEFNTSVANPAYNKNGPRVLFDEAHHNFHTTDGR
                     YKPFVDLLMNDGYRIIRNRQPFTKSSLSSFKVLVIANALGAEEMDDNGADGSAFTEEE
                     CQAVQEWVKSGGALLLIADHAPFGGAAAALASRFGVDMSKGFAYDQANAAPNSPSLLH
                     FSRENNLLATHPITEGRSQNERLNRVQSFTGQSLKGPDNSFAILKLSDTAKDTPNREA
                     DSSISAAGRAQAIALKFGKGRVVVQGEAAMLSAQIAGPNKNPMGMNVPGNDNKQYALN
                     VMHWLSGLLKEK"
     gene            complement(2124..3020)
                     /locus_tag="VFO72_07035"
     CDS             complement(2124..3020)
                     /locus_tag="VFO72_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479077.1"
                     /translation="MRAQLFKSVLFGTVVAALVAVSIMAQSGNRPHATASGGVQVGVV
                     ARRDDSRPTPITSKEVSVYDNGVEQSIRNFTPDPSPARIVLLVDNSLSIRADVEKLEQ
                     AAREFAYEIYEGDKLLIVGYDEQAEIVSDWTDDAKAIETSLKLFRKKGQPHLFDAIRA
                     VVEEALRPLPIQKRIIVVISDGLDRGSKATFEQTLAELQAENIMVYAIQAVDRTRGAI
                     RRDVPKPKVVIDKLAEGTGGQIFSIDEPAVAAKAICDELRKNRYLLSYVPSSAPFGTP
                     RALLVIGNQGITVRAKSMQQPN"
     gene            3594..5705
                     /locus_tag="VFO72_07040"
     CDS             3594..5705
                     /locus_tag="VFO72_07040"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9479078.1"
                     /translation="MSNTAQSLSLERKIRMHEAREGIINRLAMTLPHAINLDRFLTVI
                     VSELGRMMNVDRCDVIQLRSASELRISHEWRTSNDVPSSLGTVIPLDLKQFSEHVDIR
                     KPIRLDDISAVGLNQRVRFLAQSLGTRSLLVVPVMLGEDVLGLVGLHTTGTPREWLDD
                     EISFLQSIAGQIAIGYQYTRLYTDKEREAETKRALLSIANALNARSDFREVSSLVLER
                     AIALVGADYSALGVLDPAVSRISLAAFKAAPHATTDSVQGLFDSHGQSLDVTAFPAMV
                     EVLAEGKTLRLLETDLPLPIRFLFNSVLGGRAALVAPVRVGGQTFGLLGLVWSEARER
                     FDDHEVALVEGIADQIGTALERDQLSAEIMHLRSALHERYGEDRIIGQTPAIRRAIEL
                     ALSVADTQTTVLIEGESGTGKELLANLIHFNSGREDQPYVKLNCGAIPETLLESELFG
                     HEKGAFTDARALRRGRFEEANGGTLFLDEVGEMSLSAQVRLLRVLQDGEFTRVGGSEV
                     IKTDVRVLAASNVDLKRAVELGTFRQDLFYRLSVFPITLPPLRERPEDIHPLVIHFLE
                     YYKQKTGRFISGISKDALQALITYEWPGNVRELENAIERAVIIASGRQIELEDLPEAI
                     SKIALRERDRVKVERAKAASEGRTTTFEVTVPSSMEEIERLAIEATLDYTAGDKSHAA
                     RALGIGRKTLYRKLEQYNGGS"
     gene            5923..>6141
                     /locus_tag="VFO72_07045"
     CDS             5923..>6141
                     /locus_tag="VFO72_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9479079.1"
                     /translation="MGYDEYRRDSGDSISTRLTYLLIGGGIGAILALLFAPKSGHELR
                     GDIADATRKGIDRSREAAQQLGDRAGEYY"
BASE COUNT         1316 a         1720 c         1738 g         1367 t
ORIGIN      
        1 aggcgatcgc ggccgacgtc aaccagtatg ccattacctg gggctcaaaa gactttcgcg
       61 acgcgatcgc ccgcaagacc cgttcgtatc tcgggctcga tatcgatccc gaaacggaga
      121 tcaccgtcac ttgcggttcg accgaaggca tgatcgccgc aatgatggcg acagtcgatc
      181 ccggcgaaga agttatcgtc ttcgaaccct tctacgaaaa ctacgctccc gacgcgatcc
      241 tttcggatgc gcgcccgcgc cacgtgccgt tgcgcgctcc ggattggagt ttcgatcgcg
      301 acgagttgcg ccaaacgttc aacgagaaga ccaaagcgat catcgtctgc aaccccaaca
      361 atccgacggg caaggtcttc acacaggacg agatggagtt catcgccggt ctctgccagg
      421 agtacgacgc gctctgcttc actgacgaga tttacgaaca cattctttac ccgcgcgaaa
      481 gcgctgacat ccgccacatc tcgatggccc aaatcgaagg catgcgcgat cgaactgtga
      541 tagtcaattc gatgtcgaag acctactccg ttaccggttg gcgcgttggt tactgcatcg
      601 cgccgccgga gatcacgagc ggaattcgca aggtccacga ttttctgaca gtcggagccg
      661 cagcgccgtt gcaggttgcg ggagcttacg cgttgtcgct gccgcaggag tactacgaca
      721 cgttgcaaag cgaatatcgg gcgcgtcgcg atctgttgct gcctgtgttg gaggcggccg
      781 gtttcaaaac ttttgtgccc gacggcgctt actacatcat gaccgacatc tccggcttcg
      841 gcttttcgga tgacatcgag tttacgaaac acctgatccg cgacatcggc gtggcctgcg
      901 ttcccggctc cagtttttac agcgcgccct cgctgggcca acagcaggta cgcttctgtt
      961 tctgcaagaa ggacgagacg ctgcacgccg cagccgatcg cttattcaag ctcaaatgaa
     1021 acgctcggct actatttgcc ttctcctgtc cgttttgttc gtgtcatctg tggccatcgc
     1081 ggcccagcaa ctccccgatc ccgagttcaa cacgtccgtc gccaacccgg cttacaacaa
     1141 gaacggaccg cgcgtgctgt tcgatgaagc tcatcacaac ttccacacga ccgacggccg
     1201 ttacaaaccg ttcgtcgatc tgttgatgaa cgacggctat cgcatcattc gtaaccggca
     1261 gccattcacg aaaagttctt tgagcagttt caaagtgctg gtgattgcga atgcacttgg
     1321 cgcggaagag atggacgaca acggcgccga tggttccgcg tttacagaag aagagtgtca
     1381 ggcggtccag gagtgggtga aaagcggcgg cgcgttactg ctcatcgcgg atcacgcgcc
     1441 attcggcggc gctgcggcag cactcgccag ccggttcggc gtcgatatga gcaaaggttt
     1501 cgcttacgat caggcaaacg ccgcccccaa cagtccttca ctccttcatt tctcgcgtga
     1561 gaacaatctg cttgccacgc accccattac tgaaggccgc agtcaaaacg agcgcctcaa
     1621 tcgcgtgcag agtttcaccg gacaatcgct gaaaggtccg gacaacagct tcgcgattct
     1681 caaactttca gacacagcga aagacacgcc aaatcgtgaa gcagactcat caatctccgc
     1741 tgcggggcgc gcccaagcga tagcgctgaa gttcggcaag ggacgtgtcg tggtccaggg
     1801 cgaagccgca atgctctcgg ctcaaatcgc cggaccaaac aaaaacccga tgggtatgaa
     1861 cgtgcccggc aacgacaaca aacagtatgc gctgaatgtg atgcactggc tgtccggcct
     1921 tttgaaagag aagtaatgcc cggatgggat ctaaagcgga aggttatatg gatcgtaggc
     1981 ggactgcttg tcggaacata cgtagcttac tctgactcat ttctcgacga cggcactttc
     2041 gttccgcgat ttttcatttt tatggaagca atggtcgtgc tgattatggc aggacttttc
     2101 tatttgtact cgcggaagaa aagttagttg ggctgctgca tcgacttcgc cctgaccgtg
     2161 atgccctgat ttccgatgac gaggagagcg cgcggcgtcc cgaacggtgc gctggatgga
     2221 acgtaggaga gcaagtaacg gttctttcgc agttcgtcgc aaatagcctt cgccgcgacc
     2281 gcaggctcat cgatcgaaaa gatctggccg ccggttcctt ccgccagttt atcaatcaca
     2341 actttcggtt tcggcacgtc gcgccggatc gcgccgcgtg tacgatcgac tgcctggatc
     2401 gcataaacca tgatgttttc cgcctgcaac tcagccagcg tttgctcaaa cgtggccttg
     2461 cttcctcgat ccagtccgtc actgatcacg acgatgattc gtttttgaat cggcagcggc
     2521 cgcagtgctt cttcaaccac cgcgcgaatc gcgtcgaaaa gatgtggctg ccccttcttt
     2581 ctgaataact tcaacgacgt ttcgatcgct tttgcgtcgt cagtccagtc ggaaacgatc
     2641 tccgcttgtt cgtcatagcc gacgatgagc aacttgtcgc cttcgtagat ctcgtaagca
     2701 aactcgcgtg cggcctgttc gagtttttcc acgtccgcgc gaatcgatag tgaattatcc
     2761 accagcagga caatacgcgc cggcgacggg tcgggggtga aatttcggat tgactgttcg
     2821 acgccgttgt cgtaaacaga aacttcttta ctggtgatgg gcgtggggcg actgtcgtcg
     2881 cgcctggcaa cgacgccgac ctgcacgccg cctgaagcag ttgcgtgtgg ccggttgcct
     2941 gactgggcca tgatcgaaac ggccactagt gcggcgacaa cagttccaaa cagtactgac
     3001 ttaaacagct gggcgcgcat ttactttgct aactggcttc aatggggcgg cttcaatcgc
     3061 cagtggatgc tttcggaggt gaagccttct ccgaagtttt ctttgctggg gcgctgtcct
     3121 ttgtactctt cttatcagtc gtttccgagg tttcagacgc tgtttgggat tcggacttct
     3181 cgcctttcgt tgcctttgcg gcgtagtcag tgacgtacca accagaaccc ttgaactgaa
     3241 tcgccggcgc cgaaattcgt ttttctaaac ggccgccaca cttgcggcac ttaaccagcg
     3301 gcttgtccga aactttctga aacacttcaa tgtgagcctt gcacttgcga cattcgtatt
     3361 cgtaaatcgg catgttcgga ttatacggac aaaaggaagt cgtgtcctaa tttgcgaagg
     3421 ctctttcctg ggaccgcagg cgtctcgcct gcactgagcg cgaagcgcga aaaacgagcg
     3481 atgcaggcga gacgcctgcg gtcccaggaa agagcgtgtt cacctcttgg gacaccccca
     3541 aagcaggctg ctttgcaact ttccacggcc ttcgtctatg ctgtcgcgag acaatgtcta
     3601 acaccgctca atcgctttcg ttagaacgca agatcagaat gcacgaggcg cgcgagggca
     3661 tcatcaaccg cctcgcgatg actctcccac acgccatcaa tctcgaccgc ttcctgaccg
     3721 tcatcgtctc ggaactcggc cgcatgatga acgtcgatcg ctgcgacgtg attcagttga
     3781 ggtcagccag cgagctgcgc atcagccacg agtggcgcac ttcgaacgac gtgccgtcga
     3841 gtttgggaac tgtaattccg ctcgacctca aacagttttc cgaacacgtc gacattcgca
     3901 agccgattcg tctcgatgac atctcggcgg tcggactcaa ccagcgagtc aggtttctgg
     3961 cgcagagtct gggcacgcgc tcgttgctcg tcgtgccggt aatgctgggt gaggatgtgc
     4021 ttggtttagt cggcctgcac acgacgggca cgccgcggga gtggctcgat gatgaaattt
     4081 cgtttttaca gtctatcgcg ggtcagattg cgatcggtta ccagtacacg cgtctataca
     4141 cggacaagga acgcgaagcg gaaacaaaac gcgccttgtt gtcgatcgcg aatgcgttga
     4201 atgcgcgctc agattttcgt gaagtgtcgt cgctggtctt ggagcgcgcg attgcgctgg
     4261 tcggcgccga ttattccgcg ctcggcgtgc tcgatccggc tgtaagccgc atcagcttgg
     4321 cggctttcaa agccgcgccg cacgcgacga ctgattccgt gcagggactg tttgacagtc
     4381 acggtcagtc gctggacgtg accgcgtttc cggcgatggt tgaagtgctg gcagaaggca
     4441 agacattgcg attgctcgaa accgatctgc cgttgccgat tcgatttctg ttcaattccg
     4501 tgctcggtgg acgcgcggct ttggtggcgc cggttcgggt gggtggacag acgtttggtc
     4561 tactgggttt ggtttggagt gaagctcgcg agcgtttcga cgatcacgaa gtcgcgttgg
     4621 tcgaaggcat cgcagaccag atcggcacgg cactcgaacg cgaccagttg tcggccgaga
     4681 tcatgcacct gcgcagcgcg cttcacgaac gttacggcga agatcggatc atcggtcaaa
     4741 cgcccgcaat tcgccgcgca atcgagctcg cgttgagtgt cgctgacacg caaacgacgg
     4801 ttttaatcga aggcgaatcc ggtactggaa aggaattgct ggcgaacctg atccacttca
     4861 attcgggccg cgaagatcag ccttacgtga aattgaactg cggcgcgatt cccgagacat
     4921 tgctcgagtc ggagctgttt ggacacgaga agggcgcgtt tactgacgcg cgggcactgc
     4981 gacgcggccg gtttgaagaa gcaaacggcg gaacactgtt tctcgacgaa gtcggagaga
     5041 tgagtttgtc ggcgcaagtg cgtctgttgc gtgtgttaca ggacggcgaa tttacgcgcg
     5101 tcggcggcag tgaagtgatc aagactgacg tacgcgtcct cgccgcttcg aatgtggatc
     5161 taaaacgggc ggtcgaacta ggaacgttcc ggcaggacct gttttatcgt ttatctgttt
     5221 ttccgattac tctgccgccg ctgcgcgaac gcccggaaga cattcatccg ctcgtgatcc
     5281 acttcctcga gtattacaaa cagaagactg gccggtttat ctccggcatc tcgaaggatg
     5341 cgttgcaggc attgattact tacgagtggc ccggcaacgt gcgagaactc gaaaacgcga
     5401 tcgagcgagc ggtaatcatt gcttcgggac gccagatcga gttggaagat ctgccggagg
     5461 cgatcagcaa gatcgcgttg cgcgagcgcg accgcgttaa ggtcgaacgt gccaaagccg
     5521 cgagtgaagg acgcacgacc acattcgagg ttacggttcc ctcgtcaatg gaagagatcg
     5581 agcgccttgc aattgaggct actctggact acaccgcagg cgacaagtcc cacgccgctc
     5641 gcgcgctcgg catcggacgc aagacgctct atcgaaagct ggagcagtac aacggcggct
     5701 cctgatattg tcgattgcca attggcgtca ttgccaattt ccgattgccg atttccgatt
     5761 tatgagcggt cggcattaca caaattggca attggcaatc ggcaatcggc aatttcccca
     5821 attggcaatc agaaataggc aatgttatat tccaacgcct cgtagtcttt ggagaccagc
     5881 gaagtaatag ggcttcgctt tgaagagaaa ggacccatca agatgggata cgacgaatat
     5941 cggagagata gcggtgacag catctctaca cgcttaacat atctgctcat cggcggtggt
     6001 atcggcgcga ttctggccct gctgttcgcg cccaaatcgg gccatgaact gcgtggcgac
     6061 attgcggacg cgacccgcaa aggtatcgat cggtcgcgtg aggcggcgca acagttgggc
     6121 gaccgcgccg gcgagtacta c
//
LOCUS       DASTEY010000398         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1360264, whole genome shotgun sequence.
ACCESSION   DASTEY010000398 DASTEY010000000
VERSION     DASTEY010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360264"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1003
                     /locus_tag="VFO72_07050"
     CDS             179..1003
                     /locus_tag="VFO72_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479080.1"
                     /translation="MAPQLSPETQRDFETKLKEARELYEKDSRSADGVIWLGRRTAYL
                     GRYKEAIAIYSKGIEQFPSDARLYRHRGHRFITLRCFDAATADLEKASKLIKGRADET
                     EPDGLPNARNVPTSTLQSNIWYHLGLAYYLKGDFQRALSAYREAEKVSSNADMLVATT
                     HWLYMTLRRLGREKEASETLLKIKDDLDVIENADYYKMINVYKGKVKVQDLWNEISKD
                     SNSLSNATLGYGVGNWFLYNGERAEAEKVFRQVTAGNQWASFGYIAAESELNRNRR"
     gene            1217..1780
                     /locus_tag="VFO72_07055"
     CDS             1217..1780
                     /locus_tag="VFO72_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET9479081.1"
                     /translation="MASVQGSLFTELDGFRYQPDLLDHSEEEVLLAHLRALPFREFEF
                     HGYTGKRRVVSFGWKYEFSGEKLRKVEDIPQFLLPLRDRAAGFAGLKPEAFEHALVTE
                     YGPAAGIGWHRDKAVFGQVVGISLLAPCILRFRRKAGEKWERVNISAEPRSAYHLTGP
                     ARSEWQHSILRVDSLRYSITFRTVREG"
     gene            complement(1777..2283)
                     /locus_tag="VFO72_07060"
     CDS             complement(1777..2283)
                     /locus_tag="VFO72_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479082.1"
                     /translation="MFFDQRSLPVFVSKHATLGVGGGMVRLKANGEDVVAEAVYFTRG
                     LPNGIGGAVIVGDYLYGTEVGSKLLAVEFATGKVMWESADFGHASVAAADGHLYLHLL
                     KGDYVLVEATPQGFREKGRFMPPNPPKHKQVGTYPEMAFTYPVIANGRLYIPDLGTLW
                     AYDISEKR"
     gene            complement(2390..3508)
                     /locus_tag="VFO72_07065"
     CDS             complement(2390..3508)
                     /locus_tag="VFO72_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9479083.1"
                     /translation="MLRTIRHKFTGPLGLILVLSVCLTLFSPVILSAQRATPPAQPAS
                     GPGGKQYAHTSVTKNRYGKGGEDYWIFEPDAPKPASAPVIVFLHGWGGMNPLYYGAWI
                     DHLVKRGNIVIYPRYQASLLTSVREFTPNTLSAVKDALKRLQTEPGHIKPDLKKFATV
                     GHSLGGLLAASVAALASRHGLPRVSAVMSVEPGITEAPINIPLADLKKIPPETLLLAL
                     AGDQDTLVRDSDAKRIYYESSRVPASNKDFVTMVSDTHGMPGLQASHRAPTAMDKSYD
                     SGEGFTGGPGESSDRAGGLPTRRVDGRQIARRERLETMVVNALDFYGTWKLFDALCDA
                     AFYGRNREYALGNTPQQRFMGVWSDGVPVKELKITDNP"
BASE COUNT          797 a         1042 c          954 g          769 t
ORIGIN      
        1 gcttcgcgct cagtgcaggc gagacgcctg cggtcccagg aaagagcgtg ttcacaaatt
       61 gggacaccat ccttttgtgc aaagctatgg gctaatgaaa actctttgcg tcattctgct
      121 ttgtctcttc agctcttctg cttttgcaca aaccaattgt ctgcaaaccg cgcgcgaggt
      181 ggcaccacaa ctctcacctg aaacacagcg tgacttcgag acgaaactca aagaggcgcg
      241 agaactctac gagaaagatt cacgctcggc agacggcgtt atctggctgg gccgccgtac
      301 cgcctatctc ggacgttata aagaagcgat cgcgatctat tcaaaaggca tcgagcagtt
      361 tccctctgat gcgcggcttt accggcaccg tggacatcgt tttatcactc tgcgctgttt
      421 cgacgcggct acagcggatc tggaaaaagc gtcgaaactt atcaagggac gcgccgatga
      481 gaccgaaccc gatggtctgc caaacgcgcg caacgttccg accagcactc tccaatcgaa
      541 tatctggtat cacctcggtt tggcctacta tcttaaaggc gattttcagc gcgcgctcag
      601 tgcctatcga gaagcggaaa aggtctcaag caacgcggac atgctcgtgg ccacgacgca
      661 ttggctttac atgacgttac ggcgtttagg acgtgagaaa gaggcgagcg aaacgttgct
      721 caagatcaag gacgaccttg atgtcatcga gaacgcggat tactacaaga tgatcaatgt
      781 ctataaaggc aaggttaagg tgcaagacct ctggaacgag atcagcaaag actcaaacag
      841 cctgagcaac gccacgctcg gttatggcgt gggaaattgg tttctttaca acggcgagcg
      901 cgccgaagct gaaaaagtgt tccgccaagt caccgcgggt aatcagtggg ccagtttcgg
      961 ctacatcgcc gcggagtcag agttgaatcg aaatcgacgc tgacccgctt ggcgcgtggg
     1021 atacgcaaga aaagtgtccg caccagggcg ccgaaacgtc tgtcgcaccc tcggtgctgg
     1081 tcgagctgcc tatgattttc caggggctgc gcgctgcgcg cttgtccctg gctaccttct
     1141 ggacgcgcct tcggcgctcc gcccaatgct ggcggtaagg aactgtttgt ggttgttcgt
     1201 ttttgtcacg gattttatgg caagcgttca aggatcgttg ttcaccgaac tcgacggctt
     1261 tcgttatcaa cctgacctgt tggaccattc tgaggaggaa gtgctcctcg cgcacttgcg
     1321 cgcattaccg tttcgcgagt ttgagtttca cggttacacc ggcaaacgtc gcgtcgtttc
     1381 attcggttgg aaatacgaat tctccggcga gaaacttcgc aaggtagaag acatccccca
     1441 gtttctcctg ccgcttcgcg atcgcgcggc cggcttcgcc ggactgaaac ctgaggcatt
     1501 cgagcacgcg ctcgtcacgg agtatggacc agccgcaggc atcggctggc atcgcgacaa
     1561 agcagtgttt ggccaagtcg tcggtatctc actgctcgct ccgtgcatac tgcgcttccg
     1621 tcgcaaagcc ggtgagaagt gggagcgagt gaacatatca gctgagccgc gctcagccta
     1681 tcacttgact ggaccagcgc ggtccgagtg gcagcatagc atcctgcgag tcgactcgct
     1741 acgttactca atcaccttcc gcaccgtgcg cgaaggttag cgcttctcac tgatgtcgta
     1801 cgcccatagc gttcccaggt cggggatata caaacgaccg ttagcaataa caggatacgt
     1861 aaacgccatc tccggatacg ttccgacttg tttgtgcttc ggcgggtttg gcggcatgaa
     1921 gcgtcccttt tcgcggaagc cctgcggcgt cgcctccacc aagacgtaat ctcctttcaa
     1981 caggtgaaga tataaatggc cgtccgccgc cgccactgac gcgtggccaa aatccgccga
     2041 ctcccacatc accttccctg tcgcgaactc aactgcaagc agcttcgacc cgacttcagt
     2101 tccgtaaaga taatcgccga cgatcactgc gccgccaata ccgtttggca gtccccgcgt
     2161 gaagtaaacc gcttctgcaa caacatcttc gccattcgct ttcaaacgga ccatgccgcc
     2221 accaacaccg agcgtcgcgt gtttagaaac aaagacgggc aagctgcgct ggtcgaaaaa
     2281 cattcgcaaa gagtttggcg gtcaaccggt gaatgggcga ctagtgccgc actaccatgc
     2341 gatagcggat ttcaatcgcg acgacaacac tagaattgac acaattacgt cacggattgt
     2401 cggtgatttt caattctttc accgggacgc catcgctcca cacacccata aatcgctgct
     2461 gcggggtgtt gccgagcgcg tattcgcggt ttctgccgta gaacgctgcg tcgcaaagag
     2521 cgtcaaacaa cttccacgtt ccgtagaagt cgagtgcatt gaccaccatc gtctcgagtc
     2581 gctcgcgtcg tgcaatctgc cgcccatcga cgcggcgcgt cggcaaacca ccggcacgat
     2641 cggaactctc gcctggtcca ccagtaaaac cttcgccgct gtcgtaagac ttatccattg
     2701 ctgtcggcgc gcgatgactt gcctgcaatc cgggcatacc gtgcgtatct gaaaccattg
     2761 tcacaaaatc cttgttgctc gccggcacgc gcgatgattc gtaataaata cgcttcgcat
     2821 cactgtcgcg cacgagcgtg tcctgatctc ccgccaacgc gagcaagagc gtttccggtg
     2881 ggatcttctt cagatcggcc agcggaatat tgattggcgc ctcagtgata cccggttcga
     2941 cagacatcac cgccgaaact cgcggcagcc catgtctact cgccaacgcc gcaacactag
     3001 cagccaataa gccaccgagt gaatggccca ctgtcgcaaa cttcttcaag tcaggcttga
     3061 tgtgtcccgg ctcagtttgc agacgcttga gcgcgtcctt tacggcgctc aaagtgttcg
     3121 gcgtaaactc gcgaacacta gtcaaaagac tcgcttggta gcgcggatag atcacgatgt
     3181 tgccgcgttt cacgagatga tcgatccacg cgccgtaata caggggattc atgccgcccc
     3241 agccgtgcag aaacacgatg accggagccg acgcgggctt tggcgcgtcg ggttcaaaga
     3301 tccaatagtc ctcgccgccc ttgccgtagc gattcttggt cacagacgtg tgggcgtact
     3361 gttttccacc tggaccggag gccggttgtg ccggcggcgt ggcgcgttgc gctgagagta
     3421 tgaccggcga gaagagcgtc agacagactg acagaaccag gatcaagccc agcgggccgg
     3481 taaatttgtg ccggatggta cgtagcatgt cagttccctc cgctcctttg acgcacgaaa
     3541 accctaaaga gtaacccttt cc
//
LOCUS       DASTEY010000399         5829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1525389, whole genome shotgun sequence.
ACCESSION   DASTEY010000399 DASTEY010000000
VERSION     DASTEY010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525389"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFO72_07070"
     CDS             <1..406
                     /locus_tag="VFO72_07070"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET9479084.1"
                     /translation="GPRQRPDLAIHKFAKLISQGKPIPVFGDGSTRRDYTYVDDIIDG
                     VVAAITYNKSNYEVFNLGESRTVELRELISLLEKELDTHAIIDRQPPQPGDVPQTFAD
                     ITKARALLGYDPQTQIEEGLRRFVAWFRGKLF"
     gene            420..1433
                     /locus_tag="VFO72_07075"
     CDS             420..1433
                     /locus_tag="VFO72_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9479085.1"
                     /translation="MRVLDEQETLKQHAPPTHAADSPDVSVFLPVYNEEPNLRLLHEK
                     LDAALKSLGRSAEIVYVDDGSSDGSLKILRELAKEDPRVRVVALRRNYGQTAAMAAGI
                     DAARGKVLIPMDADLQNDPADIRRLLEKLEEGYDVVSGWRKNRKDKMVTRKIPSMIAN
                     RLISWIGGVPLHDYGCSLKAYRRESLQDVRLYGEMHRFIPIYASWAGARVTEIPVEHH
                     ARTMGKSKYGLSRTLKVVFDLMTIKFMASYQTKPIYVFGSFGMLAFAISLFAGLYAVF
                     LKVIHKADFVQTPLPILAIVMFAVGVQFLLMGLLAEMLVRTYHESQAKSIYAVRERLG
                     FKE"
     gene            1439..3370
                     /gene="asnB"
                     /locus_tag="VFO72_07080"
     CDS             1439..3370
                     /gene="asnB"
                     /locus_tag="VFO72_07080"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9479086.1"
                     /translation="MCGITGWANLDPHAPPPDGARDLLHAMCERMLHRGPDSEGLFVT
                     TGAALGMRRLAIIDLVTGEQPAFNEDKSVAVVLNGEIYNYRELRSSLEKRGHSFCSAS
                     DTEVLPHLYEEYGDEMVRELNGMFAFALWDSKRRRLLIARDRFGEKPLYWGVFDNTLL
                     FASEPKVLLAHPAVKPSLNLQALRQYLSFDYVPAPLSIYEGINKLPAAHTLTLENGQV
                     NVERYWRLSYKTTEPVPTEHEAAEQLRELLADAVRMRLVSDVPLGVLLSGGVDSSTVA
                     ALAVRASSEAVKTFSISFAEASFDESVYARGVAKYLGTDHHEERLSANLAANLVSEIG
                     SWMDEPFSDPSLVPTYLLSRFTRKHVTVALGGDGGDELFAGYPMYVGHRWAEVYKRVP
                     QVFRRGIVEPVVRLLPVKTKNLSFDYKARRFVTGAQYDNIARHHIWFGSFTPEQQEQL
                     LTPEALAASDGEIYAEARRIADECNNDDLVTQMQSVDTRLYLAEDILTKVDRASMAVS
                     LEVRAPFLDPRVAEFAASLPCNYKLRRMQTKYILKKAVQDMLPSFVTRRGKKGFGVPV
                     AEWLKVKLRPLARDLLSPERVRRAGVFNANYIERLQDEHERGVANHRKLLWTLLMFEL
                     WHESFVETPRRIETSVGSR"
     gene            3324..4547
                     /locus_tag="VFO72_07085"
     CDS             3324..4547
                     /locus_tag="VFO72_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479087.1"
                     /translation="MSKRRAASKRLSGAGEPQITQTLQKVYDQLLAWCREHDFAGHDP
                     FDALNSRVFQVTPFAKSRNARLLWTQLVKRSPADLRSLALVPAERNAKGLALFALAQL
                     PTFRRVRTEESISQARSLLSMLLSLKVEGFSGASGGYNFDWQSRNFYAPRGTPTVVPT
                     AFAARALIEAACEFREDEYLYSARSVCDFLLRDLPRTVDTATELCFSYAPDANTRIFN
                     ASLLAAEVLAAVGRLTNESSLRDYAERATRYVIKQQQPDGSWTYGADRNQSWIDNFHT
                     AYILFSLKRIISACSLGEEFQQSLGRGYRYWKENFFLADGWPKYYHDDPYPADAHAAA
                     SAIVTLLECRDLDETSATTARQVASWTIENLRDKRGFFYYQRRRFYTVRKPYMRWTQA
                     WMLYALARLLEDMPN"
     gene            4731..5030
                     /locus_tag="VFO72_07090"
     CDS             4731..5030
                     /locus_tag="VFO72_07090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9479088.1"
                     /translation="MEIEDECFIGHGVIFTNDVYPSAVNRDGTLQTEADWQVTKTKVK
                     TRASVGSNATIIAGVTIGEGALVGAGAVVTKDVPDFAIVTGVPARVIGDVRKRTT"
     gene            5027..>5829
                     /locus_tag="VFO72_07095"
     CDS             5027..>5829
                     /locus_tag="VFO72_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9479089.1"
                     /translation="MIRVGVIGYGYWGPNLVRNFMAAPGSAVTRVCDLREERLAPLRK
                     LYPAIKTCNQPADLINDSQIDAVVIATPVSSHFDLALAALKAGKHVLVEKPLAARTDQ
                     ARQLIDEAAARNLVLLVDHTFVYTDAVRKIRELIASGQLGEIYYYDAVRVNLGLFQHD
                     VNVIWDLAIHDLSIMDYVLPAKPVAISATGISHIPGQPENVAYITLFFSGAQIAHVHV
                     NWLTPVKVRHTLIGGSEKMILYDDLEPSEKLKLYDKGISVTPRQEDVYK"
BASE COUNT         1253 a         1606 c         1667 g         1303 t
ORIGIN      
        1 tggaccacgc cagcgtccgg atctcgcaat ccacaagttc gcgaaactga ttagccaggg
       61 caaaccgatt cccgtcttcg gtgacggctc cacgcgccgc gattacacgt acgtcgacga
      121 catcatcgac ggcgtcgtcg ccgcgatcac ttacaacaag agtaactacg aagtcttcaa
      181 cctcggcgag tcgcgcacgg tcgagttgag ggaactgatt tcgttgctgg aaaaggaact
      241 cgacacccac gccataatcg accgccaacc tccacagccc ggcgacgtcc cgcaaacatt
      301 cgcggatatc accaaagcgc gtgcgctgct cggctacgac ccacaaacgc agattgagga
      361 gggtctgcga cggtttgttg catggttccg cgggaaattg ttctaaaatg ctggacgcta
      421 tgagagtcct cgacgaacag gaaactctga aacagcacgc gccgccaacc catgcggcgg
      481 attcgcctga cgtctccgtc tttcttcccg tctataacga agaacccaat ctgcgcctgt
      541 tgcatgaaaa gctcgatgcg gcgctgaagt ctctcggacg ttccgctgag atcgtttacg
      601 tagacgatgg gtcgagcgac ggtagcctca agatcctgcg cgaactcgcg aaggaggatc
      661 cacgcgtacg tgtcgtggca ctgcgaagaa actatggtca aaccgcagca atggccgccg
      721 gcatcgacgc cgcgcgcggc aaggtgctga ttccgatgga cgccgacctg caaaacgacc
      781 ctgccgacat tcggcgactg ctcgaaaaac tcgaggaggg ctacgacgtc gtttccggtt
      841 ggcgcaaaaa tcggaaagac aaaatggtca cacgtaagat tccctcgatg atcgcgaatc
      901 gtttgatctc ctggatcggc ggcgtgccgc tgcacgatta cggttgctcg ttgaaagcat
      961 atcgtcgcga gtcgttgcag gacgttcgtc tctacggtga gatgcatcgt ttcattccga
     1021 tctacgcgtc gtgggcgggc gcgcgcgtca cggagattcc ggtcgaacat catgcgcgca
     1081 ccatgggcaa gtcgaaatat ggtttgtcgc ggacgctgaa ggtcgtattc gatttaatga
     1141 cgatcaagtt tatggcctcg tatcaaacga aaccgatcta tgtgttcggt tcgtttggta
     1201 tgttggcgtt tgcgatttcg ctgtttgccg gcttgtatgc ggtctttctc aaagtcattc
     1261 acaaggctga cttcgtgcaa acaccgttgc cgatcctggc gatcgtgatg tttgcggtgg
     1321 gcgtgcagtt tctgttgatg ggattactcg ctgagatgct cgtgcgcacg tatcacgagt
     1381 cgcaggcgaa atcgatttat gcggtgcgcg agcggctggg atttaaagag taattactat
     1441 gtgcggaatt accggctggg ccaatcttga tccacatgca ccgccacccg acggtgcacg
     1501 ggatctgctg cacgcgatgt gcgagcgcat gttgcatcga ggaccggatt ccgaaggtct
     1561 gtttgtgacg accggagccg cgcttggcat gcgtcgcctc gccatcatcg atctcgtcac
     1621 cggcgagcag ccggccttca acgaagataa atccgtcgcc gtcgttctga acggcgagat
     1681 ctacaattac cgcgaacttc gttcctcgct cgagaaacgc ggacattcgt tttgcagcgc
     1741 ttcagacacc gaagtgttgc cgcatctcta tgaggagtat ggcgatgaaa tggtccgcga
     1801 gctcaacggc atgtttgcct tcgctttgtg ggacagcaaa cgccgccggc tgctgattgc
     1861 tcgcgatcgt ttcggcgaaa aaccgctcta ctggggcgtg ttcgacaaca ctctgctctt
     1921 tgcctcagag ccgaaagtgc tgctggccca tcccgcggtc aagccttcac tcaacttgca
     1981 agcgctgcgg cagtatctgt cattcgatta cgtgcctgcg ccgctctcga tctacgaagg
     2041 catcaacaaa cttcccgcgg cgcacacgct cacgcttgaa aacggacagg taaacgtcga
     2101 acgctattgg cggcttagtt ataaaacaac tgaacctgtt ccaacggagc acgaagcggc
     2161 ggagcagttg cgcgaactat tggcagacgc ggtgcggatg cgtttggttt ctgatgtgcc
     2221 gctgggcgtt ttactttcag gtggtgtcga ttcgtcaacc gtggccgcgc tcgccgtgcg
     2281 tgcttcgtcg gaggcagtaa aaactttttc gatctcgttt gccgaagcgt ctttcgacga
     2341 atcggtatac gcgcgtggcg tcgcgaagta tctgggcact gaccatcacg aggagcgact
     2401 cagcgcaaat ctggctgcga accttgttag cgagatcggt tcgtggatgg acgaaccatt
     2461 cagcgatccg tcgctggtgc cgacctatct gctgtcgcga ttcacgcgca agcacgtaac
     2521 cgtggctttg ggtggcgatg gcggtgacga actgtttgcc ggctatccga tgtacgtcgg
     2581 tcatcgctgg gccgaggttt ataaacgtgt gccgcaggtg tttcgccgtg gcatagtcga
     2641 gccggtagtt cgtttgttgc cggttaagac gaagaacctt tctttcgatt acaaggcgcg
     2701 gcgatttgtg accggggcgc agtacgacaa catcgcgcgt catcacatct ggtttggttc
     2761 cttcactccc gaacagcagg agcagttact gacgcctgag gcgctggcag cgagcgacgg
     2821 tgaaatctac gccgaggctc gacgcatcgc agatgagtgc aacaacgacg atctggtcac
     2881 gcaaatgcaa agcgtcgaca cgcggttgta tcttgcggaa gacattttga cgaaggtcga
     2941 tcgtgcgagc atggccgtgt cgctggaggt gcgcgcaccg tttctcgacc cgcgtgttgc
     3001 tgagtttgca gcgtcgttgc catgcaatta caagttgcgt cgcatgcaga cgaagtacat
     3061 cttgaagaag gccgtgcagg acatgttgcc gagttttgtg acgcgacgcg ggaagaaggg
     3121 ttttggagtg ccggtggcgg agtggctgaa agttaaatta cgcccgctcg cacgcgatct
     3181 gttgtcgccc gagcgagtgc gacgggccgg tgttttcaat gcgaactaca tcgagagatt
     3241 gcaggatgaa cacgagcgcg gcgttgcgaa ccaccgcaaa ctgctttgga cgttgctgat
     3301 gtttgaactc tggcacgaga gctttgtcga aacgccgcgc cgcatcgaaa cgtctgtcgg
     3361 gagccggtga accacagatt acacagactt tgcaaaaggt ttacgatcaa ctcctcgcgt
     3421 ggtgccgaga acacgacttc gccggacacg accccttcga cgcactgaac agccgcgtgt
     3481 ttcaagtcac accttttgcc aagtcgcgta atgctcgtct gctttggaca caactcgtta
     3541 agagatcgcc cgcggatttg cgttcgctcg cgttggtgcc ggcggaacgc aacgccaaag
     3601 gtctcgcatt gtttgcattg gcccaactgc caactttccg ccgggtgcga actgaagagt
     3661 caatatcgca agcaaggagt ttgctgtcga tgttgttatc gctgaaagtg gaaggcttca
     3721 gtggtgcttc agggggttac aacttcgatt ggcagagccg caacttctac gcgccgcgag
     3781 gcactccgac tgtcgtgcca accgcattcg cagctcgcgc attgatcgaa gcggcctgcg
     3841 agtttcgtga agacgaatac ctgtactctg cgcgcagcgt gtgcgacttc ctgcttcgcg
     3901 atctgccgcg cactgtcgat actgccaccg aactctgctt cagctatgct cctgacgcca
     3961 acacgcgcat cttcaacgcc agcttactcg ccgcggaagt gctcgcggcc gttggcagac
     4021 tgactaatga gtcttcattg cgtgactatg cggaacgcgc gacccgttac gtcatcaaac
     4081 aacaacaacc cgacggctcg tggacctatg gcgccgatcg aaatcaatcg tggatcgaca
     4141 acttccacac tgcttatatt ctgttttcac tgaaacgaat aatctcggcg tgttcgctcg
     4201 gtgaggagtt tcaacagtct ctcgggcgcg gctatcgtta ctggaaggaa aacttctttc
     4261 ttgcggacgg ctggccgaaa tactaccatg acgaccctta tccggcggat gcgcatgctg
     4321 cagccagcgc aatcgttact ctccttgaat gccgtgacct ggatgaaact tcagcaacga
     4381 cggcccggca ggtcgcttcg tggaccatcg agaatctgcg cgacaaacga ggcttctttt
     4441 actatcagcg ccggcgtttc tacacggttc gcaagccata catgcgctgg acgcaggcgt
     4501 ggatgcttta tgcgctggcg cgtttgctcg aggacatgcc taactgaatg ccaatcgctt
     4561 ctgacgtaaa actcggccga gaggtccgca tttttcagcc tgatctggtg aacttatacg
     4621 gctgttcgat cggggacgaa acgaggattg gggctttcgt tgaggttcag aaaaacgcaa
     4681 tcataggagc tcgttgtaag ctttcgtcgc acagttttgt ttgcgagggc gtggagatcg
     4741 aagacgaatg ttttatcggc cacggcgtaa tattcactaa cgacgtctac ccgagcgctg
     4801 tcaatcgcga tggcaccctg caaactgaag ccgactggca ggtgactaag actaaagtca
     4861 aaacgcgcgc aagcgtcggc agcaacgcaa cgattatcgc aggcgtaacg attggagaag
     4921 gcgcactggt tggcgcgggc gcggtggtga ctaaagacgt gcctgacttc gcgattgtta
     4981 ccggagtgcc ggcccgcgtt atcggcgacg ttcgtaaacg cacgacatga tcagagtcgg
     5041 tgtaatcggc tacggctatt ggggaccgaa cctggtgcga aactttatgg ccgcgcccgg
     5101 ttcggccgtg acccgtgttt gcgatctgcg agaagagcgc cttgcgccac tgcgaaagct
     5161 ctatcccgca ataaaaacct gtaaccagcc tgccgatctc atcaacgatt cgcagatcga
     5221 tgcggtggtg attgccacgc ctgtctcatc gcactttgat ctcgcgctcg ctgcgttgaa
     5281 ggcgggcaag cacgtgctcg ttgagaaacc actcgcggcg agaaccgatc aggcgcgcca
     5341 gttaatcgac gaagcagcgg cgcgcaattt agtactgctc gttgaccaca cgtttgtcta
     5401 caccgacgcg gtgcggaaga ttcgtgaact gatcgcgtcg ggccagcttg gcgagatcta
     5461 ctactacgac gcggtgcgcg tgaatctcgg cctctttcaa cacgacgtca acgtgatttg
     5521 ggatcttgcg attcacgatc tctcgatcat ggattacgtc ttgcccgcca aaccggtggc
     5581 gatctccgcg acgggcatta gtcacattcc cggtcaaccg gaaaacgttg cctacattac
     5641 actcttcttt tccggcgcac agatcgctca cgtgcacgtc aattggctga ctccggtgaa
     5701 ggtgcgtcat acgctgatcg gcggcagtga aaaaatgatc ctttacgacg atctcgaacc
     5761 aagcgagaag ctgaaactct acgacaaagg aatcagcgtc acgccgcgac aggaagatgt
     5821 ctacaagat
//
LOCUS       DASTEY010000400         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_173867, whole genome shotgun sequence.
ACCESSION   DASTEY010000400 DASTEY010000000
VERSION     DASTEY010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173867"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1549
                     /locus_tag="VFO72_07100"
     CDS             215..1549
                     /locus_tag="VFO72_07100"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET9479090.1"
                     /translation="MDRWNSRSLFGDVGDATPETVPVEGAPLAERMRPRSLAEFVGQT
                     HLVGEGRILRQLLSRGGSLPSLILWGAPGTGKTTLARLLAEKSGMRFIALSAVFSGVK
                     DLREAIQEAKTEQRYGKRTVLFIDEIHRFNKSQQDALLHAVENGTVTLIGATTENPSF
                     EVTGALLSRCRVLVLNPLSEDEIKTVLQRALDDNERGLANYQPSISDTLLQRIAHTAA
                     GDARVALTALDTAVESTAPDANGVRTISEQTVIEALGRAHYAYDKGGEEHYNLASALI
                     KSLRNSDVDASLYWLARMIEGGADPVFIARRLCILASEDIGLADPQAMVQAAAAADIV
                     QLIGLPEALFPLSQAAIYLARAPKSNAVKNAYHAAVSDAAETARVPVPLHLRNAVTPL
                     MKHLGYGKDYRYVHSDPNAKEEMECLPEQLRGRTYFNPEEVQPDKNKGPHSE"
     gene            1542..2366
                     /locus_tag="VFO72_07105"
     CDS             1542..2366
                     /locus_tag="VFO72_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9479091.1"
                     /translation="MSSGKTDKELAFLQDLFIAPDWGERFAELIDEHVQLPNEGRALY
                     AAAGTGAHAMSLQERAGSKLALLCVDENSECVELAQAKATATNEATEFRLANLDNLDL
                     PDNRFDLVVGNASLVSRPRVRKMFSEMVRVAAPGATIAIALPTASSFGEFFSIYWEAL
                     HNRDLLDHEPDVEHLITELPTVSDVEQMAADEGLRDIASWTRIEEFDYDSGEQFLSSP
                     LVAEFLMREWLALVPETERADLFREITRLINEERHEAEFALSVKATLVVGQKEHSH"
     gene            complement(2403..2609)
                     /gene="tatA"
                     /locus_tag="VFO72_07110"
     CDS             complement(2403..2609)
                     /gene="tatA"
                     /locus_tag="VFO72_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9479092.1"
                     /translation="MSQTMLAIANLGPTEILLIVLVIFLLFGATRLPQLAKALGQSKK
                     AFREGIEEGEREVREVRKEPEKTA"
     gene            complement(2656..3135)
                     /locus_tag="VFO72_07115"
     CDS             complement(2656..3135)
                     /locus_tag="VFO72_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9479093.1"
                     /translation="MCTERRAIVMIFKGVARVEEHNGHILHSQRLAIHAPSVIRLTEY
                     IHIPFERRSLSRKNILLRDHSTCQYCGRQYPPSELTLDHVQPRSRGGESAWDNLVACC
                     KRCNHKKGNRTPEESGMHLLRRPRGFSLHVNRQIMRYLGRADETWRKYLFYESDNRE"
     gene            complement(3203..>3761)
                     /locus_tag="VFO72_07120"
     CDS             complement(3203..>3761)
                     /locus_tag="VFO72_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9479094.1"
                     /translation="IEVPADVRKEIRCRRCDSQSFTAARAVGSYENALRQSVLSLKRQ
                     PHVSSHLTDLLVAVARREPLAGITRIVPVPLHEKRVRERGFNQAAVIAGVLSKQLELP
                     LDEASLWRIGASEKYRAGLDAKGRRDTVAGAFAVRHPRLVAGENILLVDDVFTTGATV
                     NACAEVLLGAGARQVSVLTVARGKS"
BASE COUNT          808 a         1126 c         1046 g          781 t
ORIGIN      
        1 ctttaatcaa cggcttccgt gccgacgcaa agtatacgca catttcggcg atggcgttcc
       61 catgaagatt cttgcagcct ttgcccggaa aaatggggct ttggctgcga tctgttgacc
      121 cccgggaaaa gtagcggcta agtcatccag ctggtgatcc ggcggtcgct tgttcgattc
      181 ttttcggcga taattataca tccttttggg caccatggac cgctggaatt caaggagtct
      241 gtttggcgat gtgggcgacg ccacgcccga gaccgtcccc gtagagggcg cgcctctggc
      301 cgagcgcatg cgaccgcgtt cgcttgccga attcgtgggt caaacgcatc tggtcggcga
      361 gggccgcatc ctgcgccaac tgctttctcg cggcggctca ctcccgtcgc tgatcctttg
      421 gggtgcgccc ggtacaggca aaaccacgct cgcacgattg ctcgccgaga agagcggcat
      481 gcgcttcatc gcgctgtccg cggtcttctc cggcgtcaaa gacctccgcg aagcgatcca
      541 agaagcaaag acggagcaac gctacggcaa gcgcaccgtg ctttttatcg acgagattca
      601 tcgcttcaac aaatcgcagc aggacgcgct gctacatgct gtcgagaacg gcaccgttac
      661 cctcatcggt gcaacgaccg agaacccctc gtttgaagtt accggcgcac tcttgtctcg
      721 ttgccgtgtg ctcgtcttga atccgctctc tgaagatgaa atcaagactg tgctgcaacg
      781 cgcactcgac gacaacgaac gcggtctcgc aaattatcaa ccatcgatct ccgatacgct
      841 attgcaacga atcgcacata cggcggctgg cgacgcacgc gttgcattga ctgctctgga
      901 cacggccgtc gaatcgaccg cgcccgatgc aaacggcgtc cgcactatct ccgaacagac
      961 ggtcatcgaa gcactgggcc gcgcacacta cgcgtacgat aagggcggcg aggaacacta
     1021 caacctggct tcggcgctga ttaagtcgtt gcgcaattca gacgtcgatg cctcgctcta
     1081 ctggctcgcg agaatgatcg aaggcggcgc ggatccggtg tttatcgccc gccgtctgtg
     1141 cattctggcg tcggaagaca tcggtctcgc ggatccgcag gcgatggtgc aggcggcagc
     1201 cgcagccgac atcgtgcaac taatcggtct tcccgaagca ctgttcccgc tatcgcaggc
     1261 cgcgatttat ctcgcccgcg cgcccaaatc gaacgccgtg aagaatgcct atcacgcggc
     1321 ggtaagtgac gcagccgaga ccgcccgcgt gccagtgcca ttgcacctgc ggaacgcagt
     1381 cacaccgctg atgaaacatc tcggctatgg taaggactat cgctacgtcc acagcgatcc
     1441 gaacgcaaaa gaagaaatgg agtgtttgcc ggagcaactg cgcggcagaa cgtatttcaa
     1501 cccggaagaa gtacaaccag acaagaacaa ggggccccat agtgagtagc ggaaaaactg
     1561 acaaggaact tgcctttctt caagaccttt ttatcgcgcc cgattggggg gagcgctttg
     1621 ccgaactgat cgacgaacac gtgcaactgc caaacgaagg acgcgcgctt tacgccgcgg
     1681 ccggaaccgg cgctcacgca atgtcgctcc aggaacgcgc cggcagcaaa ctcgcgctgc
     1741 tctgcgtcga cgaaaactcc gagtgcgtgg aactggccca agccaaagcc acagccacaa
     1801 atgaagcgac tgagtttcgc ctcgccaacc tcgacaacct cgatctccct gacaatcgtt
     1861 tcgatctcgt cgtcggtaac gcgtcgctag tctcgcgtcc gcgcgttcgc aaaatgtttt
     1921 ccgagatggt gagagttgca gcgcccggcg cgacgatcgc aatcgcactg ccaactgcat
     1981 cgagcttcgg cgagtttttc tcgatctact gggaagcgct gcacaaccgc gatttgctgg
     2041 accacgaacc agacgtcgaa catttgatta ccgagctacc gaccgtctct gacgtcgagc
     2101 agatggccgc agatgaaggc ctgagagaca tcgcgtcatg gacgcgaatc gaggagtttg
     2161 actacgactc aggcgaacag ttcttgagct cgccgctcgt agcggagttt ctgatgcgcg
     2221 aatggctggc ccttgttcct gagacggagc gggcagacct attccgcgaa atcacccgat
     2281 tgatcaatga agaacggcat gaggctgaat tcgcgctttc ggtgaaagcg actttggttg
     2341 tagggcagaa ggaacacagt cactgaactg gggttagtag ctaagccgtc cgccaacagc
     2401 cgctacgcgg tcttctccgg ctctttccgt acttcccgca cttctctttc gccttcttcg
     2461 atgccttctc tgaatgcctt cttgctctga cccagggctt tcgctagctg gggcaggcgg
     2521 gtagccccga agagcagaaa gatgaccagt acgattaaga gaatttctgt cggtccgagg
     2581 tttgcgattg cgagcattgt ttgactcatt gtgtcctctt ctttcagatg gtcgttgcta
     2641 cggccactga gctaattatt cccgattgtc cgattcgtaa aagagatact ttctccacgt
     2701 ctcgtcggca cggccgaggt accgcatgat ctggcggtta acgtgtaacg agaacccgcg
     2761 aggtctgcgc agcagatgca tgcccgactc ttcaggagtc cggttacctt tcttgtggtt
     2821 acaacgcttg cagcaggcaa ccagattatc ccatgcagac tcccctccgc gcgagcgcgg
     2881 ctgcacatgg tcaagcgtga gttctgaagg cggatactgc ctaccgcagt actggcaggt
     2941 cgagtggtca cgcagcagga tgttctttcg cgagagcgag cgccgctcga aaggaatgtg
     3001 aatgtattcg gtcaggcgaa tcaccgacgg cgcatgaatt gcgagtcttt gcgaatgaag
     3061 tatgtggccg ttgtgttctt ccacgcgagc tacgcccttg aagatcatca cgatagcgcg
     3121 cctctcggtg cacacgttga ttggctcaaa agacgtatta aggactaaaa cccggccatt
     3181 cataactgcg tgatattacg cgctaactct tccctcgcgc aacggttaac accgaaacct
     3241 gcctggcgcc cgcccccaaa agcacttcgg cgcaggcgtt aacagtcgct ccggtggtaa
     3301 aaacatcgtc taccagcagg atattctcgc ctgcgaccag ccgcggatgg cggacggcaa
     3361 aagccccggc gacggtgtcg cgccggccct ttgcgtcgag tccggcccgg tacttttccg
     3421 acgctccaat ccgccacaag cttgcttcgt ccagcggcaa ttcgagttgt ttcgaaagaa
     3481 cgccggcaat cacggctgcc tggttgaacc ctctctcgcg gactcgtttc tcatgtaagg
     3541 gcaccggaac gattcgcgta atgccggcta acggttcgcg tcgcgcaacc gcgaccagca
     3601 ggtcagtcag atgggacgaa acgtgcggtt ggcgcttcag tgaaagcacg gattgacgca
     3661 acgcgttctc atacgagccc actgcgcgag cggcagtaaa cgactgtgag tcacaacgcc
     3721 ggcacctgat ctctttcctc acgtccgccg gcacctcgat a
//
LOCUS       DASTEY010000401         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1393094, whole genome shotgun sequence.
ACCESSION   DASTEY010000401 DASTEY010000000
VERSION     DASTEY010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393094"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFO72_07125"
     CDS             complement(<1..1187)
                     /locus_tag="VFO72_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479095.1"
                     /translation="MKCLYLSLLLAINITVVTAQEPEVVRINTDLVQTAVTVLDKKGN
                     FVDGLQRDQFELIVDGKPRQIAFFERVAAGSTRERELASRSLVVNSTAPLPPATVPGR
                     TIVFFIDDLHLSPDSMNRTRMMLQHFLEREMNSKDNVAITSASGQIGFLEQFTNNRAV
                     LDAAVGRLVPRPYDAHGYSAGNATKMTEYMALNIDTTKSDTKVLDFYVTECMKGAATF
                     KKARQALALIRASCETQVKNSARAILMQAAQITQNTYNSLESLMRSAARAPGRKLAFF
                     VSDGFLLDAGPHAATTRDKLDHVIDAAQRGGVVVYTIHARGLVNSSFLDPGNNRPLDP
                     AGRIDVASIGELEAHQDALSALAAQTGGRALRSTNFFDRWVSTVLDETSNYYLVAWRP
                     END"
     gene            1226..2635
                     /gene="fumC"
                     /locus_tag="VFO72_07130"
     CDS             1226..2635
                     /gene="fumC"
                     /locus_tag="VFO72_07130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET9479096.1"
                     /translation="MSQSSPEQTAKTRTETDSMGAIEVAADRYWGAQTERSLHHFNIG
                     DDRMPREMVRALGILKKAAAQVNEELGKLPADKARLIIQACDEVIEGKLDSHFPLRVW
                     QTGSGTQTNMNANEVISNRAIELAGGVMGSKKPIHPNDDVNMSQSSNDTFPTAMYIAA
                     AEQLNKLVPRVQELHDAIAAKAKEFAGVVKIGRTHLQDATPLTVDQEMGGWASLIERD
                     LERLQTAMPGLLDLAIGGTAVGTGLNSHPEFGERAAKKIAELTGLPFRSHPNKFAALS
                     AHDELVFASGALKTLAASLMKIANDVRWLASGPRCGLGELILPENEPGSSIMPGKVNP
                     TQSEALTMVAAQVFGNDLATSFGGSQGNFELNVYKPVMIYNFLHSVRLLHDAIGSFVE
                     FCISGIELNREQIDTHLKASLMLVTALNPHIGYDKAAQVAKNAHKKKISLRQSAIELG
                     FLTGEEFDRLVQPQEMTHP"
     gene            2666..2908
                     /locus_tag="VFO72_07135"
     CDS             2666..2908
                     /locus_tag="VFO72_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET9479097.1"
                     /translation="MPDKNEKVKENVNGSFYVDSSCIDCDVCRDTAPENFMRSDENNY
                     SFVYKQPENPEEQAMCEEAMSCCPVEAIGNDGSEAV"
     gene            complement(3067..3435)
                     /locus_tag="VFO72_07140"
     CDS             complement(3067..3435)
                     /locus_tag="VFO72_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET9479098.1"
                     /translation="MQSFNTETDDTPPAENANQTLIVGATGDLRPGACLRVELPDGDE
                     LAVYNVGGEYYATENFCPHKGAPLSEGALCGHIIECDWHGWQFDVRSGQCLTVPEKIK
                     TYDVFVEDGLVKVIQRTDAL"
BASE COUNT          826 a          974 c         1025 g          762 t
ORIGIN      
        1 tcatcgtttt caggacgcca ggctacgaga tagtaattcg aagtctcgtc aagaaccgtg
       61 ctgacccacc tatcgaagaa gtttgtgctg cgcagggcgc gtccaccggt ctgggccgca
      121 agggcactga gtgcatcttg atgagcttcg agttcgccga tcgaggcgac gtcaatgcgg
      181 ccggcgggat cgagcggacg attgttgcct ggatcgagaa agctgctgtt gaccaagcct
      241 cgcgcgtgaa tcgtgtagac gactacacct cctcgctgcg cagcgtcgat aacgtggtcc
      301 agcttgtcgc gcgtggtggc cgcgtgtggt ccagcatcga gcagaaaacc gtcggagaca
      361 aagaacgcca gcttacgccc tggcgcgcgg gccgctgaac gcatcaacga ctcgagcgag
      421 ttataagtgt tctgcgtgat ctgcgcagcc tgcatgagaa tcgcgcgagc actgtttttg
      481 acctgcgttt cacacgacgc acgaatgagc gccaacgcct gtctggcctt cttaaacgtg
      541 gcagcacctt tcatgcattc tgtaacgtag aagtcgagca ctttggtatc ggacttagtc
      601 gtgtcgatgt tgagggccat gtactccgtc atcttcgtag cgttgccggc gctgtaaccg
      661 tgtgcatcgt aggggcgcgg aaccaagcga ccgaccgccg catcgagcac ggctctgttg
      721 ttcgtaaact gctcgagaaa accgatctgg ccactggctg aagtaatcgc gacgttgtct
      781 tttgaattca tctcacgttc gagaaagtgt tgcagcatca tgcgtgtgcg attcatgctg
      841 tcaggcgaga gatgaagatc gtcgatgaaa aagacgatcg tgcgtcccgg gaccgtcgcg
      901 ggtggcagtg gcgccgtgga attgacaacg agacttcgac tggcgagttc gcgctcgcgt
      961 gtgctgccgg cggctacacg ctcgaaaaat gcgatctgac gcggtttgcc gtcgacgatt
     1021 aactcaaact gatcgcgctg tagcccatcg acaaagtttc ccttcttgtc gaggactgtc
     1081 acggcagtct gcaccaggtc agtattgatg cggacgactt ccggttcctg cgcggtaaca
     1141 acagtgatgt tgatcgcgag tagaagagat agataaaggc acttcatggg attctccgcg
     1201 cgggaatgat attattaacc cgccaatgtc tcaatcatcc cctgaacaaa cagccaaaac
     1261 tcgaactgaa accgactcga tgggagccat cgaggtggcc gccgaccgct actggggcgc
     1321 acagaccgaa cgctcgctcc accacttcaa tatcggcgac gatcgtatgc cgcgcgagat
     1381 ggtccgcgcg cttggcattc tcaaaaaagc agccgcacag gtaaacgagg aactcggaaa
     1441 gctgccggcc gacaaagccc gtctgatcat tcaggcctgt gacgaagtca tcgaaggcaa
     1501 actcgatagc cactttccgt tgcgcgtgtg gcagacaggc agtggcacgc aaaccaacat
     1561 gaacgccaac gaggtgatct caaaccgcgc gatcgaactc gccggcggcg tgatgggcag
     1621 caagaaaccg atccacccaa acgacgacgt caacatgtcg caatcgtcga acgacacgtt
     1681 tccgactgcg atgtacattg ccgccgccga acagttgaac aagctggttc cgcgcgtgca
     1741 ggaactgcac gacgccatcg ccgccaaagc gaaagagttt gcgggcgtcg tgaaaatcgg
     1801 acgtacgcat cttcaggacg ccacaccgtt aacggtggat caggagatgg gcggctgggc
     1861 gagcctgatt gaaagagatc tcgagcgcct gcaaactgcg atgcccggtc tgctcgacct
     1921 cgcaattggc ggcactgccg tcggcaccgg acttaactca catcccgaat ttggcgagcg
     1981 cgctgcgaag aagatcgcgg agttgacggg tctgcctttc cggtcacacc cgaacaagtt
     2041 cgcggcgttg tcggcgcacg acgaactggt tttcgccagt ggcgcattga aaaccctcgc
     2101 ggcgtcgttg atgaagattg cgaacgacgt gcgctggctc gcttccggtc cgcgctgcgg
     2161 tttgggtgag ttgatacttc ctgagaacga gcccggttcg tcaatcatgc ccggcaaagt
     2221 gaatccgacg cagagcgaag ctttgacgat ggtggccgcg caggtctttg gaaacgatct
     2281 cgctaccagt ttcggcggct cgcaaggcaa tttcgagctg aacgtttaca agccggtaat
     2341 gatttacaac tttcttcact cagtaaggtt gctgcacgat gccatcggga gttttgtgga
     2401 gttctgcatc tcgggcatcg agcttaaccg ggaacagatc gatacgcatt taaaagcctc
     2461 gttgatgctg gtcacagcgt tgaaccctca cattggttac gacaaagccg cccaggtagc
     2521 aaaaaacgct cacaagaaga agataagcct gcgacagtct gctatcgagc tcggattttt
     2581 gacaggcgaa gagtttgacc ggctggttca gccgcaagag atgacgcacc cgtaacgcag
     2641 ttgcgcggat cttcagtttc gaaagttgcc tgacaagaac gaaaaagtta aagagaacgt
     2701 caacggctcg ttttacgtgg actcctcgtg tatcgactgc gacgtctgtc gcgacaccgc
     2761 gccggaaaat ttcatgcgct ccgatgaaaa caactactcc tttgtctata agcagccgga
     2821 aaatcctgaa gagcaggcga tgtgtgaaga ggcgatgagt tgctgcccgg tcgaagctat
     2881 cgggaacgat ggtagtgaag cagtttgaaa ttgggatcgg ggatggcttt gcgtttgggg
     2941 gataaacgtc acttccacgc cggtcgtgct cttccctggg accgcaggcg tctcgcctgc
     3001 tggattcggc agtggccttc tgcgctgcaa gcaggcaaga cgcctgcggt cccagcaacg
     3061 aggtgttcaa agtgcatcag tacgctgaat gactttcact aaaccgtctt caacaaacac
     3121 gtcgtaggtc tttattttct cgggcacagt cagacactga ccactccgga cgtcaaactg
     3181 ccagccgtgc caatcacact caataatgtg cccacataat gcgccttcag acagcggcgc
     3241 tcccttgtgc ggacagaaat tctcggtggc atagtattcg ccacctacgt tgtaaacagc
     3301 taactcgtcg ccgtcgggaa gctctactct gaggcaggcg ccgggtcgca ggtcgccggt
     3361 tgcgcccaca atcaaagttt gattggcgtt ttcggctggt ggggtgtcgt ctgtttcagt
     3421 attgaatgat tgcattgaat ggatggtaac aaaacacgca agatcttgct tccggcgcgt
     3481 tctctacgtc cgcggccaat taatctctcc aaagccgtga gatcgagcaa acggaaggtg
     3541 agattatgaa acgactactg accataaccc aactgatggc gctcgtg
//
LOCUS       DASTEY010000402         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_58291, whole genome shotgun sequence.
ACCESSION   DASTEY010000402 DASTEY010000000
VERSION     DASTEY010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58291"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VFO72_07145"
     CDS             complement(<1..949)
                     /locus_tag="VFO72_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479099.1"
                     /translation="MTFEPRRKLRIIVLVHEDLVPPDSLNGLSDKEKLEIKTEFDVTS
                     TLKKMGHEVYPVGLYNQLNVIGNALMEHKPHIAFNLLEEFHGYPLYDQHVVSYLELMK
                     QAYTGCNPRGLTLAHDKALTKMILSYHRLLVPNFAVFPMNRKPKRPKRLKFPLLVKSI
                     SEEGSVGIAQASVVHDDEKLAQRVEFIHRQNKTAAIVEQYIKGREIYVGVIGNQNIQT
                     YAPWELVMEKLPEGAENIATLKVKWDPAYQERVGVKTRAADLTPEQHKTLERLSKRIY
                     RYMFLSGYARLDYRMTDEGQFYLLEANPNPQIAHNEDFAD"
     gene            complement(946..1965)
                     /locus_tag="VFO72_07150"
     CDS             complement(946..1965)
                     /locus_tag="VFO72_07150"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET9479100.1"
                     /translation="MAYRFPWQRLTDEQLLDLRICDLPIKIKGSFLEPHIKRLYSELN
                     SRGIRFKPHVWLAEEWFSPDGVPGIAIPFYLAHPRLIKLERRQMLEVEGGTDTECMRI
                     LRHEAGHALDTAFRLHFKRQHRELFGSFAQPYPDFYKPRPKSRRYVLHLPAWYAQAHP
                     AEDFAETFAVWLTPRSQWRRRYRGWPALQKLEYIDELMDQIAGTKPRNRTRTKVEPLS
                     ALKITLREHYRRKRERYDFQWPNVYDRELRRIFSDDPRHKTKPSAASFLRKVRREVRQ
                     IVAEGTGVHQYTIDHVLENMIDRCKELGLRVTTPFRETRRRVMIVLTVQVMNVLHSGY
                     HRIAV"
BASE COUNT          435 a          585 c          574 g          520 t
ORIGIN      
        1 agtcggcgaa gtcttcgttg tgcgcgatct gaggattcgg attcgcctcc aacaaataga
       61 actgtccctc gtccgtcatg cggtaatcga gccgcgcata accactcaag aacatataac
      121 gatagattcg ttttgacagt cgctcgagcg tcttgtgctg ttcgggtgtg aggtctgcgg
      181 cccgcgtctt cacgccgact ctttcctggt aggccggatc ccatttcacc ttcagcgtcg
      241 cgatgttctc cgcgccttcg ggcagttttt ccatcaccag ttcccacggc gcgtaggtct
      301 gaatattctg attgccaatc acgcccacgt agatttcgcg gcctttgatg tactgctcaa
      361 ctatagccgc cgtcttgttt tgccggtgaa tgaactcgac acgctgcgcc agcttttcat
      421 catcgtggac cactgacgcc tgcgctattc caaccgaacc ttcctcgctg atcgacttca
      481 ccaacagcgg aaacttcaaa cgtttcgggc gcttcggttt tcggttcatg ggaaacacgg
      541 cgaagttcgg caccagcagg cggtgatacg acaggatcat ctttgtcagc gccttgtcgt
      601 gcgctaacgt caggccacgc gggttgcagc ccgtgtacgc ctgcttcatc agttcgagat
      661 aactgacgac gtgctggtcg tagagcggat aaccatgaaa ctcttccagc aggttgaacg
      721 cgatgtgagg cttgtgctcc atcaacgcat tgccgattac gttgagctgg ttgtacaagc
      781 cgaccggata gacctcatgg cccatcttct tcaacgtcga agtcacgtca aactcggttt
      841 tgatttccag cttttctttg tcggagagac cgttcaacga atcgggaggc acgaggtctt
      901 cgtgaacgag cacgatgatg cgcagtttgc gtcgtggctc aaaggtcata cggctatgcg
      961 atgataacct gaatggagca cgttcattac ttgcacagtc agcactatca ttacacgtcg
     1021 acgcgtttcg cgaaagggtg ttgttactcg tagtcctaat tctttacagc gatcgatcat
     1081 attttccagc acgtgatcga tggtgtactg gtgcacgccg gtgccttcgg cgacgatctg
     1141 tcgtacttca cggcgcacct tccgaagaaa actcgcggcg ctcggctttg tcttgtgacg
     1201 cggatcgtca gaaaaaatcc ggcgcaactc gcgatcgtaa acgttgggcc attggaaatc
     1261 gtaacgttca cgcttgcggc ggtagtgttc gcgcaaggtg atcttcaacg ctgaaagtgg
     1321 ttcgacttta gtgcgtgtgc gatttcgcgg tttcgtgccg gcgatctggt ccatcaactc
     1381 gtcgatgtac tcgagcttct gcaacgcggg ccagccgcga taacggcgac gccattgcga
     1441 acgaggggtc agccacacag cgaaggtctc ggcgaagtct tccgccggat gcgcctgcgc
     1501 gtaccacgcc ggcaagtgca agacgtatct acggctcttc ggccggggtt tgtagaagtc
     1561 gggatacggc tgcgcaaacg agccgaacag ttcgcggtgc tggcgcttga agtgcagtcg
     1621 aaatgccgta tcgagcgcgt ggcccgcttc gtgccgcagg atgcgcatgc attcggtgtc
     1681 tgttccgcct tcgacttcca gcatctgccg tctttcgagc ttaatcaggc gcgggtgggc
     1741 tagataaaac gggatcgcga tacccggcac gccgtccggt gaaaaccact cttcagccag
     1801 ccaaacatgg ggcttaaagc gaatgccgcg ggagttcagt tcgctgtaaa gcctcttgat
     1861 atgaggttcg agaaatgagc ccttgatttt gataggcaag tcgcagatgc gcaggtcaag
     1921 caactgctcg tccgtgagac gttgccaggg gaatctgtaa gccacggcgg cacattctag
     1981 ccgcctcgcc taatcggtac aaaccgtgct acttttgcct tctacctttc gcgtttcata
     2041 tcgagatcaa accgggagta ccaatgctaa aaacaatact ttctatcatc tgtctcttcg
     2101 tcattgcagg cgct
//
LOCUS       DASTEY010000403         3043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_58292, whole genome shotgun sequence.
ACCESSION   DASTEY010000403 DASTEY010000000
VERSION     DASTEY010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58292"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFO72_07155"
     CDS             complement(<1..189)
                     /locus_tag="VFO72_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9479101.1"
                     /translation="MQDRQFVHLHLHTDYSLLDGAIQIKPLSSRVAELGMPACAMTDH
                     GNMFGAISFYNAMKNNGVK"
     gene            complement(238..1794)
                     /gene="guaA"
                     /locus_tag="VFO72_07160"
     CDS             complement(238..1794)
                     /gene="guaA"
                     /locus_tag="VFO72_07160"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9479102.1"
                     /translation="MNQVRQHETVIILDFGSQYTQLIARRVREAGVYCEILPFNTPIE
                     TIRSRSPKGLIFSGGPSSVYDEGAPKPQPELLESVDCPALGICYGFQVFARELGGAVE
                     SSPNREFGYAGLKVLDVTSRLFRGLPEEMDVWMSHGDHVTKVPPGFHVTALTDDALNA
                     MEDESRGIYGVQFHPEVAHTPLGAHVLRNFLFSVCGCRGDWSPEAVIEEQVAKIRVHL
                     GEDGRAVSGLSGGVDSTVAAALVHRAVGDRQTCIFVDNGLLREGEFESTLALLRQSMK
                     LNINGVRAGKLFLEALRGVTDPEQKRKRIGKVFIEVFEEEARRIGEVGYLVQGTLYPD
                     VIESVSVRGPSAVIKSHHNVGGLPEMMKLKLVEPLRELFKDEVRLIGRELGVPEEILG
                     RHPFPGPGLAVRIIGEVTEEKIRLVQAADRILDEELRLAELYNAVWQAFPVLLPVSTV
                     GVMGDERTYERVIAIRAVTSVDGMTADWARLPHDLLGRVAGRIVSEIRGVNRVVYDIS
                     SKPPSTIEWE"
     gene            1803..2900
                     /locus_tag="VFO72_07165"
     CDS             1803..2900
                     /locus_tag="VFO72_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9479103.1"
                     /translation="MKLRIIHLLCVVSLAWGSVFTAEKADKEPLLSQPLTIRWRYESN
                     VTLNLTPAFDKERIYLPLAGGTIVALNARDGQLYWRSDMGGELSASPVADESAIYVAS
                     ETTAPNREPRNSSGALRALGREGGVTQWMTPLVKPLRGALSISDKKLFAGGSDGRAYA
                     FDKRTGGVLWSIPFPSPFNGQPVLNSGRVYFGSEDGTLLALEESTGRLLWRFRTKGAI
                     HGPVAATDTAVFFGSGDGYVYAVSADKGRLKWRKRTGAGVEAVALAGDALLAASLDNF
                     AYLLNLKGTMLWKKQLPGRISAQPLTVQETALFTPLSSSAAVLLQLRDGKQVNTLPTG
                     EELTSSASPIMVGDAVIVTTEHGLLAFGRPS"
BASE COUNT          688 a          917 c          805 g          633 t
ORIGIN      
        1 cttcacgccg ttgtttttca tcgcgttgta aaaagagatc gcgccgaaca tgtttccatg
       61 atcggtcatg gcacacgcgg gcattcccaa ctcggcaacg cgactcgaga gcggctttat
      121 ttgtatggcg ccgtcaagaa gactgtagtc agtatgaaga tgaagatgaa cgaattgacg
      181 atcctgcatc ggttgtaaac ccgtccacag actacgcaaa aactctgtgg atggccttta
      241 ttcccactct atagttgaag gtggcttgga actaatatcg taaacgactc gattaacacc
      301 gcgtatctct gaaactatcc ggcctgctac acgccccagt agatcgtgcg gcaagcgcgc
      361 ccagtccgcc gtcatgccgt cgacgctcgt gaccgcacgg atcgcgatga cgcgttcgta
      421 cgtgcgctcg tcgcccatta cgccgacagt agagacgggc aacagtaccg gaaaagcttg
      481 ccagaccgcg ttgtataact ccgccaatcg caactcttca tcgagaatgc gatccgccgc
      541 ctggaccaac cggatcttct cttccgtcac ttcgccaatg atacggacgg ccagtcccgg
      601 accgggaaac ggatgccgtc cgaggatctc ttcaggcacg ccaagttccc tgccaatcag
      661 acgcacttca tctttgaaca actcccgcag cggctcgacc agcttaagct tcatcatctc
      721 cggcaggcca cccacattgt gatgactctt aatcaccgcc gacggcccac gcaccgaaac
      781 cgactcgatc acgtccggat acaaagtgcc ctgcaccaga tagcccactt cgccgatgcg
      841 ccgcgcttct tcttcgaaga cctcgatgaa cactttgccg atccgctttc gtttctgttc
      901 gggatcggtt acgcctcgca acgcttcgag aaacaacttg cccgcgcgaa caccgttgat
      961 gttcaacttc atgctctgcc gcagcagcgc gagtgtcgat tcaaattcac cttcacgcag
     1021 cagaccgtta tcgacaaaaa tgcaggtctg ccgatcgccg accgcgcgat gcaccagcgc
     1081 tgccgcaacc gtcgaatcga cgccacctga caaacccgaa acagcgcggc cgtcctcgcc
     1141 caagtgcacg cgaatctttg cgacttgctc ttcaataact gcttcaggcg accaatcgcc
     1201 gcggcagcca caaacagaga acagaaaatt ccgcaacacg tgcgcgccta acggcgtgtg
     1261 cgccacttca ggatgaaact gcacgccgta gatgccgcgc gattcatctt ccatcgcgtt
     1321 cagcgcgtcg tcggtcaggg ctgtaacgtg aaaaccgggt ggcactttcg tcacgtgatc
     1381 gccgtgactc atccagacgt ccatctcctc gggcagtccg cgaaacagac ggctcgtcac
     1441 gtccaggacc ttcagccctg cataaccaaa ctcccgattc ggggacgact cgaccgcgcc
     1501 gccgagttca cgcgcaaaca cctggaagcc gtaacaaatc ccgagtgccg gacaatcgac
     1561 ggattcgagc aactccggct ggggtttggg cgcgccttcg tcgtagacag acgacggccc
     1621 gccggaaaag atcaatcctt ttggcgagcg cgagcgtatt gtctcgatcg gagtgttgaa
     1681 cggaaggatc tcacagtaaa cgcctgcttc tcgaacgcgc cgggcgatca gctgtgtgta
     1741 ctgtgatccg aagtcaagta tgattacggt ttcatgctgc cggacttggt tcattgattg
     1801 atatgaagtt gcgaattata catctactct gtgtcgtcag tctcgcttgg gggagtgttt
     1861 ttaccgcgga gaaggcggac aaagaaccac tattgtcgca gccgctcact atccgatggc
     1921 gttatgaatc aaacgtcaca ctaaatctca caccggcttt cgacaaagaa cgcatttatc
     1981 tgccactcgc cggcggcact atcgtggcat taaacgctcg cgacgggcag ttgtactggc
     2041 gctctgacat gggcggagaa ctttcggctt cacccgttgc agatgaatca gcaatttacg
     2101 tcgcctcgga aacgaccgcg ccgaaccgcg agccgcgcaa ctcgtccggc gccttacgcg
     2161 cgctcggacg cgaaggcggc gtgacgcagt ggatgactcc gctggtcaag cctctgcgcg
     2221 gagcactgtc aatcagcgac aaaaaactgt tcgccggcgg tagtgatgga cgtgcatatg
     2281 ccttcgacaa acgcactggc ggcgtgttgt ggtcgattcc ctttccctcg cctttcaatg
     2341 gacaaccggt gctgaattcg ggacgcgtct attttggcag cgaagacggc actctcctgg
     2401 cgcttgaaga atccaccggc cgcctgcttt ggcgctttcg cacgaaaggc gcgatccatg
     2461 gaccagtagc agcaactgat acggctgtgt tcttcggctc gggtgacgga tacgtctacg
     2521 ccgttagcgc cgacaaggga cgtttgaagt ggcgcaaacg aacgggcgcg ggtgttgaag
     2581 cagtagcgct tgccggcgac gccttactcg cagcttcgct cgacaatttc gcctatctcc
     2641 tgaatctgaa aggcacgatg ctctggaaga agcagttgcc gggacgaatt tcggcgcaac
     2701 cattaacggt tcaggagaca gcgctcttta caccactctc gagttccgcg gcggtattgt
     2761 tgcagctaag agatggaaaa caagtgaata ctctaccgac cggtgaggag ctaacgagtt
     2821 cagcatcgcc catcatggtc ggcgacgctg tcatcgtaac cacagagcac ggactgttgg
     2881 cctttggccg cccgagttaa aaaatcattt gtgctcaggg acccgaagaa cgaccgacag
     2941 ggtttgcttc gatcttggct tcagttgatt caccgcgtcg ccagaagaca ccggcaaccc
     3001 gggagatgag gccatgcagg atatagctga tcacgatcgc caa
//
LOCUS       DASTEY010000404         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_733834, whole genome shotgun sequence.
ACCESSION   DASTEY010000404 DASTEY010000000
VERSION     DASTEY010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733834"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFO72_07170"
     CDS             <1..344
                     /locus_tag="VFO72_07170"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET9479104.1"
                     /translation="ATAMVSALSTRPVRKDVAMTGEITLRGNVLPIGGVKEKVLAARR
                     ARVTTIILPQQNRRDLDEVPKEPFKDVRFVFVDNMRQVFREALGEKVIPPVSSGARPQ
                     RMPQAASPPPA"
     gene            420..1010
                     /locus_tag="VFO72_07175"
     CDS             420..1010
                     /locus_tag="VFO72_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET9479105.1"
                     /translation="MSTTRCSYPSILFAVLTFVFASSITAVAQQEELKIKNFGQMDDR
                     FYRGGQPKEEDYRHLAAIGIKTIIDLRNDPKEYEKREAEEFGMRYINIPMSDKAYPQT
                     SAIDQFLKLVDDPSTGKFFVHCAGGRHRTGVIGAVYRFNHYNWNYEQVYAEMKKYDFY
                     TRWGHGNMKKFVKDYALTFENRAATAAAAVTTTINQ"
     gene            complement(1115..2032)
                     /locus_tag="VFO72_07180"
     CDS             complement(1115..2032)
                     /locus_tag="VFO72_07180"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HET9479106.1"
                     /translation="MKQQRRREVITLAVVFTGVLLLSALAVYFIGSRGTPEFSQVQQP
                     AAADREPVSRPSPTPTPADNETFLDHLMGTRHPEAYVRGEPWIASVGRIALRLLLAAL
                     LGAALAYRPRRRILALKPNPSVSQTQILLAIVAAALMIIVGDNAARAFGIFAAVSLVR
                     FRTNIRDPKEVTVLLICMALGLASGVGRWDLGLVLAVFSLLALWALEWREPEQVFRSM
                     ELKVITRDVVSTQRALREMFRKHGFDKELRSVDREATEESPGSIMYSVDVSPTVSTDE
                     LSADILALDGQNVEGIEWSQKKSYSYLYQ"
     gene            complement(2029..>2382)
                     /locus_tag="VFO72_07185"
     CDS             complement(2029..>2382)
                     /locus_tag="VFO72_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479107.1"
                     /translation="LGATFGHASTTPLFWRITRSRNNPKNYAKSEFLEKVKGNRVVVH
                     FGGKNRGLMKNITVDDAIWMGTWLAQLSDKQVRDAFRAANYSPHQVDLLARTVRVRTS
                     ELLSLRPNVHIGSTQ"
BASE COUNT          572 a          667 c          636 g          507 t
ORIGIN      
        1 tggcaaccgc gatggtgtcg gcgttgtcca cgcgaccggt gcgcaaagac gtggcgatga
       61 ctggtgaaat aacacttcgt ggcaatgtgt tgccaatcgg cggcgtgaaa gaaaaagtac
      121 tggctgcccg gcgcgctcgc gttaccacga taattctgcc gcaacagaac cgcagggacc
      181 ttgacgaggt gccgaaggag cctttcaagg acgttcggtt tgtttttgtc gataatatgc
      241 ggcaggtttt tcgcgaggca ctgggcgaaa aagtcattcc gccggtgtct tcgggcgcgc
      301 ggccgcagcg aatgcctcag gctgctagtc ccccaccggc gtgaaatgtg atacaaatac
      361 acgcgacgaa tcccctatcg caagcttagt ccgccagcat tcaagttaaa ggagtttcga
      421 tgagtactac gaggtgcagc tatccttcta ttctctttgc cgttctgacc ttcgttttcg
      481 catcatcaat taccgcagtt gcccagcagg aagagctaaa aattaagaac ttcggacaga
      541 tggacgaccg cttctatcga ggcggtcaac cgaaagagga ggattacaga cacctggctg
      601 cgattggcat taagaccatt atcgatctgc gcaacgatcc gaaagagtat gaaaagcgtg
      661 aggccgaaga gttcggaatg cgctacataa atattccgat gagcgacaag gcctatcccc
      721 aaacttctgc gatcgatcag tttctgaaac tcgtcgacga tccatcaacc ggcaagttct
      781 tcgttcattg cgccggcgga cgtcaccgca ctggcgtgat cggcgccgtc taccgcttca
      841 atcattacaa ttggaactac gagcaggttt acgccgagat gaagaagtat gacttctaca
      901 ctcgctgggg acacggcaac atgaagaaat ttgtgaagga ctacgccctc acctttgaga
      961 atcgcgcagc aaccgctgct gcagccgtca ctacgactat aaatcaatag acgagctttg
     1021 tactttgagc tttgttttct tgctcgtcga caccagattc aaagaagaaa gaacaaagaa
     1081 caaagtacaa agctcaaagc tcaaagcacc accttcactg atacaaatac gagtagctct
     1141 tcttctgact ccactcaatg ccttcgacat tctggccatc aagcgccagg atgtcggcac
     1201 tgagttcatc agtactcacc gtcggactca cgtcaactga atacataata cttcccggtg
     1261 actcttccgt agcttcgcga tcgactgaac gcagctcctt gtcgaatccg tgtttcctaa
     1321 acatctcgcg cagcgcacgc tgcgtcgaaa cgacgtctct ggtaattact ttcaactcca
     1381 tcgaacgaaa cacctgttcc ggttcgcgcc actcgagtgc ccaaagcgcc agcaatgaaa
     1441 acaccgccag cacgaggccg aggtcccaac gaccgacacc ggacgcgagt cccagtgcca
     1501 tacagattag taacacagtg acttccttgg gatcgcgaat gtttgtgcga aagcgaacca
     1561 gcgaaacggc cgcgaagatg ccaaaggcgc gcgccgcgtt atcgccgacg atgatcatca
     1621 gcgccgccgc gacaatcgcc agcagaatct gtgtttgcga aacggaagga ttgggtttga
     1681 gagcgagaat ccggcggcgt ggacgataag cgagtgcggc gccaagcagt gccgcgagca
     1741 gaagtcgcag agcgatccgg ccaacactcg cgatccacgg ctcgccgcga acgtaagcct
     1801 cggggtggcg ggtgcccatg aggtggtcca gaaatgtttc gttgtctgcc ggagttggcg
     1861 tcggtgacgg ccgcgagact ggttcgcgat ctgccgcggc gggctgttgg acctgactga
     1921 attcgggcgt tccgcggctg ccgatgaaat agacagccag cgcgcttagt aaaagcacgc
     1981 cggtaaacac caccgccagg gtaatcactt cgcgtcttcg ttgctgcttc attgagtgct
     2041 accgatgtga acgttcggac gcaggctgag taactcgctg gtccgcactc gcaccgtgcg
     2101 agccaatagg tcaacctgat ggggactgta gttcgcggcg cgaaacgcat ccctcacctg
     2161 cttatcgctg agctgggcca accaggtgcc catccagatc gcgtcgtcga cagtgatgtt
     2221 cttcatcagt ccgcgattct taccgccgaa gtgcaccacc actcgattcc ctttaacttt
     2281 ttcgagaaat tcagacttcg catagttctt tgggttattg cgactgcgcg tgatgcgcca
     2341 gaacagcggc gttgtgctcg cgtgaccaaa cgtcgcaccg ag
//
LOCUS       DASTEY010000405         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1244952, whole genome shotgun sequence.
ACCESSION   DASTEY010000405 DASTEY010000000
VERSION     DASTEY010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244952"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFO72_07190"
     CDS             complement(<1..529)
                     /locus_tag="VFO72_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479108.1"
                     /translation="MFWTCILILLVSFTTVVAQPAAEPTQDLDTLRAEGYEALYNLDY
                     EGARRRFQKMIDLAPDHPAGPQAFAASLWIQQLNESWELKSTLYSDKAYKNEKPKVDR
                     RQVDEFRRWTRRAKQLSEARLRRNARDVEALYFLGAAEGLEAAYSAAVERKFMSALRT
                     GSSAVGHHRKVLELSP"
     gene            643..1449
                     /locus_tag="VFO72_07195"
     CDS             643..1449
                     /locus_tag="VFO72_07195"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9479109.1"
                     /translation="MIAQRRQIFHLSRALICVICALSIGSLLVPIANKFHLALDILLR
                     TYLIFLGTVMAHESVHGHLGSTRRANFWWGRIALLPSMVPFTNFRRTHQLHHAYTNIP
                     DKDPDHFMKSRNLLELSLRAIAMPHHWFFWLRKRGRLKRADLWELFLNYLAIVALYAT
                     VLSFVGPTRLLWGMAPPLILVSLLLWYPFAVQTHEGFSTGSAEARSHDYYGNFMYWFS
                     LGLSMHRQHHLQPNLSWIELRRYVQPAPRRRWWWPQRDIRIDVQQSSDAV"
     gene            1439..>2458
                     /locus_tag="VFO72_07200"
     CDS             1439..>2458
                     /locus_tag="VFO72_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479110.1"
                     /translation="MQFETTDRLPGTAGVSPASHGGAQLVAMQARHLRSQQNGASVFK
                     LPLSVLDYAFSLLDSADSPQDFTIILHLGNPPSLENLRAGSRSATNRYPISGCTIKNR
                     SWVFGGNEPVQLPCDGNGNRSLESFVNERFDVREEVPVRQVLFANNEAERCLVTRFHH
                     AAADGLSAALWLGHQLSVAYELIAPETACARPVEPPLRESDSSVRRSLFAYSGASDPL
                     WTTNYIPSGARRWLTINFPANDLRKGCRRARGFTYNDLLATCVLEVLSNWNRKNDPKA
                     TQKIGLWYPLNIRANSNPNFGNATSRIRLYAKFPAGASLIDKAREVRKQVSWSTSNGE
                     WVLPQL"
BASE COUNT          507 a          722 c          657 g          572 t
ORIGIN      
        1 gaggcgagag ctcgagtacc tttcgatgat ggcccaccgc gctcgaaccg gtgcgcaagg
       61 cagacatgaa cttccgttcg accgcagccg aatacgcagc ttcgagtcct tccgccgcgc
      121 cgaggaagta gagtgcttcg acgtcgcgag cgttgcggcg cagtcttgct tctgacaatt
      181 gctttgcgcg gcgtgtccac cggcgaaact cgtcaacctg gcgccgatcg acttttggct
      241 tttcgttttt gtaagccttg tcgctgtaga gcgtcgactt cagttcccac gattcgttta
      301 gctgctgaat ccacaggctg gctgcgaaag cttgcgggcc ggcgggatga tccggcgcga
      361 ggtcgatcat cttttgaaac cggcgccgtg ctccttcgta gtcgagattg tacagcgctt
      421 cgtaaccctc ggccctcagt gtgtcgaggt cctgtgttgg ttcagcggcg ggttgagcga
      481 ccactgtggt gaaagaaaca aggagaatga gtatgcacgt ccagaacatt ggtggtgtac
      541 ttcttaaccg gtaaatcatg gtgctaccgg cagtgcggtg acacttgaat aactgccaca
      601 gatcatatca tccgtccaat ccgtggttgc taacctcccg atatgatcgc gcagcgacga
      661 caaattttcc acctcagccg agctcttatc tgcgtcatct gcgcgttatc gattggctct
      721 ttactggttc ccatagccaa caaatttcac ctggcgctcg atatcctgct tcgcacgtat
      781 cttatttttc tcggcaccgt gatggcccac gaaagtgttc acggccacct ggggagcacc
      841 cgacgcgcaa acttctggtg gggccgcatc gctttactgc ccagcatggt tcccttcacc
      901 aactttcgcc gcactcacca actacatcac gcctacacaa acatccccga caaggacccg
      961 gaccacttta tgaagtcgcg aaatcttctc gaactttcgt tgcgggccat cgcgatgccg
     1021 catcactggt tcttctggct gcgcaaacgc ggacgattga aacgggccga tttgtgggaa
     1081 ctgtttctga attatttggc gattgtggcg ctgtatgcaa ccgttctcag tttcgttgga
     1141 ccaacacggc tgctctgggg catggcgccg ccgctcattc tcgtttcgct gttgctctgg
     1201 tatccattcg cggtgcaaac gcatgaaggc ttctcgaccg gttccgcaga agcacgctcg
     1261 catgattact acgggaactt catgtactgg ttctcgcttg gcctatcgat gcatcgccag
     1321 catcacctgc aaccgaatct ctcatggatc gagttgcggc gatacgtgca acctgcgccg
     1381 cgacgtagat ggtggtggcc gcagcgcgac atcagaatcg atgtgcagca gagtagtgat
     1441 gcagtttgag actacggacc gtttacccgg aaccgcaggc gtctcgcctg catctcacgg
     1501 cggcgctcaa ctcgtcgcca tgcaggcgag acacctgcgg tcccagcaga acggcgcctc
     1561 agttttcaaa ctgccgctgt cggtgctgga ctacgcgttc agcctgctcg actccgccga
     1621 ctcgccgcag gatttcacca tcatccttca tttaggcaat ccgccgtcac ttgaaaacct
     1681 acgcgccggc tcgaggagtg cgacaaatcg gtatccgatc agtggctgca cgattaaaaa
     1741 cagaagttgg gtcttcggcg gtaacgaacc tgttcaactt ccctgcgacg gaaacggcaa
     1801 ccgttcactc gaatcgtttg tcaatgaacg tttcgacgtg cgcgaggaag ttccagtcag
     1861 gcaagtgctg tttgccaaca atgaagctga gcgctgtctc gtcacgcgat ttcatcacgc
     1921 ggccgcggat ggtttgagtg ccgcactgtg gttgggccat cagctcagtg tggcttatga
     1981 acttatcgca ccggagacgg catgcgcacg acccgttgaa ccaccactcc gcgaatccga
     2041 cagttcagtt cgccgaagtc tcttcgctta cagcggcgca tcagatccac tgtggaccac
     2101 aaactatatt ccttcaggcg cacggcgctg gctaaccatc aactttcccg cgaacgatct
     2161 ccggaaaggc tgtcgccgcg cgcgcggttt tacgtacaac gatctgctcg ccacgtgtgt
     2221 gcttgaagtg ctttccaact ggaatcgaaa aaacgatcct aaggcaacgc agaagatcgg
     2281 actatggtat ccgctaaaca tacgcgccaa ttccaatccg aattttggta acgccaccag
     2341 ccgcattcga ctctacgcga agttcccagc gggtgcgtcg ctcatcgata aggcgcggga
     2401 ggttcggaaa caggtttcat ggtcaacgtc gaatggcgaa tgggtccttc cgcagttg
//
LOCUS       DASTEY010000406         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_58331, whole genome shotgun sequence.
ACCESSION   DASTEY010000406 DASTEY010000000
VERSION     DASTEY010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58331"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /gene="sufD"
                     /locus_tag="VFO72_07205"
     CDS             complement(<1..1275)
                     /gene="sufD"
                     /locus_tag="VFO72_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET9479111.1"
                     /translation="MVTEVAKIENNYVSAFCAVRESSPTVPWLELVRRSAMDRFEQLG
                     FPSVHDEEWKYTNLAALAKQTFVPASRTENVSLDTTRFVYPETTTAHLVVVNGFYSEK
                     LSSTTGLENVVAIDLLTAVSDARYNKITRAYLARNAGYHNNGLAALNTAFLQSGLFLW
                     IPKNVNVETPIQITFVTDAEQADGASFPRLLIVAEENSSATVIESIVSTGEQQYFTDA
                     VAEIVVKEGARLEHYRMQRESKEAFHTSITSAELGRSSSYNTTSINLGGRLARHDISV
                     VMDNEGAECWVDGLYLIGSDQHTDTHSVIDHQQPNCTSHQLYKGILDGNARAVFNGKI
                     FVREGAQKTDAIQTNKNLLLSPQARVDTKPQLEIYADDVKCAHGAAVGQLDEEELFYL
                     QTRGINPELGRSLLTYGFAEEVIEKIKLVSIRS"
     gene            complement(1279..2064)
                     /gene="sufC"
                     /locus_tag="VFO72_07210"
     CDS             complement(1279..2064)
                     /gene="sufC"
                     /locus_tag="VFO72_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET9479112.1"
                     /translation="MLEIRNLHAAIDGNEILKGINLTVKKGEIHAIMGPNGSGKSTLA
                     KVLAGHPQYEVTKGEVIYEGRNLLEMSPDERARAGVFMAFQYPVEVPGVSNAQFLRLA
                     YNEKRKHLGEEELDPLEFKDLLKERAKVVEMDASFMTRSVNEGFSGGEKKRNEILQMA
                     VLEPKLAILDETDSGLDIDALRIVAGGVNQLLTPDNAIIVVTHYQRLLNYIVPDYVHV
                     LAGGRIAREGGKELALELESKGYDWITSATQDAASPAKLNAAN"
     gene            complement(2065..>3280)
                     /gene="sufB"
                     /locus_tag="VFO72_07215"
     CDS             complement(2065..>3280)
                     /gene="sufB"
                     /locus_tag="VFO72_07215"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET9479113.1"
                     /translation="IDYQNIIYYSAPKQKATKASLDEVDPELLRTFEKLGIPLQEQKQ
                     LAGVAVDAVFDSVSVATTYKEKLKKHGVIFCSFTEAIRDYPELVKKYLGSVVPANDNF
                     FAALNSAVFSDGSFCFIPKGVRCPMELSTYFRINNAESGQFERTLIVAEEGSYVSYLE
                     GCTAPKFDRNQLHAAVVELVALDDAQIKYSTVQNWYAGDEEGRGGIYNFVTKRGKCAG
                     KNSKISWTQVETGSAITWKYPSCILVGDNSIGEFYSVALTNHAQQADTGTKMIHLGKN
                     TRSTIISKGIAAGKSNNSYRGLVKVMPKAEGARNYTQCDSMLIGSSCGANTFPYIEVM
                     NNSSSVEHEASTSKISEEQLFYLQQRGISQEDAVSLIINGFCKDVFLQLPMEFAVEAT
                     RLLGLKLEGSVG"
BASE COUNT          686 a          927 c          846 g          821 t
ORIGIN      
        1 agacctgatc gagacaagct ttatcttctc aatcacttct tcagcaaagc cataggtcaa
       61 cagactgcgg cccagttccg gattgatgcc gcgcgtctgg aggtaaaaca actcttcctc
      121 atcgagctgg ccgacagcag cgccgtgcgc gcatttcacg tcgtcggcat aaatctccaa
      181 ctgtggtttc gtatcgacgc gagcctgcgg tgacagcaat aggttcttgt tggtctgtat
      241 cgcatcagtc ttttgcgcgc cttcgcgaac gaatattttg ccgttgaaga ccgcgcgcgc
      301 gttgccgtcg agaattcctt tgtacaactg gtggctggtg caattcggtt gctggtgatc
      361 gattaccgaa tgcgtgtcgg tgtgctgatc ggagccgatg agatagagac catcgaccca
      421 acactctgcg ccttcgttat ccatcaccac agaaatgtcg tggcgcgcca agcggccgcc
      481 gagattgatc gaagtggtgt tgtagctgct gctgcgtccg agttcggctg aagttatcga
      541 cgtgtgaaaa gcttctttac tttcgcgctg catgcggtag tgttcgagcc gcgcgccctc
      601 cttgacgacg atctccgcaa ctgcatcggt gaaatactgc tgctcgccgg tcgaaacaat
      661 gctctcgatt actgtggccg aactattctc ctcggctacg atcagcagcc gcggaaaact
      721 cgcaccatcc gcctgctccg catccgtcac aaacgtgatc tgaatcggcg tttcgacgtt
      781 tacgtttttc ggaatccaaa gaaacaagcc tgactggaga aacgcagtat tcagcgcagc
      841 caaaccgttg ttgtgataac cggcgtttcg cgcgagatat gcacgcgtga ttttgttgta
      901 tcgcgcatca gacactgccg tgagcagatc gatggcgacg acgttctcaa gcccggttgt
      961 gctggagagt ttctcggagt agaagccatt aacgacgacg agatgcgcgg ttgtcgtttc
     1021 agggtagacg aaacgcgtag tgtcaagcga aacgttttcc gtacgactcg ctggaacgaa
     1081 agtttgcttc gcgagcgcag ccaggttggt gtatttccac tcttcgtcgt gaacggaagg
     1141 aaagccgagt tgctcaaaac gatccatcgc actgcgacgc accagttcca gccacggcac
     1201 cgtcggactc gactcgcgca cggcgcaaaa cgcactaacg taattgttct ctatttttgc
     1261 gacttcagtg accattgctt aattcgccgc gttcaacttt gcggggctgg ctgcatcttg
     1321 cgttgccgac gtaatccagt cgtagccttt cgactcgagt tcaagcgcca actccttccc
     1381 accctcgcgc gcgatccggc caccggcgag cacgtgcacg taatccggca cgatgtagtt
     1441 caacagtcgc tgatagtgag taacgacaat gatagcgttg tcgggcgtca atagttggtt
     1501 cacgccgcct gcgacgatgc gcagcgcgtc gatgtcgagg cccgagtctg tttcgtcgag
     1561 gatggcaagc tttggctcga gcacggccat ttgcagtatc tcgttgcgct tcttctcacc
     1621 gccggagaaa ccttcgttga ccgaacgcgt catgaaactc gcgtccatct caacgacttt
     1681 cgcccgctcc ttcagcagat ctttgaactc cagcggatcg agctcttctt cgccgagatg
     1741 cttgcgtttc tcgttgtagg ccaggcgcag gaactgagcg ttcgagacac ccggcacttc
     1801 gaccggatac tgaaacgcca tgaacacgcc tgcgcgagcg cgttcatcag gcgacatctc
     1861 caaaaggttt cgcccttcgt agatgacttc gccttttgtg acttcgtatt gcggatggcc
     1921 cgcaagcact ttcgccagcg ttgatttgcc cgaaccattc gggcccatga tggcgtgaat
     1981 ctcgcctttt ttgactgtta aattgatgcc tttgagaatt tcattaccgt cgatcgccgc
     2041 atgtaagttt ctgatctcta acatttatcc cacactacct tccaacttca gacccaacag
     2101 ccgcgtcgct tcgacggcaa actccattgg caactgtaga aagacatctt tgcagaaccc
     2161 gttgatgatc aacgacaccg catcttcctg cgagatccca cgctgctgca gataaaaaag
     2221 ttgttcctcg ctgatctttg aagtcgatgc ttcgtgttca acgctggagc tgttgttcat
     2281 cacttcaata tacggaaacg tgtttgcgcc gcagctcgat ccgatcagca tcgagtcaca
     2341 ctgcgtgtag ttgcgcgcgc cttcggcttt cggcatcact ttcacaagtc cgcgataact
     2401 gttgttcgat ttccccgccg ctattccttt ggagatgatg gtcgagcgag tgttcttgcc
     2461 cagatggatc atcttcgtgc cggtgtcggc ctgttgcgcg tgattggtca aggcaaccga
     2521 gtagaactca ccgattgaat tgtcaccgac gagaatgcac gacgggtact tccaggtgat
     2581 tgccgatccg gtttcaactt gcgtccagga gatctttgag ttcttacccg cgcacttacc
     2641 acgcttcgtg acgaagttgt aaatgccgcc gcgtccttct tcatcgccgg cataccagtt
     2701 ctgcactgtc gaatatttga tctgtgcgtc gtcaagcgcc accagttcaa ccacggccgc
     2761 gtgcagttgg ttgcgatcga acttcggcgc cgtacaaccc tcgagatacg aaacgtacga
     2821 accctcttcg gccacgatca gtgtgcgctc aaactggccc gactccgcgt tgttgatgcg
     2881 gaagtaggtc gagagttcca tcggacaacg cacacctttg ggaatgaagc agaagcttcc
     2941 atccgagaag accgcgctgt tcagcgccgc gaagaaatta tcgttcgccg gcacaacgga
     3001 accgagatac ttcttcacca gttccggata gtctctgatc gcttccgtga acgaacagaa
     3061 gatcacgcca tgctttttga gtttttcttt gtacgtcgtg gctactgaaa ccgaatcaaa
     3121 cacggcatca accgcaacgc cggccaactg tttctgttct tgtagtggaa tgccgagttt
     3181 ttcgaacgtg cggagcagct ccggatcgac ttcgtcgaga ctggccttcg tcgccttctg
     3241 tttcggcgcg ctgtagtaga tgatgttttg gtaatcgatc
//
LOCUS       DASTEY010000407         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_107637, whole genome shotgun sequence.
ACCESSION   DASTEY010000407 DASTEY010000000
VERSION     DASTEY010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107637"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..2521)
                     /locus_tag="VFO72_07220"
     CDS             complement(119..2521)
                     /locus_tag="VFO72_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479114.1"
                     /translation="MIKDIRYALRTLLKRPGFLLVALSTLALGIGATTAMFTVVNSVL
                     LRPLEFPEPERIVLMEGVNPRMGITRSNMSVPDVVDWQQQAQVFEQIAGFITWGAFLA
                     TGDETERVRAAGVSPEFFPLFRTPPLSGRTIQSDDFKPESGPVTVISYALWQRRFGGA
                     ADVINRKVTLSGTTNTIVGIMPAGFNYPADTELWTAFPLDASEEVRDNRFLNVLSRLK
                     PGVSIAQAQTEMDTINQRLAQNYAETNTGWSVRLTELRERMVGELRTSLLMLFGAVAF
                     VLLIACANVANLLLARAAYRQKEIAVRTALGASRLRIVRQLLTESVLLSVVSGAIGLG
                     LSVWLIKLLVAISPPDTPRFEEISINWQVFVFAFGVTILAGLLFGLVPALQTSRTNLN
                     ETLKESGRTGAHSGTINRVGSFLIVSEVALSFVLLAGAGLLIKSFMHLRKIDPGFNSE
                     NVLAVRLTLPPGKYKQGEPRAQIYKQLVDQVKTVPGVQSAGVVLSLPLGGDTFNVGRG
                     VLLEGRPAKAEEGSNAAYLVATPDYFNTLQIPLKQGRAFTAHDDAQSAKVVIVNETMA
                     RQLWPGQSPVGRRFSIWRDEKFSREVIGVVGDTKSVLDREARPQMYVPYAQDANWGSL
                     SLVARTSGEPSAMAASVREAIRSVDKGVATYNLKTMNDVVSTAAAPRRVPMLLLSSFS
                     GVAMLLAMIGIYGITSYYVTQRTHEIGVRMALGAQIADVLKLVLTRAMLLAVIGVGVG
                     LAGAMAVTRYLSTLLFGVQPIDVITFAAVALGLVLVALVASLIPAKRATKVDPLTALR
                     YE"
     gene            complement(2677..>4076)
                     /locus_tag="VFO72_07225"
     CDS             complement(2677..>4076)
                     /locus_tag="VFO72_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9479115.1"
                     /translation="APWLVTLIVKSQSRLDIAGTLLGQSVDTRVLAFTALTTVVAGLA
                     FGFLPAWQSSRAQLAPSLKDEGGLSNQRDRRFNLRSLMVVGQLAVAVVVLIAAGLSIR
                     SLRNLLAVDPGYESENLLIIPLELDDKKYDEARGAVFQRQVFERLAKLPGVEALSYGL
                     VMPFSGSKYMASIFVEGGQPLPDQQMAFDASVVGPGYHETMGIKFVAGRGFTDQDTGG
                     APKVIVINESLARLLFPGEAALGKRLMLRTDGPPLEVIGITKGIKHHELTEAPLPHFD
                     LPALQRGYDGYTNIVLRTRIDPRELTRAVRAELLGMDPSLPVSDITPMSIQIRNALAG
                     TSLASSLVGLFGFLGLLLASIGLYGVMAWMVSRRTREVGIRIALGARPRDVLRLVIGQ
                     GLILTMSGLLIGLVAAFIATRLIDTQQLYGISPTDAFTFAGGAIVLTVVSLLACYIPA
                     RRATKVDPLVALRYE"
BASE COUNT          929 a         1258 c         1075 g          814 t
ORIGIN      
        1 ccggacgttt ccacaatccg cgcacaccat accgaagatc ttttataaag ttgtccatta
       61 accttgtaac accgcaccac tgtgaaatgt gaccacagat ctgccctcat ccgtggcctt
      121 attcatatct gagcgctgtt aacggatcta cttttgttgc ccgttttgcg ggtatcaaag
      181 acgccaccaa cgccaccagc accaatccca gcgccaccgc cgcaaatgtg atcacgtcga
      241 tcggttgaac gccgaacaac agcgtcgaca ggtaacgagt caccgccatc gcaccagcga
      301 gaccgacacc aacgccaatc actgcgagca acatcgcgcg cgtcaacacg agcttgagca
      361 cgtcggcgat ctgcgcgccg agcgccatgc gcacaccgat ctcatgagtt cgttgagtga
      421 cgtagtagga agtgatgccg tagatgccga tcatcgcgag caacatcgcg acgcccgaaa
      481 acgagctcaa cagcaacatc ggcacacgtc gcggcgcggc cgccgtggag accacgtcgt
      541 tcattgtctt caggttgtaa gtcgcaactc ctttatcgac cgaacggatc gcctcgcgca
      601 ccgatgcagc cattgcggac ggctcaccgc tcgtgcgagc taccagcgag aggctgcccc
      661 agttcgcatc ctgcgcgtaa ggcacgtaca tctgtggtct agcttcgcgg tcgagcactg
      721 acttggtgtc gcccacgaca ccgatcactt cgcgcgaaaa cttctcgtcg cgccaaatac
      781 tgaagcggcg gcccacaggg ctttggccag gccacagttg ccgcgccatg gtctcattga
      841 caatcacgac tttcgctgac tgcgcgtcgt cgtgggccgt aaatgcgcgt ccctgtttta
      901 acgggatctg aagcgtgttg aagtagtcag gggtggcgac caggtaggct gcattcgatc
      961 cttcctcagc cttcgcaggg cgcccctcga gcaggactcc gcggccgacg ttgaacgtgt
     1021 caccaccaag tggcagactc aataccacgc ccgcgctttg cacaccgggc actgtcttca
     1081 cctggtcgac gagttgtttg tagatctgcg ctcgcggttc gccttgcttg tacttgccgg
     1141 gtgggagagt caagcgcacc gcgagcacgt tctccgaatt gaatcccgga tcgatcttgc
     1201 gcaggtgcat gaagctcttg atcagcagcc ccgcaccggc caacagcacg aacgagagcg
     1261 caacctccga cacaatgaga aaactgccga cgcgatttat tgttccgcta tgcgcgccgg
     1321 ttcgtccgct ctctttcaga gtctcgttga gattggtgcg cgaagtttgc agcgccggca
     1381 cgagaccaaa tagcaggccc gcgagaatcg tgacgccaaa cgcaaaaaca aagacctgcc
     1441 agttgatcga gatctcttcg aaacgcggag tgtccggcgg gctgatcgcg acgagcaact
     1501 tgatcaacca aacactcaac ccgagaccaa tcgcgccact cacgactgaa agcagaacgc
     1561 tctcagttaa gagttgccgc acgattcgca gacgactcgc gcccagagcc gttcgcactg
     1621 cgatctcttt ttgccggtat gccgcgcgcg cgagcaacag gttggccacg ttggcgcacg
     1681 caatcaacag cacaaatgcc acggcgccaa ataacatcag cagcgacgtg cgcagttctc
     1741 caaccatccg ctcccgcaat tctgtaagac gaacgctcca acccgtattc gtctcggcat
     1801 aattctgcgc gagccgctgg ttgatcgtat ccatctctgt ctgcgcctgc gcgatcgaca
     1861 cgccgggttt cagccggctg agaacgttca gaaaacggtt gtcacgcacc tcctcactgg
     1921 catccagcgg gaatgcggtc cacagctctg tatcggccgg atagttgaaa ccagcgggca
     1981 tgatgccgac gatcgtgttg gtcgtgccgc tcaacgtgac tttacgattt atgacgtcag
     2041 ccgctccgcc gaaacgtcgt tgccacagcg cataactaat tactgtcaca ggaccacttt
     2101 cgggtttaaa gtcgtcggac tggatggtgc ggccgctgag cggcggggtc cgaaagagcg
     2161 gaaagaattc aggcgagacg cccgcagcgc gcacgcgctc agtctcatcg ccggtcgcca
     2221 gaaacgcgcc ccacgtaata aagccggcga tctgttcgaa gacctgggcc tgctgttgcc
     2281 agtcgacgac atctggcact gacatgttgc tccgcgtgat ccccattcgc ggattaacac
     2341 cctccatgag cacgatgcgt tccggttcgg gaaactcgag cggcctcagt aagacggaat
     2401 tcacgaccgt aaacattgcg gtcgtcgcgc cgatgcccaa tgcgagggtt gacagtgcga
     2461 cgagcagaaa gcccggccgc ttcagcagcg tccttaacgc ataacgaata tctttgatca
     2521 tctgcgtaac tccgtggttc tactcgtttc tcggcgctac ccgcggatcg acttttgtgg
     2581 cccgccgtga aggcacgcaa gcagcaacca gcgcgacggc aacgagcagg accgacatga
     2641 atgaaaagtc gtcatcattt ctaatgttcc tattgcctac tcatatctaa gcgcgactaa
     2701 aggatcgacc ttcgtcgccc gtcgcgccgg tatgtaacag gccagcagtg aaactacggt
     2761 cagcacaatc gctccccctg cgaatgtgaa tgcatccgtc gggctgatgc cgtagagctg
     2821 ttgagtatcg atcaggcgcg tggcgatgaa cgcagcaacc aaaccgatta aaagccctga
     2881 cattgtgagg atcagcccct gtccaatgac tagccgcagt acgtcgcgcg gtcgtgctcc
     2941 cagcgcgatg cgaataccca cttcgcgcgt gcgacgactc accatccaag ccatgacgcc
     3001 gtataaaccg atactcgcca gcaacaatcc gagaaaacca aaaaggccga cgagagatga
     3061 cgccaggctt gttccggcaa gtgcgttccg gatctgaatc gacattgggg tgatatcact
     3121 aaccggaagt gatggatcca ttccgagcag ttcagcccga acagctctcg ttagctcccg
     3181 tggatcaatg cgggttcgca acacgatgtt tgtataaccg tcgtaaccgc gctgcagggc
     3241 gggaagatca aaatggggca acggagcctc ggtaagctcg tgatgcttga tgcctttcgt
     3301 gataccgatt acctcgagtg gtggtccatc cgttctgagc attagccttt tgcccagcgc
     3361 ggcttcacct ggaaacagca ggcgtgcgag tgattcattg atgacgatca ccttaggcgc
     3421 tccgccggtg tcctgatccg tgaacccgcg tcccgccaca aatttgatgc ccatagtctc
     3481 gtggtaacca ggaccaacaa cactcgcgtc aaacgccatc tgttgatccg gtaacggctg
     3541 gccgccttcg acaaagatgc tggccatgta tttgctacca ctgaacggca ttaccaggcc
     3601 gtaactcaac gcctccactc ccggcagttt tgcaagacgt tcaaacacct gtcgttggaa
     3661 aactgctccg cgagcctcgt catacttttt atcgtccaac tcgagtggaa tgatgagaag
     3721 attttcagat tcgtatccag gatcaaccgc cagcaggttg cgcagactgc gaatcgatag
     3781 accggcagca atcaggacca ccaccgcaac cgccagttgg cccaccacca tgagactgcg
     3841 caggttgaag cggcggtcac gttgattcga caacccgccc tcgtctttca gcgacggcgc
     3901 caattgagct cttgaactct gccacgcggg aagaaatcca aacgctaacc ctgcgacaac
     3961 tgtcgtcaac gccgtgaatg ccagtacgcg agtatcgacg ctctgtccca gcagtgtccc
     4021 agcaatgtct aatcgcgatt ggctcttaac gatcagcgtc actaaccagg gagcaa
//
LOCUS       DASTEY010000408         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_899083, whole genome shotgun sequence.
ACCESSION   DASTEY010000408 DASTEY010000000
VERSION     DASTEY010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899083"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2618)
                     /locus_tag="VFO72_07230"
     CDS             complement(<1..2618)
                     /locus_tag="VFO72_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479116.1"
                     /translation="MIGQTVSHYRILEKLGEGGMGVVYLAEDQHLARRVAIKFLTSTD
                     HHYRARFIREARAVSALTHPNIAIVHDYGETSTGQPFLVMEFVKGKSLSELLEEGLTL
                     RRSVEIVSSIAEALAEAHHHGIVHRDIKPSNILVNERGVVKVLDFGLVKHLFEPPSSE
                     VDLDAQTIFSTQTRSDVIVGTPLYLSPEQATGRAIDGRSDIFALGALLYECLTGRSAF
                     AGGSVLEIGAQIIHVTPPIPSQVNAQVTPALDRVTMKALQKKVEDRYQSAGEMLKDLQ
                     AAIVGLGGNGVPVSSKSVRPTSGEMHRATSALATLTMQLRRQRFSLVSFIATILLSGL
                     VIWAIVHFWPRTYYQPSAAALKWYEIGAEGVRNGAYHQASKAFQQAIGIDSNYVLARA
                     RLAQAWTELDYMENAKDELLAITSLTRNGAAISPKDALYLDAITAMTRRDFREAVKAY
                     SAIAEMSPEESQVYVDLGYAYENDGNPEKALENYLKAIPLNNGQYATAYLRAGIVYNR
                     KQDRSKAEEMFDKAEELYRAASNNEGVNEVLRQRGTLYRETGRYNEARAQFQQGLDAA
                     RATGLEAQQINALIDLSYLASNIGEPEAESYAQQAVTIAQQKQLDNLAAAGLNELGIS
                     YHFKQDYAKAEHYFSEAIQLARANKGRVAEARGMSNLAGVYIATLRVDQGLELARQAL
                     DFFKEKNYPQRASFCLTHMVRGNRRKGDYATALKWANEKLEIAKQGSAQPTIASSYVE
                     IGAVLMEREDLPAALKQYDDALNIYKDLNNKLQISFNNANRGNILWHLGHYDQAESIF
                     EEVSKTMAESKNTYKQLFPSVLLYRAQLRLSQRNLRDAIALGNEAITAAGEQIPDVAI
                     EGASVLGLAQALSGNA"
     gene            complement(2679..3170)
                     /gene="purE"
                     /locus_tag="VFO72_07235"
     CDS             complement(2679..3170)
                     /gene="purE"
                     /locus_tag="VFO72_07235"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9479117.1"
                     /translation="MSTHKSLVAVIMGSQSDWETMRHADETLSQFGVAHECRVISAHR
                     SPSLAAEFGSTAEERGLEVIIAAAGGAAHLAGVLAAHTTLPVLGVPMKSDALNGLDSL
                     LATVQMPAGIPVGTLAIGKPGAINAALFAVAILANSRPELRQKLHEFRESQEAKLRAT
                     KLT"
     gene            complement(3172..>3576)
                     /locus_tag="VFO72_07240"
     CDS             complement(3172..>3576)
                     /locus_tag="VFO72_07240"
                     /inference="COORDINATES: protein motif:HMM:NF037786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479118.1"
                     /translation="RKYKKAEAEAASTTPAAQKPAPQKELVGVDVFLDWTKGSANDLG
                     DGLSRLNGEGLKLTMISNRGVKVWPGGHTETFCSDHWRCRYLSESDGGKVSHAQIISL
                     LGRVAGEGYDFVKTEGLYTFDGERGFSLDQGE"
BASE COUNT          733 a         1040 c          973 g          830 t
ORIGIN      
        1 tttgcatttc cagacagcgc ctgcgccaaa cccaaaacgg acgctccttc gatcgccaca
       61 tctggaattt gctcgccagc ggctgtaatt gcctcgttgc cgagcgcgat cgcgtcgcga
      121 aggtttcgct gactcaaacg cagttgcgcc cggtacagca gcactgacgg aaaaagctgc
      181 ttgtaggtgt tcttcgactc agccattgtc tttgacactt cctcgaaaat cgactctgcc
      241 tgatcataat gacctaaatg ccataatata tttccgcgat ttgcgttgtt gaacgatatt
      301 tgcagtttgt tgttcaggtc tttatagatg tttagtgcgt cgtcgtactg tttcaaggcg
      361 gcgggaaggt cctcgcgttc catcagaacc gcaccgattt caacgtagga gcttgcgatc
      421 gtcggctgag cactgccttg tttcgcaatc tcgagttttt cgttggccca tttcagcgca
      481 gtagcataat cacctttccg gcgattgccg cggaccatat gggtcaaaca aaacgaagca
      541 cgttgcggat agtttttctc cttgaaaaag tccaacgcct gccgcgcgag ttccaggcct
      601 tgatcaacgc gtagcgtcgc aatgtagact ccggcgagat tgctcatacc acgagcttca
      661 gcgacgcggc ctttattcgc acgagccaat tggatcgcct cgctaaaata gtgttcagcc
      721 ttagcgtagt cctgtttgaa gtgataagag atgccgagct cgttcagtcc ggccgcagcg
      781 agattatcga gttgtttctg ctgggcaatc gtgaccgcct gttgcgcata tgattcggct
      841 tcaggctccc ctatatttga cgcgaggtaa ctcaaatcga tgagcgcatt gatctgttgt
      901 gcttcgagac ccgtcgctct ggccgcgtcg agcccctgct gaaattgagc gcgcgcttcg
      961 ttgtaacggc cggtttcgcg atacagcgtg ccacgctgtc gcagtacttc gttcactcct
     1021 tcattgttac tcgcggcgcg gtacaactct tccgccttat cgaacatttc ctccgctttg
     1081 gaccgatcct gtttgcggtt gtagacaatt cccgctctca ggtatgcagt cgcatactgg
     1141 ccgttattga ggggaatcgc tttcaaataa ttctcaagcg ctttttcagg gttgccgtcg
     1201 ttctcatagg cataacccag gtcgacatag acctgactct cttcgggcga catttcagca
     1261 attgccgagt aagccttaac cgcttcacga aaatcgcggc gcgtcatggc ggtgattgcg
     1321 tcgaggtaaa gcgcatcttt cggtgagatc gcggcgccgt tacgtgtcag gctcgtgatt
     1381 gccagtagtt catctttcgc gttttccatg taatcaagct ccgtccatgc ttgcgctaga
     1441 cgcgcacgcg ccaaaacata attgctatca atgccgattg cctgctggaa ggctttgcta
     1501 gcctgatggt aagcaccgtt tcgcacaccc tcggcgccga tttcatacca cttgagggcg
     1561 gccgcggagg gctggtaata agtgcggggc cagaagtgaa cgatggccca gatcaccaaa
     1621 ccactcaaca gaatggttgc aataaacgag acgagcgaaa aacgctgccg ccgaagttgc
     1681 atcgtgagtg tcgccaaggc gctcgtggcg cggtgcatct cacccgacgt cggtcgaact
     1741 gatttgctcg acacgggaac gccattgccg cccaggccga caatcgccgc ctgcaagtct
     1801 ttcaacatct cgcccgctga ttgataacgg tcctcgacct tcttttgcag cgctttcatc
     1861 gttacccgat cgagcgcggg cgtcacctgc gcgttgactt gggacggtat cggaggcgtt
     1921 acgtgaatga tctgtgcgcc gatctccagc acgctgccac cggcaaaagc tgaacgtccg
     1981 gtcaggcact cgtacaacag cgcgccgagc gcgaagatgt cactgcggcc gtcgattgct
     2041 ctgccggtag cttgttcggg cgagagatat agcggcgtac cgacgatgac gtcgctgcgg
     2101 gtctgcgttg aaaaaattgt ttgcgcgtcg aggtcgactt cactggacgg cggctcaaac
     2161 aggtgtttca ccaggccgaa gtcgagcact ttcaccacgc cacgttcgtt cacgagaatg
     2221 ttcgagggct tgatgtcgcg gtggacgatg ccatgatggt gcgcttcggc gagggcctcg
     2281 gcgatcgaag agacgatttc gaccgaacgt cgcaaggtca gcccttcttc cagcagctcg
     2341 ctaagcgact tgcctttaac aaactccatc acgaggaacg gctgacccgt cgaggtttcg
     2401 ccgtagtcgt gcacgatcgc gatgtttggg tgcgtgagcg cggaaacagc gcgagcttca
     2461 cgaatgaaac gagcgcggta gtgatggtct gtggaagtga gaaacttgat cgcgacgcgg
     2521 cgcgcgaggt gttggtcttc cgccaggtag acgacgccca tgccgccttc gcccagtttt
     2581 tcgaggattc gataatggga aaccgtttgg ccgatcatag tcaaccgcgg aggagcaagg
     2641 attagacgga catagagtaa accaggttac tctggaattc acgttagttt ggtggcgcgg
     2701 agctttgctt cctgcgactc gcgaaactca tgaagcttct gtcgcaactc cgggcggctg
     2761 tttgcaagga tggccacagc aaaaagcgcc gcgttgatgg cgccgggctt gccgattgcc
     2821 agcgtaccga cgggtattcc agcgggcatt tggacggtcg ccagcaatga gtccaggcca
     2881 ttcagagcgt cgcttttcat cggaacgccc agcaccggca gcgtcgtgtg tgcagcaagg
     2941 acgccggcga ggtgcgccgc tcctccagcc gccgcaatga tcacttcaag tccgcgctct
     3001 tctgccgtcg aaccaaactc cgccgccagc gaaggcgacc ggtgcgccga gataacgcgg
     3061 cactcatgcg ccacgccaaa ttgagacagc gtctcatccg catgccgcat tgtttcccaa
     3121 tcagactgag aacccatgat cacagctacc aacgacttgt gtgtgctcat cttattcgcc
     3181 ctggtctagt gaaaacccgc gctcgccatc gaacgtgtac aaaccttctg tcttcacgaa
     3241 atcatagccc tcgccggcga cgcgccccag caacgaaatg atctgcgcgt gcgatacctt
     3301 gccgccgtct gattccgaaa gataccggca acgccagtga tcggaacaga acgtctccgt
     3361 gtgaccgccg ggccagacct taacgccgcg attgctgatc atcgtcaact tcaacccctc
     3421 gccattcaac cggctcaatc catcgcccag gtcgttcgcg gagcctttgg tccaatcgag
     3481 aaacacatcg acgcccacca gctctttttg cggtgccggt ttctgcgcag ccggagtcgt
     3541 gctcgcagcc tccgcctcgg ccttcttgta ctttct
//
LOCUS       DASTEY010000409         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_338452, whole genome shotgun sequence.
ACCESSION   DASTEY010000409 DASTEY010000000
VERSION     DASTEY010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338452"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..954
                     /locus_tag="VFO72_07245"
     CDS             187..954
                     /locus_tag="VFO72_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479119.1"
                     /translation="MNAAQFNYYTERLRISLESRDEVVGLVVLGSTADPTLRDEWSDH
                     DFWVITKPGAQDALVEDLSWLPEYQDIAISITHRPHGRTILLRNRHKVEFAVFNVDEM
                     RSGKVERYRVLTDRDQIAELIKAVHEESLKQARTGSETLQNLCLLLWSACERHYRGEL
                     LSARQYLDAFAVNQLSSLLSDSEPGDARKDALDPRRRLEMRSPALAAELLTTLNKPVP
                     AAALSLLQLVERELKPRAPTLPWDKVDMVRGWIAERT"
     gene            983..1258
                     /locus_tag="VFO72_07250"
     CDS             983..1258
                     /locus_tag="VFO72_07250"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET9479120.1"
                     /translation="MIIAGNNSGFELVFKEGMVVSRSVGLLVVIGGVLVILVGLLIYS
                     GALNWFGRLPGDIRYESGSVRVYIPIVSMLLISVVLTLIFNLVRRLF"
     gene            complement(1270..>2447)
                     /locus_tag="VFO72_07255"
     CDS             complement(1270..>2447)
                     /locus_tag="VFO72_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9479121.1"
                     /translation="STINGPLGIGFQPFGGTTRSDFDARIHTLNARVDVQAGSANYIT
                     AGYEFERENFKNPSFQVNPADNSDVDVTQRSHALFVQDQLRLFDDRLQISGAFRAQFF
                     RLERPVFTPAASAPYTGFIFQSPPAAYTGDGSIAYTFRSSGTKLRAHAGNGYRAPSLF
                     ERFGSFFDSFFGYSVLGDPRLEPERSIGFDVGIDQAFHENRVRVSATYFYTQLRKVII
                     FDFSGLIDFTTDPFGRFGGYLNANGGIARGAEVELRATPTRTLNLTAAYTFIKARQRT
                     PQVPGTLRSLVVPDHQFSLVATQRFGRRLLVNFDFSASSNYLGAVFDTVTSISRGYRF
                     KGLARADIGASYSLPLGREKRQLRFFGYVDNLFDREYFESGFRTPGRTGRAGASFSF"
BASE COUNT          562 a          635 c          704 g          546 t
ORIGIN      
        1 tctttccttc attgcggtga cggcaatgag ttttccgggg tttgttgtta acttacacag
       61 gccgcaaggc tccggtagaa gcgtgagtca aggtgcgagg taagtcgcgg tgcaaattta
      121 gcgcaagaag atcgttagaa caagttttta gcgcccgccg tctgggttga gaaagtaggt
      181 gttccaatga atgccgctca gtttaattat tacacagaac gactgcgaat ttcgctcgag
      241 tcgcgggacg aagttgtggg tcttgtggtg ttgggttcga ctgccgaccc gacgcttcgg
      301 gacgagtggt ccgatcacga cttttgggtg atcacgaaac cgggcgcgca agacgcgttg
      361 gtcgaagatc tatcgtggct accggagtat caagatatcg cgatctcgat tactcaccgt
      421 ccacacggca ggaccatact gcttcgcaac cggcacaaag tcgaatttgc ggtattcaat
      481 gttgatgaaa tgcgctccgg taaagttgaa cgttaccgag tgcttactga tcgcgatcag
      541 atcgctgagt tgattaaggc ggttcacgag gaatcgctaa agcaagcgcg aaccggatcg
      601 gaaactctgc agaatctctg cttgctgtta tggtctgctt gcgaacgcca ctatcgaggc
      661 gagcttttga gtgcccggca atatctcgat gcatttgcag tcaatcaatt gtcgagtttg
      721 ctctctgata gcgaacctgg cgacgcccgt aaggacgctc tcgatccgcg ccggcgtctc
      781 gaaatgagat cgccggccct agcggctgaa ctgctaacga cattgaacaa gccggtgcct
      841 gcagcagcgc tttcgttgct tcagcttgtc gaacgcgaac tcaaaccaag agccccgacg
      901 ctgccgtggg acaaagtaga catggtgcgc ggctggattg cagaacggac gtaaaccacc
      961 ttgatacaaa ctgtgtaagt aagtgattat tgcgggcaat aactcagggt ttgagctggt
     1021 tttcaaggag ggaatggtcg tgagtcgttc agtgggattg ctggtagtta tcggaggagt
     1081 tctcgtcatc ctcgtcggct tgttgatcta ctcaggcgca ctgaactggt tcggccgtct
     1141 gcccggagat atccgttacg agagcggcag cgtccgcgtc tacattccca tcgtctcgat
     1201 gttgctcatt tcagtcgtgt tgactctgat cttcaacctc gtgcgccggt tgttctaagc
     1261 aactctgaat caaaagctga aactcgcgcc ggctctgcct gtccgtccgg gggtgcgaaa
     1321 gccactctcg aagtactcgc ggtcgaagag attgtccacg taaccaaaaa aacgcagttg
     1381 ccgcttttca cgaccaagtg gcagtgagta actcgcgccg atgtcagccc tcgcgagacc
     1441 cttgaatcga tagccacggc tgatggacgt gaccgtatcg aagaccgccc cgaggtaatt
     1501 actcgaagca gagaaatcaa aattgacgag tagccgccgg ccaaaacgct gcgttgcaac
     1561 gagcgagaac tgatgatcgg gaaccaccag tgaacgaagc gtgcccggta cttgcggagt
     1621 gcgctgccgt gctttgatga acgtgtaagc cgcggtcaga ttcaacgttc tggtgggggt
     1681 cgcccttagc tcgacttccg cgccgcgcgc gataccccca ttggcattca gatatccgcc
     1741 aaagcgacca aacggatctg tggtgaagtc gatcagtccc gagaagtcga agatgatcac
     1801 tttccgcagt tgagtgtaga agtaggtcgc cgagactcgt actcggtttt cgtgaaacgc
     1861 ctgatcgatt cccacgtcga aaccgatgga acgttcgggc tccaaacgcg gatcacccag
     1921 aactgaataa ccaaagaagc tgtcaaagaa cgatccgaag cgttcgaaga gcgacggcgc
     1981 gcgatagccg ttgccggcgt gtgcgcgtag cttcgttccg gaagaacgaa acgtgtaagc
     2041 gatcgagccg tcgccggtgt aagccgcggg cggcgactga aatatgaagc cggtgtatgg
     2101 cgcggatgcg gcgggcgtga acaccggtcg ctctaatctg aagaactgcg cgcgaaatgc
     2161 gcccgagatc tgtaaacgat cgtcgaacag ccgcaactga tcctgcacaa acagcgcatg
     2221 gctcctctgc gtaacgtcga cgtcggagtt gtcagccgga ttgacctgaa acgaggggtt
     2281 cttaaaattc tcacgctcaa actcgtatcc agccgtgatg tagttggcgc ttcccgcttg
     2341 cacatcgaca cgcgcattca gcgtgtgaat tcgcgcgtcg aaatcggatc tcgtcgtacc
     2401 gccgaaaggc tggaatccta tgccgagtgg tccattaatg gtgctgc
//
LOCUS       DASTEY010000410         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1327159, whole genome shotgun sequence.
ACCESSION   DASTEY010000410 DASTEY010000000
VERSION     DASTEY010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327159"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /gene="atpD"
                     /locus_tag="VFO72_07260"
     CDS             complement(<1..1015)
                     /gene="atpD"
                     /locus_tag="VFO72_07260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET9479122.1"
                     /translation="MSTPDGKVTTDGQQVGKVVQIIGPVLDVEFQGGYLPPIYQALRV
                     VSEGFDVPTPIDVIAEVQQHLGEGRVRTVSMLPTDGMVRGMKAIDTGGPITMPVGEGT
                     LGRVLNVIGEPVDTLGPVKHEKRYPIHRHAPTFEEQSTELQMFETGIKVIDLIQPFLR
                     GGKIGLFGGAGVGKTVIVMELINNIAKARSGFSVFAGVGERTREGNDLLREMVESQVI
                     QFGDAFNKNFHDTGKFDLSKVDMNTIGQSKVALIYGQMTEPPGARLRVALSGLTVAEY
                     FRDQGMDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPNLATEMGEMQERITSTKT
                     GSVT"
     gene            complement(1567..1836)
                     /locus_tag="VFO72_07265"
     CDS             complement(1567..1836)
                     /locus_tag="VFO72_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479123.1"
                     /translation="MEIDDEAKEIVREIGSAINESVENSTKVAEAIERLREAGYEMEL
                     TLRLEIGLRPHAGDEDNESEAANLNLTEEDLQVLQRMKIRIDPEQ"
     gene            2004..3116
                     /locus_tag="VFO72_07270"
     CDS             2004..3116
                     /locus_tag="VFO72_07270"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9479124.1"
                     /translation="MYGTIGILTGGGDAPGLNAVIRAVVKTATCEYGMRVIGIEDGFE
                     GLLGATKTRVLSPGEVRGLLPRGGTILGTRNRGFFVERARDGGKRSVEAVYEEAIANM
                     RVLGIDALVVLGGEGTLTIANEFNKRGIPVCGVPKTIDNDLACTELTFGFVTALDIAT
                     EALDRLHTTAESHDRVMILEVMGRHAGWIALHSGLAGGADVILIPEIPFSLQAVADKI
                     RARDLAGSNFSIIVVAEGAIEIGHDLVYQDQGDELHAPRLGGIGQHLQRDLEKVTGRE
                     TRCVILGHLQRGGRPNAFDRMLATNFGACAVRALANGERGVMVALQAGDVVSVPLTEA
                     IKNIKTVPPNGQLVRTARDTGISFGAPNEATYHKPL"
     gene            3130..3612
                     /gene="greA"
                     /locus_tag="VFO72_07275"
     CDS             3130..3612
                     /gene="greA"
                     /locus_tag="VFO72_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9479125.1"
                     /translation="MSQGIKAKLQSELDELETELRIHLPKEIKRALEFGDLRENSEYR
                     AALDRQNVVKARIVELRQRISEIASIDPNKVPRDRASYGSTLVLYDAEKDEEVTYRLV
                     TPEESDPAQGLISTTSPVGRSLMGKQEGDEVGVRTPAGPRTFEIRQLRTLHDDLGNEI
                     "
     gene            3803..4528
                     /locus_tag="VFO72_07280"
     CDS             3803..4528
                     /locus_tag="VFO72_07280"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9479126.1"
                     /translation="MFGSSIGRGAQRIIDAMVRWLAMGHINPNILTVIGVALNVGCGL
                     LFGLGEFFAAGIALIVANLFDMLDGQVARLSGRVTRFGGFLDSSLDRLSDMVVFVGLM
                     VFYARDTPSHSTLNVFLAGAGLMGSVMVSYASARAESLIPKCDVGFLRRPERVVLFII
                     GALSTYPGSNNYFTDRMPAVLWVLAVGSYWTFAHRMYHTWRELNRVEGLSIADCQVPI
                     DKSKSEIGNRQLEIPKRSNEVTT"
     gene            4660..5436
                     /locus_tag="VFO72_07285"
     CDS             4660..5436
                     /locus_tag="VFO72_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479127.1"
                     /translation="MLSLTGWLIVLVFLVQTIVSMVQRGTDWFGIWSWIAAAESAAWR
                     LKWISIPVMFVAIWFGRKLYRSIRQQPERFCGLSYARAGLVASASVACLIALLIGVTV
                     PARLRQRELAKEAAIRAAWHTFEVAAIEYQRRYQTYPADFTIMRQRIPDPYGTLAEAL
                     NNLDPKGYRPYGDVAAMGAEKSHTLRGAVIRQASLSTASDDSATTGLAFTKFELRISG
                     EDKILGNEDDWIVIEGVLRRYSDVAKGGAGQSVSAGALQQ"
     gene            5443..5730
                     /locus_tag="VFO72_07290"
     CDS             5443..5730
                     /locus_tag="VFO72_07290"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET9479128.1"
                     /translation="MSFLDRFRRKTEDEASRIARLAKTGRMTDGNIIDAVSDRDGKII
                     QVTYTYMLAGVLYESSQTLTEQQQQHDADYAPGKQIVIRYDPRQPANSIVV"
BASE COUNT         1268 a         1708 c         1613 g         1311 t
ORIGIN      
        1 atgtgactga acccgtcttg gtagacgtga tccgctcctg catttcgccc atctccgtcg
       61 cgaggttcgg ctggtaacct acggcggaag gcatgcgacc gagaagcgcc gacacttccg
      121 aacccgcctg ggtaaaccgg aagatgttgt cgataaagag cagcacgtcc attccctgat
      181 cgcggaagta ctctgcgacc gtcagacccg acaacgcgac gcgaagacgc gcgccgggag
      241 gctcagtcat ctgtccatag ataagcgcta ctttcgactg gccgatcgtg ttcatgtcta
      301 ctttcgacag gtcaaacttg cccgtgtcgt ggaagttctt gttgaacgcg tcgccaaact
      361 ggataacctg cgattcgacc atttcgcgca acaggtcgtt tccttcacgc gttcgctcgc
      421 cgacgccggc gaacaccgag aaacccgagc gcgctttcgc gatgttgttg atcaactcca
      481 tcacgataac ggtcttgccc acgcccgcac cgccaaacag tccgatcttg ccgccgcgca
      541 agaacggttg aatgaggtcg atgaccttga ttcctgtttc aaacatctgc aactcagtcg
      601 actgctcttc gaaggtcggc gcatgacgat ggattggata acgcttctcg tgcttcaccg
      661 gaccgagcgt atcgactggt tctccaatca cgttcagcac gcggccgagc gtgccttcac
      721 caaccggcat cgtgatcgga ccgccggtat cgatcgcctt catcccgcgc accatgccgt
      781 ccgtcggcaa catcgaaact gtgcgcacgc ggccttcgcc aaggtgctgc tgcacttccg
      841 caatcacatc gataggcgtc ggcacgtcga atccctcaga gacgacgcgc agcgcctggt
      901 aaatcggcgg gaggtatcct ccctgaaact ctacgtcgag caccggacca atgatctgca
      961 cgaccttgcc gacctgttgt ccgtcggtgg tcacctttcc atcaggcgta gacatactgt
     1021 ctttaaactc ctctttcgac ctataaaggc aaatgaaact ggcaggtttt taaaagccag
     1081 acaagataac attgccgatt gacgattgtc gagattggcg cattggtggg tagtgtccta
     1141 atctgaacgc cagaggcgtt cgctaattcc agccagggtt gcttcaaccc tgggtcgaac
     1201 gttagcgtcc ggtcataacg ctgaagggcg ttggcaaatt cgcggttagc caacgctttc
     1261 agcgtaaaag gtaaaaacat ccaatcagtc cccagggttg tcaaaagctc caacctctgg
     1321 gctggaattc gataacgcct tcggcgttca gattaggaca ccaccgcatt ggtgattttt
     1381 gattgccgat tgaagtcatt gccgattgcc gattgccaat tgacgattta tggctgctca
     1441 atgcgggaag gtacaaatcg gcaattggca atcgaaaatc cgcaatcggc aatcgaaaat
     1501 ccgcaatcgg caatcaaaaa tccgcaattg gcaatcgaaa atcggcaatg gacaagtcgg
     1561 caatgactat tgctccggat cgattctgat cttcattcgc tgtagcactt gcaggtcttc
     1621 ttcagtgaga ttgaggttgg cggcttcgct ctcgttgtcc tcatcgcccg cgtgaggccg
     1681 caacccgatc tcgagtcgca gcgtcaactc catctcgtaa cccgcttccc gcaggcgctc
     1741 gatcgcctca gccactttcg tcgaattctc gactgattcg ttgatcgcgc taccaatctc
     1801 gcgaactatc tcttttgctt cgtcatcgat ttccaaagtg ctcgtatgct acgccactca
     1861 ttgcggattg ccaacctctg tcattgccaa ttgaagtcat tgccgattgc cgattgccaa
     1921 ttgtcaattt atttggctct tcgatcccga gcgcaaatcg gcaatcggca atcggcaatc
     1981 ggcaatggta gactcttgca tacatgtacg gaaccatcgg catactaacc gggggcggcg
     2041 atgcgccggg tctcaacgcc gtcattcgcg ccgtcgtcaa gactgccacc tgcgagtacg
     2101 gcatgcgcgt aatcggcatc gaagacggct tcgagggtct gcttggcgcc accaagactc
     2161 gcgtcctgtc tccaggtgag gtacgtggac tgctgcctcg cggcggcacg atcctgggca
     2221 cacgtaaccg cggcttcttt gtggaaagag cgagagatgg cggcaaacgc agtgtcgaag
     2281 ccgtttacga agaagccatc gccaacatgc gcgtgctcgg tatcgacgcg ctcgtcgttc
     2341 tggggggcga aggcacgctc actattgcga acgagtttaa taagcgcggc attccggtct
     2401 gtggcgtccc caaaacgatc gataacgacc tcgcctgcac ggagctgacg ttcggatttg
     2461 tcaccgcact cgatatcgcc accgaagcct tggaccgtct gcacacgacc gccgagtcgc
     2521 acgatcgtgt catgattctc gaagtcatgg gccgtcatgc cggttggatc gcattgcact
     2581 ccggccttgc cggcggcgcg gacgtcatcc ttattcccga gattccgttt tcactgcaag
     2641 ccgtggctga caagatccgt gcgcgcgatc tcgccggatc gaatttcagt atcatcgtcg
     2701 tcgccgaagg cgcgatcgag atcggccacg atctggtcta tcaggaccag ggcgacgagc
     2761 ttcacgcgcc gcgactcggc ggcatcggtc aacacttaca acgcgacctg gaaaaagtca
     2821 ccggaagaga aactcgttgc gtcatcctgg gccatctcca acgcggtgga cgaccgaacg
     2881 ctttcgatcg catgttagcg acaaacttcg gcgcgtgtgc ggtccgcgca ttggcaaacg
     2941 gcgaacgcgg cgtgatggtc gcgttgcagg ccggcgatgt ggtgagcgtt ccgcttaccg
     3001 aagcaatcaa gaacatcaag actgtgcccc caaatggcca gctcgtacgg accgcgcgtg
     3061 atacaggaat ttcctttggc gcgcctaacg aagccactta tcataagccg ctgtagagag
     3121 gttgaatcgt tgtcacaggg aatcaaagca aaactccagt cggaactcga cgaactggaa
     3181 acagaactac gcatccactt gccgaaagag atcaagcgcg cgctcgagtt tggcgacctg
     3241 cgcgagaact cggaatatcg cgccgcgttg gaccgtcaga acgtcgtcaa ggcgcgcatc
     3301 gttgaactgc gccagagaat cagcgagatt gcttcgatcg atccgaacaa agtaccgcgc
     3361 gaccgggctt cttatggttc gactctcgtc ctctacgacg cggagaaaga cgaggaagta
     3421 acgtaccggc tggtaacgcc cgaagagagc gatccggcgc aggggctaat ctcgaccacg
     3481 tcgccggtcg gacgcagttt gatgggtaaa caagaggggg acgaggtcgg agtgcgaacg
     3541 cccgccggcc ctcggacgtt tgaaatccgg cagttacgga cgctgcatga cgaccttggt
     3601 aacgaaattt gacaatctac cttgctgcgg atcgcaggcg tctcgcgtgc aattgtcggt
     3661 aggcgccgcc tccgcttgcg ggcagacgag acgcctgcgg tcccaggtga aaaaggggta
     3721 acgtttctgt gtagcctgtg gacttccggc ggttttaggg cataatcaca tcccgcgagc
     3781 gaggagaaac gtaacctaac aaatgttcgg gtcaagcatc ggacggggag cgcagcgaat
     3841 tatcgatgcc atggtgcgct ggctcgccat gggtcacata aaccccaaca tactgaccgt
     3901 tatcggcgtg gccttaaacg tcggttgcgg tcttcttttt ggtctggggg aattcttcgc
     3961 ggccggcata gccctgattg tcgcgaacct attcgacatg ctggacggcc aggtggcgcg
     4021 tctttccggc cgggtcacgc gctttggtgg gtttctcgac tcgtcgttgg accggctttc
     4081 tgacatggtt gtttttgtgg gcctaatggt cttttacgcc cgcgacaccc cgtctcattc
     4141 aactcttaac gtttttcttg cgggcgccgg actaatgggc tcggttatgg taagctacgc
     4201 gagcgcgcgg gcggagagcc tgattcccaa gtgtgatgta ggctttctgc gtcgcccaga
     4261 acgcgttgta ctctttatta tcggagcgct cagcacttat cccggttcta acaattactt
     4321 caccgatcgg atgcccgccg ttctatgggt tcttgcggtt ggctcctatt ggaccttcgc
     4381 ccaccgcatg tatcacacgt ggcgcgagct gaaccgggtt gagggattgt caattgccga
     4441 ttgccaagtg ccgattgata aaagcaaatc ggaaatcgga aatcggcaat tggaaatacc
     4501 aaaacgctcc aacgaggtaa ccacttgact atctgtccct gctgcggctt caaatttcac
     4561 ggagcactga gcagcggttg taaacagtgc ggcgcacgtg ccgttggcga gccacttccc
     4621 aggcccgccc acgaacttcc ttcttatggc cgcgcgttag tgctttccct cacgggctgg
     4681 ctcatcgtcc tcgtcttttt ggtgcaaaca atcgtgtcca tggtccagcg cggaactgac
     4741 tggttcggga tctggtcctg gatcgctgcc gccgaaagcg ccgcgtggcg actaaagtgg
     4801 atctcgattc cggtgatgtt cgtggcaatc tggtttggcc gaaaactcta ccggtctatt
     4861 cgccagcaac cggaaagatt ttgcgggctg agctacgcgc gcgccggcct ggttgcttca
     4921 gcaagcgtcg cctgcttgat tgcgttgttg atcggcgtta cggttccggc gcgcttgcgc
     4981 caacgcgagc tggccaaaga agcagcgatt cgcgcggcct ggcacacctt cgaagtcgca
     5041 gcgatcgaat atcaacgccg ttaccaaacc tatccagcag atttcacgat tatgcgccag
     5101 cgcatccccg atccttacgg cacgcttgct gaggcgctga ataacctgga tccaaagggc
     5161 tatcggccgt acggcgatgt agcggctatg ggcgccgaaa aatcccacac gctgcgtggc
     5221 gccgtcattc gccaggcttc tctctccacc gcctccgacg attcggcgac caccggactg
     5281 gcgtttacta agtttgagct ccgcatttcg ggtgaagaca agattctcgg taacgaggac
     5341 gattggatcg taatcgaagg cgtgctcagg cgatactcgg atgttgccaa aggcggcgcc
     5401 ggacaatcag tttcagccgg agctcttcaa cagtagcgac gaatgtcgtt tctcgatcgt
     5461 ttccgcagaa aaaccgaaga tgaagcctca cgcatcgctc gtctcgcaaa aaccggccgc
     5521 atgaccgacg gcaacatcat cgacgccgtg tccgaccgtg acggcaagat catccaggtc
     5581 acctatacct acatgttggc aggcgtgctt tacgagtcgt cgcaaacact cactgaacaa
     5641 caacaacagc acgacgctga ctacgcaccc ggcaaacaga tcgttattcg ctacgatcct
     5701 cgccagcccg ctaattcaat tgtcgtttga gctttgttct ttgtgctttg ttctttgtag
     5761 ttccgctagc gtagactctg agttgaacta gcaaagtaca aagctcaaag aacaaagctc
     5821 caaacaaaat gttccgtaag gtcctgatcg ccaatcgcgg cgagatcgcc gttcgcatca
     5881 ttcgcgcctg tcgcgagttg
//
LOCUS       DASTEY010000411         6683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1211918, whole genome shotgun sequence.
ACCESSION   DASTEY010000411 DASTEY010000000
VERSION     DASTEY010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211918"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFO72_07295"
     CDS             <1..267
                     /locus_tag="VFO72_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9479129.1"
                     /translation="SILKKKRQHVTSYTVEVSGERRDEYPRSYTSMKVHHILTGRAIS
                     AKAVAQAIELSETKYCSVADSLRPKVEIHSTFEIIEEPHEISGE"
     gene            371..1681
                     /locus_tag="VFO72_07300"
     CDS             371..1681
                     /locus_tag="VFO72_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9479130.1"
                     /translation="MRRSDAAAGGGNVPRLTPAEHMRRAAIYLRNRAFAEARGHWQAL
                     LANHPQDPRAPEALLGIGRSYFLSKVYQDGYVVFNELVQKYPATKEGREALNYSAASL
                     LRMGKFSESAARYEEYINRFPDGERIDTAHLNMIDTLREANRPADALVWVTRTRQRFA
                     GSATETNAMFAALRLHVAEREWSKAVVMADQLSQRTFAKAVQTSRAEVAYLKAFSLEQ
                     AGRRHEAFSAYLAVPDGIDSYYGWLASQRLAALADKERAHIVIERENRVNSAIAKVAD
                     SYPASYRQAILSAAKVRRIDPRFILALIKQESVFKPFAKSPAGARGLLQLTVDTAKKY
                     APAAGINSVRESQLYKPETSIMVGAEYLEHLMKLFPQMLEPVAASYNGGEDNVARWLE
                     RAKHKDRGVFTAEIGFDETKGYVQKVMSNYRVYRQLYTVDLIRK"
     gene            complement(1700..2449)
                     /locus_tag="VFO72_07305"
     CDS             complement(1700..2449)
                     /locus_tag="VFO72_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479131.1"
                     /translation="MSKLRWAIACGAFLLTTFVGIGFVLLQNSAAAAQESQTERVVKG
                     DHRRLPVFQIEVLVDGRPLAPRYERGRLYVNAVQGAEYELRLRNSSTERVAVALSVDG
                     LNTIDARQTSAWNASKWVIEPHQTITISGWQMSSERARRFYFTNEHDSYVRKLGRKAN
                     IGVISAVFFRERRRVMPVTKGPVTQENSPAPAESQLRAEASRDSRAATGIGRSVRNEV
                     SWIDMELDPKAVAELIVRYEYSDSRYCPEPK"
     gene            2669..3319
                     /locus_tag="VFO72_07310"
     CDS             2669..3319
                     /locus_tag="VFO72_07310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9479132.1"
                     /translation="MEFALTATKNKARAGTYYDRIARFYDLTFKLNGYGRALDQYLET
                     HPLPISRGARILDAGCGTGLLTHALLRAIRFPVAITALDLSSTSVVEARKSLYYSAGR
                     KRDVTFAQGNLLCLPFADESLDLVVTSGALEYVKLSDGLTELARVIAPGGHLLHLPVH
                     PSVIGVVLEILFRFKSHPPQEVKDETEKHFRIIHQYRFPRQEPIGWSKTAILAQKV"
     gene            complement(3424..4572)
                     /locus_tag="VFO72_07315"
     CDS             complement(3424..4572)
                     /locus_tag="VFO72_07315"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9479133.1"
                     /translation="MSSAFSSILIALAIAFCVGCDRAPEGRGAGEVESSPQSNVSFKI
                     EKVAGGLQVPWSIVWAPDGRMIFTERPGRVRVIENGTLRPQPLFVVPDVESSGESGLM
                     GIALHPQFSSNHFVYLAYAYSSGGSRVRVVRYREAPEGLVDRTVIIEDIPSAQYHAGC
                     RIAFGPDGKLYVTTGDATRRELAQRLDSLAGKTLRLNDDGTVPQDNPFVGQQNARPEI
                     WSYGHRNAQGMDWQPGSGLIFQTEHGPSGFDGPEGGDEVNIVERGKNYGWPLVHHKEN
                     RSGLEPPLLEYTPACAPASGMFYSATVFPQFKENFFFGCLRGERLIRVELDGRRVVRQ
                     EDLVKDYGRIREVAQGPDGYLYFSTSNRDGRGKPASDDDRILRLVPAQ"
     gene            complement(4586..5008)
                     /locus_tag="VFO72_07320"
     CDS             complement(4586..5008)
                     /locus_tag="VFO72_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9479134.1"
                     /translation="MLIRDVIRNREPYSTKSSATVQEAAEFMAARNIGAVCVIDDEGR
                     LVGVFSERDVLNRIVVPHRDPQTVKVGEVTSELRAVIQCDETPHQALERMELIGTRHL
                     PVVDGERWVGMLSMRDLLRVEVGEQGDEIKLLHEYIQH"
     gene            complement(5142..6158)
                     /locus_tag="VFO72_07325"
     CDS             complement(5142..6158)
                     /locus_tag="VFO72_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9479135.1"
                     /translation="MQYVANPPNPWLTHSVEWLGEPPETKLEVFEETETRHIITHNDS
                     PDVGFDYTVNCYRGCIHGCTYCFSRPTHEYLGYGAGTDFDRKIVVKVNAPKLLREELM
                     KPSWKGDEIVFSFTSDPYIPLEANYKLTRRCLEVCAEFQQPIGVITKSAMIRRDIDVL
                     QELSRKAHLGVFLTIPFTDVEAARAVEPYAPLPEARFHAMEALAAAGITVGIGIAPVI
                     PGLTTDIPVLLKRAKEAGAQHAFINMLRLPGSVAPYFEERLREKLPTKADRVLNRVRE
                     ARDGKLNSSVFGERMRGKGEYWAATERLFKIHCQRLGFNKKRTHETSQTTTFRRPSAQ
                     ASLF"
     gene            complement(6162..6386)
                     /locus_tag="VFO72_07330"
     CDS             complement(6162..6386)
                     /locus_tag="VFO72_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479136.1"
                     /translation="MAGQHVENLFRRFKGEKISVKTISGGIYEGRVVEITNDYVCLSE
                     TPGTESQPVFIFFTAIESMAATVAMNDFQG"
     gene            complement(6390..>6683)
                     /locus_tag="VFO72_07335"
     CDS             complement(6390..>6683)
                     /locus_tag="VFO72_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9479137.1"
                     /translation="EPPMARLRGGELKITLAQHLETMLRRGRGVHFFFGVEDVDRFYD
                     QLVERGADVKPPLDEGWGGRFITLEDPDKYRFFFVTWTTAEQEKLLAQAKETG"
BASE COUNT         1450 a         1929 c         1828 g         1476 t
ORIGIN      
        1 tcaattctga agaagaagcg gcaacacgtg acgagctaca cggtagaagt ttcaggcgaa
       61 cgccgcgacg agtacccgcg aagctacacg tcgatgaagg tccatcacat tcttaccgga
      121 cgagcaatct cagcgaaagc tgtggcccag gcgatcgaac tttccgaaac gaaatattgc
      181 agcgttgccg actcgctaag gccgaaggta gaaatccatt ctacgtttga gatcatcgaa
      241 gaaccgcatg agatatccgg ggagtaatca agtaaaagtg ttaaaaaaga ttgccgccgc
      301 ccttagtctt tctctcctgt taacgaatca agctttttcc cagtcagcca gtgaaactgc
      361 cctcgcggcc gtgcgccgga gcgatgccgc ggccggcggt ggaaacgttc ccaggctcac
      421 gccggccgaa cacatgcggc gcgccgcaat ctacttgcgc aatcgagctt tcgcggaagc
      481 acgcggccac tggcaggcgt tactcgcaaa ccatccgcag gatccgcgcg ctcccgaagc
      541 gctgctcggt atcggccgtt cgtatttcct tagcaaggtt tatcaggacg gctacgtcgt
      601 cttcaatgag ttggtccaga agtatccggc cacaaaagaa ggccgggagg ctctgaacta
      661 cagtgccgct tcactgttac gcatgggcaa gttctccgag tctgccgcac gttacgagga
      721 atatatcaat cgcttccccg acggcgaacg catcgacacc gcgcacctga acatgatcga
      781 cacgttgcgc gaagcgaatc gtccggcgga cgcgctcgtt tgggtaacac gcactcgtca
      841 gagattcgcc ggcagtgcga ctgaaactaa tgccatgttt gcggcacttc gtctgcacgt
      901 cgccgaacgc gaatggtcca aagcagttgt catggccgac cagttgtcgc aacgaacgtt
      961 tgcaaaggca gtgcaaacgt cgcgtgcgga agtcgcatat ctcaaggcct tcagtctcga
     1021 gcaggcaggc cgcagacacg aggcgttctc tgcttacctg gcggttcctg atggaattga
     1081 ctcgtactac ggctggctcg cgagtcagcg gctcgccgca ctggcggata aagagcgcgc
     1141 gcacatcgtg atcgaacgcg agaatcgagt gaactcggcg atcgcgaaag ttgctgattc
     1201 gtatcccgcg tcataccgtc aggccatatt gagtgcggcg aaagtgcgac gcatcgatcc
     1261 gcgttttatt ctggccctga ttaaacagga gagcgtgttc aaacccttcg ccaaatcgcc
     1321 ggctggagcg cgcgggctgt tgcagctcac tgttgacacg gcgaaaaagt acgcgcctgc
     1381 ggcaggcata aattcagtgc gtgagagtca actttacaaa cccgaaactt caatcatggt
     1441 gggcgccgaa tatctcgaac acctgatgaa attgtttccg caaatgctcg agccggtcgc
     1501 tgcgtcctac aacggcggtg aagataacgt tgcccgctgg ctcgaacgcg cgaaacacaa
     1561 ggaccgcgga gttttcacgg cggagatcgg gttcgacgaa actaagggct acgtgcagaa
     1621 agtgatgtca aactaccgcg tctatcgcca actctacacc gtcgatctga tccgtaaata
     1681 atccgtgaga ttccgtggcc tacttcggct ccgggcaata ccggctgtcc gaatattcat
     1741 acctcacgat caattccgca acggccttgg gatcgagttc catatcgatc caactgactt
     1801 catttctaac ggagcgtccg attcctgtcg ccgcacgact gtcgcgcgat gcttcggctc
     1861 gtaactgcga ctctgccggc gccggcgaat tctcctgcgt aaccggccct ttcgtaaccg
     1921 gcataacgcg ccttctctcc ctgaaaaaca ctgccgagat cacaccaatg tttgctttgc
     1981 ggccgagttt ccgcacatac gaatcgtgct cattggtgaa atagaaacgc ctggcgcgct
     2041 ccgaactcat ctgccaaccg ctaattgtga tcgtctggtg cggttcgatt acccacttgc
     2101 tggcgttcca ggctgaagtc tgccgtgcat cgattgtgtt caagccatcg actgacaggg
     2161 ccaccgccac gcgctccgtc gaagagttgc gcagacgaag ttcgtactcg gctccttgca
     2221 ccgcattgac atacagccgt ccacgttcgt accgcggggc cagcggccgc ccatccacga
     2281 gcacctcgat ttgaaaaaca ggaagccggc gatgatcgcc tttgacgacg cgttctgttt
     2341 gtgactcctg tgcggctgcg gcgctgtttt ggaggagaac gaacccaatt ccgacaaatg
     2401 tagtcaaaag aaacgcgccg caggcgattg cccaacgaag tttgctcatg aaccttgttc
     2461 ctcctgtaag aattcgcttc aaaccgaaga acggacgggc ggccgggttc ttgcaaggta
     2521 cgtatgaagg ccgtacgtgt ttgtcagtgc attggctttt cggttgagta atattggcga
     2581 ggaggctaca atggcccgcg ccacgccggg atgaaataaa ctgaaacatc gcaatacgta
     2641 accgaggggt gaggcttaag agaagctcat ggagtttgcc ctaactgcaa ccaaaaacaa
     2701 ggcgcgcgcg ggcacttact atgaccgaat cgcccggttt tatgacttga ctttcaagtt
     2761 gaacggttac ggtcgcgcgt tggatcaata cctcgagact catcccctgc ccatttcgcg
     2821 tggcgcccgc attctcgatg ccggttgcgg cacgggactc ctgacgcatg ctttgctgcg
     2881 cgcgatccgg tttccggtcg cgattacggc gctcgatctt tcctccactt cagtcgtcga
     2941 ggcgcgcaag tcgctctatt acagcgccgg aagaaaacgc gatgtaactt tcgcacaggg
     3001 caacctgctg tgtttgccgt tcgccgacga gtcgctcgat ctggttgtga cgtcgggagc
     3061 gttggaatac gtgaagctct ccgacggact cacagaactc gctcgcgtga tcgcacccgg
     3121 cggtcacttg ctacatcttc cagttcatcc gagtgtgatc ggcgtagtgc tcgaaatcct
     3181 gtttcggttc aagagccatc cgccgcagga agtgaaggac gagacggaaa aacacttccg
     3241 catcattcat caataccgtt tcccacgaca ggaaccaatc ggctggagca agaccgcgat
     3301 tctcgcgcag aaggtctaat cgatgtaatc gccttctttg tgtctctgtg cctctgtggt
     3361 taactaccac cgagacacaa aggcacagaa aaatcagact ttcaggcgaa gcctgtctaa
     3421 tctttattgc gccggcacca acctgaggat gcgatcgtca tcggaagccg gcttgccgcg
     3481 accatcgcgg tttgaagtcg aaaaatacag atagccgtct ggtccttgag caacttcacg
     3541 aatgcgacca tagtctttga cgaggtcttc ctgccttact acgcgcctgc catccaactc
     3601 gacgcggatc agtctctcgc cacgcaggca accgaaaaag aagttttctt tgaactgtgg
     3661 gaacaccgta gcactgtaga acatgccgct cgcgggtgcg caggcgggag tgtattcgag
     3721 caacggcggc tccaaacccg atcgattctc tttgtgatgg accagtggcc agccatagtt
     3781 tttgcctctc tcgacaatat tgacttcgtc tccaccttcc ggaccatcga aaccgcttgg
     3841 accatgctct gtttgaaaga tcagaccgct gcccggctgc cagtccattc cctgcgcgtt
     3901 gcgatgaccg taggaccaga tctcgggccg cgcattttgt tgtccaacga atggattgtc
     3961 ttgcggaacg gtgccgtcgt cgttcaaacg cagtgttttt ccggcgagcg agtccaggcg
     4021 ttgggccagc tcgcgccgcg tggcgtcgcc ggtcgtaacg tagagcttgc cgtctggacc
     4081 aaacgcaatc cggcagccgg cgtgatattg cgcggaagga atgtcttcaa tgataacggt
     4141 gcggtcgacc aggccttccg gcgcttcacg gtagcgcaca acacgaacgc gggaaccacc
     4201 gctcgagtac gcatacgcga ggtagacgaa atgattgctg ctgaactgcg gatgcagtgc
     4261 gatacccatc aaaccacttt cacctgacga ctcgacgtcc ggcacaacaa acaacggctg
     4321 tggccgcaaa gttccgtttt caatgactcg cactcgtccc ggacgctccg tgaaaatcat
     4381 gcgaccgtca ggcgcccaga cgatggacca cggcacctgt agtccaccgg caaccttttc
     4441 aattttgaaa ctgacatttg actgtggtga agattcaact tcgccggcgc cgcgtccttc
     4501 cggcgcgcgg tcacagccaa cgcagaacgc gattgccaga gcgatgagaa tactcgagaa
     4561 ggcggatgac acgtagatgc tggacctagt gctgaatgta ctcgtgaagc agcttgatct
     4621 cgtctccctg ctcgccgacc tcgacgcgca acaggtcgcg catcgagagc atgcccaccc
     4681 agcgttcgcc atcgacgacc ggcaggtgac gagtaccaat taactccata cgttcgaggg
     4741 cttgatgggg agtctcgtca cattggatca cagcgcgtaa ctcgctcgtt acttctccga
     4801 ccttcacggt ttgcgggtcg cggtgcggaa ccacgatgcg attcaaaacg tcgcgctcgg
     4861 aaaacacgcc gaccagtctt ccctcgtcat cgattacaca gaccgcgcca atgtttcgag
     4921 cagccataaa ctccgcggcc tcctgcacgg tggcagaaga tttcgtcgag taaggctcgc
     4981 ggttgcgtat cacatcgcgg atcagcatcg catttgcctc ctgatttggc tgtggggtga
     5041 ccggtattat aaagaatgag gcggcgtgtg tctctagttt tgatcttggc actttgttct
     5101 ttgttctttg caaggtcaaa gtacaaggct ctaagttcga attaaaacag gctggcctgt
     5161 gcggacggac ggcggaaggt cgttgtttga gacgtctcgt gggtccgttt cttgttgaag
     5221 cccagacgct ggcagtgaat cttgaaaagg cgttctgtgg cggcccagta ctcgcctttt
     5281 ccgcgcattc tttcgccgaa cacgctcgag tttaacttgc cgtcacgcgc ttcacgtacc
     5341 ctgttgagca cgcgatcggc cttcgtcggc aacttctcgc gcaaccgttc ttcgaagtac
     5401 ggcgcgacgc tgccgggcaa acgcaacatg ttgatgaaag catgttgtgc gcccgcttct
     5461 tttgcacgtt tgagcaagac gggaatgtca gtcgtcaacc ccgggatgac cggcgcgatg
     5521 ccgatgccga ccgtgatgcc tgcggctgcg agtgcctcca tcgcgtgaaa acgcgcttcc
     5581 ggtaagggcg cgtaaggttc taccgcccgc gccgcctcaa catccgtgaa cggaatcgtg
     5641 agaaacacac cgaggtgcgc cttgcgcgac agctcctgca acacgtcgat gtcgcgcctg
     5701 atcattgctg acttcgtgat gacgccgatc ggttgctgaa actccgcgca cacttcgaga
     5761 cagcgtcgcg tgagtttgta gtttgcttcc agcggtatgt acggatcgga agtgaacgag
     5821 aacacgatct cgtcgccttt ccacgacggc ttcatcaact cctcgcgcag cagtttgggt
     5881 gcatttacct tgacgacgat cttgcgatcg aaatctgtgc ctgcgccgta tccgagatat
     5941 tcgtgcgtgg gccggctgaa gcagtaagtg cagccgtgga tacagccgcg gtagcagttg
     6001 acggtgtagt cgaaaccaac gtcggggctg tcgttgtgag tgatgatgtg gcgcgtctcg
     6061 gtctcttcga agacctcgag cttggtttcc ggaggttcgc cgagccattc gaccgaatgc
     6121 gttaaccagg gattgggtgg gttcgcaacg tactgcattt ttcaaccctg gaagtcattc
     6181 atcgcgacgg ttgcggccat cgactctata gccgtgaaaa agatgaagac cggttggctt
     6241 tctgttccgg gcgtttcact caggcagacg taatcgttgg tgatctcgac tacacggcct
     6301 tcgtaaatgc cgccggagat tgttttgaca ctgatcttct cgcccttgaa gcggcgaaag
     6361 agattttcta cgtgttgacc agccatcact caccccgttt cttttgcctg cgccaacagt
     6421 ttttcctgct cggccgtggt ccacgtcaca aagaagaagc ggtacttgtc cgggtcttcc
     6481 aaagtgataa accggccgcc ccaaccttca tcaagcggag gcttaacgtc agcccctctt
     6541 tcaaccagtt gatcgtagaa ccgatcgacg tcctcgacgc cgaagaagaa gtgcactccg
     6601 cgtccgcgcc gcaacattgt ctccagatgt tgggccagcg tgattttcag ctcgccacca
     6661 cgaagtcggg ccatcggcgg ctc
//
LOCUS       DASTEY010000412         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_601972, whole genome shotgun sequence.
ACCESSION   DASTEY010000412 DASTEY010000000
VERSION     DASTEY010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601972"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..394)
                     /locus_tag="VFO72_07340"
     CDS             complement(59..394)
                     /locus_tag="VFO72_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479138.1"
                     /translation="MRLKLVLAASALAAFAGAGSCIALVLGVFSVKTLSSPGLLVAST
                     LLLPVATIVFAAIFVYRHTARRRKLQAFLTTIIATVLTLCLFALASILSARLSPTEPA
                     QPPGPHIAS"
     gene            573..1493
                     /locus_tag="VFO72_07345"
     CDS             573..1493
                     /locus_tag="VFO72_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET9479139.1"
                     /translation="MISRSALIWLSRQEGLKDFAARFRLFKKLTTRFVAGETIDEAVS
                     HIRAINAENCTASFDHLNESVATESEAEAEVVEYLNILRRISETGIRSNVSIKLTQFG
                     LNLDPELAYNNARRVVEEAHARGNFVRVDMEDSSVTQATIDLFKRLRAEFDLNTVGIV
                     LQSYLRRTYADAQELVKLPARIRICKGAYDEPPEVAFPDKSEVDANYVKVMQLLLSSG
                     TYHGIATHDPKMIDATIDFASREGIGKEKFEFQMLFGIRRDLQRQLARDGFNMRVYVP
                     YGKHWYPYFMRRLAERPANVWFVLKNVLKG"
     gene            1565..2005
                     /locus_tag="VFO72_07350"
     CDS             1565..2005
                     /locus_tag="VFO72_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET9479140.1"
                     /translation="MAEIFTSGFVLLWFGIGALAAAFVGFIGVDSLVIQFMVFAIVSI
                     ALTAASRTIFVNYFSREKTGQSLRSGVDALPGKIGTVVSSSKGALQEGAVKVFGSTWT
                     AYPAAGEPPLEAGERVCVESVEGASIYVRRVDGDPEWRQKALPE"
     gene            complement(2008..>2915)
                     /locus_tag="VFO72_07355"
     CDS             complement(2008..>2915)
                     /locus_tag="VFO72_07355"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET9479141.1"
                     /translation="YAKSDVGRVRRGNEDNFLVLDLSSEQTWTGADGATPPQKLTRFT
                     VGQRGLVLVVSDGMGGALAGDVASRMAVDSVREMLLGNEGEACDPQLDLVECLKNATI
                     YANLAIHLKSQEDSRCAGMGATFTGAAVRGDNLDLVQVGDSRGYVIRKDHIRLATKDQ
                     SLVQQLVDVGQISESEAETHMFRNVILQALGAQSEVTPVTGRIRLRQGDVLLLCSDGL
                     SGKLRAEDIQQIVVSQNDLARACEALIDEANNRGGEDNITVVLARFRGDDLEEPATDR
                     ITIELPPLEEDKTLDDNFEADTEPH"
BASE COUNT          650 a          771 c          842 g          652 t
ORIGIN      
        1 ctttgcgtcc ggtccaacgt gagatcggtc tcaacgtcgc tgaagcaagg gcgagcggtt
       61 aagaagcgat gtgtggtcca ggcggttgcg ccggctcagt cggcgacagc cgcgcactca
      121 agattgaagc aagggcaaaa aggcagagcg tcagcacggt ggcaatgatg gtcgtcaaaa
      181 aggcttgtag cttgcggcgg cgcgcggtgt gacgataaac gaagatggct gcgaaaacga
      241 tggtcgcgac gggcagtaac aacgtcgacg cgacgagtag accagggctc gacaaggtct
      301 tcactgagaa gacgcccaat acgagcgcga tgcaggaacc agcacctgcg aatgccgcga
      361 gcgctgaagc ggccaataca agcttcaatc gcattcgcga acgttcctca aggtggtttt
      421 gggcaaattt gaccctccct tgaattggcg aaaaaacccg ccaagaatac cacaaggcag
      481 gaccatacgc ggcgttactt ctgtgcctcc gtgcctctgt ggttatagat taaccactga
      541 gacacaaaga cacagagaaa aagaaaacag ggatgatctc cagaagcgcg ctgatttggc
      601 tgtcacgaca ggaaggcctc aaagattttg ccgctcgttt tagactcttc aaaaaactta
      661 ccacacgctt cgtagcgggc gagaccattg acgaagcggt ttctcatatt cgcgcaatca
      721 acgcggaaaa ctgcaccgct tcgttcgatc atttgaacga gtcggtggcg actgaatcag
      781 aagccgaagc ggaagtcgtc gagtatctca atatactcag gcgaataagt gagacgggaa
      841 ttcgctcgaa cgtctcgatc aagttgacgc agtttggcct caatctcgat ccggagctgg
      901 cctacaacaa cgcgcggcgt gtagtggaag aagcgcacgc tcggggcaac ttcgtccgcg
      961 tcgatatgga agactccagc gttactcaag ccaccatcga tcttttcaaa cggctgcgtg
     1021 cggaatttga tttgaatacc gtcggcattg ttttgcagtc gtacttgcgg agaacttatg
     1081 cggacgcgca ggaattagta aaacttccgg ctcggatccg tatctgcaag ggcgcttacg
     1141 acgagccacc cgaagtagcc tttcccgaca agagcgaggt ggacgcaaac tacgtcaagg
     1201 tgatgcaact gttactctcg agtggcacgt atcacggcat cgccacacac gatccaaaga
     1261 tgatcgacgc caccatcgac ttcgcctccc gcgagggcat agggaaggag aagttcgagt
     1321 ttcagatgct gtttggcatt cgacgcgacc tgcaacggca gctcgcccgc gacggtttta
     1381 acatgcgagt ctatgtcccg tatggaaaac actggtatcc ttacttcatg cggcggctgg
     1441 cagagcgccc ggcaaatgtc tggtttgttc ttaagaacgt tttgaagggt tagaccacgg
     1501 ataacacgga cgtatgagcg agtggttttg ggttttgtgg gtttttttgg gtgccatatt
     1561 gatagtggcg gaaatcttca cgtctggctt tgtgctgctg tggtttggca ttggcgcgct
     1621 ggcggctgcg ttcgtgggtt ttattggtgt cgatagtctg gtcattcagt tcatggtctt
     1681 cgcaatagtt tcaattgcat tgacggcggc ctcacgcacc atcttcgtca actacttctc
     1741 gcgagagaaa acgggccagt cgctacggag cggtgttgac gcgctgcccg gaaagatcgg
     1801 cacggtcgtt tcgtcgagca agggagcgtt gcaggaaggc gcagtgaaag ttttcggttc
     1861 tacctggacc gcctatcccg ccgccggcga gccaccgttg gaagcgggcg aacgtgtttg
     1921 tgtggagagc gtggaaggtg cgtcgatcta cgtccggcgc gtggatggtg atccggagtg
     1981 gcggcagaaa gcgctgcccg agtagaacta gtgcggttcg gtgtctgctt cgaagttgtc
     2041 gtcgagagtt ttgtcttctt cgagcggtgg tagttcgatc gtgattcgat cggtagctgg
     2101 ttcttcgaga tcgtcaccgc gaaaccgcgc cagtacgacg gtgatgttgt cctcgccgcc
     2161 gcggttatta gcctcgtcaa tcaacgcctc acacgccctt gccaaatcgt tctgactgac
     2221 gacgatctgt tgaatgtctt cggcgcgcaa ttttcccgac aagccgtcgc tacaaagcaa
     2281 cagcacgtcg ccttgccgca aacgaatcct gccggttacc ggtgttactt cgctttgtgc
     2341 gccgagcgct tgcaggatca cgttacggaa catgtgcgtc tcggcttctg attcgctgat
     2401 ttgaccaacg tcgacgagtt gctgtaccag cgactgatcc ttggtggcga gacgaatgtg
     2461 gtctttgcga atgacgtagc cgcgactatc accaacctgc accaggtcca ggttgtcacc
     2521 tctgaccgca gctccggtaa acgttgcgcc catgcccgcg caacgagaat cttcctggct
     2581 cttgagatga atcgcgaggt ttgcgtagat agtcgcgttc ttcaaacact caaccagatc
     2641 caactgcgga tcgcacgcct caccttcgtt gccaagcaac atctcgcgaa cggagtcgac
     2701 cgccatgcgg ctggcgacat cgcccgcgag tgctccgccc atgccgtctg agacgacaag
     2761 cacgagtcct ctttgaccaa ctgtgaaacg cgttagcttc tgaggtggag tggcgccatc
     2821 ggccccggtc caggtctgtt cggatgagag atccagaacg aggaaattat cttcattgcc
     2881 acgtcgcacc cggccaacat ctgatttggc gtata
//
LOCUS       DASTEY010000413         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_849608, whole genome shotgun sequence.
ACCESSION   DASTEY010000413 DASTEY010000000
VERSION     DASTEY010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849608"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="VFO72_07360"
     CDS             <1..1417
                     /locus_tag="VFO72_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9479142.1"
                     /translation="GASVSPGYWTSKGRINGREGEWLHTGDVGQLDPNGNLFFKGRQK
                     DVIVGAAGLNVYPEDLEAALDRQPEIRASCVVAFEGPRGAEPFATIVPHDATADVEAA
                     VKRANEQLAEYQQIRRWSIWPEPDFPRTATHKILKREVTARLQSVSSNPPDLLTTLAH
                     VAGNLPTKLDPSTRLSDLQLDSLGRVELLSALEDRYQISLDEAAFTANTTVADVQKIL
                     RGEIEEHAAAPYPYPNWPRWRVVKLIRMFLFYSVILPVTHWMSRMSVVGRENLQRLSG
                     PALFIANHVSMGDHALILAALPFRRRHRLAIAMEGELLQGWLNPPAGTSLPLRLRWLA
                     QYVLVNLFFHVFPLPKKTGFRRSFAYAGECIDRGESVLVFPEGIRAPRGQMHMGQFKA
                     GIGVLAQALNIPIVPVKLEGLYELKHRAQYFADPGMVRVIFGEPLTVRPDSSPASITQ
                     DLERLFNTDMTDGTLTHLCSI"
     gene            1405..2688
                     /gene="serS"
                     /locus_tag="VFO72_07365"
     CDS             1405..2688
                     /gene="serS"
                     /locus_tag="VFO72_07365"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9479143.1"
                     /translation="MLDLNYVRENIDKVREALEARHADTTPLDDFARADEERRRTIAE
                     SDQLNAQRNAASREIGGLMKEGKKEEAEARRAHVGKLKERIAELDHLRAQTEARMSDL
                     LSSLPNVPHESVPRGDSETDNVEVRRWGTPPQFNFEPKDHVDLGIALGILDLERAAKI
                     SAARFAILNGAGARLERALINFMLDVQTREHGYAETLPPFIVNRAALFGTGQLPKFEA
                     DLFKLQDSRDLYLIPTAEVPVTNYHREEILDASQLPLRWAAYTPCFRSEAGSYGRDTR
                     GVIRQHQFEKVELVKYSLPENSYEELESLTRDAETILQKLGLHYRVVALSTADLGFGS
                     AKTYDLEVWLPSQNAFREISSCSNYEAFQARRAQIRFRRAGGAKVEFVHTLNGSGLAI
                     GRTWIAVLENYQQEDGSVIVPEALRPYMCGVDRIE"
BASE COUNT          614 a          863 c          782 g          580 t
ORIGIN      
        1 tggtgcaagt gtctcgccgg gctattggac cagcaaaggc cggattaatg gacgcgaagg
       61 cgagtggctg cacacgggcg acgtcggcca gctcgatcca aacggaaatc tcttcttcaa
      121 aggccggcag aaggacgtga ttgtcggtgc agccgggttg aacgtttatc cggaagacct
      181 cgaagcagcg cttgatcgcc aacctgaaat acgcgccagt tgtgtcgtag catttgaagg
      241 tccacgcggc gccgaaccgt tcgcgacgat cgtcccgcat gatgcaactg ccgatgtcga
      301 agcggcagtc aaacgtgcga acgaacaact cgccgagtat caacagatcc gccgctggtc
      361 catctggccg gaaccggact tcccacgcac cgccacgcac aagattctca agcgtgaggt
      421 cactgctcgg ctgcaatccg tctcttccaa tccaccagat cttcttacca cgctcgctca
      481 cgtcgcgggc aaccttccga ctaaactcga tccgtcgact cgcctttccg atcttcaact
      541 cgactcgctc ggtcgcgtcg aacttttgag cgcgcttgaa gatcggtacc aaatatcgct
      601 cgacgaagca gcgtttacag ccaacacaac tgttgccgac gtgcaaaaga tcctgcgtgg
      661 tgagatcgaa gagcacgctg ccgcgccgta cccttaccca aactggccac gttggcgggt
      721 ggtgaagctg atccgaatgt tcctctttta ttcggtcatt ctaccggtca ctcactggat
      781 gagccggatg tctgttgtgg ggcgtgaaaa tttgcagcgc ctctcgggac cggctctttt
      841 catcgccaat cacgtttcga tgggcgatca cgcactgata ctcgccgccc ttccatttcg
      901 ccggcggcac cgcttggcca ttgccatgga gggcgagctc ctgcagggtt ggctaaaccc
      961 gcctgcggga acgagtctgc cgctgcgttt gcgctggttg gcgcagtacg ttctcgtaaa
     1021 cctgttcttt cacgtctttc cactgccgaa aaagaccggc tttcgtcgta gctttgctta
     1081 cgcgggcgag tgcatcgatc gtggcgagag cgtactggtc tttccggaag gcatacgcgc
     1141 accgcgtggg cagatgcaca tgggccaatt caaggcgggc atcggcgtcc tggcgcaagc
     1201 cctgaatatc cccatcgtgc ccgtgaaact cgagggcctc tatgaactaa aacaccgcgc
     1261 acaatacttc gccgatcccg gcatggttcg ggtcatcttc ggcgagccgc tcaccgtccg
     1321 ccccgactcc agccccgcct ccatcaccca ggaccttgag cgcctcttca acacggatat
     1381 gacggatggt acactcacgc acctatgctc gatctgaact acgtgcgtga aaatatcgat
     1441 aaggtgcgtg aagcgctgga ggcgcggcac gcggacacca caccactcga tgacttcgcc
     1501 agggctgacg aggagcgccg ccgcaccatt gctgaatccg atcaactgaa cgcgcaacga
     1561 aacgcggcca gccgcgagat cggcgggctg atgaaggaag gtaagaaaga agaagcagaa
     1621 gcacgtcgcg cgcacgtcgg taagctcaag gagcgaatcg ccgagttgga tcacttgcga
     1681 gcccaaactg aggcacgcat gagtgatctg ctttcgtccc tgcccaacgt gcctcacgaa
     1741 agcgttccgc gcggtgacag cgagacggat aacgtcgagg tgcgtcgctg gggtacgcca
     1801 cctcagttta actttgaacc aaaagatcac gtcgacttag gaatcgcact cggcatactc
     1861 gacctggaac gcgccgcgaa gatctccgcc gcgcgtttcg cgattctgaa cggcgccggc
     1921 gcgcgactcg aacgagccct gatcaatttc atgctcgacg ttcagacgcg cgaacatggc
     1981 tacgctgaaa ctttaccgcc gttcatcgtg aatcgcgctg cactgttcgg cactggccag
     2041 ttgcctaagt tcgaagctga tcttttcaaa ctccaggaca gtcgcgatct ctacctgata
     2101 cccacggccg aagtaccggt cacgaattac catcgcgaag agatactcga cgcgagccag
     2161 ctgccgctgc gttgggccgc gtacacacca tgctttcgtt cagaagccgg cagctacgga
     2221 cgagacacgc gtggcgttat tcgccagcac cagttcgaaa aagtcgaact cgtcaaatac
     2281 tctttgccgg aaaactctta cgaggaactc gaatcgctga ctcgcgacgc ggaaacaatc
     2341 ctgcaaaaac tcgggctgca ctatcgcgtc gtcgcgctgt caactgccga cctcggtttc
     2401 ggttcagcaa agacttacga cctcgaggtc tggttgccgt cgcagaacgc ttttcgcgag
     2461 atctcatcct gctcaaacta cgaagcgttt caggcgcgcc gcgcgcagat ccgtttccgc
     2521 cgtgcaggcg gagcgaaagt cgagtttgtg cacacgctaa acggatccgg actggcgatt
     2581 ggacgtacgt ggattgcagt gctggaaaac tatcagcagg aagatggatc ggtgatcgtt
     2641 cccgaagcgc tccggccgta catgtgtggc gttgatcgga ttgagtaggc tttgcctcaa
     2701 aagtccctat ctcattctca ccgggcttca gcccggtgaa tgggcacacg attgtgagaa
     2761 accgtttcaa cggtttcgct cgcgaattca cgaaaaaccg ttgaaacggt ttcctccgtc
     2821 tccactgccc ttgcaccgg
//
LOCUS       DASTEY010000414         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_882744, whole genome shotgun sequence.
ACCESSION   DASTEY010000414 DASTEY010000000
VERSION     DASTEY010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882744"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFO72_07370"
     CDS             <1..698
                     /locus_tag="VFO72_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479144.1"
                     /translation="DPVASLNSVLPAVKAQPNRPVPELTNLTLMAARMNAPAAPAIAS
                     DTVDPDLAWAVDTSIKSWRVFKPAGENFSAEMPAGGKQVTISVPFQDQMFDFNVYVVR
                     DGFAIYSAMWITAPSDGESDKAALMVTVRGLLKGMVEGYKNEGGGPFSCELESERNVS
                     INGYTGSEFDLPACTVPGKVRVFTKVVGDQRQMYCGAVFYWQDDPNVSRFLKSFTAGP
                     TARAKRPATISER"
     gene            complement(785..1537)
                     /locus_tag="VFO72_07375"
     CDS             complement(785..1537)
                     /locus_tag="VFO72_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9479145.1"
                     /translation="MRRVFRWLFLVVLGVIVGVIAYEGILMLRVYWLRTSNPSSTSLI
                     DARAREARARGQQPRREQVWVPLQRISPNLQRAVLAGEDTNFLTHRGFDYEAIQKAFE
                     QAQREAAREAKREGENDDWLPNLPEFKRGGSTISQQLAKNLYLSSQRSFVRKGQEALV
                     TIMLERLLTKRRILEIYLNVIEWGDGIYGAEAASQRYFRKPASALNASEAAYLSAMIP
                     NPRTVFNPQVNPRRVARRQRIILRGMPYVKLP"
     gene            complement(1544..2497)
                     /locus_tag="VFO72_07380"
     CDS             complement(1544..2497)
                     /locus_tag="VFO72_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9479146.1"
                     /translation="MARANISSARMQVFYTPRYYADIGDGHIFPIRKFEFVRDRLLAE
                     GTLHPHELVEPSPAAIEHVRLVHTEDYVSRLCAGALTAKELRRLGLPWSESLVRRSFY
                     ASGGTIAAAHAALVDGYASNLAGGTHHAFADRGEGFCVFNDVAIAIRVLRKEGRIRRA
                     AIVDCDVHQGNGTATIFAENQDTFTFSVHGANNYPLFKARSNLDIELPDGTTDREYLD
                     CLTSQLPAVLATEPDIVFYLAGADPYAGDKLGRLALTIDGLRQRDVFVLRECYEREVP
                     VVTVMSGGYGKDINDTIEIHCNTIRMVKEVFEFQSAARRLA"
     gene            complement(2550..2960)
                     /locus_tag="VFO72_07385"
     CDS             complement(2550..2960)
                     /locus_tag="VFO72_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9479147.1"
                     /translation="MQARRLPGSDRTPDRMGQAREVAASGSPPERRRLLMGNPRPKPL
                     RLADKLLQIRKGLGLSQTQMLARMGLEETMHYGRISEYEQGKREPTLMTLLAYARAAS
                     VHLEDIVDDHLELPRKLPGTVHYRGLRRSSTRRK"
     gene            3397..3633
                     /locus_tag="VFO72_07390"
     CDS             3397..3633
                     /locus_tag="VFO72_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479148.1"
                     /translation="MRPTVSNHPFCNPFYNRFNDPVQRRYDDMQAYAAPSLNATPRTS
                     HSEPESNHSGQVFAGAFALIVVLVVSAVLLVEVL"
BASE COUNT          780 a         1037 c         1058 g          813 t
ORIGIN      
        1 gagacccggt tgcaagtctg aactcagttc ttccggcagt gaaagcgcag ccgaaccgtc
       61 ctgtgcctga gctcacaaac ctcaccctaa tggctgcgcg aatgaacgct ccggctgcac
      121 cggctatagc ttcggatact gtcgatccgg atctcgcgtg ggcagtcgac acttcgataa
      181 agagttggcg cgtgttcaag cctgccgggg aaaatttttc ggccgaaatg ccggcgggcg
      241 gtaaacaggt gacgatctct gttccgtttc aagatcaaat gttcgacttc aacgtctacg
      301 tggttcgtga cggcttcgcg atctactcgg cgatgtggat aaccgcgccg tcggatggcg
      361 aatcagacaa agcagcgctt atggtaacag tccgtgggtt gttgaaaggc atggttgaag
      421 gttacaagaa cgaaggaggc ggcccctttt cgtgtgagct ggaaagcgag agaaacgttt
      481 cgattaacgg atacacggga agtgaattcg atcttccggc gtgcacggtg cccggcaagg
      541 tgcgtgtctt tacgaaagtc gttggtgatc agaggcaaat gtactgcggc gcagtcttct
      601 actggcaaga cgacccgaac gtgtctcgtt tcctaaagtc ctttaccgcc ggtcccaccg
      661 cgcgagcgaa acgtcctgcg acaattagcg aacgttaaac ctgggaccgc gggcgtctcg
      721 cccgcaatcc agcgcatcgg acgtcgtcga ctctctgcgg gcgagacgcc gcggtcctta
      781 ctaattaagg cagctttaca tagggcatgc cgcgcaggat gatgcgctgg cggcgtgcga
      841 cgcgacgtgg gttgacctgc ggattgaaaa ctgttcgcgg gttgggaatc attgccgaca
      901 aataagcagc ttcgctggcg ttcagcgccg acgcaggctt gcgaaagtat ctctgggatg
      961 cggcttcggc gccgtaaatt ccatcacccc attcgatgac gtttagatag atttccagaa
     1021 tacgacgctt ggttagtaat cgctcgagca tgatcgtaac cagcgcttcc tgacctttac
     1081 ggacaaacga tcgctgcgac gacaggtaga gattcttcgc cagttgctgc gagatcgtgg
     1141 acccgccgcg tttgaactca ggcaggttcg gaagccagtc gtcattctcg ccttcgcgct
     1201 ttgcctcccg cgccgcttcc cgctgcgcct gctcgaatgc tttctgaatc gcctcgtagt
     1261 cgaatccgcg atgcgtgagg aagtttgtat cttcgccggc cagaaccgca cgctgtaagt
     1321 tgggggagat cctttgcaac ggcacccaga cctgttcgcg tcgcggttgc tgtcccctgg
     1381 ctcgcgcttc tctggcgcgc gcatcgatca gcgacgttga tgacggattg ctcgtgcgta
     1441 accaataaac gcgcagcatt agtatgcctt cgtaggcaat gacgcccacg atcacgccca
     1501 acactaccaa gaataaccag cgaaaaactc tgcgcatctg tattcaggcg agtcttcgag
     1561 ccgcgctttg aaattcaaat acctctttga ccatgcgaat cgtgttgcag tggatctcga
     1621 tggtgtcgtt gatgtctttg ccgtaaccgc ctgacatcac cgtgaccacc ggcacctcac
     1681 gttcgtaaca ctcgcgcaag acaaagacgt cacgttggcg caggccatct atcgtcaacg
     1741 ccaggcggcc gagcttgtcg ccggcgtacg ggtctgcgcc agccagatag aagacaatgt
     1801 cgggctcggt agccaaaact gccggcaatt gactagtcag gcagtcgaga tactcgcgat
     1861 ctgtcgtgcc gtcaggcagt tcaatgtcca aattagatcg cgccttaaag agcgggtagt
     1921 tatttgctcc gtgaactgag aaggtgaacg tgtcctggtt ttcggcaaaa atagttgcgg
     1981 tgccgttgcc ctggtgaacg tcacaatcga cgatcgctgc gcggcgaatc cgtccttctt
     2041 tacgcaagac gcgaatcgcg atggccacat cgttgaaaac gcaaaaccct tcgccacgat
     2101 cggcaaacgc atgatgcgta ccgcctgcga gattggaagc gtagccgtcg accaacgcag
     2161 cgtgtgcggc agcaatcgtg ccaccgctgg cataaaacga ccgccgcacg agcgactccg
     2221 accacggcag gcccaggcgt cgcagttctt tcgcagtcag agcgccggca cataggcgcg
     2281 agacgtaatc ctccgtatgt accaggcgca cgtgttcaat cgctgccggg gagggttcaa
     2341 ccagttcatg tgggtggaga gtgccttcgg ccaggagccg gtcgcgtacg aattcaaatt
     2401 tgcgaatggg gaagatgtgc ccgtcgccga tatcggcgta gtaccgaggt gtgtagaaaa
     2461 cttgcatgcg cgcggacgag atattagcgc gagccaagga tctacacaaa agagagacta
     2521 gtctgtgttc tcagtgttta tctgtggtct tacttgcggc gggttgagga cctgcgcagg
     2581 ccgcggtagt ggacggtgcc gggaagttta cgcggcagtt cgagatggtc gtcgacgata
     2641 tcttcgaggt gaacactcgc agcgcgcgcg taagcgagca gagtcatcaa ggtcggttct
     2701 cgtttgcctt gctcgtattc cgagatgcgg ccgtagtgca tggtctcttc cagacccatg
     2761 cgtgcgagca tctgtgtctg ggacaaaccc aaccctttgc gaatttgaag cagcttatcg
     2821 gcgagccgta atggcttggg tcgcgggttg cccatcagca gccgtctcct ttcgggagga
     2881 gaaccagaag ctgcgacttc gcgggcctgg cccatacggt ccggtgtcct gtctgagcct
     2941 ggtaaacgtc tcgcttgcat ctactcgcac ttgggagtaa tatgctgtcg ctgttgtttt
     3001 tcgccgtgag tatgaacctt tcatgctcaa cggcgatgct catcactcgt tagtgtggga
     3061 ggttgagccg cgcatatggc cgttgcgagt tgtcaaagac ggctgcatcg taaaacgaga
     3121 aggcccccgc cgtaaacaga aaaattacag tctcggttgg ccctgtgaag cgggccaatg
     3181 agtccgcgga tgccggaagg cccagagccg ctccgcggac tcccctttgc ccccattcac
     3241 cacttcgtgt atccgctcac ccaattgcgg ctcctgccgc actgccagtg ctgcaaaatg
     3301 cctcaaatga ctcgggtttt ttgcatcggc atggctgttg ctcttcgtgg tttcaattcg
     3361 gctacgcccc tcagccaaaa gaaaggagca gcggcaatga gaccaacagt ttccaatcat
     3421 cccttttgca atccgttcta caaccgcttc aacgatcccg tgcagcggcg ttacgatgac
     3481 atgcaggcct atgcggcgcc gtcgctgaac gcgacgccgc gaaccagtca ctcggaacca
     3541 gagtcgaacc acagcgggca agtattcgcc ggcgcatttg cgctgatcgt cgtcctggtc
     3601 gtctctgccg tactgctcgt cgaagtcctt tgagaaaccg cgctgattcc agtaatctgt
     3661 gccatccaca aattacaagg agcagagc
//
LOCUS       DASTEY010000415         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_684626, whole genome shotgun sequence.
ACCESSION   DASTEY010000415 DASTEY010000000
VERSION     DASTEY010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684626"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VFO72_07395"
     CDS             complement(<1..1829)
                     /locus_tag="VFO72_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9479149.1"
                     /translation="MKSKIGLLVLALSACVLLTATSAQLQNEQPKANAREEAYRANNL
                     GVALLEQFKHKEAADAFRRALQLQPGLALARINLGIALFNMPDLPAAQKELQAASVAS
                     PSAPQPLYVLGLAAKTQNKPEEAIVAFQKVLRIDPNDVGANVNLGQLYAQQRKYPEAI
                     AALRLAVAAEPYNATALYNLGTSLMRSGQREEGTKVIGQFQELRNRGTGTTLGTNYLE
                     QGRYAEAIASTGAEADLVDKQTPEVVFKDETPAALPPAGEWPADADAGAALLFDYDLD
                     GDLDLLEVAGNRQRLSRNDSGKFTDITAQAGALSKTATVSGTAAIAGDYDNDGRPDLF
                     ILRQGASTLYHNDDKGTFSDVTARAKIATPANLSSSAAFVDFDHDGDLDLLIAGDGYL
                     LLRNNGDGTFSDQTVAAKLADKVAVRAIVPTDFDNRRDIDLLVASTEKISLWRNLRDG
                     TFRDVATEVGITRKGATSVAAGDVNKDGFTDFYFAAANDTTGDFALSTGKERFQLTRT
                     EGGSNAAQFIDYDNDGLLDLVTSVQTQLRVLRNTGDAWADVSSKATQGLTATAGKRAP
                     VLLSGDLDGDGDTDIIFGTPGGGLKFARNNGGNANRSVRVNLAG"
     gene            1933..>2154
                     /locus_tag="VFO72_07400"
     CDS             1933..>2154
                     /locus_tag="VFO72_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479150.1"
                     /translation="MKKIALTILLAALCVLNACSRTSNLPTKSSPQYNELVRTFYIGL
                     AALQVGHDVQADAKLAQFTQLAASEPAGWA"
BASE COUNT          411 a          611 c          645 g          487 t
ORIGIN      
        1 cccgcgagat tcacgcgcac ggaacgattg gcgttgccgc cgttgttgcg ggcgaacttc
       61 aaaccgccgc cgggagttcc gaagatgatg tcagtatctc cgtcgccatc gagatctccg
      121 gaaagaagca ccggcgcccg cttgccggct gttgcggtta atccttgtgt agctttactg
      181 ctcacatcgg cccacgcgtc accggtattg cgcagcacgc ggagttgcgt ctgtacgctc
      241 gtgacgagat cgagcagtcc atcgttgtcg tagtcgatga actgcgccgc attgcttccg
      301 ccttcggtgc gcgtgagctg gaaacgctct ttgccggtgc tgagcgcgaa gtcgccggtc
      361 gtgtcgtttg ccgcagcaaa atagaaatca gtgaaaccgt ctttgttcac atcgccggca
      421 gcgacactcg tcgcgccttt gcgcgtgatt ccgacttcag ttgccacatc gcgaaacgtt
      481 ccgtcgcgca ggttgcgcca gagcgagatc ttttcagttg acgcaacgag cagatcgatg
      541 tcgcgccggt tatcgaagtc agtcggcacg atcgcacgca cagcgacttt gtcagcgagt
      601 tttgcagcga cggtctgatc gctaaacgtg ccgtcgccgt tgttccgcag cagcaggtat
      661 ccgtcgccgg cgattaagag gtcgaggtcg ccgtcgtgat cgaaatcgac aaaggccgct
      721 gacgacgaaa gatttgcagg cgttgcgatt tttgcccggg cagtaacgtc tgaaaaagtt
      781 cctttgtcgt cgttgtggta gagcgtgctc gcgccctggc gaagaatgaa gagatcggga
      841 cggccgtcgt tgtcgtagtc gccggcgata gctgcggtac ctgatacggt ggcggtcttc
      901 gagagtgctc ccgcttgtgc ggtgatgtcg gtgaactttc cgctgtcgtt ccgggagagg
      961 cgctggcggt ttcctgccac ttcgagcaga tcgagatcgc cgtcaagatc gtaatcaaag
     1021 agcaaggccg cgccggcgtc ggcgtcagcg ggccactcac ctgcgggcgg cagcgcagcc
     1081 ggagtttcat ctttgaagac gacttccgga gtttgtttgt caactaagtc agcttcagcg
     1141 ccggttgacg cgatcgcttc agcataacgt ccttgttcga gataatttgt accgagtgtc
     1201 gtgcccgtac cgcggttgcg caactcctgg aactgcccga tcaccttcgt gccttcttct
     1261 ctttgacccg aacgcatcaa cgacgttccg aggttgtaaa gcgccgtggc gttataaggt
     1321 tccgcggcga cggcgagccg gagcgccgca attgcttcgg gatacttgcg ctgttgcgcg
     1381 tagagctggc cgaggtttac gttcgcgccg acgtcgttcg gatcgatgcg cagcaccttt
     1441 tgaaacgcga caatcgcttc ttccggtttg ttctgcgtct tggccgcaag acctaaaacg
     1501 taaagcggct gtggcgctga tggcgacgcc acgctcgcag cttgcaactc tttctgcgcc
     1561 gcggggaggt cgggcatgtt gaaaagcgcg atgccgagat tgatgcgcgc cagcgccaga
     1621 cccggttgga gctgcagcgc acgccggaag gcatccgcgg cttctttgtg tttgaattgt
     1681 tcgagcagcg caacgccgag attattagcg cggtacgcct cttcgcgcgc gttcgctttc
     1741 ggttgttcgt tttgaagctg cgctgacgtg gcggttaaca ggacacaagc cgatagtgcc
     1801 agcaccagaa ggccgatttt agatttcatt gtgctacatt gtgcggagtt tcccgcaatg
     1861 gcgcaaaggc gcaaagatag aattcggaga aaatattata ggcctataag acttataggt
     1921 cctatatgac ctatgaaaaa aattgcgctc accattttgc tcgcggctct gtgcgtgctt
     1981 aacgcctgca gccggacgag caatctcccg accaaatcat caccgcaata caacgagctg
     2041 gttcgcacct tctacatcgg cctcgccgca cttcaggtcg ggcacgacgt gcaggccgat
     2101 gcgaagctcg cgcagtttac acaactcgcc gcaagcgaac ccgcgggctg ggcc
//
LOCUS       DASTEY010000416         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1014409, whole genome shotgun sequence.
ACCESSION   DASTEY010000416 DASTEY010000000
VERSION     DASTEY010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014409"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFO72_07405"
     CDS             complement(<1..310)
                     /locus_tag="VFO72_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479151.1"
                     /translation="MNSIRCRKMGAADVRDAFHMLSDFLREDEHYLASSQAYGDLGLK
                     GLNDALDLFLERPELGFVWMAYDDNGVAGVCVVCYAISTSMGSVVAKLDDVSVKSDRR
                     G"
     gene            418..>2457
                     /locus_tag="VFO72_07410"
     CDS             418..>2457
                     /locus_tag="VFO72_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479152.1"
                     /translation="MKKTITLFATALLLVAAISVFGREARLVRYPHYHNGRVVFTYLG
                     DIWTADENGQNVQRLTVNRARDVYGRFSPDGKWIAFSSDRTGNLDVFLIPAGGGQAKQ
                     LTHHSAADTVLGWSADSHSVLFSSSRGEDFAPQLYLVSTEGGMPWRAGTDMGVQASYS
                     PDGQKLAYNQKSQVYWRKYYRGAMQSDIMVMDVAAKKFTQLTDFDGLDSWPLWGRDGH
                     IYFVSDREGRGLTNIYRVSENGGKAERVTTFKSGDVRWPAISADGRVIMFEHDFGIWK
                     LDVNSKRATPITLNIAAETQENMSEVLSFNSQADDFDLAPSSRRVVLSIHGEIFTAPV
                     EEGDLKQLTDGPARDLNVAYSPDGKWISYVSDESGREELYVVPVDASAPAQKVTDIDV
                     LKLNYNWSPDSKEIAFSSSDNKLRKLTVASKQVVELDSSRYGALSAPVWSPDGKWIAY
                     AKPDISRTSDIYMIASSGAEQEPHRVTFDSNNEGSPRFAPDGRKLYFQRVEAAAGGNV
                     PTSVQIFSVWLERQEKDPDDPEERESEPPATPPAEGGEGAATPAARRAPPANRPPREI
                     KMDWDGLKRRTRQITRMPFPILNYTITPDSRTLVFVTTEPAAQAAIPVIYSIQQDGRR
                     LSRVTAGQPPTGGGGGPGGGGGGFGGGISNLQVSRDGRTLFFRERDGIWSVPLGGG"
BASE COUNT          541 a          741 c          686 g          489 t
ORIGIN      
        1 ttccacggcg gtcggatttg acggatacat catccagctt cgcgaccacg gatcccatcg
       61 aggtcgagat cgcgtaacac actacgcaga caccggccac accattgtcg tcgtaggcca
      121 tccagacgaa tccgagttcg ggccgttcga ggaagagatc gagcgcgtcg ttcagtcctt
      181 tcaaaccgag atcgccgtaa gcctgactgc tggccagata gtgttcgtcc tctctgagaa
      241 agtcgctcag catgtgaaac gcgtcgcgca cgtctgcggc gcccatcttc cggcagcgaa
      301 ttgagttcac gggccaatca tgccaattcg ccttcgcaat tcgcaagttt cagggatata
      361 atttgcgctc cgcattccag gtaattttcc aacccaatta agcgcaggtc aggagccatg
      421 aaaaagacaa tcactctctt cgcaacagcg ctgcttctcg ttgcagcaat ctcggtgttc
      481 ggccgcgaag cgcggctcgt tcgctatccg cattatcaca acggccgcgt cgtctttacg
      541 tatcttggcg acatctggac cgcagacgaa aacggtcaaa acgtgcagcg cctgacggtc
      601 aatcgcgcca gagacgttta tggacgtttc tctccggacg gcaaatggat cgcgttctcc
      661 agcgaccgaa ccggaaatct cgacgtgttc ctgattcctg ccggcggcgg ccaggcgaaa
      721 cagttaacgc accattccgc agcagacacc gtgctcggat ggtccgccga ttcgcattcg
      781 gttctgttct ccagcagtcg cggcgaggac ttcgcgccgc aactctatct cgtcagcact
      841 gaaggcggca tgccgtggag agccggcacc gacatgggcg tgcaggcttc gtactcgcct
      901 gacggacaga aactcgccta caaccagaag tcgcaggttt actggcgcaa gtattatcgc
      961 ggcgccatgc agagcgacat catggtgatg gacgtcgccg ccaaaaagtt tacgcaactg
     1021 acggacttcg acggtctcga ttcctggccc ctgtggggac gcgacggtca catctacttc
     1081 gtaagcgatc gcgaaggtcg cggtctcaca aacatctacc gcgtctcaga aaacggcggc
     1141 aaagctgagc gcgtcacgac gttcaagtcc ggtgacgtgc gttggccggc aatcagcgcc
     1201 gatggtcgcg tgatcatgtt tgaacacgac ttcggcatct ggaaactcga cgtaaattcc
     1261 aaacgcgcca cgccgatcac gttgaatata gcggcggaga cgcaggagaa catgagcgaa
     1321 gtcctgtcgt tcaactcgca agccgacgat ttcgacctcg caccatcttc gcgacgcgtc
     1381 gtgctttcga ttcacggcga aatctttacc gcgccggttg aggaaggcga tctgaaacaa
     1441 ctcaccgatg gaccagcgcg cgatctcaac gttgcttatt caccggacgg caaatggatc
     1501 tcctacgttt ccgacgaaag cgggcgcgag gaactctacg ttgtaccggt cgatgcttcg
     1561 gctccagctc agaaggtgac ggacatcgat gttctcaagc tgaactacaa ctggtcgccc
     1621 gattccaaag agatcgcttt ctcatcatct gacaataagt tgcgcaagct gaccgtcgcc
     1681 tccaaacagg tggttgagct cgacagttcg cgctacggcg cattgagcgc gcccgtctgg
     1741 tcgcctgatg gaaaatggat tgcttatgcg aagcctgaca tctcgcgtac gtcggacatc
     1801 tacatgatcg cgtcatccgg agcggagcag gaaccacacc gagttacgtt tgattccaat
     1861 aacgaaggca gcccgaggtt tgcaccagac ggccggaaac tctacttcca acgcgtcgaa
     1921 gccgcggcgg gtggaaacgt tccgacttcc gtccagatct tctcggtgtg gctcgagcga
     1981 caggaaaaag atcctgacga cccggaagag cgcgaatcgg aacctcccgc aacgccaccg
     2041 gcagaaggcg gcgaaggtgc agcaactccg gcagcacgcc gcgcaccacc agccaatcgt
     2101 ccgccgcgcg aaatcaaaat ggactgggac ggcttgaaac gccgtacgcg gcaaatcacc
     2161 cgcatgccgt ttcctattct caactacacc atcacacccg acagccgcac gctggtcttt
     2221 gtaacgactg aacccgcagc gcaggctgcc attccggtga tctactcgat tcagcaagat
     2281 ggccggcgtc tcagccgcgt taccgcggga caaccgccaa ctggcggtgg cggcggtccg
     2341 ggcggtggtg gcggtggttt tggtggcgga atttcaaatc tccaggtgtc gcgtgatggc
     2401 cgtacgctct tcttccgcga gcgcgatggc atttggtcag tgccgttagg cggtggc
//
LOCUS       DASTEY010000417         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1179047, whole genome shotgun sequence.
ACCESSION   DASTEY010000417 DASTEY010000000
VERSION     DASTEY010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179047"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VFO72_07415"
     CDS             complement(<1..1409)
                     /locus_tag="VFO72_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9479153.1"
                     /translation="MLYDMAAVNSNESAPVRPPAAGDMNSEEFRRFGHELIDWIAAYF
                     DRIEALPVLPPIQPGDLKTQLPAAPPQTGEPMEQIVADLDHLIVPALTHWNHPSFFAY
                     FATSTSGPGILGELLAAAFDNKAMLWRTSPASTELEEVTLDWLRQMMGLDPGMIGIIY
                     DTASVSSMHAIAAAREGVEQRIREDGMSGRPDLPLLRVYASEQAHSSIEKAVITLGLG
                     QRGLRKIPTDRDFRMDAKALAAAIEEDKRDGYLPFCIVATVGTTSTSSIDPVPEIIPI
                     GEQHAMWLHVDAAYAGSAAIVPEFKSILAGCERADSLVTNPHKWLFTPFDLSVLYCRH
                     LDLLKHAFSLVPEYLRTPEQEKVRNGSDYGIQLGRRFRALKLWMVIRYFGHEGLAARI
                     REHCRLADLFAAWVEESADWELLAPHPLALVCFRACPSSLPENELDALNEAIMHGVNS
                     SGRALLSHTRLNDKLTLRLS"
     gene            1667..>2083
                     /locus_tag="VFO72_07420"
     CDS             1667..>2083
                     /locus_tag="VFO72_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479154.1"
                     /translation="MANKDDVDLLPDFPYDVDVVERESWASQLGLVLAMAGNAVGLGN
                     FLRFPALAARNGGGTFMIPYFISLLLLGIPLMWIEWGIGRHGGKYKFSTSPGMFEVLW
                     KNPLAKYLGALGVFIPLAFVIYYTYIESWTLGYAVLS"
BASE COUNT          456 a          599 c          597 g          431 t
ORIGIN      
        1 gacaaacgca acgtcagctt gtcgttcagc cgcgtgtggg agagcaacgc gcggccgctg
       61 gaattaacgc cgtgcatgat cgcctcgttg agcgcgtcca gctcattttc cggcagcgaa
      121 gacggacacg ccctgaaaca aaccagcgcc agcgggtgtg gcgccagcaa ttcccaatca
      181 gccgactcct caacccacgc agcaaacaga tctgccaaac gacagtgttc gcgaatccgc
      241 gcagccaaac cctcgtgacc gaagtaacgg atcaccatcc ataacttcag cgctcgaaaa
      301 cgccggccca gttgaatgcc gtaatcggat ccgttgcgca ctttctcctg ttccggcgtt
      361 cgcaaatatt ccggcaccaa cgaaaacgcg tgcttcaaca gatccaggtg gcggcaataa
      421 agcaccgaaa gatcgaatgg cgtgaagagc catttgtgcg ggttggtcac cagcgagtcg
      481 gcgcgttcgc agccggcaag tatcgacttg aactccggca cgatcgccgc cgaaccggca
      541 tacgccgcat caacgtgcag ccacatcgcg tgttgctcgc caatcggaat gatctccggc
      601 actggatcga tactcgacgt agacgtcgtt cccaccgtcg ccacaatgca aaacggcaga
      661 tagccgtcgc gtttatcttc ttcgatagca gcggccaacg cctttgcgtc catgcgaaag
      721 tcacgatcag ttggaatctt gcgcaagcca cgctgtccca aacccagcgt gatcacggcc
      781 ttttcgatcg acgagtgagc ctgttccgaa gcgtagactc gcaacagcgg caggtcagga
      841 cggccagaca tgccatcttc gcgaatgcgc tgctcgactc cttcacgcgc cgcggcaatg
      901 gcgtgcatgc tcgagaccga agcagtgtcg tagatgatcc cgatcatgcc ggggtcgagt
      961 cccatcattt gccgcagcca gtcgagtgta acttcctcga gttccgttga cgcgggcgag
     1021 gtgcgccaca gcattgcttt gttgtcaaac gcggcggcca acagttcgcc gagaatgccg
     1081 ggaccggaag ttgaggtagc gaaataagcg aagaacgacg ggtggttcca atgcgtgagg
     1141 gccggcacga tgagatggtc cagatcagcg acaatctgtt ccatcggctc gccggtttgc
     1201 ggcggcgcag cagggagttg ggtctttagg tcgccaggct ggatcggcgg caagacaggt
     1261 aaagcttcaa tgcggtcgaa gtaggcggcg atccaatcga tgagttcgtg accaaaacgg
     1321 cggaactctt ccgagttcat gtctccggca gccggaggac gaacgggggc tgattcgttg
     1381 ctgttcacgg ccgccatatc atacagcata ggtcttcact gcccatggtt tacgagatgg
     1441 atgtcctgag ctgttggcgc gccgctaaag acagattttg tggacagatc agtcgaaatc
     1501 agtataatgc gcgagttatt cctgcattaa gctaatcgac tatcgagacc agcgccccaa
     1561 gtatcgcagc tttcgcgaat gccagagcag taaggcttgt cggtcgctgg tctattggta
     1621 aggccagagc gcagtaccca acgcaacttg cgaggagacc gactgtatgg cgaataagga
     1681 tgatgtcgat ctgttgcctg actttcccta cgacgttgac gtagttgagc gcgaaagctg
     1741 ggcctcacaa ctcggccttg ttcttgccat ggcagggaat gccgtcggcc tgggaaactt
     1801 ccttcgcttc cccgccctgg cagcacgaaa tggcggcggg acgttcatga tcccgtactt
     1861 catttctctc ctgctcctgg gcatcccgtt gatgtggatc gagtggggga tcgggcgaca
     1921 cggcggcaag tacaagttct cgacgtcgcc gggtatgttc gaagtgcttt ggaagaaccc
     1981 cctcgcgaaa tatctcggcg cgctgggagt tttcattccg ttagccttcg tcatttacta
     2041 cacctacatt gagtcgtgga ccctgggata tgccgtgctc tca
//
LOCUS       DASTEY010000418         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_932085, whole genome shotgun sequence.
ACCESSION   DASTEY010000418 DASTEY010000000
VERSION     DASTEY010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932085"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFO72_07425"
     CDS             <1..1130
                     /locus_tag="VFO72_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479155.1"
                     /translation="TARIQNNRTMMLASISTDIEARGRKGLPFLGGLPILGRLFTAPT
                     RDLRQVDIVISVTPRVLRAPAVTPRDEEMRPSGTLQSPTTGSLAEMIREADREDQIAA
                     ARAIPRNATIQLPDAPIEVAKPISAVPSEIQANNTTNNTATTNVPATTNSTTAQAAPA
                     PVQGKPVNSQSPGTVEELPSYVPAPKSLISDRAAAAVATVNPGTAANQNAVLTSLPKP
                     TEASLDTKLNTKLGAGRVAQLSLLQGDDVMKVGEKRRYAIQLNSDVSLSLALLTLKFD
                     PKVVKVHSVSAGSLLSTTGEGAPLFTPSIDASGVCMISISSLNGKASFRGSGALLFID
                     LEAVGAGNAALSFVKETLHLVATDARDVVSEIIQGTATVKQ"
     gene            1222..1845
                     /locus_tag="VFO72_07430"
     CDS             1222..1845
                     /locus_tag="VFO72_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9479156.1"
                     /translation="MKREQTEQGFSLMELVITVTVLTILTLGAMPLVKVSVKRQKEQQ
                     LRETLRQIRLAIDEFRRDTVGMVCTGTVVPPGGGGIAPPPGAIPVDPRSRVVISDCTI
                     FGIDNPDRYPPDLETLVNGVNVVPRFSGVGTDPNIPATELGQATSTKQKVYLRAIPID
                     PMTGKADWELRSSYDPADAGSWGGENVFDVRSKSKETALNGENYSDW"
     gene            1835..2257
                     /locus_tag="VFO72_07435"
     CDS             1835..2257
                     /locus_tag="VFO72_07435"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9479157.1"
                     /translation="MTGNLEQVRRRLHNVAHAGFTLLELMIVISIIIILAAITLPQYQ
                     KTIMHTREAVLRDDLFKMRSLIDQFAADKGRLPQSLEELVSEGYLREVPVDPFTGQKD
                     WAITTGEDVNSLTGEQGMTDVHSSSTEISTEGTPYSEW"
     gene            2410..>2731
                     /locus_tag="VFO72_07440"
     CDS             2410..>2731
                     /locus_tag="VFO72_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479158.1"
                     /translation="MPSLFSRIVDPWYPSTAIGLEKGLASVVQLERVKGSACRLKRAA
                     TANIGESLIRPSFDEPNIENSAQLAAVLNELAASAGLLRQKRWSLSLPEATARTIVFT
                     MESQS"
BASE COUNT          617 a          764 c          801 g          549 t
ORIGIN      
        1 gcacggcccg cattcagaat aaccgcacga tgatgctcgc cagcatctcg accgacatcg
       61 aagcgcgcgg ccgtaaggga ctcccgtttc tcggtggatt gccgattctg ggccggttgt
      121 ttacggcgcc aacgcgcgac ctgcgccagg tggacatcgt gatcagcgtg acgccgcgcg
      181 tgcttcgtgc gccggcagta acgccgcgtg acgaggagat gcgtccgagc ggtacgttgc
      241 aatcgccgac gaccggatcg cttgccgaaa tgattcgtga agcagaccgt gaagatcaga
      301 tcgccgccgc gcgtgctatc ccgcgcaacg caacgatcca gctgccggat gcgccgatcg
      361 aagttgcgaa accgatcagt gcggtaccgt cagaaattca ggcaaacaac acgactaaca
      421 acactgcgac aactaacgtc ccggcgacta cgaactcaac gactgcacaa gcagcgcctg
      481 cgccggtaca gggcaaaccc gtaaacagcc agtcgcccgg cacggtggaa gaattgccgt
      541 cctacgttcc ggcgccgaag tcgttgatta gtgatcgcgc ggcggcggct gtcgcgactg
      601 taaatccagg cactgcggca aatcagaatg ccgtgctcac ttcgttgcct aaacctacgg
      661 aggcttcgct cgataccaag ctcaacacca agttgggcgc gggccgtgtg gcgcagctga
      721 gtctgctcca gggcgacgac gtgatgaaag tcggcgagaa acgtcgctac gcaattcaat
      781 tgaactccga tgtttcgttg agcctggctt tgctgacctt gaagtttgat ccgaaggtcg
      841 tgaaagtgca cagtgtcagt gccggaagtc tgttgagtac gaccggcgag ggagcgccgc
      901 tattcacgcc gtcgatcgac gccagtggcg tctgcatgat ctcaatctcg tcgctgaacg
      961 gtaaggcctc gtttagagga tcgggggcat tgctgttcat cgatcttgaa gcggtcggcg
     1021 ccggcaacgc tgcgctgagt ttcgtgaagg agacgttgca tctggtggcc actgatgctc
     1081 gcgacgtggt ttcggagatt attcagggaa cggcaacagt caaacaatga cgtttctgcc
     1141 gcatggcaga gcgagacaat atgcggcgac tggatgagcg gagtttaatt cttaccggtc
     1201 gtcgcattct tctgcaggcc aatgaagaga gagcagacag agcagggatt ctcactgatg
     1261 gaactggtga tcaccgtgac ggtgttgacc attctgacgc tcggcgcgat gccgctcgtg
     1321 aaggtatccg taaagcggca gaaggaacaa caactgcgcg agaccttgcg gcagattcgc
     1381 ctggccatcg acgagtttcg tcgcgacacc gtagggatgg tttgtaccgg cacggtcgta
     1441 ccgcctggtg gcggcggcat tgcaccgcct cccggcgcta taccggtcga tccgcggagc
     1501 agagttgtca tcagcgactg cacgatcttt ggcattgata atcccgatcg ttatccgccc
     1561 gatctggaaa cgcttgttaa tggcgtgaac gttgtaccgc gattctccgg agtgggaacc
     1621 gaccctaata taccggcaac cgagttgggc caggcgacgt ccaccaagca gaaggtttac
     1681 ctgcgcgcga taccgatcga tccaatgacc gggaaggccg attgggaact ccggtccagt
     1741 tacgacccgg cagatgcggg ctcgtggggc ggtgagaacg tttttgacgt gcggtccaaa
     1801 tcgaaagaga ctgcattgaa tggagagaac tacagtgact ggtaatctcg aacaggtacg
     1861 acgcagatta cacaacgtag cacacgcagg attcacgctg ctcgagttga tgatcgtgat
     1921 ctcgatcatc atcatcctgg cggcgattac attgccgcag tatcagaaga ccatcatgca
     1981 cacgcgcgag gcggtgctgc gcgacgacct cttcaaaatg cgctcgctga tcgatcaatt
     2041 tgccgctgac aaaggacgct tgccgcagtc gctggaagaa ctggtcagtg aaggttacct
     2101 ccgcgaagtg ccggtcgatc cgttcacggg acaaaaagat tgggccatca ccacgggtga
     2161 agacgtcaac tcgctcaccg gcgaacaagg tatgaccgac gtgcatagca gttccactga
     2221 aatatccacc gaaggcacac cgtacagcga gtggtagcat tgccgatttt cgattgccga
     2281 ttggtggcat tgccgatttt cgattgccga ttgccgattt ttcggtcgcg gcagccggac
     2341 aaactgtgat gatctcaaat agcgcaaatc gaaaatcggc aatcgaaaat cgacaatcga
     2401 caataaccta tgcccagtct cttttcacgc atcgtcgatc cctggtatcc ctccacggcc
     2461 atcggtctgg aaaaagggct cgcttccgtt gttcagctcg aacgcgtaaa aggcagtgcc
     2521 tgccgcttga aacgcgctgc cactgcgaac atcggtgagt ctctgattcg gcccagcttt
     2581 gacgaaccca acatcgagaa tagcgcccag cttgccgccg ttctaaatga gctcgcggcg
     2641 agcgctggac tgctccgtca gaagcggtgg tcgttgagtt tgccggaagc caccgcgcgc
     2701 acgatcgttt tcacgatgga aagccagtcc a
//
LOCUS       DASTEY010000419         4144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_338756, whole genome shotgun sequence.
ACCESSION   DASTEY010000419 DASTEY010000000
VERSION     DASTEY010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338756"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VFO72_07445"
     CDS             <1..1103
                     /locus_tag="VFO72_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479159.1"
                     /translation="AWTPPYESTERVAWAGSYPNQPDLKFHVEAAAFAGKPVYFEIFH
                     AWDQPAAPVVSMARFKERALVVLLLSVFIIVMLASAALALRNLKLGRGDRKGAFRLAA
                     FLSAVLFLQWLFWSHHVATESEALNFIAGVQQILFWAFFFWVVYLAFEPFVRRRWPGR
                     IISWNRVLAGGFRDPLVGRDILVGALFGLGIVMCNFYLAQLVPQWLGRPPGIPWWDFP
                     ATQLLGIRSFAFGFPQQIFAAIFQSFVLLFFLLLLYIVLRGERVAAIALWLIAAAALS
                     LTQEAAAAVPFACLAAALAVWLLYRYGLLALIVAEFFLHLMIFYPVTSDFSAWYAGDF
                     VLALIISLALVGFGFYTSLAGEPLFRSTRLDD"
     gene            complement(1100..3676)
                     /locus_tag="VFO72_07450"
     CDS             complement(1100..3676)
                     /locus_tag="VFO72_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479160.1"
                     /translation="MPESVSQYSPQQLARYRALSIACALVTMSIGGAALLGWIFKIET
                     LKRIHPALVTMKANTAICLILVSVSLLVLRNPQAREWQRRVAQFFTVLVALVGLLTLT
                     EHLFGWDFRIDQALFQESAAAAGASFPGRMGVAASLNFLFLGFALLFLNARSSRWFRV
                     SNVAVLLVVAVTLLVFLYYFYGIERFDPIASYFTIALHTVVAFLSFCAAILFTRPDRG
                     LIAILLGNSQGGVVARRMWPALLLVVLLGWIRAIGRDAGLFGAGFGTALFVLSILVIF
                     VGLIWWTAVSLNRTDRQRRQAEKRLAVLVRVSELVRTLRDPYELSYAVAETVGVELDL
                     RRCLFNETDVEQDLEVVHRDYCDRTESVAGVHRISDYSSITSEAMKLGETVVNFDSQA
                     DPRTAADYRRSYEPTGERSYVAVPLLRDGRWVASLWASDDKPRQWTKDEISLLQTVGE
                     RTWTAIEKLRAEAERERLLQSEQEARDAAEKANQLKDEFLATLSHELRNPLNVILGYS
                     ELFLRLPEIGQSAQLRKMAESLRRNAQSQSQLINDLLDLSRLQRGKISLSQETVSLAA
                     IIDNAVETVRTEAAAKGIAINVNLDDQFLLVDGDRLRLQQIAWNILNNAVKFTPSGGS
                     INISLNREGKEAVLVVEDTGQGIDAGFLPHVFEMFRQADGSNVRRHGGLGIGLALVQQ
                     LVQLHGGTISAHSEGTNKGSRFTIRLPLTDEAAVHRMPAHTTLPLESSALDQTEFLIV
                     DDSEDTIAMLAQLLKLSGANVTAATNGVDALRLAGEREFDVILSDISMPEMDGFEFLQ
                     RLRQIDGRQHIPVIAITGFGRADDVERARAAGFHSHLTKPVDLDALAELLQQLTARVP
                     NV"
     gene            3876..>4144
                     /locus_tag="VFO72_07455"
     CDS             3876..>4144
                     /locus_tag="VFO72_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479161.1"
                     /translation="MAQPEQTRSAEARDVRIRSGAVQLYGDLTLPKGAPGVALFAHGS
                     GSSRHSPRNQFVAGVIREAGVGTLLFDLLTPEEEAIDARTGHLRF"
BASE COUNT          858 a         1164 c         1197 g          925 t
ORIGIN      
        1 cggcctggac tcccccgtac gaatcgactg aacgcgtcgc gtgggccggt tcatatccca
       61 accaacctga cttgaagttt cacgtcgaag ctgccgcgtt tgcaggtaaa ccagtctatt
      121 tcgagatctt tcacgcgtgg gatcaacccg ctgcgccggt ggtgagtatg gcgcgtttta
      181 aagagcgagc gctggtcgta ctactactgt cagtcttcat cattgtgatg cttgcgagcg
      241 cggcgctcgc tttacgcaac ctgaagcttg gccgcggcga tcgcaaaggc gcatttcgac
      301 tggcggcatt tctttcggca gttttgtttc tgcagtggct cttttggtcg catcacgttg
      361 caacagaatc tgaggcgttg aactttatcg ccggcgttca gcagatcttg ttttgggcgt
      421 tctttttctg ggtggtatat ctggctttcg agccgttcgt gcgacgccgc tggccgggcc
      481 gaatcatttc ctggaaccgg gtgctggctg gaggttttcg cgatccgctc gtcgggcgcg
      541 acatactggt cggagcactt tttgggctcg gcattgtcat gtgtaacttc tatttagccc
      601 aacttgtgcc gcaatggttg ggccgcccac cgggtattcc gtggtgggac tttccggcaa
      661 ctcaactctt aggaattcgt tcgtttgcat ttggcttccc ccaacagatc ttcgccgcga
      721 tttttcagtc gttcgtactg ctgttctttc tcttgttgct ttacatcgtt ctgcgcggag
      781 agcgggtagc ggcgattgcg ctctggttga ttgcggcggc agcgttgagt ttgacgcagg
      841 aagcggccgc ggcggtgccg ttcgcgtgtc tcgcggccgc attggctgtc tggcttttgt
      901 atcgttacgg gttgctggct ttgattgtcg ccgagttttt cctgcacctg atgatcttct
      961 atccggtcac ttccgatttt tcggcgtggt atgcaggtga cttcgtcctc gccttgatca
     1021 tctcgctggc gctcgtgggc ttcggtttct acacctccct cgctggcgaa ccattattcc
     1081 gctcaacgag gttggatgat taaacgttcg gcactctggc ggtgagttgt tgtagcagtt
     1141 cggcgagcgc gtcgaggtcc acgggtttag ttaagtgaga atggaaaccg gcagcacggg
     1201 cgcgttctac gtcatccgca cggccaaagc cggtgatcgc tatcaccggg atgtgttgcc
     1261 ggccgtcgat ctggcgcagc cgctgtagaa actcgaaacc atccatttcc ggcatcgaaa
     1321 tatccgagag aattacgtca aactcccgct caccggcaag ccgcaaagca tcgacgccgt
     1381 tagttgccgc cgtcacgttg gcgccgctga gtttgagtaa ctgagccagc atcgcgatcg
     1441 tgtcttcaga atcatcgacg atcaaaaact ccgtctggtc caaagcgctg gactcgagtg
     1501 gcaaggtcgt atgtgctggc atgcgatgca cggctgcttc atcggttaac ggcaaacgaa
     1561 tcgtgaagcg agatcctttg tttgtgcctt cagagtgagc cgagatcgtt ccgccgtgaa
     1621 gttgcacgag ttgttggacc agcgcgagcc cgataccgag gcctccatgc cgccggacgt
     1681 tgctgccgtc cgcctggcga aacatctcaa agacgtgagg caaaaatccg gcgtcgattc
     1741 cctgcccggt atcttcaacg accagcaccg cctccttccc ttcgcggttc aaactgatgt
     1801 tgatgctgcc gccactgggc gtaaacttca ccgcattgtt gaggatgttc caggcaatct
     1861 gctgcaagcg taaacggtcg ccgtcgacga gcaggaactg gtcgtcgagg ttcacattaa
     1921 tagcgatgcc cttggcggct gcttcagtac gcacggtttc aactgcgtta tcaataatgg
     1981 cggccagcga gaccgtctcc tgactcaaag agatctttcc gcgctgtaga cgcgacaaat
     2041 cgagcaggtc gtttatcaac tgtgattgcg actgagcgtt tcgccgcagt gattcggcca
     2101 ttttgcgcag ttgagccgat tgcccgatct ctggtaagcg caaaaagagt tcggagtaac
     2161 cgagaatgac gttcaaagga ttgcgcaact cgtgtgagag cgtcgccagg aactcgtctt
     2221 tgagttggtt tgccttctcc gctgcgtcgc gcgcttcctg ctcgctctgt agtaaccgct
     2281 cacgctccgc ttcggcgcgc agtttttcga tcgcggtcca ggtgcgttcg ccaacggttt
     2341 gcagcagcga tatctcgtct ttggtccatt gacgaggctt gtcgtcactg gcccacagcg
     2401 acgccaccca gcgaccatcg cgcagcagcg gaacggcgac atacgaacgt tcgcctgttg
     2461 gctcataact tctcctgtaa tcagcggccg tgcgtggatc ggcctgcgag tcgaagttga
     2521 cgaccgtctc gccaagcttc atcgcctcgg aggtgatgct gctgtagtcc gatatgcgat
     2581 ggacaccagc aacggactca gttcgatcgc aatagtcgcg gtggaccact tccaggtcct
     2641 gttctacgtc agtctcattg aacagacagc gacgaagatc gagctcgaca ccgactgttt
     2701 ccgcaactgc gtaagacagt tcataaggat cgcgcagcgt acgcaccaat tcgctgacgc
     2761 gaaccagcac cgccaaacgc ttctccgcct gacgtcgctg tcgatcggta cgattgagcg
     2821 acaccgcagt ccaccagatc agtccgacga agatcacgag tatgctgagt acaaacagag
     2881 cagtgccgaa ccctgcgcca aacaggccgg cgtcgcgacc gatagctcta atccagccta
     2941 agagaacaac cagcagtagc gcgggccaca tgcgacgcgc gaccactccg ccctgactgt
     3001 ttcccaaaag aatcgcgatg aggcctcgat ccggacgagt gaacaatatt gccgcgcaaa
     3061 aactgagaaa cgcgacgacc gtgtgcaacg cgatagtgaa gtaactcgcg atcggatcga
     3121 accgctcgat gccgtagaag tagtaaagaa aaacgagtaa agtgacggcg acgaccagca
     3181 gcacagcaac gtttgagacg cgaaaccaac ggctcgagcg cgcgttgaga aaaagcagcg
     3241 cgaagcccaa aaagaggaag ttgagcgagg cggcgacgcc catgcgtccc ggaaacgacg
     3301 caccggcggc agcagccgac tcctggaaga gcgcctgatc tatgcggaag tcccagccga
     3361 agaggtgttc ggtaagcgtc agcagaccga ctagagcaac cagcacggtg aagaactgag
     3421 ccacgcgtcg ttgccactcg cgcgcctgtg gatttcgaag cacaagcagc gataccgaaa
     3481 cgaggatgag acagatcgcc gtgtttgcct tcatcgtaac caaagcgggg tgaatacgct
     3541 tcagtgtttc gatcttgaag atccaaccca gcaacgccgc gccgccgatg gacatagtaa
     3601 ctagcgcgca cgcgattgag agcgctcgat agcgcgccag ttgttgcggc gaatactgcg
     3661 agacagattc cggcatgttg gcgatttttg actaagtttt tacgcgcgcg ctgtagtgcc
     3721 cactaccagt gcctcgtcgc cgctcccggc attcgagttg cgccggaaat tcgtagcact
     3781 ccgaaagata gcgagtgaat cgcagccctg cacgggaaag agacatctgc gcgtgcagag
     3841 tgtacgtgtg ggcaacaccg gcgaggttag caaagatggc gcaacctgag caaaccagaa
     3901 gtgcagaagc gagagatgta cgaattcgct ccggtgcggt gcaactttac ggcgatctga
     3961 cgttgccaaa aggcgcgccc ggtgtggcgt tgtttgcgca cggatccgga agcagtcgtc
     4021 acagtccgcg taaccagttt gtagccggcg tgattcgcga ggcaggtgtg ggaacattgt
     4081 tgttcgacct cctcacgccc gaggaggaag caatcgacgc gcgtacagga cacttgcgct
     4141 tcga
//
LOCUS       DASTEY010000420         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1459245, whole genome shotgun sequence.
ACCESSION   DASTEY010000420 DASTEY010000000
VERSION     DASTEY010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459245"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFO72_07460"
     CDS             complement(<1..246)
                     /locus_tag="VFO72_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479162.1"
                     /translation="MEKFLWIDPAGNSGELDCRLPIANCQLPIADLRPSALLKIGNRQ
                     SAIGNINMSEAEQFGFDIAVVGLAGRFPRARNLDEFWK"
     gene            complement(198..878)
                     /locus_tag="VFO72_07465"
     CDS             complement(198..878)
                     /locus_tag="VFO72_07465"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9479163.1"
                     /translation="MATPAFSRSTVDTLPTNKAREALVTCLKELKEDCGTLPRELAVV
                     ILGQWFHPLHYFPVFLSRLISVTPKVEAQTYISRILWEELGEGDPRRAHEKIYVETMV
                     HGGFEKAQFTEAPPLAATRKLLDGYEKASSDYLSGLGFLYGTEVVDLQMVATIGALMK
                     RCTGLRTLPWVDIHVAQEPGHVESSNQTLDQSLDQDEQHRVVTSAEEMWTLWKNFFGS
                     IRQEILAN"
     gene            complement(913..>2764)
                     /locus_tag="VFO72_07470"
     CDS             complement(913..>2764)
                     /locus_tag="VFO72_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HET9479164.1"
                     /translation="VDFPNRCIHELFEEQVERTPDAVAVAFGDEPLSYRDLNQRANQW
                     AHRLRRLGAKPETLIGICVERSVEMIVGLLAILKAGAAYVPLAPDSPPERLEWMLKGA
                     RIQLLLTQQKLLSKLPAEIAHLVALDGDQGVVESESQENPVTTTDPGNLAYVIYTSGS
                     TGRPKGVEVTHRNLVHSTSARFVYYDEPVRSFMLVSPFFFDSSVAGIFWTLCQGGKLV
                     LPPKDFERDITGFASLIEQEEVSHLLCLPMVHSLVLRQASPKRLKSLRCVIVAGEPCA
                     PELPKRHFATLSQASLFNEYGPTEGTVWTTVYNCAPQDHATTVAIGRPVPNVQTYVLD
                     DHRQLVPAGVIGELYIGGEGVARGYRNGPAATAERFVPHPFEAGARLYTTGDLARYLP
                     SGELEFLGRRDQQVKIRGYRIELGEIEIALVHHPAIREAAVVADDRRIIAYLTINGPA
                     SPTTEELRDFLRERLPEYMLPSQFVLLDAMPVGANGKVNRNALPAPGASRRAMTTSYA
                     APQTPLEQTIAEVWREFLQLDTIGVNDIFFELGGHSLLLVLVHERLQQRLGNDVPIAD
                     LFRFPTVRALARHLSGRPGAKAESSLNRQRAGERRAALKARSKTKAARNQ"
BASE COUNT          596 a          812 c          744 g          612 t
ORIGIN      
        1 cttccaaaat tcatcgaggt tccgggcgcg cgggaaacgt cccgcgagac caaccaccgc
       61 gatgtcaaac ccgaattgct ccgcttcact catattgata tttccaattg ccgattgccg
      121 attgccgatt ttcagaaggg ccgaaggtct taaatcggca attggcaatt ggcaattggc
      181 aatcggcaat cggcaatcta attcgccaga atttcctgcc ggatcgatcc aaagaaattt
      241 ttccaaagcg tccacatctc ttcggcgctg gtcactacac ggtgctgttc gtcctggtcc
      301 aaagactggt ccaatgtttg gtttgaactc tcaacgtgcc ctggctcctg cgcaacgtga
      361 atgtcgaccc acggcaacgt acgcaagccg gtacaccgtt tcatcagtgc gccgatggtg
      421 gccaccatct ggagatcgac gacttctgtc ccatagagaa aaccaaggcc cgacagatag
      481 tcgctcgacg ccttttcgta tccgtcgagt agtttgcgcg tcgcagcgag tggcggcgct
      541 tcggtaaact gcgctttttc gaatccgcca tggaccatgg tttcgacgta gatcttctcg
      601 tgcgcgcgcc ggggatcgcc ttcgcccagt tcttcccaga gaatccgaga aatataggtc
      661 tgcgcctcga cctttggcgt aaccgagatc aatctagaga ggaatacggg gaaatagtgc
      721 agcggatgaa accactggcc caaaataaca accgcgagct ccctgggcag agtaccgcag
      781 tcttccttca gctctttgag acaagttacc aacgcttctc gcgccttgtt tgtcggtaga
      841 gtgtctaccg tgcttctgga aaatgctggg gtcgccataa cttcgttacc ctttcttatt
      901 gatttttctg tgctactgat tccgcgccgc ctttgtcttt gatcgcgcct tgagtgccgc
      961 ccttcgctcg cctgcgcgtt ggcgattgag tgacgattct gcttttgcgc cgggcctgcc
     1021 gctgaggtgt cgcgccaacg cgcgcacagt cgggaacctg aaaagatcgg caatgggcac
     1081 gtcgttgccg agtcgctgct gcaatctctc gtgcacgaga acgagcagca acgaatgacc
     1141 gccgagttcg aagaaaatat cgttgacacc aattgtgtct aactgaagaa actctcgcca
     1201 gacctctgcg atcgtctgct cgagcggcgt ctgtggggcc gcataggaag ttgtcatcgc
     1261 ccgtcgcgac gcaccgggtg cgggcaaggc attccgattc actttgccgt ttgcacccac
     1321 gggcattgcg tcgagcaaca cgaattgcga cggcagcatg tattcaggca gtctctcgcg
     1381 caaaaagtcg cgcagctctt cagttgttgg agatgcaggc ccattgattg tgagataggc
     1441 aatgatacgg cggtcatcag caaccaccgc agcctcacga atcgcgggat gatggaccag
     1501 cgcaatttca atctcgccca actcgatacg ataaccgcga attttgactt gctgatcgcg
     1561 ccgtccgaga aactcaagct cgccgcttgg caggtaacgc gcgagatctc cagttgtata
     1621 caagcgcgcg cccgcttcga aaggatgtgg cacgaaacgc tcggccgtcg ccgccggacc
     1681 gttacggtat ccacgtgcca ctccttcacc accaatgtac agttcaccaa taacgccggc
     1741 cggcacgagc tgccgatgat cgtcgagtac gtacgtttgg acgttcggca ccggacgccc
     1801 gatcgccact gttgtcgcat gatcctgcgg cgcacagttg taaaccgtgg tccacaccgt
     1861 tccttcggtt ggtccatact cgttgaacaa cgaagcctgc gacagggttg cgaagtggcg
     1921 cttcggtaac tccggcgcac acggctcgcc ggcgacgatc acgcagcgga gactctttag
     1981 ccgcttggga gaggcctgac gcaataccag cgaatggacc atcggcaggc acaacaggtg
     2041 agaaacctct tcctgttcga tcaaactcgc gaagcccgtg atatctctct cgaaatcttt
     2101 cggcggcaac accagctttc cgccctggca caaggtccag aagattcctg ccacggaact
     2161 gtcgaagaaa aacggcgaga ccagcataaa gcttcgcacc ggctcgtcgt agtaaacgaa
     2221 acgtgcgctg gttgaatgga ccaggttgcg atgtgtaacc tcgacgcctt tgggcctacc
     2281 ggtcgatcct gaggtgtaaa tcacatatgc gagattgcct gggtccgtcg tcgtgaccgg
     2341 gttttcttgg ctttcgcttt cgacgacccc ttgatcgcca tccaatgcca ccaggtgagc
     2401 gatttcggcg ggcagcttac ttaacagctt ttgttgcgtc aggagcaact ggattctcgc
     2461 tcccttcaac atccactcga gacgctcagg cggagaatcg ggcgcaagcg gcacataagc
     2521 cgcgccggct ttcaggatag ccagcagccc gacgatcatc tccacggaac gttcgacgca
     2581 gataccgatc aaggtttcag gtttcgcgcc gagacgtcgc aggcgatgag cccactggtt
     2641 cgctcgctgg ttcaggtcac gatacgaaag tggctcatcg ccgaatgcga cagccactgc
     2701 gtcaggcgtg cgctccactt gttcttcaaa cagttcgtga atgcaacggt tcggaaaatc
     2761 gacg
//
LOCUS       DASTEY010000421         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1541827, whole genome shotgun sequence.
ACCESSION   DASTEY010000421 DASTEY010000000
VERSION     DASTEY010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541827"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1563)
                     /locus_tag="VFO72_07475"
     CDS             complement(58..1563)
                     /locus_tag="VFO72_07475"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET9479165.1"
                     /translation="MKRPPLLFLNVLIVATCGLIYELLAGTLASYVLGDSVTQFSLII
                     GIYLFAMGVGSWLSRFIEKGLARRFVDIELAVAILGGFSAPLLFLTFARVSYFYAVLY
                     FLVFAIGVFVGLEIPLLLRILKDQVEFKELVSRVLAFDYIGALLASILFPILFVPRLG
                     LVRTSLIFGMLNAAVALWGTWLLRPLIKGRITGLRVRSIVVLALLLVALVKANTLTSL
                     AEDEMFADEIVYTKDTPYQRIVITRGRAGFQLFLNGNLQFSSTDEYRYHEALVHPAML
                     VANNPRRVLVLGGGDGLALREILKYPSVEHVMLVDLDPEMTRLASRFPPLAELNKRAI
                     DDPRVRVVNEDAFIWIEQTSEAPYDAAVVDFPDPNSFSLGKLYTTRFYRLLRSRLTAN
                     AAVSVQSTSPMFARSSYWCIIRTLEASGFSVKPYYTAVPSFGLWGFALARSSPFEIPT
                     KPPEGLRFLDEPTLAAMFQLSKDIEPVPVEINRLDNQALVRYYEGEWKRFE"
     gene            complement(1648..1896)
                     /locus_tag="VFO72_07480"
     CDS             complement(1648..1896)
                     /locus_tag="VFO72_07480"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET9479166.1"
                     /translation="MLINLFVGLVVPLETLLPTLVTTLVFVLLGLIVFALAFLVIGKA
                     TPFSVRKEIEEDHNVALALVIASVILGSALIIAAAIHG"
     gene            complement(1896..2072)
                     /locus_tag="VFO72_07485"
     CDS             complement(1896..2072)
                     /locus_tag="VFO72_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479167.1"
                     /translation="MLKTLYTIFSIGVVSLYSAAAMLGWEIGGGTRRQLPPDARQNGY
                     RSFHFWHYGLRGGK"
     gene            complement(2198..4162)
                     /locus_tag="VFO72_07490"
     CDS             complement(2198..4162)
                     /locus_tag="VFO72_07490"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HET9479168.1"
                     /translation="MSVLVANCPACGGPVEFKSGQSIVVICNYCRSAVARTDRGLKDL
                     GKVAELVQTGSPLDVGLRGKWKDQHFELTGRAQLGHEMGGQWDEWYATFSNGWLGWLA
                     EAQGRFYLTFQYQIPEGVQVPPFDQLQLGQAINGLPWPTPLIVAETGRATALGAKGEI
                     PYLLTPGDTYFYADLSGTNRSFGTLDYNESPPLVFLGQEVTLAELGITTTRAPEREER
                     RVGAAHVSCPNCAGPLELRAPDKTERVTCPNCNSLLDVNQGQLQFLKTLEPPSFQPLI
                     PIGSTGEVPEGKMTVIGAMQRSVTIEGEQYFWNEYLLYNPQIGFRWLVHSDDHWNYVR
                     AVPPGEVTESAKFAHYRGTNYKIFQDAQARVESVLGEFYWKVEAGEQVRGMDYVAAPY
                     MLSKEVSTVYISDPKDASKQRRATGEINWSLGTYVTVPQIEKAFSVKDLPRPSNIAPN
                     QPYPHKWVYKYWIAFIALLLLVGMVTFLISGSSKEVFSETVNFPPLASPDATQVYFSQ
                     PFEMDGRSNIRVVGQAPVQNAWVYLEGDLINEESGVVQSFPINISFYQGVEDGEAWSE
                     GGQTDSAYTSALPAGRYVLRLEGQWEHWQQPARVSVKIEQNVARGFNLLVALIVLSIA
                     PIAMLIYHISFEHRRWSESMFHGGGSSDDD"
     gene            complement(4159..4545)
                     /locus_tag="VFO72_07495"
     CDS             complement(4159..4545)
                     /locus_tag="VFO72_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HET9479169.1"
                     /translation="MTLGTEWLIDASGCDPEALADLESLRAVFNRVINDLSLNVVGEI
                     AWAKFPHPGGVSGLALLSESHLACHTYPEFHAATFNLYCCRNRADWPWEERLKEMLSA
                     TEVRVRLFERMLHQSTPELEQAGAAR"
BASE COUNT         1087 a         1307 c         1318 g          958 t
ORIGIN      
        1 caacggccgc tggaagacca aggaaagcgg caaggatttc gcgacgatta aacgttgtca
       61 ctcaaatcgc ttccattcac cttcgtaata acgaaccagg gcctgattat cgagccggtt
      121 gatctccacc ggcaccggct caatgtcttt cgacaactga aacatcgcgg ccagcgtcgg
      181 ctcgtccagg aatcgcaaac cctcaggagg ttttgttgga atctcgaacg gagaggagcg
      241 ggcgagtgca aatccccaca atccaaaact cggcacggct gtgtagtaag gtttgactga
      301 aaaacccgag gcttcgagcg tgcgaataat gcaccagtaa gaactgcgcg cgaacatcgg
      361 cgacgttgat tgcacgctga ccgccgcgtt cgcggtcaat cgactgcgca agagccggta
      421 gaaacgcgtg gtgtagagct tgcccagcga gaacgagttt ggatcgggaa agtcgactac
      481 cgcggcatcg taaggcgctt ctgaggtctg ttcgatccag ataaacgcgt cttcattgac
      541 aacccgcacg cgcgggtcgt cgatggcgcg cttgttcaac tccgccagcg gcggaaaacg
      601 actcgcaagc ctcgtcattt ccggatcgag atccaccagc atcacatgct cgaccgaggg
      661 atatttcaaa atctcccgca acgcgagtcc atctccaccg ccaagcacca gcacacgccg
      721 tggattattc gcgacgagca tcgccggatg gaccaacgct tcgtgatagc gatattcgtc
      781 agtcgagctg aactgcaagt ttccattcag aaacaactga aaaccggcgc ggccgcgcgt
      841 gatgacaatg cgttggtagg gcgtgtcttt cgtgtagacg atctcgtcgg caaacatctc
      901 gtcttccgcg agcgacgtaa gcgtgtttgc tttgacgagc gccaccagca acaacgcgag
      961 caccacgatc gaacgcactc gcagaccggt gatgcgtccc ttgattagcg gacgcagcaa
     1021 ccatgtgccc cacaatgcaa cggccgcatt caacattcca aagatcagcg acgtgcgcac
     1081 cagacccagg cgcggcacaa acagaatcgg aaacagaatc gacgcgagca gcgcgccgat
     1141 gtagtcaaac gcgagcaccc gcgacacgag ctctttgaac tcgacctggt ccttcaagat
     1201 gcgcaacagc aacgggatct cgagtccgac aaacacgccg atagcgaaca cgagaaagta
     1261 gagcacggcg tagaagtagc tgacgcgcgc aaaggtgaga aacagtagag gcgcggaaaa
     1321 gccgccgagg attgcgaccg ccaactcgat atcgacgaag cgtcgcgcga gtcctttttc
     1381 gatgaaacgt gagagccatg agccgacgcc catggcgaag agatagatgc cgatgatcag
     1441 tgaaaattga gtgacggagt cgccgagaac gtaactcgca agtgtgcccg ccaggagttc
     1501 ataaatcaga ccgcaggtcg cgacaatgag aacgttcaga aagagtagcg gcgggcgttt
     1561 catgtcccta cggaaaaact ccgtgtctgt gtgtctccgt ggtgttgtgc tgggcattag
     1621 ccgccgagac acagagccta acgcgtgtca gccgtggatc gccgcggcga tgatcagcgc
     1681 gctgccaaga atcactgacg cgatcacgag cgcaagcgcg acgttgtggt cctcttcgat
     1741 ttccttgcgg actgaaaaag gcgtcgcctt tccgatgacg agaaacgcga gcgcgaagac
     1801 aatcagtccc agcaggacga acaccaacgt cgtgacgaga gttggcagca gtgtttcgag
     1861 tggtactacc aagccgacga ataaattgat cagcatcatt ttcctccgcg caaaccgtag
     1921 tgccagaagt ggaacgagcg ataaccattc tgccgcgcgt caggcggcaa ctgccggcgc
     1981 gtaccgccgc cgatctccca accgagcatc gccgccgcgc tgtacaagct caccacgccg
     2041 atactgaaaa ttgtgtagag tgttttgagc atttggaacg tcctctttag gttttagtct
     2101 cgcgcaaagg cgaaaggaag cacttgaaac aattcgcgat gccttttctt cgcgcttttc
     2161 gcgcgttgcg actttccttt gcgcctttgc gcgagaccta atcgtcatca ctggagccgc
     2221 caccatgaaa catgctttcg gaccagcgtc gatgttcaaa acttatgtga tagatcaaca
     2281 ttgcgatcgg agcgatggat aacacaatca gcgccaccag caggttgaac ccacgcgcta
     2341 cgttctgttc gatctttacc gaaactctcg ccggttgttg ccagtgttcc cattggcctt
     2401 cgagccgcag cacataccgt cccgcgggca gcgcggaagt gtaagccgaa tctgtttgcc
     2461 cgccttcgct ccaggcttcg ccatcttcga ctccctggta gaaggagatg ttgatcggaa
     2521 acgattgcac cacgccggat tcttcattga tcaaatcacc ttcgagatat acccacgcgt
     2581 tctgtaccgg cgcttggcca acgaccctga tgttgcttcg accgtccatt tcaaacggtt
     2641 gactgaagta cacctgagtg gcatcgggac tggcgagcgg tggaaaatta actgtttccg
     2701 agaacacttc cttgctcgaa cccgaaatca aaaatgtgac cattcccacc aacaacaaca
     2761 gcgcgatgaa tgcgatccaa tatttgtaaa cccacttgtg cggataaggt tgattcggcg
     2821 cgatgtttga cgggcgtgga agatctttga ccgagaacgc cttttcgatc tgcggaaccg
     2881 tgacgtacgt tccgagcgac cagttgatct cgccggtggc gcgccgctgt ttcgaagcat
     2941 ccttcggatc ggagatgtag acggtcgaga cttccttcga caacatgtaa ggcgcggcga
     3001 cgtaatccat gccgcgtacc tgctcgcctg cttcgacttt ccagtaaaac tcaccaagaa
     3061 cggactcaac ccgcgcctgc gcgtcctgaa agatcttgta gttcgtcccg cggtagtgcg
     3121 cgaacttcgc cgactccgtc acttcgccgg gcggaacggc ccgcacgtag ttccagtgat
     3181 cgtcgctgtg aaccagccaa cggaaaccga tctgagggtt gtagagaaga tactcgttcc
     3241 aaaaatactg ctcgccttcg atggtcacgc tgcgctgcat cgcgccgatg accgtcattt
     3301 ttccttccgg cacttcgccg gtcgaaccga tcggaatcag tggttgaaat gacggcggct
     3361 cgagtgtttt tagaaattgg agttgtccct ggttgacgtc gagtaacgaa ttgcagtttg
     3421 gacaggtgac tcgctcggtc ttgtcaggcg cgcgaagttc aagggggccg gcgcagtttg
     3481 gacaacttac gtgggcggcg ccgacgcgac gctcttcacg ttcgggagcg cgcgtggtgg
     3541 tgatgccgag ttcggcaagt gtgacttcct gaccgaggaa gacgagcggc ggtgattcgt
     3601 tgtaatcgag cgtgccgaac gaacgatttg tgccggacag atcggcgtag aagtaagtat
     3661 cgcccggcgt gagcaggtat ggaatctcac ccttagcgcc caatgccgtc gcacgacctg
     3721 tttctgcgac tatcagcggc gtgggccacg gcaagccgtt gatcgcttgt ccgagttgta
     3781 gttgatcgaa tggaggaacc tgaacccctt ccgggatctg atattgaaac gtcagataaa
     3841 aacggccctg cgcttcagcc aaccaaccaa gccaaccgtt ggaaaacgtc gcgtaccatt
     3901 catcccactg gccgcccatt tcgtggccaa gttgagcgcg gccggtcagt tcgaagtgtt
     3961 gatccttcca cttgccgcgc aggccgacgt cgagcggcga tccagtctgg accagctccg
     4021 cgactttgcc gaggtctttt agtccgcggt cggtgcgggc gactgccgaa cggcaatagt
     4081 tgcaaatgac gacgattgat tgtccgctct tgaattcgac cggtccgcca caggccgggc
     4141 aattcgcgac gagcacactc atctggcggc tccggcttgc tcgagttcgg gtgtggattg
     4201 atggagcatt cgttcgaaga ggcgcacacg gacttcagtc gcgctgagca tctctttcag
     4261 tctctcttcc cacggccagt ctgcgcgatt gcggcaacag tagagattaa aagtggcggc
     4321 gtgaaattcc ggatacgtgt gacaggcgag gtgtgactcg gaaagcagtg cgaggccgct
     4381 gacgccgcct ggatggggaa acttggccca ggcgatctcg ccgacaacgt tgagactaag
     4441 atcgtttatg acgcgattga aaactgcgcg caggctttcg agatcggcaa gcgcctcggg
     4501 gtcgcagccg cttgcgtcga tgagccattc agtgccgaga gtcatcttaa tcggtgaaca
     4561 aacgcggaca gtttacccga aaagtttgat tgggtaacag cggatttgag gttgcactga
     4621 tagccatgtc tggagaacag tgagcaaact caacaaatca gcgtacggtt
//
LOCUS       DASTEY010000422         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_635833, whole genome shotgun sequence.
ACCESSION   DASTEY010000422 DASTEY010000000
VERSION     DASTEY010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635833"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..829)
                     /gene="trpA"
                     /locus_tag="VFO72_07500"
     CDS             complement(47..829)
                     /gene="trpA"
                     /locus_tag="VFO72_07500"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET9479170.1"
                     /translation="MGRIADVFNSLKQQGQKGFIPYITAGDPDLATTEELLVALAQNA
                     TLIELGVPFSDPMADGPVIQRASERALKHNFGLQELLDTAAGARKRTDVPIILFSYYN
                     PLLQFGLKRVAQAVKDAGLDGVLVTDLTPEESGEFEAELHAQGLDMIFLIAPTSTDER
                     LKLVAERASGFIYAVSRAGVTGTRNTVSAEAETLVNRMRQFSSLPIAVGFGISNAEQV
                     ADVHRYADAVVVGSAIVAEMERLAGSPELVERVGKFSTGFRS"
     gene            complement(834..>2010)
                     /gene="trpB"
                     /locus_tag="VFO72_07505"
     CDS             complement(834..>2010)
                     /gene="trpB"
                     /locus_tag="VFO72_07505"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9479171.1"
                     /translation="LGHWGKYGGRFVPETLMAPLEELTAAYLATRDDQSFQSELQDLL
                     QNYAGRPTPLFYAARMTAHCGGASIYLKREDLLHTGAHKINNTLGQVLLARRMNKQRI
                     VAETGAGQHGVATATVCALFALNCVVYMGAEDMRRQALNVFRMQLLGAEVREVNAGSR
                     TLKDAINEALRDWVTNVADTYYLLGSALGPHPYPLMVRDFQSVIGREARAQILEREGR
                     LPDALIACVGGGSNSIGLFHPFLSDSEIRMIGVEAGGSGNELGQHAARFNAAGGGRPG
                     VLQGTMSYVLQDGNGQVAATHSISAGLDYPSIGPEHAFLHDAGRVEYVSVSDSEALEA
                     FQLLSRLEGIIPALESAHAVAQAIKVARDMRRDQILIVNLSGRGDKDVNSVREALEL"
BASE COUNT          436 a          597 c          575 g          402 t
ORIGIN      
        1 agcggcgtca agccgccgca ctccaaagag gcgcccatta aactgctcag gacctaaagc
       61 ccgtcgaaaa tttaccgacg cgttccacga gctcgggcga gcctgcaagc cgttccatct
      121 ccgcaacgat cgccgaaccc actaccactg catcagcata acgatgcacg tcggcgactt
      181 gctctgcgtt tgagatacca aaaccaaccg cgatcggaag cgaggaaaac tgtcgcatgc
      241 ggttcacaag tgtttccgct tcggcgctca cggtgttgcg agtgccggta acgcctgcac
      301 gagaaacagc gtagataaat ccgctcgcac gctcggcaac cagcttcaga cgttcgtcag
      361 ttgaagtcgg cgcaatcaaa aagatcatgt cgagaccttg agcatgcaac tctgcttcaa
      421 actcgccgga ctcttccggc gtgagatcgg taaccagaac accgtcgagc ccggcgtctt
      481 tgactgcttg cgcgacgcgc ttcaaaccaa actggagcaa gggattgtag tagctgaaaa
      541 gaatgatcgg aacgtccgtg cgttttcgcg cgccggcagc agtgtctaga agctcctgca
      601 aaccaaagtt gtgcttgaga gcacgttcgg aggcgcgttg aattaccggc ccatctgcca
      661 tcggatcgct aaacggcacg ccgagctcaa tcaacgtcgc gttctgagcg agtgcaacca
      721 ggagttcttc ggtagtagcg aggtcaggat cgcccgcggt gatgtacgga atgaagccct
      781 tctgtccctg ctgcttcaag ctgttgaaaa cgtctgcgat acgtcccacg ctgttatagc
      841 tccaacgctt cgcgcacgga gttaacgtct ttatctccgc gtcccgaaag attgacgatc
      901 aggatctgat cgcgtcgcat atcgcgcgcg acttttatcg cttgtgccac ggcgtgcgcc
      961 gattcgagtg ccggaatgat tccttccaga cgagaaagaa gttgaaacgc ttccagcgcc
     1021 tcgctgtcgg aaacggagac gtactcaaca cggcctgcat cgtgcagaaa cgcgtgttct
     1081 ggtccgatcg agggatagtc gagaccagcg gagatcgagt gagtcgcggc gacctggccg
     1141 ttgccgtcct gcaagacgta gctcatcgtt ccctgcaaca cgcccggacg cccgccgcca
     1201 gcagcgttaa agcgagcggc gtgctggccc aactcgttgc cggagccacc tgcttcgacg
     1261 ccgatcatac ggatctctga atccgataag aacggatgga atagaccgat cgaattcgag
     1321 ccgccaccga cgcacgcgat cagggcatca ggcaagcggc cttctcgctc gaggatctgt
     1381 gcgcgcgcct cgcgtccgat gacgctctga aagtctcgca ccattaacgg ataaggatgt
     1441 gggccaagcg cgctgccgag caggtagtaa gtgtcagcga cgtttgtgac ccaatcgcgc
     1501 agcgcttcgt tgatcgcgtc ctttaaagta cgcgagccgg cgttaacttc gcgcacttcc
     1561 gcgccgagca gttgcatgcg aaaaacattc aacgcctgcc ggcgcatgtc ttcagcgccc
     1621 atgtagacca cgcaattcag tgcgaacagc gcgcagacag tagcggttgc cacgccgtgt
     1681 tgtcccgcgc cggtttccgc gacgatgcgt tgcttgttca tgcgtcgggc gagcagcacc
     1741 tggccgagtg tgttgttgat cttgtgcgcg cctgtgtgca ggaggtcttc gcgtttgaga
     1801 tagatggagg ctccaccgca gtgggcagtc atgcgggcgg cgtaaaagag cggcgtgggc
     1861 cggcctgcgt aattctgcaa tagatcctgg agctcagatt ggaacgattg atcgtccctg
     1921 gtggcgagat aagccgcagt caactcctcg agcggagcca tcaaggtttc cggcacaaac
     1981 cttccgccgt actttcccca atgaccaagc
//
LOCUS       DASTEY010000423         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_602201, whole genome shotgun sequence.
ACCESSION   DASTEY010000423 DASTEY010000000
VERSION     DASTEY010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602201"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1191)
                     /locus_tag="VFO72_07510"
     CDS             complement(22..1191)
                     /locus_tag="VFO72_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET9479172.1"
                     /translation="MTFPNQPHLPAQAPTIIIARSPQVSGIKFAVGILAILFALCLGL
                     LVLIVIGIETGPVALMLGLITATIPVPLYIILVLWIDRYEAEPLWMLATAFFWGALVA
                     AFFAFLLNTTSRDVVRALANANAGEAFAAVISAPIVEELGKAFILLIFFFWKKDEFDG
                     VVDGIVYASMTALGFAMTENILYYGKAAAEGGGEALTLTLIIRGFFAPFSHPLFTSLT
                     GIGLGLARQSTNLLVKVVTPVVGLLMAIFMHSIWNGSAVFGGGAVFLLTYVLVMVPAF
                     VILLVVISLALRREGQVVREFLVIDLERGTLTREEYDQLGSIVGRMGSSFRAFSESGV
                     KGWRARRRFNQLASELAFHRSRVSRGVHPASNDTHGQEAAYLQALQQLVNELSPR"
     gene            complement(1215..1991)
                     /locus_tag="VFO72_07515"
     CDS             complement(1215..1991)
                     /locus_tag="VFO72_07515"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9479173.1"
                     /translation="MSDKRFAGKVALVTGATSGIGQACAIAFATEGAKVACVGRKESA
                     LQEMAERIRRFDVEALTIRADLSLPAEPERTVNETIETLGGIDVLVNAAGHISSGTIE
                     NTSLEAWDDMMNVNVRAAFQLMQKASPSLIERRGNIVNVSSVTGLRAFPGVLAYCVSK
                     AALDQLTRCASLELAAKGVRVNAVNPGVVVTEIHKRGGMSENAYTAFLEHSKTTHPLG
                     RTGRPEEIAALVLFLASDEASWITGATYSIDGGRALTCAR"
BASE COUNT          522 a          655 c          558 g          446 t
ORIGIN      
        1 gagacgcctg cggtcccagg attaacgcgg gctgagttcg ttaaccagtt gctgcaacgc
       61 ctgcaagtag gctgcttcct gcccatgcgt gtcgttgctg gctggatgaa ctccgcggga
      121 gacacggctg cgatgaaacg cgagttcgct cgcgagttga ttgaaacgcc tgcgcgctcg
      181 ccagcccttc acgccggatt ctgaaaacgc cctgaacgac gatcccatcc gtccaacgat
      241 cgacccgagc tgatcatatt cttctcgcgt caaggtgccg cgctcgagat cgatgacgag
      301 aaactcccgc accacctgcc cctcgcgccg caaagctagt gagatcacaa ctagcaggat
      361 tacaaatgcc ggcaccatca ctaatacgta agtcaataaa aacacggcgc cgccaccaaa
      421 cacggctgag ccgttccaga tactgtgcat gaagatcgcc atcaacaagc cgacaaccgg
      481 cgtcacaacc ttcaccaata agttcgttga ctgtctcgcc aaacccagac cgattccggt
      541 aagactggtg aacagcggat gcgaaaatgg cgcgaagaac ccacgaatga tcaacgtcag
      601 tgtcaacgct tcaccaccac cttcagccgc agccttgccg tagtagagaa tgttctccgt
      661 catcgcaaac ccaagcgctg tcattgaagc gtagacgatg ccgtcgacca cgccgtcgaa
      721 ctcatccttc ttccagaaaa agaagatcaa cagaataaaa gccttgccga gttcttcaac
      781 gatcggagct gagatcacgg cagcgaacgc ttcgcccgca ttcgcgtttg cgagcgcacg
      841 cacaacatcc cggctagttg tgtttagtag aaaggcgaag aaggcagcga cgagcgcgcc
      901 ccagaagaac gccgttgcca gcatccataa aggctctgct tcataccgat cgatccataa
      961 cacgaggatg atatagagcg gcaccggaat cgtcgccgtg atcagcccaa gcatcaaggc
     1021 gactggacca gtttcgatac cgatcacaat caaaacgagt agaccaagac acagcgcgaa
     1081 aagtatcgca aggatcccaa ccgcaaattt gatcccgctc acctgaggcg aacgcgcgat
     1141 gatgatcgta ggcgcttgag ccggtaaatg tggttgattt ggaaacgtca tgagtcgcgc
     1201 cagtatagtc gggactatcg agcacaggtt agcgcccgcc caccatcgat ggaataagtt
     1261 gcgcccgtaa tccaggaagc ctcgtcggac gcgaggaaca ggaccagcgc cgcaatctct
     1321 tccggccgtc cggtgcggcc aagcggatgc gtagttttgg agtgttcaag aaaagctgta
     1381 tacgcgtttt cgctcatgcc gccacgttta tgaatctcgg taaccaccac cccgggattc
     1441 acggcattca cgcgcacgcc cttagccgcc aattccagcg aagcgcaacg cgtcaattgg
     1501 tccaacgccg ctttggaaac acagtaggcc agcactcctg gaaacgcgcg cagtcccgtc
     1561 acactcgaaa cgttaacaat gtttccacgt cgttcgatca ggctcggcga cgccttctgc
     1621 atcagttgaa atgcggcacg tacgttaacg ttcatcatgt cgtcccatgc ttcgagcgag
     1681 gtgttctcga tagtgccgct gctgatgtgt ccagccgcat taacgagcac gtcgatgccg
     1741 cctaaggtct ctatcgtctc attgaccgtg cgttctggct cggcgggtag cgagaggtct
     1801 gccctaatcg tcaacgcttc aacatcgaat cggcgaatac gttcggccat ctcttgcaag
     1861 gccgattctt ttcggccgac gcacgccacc tttgctcctt cagtggcgaa tgcgatggca
     1921 caggcttgtc cgattccgga agtcgcgcct gtgaccagcg cgacctttcc tgcaaatctt
     1981 ttgtcgctca tgtttcggtc ctcgtctgct ttgaatggaa agagatcgta tgttgacagc
     2041 acaatcccaa aaagccgact attatcacct tgagaggctt gtgaggccat ccccccggca
     2101 caatgagtag tgatgcagtt tcagccagaa tgactgtttt acctgggcgt gctcttccct
     2161 gggaccgcag gcgtctcgcc t
//
LOCUS       DASTEY010000424         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1278408, whole genome shotgun sequence.
ACCESSION   DASTEY010000424 DASTEY010000000
VERSION     DASTEY010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278408"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFO72_07520"
     CDS             complement(<1..441)
                     /locus_tag="VFO72_07520"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET9479174.1"
                     /translation="MWHTCAPVTIAHLWSGAPAPVERHAEARICWSDEALHVRFVGNQ
                     HEPLVVSAEPKTDRKTLGLWDRDVCEIFVAPNPENLSRYFEFEAAPTGEWVDLGIVLA
                     AEGRQTDWDFSSGLTVASKLAPSQIKIALNIPWSEWIPKPDRNDR"
     gene            complement(477..1022)
                     /locus_tag="VFO72_07525"
     CDS             complement(477..1022)
                     /locus_tag="VFO72_07525"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479175.1"
                     /translation="MRIKIGVMGSAAPDSTRLETGKVLVEKAELLGREIASRNVVLFT
                     GATTGIVYVVGKTAHDAGCFHVGISPGTNELEHVELYKLPLDACDTIVYTGFGLKGRN
                     VVLVRSCDVVLFIAGAMGSLNEFTIAHDEGKIIGCLTGTGGVADESEYLLQKFGKRVT
                     TSRVFESDDPSELLDKCLQST"
     gene            complement(1001..2365)
                     /locus_tag="VFO72_07530"
     CDS             complement(1001..2365)
                     /locus_tag="VFO72_07530"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9479176.1"
                     /translation="MRLCFIGGSVASSSGMAEQTAETTNLPRYEPVGYIDLLRRNRAF
                     RQLWLGQVVSQMGDWFNTIALYTIILNLTGSGRDVGLLLVARFVPSFLFGPLSGVIAD
                     RFSRQQIMIVSDLLRALVVLGFLFVRSADQLWMVYLLTVLQLALSTFFEPAKTAAIPS
                     IVEDRELVAANAISSVTWSVMLTLGAAIGGLITGWFGTDVAFVLDAATYLLSAALIAS
                     IRVQKRRKRERQKLTLGRALGITQTLEGIRYVKDRPRVLALLLVKPAWGLGGGILTLL
                     AVFGERVFPVGRSAATGIGVLFAARGIGTAVGPIVARRMAGELDSRMQAWIGIAFLIG
                     GAFYIAFGSATSFVFALIVLGVAHCGGSILWVFSTVLLQRTVVDNFRGRVFAAELALL
                     TLTMAASNYATGELLDRFQISPRLVTIGIGVFFLLPGLAWFLTQRWWDRDVRNLMLCE
                     LKSE"
BASE COUNT          595 a          751 c          669 g          462 t
ORIGIN      
        1 gcggtcgttc cggtccggtt ttggaatcca ctcactccag ggaatgttca acgctatctt
       61 tatctgtgac ggcgcaagct tggaagcgac agtcagtccg gaggaaaaat cccaatccgt
      121 ctgcctgcct tcggcagcga gcacaatccc caaatcgacc cactctcccg tcggcgcagc
      181 ctcaaactcg aaatagcgcg acaaattctc cggatttggc gcgacaaaga tctcgcacac
      241 gtcacggtcc cacagtccca gagttttgcg atccgttttc ggttctgccg atacgaccag
      301 cggttcgtgt tggttaccaa caaaacgtac atgcagagcc tcgtcagacc agcagattcg
      361 cgcttcagcg tggcgctcga ccggggcagg cgcaccggac cagagatgag cgatggtaac
      421 cggcgcacac gtgtgccaca cctcgttctc aaaatcgccg gtggctatcg gtgtacctat
      481 gtactctgca aacatttgtc gagtagttcg ctcggatcgt cgctttcaaa cacacggctc
      541 gttgtcacgc gtttaccgaa cttctgtagc aaatactccg actcgtccgc cacgccgccc
      601 gtgcccgtca aacatccgat aatctttcct tcgtcatgcg cgatcgtaaa ctcgttcagc
      661 gaacccatcg ctccggcaat aaacaggaca acgtcacacg aacgcaccag caccacgttt
      721 cggcccttca ggccaaagcc cgtgtagacg atcgtgtcgc atgcatccag cggcaacttg
      781 taaagttcaa cgtgctcgag ttcgttggtg ccaggcgaga tgccgacgtg gaaacaaccc
      841 gcatcgtgag cggtttttcc aacgacgtaa acaatgcccg tcgttgcgcc agtgaagagc
      901 actacgttgc ggctcgcgat ctcgcggccc agtagctctg ccttttcgac cagcactttg
      961 cccgtttcga gccgtgtgct gtccggcgcc gccgagccca tcactccgat tttaattcgc
     1021 ataacatcag gttgcggacg tcacgatccc accagcgctg agtcagaaac cacgccagcc
     1081 caggcaacag aaaaaagact ccgataccaa tcgtaaccag acgtggcgag atctgaaatc
     1141 gatcgagcag ttcgcccgtc gcgtagtttg acgcagccat tgtcagtgtg agtaaagcca
     1201 actccgctgc aaagacgcgg ccgcgaaagt tatctaccac cgtgcgttgc agcaacaccg
     1261 tcgagaaaac ccagagaata ctaccgccgc agtgagcgac tcccagcact ataagcgcga
     1321 acacaaaact ggttgcactg ccaaaagcga tgtagaaggc gccgccgatc aaaaaagcaa
     1381 tgccaatcca ggcctgcatc cggctgtcga gctcgccggc catgcgacga gccacgatgg
     1441 ggccgacggc cgttccgata ccacgcgccg cgaacagcac gcctatgccg gtcgcagcac
     1501 tcctgccgac ggggaagact cgttctccaa acacagctaa caacgtgagt attccaccgc
     1561 cgagtcccca ggcgggtttg accagtaaca acgcgagcac acgtgggcga tccttaacgt
     1621 agcgaatacc ttcgagtgtt tgcgtgatgc cgagcgcgcg gcccaaagtc aacttttgcc
     1681 gctcccgttt acggcgtttt tgcacgcgaa tgctggcgat cagtgcggct gacaaaagat
     1741 acgtagcggc gtcaagaaca aaagcgacgt cagtgccaaa ccagcccgtt atcaagccgc
     1801 caatcgccgc gccgagcgtg agcatcaccg accacgtcac cgacgaaatg gcattagccg
     1861 cgaccagttc gcgatcttca acaatcgacg gaatcgccgc ggtcttggcc ggctcgaaga
     1921 acgtcgaaag cgctagttgc aggacggtta gcaggtagac catccagagt tgatccgcgc
     1981 tgcggacgaa caaaaatccg agtaccacga gcgcgcgcaa caggtccgag acgatcatta
     2041 tttgttggcg gctgaagcgg tctgcgatga cgcctgacaa cgggccaaac agaaaactcg
     2101 gaacgaaacg cgcgacgaga aggagcccta cgtcgcggcc cgagccggtc aagttgagga
     2161 tgatcgtgta gagcgcgatc gtgttgaacc agtcgcccat ctgactgacg acctgaccga
     2221 gccacagttg ccggaaggcg cgattgcggc ggagcagatc gatgtagccg actggttcat
     2281 accgtggcag gttcgttgtc tcggcggttt gttcggccat tccggatgag cttgccacag
     2341 aaccaccgat aaagcacaag cgcattgtga cgcaagctga ccgaagtttt cagtctgacc
     2401 tgtcgaattg aaagaagcgc gcgagagagt gcgaattttc gaggaaagcg acgcggcaca
     2461 gcagttgtga agaggca
//
LOCUS       DASTEY010000425         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1607874, whole genome shotgun sequence.
ACCESSION   DASTEY010000425 DASTEY010000000
VERSION     DASTEY010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607874"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VFO72_07535"
     CDS             complement(<1..1492)
                     /locus_tag="VFO72_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9479177.1"
                     /translation="MTERRHAPVATLVILLATVLGIGHLVAEAQQSRAKKAPVAKSIA
                     SPEELARAVTIYRDTYGVPHVFGRTDASTVFGFAYAQAEDNFWRVEENFIQALGRASE
                     LYGEKSLDEDRLNRALEIPKLAREEYSRLDPHMRSLCDAFAAGFNFYLARRPEVHPRL
                     LMKIEPWYTLAFIRYNYYQNGFARDRSLRRTTLQTAELETDLKPHTGSNGWVVGPTKS
                     ASGHTLLFINPHLPFFGSGQVYEGHLHSDEGWNFTGYTRFGFPLPYVGHNENGGWVST
                     DNAADLTDVYIETFDDPARPLAYKYGRGYRLATERVEEIKVKTSSGIETRRFNMRKTH
                     HGPIVASRDGKPLALKMAKLEADGWLREWYDMTKAQSLAALKKAMTPLNMLFGNVMYA
                     DRQGNTWYLYNGAVPRRNARFDWTRPVDGSDPATEWRGFHTIDELPQLTNPRTGWMQN
                     CNTTPFLLTSEGNPDPKRFPKYMVQEGDNPRGAIAREILSGKEKFAF"
     gene            complement(1496..1783)
                     /locus_tag="VFO72_07540"
     CDS             complement(1496..1783)
                     /locus_tag="VFO72_07540"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9479178.1"
                     /translation="MFHSDQHANIRVNKTLDRWLRLIVVTPNMHKVHHSREQIETDTN
                     YSNIFSVWDRIFGTYTPGVNFGRVRYGLDGFDDRDKQTLSALLKLPFSVNT"
     gene            1912..3342
                     /locus_tag="VFO72_07545"
     CDS             1912..3342
                     /locus_tag="VFO72_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479179.1"
                     /translation="MKHSMASLFLLLSLIPATVLAQATGQGTRKKRQPSVSRSQNPKI
                     CQGVAIPDGYVIVGYVTTPLCPHGAYVLKKQDSQSSNAVANDRRETPPPQTVKSRPRR
                     VRSDADVASVAGTTGSRPPTLSGNTAIGAPVSAASESSPTSDVEEVSEGDVVRIDTSF
                     VTVPVSVLDRQGRFVSDLKREDFRVFENGVEQPIAYFEPTEKPFTVALLLDTSASTRF
                     KLWEIKEAAITFAKQLRPQDRVLVVAFSDQVLLLTEATSDLQVVSTVIEANATAGSST
                     RLYDAVNLVVNERLNKMPGRKAIVLFTDGVDTSSYLATYQSTLREAEELDALIYPIQY
                     DTTDYMRAMQNGAGAVTVITTTNNWPFGGSSSARTVYGGPTNGAPPPGARKVDYDRAN
                     EYLHAMAERTGGRLYKANDKAQLAQAFTSIAEELRRQYSLGYYPRTGTDQTDQRRLIK
                     VSVQRQNVAVRARGSYMRRTGTNVGP"
     gene            complement(3379..>3763)
                     /locus_tag="VFO72_07550"
     CDS             complement(3379..>3763)
                     /locus_tag="VFO72_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET9479180.1"
                     /translation="APSFWLFIGPDALYWGPRHVAKLWGAKEIYITENGCSSSDEPAA
                     DGRVYDTDRVTFLRSYLTQLRRATAEKIPVRGYFLWSLTDNFEWADGYTNRFGIHYVD
                     YATQKRTPKLSADFYRELIARNALV"
BASE COUNT          850 a         1022 c         1067 g          824 t
ORIGIN      
        1 cgaaagcgaa cttttcttta ccggaaagga tctctcgggc gatcgcgccg cgcggattat
       61 cgccctcctg caccatgtat tttggaaagc gcttcggatc cggattgcct tcactcgtca
      121 aaaggaaagg cgttgtgttg cagttctgca tccatccggt cctgggattt gtaagctgtg
      181 gcaactcatc tatcgtgtga aagccccgcc actctgtcgc cggatcgctt ccgtccaccg
      241 gtctggtcca gtcaaaacgc gcattgcgcc gcggtaccgc gccgttgtag agataccacg
      301 tgtttccctg gcgatcggcg tacatgacat tgccaaacag catgttgagc ggcgtcatgg
      361 cttttttcaa cgcagcgagc gactgcgctt tggtcatgtc ataccactcc cgaagccaac
      421 catcggcctc gagctttgcc atcttgagcg cgagcggttt gccgtcgcgc gatgcgacga
      481 ttggaccatg atgcgtcttg cgcatgttga aacggcgtgt ttcgatgccg cttgaagtct
      541 ttactttgat ttcttcgacg cgctcagtcg ccaggcgata accgcgtcca tacttgtagg
      601 caagtggacg agcaggatcg tcgaaggtct caatgtaaac gtctgtgaga tcggccgcgt
      661 tgtcagtgct aacccaaccg ccgttttcat tgtgtccaac gtagggcaac ggaaatccga
      721 atcgtgtgta accggtgaag ttccagccct cgtcgctatg aaggtggccc tcgtaaacct
      781 gaccgctgcc gaagaatggc aagtgaggat tgatgaaaag caacgtgtgg ccactcgcac
      841 tcttcgttgg accaacaacc cagccgtttg agccggtgtg tggtttaagg tcggtttcga
      901 gttcagcggt ttgcagcgtg gtgcgtcgca ggcttctatc tcgcgcgaag ccgttctgat
      961 agtagttgta gcgaataaaa gcgagcgtgt accacggctc tattttcatc agcagtcgag
     1021 ggtgtacttc gggacggcgc gcgaggtaga agttgaaacc ggcagcaaaa gcgtcgcaca
     1081 aggaacgcat gtgcggatcg agtcgagagt attcctcgcg cgccagtttc gggatttcca
     1141 acgctcgatt cagccgatcc tcatcgagac tcttttcacc gtataactcg gaggcccggc
     1201 cgagagcctg aatgaaattc tcttctacac gccagaagtt gtcctcggcc tgagcataag
     1261 caaagccgaa tacagtgctc gcgtctgtgc ggccgaacac gtgcggcaca ccgtaggtat
     1321 cgcgatagat cgttacggcg cgcgcgagtt cctccggtga tgcaatgctc tttgcaaccg
     1381 gagctttctt tgcgcgcgac tgctgtgcct ctgcgacgag gtggccaatg cccaacactg
     1441 tcgccaaaag gatcactaga gttgcgactg gtgcgtgtct tcgttcggtc atacgttatg
     1501 tattcaccga gaacggtagc tttaataacg cagatagcgt ctgtttgtca cgatcgtcaa
     1561 acccgtccaa cccgtaacga acacgaccaa aattgacgcc aggtgtgtaa gtgccgaaga
     1621 tccgatccca aacagagaag atgttggaat agtttgtgtc tgtttcgatc tgttcgcgcg
     1681 aatggtgcac cttgtgcatg ttcggcgtga cgacaatcag acgtaaccag cgatcgagag
     1741 ttttgttgac gcggatgttg gcgtgttggt ctgagtgaaa cactccggga ccctggtttt
     1801 cgttctcaca gtcagggagc actacgcttg agggcttaag gtccaacggg aaatggacta
     1861 taatcccggc actcgaaata ggcagcagca aatcagtaga ggtacgcgac tatgaagcac
     1921 tcgatggcct ctctctttct cttactttcc cttatacccg cgactgtttt ggcccaggcc
     1981 acgggccagg gaaccaggaa aaaacgccaa ccttcggtat cgcgcagcca aaatccaaaa
     2041 atctgccagg gtgtcgccat tcctgacggc tacgtgattg ttggttacgt aacgacaccg
     2101 ctctgtccgc acggagcgta cgtgctaaag aaacaggatt cgcagagttc gaacgcagtc
     2161 gcgaatgatc ggcgtgaaac cccgccgccg cagacggtaa agtcacggcc gcgccgggtt
     2221 cgcagtgacg ccgacgtcgc ttctgtggcc ggcacgaccg gctcgcgtcc gcccacactt
     2281 tcgggcaaca cggcgattgg cgcgccggtg agcgccgcgt ctgaaagtag tcccacgagt
     2341 gacgtcgaag aggtatcgga aggtgacgta gttcgcatcg acacgtcttt cgtcactgtt
     2401 ccggtgagtg ttctcgaccg gcagggtcgc tttgtttcgg acctgaagcg agaagacttt
     2461 cgtgtgtttg agaatggcgt cgagcagcct atagcgtatt tcgagccaac ggagaaacca
     2521 tttactgttg ctcttttgtt ggacacgtcg gcttcgacac gttttaagtt gtgggagatc
     2581 aaagaagccg cgattacgtt tgccaaacaa ctgcgtccgc aagatcgcgt gctggttgta
     2641 gcgttcagcg atcaggtgtt gttactgacc gaagccacga gcgacttaca ggttgtcagc
     2701 acagtaatcg aagcgaatgc gacggcgggg agttccacgc gactgtacga tgcggtgaat
     2761 ctcgtagtta atgaacgcct caacaagatg ccgggtcgaa aagcgatcgt gctgttcacc
     2821 gatggcgtcg acacttccag ctatctcgca acgtatcaaa gcactctgcg cgaggcggaa
     2881 gagttggatg cgctgatcta tccgattcag tacgacacga ccgattacat gcgcgctatg
     2941 caaaatggag caggagcagt gacggtcata acgaccacga ataactggcc gtttggggga
     3001 agctcctctg cacgaacggt gtatggtggt ccaaccaacg gcgctccgcc accgggtgcg
     3061 aggaaggtcg actatgaccg ggccaatgaa tatcttcacg caatggctga aaggacggga
     3121 ggacggcttt acaaagcgaa cgataaggct cagctcgcac aggcgttcac cagcatcgct
     3181 gaagagcttc gacgtcaata tagccttggg tattatccac ggacgggcac ggatcaaacg
     3241 gaccagcgtc gcctgatcaa agtgagcgtt cagcggcaga acgttgcggt gcgagcgcgc
     3301 ggcagctaca tgcgccgcac gggaacaaac gtcggtccgt gaccgccgaa ggcttagacc
     3361 tactcgcgtg gcacgcaatt agaccaacgc gttgcgcgcg atgagttcgc ggtagaagtc
     3421 ggcgctcagc ttcggggtgc gcttctgggt ggcataatca acgtagtgaa tgccaaaacg
     3481 attcgtgtaa ccatcagccc actcaaaatt gtccgtcaaa ctccacaaga aatatccacg
     3541 caccggtatc ttctccgctg tcgcacggcg caactgcgtc agatagctgc gcaaaaacgt
     3601 aacacgatcg gtgtcgtaga cgcgcccgtc tgcggccggc tcgtcagaag aagagcaacc
     3661 attctccgtg atgtaaatct ccttcgcacc ccagagcttc gcaacgtgac gcggtcccca
     3721 atagagcgca tccggcccaa tgaaaagcca aaacgaaggc gcg
//
LOCUS       DASTEY010000426         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1525947, whole genome shotgun sequence.
ACCESSION   DASTEY010000426 DASTEY010000000
VERSION     DASTEY010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525947"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1359
                     /locus_tag="VFO72_07555"
     CDS             178..1359
                     /locus_tag="VFO72_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479181.1"
                     /translation="MRASIGGMMQDSRVPTMTLGFEEKLRRPAFLLGVLAFFLFVSLS
                     APAVQAQSVKDLPPPPPLWKPKPTPTPKPPEQEVLDVVRVTSNLVMVPVSVTDPQGNA
                     VQGLTKTDFRLVEEGKQQEISEIGDPEQVPLAIALLFDVSSSVSQKGFFASQQNAAAS
                     FLKLVMKPADKAAIFTITDKATVIQPLASAETSAAKMLTIPAATTPVPTAFYDTVIAA
                     AKYLTDNAPSNHRRVIVVLSDGDDNFSEQIRDLSIAEARATQAGQPTFASTRAGLQER
                     HRRAVQQVQQAVQKADVIFYSVNPGGPSVRMNQISMRAELGMEAIAETTGGTAFVPES
                     DKDLDRVFRQVAAELRGQYLLQYYANAEAPANQFRRIQVTVPARGGVRIRARQGYYPK
                     K"
     gene            complement(1471..1992)
                     /locus_tag="VFO72_07560"
     CDS             complement(1471..1992)
                     /locus_tag="VFO72_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479182.1"
                     /translation="MLKRIAIALLILIFVAPTAALGQTRRRTPRSRPAQPSTQRVSQV
                     RTAGATRVADQIKLMTRFVYLLGGIANGIATVDEAARRNEASPELLQRNQQNKVTVRT
                     SIQGFREGLDKLEIDFRATPDLQPYYIKLAGSAAGAAKAEEQAAAGQFDAAGRTLLTV
                     INRLTDVLVVMRQ"
     gene            2098..2769
                     /gene="lipB"
                     /locus_tag="VFO72_07565"
     CDS             2098..2769
                     /gene="lipB"
                     /locus_tag="VFO72_07565"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET9479183.1"
                     /translation="MKKERKLLVQRLGLIDYPSGLALQKEIEQAVKLRTQPDTLLLLE
                     HPHTLTIGRRGDSSAVLLDAKQLLKRNVTVFETNRGGKVTYHGLGQVVGYPIVNLSPD
                     REDVHRYMRDLEEVLIRAMADFGIDAFRIDSLTGVYTARGKIAAIGVHIARWVTTHGF
                     ALNVNTDLSYFDFIIACEGEPVTSMEQLLGSETPLSEVEDRIVARFAEVFAMRVEMDS
                     CLQPT"
     gene            2751..>3534
                     /locus_tag="VFO72_07570"
     CDS             2751..>3534
                     /locus_tag="VFO72_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET9479184.1"
                     /translation="MLTTDLIHEARARISSRIHRTPVVTSRQFNEVAGKQVFFKCENL
                     QRAGAFKIRGATNKIQSLTDEEKSRGIVAFSSGNHAQAVALAGREAGVRVLVAMPDDA
                     PRSKVAATRDYGAEIIFYDRHKADREKFALDLAERERLVMVPPYDDYLILAGQATCGL
                     ELLEEVPDLECVLTPCSGGGLFAGVATAARMLNPRIRCFPVEPATADDTRQSFLKGER
                     VSIPPPPTIADGLRVQIPGTLTFPIVQQLAEDVLTVSDEEIIE"
BASE COUNT          787 a          948 c         1022 g          777 t
ORIGIN      
        1 aagctgcttg tgaacaaggg acttacttct cccgcaccgg caatgaaccc gggtacatcc
       61 ggtaactaaa gttctaacgc ttctgtggag atgcactgct ggtgcaactc caccgatgca
      121 agtgggcacc aaagagtcgt ggatcatgcc gtcagaaaag cggtaggata agaacacatg
      181 cgagccagca ttggcggcat gatgcaagac tcgagggtgc caacgatgac tttggggttt
      241 gaggaaaaac ttcgacggcc ggcattcctg ctgggagtgc tggccttctt tctttttgtt
      301 tctctttccg cgccggcggt gcaggcgcaa agcgtcaaag acttgccgcc accgccacct
      361 ctctggaagc ctaaacccac gcctacgcca aaaccacccg agcaggaggt gctcgacgtg
      421 gtgcgagtca cttcaaacct cgtgatggtc ccggtttccg taactgaccc gcagggtaat
      481 gccgttcaag gactgacaaa aactgatttc cgtctcgtcg aagaagggaa gcaacaggag
      541 atcagcgaga ttggtgaccc cgagcaagtg ccgctcgcga tcgcgctgtt gtttgacgtt
      601 tccagcagcg tcagtcaaaa aggtttcttt gcgtcgcaac agaatgccgc agcgtcgttt
      661 ttgaagctgg tgatgaagcc cgcagataaa gcggcgatct tcaccatcac cgacaaggcg
      721 acggtgatcc aaccgcttgc ttccgccgaa acttcagccg ccaaaatgtt gactattcca
      781 gcggcaacga cgccggtgcc gactgcgttc tacgatactg tgatagccgc ggcgaagtat
      841 ttgactgaca acgcgccgag taatcatcgg cgagtaatcg tggtcctttc agacggtgac
      901 gataatttca gcgaacagat tcgcgacttg tcgatcgccg aagcgcgtgc gactcaggca
      961 gggcagccga cgtttgcgag cacacgcgca ggtttgcagg aacgacaccg ccgcgcagtg
     1021 cagcaggtcc aacaggctgt gcaaaaggcc gatgtgatct tttactcggt gaatcccggt
     1081 ggtccgtcag tccggatgaa tcagatcagt atgcgcgctg aactggggat ggaagcgatt
     1141 gcggagacga ccggaggaac cgcgtttgtg ccggagtcgg ataaagattt agatcgagtg
     1201 tttcgacagg tggcggcgga gttacgtggg caatacctgc ttcagtacta cgcaaacgca
     1261 gaagcacccg cgaatcaatt tcggcggata caagtaaccg taccggcacg tggcggcgtg
     1321 cgcattcgcg cgcgccaggg ctactatccg aagaaatgaa ggaattgccg attttcgatt
     1381 gccaattgcc gattgcaaat cgccgatttt tgcagtagct ctgcaagttg agttaaatcg
     1441 gcaatcggca atcgaaaatc ggcaatgtct ttactgccgc attactacca gtacgtcagt
     1501 caaccggtta atcaccgtca acaaggttcg tccggcagcg tcaaattggc ccgctgccgc
     1561 ttgctcttct gccttcgctg ctcccgcggc tgagccggcg agcttgatgt aataaggctg
     1621 caagtccggc gtagcgcgaa aatctatttc cagcttatcg agaccttcac gaaaaccctg
     1681 aatgctcgtt cgcaccgtca ccttgttctg ctgatttctc tgtagcagct ccggcgacgc
     1741 ctcgtttctt cgcgcggcct cgtcgactgt ggcaatgccg ttcgcgatgc cgccaagcag
     1801 gtagacaaac ctggtcatca gtttgatctg atcggcgacg cgggtagcgc cggcggtgcg
     1861 cacttgcgac acgcgctggg ttgatggctg agccggtctc gacctgggcg tccgccttcg
     1921 cgtttggccc aacgcggcgg tgggcgcgac aaaaattagt atcagcaaag caatggcgat
     1981 gcgtttcaac atgattctcc tcttcaacta tccgggaatt acggattgta ccaaagaccg
     2041 aggcggctgc tagaatgacc tcacaagact gacgccgaaa gactactccg tggttgcatg
     2101 aaaaaagaaa gaaagcttct ggtccaaagg ctcggcctca tcgattaccc ctcaggtctc
     2161 gcgcttcaaa aggagattga acaggcagtc aaactccgta cacaaccgga cacgcttttg
     2221 ttgctcgaac atccgcacac gctgacgatc ggccgtcgtg gcgacagttc ggcagtgctg
     2281 ctcgacgcca aacagttgct gaagcgaaac gtgaccgtgt ttgaaaccaa ccgcggcgga
     2341 aaagttacgt atcatggcct gggccaggtc gtgggttatc caatcgtcaa cctcagtccg
     2401 gatcgcgaag acgtgcatcg ctacatgcgc gatctggaag aggtgctgat tcgagcaatg
     2461 gcggatttcg gcattgatgc atttcgaata gatagcctca cgggagttta caccgcgcgt
     2521 ggcaagatcg cggctattgg ggttcacatt gcacggtggg tcacgacgca cgggtttgct
     2581 ctcaacgtca atacagatct cagctacttt gatttcatta tcgcctgcga aggtgaacca
     2641 gtgacttcga tggagcagtt gcttggttcc gaaactccgt tgagcgaagt tgaagatcgc
     2701 atcgtggcgc ggttcgccga agtctttgcg atgcgagttg aaatggactc atgcttacaa
     2761 ccgacctgat tcacgaagcg cgcgcgcgca tctcgtcacg cattcatcgt acgccggttg
     2821 tcacttcgcg gcagtttaac gaggttgccg gaaaacaagt cttcttcaaa tgcgaaaacc
     2881 tgcaacgcgc aggcgcgttc aagattcgtg gcgccactaa caagattcag tcgttgacag
     2941 acgaggaaaa gagccgcggc atcgttgcgt tctcttccgg caatcacgcg caggcggttg
     3001 cgcttgctgg gcgcgaagcg ggcgtgcgcg tcctggttgc aatgcctgac gatgcgccgc
     3061 gttcgaaagt cgcggcgaca cgcgattacg gcgctgagat catcttttac gatcgtcaca
     3121 aggcagaccg tgaaaagttt gctctcgatc tcgccgagcg cgaacggttg gtgatggttc
     3181 cgccgtatga cgattatttg atactcgcgg gccaggcgac ctgcggtctt gaattgctgg
     3241 aagaggtgcc ggatctcgag tgcgtgctga cgccgtgcag tggtggcggt ttatttgccg
     3301 gcgttgcgac tgcggcgaga atgttgaatc cgcgcatccg ttgttttccg gttgagcctg
     3361 cgactgctga cgacacgcgt cagtcgtttc tgaaaggcga gcgcgtttcg attccgccgc
     3421 cgccgacgat agctgacgga ttgcgagttc aaattccggg aacgctgacg tttccaatcg
     3481 tgcagcagtt ggcggaagat gtgttgacgg tttccgatga agagatcatc gaga
//
LOCUS       DASTEY010000427         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_174349, whole genome shotgun sequence.
ACCESSION   DASTEY010000427 DASTEY010000000
VERSION     DASTEY010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174349"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFO72_07575"
     CDS             complement(<1..294)
                     /locus_tag="VFO72_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9479185.1"
                     /translation="MLKVEHISKEYPTPSGPLQILSDVSLELPTGSAVAIMGPSGSGK
                     STLLYVLGALEPPTSGTVILDNQNPYLLDAKQLAAFRNQRIGFVFQDHCLLPQC"
     gene            complement(287..1594)
                     /locus_tag="VFO72_07580"
     CDS             complement(287..1594)
                     /locus_tag="VFO72_07580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9479186.1"
                     /translation="MRILYLTGGAGRMYCGSCLRDNAMATELLARGHDVLLLPVYTPT
                     FTDEPNVSQDHVVLGGISAYLEQYVPLFRKTPSWLDRLWDSKAVLSLASRRSISTNPK
                     MLGEMTVSVLKGEEGFQRKEIHKLIDWVKQLPVPDVINLPYTLLIALAKPLKQALNRP
                     IFCTLQGEDLFLDGLQEPYRTEAFELIRRQLDQVDLFLSVSEYYAEFMPRYLGIPVEK
                     IRVVPLGINPQGFELRERNTSGSFTVGYFARIAPEKGLHVLAEAYRILRQSGDLPQGR
                     LEVAGYMAADCKPYLSEIEARLRQADLAHEFHYRGAFDRAEKIAFLRGLDVMSIPATY
                     DEPKGVSLLEAMACGVPLVQPRRGGFTEIIEKTGGGLLVKPDDPKALADGILQVYQNT
                     ELATKLSANGFRGVRQHYTAAHMADKLLNAFQSVLSVAENKVA"
     gene            complement(1603..>2014)
                     /locus_tag="VFO72_07585"
     CDS             complement(1603..>2014)
                     /locus_tag="VFO72_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479187.1"
                     /translation="NSIIDQGLLEPGARDVTASYRQNERLIVWNDCSYLNPTLIRCEA
                     DHSLANREFLFPFATVLQVPQDQILSRLGPTLVVTAITKDAALIRTLVNSPHIDRLNI
                     GPLPTNQVSWDQPHEGNLFEHLYGRRAFQDAVAV"
BASE COUNT          454 a          551 c          575 g          434 t
ORIGIN      
        1 acactgcggc agaagacagt ggtcctgaaa cacgaagccg atacgttgat tgcgaaaagc
       61 agcgagttgc ttcgcgtcga gtagatacgg attctgattg tcgagaataa cggttccgct
      121 cgtcggtggt tcgagggcgc cgagcacgta cagcaatgtg ctcttgccgc tgccggaagg
      181 tcccatgatg gccacagcag atccggtcgg aagttcgagc gaaacgtcac tcaaaatctg
      241 gagtggtcca ctgggagtcg gatactcctt tgagatgtgt tcaaccttaa gcaaccttat
      301 tctccgccac ggataacact gattgaaaag cgttcaggag tttatcggcc atgtgggcgg
      361 cggtatagtg ctggcgcaca ccgcggaaac cgttcgcgct cagcttcgtc gcgagttccg
      421 tgttttggta aacctgaaga attccatcgg cgagtgcttt cggatcatcg ggctttacca
      481 gcagaccgcc gcctgtcttt tcgataattt ccgtgaaccc gccacgacgt ggttggacca
      541 gcggcacgcc gcacgccatt gcctcgagca gcgacacgcc tttgggctca tcgtaagtcg
      601 ctggaatcga catcacgtcg agtccgcgca gaaatgcgat cttctctgcg cgatcgaacg
      661 cgccgcggta atgaaactcg tgtgcgagat ctgcttgtct cagacgtgct tcgatttcgc
      721 tgagatacgg tttgcagtcg gccgccatgt agcccgcaac ttccaaacgg ccctgcggta
      781 gatcgccgga ctgtcgcagg atgcgatacg cttccgccag cacgtgcaaa cccttttccg
      841 gcgcgatgcg cgcgaagtaa cccacggtga atgatccgct ggtgttccgc tctcgtaatt
      901 caaaaccttg tggattgata cccagcggaa ccacgcgaat cttctcaacg ggaataccaa
      961 ggtaccgcgg catgaactcc gcatagtact cgctaacaga aagaaacaga tcgacctggt
     1021 ccagttgtcg ccgaataagc tcgaacgctt cggtgcgata cggctcctgt agtccatcca
     1081 aaaacaggtc ttcgccttga agcgtacaaa aaatcggtct attgagtgct tgcttgagcg
     1141 gcttggcgag tgcaatcaga agcgtgtaag ggagattgat gacatcgggg accggcagtt
     1201 gcttcaccca gtcaattaac ttgtggatct ccttgcgctg aaagccctct tcacccttta
     1261 acaccgacac cgtcatctcc ccgagcattt tcgggttggt cgagatcgac cggcgcgaag
     1321 ccagactcaa aaccgccttg gagtcccaca atcgatcgag ccagctcgga gtcttgcgga
     1381 agagcggaac gtactgttcg agataagcac tgatgcctcc caagaccaca tggtcctggc
     1441 tgacgttagg ttcgtcagtg aacgtgggcg tatagaccgg caacagcagc acgtcgtgcc
     1501 cgcgcgcgag cagttctgtc gccatggcgt tgtcgcgcag gcaactgccg cagtacatac
     1561 ggccggctcc acctgtgaga tagagaattc tcacgcacct cctcaaacag cgacggcgtc
     1621 ttgaaaagct cgccgcccgt aaagatgttc gaagagattg ccttcgtggg gttggtccca
     1681 actgacctgg ttagtgggca gtggaccaat attcaggcga tcgatatgcg gggagttgac
     1741 gagtgtgcgg atcagtgccg cgtctttagt aatggccgtg acgacaagag tcggacccag
     1801 gcgcgacaag atctgatcct gcgggacctg cagtaccgta gcaaacggaa acaggaattc
     1861 acggttcgcg agcgagtgat ccgcttcaca acggattaaa gtcggattca gataggaaca
     1921 gtcgttccag acgatgagtc gctcgttctg acggtacgag gcagtgacgt cgcgggcgcc
     1981 gggttccagc agtccttgat cgatgattga gttg
//
LOCUS       DASTEY010000428         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_602276, whole genome shotgun sequence.
ACCESSION   DASTEY010000428 DASTEY010000000
VERSION     DASTEY010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602276"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VFO72_07590"
     CDS             complement(<1..1191)
                     /locus_tag="VFO72_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9479188.1"
                     /translation="MQKPRLSFWQIWNMSFGFLGIQFGWGLQLANMSPIYKYLHAEES
                     SLPYLWLAGPITGLIVQPIIGSMSDRTWNRLGRRRPDFLTGAILASIALFFMPDSSVL
                     WMAAGLLWILDASINITMEPFRAFVADKLPEEQRTLGFVMQSFFIGVGQTLANGLPYL
                     FSALGVVGVMATGIPLSVDYAFKIGAIVFLVAVLWTVVTTKEYPPENLEEFERMKRET
                     KGVLAGFKEIFSSVTEMPPTMKQLAVVQFFTWFALPCMWQFFSLAIARHVFLAPDESS
                     PRFAEATEWGGLCFAVYNVVCFVIAFLLPAFANATSRKTVHMIALVCGGVGLISVFFI
                     GNKWMLFASMAGVGIAWASILSMPYVMLSTAVPPARMGVYMGVFNLFIVIPQIVMSLI
                     VPSIF"
     gene            complement(1198..>2150)
                     /locus_tag="VFO72_07595"
     CDS             complement(1198..>2150)
                     /locus_tag="VFO72_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9479189.1"
                     /translation="LNQHDPEVSRYLIQNTLWWIGTTGLDGIRMDTWQYVPNSFWREW
                     NRALKREFPNFRVVGEVKDGDVVHTSFFQGGRVRFDGVDSGLDSLLDFPLFYPIRHAF
                     AEGKPIDEIPKTLAKDYLYTNSEILVTLLGGHDDGRFMSEKGATIAGLKLANAFVLTT
                     RGVPQLYYGDEIAMEGGDEPTTRGDFPGGFPGDKRNAFTAAGRTKEEQELFEYIRWLI
                     YRRREFPVLQRGALINLYVAEQQYAYARVLPDTAAIVVFNNDDKAAEVEFDVTRAGLK
                     EGVTILNLQTVNRDVLRGGKMRVSLPKRSVAIFVSWLNKG"
BASE COUNT          554 a          632 c          535 g          429 t
ORIGIN      
        1 gaaaatactc ggcacgatga gcgacatcac aatctgcgga atcacgatga acagattgaa
       61 cacacccatg taaacaccca tgcgcgccgg cggcactgcc gtcgacaaca tcacatacgg
      121 catcgacaaa atcgacgccc acgcgatccc gacaccagcc atgctcgcaa acaacatcca
      181 tttatttcct atgaaaaaga ctgagatcaa tccgacacca ccgcacacga gcgcgatcat
      241 gtgaacggtc ttgcgactcg tcgcgttcgc aaacgccggc aacaaaaagg cgatgacaaa
      301 gcacaccacg ttgtagacag cgaaacacag accaccccat tctgtcgctt ctgcaaaacg
      361 cggtgacgat tcatcgggag caagaaagac gtgccgtgct atcgctaatg aaaagaactg
      421 ccacatgcaa ggcaatgcaa accaggtgaa gaactggacc accgcgagtt gcttcatcgt
      481 cgggggcatc tcggttacag acgaaaagat ctctttaaat ccggcaagaa ctcccttcgt
      541 ctctcgtttc atacgctcga actcttccag gttctccggc gggtactctt tcgtagtaac
      601 cacagtccac aagaccgcta cgagaaaaac aatcgctccg atcttgaaag cgtaatcaac
      661 tgacagcgga atgcccgtcg ccatcactcc gacaacaccc agcgctgaga acaggtaggg
      721 caagccattg gcaagcgtct gaccgacgcc gatgaaaaaa ctctgcatca caaatccgag
      781 cgtgcgttgt tcttcgggca acttatccgc gacgaacgcg cgaaacggtt ccatcgtgat
      841 gttgatactg gcgtcgagga tccaaagcag tccggcagcc atccacagca cagacgaatc
      901 aggcatgaag aacagggcga tactggcgag aatcgcgccg gtgagaaaat cagggcggcg
      961 ccgtccgagc cggttccacg tgcgatcgct catcgatccg atgatcggtt gaacgatcaa
     1021 acccgtgatt ggaccagcga gccataggta aggtagcgag ctctcctcgg cgtgcaaata
     1081 tttataaatc gggctcatgt tcgccagctg caaaccccag ccaaactgaa tcccgaggaa
     1141 cccgaaactc atgttccaga tctgccagaa gctcaagcgt ggtttctgca tcggaagcta
     1201 tcctttgttt agccagctga cgaagattgc tactgaacgc tttggtaaac tgacgcgcat
     1261 cttgccgccg cgaagcacat cgcggttgac agtctgcaag ttgagtatcg tcacaccttc
     1321 tttcaaaccc gcacgcgtaa cgtcaaactc aacctccgct gccttgtcgt cgttattgaa
     1381 gactactatc gcagcggtat ctggaagtac gcgcgcatat gcgtattgct gctcggcaac
     1441 atacagatta atcaacgcac cacgctgcag taccgggaac tcacgtcgcc tgtagatcag
     1501 ccagcgaatg tattcaaata gttcctgctc ttctttagtg cgacctgccg ctgtaaacgc
     1561 attacgcttg tcaccgggaa atccacccgg gaaatcgccg cgcgtggtcg gctcgtcccc
     1621 accttccatc gcgatctcgt cgccgtagta aagttgcggc acgccacgcg tagtcaacac
     1681 gaatgcattc gcgagcttga gtccggcgat cgtcgcgcct ttctcgctca tgaagcggcc
     1741 atcgtcgtgt ccgccgagca aggtgacgag gatctcagaa ttggtataga gatagtcttt
     1801 agcgagcgtc ttcggaattt cgtcaatcgg cttgccttca gcaaacgcgt ggcggatcgg
     1861 atagaagagt ggaaagtcga gcagcgaatc gagcccggaa tcaacgccgt caaagcgaac
     1921 tctgccgccc tgaaagaacg acgtatggac cacgtcgccg tccttcacct cgcccaccac
     1981 tctgaaattc gggaactcgc gctttaacgc cctgttccac tcacgccaga acgagttggg
     2041 cacgtattgc catgtgtcca tgcggatgcc gtcgaggccg gtggtgccaa tccaccacaa
     2101 cgtattttga atgagataac gcgagacctc ggggtcgtgc tggttcagat
//
LOCUS       DASTEY010000429         4353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_371701, whole genome shotgun sequence.
ACCESSION   DASTEY010000429 DASTEY010000000
VERSION     DASTEY010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371701"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..2088
                     /locus_tag="VFO72_07600"
     CDS             100..2088
                     /locus_tag="VFO72_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479190.1"
                     /translation="MSAAHVNVNRDSFGHVYMWSLVTAGAAIVLASLFVLPFRDLDPG
                     FVFLCLMVMASSLVAIKIPRVSGRITVADTFVFLTMLLYGGAAAILMSALEGVAATLI
                     ISKRPRTILFNSAILATSTFFTVAVVYWIAGPPAKIIDGDQRFTAKFFGAVCTMALVQ
                     YMANTVLIAIEKSSKIKESVWNTWRTYYLWTSVTYFAGASAAGIIAVLMKTYGFYAVV
                     ATVPIILIICFTYQTYLKNIEASVAQTEAARLHVQELSRYISELQRSEEAREQLLLRA
                     ERARAEAEAANRIKDEFLATLSHELRTPLTSLLGWSSVLREARRDERVLVQGLEAIDR
                     NARVQAQLIDDLLDVSRIVSGKLNLDVRPLDIASVTRAAINVVQPAAHAKGITLDYWA
                     QPGLGAISADSARLQQIIWNLLSNAVKFTPHGGKINVRVACDGSHAKVTVSDTGQGID
                     SEFLPRVFDRFRQADSSTTRSFGGLGLGLAIVRHLVELHGGTVSAESEGVGKGATFSA
                     SFPLLNDRAEPVAAMHSGEVLASELRSLDGLRVLLVDDEPETREIISTVVERTGAEVK
                     TCTSAREALSELVDWRPHVILSDIAMPDEDGYSFIGRVRSLPQNEGGGTPAAALTAYA
                     REEDRRQALAAGYQMHIAKPIGAGQLVTMIAKLAGREN"
     gene            2288..3373
                     /locus_tag="VFO72_07605"
     CDS             2288..3373
                     /locus_tag="VFO72_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9479191.1"
                     /translation="MPVGIGIVGAGFARTTQIPGFRDCMGAKVVAITSRHPERAAVVA
                     KEFGIEHVAADWQELVERNDVDLVSIVTPPSTHMEITLAALEQRKAVLCEKPMALNAG
                     EAARMVEKARSANVLALIDHELRFLNSRRTMRGMLQTGAIGTVRHCNYVFRSDYRGLA
                     DRGWDWWSDAAMGGGTLGAIGSHVIDSFRWLLSTEVSRVFGMLTTHIKERRDKSSGGM
                     RAVTTDDEAKLLFHFTDGPYTNGATGAAALSVVESGKYENRLEIYGTKGALMVEETGE
                     LWLSPAGSGTWRPVQVDQDHMAQGMRESSWSRGFTAFACRIVDAMRSGKKTVEDAASF
                     EDGHRVQVVLDAVRESNERGCWVDVGG"
     gene            3366..>4353
                     /locus_tag="VFO72_07610"
     CDS             3366..>4353
                     /locus_tag="VFO72_07610"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9479192.1"
                     /translation="MGKRTRVGVDTGGTFTDFVCEQDGEVHLFKVPSTPSDPSIAIRQ
                     GLDRICSEIPVQLTALEVVHGTTVGTNALLQRRGARTALITTRGFEDVLAIGRQARPE
                     LYNLNAVKPAPLVPDELRLGVAERVVASGEVIKPLEDAELPDIVDRLKRENVESVAVS
                     LLFSFLHPQHEEQIAKVIASLGVPVSISSRILPEYREYERTSTVVINAYLQPLMGRYL
                     ERLTKQKVQSSKLNLRVMQSSGGSISAEMAAVEPVRTILSGPSGGVVGALRAAHAAGL
                     KNIITFDMGGTSTDVALCDDGAIRTTNEASVAGLPVAISVMDIHTVGAGGGSI"
BASE COUNT          920 a         1182 c         1303 g          948 t
ORIGIN      
        1 ttctgactgc ctcgattatt agttttcgtt tggagtgcgt tgatctatcg acgcactcca
       61 atagtgctct ccacaccgac cagtcttctc gaggtaacaa tgagtgcggc acatgtaaat
      121 gtgaatcgag atagctttgg gcacgtgtat atgtggtcgc tggtaaccgc gggcgcagcg
      181 atcgtactcg cgtcactgtt tgtcttaccg tttcgtgacc tggatcccgg ctttgtcttt
      241 ctctgcctga tggtcatggc gagttcgctc gtcgcaatca aaatacccag agttagcggc
      301 cgcatcactg tggccgacac attcgttttc ctgacaatgt tgctgtacgg cggagccgca
      361 gcaattctta tgtcggcgct cgaaggtgtt gcggccactc tgattatcag caaacgcccg
      421 cgcacaatcc tgttcaactc tgccattctg gcgacttcta cgtttttcac agtcgctgtc
      481 gtgtattgga ttgccggtcc gccggcgaag atcatcgacg gcgaccaacg tttcaccgcg
      541 aagttctttg gcgcagtctg cacgatggcg ctggtccagt acatggcaaa caccgttttg
      601 atcgcgatcg agaagtcttc caagatcaaa gaatcagtct ggaacacctg gcgcacttat
      661 tatctgtgga cttccgtcac gtacttcgcg ggagcatcgg ccgccggcat cattgccgtc
      721 ttgatgaaga cctacggctt ctacgcggtg gtggcgacgg tgccgatcat cctgatcatc
      781 tgcttcacct accagacgta tctcaaaaac atcgaagcgt cggtggcgca gactgaggcc
      841 gcccggctcc acgtccagga gctttcgcga tatatcagcg aactgcagcg ttccgaagaa
      901 gctcgcgaac aacttttgct gcgagctgaa cgcgcgcgtg ctgaagcgga ggccgcgaat
      961 cgaatcaaag atgagttcct ggcaacgctt tcgcacgaat tgcgcacgcc gctcacttcg
     1021 ctgctcggtt ggtccagcgt cctgcgcgaa gcgcgtcgcg acgaaagagt actggtgcag
     1081 ggacttgaag caatcgatcg caatgcgcga gtgcaggcac aactgatcga tgatctgctc
     1141 gatgtctcgc gcattgtttc gggcaaactg aatctcgacg ttaggccgct cgatatcgct
     1201 tcagtgacgc gagccgcgat caatgtcgtg caaccggctg cccatgcgaa aggcatcacg
     1261 ttggactact gggcccaacc aggtttgggc gcaatttccg cagactcggc tcgtttgcag
     1321 cagatcatct ggaacttgtt atccaatgcc gtgaagttca cgccgcacgg cggcaagatc
     1381 aatgtgcgtg tggcgtgtga tggatcgcat gcgaaagtga cggtgagcga caccggccag
     1441 ggcatcgact cggagtttct gccgcgcgta ttcgatcgtt ttcgtcaggc tgacagttct
     1501 acgacgcgca gttttggtgg tctcggtttg ggattggcga tcgttcgtca ccttgtcgaa
     1561 ctgcacggcg gcacggtttc ggctgaaagc gagggcgttg gcaagggcgc tactttttcc
     1621 gcttcgtttc cgctgcttaa cgatcgtgcg gaaccggttg cggcgatgca cagcggcgag
     1681 gttcttgctt cggagttgcg atcgctcgac ggtttgcgcg tgttgctggt tgatgatgaa
     1741 ccggaaacgc gtgagatcat cagcacggtg gtcgagcgca ctggagccga agtaaaaaca
     1801 tgcacgtcgg cgcgcgaagc gctgtcagag ttggtcgact ggcggccgca tgtaattctg
     1861 agtgatatcg cgatgcctga cgaagatgga tacagcttta tcggcagagt cagatcgctt
     1921 ccgcagaatg agggcggcgg cacgccggcc gcggcactaa cagcctacgc gcgcgaagag
     1981 gatcgcaggc aggcgcttgc agcgggctat caaatgcaca tcgccaaacc gatcggcgcg
     2041 ggccaactcg ttacgatgat cgccaaactc gccggccgcg aaaactgagt cacaaagaag
     2101 cacaagaaac acaagtggcg ccctaaattg aattcttctc tgtgcctctg tgtctcggtg
     2161 gttatttcgt tccaggtaac aaccacagag acacagcaca cagagaaaac ctcaaatccg
     2221 gcattaagga aacacaaaga gttaccaaac cggctcatgc tgtggtaaga attagggcat
     2281 gaagaccatg ccggtaggaa ttggaatcgt cggggcgggg tttgcccgga cgacgcagat
     2341 cccgggtttt cgtgattgta tgggcgcgaa ggtggtggca atcacaagtc gccatcccga
     2401 acgtgccgcg gtagtcgcca aagagtttgg catcgaacat gtagcggccg attggcagga
     2461 gttggttgaa cgcaacgacg tcgatctggt cagcatcgtc actccgcctt ccactcacat
     2521 ggagatcacg ctcgcggcgc tcgagcagcg caaggccgtg ttgtgcgaaa agcccatggc
     2581 gctcaacgcc ggcgaagcgg cgcggatggt tgaaaaggcg cgctcggcca acgtgctcgc
     2641 gttgatcgat cacgagttgc ggtttttgaa cagccggcgc acgatgcggg gaatgttgca
     2701 aaccggagca atcggaaccg tgcgccattg caactacgtt ttccgttctg actatcgtgg
     2761 cctcgccgat cgcggatggg actggtggtc tgacgcggcg atgggcggcg gcaccctggg
     2821 cgcgatcggt tcgcacgtga tcgactcgtt tcgctggttg ttgagcacgg aggtctcacg
     2881 cgtcttcggc atgctcacaa cacacatcaa agaacgacgt gacaaatctt cgggcggcat
     2941 gcgcgctgtc accactgacg acgaagcaaa actgttgttt cattttactg atggtccata
     3001 cacgaatggt gctaccggcg cggcggctct atcggtagtg gaatcgggga agtacgagaa
     3061 ccgtctcgag atctatggca caaaaggcgc gttgatggtt gaagagaccg gtgagttgtg
     3121 gctctcgccg gccggatcgg gaacgtggcg gccggtacag gtggatcagg accacatggc
     3181 ccagggaatg cgcgagagta gttggtcgcg tggattcacc gccttcgcct gccggattgt
     3241 cgatgcgatg cgcagtggca agaagaccgt cgaagacgca gccagtttcg aggacggtca
     3301 tcgagtgcaa gtggtcctcg acgcggtgcg cgaatcaaat gagcgtggtt gctgggtgga
     3361 cgtcggtggg taagaggacc cgcgttggag ttgacaccgg cggcactttt acagatttcg
     3421 tctgcgagca agacggtgaa gttcatctct tcaaggttcc ctcaactcca tccgatcctt
     3481 caattgccat tcgccagggc ctcgatcgca tttgttctga aatccctgtt caactgaccg
     3541 cgctggaggt cgttcacggc acaactgtgg gcacgaacgc gttgctgcaa cgtcgaggtg
     3601 cgcgcaccgc attgatcacg accaggggct tcgaagacgt cctcgcgatt ggacggcagg
     3661 cgcggccaga actctacaac ctgaacgccg taaagccggc gccgttagtt cccgacgagc
     3721 ttcgactcgg agttgcggaa cgagttgttg cgtcaggtga agtaatcaaa ccactcgaag
     3781 acgcggaact tcccgacata gtcgacagac tgaagcgcga gaacgtcgag tccgttgctg
     3841 tttcgctact gttttctttt ctgcatccgc aacacgaaga gcaaatagca aaggtgattg
     3901 cctcgcttgg cgtgccggtt tcaatctcaa gccggatact gccggaatat cgcgagtacg
     3961 agcgcacgtc gacagttgta atcaacgctt atctacaacc gttgatgggc cgctatctag
     4021 aacgtcttac aaagcagaaa gtgcaaagct caaagctcaa ccttcgcgtg atgcagtctt
     4081 cgggtggaag catctcggca gagatggcgg cagtcgagcc tgtgcgcacg attctttctg
     4141 gaccatccgg cggcgtcgta ggcgcactgc gtgcggcaca cgctgccggc ctcaaaaaca
     4201 tcatcacgtt tgacatgggc ggcacttcaa ctgacgtcgc gctctgcgac gatggcgcga
     4261 ttcgcacgac caacgaagcg tctgtagccg gactgccggt cgcgatctcc gtgatggaca
     4321 tccacaccgt cggtgcgggc ggtggatcga ttg
//
LOCUS       DASTEY010000430         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_784003, whole genome shotgun sequence.
ACCESSION   DASTEY010000430 DASTEY010000000
VERSION     DASTEY010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784003"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFO72_07615"
     CDS             complement(<1..415)
                     /locus_tag="VFO72_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479193.1"
                     /translation="MNKNWVMSQESFDALLGWLDSDRDRAGVKYEQIRSGLIKFFTFR
                     GHCEAEDLADETINRVVSRLNEIKNQVTGDSRYFYGVARKVQMEYLRRKTPHAAPESD
                     TNPERVEIEYQCLEECISKLSDENRELVLRYYQADG"
     gene            complement(408..>2086)
                     /locus_tag="VFO72_07620"
     CDS             complement(408..>2086)
                     /locus_tag="VFO72_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET9479194.1"
                     /translation="TLSFYNLAHLERSRGNLDEAKRHIKAAIALAEDVRTKVSSQDLR
                     ATYFATVRDSYDLYIDVLMLQHKNEPAAGFDREAFAVSERARARSLLELLRDAQTNTR
                     EGVDHLPQPLTLQETQQRILNDETSLLEFALGNERSYAWLVTRNNVTSVELPSRKEID
                     QAAKRLYKAITDHQLVHGESIEARAEREAKAAAALPVEIRTVSKLLLAPLSGKLNTKR
                     LLVVADGALQYIPFQILIDPQSHEQLIKSHEIVNQPSASTLAIVLSEISSRKTAANLI
                     AVLADPVFEFTDPRVKRDAPGVLDQPAEMLAVRRALRDVGVTPDGLQIPRLFASGREA
                     DEIVALAPARTALKAVGFAASRDRIFSPHLASFRIVHIATHGIINSERPEQSGIVLSL
                     FDQQGRSQNGFIRLHDIYNLKLPADLVVLSACSTAFGKDVKGEGLISLTRGFMYAGAA
                     GVVASLWKVDDEATAELMKHFYAALLKKGMPPAAALRDAQLELAKQRRWQSPYYWAGF
                     VIQGQYDQKEKFGPSSIGNTELAVLAVFGGSLLLASILLLRRRRRATGNE"
BASE COUNT          421 a          558 c          597 g          510 t
ORIGIN      
        1 ggccgtctgc ctgatagtac cgcagaacca gttcgcgatt ttcatcactc agtttgctga
       61 tgcactcctc gaggcattgg tattcaatct cgacgcgttc gggattggtg tctgactctg
      121 gcgcagcgtg aggagttttt ctcctcaggt actccatttg gaccttgcgc gcgactccgt
      181 aaaaatatct ggagtcacca gtaacctggt tcttaatctc attcaaccgg ctgacaactc
      241 gattgatagt ttcatccgcg agatcttcag cttcgcagtg accgcgaaaa gtgaagaact
      301 taatcagccc gctgcgaatc tgctcgtatt tgacacccgc ccggtcgcga tctgagtcga
      361 gccagccgag aagggcgtcg aacgattcct gagacatgac ccagttctta ttcattacct
      421 gtcgcgcgcc gtcttcgccg gagcaaaagg atcgaggcga ggagaagtga tccgccgaag
      481 accgcgagca cggcgagctc agtgttgccg atcgaggatg ggccaaactt ttccttctga
      541 tcgtactgtc cctgaatcac gaaaccagcc caatagtatg gagactgcca gcgtctttgc
      601 tttgccagtt ccagttgcgc atctctcaaa gcggcagctg gtggcattcc tttctttaac
      661 agcgccgcgt aaaagtgttt catcagttcc gccgtcgctt cgtcgtctac cttccagagc
      721 gaagcgacta cgcccgcggc tccggcatac atgaagccgc gcgtcaggct aatcagacct
      781 tcacccttga cgtctttgcc gaacgccgtg ctgcatgcgc ttaacacgac cagatcggca
      841 ggcagtttca agttgtatat gtcatgaagg cgaataaaac cgttctgcga acgtccctgt
      901 tgatcaaaca gcgagagcac aatgcctgat tgttcaggcc gttcgctgtt gatgattccg
      961 tgcgtggcga tgtgcacgat tcggaaactc gcgaggtgtg gactgaaaat ccggtcgcgc
     1021 gaagccgcaa agccgacggc cttcaaggct gttctcgcgg gcgccagcgc cacgatttcg
     1081 tccgcttctc ggccggaagc gaatagtctc ggaatttgca gtccgtcagg tgtaactccg
     1141 acgtctcgca atgcccggcg aaccgcgagc atctcggctg gttggtccaa aacgcctggc
     1201 gcgtcgcgtt tcacacgcgg atcggtgaat tcgaaaaccg ggtcggcgag cacggcgatc
     1261 agatttgccg ccgttttgcg gctgctgatt tcagataaaa cgatagcgag cgtcgaggcg
     1321 gagggttggt taacgatttc gtgcgacttg ataagctgct cgtgtgattg cggatcgatg
     1381 agaatctgaa acggaatgta ttgcaatgcg ccatcagcaa cgacgagcaa tcgtttcgtg
     1441 ttgagtttgc cggagagtgg ggccaataag agtttgctga cggttctgat ttccacaggc
     1501 agcgccgccg ctgctttcgc ttcacgctct gcacgcgctt cgattgattc tccatggacc
     1561 aactggtgat ccgtgatggc tttgtagaga cgtttggccg cctggtcgat ttctttgcgg
     1621 ctcggcaact cgacactggt gacgttattt cgtgttacca gccacgcata acttcgttca
     1681 ttaccgagtg cgaattcgag caagctggtt tcatcgttaa ggatgcgttg ctgtgtttcc
     1741 tggagagtta acggttgagg aaggtgatcg acaccttcgc gagtgttcgt ctgtgcatcg
     1801 cgcagcaact cgagcagcga gcgggcgcgc gctctttcac tcacggcgaa agcttcgcga
     1861 tcgaatcctg ctgcgggttc gtttttgtgc tggagcatca ggacgtcgat atagagatcg
     1921 tagctgtcgc gaacggtggc gaagtaagtg gcccgcagat cctggctgga gaccttcgtg
     1981 cgcacgtctt cagcgagcgc gatcgcagcc ttgatgtgac gtttggcttc atcgaggttg
     2041 ccgcggctgc gttcgagatg ggccaggttg tagaagctga gtgtct
//
LOCUS       DASTEY010000431         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_437879, whole genome shotgun sequence.
ACCESSION   DASTEY010000431 DASTEY010000000
VERSION     DASTEY010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437879"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFO72_07625"
     CDS             <1..535
                     /locus_tag="VFO72_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9479195.1"
                     /translation="GIDIDPALWKDLKAPVYGDPRLTSEIPSKGPGEGEGIGNNRGTG
                     IGDGTGPGVGAGSEGNTGGGIGQNGCCGEGTGLGARASGFSSSEVDQRARLLSKPEPQ
                     YTEEARRHQISGTVMLRAVFSSSGEVVQIRALNTLPFGLTERAIAAARQIRFVPAMKD
                     GRPVSVYMQLEYNFNLY"
     regulatory      594..790
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            899..>2202
                     /gene="lysC"
                     /locus_tag="VFO72_07630"
     CDS             899..>2202
                     /gene="lysC"
                     /locus_tag="VFO72_07630"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HET9479196.1"
                     /translation="MPVVMKFGGTSVADAAALENVARIVAASRDNGPVVVVSALSGVT
                     DALLASTRLAVESTVDEARASLDETFRRHREAAKELLPVHAADRFIAYLNNAAAQISQ
                     LLQRTSDYPASQLATQDAIVSFGEMLSSALVAEVLNHRKVNAQQVDPRHCVITNDDYT
                     SAAPLVAETFAKGRQLLLPLIEENVVPVVGGFVGATADGTTTTLGRGGSDYTAALLGA
                     ALRVAEIQIWTDVTGVLTADPRVVPDTQTVERLSYGEAAELAYFGAKVLHPKTIQPAI
                     EDGIPVRICNSRAPQERGTLVGPQTETSPRTIKAIAHKTGVTTVQITSARMLGAYGFL
                     RALFEVFDRHRTVVDVVTTSEVSVSLSLDDDSALPAIVAELEQLGTVRVEKGLAIICV
                     VGEGMGGTPGIAARVFSTISDINVTIISQGASSINFTFAVEEP"
BASE COUNT          474 a          614 c          663 g          451 t
ORIGIN      
        1 gggcatcgac atcgatccgg cactgtggaa agacctcaaa gcacccgttt acggagatcc
       61 cagattgact tcggaaattc cctcgaaggg accaggcgaa ggcgaaggaa tcggcaataa
      121 ccgaggaacg ggcatcggcg acggtactgg accaggcgtt ggcgccggta gcgagggaaa
      181 tactggaggc gggattggac aaaacggctg ctgcggagaa ggaaccggac ttggcgccag
      241 agcgagcggg ttcagttcat ctgaagtaga tcaacgagcg cgcctcttgt cgaagccgga
      301 accgcagtac acggaggaag ctcgccgtca tcaaatcagc ggcaccgtca tgctgcgggc
      361 tgtgttttcg agctccggcg aagtggtcca gatacgcgct ctcaacacgc ttccatttgg
      421 acttacggaa cgagccattg ccgccgcgcg ccagatcaga tttgttcctg cgatgaaaga
      481 cggccgtccg gtgtcggtct acatgcagct cgaatacaat ttcaacttgt attgattcct
      541 tgacatcgcg ggtgcttccc gctaagttgt gccagcattt ttcattgatc ttttgagcga
      601 gtagaggcgc gacctccaac agtaagcaaa ccaacggcga aggagcgggt ttgcggaaag
      661 gggcaggtcg ccgaagcttg cggtcgtttc cttcaaacgg cagcgagttg gttgccgcgg
      721 ctaaatccgc gggactgtca ccgatgtcat ccgcggttcg gatgaattcc ggtggagtgc
      781 tacgacgaag aatgcgtgac gtgcggaaga ggcgcgtctc acgaattcac gattctccaa
      841 aagaggtcgg tcgtgttcac tcgcggccgg ccttttttga tcgctattga gatctcacat
      901 gcccgtcgtg atgaaatttg gcggaacgtc agtcgctgac gcagcggcgc tcgaaaacgt
      961 ggcgcgcatc gttgcggcgt ctcgtgataa tggaccagtc gtggtggtgt cggcgctgag
     1021 cggcgtgact gatgccttgc tggcgagcac gcgcctggcg gtcgaaagta ctgtcgacga
     1081 ggcgagagca tctctcgatg agacgttccg ccgtcatcgc gaagcagcca aagaactgct
     1141 gccggttcat gccgcagatc gtttcatcgc atacctcaac aacgccgcag cgcaaatctc
     1201 acagctattg caaagaacgt cggactatcc cgcgagtcaa ctcgctacgc aggacgccat
     1261 cgtttctttt ggcgagatgc tttcaagcgc cctcgtcgca gaagttctaa atcaccggaa
     1321 agtgaacgcg cagcaagttg atccacgaca ctgcgtgatc acaaacgacg attacacctc
     1381 tgctgcgccg ttggtggcgg aaacgttcgc aaagggccgg caattgctgt tgccgcttat
     1441 cgaggagaac gttgtgccgg tcgtcggcgg ttttgtcggc gcaactgctg atggaacgac
     1501 aaccacgcta ggacgcggcg gttcggatta cacggccgcg ctcctcggtg cggcgcttcg
     1561 cgtcgcggag attcagatct ggaccgatgt caccggcgtc ttaactgccg atccacgcgt
     1621 cgttcccgat acgcaaacag tagaacgact gtcttacggc gaggccgcag agcttgctta
     1681 cttcggcgcg aaggttctgc acccgaagac gattcagccg gcaatcgagg acggcattcc
     1741 ggtgcggatc tgcaactcac gcgcgccgca agaacgcgga acccttgttg gtccacaaac
     1801 cgaaacctcg ccacgcacga tcaaagcgat cgcgcacaag accggcgtca cgactgtaca
     1861 gatcacttca gcacgaatgc tcggggctta cggttttctt cgtgcacttt tcgaagtgtt
     1921 cgatcgtcat cgcacggtcg ttgacgttgt gaccacctcg gaggtcagcg tttcgctttc
     1981 gttggacgat gacagcgcgt tgcccgcgat agttgcagaa ctggagcagc tcggaaccgt
     2041 ccgcgtggaa aaggggctcg caattatttg tgtggtgggc gaaggtatgg gcggcacgcc
     2101 cggcatcgcc gcgcgcgtgt tcagcaccat cagcgatatc aacgtcacga tcatctccca
     2161 gggcgcttcg agcatcaact ttacttttgc agtcgaagag cc
//
LOCUS       DASTEY010000432         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_174407, whole genome shotgun sequence.
ACCESSION   DASTEY010000432 DASTEY010000000
VERSION     DASTEY010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174407"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VFO72_07635"
     CDS             <1..1191
                     /locus_tag="VFO72_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9479197.1"
                     /translation="AAGTIRERLQSAMKINIIGGGPAGLYFAILMKKADPSHQIRVYE
                     RNGPDDTFGWGVVFSGKTLANLRAADEESHAEITKQFEAWDNVDVVHRDTKISIHGNS
                     FSGIARLQLLKILQRRAEQLGVQMSFRTEIHDIDALRNESDLLIAADGVNSTVRLHYL
                     EQLKPQLDLRAHRYIWYGTNQLFHGLTLTFRENAAGTFAAHSYKFNKQTSTFIVECDP
                     QTWNAAGFDRMPDDETRAYLGEVFARDLNGYPLLSNNSKWIQFLLVKNENWFFENVVL
                     LGDALHTAHFSIGSGTKLAMEDAIALAESLQKTRDVREALTRFAETRRPVIQEYQSAA
                     FESMLWFENVRKYMHASPINLALSVMTRSGKVTYEELKKRDPEFIRRFDESRSNDLSP
                     SGRL"
     gene            complement(1168..2637)
                     /locus_tag="VFO72_07640"
     CDS             complement(1168..2637)
                     /locus_tag="VFO72_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET9479198.1"
                     /translation="MWKFARPIRHQIEETRWPTADEAARSLLFSPIRIGRLEARSRTW
                     VPAMVPWRATEDGFVTQQNLDWYRRFAEGRPGVLVVEATGVRDIPSGPLLRIGDDRFI
                     AGLRKLVETVKEASHGETLLFIQIIDFLAVKRRPEKSKYFQRFLEINEQHRQALAETT
                     ERRHWLIAEEAEIRSFLESAEEGVLERILAPRELEALNFGYREHVTDTHLKHIRELPQ
                     ELPPIFAAAASRAREAGFDGVELHYAHAYTMAGFLSRQNNREDGYGGSQENRVRLPLE
                     VFRAVRDRVGDDYVVGVRFLADEVIEGGSRVDDAIYYAKEFARAGFDFLSLSKGGKFE
                     DAQQPKVGQAVYPYTGQSGYECMPTVLSDETGPFGRSVPLVAAIKHAVNAAGFQTPVV
                     ATGGISTFEQAEEILRLGQADIVGMARQALADPDWFVKVRLGRGDEVRRCTYTNYCEA
                     LDQAHRPVTCKLWDRVDLDEPGIATVDDGRRRLEPPAWR"
     gene            2690..>3454
                     /locus_tag="VFO72_07645"
     CDS             2690..>3454
                     /locus_tag="VFO72_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9479199.1"
                     /translation="MSDELLKWRNEFPILDKTVYLISHSLGAMPRATYERLQDYAETW
                     ATRGVRAWAEGWWDMPVTVGDEVARIIGADRGTVVMHQNVSVCQSLVLSCLEPTPERN
                     KIVYSELNFPSVMYVYEAHARDGKLRIETVKSDDGITVPLEKMLAAIDETTLLVPFSH
                     VLFKSAFLQDARAIIKRAHEVGAMVVLDTYQSAGTVPFSVKELDADFATGGSVKWLCG
                     GPGAGYLYVRPDLQTKLEPKTTGWMAHEAPFAFDTSL"
BASE COUNT          774 a          981 c          953 g          746 t
ORIGIN      
        1 gctgcgggaa cgatcagaga acgactccaa agtgctatga agatcaatat catcggtggt
       61 gggccggcgg gattgtattt tgcgatcctg atgaagaagg ctgacccgtc gcaccagatc
      121 cgggtttatg aacggaatgg accagacgat acgtttggtt ggggcgtggt gttttccggg
      181 aagacgctcg caaacctgcg cgctgctgat gaagagtcgc atgcggagat tacgaaacag
      241 tttgaagcgt gggacaacgt cgacgtcgtc catcgagata caaagatctc gattcacggc
      301 aacagcttct ccggcatcgc gcgcctgcaa ctgctgaaga ttctccaacg ccgggccgag
      361 cagttgggcg tccagatgag tttccgcacg gaaattcatg acatcgacgc gctgcgaaat
      421 gagtccgacc tgctgatagc cgcggatggc gtgaacagca ctgttcgctt gcattacctc
      481 gaacaactca agcctcaact tgacctgcgc gcacaccgct acatctggta cggcacgaat
      541 cagttatttc atggcttgac gctcacgttt cgcgagaatg cagccggcac atttgcggcg
      601 cattcctaca agttcaacaa gcagaccagc acgttcattg tcgagtgtga tccgcagacg
      661 tggaacgccg ccggattcga tcgcatgcct gatgacgaga cccgcgcata tctgggagag
      721 gtttttgcca gggacctgaa cggatatcca ttgctgtcga acaactcaaa gtggattcaa
      781 ttcttactcg tcaaaaacga gaactggttt tttgagaacg tcgtgctgct cggtgacgca
      841 ctgcacacag cacacttctc gatcggttcg ggtacgaagc tggcgatgga ggacgcgatc
      901 gcactggctg agtcgttgca aaaaacgcgg gacgtgcggg aggcgttgac acgttttgca
      961 gagacgcggc gaccggtgat tcaggagtat caatcggcgg ctttcgaaag catgctgtgg
     1021 tttgagaacg tgcggaagta catgcatgcg agcccgataa atttggcgct tagcgtaatg
     1081 acgcggagcg gaaaagtgac ttacgaagag ctaaagaaac gcgatccgga gtttatccgc
     1141 aggtttgacg aatcgaggtc taacgactta tctccaagcg ggcggctcta atcgccgccg
     1201 gccatcgtca acagttgcga tcccgggctc atccaaatca acgcgatccc aaagtttgca
     1261 cgtcactggc cgatgagcct gatcgagcgc ctcgcaataa tttgtataag tgcagcgccg
     1321 tacctcatcg ccacgcccaa gtcttacctt gacgaaccag tcgggatcgg cgagcgcctg
     1381 ccgcgccatg ccgacgatgt ccgcctggcc caaacgcaat atctcttccg cttgttcgaa
     1441 tgttgagata cctccggtcg ccacgaccgg cgtctgaaaa cctgcggcat tcaccgcgtg
     1501 cttgatagca gcgactagtg gaacacttcg cccgaatgga ccagtttcat cggacaacac
     1561 agtcggcatg cactcatatc cgctttgacc ggtgtatggg taaacggcct ggcccacctt
     1621 tggctgctgt gcgtcctcaa atttcccacc cttcgacaag gacagaaaat caaatcccgc
     1681 gcgcgcgaat tccttcgcat agtagatcgc atcatcgacg cgactgccac cctcgattac
     1741 ttcgtcagcc agaaagcgca cgccgacgac gtaatcgtca ccgacacgat ccctgacagc
     1801 acgaaacacc tcgagcggca agcgtacgcg attctcctgt gaaccgccgt aaccatcttc
     1861 acgattgttc tgccggctaa gaaaaccggc catcgtgtag gcgtgcgcat agtgaagctc
     1921 aacgccgtca aatcccgctt cgcgcgcgcg gctcgccgcg gcggcgaaaa tcgggggcag
     1981 ttcctgcggc agttctctga tgtgttttag atgtgtatcc gtaacgtgct cacggtagcc
     2041 aaaattgagt gcttcaagtt ctctcggcgc gaggatcctt tccagaactc cctcttctgc
     2101 tgactccaga aaactacgga tttccgcttc ttcggcgatc agccagtgac ggcgttctgt
     2161 agtctcagcc aatgcctggc ggtgctgctc attgatctcc aagaaccgtt gaaagtactt
     2221 gctcttttcc ggacgccgct tcaccgcaag gaagtctatg atctgaatga acagcagagt
     2281 ttcgccatga ctcgcttcct tcaccgtctc tacaagcttc cgcagccctg ctataaatcg
     2341 atcatcgccg atgcgcagta atggaccact gggaatgtcg cgcacgcccg tcgcctcgac
     2401 aacaagcacg cccggacgtc cttccgcgaa gcggcggtac cagtcgagat tttgctgagt
     2461 aacaaacccg tcctctgtcg cacgccatgg caccatcgcc ggcacccacg tgcgactacg
     2521 cgcctccagc cgtccaatgc gaatcggact aaacagcagc gaacgggctg cttcgtcagc
     2581 tgttggccag cgagtttctt ctatctggtg tctgattggc cgcgcgaact tccacatctt
     2641 agccacagat tacacgattc tggtacattt accgcaggag ggttctttct tgtctgacga
     2701 gttactaaag tggcgcaacg agtttccgat tctcgataag actgtctact tgatctcgca
     2761 cagtctgggc gcgatgccca gagccacgta tgaacggctt caggattacg ctgagacgtg
     2821 ggccactcgc ggagtgcgcg cgtgggcgga aggctggtgg gacatgccgg tgacggtcgg
     2881 cgacgaagtc gcgcgcatca ttggcgccga ccggggcaca gtcgtgatgc accagaacgt
     2941 ttctgtctgc caatcactgg tgctgtcgtg tctggaaccg acaccggaac gaaacaagat
     3001 cgtctactcg gaactcaatt tcccttcggt gatgtacgtc tacgaggccc acgctcgaga
     3061 cggcaagctt cgcatcgaga cggtgaaatc ggacgacggt ataaccgtac cgttggaaaa
     3121 gatgctggcc gcgatcgacg aaacaacatt gctcgtgccg ttctcgcacg tactttttaa
     3181 gagcgcattt ttgcaggacg cgcgcgcgat catcaaacgc gcgcatgaag tcggtgcgat
     3241 ggtagttttg gatacgtacc aatctgcggg cacggtgccg tttagtgtga aggaactgga
     3301 cgcagacttc gcgacgggcg gatccgtgaa atggctttgt ggcggaccag gcgccggcta
     3361 tctgtacgtg cggccggatc tgcaaaccaa acttgaaccg aagaccaccg gttggatggc
     3421 ccacgaggcg ccgtttgctt tcgatacttc actt
//
LOCUS       DASTEY010000433         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1492955, whole genome shotgun sequence.
ACCESSION   DASTEY010000433 DASTEY010000000
VERSION     DASTEY010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492955"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /gene="secY"
                     /locus_tag="VFO72_07650"
     CDS             complement(<1..1249)
                     /gene="secY"
                     /locus_tag="VFO72_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9479200.1"
                     /translation="MEKFLAAVRNMFNVPDLRRRIFFTLGLLAVYRFGAHVGAPGINK
                     ARLDEVWRDVAGTLLGVLDLFSGGNFRTISVFALGVTPYITASIILQLMTVVSPQLKK
                     LQEEGEMGRQKINQWTRYLTVGLAAIQTWFVAHWLQVNGVGAPTWGFLFTTILTLTTG
                     TIFVMWLGEQITERGVGNGISLLIFAGIVIGLPNGAQQVLGKVRSGDTMEVVGVIALI
                     IALIAIIAFIVFVESARRKIPVSYAKRHVGRQLVGGQQTTMPLKINMGGVIPVIFASS
                     VLSMPQSLFAAFPPDPADVNTTTWGKIWTFFQTFHASDPYYELIFLSLIIFFTFFYVS
                     IVFNVEEVADNLRKHGGFMPGIRPGRATAEHLNSILTRLTTVGAIYLALVAFVPQFML
                     SGFKVARLPLIGGWLDAFISTTPG"
     gene            complement(1279..1728)
                     /gene="rplO"
                     /locus_tag="VFO72_07655"
     CDS             complement(1279..1728)
                     /gene="rplO"
                     /locus_tag="VFO72_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET9479201.1"
                     /translation="MGLNTLKPAKGSTHKKKRVGRGPGSGLGKTAGRGEKGQKSRSGY
                     SRKIGFEGGQMPLHRRLPKRGFTNIFKKRWLEITLAALDQHFNADEEITPEVLHTRGL
                     IKKAKHDVVVLGNGEVSKPLRVSAHRFTKSAREKIEKAGGAVVELAG"
     gene            complement(1761..1967)
                     /gene="rpmD"
                     /locus_tag="VFO72_07660"
     CDS             complement(1761..1967)
                     /gene="rpmD"
                     /locus_tag="VFO72_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET9479202.1"
                     /translation="MPRRAETQSENGKIRIQYYRSFIASPEKHKSIVRSLGLRKLNQI
                     VERPDTEAMRGAVAKVPHLLRIVE"
BASE COUNT          467 a          608 c          531 g          443 t
ORIGIN      
        1 aaccgggcgt agtgctgata aacgcatcca gccacccgcc gatcaacgga agccttgcta
       61 ctttgaatcc actcaacatg aactgtggca cgaacgcgac cagggccaga tagatcgcgc
      121 ccactgtcgt gagcctggtg agaatcgaat tcagatgttc cgccgtggct cgaccaggac
      181 gaatacccgg catgaagcca ccgtgcttgc gcaggttgtc agcgacctct tccacgttga
      241 atacgatcga cacgtaaaag aacgtgaaga agataattag cgagagaaag atcaactcgt
      301 agtaaggatc gctcgcgtgg aaagtctgaa agaacgtcca gatcttaccc cacgtagtcg
      361 tattcacatc ggcagggtct ggcgggaagg ccgcaaacaa gctctgcggc atcgacaata
      421 ccgacgaagc aaagatcacg ggaatcacgc cgcccatgtt gatcttcagc ggcatcgtcg
      481 tttgttggcc cccgacgagt tgccggccga cgtgccgctt tgcataactg accggtatct
      541 tcctgcgcgc tgactcgaca aacacaatga acgcgatgat ggcgatcaac gcgatgatca
      601 atgcgataac gccgacgact tccatcgtgt cgccgctacg aactttgcca agcacttgct
      661 gtgcgccatt cggcagacca atgacgatac ccgcaaagat cagcaacgag attccgttac
      721 ccacaccgcg ttcggtgatc tgctcgccca accacatgac gaagatcgtt ccggtggtga
      781 gcgtcaaaat tgtggtgaac agaaaacccc aggtgggtgc gccgacgcca ttgacctgta
      841 gccagtgggc cacaaaccac gtctgaatcg cggcaagtcc gacggtcaga tagcgggtcc
      901 actgattgat cttctgccgg cccatctcgc cttcttcctg caacttcttg agttgcggtg
      961 aaacgacggt catgagctgc aagataatcg acgcggtgat ataaggtgtt acaccgagtg
     1021 cgaatacgga gatggtgcgg aagtttccgc cggagaaaag gtcgagtacg cccagcagcg
     1081 tgccggcaac atcgcgccag acttcatcga gccgtgcttt gttgataccc ggtgcgccga
     1141 cgtgggcgcc gaagcggtag accgcgagca ggcccagcgt aaagaaaatg cgccggcgta
     1201 gatccggcac attgaacata ttgcgaacgg cggctaaaaa cttttccatc acaaaccctc
     1261 cgggtagtga gcgccgggtt atcccgcgag ttccactacc gcaccaccag ccttctcgat
     1321 cttttcgcga gcactctttg tgaaccggtg cgctgaaaca cgaagcggct tggaaacttc
     1381 accgtttccg agcactacca catcatgttt cgcctttttt atcaagccgc gcgtgtgcag
     1441 cacttccggc gtgatctctt cgtcggcatt gaaatgctga tcgagcgcgg cgagagtaat
     1501 ttcgagccag cgcttcttga agatgttggt gaaaccgcgc tttggcaagc ggcgatgtag
     1561 cggcatctgg ccgccttcaa acccaatctt gcgcgagtag cccgatcgag acttctgtcc
     1621 cttctcaccg cggcccgcgg ttttgcccaa accggatccc ggcccgcgac cgacgcgttt
     1681 ctttttgtgg gtcgatccct tggcgggttt aagcgtatta agaccaattg ccatcatcca
     1741 ctccactatc catgaaatcc ctattccact atgcgtaaca agtgcggcac cttcgctacc
     1801 gcgccacgca tcgcctcagt gtccggccgc tcaacgatct gattgagttt acgcaaaccc
     1861 aaactgcgca cgatactctt gtgtttctcc ggcgaagcaa taaaactccg gtagtactga
     1921 atccggatct ttccgttttc actttgcgtt tctgctctcc gcggcataac ctctccgttc
     1981 aaactaactt tcctttacct gggaccgcag gcgtctcgcc tgcgggatac gcacttagtc
     2041 tgttgcgct
//
LOCUS       DASTEY010000434         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_569988, whole genome shotgun sequence.
ACCESSION   DASTEY010000434 DASTEY010000000
VERSION     DASTEY010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569988"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VFO72_07665"
     CDS             <1..1448
                     /locus_tag="VFO72_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9479203.1"
                     /translation="VFVNGEPRFEQQLKSGDRITIGASTLRFEQPVEERTTDVRYDDK
                     PLGHTQLLISANDVMSTVLRSKEDVPASAQRDKVLEALQRKANILSALYEMSKTLGSV
                     FDLNAIFEKATDIIFRSTPADRVVALLEEPGEGTGGDVNLTPVAVRARDNKLEAHARR
                     LSIGRTITRKVMRERVALLSQDAASDEQFAGVDSIVSQGVRSTICAPLMGESRVHGAL
                     YADRLDPFAAFKPDDLELISAVAAQTAIAVENARAHERLAREEVARANYSRFLPEYVV
                     QQMLLNPDSFKLGGVSQTITILFADIRGFTRISEHAPPEKIVGLLNHYFSAMTDIIFA
                     HGGTLDKYLGDGLMALFGAPTTTPDDASNALNAAVAMQRRLLGINRELREEGFPEIGV
                     GMGLHTGEVIVGYIGSERRSEYTAIGDTVNTSSRLESNAKGGEILISDATAKAARSRY
                     KLKPREAIMVKNRQQPVMLWEVDWQKASGSF"
     gene            1482..>2099
                     /locus_tag="VFO72_07670"
     CDS             1482..>2099
                     /locus_tag="VFO72_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9479204.1"
                     /translation="MSFIGKTGHFLGLSALRKFVSDSMAIDMGSASTLIAVRGRGVVV
                     DEPSVVAVNKLTGEVIAFGNEAHKMQGREARDVTVIAPLVDGVVADFERTRLMLDHFV
                     REARSGVSHFSRRAIMSVISEVTQVEQRALLAAAEEARISRVYMIEEGQAAAIGAGVP
                     LSDEHAAAVVDIGGGTTNVAAIISGSIIASRAERIGSGDIDLAIMD"
BASE COUNT          461 a          609 c          615 g          414 t
ORIGIN      
        1 aagtcttcgt caacggtgag ccacggttcg agcagcaact caagagtggc gaccgcatca
       61 ccatcggcgc gagcacgttg cgttttgaac aaccggtcga agaacgcacc actgatgttc
      121 gttacgacga taaaccactc gggcacactc aactcttaat ctcggcgaac gacgtcatgt
      181 cgacggtgct gcggtccaaa gaagacgtgc ctgcttcggc gcaacgcgac aaagttctcg
      241 aagcacttca acgcaaggca aacatcctga gcgcgcttta cgaaatgagt aagacgctcg
      301 gctcggtgtt cgatctgaac gcgatcttcg agaaggccac cgacatcatc tttcgctcga
      361 cgcctgccga tcgcgtggtt gcattgctgg aagaacccgg cgaaggaacc ggcggcgacg
      421 tcaacttgac gcccgtcgct gtgcgcgcac gtgacaacaa gctcgaagca catgcgcgcc
      481 ggctttcgat cggccgcacc atcacgcgga aagtgatgag ggaacgcgtt gcgctgctct
      541 cgcaggacgc agcctcggac gaacagtttg ccggcgtgga ttcgatcgtt tcgcaaggtg
      601 tgcgttcaac catctgcgcg ccactgatgg gcgagtcgcg agttcatggc gcactctacg
      661 ccgatcgtct tgatccattt gctgctttca agcctgacga tctcgaactg atcagcgccg
      721 tggcggccca gacggcaatc gccgtcgaaa acgcccgcgc acacgaacgg cttgcgcgcg
      781 aagaagttgc ccgggccaac tacagccggt ttttgcccga atacgtcgtc cagcagatgc
      841 ttttgaaccc cgactcattc aagctcggcg gcgtcagtca gaccatcaca atcctttttg
      901 ccgacatccg cggtttcaca cggatctccg aacacgcgcc gcctgaaaaa atcgtcggcc
      961 tgctgaacca ttacttctca gcaatgactg acataatctt cgcccacggc ggaacacttg
     1021 ataaatatct cggcgatggt ctaatggccc tatttggtgc gccaacaacg acgcccgatg
     1081 atgcatccaa tgccttgaac gccgccgtgg ccatgcagcg ccggctcctc ggcatcaatc
     1141 gggaattgcg cgaagagggt tttcccgaga tcggcgtcgg catgggtctt catacgggcg
     1201 aagtgatcgt cgggtatatc ggctccgagc gtcgttcgga gtacacggcg attggcgata
     1261 ccgtgaacac ctcgtcacgg ctcgaatcaa acgctaaggg cggagagatt ctgatcagcg
     1321 atgcgacggc caaggccgcg cgtagccgtt ataaactaaa gccgcgcgaa gcgatcatgg
     1381 tgaagaacag gcaacaacct gtgatgctgt gggaggtcga ttggcaaaaa gcaagcggtt
     1441 cgttctgatt ggtaggtcct cgagtcagga cctataagtt tatgtcattc ataggtaaga
     1501 ccgggcattt ccttggactt agcgcgctgc gcaagttcgt ttcagactcc atggccatcg
     1561 atatgggctc cgcgagcacc ctcatagccg tgcgtggccg cggtgtggtc gtcgatgaac
     1621 catcggttgt ggccgtcaac aagctcaccg gcgaggtcat cgctttcggc aacgaagctc
     1681 acaaaatgca aggccgcgaa gcgcgtgacg tgactgtcat cgcgccgctc gtggacggcg
     1741 tggtggctga ctttgaacga accagattga tgctcgatca cttcgttcgt gaagcacgaa
     1801 gcggtgtgtc gcatttcagc cggcgcgcga tcatgagcgt gatctctgaa gtgacgcagg
     1861 tcgaacaacg cgcactgttg gctgccgcgg aagaggcgcg catcagtcgc gtctacatga
     1921 tcgaagaagg tcaggcggct gcaatcggcg cgggcgtgcc actcagtgac gaacacgccg
     1981 cagcggtcgt cgatatcggc ggcggcacga ccaacgttgc cgcaatcatc agcgggtcga
     2041 tcatcgcttc gcgtgcggaa cgaatcggca gcggagacat cgatctggcg atcatggac
//
LOCUS       DASTEY010000435         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_602428, whole genome shotgun sequence.
ACCESSION   DASTEY010000435 DASTEY010000000
VERSION     DASTEY010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602428"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFO72_07675"
     CDS             complement(<1..254)
                     /locus_tag="VFO72_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET9479205.1"
                     /translation="MSEKKLWGGRFTGGADPQFAEFNNSFRFDRRLFEADVTASIAYC
                     RALEKAGVITADEGTQIRNALDTILKSGAADNDAVEDVHS"
     gene            317..736
                     /locus_tag="VFO72_07680"
     CDS             317..736
                     /locus_tag="VFO72_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479206.1"
                     /translation="MNKHARQQKILSLIQAKPIGTQEDLRALLERAGVPATQSSVSRD
                     LEALGIVKQHGHYTLPRANGSSARGLLSLDQAGDSLVIARTLPGLASAVAVEIDAAAL
                     PEVVGTLAGEDTIFIAVRDAKAQRLTIKRVWELFSHE"
     gene            742..1938
                     /locus_tag="VFO72_07685"
     CDS             742..1938
                     /locus_tag="VFO72_07685"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET9479207.1"
                     /translation="MNTNVKKVVLAYSGGLDTSVMLRWLKEHYHCEVICFCADVGQGA
                     ELSGLEEKANATGASKLYVEDLREEFVRDYVWTAVKANAVYEGVYLMGTSLARPVIAK
                     RQIEIARKEGAVAVAHGATGKGNDQVRFELTYYALQPAIKVIAPWREWEFKGRADLIA
                     YAKQHDIPVTATVEKPYSTDANLMHISYEGGILEDPWAEPPESIFQLTRSPENARNEA
                     QYIEISFDRGEPVAIDGKKHGPVALLETLNKLGGEHGIGRVDLVENRFVGMKSRGVYE
                     TPGVTILHAAHRALESLTLDREVMHLRDSLSVKFAESIYYGFWFAPEFKLLRKTIEET
                     QTKVTGDVRLKLFRGNAIVVGRRSPHSLYDERVATFEADTVYNQRDAEGFIKLNALRL
                     RLGGDR"
     gene            1935..3002
                     /locus_tag="VFO72_07690"
     CDS             1935..3002
                     /locus_tag="VFO72_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479208.1"
                     /translation="MTEINVIDLRVIDVDKIGSATSPLALAKTVALVSSTESPKAGDV
                     VVVRALTDSATYNLLELPTGRLAKINPGDLLLGVLGRRRALKGFVGDVPQIVRNGDKL
                     HLLNMGGVIGYCTGHHSSLSDAIELEVIGLACDEEGTVLNIGDACLTPRTRLGETAPI
                     VMIAGTCMNSGKTVAATELIKQATRAGLKVAAGKLSGVACLRDTLNMSDHGAIATASF
                     LDCGLPSTVDVEDLAPTAKAIIAKLNEPSPDLIVIELGDGILGGYSVDTIFGDLELLE
                     STAALVFCASDYVGAWGGIELLRRRGVEVAVISGSVTDSRMGEDYIKEQFSVPAANAR
                     REGAALFNLVMEKVQSVSSLV"
     gene            3007..4041
                     /gene="argC"
                     /locus_tag="VFO72_07695"
     CDS             3007..4041
                     /gene="argC"
                     /locus_tag="VFO72_07695"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9479209.1"
                     /translation="MQLAQHKIRVAIFGGSGYGGSELLRILLFHPNVELVFVTAKEHA
                     GKPVGEVHRNLHGLSELSFVKDPETLDGVDCAFFALPHGQAMSIVPGLPSSIKAVDLS
                     GDFRLRDESLFEKHYKQQHTAMTAQRDFVYGLTETKREAIRRARLIANPGCFATATLL
                     GLVPLVANGLLNGRVVVDAKTGSSGSGAKAAANTHHPQRMNSFYAYKPFTHRHVPEIE
                     QELNHAGDWTNELVFMTHSLPVSRGIFASIYAETKRELSETDLRQLYADYYHDSFFVR
                     LVNGSPDINWVKTTNFCDIGFAVRAKQVVVFSAIDNLVKGAAGQAVQNMNLMFGLDEK
                     TGLILVGTNP"
BASE COUNT          905 a         1122 c         1213 g          888 t
ORIGIN      
        1 aatgaatgca cgtcttccac tgcgtcattg tcagcggcgc cgcttttgag gattgtgtcg
       61 agagcgttgc ggatctgcgt tccttcatct gccgtgatga cgcccgcctt ctccaacgcc
      121 cggcaataag caatactggc ggtgacgtcc gcttcgaaca gtctgcgatc gaagcgaaag
      181 gaattgttga attccgcaaa ctgcggatcg gcgcctcctg tgaaccggcc accccaaagt
      241 tttttctcac tcattgtttg acccagcaca actgccgtgc tagtttatgc atctatgcac
      301 cacttatgca acatttatga ataaacatgc gcgacaacag aagattttaa gcctgattca
      361 ggccaagccg attggcacgc aggaagactt gcgcgccctg ctcgaacggg ccggcgtgcc
      421 ggcgacgcag tcgagcgtgt cgcgcgattt ggaagcgctc ggcatcgtca agcagcacgg
      481 ccattacacg ttgccgcgcg caaatggctc gtccgcgcgc ggactgctga gtttagacca
      541 ggcgggcgac agtttggtaa tcgcgcgcac gctgccggga cttgcttccg cagtcgcagt
      601 cgaaattgac gcagcggcat tgccggaagt ggtcgggacc cttgcgggtg aagacaccat
      661 ttttattgca gtgcgcgatg cgaaagcgca acggctaact attaagcggg tttgggaact
      721 ctttagtcac gaatgacaca gatgaacacg aacgtcaaaa aagtcgtcct tgcctattcg
      781 ggcggtctcg acacgtcagt aatgcttcgc tggctgaaag agcattacca ctgcgaagtt
      841 atttgcttct gcgccgatgt gggccagggc gccgagctgt ccggtctcga agaaaaggcg
      901 aacgcgacgg gcgcgtcgaa actctatgtc gaggatctgc gcgaagagtt tgtgcgtgac
      961 tacgtctgga ccgcagtcaa ggcaaacgcg gtttacgaag gcgtctatct gatgggcacg
     1021 tctctcgcac gccctgtgat cgccaaacga cagatcgaaa tcgcgcgtaa ggaaggcgcc
     1081 gtcgcggtgg cacacggagc aacgggcaaa ggtaacgatc aggttcgctt tgagctaacg
     1141 tattacgcgc tgcaaccggc catcaaagtc atcgcgccgt ggcgcgaatg ggaattcaaa
     1201 ggccgcgcgg atctgatcgc atacgcgaaa cagcacgaca ttcctgtcac cgcgacggtc
     1261 gagaaaccgt attcgaccga cgccaacctg atgcacatct cttacgaagg cggaattctc
     1321 gaagacccct gggccgagcc acctgagagc atctttcaac tcacgcgctc gccggagaac
     1381 gcgcggaacg aagctcagta catcgagatc tctttcgacc ggggcgagcc cgtggccatc
     1441 gatggaaaga aacacggtcc ggtggcacta ctcgagacgc tcaacaaact cggcggcgaa
     1501 cacggcatcg gccgcgtcga tttggtcgag aaccgtttcg taggcatgaa gtcacgcggc
     1561 gtgtacgaaa cgcccggcgt gacgatcctg cacgctgcgc atcgcgccct tgagtcgctg
     1621 acacttgata gagaggtaat gcacctgcgc gattcgctct ctgtgaagtt tgccgagagt
     1681 atctactacg gcttctggtt tgcgccggag ttcaaactgt tacgcaagac aatcgaagag
     1741 acgcagacga aagtcacggg cgacgttcgt ctgaaactgt ttcgcggcaa tgcaatcgtt
     1801 gttggacgcc gttcaccaca ttcgctttac gacgagcgtg ttgcgacctt tgaggccgac
     1861 accgtctaca accagcgcga tgctgaaggc tttatcaaac tgaacgcgct gcggctgcgg
     1921 ttgggaggtg acagatgact gaaatcaacg ttatcgacct cagagttatc gatgtcgaca
     1981 agattggatc cgctacatca ccccttgcat tggccaagac cgtagcgctt gtttcatcca
     2041 ctgagtcacc gaaagccgga gacgtggtcg tggtacgcgc gctgaccgac agcgcgacct
     2101 acaacttgct cgagttgccg accggacgtt tggcgaagat caatcccggc gatctgttgc
     2161 ttggcgtgct tggccgccgc cgcgccttga aaggttttgt cggagatgtg ccgcagattg
     2221 tgcgaaacgg cgacaagtta catctgctca acatgggcgg cgtgattggc tactgcaccg
     2281 gacatcattc gagcttgagt gatgcgattg agttggaagt aatcgggttg gcgtgcgacg
     2341 aagagggcac ggtgctgaac attggcgacg cgtgccttac gccgcgaaca cgtctgggcg
     2401 agacggcgcc gatcgttatg attgccggca cgtgcatgaa cagcggcaag acggttgccg
     2461 cgacggaatt gatcaaacag gcgacgcgtg ctggtttgaa agtggctgcc ggaaagctgt
     2521 cgggtgttgc ctgtctgcgc gacacgttga acatgtcgga ccacggtgcg attgcgaccg
     2581 cgagttttct cgactgcggt ttgccttcga cagtcgatgt tgaagatctc gcgccgacag
     2641 caaaggcgat cattgcgaag ctaaacgaac cgagcccgga tctgatcgtg atcgaattgg
     2701 gtgatgggat tttaggcgga tactccgtcg atactatctt cggggatttg gaactgctcg
     2761 agtctacggc ggcgcttgtg ttctgtgcgt ctgactacgt tggcgcctgg ggcgggattg
     2821 aattgctgcg ccggcgcggc gtcgaggttg ctgtgatctc cggttcggtg accgactccc
     2881 ggatgggtga agactacatc aaggagcagt tctccgtgcc tgcggcgaat gcgcgacgtg
     2941 aaggcgcagc gctgttcaat ctggtgatgg agaaggttca atccgtgtcg tccttggttt
     3001 aagaccatgc agttagcaca acataaaatt agagtggcga tcttcggcgg ctctggttac
     3061 ggcggctcgg agctgctccg tattctgctc tttcatccga acgtcgaact ggtattcgtc
     3121 acggccaagg agcatgcggg caaacccgtt ggcgaagtgc accggaacct ccacggcctg
     3181 agtgaactga gcttcgtaaa agatcccgaa acactcgatg gtgtcgactg cgcatttttc
     3241 gcgctgccgc acggccaggc gatgtcgatt gttccgggtc taccgtcgtc aatcaaagca
     3301 gttgatctat cgggcgactt ccgtttgcgg gacgaatccc tgttcgaaaa acactacaaa
     3361 caacagcaca ctgcgatgac tgcgcaacga gactttgtct acggcttgac tgaaacaaaa
     3421 cgcgaagcaa tacgtcgggc acgcttgatt gccaatcccg gttgttttgc gaccgcgacg
     3481 ttgcttggtc tcgtgccgct tgtcgccaac ggcttgttga acggccgcgt cgttgtcgat
     3541 gcgaagaccg gatcgtccgg ctccggcgcg aaggctgccg ccaacacgca tcacccgcag
     3601 cgaatgaatt cgttctacgc gtacaagccg ttcacgcatc gacacgtgcc ggagatcgaa
     3661 caggagctca atcacgccgg cgactggacc aatgagctgg tgtttatgac tcactcgctt
     3721 cccgtttcgc gcggcatctt cgcgtctatc tacgcggaaa ctaaacgtga gttgagtgaa
     3781 acggacctgc gtcaacttta cgctgactac tatcacgact ccttcttcgt ccggctcgtg
     3841 aacggttcgc cggacatcaa ctgggtgaag acaacaaact tctgtgacat cggtttcgct
     3901 gtgcgcgcca aacaagtggt cgtcttttcc gccatcgata accttgtgaa gggcgcggcc
     3961 ggacaagcag tgcaaaacat gaatctgatg tttggtctcg atgagaagac cggactgata
     4021 ctagtgggaa caaacccgtg aaatccgtgg aatccgtggt tcaacacgag gcgcagtttc
     4081 aactctcgac ctacaagaaa atgtgggttg tcgctgaacg cggcgagg
//
LOCUS       DASTEY010000436         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1608103, whole genome shotgun sequence.
ACCESSION   DASTEY010000436 DASTEY010000000
VERSION     DASTEY010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608103"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFO72_07700"
     CDS             <1..415
                     /locus_tag="VFO72_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479210.1"
                     /translation="AAAALLRLAILKNNERYRELAQTIFGEMADSARRYPSGFGYALS
                     AMDFLLSSPKEIAILGKDQADIKPLLQEVWRKYVPNKVVALGFVDDAEAIDALPLLQN
                     RPLIDGRATAYVCQNYTCKQPVTQPQDLLDQLTSV"
     gene            533..>2003
                     /locus_tag="VFO72_07705"
     CDS             533..>2003
                     /locus_tag="VFO72_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9479211.1"
                     /translation="MNSTCTVLLAAVSVVSLVLLLLNEGLGLGLLAVSISVIVCLFYR
                     SNQKRLQELNAAEAERRHHAEEMATIHMNTIESLAIAIDAKDQTTHGHVRRTQIYATQ
                     MGKLLNVSETDLRALHAGALLHDIGKLAVPEYILNKPGKLTEAEFAKMKIHPTVGGDI
                     LKRVNFPYPVEDIVRYHHEKWDGSGYPKGLKGEQIPLIARIISVVDFYDATRCDRPYR
                     KGMKREDSLALLNSMVGTAFDPRVVRKFVEHVEEFDRLIDSEDIQEQVSSTPALDTQT
                     STKPDAGLASDILGTPAEDSGFRSISEAQREVFALHEIAQTIGSSLNLSDTVTLVANK
                     LKSIVPFDSCVIFLVDERSGKAVAAHAVGEEAELFSSRRITVGDGITGWVIANSRSMC
                     NASPELDMVGISEQIVKRFRGVLVSPLLREDGAFGAIALYSQSRTSYSSEHVRLLESV
                     CQHASSALNNALTYEKTRESALVDPLTELPNARGFYMMLE"
BASE COUNT          453 a          584 c          539 g          427 t
ORIGIN      
        1 ggccgcggct gcgctattgc ggctggccat tctcaaaaac aacgaacgct accgcgaact
       61 tgcgcagact atcttcggcg aaatggccga ctccgctcgg cgctacccgt ccggtttcgg
      121 ttatgcgctc tcggcgatgg atttcctgct gtcgagtcca aaggagattg cgatcctcgg
      181 caaagaccag gccgacatta aacctttgct ccaggaagtc tggcgcaaat acgtgccaaa
      241 caaagtagtt gcgctcggct tcgtcgacga cgcagaagcg atcgacgccc tcccgttact
      301 ccagaaccgg cctttgattg acggccgcgc cactgcttac gtttgccaga actacacctg
      361 caaacagccc gtcacccagc cgcaggactt acttgaccaa ctcacatccg tgtaatccgt
      421 ggcgaaacct tgactgaaac acactgaaac gcctatagtt atgcacgtgc caacattgtt
      481 tggcacgtcc caaacctaag cttcagctcc ttgcctgggc cccatgctga aaatgaactc
      541 cacgtgtacg gttctacttg ccgcagtttc tgtggtcagt ctcgtattgc tcctgctcaa
      601 cgaaggcctc ggcctcggcc tgcttgcggt gtcgatctcc gtcatcgttt gcctgttcta
      661 ccgctccaac caaaaacgac ttcaggagtt gaatgcggcc gaagctgagc gccgccacca
      721 cgccgaggag atggcaacga ttcacatgaa cacgatcgaa tcgctcgcca tcgcgatcga
      781 cgcgaaggac caaacgactc acgggcacgt gcgccgtacc caaatctacg cgacgcagat
      841 gggcaagctc cttaacgtgt cggaaacgga tctacgcgcg ctgcacgccg gagcgctgtt
      901 gcatgacatc ggcaaactcg ccgtgcctga atacatactc aacaaaccag gcaaactcac
      961 cgaagcggaa ttcgccaaaa tgaaaattca tccgacggtg ggtggcgaca ttctcaagcg
     1021 cgtcaacttc ccttacccag tcgaggacat cgtgcgctat caccacgaga agtgggacgg
     1081 cagcggttat ccgaaaggtc tcaaaggcga acagattccg ctgattgcgc gaatcatttc
     1141 agtcgtggac ttctacgatg cgacgcgctg cgatcgacct tatcgcaaag gtatgaaacg
     1201 cgaagattcg ctggcgttgc tgaacagcat ggtcggcacg gccttcgatc ccagggtggt
     1261 ccggaagttt gtcgagcacg tcgaagagtt tgatcgcctg atcgactcgg aagacatcca
     1321 ggagcaggtc tcttccacac ccgctctcga cacacagacg agcacgaaac ccgacgcggg
     1381 tttagcttct gacattctcg gcacgccggc tgaagacagc ggttttcgtt ctatctcgga
     1441 agcgcaacgc gaggtattcg cgcttcatga aatagcgcaa acgatcggtt catcactgaa
     1501 tttgagcgac actgtgacgt tggtcgcgaa taaattgaaa tcgatcgttc cgttcgatag
     1561 ctgtgtgatc tttctggttg acgaacgatc gggcaaggct gttgcagcgc acgcggtcgg
     1621 cgaggaagcg gagttgtttt ccagccgccg gatcactgtg ggcgatggca tcacgggatg
     1681 ggtgatcgcc aatagccgct cgatgtgcaa cgcttctccg gaattggaca tggttggtat
     1741 ttcggaacag atcgtgaagc gtttccgcgg tgtactggtt tcgccactac ttcgcgaaga
     1801 cggcgctttt ggcgcaatcg cgctgtattc acagagccgc acgtcgtact cgagtgaaca
     1861 cgtacgcctg ctcgagtccg tttgccagca cgcctcgagc gcgctcaaca atgcgttgac
     1921 ttacgaaaag acgcgcgaga gcgcgctggt tgatccttta acggaattgc cgaatgcacg
     1981 tggcttctac atgatgctcg agc
//
LOCUS       DASTEY010000437         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1591483, whole genome shotgun sequence.
ACCESSION   DASTEY010000437 DASTEY010000000
VERSION     DASTEY010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591483"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFO72_07710"
     CDS             <1..998
                     /locus_tag="VFO72_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET9479212.1"
                     /translation="QLTQALNRLGIPLPDSTRNWKLPPLAAQYPIIGTLLEYRTVQKA
                     LTSYGENRIELINPMTKRLHADFRQIGAPTGRFACTNPNIQQVPHAVEYRRCFSGYPD
                     GRKLIIADYSQIELRILAEFSGDRGFIEAFNSGADLHRVTASQVFNAALDQVTKEQRD
                     FAKRLNFGVVYGIGAQRFAMMTGMNQPDAENVLRRYFGTYRQLDAYLREAANRAVSDR
                     HARTASGRLVRFRYDENDRQQISMTQRNGKNTPIQGTSADILKRALRLMKEELGETSA
                     RVVNIIHDEIVVEADAGEAESVAEKVERVMCAAGEEYLKTVPVKVETEIADEWVK"
     gene            complement(1021..>2141)
                     /locus_tag="VFO72_07715"
     CDS             complement(1021..>2141)
                     /locus_tag="VFO72_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9479213.1"
                     /translation="QLVIIHPFTINQLSFNGYALSLSTQQLLAVVMILLLTFINTRGL
                     TTGKIIQNTFTFTKTGALVGLILIGLFLGWNRNSAAYTSSWWAPWENGWKPTASQVWF
                     DPTSHGLLVLALLMLLGRAMVGPLFAQSAWNNVTFTAGEVRDPGKNLPRALLLGCGIV
                     VTLYLLANLAYVVTLPLAGIQNAPQNRVATAVMQAVLGPSGTVIMALAIMISTFGCNN
                     GLILAGARVYYAMARDGLFFKRVGSVNSHHVPAVALIAQGLWASFLVLPRTVTINPQT
                     GAYSFGNVYTQLLEYIVSADLILYVLMIGAVIVMRRKAPEIERPYRTFGYPIVPLIYI
                     IIAGLFIIALAILAPSTSGIGYLLVFTGIPIYFIWRRR"
BASE COUNT          533 a          612 c          581 g          415 t
ORIGIN      
        1 agcagctaac gcaggcgctc aatcgccttg gcattcccct tcccgactca actcgcaact
       61 ggaaactgcc accactggcg gcgcagtatc cgatcatcgg cacgctgctc gagtatcgca
      121 ccgtgcagaa ggcgctcacg agttacggcg aaaacaggat cgagttgatc aatcccatga
      181 ccaaacgcct gcacgccgac ttccggcaga taggcgcgcc gacggggcgc tttgcgtgta
      241 ctaacccgaa cattcagcaa gtgccacacg cagttgagta ccggcggtgt tttagtggtt
      301 atcctgatgg gcgaaagttg atcatcgctg actattcaca gatcgagttg cggatactgg
      361 cggagttttc cggtgatcgt ggtttcatcg aggcttttaa ctcgggcgcg gatctgcatc
      421 gcgttacggc gtcgcaagtc ttcaatgctg ctctcgatca agtcacgaaa gagcagcgcg
      481 actttgccaa gcgactgaac ttcggcgtcg tttatggaat cggcgcacaa cgctttgcga
      541 tgatgaccgg aatgaatcag ccagatgctg agaatgttct gcgccggtac ttcggcactt
      601 atcgtcaact cgatgcttac ctgcgtgagg cagcgaacag ggcggtgagt gatcgccacg
      661 cgcgcacggc gtcagggcgc ctcgtgcgtt ttcgttacga cgaaaacgat cgccagcaga
      721 tctcgatgac gcaacgcaac ggcaagaaca cgccgataca gggcaccagc gccgacattc
      781 tgaagcgtgc actcaggctg atgaaagaag agttaggcga gaccagcgcc agagtcgtca
      841 acatcattca cgacgagatt gttgtcgaag ccgacgccgg tgaagctgag tccgtagccg
      901 aaaaagtcga gcgcgtcatg tgcgccgcgg gcgaggaata cctcaagacc gtacccgtta
      961 aagtcgaaac cgagatcgcg gatgagtggg tcaaataaaa tccgcgtttt taatccgcgt
     1021 ttatctgcgt ctccaaatga agtagatcgg aatcccggtg aagacgagta aatatcctat
     1081 ccccgaagtc gacggcgcca ggattgccaa agcgatgatg aaaagcccgg caatgatgat
     1141 gtaaatcagc ggcacgatcg gatacccaaa cgttcgataa ggacgctcaa tctccggcgc
     1201 tttccgcctc atcacaatca ccgctccgat catcagcacg tacaaaatca gatccgcgga
     1261 aacaatgtat tcgagcagct gcgtgtaaac gtttccaaac gaatacgcgc ctgtctgtgg
     1321 attgatcgtg actgtgcgtg gcagcacgag gaacgacgcc cacagcccct gcgcaatcaa
     1381 cgccaccgcc ggcacgtgat gcgagttgac gctgccgact cgtttgaaga acaaaccatc
     1441 tctcgccatc gcgtaataaa cacgcgcgcc cgccagtatc agtccgttgt tacaaccaaa
     1501 agtcgagatc atgatcgcga gcgccataat gaccgtgcca ctgggaccaa gcaccgcttg
     1561 catcaccgca gttgccacac gattctgcgg cgcgttctga atccctgcga gtggcagtgt
     1621 caccacgtac gcaaggtttg cgagcagata cagcgtcacc acgatcccac aaccgagcaa
     1681 caacgcgcgt ggcaagttct ttcccggatc gcgaacctct ccggcagtaa aagtgacgtt
     1741 gttccatgcg gactgcgcga agagaggccc aaccatcgcc ctgcccagca gcatcagcag
     1801 cgccagcacg agcaggccgt ggctcgtcgg gtcaaaccaa acctgcgatg cagtcggttt
     1861 ccaaccattc tcccaaggcg cccaccaaga agaagtgtag gcggcactgt tgcggttcca
     1921 tcccaggaac aatccgatca aaatcaagcc gaccaatgcg ccggtcttcg taaaggtaaa
     1981 cgtgttctga atgatcttgc cggtcgttag cccgcgcgtg ttgatgaacg tgagaagcag
     2041 gatcataacc accgcgagga gttgctgtgt cgacaaactc aacgcatagc cattgaacga
     2101 aagttgattg atcgtgaacg gatggattat gacgagctgc g
//
LOCUS       DASTEY010000438         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_58946, whole genome shotgun sequence.
ACCESSION   DASTEY010000438 DASTEY010000000
VERSION     DASTEY010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58946"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2484
                     /locus_tag="VFO72_07720"
     CDS             <1..2484
                     /locus_tag="VFO72_07720"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9479214.1"
                     /translation="AYLAGKLSEARSMLLVDHTHECIPCRKAMKEARLRRTAVVKAPA
                     KTPRYSLQPVVMRWGIAAALVIGFGLLAVPFVQRYWPFGEFEATVQAAEGQVYQVADT
                     RTTAVSSGATLQKGERVRTAKDAHAVVRLGDGSLIEMKERSELYLTKSGQGTTIHLDR
                     GSIVVEAATQKDGKLFVESGDSLVSVMGTVFSVNNGTKGSRVSVIEGEVNLNHAGSDR
                     VLRPGEQATTSPAIATIPIKDEIAWSKNANRYASLLSGLATLKNELKTVSQPGVRNST
                     HLLDMMPENTVVYAALPNLTGTITESHRIIQERMSQNAALREWWEKEQSGRAQNMDQV
                     VETIRQFGSYLGDEIAVSVSMDEQGRPGEPLVLAEMTNSAGFREFLEQQIAKYAGVND
                     KPEIRFVEDPLKATGDDAGSKKEKLHIWIQNNLFAASPKLAQLQQLANVLNGGGISSF
                     TATPFRARIAQVYQEGAGIVVAANLEKVVAQTQAERAKGPDAEKRENALNQLGILSVK
                     YFVLDQKEAYGKTHTQASLSFNDTQRGIPSWLAAPGPMGSLEYISPDANVVAGFVVKN
                     PVALVDDLLGVLGTVSPELRQTLEKQQAARGLDVRNDIAAPLGGEFAFAIDGPILPTP
                     SWKLVFEVNDPAHLQQTLERAVSEVNKEAAKFGKSGLSWEQTALSGRTYYTLRAAGFG
                     FLEVNYTYANGYMIVGPSRALVERAIWSRESGSSLLRSAKFTAGLPADGNANFSAVFY
                     HNLGALVPAGLASTAQNHLPSGPQQAVKALAADMAPTLAYAYAQGDSITFAANTEGGP
                     FGLSPATLLGMPNALEMQSIIERGMRGKK"
     gene            2823..3215
                     /locus_tag="VFO72_07725"
     CDS             2823..3215
                     /locus_tag="VFO72_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479215.1"
                     /translation="MRLLSISLVSWLIGLLLLVATFSFMSQRGLSAADLNSATTTSLI
                     MAALLFSLAYAPSLSWLRRRMGGCKPALLFPLASAVVLNLPVFLIEILAIGRTLAPSE
                     ALACIGAFLLMGTVFGLGFVWKYQDAAL"
     gene            3223..>3506
                     /locus_tag="VFO72_07730"
     CDS             3223..>3506
                     /locus_tag="VFO72_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9479216.1"
                     /translation="MALPATVQEELIRPQTIDDPPVIDLHGLSVAFGRRQILKNLRGD
                     LRGKAIGLLGPNGAGKTTLIHTLLGFHHPSAGTAQIFGYDIVDQTKQIKSL"
BASE COUNT          740 a          944 c         1096 g          726 t
ORIGIN      
        1 gcttacctgg caggaaagct ttcggaagcg cggtcgatgt tgttggtgga ccacacgcat
       61 gaatgcatcc cctgccgtaa ggcgatgaag gaagcgcgtt tgcggcgaac ggctgtggtc
      121 aaggcgccgg ctaagactcc gcgctactcg cttcagcccg ttgtcatgcg ctggggcatt
      181 gccgctgcgc tcgtgatcgg tttcggtctg ttggccgtgc ctttcgtgca gcgctactgg
      241 ccatttggtg agtttgaagc gacggtccag gccgccgaag gtcaggtcta tcaagttgcc
      301 gacacgcgta ccacggccgt cagcagcggc gcgacgttgc aaaaaggtga aagggttcgc
      361 accgctaaag atgcgcatgc cgtggttcgt ctcggcgacg gctcgttgat tgaaatgaaa
      421 gagcgttccg aactctacct gacgaagagt gggcagggaa cgacgataca tctcgatcgc
      481 ggcagcatcg ttgtcgaagc ggcgacacag aaagacggca aattgtttgt cgagagtggt
      541 gattcgctgg tttcagtgat gggaacggtg ttctcggtca acaacggcac caagggctcg
      601 agagtttcgg taatcgaagg tgaagtaaac ctgaaccacg ccggcagcga tcgcgtgctg
      661 cgtccaggcg agcaggcgac gaccagtccg gcaattgcga cgattccaat taaggacgag
      721 attgcatgga gcaagaatgc taatcgctac gcgtcattgc tgtcgggttt ggcgacactc
      781 aagaacgagc tgaagacggt ctcgcagcct ggcgtacgca attcgacgca cctgctcgac
      841 atgatgcctg agaacacggt ggtctacgcc gcactgccca atctgaccgg caccatcact
      901 gagtcgcacc gcattattca ggagcggatg agccagaatg cggcgttgcg cgaatggtgg
      961 gaaaaggagc agtcgggtag agcgcaaaac atggaccagg tggtggagac gattcggcag
     1021 tttggatcgt acctgggtga tgagattgcg gtttcggttt cgatggatga acagggacgc
     1081 ccgggcgagc cgttagtgct tgcggaaatg acgaactccg ccggctttcg cgaatttctc
     1141 gaacagcaga tcgcgaagta tgccggtgtt aacgacaaac cggaaattcg ctttgtcgaa
     1201 gatccactta aggcaacggg agatgatgcc ggttcgaaga aagagaagct ccacatttgg
     1261 atccagaaca acttgtttgc cgcgagtccg aagttagcgc agcttcagca gctcgcaaac
     1321 gtgctgaatg gcggtggtat cagcagtttc actgccacgc cgttccgtgc tcggatcgcg
     1381 caggtttatc aggaaggcgc cggaatcgtt gttgccgcga acctcgagaa ggttgtggca
     1441 caaactcagg cggaacgcgc gaaaggcccg gatgctgaga aacgcgagaa cgcgttgaac
     1501 cagttgggaa ttctcagtgt gaagtatttc gtgctcgatc agaaggaggc gtacggcaag
     1561 acgcacacgc aggcttcgct ctccttcaac gacacgcaac gcggcattcc ttcgtggctg
     1621 gctgcgcctg gtccgatggg atcactcgag tacatttcgc cggatgcgaa cgtcgtggct
     1681 ggttttgtgg tgaagaatcc ggttgcgctg gttgacgatc tactcggcgt gctcggcacg
     1741 gtgtcgcctg agttgcgaca gactctcgaa aagcagcagg cggcgcgtgg actcgacgtc
     1801 cgtaacgata ttgccgcacc gttgggcggt gagtttgcct tcgcgattga tggtccaatt
     1861 ctgccgacgc cttcgtggaa gcttgtgttt gaagtgaacg atccggcgca tctgcaacaa
     1921 acgctcgagc gtgcggtgag tgaggtcaat aaagaggcgg ctaagttcgg taagagtggg
     1981 ctgagctggg agcagacagc gctcagtggc cgcacctact acacgctgag agcagcaggg
     2041 tttggcttcc tggaagtcaa ctacacctac gctaacggtt acatgattgt tggtccaagc
     2101 cgtgcgttgg ttgagcgggc tatctggtcg cgtgagagtg gttcgtcgtt gctgcggtcc
     2161 gcgaagttca cggccgggtt gccggcggat ggcaatgcaa acttctcggc agtgttctat
     2221 cacaacctcg gcgcgctggt gccggcaggt cttgcgagca ccgcccagaa ccatctgccg
     2281 agtggaccgc aacaggcagt gaaagcgctg gccgcggaca tggcgccgac gctggcgtat
     2341 gcctacgcac aaggcgacag catcacgttt gcggcgaaca cggaaggcgg tccattcggt
     2401 ttaagcccgg caaccctgct cggcatgccg aacgcgttgg agatgcagag catcatcgaa
     2461 cgcggcatga ggggaaagaa gtaggaccat gcgtaagcgt ggaattgatg agtgccgggg
     2521 gcgcggggtt cggttacaag tgcgatgagc gccggaggcg cggaagacgt tagccagggg
     2581 caagcgctgg gcgaagcgaa gcgcgccgcc cctggaacga ttgaaagatg gttacagctc
     2641 cgaaggagcg acagagctgt cgcaccttcg gtactcaatg gtttcctccg atagatccag
     2701 gggctgcgcg cgctccgctt tgcttcgcgc gctggctcct ggctacctta ttgacgcgcc
     2761 ttcggcgcgt tcgttgaaaa caccaagagc gcggcgcgcg aatgccgcgt gatactaacg
     2821 aaatgagact cctatcaatc tccctggtgt cgtggctgat cgggttgctg ctgcttgtcg
     2881 cgacgttctc tttcatgtcg caacgaggac tcagcgccgc tgatctcaac agcgccacta
     2941 ccacgtccct cataatggca gcgctgctgt tttcactcgc ctatgcgccg tcgctttcgt
     3001 ggctgcgacg acggatgggc ggttgcaaac cggcgctgct gtttccgctg gcttccgcgg
     3061 tcgtcctcaa cctcccagtg ttcctgatcg agatccttgc aattggcaga actttggcac
     3121 cctccgaagc gttagcatgc atcggtgcat ttctgctgat gggcactgtc ttcggactcg
     3181 gattcgtttg gaaatatcag gacgcagcgc tataagaaca ccatggcact ccccgcgacg
     3241 gttcaggaag aactgatcag accgcaaact attgacgacc cgccggtcat cgacctccac
     3301 gggctcagcg ttgcttttgg ccgccgtcaa atactcaaga acctgcgcgg cgatttacgc
     3361 ggcaaagcga tcggtttgct tggcccaaac ggcgccggca agaccacact gattcacacg
     3421 ctgctcggtt ttcatcatcc ctctgcgggc acagcacaga tcttcggtta cgacatcgtc
     3481 gatcagacaa aacagatcaa gtccct
//
LOCUS       DASTEY010000439         3358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_850083, whole genome shotgun sequence.
ACCESSION   DASTEY010000439 DASTEY010000000
VERSION     DASTEY010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850083"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFO72_07735"
     CDS             <1..869
                     /locus_tag="VFO72_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479217.1"
                     /translation="AAFGYVQVMNDYLPVGFRGLLLASFGAAYMSTIATQMNWGASYI
                     INDFYRRFVKPEASEKHYVFASRVATLIIVLLSIVVTYYMNQITSGWELVLALGAGTG
                     LVYILRWYWWRVNAWSEVAAMTSALIVSLTLRKVLSMPQFSEFHPSTPLGFAVQILVT
                     VGITTIVWLVATFATRPEPMEKLASFYRSVHPAGKGWRPVAEAAGIPERTGEVWPNLL
                     NWLMGVILVYSVLFGIGEFIFGTWSKVLLFFVIAAIAGGIMVWNLNRTGWAGLSESAE
                     PRVGNIVAEGAD"
     gene            869..1852
                     /locus_tag="VFO72_07740"
     CDS             869..1852
                     /locus_tag="VFO72_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9479218.1"
                     /translation="MSDKLRGDHDLVFAADLGGTYLRVALVDGSGKIHHQAKQRTPKG
                     DSPDSVVDALAAAAENWRKDDGPRIAAASIMVPGTVDNQNAVVVQAPNLPSLTNFPLK
                     AVLEERLGWPVLLENDANAAAVGEMWLGAARGCRDVVSVTLGTGVGGGVILEGELWRG
                     AHGSAGEIGHTTVDPFSGLKCKCGNVGCLELFASATAIVRMTRENLSRFSQSILNREE
                     LTAERVYDAGRDGDELALMVFQRFGSYLGIGLANLINIIDPEIIVIAGGAVNGWDLFA
                     ADMQREVSERAVRATAQQVTIAAAECGDNAGLLGAARLAFVRQDLHDFQDS"
     gene            1910..>3358
                     /locus_tag="VFO72_07745"
     CDS             1910..>3358
                     /locus_tag="VFO72_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HET9479219.1"
                     /translation="MTALIIHGHFYQPPRENPWTGEVERQPGAAPYHDWNERIHAECY
                     APNAVANNYERISFNFGPTLLSWLERHHPDTYQHVLAADRASAAQRGGHGNAIAQAYG
                     HAILPLCNERDRLTQVVWGMADFRFRFGREPEALWLPETAANDATLALLIDQGLRYVI
                     LSPEQAKRVRSNSEWIHVASGEIDTTRPYRFEHPDNSGRSIAVFFYDGPLARSVAFEQ
                     ALTSSRGLVELFLRASQRGDLVNVATDGETYGHHFKFGDLGLAHALGVDAKKAGFWIT
                     NYSEYLDRHPPETVVEIDNGPEGEGSSWSCAHGVGRWTRDCGCHTGGEAGWNQAWRDP
                     LRVAFNFLRDDAALKFESSGSELLRDPWAARNDYISVLLDHGRARDEFVARHALRPFS
                     EAEQVRVFQLLEMQHSALLMFTSCGWFFSDIAGIETVQVLRYAARVLDLQKRLGFEVP
                     RSQFLELLANAKSNRREKGNGADIFLRFAEPQI"
BASE COUNT          670 a          896 c         1020 g          772 t
ORIGIN      
        1 atgcagcgtt tggttacgtg caggtaatga acgattacct gcctgtcggt tttcgtggtc
       61 tattgctcgc ttcttttggt gctgcttaca tgagcacgat agcaacgcag atgaactggg
      121 gcgcgagtta cattatcaac gacttctacc ggcgttttgt aaagcctgaa gcgagcgaga
      181 agcattacgt gtttgcctcg cgagtggcga cgttaattat cgttctgctc tcgattgtcg
      241 ttacttacta tatgaaccag atcaccagcg gctgggagtt ggtgctcgcg ctgggagccg
      301 gcacgggact cgtttacatc ctgcgctggt actggtggcg tgtgaatgcg tggtcggaag
      361 tggcggcaat gacttcggca ctcatcgttt cactgacgtt gcgcaaggtt ctcagcatgc
      421 cgcagttcag cgaatttcat ccttccacac cactgggttt cgccgtacag attctggtga
      481 ctgtcggcat cactaccatc gtctggctgg tggcgacatt cgccacccgt cccgagccga
      541 tggaaaaact cgccagtttc tatcgcagcg tgcatccggc ggggaagggg tggcggccgg
      601 tggccgaggc tgcgggcatt ccggagcgaa cgggtgaagt ttggcccaac ttgctgaatt
      661 ggctcatggg cgtcattctc gtttactccg tcttgtttgg catcggcgag ttcatcttcg
      721 gcacctggtc caaagtgttg ttattcttcg tgatcgcggc gatagcgggt ggcattatgg
      781 tctggaactt gaaccgaacc ggctgggccg gactatcaga aagcgccgaa ccacgagtgg
      841 gcaacattgt tgctgaaggc gccgattgat gagcgacaaa ttaaggggag accacgacct
      901 ggtctttgcc gccgacttag gtggaacgta tctgcgagta gctctcgtcg atggctcggg
      961 aaaaatccat caccaggcaa aacagcgaac accgaaagga gattcgcccg attctgtcgt
     1021 cgatgctctg gctgccgcgg cggagaactg gcgcaaggac gatggtccac gcattgccgc
     1081 agcatcgatc atggtgcccg gcacagtcga caatcaaaat gccgtcgtgg tccaggcgcc
     1141 aaatcttccc tcgcttacga acttcccatt aaaagcagtg cttgaagaac ggcttggctg
     1201 gccggtgctt ctcgagaacg atgcgaatgc tgcggctgta ggcgagatgt ggttgggcgc
     1261 cgcgcgtggt tgtcgcgacg ttgtcagcgt aacgttggga acaggcgttg gtggcggtgt
     1321 gattctcgaa ggcgagctgt ggcgtggcgc gcatggttcg gcgggtgaga ttggccacac
     1381 gaccgtcgat ccgttctcgg gtttgaaatg taagtgtggc aacgtcggtt gtctcgagct
     1441 gtttgcctcg gcgacggcga tcgtacgcat gacgcgcgag aacttgtcgc gcttctcgca
     1501 gtcgatcttg aatcgtgaag aactgacggc ggaacgagtt tacgacgcgg gacgcgacgg
     1561 tgatgaactc gcgttgatgg ttttccagcg ttttggttct tacctcggaa tcggcctcgc
     1621 gaatctgatt aatatcatcg atcccgagat catcgtcatc gccggtggtg cggtgaacgg
     1681 ttgggacttg tttgccgccg acatgcagcg tgaagtcagc gagcgcgccg ttcgcgcgac
     1741 tgcgcaacag gtcacgatcg cggccgcaga gtgcggtgat aacgcgggac tgcttggcgc
     1801 cgcccggctc gcttttgttc gacaggattt acacgatttt caagatagtt agcgaggttt
     1861 ctcagtccca ctttcacaat cctgtaggtt ctgataatca tgtccaccta tgaccgcttt
     1921 aataattcac ggccacttct accaaccacc gcgcgagaac ccctggacag gcgaagtcga
     1981 acgccagccg ggagcagcgc cttatcacga ctggaacgaa cgcattcacg ccgaatgcta
     2041 tgcgccgaat gctgtggcga acaactacga gagaatcagt ttcaactttg gtccaacgct
     2101 gctctcgtgg ctcgaacgcc accacccaga cacgtaccag cacgtccttg cggctgatcg
     2161 agccagtgct gcgcaacgcg gcgggcacgg aaacgcgatc gcgcaagctt acggccacgc
     2221 gattctgccg ttgtgcaacg aacgcgaccg cttaacacag gtcgtttggg gtatggcaga
     2281 cttccgtttc cgtttcggcc gggaaccaga agcgttatgg ttgcctgaaa cagcggcgaa
     2341 cgacgccaca ctcgcgctgc tcatagatca gggtctacgt tacgtaatcc tctctcccga
     2401 acaggcgaaa cgcgtgcgct ctaactccga atggattcac gtggcgagcg gcgagatcga
     2461 cacaacccgc ccctatcgct tcgagcaccc tgataactcc ggtcgctcaa tcgcggtttt
     2521 cttttacgac ggcccactcg cgcgctccgt cgcatttgag caggcgttga cttccagccg
     2581 cggtctcgtc gaactcttcc tgcgcgcctc acagcgcggc gatctcgtga acgtagcgac
     2641 ggacggcgaa acgtacggtc atcatttcaa gtttggcgat ctgggtctcg cgcacgcgct
     2701 tggcgttgac gcaaagaagg cgggattctg gatcacaaat tacagcgagt atctcgatcg
     2761 tcatccaccg gaaacggtag tcgagatcga taacggtccg gaaggtgagg gaagttcgtg
     2821 gagttgtgcg catggcgtcg ggcgttggac acgcgactgc ggttgtcaca cgggtggtga
     2881 agctggttgg aaccaggcgt ggcgcgatcc tttgcgtgtt gcgtttaact tcctgcgtga
     2941 cgacgcggcg ttgaagtttg agagcagtgg aagtgaactg ttacgcgatc cgtgggcggc
     3001 gcgaaacgac tatatctctg tgctgttgga ccacgggcgt gcgcgcgacg aatttgtagc
     3061 tcgccacgct ctccgcccgt tcagtgaagc agagcaggtg cgagtgtttc agttgttgga
     3121 gatgcagcat agcgcgttac tgatgtttac aagttgcggc tggttctttt ctgacattgc
     3181 cgggatcgaa acggttcagg tgttacgtta cgcagcccga gtgctcgatt tgcaaaagcg
     3241 gttgggcttc gaagtgccgc gcagtcagtt tctcgagtta ctggcgaacg caaagagtaa
     3301 ccggcgcgag aaaggaaacg gcgccgacat ctttctgcgt tttgccgaac cccaaatt
//
LOCUS       DASTEY010000440         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_932468, whole genome shotgun sequence.
ACCESSION   DASTEY010000440 DASTEY010000000
VERSION     DASTEY010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932468"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VFO72_07750"
     CDS             <1..1214
                     /locus_tag="VFO72_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9479220.1"
                     /translation="IVLATDRLNRIKSVASNEDGSGRAVAEAGVILSDFQRAVDQKGL
                     LYPPDPTERGCFLGGTVATNASGARTFKYGPTRNYIERLKLVLASGEILDLRRGDKFP
                     QIDLPHYRLPATRKNATGYFIALGMDAIDLFIGSEGTLAVICEIEARLVPKPQGLLSG
                     VVFFAQQADVLSFVANARVALDARALEFFDNESLIFLREKYPEIPAGAVGAIFFEQET
                     TDETEETILNQWIALLDEHHAFADSWFATNEQDQARLREFRHQLPVLMNEWFARYRQQ
                     KVSTDMAVPDDAFAALFRLYQETLRPSGLRYTIFGHIGDNHVHVNILPRDEDEAARAR
                     ELYVRFLKYAASVGGTLSAEHGVGKLKRDYLRVFYSDDQLREMAALKKAFDPHGILGR
                     GNIFAEDFLDA"
     gene            1207..2376
                     /gene="aroC"
                     /locus_tag="VFO72_07755"
     CDS             1207..2376
                     /gene="aroC"
                     /locus_tag="VFO72_07755"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF003793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9479221.1"
                     /translation="MLRFTTAGESHGRALVAIVEGLPAGVPVNQELIDYELRRRQLGY
                     GRGGRMKIERDHAEILTGVRHGLTLGSPLTLLIENKDWSNWTEVMATEPREIAPEKSR
                     RLKRPRPGHADLPGGLKYDVRDLRNILERASARETTARVACGALAKQLLACFGVEVRS
                     HVIQLGGIPDKPLELTWAQIAAIADDAPLHCADAEAEQKMMALIDETRHRGDTLGGIF
                     EVAARGAVPGLGSHTSWDVKLDGLLAQAIMSIPAVKAVSIGAGAEASSLPGSEVHDEI
                     AYSRETREFIRETNRAGGLEGGITNGEEIRVRGHLKPLSTLRRALRSVDIDTKEEEAA
                     AFERSDVTAVPAAGVIGESMVALMLARSMREKFGGDSLGEMKRNFEGYREQLRGY"
BASE COUNT          512 a          682 c          786 g          520 t
ORIGIN      
        1 ggatcgtgtt ggccacagat cgactcaacc gcataaaaag cgtggcttcg aacgaagacg
       61 gatctggacg cgctgtcgcc gaggccggtg tgatcttgtc cgacttccaa cgtgccgtag
      121 atcagaaggg actcctgtac ccgccggacc cgacggagcg tggctgtttt cttggcggca
      181 cggttgcgac caatgcgtcg ggcgcgcgca ctttcaaata tggtccaact cgcaactaca
      241 tcgagcgttt gaaactggtg ctcgcttcgg gggagattct ggatttacgc cgcggtgaca
      301 agttcccgca aatcgatctt cctcactacc gtctgcctgc gacacgcaaa aacgccaccg
      361 gctatttcat cgcactcggc atggacgcga tcgacctctt catcggcagc gaaggcacac
      421 tcgcagtgat ttgcgagatc gaagcgcgcc tggtgccaaa gccgcagggc ctgttgagcg
      481 gcgtcgtctt ctttgcgcaa caagctgacg tgctctcgtt tgtcgcgaac gcgcgcgtcg
      541 ctctcgacgc acgcgcgctc gagtttttcg ataacgagtc gcttatcttt ttgcgcgaaa
      601 aatatccgga aatacccgcc ggagccgtgg gcgcgatatt tttcgaacag gagaccacgg
      661 acgaaacgga ggaaacgatt ctgaatcaat ggatcgcgct attagatgag catcatgctt
      721 ttgcagattc gtggtttgcg acgaacgaac aggaccaggc acgattgcgt gagtttcgcc
      781 atcaactgcc ggtgctgatg aacgagtggt ttgcgcgcta ccggcagcaa aaagtgtcga
      841 cggacatggc tgtgcctgac gatgcgtttg cagcgttgtt tcgtctgtac caggagacgt
      901 tgcgtcccag cggattgcgt tacacgatct tcggccacat cggtgacaat cacgtccacg
      961 tcaacatcct gccgcgtgac gaagacgaag ccgcacgagc gcgcgagttg tatgttcgtt
     1021 ttctgaaata cgcggcttca gttggcggaa cgctgtcggc agagcacgga gtcggaaagt
     1081 tgaaacgaga ttatctgcgg gtgttttata gcgacgatca gctccgcgaa atggccgcac
     1141 tgaagaaggc cttcgatcct catggtatct tgggcagagg aaacatcttc gccgaggact
     1201 ttttggatgc ttagattcac cacagccgga gaatctcacg ggcgcgcgct ggtcgcgatt
     1261 gtcgaaggcc tgcccgcagg tgtgccggtc aatcaggaac tgattgacta cgagttgcgt
     1321 cgacggcagc tcggttatgg ccgcggcggt cgcatgaaga tcgagcgcga ccatgccgaa
     1381 atcctgaccg gcgtgcgcca cggtctcaca ctcggctcgc cactcacgtt gctgatcgaa
     1441 aacaaagatt ggtccaactg gaccgaagtg atggccactg aaccgcgaga gatcgcgccg
     1501 gaaaagtcgc ggcgtttgaa acggccgcgt ccaggtcacg ccgatcttcc cggcggcttg
     1561 aagtatgacg tgcgcgactt gcgcaacatt ctcgaacgcg cttcggctcg cgaaacgacg
     1621 gcgcgagttg cgtgcggcgc gcttgcgaaa cagttgctgg cctgctttgg cgtcgaagtg
     1681 cgcagtcacg tgattcaact cggcggcata cctgacaagc ctctcgaatt aacgtgggcg
     1741 cagatcgccg cgatagctga cgacgcgccg ctgcactgtg ccgacgctga agctgagcaa
     1801 aaaatgatgg cgctgattga cgaaacgcgc catcgtggcg acacgctcgg cgggatcttt
     1861 gaagttgcgg ctcgtggcgc ggttcccggt ttgggttcgc atacgtcttg ggacgtgaag
     1921 ctcgatggac tgctcgcgca ggccattatg tcaattccgg cggtgaaggc agtgtcgatt
     1981 ggcgccggcg ccgaagccag cagtttgccc ggttctgagg ttcacgacga gatcgcttac
     2041 agcagggaga ctcgcgaatt tattcgcgag acgaaccgcg cgggcgggct cgaaggcggc
     2101 attacaaatg gagaagagat tcgcgttcgc ggccatttga aaccgctgtc cacgctgcgt
     2161 cgcgcgctgc gatcggtgga catcgacacg aaggaagaag aagcggccgc gtttgaacgt
     2221 tctgatgtga cggcggtgcc tgcggcgggt gtgattggcg agtcgatggt ggcgctgatg
     2281 ctggcacgtt ccatgcgcga gaagtttggc ggcgacagtc tcggcgagat gaagcgcaac
     2341 ttcgaaggct accgcgagca gttgcgaggg tattagcttt cattctcacc cggcttcagc
     2401 cgggtgccgc gggtttctgg ccgagaggaa accgtttaaa cggtttcccg cacgggcgct
     2461 ttgctgttca cccggctgaa gccgggtcag aatgagaggg
//
LOCUS       DASTEY010000441         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1361088, whole genome shotgun sequence.
ACCESSION   DASTEY010000441 DASTEY010000000
VERSION     DASTEY010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361088"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1487)
                     /gene="aceA"
                     /locus_tag="VFO72_07760"
     CDS             complement(168..1487)
                     /gene="aceA"
                     /locus_tag="VFO72_07760"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET9479222.1"
                     /translation="MKTYDHVALENEWATSARWQGIKRGYTAEDVLKLRGSLEIKYTL
                     AERGAKRLWELLHSEPYVPALGAMTGCQAVQQVQAGLKAIYLSGWQVAADANLAGHMY
                     PDQSLYPANSVPEVVRKINHALQRADQIDHAAGRNGIDWYAPIVADAEAGFGGNLNAF
                     ELMKAMIEAGAAAVHFEDQLASCKKCGHMGGKVLVPTQEFIQKLIAARLAADVLGVDT
                     ILVARTDADGAGLLTSDIDERDHAFLTGERTAEGFFGVKAGLDQAIARALAYAPYADI
                     VWCETSEPNLEDASRFAHAIHEQFPGKLLAYNCSPSFNWKKKLDDHTIAKFQKELGLM
                     GYKFQFITLAGFHALNLAMFELAREYRDSGMSAYAKLQATEFEREADSNYRAVKHQAF
                     VGTGYFDTIAQVIAGGNSSTTALNGSTEQEQFTAPVLHQTATAVSQG"
     gene            complement(1519..>2961)
                     /locus_tag="VFO72_07765"
     CDS             complement(1519..>2961)
                     /locus_tag="VFO72_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479223.1"
                     /translation="LHQELTPRQQSLTRKRAAVLSAAHQGQKPNYLPPSEATSTGWTI
                     PLPEWCADQRNQMTGPADDAELVVKMLNSGAPGVMLDLEDSMANTWSNLKTGLENIRA
                     ALKGELTYFDVKRNRTAGINESNTVVFVRPRGLHISQAGVVKDELMSASLFDVAMVAY
                     EADMDRLRHPLSFYIPKSESAEEALWWRDLFRAIEDAKRLPRGYIKCMALVESHPIAY
                     QMEEFIYNVRDHIIGLNLGRWDYMASLIHFNIEDPEWILPDRNTIPHDVPFFQNLREL
                     LAETCHKHGILAIGGMTALYPSRTDPELNERALRVLAADKKNEANCLMDGAWTGHPDQ
                     NEIAVKQFPFPNQITARKPGAPAHPDLRPSPKGVGNITEAGTRAAIRTVIRYRNGVLN
                     GKGASLLDGYMEDLATDRIYRLMIAQRLRHQVQEAGATPHSITEMFDEELDRILQELP
                     PNTDAPVVENYRTARHLSESLIRNQEFDPI"
BASE COUNT          595 a          844 c          839 g          683 t
ORIGIN      
        1 tgagaatgaa agaagaattt tgaggcaaag cctttgcgaa cccaggcagg cgagacgcct
       61 gcggtcccag gaacagacgc gtcattccaa acggttaaga taaagatgta aaagatacga
      121 agattgactc aaccgattcc aggaacagac gcgtcattcc tgacgggtta cccctgactt
      181 accgctgtcg ctgtctgatg cagaacgggc gcagtgaatt gttcctgctc tgtcgatcca
      241 ttgagcgcgg tcgtcgatga attgccaccc gcgatgacct gcgcaatcgt gtcgaagtaa
      301 ccggtgccca caaacgcctg gtgcttcacc gcgcggtagt tgctatcagc ttcgcgttcg
      361 aattcggtgg cctgaagttt cgcataagcg ctcatgcccg aatcgcggta ttctcgcgcg
      421 agttcaaaca tcgccaggtt tagcgcgtgg aaaccggcaa gcgtgatgaa ctgaaacttg
      481 tagcccatca accccaactc tttttgaaac ttcgcaatcg tgtggtcgtc gagtttcttc
      541 ttccagttga acgaaggcga acagttgtag gcgagcagct tgcccggaaa ctgctcgtga
      601 atggcatggg caaaccggct cgcgtcctcg aggttcggtt cggaagtttc gcaccatacg
      661 atatctgcat acggagcgta agcgagggcg cgcgcgatcg cctggtccaa accggctttg
      721 acgccgaaga acccttccgc cgtgcgttcg ccggtcagaa acgcatgatc gcgttcgtcg
      781 atgtcgctcg tgagtagacc agcgccgtca gcgtccgtac gagcaacgag aatcgtgtcc
      841 accccgagaa cgtcagccgc cagtcgggcg gcgatcagtt tctgaatgaa ctcctgcgtc
      901 ggcaccagga cctttccgcc catgtgtccg cacttcttgc acgacgccag ttgatcttcg
      961 aagtgcaccg cagcggcgcc cgcttcgatc atcgccttca tcaactcgaa tgcattcagg
     1021 ttgccaccaa agcccgcttc ggcgtcagcc acgatcggcg cataccaatc gattccgttg
     1081 cggccggcgg cgtgatcgat ctgatcggcg cgttgcagcg cgtggttgat cttgcgcacc
     1141 acttccggaa cgctgttggc cggatacaaa ctttgatcgg gatacatgtg accggcgagg
     1201 ttcgcgtcgg ccgccacttg ccagccgctc aagtagatcg cctttaaccc ggcctgcact
     1261 tgttgcactg cctgacagcc ggtcatcgcc ccgagcgcag gaacataagg ttcgctatgc
     1321 agcagttccc aaagacgttt ggcgccgcgt tcagccagcg tgtacttgat ctcgagagat
     1381 ccgcgcagct ttagcacgtc ttcggcggta tagccgcgtt tgattccttg ccagcgagcg
     1441 ctcgtggccc attcgttttc cagtgcaaca tgatcgtaag ttttcatcgt tcccctcccc
     1501 ggatagatct tgcagttgtc agattggatc aaactcctgg tttcgaatca gcgactcact
     1561 gaggtgccgg gccgttcgat aattctcaac gaccggcgcg tcagtgtttg ggggaagttc
     1621 ttgcaaaatg cgatctaatt cttcgtcgaa catttcggta attgaatgcg gtgtggcgcc
     1681 tgcctcctgg acctggtgac gaaggcgttg agcgatcatg agacggtaaa tgcgatctgt
     1741 cgcgaggtct tccatgtagc cgtcgagcaa actggcgcct ttgccgttca gcacaccgtt
     1801 gcgatagcga atgactgtcc gaatcgccgc gcgtgttccg gcttcagtga tgtttccgac
     1861 tcccttcgga ctcggccgca gatccggatg ggcaggtgct cccggtttac gtgcggtgat
     1921 ctggttcgga aacgggaact gcttcacggc gatctcgttc tggtcaggat gtccggtcca
     1981 cgcgccatcc attaaacagt tcgcttcgtt cttcttatct gctgcgagta cgcgcagtgc
     2041 gcgttcgttg agttccggat ctgttcggct gggatagaga gccgtcatgc cgccaatcgc
     2101 gaggatgcca tgcttgtggc acgtttcggc cagcaattct cgcaggtttt ggaagaacgg
     2161 aacgtcgtgc ggaatcgtgt ttcggtcagg caggatccac tccggatctt cgatgttgaa
     2221 gtggatcaaa ctcgccatgt aatcccagcg accaagattg aggccgatga tgtggtcgcg
     2281 cacgttgtag atgaactcct ccatctgata agcaatcgga tgcgattcca ccaacgccat
     2341 gcacttgatg tagccacgcg gcaacctctt ggcgtcttca atcgcgcgaa acagatcgcg
     2401 ccaccacaat gcctcttccg ctgattccga cttcggaatg taaaaactga gcggatgccg
     2461 cagccgatcc atgtcagcct cgtaagcaac catggcgacg tcgaacaatg aagccgacat
     2521 caactcatct ttgactactc ctgcctggct gatgtgcaga ccacgcggac gaacgaacac
     2581 cacggtgttg ctttcgttga tcccagcggt gcgattacgt ttcacgtcga agtaagtgag
     2641 ttcgcctttc aaagccgcgc ggatgttctc gaggccggtt ttcaggttgg accacgtgtt
     2701 ggccatcgag tcttcgaggt ccaacatgac gccgggcgcg cccgagttca acattttaac
     2761 gaccagttcc gcgtcgtctg ccggcccggt catctggttg cgctgatctg cgcaccattc
     2821 gggcagcggg atcgtccagc cggtggaagt cgcctcggaa ggtggcaggt agttgggctt
     2881 ctgaccttga tgggccgctg aaaggacggc ggcgcgcttg cgagtgagtg actgttgtcg
     2941 cggcgtcaat tcctgatgaa g
//
LOCUS       DASTEY010000442         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1147142, whole genome shotgun sequence.
ACCESSION   DASTEY010000442 DASTEY010000000
VERSION     DASTEY010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147142"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..613)
                     /locus_tag="VFO72_07770"
     CDS             complement(134..613)
                     /locus_tag="VFO72_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET9479224.1"
                     /translation="MILSREELVTRIDAERKKGSRVVLANGCFDVLHVGHVRYLAGAR
                     ELGDVLVVGINSDAQVERLKGPGRPVLPASERAEIVAAIESVTYVTVFDEPTVEELLL
                     TLKPDVHAKGTDYTIDSVPERDVVRSYGGRVAIVGDPKDHSTSAILSRLGGNVEKLS"
     gene            complement(597..1595)
                     /locus_tag="VFO72_07775"
     CDS             complement(597..1595)
                     /locus_tag="VFO72_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HET9479225.1"
                     /translation="MLQERLLKIIQRFPEQKLLIVGDTIADKFLYGSISRVSREAPVF
                     ILRHESTETVPGGAANCALNLAALGANVSLVSIAGNDEAGAALRDKLSVAGVNVQGLL
                     LSDEIQTTTKLRILAGHSHANKQQVIRVDYEDAPLNNAGLRDSINAKLKSSLSAAGAV
                     ILSDYNYGLVDQQTIELMRETQQAKKIPVLVDSRSRLSDFSGFTAATPNQDEVEHLIG
                     AAVESTEKLEMAAGRLQEELQLEALLVTRGSRGMMLLTSNAKPLHIAAVGAQAVDGTG
                     AGDTVIATFALGLASGATFEEAAQLANYAGGLVVMKRGTASVTAAELEQAINDSKP"
     gene            complement(1573..2163)
                     /locus_tag="VFO72_07780"
     CDS             complement(1573..2163)
                     /locus_tag="VFO72_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9479226.1"
                     /translation="MKRPALFMDRDGTISEEVGYVNHLSRFRLFPYTAEAIRLLNEHG
                     WLAIVVTNQAGVARGYFSEDVIVQIHEQLRRSLQNASAKLDAIYYCAHHPSVGEPPYR
                     LDCDCRKPKTGLITRATTDFEIDLERSWMVGDRYGDVELARNAGLHSAFVLSGYGRGE
                     WEYQRSAWKLEPEIVAENLLEAAKIIIERDAARATA"
     gene            complement(2168..3268)
                     /gene="waaF"
                     /locus_tag="VFO72_07785"
     CDS             complement(2168..3268)
                     /gene="waaF"
                     /locus_tag="VFO72_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET9479227.1"
                     /translation="MSSVVQANTIRRVVVRGTNWVGDSVMTLPALRALRRVLPAAKIT
                     LVVRPGAKGIFAEADFVDELLVYERRNAWSVVPQIKEWRRCEFDLAVLFQNAFEAALI
                     PFLAGVPLRLGYATESRQALLTHPLPLPDWRSSRHEVFYYLYLVTALEQLLFGTSTIC
                     ESEPDASLNISEARKSEAENLLRAYGVREREPVVVLCPGSINSRAKRWPAEDYAALAD
                     RLIDSSRQVLLIGSADEAEVTREVTTRMRQQPVVLTGKTSLSEITAVLSLVDLVVTND
                     TGPAHIAAALGRPTLVIFGPTNPSTTRPFAPAAEIVRHPPDCAPCMLRDCPIDHRCMT
                     AITVDEVFERSQALLKRASFAKTVRSLCDSVV"
     gene            complement(3273..>3702)
                     /locus_tag="VFO72_07790"
     CDS             complement(3273..>3702)
                     /locus_tag="VFO72_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9479228.1"
                     /translation="FRACIFRMSTVFARPSEGNDFGFVTHYVESIKHRQPIRLPAGVD
                     PIRDILYVDDLSRACRAFMKSSFPFGLYNLGGGRLNAVSLRGIIERVSKMIEIEPVIE
                     EDPTLAVPAPLRYVSDLTRIGDELGWRPQIGIDDGLRVIL"
BASE COUNT          856 a         1118 c          961 g          767 t
ORIGIN      
        1 tattccacca aaatccaaaa atccgttcgg cggttcgcct ataagccaga aagatccgtc
       61 agggttgaat cgtctgccct ggctcaacgc aggtatcgac acagcaagca acaaactcgc
      121 cattgctact gctttaggaa agtttctcaa cgttacctcc gagccgagac agaatggccg
      181 aagtcgaatg atctttcggg tcacctacga ttgctacgcg gccgccgtag gatcgtacca
      241 cgtcccgttc cggcacagag tcaatcgtgt agtcggtccc cttggcgtgc acgtccggtt
      301 tgagagttaa caacagttct tcaacagtcg gctcatcaaa aactgtgaca taagtcacag
      361 attcgatcgc ggcaacaatc tcggcgcgct cgctcgccgg aagaaccggc cggcccggtc
      421 ctttgagtcg ctccacttgt gcatccgaat taataccgac aaccaaaaca tctccgagtt
      481 cgcgtgctcc cgccagataa cgcacatgcc ccacgtgcaa cacgtcgaaa caaccgtttg
      541 ccagcacaac gcgcgaaccc ttcttccgct cggcgtcgat ccgcgtcacc agttcttcac
      601 ggcttagaat cattgattgc ttgctcgagt tccgctgctg tcactgaggc tgtgccgcgt
      661 ttcataacga caaggccgcc ggcatagttg gccagttgtg ctgcctcctc gaaagtcgca
      721 ccagaggcga gacccaaagc gaacgtggcg atcacggtat cacccgcgcc cgttccgtcg
      781 accgcttgcg ccccaacggc ggcgatgtgt aacggcttcg cgttgctggt cagcaacatc
      841 attccgcgac taccgcgagt tacgagtaga gcctcaagtt gcagctcctc ctgcaaacgg
      901 ccggctgcca tttctaattt ttcggtcgat tcaactgcag ccccaatcaa atgttcgact
      961 tcgtcttgat tcggcgtggc cgcagtgaag ccggaaaaat ccgacaagcg agagcgcgaa
     1021 tcgacgagca ccggaatctt ctttgcttgt tgtgtttcgc gcatcaactc gatcgtctgt
     1081 tgatcgacga gaccgtagtt atagtcggaa agaattaccg caccggcggc gctgagggaa
     1141 ctcttcaatt tcgcgtttat tgaatcgcgc aaaccggcgt tatttaaagg agcatcttcg
     1201 tagtcgacgc ggatgacctg ctgcttgttg gcatgagaat ggcccgcgag aattcgtaac
     1261 ttcgtcgtcg tctgaatctc gtctgaaagc agcagcccct ggacattcac tccggcaacg
     1321 ctgagtttgt cacgcaacgc cgcgcccgct tcgtcgttac cggctattga cacgagtgaa
     1381 acattcgcgc ccagtgccgc gagattcaaa gcacaattcg cagccccgcc gggaacggtc
     1441 tcagtcgact cgtgacgcaa aataaaaacc ggcgcctcgc gcgacactct gctgatcgaa
     1501 ccgtaaagaa acttatcggc aatagtgtcg ccaacgatca gcagcttttg ctcggggaag
     1561 cgctgaatga ttttaagcag tcgctcttgc agcatcgcgc tcgataatta tttttgccgc
     1621 ctccaacaga ttttcagcaa cgatctccgg ctccagcttc caggcgctgc gttgatactc
     1681 ccactcgccg cggccatatc cgctcaacac aaacgcggaa tgcaatcccg catttctcgc
     1741 gagttccacg tcgccatagc gatcgcccac catccagctc cgttccaaat cgatttcgaa
     1801 gtccgtagtg gcgcgagtaa tcaggcccgt tttcggcttg cggcagtcgc agtctaatcg
     1861 ataaggcggc tctccgacag acgggtgatg cgcgcagtaa tagatggcgt cgagcttggc
     1921 ggaggcattc tgcaaacttc gacgcagttg ctcgtgaatc tgcacgatga cgtcttcgga
     1981 gaagtaaccg cgtgccacac cggcctggtt ggttacgaca atcgccagcc acccgtgttc
     2041 attcaacagc ctgatcgctt cggccgtata cggaaacagg cgaaaacgag aaagatggtt
     2101 tacgtaccca acttcctcgg agatcgttcc gtcgcgatcc atgaaaagtg cgggacgctt
     2161 catctgttca cacgactgaa tcgcaaaggc tcctcacggt tttggcaaag ctggcgcgtt
     2221 tgagtagagc ctgagaacgc tcgaacacct catcaacggt gatggctgtc atgcaacgat
     2281 ggtcgatcgg gcagtcgcgc aacatgcaag gcgcgcagtc gggcgggtgg cgcacgattt
     2341 ccgctgcggg cgcaaatggt cgcgttgtgg acggatttgt tggtccaaag atcacgagtg
     2401 tcggtcggcc aagtgcggcc gcgatgtgcg ccggtcccgt gtcgttggtg acgaccagat
     2461 cgacgagcga aagcacggcc gtgatctcgc ttagcgaggt ctttccggtt aacacgaccg
     2521 gctgttgtcg catacgcgta gttacttcgc gcgtgacttc tgcttcgtca gccgaaccga
     2581 tcagcaacac ctgccggctc gagtcgataa gtcgatcggc caaagcagca tagtcttcgg
     2641 cgggccaacg cttggcccgg ctgttaatcg aacccggaca cagaacaacg accggttctc
     2701 tttcgcgcac accatacgcg cgcagcaagt tctccgcttc ggacttgcga gcttcagaga
     2761 tatttagact cgcgtcgggt tcgctctcgc aaatcgtgct ggtgccaaac agcagttgct
     2821 cgagcgcggt gaccaaatag aggtaataaa aaacttcatg ccgcgacgaa cgccaatccg
     2881 gcaacggtag cggatgcgtc aacaacgcct gccgtgactc agtcgcgtag ccaagccgca
     2941 gcggtacgcc cgccaggaag ggaattagcg ctgcctcaaa agcattttga aacagcaccg
     3001 ccagatcaaa ctcgcaccgc cgccattctt tgatctgcgg cactaccgac catgcgttgc
     3061 gccgctcata aaccaaaagc tcgtcaacga aatcagcttc tgcaaagatc ccctttgcac
     3121 cgggccgcac gaccagcgtg atcttcgctg caggcagcac gcgtcgcagc gcacgcaacg
     3181 cgggcaaagt catgaccgag tcgccgaccc aattcgtgcc gcgcacgacg actcgtctta
     3241 tcgtattcgc ctgaaccacc gaactcataa aactacagaa tcactcgtag cccatcgtca
     3301 attccgatct gcggacgcca gcccaactca tcaccgattc gtgttaagtc cgagacgtag
     3361 cgcaaaggcg ccggaacagc cagagttgga tcttcttcaa tgaccggctc gatctcaatc
     3421 atcttggaga cgcgctcaat aatcccgcgc aagctcaccg catttaaccg cccgccgccg
     3481 aggttataga gtccaaatgg aaacgacgat ttcataaacg cgcgacaggc gcgcgacaag
     3541 tcatcaacgt acagaatatc gcgaatcgga tcgacgccgg caggaagccg aatcggttgc
     3601 cggtgcttga tcgactccac gtagtgcgtg acaaacccaa agtcgtttcc ttcagacggc
     3661 cgcgcaaaaa ctgtcgacat gcgaaaaata caggcgcgaa ag
//
LOCUS       DASTEY010000443         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_834134, whole genome shotgun sequence.
ACCESSION   DASTEY010000443 DASTEY010000000
VERSION     DASTEY010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834134"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFO72_07795"
     CDS             complement(<1..1034)
                     /locus_tag="VFO72_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9479229.1"
                     /translation="MKVFRNYINGEWAESKSGKTFPNINPANTDDTIGLFQASNADDV
                     NDACAAAVAAQTAWAEMPATRRGEYLFKAAELLEARLPQLGEEMTREEGKTLPEAVGE
                     VKRAINIFRYFAGEGSRQFTYQVPSERENVICYTIRKPLGTVALITPWNFPSAIPAWK
                     LAPALVAGNAVVLKPASLAPLSSYRLVEALHEAGIPRGVLNYVTGTGGAAGDPLTSHQ
                     AIKAVSFTGSTSVGNALYKKVSERKIRVQLEMGGKNPTIVLADADLDYAADTLINGAF
                     FSTGQKCTACSRAVIERSIYEPLVDKLIEKTRKLRVGNGLEQGVQIGPAVDEDQLNTD
                     LEYIEIAKQEG"
     gene            complement(1127..2224)
                     /locus_tag="VFO72_07800"
     CDS             complement(1127..2224)
                     /locus_tag="VFO72_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9479230.1"
                     /translation="MKTSRAAVLTNYEAPLELKEFPIPESIEPGAALVKVKLAGICGT
                     DVHLWHGQLPIPLPVILGHETVGVIEEVGEGLTHDWSNNPIAAGDRITWSSSINCNEC
                     YYCRVKPEPTRCLKRKAYGISYDCSVAPHLFGGYADYIYLRPGTAIFKIPEQLPTEAV
                     VGAGCALVTSLHGIERIGVRMGDNVVIQGSGPVGLACLAVAKAGGAAQIIIFGGPPHR
                     LQLATRFGADVSIDVDASSVEERKARVMELTGGFGADVVLECVGIPQAVTEGVELCRD
                     GGRYLVLGHYGNAGTIPFNPHIVTRKQLTMAGSWGFEPRHTQAALKFLSRTRDQFPFE
                     QLVSTPFPLDRAFDALQATASWSTAKSAIAP"
     gene            complement(2337..2738)
                     /locus_tag="VFO72_07805"
     CDS             complement(2337..2738)
                     /locus_tag="VFO72_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9479231.1"
                     /translation="MTHITKISLEFPAAVPEIPVSDMNAALDYYQHKLGFNIDWGGAG
                     GIAGISKGHCRMFLTDRDFREHRGNASPVLIWLNLNSKKEVEELYEIWNASGAKIISP
                     PESKPWKLHEFTVSDLDGNLFRVFYDFSNDT"
     gene            complement(2790..2978)
                     /locus_tag="VFO72_07810"
     CDS             complement(2790..2978)
                     /locus_tag="VFO72_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479232.1"
                     /translation="MSEQQSQKSGVRSEAQKTDRARDGYNPIPPANPVAGAFGDRERD
                     TPTDQEVALDKAVNERSE"
     gene            complement(2992..>3510)
                     /locus_tag="VFO72_07815"
     CDS             complement(2992..>3510)
                     /locus_tag="VFO72_07815"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9479233.1"
                     /translation="RLSSEQPDDSVLVDCVRAKIGRIVSHPAAINVSAQDGHVTLDGP
                     ILAQEVPHLLSCVKWIHGVKDINNNLEVHEEAGNHPALQGGRERQGSRFELFQDNWSP
                     TARLLAGAAGASLAAYGGMRRSALGAGLGAAGLLLTTRAITNTNFSDLANQASKQLHI
                     GGSSTANVQPTA"
BASE COUNT          723 a         1005 c          970 g          812 t
ORIGIN      
        1 ccctcttgct tggcgatctc gatgtactcg agatccgtgt taagttgatc ttcgtcaact
       61 gccggcccaa tctgcacgcc ttgttcgaga ccattgccga ctcttagttt gcgagtcttc
      121 tctatcagtt tgtcgacgag cggctcgtag atcgatcgtt cgatcactgc acgactgcac
      181 gccgtacact tctgtcccgt tgaaaagaac gcgccgttga tgagcgtgtc tgccgcgtag
      241 tcaagatccg cgtcggcgag aacgatggtc ggattcttcc cgcccatctc gagctgaacc
      301 cgaatcttgc gttcagaaac ttttttatac agcgcgtttc cgaccgaggt tgaaccggta
      361 aacgaaactg ctttgatcgc ttgatgactc gttagcgggt cgccggcagc accgccggtg
      421 ccggtcacgt aattgagcac gccccgcgga ataccggctt cgtgcaacgc ttccaccaat
      481 cgataagaac tcagcggggc caaagatgcc ggctttaaaa cgacagcgtt gccggcaacg
      541 agtgcgggcg cgagtttcca ggcgggaatt gcgctgggaa agttccacgg cgtgatcaat
      601 gccacagttc cgagcggctt gcgaatcgtg tagcagataa cgttctcacg ctctgaagga
      661 acctggtaag taaactggcg cgagccttcg ccggcaaagt aacgaaagat gttgatggcg
      721 cgtttcactt cgccgactgc ttccggcagt gtcttgcctt cctcgcgcgt catctcctcg
      781 cccagttgcg gcagacgtgc ttcaaggagt tcggccgctt taaagagata ctctccacga
      841 cgcgttgcgg gcatttcggc ccatgctgtc tgtgccgcaa cagccgcagc gcacgcatcg
      901 tttacgtcgt cagcattcga cgcctgaaaa agaccgatcg tatcatcggt gttggcgggg
      961 ttgatgtttg gaaaagtctt accggattta ctctccgccc attcgccgtt gatgtagttt
     1021 ctgaacacct tcatctcgtt ctcctcgtag gacaggcatt cttgcctgtc tattaactat
     1081 aggccagaac gcctatcagg ttaggtgaca ggcaggaatg cctgtcctac ggtgcgattg
     1141 cactcttcgc ggtggaccac gacgcggtcg cttgcagcgc gtcaaaagcg cgatccagcg
     1201 gaaacggcgt gcttaccagt tgctcgaacg gaaattgatc gcgcgtgcgc gacagaaact
     1261 taagcgccgc ctgtgtgtgg cgtggctcaa agccccacga gccagccatg gtcagttgtt
     1321 ttctggtgac gatatgcgga ttaaacggaa tcgttccggc gtttccgtaa tggcccagaa
     1381 cgagatagcg tccgccatcg cgacaaagct ccactccttc cgtaacggcc tgcggaatgc
     1441 ctacacattc gagcaccaca tcagcgccaa agcctcccgt caattccatc acgcgcgcct
     1501 ttcgctcttc gactgaactc gcgtcgacgt caatactcac atcagcgcca aagcgtgtcg
     1561 ccagttgcaa tcgatgcggt ggaccaccaa agattatgat ctgcgcggcg ccgccggctt
     1621 tcgcgacggc taagcacgcg aggccaactg gtccggaacc ctgaatgaca acattgtcgc
     1681 ccattcgcac accgattctc tcgattccat gaagcgaggt gacgagcgca caacctgctc
     1741 caaccaccgc ttctgtcggc aattgttcgg ggattttgaa gatggccgtg ccgggccgga
     1801 ggtaaatgta atcggcgtaa ccgccgaata gatgcggcgc gacactgcaa tcgtaagaga
     1861 tgccataagc ctttcgtttc aaacagcgag tcggttcagg tttgacgcgg cagtaatagc
     1921 actcattaca gttgatggac gagctccacg tgatgcgatc gcctgctgcg atgggattat
     1981 tcgaccagtc gtgagtcagt ccctcgccaa cttcctcgat cacgccgaca gtttcgtggc
     2041 caagaatcac gggcaaggga atcggcaact gaccgtgcca gagatgcacg tcggtgccgc
     2101 agataccggc cagtttaact ttgaccagcg cggcgccagg ttcgatcgat tcggggatgg
     2161 gaaactcttt cagttcgagt ggagcttcgt agttagtaag tacagcggca cgagacgtct
     2221 tcaactgatc ctccggctcg ggcaatctat tctctcgggc aggctcgaag atataccaca
     2281 aaaagcggga accatacgaa gacgcggagt ctttaacccg ggaccgcggc cgtttgtcag
     2341 gtgtcgttgg agaaatcgta gaaaacgcgg aatagattgc cgtccagatc ggatactgtg
     2401 aactcatgta gcttccacgg cttcgactcc ggaggtgaaa tgatctttgc tccgctcgca
     2461 ttccaaatct cgtacagctc ttctacctct ttcttgctgt tgagattgag ccagatcagc
     2521 accggcgaag cattgccacg atgttcgcgg aagtcacgat ccgtaaggaa catccggcag
     2581 tgcccctttg agattccggc gatcccgccg gcgccgcccc aatctatgtt gaagccgagt
     2641 ttgtgctgat agtaatccag cgccgcgttc atgtcgctca caggtatctc aggtactgcg
     2701 gcgggaaatt caagcgaaat tttggtgatg tgtgtcacga gagaaccgtg tcctctgttg
     2761 ccagagtaac agaggacacg gacaacccac tactcacttc tctcgttcac tgctttatcc
     2821 aacgccacct cttggtccgt tggcgtgtcg cgctcgcggt cgccaaaagc ccccgcgact
     2881 ggattcgctg gcgggatcgg attgtaaccg tcgcgtgcgc gatcggtttt ttgtgcttcg
     2941 gaacgaacgc cgcttttctg tgattgttgc tctgacatga tcctcctcac ctcacgctgt
     3001 cggttgtacg ttcgctgtcg atgagccgcc gatgtgcaac tgtttcgaag cctggttagc
     3061 gaggtcgctg aaattggtat tggtgatcgc gcgtgttgtc agcagtagac ccgctgctcc
     3121 caaaccagcg ccgagcgcgc tgcgccgcat tccaccgtag gccgcgagtg atgcgccggc
     3181 ggcgccggcg agcagtctgg cggttggtga ccagttgtcc tggaacaact caaaccggct
     3241 gccctgacgt tcgcgtccgc cttgcagcgc cgggtgatta cccgcctctt cgtgaacttc
     3301 gagattgttg ttgatgtctt tgacgccgtg gatccatttc acgcacgaca gaagatgggg
     3361 gacttcctgc gcgagaattg gtccgtcaag cgttacgtgg ccgtcctggg ccgatacgtt
     3421 aattgccgca ggatgcgaaa cgattctgcc gatctttgcg cgcacgcaat caacgagcac
     3481 tgaatcgtcc ggttgttctg aagaaagtcg
//
LOCUS       DASTEY010000444         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_981776, whole genome shotgun sequence.
ACCESSION   DASTEY010000444 DASTEY010000000
VERSION     DASTEY010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981776"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..2143
                     /gene="asnB"
                     /locus_tag="VFO72_07820"
     CDS             212..2143
                     /gene="asnB"
                     /locus_tag="VFO72_07820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9479234.1"
                     /translation="MCGICGVWEYGANEGLITPSLIARMRDEMTHRGPDDSGDLIFDE
                     RRGGFGFRRLSIIDLSAAGHQPMHGCDERTWLVFNGEIYNHAILREELEARGHKYASR
                     TDSETILHLYEERGLDFIHHIEGDYAIALWDANKQQLVLVRDRIGVKPLYFYHKDGRF
                     IFASEIKAILQHPAVTPDIDEQSLYHYLTFLTTPAPTTLFRDIQKLPAGHMLTLKRDG
                     VLNIRQYWDALPPAQPAIRHESEHRAEILRLLRDSIRKRMMSDVPFGVFLSGGVDSSA
                     NVALMSELMSRPVETFTVGFSDAEYLNELESARRIANQFGTNHHEVIISEKEMQEFLP
                     RLVFHQDEPIADPVCVPLYYVSQLARDSGTIVVQVGEGSDEIFSGYDNYVRHLRIYES
                     FWRHAEILPRALRRALSSLSRPALEATGRKRAAIELIRRLGADEPLFWGGVVVYDETF
                     KPRVLSQSLRERINGLSSLRVVEPYLKQIEQERPGSDFLARMTYLELKLRLPELLLMR
                     VDKITMATSVEARVPFLDHHLVEYALGLPRSLKVEGITGKHILKRALENILPHDLLYE
                     AKRGFGAPVREWFRAERLGGWFDGHFLNSKLRQRDLLDYTFIARLLEEHRNQKHDWGF
                     HLWALLNLSLWYERWIDHS"
     gene            2125..>2806
                     /locus_tag="VFO72_07825"
     CDS             2125..>2806
                     /locus_tag="VFO72_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479235.1"
                     /translation="MDRPFVIERKFSKDVAWTLATRLLMIVNSVTAGIIVARWLGARG
                     VGELAVINVAVATIVQLGSFGLPSSNTYFIAQDRAHFRAATINSLLFATVTGSVLALA
                     LSGVASFRPDWFGFVPPELIRIASVQIPFLLITLIGLNILLALGKLREFNLLDLAGQS
                     FVLVNALLILIVFGLGLDALVKLNTIASVLVSLVVVVLLVGGGKKLVDSEWRADVALL
                     RRMIVYGLK"
BASE COUNT          557 a          757 c          815 g          677 t
ORIGIN      
        1 tttccagtag agatgcagtt tgaaatgctg aaaacgttta cctgggacgc cgtttacctg
       61 ggaccgcagg cgtctcgcct gcaagggaac gagtcgggcg ctaccgtgag atgcaggcga
      121 gacgcctgcg gtcccaggta aacggcgtcc caggtaaacg ttttcagcat ttcaaactgc
      181 atctctactg gaaaatttat ccgagtaagt aatgtgcgga atctgcggag tttgggaata
      241 cggtgcaaac gaaggcttga ttacgccttc gctcatcgcg cgaatgcgcg atgagatgac
      301 gcaccgcggt cctgacgact cgggcgactt gatcttcgac gagcgtcgcg gcggtttcgg
      361 gtttcgccgg ttgtcgatca tcgatctctc tgctgccggt catcagccga tgcacggctg
      421 cgatgaacgc acgtggctgg ttttcaacgg cgagatttac aaccatgcca tcctccgtga
      481 agaactcgaa gcgcgcggtc acaagtacgc ctcacgcact gactccgaaa ccattctgca
      541 tctgtacgaa gaacgcggcc tcgacttcat ccaccatatc gaaggcgatt acgccatcgc
      601 gttgtgggac gcgaacaagc aacaactcgt gctcgtccgc gatcgcatcg gcgtcaaacc
      661 gctctacttc tatcacaaag acgggcgctt catcttcgcg tctgagatca aagcgatact
      721 gcaacatccg gctgtcacac cggacatcga cgagcaatcg ctctaccact atctcacttt
      781 cctgacgacg cctgcgccaa ccacgttgtt tcgcgacatt caaaaactcc cggcgggcca
      841 catgctcaca ctcaaacgcg acggcgtgct gaacatccgc cagtattggg acgcgttacc
      901 tccggcgcag ccggcgattc gccacgaaag cgaacatcga gcggaaatac tgcgcctgct
      961 ccgtgattcg attcgcaaac gcatgatgtc cgacgttcct tttggcgtgt ttctgtcggg
     1021 tggtgttgat tcgtcggcga acgtggcgtt gatgtcggag ctgatgtcgc ggccggttga
     1081 aaccttcacg gtcggtttca gcgatgcaga gtatctgaac gagctcgaat ccgcgcgtcg
     1141 catcgcgaac cagtttggca cgaaccatca cgaagtcatt atctcggaaa aggagatgca
     1201 ggagtttctg ccgcgactcg tttttcatca ggatgagcca atcgcggatc cggtatgcgt
     1261 gccgctttac tacgtttcgc agttggcgcg cgattcgggc acgatcgtgg tccaggtggg
     1321 cgagggatct gacgaaatct tcagcggtta cgacaactat gtgagacatc ttcggattta
     1381 cgagagtttt tggcggcacg cggagatttt gccgcgtgcg ttgcggcgcg cgttgtcgag
     1441 tttgtcgcgc ccggcgctcg aggccaccgg aagaaagcgg gcggcgatcg aattgattcg
     1501 ccgtcttggc gccgacgagc cgctgttttg gggcggcgtg gttgtttacg atgagacgtt
     1561 caagcctcgc gtgttgtcgc agtcgctgcg tgaacggata aacggtcttt cgtcgttaag
     1621 agttgtcgaa ccatatctga aacagatcga gcaagagcgt cccggttcgg atttcctggc
     1681 gcgcatgacg tatctggaat tgaaactgcg tttgcccgag cttctgctca tgcgtgtcga
     1741 caagatcaca atggcaacga gcgtggaagc gcgagtgccg tttctggatc atcatctggt
     1801 tgagtacgcg ctcggtctgc cgcgctcgtt gaaagtcgaa ggaataaccg gcaaacacat
     1861 cctcaagcgc gcgctggaga acattctgcc gcacgatctg ctttacgaag cgaagcgcgg
     1921 ttttggtgcg ccggtgcgcg agtggtttcg agcggagaga ttaggcggat ggtttgacgg
     1981 tcatttcctg aactcgaagc ttcgtcagcg agatctcctc gactacacgt ttatcgcgcg
     2041 gctgctggaa gaacaccgaa accaaaagca tgattggggt tttcaccttt gggccttgtt
     2101 gaatctcagt ttgtggtatg agaggtggat cgaccattcg tgattgagcg taagttctcg
     2161 aaggacgttg cgtggacgct tgctacgcga ctgctgatga tcgttaacag cgtcacagcc
     2221 ggaatcattg tcgcgcgttg gttgggagcc aggggcgtcg gagagttggc ggtcatcaac
     2281 gttgcggtag ccaccatcgt tcagcttggc agttttggtc tgccgtcttc gaacacctac
     2341 ttcattgcgc aagaccgcgc tcatttccgc gccgcgacaa tcaactcttt actcttcgcc
     2401 acagtaaccg gttccgtcct ggcgcttgcg ctgagcggcg tcgcgtcttt tcgtcccgat
     2461 tggttcggct tcgttccccc ggaattgatc cgcatcgcat ccgttcaaat ccctttcctg
     2521 ttaatcacgc tcatcggcct caacattctt ctcgcgctgg gaaagctacg agagttcaac
     2581 ctgcttgacc ttgcgggtca gtcgtttgtt ctggtcaacg cactcttgat tctgatcgtt
     2641 tttggcctgg gtctcgatgc gctggtgaag ttgaacacga tagcgagcgt tttggtcagt
     2701 ctcgtggttg tcgtgctgct cgtgggcggt ggaaagaaac tcgtcgactc cgaatggcgt
     2761 gcggacgtgg cgctgctgcg gcgcatgatt gtttacggac tcaagt
//
LOCUS       DASTEY010000445         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1459767, whole genome shotgun sequence.
ACCESSION   DASTEY010000445 DASTEY010000000
VERSION     DASTEY010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459767"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VFO72_07830"
     CDS             <1..1460
                     /locus_tag="VFO72_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479236.1"
                     /translation="LPIADCQIFILLTRQSTIGNQQSAIDNAMNKPATSHSSLDPLLG
                     RRGRLRFERHVTILALAAGSPGVLLCAFLLWYGNYSSQVQWTVDLLLVLLWLGISFNL
                     KQRIVRPLQTLSNILAAIREGDYSLRGRRALSGDALGEVMLEVNDLGQTLREQRLGAM
                     EATALLRTVMAEIDVAVFAFDGEHRLQLVNRAGEKLLAQPSARLLGRTSEELGLSECL
                     NRPAGAGPQTMQMVFPGGVGRWDIRRSTFREGGAQHQLLVLTDLSQTLREEERTAWQR
                     LLRVLGHELNNSLAPIKSVAGSLADLMRRDPEPADWREDMHRGLAVISSRADSLARFV
                     ESYSKLARLPPPRLEPLKIGTLVSRVASLETRLPVNVVSGPDVMVRGDDVQLEQLLIN
                     LVRNAVDASMETHGSVEVGWQQNNGNVEVWIRDEGPGLASTANLFVPFFTTKAEGSGI
                     GLVLSRQIAEAHGGTLTLENRRQFRGCEARLRLPI"
     gene            complement(1535..>2330)
                     /locus_tag="VFO72_07835"
     CDS             complement(1535..>2330)
                     /locus_tag="VFO72_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9479237.1"
                     /translation="AKASDVALLVLGGNEDTNKEGWAENHLGDRDSLELFGRQNDLVR
                     AVLATGKPVIVMLINSGPLSINYIAENVPAILEGFYLGQETGVAVADVLFGDYNPAGK
                     LPISFPRSVGQLPIYYNQKPSARRGYLFADKSPLFPFGYGLSYTSFSYGKPGVAPAQI
                     PAEGQAKVTVAVTNTGKRPGDEIVQLYIRDLVSSVTRPIKELKDFKRISLQPGETKAV
                     EFVITPDKLWFYDLNMKRVVEPGTFEIMVGPSSVQHQSVKLEVGHR"
BASE COUNT          440 a          634 c          712 g          544 t
ORIGIN      
        1 gattgccaat tgccgattgc caaatcttta ttcttttgac tcggcaatcg acaatcggca
       61 atcaacaatc ggcaatcgac aatgcgatga ataagcctgc gacatctcat tcctcactag
      121 atccgctact cgggcgccgt ggccggctga ggttcgaaag gcacgtcacg attctagccc
      181 tcgctgccgg ttctcccggc gtcttactgt gtgcctttct gctctggtat ggcaactact
      241 cctcgcaggt gcagtggact gtcgatctgc tgctcgttct tttgtggctc ggcatatcgt
      301 tcaatctcaa gcaacgcatc gtccggccgc tgcaaactct ctcaaacatt cttgccgcca
      361 ttcgcgaagg tgattattcc cttcgcggcc ggcgcgcgct ctcgggtgac gcgctcggcg
      421 aggtgatgct ggaggttaac gatctcggcc aaacgttgcg cgagcaacgg ctcggcgcga
      481 tggaagccac cgcgctattg cgcacggtga tggcggagat cgacgtcgcc gtgtttgcat
      541 tcgatggcga acatcgcttg cagctcgtga atcgtgcggg cgaaaagctt ttggcccaac
      601 catcggcgcg tctcctgggt cgaacgagtg aagagcttgg actttccgag tgtttgaatc
      661 gtccggcggg cgccggacca caaacgatgc agatggtttt tcccggtggc gtgggacgtt
      721 gggacattcg tcgcagcact tttcgcgaag gcggcgcgca acaccagttg ctcgtgctga
      781 ccgatcttag tcaaacattg cgcgaagaag agcggactgc gtggcaacgg ttgttgcgtg
      841 ttttgggtca cgaactaaac aactcgctcg cgccgatcaa gtctgtcgcg gggagtctgg
      901 cggatctgat gcgacgcgat cccgaaccgg ccgattggcg tgaagacatg catcgcggtt
      961 tggcggtgat ctcctcgcgc gcggattcac tggcgcgttt tgtggagtcg tattcgaagc
     1021 tcgcgagatt accaccgccg cggctcgaac cgttgaagat cggaacactc gtgtcgcgtg
     1081 tggcgagtct cgagacgcgt ttgccggtga acgtcgtttc cggtccggac gtgatggtgc
     1141 gtggagacga cgtgcagttg gagcagttgc tgatcaatct cgtgcgcaac gcggtcgatg
     1201 cgtcgatgga gacgcatggt tcggtcgagg ttggctggca gcagaacaac gggaatgtcg
     1261 aggtgtggat tcgcgacgaa gggccgggac tggcgagcac ggctaatctg tttgtgccgt
     1321 tctttacgac gaaggcggaa ggatcgggaa ttggattggt gttgtcgcgt cagattgcgg
     1381 aagcgcatgg cggcacgttg acgctggaga accgccggca gtttcgtggt tgcgaggcgc
     1441 ggctacgact gcctatctga tccctaacag aagatcgatc gtcaactggt cgagccgttc
     1501 gtaaggcagg gtactgtgtc tgtgtgctcg gtgtttatct gtggcccact tcaagcttta
     1561 ccgattggtg ctggacggaa ctcggtccga ccataatttc gaacgtgccc ggttccacga
     1621 cgcgcttcat gttcaaatcg tagaaccaaa gcttgtcggg cgtaatcaca aactccaccg
     1681 cctttgtttc gccgggttgc agcgagatgc gtttgaagtc tttcagttcc tttatcgggc
     1741 gcgtaacgga actcaccagg tcgcgaatgt agagttgcac gatctcatcg ccggggcgct
     1801 tgccggtgtt tgttaccgcg accgtgactt tcgcctgacc ttcggccggg atctgcgcgg
     1861 gcgcaacgcc tggtttgccg taggaaaagc tcgtgtaact caagccatag ccgaagggaa
     1921 acaacggtga cttgtcggca aacagatagc cgcgacgtgc gctcggtttc tggttgtaat
     1981 agattggcag ttgcccgacc gaacgcggaa aactgatcgg cagcttgccc gccgggttgt
     2041 aatccccaaa caacacgtcc gctactgcca cgcccgtttc ctgtccgaga tagaagcctt
     2101 cgagaatcgc gggcacgttc tctgcgatgt agttgatgga aagtggacca ctgttgatga
     2161 gcatcacgat taccggcttg ccggttgcga gcaccgcgcg aaccaggtcg ttctgcctgc
     2221 caaacagctc cagactgtcg cgatcgccca ggtggttctc ggcccagcct tctttgtttg
     2281 tatcttcgtt gccgcctaac acgagtaatg cgacgtcgga ggctttcgca
//
LOCUS       DASTEY010000446         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_817313, whole genome shotgun sequence.
ACCESSION   DASTEY010000446 DASTEY010000000
VERSION     DASTEY010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817313"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VFO72_07840"
     CDS             complement(<1..1262)
                     /locus_tag="VFO72_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9479238.1"
                     /translation="MTQTPPLTKSHGHLLRVLGVGFGLAVIIGNTIGAGIFRAPGQIA
                     EQLPHPWLFLGVWLLGGLYALLGAISLAELGTMIPRSGGQYVFARYALGEYAGFIVGW
                     SDWISSCGSTAAVALVVGTFSGALFPALSGKAPAIAAAVAIVFAVLQWRGIVWGSTIQ
                     NLTSLLKALVFFALIAAAFILGGDGALTSSQTYSTSAVTMAAVVASLQAAIYTYDGWS
                     GVIYFSEEVQHPGRDIPRALLGGVLTITAIYLLVNVALLYVLPISGIAGKEFAAGEAA
                     NVIFGRYGDAVFRSVTIVSMLSSINALHLMATRVIFAMSRDGLFSAKAAIVNVGGTPT
                     VSLFLSASVAVLFIFFGQTFDRVITVLAFFFVANYILSFISLFVLRFREPEKPRPYRA
                     WGYPVTPGLALVGSILFLTGAVYADRTN"
     gene            complement(1259..>2299)
                     /locus_tag="VFO72_07845"
     CDS             complement(1259..>2299)
                     /locus_tag="VFO72_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9479239.1"
                     /translation="SWYVNKELYEAELKTIFSNTWPLAGRLDQVSQPGRYLTTDIAGE
                     PIVVVRGNDGVLRAFFNVCRHHAAAVMTEPEGKAPQLRCPYHGWTYSLEGELKGTPDF
                     AGVCEFDRTRNGLAPVEIAEWENWIFIKLDHTNSVSLMDFLTTDLTDQINPLELSHLH
                     WFERRHYSFDCNWKVFVDNYLDGGYHVPYLHKGLDSVLDYSNYMIENGERHCLQWSPI
                     VTDGAEAQTGAVRKGDRALYYWIYPNFMINWYDGVMDTNLVVPRGVDQTEVIFDFYFP
                     DVVSEEARARNAASVAVGQRIQGEDVAICKSVQCGLNSRAYTAGRLSVRREAGEHLFH
                     RLLHADLSRANL"
BASE COUNT          562 a          685 c          630 g          422 t
ORIGIN      
        1 ctgttggttc gatcggcgta caccgctccc gtaagaaaca gtatcgatcc aaccaacgcc
       61 aaacccggcg tcactggata accccacgcg cgataaggtc gcggcttttc gggttcgcga
      121 aaccgcagca caaacagaga aatgaacgag aggatgtaat tcgccacgaa gaagaacgcg
      181 agtacggtga taacgcgatc gaaagtctgg ccaaaaaaga taaagaggac cgcgaccgac
      241 gcgctcaaaa acagcgacac cgtcggcgtt ccccctacgt tgacgatcgc tgcctttgca
      301 ctgaacagcc catcgcgact catcgcaaaa atcacacggg tagccatgag atgaagcgca
      361 ttgatgctac tcagcattga gacaatcgtc acagaccgga acaccgcgtc gccataccgt
      421 ccaaagatca catttgccgc ttcgcccgcc gcaaactcct tgcctgcaat tcccgagatc
      481 ggcaacacgt aaagcagcgc gacgtttacg agcagataga tcgcggtgat cgtcagcacg
      541 ccgccaagca atgcccgcgg aatgtcgcga ccgggatgct gcacttcttc ggaaaaatag
      601 ataacgccgc tccagccatc gtaggtgtag atagccgcct ggagtgacgc gacaaccgcc
      661 gccatcgtca cggcggaagt tgaatacgtt tgcgaactgg ttagcgcccc atcgccgccg
      721 agaatgaagg cagcggcaat caaggcgaag aaaacaagcg ctttgagcag actggtgagg
      781 ttttgaatcg tgctgcccca aacaatgccg cgccactgta agaccgcgaa gacaatcgcg
      841 actgccgcgg caatcgccgg cgcttttcca ctcaacgcgg gaaagagcgc gcccgagaat
      901 gtcccgacga ccagcgccac cgccgcggtt gagccacacg acgagatcca gtcgctccaa
      961 ccgacgatga acccggcgta ctcgccgagc gcgtagcgag cgaagacgta ctggcctccc
     1021 gaacgcggaa tcatcgtgcc gagttccgcg agcgaaatcg cgcccagcag agcgtataga
     1081 ccacccaaca accacacgcc gagaaacagc cacggatgcg gaagttgttc ggcgatctgt
     1141 ccgggcgcgc gaaaaattcc ggcgccaatc gtgttgccga tgatcaccgc cagtccaaac
     1201 ccaaccccga gcacacgcaa caaatgccca tgagatttgg ttagcggcgg agtttgcgtc
     1261 acagatttgc gcggctcaag tcggcgtgca gaagtcgatg aaagagatgt tcgccggctt
     1321 cgcgtctaac ggagagccgg ccggcggtgt aggcgcgcga gttcagtccg cattgcactg
     1381 acttacagat cgcgacgtcc tcgccctgaa tacgttggcc tacagcgacg ctggctgcgt
     1441 tgcgggcacg ggcctcctcg ctgacgacgt cgggaaaata gaagtcgaag attacttccg
     1501 tttggtccac accgcgcggc acgacgaggt tcgtatccat cacaccgtcg taccagttga
     1561 tcataaaatt tggatagatc cagtagtaaa gcgcgcgatc ccctttacgc acagcgccgg
     1621 tctgtgcttc ggcgccgtca gtcacaatcg ggctccactg caaacagtgg cgctcgccgt
     1681 tctcgatcat gtaattgctg tagtcgagca cgctgtcgag acctttgtgc aggtaaggca
     1741 cgtgataacc accgtcgaga taattgtcga caaagacctt ccagttgcag tcgaaactgt
     1801 agtggcgacg ctcaaaccag tggagatgcg acagctcaag cggattgatc tggtcagtca
     1861 aatccgtggt taaaaaatcc atcaaagaaa cgctgttggt gtgatccagt ttgatgaaga
     1921 tccagttttc ccactctgca atctcaacgg gcgctagccc gttgcgcgtc cgatcaaact
     1981 cgcacactcc agcgaaatcc ggcgtgcctt tcaactcgcc ttccagtgaa taggtccagc
     2041 cgtgatatgg acaacgcagt tgcggcgcct tgccctcggg ttcggtcatc acagccgcgg
     2101 catgatgccg gcaaacgttg aagaaagcgc gcaaaacgcc gtcgtttcca cgcacgacga
     2161 cgatcggctc gcccgctatg tcggtggtca ggtaacgtcc gggctgactc acctggtcca
     2221 aacgtccggc gagtggccat gtgttggaga agatggtttt taactcggct tcgtaaagtt
     2281 ccttgttcac gtaccagga
//
LOCUS       DASTEY010000447         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1130504, whole genome shotgun sequence.
ACCESSION   DASTEY010000447 DASTEY010000000
VERSION     DASTEY010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130504"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..735)
                     /locus_tag="VFO72_07850"
     CDS             complement(154..735)
                     /locus_tag="VFO72_07850"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9479240.1"
                     /translation="MCVICHTDTKTSNPPLRNFPARFNEPFNVKFDHAQHMTGAARPQ
                     NGCVGCHNSPVHRRAGLSIPANLAAHNNCYTCHTPTSRSNAGREIASCGVCHDQKSYR
                     RTSTNARAYARAFSHANHGTRQRLACADCHKVTAGAAQTRQVSSTTAAQHFAAGRAQS
                     CATCHNGRRTFGGDLGFRDCRRCHSGPTFRMPM"
     gene            complement(954..1928)
                     /locus_tag="VFO72_07855"
     CDS             complement(954..1928)
                     /locus_tag="VFO72_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479241.1"
                     /translation="MNTILKACVFICVCLWPIHGAVDYTKFSHSTPKHKSACNTCHKP
                     PTKNWTKVSSFPDVADFPDHVACVSCHRAQFFRGTRPPICSVCHSKTSPRDDVRYAFR
                     NPASRLQFTIEFPHDRHQDVIAQRAQNPWRFRVLAHAAPQNFNNCTLCHAQATVVSKP
                     PSGGWIDSYVPPLATFKASPVDHSSCFNCHWKSQEPVATNCNGCHKLATSDVAANAPT
                     RISLKFIHDGGGEKKQHVAECTTCHINITKSATLRGLKPDVPITSCTECHNKEGLRQD
                     LNKELIALDKNRAFTCVYCHTSNIGSLDAPASHYLIAQRPPLKRRDIK"
     gene            complement(1925..2716)
                     /locus_tag="VFO72_07860"
     CDS             complement(1925..2716)
                     /locus_tag="VFO72_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HET9479242.1"
                     /translation="MKGATRLLFALIALVLALPLTVGHSRNQDTAARQSGVLRLRARV
                     KLNDSAPLRGLPRKRFFLIRGSLEQNRALVDAIERQQLVTRDCYYSKKGASPALIEWL
                     KEADCESVYCREIEQGFVSGPKAVPEFATAFAASEKEFGNGSTALKWLAMNLPVDLRD
                     GFYRDRKGALDALLKQAQTSSGAPVQSVMTDRNGTAYFTDLVPGTYVISNLIPAELGQ
                     TLATWNCEVQIKNDDLASEKPYLISNRKERLVKCVAAEKPVPACP"
     gene            complement(2721..>3300)
                     /locus_tag="VFO72_07865"
     CDS             complement(2721..>3300)
                     /locus_tag="VFO72_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET9479243.1"
                     /translation="DAPVFGFDVAVQKARTAAFFSNANAATALRAAGLGAYVDRAIAD
                     GVRLDGAIAFSDRATGFLHRPFFPDGIDNTAAGPFSTAIGSWSPFNVGLQLDLVRTNF
                     LAALSGPVTSCTSIPALPNGIQTFAGSVPLYKNGVLVGAIGISGDGIDQDDLIAAAGG
                     NGFSPPVDIRSDQFFVRGVRLPFLKFPRSPNL"
BASE COUNT          704 a          869 c         1000 g          727 t
ORIGIN      
        1 tgaaagggac ttcgccgtac ttatccgatt ggctgtcctt gctcaacagg acttctttgg
       61 gctggacctt gctcgctctt tcctgaacaa caagaccgaa acagccaagc gctagaagaa
      121 gaatcacgag ggttttcata ggcgcgaatc ttatcacatg ggcatgcgga aggtgggacc
      181 agaatgacaa cgcctacagt ctctgaatcc aagatcgccg ccaaacgttc tcctgccgtt
      241 gtggcaggtc gcgcaacttt gcgcgcggcc agctgcgaag tgttgcgcgg cagttgtcga
      301 actcacctgt cgcgtctgcg ctgcgcccgc cgtgactttg tggcagtcgg cgcatgcgag
      361 tcgctgccgc gtgccgtggt tcgcgtgact gaaggcgcga gcgtaagcgc gcgcgttggt
      421 gctggttcga cgatacgatt tttgatcgtg acagacgccg cacgatgcga tctcgcgacc
      481 cgcgtttgaa cgacttgtcg gcgtgtgaca ggtgtaacag ttgttgtgcg ccgcgaggtt
      541 tgcggggatc gacaacccgg cgcgccggtg cacgggcgag ttgtgacagc ccacacagcc
      601 attctgcgga cgcgccgcgc cggtcatgtg ttgcgcgtga tcgaacttca cgttgaacgg
      661 ttcgttaaag cgtgcgggaa aattcctcaa cggcggattg ctggtcttcg tatctgtatg
      721 acaaattacg cacataggta tcgcgggtgt cgtgaactgc ccgcggtggc aatcggtaca
      781 tgcccggtga ccggggaaac gaggcgtcgc agaattgtcg gtgcgttcgt gacaagagtt
      841 gcaggcaagc gaacggtgcc gctcgctgct gtggagaaac ttcgagtaat cgacttcggg
      901 gccctgggcc acgaatgaac agagaagaca cagattaaga atggccagtg cgatcatttt
      961 atatctctgc gcttcagtgg tggacgttgt gcgatcaggt agtggcttgc gggtgcatca
     1021 agactaccga tgtttgacgt gtggcagtag acgcaggtga acgcgcgatt cttgtcgagt
     1081 gcgattagtt ccttgttgag atcctgtcgg agtccttcct tgttgtgaca ttcagtgcag
     1141 gaagtgatcg gcacgtcagg tttgagacca cgcaacgtgg ctgatttagt gatgttgatg
     1201 tggcatgtcg tgcattcggc gacgtgttgc ttcttttcgc cgccgccgtc gtgtatgaac
     1261 ttgagcgaaa ttcgtgttgg cgcgttggca gcgacgtcgg aagttgcgag tttgtgacag
     1321 ccgttgcagt tggtggcgac aggctcctgc gatttccagt gacagttaaa acaggaagaa
     1381 tgatcgacgg gcgacgcttt gaacgtggca agcggcggca cgtacgagtc aatccatcca
     1441 cctgaaggtg gcttggaaac aactgttgcc tgcgcgtggc agagagtgca gttgttgaag
     1501 ttttgcggag cggcatgggc cagcactcgg aaccgccatg gattttgcgc tcgctgagct
     1561 atcacgtcct gatgccgatc gtgaggaaat tcgatcgtga actgtaatct cgaggcgggg
     1621 ttacggaatg cgtagcgtac gtcgtcgcgc ggagatgtct ttgaatgaca aacggaacag
     1681 atcggcggac gcgtgccgcg gaagaattgt gcgcgatgac aactcacgca ggctacgtga
     1741 tccgggaagt cagcgacgtc cggaaaggat gaaactttgg tccagttttt agtcggtggt
     1801 ttgtggcacg tgttgcaggc tgatttgtgt ttgggtgttg aatgagagaa cttggtgtaa
     1861 tcgaccgcgc catgaattgg ccacagacag acacagatga acacgcatgc tttaaggatt
     1921 gtgttcatgg gcacgcggga actggttttt cggcggcaac acacttgacg agccgctcct
     1981 tgcggtttga gatgagatac ggtttctcac tggctagatc gtcgttcttg atctgcactt
     2041 cgcagttcca ggtcgcgagt gtttggccca gttcagccgg aattagattc gatatgacgt
     2101 acgtgcccgg aacgagatcg gtgaaatacg cggtgccgtt ccgatcggtc atcacggact
     2161 gaaccggcgc gcctgaactc gtttgcgcct gtttgaggag cgcatcgagc gcgcctttgc
     2221 gatctcgata aaaaccgtcg cgcaaatcca ctggtaaatt catcgccaac cacttcagcg
     2281 ccgtcgatcc attcccaaac tctttctcgc tcgcggcaaa cgccgtcgca aattcaggca
     2341 ccgcctttgg accactgaca aagccctgct cgatttcgcg gcagtacacc gactcgcagt
     2401 cagcctcctt cagccattca atcaacgcgg ggctcgcgcc ttttttggag taataacagt
     2461 cgcgcgtgac cagttgctgg cgctcgatcg cgtcgacaag cgcgcggttt tgttcgagcg
     2521 aaccgcgaat gagaaagaag cgtttgcgag ggagaccacg cagcggcgcc gaatcgttga
     2581 gttttacgcg agcacgcaac cgcaacacgc cggattgacg tgcggccgtg tcctgattgc
     2641 gtgaatgccc aacggtcagc gggagtgcga gcaccagcgc gatgagagcg aacagtaagc
     2701 gagtggcgcc tttcatctgt ttagagattc ggactccgcg gaaacttgag aaacggcaag
     2761 cgtacgccgc gaacgaaaaa ctgatccgaa cgaatatcta ccggcggtga aaaaccattc
     2821 cccccagccg ctgcaatcaa atcgtcctga tcgataccgt cgccactgat cccgatcgcg
     2881 cccaccagca cgccgttttt gtagagcggc acgctgccgg caaaagtttg aatgccattt
     2941 ggcaacgccg gaatggaagt gcatgaagtg actggtccac ttagcgccgc cagaaagttg
     3001 gtgcgcacaa gatccagttg tagtccaacg ttgaaggggc tccagcttcc gatggccgtg
     3061 ctgaatggac cagcggcagt gttgtcaatt ccatccggaa agaacggtcg gtgcagaaat
     3121 ccagtcgcac gatcgctaaa cgcgatggcg ccgtcgagcc gcacaccgtc cgcaatcgcg
     3181 cgatccacgt aggctccaag accggctgct cgcaacgccg tcgccgcgtt tgcattcgaa
     3241 aagaaagcag cagttctcgc cttttgcacg gcgacgtcaa acccaaacac cggcgcatcc
//
LOCUS       DASTEY010000448         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1212892, whole genome shotgun sequence.
ACCESSION   DASTEY010000448 DASTEY010000000
VERSION     DASTEY010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212892"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="VFO72_07870"
     CDS             complement(<1..1360)
                     /locus_tag="VFO72_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479244.1"
                     /translation="MDSVFKDLQFAFRSLFKRPGFMAVAIVTLALGIGGSTAIFTVVD
                     AALLRGLPYKSPDRLYHMWEKTPQGEFSKREFSYPDYQDYQQNNVFEGLAAYTGGRVL
                     LSGDGEPESVGGPRVSANFFNVLGVDPILGRTFQAGEDQQGGPRVTVLTYGLWQRRFG
                     GDPAVIGRTLTINGESYTIIGVLPVSFQFALRTADLWTPYQPTQNQLTRRFLHGTNLI
                     GRLKSGVDEAQAQSDLSVIGARIEREHNASHAGTSVRLVPLQEEVVGRVRPILLVLLG
                     AVGFVLLIACANVASLLLTRSLARQKEVAIRSALGASRWRVVRQLLTESIVLSLLGGA
                     AGLLIAFWGVPALVSALPQNQLNAMPFLSTLSLDAGILAFSFALSLGTGLVFGLAPAL
                     QSSRLDLNEVLKEGGRNTAAGAGHRLRSAMVVTEIALAVVLLVGAGLMMKSLLRLLQT
                     NIG"
     gene            complement(1360..>2399)
                     /locus_tag="VFO72_07875"
     CDS             complement(1360..>2399)
                     /locus_tag="VFO72_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9479245.1"
                     /translation="FYNELIEKLKTLPGVEAAGATTQTPLSPGDNWSAFTIEGRPDPP
                     PGQQQQAAMRIVSSDYFTTMKIPLRKGRFFSNADARAALPLIRWFDQQPYPERFNEPQ
                     PAPAAIINETMARLYWSNEDPLGRRLKIIASPWITVVGVVGDVRHSGLNTPANPEIYL
                     SDLQEPQSALAVMVRTTGDPLQLAAMAREQIKAIDKDQPVVISTMDQILSASVAGRRF
                     NTLLLGIFATVALLLAMVGVFGVIHYSVVQRTHEIGIRIALGAQRHDVFKLVVGEGLA
                     LALIGVALGSAGAWAMTRLISGLLYGVSPTDGPTFILVSVVVTLVALLACYLPARRAT
                     KIDPLIALRYE"
BASE COUNT          542 a          728 c          667 g          462 t
ORIGIN      
        1 acccgatgtt tgtttgcagt aaccgcagta gactcttcat catcagcccg gcgcccacga
       61 ggagcacaac cgcaagcgcg atctcagtga cgaccatcgc gctgcgcaag cgatgtcctg
      121 cgcccgctgc tgtattgcga ccgccctctt tcaacacttc gttcagatcc aaacgtgacg
      181 actgcaacgc cggcgctaag ccaaagacaa gacctgtccc aagcgagaga gcaaaggaga
      241 acgcgagaat gccggcgtca agactcaacg tgctgagaaa cggcatcgcg ttcaactggt
      301 tctgcggcaa tgccgaaacc agcgcaggca cgccccaaaa agcgatcagc aacccggcag
      361 ccccgccgag tagtgaaagt acgatcgact cagtcaacag ttgccgcacc acgcgccacc
      421 ggctcgcacc cagcgcggaa cggatcgcga cctctttctg ccgcgccagc gaacgcgtca
      481 acaaaagact cgcgacgttc gcacacgcga tcagcagtac aaacccgact gcccctaaca
      541 atacgagcag tatcggtctg actcttccga ccacctcttc ctgcagcgga acaagtcgga
      601 cagacgttcc ggcgtgtgac gcgttgtgtt cgcgctcgat gcgagcgcct atcacgctca
      661 agtcggattg cgcctgcgcc tcatcgacgc ccgatttcaa ccggccgatg aggttcgtcc
      721 cgtgcaggaa ccggcgcgtc aactggttct gcgtgggttg atacggtgtc caaaggtctg
      781 ctgttctcag tgcgaattga aaactcacgg gcaaaacgcc gatgatcgtg tagctctcgc
      841 cgttgattgt cagcgttcgt ccgataactg cgggatcgcc gccgaaccgt cgttgccaca
      901 agccgtacgt aagaaccgtt actcgtggtc caccctgctg atcctcgcct gcttgaaacg
      961 tgcggcccag aatcggatcg acgccgagca cgttgaagaa attcgcactc acgcgcggcc
     1021 cgccgacact ttcgggttca ccgtcgccgc tcagcagcac gcgaccgccg gtgtatgcag
     1081 cgagtccctc gaagacgttg ttctgctggt aatcctggta atcgggatag gagaactctc
     1141 gttttgagaa ctcgccttgc ggggtctttt cccacatgtg atagagacga tcgggtgact
     1201 tgtaaggcaa gccgcgcaac aacgcggcat cgacaacggt aaagatggca gtgctgccac
     1261 cgataccgag agcaagcgtc acaatggcaa ccgccatgaa accggggcgc ttgaataaac
     1321 tgcgaaacgc aaattgaaga tctttgaata cagagtccat tactcgtacc tcaatgcaat
     1381 taatggatcg attttcgtcg cgcggcgcgc cggtaagtaa cacgccagaa gcgcgaccaa
     1441 cgtcaccacg acggaaacca aaatgaaggt tggaccatcg gtcgggctca cgccgtacag
     1501 cagtccactt atcaggcgcg tcatagccca ggccccggca gagcccagcg ccactccaat
     1561 taacgccagc gccagtccct caccgacaac gagcttaaag acatcgtgcc gctgggcgcc
     1621 gagcgcgatc cgaatcccga tctcatgtgt gcgctggacc accgaatagt ggatgactcc
     1681 aaacacgccg accatggcca gaagcagcgc aacggttgca aaaattccga gcagaagagt
     1741 attgaatcgc cgaccggcga ctgaggcaga taggatctgg tccatcgtgg agataacgac
     1801 tggctggtcc ttatcgattg ctttgatttg ttcgcgcgcc atggcagcca gttgtaatgg
     1861 atcgccggtg gtccgaacca tcacggccag cgcagattgc ggttcttgca ggtcagacaa
     1921 atagatctcg gggttagccg gtgtatttag accgctgtgg cgtacgtctc cgacgactcc
     1981 gacaacagtt atccagggac tggcaatgat cttcagacgg cggcccagtg gatcttcgtt
     2041 ggaccagtaa agccgcgcca tcgtctcgtt gatgattgct gctggagccg gctgcggctc
     2101 gttgaagcgt tcgggatatg gctgctgatc gaaccagcga atcagaggca gcgccgctcg
     2161 ggcatcagcg ttactgaaaa aacggccctt ccgcagcgga atcttcatcg tggtgaagta
     2221 gtcgctactc acgattcgca tggccgcctg ctgctgttgc cctggcggtg gatccggacg
     2281 gccctcgatc gtaaatgcgc tccagttatc accggggctg agtggcgtct gtgtggtcgc
     2341 gccggccgct tcaacccccg gcaacgtctt tagcttttca atcagctcgt tatagaagg
//
LOCUS       DASTEY010000449         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_421962, whole genome shotgun sequence.
ACCESSION   DASTEY010000449 DASTEY010000000
VERSION     DASTEY010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421962"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2005)
                     /locus_tag="VFO72_07880"
     CDS             complement(<1..2005)
                     /locus_tag="VFO72_07880"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9479246.1"
                     /translation="MSQAGTKTQSEVGVFTTDADLIIRVWDATLARLTGIPDETAAGR
                     ALTALLPDLEKRALLKRFQRALRKGVVEVLAPAFHHYLIPCAPVSPSKYFDKMQQRVT
                     IAPLRDSNTIAGLIVTVEDVTERLERERELATHLASGDEAMRLEAAESFSVDEFYDAA
                     PLLDALSDESWRVRRAAVKGMSQRAAPEAIAALLNSVVENHHNPSLLNSALQILSSSD
                     VDTLSPLLELLQGPDPNLRMQAALALGEQRDVRAAGALVKALNDDDANVRYHAIEALG
                     KLKFADAVDTLAEIAVSKDFFLSFPALDALASIGDARVAPRIVPLLEDELLREPAVNL
                     LGQLGDESAVAPLTALLNTPSAPTDLIANALARIRDRYEAQYGEGGYIADLTTREISA
                     TGVQNLLDALETPGKEDLRSIALVLGWLKRTGVERALTRLMGRADLRDEIIDALVRHG
                     PATVDLLIGQLDSEDLEVRRSAVVALGRIGNSSATSHLVNTLSDQALAIEAANALAQI
                     GDPRAVDGLLNLLRNDDASIRQAAVNALNSLMPPAMSKRIIPLLHDSDPNVRESAVKI
                     AGYFGYPQSASDLLELSHDPVERVRCAAIEHLPYGEDEQIFEVLARAIKEETPTVRAA
                     AARALGNMDDPAAVPPLIDALSDDDVWVRYFSARALGHQRSENS"
     gene            complement(2009..>2542)
                     /locus_tag="VFO72_07885"
     CDS             complement(2009..>2542)
                     /locus_tag="VFO72_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479247.1"
                     /translation="SRALGEQAVAGDQVSKEANQLARQIGEISRAMSEQSKAAAEITM
                     ATQSMRLQSDQLSKAMREQARTSQEMTLAVGNISKDSLRITNSNRNHLDAAERIRAAV
                     TELRQITHRNADGVKATLTSTSGLARRAHELGEIMDKMVRTNAVTNGDQTGKAKGAKS
                     GSSRRARKAATENNSEN"
BASE COUNT          501 a          745 c          724 g          572 t
ORIGIN      
        1 cgctattctc agatctctgg tggccgagcg cccgcgcgct gaaatagcga acccaaacgt
       61 cgtcgtcgga gagcgcgtcg attaaagggg ggacggcggc cggatcatcc atattcccca
      121 gtgcacgagc ggcagcggca cggacagttg gcgtctcttc cttgattgcg cgagcgagaa
      181 cttcgaagat ctgttcatct tccccgtacg gcaggtgctc gatggcagcg cacctgactc
      241 gctcgacggg atcgtggctg agttccagta aatcactggc cgactgggga taaccgaagt
      301 atccggcaat cttcactgcc gattcgcgaa cgttcggatc cgagtcatgc agcagcggaa
      361 tgatacgttt ggacattgcc ggcggcatca gcgagttgag cgcgtttact gctgcctggc
      421 ggatcgatgc gtcgtcgttc ctgagcaggt tcagtagtcc atcgaccgcc cgcgggtctc
      481 cgatctgggc gagtgcgttt gccgcttcaa tcgcgagggc ttgatcgctc agtgtgttta
      541 caaggtgaga cgttgcgctc gagttaccga ttcgcccgag cgccaccacg gcggaccgtc
      601 tcacttccag atcttccgag tccaactgtc cgatcaatag atcgacagtc gccggcccgt
      661 gccgcacgag tgcatcgata atctcgtcac gcagatccgc acgtcccatc aatcgggtga
      721 gtgcgcgctc gacgccggtg cgtttgagcc agccgaggac cagggcgatc gagcgcaagt
      781 cctctttgcc cggtgtctcc aacgcatcga gcaaattctg aactccggtg gccgagattt
      841 cacgagtggt tagatccgcg atgtaaccac cttcaccata ttgcgcctcg tatcgatcgc
      901 ggatcctggc aagcgcgttt gcgatcaaat cggtcggggc agacggagta ttcaataatg
      961 ctgtcagcgg agctaccgca ctttcgtcac caagttggcc cagcaggttg acggcgggct
     1021 cgcgcaggag ttcatcttcc aacagcggca ctattcgcgg cgcgacgcgc gcgtcaccaa
     1081 tacttgcgag cgcatccaac gccggaaaag agagaaagaa gtctttggat acagctattt
     1141 cagcaagtgt gtctacagcg tcggcaaact ttagtttgcc gagagcctca atagcgtgat
     1201 agcggacgtt ggcatcgtcg tcgttcaatg cctttaccaa cgcgccggct gcacgaacat
     1261 cgcgttgttc acccaaagcc aacgcggctt gcatgcgcag attcggatcg ggcccctgca
     1321 acagttcgag taacggcgaa agagtgtcga cgtcactgga tgacaagatc tgaagcgcac
     1381 tattcagcaa cgacggattg tgatgattct cgactacgga gtttaagagc gcagcgatcg
     1441 cttcaggtgc agctcgctgc gacattccct tcaccgcagc acgccgaact ctccagcttt
     1501 cgtcactcaa cgcatcaagc aaaggcgctg cgtcataaaa ctcgtcaacg ctaaatgatt
     1561 cagccgcctc cagtcgcatc gcctcatcgc ccgacgctaa atgcgtagct agttcgcgct
     1621 cgcgttcgag ccgctcggtg acgtcttcga cagtgacaat caatccggcg atggtattgc
     1681 tgtcacgcag cggcgcgatg gttacgcgtt gctgcatttt gtcgaagtac ttagacgggc
     1741 ttacgggagc gcaggggatg aggtaatgat ggaaggcagg cgccagcact tccactacgc
     1801 ctttcctaag agctcgttgg aagcgtttca gcaacgcgcg cttttcaaga tcaggcagca
     1861 gcgcagtcaa agcccggcca gcggcagttt cgtcggggat gcccgtgagc cgcgccaggg
     1921 tggcgtccca gacccgaatg atcaagtcag cgtccgttgt aaaaacgccg acttcggact
     1981 gcgtttttgt gcccgcttgt gacaaaggtc agttttccga gttgttctct gtggctgctt
     2041 ttctggcgcg tctcgaactg ccggactttg caccttttgc tttaccggtc tggtcgccgt
     2101 tggtcacggc gttagttcgg accatcttgt ccataatctc cccgagttca tgcgcacgcc
     2161 tggccaaacc tgacgtactg gtcaaggttg ctttcacgcc atcggcattg cgatgggtta
     2221 tctggcgaag ttcggtgacg gcggcacgga ttctctccgc cgcgtccaga tggttacgat
     2281 tggaattggt gatgcgcaac gagtctttcg aaatattgcc aacggccaga gtcatttcct
     2341 gcgacgttcg ggcctgttcg cgcatcgcct tgcttaactg atcggactgc aagcgcatac
     2401 tctgcgtggc catcgtaatc tccgccgctg cctttgactg ctcgctcatc gctctgctga
     2461 tctcaccgat ctgccgcgcg agttggttag cttccttcga aacctgatct ccggcaactg
     2521 cctgttcgcc gagagcgcgc ga
//
LOCUS       DASTEY010000450         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1130536, whole genome shotgun sequence.
ACCESSION   DASTEY010000450 DASTEY010000000
VERSION     DASTEY010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130536"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /gene="ccmE"
                     /locus_tag="VFO72_07890"
     CDS             <1..372
                     /gene="ccmE"
                     /locus_tag="VFO72_07890"
                     /inference="COORDINATES: protein motif:HMM:NF009729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET9479248.1"
                     /translation="QQNLVFFFSPTQVVAKEAPVDRAFRIGGLVQDGTLKRDPDGLTV
                     RFTVTDTGASIPVVYKGILPDLFKEGRGCVAQGRIGPDGVFQAEQVLAKHDENYMPPE
                     AGEAIDKAKHAKEAAKTVVPN"
     gene            397..2415
                     /locus_tag="VFO72_07895"
     CDS             397..2415
                     /locus_tag="VFO72_07895"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET9479249.1"
                     /translation="MIPEIGQFALVIALLLALTQATLPLLGASRGNRTWIALAAPAGQ
                     AQFIFLTIAFCCLGYSFITNDFSVLNVATNSNSRLPLHYRLAATWGSHEGSLLLWTFM
                     LALWTVAVSLFSRHLPDEMVARVLSVMGIVSVGFLLFMLLTSNPFTRIFPVPPDGRDL
                     NPLLQDPAMVAHPPMLYMGYVGFSVAFAFAIAALIGGRLDATWARWSRPWTTVAWMFL
                     TCGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGGFKSWTV
                     LLAIAAFSLSLLGTFLVRSGVLTSVHAFATDPKRGVFILGFLVFVIGGSLLLYAWRAK
                     QVGLGSKFDVVSRESLLLSNNVLLTVAAGSVLLGTLYPLIVDALGMGKLSVGPPYFNL
                     VFVPLMAPAIFLMGVGPIARWKKAELPDLARRLRWAFVISVLSALTLPFVLGGWKWLV
                     SLGLLLAIWIVTTVFTNLWERVRVTSGQLSTWQKLMSQSRSYYGMQVAHLGVAVFIVG
                     VTMVTGYQSEQDVRMEIGDVVRAGGYEFQFNGVTDITGPNYVGARAEMIVNRNGVEKE
                     RMYPEKRNYTASGNVMTETAIDSGLFRDLYVSLGEPVGMGAWSVRVYYKPFVGWIWGG
                     AVLMALGGGLAVSDRRYALAARKEREAREAVDKPADVPATVAARTESV"
     gene            2447..>2686
                     /locus_tag="VFO72_07900"
     CDS             2447..>2686
                     /locus_tag="VFO72_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET9479250.1"
                     /translation="MLRYLIPLGLFLVLVVFLAIGLGRDPREVPSPLINKAAPTFRLP
                     QLKEPSKTFSAEDMRGKVWILNVWASWCVSCRDEHP"
BASE COUNT          504 a          707 c          815 g          660 t
ORIGIN      
        1 cagcagaacc tcgtcttctt tttcagccca actcaagtcg tcgccaaaga ggcaccggtc
       61 gatcgcgcgt ttcgcatcgg cggtctcgtc caggacggca cacttaaacg cgaccccgat
      121 ggtttgactg ttcgctttac ggtgactgac acaggggcaa gtattccggt ggtctataaa
      181 ggcattctcc ccgatctgtt taaggaaggc cgcggttgcg tggcacaggg aaggattgga
      241 cctgacggcg tgtttcaggc ggagcaagtg cttgccaaac acgacgagaa ctacatgcca
      301 ccggaggcgg gtgaagccat cgataaagcg aagcacgcca aagaagctgc caaaacggtc
      361 gtccctaatt aatctgtgaa gtccgtggct taattaatga tccctgaaat tggccagttt
      421 gcactcgtta tcgcactgct gctcgcgtta actcaggcta cgttgccgtt actcggcgct
      481 tcacgcggca atcgcacgtg gatcgcgctc gccgctccag ccggacaggc gcagttcatc
      541 tttctcacga tcgcgttttg ctgcctggga tactccttca tcaccaacga cttctcagtt
      601 ttgaacgtcg ccactaactc caattcacga ttaccgcttc attatcgtct cgcggcgacg
      661 tggggctcgc acgaaggttc gctgttgttg tggaccttca tgctggcgct ttggacagta
      721 gcggtttcac tcttcagcag gcatttgccg gatgagatgg tcgcacgtgt gttgagcgtg
      781 atggggatcg tgagtgtcgg tttcctgttg ttcatgttgc tcacgtcgaa tccgttcacg
      841 cgaatctttc cggtaccacc tgatgggcgc gacttgaatc cgttgcttca ggatccggca
      901 atggtggccc atccaccgat gctctacatg ggctatgtcg gtttctccgt cgccttcgcc
      961 tttgcgattg ccgctctgat tggcgggcga ctcgatgcga cgtgggcgcg ctggtcgcga
     1021 ccgtggacca ccgtcgcgtg gatgtttctt acttgcggta ttgcactcgg aagttggtgg
     1081 gcttattacg aactcggctg gggcggctgg tggttctggg atccggtcga gaatgcttct
     1141 ttcatgccgt ggctcgtcgg tacagcatta atccattcgt tggcggtcac cgaaaaacgc
     1201 ggcggtttca aaagctggac ggtgctgctc gcaattgcag cattttcgtt gagcctgctc
     1261 ggaacgtttc tcgttcgctc gggcgtgctg acgtcggtgc acgcgttcgc gaccgatccg
     1321 aagcgcggcg tcttcattct cgggtttctg gttttcgtga tcggcggatc gttactgctc
     1381 tacgcgtggc gcgcaaaaca agtcggactc ggaagcaagt tcgacgtcgt ctcacgcgag
     1441 tcgctgttgc tatcgaacaa cgttttgcta acggtggctg cgggttcagt attgctcgga
     1501 acattgtacc cgctgattgt ggacgcgctc ggcatgggca aactttccgt cggcccgcct
     1561 tactttaatc tcgtctttgt gccgctgatg gcgccggcga tatttctgat gggcgttgga
     1621 ccaatagcgc gttggaagaa ggcggaattg cctgacttgg caagacgact gcgctgggca
     1681 ttcgtgatca gcgtgctcag tgcgcttacg ctgccgtttg tacttggcgg atggaaatgg
     1741 ctcgtcagtc tcggactgtt actcgcaatc tggatcgtga cgaccgtttt cacaaacctg
     1801 tgggaacgcg tgcgcgtcac ttcgggccag ttaagcacgt ggcagaaact gatgagccag
     1861 agtcgcagtt actacggcat gcaagtggcg catctcggcg tcgccgtttt cattgtcggc
     1921 gtgacgatgg tgacaggtta tcagtcagaa caagacgtgc ggatggaaat cggcgatgtc
     1981 gtaagggcgg gtgggtacga gtttcagttc aacggcgtca ccgatataac gggaccgaac
     2041 tatgtcggcg cgcgcgcgga gatgatagtc aaccgcaacg gcgtcgagaa ggagcgaatg
     2101 tatccggaga aacgaaatta caccgccagc ggtaacgtga tgaccgagac tgcgatcgat
     2161 agcggactgt ttcgggatct ttacgtttca cttggcgagc cggtgggtat gggcgcatgg
     2221 agcgtacgcg tctattacaa gccgtttgtg ggctggatct ggggtggcgc ggtgttgatg
     2281 gcgctcggcg gcggcctggc ggtgagcgat cgtcgttatg cattggccgc gcgaaaagaa
     2341 cgcgaagcac gcgaagcagt tgacaaacct gccgacgtgc ccgccacggt cgccgcccga
     2401 accgaatccg tgtaatctgt atagtcagtg tagtctgtgg ttcaaaatgc tgcgttacct
     2461 aatacctctc ggcttattcc tggtgttagt ggtctttctg gcgatcggcc tgggccgcga
     2521 tccgcgcgaa gttccgtcgc ctttgatcaa taaggccgcg cccacgtttc gtctgccgca
     2581 actaaaagaa ccgagcaaga ccttttccgc cgaagatatg cgcgggaaag tttggatctt
     2641 gaatgtgtgg gcttcgtggt gcgtaagttg tcgcgacgag catccg
//
LOCUS       DASTEY010000451         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1179762, whole genome shotgun sequence.
ACCESSION   DASTEY010000451 DASTEY010000000
VERSION     DASTEY010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179762"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /gene="murF"
                     /locus_tag="VFO72_07905"
     CDS             complement(<1..1440)
                     /gene="murF"
                     /locus_tag="VFO72_07905"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET9479251.1"
                     /translation="MNVREAAALMGADASGISAAMFDNKITGFSIDSRSAGAGELFFA
                     LSQKDYTRAGFNGEFVDAHRFIRDAFQHGAVAAVARVDRVAGDPDLEVLRDRLMLVED
                     AIAALQHLAHRVYEAWNKPVVAITGSAGKTTAKELTAHVLKSAGHRVLKSERNYNNGL
                     GLPLSVLRMVSEGHSPEQFDLAVLEMGMSSPTHEIQRLCRITPPDVGVELMVAPVHLE
                     YLGTIENVAAAKAELIEGLKPEGTAILNADDALVMKMREKHAGRTLTFGIENTADVSA
                     TDIDARSLGRIGFRLLTPAGDARAQLIMSGQHNLSNALAAATVGVAFGLPAQQIADAL
                     STARPPKMRGEIIDFAAGFTVIDDSYNSNPRSLLNMVRTMAEAREGRKRLLVIAGEML
                     ELGPEEASLHRAAGREIAETGVNVLWGVRGLAKEIVAGAEEAGLSETSFFNSSEEAAA
                     EVVKEVKEGDLVLVKGSRGVATEKVINHLK"
     gene            complement(1727..>2148)
                     /locus_tag="VFO72_07910"
     CDS             complement(1727..>2148)
                     /locus_tag="VFO72_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9479252.1"
                     /translation="AAPIFREVAEQILPILGVMPDIETKSAPDMLAEAHQDSERAAKL
                     SEEQAQAEQERKATLPTVDSNGGRGGEVVYAVSRRKSMLMPDLRERSVRDVARTCAQL
                     GLQVEARGEGRVYKQSPVAGSEVSTGQTIYVDFRRPQ"
BASE COUNT          450 a          696 c          543 g          459 t
ORIGIN      
        1 cttcaaatga ttgatcactt tctcggtcgc aacgcctctc gatcccttca ccaagacgag
       61 atcgccctct ttaacttcct tcaccacctc cgcggcagcc tcttcagacg aattaaaaaa
      121 actcgtctca gagagtcccg cttcttcagc tcccgctact atctccttcg ccaggccacg
      181 cactccccaa agcacattca caccggtctc tgcaatctcg cgtccggccg cgcggtgcaa
      241 tgacgcttct tccggaccga gttccagcat ttctccggcg atcacaagca aacgtttgcg
      301 gccctcgcgg gcttcagcca tcgtgcgaac catgttcagc aacgaacgcg ggttcgagtt
      361 gtaagagtcg tcgatcactg taaagccggc agcgaaatcg atgatctcac cacgcatctt
      421 gggcggccgt gccgtactca gcgcatcggc gatctgctgg gccggcaaac caaacgccac
      481 accgaccgtc gccgcggcca gcgcgttgct caaattatgc tgaccactca ttattaactg
      541 cgcgcgcgcg tctccagccg gagtcaacag gcgaaaaccg atccggccca atgaacgcgc
      601 atcgatatcc gtagccgaaa catccgccgt gttctcgatg ccaaacgtca acgtcctgcc
      661 ggcgtgcttc tcgcgcatct tcatgactaa cgcgtcgtcc gcgttcaaaa tggcggtgcc
      721 ctcgggcttg agaccttcga tcagttctgc tttcgccgcc gcaacgttct cgatcgtgcc
      781 gagatactcg agatgaaccg gcgcaaccat cagctccacg ccgacgtcag gcggcgtgat
      841 gcgacacaaa cgctggatct catgagtcgg cgaagacatt cccatctcca gtacggcgag
      901 atcgaactgt tcgggtgaat ggccttcgct caccatccgc aaaactgaca acggcagacc
      961 gagaccgttg ttgtagttgc gctcactctt caacacgcga tgaccggcag acttcagcac
     1021 atgagcagtc aactccttcg cggttgtctt gccggcactg ccggtgatgg caacgactgg
     1081 tttgttccac gcttcgtaaa cacgatgcgc aagatgctgc agtgcggcaa tcgcatcttc
     1141 aaccaacatc agccggtcac gcagaacctc caagtcagga tcgccggcga cacgatcgac
     1201 gcgcgcgact gcggctacag ccccgtgttg aaacgcgtct ctgatgaaac ggtgtgcatc
     1261 cacaaactca ccattaaagc cggcgcgggt gtagtccttt tgcgaaagtg caaaaaagag
     1321 ttcgcccgcg ccggcagaac gggagtcaat cgaaaaacca gtaattttgt tgtcaaacat
     1381 cgcggcgcta ataccactcg catcggcgcc cattaaagct gctgcctctc gaacgttcac
     1441 gtgattacgg cctcttctcg ctcctctagc tgcgccgctg ccggttctgg cgacagttca
     1501 tccaactcag tttttggggg acgataatgc gcccggaggg ttgaacgctg actgcgtagt
     1561 cgtcgtctgc aatcagcaat gccgtagttt acgtcatttt caagcatctc ttcatctgca
     1621 cctgggaccg aaggcgtctc gcctgcacgg tgactaaacg ggcgttgccg tgagttgcag
     1681 gcgagacctc tgcggtccca ggcgcaggct gcatcagtag tggaactcat tgaggtctcc
     1741 ggaagtcaac gtaaatggtt tgtcctgtac tcacttcact tccggcgaca gggctttgct
     1801 tgtaaaccct tccctcgccg cgagcttcaa cctgtaatcc gagctgcgcg caggtccgcg
     1861 ccacatcgcg gacgctgcgc tcccgcaaat caggcatcag catcgacttg cgcctcgata
     1921 cagcgtaaac gacttcgccc ccgcggcctc cgttgctatc cacggtaggc aacgtggctt
     1981 tccgctcttg ttccgcctga gcctgctcct cgctcaactt cgccgcacgt tccgaatcct
     2041 gatgggcctc agccaacatg tcaggagctg acttggtttc aatgtcgggc atcacgccga
     2101 gaatcggcag gatctgttca gccacttcac gaaagatcgg agcggcca
//
LOCUS       DASTEY010000452         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1196617, whole genome shotgun sequence.
ACCESSION   DASTEY010000452 DASTEY010000000
VERSION     DASTEY010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196617"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VFO72_07915"
     CDS             complement(<1..1200)
                     /locus_tag="VFO72_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HET9479253.1"
                     /translation="MTRVLPVLFLLLLPLCVLAADETYTNPIIHADYSDPDVIRVGDD
                     FYLTASSFNCVPGLPILHSKDLINWRIIGHALQKQLPAEVFSQPQHGNGVWAPAIRFH
                     NGDFYIYYGDPDFGIYMVKARNAAGPWSQPLLIKAAKGWIDPAPFWDDDGNAYLVHAW
                     ANSRSGIKSILTINRMSADGTRLLDEGRTVFDGRVAHPTIEGPKLYKRNGYYYIFAPA
                     GGVTSGWQTVLRSKNIYGPYEDRIVMDRGRTMINGPHQGSWVELKSGESWFVHFQDRG
                     AFGRIVHLQPVNWINDWPVIGVDPDGDGKGEPVTTYRKPNVGREYPVQSPQTSDEFNA
                     GTLGLQWQWHANPQAGWLSFTERGGRLSLTAVPQPENGQNLWTVPNVLLQKLPAPDFL
                     VTTLVDAS"
     gene            complement(1197..1928)
                     /locus_tag="VFO72_07920"
     CDS             complement(1197..1928)
                     /locus_tag="VFO72_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET9479254.1"
                     /translation="MGEPNANIFKNIETDRRGTSFEDVVTQLREMIHRGELRPGDRLP
                     PERDLAKQLGVSRPTLRAGISSLAAVGVLQPRRGAGTFVVEAEGPPTLDSSPLRLMAS
                     LQRFTPAEMFEARRSLEMSIAALAAERATGDQMAAMSEEITGMFASLDEPEQFLVHDM
                     RFHQTVAAASGNRILTALMNMVATILFEVRSKTVRRARDLKESAEMHRAIYRAIRDRN
                     PEAARNTMRDHLMLAQAAQEQEEEN"
     gene            complement(1940..>2550)
                     /locus_tag="VFO72_07925"
     CDS             complement(1940..>2550)
                     /locus_tag="VFO72_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET9479255.1"
                     /translation="TGLLYHGYDESRKQRWANPTTGRSPNFWGRAMGWYAMALVDTLD
                     HFPKKHPQRGALVAILNRLARAVAKYQEPGSGLWYQVLDKGAEKGNYLESSVACMFVY
                     ALAKGVHKGYLPAAYMRVADNAYRGIVREFLKTDANGQLNLERTVSVGGLGGNPYRDG
                     SYEYYLSEKVITNDPKGVGALLLAATEMETRTTRTTGKKRVS"
BASE COUNT          518 a          741 c          705 g          586 t
ORIGIN      
        1 cgacgcatca accaaggtcg taacaagaaa atccggcgcg ggcagtttct gtaacaacac
       61 gttcggcact gtccacagat tctgcccgtt ctcaggctgc ggcacagcag tcaggcttag
      121 ccgcccgcca cgctccgtga acgagagcca tccagcctgc gggttggcat gccattgcca
      181 ctgcaagccg agcgtccctg cattaaactc gtcacttgtt tgcggcgact gcaccggata
      241 ctcgcgtccc acgttgggct ttcggtacgt cgtaacaggt tctccttttc cgtcgccgtc
      301 aggatccaca ccaatcaccg gccaatcgtt tatccaattg acaggttgca gatggacgat
      361 gcgcccgaac gcgccgcgat cctggaaatg gacgaaccac gactcacctg acttaagttc
      421 cacccacgag ccctgatgcg gaccattgat catggtccgg ccacggtcca tcacgatccg
      481 atcttcatac ggaccataaa tatttttcga gcgcagcacg gtttgccagc ctgaagtgac
      541 gccgccggcc ggcgcaaaga tgtagtaata cccgttgcgc ttatagagct ttggtccctc
      601 aatcgtcgga tgggcgacac gaccatcgaa gacggttctt ccttcgtcaa gcagtcgcgt
      661 gccatcggca ctcattcgat taatcgtgag gatgctcttt atgccggagc gactgttggc
      721 ccacgcgtga acgagatagg cgtttccgtc gtcatcccag aacggcgccg gatcgatcca
      781 tcctttagcc gctttgatta gcaacggctg cgaccatgga ccagcagcat tgcgcgcttt
      841 gaccatgtag ataccgaagt ccgggtcgcc gtaatatatg taaaagtcac cgttatgaaa
      901 gcggattgcg ggcgcccaaa ctccattgcc gtgctgaggt tgtgagaaca cttccgcggg
      961 aagctgcttt tgcagcgcat gcccgatgat tctccagttg atcagatctt tggagtggag
     1021 aatcggcaga ccgggaacac aattgaagct cgaagccgtg agatagaaat catcgccgac
     1081 gcgaattacg tccgggtctg agtagtcggc atggatgatc gggttggtat aagtttcgtc
     1141 agcagcaaga acgcacagag gcagaagaag gagaaataga actggtaaga ctctcgtcaa
     1201 ttttcttctt cttgttcctg tgccgcttgc gcgagcatga ggtgatcgcg catcgtgttg
     1261 cgcgcggctt cgggattgcg atcgcgaatc gcgcgataaa ttgcgcggtg catttcggcc
     1321 gactctttca agtctctcgc acgcctcacg gtcttgctgc gcacttcaaa caatatcgtt
     1381 gcgaccatgt tcatcaaagc ggtcagaatg cgattccccg acgcagccgc gaccgtttgg
     1441 tgaaagcgca tgtcgtggac gagaaactgt tccggctcat cgagcgaggc aaacatccct
     1501 gtgatctctt ccgacatcgc agccatctgg tcgcctgtcg cacgctccgc ggcgagagcc
     1561 gcaatcgaca tttccagcga gcgtcgcgct tcaaacatct cggctggtgt gaaccgctgc
     1621 aacgaggcca tcaacctgag cggactgcta tcgagtgttg gcgggccttc ggcttcgacg
     1681 acgaaagttc cggcgccgcg gcgtggttgc aggacgccga ccgcggcaag ggaactgatg
     1741 cctgcgcgca acgttggtcg actgactccc agttgctttg cgagatctct ttcgggcggc
     1801 agacgatcgc cgggacgtaa ctcgccgcga tgaatcattt cgcggagctg cgtaacgacg
     1861 tcctcgaaac tcgtaccgcg acgatccgtc tcgatgttct tgaaaatgtt tgcgtttggc
     1921 tcgcccattt gctcctttct tagcttaccc ttttcttgcc tgttgtacga gttgttcgcg
     1981 tctccatctc cgttgccgcg agcagcaaag cgccgacgcc ctttggatcg ttggtgatca
     2041 ctttctcact gaggtaatac tcgtaactgc cgtcgcggta gggatttccg cccaggccgc
     2101 cgacgctgac ggtgcgttcc aggttgagtt ggccgttggc gtcagtttta agaaactctc
     2161 ttacgattcc tcgataagcg ttgtccgcca ctctcatgta agcggccggc agatagccct
     2221 tgtgcacgcc tttggcgagc gcgtaaacaa acatgcaagc caccgatgat tcgaggtagt
     2281 ttcctttttc cgcgcctttg tccaacacct gataccacag cccggaacct ggttcctggt
     2341 acttcgcaac agcgcgcgcg aggcgattca gtatagcgac aagcgctccg cgctgcgggt
     2401 gttttttcgg gaagtggtcg agcgtatcga cgagcgccat tgcgtaccaa cccatcgcgc
     2461 ggccccagaa gtttggcgag cgtccggttg ttggattggc ccaacgctgc ttacgagatt
     2521 cgtcgtagcc gtgatagagc agaccggttt
//
LOCUS       DASTEY010000453         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_174879, whole genome shotgun sequence.
ACCESSION   DASTEY010000453 DASTEY010000000
VERSION     DASTEY010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174879"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..596)
                     /locus_tag="VFO72_07930"
     CDS             complement(138..596)
                     /locus_tag="VFO72_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9479256.1"
                     /translation="MSNPQPVSAEAKRVAGVIVTHGHLAGELLAAAEMIVGPISHIAA
                     VSIGWHDDVDAARDEVQRAITRVSEGAGVLLLTDMFGGTPTNIASMFLEEGVIEVVTG
                     VNLPMVIKLATSTAEESLAEIAPKIRDQGRQGIYLAGALLATPPTKSGDD"
     gene            complement(589..1524)
                     /gene="rapZ"
                     /locus_tag="VFO72_07935"
     CDS             complement(589..1524)
                     /gene="rapZ"
                     /locus_tag="VFO72_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET9479257.1"
                     /translation="MVVPDIVIITGLSGSGMSSATNAFQDLGYFCVDNLPLTMLPTFG
                     RLVTTDDDEKGIRRAALVIDIREREFLADFEKQLEALRALGLNVTVLFFEASDDVLQY
                     RFSETRRPHPADKGPGLLVAISAEREAMSSIRALADQIIDTSDHTVHTLRSFLLERFS
                     PDRQGAPMRVQVMSFGHKYGNPGDLELLFDVRHLPNPHFVPELKPLSGHDSRVVRYLR
                     SQPEVQETLKRFTDLLEYLLPLYKREGKSYVTVGIGCTGGRHRSVMVANALARALRRG
                     GFDAHASHRDVRKAVHRLHRKKSVESAAGGRSDNE"
     gene            complement(1610..2146)
                     /gene="raiA"
                     /locus_tag="VFO72_07940"
     CDS             complement(1610..2146)
                     /gene="raiA"
                     /locus_tag="VFO72_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9479258.1"
                     /translation="MKFEYTGRHVEVSPAIRRHVEDHFKKLDHIFNGDSTLNTHVIID
                     VEKNRQIGEIIVNWRDHTLTAKDTNADMYMALTRAMAKIEKQAVKLKKKIIDRKQGAK
                     SVARVAPAPDGDIEATPRPPRIIPARRYAVKPMTAEEASLRLSGEPDQFLVFRDADTQ
                     RLGVIYKRKDGNYGLIEP"
     gene            complement(2283..>3390)
                     /gene="rpoN"
                     /locus_tag="VFO72_07945"
     CDS             complement(2283..>3390)
                     /gene="rpoN"
                     /locus_tag="VFO72_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET9479259.1"
                     /translation="APTFEQFLSRPPSLAEHLEWQLHMTSLAPEVVEAAIAVIGNLDA
                     DGRLNATNEEIAAMQNCSEEIVETARQAVMRLDPVGCGARDVKESLLVQLEVRGESDR
                     LAARLISDHFADLQQHRLPHLSKQIGIDVETLLEELHFIRTLDPYPGRRYSSEEPILI
                     SPEIYIEKLDENDDEYIIYFADDGSPRLRVSQQYQQMLSQGVSNETKSFIREKMRSAV
                     DLLRNIEHRRQTIYKVVESIVRRQKDFLDHGVQHIKPMMLKDIAEDIGMHLSTVSRVV
                     NRKYAHTPQGVIELRRFFTEGMLNEEGEEISTRIIKLQIKKLIEEEDSHNPITDDQVV
                     KILAKDGIKLSRRTVAKYRDQMQIPGSRERRAVV"
BASE COUNT          711 a          967 c          928 g          784 t
ORIGIN      
        1 gtcggcctca agatcgcctt ccagtcgctg tagcgtgagt ttgcttttga atctgctcgc
       61 cacgcgtacg aggttggcgg cggcacgagc gtgaagaccg agacggctct ttacctcgat
      121 agtgcgttcg gccacgttta gtcgtctccg gacttggtgg gcggcgtcgc gagcaacgcg
      181 ccggcgaggt aaatcccctg gcgtccctgg tcgcgaatct taggcgcgat ctcggcgagc
      241 gactcttccg ccgttgaggt tgcgagtttg atcaccatcg gcaggtttac accagtgacg
      301 acttcgatca cgccttcttc gagaaacatt gacgcgatgt ttgtcggcgt gccgccgaac
      361 atgtctgtca gcaacagcac gccggcgccc tcggaaacgc gcgtgatcgc acgctgcact
      421 tcgtcgcgtg cggcgtcgac gtcgtcgtgc caaccgatgg aaactgccgc aatgtgcgag
      481 attggtccaa cgatcatttc ggcggctgcg agcagttcgc ctgcgaggtg accatgcgtc
      541 acgatcacgc cggcaactct ttttgcttcc gcagagactg gttgaggatt actcattgtc
      601 gcttctaccc ccagccgcag attccacaga tttctttcgg tgtaatctgt ggacggcttt
      661 ccgcacgtcg cggtgcgatg cgtgcgcatc aaacccgccg cggcgcagtg cacgcgccag
      721 ggcattcgcc accatcacgg atcgatgccg cccgccagtg caaccaatac cgaccgtaac
      781 gtacgacttc ccttctcgtt tgtagagggg caagaggtac tcgagcagat ccgtaaatcg
      841 tttcagcgtc tcctgcactt ccggttgcga ccgcagatat ctgacaacgc ggctgtcgtg
      901 ccccgacaac ggtttcaact ccggcacaaa atgcgggttc ggcagatggc gcacatcaaa
      961 caataactcc agatcgcccg ggttgccata cttatggccg aagctcatca cctgaacgcg
     1021 catcggcgcg ccctgccggt caggactaaa tcgctccagt aaaaaactgc gaagcgtgtg
     1081 aacggtgtga tccgaagtat cgatgatctg atccgcgagc gcgcgaatgc tcgacattgc
     1141 ttctcgctcg gcactgatgg ccactaacag gcccgggcct ttgtcggctg gatgcggacg
     1201 gcgtgtttcg ctgaaacggt attgcagaac gtcgtcggaa gcttcaaaaa agagaacggt
     1261 gacgttcagg cccaacgcgc gtaaggcttc gagctgtttt tcgaagtcag cgagaaactc
     1321 gcgttcgcga atgtcgatga ctagtgcagc gcgcctgatg cccttttcgt cgtcgtcagt
     1381 agtgacgaga cgtccgaaag tcggaagcat cgtgagtggg aggttgtcga cgcagaaata
     1441 tcccagatcc tggaaagcgt tggtggccga cgacatcccc gaaccactca aaccggtaat
     1501 gatgacgata tccggaacga ccatcagacg tggacaggat tgacagagtt tacaagactt
     1561 aggctaaaac aatcctgtat gtttgtaatc ctgtctctgt ttgcctcggt cacggctcga
     1621 tgagcccgta gtttccatcc ttccgtttgt aaattacacc caaacgttgt gtgtccgcat
     1681 cgcggaacac gagaaactga tcgggctcgc ccgaaagccg cagggacgct tcttccgcgg
     1741 tcatgggttt caccgcgtaa cgccgcgcgg gaatgatccg cggcggacgc ggcgtggctt
     1801 caatatctcc atcaggggca ggagcaacgc gcgcgaccga cttggcgccc tgcttgcgat
     1861 cgataatctt cttcttgagt ttgactgcct gtttctcaat cttcgccatg gcccgcgtca
     1921 gcgccatgta catgtccgcg ttggtgtctt tcgcggtcaa cgtatgatcc cgccagttga
     1981 cgataatctc gccaatctgg cgattcttct cgacgtcaat gattacgtga gtattcaggg
     2041 ttgaatcgcc gttgaaaatg tggtccagtt tcttgaaatg atcttcaacg tgacgccgaa
     2101 tggccggcga cacttcaacg tgacgccccg tgtattcgaa tttcatcagc acctcctcga
     2161 actggtgaaa caaaagagac caatgcgcca gcctggctgt cgtgacttcg cgaagggagc
     2221 cggggctcga cgcaacaaca gttataacta aaactggaaa agcgcgccgg tgtttgaggg
     2281 gcttaaacga cagcgcgacg ctcgcgcgac ccggggatct gcatttgatc gcgatacttt
     2341 gcgacagtgc ggcgcgataa ctttattccg tcctttgcca gaatcttaac cacctggtca
     2401 tcggttatcg gattatgcga atcttcctct tcgatcagtt ttttaatctg taatttaata
     2461 atgcgcgtcg aaatctcctc accctcttca ttcaacatgc cttcagtgaa gaagcggcgt
     2521 aactcgatca cgccctgcgg cgtgtgcgcg tacttacgat tcacgacgcg cgacactgtc
     2581 gacaggtgca tgccgatatc ttcggcaatg tctttcaaca tcatcggttt gatgtgctgc
     2641 acgccgtggt cgagaaagtc tttctgtctc cgcacgattg actcaacgac tttgtagatc
     2701 gtctgccggc ggtgttcgat gttccgcagc aggtctaccg ccgaacgcat cttctcgcgg
     2761 atgaaactct tcgtctcgtt gctcactccc tggctaagca tctgctgata ctgctggctc
     2821 acacgcaagc gcggactgcc gtcgtcagca aaatagatta tgtactcgtc gtcgttctcg
     2881 tcgagcttct caatgtagat ttccggactg atgagaatcg gctcctcaga ggagtaacgc
     2941 cggcccgggt aaggatcgag cgtgcgaatg aagtgcaact cttccagcag ggtttcgacg
     3001 tcgatgccga tctgcttcga aaggtgcggc aagcgatgtt gctgtaagtc ggcgaaatga
     3061 tcgctgatca aacgcgctgc cagccgatcg ctttcaccac gcacttcgag ttgcaccagc
     3121 agggactcct taacgtcgcg cgcgccacag cccaccggat cgagtcgcat aacagcctgc
     3181 cgggccgttt cgacgatctc ttcggagcaa ttctgcatcg ctgcgatctc ttcgttcgtc
     3241 gcattgagac ggccgtccgc gtcaagatta ccaattacag caatggcagc ttcaacgact
     3301 tccggtgcga gagaagtcat atgcagttgc cattcaagat gttcggcaag tgagggcgga
     3361 cgactcaaaa actgttcaaa tgtaggcgcg
//
LOCUS       DASTEY010000454         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_322960, whole genome shotgun sequence.
ACCESSION   DASTEY010000454 DASTEY010000000
VERSION     DASTEY010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322960"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFO72_07950"
     CDS             complement(<1..277)
                     /locus_tag="VFO72_07950"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET9479260.1"
                     /translation="MSKSEPARDQRLFIHNLVKRDPLAGLAEDVRRGLEAQPKRFLPK
                     YFYDELGSQLFEAICLLPEYYLTRAENEILKRYADEIAASVDGQITLV"
     gene            356..2047
                     /locus_tag="VFO72_07955"
     CDS             356..2047
                     /locus_tag="VFO72_07955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479261.1"
                     /translation="MFFRQLFPALLSALLLTIFSGSALAQSFKQELEAPEKVELTVRN
                     LDGRVSVVASDEQHKKVTVDARSSGDAIDPSDVKIETKGASIVIDVRSRGEKNRIDVV
                     VTIPPRSKVDVEGKAGAVDIIGNVESATVKTDTGTIHADVPLEALKFDFVWEASRPRF
                     MSDVELPPIKEKAGGMFKLSGRLGEKDKKKEEVVALNFRTQRGVVLLNVDPEMAPADL
                     RERPLTEAARAIVRSGDSQLVEAIRKVSPKMFGDYAKTLPPPEKEPELVRRTPPSQIV
                     SSVSPQLLRFNASVTDRNGRAISGMRESDFTVWENGIERRVTDVSPANEPFNLVLLLD
                     VSGSVEERMDFIRKAARDFLRTASPQDRISIISFRDDIQVISDFSTDRQMLSRKLDEI
                     DAGGGTALYDALGYVLSEPLRRLRGERTAIVVMSDGDDNKSFLPFPAILEALSESGAL
                     VYPLYVPSGLIPESSVPKPEITIDPTRTRYLTLTTRAEDEGRKLATASGGIYYPIRRL
                     EDLQKAYDDIVAQLRSAYTITYASNSISNSPRVRVRINRDGVSVRLSPVVGLNTP"
     gene            2059..3327
                     /gene="xseA"
                     /locus_tag="VFO72_07960"
     CDS             2059..3327
                     /gene="xseA"
                     /locus_tag="VFO72_07960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9479262.1"
                     /translation="MNQPLFSALFDETERKPLTVTQLTNSIRISLESRFASVWVEGEI
                     SNFKDHSSGHWYFTLKDQNAQLRAKCFRSTNTRIRFRPANGLHVRARGKLSVYAPRGE
                     YELVIDALDPVGAGALRIAFEQLRDRLQAEGLFAKELKRPLPVFPRRVGIVTSPTGAA
                     IRDILNVISRRTRTVHVLFSPARVQGDSAGPDIARAIRFINQYHERALREGRTADLVD
                     VLIVGRGGGSTEDLWAFNQEEVARAIRKSMIPVISAVGHETDFTIADFVADLRAPTPS
                     AAAELVAAREDEICSALDHLSRQMTRLMRFRIVDLRSRVQEQALSHAFDEAKSRLRNA
                     RRRFDAASATCNDAMEASLQQARERLGLAAASLDALSPLAVLQRGYAIAQDASGKPLR
                     DATSVNAGDEVAVRLAKGRLNTRVETVEES"
     gene            3351..>3906
                     /locus_tag="VFO72_07965"
     CDS             3351..>3906
                     /locus_tag="VFO72_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479263.1"
                     /translation="MAKRIVINVEPGRKTPVRRKSSRWLRILGLLAILVVVVIGVAAA
                     GAFFWWRHYQSTPAYSLTLLVDAAQRGDAEELAKRIDEEEVARNMVEIVSKKANARYG
                     LSMNEARQQQIDKVMPSLLPRLKQTIHDEVAKEIKTFAATSESKSFIFLLMTVPSLVT
                     ITTERDVAKAAAAVSNRTIELTMRR"
BASE COUNT          854 a         1070 c         1157 g          825 t
ORIGIN      
        1 caaccagcgt gatctggccg tcaacggagg cagcaatctc gtccgcatat cgtttgagaa
       61 tctcgttttc agcgcgagtc agatagtatt cgggaagcag gcagatggct tcgaagagct
      121 gcgatccgag ttcgtcgtaa aagtatttgg gcaaaaagcg ctttggctgg gcctccagac
      181 ctctgcgcac gtcttctgcg agtccggcga gcggatctcg cttcacaagg ttgtgaataa
      241 aaagacgttg atctcgggca ggctccgatt ttgacaagcg ggtttttcct cgccgttatg
      301 atagcccata atccgcgctt gaccactacg cgcgcggcgg gcaagatcgg tcgcgatgtt
      361 tttcaggcaa ttgtttccag cgctgctttc tgcacttctg ctcaccattt ttagcggttc
      421 cgcactcgcc cagagtttca aacaggagct cgaggctccg gaaaaggtcg agttaactgt
      481 gcgtaatctt gacggccgcg tcagtgttgt cgcctccgac gaacaacaca aaaaagtcac
      541 cgtcgacgcc aggtcttccg gcgatgcgat cgatccgagc gatgtcaaga tcgaaaccaa
      601 gggcgccagc atcgtgatcg acgtgcgatc gcgcggcgaa aagaaccgca tcgacgtcgt
      661 tgtaaccatt ccgccacgtt cgaaagtcga cgtggaaggg aaggccggcg cggtggacat
      721 aattggcaac gtcgagtcgg cgaccgtgaa aaccgacacc ggaactattc acgcggatgt
      781 gccgctcgaa gccctgaagt tcgacttcgt ttgggaagcg agccgcccgc gtttcatgag
      841 cgacgtcgag ctgcctccga tcaaggaaaa ggctggcggc atgttcaaac tttccggccg
      901 tctcggggaa aaggacaaaa agaaagaaga agtagtcgcg ctcaactttc gcacgcaacg
      961 cggcgtggtg cttttgaatg tcgatccgga aatggcgccg gcggatctgc gcgaacggcc
     1021 gttgaccgaa gccgcgcgcg caattgtacg tagtggcgac agtcaactcg tcgaagcaat
     1081 tcgcaaggtt tcgccgaaga tgtttggcga ttacgcgaag acactgccgc cgccggaaaa
     1141 agaaccggag ctggtgagac gcacgccgcc gagtcaaatc gtgagttcag tctcgccgca
     1201 gttgctgcgt ttcaatgcca gcgttacaga tcgcaacggc cgagcgatca gcggcatgcg
     1261 cgagtcagac tttacggtgt gggaaaacgg aatcgagcgc cgcgtgacgg acgtttcgcc
     1321 ggcgaacgag ccgttcaatc ttgtgttgct gctggacgtc tccggcagcg tcgaagagcg
     1381 aatggacttc attcgcaaag cagcgcgcga ttttctgcgc acggcgagcc cgcaggaccg
     1441 catctcaatc atcagttttc gcgacgatat tcaggtaatt tcagatttta gtaccgaccg
     1501 gcagatgctg tcgcggaagt tggacgagat cgacgccggc ggcggtaccg cactttacga
     1561 cgcgctcgga tacgttttga gtgaaccgct aagacgcttg cgcggcgaac gcaccgcgat
     1621 cgtcgtgatg tctgacggcg acgacaacaa atcgtttctg ccgtttccgg cgattctcga
     1681 agcgctgtca gaatcgggcg cccttgtgta tcccctgtac gttccttccg gcttgatacc
     1741 ggaatcgagt gtgccgaaac cggagatcac gatcgatcca acacgcacgc gttacctgac
     1801 cttgaccacg cgcgctgaag acgaaggccg caaactcgcg acagcttcgg gcggaatcta
     1861 ctatccgatt cgccgcctcg aagacttgca gaaagcgtac gacgatatcg tggcgcagtt
     1921 gcgctcggcc tacacgatca catacgcgtc aaactcgatt tcgaattcgc ctcgcgtgcg
     1981 cgtgcgcata aaccgtgacg gcgtttcggt gcgactcagt cccgtggttg gtttgaacac
     2041 accttgaaga atccctcgat gaaccaaccc cttttcagcg cgctttttga tgaaacggaa
     2101 cgaaagccgc taacagtcac ccaactcaca aactcgattc gcatctcgct cgagtcgcgt
     2161 tttgcctcag tgtgggttga aggcgagatt tcgaatttta aagatcattc atccgggcac
     2221 tggtacttca cgctgaagga ccaaaacgcg caactccgcg cgaagtgctt ccgttcaacc
     2281 aacacgcgca tccggtttcg tccggcgaat ggtctacatg tgcgcgcgcg tgggaagctt
     2341 tcagtctacg caccgcgcgg cgagtatgaa ctcgttatcg acgctttgga tcccgtcggc
     2401 gccggcgcgt tgcggattgc gtttgaacag ttacgcgatc ggttgcaggc tgaaggtctt
     2461 ttcgcgaagg agttgaaacg cccgctgccc gtctttccgc gacgtgttgg tatcgttacc
     2521 tcaccgacgg gcgctgcgat tcgcgacatt ctcaacgtta tctcgcggcg cacacggacg
     2581 gtgcacgtgc tgttttcacc cgcgcgtgtg cagggcgaca gcgcgggtcc ggacattgcc
     2641 cgcgccatac gtttcatcaa ccagtatcac gaacgcgcgc tacgcgaagg acgcacggcg
     2701 gatctggttg acgttttgat tgtcgggcgt ggcggtggtt cgactgaaga tctgtgggcg
     2761 ttcaaccagg aagaggttgc gcgcgcgatt cggaagtcaa tgattccggt catctcagca
     2821 gtgggccacg aaactgattt cacgattgcc gacttcgtcg ccgatctgcg cgcgccgacg
     2881 ccgtctgctg ctgcggagct cgttgcggca cgtgaagacg agatctgttc ggcgttggac
     2941 cacctcagca ggcagatgac gaggctgatg cgttttcgca tcgtagattt gcgcagtcga
     3001 gtacaggaac aggcgctgtc gcacgcgttt gacgaagcga agtcgcgttt gcgaaacgcg
     3061 cgccggcgtt ttgatgcggc gtcggcaacc tgcaacgacg cgatggaagc aagtcttcag
     3121 caagcacgag agagacttgg tctggcggcg gcttcgctcg acgcgctttc gccgctcgct
     3181 gtgttgcaac gcggctacgc aatcgcgcag gatgcatcag gcaaaccgtt gcgcgacgcg
     3241 acctcagtta acgctggaga tgaagtagcc gttcgactcg caaaggggag attaaacacg
     3301 cgcgtcgaaa ccgttgagga gagctaatcg gtattcgtta gtgtgaatcc gtggccaaaa
     3361 gaattgttat taacgttgag cccggtagaa agacgccggt tcgtcgcaaa tcttcacgct
     3421 ggctgaggat acttgggctg ctggcgattc tcgtcgtggt cgtaatcggt gtggccgcag
     3481 ccggcgcgtt tttctggtgg cgacactatc aatcgacacc ggcttactcg cttacgttgc
     3541 tcgttgatgc agcccaacgc ggcgatgcgg aggaacttgc caagcgaatc gacgaagaag
     3601 aagtcgcgcg aaacatggtc gagatcgtaa gtaagaaagc gaacgctcgt tacggtctgt
     3661 caatgaacga agcgagacag cagcagatcg acaaagtgat gccttcgtta ttgccgcggc
     3721 tgaaacagac gattcatgac gaagtcgcta aagagatcaa gacttttgct gccacgtcag
     3781 aatcgaagtc gtttatcttt ctgctgatga ctgtgccgtc gctggtaact atcacgacgg
     3841 aacgcgacgt ggcgaaagcg gcagccgcag tgagcaatcg aacgatcgaa ctaacgatgc
     3901 gtcgcg
//
LOCUS       DASTEY010000455         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_438464, whole genome shotgun sequence.
ACCESSION   DASTEY010000455 DASTEY010000000
VERSION     DASTEY010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438464"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFO72_07970"
     CDS             complement(<1..1162)
                     /locus_tag="VFO72_07970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET9479264.1"
                     /translation="MKANCWYGKTVTRVEDVPEPQILNQRDAIVKISSTAICGSDLHL
                     YNGFIPTMQRGDILGHEFMGEVVEVGREVTNLKIGDRVVVPFPIACGNCFFCQSEMHS
                     LCENSNPNAWMAEKMFGHAASGIFGYSHLTGGYAGGQAEYARVPFADVGPIKVPDHLT
                     DEQVLFLSDIFPTGYMGAEMCNIKPGDTIAIWGCGPVGQFSIRSAFLLGAERVIAIDE
                     VPERLQMAEQGGAETIDFSKHNVYEVLMDMTGGRGPDACIDDVGMEAHAPGLIHAYDR
                     MKQAMMLETDRPHALREAIMCCRNGGTISVIGVYGGFIDKFPIGSVMQRSLTIKTGQA
                     HVQRYMRPLLQMIENGDIDPSFVVTHVMSLEDAPRGYELFKNKQDGCIKIVLK"
     gene            complement(1162..2190)
                     /locus_tag="VFO72_07975"
     CDS             complement(1162..2190)
                     /locus_tag="VFO72_07975"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9479265.1"
                     /translation="MSATPQPFSNERSYAAERSEPGMPKSGPKRLAKGLGWFSVGLGL
                     AELLAPQAIAKISGVSNKHTSLIRLYGLRELASGITIFTQKKPATGVWSRVAGDALDL
                     ASLGMAFTAPDAKRGRVAFATANVLAVTALDLMCATQLSNGTHGVHAQASCIVNRAPE
                     EVYQFWRHFENLPRFMKHLESVEDLGDGRSHWTAKGPAGTTVEWDAMIVADVHGEVIT
                     WRSLEDSDVDNAGAVRFERAAGNRGTIVKVNIQYNPPAGVLGATVAKLFGEEPEQQLD
                     DDLRRFKQVLEVGEVVVSDATLMGTGYFQQRPARPADIDELEEAESTQALPSQTATNY
                     DGAEGRMF"
BASE COUNT          495 a          732 c          658 g          578 t
ORIGIN      
        1 gctttagcac gatcttgata catccgtcct gcttgttttt gaacagctcg tatccgcgcg
       61 gcgcgtcttc gagactcatg acgtgagtca cgacgaaact cggatcgatg tcgccgttct
      121 ctatcatctg caacaacggt cgcatgtatc tctgcacatg cgcttggccc gtcttgatcg
      181 tgagcgaacg ttgcattacg gaaccaatcg gaaacttatc gatgaacccg ccgtagacgc
      241 caatcaccga gatcgttccg ccgttgcgac aacacatgat cgcttcacgc aacgcatgcg
      301 gccgatccgt ttccagcatc atcgcctgct tcatccggtc gtacgcatgg atgagcccgg
      361 gtgcgtgtgc ttccatgccg acgtcgtcga tgcacgcatc aggaccgcga ccgccggtca
      421 tgtccatcag cacttcgtaa acgttatgtt tgctgaagtc gatcgtttcc gcaccgccct
      481 gctcggccat ttgtaaacgc tccggcacct catcgatcgc gatcactctc tccgcgccaa
      541 gcaagaacgc gctgcgaatt gaaaactgtc ccaccggacc gcaaccccag atggcgatcg
      601 tatcgcccgg tttgatgttg cacatctcag cgcccatgta accggtcgga aagatgtcgg
      661 aaagaaacag cacctgctcg tctgttaaat gatccggcac tttgatcgga cccacgtccg
      721 cgaacggcac tcgcgcgtat tccgcctggc cgcctgcata gccgccggtc agatgtgagt
      781 agccaaaaat tcccgaagcg gcatggccaa acatcttttc ggccatccag gcattcggat
      841 tcgagttttc gcaaagcgaa tgcatttcac tctggcagaa aaagcaattg ccacacgcga
      901 tcggaaacgg tactacaacg cggtcgccga tcttcaggtt tgtcacttcg cggccaactt
      961 ctaccacttc gcccatgaac tcgtgaccga gaatgtcgcc tcgctgcatt gtcggaatga
     1021 atccgttgta aaggtgcaag tcggaaccac agatcgcggt cgagctgatc ttcacgatgg
     1081 cgtcgcgctg gttgagaatc tgtggctcgg gaacgtcttc cacgcgcgtt acggttttgc
     1141 cgtaccaaca gtttgctttc atcagaacat cctcccttcc gcgccgtcgt agttcgtcgc
     1201 tgtttgactt ggcaacgcct gcgtcgactc ggcctcttcg agttcatcga tgtccgccgg
     1261 gcgggcaggt cgctgctgga aataacccgt gcccattagc gtggcgtccg acactactac
     1321 ttcgccgact tccagcactt gtttaaagcg tcgcagatcg tcgtctaact gttgttccgg
     1381 ttcttcgccg aaaagctttg cgactgtagc gccgagcacg cccgccggag ggttgtattg
     1441 aatattgact ttgacgatcg tgccgcggtt gccggcagct ctctcgaaac gaaccgcgcc
     1501 ggcgttgtcg acgtcagagt cttcgagtga acgccacgtg atcacttcac cgtgtacgtc
     1561 ggcgacgatc atcgcgtccc attcgactgt cgtgccggcc ggaccttttg cagtccagtg
     1621 agagcgaccg tcgccgaggt cttccaccga ctcgagatgt ttcatgaagc gcggcaggtt
     1681 ttcgaagtgt cgccagaatt ggtaaacctc ttcaggcgca cgattgacga tgcaactcgc
     1741 ttgcgcgtgg acgccgtgcg taccgttgct gagctgtgtg gcgcacatca gatccagtgc
     1801 ggtaaccgcg agcacgtttg ccgtggcgaa agcgacgcga ccgcgtttcg cgtccggcgc
     1861 ggtaaaagcc atgccgagac ttgcaagatc caatgcgtcg cccgcgacgc gtgaccaaac
     1921 accggtggcc ggcttctttt gcgtgaagat cgtaatgcct gatgctaatt cacgcaggcc
     1981 gtacagtcgt atcaaactgg tgtgtttgtt tgaaacaccg gaaatttttg cgattgcttg
     2041 tggagcgaga agttctgcga gaccaagacc gacactgaac cagccgagtc ccttcgcgag
     2101 tcgtttggga cccgacttgg gcatgcctgg ttcggatcgc tcggctgcat agcttctttc
     2161 gttcgagaat ggttgtggtg tggcgctcat aaatgtcccc ccttgactga acgttgtagt
     2221 ttggattggt ttgtcagttc aggagaacgc actcaaaccc tatgcctcgc cctcaggctc
     2281 cttcgcaccg gcctgcgtcg ttccgcggtt cgtttgccgt acctgcttcg ctccgtagga
     2341 gcaaaatgtt tatagcgcga cgaacatcca catcgcgctc cttcggagcg caatctttaa
     2401 aggcgtgcac gagctataaa catcttgctc ctacggagcg aagcagtatc ggaggaacag
     2461 aac
//
LOCUS       DASTEY010000456         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_108777, whole genome shotgun sequence.
ACCESSION   DASTEY010000456 DASTEY010000000
VERSION     DASTEY010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108777"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="ribA"
                     /locus_tag="VFO72_07980"
     CDS             <1..562
                     /gene="ribA"
                     /locus_tag="VFO72_07980"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET9479266.1"
                     /translation="PTETGEFRIIGFRSLVSAEEFVVLVKGELRPDRSSLVRIHSQCM
                     TGDVFGSIKCDCGRQLSAAMKLIEQEGHGAIVYQQQEGRGIGIINKIRAYALQDEGAD
                     TIEANERLGFEADMRRYEQCAEILLELGLRAVRVMSDNPAKIRALRQAGLKVVERVPL
                     AVEPHDFFAGYLKTKKEKMGHLCSVA"
     gene            570..2171
                     /locus_tag="VFO72_07985"
     CDS             570..2171
                     /locus_tag="VFO72_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9479267.1"
                     /translation="MNDKDVHVANTSDLRDGEMKEISAGETRILLARVGDRFHAVSPT
                     CPHYGAPLVEGALCGTRIVCPWHHAVFNIVNGDLEEPPALDALVSYAVRIEGEQVLVS
                     VPEDMPDRRTPTMATCDPAADSRQFVIIGAGAAGYAAAQTLREEGFRGNVVMITREHH
                     TPYDRPNLSKDYLHGHAEPEWIPLRSDEFFTEHGIQLVLNREVTRVDAHAKTITFESG
                     ETMDYDALLVATGGAPIRLNIPGSDLKNICMLRSFADSDLIIETAKSSRRAVVVGSSF
                     IGMEAAYSLRERGLEVTVVAPSQEPFETTLGTEVGALFRRLHESHGVRFKLGSIVYHF
                     EGSHNVEAVTLDNGEQIETDMVVVGAGVRPVTHFIEGVELDDAGGIVVDSRLCAADGI
                     YAAGDIASFKDPRTGERMRIEHWRTAQQQGRAAARNMIGRDVAFEGVPFFWTRQFDVG
                     LLYVGHAKSWDEIIFHGEVSSHDFLAFYLRRGRVQAVAGMNRDREMAVAEELLRLGRM
                     PAREQLKWDDISLVKILHAPELINV"
     gene            2239..>2919
                     /locus_tag="VFO72_07990"
     CDS             2239..>2919
                     /locus_tag="VFO72_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9479268.1"
                     /translation="MITVAGSATLSRSIQPGRVQIFSGLEAEEMSRIAGHARSLRKAR
                     GEFIYMPGDRADFVYILRQGRVKLSVLSESGKEVAIDIIQPGEIFGEFALVDESPRSN
                     MAQALDDMLMWVFSKRDFTQLLAAQPKLALSYIRVVGDRRRRMEKKLSDITSKAVSAR
                     VCELLHELATSSAEVDTIASDYLVPLTHHDVASLIGAARQTTTTVLNDMERRSIIELG
                     RGWIRIKRL"
BASE COUNT          627 a          820 c          872 g          600 t
ORIGIN      
        1 gccaacagag acgggagagt ttcgcatcat tggttttcgc tcgcttgtct ccgcggaaga
       61 gttcgtcgtg ctcgtgaaag gtgagttgcg acccgaccgg tcaagccttg tacgtattca
      121 ctcgcagtgc atgaccggcg acgtgtttgg ctcaatcaag tgtgactgcg gccggcaact
      181 cagtgctgcc atgaaactca tcgagcagga aggacacggc gcgatcgtct accagcaaca
      241 agaagggcgc ggcatcggca tcatcaacaa gatccgcgct tatgccctgc aagacgaagg
      301 cgctgacacc atcgaagcca atgagcggct gggcttcgaa gctgacatgc ggcgctacga
      361 acaatgcgcc gaaatcctgc tcgagcttgg cctgcgcgcg gtgcgcgtga tgtcggacaa
      421 tccggcaaag atacgcgcgc tgcggcaggc cggtcttaaa gtcgtcgagc gtgtgccgct
      481 cgcggttgag ccgcacgatt tcttcgccgg ctatctaaaa acgaagaagg agaagatggg
      541 ccacctttgt agcgtggcct gacgaacaga tgaatgacaa agacgttcac gtagcgaaca
      601 cctccgacct gcgggacggc gagatgaaag agatatcggc cggtgagacg cggatcttgc
      661 tggcacgtgt tggcgatcgt ttccatgcag taagtccgac gtgtcctcac tatggcgcgc
      721 cgctcgttga aggtgccctc tgcggcacgc gcatcgtttg cccgtggcac cacgctgttt
      781 tcaacatcgt caacggcgat ttggaagaac cacccgcact cgacgcgctc gtcagttacg
      841 cagtgcgcat tgagggcgaa caggtcctgg tcagtgttcc tgaagacatg ccagatagac
      901 gcacgcccac aatggcaacg tgcgaccccg ctgccgactc gcgacagttt gtcatcattg
      961 gcgctggtgc ggctggttac gccgctgctc aaacacttcg cgaagagggt tttcgcggca
     1021 acgtggtgat gatcacgcgc gagcaccata cgccctacga tcgccctaac ctcagcaaag
     1081 actatttgca cggccacgcc gagccggagt ggattccact ccgttccgac gagtttttca
     1141 ccgagcacgg cattcagctc gtgctgaaca gggaggtcac gagagtagac gcgcatgcga
     1201 agacgattac attcgagagt ggtgagacga tggactacga cgcgctactg gttgcaacag
     1261 gcggcgcgcc gattcgtctc aatatcccgg gttcagatct aaaaaacata tgcatgctgc
     1321 gcagtttcgc cgactccgat ttgatcatcg agacagccaa gagctcacga cgcgcggtgg
     1381 tagtcggttc gagcttcatc ggaatggaag cggcgtacag tttgcgcgag cgtggccttg
     1441 aagtaaccgt tgtggctccg tctcaggagc ctttcgagac aacactcggc acggaagttg
     1501 gtgcgctatt ccggcggttg cacgagagtc atggtgtgcg cttcaagctt ggctcaatcg
     1561 tctatcactt tgagggcagt cacaacgtcg aagccgtgac gctcgacaat ggcgaacaaa
     1621 tcgagacaga tatggtcgtc gtcggggcag gcgtgcggcc agttactcat ttcattgaag
     1681 gcgtggagtt ggacgatgcg gggggcatcg ttgttgattc gcggctgtgc gcggctgacg
     1741 gtatttacgc cgccggcgac atcgccagct ttaaggatcc acgcactggc gagcgcatgc
     1801 gcatagagca ctggcgcact gcgcagcagc aagggcgtgc agccgcgcgc aacatgatag
     1861 gacgcgacgt cgcattcgaa ggcgtcccat tcttctggac acgacaattc gacgttggac
     1921 tcctctacgt tggccacgcg aagtcgtggg acgagatcat attccatggc gaggtctcgt
     1981 cgcacgattt tcttgctttc tacttaaggc gcggtcgtgt gcaggcagtg gcaggcatga
     2041 accgcgatcg cgagatggca gtcgccgagg agttgctgcg gctcggtcgc atgccggcgc
     2101 gcgagcagct caagtgggat gacataagtc ttgtaaaaat acttcatgca ccggagttga
     2161 taaacgtttg atgcttcgtt aactttaggt aagagttgcc cagtactcca aaatccacct
     2221 cccggtagga gtctgtaaat gataacggtc gcagggtccg cgacgctttc tagatctatt
     2281 caacctggcc gtgtgcaaat cttctcgggc ctcgaggccg aagagatgag ccgcatcgcg
     2341 ggacacgcgc gcagtctgcg caaggcgcgc ggggaattca tctacatgcc cggcgatcgc
     2401 gccgactttg tctacatcct gaggcaggga agagtaaagc tgtcggtgct ctccgagtcg
     2461 ggcaaagagg ttgcaatcga catcatccag cccggcgaga tcttcggtga gttcgcgctc
     2521 gtagacgagt cgccgcgcag taacatggcg caggcgctcg acgacatgct catgtgggtc
     2581 ttttcgaagc gcgactttac acagctcctc gcagctcaac ctaaactcgc tctgagttac
     2641 atccgagtcg tcggcgatcg tcgccggcgc atggagaaga aactttcgga cattacttcg
     2701 aaggctgtct ccgcacgcgt ctgcgaactt ctgcacgagc tcgccacgag ctcagccgaa
     2761 gttgacacca ttgccagcga ttacctcgtg ccgcttacac atcacgacgt cgcgagtcta
     2821 atcggcgccg cgcgccaaac cacgaccacc gttctcaatg acatggaacg tcgcagcatc
     2881 atcgaattgg gtcgtggttg gattcgcatc aagcgtctc
//
LOCUS       DASTEY010000457         5686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_471468, whole genome shotgun sequence.
ACCESSION   DASTEY010000457 DASTEY010000000
VERSION     DASTEY010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471468"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..2483
                     /locus_tag="VFO72_07995"
     CDS             102..2483
                     /locus_tag="VFO72_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9479269.1"
                     /translation="MKLQKPVLLLLLALIAFARPVQSNDPVSAADTPMPSEVFGFAPG
                     DDRKLASWSQVLDYFEQLDRASDRVIFETLGQTSMGKSFVMATISAPDNLARLEEYKQ
                     IQAQLADPRKLGPPATRDRKAATLIKRGKTIVLITCGIHSTEVGSYLSSTLIAHRLAS
                     SNDPDIQKILANTIVLLVPSLNPDGVDIVKNWYDKTLGTPFEGTGPPELYHKYTGHDN
                     NRDWYAFTQVETQLTVDKIHNVWHPQIVHDIHQQGAMGSRLFLPPYMAPVEPNVPKPI
                     IEGFTKLGNWMAREMRAKGFQGITTNSTYDAWTPARAYSHYHAGVRMLSETASAKIAS
                     PLTLKFDELTSREGYDPQKESVNFGPVWRGGEWHLRDITNYMTTAAFLLLQHAAENRV
                     EWLRSFYGIGKEAVRPRRPAELFGFLIEPSANAPNLVAILNRGGVEIETPKEGTTLRI
                     GKRTFPEGTKIVRMDQPYGAFAKALLEVQRYPNLRDAAGHPKAPYDVTAHTLPLLMGV
                     EVHAVRAPFRYARATEPRTEVVGARNAVAWSPGTPLRGLYRSSVPAYDEGWTRWIFDT
                     FLTIGYPYRTITDKDLRERVLVYKPSPGVTIKYPAIVIPDQSARTLLEGYRSGAMPPE
                     HTGGIGADGVRTLREFVETGGTVVFLNRASNFAIEQFKLPVRNVVAGLPRTEFYVPGS
                     ILRIELDTSHPLAHGMPKQTIAWAEDSPVFEVAEVAGASLPPSNVRVIGSYPADQNPL
                     LSGWLLGAERLKGKAALVEVQMGEGRIILFGFRPQYRAQSLATFPLFFNALSR"
     gene            complement(2554..4242)
                     /locus_tag="VFO72_08000"
     CDS             complement(2554..4242)
                     /locus_tag="VFO72_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET9479270.1"
                     /translation="MNALIRVDGNNNGLKPAPVQPPKHTREVRPLSEEMVDEMLAGRG
                     FRGWLRAARVARVLGLFSLYLFLDTYDVRADWNRRAVTRLRDRARERGRIAQLKAWAW
                     TQFYAAFDLFIRALRYLIFRGAEGSARKEARLEKQAVWMRESLINLGPTFIKIGQALG
                     TRADLLPLAYVKELSRLQDQVPAFPTAEAFARIEAELGRPLHECYPEIDSEPIAAASL
                     GQVYRARLATGEEVAVKVQRPNLEATISFDIAILFRLVKLTNRFFPRANENADWEGML
                     REFHATIFEEMDYVKEGRNADRFRYNFRTWRAVRVPKIHWSHTNTRVLTLDFIRGTKV
                     VDLEGLRANGISAVKVNRLLIRTYLKQLLEDGFFHADPHPGNLLVMDSGHLAFFDFGM
                     VGRITPKLQTQMIDAFFHVVGRDVHGLGQDIINLNFLKPGVDPETVRPVVESLFTHYL
                     NLKLGEVNFKELTYDLAEVIYEYPFRLPANFTYVMRALMTLEGIGVVTDPGFSFFETA
                     KPFAREFMLRREGKMLRKLIIDKLTGRENGDGQIAWSRVWKLTKMAARMYLEKQ"
     gene            complement(4252..5205)
                     /locus_tag="VFO72_08005"
     CDS             complement(4252..5205)
                     /locus_tag="VFO72_08005"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET9479271.1"
                     /translation="MRLFHGTDNAEIQRPTVLTLGVFDGLHLGHQLIMSTVVDRARAT
                     GAVPTVITFDPHPRAVLHPESAPPLLQTFDQKIEGFGVLGIEQTIVVRFTKEFSNVSA
                     EEFIKDVVMDRLHAREVYLGKGFAFGHNREGNIELLKRLGGELGFVAGEVPEVKLRGK
                     RVSSSKIRELLARGQVNLARRMLGRPYGVEGRVERGSERGHKLGFPTANLHPHNRVIP
                     RNGVYVTGTLLDGQWRRSVTNVGLRPTFGDASEPSVETFVLNWDGDLYGDVVRVRFLY
                     RLRDERKFSSVAELKRQIATDVARAESYFERAGTKHMLSVI"
     gene            complement(5302..>5686)
                     /locus_tag="VFO72_08010"
     CDS             complement(5302..>5686)
                     /locus_tag="VFO72_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9479272.1"
                     /translation="QVIPYTVFHRAPFCIGPELEITPLEVIHGRLPVMAYRFNDFAYL
                     TDLSEIPPDTIDSLRDLDVLVLDCLRFREHPTHLWVDRALEYIEQIRPRRTFFTHIAH
                     DIKHARDSARLPADVAFAHDELVVE"
BASE COUNT         1259 a         1652 c         1574 g         1201 t
ORIGIN      
        1 cgcggggttt gaaaacgctc gctactttac acgggcataa cgcgctcggc aagcggtact
       61 tacggggttt tttccgcgtt tttggtgata aactgcgcgg gatgaaactc caaaaacccg
      121 ttctgctact gctcttagcg cttatcgctt tcgctcgtcc ggtacagtcg aacgacccgg
      181 tttcagcagc tgacactccg atgccgtcag aggtgttcgg gtttgcaccc ggcgacgatc
      241 ggaaactcgc ttcatggagc caggtactcg attatttcga gcagttggat cgcgcgagtg
      301 accgcgtgat ctttgaaacg cttggccaga cgtctatggg caagtctttc gttatggcca
      361 ccatcagcgc gcccgataat ctcgcgcggc tcgaggaata caaacagatt caggcgcagc
      421 ttgctgatcc gcgtaaactt ggaccacccg caacacgcga tcgcaaagcc gcgacgttga
      481 ttaagcgcgg caaaacgatt gtgctgatca cgtgcggcat tcactcgacc gaagtcggtt
      541 cgtatctgtc gagtacgctc atcgcgcacc ggctcgcgtc gtcaaacgac cctgacattc
      601 aaaaaatact cgcgaacacg atcgtgctgc tggttccatc actgaatccc gacggcgtgg
      661 acatcgtgaa gaactggtac gacaagactc tcggtacgcc cttcgaagga accggtccgc
      721 cggagctcta tcacaagtac accggacacg ataacaatcg cgactggtac gcgtttacgc
      781 aggtcgaaac gcagctcact gtcgacaaaa ttcacaacgt ttggcatcca cagatcgtcc
      841 acgacattca ccagcagggc gccatgggat cgcgcttatt tctgcctcct tacatggcgc
      901 cggttgagcc aaacgtgccg aagccgatca tcgaagggtt cacaaagctc ggcaactgga
      961 tggctcgcga gatgcgcgcc aaaggctttc agggcattac caccaattcg acgtacgacg
     1021 cgtggactcc ggcgcgcgca tattcgcact atcacgccgg cgtgcgcatg ttgtcggaaa
     1081 cagcgagtgc gaagatcgcg tcgccgctga cgttgaagtt tgacgaactc acgtcgcgcg
     1141 aaggttacga tccgcagaag gagtcggtga actttggacc agtatggcgt ggcggcgagt
     1201 ggcacctgcg cgatatcacg aactacatga cgacagcagc gtttctgttg ttgcagcatg
     1261 cggcggagaa tcgcgttgag tggttgcgtt cgttctatgg catcgggaaa gaagccgtgc
     1321 gaccgcgaag accggcagaa ctgtttggtt ttttgatcga gccatcggcg aatgctccga
     1381 acctagtagc gatcctcaat cgcggtggag ttgaaataga gacgccgaaa gaaggcacga
     1441 cgttgaggat tgggaagcgc acttttcccg agggaacgaa gatcgtgcgg atggaccagc
     1501 cttacggcgc gtttgcgaaa gcgttattgg aggtgcagcg ttatccgaac ctgcgcgacg
     1561 ccgcgggcca cccgaaagca ccttacgacg tgaccgccca cacgctgccg ctattgatgg
     1621 gcgttgaagt tcatgctgtg agggcgccgt tcagatacgc gcgtgcgaca gaaccacgga
     1681 cagaggtggt tggcgcgaga aatgcggtcg cgtggtcacc aggcacgcca ctacgcgggc
     1741 tttaccgttc gtcggtgcct gcgtatgacg agggttggac cagatggatc ttcgacacct
     1801 tcttaacgat cggctatccc taccgcacga ttactgacaa agacctgcgc gaacgtgttc
     1861 tggtttataa gccgtcacct ggtgtgacga tcaaatatcc cgcgatcgtc attccggacc
     1921 agtctgcccg aactctgctt gaaggctatc gcagcggagc tatgcctccc gaacacaccg
     1981 gcggcatcgg agctgacggt gttagaaccc ttcgtgagtt tgtcgagacc ggtggaacag
     2041 tcgtgtttct gaatcgcgct tcgaactttg ccatcgaaca gttcaagttg cccgtgcgga
     2101 acgttgtggc aggtttgccg cgtacggagt tttacgtgcc tggttccatt ctgcgcattg
     2161 agctcgacac gtcgcatccg ctcgcgcatg gaatgccgaa acagacgata gcgtgggccg
     2221 aggattcgcc ggtatttgag gtcgcggaag ttgcaggggc cagcctgccg ccgtccaacg
     2281 ttcgtgtgat cggttcgtac cctgctgacc agaacccgct tctctcgggt tggttactgg
     2341 gagcggagcg gctcaagggc aaggcagcgc tggttgaagt gcaaatgggg gaaggccgca
     2401 tcattctgtt cggcttccgc ccgcagtacc gcgcgcagtc gttggcgacg tttccactgt
     2461 tcttcaacgc cctgagccgg tagttctcgc gttcggcgcg gaagggcgcc ggagatacag
     2521 accggggccc cggtccgtgc aatctgtggc taactattgc ttctccaggt acatgcgtgc
     2581 agccatcttc gtgagcttcc acacacgaga ccacgcaatc tgaccgtcgc cattttcgcg
     2641 accggtgagc ttgtcgatga tcagtttgcg cagcatcttg ccttcacgcc gcagcatgaa
     2701 ctctcgcgca aacggtttcg ccgtctcgaa gaaactgaaa cccggatcgg tgacgacgcc
     2761 gattccttcc aacgtcataa gagccctcat gacgtaagtg aagtttgccg gcaagcggaa
     2821 cggatactcg taaatcactt cagcgaggtc gtaagtcagt tccttgaaat taacttcacc
     2881 gagcttcagg ttcaaataat gcgtgaacag actctcaacc accggacgaa ccgtttccgg
     2941 atcgacaccc ggcttgagga agttcaaatt gatgatgtcc tggcccaggc cgtggacgtc
     3001 acgaccgacg acgtggaaga acgcgtcaat catctgcgtc tgcaacttcg gagtgatacg
     3061 accaaccatg ccgaagtcga agaacgcaag atgaccgctg tccatcacca gcaagttgcc
     3121 cggatgcgga tcggcgtgga agaaaccgtc ttcgagtagt tgcttgaggt aagtccgaat
     3181 cagcaggcga ttaaccttga cggctgagat accgttcgcc cgcaagcctt cgagatcgac
     3241 caccttcgta ccgcgaataa aatccagcgt caacacgcgt gtgttggtgt gtgaccagtg
     3301 gattttcggc acacgaaccg cgcgccacgt gcggaagttg taacggaacc tatccgcgtt
     3361 gcgaccttcc ttgacgtaat ccatctcctc gaagatggtg gcgtgaaact cacgcagcat
     3421 tccttcccaa tcggcattct cattcgcgcg cgggaagaac cggttcgtca gctttacgag
     3481 ccgaaacaga atcgcaatgt cgaaactgat agtcgcttcg agattcggac gctgcacttt
     3541 gacggcgacc tcttcaccag tcgccagtcg agcacgatag acctgtccca acgaagccgc
     3601 ggcgatcggc tcactgtcga tctccggata gcattcgtga agcgggcggc cgagttccgc
     3661 ttcgattcgc gcaaacgcct cagcagtcgg aaacgccggg acttgatcct gaagtcttga
     3721 tagttctttg acgtacgcca gcggcagcag atccgcgcgt gtgcccagcg cctggccaat
     3781 cttgatgaac gttggaccaa gattgatcaa tgattcgcgc atccaaaccg cctgcttctc
     3841 gagtcgagct tcctttcttg ccgatccttc agcgccgcga aagatcaagt aacgcaacgc
     3901 gcggatgaag agatcgaaag cggcgtagaa ctgcgtccac gcccaggctt tgagttgtgc
     3961 gattcgcccg cgctcgcgag cgcgatcgcg aaggcgcgtg accgcccggc gattccagtc
     4021 ggcgcggacg tcatacgtat cgagaaacag atacagcgag aagagaccga gcacgcgcgc
     4081 tactcgcgcc gcacgcaacc aaccgcgaaa accgcggccg gccagcattt cgtcaaccat
     4141 ctcttccgag agcggtcgaa cctctcgtgt gtgctttggc ggttggactg gcgcgggttt
     4201 cagaccgtta ttattgccgt ctactctgat aagtgcgttc attcgtaata actagatcac
     4261 cgataacatg tgtttcgtgc ccgcacgctc aaaataactc tccgcgcgag ccacgtctgt
     4321 cgcaatctgc cttttcaact cagctactga actaaacttt cgctcgtcac gcaagcgata
     4381 caaaaatctc acacgtacaa catcgccgta gagatcgccg tcccagttca gtacgaacgt
     4441 ctccaccgaa ggttccgacg catccccgaa cgtcggtctc agcccaacgt tagtgacgct
     4501 gcggcgccat tggccgtcaa gcaaagtacc ggtgacgtaa acaccgttcc ggggtatgac
     4561 gcggttgtgt ggatgcaggt tggccgtcgg aaaacccaac ttatggccgc gttctgagcc
     4621 tctttcgacg cgtccttcga cgccgtacgg acggcccaac atgcgtcgcg ccaggttcac
     4681 ctgcccgcgc gccagcagct cgcgaatctt gctcgaactc acgcgcttcc ctcgtaactt
     4741 aacttccggc acctcgcccg ctacgaaccc gagttcgcca cccagccgtt tgagcagttc
     4801 gatgtttcct tcacggttgt gaccaaaggc aaatcccttg cccaggtaaa cctcgcgcgc
     4861 gtggagccga tccatcacca cgtctttgat aaattcttcg gcgctgacgt ttgaaaactc
     4921 tttcgtgaaa cgcacgacga tcgtctgctc gatccccagc acaccgaaac cttcaatctt
     4981 ctgatcgaac gtctgtagca atggcggcgc agactccgga tgcaacaccg cacgcggatg
     5041 cgggtcgaac gtgatcactg tcggcacagc accggtggct cgagcacgat ccacaacagt
     5101 gctcataatc aactggtggc ccaggtgcag accatcaaac acgccaagcg taagcacggt
     5161 cgggcgttga atctccgcgt tgtcagtacc gtgaaaaaga cgcatagagc attttcgatt
     5221 gccgattgtc gattgccgat tgctcaaagt tgcctattgg ccttctcaat tggaaattgg
     5281 caatcaatcg gaaatcggca atcactccac caccagttcg tcatgtgcaa acgcgacatc
     5341 tgccggcagg cgcgccgagt cgcgtgcgtg cttgatatcg tgcgcaatgt gcgtgaagaa
     5401 cgtgcgccgc ggtcttatct gctcgatgta ctccaaagcc cgatcgaccc agagatgtgt
     5461 cggatgctct cggaaacgca aacagtcgag cacgagcacg tcgagatctc gcaacgaatc
     5521 gatcgtgtcc ggcggtattt cgctaagatc tgtaagatac gcaaagtcgt taaagcgata
     5581 agccattacg ggaagccggc cgtggatcac ctcgagcggc gttatttcca attccggtcc
     5641 gatgcaaaat ggagcgcggt gaaagaccgt gtaaggaatc acctgc
//
LOCUS       DASTEY010000458         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_323075, whole genome shotgun sequence.
ACCESSION   DASTEY010000458 DASTEY010000000
VERSION     DASTEY010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323075"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /gene="hisD"
                     /locus_tag="VFO72_08015"
     CDS             complement(<1..746)
                     /gene="hisD"
                     /locus_tag="VFO72_08015"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET9479273.1"
                     /translation="MISIVRADEIGELMARLAARSVAFDSDLLKQVTSIIDDVRQRGD
                     KALIDYTRRFDGVELAPAELRIREETLDRSAAKVDEKILAALRVAIRNVRAFHERQLE
                     QSWEISPTEGVRLGQRITPLERVGLYVPGGTAAYPSSIVMNVVPAQVAKVERIVVTTP
                     PRTLAENPAIAAALLELNVDEIYGVGGAQAIAALAFGTETVPRVDKITGPGNKYVAAA
                     KKILFGVVGIDAIAGPSEVVIIADETGRAE"
     gene            complement(743..1381)
                     /gene="hisG"
                     /locus_tag="VFO72_08020"
     CDS             complement(743..1381)
                     /gene="hisG"
                     /locus_tag="VFO72_08020"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET9479274.1"
                     /translation="MITIAIAKGRMQNDALTLLAHAGVRLDDDARNSRRLALTDESGN
                     YRFIFVKPADVPVYVEHGIADCGVVGRDVLLESQADLLLPLDLRIARCRIVVAAADAE
                     IMTRSLGMLRVATKYSQIAGAHFGARGIPVEIIQLSGSVELAPALGLADLIVDLVETG
                     RTLRENGLMVVEEIAESTGRLVVNRASYQLKSESITRLVKALETAVEGGQRA"
     gene            complement(1378..>2156)
                     /locus_tag="VFO72_08025"
     CDS             complement(1378..>2156)
                     /locus_tag="VFO72_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9479275.1"
                     /translation="LLALRPDVTSSVARMAATLLSERPRPLRFCYAAPVFRQQPQSHA
                     EWRRENTQLGCELIGVGGHAADLEVLRLAAEILAQLDLQSSYCITINNVEIFNGVAAH
                     LNLPAAAREQLRRLIDTRETAELGHFLKTYANGESRAFLQATQLTGKHDVIETARSVI
                     TNSRSVAALDSLEELWTAIESLGLADRFEIDLSDVSSLDYYTGLSLKVFVHGAGTSVG
                     RGGRYDGLTGNFGRAEPAVGFVLNLDALTEVLARRMVGIS"
BASE COUNT          453 a          696 c          565 g          442 t
ORIGIN      
        1 tactccgctc gtcccgtttc atccgcaatg atcacgacct cgctcggacc ggctatcgcg
       61 tcgattccga cgacgccgaa aaggattttc ttcgcagcag caacgtactt gtttcccggt
      121 ccggttatct tatcaactcg cggaactgtc tctgtcccaa acgccagcgc cgcaatcgcc
      181 tgcgcgccgc caaccccata gatctcatca acgtttaact cgagcagcgc tgccgctatt
      241 gcaggattct ccgcgagtgt acgcggcgga gtcgttacca caatccgttc aactttcgct
      301 acctgcgccg gcacgacatt cataacgatc gacgacggat acgccgcagt accgcccggc
      361 acatacagac caactcgctc cagaggtgta attcgctggc ccaggcgtac gccttccgta
      421 ggcgaaatct cccaggactg ttcgagttgc cgctcatgaa atgctctcac gtttctgatc
      481 gccactcgca gcgcggcaag tatcttttca tcaaccttcg ccgctgaacg atcaagtgtc
      541 tcttcacgga ttcgcagttc ggcaggcgca agctcgacgc catcaaatct tctggtgtaa
      601 tcaatcaatg ccttgtcgcc acgctggcgg acgtcgtcga taatcgaagt cacctgtttg
      661 agtagatcgg agtcgaacgc cactgaccgc gccgccagcc gcgccatcag ttcaccaatc
      721 tcatccgcgc gcacgatcga aatcaagccc tttgaccccc ttcgacggcc gtctccaaag
      781 cttttactag ccgtgtgatc gattccgatt tgagttggta actcgctcga ttaaccacta
      841 gccgtccagt cgactccgca atctcttcaa ctaccatcaa tccgttctcg cgcaatgttc
      901 ggcccgtctc caccaggtcg acaatcaaat ctgcgagacc taaggccggc gccaattcca
      961 ccgaaccgga aagttgaatg atttcgacag gaatgccacg cgcgccaaag tgcgcgcccg
     1021 cgatctgcga atacttggtg gcgacgcgca acatgccaag gcttcgcgtc atgatctccg
     1081 catcagccgc ggccacaact atgcgacagc gtgctatccg gaggtccaac ggcaacaaca
     1141 gatccgcctg cgattcgagc agcacgtcgc gccccacgac gccgcagtcc gcaatgccat
     1201 gttcgacgta gacgggaaca tcggccggct tcacgaaaat gaaacgatag ttgccggatt
     1261 cgtcggtgag tgcaagtcgt cgtgaattcc gcgcgtcgtc atccagccga acgccggcgt
     1321 gcgccagcag tgttagcgcg tcgttctgca ttcttccttt ggcgatcgcg atcgtgatca
     1381 tgaaatgccg accattcttc gagcgagaac ttcagtgagc gcgtcgagat tgagcacaaa
     1441 accgaccgcg ggttcggcgc gtccgaagtt tccggtcaga ccatcgtaac gcccgccgcg
     1501 accgacactc gtgccggcgc cgtgcacgaa cactttcaga ctcagacccg tgtagtagtc
     1561 gagactcgaa acatcgctga gatcgatctc aaaacgatcg gcgagtccga gcgattcgat
     1621 tgcggtccac agctcttcga gcgaatcgag tgcagccacg gatcgtgagt tggtgatgac
     1681 gctgcgcgca gtttcgatga cgtcgtgctt tccggtcagt tgcgtcgctt gcaggaacgc
     1741 tcggctttcg ccgttcgcgt aagtcttgag gaaatgcccc aactccgcgg tctcgcgagt
     1801 atcgatcagg cggcgtaact gctcgcgtgc ggccgccggc aagttcagat gcgcagcgac
     1861 accattgaag atctcgacgt tattgatcgt aatgcaataa ctcgattgca gatccagttg
     1921 cgccaggatc tccgccgcca gccgcagcac ttcgaggtcg gcggcgtgac cgcccacgcc
     1981 gatcaactca catccgagtt gcgtgttctc acgccgccat tcagcgtgcg actgcggttg
     2041 ctgcctgaac acgggagccg cgtaacagaa acgcaacggg cgcgggcgct cggacagcaa
     2101 cgtggccgcc atacgcgcga ccgagctcgt aacatccggc ctgagtgcta acagcc
//
LOCUS       DASTEY010000459         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_784727, whole genome shotgun sequence.
ACCESSION   DASTEY010000459 DASTEY010000000
VERSION     DASTEY010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784727"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFO72_08030"
     CDS             complement(<1..647)
                     /locus_tag="VFO72_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9479276.1"
                     /translation="MKLIILAAGYATRLYPLTLNQPKPLLPVAGKPMLEHVLDNISTI
                     PDIDHAYIVTNAKFVSHFENWAQTYQRPALHFGFTIVNDGSTDDSNKLGAIGDLHLVL
                     QKHEVNEDIIIVGGDNLFSNDLREFGDYCKEKLAPVTGVYDVGDLEQIKKYNAIEIDE
                     NSRIIYFEEKPQIPRSTLTGIALYYYPKWTLSLIDQYIAEGNNPDQPGRLVQWLYP"
     gene            complement(647..1771)
                     /locus_tag="VFO72_08035"
     CDS             complement(647..1771)
                     /locus_tag="VFO72_08035"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET9479277.1"
                     /translation="MSQTHIHNSPRTFRAPGRVNLIGEHTDYNDGFVMPAAIDLSVFA
                     RVWPRHDRKLAIRSENFDDEIEFDLDQPNPAPGRHWSDYAVGVAVMLERAGHRLRGAN
                     LQIRGEVPVGSGLSSSAAVEVATACALTSVSELNVDRRELALLCQKAENEFVGARVGI
                     MDQFVALFGQAQMALLLDCRSLEFKLLPLPDTVNLVICNTMVKHALAASAYNERRAQC
                     EAGVNHLGRFLPHVKALRDVTIEQLEEFGRDLPDVVYSRCRHVITENARVLAAGEALE
                     KRDLHRFGELMAQSHRSLRDDYEVSSSELDLMVELANTVEGVHGARMTGGGFGGCTVN
                     IVDAVHVEQFKQSVAREYQQAMAVNPEIYVCEPSNGAEEI"
     gene            complement(1749..2012)
                     /locus_tag="VFO72_08040"
     CDS             complement(1749..2012)
                     /locus_tag="VFO72_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479278.1"
                     /translation="MTRIFLALMLLQALTPLQEMVRTEQTFSRMAAEKTTREAFMAFI
                     ADDGLLFRPGAVNGKKWMLEHPVPPSDKRPLLRVVLDVTNPHP"
     gene            complement(2009..>2344)
                     /locus_tag="VFO72_08045"
     CDS             complement(2009..>2344)
                     /locus_tag="VFO72_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9479279.1"
                     /translation="VQQPAGSLSGGNQQKVVVARLLHQDADVLLLDEPTRGIDIGSKA
                     QVYETIAECAAQNKAVLMVSSYLPELFGMCDRLAVMSRGRLSEVRPIDRWTPESVLQA
                     AIGATDKHE"
BASE COUNT          507 a          689 c          622 g          526 t
ORIGIN      
        1 gggtacagcc actggaccag gcggcccggc tggtccggat tgtttccttc ggcgatgtac
       61 tgatcaatca gcgacagcgt ccatttcgga tagtagtaaa gcgcaattcc cgtcagcgtg
      121 ctcctgggga tttgcggttt ctcctcgaaa tagatgatgc gactgttttc gtcgatctcg
      181 atcgcgttgt atttctttat ttgttcgaga tcgccgacat cgtagacgcc tgtgaccggc
      241 gcgagttttt ccttgcagta gtcgccgaac tcgcggagat cgttgctgaa caggttatcg
      301 ccgccgacga taataatgtc ttcgttcacc tcgtgttttt gcaatacgag atgcagatcg
      361 ccaatcgcgc cgagcttgtt cgagtcgtcg gtgctgccgt cgttgacgat cgtgaaaccg
      421 aaatgaagag cgggacgctg gtacgtctgg gcccagtttt cgaagtggct tacgaacttc
      481 gcattcgtga caatgtaagc gtggtcgata tcagggatgg tggagatgtt gtccagcaca
      541 tgttccagca tcggcttgcc cgcaaccggc aaaagaggtt tgggctgatt cagtgtcaaa
      601 ggataaagac gcgtggcata tccggccgcc aaaatgatca gtttcatcag atttcttccg
      661 ccccattaga aggctcacaa acatagatct ccgggttcac cgccattgct tgctggtact
      721 cccgcgccac actttgtttg aactgctcca cgtgtacggc gtcaacaata ttaacggtgc
      781 aaccaccaaa tcccccgccg gtcatgcgcg caccgtgaac accttcgact gtattcgcga
      841 gctcgaccat taaatccaat tcgctgctac tcacctcgta atcgtcgcgc agactgcgat
      901 gcgactgggc catcagttca ccaaaacggt gcaggtcgcg cttttcgagt gcttcgccgg
      961 ccgccagcac gcgcgcgttc tcagtgatta cgtgacggca tcgactataa accacgtcag
     1021 gaagatcgcg accgaactct tcgagctgct cgattgtcac gtcgcgtagc gccttcacat
     1081 gcggcagaaa tcgccccaga tggtttacgc ccgcttcgca ttgcgcgcgg cgctcattgt
     1141 aagcgcttgc agccagcgca tgtttcacca tcgtgttgca aataactagg ttcactgtgt
     1201 cgggcagtgg caacaacttg aattcgagtg agcggcaatc aagcaacaac gccatttgcg
     1261 cctgcccaaa cagcgctaca aactggtcca tgatgccgac gcgcgcgccg acaaattcgt
     1321 tctcagcctt ttgacaaaga agagctaatt cgcggcgatc gacgttcagt tccgagacgg
     1381 aagtgagcgc gcaggcagtt gcgacttcga ccgccgccga agaactgagc cccgacccga
     1441 ctggtacttc gccgcggatc tgtagatttg caccacgcag acgatgtccg gcgcgttcaa
     1501 gcataacggc aacgccaaca gcatagtcac tccagtggcg gcccggcgcg ggattcggct
     1561 ggtccagatc aaactcgatc tcgtcgtcaa agttttccga tctaatcgcg agcttccggt
     1621 cgtggcgcgg ccaaacacgc gcgaacaccg agagatcgat cgcggcgggc atgacaaacc
     1681 cgtcgttgta gtcggtgtgt tcgccgatca gattgacgcg tccgggcgcg cgaaaagtac
     1741 gtggagaatt atggatgtgg gtttgtgaca tcaagcacga cccggagtag tggccgcttg
     1801 tctgacggcg gtacgggatg ttcgagcatc cacttcttgc cgttgacagc accgggcctg
     1861 aacaaaagcc cgtcgtcggc aatgaacgcc ataaacgctt cgcgtgtagt cttctccgcg
     1921 gccatccgcg aaaacgtctg ttcggtccgc accatctcct ggagcggcgt caacgcctgc
     1981 aacaacatca aagccaaaaa tatccgtgtc attcgtgttt atccgtggct cctatcgccg
     2041 cttgcaacac gctttccggt gtccagcgat cgatcggcct gacttccgac aatcggccgc
     2101 gactcatcac tgccaaacga tcgcacatgc caaacaactc gggcaaatac gaactgacca
     2161 tcaacaccgc tttgttctgc gccgcacatt cggcaatcgt ttcataaacc tgcgccttgc
     2221 tgccgatgtc gatgccgcgt gtcggttcgt caagcaataa cacatcggca tcctggtgca
     2281 gcaagcgcgc caccaccact ttttgctgat tcccgccaga aagcgacccg gcaggttgtt
     2341 gcac
//
LOCUS       DASTEY010000460         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1262512, whole genome shotgun sequence.
ACCESSION   DASTEY010000460 DASTEY010000000
VERSION     DASTEY010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262512"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFO72_08050"
     CDS             <1..472
                     /locus_tag="VFO72_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9479280.1"
                     /translation="IKDDSELRGVLIFRTKSADEAREWVTADPAVAAGYFAVEMHPWW
                     SEDVMKKTTTPQKMMTAYLAFLVRGDKWTPERTPQTEAIQKGHMANIQRLADMKKLVV
                     AGPFGDNGTLRGIFVFRVNSIEEARELAATDPAVQAGRLALQIHPWVVPEGILP"
     gene            492..1112
                     /locus_tag="VFO72_08055"
     CDS             492..1112
                     /locus_tag="VFO72_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9479281.1"
                     /translation="MYCPSCGSEERQLSQFCRACGTDLRVVRNSLERPDAITASAALA
                     REHIGLAIAEQIRQLRTRKDLEHVAEGVLPSIEKFLESPEERRLRRLRAGVVTSSIGF
                     ALALIAFIAALDKSDFFPFMVPGFVTFMIGIGIVINGLLFTLPRKKLPGDTYDALAQQ
                     ALDSAQNSLRYEPSQPVLTNELGPGAPPQLASITEHTTHHLKPSKS"
     gene            1123..1788
                     /locus_tag="VFO72_08060"
     CDS             1123..1788
                     /locus_tag="VFO72_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9479282.1"
                     /translation="MAVELEGGCRVATMSEGIPRLEGSLISIRVLEFGPGHSPELRNE
                     VLDEVLFSLDSCLLYLDGVPHEIEPQSGIFIPAHQTFRVENPSSKIVRLVSSQTPSKG
                     TQIKKPIVRLADQRAMPTGDRWYRVLIDSEVTQFVGSIPPGRAPDHFHEYEEVLFILR
                     GEGRMWAEARSTPIEAGSCIYLPRGQVHCVENTGTGELRLLGVFYPAGSPAVRYETPT
                     QNP"
     gene            complement(1772..2815)
                     /locus_tag="VFO72_08065"
     CDS             complement(1772..2815)
                     /locus_tag="VFO72_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9479283.1"
                     /translation="MDSASAMIELPSLAPSYLGLLHSGELQRRVAALEGLLESCTVCP
                     RDCLNNRLNNDIAACYSGRLPIVSSYTAHFGEEPPLVGTRGAGNIFFGNCNLRCVYCQ
                     NYQISQTHKQQLKNEITHERLAEMMLELQSRGCHNINFVSPTHFAPQMARSILLAAQK
                     GLHLPIVYNTNAYDAVSVLRLLDGVVDVYLPDLKYADDESGYLYSKVSGYKEFSRLAL
                     KEMFRQTGDELVFDERGLLQRGLVIRLLVLPNDIGGVRESIEWIREELSPRVAISLMA
                     QYYPTHQAETNKRYVLLSRRIRESEWVKATTALEQLGMEEGWVQEFDGTAYYYRPDFN
                     DAERPFSDIRDFE"
     gene            2860..>3069
                     /locus_tag="VFO72_08070"
     CDS             2860..>3069
                     /locus_tag="VFO72_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9479284.1"
                     /translation="MRIWLSKNSEVPIREQLVTQIVLGIVSNDLKVNERLPSTRDLAR
                     RYDIHANTVSAAYRELARRGWVEFRK"
BASE COUNT          669 a          853 c          872 g          675 t
ORIGIN      
        1 gatcaaggac gactcagagc ttcgcggcgt tttgatcttt cgcacgaagt ccgccgacga
       61 ggctcgtgag tgggtgacgg cagacccggc ggttgcggcc ggatatttcg cggttgagat
      121 gcatccgtgg tggtcggaag atgtgatgaa gaaaaccacc acgccgcaga agatgatgac
      181 ggcgtatctg gcatttctcg tccgcggcga caaatggaca cccgagcgga cgccgcagac
      241 cgaggccatt cagaaagggc acatggcgaa catccaacgc cttgccgata tgaagaagct
      301 cgtcgttgcc ggaccgtttg gtgacaacgg cacactgcgc ggcatcttcg tctttcgcgt
      361 gaattcgatc gaggaagcgc gcgagctggc ggcgaccgat ccggccgtac aagcgggacg
      421 tttagcgtta cagattcatc cgtgggtggt tccggaagga attttgccgt gagtgttgga
      481 ggcatttaga aatgtactgt ccgtcctgcg gttctgaaga acgacaactc agccagttct
      541 gtcgtgcgtg tggcacggac ctgcgtgtgg tgcgtaacag cctcgagcgg ccggacgcga
      601 tcaccgcgtc cgcggcgttg gcgcgtgaac acatcggttt ggcaatcgcc gagcagatca
      661 ggcaattgcg tacgcgcaaa gacctcgagc acgtggcgga aggtgtcttg ccgtcaatcg
      721 agaagtttct ggagtcgccg gaagagaggc gtttgcgccg gctgcgcgcg ggagtggtta
      781 cttcctcgat cggctttgcg ctcgcactga ttgcttttat agcggcactc gacaagagcg
      841 atttctttcc gtttatggtg ccgggtttcg tcacgttcat gattggcatc ggcatcgtca
      901 tcaacggtct gctgtttacg ctgccgcgta agaagttgcc tggcgatact tacgatgcgc
      961 tcgcgcaaca ggcgctcgac tccgcgcaga acagtttgcg atacgaaccg tcccagcctg
     1021 ttcttaccaa cgagcttgga ccgggcgcac ctcctcaact tgcctcgatc acggaacaca
     1081 ccactcatca cctcaagccc agcaagtcct aaggagcgcc ggatggctgt cgagcttgag
     1141 ggaggttgcc gcgtcgcaac gatgagcgaa gggattccgc gactggaagg gtcgctgatc
     1201 tcgatacgag tactcgagtt tgggccaggc cactcaccgg agttacgaaa cgaagtcctc
     1261 gacgaggtgc ttttttcgct ggatagttgt ttgctctatc tcgacggcgt tccccacgag
     1321 atcgaacccc aatcaggtat ttttatcccg gcccaccaga cgtttcgcgt cgagaacccc
     1381 agttcaaaga tagttcgtct cgtgagttcg caaactccgt ctaagggaac acagataaaa
     1441 aagccaatcg taagactcgc ggatcagcgc gcgatgccga ccggtgatcg ttggtatcgc
     1501 gtgttgatcg actctgaggt gacgcagttt gtaggttcga ttccgccggg tcgcgcgccg
     1561 gaccactttc acgagtacga agaggtgtta tttattcttc gaggcgaagg acgaatgtgg
     1621 gccgaagcca gatctacacc gattgaagcc ggatcttgta tatacctacc acgggggcaa
     1681 gtgcactgtg tggagaatac aggcacgggc gagttgcgtt tgcttggtgt gttctatccg
     1741 gccggcagtc ccgccgtcag gtacgaaaca cctactcaaa atccctgatg tcactaaacg
     1801 gtcgctcggc atcgttgaaa tcggggcggt agtaataggc agttccgtca aactcctgca
     1861 cccaaccttc ttccatccct aactgctcga gcgcagttgt tgccttgacc cattccgatt
     1921 ccctgatgcg gcgcgaaagc aacacgtaac gcttattcgt ctccgcctgg tgcgttgggt
     1981 agtactgcgc catcagcgag atcgcgactc gcggactcaa ctcttctctg atccactcaa
     2041 tcgactcccg cacaccgcca atatcattcg gcaacacgag taacctgatc actaatccgc
     2101 gttgtaacag cccgcgttca tcgaaaacca gttcatcacc cgtttgccgg aacatctcct
     2161 tcagcgcgag ccgcgaaaac tctttgtatc cacttacttt cgaatacaaa taacctgatt
     2221 catcatccgc gtatttcaag tccggcagat agacgtcgac gaccccatcg agcagtcgca
     2281 gcacggaaac cgcatcgtac gcattcgtgt tgtaaacaat cggcagatgc aaacccttct
     2341 gtgcggccaa cagaatcgac cgcgccatct gcggcgcgaa gtgtgtcggc gaaacaaagt
     2401 tgatgttgtg gcaaccgcga gactgcaact ccagcatcat ctccgcgagc cgttcgtgag
     2461 taatttcatt cttgagttgc tgcttgtgtg tttgcgaaat ctggtaattt tggcaataga
     2521 cgcagcgcag attgcagttg ccgaagaaga tattgccggc cccgcgtgtg ccgaccagcg
     2581 gtggttcctc accaaagtgc gccgtgtaag acgaaacgat gggcaaccgg cctgagtaac
     2641 acgcagcgat gtcgttgttg agtcgattat taagacagtc gcgcgggcag acggtgcaac
     2701 tttccagcag gccttcgagt gctgccacgc gccgctgcaa ctcgccgctg tgcagcaagc
     2761 cgagatacga tggcgccaag gagggcaact caatcatcgc ggaagcggaa tccatcgttg
     2821 ctcttttata gttaatgctg taatcctgtc aaaacttaca tgcgcatctg gctctccaag
     2881 aacagcgaag tgccgattcg cgagcagttg gtgacgcaaa ttgtcctcgg catagtcagt
     2941 aacgatctga aggtcaacga gcgcctgccg agcacgcgcg acctcgcgcg ccgctacgat
     3001 attcatgcca acacggtcag cgcggcgtat cgtgagcttg ccaggcgtgg ttgggtggag
     3061 tttcggaag
//
LOCUS       DASTEY010000461         5249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_521305, whole genome shotgun sequence.
ACCESSION   DASTEY010000461 DASTEY010000000
VERSION     DASTEY010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521305"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1043)
                     /locus_tag="VFO72_08075"
     CDS             complement(48..1043)
                     /locus_tag="VFO72_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479285.1"
                     /translation="MKRVLVILIGLLIWSGEVRPQAIDQDDVIRVESELTNLPFTVTD
                     KQRRFVTTLRAEDVRVLEDGVPQTLFTFQRETDRPLAIAFLIDVSGSQERTLPDEKAA
                     ARLFIENVIKSNRDQVAIVPFTGLAYLEQELTRDVLRVYNVLQSVEVAGPAYLGSGRP
                     LSGIATGPGLPAPPPGGSTAIWDAVALTANNILTKAPGLRRRAIILLTDGIDTTSRLW
                     LRDAVNAALAAETVVYSIGIGDGKYGGVDRGPLRDIAERTGGRAFFPGKKTDLNAAFA
                     AIEQELRTQYLVAYSSTNKKRDGAYRKITIEITNPDLRKEKLELRHRPGYFAKPE"
     gene            complement(1056..2378)
                     /locus_tag="VFO72_08080"
     CDS             complement(1056..2378)
                     /locus_tag="VFO72_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9479286.1"
                     /translation="MRKSSIVLPVFLLILVGAVQAQTDAHWPQWRGPFFNGMARGDAP
                     AVWSDTKNIKWKAEIPGRGFSTPVMWGDRIFLTTAIPTGKAETPQPAPAAGRRAGGGA
                     GPLVEHRFEVLCLDRKTGKILWQRTAKTATPHEGYHRAYGSFASNSPITDGKYVYAFF
                     GSRGLYAYDFNGKLIWEKDFGVQMKMRLAFGEGAAPLLLRDQLFVVFDHEGGSFIVAV
                     DKRDGKELWRSARDESSSWSTPLAIEHNGRTEIVVSATNKVRSYDARSGKILWESAGL
                     GSNVIPVPVTHNGMVFVMSGHRDPKLMAIKLGKEGDLTGSDAIAWSHTRGLAYTTSPV
                     LYDNKLYVVTDSGMVSAFNATTGEPYYAQTRLPKASNLKASPVGANGKLYLATEDGDV
                     VVLKMGEKFEVLSVNTLEDQVFIATPVIADGELYLRGQNTLFCISEKK"
     gene            complement(2568..3176)
                     /locus_tag="VFO72_08085"
     CDS             complement(2568..3176)
                     /locus_tag="VFO72_08085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9479287.1"
                     /translation="MKRGPIVYVALGDSTGVGVGAVEGGYVMRLLKRILEHRPGSELM
                     NLCVSGATSADVLHGQLDKGVAREPELVTLGIGINDIGHGVSLGRFAENYDRILSTLK
                     EKTQATIVVTNIPDISSAPQVPSSMRHQYQQQIITFNERLEEIANRHGVTIFDIYSIT
                     TRELPSHPEYFSRDGFHPSDEGYELWANEMWPTIARAIDAAE"
     gene            3309..3668
                     /locus_tag="VFO72_08090"
     CDS             3309..3668
                     /locus_tag="VFO72_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479288.1"
                     /translation="MPQYEPEAWRDQSDASPTAREQCRLLDLLGRINYRILSGNRVYV
                     EVEGVVYKTLYGGYWRSVFTGIEHYLLRCEKVSSYAWADDNSHPIDLPIAYSSGGWTT
                     SGHSLSFLLNRSTEYSE"
     gene            complement(3636..4865)
                     /locus_tag="VFO72_08095"
     CDS             complement(3636..4865)
                     /locus_tag="VFO72_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9479289.1"
                     /translation="MTQTKKELIASRRRLLGRNLSIAYHDPVKIVRGELQYLYDDEGR
                     RYLDAYNNVAHVGHCHPRVVEAGVTQIRTLNTNTRYLHDLIIRYAEQLTATLPEPLRV
                     CYFVNSGSEANELALRLARTHTNAQDMIVLDLAYHGNTTTLIDLSPYKHDGPGGKGAP
                     SWVHKVPWDNAQRVVEAVAQLRPEGRGLCGFIAESMPSVAGQIVLPADYLEKAYAAVR
                     DAGGVCIADEVQTGYGRIGTHFWAFEQYGVVPDIVVLGKPIGNGHPIGAVITTREIAQ
                     SFANGMEFFSTFGGNTVSCAIGLTVLQVVQEEKLQTHALAVGNHLIEGLRELQQRHEI
                     IREVRGSGLFIGVELVRNDQPATSEAGAIVNRMREEGILLGTDGQHRNVLKIRPPMPF
                     SDADADFLLAVLGRAIQ"
     gene            complement(4871..>5249)
                     /locus_tag="VFO72_08100"
     CDS             complement(4871..>5249)
                     /locus_tag="VFO72_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9479290.1"
                     /translation="LRKSVIHGDPNDYNVLVETERVVGLIDFGDAVYSYTVGDLAIAV
                     AYVVLGKTDPFAAAAPVIEGYREEFQLTQDELDALWPLVRLRLCMSVCIAAYQQAQQP
                     ENEYLGISQRLIEETLPRLLATD"
BASE COUNT         1134 a         1538 c         1378 g         1199 t
ORIGIN      
        1 aggaaagagc tttcgcaaat caaactagta aacggcttcg gcgtatttca ttcgggcttc
       61 gcgaagtaac cggggcggtg gcggagttcg agcttttctt tgcggagatc ggggttggtg
      121 atttcgatgg tgatcttgcg ataggcgccg tcgcgctttt tgttcgtcga tgaataggcg
      181 acgaggtatt gcgtgcgcaa ctcctgttcg atcgccgcga acgcagcgtt caaatcagtc
      241 ttcttaccgg gaaagaaagc gcgtcctcca gtgcgttccg cgatgtcgcg caacggacca
      301 cgatccacgc cgccatactt gccatcgccg atacctatcg agtagacgac agtctcggcc
      361 gccaacgcgg cattcactgc gtctcttagc cacaaccggc tggtcgtatc gataccgtca
      421 gtcaacaaaa tgatcgcacg gcggcgtaaa cccggcgcct tagtcaaaat attgttcgcg
      481 gtcaacgcta ccgcgtccca aatcgcagtc gaccctccag gcggcggcgc cggcaaacct
      541 ggaccagtcg cgattccact caacgggcgg ccggaaccaa gataagcagg cccggcaact
      601 tccacgcttt gcaacacgtt gtagaccctc aacacgtcgc gagtcagttc ctgttcgaga
      661 taggccaacc cggtgaatgg aacgatcgcg acctgatcgc gattcgactt gatcacattt
      721 tcgataaaca atctcgccgc cgccttttca tccggcaatg tcctttcctg cgatccactc
      781 acgtcgatca gaaacgcaat cgccaacggc cgatctgttt cacgttgaaa ggtaaagagt
      841 gtttggggca ctccgtcctc gagcacacgc acatcttcgg ctcgaagcgt ggtaacaaag
      901 cgccgctgtt tgtcggtcac cgtaaacgga aggttggtaa gttccgattc gacccggatc
      961 acatcgtcct ggtcaatagc ctgcggacga acttccccgc tccagatcag caaaccgatc
     1021 aaaataacca gaactctctt catagtttgg ccaaactact tcttctcact aatacagaaa
     1081 agcgtgttct gtccccgcag ataaagctct ccatcggcaa tgacgggcgt ggcgataaaa
     1141 acctggtctt cgagcgtatt gacactcagc acttcaaatt tctcacccat cttcaacacg
     1201 accacgtctc catcttcagt tgccagatac agttttccgt ttgcgccaac cggcgaggct
     1261 ttcaggttag acgccttcgg caaacgcgtc tgcgcgtagt acggctcgcc cgtggtggca
     1321 ttgaatgcgc ttaccattcc gctatccgtg acgacgtaga gtttgttgtc gtaaagcacg
     1381 ggtgaggttg tatacgccag gccgcgcgtg tgactccacg caatcgcatc cgatccagtc
     1441 aggtcacctt ctttgccgag tttgatggcc atgagttttg gatcgcggtg gccactcatg
     1501 acgaagacca tgccgttgtg agtaacagga accgggatga cgtttgagcc taaacccgca
     1561 gattcccaaa gaattttccc ggaccgcgcg tcgtaacttc gcactttgtt ggtcgcgctg
     1621 acgacgattt cagttcgccc gttgtgctcg attgcgagtg gcgtactcca cgaactcgat
     1681 tcgtcgcgcg cagagcgcca caactctttt ccatcgcgct tgtcgaccgc aacaatgaac
     1741 gacccacctt cgtgatcgaa aaccacaaac agttgatcgc gcagcaacaa cggcgcggcg
     1801 ccttcgccaa aggccagccg catcttcatc tgcaccccga aatctttttc ccagatcagt
     1861 ttgccgttga agtcgtacgc atacaggccg cgcgagccga agaatgcata aacgtacttg
     1921 ccgtcagtaa tcggagagtt cgaagcaaag ctgccgtagg cgcgatggta accttcgtgc
     1981 ggtgtcgccg ttttcgctgt cctttgccag agaatcttgc cggttttccg atcgaggcag
     2041 agcacttcga aacgatgctc gactaacgga ccagcaccac cgccggctcg acgtcctgct
     2101 gcgggcgcgg gctgtggcgt ttcggccttg ccggttggaa ttgctgttgt gagaaagatg
     2161 cgatcgcccc acataaccgg cgtagaaaaa ccgcgtccgg ggatctcggc tttccacttg
     2221 atgttcttgg tatcgctcca aacagcaggc gcatctccac gagccatgcc gttgaagaat
     2281 ggcccacgcc actgcggcca gtgcgcgtct gtttgcgcct gaacagctcc aaccaggatt
     2341 agcaagaaca ccggcaaaac aatcgaactc ttcctcattc agcttttcct cctaaattaa
     2401 atgcttattc cggcaggtta gtgatgcagt tgaaattacg gaccgtttac ctgggaccgc
     2461 acgcgtctcg cttgcatctc acggcagcgc ccacttggtc accgtgcagg cgagacgcct
     2521 gcggtcccag gagggaggcg cccaggtttg caaactgcat tcattgtcta ctctgcggcg
     2581 tcgattgcgc gcgcaatggt aggccacatt tcgtttgccc acaactcgta accttcgtcc
     2641 gaaggatgaa aaccgtcacg cgagaagtat tccggatgag acggcagctc gcgagttgtg
     2701 attgagtaaa tgtcgaaaat cgttacgccg tggcgatttg cgatctcttc gagtcgctcg
     2761 ttaaaggtga tgatctgctg ctggtactgg tggcgcatcg acgacggtac ttgcggcgca
     2821 ctcgagatat cgggaatgtt tgtcactacg atcgtcgcct gcgttttctc tttcagcgtg
     2881 ctcagaatgc gatcgtagtt ctcggcaaac cgtccgagcg acacgccgtg tccgatgtcg
     2941 tttatgccga tgccaagcgt aacgagctcc ggctctcttg cgacgccttt gtccaactgg
     3001 ccgtgcaaaa cgtcggcact ggttgcgccg ctgacgcaga gattcattag ttccgatcct
     3061 gggcggtgtt cgagaatgcg tttcaagagc cgcataacgt aaccaccttc aaccgcgccc
     3121 actcccacgc cggtcgaatc tccgagcgcc acgtaaacga tgggacctcg cttcatttca
     3181 accttacccg tgaccgttcc ctcttgcgtt ctctgactgc aagcgacaaa caaaagtaac
     3241 agagcgaaag gaagtagctg tcgcaaattc ggtgacatcc gtgtaaaccc tggattaaac
     3301 tatccgccat gccgcagtat gaaccggaag catggcgcga tcaatcggac gccagcccaa
     3361 cggcgcgaga acaatgtcgc ctgctggatc tgctgggacg aattaactat cgcatcctga
     3421 gcggtaatcg cgtgtatgtt gaggttgagg gagtggttta caaaacgctg tacggcggtt
     3481 actggcgcag tgtcttcacc ggaatcgagc attacctttt gcgctgcgaa aaggtttcca
     3541 gttacgcctg ggctgatgac aattcacacc cgatcgacct gccgatcgcc tactcaagcg
     3601 gcggttggac caccagcgga cattcgcttt cgttcctact gaatcgctcg accgagtaca
     3661 gcgagtagaa agtctgcatc cgcgtctgag aacggcatcg gcggccggat cttcagaacg
     3721 tttcgatgct gaccgtccgt gccaagcaag attccttctt cacgcattcg attcacaatc
     3781 gcgccggcct cgctggtcgc cggttgatcg tttcttacca gttcgacacc gatgaacaga
     3841 cccgagccgc ggacttcacg gattatttcg tgacgttgct gtagctcgcg cagaccttcg
     3901 atgaggtggt ttcccacggc gagcgcgtgt gtctgaagtt tctcttcctg gaccacttgc
     3961 aaaaccgtca aaccgatcgc acaggagact gtgttgccgc cgaaggtgct gaaaaactcc
     4021 atgccgttag cgaacgactg tgcgatttca cgtgtggtga tcacggcgcc gatcggatgt
     4081 ccattgccga tcggtttgcc gagcacgaca atgtcgggaa caacgccgta ctgttcgaac
     4141 gcccagaaat gcgtgccaat gcggccgtag ccagtctgca cttcatcggc gatgcagacg
     4201 ccaccagcat ctctgaccgc tgcataggct ttttccagat aatcggccgg caggacgatc
     4261 tgccccgcaa cactcggcat cgactccgct atgaatccac acaaacctct gccttcaggt
     4321 cgcaactgag caaccgcttc gactacgcgt tgcgcgttgt cccagggcac cttgtgcacc
     4381 cacgagggcg ctccttttcc tcctggtcca tcgtgtttgt aagggctgag gtctatcagc
     4441 gtagtcgtgt ttccgtgata ggcgagatcg agcacgatca tgtcctgagc gttcgtgtgt
     4501 gttcgagcga gtcgcagcgc gagttcgttg gcttcgctgc cggaattgac gaagtagcag
     4561 acgcggagtg gttcgggcaa cgtggccgtt agttgttcgg cataacgaat gatcaggtca
     4621 tgcaggtagc gcgtgtttgt attcagcgtg cggatctgcg ttacgcccgc ttcgacaacg
     4681 cgcggatgac aatgaccgac gtgcgcaacg ttgttgtaag cgtcgagata gcggcgtccc
     4741 tcatcgtcgt agagatactg caactcgcca cgcacaatct tcacaggatc gtgatacgcg
     4801 atactcaagt tgcgcccaag caaccggcgt cgtgacgcta tcagctcttt cttagtctgt
     4861 gtcatctgtg tcagtctgta gctaacaacc gcggcagagt ttcttcgatc aatcgctgac
     4921 tgattcccag gtactcgttc tcaggttgtt gcgcctgttg ataggcggca atacaaacac
     4981 tcatgcacag tcgcagccgc accagcggcc ataatgcgtc gagttcatcc tgcgtaagtt
     5041 gaaactcctc tcggtagccc tcgatcacag gagcagccgc ggcgaacgga tctgtcttgc
     5101 ccaacaccac gtacgcgacc gcgatcgcca gatctccgac cgtgtagcta taaactgcat
     5161 ctccaaaatc gatcagccca accacccttt ccgtctcaac caaaacgttg taatcgttcg
     5221 ggtcgccgtg aatcacactt ttccgcaaa
//
LOCUS       DASTEY010000462         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_801409, whole genome shotgun sequence.
ACCESSION   DASTEY010000462 DASTEY010000000
VERSION     DASTEY010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801409"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFO72_08105"
     CDS             complement(<1..431)
                     /locus_tag="VFO72_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9479291.1"
                     /translation="MSDSTATPTKEELAVLSWFERFAYRLVRRMNRGAWKRFWTWCQS
                     TMGAGWIHLSTYNIMNVYGLENIEAASRERPLLLVANHRSFFDMYAVSTVLFRTTKWR
                     KQLFFPVRGRFFYQSPLGLFLNLIMGWWSVYPPFFATGDHP"
     gene            complement(428..3937)
                     /gene="metH"
                     /locus_tag="VFO72_08110"
     CDS             complement(428..3937)
                     /gene="metH"
                     /locus_tag="VFO72_08110"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET9479292.1"
                     /translation="MSNLLDAIKERIVVFDGAMGTNLQVQNLTLDDFGGPRFEGCNEN
                     LLVTRPDAVEKVHAGFLAVGCDVIETNSFNGTPVDFAEYGIADQAYDMNVRAARLAKR
                     IASDYSTKAKPRWVAGSMGPGRKLPTLGHISFVELRDAYRVQARGLLDGGADLMIVET
                     CQDLLQTKAALSAIFSVFEETHTRVPVIAQVTIEVFGTMLNGTEIAAALTALAPFPID
                     VIGMNCGTGPKHMTESIRYLCENAPLPVSALPNAGLPSVVEGKMHYDLTPETFTAQLV
                     HFAGDFGVNIVGGCCGTTPEHLRLVVEAMQRITPRQRNPKLVPAASSIYIQQPYVQDA
                     SFLIIGERVNASGSKKMRDLLNAGDWDGLVALGKDQEREGAHILDVNVDFVGRDGESD
                     MHELASRLVTNVKIPLMFDSTEWQKMEAGLQHAGGKSILNSTNYEDGVPRFLKVIDLA
                     KRYGASVVIGTIDEEGMARTAEGKIKIAKRAYEQATREAGLPASDVFFDPLALPISTG
                     IEEDRRNALETIEAIKRIKSELPGAYTLLGVSNVSFGLNPASRVVLNSVFLHDAVEAG
                     LDAAIVNASKIEPLNRIGEKETRVARELIYDQRRFENDVCVYDPLTEFTKLFEGVKSK
                     ATKKASKGETVEERLKNHIIDGEKIGLEDELKVALEKHPPLDIINNILLDGMKVVGDL
                     FGSGQMQLPFVLQSAEAMKAAVRFLEPFMEKKGGATAKGTMVLATVKGDVHDIGKNLV
                     DIILTNNGYKVHNLGIKQPIEAILQAYDETGADAIGMSGLLVKSTLIMKDNLELMNER
                     GVKVPVILGGAALTRRYVEEDLKSLYKGQLFYAHDAFAGLRTMDQLVGATDQTDNTDR
                     VETVAVPEDVEDLVGEEAKLGVRKTARPRGAEPAGNTAHTSRSDVNPEAPIPTPPFYG
                     SRVVEDVLLDEVFAYVNENALFKGQWQFKQGKTPPEEYQALVRDKVRPIYEELKERSK
                     LQKLLVPKVVYGYFNCQSAGNNLIIYDQNQREQVRFTFPRQPAGKHLCLADFFASQES
                     GRMDVVAFHLVTVGRRASEYSHELFTSDKYADYLYFHGLSVETAEALAEFWHKRIREE
                     LGIAGSDASNLLRLFHQQYQGSRYSFGYPACPRLEDQEKLFTLLDPGRIDVALTEEFQ
                     LEPEQSTSAIIVHHPEAKYFSID"
     regulatory      complement(4128..4203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            4294..4788
                     /gene="coaD"
                     /locus_tag="VFO72_08115"
     CDS             4294..4788
                     /gene="coaD"
                     /locus_tag="VFO72_08115"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9479293.1"
                     /translation="MRRAIYPGSFDPVTNGHLDIIERGCKLFDEIIIAILINPEKQPF
                     FTVEERHEMLLKVLPQINHGRCNVRVDSFSGLLVEYAVAQQAGVIVRGIRAISDYEYE
                     LQMALMNRRLEPGIETVFMMPAESYSYVSSRLVKEVFQLGGTVEGLVPPLVEQRMKDK
                     IAQR"
     gene            4818..>5734
                     /locus_tag="VFO72_08120"
     CDS             4818..>5734
                     /locus_tag="VFO72_08120"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9479294.1"
                     /translation="MSSFSVSENVARMQSSATMAAMQAAEALRAAGVNVCDFGAGEPD
                     FDTPDNIKQAATAAMKAGRTKYTSAGGIREIQSAIIDFYQREFGTEYQPTEVMTTAGG
                     KQAIFNAVVTLINPGDEVLIPKPYWVTFPEVVVFAGGTPVYIETEETDFVLTVEQVQQ
                     AITPRTKLLILNSPSNPSGRVLPPNEFRRILELTTERGIYVVSDECYLRFVYPPAEVF
                     SAASLPAELRSRLCIAGSFSKTYAMTGWRVGYALAPAEWTKAMVKVQGHSTSNTNSIA
                     QWAAVEALSGPQESVSTMLAEYTKRREWLL"
BASE COUNT         1241 a         1655 c         1546 g         1292 t
ORIGIN      
        1 atcggatgat cgccagtcgc aaaaaacggc ggatacacgg accaccagcc catgatcaga
       61 tttaaaaaca gtcccagcgg cgactggtaa aaaaatcttc cgcgcacggg aaagaagagt
      121 tgtttgcgcc acttggtcgt gcgaaatagc acagtcgata cggcgtacat atcgaagaag
      181 gagcgatggt tggccaccag cagcagcggc cgctcgcgcg acgcagcctc gatgttttcg
      241 aggccgtaaa cattcatgat gttgtaggtg gagaggtgaa tccagcctgc gcccatcgtg
      301 ctttgacacc acgtccagaa ccgcttccag gccccgcgat tcattcgccg aacgagacga
      361 taggcaaacc gctcaaacca cgaaagtacg gcgagctctt ctttggttgg agttgcggtt
      421 gagtcgctca atcgatcgaa aagtacttcg cctccggatg gtgcacaata atagctgaag
      481 tcgactgttc cggctccaac tgaaactctt ccgtcagtgc aacatcaatc cggcccggat
      541 cgagcagcgt aaacagcttt tcctgatctt cgagccgcgg gcaggcagga tagccaaaac
      601 tgtaacgcga accttggtac tgctgatgaa acaggcgtaa caagttactc gcgtcgctgc
      661 cggcgatgcc cagttcttct ctgatacgtt tgtgccaaaa ttccgccagc gcctcggcag
      721 tctcgacgct cagaccatga aagtacaaat agtcggcgta tttgtctgac gtgaacaact
      781 cgtgcgagta ctcactcgcg cggcggccga cggtcaccag gtgaaacgcg acaacgtcca
      841 ttcttcccga ctcctgcgag gcaaagaagt cggccagaca caggtgcttg ccggcgggct
      901 ggcgtggaaa agtgaagcgc acctgctcgc gctggttctg atcgtagatg atcagattgt
      961 ttccggcaga ctggcaattg aagtatccgt agacaacttt gggaaccaac aacttctgaa
     1021 gcttcgatcg ctccttcagc tcttcataga tcggtcgcac tttatctcgc accagcgcct
     1081 ggtattcctc cggcggcgtc ttgccctgtt tgaactgcca ctgccctttg aacagcgcgt
     1141 tctcgttcac gtaagcgaac acttcgtcaa gcaagacgtc ttcaacgaca cgcgaaccgt
     1201 aaaacggcgg tgtagggatc ggcgcctcag gattgacatc cgaacgactg gtgtgcgccg
     1261 tatttccagc gggttcggct ccacgcggcc gcgccgtctt acgcacgccc agtttcgctt
     1321 cctcaccaac gagatcttca acatcctctg gaactgcaac tgtttcaact ctgtccgtgt
     1381 tatccgtctg atccgtggct cccactaact gatccatcgt gcgcaaaccg gcaaacgcat
     1441 cgtgcgcgta aaacaactga cccttataaa gcgacttcag atcctcttcg acgtaacgcc
     1501 tcgtcagtgc cgcgccgccg agaatcacgg gcaccttcac accacgctcg ttcatcagct
     1561 ccaggttgtc tttcatgatc agcgtcgact tcaccagcaa cccgctcata ccgatcgcat
     1621 ccgcgccggt ctcgtcatac gcctgaagga tcgcctcaat cggctgcttg atgccgaggt
     1681 tgtgcacctt gtaaccgttg ttcgtgagaa tgatgtcgac gaggttcttg ccaatatcgt
     1741 gaacgtcgcc cttcaccgtc gcgagcacca ttgtgccttt cgccgtggcg ccgcccttct
     1801 tctccatgaa cggctcgaga aaacggacgg cagccttcat cgcttccgcc gactgcaata
     1861 cgaacggcaa ctgcatctgg ccgctgccga aaagatcgcc gacgaccttc atgccgtcga
     1921 gaaggatgtt gtttatgatg tcgagcggcg gatgtttttc gagcgccact tttagttcgt
     1981 cttcgagccc gatcttttca ccgtcgatga tgtggttctt cagacgttct tcgaccgttt
     2041 cgcctttgga cgctttcttc gtggccttcg acttaacgcc ttcgaatagt ttcgtgaact
     2101 cggtcagcgg atcgtagacg caaacgtcgt tttcaaacct ccgctggtcg tagatcaact
     2161 cgcgcgccac tcgcgtttcc ttttcgccga tgcggttcag cggctcgatc ttgcttgcgt
     2221 tgacgatcgc cgcgtcgagt ccggcttcga cggcgtcgtg gagaaacacg gaattaagca
     2281 ccacgcgtga ggcgggattc aaaccaaacg agacgttcga cacaccgagc agcgtgtaag
     2341 cgccgggcag ttcgctcttg attcgtttga tcgcttcaat agtttccagt gcattgcggc
     2401 ggtcttcctc gataccggtc gagattggca gcgcaagcgg atcgaagaag acgtcagacg
     2461 cgggcaaacc ggcctcacgc gtagcttgct cgtaggcgcg cttcgcaatc ttgattttac
     2521 cttcggcagt gcgcgccatt ccctcttcgt caatagttcc gatcacgacg ctggcgccgt
     2581 agcgcttcgc gagatcgatc accttgagaa agcgtggcac gccgtcttcg tagttcgttg
     2641 agttgaggat cgacttgccg cccgcgtgtt gcagtcccgc ttccatcttc tgccactcgg
     2701 tcgagtcgaa catcaacgga atctttacgt ttgtcaccaa acgcgaggcg agttcgtgca
     2761 tgtcgctttc gccgtcgcga ccaacgaaat cgacgttgac gtcgagaata tgcgcgcctt
     2821 cgcgctcctg atccttgccg agtgcaacca gaccatccca atctcccgca ttcaacaggt
     2881 cgcgcatctt cttcgagccg ctcgcattta cgcgctcgcc gatgatcaga aacgatgcgt
     2941 cctgcacgta aggttgttga atgtagatcg acgacgctgc cggaacgagc ttgggattgc
     3001 gctgcctggg tgtgattctt tgcatcgctt cgacgacgag gcgcagatgc tccggcgtcg
     3061 taccgcaaca gccgccgacg atgttgacgc caaagtcgcc ggcgaagtgg accagttggg
     3121 cggtgaaagt ctcgggcgtt aggtcgtagt gcatcttgcc ttcgacgact gacggcagac
     3181 cggcatttgg cagcgccgaa acaggcaacg gcgcattctc gcagagatag cgaatgcttt
     3241 cggtcatgtg tttcgggccg gtgccgcagt tcatgccgat cacgtcgatc ggaaatggcg
     3301 caagagcagt caacgccgcg gcgatctcag tgccgttaag cattgtcccg aaaacttcga
     3361 ttgtgacctg cgcgattacc ggaacgcgcg tgtgagtctc ttcaaacacc gagaagattg
     3421 ccgagagtgc tgcttttgtt tgcagcagat cctggcaggt ttcgacgatc attagatcgg
     3481 cgccgccgtc gagcagcccg cgcgcctgta cgcggtaagc gtcgcggagt tcgacgaatg
     3541 agatatggcc gagtgtgggg agtttccttc ccggtcccat ggaaccggcg acccagcgtg
     3601 gttttgcttt ggttgagtag tcggacgcga tgcgtttggc aagacgcgcc gcgcgaacgt
     3661 tcatgtcgta agcctgatcg gcgatgccgt attcggcaaa gtccacgggc gtgccgttga
     3721 aggagttggt ttcgatgacg tcgcagccca cggcgagaaa accggcgtgg accttctcga
     3781 cggcatcggg ccgggtcacg agcaggtttt cgttgcaacc ttcaaaacgt ggcccaccaa
     3841 aatcgtccag cgtcaggttc tggacctgaa ggttggtccc catcgcgcca tcgaagacga
     3901 cgatacgttc ttttattgcg tctagtaggt tggacatttc gatgccgatt ttcgattgcc
     3961 gcttgccgct tgctgatttt tgatctttga atttagattt tagattggcc gtcactgctt
     4021 tatctgccga cctgtgtcaa ggacgaggtc ggtaatggac aatcggcaat cggcaatcaa
     4081 aaatcttcaa tgacaaaagg ccccgcgcct aacaacgcgg agccttgaaa actgactcat
     4141 cgagctgttt agcggattat tttacgtggc cgcaagtcgc tttaactcac cacgtcaatt
     4201 tgtcaaagcg tattgaagca tatctcgtca gtcctgtaaa tcctgtccag acatgattac
     4261 aaaatctaca agattcctgt accatgcgcc tacatgcgac gcgccatcta ccccggttcg
     4321 tttgatcccg taactaacgg ccacctggac atcatcgagc gcggctgcaa actgtttgac
     4381 gaaatcataa tcgccatcct cattaacccc gaaaagcagc cgtttttcac tgtcgaagaa
     4441 cgtcacgaga tgctcctcaa ggtcttgcca cagatcaacc acggacgttg taatgtgcgt
     4501 gttgattcgt tcagtggact gctggtggaa tacgccgtcg cacaacaagc cggcgtgatc
     4561 gtgcgcggca tacgcgccat ctccgattac gaatacgaac tgcaaatggc gctgatgaat
     4621 cgccgcctcg agccgggcat agagactgtg ttcatgatgc ccgcggagag ttattcttac
     4681 gtaagctcgc gcctggtcaa agaagtattc cagttaggcg gcacggtcga aggcctggtg
     4741 ccgcccctcg ttgaacaacg tatgaaagac aagatcgccc aacgatgaaa tctctttccg
     4801 ctatatcgaa gcccgctatg tcatcttttt ctgtctctga aaacgtcgcg cgcatgcagt
     4861 cgtcagcaac gatggcagcc atgcaagcgg ccgaagcttt acgcgcagcc ggtgtaaacg
     4921 tttgcgactt cggcgcgggc gaacccgact tcgacacgcc ggacaacatc aagcaggccg
     4981 cgaccgccgc gatgaaagca ggccgcacga agtacacgtc ggccggcggc atccgcgaaa
     5041 ttcaaagcgc catcatcgat ttctatcaac gcgaatttgg tacggagtat caacccaccg
     5101 aggtaatgac gaccgccggc ggcaagcagg cgatcttcaa cgccgtcgtg acgctaatta
     5161 atcctggcga tgaagtcttg attccgaagc cttactgggt gacttttccg gaagtggtcg
     5221 tctttgccgg cggcactccg gtctacatcg aaaccgagga aacagatttt gtgctcacgg
     5281 tcgagcaggt ccaacaagcc atcacgccgc gcacgaagtt actaatctta aactctccgt
     5341 caaacccttc gggacgcgtg ctgccgccaa acgagttccg ccgcattctg gaattgacga
     5401 ccgaacgcgg catctacgtc gtttcggacg agtgttactt gcgcttcgtt tatccgccgg
     5461 cggaagtatt cagtgcggct agtctgcctg cggaactcag atcgcgcttg tgcatcgccg
     5521 ggtcgttctc gaaaacatat gccatgacgg gatggcgagt cgggtatgca cttgcgccgg
     5581 ccgagtggac caaagcaatg gtgaaagtgc agggccattc aacgtcgaac accaactcga
     5641 tcgctcaatg ggccgcagtc gaagcgctaa gcgggccgca ggagtccgtt agcacgatgc
     5701 tcgccgagta caccaaacga agggagtggc tact
//
LOCUS       DASTEY010000463         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1081757, whole genome shotgun sequence.
ACCESSION   DASTEY010000463 DASTEY010000000
VERSION     DASTEY010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081757"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFO72_08125"
     CDS             <1..492
                     /locus_tag="VFO72_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9479295.1"
                     /translation="HAHPPHGTAFAVAGLAIDQPILSEVILTLGCVPLASYGTPSTEE
                     LTEAMQPLIKHHNALLMANHGAVAYGSDLWQAWDRLETLEHTAKIAILSRVLGGSKNL
                     PADAIEKLINVREEAGYLGESARGQACGYLREPLVENGAGKITLTREELVELLSQAVN
                     GNR"
     gene            529..810
                     /gene="eutM"
                     /locus_tag="VFO72_08130"
     CDS             529..810
                     /gene="eutM"
                     /locus_tag="VFO72_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HET9479296.1"
                     /translation="MQEALGMVETKGLVAMIEAADAMVKAANVTLVGYEKIGAGFVTA
                     IVRGDVAAVKAATDAGAAAARRGGELVSVHVIPRPHGSVDETLPIGVDR"
     gene            817..1086
                     /locus_tag="VFO72_08135"
     CDS             817..1086
                     /locus_tag="VFO72_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9479297.1"
                     /translation="MIIARILGTVVSTQKDERLHGKTLLIVRPINVDGSDTTGYVVAV
                     DTVGAGFHERVLVVAGSSARLAQGLKDVPVDAAIVGVIDTVDVQN"
     gene            1196..1471
                     /locus_tag="VFO72_08140"
     CDS             1196..1471
                     /locus_tag="VFO72_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9479298.1"
                     /translation="MQLARVIGTVVATVKNDSLEGRKLLVVQTLDRDLEPIGKPMVAV
                     DAVGAGIGELVFWCRGKEASFPFKREDTPTDCTIVGIVDSEQHVGHK"
     gene            1468..1740
                     /locus_tag="VFO72_08145"
     CDS             1468..1740
                     /locus_tag="VFO72_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HET9479299.1"
                     /translation="MILARVIGNVVATQKNRRYQNARVMLCQQITPEGDETSYSVLAL
                     DSVDAGVGDTVLIVQEGWSASTAATGEAGAAIDSAIVGVVDRIDLL"
     gene            complement(2109..>2359)
                     /locus_tag="VFO72_08150"
     CDS             complement(2109..>2359)
                     /locus_tag="VFO72_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9479300.1"
                     /translation="AYPQLQSNQNFLKLQDEVAGTENRIATSRKDYNDATQDYNTTRA
                     RFPTVISAKLFGFKEEPYFRAEEGAQQTPTINANTLRR"
BASE COUNT          525 a          581 c          741 g          512 t
ORIGIN      
        1 cacgcgcatc cgccgcatgg aacagcattc gcagtggccg gcctggcgat agaccaaccg
       61 atactctcag aagtgatact gacgctaggt tgtgtaccgt tggcgtctta cggaacacct
      121 tcaactgaag agctcacgga agcaatgcag ccgttgatca aacatcacaa cgcactgcta
      181 atggctaacc atggagctgt ggcgtacggg tcagatctgt ggcaggcgtg ggatcgtctg
      241 gaaacattgg aacacacggc caagatcgcg atcttgtcgc gcgtgcttgg tggttcgaag
      301 aatttgccgg ctgatgcgat cgagaagtta atcaacgtgc gcgaagaggc gggttatctc
      361 ggcgagtcgg cgcgcggtca ggcgtgcggg tatttgcgag aacccctggt tgagaatgga
      421 gctggaaaga tcacgctgac acgtgaggaa ctagtagagt tactaagcca ggcggtgaac
      481 ggcaacagat aaacacgaat tttgaactca cataagagga gcagcttaat gcaggaagca
      541 ctcggaatgg ttgaaactaa aggactcgtc gcgatgattg aagcagccga cgcgatggtc
      601 aaagccgcca acgtcactct cgttggttat gaaaagatcg gcgccggctt cgtcactgcg
      661 attgtgcgtg gcgacgttgc ggccgttaaa gcagcgactg acgcgggcgc cgccgccgcg
      721 cgacgtggtg gtgaactggt cagcgtgcac gtgattccgc gtccgcacgg cagcgttgac
      781 gagacccttc cgattggtgt cgatcgatag gcaggcatga tcatcgctcg catcctcggc
      841 actgttgtgt ccacgcaaaa ggacgaacgg ttacacggaa aaacgttact tattgttagg
      901 ccgatcaatg tcgacggcag cgacacgacg ggttatgtgg ttgctgttga taccgtcggc
      961 gccggttttc acgagcgcgt gctggttgtt gccggatcga gcgcacgcct ggcgcaggga
     1021 ttgaaggacg tgccggtgga tgcggcgatc gtgggtgtga tcgatacggt cgacgtacaa
     1081 aattgagctt tgtacttgga actttgttct ttgacgctac tcaatcgcct ggcagtcatc
     1141 ggagcgtgca agtagcgaac aaagtacaaa gcacaaagta caaaaaacca acgaaatgca
     1201 acttgcgcga gttatcggca cagtggtcgc gaccgtcaag aacgattcgc ttgaaggccg
     1261 aaagttgtta gttgtgcaaa cattggacag ggacctcgag ccaattggca agccaatggt
     1321 agctgttgat gccgttggcg ctggaatcgg tgaactcgtt ttctggtgtc gtgggaagga
     1381 agcgagtttt cctttcaaac gcgaagacac accaactgat tgcacgattg taggaatcgt
     1441 cgattctgag cagcacgtcg gacacaaatg attctcgctc gcgtaatcgg aaacgttgtc
     1501 gccacgcaga agaaccgtcg ctaccagaac gcgcgcgtga tgttgtgcca gcagatcacg
     1561 cctgaaggag atgagacgag ctactcggtg ctcgcgctgg actcggtgga tgctggtgtc
     1621 ggcgacacgg tgctaatcgt gcaggaaggc tggagcgcct ccaccgcggc gacgggcgag
     1681 gctggcgcgg cgatcgactc ggcgatcgtc ggggtcgtgg atcggattga tctgttatga
     1741 gttctgacca ggcgagagaa gtagcggaaa ggatcgcacg gcgggtaggc gccgcttcgg
     1801 cgacgccgag cgttgggatt agtgcggagc ttgccgctat gcgtgcgacg ctgaatgaac
     1861 tgcaaaaccg attgatacag atcgagtcga gagttggctc gagtgctact gttgcaccgc
     1921 gggttcagtc accctggctt gccggtgtga acgcgagcca tccgagcatg gaacggtttg
     1981 gcgttgaaga agctactgtt tcggagctgg tagatttttt ccaaaatgaa aaggcttgca
     2041 gcctcgagcc cggcggcaag ccttgcgatc actgtgcgat gtgcagttcg agggggttct
     2101 agactctcct atctccgcag cgtgttggcg ttgattgtgg gcgtctgctg cgcaccttcc
     2161 tccgctctga agtacggctc ctctttaaat ccgaaaagct tcgctgagat cacagtcgga
     2221 aaacgcgcgc gtgtagtgtt gtagtcctgc gtcgcgtcgt tgtaatcctt gcgcgacgtg
     2281 gcgatgcgat tctcagtgcc cgcgacttcg tcctgcaact tcagaaaatt ctggtttgac
     2341 tgtaactgcg gatacgcct
//
LOCUS       DASTEY010000464         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1410886, whole genome shotgun sequence.
ACCESSION   DASTEY010000464 DASTEY010000000
VERSION     DASTEY010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410886"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VFO72_08155"
     CDS             <1..1882
                     /locus_tag="VFO72_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9479301.1"
                     /translation="SRFNKLVFVLDPFGVPGAIPEEVALFSYGETDGGTWAAFHREEE
                     FKKGTASSSEDRRIIDITQHDIEGAIKGSHLTITDRVTFKNLIAGTRVVPFNLFGSLR
                     VTSVLDADGANLDFIQESKDEDSDFGVILTKPLEAGQTHQLVISYDGDGALRDSGGGN
                     FILTQRSTWYPANANTLFGEDRALYNMTFRYPKQYMFVGTGAAVEPDARDGDVSVAKW
                     SSGKTELAVAGFNYGRFKRKEVADKESGYNVEFYANIEVPDELKAIQRNIELAESQGV
                     RTMTTLGSISTTSMADPAIADAQNSMRIYNAFFGKLPYTRIAMTQQPAAGFGQAWPTL
                     VFMPYMAYIDTTQRAQLLGTQGGTDTFWRYVAPHEVAHQWWGHIIGWDSYRDQWMSEG
                     FAEFSASLYVQLTRGNDKFVDFWEDLRQQIVEARPQTRGRKPYTIGPVTQGYRLNNGK
                     TGGAARFLIYPKGAYILHMLRMMMYKQGQGGDAAFQAMMKDFVQTHYNQAVSTEDFKR
                     SVEKHMTKEMDIDKNGKMDWFFDEWVYGTEVPAYKFEYSVSKDGMLNGKLTQSGVSDK
                     FVMLVPIYLDMGKGWVKLGSATMVGSATVDIKDLKLPAVPKRMSVCAMNDVLATSIEN
                     SK"
BASE COUNT          496 a          559 c          577 g          403 t
ORIGIN      
        1 cagccggttc aacaaactag tcttcgtact cgacccgttt ggcgtgcccg gagccattcc
       61 ggaagaggtt gcgctgttta gttacgggga aactgatggg ggcacttggg cggctttcca
      121 tcgcgaagaa gagtttaaga agggcaccgc gtcgagctcg gaagaccgtc gcatcatcga
      181 catcactcag cacgatatcg aaggcgccat caaaggctcg catctcacga ttaccgatcg
      241 cgtcacgttc aagaatctca ttgccggcac acgcgtcgtt ccgttcaatc ttttcggctc
      301 gttgcgcgtg acaagtgtgc tggatgccga cggcgccaat ctggacttca tccaggaaag
      361 caaagacgag gattccgact ttggcgtgat cctaaccaag ccactcgaag caggacaaac
      421 tcaccagcta gtgatctctt acgacggtga cggcgcactg cgcgattccg gcggcggcaa
      481 tttcatcctg actcagcgct cgacgtggta tcccgccaat gccaacacgc tctttggcga
      541 agaccgcgcg ctatacaaca tgacattccg gtatccgaag cagtacatgt ttgtcggcac
      601 cggcgcggcg gtcgagcctg acgcgcgcga cggtgatgtg tcagtcgcca agtggtccag
      661 cggaaaaact gaattggcag ttgccggttt caactacggc cgcttcaaac gcaaggaagt
      721 tgctgacaaa gagagcggtt acaacgtcga gttttatgca aacattgaag tgcccgacga
      781 gttgaaagca attcagcgaa acatcgagct agccgaaagt cagggcgtca gaacgatgac
      841 cacgttgggg tcgatctcca ccaccagcat ggccgacccg gcgatcgccg acgcgcagaa
      901 ttcgatgcgt atttacaacg cgttctttgg caaactcccg tacacacgaa tcgcgatgac
      961 tcaacagccg gccgcagggt tcggacaagc gtggcccacc ttggtgttta tgccctatat
     1021 ggcttacatc gataccaccc aacgcgcgca gttactcggc acgcaagggg gcaccgacac
     1081 tttctggcgc tacgtcgcgc cccacgaagt ggcccatcaa tggtggggac acatcattgg
     1141 ctgggacagt tatcgcgacc agtggatgag cgagggcttt gcggagtttt cagcttcgct
     1201 ctacgtgcag ttaacgcgcg gcaacgacaa gtttgtcgac ttttgggaag acctccgcca
     1261 gcagatcgtg gaggcgaggc cgcaaacaag aggtcgcaag ccttacacga ttggtcctgt
     1321 cacgcagggc tatcggctta acaatggcaa gaccggcggt gcggcgagat ttttgatcta
     1381 tcccaaaggc gcctacatcc tgcacatgct gcgcatgatg atgtacaagc agggccaggg
     1441 cggcgacgcc gcgttccagg cgatgatgaa agacttcgtc caaactcact acaaccaggc
     1501 cgtctcgacc gaagatttta aacgcagcgt cgagaagcac atgacgaaag agatggacat
     1561 cgacaagaac ggcaagatgg actggttctt cgacgagtgg gtttacggca cggaagtccc
     1621 ggcatacaag tttgagtaca gtgtcagcaa agacggtatg ctcaacggga agctcacgca
     1681 gtcaggagtt tccgacaagt tcgtgatgct ggtgccgatc tatctcgaca tgggtaaggg
     1741 ctgggtgaaa cttggctcgg cgacaatggt gggtagcgcg actgtcgaca tcaaagatct
     1801 caaactgccg gcggtaccga aacgcatgtc ggtttgtgcg atgaacgacg tgctcgcgac
     1861 cagcatcgag aatagcaagt aactacccgg cgagaatagt aagtactacc tgggaccgca
     1921 ggcgtcccgc ctgcactgtt acgcaaagca acgttgcgta aacagcagac gagacgcctg
     1981 cggtcccgaa ttttacgcat aacttgcgta gaccgacttc accttatcga taaac
//
LOCUS       DASTEY010000465         4625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_652754, whole genome shotgun sequence.
ACCESSION   DASTEY010000465 DASTEY010000000
VERSION     DASTEY010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652754"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VFO72_08160"
     CDS             complement(<1..1377)
                     /locus_tag="VFO72_08160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9479302.1"
                     /translation="MSFFFAQSVMPIINWWLIAPEMIVCAAAVVVMLVDAFVRPAQRW
                     ITGNISLVGLLLAGAVSIYLWVNGAPSADAFNGMIALDELRLGFTLIFLLVSALTLLL
                     SIVWVEYEQLPAGEFHSLLLFATVGMMLMASGNDLVIIFLGLEILSIATYVLAGFRRT
                     DIRSNESSLKYFILGSFSSAFLLYGIALVYGATSIAEPGPGESLSRIVAGTTNIAEIA
                     SRINQAQYPALLYAGAAMMLVGFGFKIATAPFHIWTPDVYEGAPTPVTAFMAAGPKAA
                     GFASFMRVFIFGLPFVVSASNATAGNLHQVWVTTLVVMAILTMTLGNVVAIVQNNIKR
                     MLAYSSIAHAGYALVGVVAAGAATDVTQRNSAITAVMFYLLTYAVMNIGAFAVVQLIA
                     RSGDRRTSVEDYSGIGFESPVLAFSLSLFMLSLLGMPLTAGFIGKVMVFGAAIDQGYY
                     TLVVIGVLN"
     gene            complement(1495..3039)
                     /locus_tag="VFO72_08165"
     CDS             complement(1495..3039)
                     /locus_tag="VFO72_08165"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9479303.1"
                     /translation="MPYLLTILILLPFAGALALVLYSFAPPRRDNHYRWIALVATVAT
                     FVVSLLLLRGAGGSGAEFRFEENVNWIGSIGARYHLGADGVSLWLVLLTTLLMPIAVL
                     SSWTAISKRPLAYYAFLLILESAMIGVFVSLDLLLFYLFFEASLVPMFFLIGIWGGER
                     RIYAAIKFFIYTAVGSLLMLVGIIALYFIYDTFDYPTLLQALTASPLTPRAETILFWG
                     FALAFFIKVPLFPLHTWLPDAHTEAPTAGSVILAGILLKMGTYGLLRFNLALFPNASR
                     WWALLIIILAVIGIIYGALVAMVQPDVKRLVAYSSVSHMGFVVLGLFSFTEYGMQGAL
                     YQMLNHGVSTGALFLFVGFIYERRHTRMISDFGGLATPMPWFSTLFVIASLSSIGLPF
                     LNGFVGEFLILLGTWVSTAVTNSWIVTMLAATGVIWAAVYMLWMLQRVVFGEVTNPEN
                     ARLKDLNARELGLLLPLMLLMLFMGVYPRVFLDRSRPSVQAIGDRVNTTPAGGSFATA
                     EVKRVD"
     gene            complement(3043..>4625)
                     /gene="nuoL"
                     /locus_tag="VFO72_08170"
     CDS             complement(3043..>4625)
                     /gene="nuoL"
                     /locus_tag="VFO72_08170"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9479304.1"
                     /translation="DNFVLMFVGWEGVGLCSYLLIGYYLKRKEAGDAAKKAFITNRVG
                     DWGFLLGMMLTFFVTGSVSFFANPAAGEAAQTALSYFRTQPVEPFTLGAIFAGGATSI
                     AILLFIGAIGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVTAGVYMIVRCSEIFVHA
                     PTAMFIVAVIGAATALFAATIGLAQNDIKKVLAYSTISQLGYMFLACGIGAFIAAIFH
                     VITHAFFKALLFLGAGSVIHGMHHEQDMRRMGGLRKYMPITFATMITGWLAISGVPIF
                     AGFFSKDEILWKTWSAALGPWSKVLWAVGAITALLTALYMTRMMVMTFWGEERFKDSE
                     VHPHESSWVMTVPLIVLAILSTIGGFIGVPYAFANRDVNVIEHTLEPAVAKLPTSVDT
                     DHADEHAEVGEERLLAVVSVVIALLGIGAGWVLFQRRPLLQMPRVLENKYYVDEIYDT
                     TVIRPVEVISREGFWKIFDIGVIDGILHSIGDAVTEAGRIARYLQAGFVRAYAAIILV
                     GALILIGLFAYFGFPLAR"
BASE COUNT         1222 a         1336 c         1262 g          805 t
ORIGIN      
        1 gttaagcact ccgatgacga cgagtgtgta atatccctga tcgattgccg cgccaaacac
       61 cataaccttc ccaatgaagc ccgctgttag cggcatgccc aaaagactca gcataaagag
      121 gctcaacgag aaggcaagca ccggcgattc gaaaccgata ccgctgtagt cttcgactga
      181 cgtacggcga tcaccgctgc gggcgatgag ttggaccaca gcaaacgcgc cgatgttcat
      241 caccgcgtaa gtgagcagat agaacatcac ggccgtgatt gccgaattgc gttgagttac
      301 atcagtcgct gcgcctgcgg cgacaacgcc caccagcgca taacccgcgt gtgcgatcga
      361 cgagtacgcc agcattcgtt ttatgttgtt ctggacaatg gcgacgacgt tgccaagtgt
      421 catcgtgagg attgccatca cgaccagcgt agtgacccac acctgatgaa gattgccggc
      481 ggttgcgttc gaggcagaga cgacaaacgg caacccgaag atgaacacgc gcatgaacga
      541 ggcgaaacct gccgctttcg gaccggcggc catgaaggca gtgactggtg tgggcgcgcc
      601 ctcgtaaaca tccggcgtcc agatgtgaaa aggcgcggtg gcgatcttga aaccgaaccc
      661 aacgagcatc atcgccgcgc ccgcgtacag gagcgcggga tattgtgcct ggttgatgcg
      721 gctggcgatc tccgcgatgt tggttgttcc ggctacgatt cggctgaggc tttctcccgg
      781 tcctggttcg gcgatcgagg tcgcgccgta aacgagggcg ataccgtaga gcagaaacgc
      841 ggacgagaag gaaccgagaa tgaaatactt caaagacgat tcatttgaac gaatatcggt
      901 gcgacgaaat ccggcaagca cgtaagtggc aatcgagaga atctcgaggc cgagaaaaat
      961 gatgaccagg tcgttaccgg aagccatcag catcatgccg acagtcgcaa acaaaagcag
     1021 cgagtgaaat tcaccggccg gcagttgttc gtactcgacc caaacaatcg agagcagcaa
     1081 cgtgagcgcg gagacgagca ggaagattag ggtgaagcca aggcgtagtt cgtccagcgc
     1141 gatcatgccg ttgaaagcgt ctgctgaggg cgctccgttt acccaaaggt aaatgcttac
     1201 agcgccggca agtaacaatc cgaccagcga gatgttgccg gtaatccaac gctgcgccgg
     1261 ccgcacaaac gcgtccacga gcatcactac gaccgccgcc gcgcatacaa tcatttccgg
     1321 cgcgatcaac caccagttga tgatcggcat tactgattgc gcaaaaaaga aactcatctt
     1381 caactcatga cagctctttc ctgggaccgc aggcgtctcg cctgcactcc cacaaaaagg
     1441 caacttacgt ttcagggcag gcgagacgcc tgcggtccca ggaaagagca caacctagtc
     1501 aactctttta acttccgcgg tggcgaaaga cccgcccgcc ggagtcgtgt tcacgcgatc
     1561 gccaatcgct tgcacactcg gtctcgatcg gtcgagaaat actcgcgggt aaacacccat
     1621 gaacagcatc agcaacatca gcggcaataa cagtcccagt tcacgagcat tcagatcctt
     1681 tagtcgtgca ttctcaggat tcgtaacttc gccaaacacg acacgctgca acatccaaag
     1741 catgtaaacc gcggcccaga tcacgccagt ggccgcgagc atcgtcacga tccacgaatt
     1801 tgtcaccgcc gttgataccc acgttcccaa caaaatcagg aattcgccga cgaacccatt
     1861 caagaacgga agaccaatcg acgacagcga agcgatcaca aataacgtcg agaaccacgg
     1921 catcggtgtc gccagcccgc caaagtcgct aatcatgcgc gtgtgacgcc gttcgtagat
     1981 gaagccgaca aaaaggaaga gtgcgccggt cgagacgccg tgattaagca tctggtagag
     2041 cgcaccctgc atgccgtatt cggtgaaaga aaacagtcca agcacgacga agcccatgtg
     2101 actgactgac gagtaggcga cgagccgttt aacgtcaggc tggaccatcg cgacgagcgc
     2161 gccgtagatg atcccaatga ccgcgagaat gattatcagc aacgcccacc agcgcgacgc
     2221 attgggaaag agcgccagat tgaaacgcaa cagtccgtac gttcccatct tcagcaagat
     2281 gccggcaaga atcaccgaac cggccgtggg cgcttcagtg tgtgcgtccg gcagccaggt
     2341 gtggagtgga aacagcggaa ccttgataaa gaaagcgagt gcgaagcccc agaacagaat
     2401 ggtttcggcg cgtggcgtta gcggactcgc agttagcgcc tggagcagcg tcggatagtc
     2461 aaacgtgtcg tagatgaaat agagcgcgat gatgccgacg agcatcaaca ggctcccgac
     2521 tgccgtgtag atgaaaaact tgatcgcggc gtagatgcgg cgctcgccgc cccagattcc
     2581 gatgaggaag aacatcggca ccagcgacgc ctcgaagaac aagtagaaca gcagcagatc
     2641 gagcgagaca aacacgccga tcattgccga ttcgaggatc aacagaaacg cgtaatatgc
     2701 cagcggccgt ttgctgatgg ccgtccagct cgatagcacc gcgatcggca ttaacaacgt
     2761 cgtcaacaac accagccaca gactgacgcc atctgcgccg aggtgatagc gggcgccgat
     2821 cgagccgatc cagttgacgt tttcctcaaa cctgaactcc gcgccactcc cgccggcgcc
     2881 gcgcaacagc aacagcgaca cgacaaacgt agccactgtt gcgacaagcg cgatccagcg
     2941 atagtggttg tcgcgtcgcg gaggcgcaaa gctgtagagc acgagcgcaa gcgcacccgc
     3001 gaacggcaac aatatcaaga tggttaacaa atacggcata gactaccgtg ctaaaggaaa
     3061 tccgaaatac gcaaacaacc cgatcaaaat aagtgcgcca actagaatga tcgcggcgta
     3121 ggcgcgaaca aaaccggctt gcagataccg agcgattcgt ccggcttcag ttactgcgtc
     3181 gccaatcgaa tggagaattc cgtcaataac accgatgtcg aatatcttcc agaacccttc
     3241 acgcgaaatc acttcaacgg gacggatcac cgtggtgtcg taaatctcgt cgacgtaata
     3301 cttgttctcc agtacgcggg gcatctgtag cagcggacgc cgctggaaga ggacccaacc
     3361 cgcgccgatt cccagaagcg cgatgacgac cgacacaacc gccagcaatc gttcctcgcc
     3421 aacctcggcg tgttcatccg cgtgatctgt gtccactgaa gttggaagtt tggcgaccgc
     3481 cggttccagc gtgtgctcaa tcacgttcac atcacgattt gcaaacgcat aaggcacacc
     3541 gataaaacca ccgattgtag acaggatcgc cagcacgatc agcgggactg tcatgaccca
     3601 ggacgattca tgcggatgaa cctcagaatc tttgaagcgt tcttcgcccc agaacgtcat
     3661 caccatcatg cgcgtcatgt acaaagcggt tagcaacgcc gtgatcgcgc cgacagccca
     3721 aagaactttg ctccaaggac ctaaagccgc gctccaagtc ttccagagaa tctcgtcctt
     3781 tgaaaagaag ccggcgaaga tcggaacgcc gctgatcgca agccagcccg tgatcatcgt
     3841 tgcgaaagtg atcggcatgt attttcgcaa accgcccatg cgccgcatat cctgctcgtg
     3901 atgcatcccg tgaatcactg agccggcacc gaggaacagc aacgctttga agaacgcgtg
     3961 cgtgataacg tgaaagatcg cggcgataaa agcaccgata ccgcaggcca gaaacatgta
     4021 accgagctgc gaaatcgtgg agtaagcaag taccttttta atgtcgttct gcgctaagcc
     4081 gatcgtcgct gcaaacaaag ccgtggccgc gccaatgacc gcaacgataa acatcgctgt
     4141 cggcgcgtgc acaaagatct cagagcagcg cacgatcatg tagacgcccg cggtcaccat
     4201 tgtcgcggcg tgaatcaacg ccgagacggg cgtcggacca gccatcgcat ccggcagcca
     4261 gacgaagagc gggatttgcg cgctcttacc gatggcgccg ataaagagca gtattgcgat
     4321 ggacgtcgca ccgccggcga agatggcgcc cagtgtgaac ggttcaactg gttgagttct
     4381 gaaataagaa agcgcggtct gcgcggcctc gcccgccgcg gggttggcaa aaaaactgac
     4441 acttccggtg acgaagaagg tcagcatcat gccgagtaga aaaccccagt cgccgacgcg
     4501 gttcgtgatg aacgctttct tcgccgcgtc tccggcttct ttgcgtttca agtagtagcc
     4561 gatcagaagg tacgagcaga gaccgacgcc ttcccagccg acaaacatca acacgaagtt
     4621 gtccg
//
LOCUS       DASTEY010000466         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_339810, whole genome shotgun sequence.
ACCESSION   DASTEY010000466 DASTEY010000000
VERSION     DASTEY010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339810"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFO72_08175"
     CDS             <1..355
                     /locus_tag="VFO72_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479305.1"
                     /translation="TQAPAHTPSPTHTRQPPHAQTPAERRQPIPTVRNNVEVLSDRFM
                     DRTIPQAVTLLLREEGGPLHVNEIYNRLLEGGFRFTGQNPTISVAVSLNRNSRFRKVA
                     PGTFDLVMRDASKAS"
     gene            368..1336
                     /locus_tag="VFO72_08180"
     CDS             368..1336
                     /locus_tag="VFO72_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET9479306.1"
                     /translation="MRAIILSAGYGTRLWPLTEDRTKPAIPILGKPLVGYVAEYIASY
                     GFDDIVVNLHHRPQSVRAALGDGSKFGVKLRYVEEPVILGTSGALDNTREFFEHETFL
                     AINGKIITDIDLNAALKTHRNSQALATLVLLPNTKRERFSVVKVDRGRITGFGRMPDS
                     QKDGPPPLMFTGMHIFEPRIFDYIPRGVFSDSVIDVYPKAIANGETIAAHVAAGTWRE
                     LSTLKRYVDISVEMLNERGQRYVAGSNCAIATSAEIDESILWDNVSVGANTRVSRSVL
                     ADDVQIPDATVIADCVVVPRALVEGKTRPEKALSGHFQGGNFIVPL"
     gene            1371..2411
                     /locus_tag="VFO72_08185"
     CDS             1371..2411
                     /locus_tag="VFO72_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9479307.1"
                     /translation="MSSAKQVISKAFESSAADRLARFITQQGITSREVVPLTPDASTR
                     KYYRVGWKKGTAVAAVYPEPFDPEFHPYLDVTRLFLECDLPVPEIYAVDGAAGIIVQE
                     DLGDQQLFQVYESESEDRCDEYKEQAIALIAKLQKATQKAYDMHSIASRLAFDEAKLS
                     WELDFFFEHYFGSLRGETLRHAETAELKAELNDITAELAAVPRVLCHRDYHAANLMID
                     KKKRMRIVDHQDARMGPASYDLVSFLLDRQPCLPSLAELRAYRLFFLEERRQQGLATI
                     DPDDFAQEFRLMTIQRGLKATGTFSCQTAVFGRGAAYERFIQPTLQIVLQAAEWLDRF
                     PTLRRMIKERIN"
     gene            2414..3730
                     /gene="asnS"
                     /locus_tag="VFO72_08190"
     CDS             2414..3730
                     /gene="asnS"
                     /locus_tag="VFO72_08190"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET9479308.1"
                     /translation="MSTHTYINQLAKHVGSEVTLRGWLYNMRSSGKILFPQLRDGTGL
                     VQCVVLKNAVDPELWEQLKGMGQESALAIRGTVRADSRAPGGYEIDVVGAEVLQQVHD
                     YPITPKEHGTEFLMDHRHLWLRSRRQHAVLKVRHTVVQAVRNFLDNDGFTLADSPIFT
                     PAACEGTTTLFEVDYFEGEKAYLTQSGQLYNEATAAAFGKSYCFGPTFRAEKSKTRRH
                     LTEFWMVEPEMAYATLDDVMNLSERMLSYIATRVLEDRSEELKVLERDTSKLEGIVPP
                     FPRLHYDDAAKMLVEGFEKGALESKFEWGGDFGAPDETYISSKFDKPVMVHHYPAAVK
                     AFYMARDPERPELALGVDVLAPEGYGEVIGGGERATSLKFLEEQIALHELPQEAFEWY
                     LDLRRYGSVPHGGFGMGIERCTAWMCGIEHIRETIPFPRMLYRIRP"
     gene            3998..4741
                     /locus_tag="VFO72_08195"
     CDS             3998..4741
                     /locus_tag="VFO72_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET9479309.1"
                     /translation="MQIDQDSRGQGRGYQYQVLHQPSFSLAVLQLQPEQSIQAESGAM
                     VSMSANVELQSQMKGGIFGAIKRAAGGESAFISTFTARGGPGEVTLAPGAPGDIAAVE
                     LSGQPFFVQSSSYLAGDASLTVDTKWGGAKSFFSGEGLFVLLVQGQGLLLVSSFGAIH
                     RKQLRAGERYVVDTGHLVAWEGSMQYSLRKAASGIFRSMMSGEGIVAEFSGPGELLIQ
                     TRNLAALAGLLRPFFPSQGGGGGGFNLDF"
     gene            4855..>5241
                     /locus_tag="VFO72_08200"
     CDS             4855..>5241
                     /locus_tag="VFO72_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET9479310.1"
                     /translation="MSAQSQKAVTILGVPLGYGASMAGVDIGPAALRVARLTQRIARL
                     GYSVRDLGDLRLERPQAFPEDSDKLKYVSEISNACEQLAGEVEAILEANEFPIILGGD
                     HSIAVGSFAGAANYFRRREETLGLIWF"
BASE COUNT         1167 a         1447 c         1526 g         1101 t
ORIGIN      
        1 cacgcaagca ccggcgcata cgccatcacc aacgcataca cggcaaccgc cgcacgcgca
       61 aacaccggcc gaacgccggc aaccgatccc aaccgttcgt aacaacgtcg aagtgctcag
      121 cgatcgcttc atggaccgca ccattccgca ggcagttacg ctgttgctgc gtgaagaagg
      181 tggaccgctg cacgtcaacg aaatctataa ccgtctcctc gagggcggtt tccgtttcac
      241 tggacagaac cctaccatca gcgttgcagt ctcgctcaat cgcaattcgc gctttcgcaa
      301 agtcgcgccc ggcacctttg atctcgtgat gcgcgacgcc tcaaaggctt cctgaagggg
      361 ccaggcgatg cgcgcgataa ttctttccgc cggttacggc acacgtctct ggccgctcac
      421 tgaagatcgc accaaacccg caattccaat tctcggcaaa ccgctcgtcg gttatgttgc
      481 cgagtacatc gcgagctacg gtttcgatga catcgtcgtg aacctgcatc accggcccca
      541 gtcggttcgt gcggcgcttg gcgacggcag caagtttggc gtcaaactgc gctacgttga
      601 agaacccgtg atcctcggca ccagcggcgc gctcgacaac acccgtgaat ttttcgaaca
      661 cgaaacgttt ctcgctatca acggcaagat catcacggat atcgacctca acgccgcgct
      721 caaaacgcac cgcaactccc aggctctcgc gacgctcgtc ctgctgccaa atacaaaacg
      781 cgaacgtttt agtgtggtga aagtcgatcg cgggcgcatc acgggtttcg gccgaatgcc
      841 tgacagccag aaagacggtc cgccgccact gatgtttaca ggcatgcaca tctttgaacc
      901 gcggatcttc gattacatcc cacgcggcgt cttctcggac tccgtgatcg acgtttaccc
      961 gaaagccatc gccaacggcg aaacgatcgc tgcgcatgtc gccgccggaa catggcgtga
     1021 actctcaacg ctcaaacgct acgtcgatat cagcgtcgag atgctgaacg aaaggggaca
     1081 gagatacgtc gccggcagca actgcgccat agccacatcc gccgagatcg acgaatcaat
     1141 tctgtgggac aacgtcagcg tcggcgcgaa cacacgtgtc agcagatcag tgttggcaga
     1201 cgacgtacaa attccggatg ccaccgtcat tgctgactgt gtggtcgtcc cgcgcgcgct
     1261 ggtcgaagga aaaacgcggc ctgagaaagc tctcagcggc cattttcaag gcggaaactt
     1321 tatcgtgccg ttgtgataca atcccggcca catcagggtt gtccgactac atgagctctg
     1381 caaaacaggt gatctccaaa gcatttgagt cttccgcagc agaccgactg gcgagattca
     1441 tcacgcagca gggaataacc agccgcgaag tagtcccact gactcccgac gcgtcaaccc
     1501 gcaagtatta ccgcgtcggc tggaaaaagg gcaccgcggt tgccgccgtc tatcccgaac
     1561 cgttcgatcc cgaatttcat ccctatctcg acgtaactcg actgtttctg gagtgtgacc
     1621 ttccagtccc tgaaatctac gcggtcgatg gcgcagcggg cattatcgtt caggaagatc
     1681 tcggcgatca gcagttgttc caggtctacg agagcgagtc cgaagacagg tgtgacgagt
     1741 acaaggaaca ggcaatcgcg ctgatcgcta aactccagaa ggcaacccag aaagcctacg
     1801 acatgcattc aatcgccagc cggctggcgt ttgacgaagc aaaactttcg tgggagttgg
     1861 actttttttt cgaacattat ttcggcagct tgcgaggcga gacgttgcgg catgcggaaa
     1921 cagcagagtt gaaggcagaa ctgaacgaca tcacagcgga gctggcggcc gttccgcgcg
     1981 tgctttgtca tcgcgactac catgcggcga acttgatgat cgacaagaag aagcgaatgc
     2041 gaatcgtgga tcatcaggac gcgcgcatgg gccctgcaag ttacgatctg gtctcgttcc
     2101 tgcttgatag acaaccgtgt cttccgtcgc tggcagagtt gcgcgcgtac cgtctgttct
     2161 ttctcgagga acgccggcaa cagggactcg ccacaatcga tcccgacgac ttcgcgcagg
     2221 aatttcgcct gatgacgatt caacgcggct tgaaagccac gggcaccttc tcctgccaaa
     2281 cagccgtgtt cggccgcggc gcggcttacg aacgtttcat tcaaccgact ttgcagatcg
     2341 tactccaggc cgccgaatgg ctcgacaggt ttcccacgtt acgccgcatg atcaaggaac
     2401 gaatcaacta actttgagca cccacaccta tattaaccaa ctcgcaaaac acgtcggcag
     2461 tgaagtaacc ctaagaggct ggctctacaa catgcgctcc agcgggaaga ttctctttcc
     2521 gcaactgcgc gacgggacgg gcctggtcca atgcgtggtg ttaaagaatg ccgtcgatcc
     2581 cgaattgtgg gaacagttga aaggcatggg ccaggagtcg gcgttggcga tcaggggaac
     2641 tgtgcgtgcc gacagtcgtg cgccgggcgg ctacgagatt gacgtcgttg gcgccgaagt
     2701 tttgcagcag gttcatgatt atccgatcac gccaaaagag cacggaactg agtttctcat
     2761 ggaccaccgg cacctttggt tgcgctcgcg tcggcagcac gcagttttga aagtgcgtca
     2821 caccgtggtt caggccgtaa ggaactttct cgataacgac gggttcacgt tagccgactc
     2881 acccatcttc acgcccgccg cttgtgaagg caccacaacg ctctttgaag tcgactattt
     2941 tgaaggtgag aaggcttacc tgacgcagtc aggccaactc tataacgaag cgaccgcggc
     3001 ggcgtttgga aaaagttact gttttggtcc aacgttccgg gccgagaagt cgaagacccg
     3061 gcggcatttg acggagttct ggatggtcga gcccgagatg gcctacgcga cgctcgacga
     3121 cgtgatgaat ctttccgaac gaatgctttc gtacatcgcc acgcgcgtgc ttgaagatcg
     3181 cagcgaagag ttgaaggtgc tcgaacgcga cacgagtaaa ctcgaaggga tcgtgccacc
     3241 gtttccgcga ctccattacg atgatgcagc gaagatgctc gtcgagggat ttgagaaagg
     3301 cgcgttggag tcgaagtttg agtggggcgg agactttggt gcaccggatg aaacttacat
     3361 ctcgagcaag ttcgacaaac cggtgatggt tcatcactat ccggccgcgg ttaaagcgtt
     3421 ctacatggcg cgcgatccgg agcggccgga gttggcgttg ggagtcgacg tactcgcgcc
     3481 ggaaggttat ggtgaagtca ttggtggcgg tgaacgtgcg acgtcactga agtttctcga
     3541 ggagcagatc gcgttgcacg agttaccgca ggaggcgttt gagtggtatc tcgatctgcg
     3601 gcgttacggc agcgtgccgc acggcgggtt cggaatgggg attgagcgct gcacggcgtg
     3661 gatgtgcggg atagagcaca ttcgcgagac gataccgttt ccgaggatgc tctatcgcat
     3721 tcggccgtaa ttgaatggtg tcctactctg aacgccagag gcgttcgcta acttcagccc
     3781 ggggttggaa caaccccggg tcgacgttag aacccagtcc taacgctgaa agcgttggca
     3841 aactcgcggt tactcgcgaa cgctttcagc gtaagacggt aaacacatcc aatccgtacc
     3901 cagggttgtc gcgagcgcca acccctgggc tggaattcga taacgccttc ggcgttccga
     3961 ttggggcact acccgtaatt gtgaaggagt cgggattatg cagattgacc aagattcgcg
     4021 cgggcaggga agaggttacc agtatcaggt gttgcatcaa ccatcgttct cgcttgcggt
     4081 gttacagctt cagccggaac aatcgattca ggcggagtcc ggcgcgatgg tatcgatgtc
     4141 agctaatgta gaacttcagt cgcagatgaa aggcggcatc tttggcgcga tcaaacgcgc
     4201 tgcgggtggc gagtcggcgt tcatctcgac atttacagca cgcggtggtc caggtgaagt
     4261 gacgttggcg ccgggtgcgc cgggcgatat cgcggcggtc gagttgagtg gccagccgtt
     4321 cttcgtgcag tcgagttcgt atcttgcggg tgatgcgagt ctcacagtcg atacaaaatg
     4381 gggtggcgct aagagtttct tctcaggaga aggattgttt gttctgctcg tgcagggtca
     4441 gggattgttg ctcgtttcct cgttcggcgc gatacaccgg aagcaactgc gagcgggtga
     4501 acgttacgtt gtagacactg gccatctggt cgcgtgggaa ggctcaatgc agtactcgct
     4561 gcgaaaagcg gcgtcgggca tcttccgcag catgatgagc ggcgaaggca ttgtggccga
     4621 gtttagcggg cctggcgaac tgctgattca aacacgaaac ctcgcggcgc tggctgggct
     4681 gctcaggccg ttcttcccaa gccagggcgg cggaggcggc ggattcaatc tcgacttcta
     4741 agattcgagc ttggtacttt gagctttgtt ctttgtatct taatgtgagc cttccaccag
     4801 ggacggcgaa caaagtgaca aaagaacaaa gtactaagct ctgaatgcga gcgaatgtct
     4861 gcacaatcgc aaaaggcggt cacgattctc ggcgtgcctc tcggctatgg cgcaagcatg
     4921 gcaggagtgg acatcggtcc tgccgcattg cgtgtggcgc gtttgactca acgcatcgcc
     4981 cgtctaggtt actccgtccg cgatcttggc gacctgcgtc tcgaacgacc tcaagcgttt
     5041 cctgaagata gtgacaaact gaagtacgtg agcgagatca gcaacgcctg cgagcagctt
     5101 gcgggcgaag ttgaagcaat acttgaagcg aatgagtttc cgatcattct gggcggcgat
     5161 cattcgatcg ccgtcggatc gtttgcgggc gcggcgaatt attttcgcag acgggaagag
     5221 acgctgggcc tcatctggtt t
//
LOCUS       DASTEY010000467         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1015443, whole genome shotgun sequence.
ACCESSION   DASTEY010000467 DASTEY010000000
VERSION     DASTEY010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015443"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFO72_08205"
     CDS             complement(<1..388)
                     /locus_tag="VFO72_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9479311.1"
                     /translation="MSSVLIRGMIATMDDLRKFARYFSKYKLQLALGIVCIFASVVAG
                     LFIPLSVGQAIDENWNQVTWSALTISAGKVLVASLISGIFLFLQRRILIGTSRHIEYD
                     LRQDFYGHLVNQPLSFFHEHRIGDLMA"
     gene            complement(431..853)
                     /locus_tag="VFO72_08210"
     CDS             complement(431..853)
                     /locus_tag="VFO72_08210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9479312.1"
                     /translation="MITPDLNEEQRARVEAMREAVPFLELLGVEIESVGPGTATLLLP
                     IREKIKRNDGIVHGGAIASVIDSAFALAITPLLGENERTTTVDLTIHYLRPLSAGVAK
                     TTARVVRLGRRVITVSAELFDENGKLAATALSTYLRIS"
     gene            complement(850..1260)
                     /locus_tag="VFO72_08215"
     CDS             complement(850..1260)
                     /locus_tag="VFO72_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HET9479313.1"
                     /translation="MSSDAVQRAPYAVSPPRYSVSHQMVTTTFELPNYRVVQNLGVVR
                     GIVVRSRNIFATIGASLQTVVGGNITVWTSLCEQTRADAFEIMIQHATEIGANAVIGA
                     RYDTTELSTGVTEVLAYGTAVIVEPLNPGETFRS"
     gene            complement(1257..1637)
                     /locus_tag="VFO72_08220"
     CDS             complement(1257..1637)
                     /locus_tag="VFO72_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET9479314.1"
                     /translation="MTRQGNPNYRERVYKIVRRIPRGRVMTYGQIAELLGEGYTARTV
                     GFAMHASPDGMPWHRVLNAQGACSTGRVVLPHDKQQRLLEQEGVVFNKNNRCDLQTYL
                     WIPKTKAAPREASLYKSSKRGGKL"
     gene            complement(1634..2311)
                     /locus_tag="VFO72_08225"
     CDS             complement(1634..2311)
                     /locus_tag="VFO72_08225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9479315.1"
                     /translation="MRLSVYYYSPMPIYDLSVPLSAELATYPGDPGIRIQDWSKLANG
                     DGANVSSLSFGAHTGTHVDAPAHFIEGAKRLESLDLEILIGEAQVVQVPEELRVIDEE
                     FVAANCAVGTNRVLFKTRNSAFWTTDFTDFRTDFTYLDLRAAERLVDQGVKLVGIDYL
                     SIEKFRSKDHEVHRALLSHEVIIVEGLNLAGVPAGKYELICLPLRLRTNLGDGAPARA
                     VLRTLNR"
BASE COUNT          550 a          677 c          633 g          519 t
ORIGIN      
        1 gcgccattaa gtcgccaatg cgatgttcgt gaaagaacga gagaggctgg ttgaccagat
       61 gtccgtaaaa atcctggcgc aggtcgtact cgatatgacg cgacgtgcca atgagaattc
      121 gccgttgcag aaaaagaaag atgccgctga ttaggctggc cacgagaacc ttcccggcgg
      181 aaatggtcag cgccgaccat gtaacttgat tccagttctc gtcgatggct tgtccgacac
      241 tgagcggaat aaacagaccc gcaactacac tggcgaagat gcagacgata ccgagcgcta
      301 gttggagttt gtatttacta aagtagcgcg caaacttcct gagatcatcc atcgtcgcta
      361 tcatgccacg gattaacacg gatgacacga actgtgccaa tttgtctgtg ttatccgtgt
      421 ttgtctgtgg ctaactaatg cggaggtagg tgctgagggc agtagcggca agctttccgt
      481 tttcgtcaaa caactcggct gaaactgtaa tcacacggcg tcccagacgc acgactcgcg
      541 cagtcgtttt cgcaacgccg gcactcagcg gacgcaggta atgaattgtg agatcgaccg
      601 tcgtcgtgcg ctcgttttct ccaagcagag gagtgatcgc caacgcaaac gcggaatcga
      661 tcacggaagc gatcgcgcca ccgtgaacga ttccatcgtt gcgcttgatc ttttcacgaa
      721 ttggaagcaa cagcgttgct gttccgggtc cgacagactc aatttcgact ccgaggagct
      781 cgaggaaggg aaccgcttcg cgcattgctt caactcgcgc gcgttgctct tcgttcagat
      841 ccggcgttat catgaacgga aagtctctcc ggggttcaag ggctcaacaa tgaccgccgt
      901 gccataggca agcacttccg tgacgcccgt tgacagttcc gtcgtatcgt aacgcgcacc
      961 gatcaccgcg ttcgcgccga tctcggtggc atgttggatc atgatttcga aagcgtcggc
     1021 gcgcgtctgt tcacaaaggc tggtccaaac ggtgatgttt ccaccgacga cagtctgaag
     1081 cgaagcgccg atggtcgcga agatgtttcg cgaccgcacg acaataccgc gaacgacgcc
     1141 gaggttttgc acgacgcgat aattcggcaa ctcgaatgtg gtcgtcacca tttggtgaga
     1201 aacggaataa cgcggtgggc taacggcata gggagcgcgt tgcacggcat cggaactcat
     1261 aattttcctc ctcttttgga agacttatac agactcgctt cgcgtggcgc ggctttcgtt
     1321 ttcggaatcc acaggtacgt ttgcagatcg cagcgattgt tcttattgaa gaccacgccc
     1381 tcctgttcca gcagacgttg ctgtttgtcg tgcggcaaca cgacgcgtcc ggtcgagcac
     1441 gcgccctgcg cgttgagcac gcgatgccac ggcataccgt cgggcgaagc gtgcatcgcg
     1501 aagccgacgg tgcgagcggt ataaccttcg cccaacagct cggcgatctg cccgtaggtc
     1561 atcacgcggc cacgcggaat gcgacgaacg atcttataaa cgcgttcgcg gtagtttggg
     1621 tttccttgtc tggtcatcgg ttcagagttc tcaacacggc gcgggccggc gcgccgtcgc
     1681 cgagattagt gcgcaaacgc aacggcaaac agatcaactc gtactttccc gccggcacgc
     1741 ccgcaagatt caacccctcg acaataatca cttcgtgcga caacaacgca cgatgtactt
     1801 catggtcctt cgagcgaaac ttctcgatcg acaaataatc aatccccacc aacttcaccc
     1861 cttgatctac aagtctctcc gccgcgcgca aatccaaata cgtaaaatcc gttcggaaat
     1921 ccgtaaaatc cgtggtccag aaggccgagt tgcgggtctt gaaaagcacg cggtttgttc
     1981 ccaccgcaca gttcgcggcc acaaactctt cgtcgataac tcgaagctct tccggaacct
     2041 ggaccacctg agcctcgcca atcaggatct cgagatccag cgactccaac ctttttgcgc
     2101 cttcgatgaa atgtgcagga gcgtcgacgt gagttcccgt gtgcgcgccg aaactaagcg
     2161 aagaaacgtt agcgccgtcc ccgttggcaa gtttggacca gtcctggata cgaattccgg
     2221 gatctccggg ataggtcgcc aactcggcgc ttaaaggaac actcagatcg taaattggca
     2281 tcggcgaata gtaatatact gaaagccgca tgactacacc gtttcgactc gccgcatcca
     2341 gttacctgaa ctccgcaccg ctgatctgga gctttcttt
//
LOCUS       DASTEY010000468         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_603181, whole genome shotgun sequence.
ACCESSION   DASTEY010000468 DASTEY010000000
VERSION     DASTEY010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603181"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1104)
                     /locus_tag="VFO72_08230"
     CDS             complement(1..1104)
                     /locus_tag="VFO72_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479316.1"
                     /translation="MARFKRTVAVLLLTIAATYTTAQQQPKNQDQPVRVFTEEVRLPV
                     VALDAYGHYDPSLELEDILVLEDGVAQQLRSLRHIPANVLFVLDTGGELTGLGGRSKS
                     TSLTRQVASELVKRLQTETSIAVMQSGNTAELLQPWTTDKAAVLKTLKNKLISTKRSR
                     ISEAIVKATQQLRDRPEGSRHIVMITDGVDSAGAKIGPAEAMSKLIAARATVHIISYT
                     EFVRQKGDEKDSGIVTGQRPTTSDPITATDPTLPPGTTRSPSFGIGIRFDPAMRRRRK
                     AYEAEVKKSEQALKNVAEETGGKWLLPVNSEEMLAQATDVARAISAEYVVTYRPKRPL
                     AEARPGEYRRIEVASRRPGLSLQARRGYVVPTP"
     gene            complement(1138..2145)
                     /gene="pta"
                     /locus_tag="VFO72_08235"
     CDS             complement(1138..2145)
                     /gene="pta"
                     /locus_tag="VFO72_08235"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET9479317.1"
                     /translation="MDILERIRQRAASRPQHIVLPEGNDPRTVVAAAHCVNQRLARIT
                     LLGDEEVIRTTALENNLDLGGVAIVDHRRAADFEKMASLIHDLRRAKGLMADEARTLV
                     SDPLYYGNLMVRLGQADGSVAGATNTTSHTVRAALHCIGVRSGFKLVSSFFLMSVPRS
                     EFGHDGALIYADCGVVIEPSAAELAEIALAAADSCKALLGTEPRVAMLSFSTKGSARH
                     KLIDKVVEATRTVKARAPELQVDGELQADAALVASVGQAKAPGSPVAGRANVLIFPDL
                     QAGNIAYKLTERLAGATAIGPILQGLDKPANDLSRGCTARDIVDAVAITAVQAQARKT
                     N"
     gene            2170..>2480
                     /locus_tag="VFO72_08240"
     CDS             2170..>2480
                     /locus_tag="VFO72_08240"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET9479318.1"
                     /translation="MSEVKLRSRMKNLLLFIPNLMLLCARLMIDPRVPATERVLVAGA
                     ILYALIPLDFIPDMLPFVGQVDDAYLIAISLLRLMTVTDPRVVRQHWRGGGDYGARIV
                     A"
BASE COUNT          507 a          722 c          690 g          561 t
ORIGIN      
        1 tcagggtgtg gggacgacgt agccacggcg cgcctgaagg cttagaccag gtcttcgcga
       61 agcgacttca atgcggcgat attcgcccgg cctggcttcc gcaagcgggc gcttcggacg
      121 ataagtcacg acatactcag cgctgatcgc gcgagcgacg tcagtagcct gcgcgagcat
      181 ctcctcgcta ttaacaggca acagccattt cccgccggtc tcttcagcga cattcttcaa
      241 cgcctgctca ctcttcttta cctcagcttc ataagctttc cgtcgccgtc gcatcgcggg
      301 atcgaatctg atcccgatgc cgaaagaagg tgaacgagtt gtgcccggcg gaagggtcgg
      361 atcggtcgcg gtgatcgggt cggaggttgt gggccgttgt ccggtcacga ttcccgaatc
      421 cttctcgtcg cccttctgtc gcacaaactc cgtgtagcta atgatatgca ccgtagcacg
      481 ggcggcgatc aacttactca ttgcttccgc agggccgatt ttggcgcccg ccgaatcaac
      541 gccgtctgta atcatcacga tatgccggct tccttcagga cgatctctca actgttgcgt
      601 cgccttaaca atggcttccg agattcgcga tcgttttgtc gaaatcagtt tgttcttcag
      661 ggttttcaaa acagccgctt tgtcagtggt ccagggctgc aacagctccg ccgtatttcc
      721 cgattgcatc acggcgattg atgtttcggt ttgaagtctt ttcaccagtt cagaggcgac
      781 ctgacgggtc aatgacgtac tcttcgaacg cccgccaagc ccggtcaact caccacccgt
      841 gtcgagtaca aacaagacat tggccggaat gtgtcgcaag ctgcggagtt gctgcgcgac
      901 gccgtcttcg agcacaagaa tgtcctcgag ctcgagactc ggatcgtaat ggccgtaggc
      961 gtcgagcgcg accactggta ggcgaacctc ttcagtaaaa acccgaacag gttgatcttg
     1021 atttttgggt tgttgttggg cggtggtgta ggttgcagcg atagttaaca agagaaccgc
     1081 aactgtacgt ttaaatcgag ccacggattt acacagtcag acagatgcgc ggcgccctca
     1141 gttggttttc cgcgcctgtg cctgcacggc tgtaatcgcg accgcatcga cgatgtcgcg
     1201 tgccgtgcag ccgcgcgaaa ggtcgttagc aggtttatcc aggccttgca ggatcgggcc
     1261 gatggcggtc gcaccggcaa gtctttcggt cagcttgtac gcaatgttgc ctgcctgaag
     1321 atcggggaag atgagaacgt ttgcacggcc ggcaaccggc gagccgggag cttttgcctg
     1381 tcccaccgaa gccacgagag ccgcatccgc ctggagttca ccatcaacct gcaactcggg
     1441 cgctcgtgcc tttaccgtgc gcgtggcttc cacgacctta tcgatcaact tatgtcgcgc
     1501 actgcctttg gttgaaaacg acaacatcgc cactcgcggt tcggtgccca acaacgcttt
     1561 gcacgaatcc gccgccgcca gagcaatctc tgccaactcc gctgcgctcg gttcgatcac
     1621 gacgccgcag tcggcataaa tcaacgcgcc atcatgtcca aactcacttc gcggcaccga
     1681 catcaaaaag aaactcgaga ccagtttaaa tcccgagcgc acgccgatac agtgcaatgc
     1741 tgcccgaacc gtgtgcgaag tggtattcgt cgcaccggcg accgagccgt cagcttgccc
     1801 cagacgcacc atcaggtttc cgtagtacaa cggatctgaa acaagtgttc gtgcttcatc
     1861 ggccatcaag cctttggctc ggcgcaagtc gtgaattaac gaggccatct tttcgaaatc
     1921 cgcagcacgg cggtgatcca cgatcgcaac gccaccgagg tcgagattat tttcaagcgc
     1981 agttgtgcgg atcacttctt catcacccaa caacgtaatg cgtgcgaggc gttgattgac
     2041 gcaatgagcg gcggcgacta cggtgcgagg atcgttgcct tcgggaagta cgatgtgttg
     2101 gggtctggac gcggcgcgtt ggcgaattcg ttcgagaatg tccattgcgt aatctgtata
     2161 atggctggca tgtcagaagt aaagctgcga agccgcatga aaaacctctt gctgttcatc
     2221 ccgaatctga tgttgctctg cgcgcgattg atgatcgacc cgcgcgtgcc ggcaacagaa
     2281 agagttttgg ttgcgggcgc aattctctac gccttaatcc cgctcgattt tattcccgac
     2341 atgctgccgt tcgtgggcca ggttgacgac gcttacctca tcgcgatctc acttctgcga
     2401 ctgatgaccg taacggatcc acgcgtagtg cgacagcact ggcgcggcgg cggcgactac
     2461 ggtgcgagga tcgttgcctt
//
LOCUS       DASTEY010000469         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_982305, whole genome shotgun sequence.
ACCESSION   DASTEY010000469 DASTEY010000000
VERSION     DASTEY010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982305"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFO72_08245"
     CDS             complement(<1..617)
                     /locus_tag="VFO72_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479319.1"
                     /translation="METLIQDIKFGLRMLLKSPSLSIVATIALALGIGANTAIFSVVN
                     AVLLRPLPFPNSESLVALFETDRQRGSLRGSHSYPNFFDLRAQNTSFERVAAYYSSDF
                     IMTGRGEPARLQGTVATADLIPLLGVAPKLGRTFLPDEDQPSATGRVVILSEQLFRTR
                     FNSDPSLLNQTLTLDGVSFTVVGVMPESFEFPIQNEPVELWTTIAG"
     gene            complement(715..>3055)
                     /locus_tag="VFO72_08250"
     CDS             complement(715..>3055)
                     /locus_tag="VFO72_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479320.1"
                     /translation="LQSLAVTNPEQLAVLSSYSKGDPQIDSPFSYPMYQSLRDRNDVF
                     AGVIARGGAQMNVSYGDQTERVRGELVSGNFFEVLGVRPWAGRLFTQEDDRMPGAHPV
                     AVLSYAYWESRFGKDPNLIGKTILVNAHPLTVIGVTPPGFYGVNLQDHPDVRVPMMMT
                     PVFNPLPPTRLQSWRHHWLSLMARRKTAVSSEQAEASLNVLYRQIREAEAQELAASLS
                     ASDREKYVAERIAVAPGDQGFRHLQTELKTSLWLLFGATCAVLLILCANLANLMMARA
                     TGRAQETAVRLALGASRLRLLRQWLTEGIVLAFIGGAVGMVIALWIKSGLMAFTPSDF
                     RVNLGGPLDWRLYAFIFGVAVLLGLAFSLAPAIQAARQVFAPGLRLESRSVTTAGKLV
                     SLRSALILVQVGLSLPLLFTAGLLIKTLQNLRAVDTGFGKENVLLASINPALNGYSPE
                     RTANFYNELLARARTLPGVKYASLATDSPISGGWDQLRVVVEGYTPREGERMFGDFTG
                     VSADYFKTLEIPLVAGRDFNEEDRLGSPRVVIINEKMANHFFGTANPLGKRIGLGKIP
                     DMTIVGVVKDAQYINLRQDRRRHFYLPTAQEKVLTSLTLHVKTETEPATVSESLRTQL
                     KALDPQLPLYDIKTLATEIDESLVQERLVTWLSSAFGLLATLLTAVGLYGVLTFSVAR
                     RTREIGIRVAFGAQRRDVFKLIMIRGVVLVAVGVLVGVSASIAFSRLLESLLFGVAPG
                     NVTMLLLVSAGLIVVALLACYLPARRATQVDPLVALRYE"
BASE COUNT          719 a          905 c          835 g          596 t
ORIGIN      
        1 ccggcgatgg tggtccagag ttcgactggt tcgttttgaa tcggaaattc aaacgactcc
       61 ggcatcacgc caacgacggt gaagctgacg ccgtcgagtg tcagagtctg attcagtagc
      121 gacgggtcgg agttgaagcg cgtgcgaaag agttgctcgc tgagtatcac gacacggccg
      181 gtcgccgaag gctggtcttc atccggcaga aatgtgcggc ccaatttagg tgcgacaccc
      241 agaagcggaa tgagatcggc agttgcaact gtgccttgca gtcgagccgg ctcgccgcgg
      301 cctgtcatga tgaaatcact gctgtagtag gcggcaacgc gttcgaagct tgtgttctgc
      361 gcgcgcaggt caaagaagtt tggatatgaa tgcgaaccgc gcaaactgcc gcgctgccga
      421 tcagtttcaa acagcgcgac gagtgattcc gaattcggga acggcaaggg ccgcaagagc
      481 acggcgttga cgacgctgaa gatcgcagtg ttggcgccga tgccgagtgc gagcgcgatc
      541 gtggccacga tgctcaaact cggggatttg agcagcatcc gcagaccaaa ctttatgtct
      601 tgaattaatg tttccatccg tctatttcca atttccaatt gccgattgcc aatttttacc
      661 agcggacgac cgactcaaaa atcggcattt gacaatcggc aatcggcaat gcctttactc
      721 gtacctaagc gccaccaacg ggtccacttg cgtcgctcgc ctcgctggta agtaacaagc
      781 caacaaagcc acaactatta aacctgctga aaccagcaag agcatcgtca cgttgcccgg
      841 cgcgacacca aacaacaacg actcgagcaa acgcgagaac gcgatcgagg cgcttacacc
      901 aaccagcacg cctacagcga caagcaccac accgcgaatc atgatcaact tgaaaacatc
      961 acgacgctgc gcgccaaatg ccacgcggat tccgatctcg cgcgtgcgcc gcgccaccga
     1021 aaacgtcagc acgccgtaaa gcccaaccgc cgtgagcaac gtcgccaaca aaccgaacgc
     1081 cgacgagagc cacgtcacca gtcgttcctg gaccagcgac tcgtcaatct ccgtcgcgag
     1141 cgtcttgatg tcgtagagag gcaactgcgg atcgagtgct ttcaactggg tgcgcagcga
     1201 ttcactaact gtcgcgggct cagtttcagt cttcacgtgc agcgtgaggc tggtcagcac
     1261 tttttcctga gctgtaggca ggtaaaagtg gcgccgccgg tcctggcgca ggtttatgta
     1321 ttgcgcgtct ttgacgacac cgacaatggt catgtcggga attttgccga gtccgatccg
     1381 cttcccgagc gggttcgccg ttccgaagaa atggttcgcc atcttttcgt tgatgatgac
     1441 gacgcgcgga cttcccaacc ggtcctcttc gttgaagtcg cggccggcca cgagcgggat
     1501 ttcgagagtt ttgaaatagt cagcggaaac gccagtaaag tcgccgaaca ttcgttcacc
     1561 ttcgcgaggt gtatagcctt cgacaacgac ccggagttga tcccagccac cggagatcgg
     1621 cgagtcagtg gcaagactgg cgtacttcac tcccgggagc gttcgagcac gcgccaacag
     1681 ttcgttgtag aagtttgccg tccgttcagg tgagtatccg ttgagcgctg gattgatgct
     1741 cgcaagcaga acattctctt taccaaaccc ggtgtcgacc gcgcgcaggt tctgcagcgt
     1801 cttgatcagt agtccggcgg tgaatagtaa cgggagtgaa agtccaacct ggaccaggat
     1861 caaagcgcta cgcagactca ccaattttcc tgctgtcgta acactgcgtg attcgagacg
     1921 cagtccgggc gcaaatacct ggcgcgcggc ttgaatcgcg ggcgcaagac tgaacgcaag
     1981 ccctaacaaa acggcgacgc caaaaataaa tgcgtacaaa cgccaatcta atggaccacc
     2041 cagattgacg cgaaagtcgg acggggtgaa cgccatcaat cccgacttta tccacagggc
     2101 tataaccata ccgactgcgc cgccgataaa cgcgagcaca attccctccg tcaaccattg
     2161 gcgcaacagg cgcaatcgtg atgcaccgag cgccagacga accgcagtct cctgcgcacg
     2221 cccagtcgca cgggccatca tcagatttgc gagattcgcg caaagaatca gcagcaccgc
     2281 acacgtcgcg ccaaaaagta accagagcga cgttttgagt tccgtctgca agtggcgaaa
     2341 tccctggtcg ccgggggcga cggcaattct ctcggccacg tacttttcac gatcggatgc
     2401 gctgaggctc gcagccaact cctgcgcttc tgcctcgcga atctgtcgat aaagaacgtt
     2461 gagcgacgct tccgcttgtt ccgaactaac agcggtcttg cgcctcgcca tcaggctcaa
     2521 ccagtggtgg cgccaactct gcaaacgcgt cggcggcaag ggattgaaga ccggtgtcat
     2581 catcatcggc acgcgcacgt cgggatggtc ttgaagattc acaccgtaaa agcccggcgg
     2641 cgtgacgccg atgacggtca acggatgtgc attaaccaga atcgtttttc cgatgagatt
     2701 cggatccttg ccaaaacgac tctcccaata cgcataactc aacactgcga ccggatgcgc
     2761 gcccggcatg cgatcgtcct cttgcgtgaa aagacgtccg gcccaggggc gcacgccgag
     2821 cacttcaaag aagtttccac tgacgagttc gccgcgcacg cgttcagtct gatcgccata
     2881 actgacgttc atctgcgcgc cgccgcgagc gatcactccc gcgaacacat cgtttcggtc
     2941 gcgcagactc tggtacatcg gatacgaaaa cggactgtcg atctgcgggt cacccttcga
     3001 gtaagacgac aacaccgcga gctgttccgg atttgtaacc gccaggcttt gcaac
//
LOCUS       DASTEY010000470         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1460273, whole genome shotgun sequence.
ACCESSION   DASTEY010000470 DASTEY010000000
VERSION     DASTEY010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460273"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..569)
                     /gene="hpt"
                     /locus_tag="VFO72_08255"
     CDS             complement(3..569)
                     /gene="hpt"
                     /locus_tag="VFO72_08255"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET9479321.1"
                     /translation="MATSSAQVPSEFSNPNLEILLTEEQIQARIAELGAQITRDYAEL
                     NPLLIGVLKGACFFLSDLLRQIDTRLGIEFMAISSYGSSTRTSGEVRIMKDLDVPIEG
                     RHILVIEDIVDTGLTLSYLLANLHARGAASVKLAALLDKYERRQKEVRIDYLGFKIPD
                     EFVVGYGLDFAERYRNLPFIAVLKDQEA"
     gene            693..1547
                     /locus_tag="VFO72_08260"
     CDS             693..1547
                     /locus_tag="VFO72_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9479322.1"
                     /translation="MRTLFLIIALAVFAAPVLGQSTAIQPCEATPSTSKAIAASARPS
                     VRSSQTVIDANLPDDDAVEKMLAPYSGKVRALSVVIGRLDAPLKKETVGAGSLGTFVT
                     DGIKASARTKLGKPIALAITNAGGLRKNEIAAGELRATDIFELLPFENALVAVDLTGS
                     DLIKLLPAVVRDAQSGARIQYKWNDQNRPEFISGKLLDENGREQEIDPKKIYTIVTID
                     YLLKVGGGAYAFLKEARSTTPLNITLRDAIIDYVKNETAAGRTIRARAGNRYVQVGPG
                     PKTTEEPR"
     gene            1544..>2069
                     /locus_tag="VFO72_08265"
     CDS             1544..>2069
                     /locus_tag="VFO72_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9479323.1"
                     /translation="MISRRKFLTTSAVFSAAIVTLPRSLAFNADETVITILHTNDTHS
                     QIDPLPPNDKNAGKGGVARRATLVKRIRKENPNTLLVDAGDVLQGTPYFNFYKGEVEY
                     KAMSAISYDAGTLGNHEFDNGVEALAAALKFANFDVVSANYDVKGTVLESKVKRYVVR
                     TLGGIRVGLFGLGVS"
BASE COUNT          513 a          595 c          553 g          408 t
ORIGIN      
        1 atttacgcct cctggtcctt caacacagca atgaacggca aattacgata acgttcggca
       61 aaatccaagc catacccgac gacaaattcg tccggaatct taaaaccgag gtagtcgatc
      121 ctcacttcct tctgccgccg ctcgtattta tcgagcagcg ccgccagctt cacactcgcc
      181 gcgccgcgcg cgtgcagatt cgcgagcaag tacgaaagcg ttaaaccagt gtcgacaatg
      241 tcctcgataa cgagaatgtg ccgtccttca atcggcacgt cgagatcttt catgatgcgc
      301 acctcaccgg aagttcgcgt tgacgatcca taactggaga tcgccatgaa ctcaatgccc
      361 aaccgcgtat cgatctggcg cagcagatcg ctgagaaaga aacaggcgcc tttaagcaca
      421 ccaatcagca acggattgag ttccgcgtag tcgcgcgtga tctgcgctcc caactccgcg
      481 atgcgcgctt ggatctgttc ttccgtcaac aaaatttcga gatttggatt gctaaactcc
      541 gaagggacct gcgctgaaga agtagccaac tcgtttctcc ctctcaaact gcatcgattg
      601 gtcgagcaaa gacgtttgcc tgggtgccgc gtggtgcgac atactatgca agctcttccg
      661 taaggtcaat gcgtcatcgc ccctacaatc caatgcgcac cttgtttctc atcatcgcac
      721 ttgctgtctt cgccgcacct gtgctcggac aatcaacggc aattcaacca tgtgaggcaa
      781 cgccgagcac gtcgaaggcg atcgccgcgt ccgccaggcc cagcgtgcgc agtagccaaa
      841 cggtaatcga tgcaaacctt cccgacgacg acgcggtcga aaaaatgctc gcgccctaca
      901 gcggaaaagt gcgagccctg agcgtggtca tcggcagact cgatgctccg ttgaaaaaag
      961 agactgtagg cgcgggctct ttaggaactt tcgtcaccga cggaataaag gcttccgcaa
     1021 gaacaaaact cggcaaaccg attgcgctcg cgatcacaaa tgccggcggc ttgcgtaaaa
     1081 acgaaatcgc cgcgggtgaa ctgcgcgcga ccgacatctt cgagctgctg ccttttgaga
     1141 acgcgctggt ggccgtagat ttgaccggaa gcgacctgat caagctcttg ccggccgttg
     1201 tccgcgatgc gcagtcgggc gcgcggattc aatacaagtg gaacgatcag aaccggccgg
     1261 agtttattag cgggaaactc ctcgacgaga acgggcgtga acaggagatc gatccaaaaa
     1321 agatttacac gattgttacc atcgattatc tgctgaaagt aggcggcggc gcttacgcgt
     1381 ttctcaagga agcaagaagc acgacgcctc tcaacatcac gctgcgcgac gcgatcattg
     1441 actacgtcaa gaatgaaaca gccgccggac gcacgatacg tgcgcgagcc gggaatcggt
     1501 acgtgcaggt tggaccagga ccgaaaacaa ccgaggagcc gcgatgatca gccgccgaaa
     1561 atttctgact acttccgcag ttttcagcgc ggcaatcgtc acactgccgc gaagcctggc
     1621 tttcaacgct gacgagacgg tcatcacgat tctccacaca aacgatactc actcgcagat
     1681 cgatccgctt ccgcccaacg acaaaaacgc agggaaggga ggcgtcgcgc gtcgcgccac
     1741 gctcgtcaaa cgaatcagaa aagagaatcc gaacacgttg ctcgttgacg ccggcgacgt
     1801 gctccagggc acgccgtact tcaacttcta caagggcgaa gttgagtaca aggcgatgtc
     1861 ggcgatcagt tacgacgccg gcacgcttgg taatcacgaa ttcgataacg gcgtcgaagc
     1921 actggctgcg gctttgaagt ttgcgaactt tgacgtggtc tccgccaact acgacgtgaa
     1981 gggaactgtg ctcgagagca aggtcaaacg ttacgtcgtg aggacgctcg gcgggatacg
     2041 agttggattg tttggtctcg gtgtcagtc
//
LOCUS       DASTEY010000471         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1625175, whole genome shotgun sequence.
ACCESSION   DASTEY010000471 DASTEY010000000
VERSION     DASTEY010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625175"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VFO72_08270"
     CDS             <1..1268
                     /locus_tag="VFO72_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9479324.1"
                     /translation="PLWFSWDRPWLRWVALAYNILLIGAAFAESRFCQLPKGLVISRE
                     FGSRFAMGAETEVRIQIQNASNRPVSLLVKDEYPPQMVLSGMREGRVDIDAQSTATLI
                     YEVKPLRRGRFEFGLTAVRFRSKYRLIWCQTNVTEPVTVKVYPNMRRAREAELKALGA
                     RSVVSSHRKTSWRGEGREFESMRDYVRGDELRHISWTATARRGKLTTRQYQIERDQTI
                     LIALDAGRLMTARIEQETKLDSAVHATLALFSAAARAGDNAGLVVFGRRIKSYLPPSR
                     GRDHIEAALEALYAVEPEMIEPSYPHAFEFIAANSKRRALVVLLTDLVDEEGSKELLT
                     SLHILRPRHLPLVVTIADRDLKAVVQQTPSSVKELFTQSVAEEIIHHREAALRLVESI
                     GGLALDVTAAALAPALLETYLRVKERGLL"
     gene            complement(1260..2228)
                     /locus_tag="VFO72_08275"
     CDS             complement(1260..2228)
                     /locus_tag="VFO72_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET9479325.1"
                     /translation="MPPDRFINQRKNAWQRLEDLLKLLDTASLRRLRREEVRELGRIY
                     RRTASDLAIARAESRDPRLINYLNSLVIRAHGRIYRADAQGGKRIRHFFARELPQTFR
                     RTWRYTLLSFSVFAIFALIGFVGTRYDPEFSELVGVDPSFRELYIETKTPWWESLNDA
                     NQIGATSIMTHNIQVTIYTFAFGATCGIGTLFYLAINGANFASILTLCYRAGFGNDLL
                     TFVVAHGVVELSCIFIAGGAGLLIGSAMIMPGNLSRADALRTRGMEAVRLMVGVAVLL
                     VVAGTIEGFISPALIDPRIKYSIAALTGVTLYAYLLLAGHEKAKSQ"
     gene            2250..3161
                     /gene="ftcD"
                     /locus_tag="VFO72_08280"
     CDS             2250..3161
                     /gene="ftcD"
                     /locus_tag="VFO72_08280"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET9479326.1"
                     /translation="MQRLVECIPNFSEGRRPERVQSIADAISAVESACVLDTHIDPDH
                     NRSVITFVAAPESVVEAAVNAVRRASELIDMRTHLGEHPRLGATDVLPFVPVRGVTMD
                     DCVRIAHEAGAVIAKELSIPVYFYERAARRPERVNLEDVRRGALELLREQITTNPERA
                     PDEGPAQVHESAGAIAVGARPFLIAFNVILRSTDIAIARQIARTIRARNGGLPFVKAL
                     GFRLHSRELVQVSMNLINYEVTGMTAAYDAIRSKADLLGVEIDSAEIVGLVPRNALDR
                     NAAYFSKLENFSDSKILEHQIDQCSSL"
     gene            complement(3158..>3729)
                     /locus_tag="VFO72_08285"
     CDS             complement(3158..>3729)
                     /locus_tag="VFO72_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479327.1"
                     /translation="IGYFEGLSLFERMESGFNLYYTFRDGETAWLYARILNVMHQFSG
                     VTAFSLEPYQIGYENEEGIESGAFWFYRKLGFRPVRREALELTLKEEEKLKTRKGYRT
                     PAATLRTLAESPMIFELNEERRGDWDRFQVRKIGFKAQRDKNVLSIIRNITQAKLGPE
                     EVTYLRRMQKDEELRRAIIKLGATDYTDL"
BASE COUNT          787 a         1071 c         1088 g          783 t
ORIGIN      
        1 ttcctttgtg gttttcgtgg gatcgtccgt ggctgcgttg ggtcgcgctc gcttacaaca
       61 tcctgctgat cggtgctgct tttgctgagt cgcggttctg tcaacttccg aaaggtctcg
      121 tcatctcgcg agagtttggc agccgcttcg cgatgggagc ggagaccgaa gtcagaatcc
      181 aaattcaaaa cgcctctaac cgccccgtct cgttgctagt gaaagacgag tatccgccac
      241 agatggtttt gagcgggatg cgggaggggc gcgttgatat cgacgcacaa agcacagcca
      301 cactcatcta cgaagtcaaa ccactacgcc gcggccggtt tgagtttggt ctaaccgccg
      361 tccgtttccg atcgaaatat cgactgatct ggtgccagac aaacgtaacc gagcccgtga
      421 ccgtgaaggt ctatccgaac atgcgccgcg cgcgcgaagc ggagctgaaa gcactcggcg
      481 cgcgctcggt cgtttcctcg catcgcaaaa cttcctggcg cggtgaagga cgcgaatttg
      541 aatcgatgcg cgactacgtt cgtggcgacg agctgcggca catctcatgg acggcaacag
      601 cgcggcgcgg aaagctcacc acgcgccagt atcagatcga gcgcgatcaa acaatcctga
      661 tcgcactcga cgccggtcgc ctgatgaccg cgcgcatcga acaggaaaca aaactcgact
      721 cagcggtaca cgcaacgctt gcactcttct cagcggcggc gcgcgccggc gataacgcag
      781 gtctcgtcgt ctttgggcgc aggatcaaga gctacttgcc gccgagtcgt gggcgcgatc
      841 acatcgaagc cgcgctcgaa gctctctacg cggttgagcc ggaaatgatc gagccttcgt
      901 acccgcacgc cttcgagttc atcgcggcaa acagcaagcg gcgcgcactg gtcgtgttgt
      961 tgaccgatct ggtcgatgaa gaaggctcga aagagttgct gacgtcgctc cacatcctgc
     1021 gcccgcgcca cttgccgctc gtggtcacga tcgccgacag agatctgaaa gcagtcgtgc
     1081 agcaaacgcc ttcgtcagtt aaggaactgt ttacgcagtc agtggcggaa gagatcattc
     1141 atcatcgcga ggcggccctg cgtttggtcg aatcgattgg cggacttgcg ctcgacgtga
     1201 cagcggctgc gttagctccg gctttgttgg aaacgtattt gcgcgtgaag gaacgtggac
     1261 tattgtgact ttgccttttc gtgaccggcg agaagcaggt aagcgtagag cgtcacgcct
     1321 gtgagcgccg cgatgctgta ttttatgcgc ggatctatca acgctggtga aatgaatcct
     1381 tcgatcgttc ccgcgaccac caacagcaca gccactccca ccatcaaccg caccgcttcc
     1441 atgccgcgcg ttctgagcgc atccgcgcgc gagagattgc cgggcataat catcgcgctg
     1501 ccgatcagca gtccggcgcc accggcaatg aagatgcacg acagttcgac aacgccatgc
     1561 gcgaccacga acgtcaatag atcgttgccg aagcccgcgc ggtaacacag ggtcagtatc
     1621 gaagcgaagt tggctccatt gatagccagg taaaaaagcg tgccgatgcc acaggtggcg
     1681 ccgaaagcga aggtgtagat cgtgacctga atgttgtggg tcatgatcga cgtggcgccg
     1741 atctggttcg cgtcgttcag cgattcccac cacggtgttt ttgtttcgat gtaaagctcg
     1801 cggaaggatg ggtctacgcc gacgagctct gagaactccg gatcgtaacg tgttcccaca
     1861 aagccgatga gcgcgaagat ggcaaagact gagaacgaga gcagcgtgta gcgccaggtg
     1921 cggcgaaacg tctgtggcag ctcgcgcgcg aaaaagtgtc gaatgcgttt gccgccctgc
     1981 gcgtcagcgc gatagatgcg accgtgcgcg cgaatgacca ggctgttcag gtaattgatc
     2041 aggcgcgggt cgcgcgattc ggcgcgagcg atagcgagat ctgaagcggt gcgacgatag
     2101 atacggccca gttcgcgaac ttcctcgcgt cgcaaacggc gcagcgacgc ggtatcgagc
     2161 aacttgagca ggtcctcgag ccgttgccag gcgtttttgc gttggttgat gaagcgatcg
     2221 ggaggcacga cgaggattat aatccaatca tgcagcgatt ggttgaatgt atccctaatt
     2281 ttagcgaagg ccggcggccg gaaagagtcc agtcgattgc cgacgctatc tccgcggtcg
     2341 aatctgcatg cgtgctcgac acgcatatcg atcccgacca caaccgctcc gttattactt
     2401 ttgtggctgc gccggaaagc gttgtcgaag cggcggtgaa cgccgtgcgc cgggcttccg
     2461 aactcattga tatgcgaacg cacttgggcg agcatccgcg gctcggcgcc accgacgtgc
     2521 tgccgttcgt tcccgtgcgc ggcgtgacga tggacgactg cgtgcgtatc gctcacgaag
     2581 caggcgcggt gatcgcaaaa gagctctcga ttccggtcta cttctacgag cgagctgcca
     2641 ggcgaccgga gagagtgaac ttagaagacg tgcgtcgtgg cgcgttggaa ctgcttcgcg
     2701 aacagatcac caccaacccg gagcgtgcgc ccgacgaagg tccggcccag gtccacgaat
     2761 ccgccggcgc gatcgcggtg ggcgcgcggc cgttcctgat cgcgttcaac gtaattctgc
     2821 gcagcactga cattgcgata gcgcggcaga tcgcgcgtac gatccgggcg cgcaacggag
     2881 ggctgccgtt tgtcaaagca ctcggcttca gattgcactc gcgcgaactg gtccaggtct
     2941 cgatgaattt gattaactac gaagttactg gaatgactgc cgcgtacgac gctattcgct
     3001 caaaagccga tctcttaggt gtagaaatcg acagcgctga gatcgtgggc ctggtaccgc
     3061 gaaacgccct cgaccgcaac gccgcttact tctccaaact cgaaaacttc tccgattcta
     3121 agatcctcga acaccagatc gatcagtgtt catctcttta aagatccgtg taatccgtgg
     3181 cccccaattt gataatcgcg cgccgtagct cttcatcctt ctgcatccgc ctgaggtaag
     3241 tcacttcctc tgggcctagt ttagcttgcg tgatgttacg gatgatcgac aacacgttct
     3301 tatcccgttg agctttgaat ccgatcttgc gcacctgaaa cctgtcccag tcgccgcgtc
     3361 gctcttcatt caattcgaag atcatcgggc tctcggcaag cgtgcgcagc gtggccgcgg
     3421 gtgtccggta tcctttgcgt gtcttgagct tttcttcttc tttcagcgtg agttccaacg
     3481 cttctctgcg caccggacga aatccgagct tgcggtaaaa ccagaacgcc cccgattcga
     3541 ttccttcctc gttttcgtag ccgatttgat agggctcgag tgaaaaggca gtgacgccag
     3601 aaaattgatg catgacgttg agaatgcgcg cgtagagcca ggccgtttca ccatcacgaa
     3661 acgtgtagta caagttgaaa ccgctctcca tgcgttcgaa cagcgagagt ccttcgaagt
     3721 aaccgatcg
//
LOCUS       DASTEY010000472         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_405767, whole genome shotgun sequence.
ACCESSION   DASTEY010000472 DASTEY010000000
VERSION     DASTEY010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405767"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="nadA"
                     /locus_tag="VFO72_08290"
     CDS             complement(<1..925)
                     /gene="nadA"
                     /locus_tag="VFO72_08290"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET9479328.1"
                     /translation="MITDATTEEMYQKLKRLLADTMPDFELRLKAELAVEINRLKVDK
                     NAVILGHNYMEPALFHSIPDFVGDSLELSRKAAETDKDIIVFCGVKFMAETAKILNPT
                     KKVLIPSEKAGCSLAASITAEDVRELKRRFPGVPVVTYINTYADVKAESDICCTSSNA
                     VDVVNSLGTDRVIFIPDEYLAGNVARETGKHIIYPTREPRKQFPEDLDYQMIGWPGRC
                     EVHEKFSVEDIKNVRKQFPDVVILAHPECSPEVVAASDYSGSTTAMIRYVEKTRAPRY
                     LLLTECSMGDNIAAANPDKEMLRLCSVRCPHM"
     gene            complement(927..1793)
                     /gene="nadC"
                     /locus_tag="VFO72_08295"
     CDS             complement(927..1793)
                     /gene="nadC"
                     /locus_tag="VFO72_08295"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET9479329.1"
                     /translation="MLKNLPNQAALPVEVSDCIRRALKEDIGSGDVTTNTIVPENASL
                     RGRIIAKQNGVVAGLSVAEAVWLELDNRVTFTPQITDGTRVDTKTVLAEVAGPARALL
                     TGERTALNFLGRMSGIATLTRQFVDAVSSTRAVILDTRKTAPGLRFTDKRAVRTGGGQ
                     NHRTGLFDMILIKDNHIDFAGTITAAVNRVRESGTSLEIEVEARTLAHVREAVSLGVE
                     RILLDNMSLELMREAVRICNGRAKLEASGNVSLDNVLEVARTGVDYISVGALTHSPKV
                     FDVSLEFVESHD"
     gene            complement(1810..3882)
                     /locus_tag="VFO72_08300"
     CDS             complement(1810..3882)
                     /locus_tag="VFO72_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET9479330.1"
                     /translation="MRKAFVLITILLLLAAASAFLLFRLSRTPTPTPVGWRAHVTTLA
                     GDGAPLFRDSQQSAQAAFSDPFGIAVAEDGTVYVADAGESNRIRKITKDGIVSTLAGG
                     SEGFADGTGSGASFNTPSGLALDAEGNLYVADTGNNRIRKVTPLGEVFTIAGDGNAGH
                     VDGPAAQARFNGPIGVAVDARGNVFVADTYNDRIRMISPDGQVSTVAGVFDTPCGVIT
                     TANGTLIVADTGNDRLQRITTDGNVNTFSVSINGEDLSNPVGLALTHDNYLYVTELDR
                     SRVIQVAPDGSTHIIAGRGPGFANGSDSARFNQPAGVAIDPRNGALYVADGANFLVRK
                     LNPTTPSDATTASADSSPESLPRLTNETLALPALLWPLDPQDRQHEVVATIGEVRGSF
                     DSTDSRHHLHSGLDVFGAYGNVVRVVRSEKVTSPLPNWGLNSLNEGLRVGLVSYIHIH
                     VGRDKDAKMFDDPRFMQVTGADGKPARVRIRRGTRFNTGDAVGTVNRMYHVHLNVGPP
                     GGEINPLTLSPIGFKDDVPPTIEKNGIQLFDQSGKQLSEKSNNRLLVSGQVRIVVDAL
                     DRTNMNASRRRLGLYRLGYQLLKPDGTPPPGFEEPRINLLFNRLPPDRESTKIAYADQ
                     SGITVYGSETTKFLYEVTNTVRDGRAAPDLWDTSKVPPGDYVLRIIAADFSGNEAQEG
                     RDLLIAIR"
     gene            complement(3882..4535)
                     /locus_tag="VFO72_08305"
     CDS             complement(3882..4535)
                     /locus_tag="VFO72_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HET9479331.1"
                     /translation="MPKYLDLDTWPRRELFEFYRDFDKPYFNICTRLDVTNLLKELHH
                     HPSVSVALTYHYFALKAANEIEQFRYRLRNGRVLVHDVINGGTTLMLPNETFTLVYFD
                     YADTFAQFIGPAQRAVEEAKNDPAFRPRHDDDARIHCTTLPWISFTSFSHARNWRRED
                     SVPKISFGKFTQENGRTLMPFSVEVHHALVDGLHVGRYVARIEEMLAQPELYLNTSR"
BASE COUNT         1003 a         1319 c         1415 g         1126 t
ORIGIN      
        1 tcatgtgcgg acaacgcaca ctgcacaacc gcaacatctc tttgtcagga tttgcggcag
       61 cgatgttgtc gcccatcgaa cattctgtca acaagaggta acgcggcgcg cgtgtcttct
      121 cgacgtaacg aatcatcgcc gtggtgctgc ctgaataatc cgaagcagca acaacttccg
      181 gactgcattc cggatgggcc agaatcacta cgtcaggaaa ctgtttacgc acgttcttta
      241 tgtcttcaac actgaacttc tcgtgcactt cacaacggcc cggccagccg atcatctggt
      301 agtcgagatc ctcgggaaac tgtttgcgcg gttcgcgcgt tggataaatt atgtgcttcc
      361 ctgtttcgcg tgcgacattg cccgcgagat actcgtcagg aataaaaatc actcgatctg
      421 ttcccagcga attgaccaca tcaactgcat tggacgacgt gcagcagatg tcagactccg
      481 ctttcacgtc cgcataggtg ttgatgtaag tgacgaccgg cacacccgga aagcgcctct
      541 tcaactcgcg tacgtcctcc gcggtgatgc ttgcagccag agagcagcct gctttctccg
      601 aaggtatcag caccttcttg gtcggattga ggatcttcgc cgtctcggcc ataaacttca
      661 caccgcaaaa tacgatgatg tccttgtcgg tttccgccgc tttgcgactg agttcaagcg
      721 agtcgccgac gaagtcggga atcgaatgaa agagcgccgg ctccatgtag ttatgaccga
      781 gtatcacggc gttcttatcg actttcaatc gattgatttc caccgccagc tcggctttga
      841 gacgcagctc gaagtcgggc atggtgtcgg ccaacagccg tttcaacttc tggtacatct
      901 cttcggttgt ggcatcggta atcatttcaa tcatggctct caacaaactc caaactcacg
      961 tcaaaaactt tcggcgaatg cgtcagagcg ccgacggaga tgtaatccac tcccgtgcgg
     1021 gctacttcga gcacattgtc gagcgatacg ttgccagacg cttccagctt tgcgcggccg
     1081 ttgcagatcc ggaccgcctc acgcatcaac tctaacgaca tattatcgag caaaatcctc
     1141 tcaactccga gcgagacggc ctcgcgaacg tgtgcgagcg tgcgcgcttc gacttcaatt
     1201 tcgaggctcg tgcccgactc gcgcactctg ttcactgctg cggtgatcgt gccggcgaag
     1261 tcgatgtggt tgtcctttat taagatcatg tcaaacagac cggtgcgatg gttttggcca
     1321 ccgccagtgc ggactgcccg tttgtcagta aagcgcaggc cgggcgcggt cttgcgcgtg
     1381 tcgagaatga ccgcgcgcgt actggaaacc gcatcgacaa actggcgtgt gagtgtagcg
     1441 atgcctgaca tgcggccgag aaaattcagc gcggtgcgtt cgccggtgag cagggcgcgg
     1501 gctggtccag cgacttcggc gagaacagtt ttcgtgtcga ctcttgttcc gtcggtaatt
     1561 tgcggcgtaa acgtgacccg gttatcgagt tcgagccaga cggcttccgc aacgcttaga
     1621 ccagcgacga ctccgttttg tttggcgatt attcggcccc gaagcgaagc attttccgga
     1681 acgattgtgt tggtggttac gtcgccgctg ccgatgtctt ctttaagcgc gcgccgaatg
     1741 cagtctgaga cttcgacggg caaggcggct tgatttggga ggttcttgag caaagctcat
     1801 tctaagctct tagcgaatcg cgatcaaaag atcgcggccc tcctgcgctt cgtttccgga
     1861 aaagtctgcg gcgatgatgc gcaacacgta gtcacctggc ggcacctttg atgtgtccca
     1921 aaggtctggc gcggcgcgac cgtcccgcac ggtattggtc acttcgtaga gaaactttgt
     1981 ggtttcgctg ccgtagacgg tgatgccact ctggtccgcg taggcgatct ttgtgctctc
     2041 tcggtctggt ggaagccgat tgaataacag gttgatccgt ggttcttcaa agcctggtgg
     2101 gggagttcca tcgggtttga gaagctgata accgagacgg tagagaccga gccggcggcg
     2161 gcttgcgttc atgtttgtgc gatcgagtgc gtcgaccacg atgcgcacct ggccgcttac
     2221 cagtagtcga ttgttgctct tctccgagag ttgtttgccc gactgatcga acagttgaat
     2281 accgttcttt tctatcgtgg gcggcacgtc gtctttgaag ccgattggcg agagtgtcaa
     2341 agggttaatc tcgccaccgg gtggaccaac gttcaggtga acatgataca tgcggttaac
     2401 tgtgcccacc gcgtcgccgg tattgaagcg cgttccgcgt ctgatcctga cccgcgctgg
     2461 ttttccgtcc gcgccggtta cctgcatgaa acgcggatcg tcaaacatct tcgcgtcttt
     2521 gtcgcgaccg acgtgaatat gaatgtagga gacaaggccc acgcgcaaac cttcgttgag
     2581 cgaattaaga ccccagtttg gcaaagggct ggtgactttc tcggaacgaa ctacacgcac
     2641 gacgttgccg taagcgccga agacgtcgag cccgctatga agatgatggc ggctgtcagt
     2701 tgagtcgaag ctgccgcgga cttcgccgat ggtagcgacg acttcgtgct gccggtcttg
     2761 cggatccagc ggccagagta aagctggcag cgctaaggtt tcgttcgtga gtcgcgggag
     2821 tgattcgggt gacgagtctg cggaagccgt cgttgcgtct gaaggagttg tcgggttcag
     2881 tttgcgaacc agaaagtttg cgccgtcggc aacgtagagt gctccgttac gtggatcgat
     2941 cgcgacgccg gcgggctggt tgaaacgggc gctgtcggat ccgttcgcga agccggggcc
     3001 acgtccggcg atgatgtgag ttgaaccatc cggcgcgact tgaatcacac gcgagcgatc
     3061 gagctccgtc acgtacaaat aattgtcgtg tgtcaacgcg agaccgacag gattggagag
     3121 gtcctcgccg ttaatgctca ccgaaaaagt gttgacgttg ccgtcggtag ttattctttg
     3181 cagacgatcg ttacctgtat ccgcgacgat cagtgttcca ttcgcggtcg tgatgactcc
     3241 gcacggcgtg tcaaaaacgc cggcgaccgt gctgacctga ccatcaggcg agatcattcg
     3301 gatgcggtcg ttgtaagtat cggccacgaa aacattgccg cgcgcgtcga ccgcaacgcc
     3361 gattggacca ttaaagcgtg cctgcgcggc tggaccatct acatgtccgg cattgccgtc
     3421 accggcgatt gtgaatactt cgcccagcgg cgtgacttta cgaatacgat tgttgccggt
     3481 gtcggcgacg tagagatttc cctccgcgtc aagcgcaagg cctgacggcg tgttaaacga
     3541 ggcgccgcta cctgtaccat ccgcgaatcc ttcactgccg ccggccaacg tagaaacgat
     3601 cccgtccttc gtgatcttgc gaatgcggtt gctctcgccg gcgtcggcta cgtaaaccgt
     3661 tccgtcttcg gcgaccgcga ttccaaacgg atcggagaat gcagcttgcg cggattgttg
     3721 gctatcgcga aacagcggcg cgccatcacc ggctaacgtc gtgacatgcg cccgccaacc
     3781 gacgggcgtc ggagttggtg ttcgactgag acgaaaaagt aagaacgcgc tcgcggcggc
     3841 gaggagcagc aggattgtta tcaggacgaa cgctttacgc attaacggga cgtgtttaga
     3901 tacaactccg gctgcgcgag catctcttca atgcgcgcga cgtagcgtcc gacgtgcaaa
     3961 ccgtcgacga gtgcgtgatg cacctcgact gaaaacggca tcagcgtgcg gccgttttcc
     4021 tgtgtgaact tcccgaagga gatcttcggc acggaatcct cgcggcgcca gttgcgtgca
     4081 tgagaaaagc tggtgaagga gatccacgga agggttgtgc aatgtatgcg tgcatcgtcg
     4141 tcgtgtcgtg gccggaatgc tgggtcgttt ttcgcttcct cgacggctct ctgtgctggt
     4201 ccgatgaact gtgcgaacgt gtctgcgtag tcaaagtaaa ccagcgtgaa ggtctcgttt
     4261 ggcagcatca acgtagtgcc gccgttgatg acgtcatgca ccagcacccg gccgttgcgc
     4321 aagcggtagc gaaactgttc gatttcattc gctgctttca acgcgaagta gtgataagtc
     4381 agtgcaacgc tgacactggg gtgatggtgt agctctttta agaggttagt cacatccaga
     4441 cgggtacata tattgaagta cggtttatcg aagtcacgat agaactcgaa gagttcacga
     4501 cgtggccagg tgtcgagatc caggtatttt ggcatgtgcg ggattgtacc tgaaggcggt
     4561 tgcgggcgac gtatcgagtg agttttgctt gtggactttc ccgcattcct gcggaacatt
     4621 ccccagacca gctctcgaca ccagtcattt ttcgcgtaaa agacggtggc atgccctttg
     4681 cacttctccg aaatagttac cgtgcccatc cgtgcaacga gccacttgct cgatattggc
     4741 tagtcacccc caacgatcga gcgaggagtg agcgaatggc ttcgtgcaaa ggataccaac
     4801 gtaacgaccg gcgatgccca gttagcggct tgctaatgca gacggcacag tcggaggtgt
     4861 aga
//
LOCUS       DASTEY010000473         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1592426, whole genome shotgun sequence.
ACCESSION   DASTEY010000473 DASTEY010000000
VERSION     DASTEY010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592426"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFO72_08310"
     CDS             complement(<1..233)
                     /locus_tag="VFO72_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479332.1"
                     /translation="MKATKRKWFSFSFCAFCAFLWLSSAHAQVAPSDYVRTEAMIPMR
                     DGVRLFTQIDAPKNQSSPLPILLLRTPYGLGSIT"
     gene            complement(233..>2707)
                     /locus_tag="VFO72_08315"
     CDS             complement(233..>2707)
                     /locus_tag="VFO72_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9479333.1"
                     /translation="DAVVYEQAGYIYLVDAKSGKPQKLNIEVTGDLPWARPQFKKVAG
                     MIRAATLSPTGTRAAFEARGEIFTVPAEKGDFRNLTQSAGAHDRGPVWSPDGTHVAWL
                     SDASGEYQLMIGEPTGVTPPRAIALPANAFYRGASWSPDGTQILLEDNHNTLWAIEVT
                     SGTATKIDTDNYPDPIRLFNAAWSPDSKWITYSKNLANRLRAVFVYSWPDKKAYQVTD
                     GLADSISPTFDAGGKYLYFMASTNYGPSTGWLEMSSLDRPVRRAIYLVVLSANDPSPL
                     LPETGDEPKPPGPETPQPKPDPNAKPSVRIDLNGISQRILAINIPAGDYNNLNAGAAG
                     TFFYTEPFTGGPPGTLRLQRFQLRERAAAPFLEGSIRTYSISADKKKLLYQAGDGANR
                     WGVVATERPAKVGDGPLNVAQLETRVDPRAEWAQIYRETWRIQRDYFYDPKMHGADWQ
                     AIYDKYKPLLAFVGHRADLGYLIATMGGELTVGHSYLNGAGDVPSEDPVPVGLLGADF
                     AIENGRYRIKRIYTGENWNPELRAPLSAPGIQVAEGDYLLEVNGRPIAPPANVYSFFE
                     GTANRQTLIRVNKAPSLEGSRLITVVPVASEDTLRTRAWIENNRRMVDKLSNGKLAYV
                     WLPNTGNPGYTYFTRYFYSQQDKEGAVIDERYNQGGMVADYIVNELDRKVMGYFALRD
                     GNPTTSPIAGIYGPKVMLINESAGSGGDALPYYFRLRKVGPLIGTRTWGGLVGTLGIP
                     PTIDGGGITAPSLAFYNLKGEWDVENIGVAPDIEVDYTAAEVIKGRDPQLERAVQEAL
                     RLLELNPTRRVPRPAPIDRVSKGSSR"
BASE COUNT          545 a          784 c          770 g          608 t
ORIGIN      
        1 gtgatactgc caagaccgta tggggttctt aacaacagga tcgggagcgg actgctctgg
       61 tttttgggag cgtcgatctg cgtgaacaaa cgcacgccgt cgcgcatcgg gatcattgct
      121 tcagtgcgca cgtagtcgga aggcgctacc tgcgcgtgag cagaagatag ccacaaaaag
      181 gcacaaaagg cacaaaaaga gaaggaaaac catttgcgtt ttgtggcttt cattatcggc
      241 tactaccctt cgacacgcga tcgatcggag ccgggcgcgg cacgcgtcgt gtcggattga
      301 gctccagcaa ccttaacgct tcctgcaccg cgcgttccag ttgcggatcg cgtcctttga
      361 tcacttccgc ggcagtgtaa tccacctcga tgtccggcgc gacgccaatg ttttctacgt
      421 cccactcgcc tttcaggtta tagaacgcga ggctcggcgc cgtaattcca ccgccgtcta
      481 tcgtcggcgg gattcccagt gtgcccacta gtccgcccca ggttcgcgtt ccaattagtg
      541 gtccaacttt tcgcaaccgg aaatagtacg gcagcgcgtc tccaccggag cccgccgact
      601 cgttgatcag catcaccttc ggcccgtaga tacccgctat tggcgacgtc gtgggattgc
      661 cgtcacgcaa ggcgaagtat cccattactt ttcgatcgag ctcattgacg atgtagtcag
      721 caaccatgcc gccctggttg tagcgttcgt caatcaccgc gccttctttg tcctgctgcg
      781 aataaaagta acgcgtgaag taggtgtaac cgggattccc ggtgttgggc aaccacacgt
      841 acgcgagttt accgttcgag agtttgtcga ccatgcgacg attgttttcg atccatgctc
      901 tggtccgaag agtgtcttca ctggccacgg gtacgacggt gatcaaacgc gacccttcga
      961 gagaaggcgc cttgttaact ctgatcaagg tctgccggtt cgcagttcct tcaaagaagc
     1021 tgtagacatt tgcgggtggc gcaatcggac gtccattgac ctccagcagg taatcacctt
     1081 cagccacttg aataccgggt gcgctgagcg gagcgcgcaa ttccgggttc caattctcgc
     1141 ccgtgtaaat gcgcttgatc cggtaccgtc cattctcgat cgcaaaatcc gctccgagca
     1201 gaccgactgg caccggatct tcactcggca cgtcgcccgc tccgttcaga taagagtgac
     1261 cgacggtgag ttctccaccc atggtcgcga tcaaataacc gaggtccgcg cgatggccga
     1321 caaaagcgag cagtggtttg tatttgtcat aaatcgcctg ccagtcggcg ccgtgcattt
     1381 tcggatcgta gaagtagtca cgttgaatgc gccacgtttc gcgatagatc tgcgcccact
     1441 cagcgcgcgg atccacgcgc gtctcgagtt gggcaacgtt caatggtcca tcgcctacct
     1501 ttgcgggccg ctcagtagcg accacgcccc aacgattcgc gccatcacca gcctgataga
     1561 gcagcttctt cttgtctgcg gaaatcgaat aagtacgaat gctgccttcg agaaacggcg
     1621 ccgccgctcg ctcccgcagt tgaaaacgtt gcaggcgcag tgttccggga ggtccaccgg
     1681 taaacggctc cgtgtagaag aatgttcccg ccgcgccggc attaagattg ttgtaatcac
     1741 ccgcgggaat gttgatggcg agtattcgtt gactgattcc gttcagatcg atgcgcactg
     1801 aaggtttcgc attcggatcg ggcttcggtt gcggagtctc agggcctggt ggcttaggct
     1861 cgtctcctgt ctcgggcaag agaggtgacg gatcgttcgc actcaacacc accaggtaga
     1921 ttgcgcgacg aacaggtcga tccagtgagc tcatctccaa ccaaccggtg cttggaccat
     1981 agttggtgct tgccatgaag tagagatact tcccgcccgc gtcgaaagta ggtgagatgg
     2041 agtcggcgag gccgtcagtt acttgatatg ccttcttgtc aggccacgaa taaacaaaga
     2101 ccgcgcgcaa gcgattggca aggttcttcg aatatgtgat ccactttgaa tcgggcgacc
     2161 aggcggcgtt gaatagcctg atcggatcgg gatagttgtc ggtatcgatc ttcgtggcag
     2221 taccgctggt cacctcgatg gcccaaaggg tgttgtgatt atcttccagc aggatctgcg
     2281 tgccgtccgg cgaccagctc gcgcccctgt agaacgcgtt agcgggcaaa gcaatcgccc
     2341 gcggcggagt gacgcctgtc ggctcgccga tcatgagttg atactcaccg ctcgcatccg
     2401 agagccatgc cacgtgtgtg ccgtcaggcg accacaccgg gccgcgatcg tgcgcgcccg
     2461 cgctctgcgt caggttgcgg aagtcgccct tctctgccgg caccgtgaag atttcaccgc
     2521 gagcttcgaa tgcagcgcgc gttccggttg gggagagtgt ggcggcgcga atcatgccgg
     2581 ccaccttctt gaactgcggt cgggcccacg gtagatctcc ggtcacttcg atgttgagct
     2641 tctgcggttt gccggatttg gcatcgacca aatagatata acctgcctgc tcgtacacga
     2701 ctgcatc
//
LOCUS       DASTEY010000474         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1477302, whole genome shotgun sequence.
ACCESSION   DASTEY010000474 DASTEY010000000
VERSION     DASTEY010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477302"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFO72_08320"
     CDS             complement(<1..947)
                     /locus_tag="VFO72_08320"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9479334.1"
                     /translation="MTMFVSLKPRLIRSFQIPALLMVLLLSAHAAFGQAQANAADIQG
                     LVKDSTGAVVANAAVTARNPATNFTRAAITNDEGLYRIINVPPGDYEITVEATNFKKA
                     VLPKVTVTIGQAAQIDDITLEPGQVSEIVTVSDATAEIVETSKTAVATTIDQQRINNL
                     PINERNYINFALTTSTVSRDAGRPIGPAPTTGLNFGGQRGRSNLVQVDGADNTDNSVN
                     ASRSTVSQEAVQEFQVVTNSFAAEFGRSAGGVVNVVTKSGTNELHGNIFGFLRHRSFQ
                     ARNAFAPIEDPPFTRTQYGFTIGGPFDKDRTFFFFAFEQR"
     gene            complement(1108..2277)
                     /locus_tag="VFO72_08325"
     CDS             complement(1108..2277)
                     /locus_tag="VFO72_08325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9479335.1"
                     /translation="MDHANTFDVIIAGAGPAGSSAAIHLARRGVRVLLVEQKKFPRAK
                     LCGEFISPECQKHFENLGVAESMSASQPAAITETVFYSSGGHHITVPSSWFGGPAALG
                     LSRAVMDHVLLRRAQDCGVTVMEGTTVTEPILHATEVRGVRVKETEYLAPLTIDATGR
                     ARILTKKLHRSEPKSKPKLIAFKAHLRNTRVAPNACEIYFYPDGYGGLSTVEGEISNL
                     CFIISAEQVKRHYSNPEVVVREMVMKNRRAAYTLEHAQPVSEWLSASWERFGRQQPCP
                     ARGLLTIGDSASFIDPFTGSGMLMALESGELVADVIVRNRKAPSLVAAEYASEYIQKF
                     DSRLRISGWLRRAAFNPRLAGLGLSICGASKHFRSRIARATRSPKRDHHGPLRAG"
     gene            complement(2377..3135)
                     /locus_tag="VFO72_08330"
     CDS             complement(2377..3135)
                     /locus_tag="VFO72_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9479336.1"
                     /translation="MFQKFKQRSHRLEYIDTGNYTAEEYEGCIGELQFVNRWMGDAHS
                     LKTTLLREIEEQGLRSFSVLDVGAGSGELLRVATVWARQTGRQLRAVGLELNERMAES
                     INEGSDRFDEISSVRGDALTLPFSEAQFDYVICSLFTHHFVDEQVVQILREMSRVAKR
                     RIFVIDLHRHPIAYLLYTTLGKLVLHNRLLRHDGALSILRSFKVDELNELAQRAGLRD
                     IWVERHFPYRLVLNAAARATVHLQEPEVRAHRAA"
     gene            complement(3148..>3466)
                     /locus_tag="VFO72_08335"
     CDS             complement(3148..>3466)
                     /locus_tag="VFO72_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HET9479337.1"
                     /translation="LNDRIETSALKLAFDGQAARIPMSATKSQIGHPQGASGAAGLSA
                     ALCAMHTNKIPPTINLDEPDPDCDLDYVPNEAREASVRIALCNCIGFGSKNSALVVQK
                     LDR"
BASE COUNT          732 a          974 c          985 g          775 t
ORIGIN      
        1 cgttgttcga aagcgaagaa gaagaacgtt cggtccttgt cgaatggtcc accgatggtg
       61 aatccatatt gcgtgcgcgt gaacggtgga tcttctatcg gcgcaaaagc gttgcgcgcc
      121 tggaagctgc ggtgacgcag aaagccgaag atgtttccgt gcaactcatt ggtgcctgac
      181 ttggtaacaa cattcaccac gccaccggca gagcggccaa actcagcagc aaacgaattg
      241 gtgactacct gaaactcctg cactgcttcc tgactgacag tggagcgcga tgcgtttacg
      301 gagttgtccg tgttgtcggc gccatcaacc tggaccaggt tggagcgacc acgctggcca
      361 ccgaagttca agcccgtcgt aggcgctggt ccaataggac gacctgcgtc gcggctgaca
      421 gtggaagtgg tcagcgcaaa gtttatgtag ttgcgttcgt tgattgggag gttgttgatt
      481 cgctgctgat cgatcgtcgt cgcgacagcg gttttggaag tctcaactat ttccgcggtc
      541 gcatcggaaa cagtgacgat ttcggaaacc tgacccggct cgagcgtaat gtcgtcaatt
      601 tgcgccgctt gcccgatcgt aaccgtgact tttggcaaaa cggctttttt gaagttcgtc
      661 gcctcaacgg ttatttcata gtcgccgggt ggcacgttga tgattcgata aagaccttcg
      721 tcgttagtga tggcggcgcg cgtgaagttc gtggccggat tgcgtgcggt cacagcggca
      781 tttgctacga ctgcgccggt ggagtctttt acaagaccct ggatgtcggc ggcgtttgcc
      841 tgcgcttgtc cgaaagccgc gtgggcagag agaagcagga ccattaacaa agcggggatt
      901 tgaaatgatc tgataagccg gggcttcaac gatacaaaca tcgtcatttc tccttagttg
      961 ttttttgcgg ctctggcatt cgcgcgtcac aaaacccttg gtttacgttc gctttgccag
     1021 tggacatgaa tcgtgggagc aacagtgaca gagagagcag cactggctga gcctttgtac
     1081 tctaaaagag tcaacgtggc aagcagttta acctgcgcgc aaggggccgt gatggtcgcg
     1141 ctttggggag cgagtggctc gcgcaattcg acttctaaag tgtttgctgg cgccgcagat
     1201 ggatagaccc aggccggcta gacgtggatt gaaagccgcg cgtcgcagcc agccggaaat
     1261 ccgcaggcgt gaatcaaact tttggatgta ctcggaagcg tattcagcgg ctactaatga
     1321 aggtgctttt ctattacgaa caatcacgtc cgcaaccagc tctccactct cgagcgccat
     1381 tagcatgccg ctgccggtga aaggatcgat gaacgacgcc gagtctccga tggtgagcag
     1441 gcctctggca ggacacggct gttgccggcc gaagcgttcc cacgaggcac tcagccactc
     1501 cgatactggt tgtgcgtgct cgagcgtgta agcggcgcgc cggttcttca ttaccatctc
     1561 gcgcacgacg acttcggggt ttgaataatg gcgcttaact tgttccgcgg agatgatgaa
     1621 gcagagattg ctgatctcgc cttcgacggt gctcaggcct ccgtaaccgt ccgggtagaa
     1681 gtagatctcg cacgcgttgg gcgcgacgcg tgtgttgcgc aggtgcgctt tgaaggcgat
     1741 caactttggt tttgattttg gttcgctgcg gtggagtttt tttgtgagga tgcgcgcgcg
     1801 gccggtggcg tcgattgtga gtggcgcgag gtattcggtt tctttcaccc tcacgccgcg
     1861 tacttcagtc gcgtgtagga tcggttcggt gacggttgtg ccttccataa cagtgacgcc
     1921 gcagtcctgt gctcttcgga gcaacacgtg gtccatgacg gcgcggctca ggccgagcgc
     1981 cgcgggcccg ccgaaccaac tgcttgggac tgtgatgtga tgaccgccgg aggagtagaa
     2041 gacggtttcc gtgatcgcgg cgggttgcga ggctgacatg gattcggcga cgccgagatt
     2101 ctcgaagtgt ttctgacact caggagaaat aaactcaccg cacaactttg cccgcggaaa
     2161 cttcttctgc tcgacgagca acacgcggac accgcgacgc gccagatgaa tcgcagcact
     2221 cgaccccgcc ggccccgccc ccgcaatgat cacatcgaat gtgtttgcgt gatccatgtt
     2281 tcccagcacg ggcggccacg gagtgccgcc cctacaataa cgcccggatc ttatttgtag
     2341 gggcggcaca ccgtggccgc ccgtcgtccg catctcttat gccgctctgt gtgcgcgcac
     2401 ttcaggttcc tgtaaatgca cggtggcgcg ggcggcggcg ttcaggacca ggcgatatgg
     2461 gaaatggcgc tccacccaaa tatcgcgcag cccggcgcgt tgggccaact cattcaactc
     2521 atcaactttg aaacttcgca gtatcgacaa cgccccatcg tgtcgcaaca accgattgtg
     2581 caaaaccaat ttcccaagtg tcgtgtaaag cagatacgca atcggatgcc ggtgaagatc
     2641 gataacaaat attcgccgtt tcgccacgcg actcatctca cgaagaatct gcaccacctg
     2701 ctcgtcaaca aagtgatgcg taaacagcga acagatcacg taatcaaact gcgcctcgga
     2761 aaacggaagc gtcaacgcat cgccacgcac cgagctgatc tcatcaaaac gatccgaccc
     2821 ctcgttaatc gactccgcca tgcgctcgtt taactccagc cctaccgcac gcaactgtcg
     2881 accagtttgc ctggcccata ccgtcgctac gcgcagcaat tctccagaac cagctcccac
     2941 atccagcacc gaaaagctcc gcaacccctg ctcttcgatc tcgcgcaaca gcgtggtctt
     3001 cagactatgc gcgtccccca tccagcggtt tacaaactgc agctccccaa tacaaccctc
     3061 atactcttcc gccgtgtagt taccggtgtc gatgtattcg agccggtggc tgcgctgctt
     3121 aaacttttga aacatatgga ccactgatca acgatcgagc ttttggacca ccaacgcact
     3181 gttcttcgag ccaaagccaa tacaattgca gagagcaatt cttacgcttg cttcacgggc
     3241 ttcgttgggg acgtagtcga gatcacaatc agggtcaggt tcgtcgagat taatcgtggg
     3301 cgggatcttg ttcgtatgca tggcgcagag cgcggcgctc agaccggcag cgccgctcgc
     3361 gccctgcgga tgtccgattt gtgacttcgt agccgacatc ggaatgcgtg cggcctgacc
     3421 gtcaaacgcg agcttcaaag cactggtctc gatacgatcg ttgagc
//
LOCUS       DASTEY010000475         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_752148, whole genome shotgun sequence.
ACCESSION   DASTEY010000475 DASTEY010000000
VERSION     DASTEY010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752148"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..2105
                     /locus_tag="VFO72_08340"
     CDS             36..2105
                     /locus_tag="VFO72_08340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9479338.1"
                     /translation="MFRRRLNCLSLLLILSFAFSLTAQTARRPIKLDDMNRFRNVGDP
                     QISPDGKWVAYVVNTQNAKEDKSNSHIWMVGIDGSNDRQITFSNESENSPRWSPDGKY
                     LSFTSSRPGKTRGNQVWLLDRNGGEAQQLTEIKGRLQGHEWSPDSKRLALVIGDPDPD
                     ADPNPSPQPGATPRVPKPIVIDRYRFKQDGAGYLLRGRNTYIYLFDLETKKLDRLTKS
                     KWDEGSPAWSPDGTRIAFMSNHIEDPDRDPAAQLFVAEAKPGATEKQLTPEGARAGRS
                     RPEWSPDSKWIAFLEGEEKKYGAYSMDHLALVPSDGSSAPTRFKATEDLDRGVSSPRF
                     SPDGKSIRFLVTDDRSVYPMKANWSGGAAEKLLSPPIVISSWNFGAGKAVAISGGDNK
                     ANEIHVWEGNSLRQLTHQNDELFAELDLGVTEEVGFKSKDGTQVNGLLTYPTGYMKGT
                     RVPLLLRIHGGPNAQDQHSFSLERQMFAAHGYAVLAVNYRGSSGRGQKFSRAIFADWG
                     NYEVQDLLAGVDHVIKMGVADPDRLGVGGWSYGGILTDYLIATDNRFKAATSGAGTAF
                     TVAFYGTDQYIIQYDHEIGPPWNPKAWETHVKISYPFLHADRILTPTLFMGGERDFNV
                     PIEGSQQMYQALRSLGIDTQLIIYPNENHGIQRPSYQRDRMERYLAWYEKYIRKAAGP
                     SSTSTAK"
     gene            2127..2873
                     /locus_tag="VFO72_08345"
     CDS             2127..2873
                     /locus_tag="VFO72_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9479339.1"
                     /translation="MRLFATSDLHSDYKENFNWLKQLSDTAYRDDALIVAGDVSDRLE
                     IVRETLLLLRAKFRHVLFTPGNHELWVRGEPFDSIEKLKRILDLCEELDVTTRPLRLD
                     DVWVVPLFSWYDGVFNSEKDERTARLSWVDFHVCKWPEDAAPLPDYFLRMNEPHLKTY
                     DAPVVTFSHFIPRSDIFPPKEYLRFSWLESVSLCAGLDHQIRTLKSHTHVCGHTHTTF
                     DLVIDGVRYIQNAVRYPRERTETSVPIKQI"
     gene            complement(2959..>3727)
                     /locus_tag="VFO72_08350"
     CDS             complement(2959..>3727)
                     /locus_tag="VFO72_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479340.1"
                     /translation="RLEQIIWNLLNNSVKFTPRGGRIEVRLDEDGEDIVLTVADNGQG
                     IDTSFLPHIFEIFRQADSGTNRAQSGMGIGLAVVQQLVELHGGSVAAYSAGVGQGAMF
                     TIRLPRSVDTKRRPSLSMDLTIGTLQGMEVLVVDDSEDTTEMVRHLLEIGGASVWAAT
                     SGAEALRFAREREFDVVLSDISMPEMDGFEFLSRLRDLPGKQDLPAVALTGFGRPEDV
                     QRASDQGFYAHLTKPFDLQTLARLLQKIPRRNGHKEA"
BASE COUNT          838 a         1103 c         1010 g          776 t
ORIGIN      
        1 gcgcccgatc taaaaccaaa attcaggagg cccctatgtt ccgaagacgc ctgaactgtc
       61 tttcgctgct actgatcctt tcgtttgctt tctcgctcac ggcgcaaacc gcacggcgtc
      121 caatcaaact cgacgacatg aaccgctttc gcaacgttgg cgatccgcag atctcacccg
      181 acggcaaatg ggtcgcgtac gtcgtcaaca cgcagaacgc gaaggaagac aaatcgaatt
      241 cccacatctg gatggtgggc atcgacggca gcaacgatcg acagatcact ttcagcaacg
      301 agagcgagaa ctcgccgcgc tggagccccg acggcaagta tctctctttc acttcttcgc
      361 gaccgggcaa gacgcgcggc aatcaggttt ggcttctcga tcgcaacggc ggcgaagcac
      421 aacagctcac cgagatcaaa ggacgtctgc agggacacga atggtctccc gattcaaaac
      481 ggctggcact ggtgatcggc gatcccgatc cggacgcgga cccaaaccca tcgccacaac
      541 ccggcgccac accgcgcgtt cccaagccga tcgtgatcga tcgctaccgg ttcaagcagg
      601 acggggcggg atatctgctg agagggcgta acacgtacat ctatctcttc gatctcgaaa
      661 ccaagaaact cgaccggctc accaagagca aatgggatga gggctcgccc gcatggtcgc
      721 ccgatggaac tcgcatcgcc ttcatgagta accacattga agatcccgat cgcgatcctg
      781 cagcgcaact ctttgtcgcc gaagcgaaac ccggcgcgac cgagaagcag ttgacgccgg
      841 aaggcgcacg cgccggacgt tcgcggcccg agtggagtcc cgactcgaag tggatcgctt
      901 ttctcgaagg cgaggagaag aagtacggcg cttacagcat ggaccatctc gcgctggtgc
      961 cgtctgacgg cagcagtgct cccactcgtt tcaaggcgac ggaggatctc gatcgtggcg
     1021 tctcttcgcc gcgcttcagt ccggacggca aatccattcg ttttctggtc actgacgatc
     1081 gctccgttta tccgatgaag gccaactggt ccggcggcgc cgcggaaaaa ctactctcgc
     1141 caccgatcgt gatttccagt tggaattttg gcgccggcaa ggcggtggca atctccggcg
     1201 gtgacaataa ggccaacgag atccacgttt gggaaggaaa ctcgcttcgc cagcttacac
     1261 accagaacga cgagcttttc gcggagttgg atctgggagt cacagaagag gtcgggttca
     1321 agagtaaaga cggcacgcag gttaatggtt tgctgacgta ccccaccggc tacatgaaag
     1381 gaactagagt tccactgctg ctgcgaattc acggtggtcc aaacgcacag gaccagcact
     1441 cgttcagtct cgagcggcag atgtttgcag cccacggtta cgccgtgctg gcagttaact
     1501 atcgcggcag ttccggtcgt ggacaaaagt tctcgcgcgc gatctttgcc gactggggca
     1561 actacgaagt tcaggatcta cttgccggag tcgatcacgt gatcaagatg ggcgtcgcag
     1621 atcccgatcg gctcggcgtg ggcggctgga gttatggcgg catcctgacc gattacctga
     1681 tcgcgactga caatcgattc aaagcagcta ccagcggcgc tggcacggcc ttcacggtgg
     1741 ctttctacgg caccgatcaa tacatcattc aatacgatca cgagattggt ccgccttgga
     1801 atccgaaagc gtgggagacg cacgtgaaga tctcgtaccc gttcctgcac gcggatcgta
     1861 ttctgacgcc gacacttttt atgggcggcg aacgcgactt caacgttcct attgaaggca
     1921 gtcagcaaat gtaccaggcg ctgcgcagcc tcgggatcga cacgcagctg atcatttatc
     1981 caaacgaaaa ccacggcatt caaaggccga gctatcaacg cgatcggatg gagcgatacc
     2041 tggcgtggta cgagaagtac atcaggaagg ccgcgggtcc gtcgagcact tcgacagcga
     2101 agtaggttcg tggctatagt tgtcgcatgc gtctgttcgc gacatcagat ttacattccg
     2161 actacaagga aaactttaac tggctcaaac agctctcgga caccgcgtac cgcgacgacg
     2221 cgttgatcgt cgcgggcgat gtctctgatc ggttggagat cgttcgtgag actctgctcc
     2281 tgctgcgtgc gaagtttcgc cacgtcctct tcacgcccgg caatcacgag ctctgggtgc
     2341 ggggcgagcc gtttgattcc atcgaaaagc tgaagcgcat tttagacctc tgtgaagagc
     2401 tcgacgtaac tacacgtccg ctgcgtctgg acgatgtttg ggtggtgccg cttttctcct
     2461 ggtacgacgg cgtctttaat tccgaaaaag atgagcgtac cgcgcgactg tcgtgggttg
     2521 attttcacgt gtgcaaatgg cctgaagacg ccgctccgct tcccgattac tttctgcgga
     2581 tgaacgaacc gcatctcaag acttacgatg cgcctgtcgt aaccttctca cacttcatcc
     2641 cgcgttccga tatttttccg ccgaaggaat acttgagatt cagttggttg gaaagcgttt
     2701 cgctgtgtgc cggactggac catcagatca gaacgctaaa gtcgcataca cacgtctgcg
     2761 gtcatacaca cacgactttc gatctcgtca tcgacggcgt ccgatatatt caaaacgccg
     2821 tccgctaccc acgcgaacgc accgaaacaa gcgtccccat caagcagatc tagccacaag
     2881 aaggcacaaa aagcacaaaa tgaaattgac tggtttttaa cttttgtgtt ttttgtgcct
     2941 tcttgtggct agatctgctt atgcctcttt gtggccgttg cggcgcggga ttttttgtag
     3001 taagcgggcc agggtttgca ggtcgaaggg ttttgtgagg tgggcgtaga agccctgatc
     3061 ggaggcgcgc tgcacgtctt cggggcggcc aaatcccgtc aacgccaccg ccggcaaatc
     3121 ctgtttaccc ggcagatcac gcagcctgct caaaaactca aaaccatcca tctccggcat
     3181 cgaaatatct gacaacacca cgtcaaactc tcgctcacgc gcaaaacgca gcgcctcggc
     3241 accgctcgtc gccgcccaga ctgacgcgcc gccaatctcc aacaagtgac gcaccatctc
     3301 tgtcgtgtct tccgagtcgt cgaccaccag cacttccatt ccctggagtg tcccgatagt
     3361 tagatccatt gacaggctcg gccgacgttt cgtatcgacg ctgcgcggga gtcgaatcgt
     3421 aaacatcgca ccctgcccta ctccagcgga atatgccgcg accgagccgc cgtgcaactc
     3481 aaccaactgt tggaccacgg ccagaccaat gcccatgccc gactgcgcgc gattggtgcc
     3541 ggaatcagcc tggcgaaaga tctcgaagat atgtggcagg aacgacgtat caattccctg
     3601 cccgttgtcg gcgaccgtaa gcacaatgtc ctcgccgtct tcgtcgaggc gaacttcgat
     3661 gcgcccgcca cgcggcgtaa acttcaccga attgttcaat aggttccaga tgatctgctc
     3721 gagcctg
//
LOCUS       DASTEY010000476         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1345304, whole genome shotgun sequence.
ACCESSION   DASTEY010000476 DASTEY010000000
VERSION     DASTEY010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345304"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFO72_08355"
     CDS             <1..1174
                     /locus_tag="VFO72_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479341.1"
                     /translation="TSHPVVFDGKHSKLVIVNDVTERKLLDEKQQRLHTSLQQSAAEW
                     RQTFNAMDFPVLIVDLAGKIKRSNVAAEQIAGIAAEELAGRTIGELGNNQPWKKAAEF
                     IQNVRVNPAPVSEEIKDEVSGKTWAITLYHINEFGSFGERAILIAQDISKRAELEASL
                     RQSEMMSLLGSLVAGVAHEVRNPLFGISSILDAFETRFSNRTEYLRYTNVLRDEVGRL
                     TVLMEELLEYGKPFRGELFLVSMEELITRSIRACMPAAEVAHVTLNSEVEEALPKLRI
                     DRRRLSKVFVNLIENAIQHSPANGVVTVEARRLSDGGHEWVQCAVRDSGPGILAEDIP
                     KIFEPFFSKRRGGTGLGLAIAHRIMQEHGGKLIASNNPSGGACMIATFPTPREEAN"
     gene            1175..>2029
                     /locus_tag="VFO72_08360"
     CDS             1175..>2029
                     /locus_tag="VFO72_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9479342.1"
                     /translation="MSRNKVLVVDDESGVRFGIRDFLEQHGYEIDEAETCRDAQHIFR
                     TSRPDIVIADYMLPDGTALDLLPRLKEIDSSTPLLVLTAHGSIDLAVRAIKEGAEQFL
                     TKPLELPTLLVILQRLLQKQRNHHKQLASKSRQSRQAVDPFIGTSAAIRALKEQAHKI
                     LWTESPVLILGETGTGKGVLARWLHDNSPRAEEAFVDLNCAGLSRELLETELFGHEKG
                     AFTSATASKQGLFEVAHRGTIFLDEVGDVDLQIQPKLLKVLEEKRFRRVGDVRDRQVD
                     VRLIAATHQ"
BASE COUNT          496 a          525 c          592 g          416 t
ORIGIN      
        1 gacgtcacat ccggtggtct tcgacggtaa acacagcaaa ctcgtaatcg tcaatgacgt
       61 caccgaacga aaactgctcg atgagaaaca acagcggttg cacacgtcgc tccaacagtc
      121 ggcggctgag tggcggcaaa cctttaacgc gatggatttt ccggtgctga tcgtcgatct
      181 agcgggaaag atcaagcgct cgaatgtggc agcggaacag atcgcgggca tcgccgcaga
      241 agagttggct ggccggacga taggcgagct gggcaacaat caaccatgga aaaaagcggc
      301 cgagtttatc caaaacgtgc gggttaatcc ggcacctgtt tccgaggaaa taaaagacga
      361 agtcagtgga aagacgtggg ccatcacgct ctatcacatc aacgaatttg gttctttcgg
      421 cgaacgagcg attctcatcg cgcaggacat cagcaaacgc gccgaactcg aggcgtcgct
      481 gcggcaaagc gagatgatgt cgctgctggg atcgctggtg gcgggcgtgg cccacgaagt
      541 tcggaatccg ctgtttggca tttcgtcgat tctcgatgcg tttgagacaa gattcagcaa
      601 tcgcacggaa tacctgcgtt atacaaacgt tttacgcgac gaagtcggcc gcctgacagt
      661 tctgatggaa gagttgttgg aatacggaaa accgttccgt ggcgaactct ttctcgtgtc
      721 aatggaggaa ctgattacgc ggtccattcg cgcctgcatg ccggcggcgg aagtggccca
      781 cgtgacctta aacagcgagg ttgaagaagc tctgccgaag ctccgcatcg atcggcggag
      841 gttgtcgaag gtttttgtaa acttaatcga gaatgcgatt caacactcgc cggcgaatgg
      901 cgtcgttacg gttgaggcgc gacgccttag cgacggcggc cacgagtggg tgcaatgtgc
      961 tgtcagagat tccggtccgg gtattcttgc ggaagacatt cccaagatct tcgaaccatt
     1021 cttctcgaag cgaaggggtg gcaccggttt gggtttggct atcgctcacc gcatcatgca
     1081 ggaacatgga ggtaaattga tcgcgagcaa taatccaagc ggcggagcct gtatgatagc
     1141 cacgttccct acacccaggg aggaagccaa ctaaatgtcc aggaacaagg ttttggtcgt
     1201 cgatgatgaa tcgggtgtga gattcggcat tcgtgatttt ctcgaacagc acggatacga
     1261 aatcgatgag gctgaaacct gccgggacgc ccagcacatt tttcgcacgt ccagaccgga
     1321 cattgttatc gccgactaca tgttgccgga cggtactgcg ctcgacctat tgccgcggct
     1381 caaagagatc gactccagta ctccgctgct cgtgctcaca gcccacgggt caatcgacct
     1441 cgcggtgcga gcgattaagg aaggcgccga acagttcctg acaaagcctc tggaattgcc
     1501 gacgttactc gtaattctcc agcgtttact gcaaaaacag cgaaaccacc acaagcaact
     1561 ggccagcaaa tcacgacaat cgcgacaggc cgtcgatccg tttattggca cgagcgcagc
     1621 gatacgcgcg ttgaaagaac aagcgcataa aattttgtgg actgagagtc cggtgctcat
     1681 tctcggcgag acaggaactg gaaaaggcgt gcttgcgcgc tggttgcacg acaacagtcc
     1741 gcgcgccgaa gaagcattcg tcgatctcaa ttgcgccggt ctatcacgcg aactgctcga
     1801 aacagaactt ttcggtcacg aaaagggcgc gtttacgagt gccaccgcgt cgaagcaggg
     1861 actgtttgag gtggctcatc gcggcacgat ctttctggac gaggtgggcg acgtcgatct
     1921 gcaaatacaa ccgaagttat tgaaagtgct cgaagagaaa cgttttcggc gcgtcggcga
     1981 cgttcgcgac cggcaggtgg acgtgcggct cattgccgca acgcatcag
//
LOCUS       DASTEY010000477         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1328900, whole genome shotgun sequence.
ACCESSION   DASTEY010000477 DASTEY010000000
VERSION     DASTEY010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328900"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFO72_08365"
     CDS             <1..335
                     /locus_tag="VFO72_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HET9479343.1"
                     /translation="GEHKYFKRKGDNIYVTVPITVPEAALGAKIEVPTVDGKSQLRIP
                     PGTQSGQKFRLRDRGSPSLRNPGAKGQQFVEVQVTLPRVISEETKELLREYSKLNSTN
                     PRVEMGLE"
     gene            436..828
                     /locus_tag="VFO72_08370"
     CDS             436..828
                     /locus_tag="VFO72_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9479344.1"
                     /translation="MTRKPKTYTISAVADQYGIHPQTLRLYEREGLLKPSRSEGNTRL
                     YTDPDLERLELILSLTRDLGVNLAGVEIILNMREKMDAMQAEFERFFKYLQTHAEELT
                     NFEPEPEESDALVPLARVAALRRRSSHS"
     gene            1008..>2469
                     /locus_tag="VFO72_08375"
     CDS             1008..>2469
                     /locus_tag="VFO72_08375"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9479345.1"
                     /translation="MDQNGAVIPGAEVQATLPKTRLTRNTTSDAEGRYRLIQLEPGNY
                     VVRVSSSGFATQQIENVTAVSGQSLQFDVTLIPSTVTVETVVVTTAETPTVDTKRTVV
                     GSTLTSHETRSLPLATRSALDLVFTLPGVTEEPLSTRDLAEDRNSNPASTPEEAGLFS
                     LAGAPAYSNNLTIDGLDNNDDRAARERFQPSLEAIAEVQVITNQFSAEYGRASGVRVN
                     LRTRSGSRKFHGSGFYFFRDEALDANTFRNNSLGLARLPLQEHVSGFAFGGPLLPLRK
                     QKNTVFFVSYELSQTLDSAQIDTLVPVAQNNLYPLPRPTDLDQRRIEDVNSPALAAEV
                     APFIASISTPLRNTSVTARVDHQFSETHNGSFVYHGGRLVNLRQFGGGNRLADALQAR
                     RRNSDAISYSDSFVFSSALVNQLRFQYSRLAPSFEAQGGDKPVVLIALRDPLISGTLV
                     AGSSTLGGSDRKEDRAQFQHVLSLVRGNHSFKFGADV"
BASE COUNT          531 a          695 c          702 g          541 t
ORIGIN      
        1 ttggggagca caaatacttc aagcgcaagg gcgacaacat ttatgtgacg gtgccgatca
       61 ctgttccgga agcagcgctc ggggcgaaga tcgaagtgcc gactgtcgat ggaaagtcgc
      121 agttgcgaat tcctccgggc acgcagtcgg ggcagaaatt ccgcttgcgc gatcgtggct
      181 cgccgtcgtt gcgcaatccg ggcgcgaagg ggcaacagtt tgtggaagtg caagtgacgt
      241 tgccgagagt gatttcggaa gagacgaaag aactgctgcg agagtattca aagctaaact
      301 cgacgaatcc acgagtcgag atgggactgg agtgattgcc gattgccgat ttccaattgc
      361 cgatttgaaa caacagccgc gcccgcgagt gttcaaccgg caatcggaaa tcggaaatcg
      421 gaaatctaaa atgccatgac tcgcaaacca aaaacctaca cgatcagcgc ggtggcggat
      481 cagtatggca tccatccgca aacgctgcgg ctttacgagc gtgaggggtt gctgaagcct
      541 tcgcgctctg aaggcaacac gaggctgtac accgatccgg atcttgaacg actggaactg
      601 attctctctt tgactcgcga cctcggcgtc aatctggcgg gtgtggagat cattctcaac
      661 atgcgcgaga agatggacgc gatgcaggcg gagtttgaac ggtttttcaa gtacctgcaa
      721 acacacgccg aggagctgac gaatttcgag ccggagccgg aagagtcgga tgcgctggtg
      781 ccgcttgcac gtgtggcggc gctgcggcgt cgatcgtcgc acagttgatc ttgctcggcc
      841 gcaagtggta gattcctgcg cgttttcctc acatctcagc agcaggagtt tacaaaatga
      901 cctcacgtct cacgttcttt gtttgtctca atctcttcct tttagcttct aatcttcagt
      961 gcgctcactc gcaagatctg gataccgtca cgatctctgg tcgtgttatg gaccagaacg
     1021 gtgcggtgat tcccggcgcc gaagttcagg cgacgctgcc taagacacga ctgacgcgca
     1081 acactacaag cgatgccgaa ggccgttatc gtttgatcca actcgagccc ggcaactacg
     1141 tcgttcgcgt ttcctcttcg ggcttcgcga cacaacagat cgaaaacgtc acggctgttt
     1201 ccggtcagag tttgcagttt gatgtgacgt tgattccgtc aacggttacc gttgaaaccg
     1261 ttgttgtcac tacagccgaa acacctacag tagacaccaa acgcaccgtt gtcggttcga
     1321 cactcacctc gcacgaaacg agatcgctgc cactcgcgac gcgttccgcg ctcgatctgg
     1381 tcttcacctt gccgggcgtt acggaagagc cgctttccac gcgcgacctg gccgaggacc
     1441 gcaacagcaa tccagcgagc acgccggaag aagcgggtct tttctcgctg gccggcgctc
     1501 ccgcttattc aaacaacctg acaatcgacg ggcttgataa caacgacgac cgggccgccc
     1561 gcgagcgttt tcagccgtcg ctcgaagcga ttgcggaagt tcaggtgatc acgaaccaat
     1621 tctcggcgga gtatggccgt gcgtccggcg ttcgcgtgaa tctgcggacg cgcagcggtt
     1681 cgcggaagtt tcacggaagt gggttctact tcttccgcga cgaagctctc gacgcgaata
     1741 cgttccgcaa caactcgctt ggactcgcaa gattgccgct tcaggagcac gtcagcgggt
     1801 tcgcttttgg cgggccgctg ttgccgttac gtaaacagaa aaacacggtg ttttttgttt
     1861 cgtatgaatt gagccagaca ctcgacagcg cgcagatcga tacacttgtt cccgtcgcgc
     1921 aaaacaattt atatccgctg ccgcgaccga cggatctgga ccagcgccgc atcgaggacg
     1981 tcaattcgcc cgcgctcgcg gcggaagtgg cgccgttcat cgcatctata agcacaccgc
     2041 tcaggaacac gagcgtcacc gcgcgtgtgg accatcagtt tagcgagacg cacaacggct
     2101 cgttcgttta tcacggcggg cggctcgtca atctgcggca gttcggcggc ggcaatcgtt
     2161 tagccgacgc gttgcaggcg cgccggcgca actccgacgc aatctcatat tcggatagct
     2221 tcgtcttttc gtcagcgctc gttaatcaac tgcgtttcca atactcgcgt ctggcgcctt
     2281 cgttcgaagc gcaaggcggc gacaagccgg tggtattgat tgctttgagg gatccgctga
     2341 tctcaggcac tctcgttgct ggttcatcga ccttaggcgg cagcgaccga aaggaagacc
     2401 gagcgcagtt ccagcacgtt ctttctttgg tccgaggaaa tcattctttt aagttcggtg
     2461 cagacgttc
//
LOCUS       DASTEY010000478         5154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_669645, whole genome shotgun sequence.
ACCESSION   DASTEY010000478 DASTEY010000000
VERSION     DASTEY010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669645"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFO72_08380"
     CDS             <1..557
                     /locus_tag="VFO72_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479346.1"
                     /translation="DFDHAFEATFTRNRARRRQIAITDGEFTGVDLTNVDDASLISAY
                     KSRMQIFYLMVALISPESKAEEERFFPPPIKEIFERKPPAVSDEFPSFAAQLKRDAAT
                     MRAHLNQLAAQDRGVAERVTQFKVDLARPLEPPRHAVKPLTAYSKGRVLGVKDEYYQI
                     GDYAVIREGSQMRIIGIRFFSRLF"
     gene            complement(619..1224)
                     /gene="pdxH"
                     /locus_tag="VFO72_08385"
     CDS             complement(619..1224)
                     /gene="pdxH"
                     /locus_tag="VFO72_08385"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00558.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET9479347.1"
                     /translation="MEGLDETRIDSDPIRLFQGWLQDAVAAKLPLPEAMNLATVTPDG
                     KPTARMVLLKQVDDEGFVFYTNYLSAKARQLEINPYAALVFYWPQLERQVRVEGRVNK
                     TSAEESSEYFKTRPRESQIGAWASPQSDVIPAREVLEQRARELSEQYCDREIDCPAHW
                     GGFRLKPDRIEFWKGRVGRLHDRLLYELQPDGAWTIKRLAP"
     gene            complement(1224..1655)
                     /locus_tag="VFO72_08390"
     CDS             complement(1224..1655)
                     /locus_tag="VFO72_08390"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9479348.1"
                     /translation="MASHKKFTIEERVRWGDVDAARIIFYGAYVRFFEFAETELFREV
                     GLAYGQMFDELKVWLPRVHLECDFHRAARLDDLLEVSVYVGRFGTKSMRLDFEVRRKN
                     EAELIATAHFVLAAVKQNTFETGPIPAELRERLAPYHAHKQ"
     gene            complement(1710..2501)
                     /locus_tag="VFO72_08395"
     CDS             complement(1710..2501)
                     /locus_tag="VFO72_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9479349.1"
                     /translation="MSFTRVDDIQMAYTDTGLGLPVVLLHGYPFNRSLWSEQVNALSN
                     SYRVITPDLRGLGETDATQGPATMNQMAQDVAALMDQLEISRAAVGGLSMGGYVALAF
                     YKQFPSRVRALVLADTRAQADTEEGKQTRFQQAEKARAEGMAGIADSMLPKLLTPETV
                     SKRPEVVKRVRDMMLKTKPEGAAGALLGMAERDDQTPLLSQISCPTLIMVGQEDPITP
                     VQDSEKMHREIAGSRLVVIENAAHVSNLERTEQFNDELTRFLKQQ"
     gene            complement(2505..>5154)
                     /locus_tag="VFO72_08400"
     CDS             complement(2505..>5154)
                     /locus_tag="VFO72_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET9479350.1"
                     /translation="SRFQKNVQELQDRIRLKVETSIALLRAGTGLFAASDEVDAGEFD
                     RFVEQLDLEKNYPGIQGIGFSRYFTPAQKDAVVQKMRESGDPGFHVWPEGTREEYTAI
                     LYLYPHGNRNKTAIGYDMFAEPMRRQAMEQARDTGTPAASGRVRLVQEPEGPGLPAFL
                     IYMPVYQNGAPTNSVEERYKALVGYVYSPYRIDDFLGPITTAKKYDVSLQVYDGARID
                     DANFLHGAGSEPPPSDLRFTKTDTLDVAGRTWTMSYAIKPSFERGSSRPLLKYTIIIG
                     ALLSLLFFAVTRAEVRARTRAETWAEEVRESEEAITEANQRALLEYERLLERISSLSQ
                     VLGTARELNTIFRGLREFTKVSVPCDGFFVSLYDPVRNVRTACYGWGDGEEVDVSKLP
                     PMPVTESGPNSRAVRTGQVVITNDYMRATSANLSVIIGPDVDNGLRPETSLAVPMAVM
                     GRIIGTIEVQSYQPNAYRPEHVTAMSMAANLTAVAIENVRLLKLERTAREAAEESNRL
                     KDEFLATVSHELRTPLTAILGWARLLEDGALGRDMTQQAVDTIWRNAKAQAQIVDDIL
                     DVSRIITGNLYIDLRPTEVVPVVQNAINVVRPTADAKGIRIEAQLDNTPAKVSGDANR
                     LQQVIWNLLSNAVKFTNSGGRVQVKVTQANAAVEISVTDTGQGIRREFLPYVFDRFSQ
                     ADSTTTRHHGGLGLGLAIARHLVEIHGGTIRAQSRGEGQGATFTITLPLIDSAAKTPE
                     IAPATDKSLTRASQMLSGVNVLLVDDDSDTLKLMATALSRRQANVTAVSSAGEAIQAI
                     RQKRPDVLVSDIAMPDEDGYGLIEKVRSLENGETQSIPAVAITAYAKEEDRKRALSSG
                     FQIYLAKPIELTELVSVVARAARRDF"
BASE COUNT         1093 a         1584 c         1330 g         1147 t
ORIGIN      
        1 tcgatttcga tcacgccttt gaagcgacat tcacaaggaa cagagcgcgc cggcgtcaga
       61 tcgcgattac tgatggagag tttaccggtg tcgacctaac caacgtcgac gacgcaagtc
      121 tcataagcgc gtacaagagc cgcatgcaga tcttttactt gatggttgcg ctcatcagtc
      181 ccgagagcaa agcagaagag gagcggtttt ttccaccacc gatcaaggag atcttcgaac
      241 gaaaaccgcc cgcggtgtct gacgagttcc cttcttttgc ggctcaactc aagcgcgacg
      301 ccgcgaccat gcgagcacac ctgaaccaac tcgccgctca ggatcgcggg gttgccgaac
      361 gcgttaccca gtttaaggtt gatctggcgc ggccccttga accaccgcgg cacgcggtca
      421 aaccgttgac cgcatacagt aaaggcagag tgctcggcgt taaggatgag tactaccaaa
      481 tcggcgacta cgccgtcatc cgcgagggtt cgcaaatgag aatcatcggc atacgcttct
      541 tctcacgcct cttctgacgc atattcccgc atggactcag gggttcctca caatgcgcaa
      601 acgggcgagc aacgacgtct acggtgcgag tcgtttgatg gtccaggcgc cgtcgggttg
      661 caactcgtac agcagccggt cgtggagccg tccgacgcgt cctttccaaa actcaattcg
      721 atccggcttc aagcgaaaac cgccccaatg cgctggacaa tcgatttcac gatcgcagta
      781 ttgctcgctt aactctcttg cacgttgctc gagaacctct cgcgccggga tcacatcact
      841 ctgcggcgag gcccacgcgc cgatctggct ttcgcgaggc cgcgttttga aatactccga
      901 cgactcttcg gcggaagtct tgttgactct gccttcaacg cgcacctgac gttcaagttg
      961 cggccagtaa aagaccagcg cggcgtacgg attgatctcg agttgcctgg cttttgcgct
     1021 taggtagttg gtgtagaaaa caaaaccttc gtcgtccacc tgcttcaaca agaccatacg
     1081 cgcggtcggt ttgccgtcag gtgttacagt agcgagattc atcgcttcgg gcagcggcag
     1141 tttcgcagcg actgcatcct ggagccagcc ctgaaacagc ctgatcggat cgctgtcaat
     1201 ccgcgtctcg tctaagcctt ccatcattgt ttgtgtgcgt ggtaaggcgc gagccgctcg
     1261 cgcaactcgg ccggaattgg acccgtttca aatgtatttt gcttcacagc ggcgagcacg
     1321 aagtgcgccg tggcgatcag ctccgcttcg ttcttgcgac gtacttcgaa atccaatcgc
     1381 atcgacttcg tgccgaaccg tcccacgtaa acactgactt ccaacagatc atccagacgc
     1441 gccgcgcgat gaaagtcaca ttccagatgg acgcgcggca gccagacttt tagttcatca
     1501 aacatctgtc cgtaagccag accaacttcc cgaaacagct cagtctcagc aaactcgaaa
     1561 aaccgcacat aggcgccgta aaaaataatg cgcgcggcgt cgacgtcacc ccagcgcaca
     1621 cgctcttcaa tcgtgaattt cttgtggctc gccattaccg tgggaccttg tctatcttgg
     1681 cacttcaaag acagacaaga atgtctgtcc tactgctgct tcaaaaaccg cgtcaactcg
     1741 tcgttgaatt gctccgtgcg ttcgagattc gacacgtgcg cggcattctc gatcaccacc
     1801 aaacgcgagc ccgcaatctc gcgatgcatc ttttcactgt cctgcaccgg cgttatcgga
     1861 tcttcctggc ccaccataat cagagtcgga catgatatct gagacagcag cggcgtttga
     1921 tcgtcacgtt cagccattcc caatagcgcg ccggccgcgc cttcaggttt ggtcttcaac
     1981 atcatatcgc gcacgcgctt cacgacctcg ggtcgcttgc tgacagtttc aggcgtcaac
     2041 aacttcggca acattgaatc tgcaattccc gccatgccct cggcgcgtgc tttctccgct
     2101 tgttgaaagc gcgtctgttt tccttcttca gtgtctgcct gcgcacgcgt gtcggcgaga
     2161 accaaagcgc ggacgcgcga aggaaactgt ttgtaaaacg cgagcgcgac gtaaccaccc
     2221 atcgacagcc caccaaccgc ggctcgcgaa atttccagtt ggtccatcaa cgcagcgacg
     2281 tcctgcgcca tctggttcat cgtcgccgga ccttgcgtgg cgtcagtttc acccaacccg
     2341 cgaaggtccg gcgtgatcac gcgataactg ttactcagcg cattgacttg ttcgctccac
     2401 aatgaacgat tgaaaggata gccgtggagt agcacaacgg gcagaccgag ccccgtatca
     2461 gtgtaggcca tttgaatatc gtcaacccga gtgaacgaca tgatctaaaa atccctgcgt
     2521 gccgcgcgcg ctaccaccga aaccaactcc gtcaactcta tcggcttcgc taaataaatc
     2581 tgaaaaccgg acgacagcgc ccgcttgcga tcttcctcct tggcatacgc cgtgatcgca
     2641 accgccggaa tgctctgcgt ctcgccattt tccagtgacc gcaccttttc tatcaggccg
     2701 tagccatcct catcaggcat cgcgatgtca gacacgagca cgtccggccg tttctgcctg
     2761 atcgcttgaa tagcctctcc tgcggaagat accgcagtga cgttcgcttg tctcctgctc
     2821 aatgcagtgg ccatcaattt caaagtgtcg gaatcgtcgt cgaccagcaa tacgttcacg
     2881 cccgacagca tctgcgaagc gcgcgtaagg ctcttgtccg ttgccggagc gatctcggga
     2941 gtcttcgctg cggaatcgat cagcggcagc gtgatcgtaa acgtcgcgcc ctgaccttcg
     3001 ccgcggcttt gagcgcggat cgttccaccg tgtatctcga ccagatgacg cgcgatcgcc
     3061 aaacccagac cgagcccgcc atgatggcgc gtcgttgtgc tgtctgcctg actaaaacga
     3121 tcgaatacgt acggcagaaa ctccctacga atcccctggc ccgtgtccgt gacactgatc
     3181 tcaaccgcgg catttgcctg tgtgactttc acctgcactc gaccgccgct gttcgtaaat
     3241 ttcactgcat tcgacaacaa gttccagatg acttgctgga gacgattagc gtcgccggac
     3301 accttcgccg gcgtgttatc gagttgcgct tctatacgaa tgccttttgc atccgccgtg
     3361 ggccgcacca cattgattgc gttttggacc acgggaacca cttcggtggg acgcagatcg
     3421 atgtagaggt tgccggtgat aattcgcgac acgtccagta tgtcgtcaac gatctgtgcc
     3481 tgcgcctttg cgttgcgcca gatagtgtcg acggcctgct gcgtcatgtc acgaccgagc
     3541 gcaccatctt cgagcagacg cgcccaaccg agaatcgccg tcaaaggcgt gcgcaactcg
     3601 tgcgacactg tagcgagaaa ctcatccttg agacggtttg actcttcggc agcctcgcgt
     3661 gcagtgcgct ccagttttag taagcggaca ttttcgatcg caacggccgt caagttcgcc
     3721 gccatgctca tggcagtcac gtgttccggc cggtaggcat ttggttgata actctgcact
     3781 tcgatagtgc cgatgatgcg gcccatcaca gccatcggca ccgccagcga cgtttccggt
     3841 cgcaaaccgt tgtccacgtc cggcccaatg atcactgaga gattggcact tgtcgcgcgc
     3901 atgtaatcgt tggtgataac cacttgtccg gtccgcacgg cgcgactatt tggcccactc
     3961 tcagtcaccg gcatcggcgg cagtttcgaa acatcgacct cctcgccgtc gccccagcca
     4021 taacaggccg tgcgaacgtt acgcaccgga tcgtagagcg acacgaaaaa gccgtcgcag
     4081 ggaacagaga ctttagtgaa ctcacgcaag ccgcgaaata tcgtgttcaa ttcgcgcgcc
     4141 gttccgagca cttgcgagag actcgaaatt cgctcgagta gtcgttcgta ttccagcagg
     4201 gcgcgttgat ttgcttcggt gattgcctct tccgactctc taacttcttc cgcccacgtt
     4261 tccgcacgcg tgcgagcgcg cacttcagct cgcgttacgg caaagaacag cagactcagc
     4321 agtgcgccga tgatgatcgt gtacttcaac aacgggcggc tcgaaccgcg ctcgaacgaa
     4381 ggtttgatcg cgtagctcat cgtccaggtg cgtccagcaa catcgagcgt gtctgttttc
     4441 gtaaaccgaa gatcactcgg cggcggctcg ctaccggcgc cgtgcaggaa gttcgcgtcg
     4501 tctattcgag cgccgtcgta aacctgaaga ctgacgtcgt atttttttgc agtcgtgatc
     4561 ggaccaagaa aatcgtcgat tcgatacgga ctgtagacgt aacccacgag cgccttatac
     4621 cgctcttcga ctgaattggt cggagcgcca ttttggtaaa ccggcatata gattaagaaa
     4681 gccggcagtc ccgggccctc cggctcctgg accagtcgta cacgtcccga agctgcgggt
     4741 gtaccagtgt cgcgcgcttg ttccatcgcc tggcggcgca tcggctcggc aaacatgtcg
     4801 tagccaatcg ctgttttgtt ccggtttccg tgaggataga gatagaggat cgcggtgtat
     4861 tcctcgcgtg ttccctccgg ccagacatgg aagccgggat cacctgactc ccgcatcttc
     4921 tgcacaacgg cgtccttctg cgccggcgtg aagtatcgcg aaaaaccgat gccctgaatg
     4981 cccgggtagt ttttttccaa atcaagctgc tccacaaacc gatcaaattc gccggcgtcg
     5041 acctcatcgc tcgccgcaaa caaaccagtt cccgcccgca gcaaagcaat cgaagtctcg
     5101 acttttagcc ttattcgatc ctgaagttcc tgaacgttct tttgaaaacg gctc
//
LOCUS       DASTEY010000479         5101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1312534, whole genome shotgun sequence.
ACCESSION   DASTEY010000479 DASTEY010000000
VERSION     DASTEY010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312534"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFO72_08405"
     CDS             complement(<1..1082)
                     /locus_tag="VFO72_08405"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET9479351.1"
                     /translation="MATQKAFQEQLNPFEIAKQQFHRAADYLDLDPSLRHVLEHPKRQ
                     LIVSIPVKMDGGDVQVFEGYRVQHNIARGPAKGGIRYHPNVTLDEVKALASWMTWKCA
                     TVGIPYGGGKGGVICDTKSLSRNELERLTRRYAFEIAPIIGPDRDIPAPDVYTDEQTM
                     AWIMDTISMVRGHTELGVVTGKPISLGGSQGRSQATARGCLYALREACRVKGMSLKGS
                     RVVVHGFGNAGANIARLVAEDGAKVIAACDSKLAVYAESGIDMEEALKHKAETGSLSG
                     LRGTKETECDDILTMDCDILLPSALENAITLKNVGNVKAKIIAELANGPTTPGADRVL
                     EDAGVFLVPDILANAGGVTVSYYEWV"
     gene            1254..2048
                     /locus_tag="VFO72_08410"
     CDS             1254..2048
                     /locus_tag="VFO72_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9479352.1"
                     /translation="MFNNLIESSSHTKEYKRRGSFLLFTGATYVVLFVIAGVVSIYAY
                     DAHLERQSTDLELLTWVPQPPVEAVPEAVRDTVRPASNTATAPDRPTRTDLVDSTSNP
                     NNAPHDIGTVASSVPPAPRNAVLGPVNADPIAPPGGGRGTGGTGNTSVVIDMPDAPPP
                     PPPTPKPAPKVLKVSIVLNSEALYLPVPAYPILARQTRTQGTVIVQVLISEDGNVVSA
                     KATSGHPFLVPEAEKAAMRARFSPTMLNDQKVKVSGVITYNFKLDN"
     gene            complement(2119..2820)
                     /locus_tag="VFO72_08415"
     CDS             complement(2119..2820)
                     /locus_tag="VFO72_08415"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET9479353.1"
                     /translation="MPDETEELTVKVNDKESVTALLYPAAKKIRAGLTALLGHGAGAN
                     QLSGFMRMFARGLAARGLDVMTFNFVYMEQGRSVPDQKPKLESCFRAVIEAIAKHRKL
                     KNNRLVVGGKSMGGRIASQVVAAREDQPLALDVSGLVFLGYPLHPPGNPARLRVEHLE
                     HIQKPMLFVQGTRDALGTPEEIQPFVKDLRPPAKIYAIEGGDHSFKAPKKSGLPQEQV
                     YEAAMDEIVRWAVTI"
     gene            complement(2830..3726)
                     /locus_tag="VFO72_08420"
     CDS             complement(2830..3726)
                     /locus_tag="VFO72_08420"
                     /inference="COORDINATES: protein motif:HMM:NF045022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9479354.1"
                     /translation="MTYTVKPGDTLAKIAARNGLTLAQLLQANPQVSDPNRISVGDVL
                     NLPNGSASTETTRPLPTNPISSASTDNTRPLPGNPVAPAGVIGTTLAEALGALSAKYE
                     TGGRGPGTVSTGAGDPGGVSYGSYQMATKMGTVARFIGQTGFPWAQDFKGLTPGTAEF
                     TAVWKLIATNQTAEFQRAQHEFIKATHYDLLAAKVLAENDVDVNTRGRAVQDVIWSTA
                     VQHGGATPIVSRAIANLSCTSEDPAYDKQLICAIYAERGRKKPDGNLAYFSKSSPGVQ
                     AGVSKRFKNEEQDALAMLAKDV"
     gene            complement(3872..5035)
                     /gene="alr"
                     /locus_tag="VFO72_08425"
     CDS             complement(3872..5035)
                     /gene="alr"
                     /locus_tag="VFO72_08425"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9479355.1"
                     /translation="MSRLPDPQNNTPRRPTWAEIDLNQLAANFNQIKRRVGSTARVMA
                     VVKANAYGHGAVDCSRRLAAEGADWFAVALPEEAIELRDAGIKQPVLCLAGFWPGQAA
                     ACIQHKLTPVVYRRDMLEALNQAAANAGVIVDVHVKVDTGMGRLGVRYDQLSEFVGPF
                     EQFRNVRIDGVMTHLAAADDAACRPLTLDQIRRFDEAVSVFREHGYRPTHLHLSNSAG
                     VYGHREAWGNMVRPGGVLYGLWRDVLPLSVSQPQLGPVMSLYSRIVMLKWVPPGETIG
                     YGCTFEASRRSLIATLPIGYHDGYMRGLSNRAHVIVRGVYAPVVGRISMDLTLIDVTD
                     VAGVALHDEVTLLGWDRRNPELRIRAEDLARTAGTLSYEFTCGISDRVPRVYV"
BASE COUNT         1060 a         1543 c         1404 g         1094 t
ORIGIN      
        1 tgcacccact catagtaact caccgttacg ccacccgcat tcgccaggat atccggcaca
       61 agaaacactc ccgcatcctc cagcacgcga tcggctcccg gcgtggttgg tccattggcc
      121 agttcagcaa tgatcttcgc cttcacgttg cccacgttct tcaaagtgat cgcattttcc
      181 agcgccgacg gcaacagaat gtcacagtcc attgtgagga tgtcgtcgca ctcggtctcc
      241 ttcgtgcccc gaagccccga caacgaaccc gtttcagcct tatgtttcaa cgcttcctcc
      301 atgtcgatcc cgctctccgc gtaaaccgcc aacttcgagt cgcacgcggc gatcactttc
      361 gcgccgtctt ccgccacgag ccgcgcgatg ttcgcaccgg cgtttccgaa accatgtact
      421 acgacgcgcg agcctttcag gctcatcccc ttcacgcggc aggcttcgcg caacgcatag
      481 agacaaccac gcgccgtcgc ttgagagcgt ccctgcgagc caccgagcga gatcggtttg
      541 ccggtaacga cgccgagttc cgtatggcca cgcaccatgg agatcgtgtc catgatccac
      601 gccatcgtct gctcgtctgt gtaaacgtca ggcgcgggaa tatcgcggtc tggtccaatg
      661 atgggcgcga tctcaaaagc gtagcggcgc gtcagtcgtt cgagttcatt acgcgacaga
      721 ctcttcgtgt cgcaaataac gccacctttg ccgccgccgt aaggaatacc aacagtcgca
      781 cacttccagg tcatccatga ggccagtgct ttgacttcgt cgagcgtcac gttcgggtga
      841 taacgaatac cacccttggc tggaccacgc gcaatgttgt gctggacgcg ataaccctcg
      901 aacacttgca cgtcgccacc atccatcttt acggggatgg aaacaattaa ctgacgcttg
      961 ggatgctcca gcacatgccg cagcgacgga tcgaggtcaa ggtagtcggc ggcgcggtgg
     1021 aactgctgtt tggcaatctc gaacggattt agttgttcct gaaaggcctt ttgggtcgcc
     1081 atgcattccg ctcctggaaa gtgtgggatg tcgagagcgg cgtgatccta tgtggttacg
     1141 ggccagagtt caagttggct atcgtgctgg gaaccttctg gcggcgcgtg tgtctaatcc
     1201 atcgagtaag ctcccgcgac ccgaatggca ccggcaacca gcggaggaac tcgatgttta
     1261 ataacctgat tgaatccagt tcgcatacaa aggagtacaa gcgacgcgga tcgttcctgc
     1321 tctttaccgg tgccacttac gttgtgctgt tcgtcatcgc cggcgtggtg agtatctacg
     1381 cttacgatgc acatttggag cggcagtcta cggacttgga gctgcttacc tgggtaccgc
     1441 agccgccggt cgaagcagtt ccggaagcag ttagagatac ggttcggccc gcctcaaata
     1501 ctgctaccgc tccggatcgc ccgacacgca ccgacctggt cgacagcact tcgaatccca
     1561 acaacgcgcc gcacgatatc ggaaccgttg cgtcatccgt tccacctgcg ccacgtaacg
     1621 ctgtgctcgg accggtaaac gccgacccga tcgctccgcc tggaggcggt cgcggaaccg
     1681 gcggcacagg caacacgtca gtggtgatcg atatgcctga cgcaccgcca ccaccgccgc
     1741 caactccaaa accagccccg aaagttctga aagtctccat tgtgttaaat agcgaggccc
     1801 tgtatttgcc ggtgcccgcg tatccgatcc ttgccaggca aacaaggacg caaggtactg
     1861 tcatcgtcca agtgctgatc agtgaggacg gaaacgtagt ttcagcgaag gccacgtccg
     1921 gccatccgtt cctcgttccc gaggccgaga aggccgccat gcgagcgcgt ttctcgccaa
     1981 cgatgcttaa cgatcaaaag gtcaaggttt caggagtgat cacctacaac ttcaaactcg
     2041 ataattgaca gtcctgccaa agcaaagagg tacgaggcca tgcggctctc gtacctcttc
     2101 ccgccttgga ccaccaatct aaatcgtcac ggcccagcga acgatctcgt ccatcgccgc
     2161 ctcgtaaacc tgctcctgcg gcaagccaga cttcttcggc gctttgaacg aatggtcgcc
     2221 cccttcgatc gcgtagatct tcgccggtgg acgcagatct ttaacgaacg gttgaatctc
     2281 ctccggcgtg ccgagcgcgt cgcgcgttcc ctgcacaaac aacatcggct tttgaatgtg
     2341 ttccaaatgc tcgacgcgca accgcgcagg attgcccggt ggatgcagcg ggtaacccag
     2401 aaacaccaga ccactcacat ccaacgccag cggttgatct tcacgcgccg ccacaacctg
     2461 tgacgcgatt cgcccgccca tcgacttacc gcccacgacg agccggttgt tcttcagctt
     2521 cctgtgcttc gcgatcgctt caatcaccgc gcgaaaacag gattccaatt tcggcttctg
     2581 atccggaact gaacggccct gctccatgta cacgaagttg aacgtcatca catccagccc
     2641 gcgcgccgcc agaccacggg caaacatgcg catgaatccg ctcaactggt ttgcgccggc
     2701 gccatggccc aacaaagccg tcagcccggc gcgaatcttc tttgcggcgg gataaagcaa
     2761 cgcggtcacg gactctttgt cgttaacttt gactgtcagt tcttcggttt cgtctggcat
     2821 cgtgtttggt taaacgtcct tcgcgagcat ggccagtgca tcctgctctt cgtttttgaa
     2881 tcgtttagac acgccggctt gaacgcccgg cgaactcttc gaaaagtacg cgagattgcc
     2941 atcgggtttc ttccgcccgc gttcggcgta gatagcgcaa atcagttgtt tgtcgtaagc
     3001 cggatcttca gaagtgcaac tcaggttcgc gatggcgcga ctcacgatgg gcgtcgcgcc
     3061 gccgtgctgg actgcggtgg accagatcac gtcctgaacg gcacggcctc tggtgttcac
     3121 gtcaacgtcg ttctcagcga gtacttttgc agcgagcaga tcgtagtgag tcgctttgat
     3181 gaattcatgc tgtgctcgtt gaaactctgc tgtttgattg gtggcaatga gtttccagac
     3241 cgctgtgaat tctgcggtgc ctggcgtgag acccttgaaa tcctgggccc aggggaagcc
     3301 ggtttggcca ataaaacgag cgacagtgcc catttttgtt gccatttggt acgaaccgta
     3361 ggagacgcct cctggatctc cggcgcccgt cgagaccgtg cccggaccgc gaccaccggt
     3421 ttcatatttc gcggaaagag ctccgagcgc ttcggccaaa gtcgtgccaa tcacgccggc
     3481 cggcgcaacg ggattgccgg gcaggggccg cgtgttgtca gtcgaagctg acgatatggg
     3541 attagttgga agtggtctcg tggtttcggt tgaagctgaa ccgtttggca gattcaggac
     3601 gtcgccgaca ctaattctat ttggatctga gacttgggga ttggcttgta ataactgagc
     3661 cagggtcagg ccgttgcgcg cagcgatctt agctagcgta tcgcccggct ttactgtgta
     3721 agtcatgtct agaggaacct ccggttcgat ttggcgaggg ggagtcgaat agtagtcaac
     3781 cgttcgcagc agagtcaata aagagcgcct taaaccaggg accagattac acagattgtt
     3841 ttcataaaat ctgtgtaatc cgtggctact cctaaacgta aactcgtggc acgcgatcgc
     3901 tgatgccgca cgtaaactcg taggagagcg tgcctgcggt tcgcgcgaga tcttccgctc
     3961 ggatgcgcag ctcgggattg cgtcgatccc aaccgagcaa cgtgacctcg tcgtgcaacg
     4021 cgacgccggc aacgtcggtg acgtcaatca acgtgagatc catggagatg cgtccaacca
     4081 ccggggcata aacaccgcgc acgatcacat gagcacgatt tgatagtccg cgcatatagc
     4141 cgtcgtgata gccgatcggc aaagttgcaa taaggctgcg tctcgacgct tcaaacgtgc
     4201 agccgtagcc gatcgtttcg cccggtggca cccatttcaa catgacgatg cgcgagtaga
     4261 gcgacatgac tggaccaagt tgcggctgtg aaactgagag cggcagcaca tcgcgccaca
     4321 gtccgtacaa caccccgccg ggccggacca tatttcccca agcttcacga tgaccgtaga
     4381 cgccggcgga gtttgacagg tgcagatgcg tcgggcgata tccatgttct cgaaatacac
     4441 tcaccgcttc gtcgaaacgc cgaatctgat ccagagttaa ggggcggcag gcggcgtcat
     4501 ctgccgccgc gagatgcgtc atcacaccat cgatgcggac gttccgaaac tgctcgaacg
     4561 gtccgacgaa ttcgctcaac tgatcgtaac gcactccgag ccggcccatg cccgtgtcaa
     4621 cttttacgtg cacgtcgaca attactcccg cgttcgcagc ggcctggttg agcgcttcaa
     4681 gcatatcgcg ccggtaaaca acaggcgtca gtttgtgctg aatgcaggca gccgcctggc
     4741 ccggccagaa acctgcgaga caaagcaccg gttgcttgat tcccgcgtcg cgtaactcaa
     4801 tcgcttcttc gggaagcgcg acagcgaacc agtcagcgcc ttccgcggcg agccgccgtg
     4861 agcagtcgac ggcgccgtgt ccgtacgcat tggctttgac cacggccatc actcgcgcgg
     4921 ttgagccgac gcgccgtttg atctgattga aattggcggc cagttgattg agatcgatct
     4981 ccgcccacgt cgggcgacga ggtgtgttgt tttgtgggtc cggcaaacgg gacatgtctc
     5041 agtttaaccg atgctttgct ccgcaggcgc aggatgttta tggctttcgc ccggctgcac
     5101 g
//
LOCUS       DASTEY010000480         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_867488, whole genome shotgun sequence.
ACCESSION   DASTEY010000480 DASTEY010000000
VERSION     DASTEY010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867488"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFO72_08430"
     CDS             <1..998
                     /locus_tag="VFO72_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9479356.1"
                     /translation="GKTTAAEMAMDYTADNPVFGRTLHPQDPKLTPGGSSGGEAVAIA
                     TRMSPGGLGSDLAGSIRIPAHFCGICGLKPTTSRVPGEGQFPPSTGPYGLGAAIGPMA
                     RTVADLRLLFGVLSNQTQVVTDLRGSRFAWYVDDGVVPVTDETARAVANAAATLTDAG
                     LIGENRLPPHVQRGNELWLKLYSRASVVQLRQAYAGRESEGGSFVSWRLRTADDTPPP
                     TLDEYIANWMERDRLREELLRWMETTPIIVAPVGATPAYEHDTLKVTVQGSTMGTFRA
                     FSYAQAFNVFDLPVVTVPAGRSNDGLPIGVQIAGPAFAEEMVLAAAEIVEQVYNP"
     gene            1021..2031
                     /locus_tag="VFO72_08435"
     CDS             1021..2031
                     /locus_tag="VFO72_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9479357.1"
                     /translation="MELAASVLFLGFIAIVLLVIAVKTIRIVPQATVMLVERLGKFDK
                     VASSGLNILVPFLDKPRAVYWTNTRPGLTSIDLREQYIDLPPQPVITRDNVTIHVDSV
                     VYWQITDPVKAVYEMNDLVGGIVQLTITGMRAVMGDMDLDHTLSQRDQINSKLRLILD
                     EATDKWGVKVTRVDVKNINPPEDVRITMEKQMTAERNRRALVLQAEGDRQAAITRAEG
                     EKQAAVTRAEGEKQSAILAAEGAAQARLRAAEAESEAINRVANAIPGGDPGQYLITTR
                     YIDSLRDMTRTNNSKVIFMPIETSAMLSSIGAIKEVLSQTGESSDDRETPPPVRPREL
                     PR"
     gene            2089..2640
                     /gene="thpR"
                     /locus_tag="VFO72_08440"
     CDS             2089..2640
                     /gene="thpR"
                     /locus_tag="VFO72_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET9479358.1"
                     /translation="MFCAFELPEALRSRINQHAQRVREAVPEAAASWSRPENIHLTVK
                     FFGNVDRAKAPLISAAAARVVKEFSTIEIEVGKTGVFPRPSRPQVLWIGIGDPSGALL
                     KVQQNLEDEFAVDGFPKEDRAFRPHLTIARIRNPHNASRLAQTHLELEFSSVAVTLNE
                     LVLFRSELSSNGSKYTAISTHRL"
     gene            complement(2623..>3289)
                     /locus_tag="VFO72_08445"
     CDS             complement(2623..>3289)
                     /locus_tag="VFO72_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9479359.1"
                     /translation="DEKLRATLDAFEKWDGRINAESTVAPMLSQMRQTFRSKILAGAL
                     GPELVMRFQWNNFDTTLDRIIKDQPPEWLPKEFASYADLMRLSYDEAIATLTRNIGPD
                     EAKWTWGEMVKARFPHTLSPAPLIGAQFTVPPFPQSGTGGLLGATVNVGAAVSMRLIA
                     DLSNWDKTQQGIALGQSGLPKSPHWSDQLADWRAVTPREFPFTPAAVAKAAKQTLVLE
                     PVR"
BASE COUNT          719 a          911 c          985 g          674 t
ORIGIN      
        1 tcggcaaaac aaccgcggcg gaaatggcaa tggactacac cgcggacaat ccggtttttg
       61 ggcgcacgct gcatccgcag gatccgaagc tcacgcccgg tgggtcgagt ggtggggaag
      121 cggtcgcgat tgcgacgcgc atgtctcctg gtggattggg cagcgatctc gcgggatcga
      181 ttagaattcc ggctcacttc tgcggcatct gtggtctgaa accgacaacg agtagagtgc
      241 ctggcgaggg acagtttccg ccgagcactg gtccgtacgg cctgggagcc gcgattggac
      301 caatggcgcg aacggttgcg gatctgcgat tgctgtttgg agttttgagt aatcagacac
      361 aggtggtaac ggatttaaga ggatcacgat tcgcgtggta cgttgacgat ggagttgtgc
      421 cggttacgga tgaaactgca cgagcggttg caaatgcagc agcgacatta actgatgccg
      481 gattgattgg tgaaaatcgc cttccaccgc acgttcaacg cggcaacgaa ctatggctca
      541 agctatattc gcgcgcctct gtggtccaac tgcgccaggc ttatgctggt cgagaaagtg
      601 aaggcggttc ttttgttagt tggcggttgc gtactgctga cgacacgcct ccgccgacgt
      661 tggatgaata catcgcgaat tggatggaac gtgatcgctt gcgtgaagag ttgctgcgct
      721 ggatggaaac cacgccgatc atcgtcgcgc cggtcggagc gacacccgcc tacgagcacg
      781 acacactgaa agttacagtg caaggctcga ctatggggac gtttcgcgcc ttcagttacg
      841 cgcaggcttt caacgttttc gatctgccgg tggtgacagt ccccgcaggt cgttcgaacg
      901 acggcctgcc gattggcgtt cagatagcag gacctgcgtt tgcggaagag atggttttag
      961 cggcggcgga gatcgtcgag caggtatata atccgtgaaa cctgtggaaa ggaaaatctt
     1021 atggaactag ctgcctcggt tctgttcttg gggtttatcg caatcgtgtt actcgtcata
     1081 gcggtcaaaa cgatcagaat cgtgccgcaa gcgaccgtta tgctggtcga gcgtctaggc
     1141 aaattcgata aggttgcgag cagcgggctc aacattctcg ttccctttct cgataagcca
     1201 cgcgccgttt actggaccaa cacgcgaccc ggcctgacgt caatcgactt gcgcgaacag
     1261 tacatcgatc tgccgcccca accggtcatt acgcgcgaca acgtcacaat ccatgtcgac
     1321 tccgtcgttt actggcagat caccgatcca gtgaaagctg tttacgagat gaacgatctg
     1381 gtcggcggta tcgtgcaact cacgatcacc ggcatgcgcg ccgtcatggg cgacatggac
     1441 ctggaccaca cactctcgca acgcgaccag atcaattcaa aactccgtct cattctcgat
     1501 gaagcaactg acaagtgggg cgtgaaggtg actcgtgtgg acgtgaagaa catcaaccca
     1561 cccgaagacg tgcgcatcac catggagaaa cagatgacgg ccgagcgcaa tcgacgcgcg
     1621 ctggtgttgc aagccgaggg cgatcgacaa gcggcgatca ctcgcgctga aggtgagaag
     1681 caggccgccg ttacacgtgc tgaaggtgaa aagcagtcgg cgatcctcgc tgctgagggc
     1741 gcggcccagg cgcgcttgcg tgcggctgaa gccgaatcgg aagcgatcaa ccgcgtagca
     1801 aacgcgattc ccggcggcga tccgggacag tacttgatca cgacgcgtta cattgacagc
     1861 ttgcgtgaca tgacgcgcac caacaattca aaagtgatct tcatgccgat cgaaacgtcg
     1921 gcaatgctct cgagcatcgg ggccatcaag gaagtgcttt cacaaaccgg cgaatcgagt
     1981 gacgacagag agactccacc gccggtacgt ccgcgcgagt tgccacggta aagacggcca
     2041 cggatgacca cgggcgatac ggatcaagag cgaagaacgc ggtggcggat gttttgcgcg
     2101 ttcgagcttc ccgaggcgtt gcgttcacga atcaatcagc acgctcagcg agtgcgtgaa
     2161 gctgttcccg aagcagcggc gagttggagc cgtcctgaga acattcattt gaccgtcaag
     2221 ttctttggca acgtagatcg ggcaaaggcg cctttgatct ccgcggcggc ggcgcgtgtc
     2281 gtgaaagagt tttccacgat tgagattgaa gtggggaaga cgggagtttt cccgcggccg
     2341 agtcgcccgc aagtgttgtg gatcggcatt ggcgatccgt ccggcgcgct gttgaaggtt
     2401 cagcaaaacc tggaagacga atttgcggtt gacggctttc caaaagaaga tcgcgcgttt
     2461 cgaccacacc tgacaatcgc gcgcattcgc aacccacaca acgccagccg cctggcgcaa
     2521 actcacctcg aactcgaatt cagcagcgtt gcggtgacgc tgaatgaact cgtgctattc
     2581 cgcagcgagt tgagcagcaa cggctcgaag tacaccgcga tctcaacgca ccggctctag
     2641 cactagcgtc tgcttcgcag cttttgccac cgccgcggga gtgaacggaa actcgcgcgg
     2701 ggtgacggcg cgccagtcag caagttgatc gctccagtgt ggactcttcg gcaggccgga
     2761 ttgaccgagc gcgataccct gctgtgtctt gtcccagttg ctcagatctg caatcagtcg
     2821 catcgagact gccgcgccaa cgttgactgt tgcgccgagc aggccgccgg ttccgctttg
     2881 cggaaacggt ggcacagtga actgcgcgcc aatcaacggc gcggggctca gcgtgtgtgg
     2941 gaaacgcgcc ttcaccatct cgccccaggt ccatttcgct tcgtccggac cgatgtttct
     3001 cgtgagcgtt gcaatcgctt cgtcgtacga caaacgcatc aggtcagcgt agctcgcaaa
     3061 ttctttcggc aaccactcgg gcggctgatc tttgatgatg cggtcaagcg tagtgtcgaa
     3121 gttgttccac tgaaaacgca tcaccaactc cggaccaagt gcgcctgcga gtatctttga
     3181 gcgaaacgtt tggcgcatct gcgaaagcat cggcgcgaca gtcgattcgg cgttgattcg
     3241 tccatcccac ttctcgaacg catcaagcgt cgcgcgcagt ttctcgtca
//
LOCUS       DASTEY010000481         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_406037, whole genome shotgun sequence.
ACCESSION   DASTEY010000481 DASTEY010000000
VERSION     DASTEY010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406037"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /gene="dnaB"
                     /locus_tag="VFO72_08450"
     CDS             complement(<1..1317)
                     /gene="dnaB"
                     /locus_tag="VFO72_08450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9479360.1"
                     /translation="MAAPDIVRDHAFERALPNSSEAERAILGAVLLDNGLISQAIEHL
                     RPEDFYVPSHRRIFVAMIALFERGAEINAILIGEELKKENALESVGGISFITNLTYGL
                     PHSTNILHYAKVVRGKSMLRQLIKSANKITQEALAEEDEPEIILDHAEQAIFQLADER
                     IRQGFMHVKPIAEQLLEKVQEMEGRAAVLTGLTTGFPDLDKMTSGLQNSDLIILAARP
                     SMGKTSFALMLAENAAIHAGAVVGMFSLEMSKESLVMRMLCSQGNIDAQRFRNGFLSR
                     LEWAQIAKSLGILADTKIFLDDTPGLSVLEMRAKARRLAAEQKKLDLIIVDYLQLMSG
                     SARRVESRQQEVSQISRELKGLAKELNVPLVALSQLSRAPESRSDHRPQLADLRESGA
                     LEQDADLVAFIFREEQYKSPEERDAMPEDRKNIAELIIAKQRNGPTG"
     gene            complement(1412..1942)
                     /gene="rplI"
                     /locus_tag="VFO72_08455"
     CDS             complement(1412..1942)
                     /gene="rplI"
                     /locus_tag="VFO72_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9479361.1"
                     /translation="MAHTKVLLREDVDDLGARGEIVRVRAGYARNYLLPRNLAVEASA
                     GNVKGIEQERAALLRKEAKERATAEAQSQQMSSLVLEFKRKAGEQGALYGSVTSMDVA
                     EALQERGYEIDRHRIHLREPLKRVGDYTVPVRLHREVTIELQVKVVPEGEVIVGHLTP
                     DEEAELNKEPEASDAE"
BASE COUNT          403 a          653 c          535 g          451 t
ORIGIN      
        1 gccggttggg ccattgcgct gcttcgcgat gatcagttcg gcgatgttct tgcgatcctc
       61 gggcatcgcg tcgcgttctt caggcgactt gtactgctcc tcgcgaaaga taaatgccac
      121 caggtcggcg tcctgctcca acgcgcccga ctcccgcaag tccgccaact gcggccgatg
      181 atccgaccgc gactccggcg cgcgtgaaag ctgcgacaac gcaaccagcg gcacgttcaa
      241 ctccttcgcc aaccctttca actcgcgcga gatctgcgac acttcctgct gccgcgactc
      301 aacacgtctt gccgaacccg acatcaactg caagtaatcc acgatgatca ggtcgagttt
      361 cttctgctcc gccgcgaggc gacgcgcctt cgcacgcatc tccaacacac tcaaacccgg
      421 cgtgtcgtct aaaaagatct tcgtatccgc gagaatcccg agtgacttcg caatctgcgc
      481 ccactcaagc cgcgaaagga atccgttgcg aaaacgctgt gcgtcgatgt tgccctgcga
      541 acagagcata cgcatcacca gcgactcctt cgacatttcc agcgaaaaca taccgacaac
      601 cgcgcccgcg tgaatcgccg cattctccgc cagcatcaac gcgaaactgg tctttcccat
      661 cgacggccgc gccgcgagaa tgatcaagtc ggagttctga agtccggaag tcatcttgtc
      721 gagatccgga aaaccggtgg tcagtccggt gagcaccgcc gcccggcctt ccatctcctg
      781 aaccttttcc agcagttgct cggcgatcgg cttgacgtgc atgaacccct ggcgaatgcg
      841 ttcgtcggcc agttgaaaga tcgcctgctc cgcgtggtcg agaataatct cgggctcgtc
      901 ttcttcggcg agtgcttcct gcgtgatctt gtttgcgctc ttgatgagtt gccgcagcat
      961 cgacttcccg cgcacgacct tcgcgtaatg caggatgttc gtcgaatgcg gcagaccgta
     1021 ggtgagattc gtgatgaaac tgatcccgcc gaccgattcg agcgcgttct cttttttgag
     1081 ctcttcgccg atcaggatcg cgttgatctc ggcgccgcgc tcaaacagtg cgatcatcgc
     1141 cacgaaaata cggcgatgcg acggtacgta gaagtcttcg ggacgaagat gctcgatcgc
     1201 ctggctgatt aaaccgttat cgagcagaac ggcgcctaaa attgcgcgct ccgcctcgga
     1261 actgtttggc agagcgcgtt cgaatgcgtg gtcacgaacg atgtcaggag cggccataca
     1321 agaagtttgc aggagaaatt ttcgttgcgc aaaaatagac gccgcacgtc tttaacatgc
     1381 ggcgtcactc agcgcaatca atccaccggt tttattctgc gtcgctcgct tctggttcct
     1441 tattaagctc cgcttcctcg tcaggcgtga gatgcccaac gatcacttcg ccttccggca
     1501 cgactttcac ctgaagctcg atcgtgacct cacgatgcag tcgcacgggg acagtgtaat
     1561 cgcccacgcg cttcaacggc tcgcgcaggt gaatccggtg acgatcgatc tcgtaaccac
     1621 gttcttgcaa cgcctcggca acgtccatcg acgtgaccga accatacaac gctccctgct
     1681 cgcccgcttt acgtttgaac tcgagcacca gcgaactcat ctgctgcgac tgcgcttcag
     1741 ccgtagcccg ctccttcgct tctttgcgca acagcgcggc gcgttcctgt tcgattccct
     1801 tcacgttgcc cgcactggct tcgacagcga gattgcgcgg cagcaggtaa ttgcgggcgt
     1861 aaccagccct gactctgacg atctcgccgc gcgcgccgag atcatccaca tcttcgcgta
     1921 aaagaacttt cgtgtgtgcc atgttacttc tcctatcgct tagtcggtgg tgtacggcag
     1981 cagcgcgatg ttgcgggcgc gcttgattgc ttccgccaac atgcgctggt gagtcgcaca
     2041 gt
//
LOCUS       DASTEY010000482         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1312588, whole genome shotgun sequence.
ACCESSION   DASTEY010000482 DASTEY010000000
VERSION     DASTEY010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312588"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFO72_08460"
     CDS             complement(<1..466)
                     /locus_tag="VFO72_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479362.1"
                     /translation="MTRATQRLIAFAAHLLLLAFVCVPAVNAQDAQPQATPQATPLPA
                     PPPFKMIVKEERDALEQQSRDASKRLKLTIAYAGGHLTRAEQHTARENFEAASAEIGM
                     YHALVESALAFLSTFKRDSNKTRDLYKRLELSLRADGPRLTAMRRVTPLEYAV"
     gene            complement(468..1829)
                     /locus_tag="VFO72_08465"
     CDS             complement(468..1829)
                     /locus_tag="VFO72_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479363.1"
                     /translation="MEAFGDDAADPVRLFERGQSAHSRGELEKALAFYEQALKVRPEF
                     PEAEFQRGNALASLNRLPEADAAFRLAISHKKNWALPYSALGALLMRQDRDKEAEEMF
                     RQALAVDAKEGVALRMLSEIRLRAGDTKSALEFAKRATAIPEAPASAWVGLAVAEKAN
                     GNKVAAKAALDRVLGDEPGNFAALVERADLFTDEKNFELAIADLKAAAKLKPADKAVA
                     SRLANVLQQAGKADEALAVAREAGLEVQQPTGNGTGGVVGTPEEIEAANSTDPAVARK
                     ALEKLLEKNPRSAMLLSNLGASYRTDDPARSLDFYRRAAEIQPDSAEYALGYASALVQ
                     ARRFPEAAHILRQVVKATPDNYVAHANLATALYEAKRYQEAIPEYEWVLAVKPEIAVA
                     HYFIATSHDYLGEYVEALASYEKFLSAADAKTNQLEIDKVKLRLPTLRRQIKLGEGVK
                     KKP"
     gene            2044..>2847
                     /gene="larE"
                     /locus_tag="VFO72_08470"
     CDS             2044..>2847
                     /gene="larE"
                     /locus_tag="VFO72_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET9479364.1"
                     /translation="MNNPIAKEERLREIFRELESVVVAYSGGVDSSYVAYIANSELGP
                     RAVCITGQSASLPAYQRAEIDRVVEKFGFQHEVIQTEELDNPGYSANNPDRCFFCKDE
                     LYTRLESVARGRGIENIVDGSTVDDLGDYRPGRRAALQHAVRSPLIEVGLNKNEVREL
                     SRRATLPTWDKPASPCLSSRIAYGTTVTIERLSKIDRGEEILREFGFREFRVRHHDQL
                     VRLEIAQAEMDRIMSKEVFQNLAARFRELGFKYVTLDLEGFRSGSMNEVL"
BASE COUNT          573 a          788 c          834 g          652 t
ORIGIN      
        1 atactgcata ttcgagcggc gtaacgcgtc gcatcgccgt caagcgtgga ccatcagcac
       61 gaagcgacaa ctcgaggcgt ttataaaggt cgcgcgtttt gttgctgtcg cgtttgaagg
      121 tactaagaaa agccagagcg ctctcgacca gagcgtgata catgccgatt tccgccgacg
      181 ctgcctcaaa attttcgcgc gcggtgtgtt gctcggcacg cgtcaggtgg ccgcctgcat
      241 atgcgattgt gagcttcagt cgcttactcg catctcttga ctgctgctcg agcgcatcac
      301 gttcctcttt gacgatcatc ttgaagggag gcggcgccgg aagcggggtt gcttgtggcg
      361 ttgcttgtgg ttgcgcatcc tgagcattga ccgcgggaac acacacgaac gcgagtagta
      421 acagatgtgc cgcgaaagcg atcaagcgtt gtgtggcgcg agtcatgtca gggctttttc
      481 ttgacgccct ctcctaactt gatttgccgc cggagtgtcg gcagtcgtag ttttactttg
      541 tcgatctcga gctggtttgt ctttgcgtct gcggcagaca agaacttctc atacgatgcg
      601 agtgcttcga cgtattctcc aagataatcg tgtgaagtgg caatgaaata atgtgccacc
      661 gcgatctcag gcttaacagc taagacccat tcatattccg gtatcgcttc ctggtagcgt
      721 ttcgcttcgt acaacgcggt cgcgagattc gcatgcgcga cgtagttgtc gggagtggcc
      781 ttcacaacct gtcgcaggat gtgtgctgct tccggaaaac gacgagcctg gaccaatgcc
      841 gaggcgtatc cgagcgcgta ctccgcggag tctggttgga tttccgcggc acggcgatag
      901 aagtcgagtg aacgtgccgg atcgtcagtg cgatacgacg caccgagatt gctgagcagc
      961 atggcgctgc gcggattctt ctcgagcagt ttttcgagcg ccttgcgagc gacggcgggg
     1021 tcagtgctgt tggccgcttc gatctcttcc ggtgttccga ctactccgcc tgtgccgttg
     1081 ccggttggtt gttgaacctc gagacctgct tccctggcca ctgccagcgc ttcatccgct
     1141 ttacctgcct gttgcagaac gttggcgagg cgcgacgcca ccgctttgtc ggccggcttc
     1201 aacttcgccg ctgctttgag atcggcgata gcgagctcga agtttttctc gtcagtaaag
     1261 agatcggcgc gctccacgag tgccgcgaag ttcccaggct catcgccaag tacgcgatcg
     1321 agtgccgctt tggcggcgac cttgttgccg ttcgctttct cggcgacggc gagaccgacc
     1381 cacgccgaag caggagcttc ggggatcgca gtagcacgtt ttgcaaattc gagcgccgac
     1441 ttcgtatcac ccgcacgcag gcggatctcg gagagcatcc gcagcgccac gccttcttta
     1501 gcgtcgactg ccagtgcttg ccggaacatt tcctcggctt ctttgtcacg atcctgtcgc
     1561 attaacaaag caccgagtgc ggaataaggc agcgcccagt ttttcttgtg ggaaatcgcg
     1621 agacgaaacg cggcgtctgc ttcgggcaag cgattcagag acgcgagcgc gttgccgcgc
     1681 tggaactctg cttcgggaaa ctccggcctc actttgagcg cttgttcgta gaatgccagc
     1741 gctttttcga gctcgccacg cgagtgcgcg ctctgtccgc gttcgaacaa acgcaccgga
     1801 tctgccgcat cgtcaccaaa cgcttccacc gtttgcgcgg aaacagttgc acacagggag
     1861 aacagcgcga aacaactgac aataatcgac ggcagcgaca gtcttttccg gaaagaggtg
     1921 ctcaattcat gcattttgaa gcgtgatccg gggaggcttc gatgttagca caacaccacg
     1981 cagatcgtcg ctgtgatgct ggttgcgcag ctattgatgc atgagctaac atgcgggcac
     2041 cgaatgaata atcccatcgc caaggaagaa agactgagag aaatctttcg tgagctcgag
     2101 tcggtagtcg ttgcttactc gggcggcgtc gacagcagtt acgttgctta catcgccaac
     2161 tccgaattag gaccacgcgc ggtctgcatc acaggacagt ccgcaagcct gcctgcttac
     2221 caacgcgcgg agatcgatcg cgtcgtcgag aagtttggtt ttcaacacga agtgattcag
     2281 acggaggagt tggacaatcc cggttatagc gctaacaatc cagatcgctg cttcttctgc
     2341 aaggatgagc tgtacaccag gctcgagtcg gttgcgcgcg ggcgcggcat cgagaacatc
     2401 gttgatggtt cgactgtcga cgatctcggt gattaccggc ctggccgacg agcggcgctg
     2461 cagcatgccg tgcgtagtcc tttaattgaa gtcggtctaa ataagaatga agttcgcgag
     2521 ttgagccgcc gggcgacgtt gccgacgtgg gacaagcccg caagcccttg tctttcctcg
     2581 cgcattgcct acggaacgac tgtgacaatc gagcgactta gcaaaatcga tcgcggtgaa
     2641 gagatcctgc gcgagtttgg ctttcgagag ttccgggtgc gccatcacga tcagctcgtg
     2701 cgactcgaga ttgcgcaggc ggagatggac cgcatcatga gcaaagaggt gtttcaaaac
     2761 cttgcggccc gctttcgtga gttgggattc aagtacgtca ccctcgacct cgaaggcttc
     2821 cgcagcggct caatgaacga agttcta
//
LOCUS       DASTEY010000483         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_669703, whole genome shotgun sequence.
ACCESSION   DASTEY010000483 DASTEY010000000
VERSION     DASTEY010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669703"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFO72_08475"
     CDS             <1..608
                     /locus_tag="VFO72_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9479365.1"
                     /translation="VVNPGGPGSTESAGGVPGNSLLVIERLDPIYADFTISQNNLTAV
                     QQQMRAGTLSAEVRLPDSADDPISGPLTFIDNAVQSETGQVTLRATLPNPGHRFWPGR
                     FVNVRLLLGRVQGAVLVPASAPQMSAAGSYVYVIKPDSTAEQRTVSLGQRQGDLIVVE
                     QGVAAGEKVVVNGQLGVIPGGKVVIEQPQNPASVATDGSKS"
     gene            605..>3445
                     /locus_tag="VFO72_08480"
     CDS             605..>3445
                     /locus_tag="VFO72_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9479366.1"
                     /translation="MNLSEPFIRRPVMTAVLTLSVILFGVLSYFQLPVNDLPAVDYPV
                     IQVNVGYPGASPDTVANNIATPLERQFMQINGLEVVTSRSTQGFASFTLQFALDKSID
                     AAATDVQTAISQAISSLPTDLPSPPTYSKTNPNDQPIMYLAMVSDSVTTGQLYDFAST
                     QVGQRLSILPGVSQVNVYGTKSAIRIKANPKELWARGISIDDLNNAIRNGTSYTGAGQ
                     LDSPSKTAILRPQGQLETAEQYANLIVGGTNNAPVYLRDVATVKDSVQDERVNMRFWV
                     RGREVPAATVVIAVNRRAGANAIEVSKSIHETLPAVRAELPGSIDIMPVYDRAASIVH
                     SVADVQATLIIAFVLVVIVIFAFLGRVTDTLIPVVALPLSLLITFMAMRGLGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRIERGEPPFEASINSAKEISFTIVSMTISLAAV
                     FIPLVFMTGLVGRIFREFAITIVIAILASGLVSLTLTPLMCARLLKERGHGLKKTWVE
                     RVIGGIEKRVLGLYGGSLWWFLRHRWVSLVIWVVCLVGTVWLFMLVPKSFLPPGDSGV
                     IFGAFIAQEGSSPKQMREYQDRVDRMLHEDPNVITDFTLTGATGFLSSNTGITFTYIG
                     PPENRKPIEDVTAEMMGKLNTIPGIMTFLRPWPVREISTGVTNQTQGQYAFTVSGVNP
                     SQVYETGQKLLEKLQPSPLFLSVSSDLYNNTPNVNIDIRREQAKTYGVSEARILTLLR
                     NAYSQNYLYLIKQPEDQYQVILEMDDSVREKPEDLSLLYIKSDDGARLIPLSELVTWK
                     STLGPQSVNHFNQFTSMTLSFNLKPGVALGDATDFITKSAQEVVPQGVRAELQGEALT
                     FSRTVTSLTILMGLAVFVMYVILAILYESYVHPLTVLSTLPTALVGGLLTLIVFGAEA
                     SLYAFVGMFMLMGIVKKNGIMIVDFARHRVEAG"
BASE COUNT          731 a          945 c          969 g          800 t
ORIGIN      
        1 acgtcgtgaa tcccggcggt ccaggcagta cggaatccgc cggtggcgtg cccggcaatt
       61 cgctgctggt gatcgagcgg ctcgatccca tttacgccga ctttacgatc tcgcagaaca
      121 atttgaccgc ggtgcagcaa cagatgcggg ccggcacgct cagcgccgag gttcgacttc
      181 cggactccgc agacgatcca atctcgggac cacttacgtt catcgacaac gccgtgcaaa
      241 gcgaaacagg acaggtcacc ctgcgcgcga cattgccaaa tcccggtcac agattctggc
      301 ccgggcgttt cgtcaatgtg cgactgctac tcggaagagt tcagggcgcg gtgctcgttc
      361 ctgcctcggc gccacagatg tccgcagccg gctcatacgt ttacgttatc aagcctgatt
      421 ccactgccga gcagcgcacc gtctcgctgg gacaacgaca aggcgatctg atcgtcgttg
      481 aacaaggcgt tgcggctgga gaaaaggtcg tcgttaatgg acagctcggc gtgatccctg
      541 gcggcaaagt agttatcgag caaccacaga atccggcctc agtggctaca gacggatcga
      601 agtcatgaat ctctctgaac cattcatccg gcggccagta atgaccgcgg tgttgactct
      661 ttcagtgatc cttttcggag tgctgagtta ctttcagttg ccggtaaacg atctgcccgc
      721 cgtcgactat cccgtcatcc aggtaaatgt cggttacccg ggtgcgagtc cggatacggt
      781 cgcaaacaac atcgccacgc ccctcgagcg ccagttcatg cagatcaacg gcctcgaggt
      841 tgtgacttca cgaagcaccc agggcttcgc aagctttacg cttcagtttg ctctcgacaa
      901 gagcatcgac gccgcggcaa ccgacgtgca aactgcaata tcgcaagcga tcagcagcct
      961 gcccacagat cttccgtcgc caccaacgta ttcaaaaact aatcccaacg atcagccgat
     1021 catgtatctc gcaatggtca gcgactcggt gacgacgggt cagttgtacg actttgcgag
     1081 tacgcaggtg ggccagcgct tgagcattct gcccggcgtg tctcaggtaa acgtttacgg
     1141 aaccaaatcg gcgattcgca tcaaggctaa tccaaaggaa ttgtgggcgc gcggaatttc
     1201 gatcgacgat ctcaataacg ctatcaggaa tggaaccagt tacacgggcg cgggccagtt
     1261 ggatagtcct tccaaaaccg cgatcctgcg accgcaaggc cagctcgaaa ctgccgaaca
     1321 gtacgcgaac ttgatcgtcg gcggcacgaa caacgcgccg gtgtaccttc gggacgtagc
     1381 tacggtcaag gactcggtcc aggacgaacg cgtaaacatg cgcttttggg tgcgtggccg
     1441 tgaggtaccc gcggcgactg tagtcatagc ggtgaatcgc cgcgccggcg caaacgcaat
     1501 tgaagtgtcg aaatcgatcc atgaaactct gccggctgtc cgtgcagaac tgcctggttc
     1561 gatcgatatc atgccggtct acgatcgtgc cgcaagcatc gtccactcag tcgctgacgt
     1621 gcaggcaacc ctcatcatcg ctttcgtgct ggtcgtgatc gtcatctttg cgtttcttgg
     1681 ccgggtgact gacacgctga tacctgtagt cgcactgcca ttgtcgttgc tgatcacgtt
     1741 catggcgatg cgtgggcttg gttacagtct cgacaatctt tcactaatgg cgttgacgtt
     1801 ggcgatcggt tttcttgtcg acgatgcgat cgtgttcctg gaaaacactg tgcgtcgtat
     1861 cgagcgcggc gagccacctt ttgaagcgtc tatcaacagc gcgaaagaga tcagtttcac
     1921 gattgtctcg atgacgatct cgctcgcggc tgtattcata ccgctggtgt tcatgacggg
     1981 actcgtcggc cgaatctttc gcgagtttgc gatcacgatc gtcattgcga ttctcgcgtc
     2041 aggcctggtc tcgctgacac tgacgccgct gatgtgcgcg cgccttttga aagaacgcgg
     2101 ccacggctta aagaagacgt gggtggagcg cgttatcggc ggtatcgaga aacgcgtgct
     2161 cggtttgtat ggtggatcgc tctggtggtt cctgcgtcat cgctgggtct cactggtgat
     2221 ctgggtcgtc tgtttggtgg gcacagtctg gctgttcatg ctggtgccaa aaagcttcct
     2281 gccgccagga gacagtggtg ttatcttcgg cgcgtttatt gctcaggaag gctcgtcgcc
     2341 aaaacagatg cgggagtacc aggatcgcgt cgatcgaatg ttgcacgagg atccgaacgt
     2401 catcactgac ttcacgctga caggcgctac cggctttctc tcgtcaaaca caggcattac
     2461 ctttacctat attggaccac ctgaaaaccg caaacccatc gaggacgtaa ccgccgagat
     2521 gatgggcaag ctcaacacga ttccgggaat catgactttc ctgcgtccat ggccggtgcg
     2581 ggagatcagc actggtgtta caaatcaaac tcagggtcaa tatgcgttca ctgtttccgg
     2641 tgtgaatccg agccaggttt acgaaactgg tcaaaaacta ctggagaaac tgcaaccgtc
     2701 accgctcttt ctgagcgtct catccgacct ctacaacaac acaccgaacg tgaatatcga
     2761 cattcgccgt gaacaggcga aaacctacgg tgtttccgaa gctcgaattc tgacgttgct
     2821 gcgtaatgcc tactcccaaa attatctgta cctgatcaag cagcccgagg atcagtatca
     2881 ggttattctt gagatggacg attcagtgcg ggagaaacct gaggatcttt cgctgcttta
     2941 tattaagtca gacgacggcg cgcggcttat tccgcttagt gagctggtca cttggaaatc
     3001 cactcttggt ccacaatcag tgaaccactt caatcagttc accagcatga cgctctcgtt
     3061 caacctcaag cctggcgtgg ctctcggtga tgcgacggat ttcatcacta agtcggcgca
     3121 ggaggtagtt ccacagggcg tgcgcgcgga gctgcagggg gaggcgctga cgtttagccg
     3181 cacggttacc agtttgacga tcctgatggg gttggcggtg tttgtgatgt acgtgatact
     3241 cgcgatcctt tatgagagtt acgtgcatcc gttgactgtg ctttcaacgc taccgacggc
     3301 tctcgtcggc ggattgttga cgctgatcgt gtttggggcg gaggcgtcgt tgtatgcgtt
     3361 tgtcggcatg ttcatgttga tgggcattgt taagaagaac ggcattatga tcgtggactt
     3421 tgcgcgccat cgtgtcgagg cgggt
//
LOCUS       DASTEY010000484         7624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1213538, whole genome shotgun sequence.
ACCESSION   DASTEY010000484 DASTEY010000000
VERSION     DASTEY010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213538"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="VFO72_08485"
     CDS             <1..1573
                     /locus_tag="VFO72_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479367.1"
                     /translation="AQTPANVRLPGTSSSEEQITRLVAEGVAALERNDTAAASKLFQQ
                     VLALKPNHAAAHTFLGVLADRAGDLAAAQTHFEAAVSAEPTSPSARNNLGAVLLKRGS
                     TKQAAEQFEASLRLNKNQSQALFNLAQIRFLSGTSADLLAAREMFEQVQAIAPDAEIA
                     RSLVVVSLRLSDREAAAKYYRQYSDQLVGNTAVGTFANRSELGAALFEAGMLQEAEGE
                     LAAAVKMSPMDQPSILRLARVYIARKDLPAAGRILEGAVARGIEAAPVYALLSSVYEQ
                     SGHIENAIPAMRLAIERDPQSENYRFAYGMLLTNAMAPKAAVIRLTEAIDLYPASPRL
                     WLGLGIAHFTAGDNAEAVKAFKRSLELDPNYAPAYAYLGMTSVEVGNYADAVKTYERA
                     LAVDNKLGVVNYLLAEALLGLGGSHQTRIEENLKRAIELEPTFAPARLSLGKFYFRTG
                     RLSEAVSELEHVLKLDPQLAGAYYQLGLVYSRLNRKEQAQATMATFKRLNESRRTQER
                     EEVREIVRRLADVRF"
     gene            1603..2277
                     /locus_tag="VFO72_08490"
     CDS             1603..2277
                     /locus_tag="VFO72_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479368.1"
                     /translation="MFLIAFHIFIFLAGASAPQQQPAAPAQTAQPPIAEWRDDFEDEK
                     LDEAKWELFTFEGGSGGKIEVKNKQLKMRGAGVSRSGVRTKQSFHGDRFYVEAALAKV
                     GPRVPQPGEASFPPGYAIVTVLFDGNPNYRVEWLLRSDGILEAWQSFDGRLTRLDKGN
                     LATKEKTPQLGIARRGEKIYFMLNREVGLESSARGLSLDFKVMVYGFGSSENNWESVA
                     VQTLKQ"
     gene            2274..3944
                     /locus_tag="VFO72_08495"
     CDS             2274..3944
                     /locus_tag="VFO72_08495"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HET9479369.1"
                     /translation="MNRRQFLRGGMAIGSALLLERAGGLTYSSYAADSRIEVLLGEEI
                     ATISPHIYGHFAEHLGGVVYDGIWVGENSKVPNTGGIRNALVDALKRIKAPVIRWPGG
                     CFADSYDWRDGTGPRNQRPRRTNFWADAPEWPKNAPNGPWKFDTNHFGTNEFLRFCHL
                     SGAEGYLAVNVRGLGAQEFYEWIEYCNSPAGSTTPAERRASGESPSREPFRVRFWGVG
                     NETWGCGGNLTPEEYAAEYRRFTAAVPRYGVDLKFIASGANVDDYNWTRGFFSKTAEK
                     NRGLFNGIYGWGLHHYAWNLGLGRTNDWFAAKGDALKFNDAEYYELLREADRMESLIN
                     QHWTIMGEYDRQHRVKLVVDEWGAWYKPGSEVAPTHLLGQQSTMRDALLAGLTLDTFN
                     RHADKVAMANVAQLINCLHSLFLAHEDKFTVTPTFHIFEMFMPHMGAQAVRAVFSAPQ
                     VRYTRVDKPAEFWGLSGSASLNGKQLTLTVTNPHLTEVREAEIAVRGGRATAVQVRVL
                     SMPDVHAHNTFQNPAVVQPRNEAAALTGGTISYRFPPASVTRLQITVA"
     gene            3960..5000
                     /gene="galT"
                     /locus_tag="VFO72_08500"
     CDS             3960..5000
                     /gene="galT"
                     /locus_tag="VFO72_08500"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9479370.1"
                     /translation="MYWEERWHPLREEWVIIAAHRQNRPWSGETIEHNGEPLPAYVDD
                     CYLCPGNLRVGGARNDNYSSTFIFDNDHPCVGDDAPKILQKPPGIYHNQAANGIARVV
                     CYSPNHSLTLAELEPLEIENLLGVWQDQYRELSARKEIKHVLIFENKGEAVGVSNPHP
                     HCQIYATNFVFKTIETEARASQRHLAETGRVLFQDVIRAEKEDGRRIICENESAIAFI
                     PYFARYAYEVFVAPKTTHGSIATLSRDELRDFAALLKQVLVRFDNLWQMPFPYVMPLH
                     QAPTDGGDYSSFHFHIEFHPPLRRPSLLKYLAGPEIGGGNFLSDTAPEEKAEELRSQS
                     AIHYKQTVREQL"
     gene            4997..6034
                     /locus_tag="VFO72_08505"
     CDS             4997..6034
                     /locus_tag="VFO72_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479371.1"
                     /translation="MTRPATDARQLLAPIRRIHERVRDEVVAECQKSELDQLAAVADD
                     TVEGDTIYAIDRISEDVLVDLFEKELANLAPLVLIAEGIEGGKLVLPRGASESDAVWR
                     VVVDPIDGTRGLMYQKRSAWVLTGVARNRGKDSNLGDIELAVQTEIPLLKQHLSDVLW
                     AIRGEGSSGERFNRLTQARQPLALRPSRAKTIRHGFAMLARFFPGARDELAAIDEEIV
                     FGALGPVEPGKAHCFEDQYLSSGGQLYELMMGHDRFVADLRPLVESFLQRRGLALGIC
                     CHPYDVCTELIAREAGVIVTDEHGRALSAPLTIDADVAWVGYANEAIRREIEPLLHEA
                     LEKRGLGVQEC"
     gene            6318..>7624
                     /locus_tag="VFO72_08510"
     CDS             6318..>7624
                     /locus_tag="VFO72_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HET9479372.1"
                     /translation="MVYEVIRGNASHALDVTEFIAILNSLDAHPELRTFFDSNSDVVV
                     TRAPGRLDVMGGIADYSGSLVLELPIAEATFVALQKDDSRQLRIVSLLDGTTRMLTFA
                     MPLAHLERAGEPLGYDDAQRYFRLDVSEHWAAYVAGVFLVLMRELGTCFNQGARLLIS
                     SRVPEGKGVSSSAAIEVATMTAVAAAFDVDISPRQLALLCQRVENLVVGAPCGVMDQM
                     TSVCGEEHQLLEMRCQPAELLGTIKLPRELAVWGLDSGVRHSVGGGDYGSVRAGTFMG
                     YRIIAELAGLNVIRDDKRIVIDDPRWNGYLANLTPEEFAEQFAKQLPETMIGEEFLAR
                     YSGMTDTVTTVEPERTYQIRAPVAHAVYENSRVNVFANLLRERHEPLTRREKAALGKL
                     MYDSHASYSACGLGSPATDTLVELVRTAGVDQGLYGAKITGGG"
BASE COUNT         1681 a         2010 c         2250 g         1683 t
ORIGIN      
        1 ggcgcagact cccgccaacg ttaggctgcc cggcacttct tcttccgagg aacagataac
       61 tcgcttagtt gccgagggtg tcgccgccct cgaacgcaat gatactgctg cggcaagcaa
      121 gctcttccaa caagtgcttg cactgaagcc gaaccatgcg gcggcgcata ctttcctcgg
      181 cgtacttgca gatcgggctg gtgacctggc ggccgcgcag actcatttcg aagccgctgt
      241 ctcggcggaa ccaacttcgc cgtccgcgcg aaataatctt ggcgctgttt tactcaaacg
      301 aggctcaaca aaacaggcgg cggagcaatt cgaggcgtct ttgcggttga ataagaacca
      361 atcgcaagct ctttttaatc ttgcccagat tcgcttcttg tccggcactt cagccgactt
      421 gcttgcggcc cgcgaaatgt tcgaacaggt tcaagcgatc gcgcccgatg cggagattgc
      481 gcggtcgctg gtggttgttt cgttacgact gagcgatcgc gaagcagcag cgaaatacta
      541 tcgccagtac tcggaccaat tggtcggtaa cacggcggtc ggaacgtttg ctaatcgaag
      601 cgaactcgga gccgcgttgt ttgaagccgg catgttgcag gaagctgaag gagagttggc
      661 agccgcggta aagatgtcgc cgatggacca gccctcgatt ctgcgtctgg cccgtgtcta
      721 cattgcacgc aaggatttac ccgccgccgg gcgaattctc gaaggcgcag tagcgcgcgg
      781 tatcgaagcg gctccggtgt atgcgttgct gtcctcggtt tacgaacaga gtggtcacat
      841 tgaaaacgcg attcccgcaa tgcggttggc catcgagcga gatccgcagt ccgaaaacta
      901 ccgtttcgcg tacggcatgc tgttgacgaa tgctatggcg ccgaaggccg cagtgattcg
      961 gctaacggag gccatcgatc tttatccggc ttctcccagg ctctggctcg gactcggtat
     1021 cgcgcacttc acagcgggtg acaatgctga agcagtaaaa gccttcaaac gttccttaga
     1081 gctcgatcca aattacgcgc ctgcgtatgc gtatctggga atgaccagtg tcgaagtggg
     1141 taactacgcc gacgcagtta agacgtacga gcgggcactg gcggtcgaca acaagctcgg
     1201 cgttgttaac tacctgctgg ccgaagcttt gctggggctt ggtggatcgc accagacacg
     1261 catcgaggaa aatttgaagc gcgcgattga gttggagcca acatttgcgc cagcccggct
     1321 gtcgttaggg aagttctatt ttcgcactgg ccgcttatct gaagccgtga gtgagcttga
     1381 acatgtcctc aagctggatc ctcaattggc aggagcttac taccaactcg gcctggtgta
     1441 ttcacgcttg aaccgcaagg agcaggcgca ggcaacgatg gcgacgttca agcggctaaa
     1501 cgaatcgcgt cgaacgcagg aacgtgaaga agtacgcgag atagttcgcc ggcttgccga
     1561 cgtgcgtttc tgaggctcaa aaatcacgac ccgtaaaaag cagtgtttct gatcgcgttt
     1621 catatcttta tatttctggc aggtgcatct gcgccgcagc aacaaccggc cgcaccagct
     1681 caaaccgcgc agcctcccat cgccgaatgg agagacgact tcgaagacga gaagctcgac
     1741 gaagcgaaat gggaactctt cacttttgaa ggaggcagcg gcggcaagat tgaggtcaag
     1801 aacaaacaac tgaagatgcg tggcgcgggt gtttcccgtt ccggtgtgcg caccaaacag
     1861 tcctttcacg gcgatcgctt ttacgttgaa gcagcgctcg cgaaagttgg accgagagtg
     1921 ccgcagccgg gcgaggcttc ctttccaccg ggatatgcca ttgtgacggt gctgttcgat
     1981 ggcaatccta attaccgagt tgaatggttg ttgagaagtg atgggatcct ggaagcgtgg
     2041 cagagcttcg acggccggct cacacgcctt gacaaaggca atctggcgac aaaggaaaag
     2101 actccacagt tgggcatagc gcgtcgcggc gaaaagatct atttcatgct caaccgggaa
     2161 gtgggcctcg agagctcggc ccgtggtttg tcgttggatt ttaaagtgat ggtttacggt
     2221 tttggttctt ccgaaaacaa ctgggagtca gtagcagtgc aaactctaaa acaatgaaca
     2281 gacgtcaatt cttacgcggt ggaatggcga tcggatcggc gctattgctg gaacgcgctg
     2341 gtggtctaac ctactcgtcc tacgctgctg actcgcgcat cgaagtgctt ttaggcgaag
     2401 agatcgccac gatcagccca cacatttacg gccactttgc cgaacatctc gggggcgtgg
     2461 tttatgacgg tatctgggtc ggcgagaact caaaggtccc aaacacaggc gggattcgca
     2521 acgctctcgt tgacgcgcta aaacgaatca aagctccagt cattcgttgg cccggaggct
     2581 gcttcgcaga cagttatgac tggcgcgatg gaactggacc acgcaatcaa cgtccaagac
     2641 gcactaactt ttgggctgac gcacccgaat ggcccaaaaa cgctcccaat gggccatgga
     2701 agtttgatac caatcacttc ggcacaaacg aatttcttcg tttctgccat ttgtcaggag
     2761 ccgaaggcta tctggcggtg aatgttcgcg gtcttggagc gcaggagttt tacgagtgga
     2821 ttgagtactg caactcaccg gccggttcga cgacccccgc tgagcggcgc gcgagcggtg
     2881 agagtccgag tcgcgaaccg tttcgtgtcc gtttctgggg agtaggtaac gagacctggg
     2941 gttgcggtgg caacctgact ccggaagagt acgccgcgga ataccgaagg tttactgcgg
     3001 ccgtgccgcg ttacggagtc gacctgaagt tcatcgcgtc cggcgcaaac gtcgacgatt
     3061 acaattggac gcgcggtttc ttctccaaaa ccgcagaaaa aaatcgcggc ttgtttaatg
     3121 gcatctacgg ctggggactg catcattacg cctggaatct gggcctcggc aggaccaacg
     3181 actggtttgc cgctaaaggc gacgccctta agttcaacga cgcggaatat tacgaattgc
     3241 tgcgcgaggc cgatcgcatg gagtcgctga tcaatcagca ttggaccatc atgggcgaat
     3301 acgatcggca acatcgggtc aaactggttg tcgatgagtg gggagcgtgg tacaaacccg
     3361 gatcggaagt ggccccgacg cacttgcttg gccaacaatc aactatgcga gacgcgcttc
     3421 ttgccggcct cacgctcgac acgtttaatc gtcacgctga caaagtggcg atggcgaatg
     3481 tggcccaact aatcaattgt cttcattcgt tgtttctggc gcacgaagac aagttcaccg
     3541 tgacgccgac gtttcatatt tttgaaatgt tcatgccgca catgggggcg caggctgtcc
     3601 gcgccgtgtt cagcgcaccg caggtccgat acacacgtgt tgacaagccg gcagagttct
     3661 ggggtttgtc aggctcggca tctcttaatg gaaaacaatt gaccttgact gtgacgaacc
     3721 cgcacctgac tgaagttcgt gaagcagaga tcgcagtccg cggtggacgg gccactgccg
     3781 tgcaggttcg cgtgctttcg atgcccgacg ttcacgcaca caacacgttc caaaatcctg
     3841 cggtggtcca accgcgcaac gaagccgccg cattgacggg tggcacgatc tcttatcggt
     3901 ttccgcctgc ttcggtaacg cgcctgcaga tcactgtagc ctgagcgcca taaaggaata
     3961 tgtactggga agagcgttgg catccattgc gcgaagagtg ggtcattatt gcggcccacc
     4021 gtcagaatcg tccgtggagc ggcgagacga ttgagcacaa tggagagccg ctgcccgcct
     4081 acgtcgatga ctgctacctg tgtcccggca acttgcgtgt cggcggcgcg cgaaatgaca
     4141 actacagcag cacgtttatt tttgacaacg atcatccctg cgtcggcgac gatgcgccta
     4201 agatcctcca aaaaccaccc ggcatctacc acaatcaagc tgcgaatggc atcgcgcggg
     4261 tcgtctgtta tagcccaaat cacagtttga cattggcaga actcgagccg ttggagatcg
     4321 aaaatctatt gggagtgtgg caggaccagt atcgagaact aagtgctcga aaagagatca
     4381 agcacgtact gatctttgaa aacaaaggtg aagcagtagg agtttccaat ccacacccgc
     4441 actgtcagat ctatgcgacc aacttcgtct tcaagaccat cgagaccgaa gcacgcgcga
     4501 gccaacggca tctcgcagaa acggggcgcg tgctctttca ggacgtgatt cgagccgaaa
     4561 aggaagatgg ccgccgcatc atttgcgaga acgaatcagc catcgctttc attccttact
     4621 tcgcgcgcta cgcgtatgag gttttcgtgg cgccaaaaac tacgcatggc agcatcgcta
     4681 cgctatcccg cgatgaactt cgcgactttg cggcactgtt gaaacaagtt cttgtcaggt
     4741 tcgacaacct ctggcagatg ccgtttcctt acgtgatgcc tctgcaccag gcgccaaccg
     4801 atggcggcga ctattcgagt tttcacttcc acatcgagtt tcatccgcca ctgcgccgcc
     4861 ccagtttgct caagtatctg gccggaccgg aaatcggcgg cggcaacttc ctttctgaca
     4921 cggcgccgga agaaaaagca gaagaactac gttcgcagtc ggcaattcat tacaaacaaa
     4981 ctgtgcggga gcaattatga ctcgtcctgc aacagatgcc aggcagctgc tcgcgccgat
     5041 tcgtcgcatt catgaacgcg ttcgagatga agtggtggcc gagtgtcaga aaagtgagtt
     5101 ggaccaactc gcggcagtcg ctgacgacac cgtggagggc gatactattt atgcaataga
     5161 tcgcatcagt gaagacgttc ttgtcgatct gtttgaaaag gagctcgcca atttggcgcc
     5221 tctcgtgctg attgccgaag ggatcgaggg cggaaagttg gtgttgccgc gtggcgcctc
     5281 ggaaagcgat gcggtttggc gcgtcgtcgt ggatccaatc gatgggacgc ggggcttgat
     5341 gtatcagaaa agaagtgcgt gggtgttgac aggggtcgcc agaaatcgcg gcaaagatag
     5401 caacctcggc gatatagagt tagcggtcca aacggaaata cctttgctaa agcagcactt
     5461 gagcgatgtg ctctgggcca ttcgcggtga aggttcgtct ggcgaaagat ttaatcgtct
     5521 gactcaagct cgacagccgc ttgcattgcg accttcgcga gcgaagacga ttcgccacgg
     5581 ttttgcgatg ctcgcacggt ttttcccggg cgctcgtgat gagctggcag caattgatga
     5641 agagatcgtg tttggtgcgc tggggccggt cgagcccggg aaggcgcact gcttcgagga
     5701 tcagtatttg tcttcgggtg gacagctcta cgaactgatg atgggccatg atcgctttgt
     5761 tgccgacttg cgtccgcttg ttgagtcgtt cctgcaacgt cgcggcctgg cgctcggcat
     5821 ctgctgccac ccttacgatg tttgtaccga actaatcgcg cgcgaggccg gtgtgatcgt
     5881 caccgacgag catggtcggg ccttgagcgc gccacttacg atcgatgccg atgttgcgtg
     5941 ggttggatat gcgaacgagg caattcgtcg tgagatagaa ccactgttgc atgaggcgtt
     6001 ggagaagcga ggcctcggcg ttcaagaatg ttagcctgcg caagtgctcc ggaggagcat
     6061 tatgtttata gctcttgtcc ccgtggtact tgcgctccag cggagcgcaa tatgtttttg
     6121 aagcatcgag ttacatgtcg ctcctgacgg agcgacagac tttggtttgt gggtggctat
     6181 agacatcttg ctcctccgga gcattcaggc gcgacaaaga agggtgccgg ggtgcgtcac
     6241 cagaattttg tatccgctac gtacttttcg ggcaacgcct gttgaaccta aagcggtacg
     6301 cacgggcgca cgtaaatatg gtgtatgaag ttatccgagg taatgcctcg cacgctctcg
     6361 atgtaactga gttcatcgcg atcttaaata gtctcgatgc ccatcccgaa ctgcgtacgt
     6421 tctttgactc gaattcagat gtcgttgtca cgcgtgctcc gggtcgactc gacgtgatgg
     6481 gcggcattgc cgactactcc ggctcgctcg tgctcgagtt gccgatcgcg gaagcaacct
     6541 tcgtggcgtt gcaaaaggac gactcgcgac aattacgtat cgtcagtttg ttggacggca
     6601 cgacgcgaat gctgacgttc gccatgcctc tcgcgcactt ggagcgagct ggcgagccgc
     6661 ttggttacga cgatgctcag cggtatttcc ggctggacgt cagcgaacat tgggcagctt
     6721 acgtagcggg tgttttcctc gtgctgatgc gtgagttggg cacatgcttc aatcaaggcg
     6781 cacgattgct tatttcgtcg cgtgtgcctg agggcaaagg tgttagttcg tccgcggcga
     6841 tcgaagtagc gacgatgacg gcagtagcgg ccgctttcga cgtcgatatt tcgccacgac
     6901 agttggcgct gctttgccag cgagttgaga acctggtcgt tggcgcaccc tgcggcgtga
     6961 tggaccagat gacctccgtt tgcggtgaag aacatcaact gctcgagatg cgctgccagc
     7021 ccgcggaact gctgggaacg atcaagttac ctcgagagct cgcggtctgg ggccttgact
     7081 cgggtgtgcg gcattcggtt ggcggcggtg attacggatc ggttcgggcc ggaacgttca
     7141 tgggttaccg aatcattgcg gaattggccg gcttgaatgt tatccgcgac gacaagagga
     7201 ttgtcattga tgacccgcgt tggaatggct acctggcgaa tctgacgccg gaagagtttg
     7261 cggagcaatt cgcgaagcaa ttaccagaga caatgatcgg agaggagttc ctcgcgcgtt
     7321 atagcggcat gacagacaca gttacaaccg ttgagcccga acggacatat cagattcgcg
     7381 cacctgtggc ccatgcggtc tatgagaatt cacgagtcaa cgtttttgcg aacctgctgc
     7441 gcgaaagaca cgagccgctt accaggcggg aaaaagcagc gctcggcaaa ttgatgtacg
     7501 actcgcacgc gagttactcc gcttgcggcc tcggttcgcc agccacagat acgctggttg
     7561 aattggtgcg gaccgcggga gtggaccagg gactgtacgg tgcaaagatt actggcggcg
     7621 gaag
//
LOCUS       DASTEY010000485         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_719543, whole genome shotgun sequence.
ACCESSION   DASTEY010000485 DASTEY010000000
VERSION     DASTEY010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719543"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="VFO72_08515"
     CDS             <1..1766
                     /locus_tag="VFO72_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9479373.1"
                     /translation="MGMDEGIGVDFLGAGNCLGLEGVNKPETWKYSASLMARTEVLEI
                     SLKDFSTDPELRHQLFDVLSSFSNADDALPLTLESVVDVNALKSAEESIATGIVDGRN
                     VLVMDMDLCVRCGNCSLACHKMHGQSRLLRRGIQIERPVAIGKKRLQHALVPQVCMHC
                     ADPECMTGCPTGSIFRDPRGQVDIDRVTCIGCFDCATQCPYDAISMVPREPAANGRGF
                     MSKLARVFSFRTDEAPAPEAADDVVAIKCNLCEGTSLNPAGARRQAYSCEENCPTGAL
                     VRVNPVEYFEEVKSARGLVFRDETHAVGRNIHKSDPLARAWHVAGLLATILSLAGVVW
                     GLARFGYDEVLAGAWLTMRWLTGIVGLVGVAVVMTYPFRKQVYRRRVGALRYWMLVHV
                     YAGVVAGIVLLLHAGSSLGGLLTSLLYVVFDLVILSGVVGVVTYFVAPRIMTRIEGEP
                     LLVEDLEARREELRQQATSVLEQSEGWLKEEIRDRVYPRFLSRAFLWRQLSRREELKS
                     LLAQAREEYKSQTTRTATHEERELLLSAVETAVTLRRVDALLLLHRALRSWIPIHVVS
                     TAAMLALMVVHIVQVVFFNAR"
     gene            1756..>2058
                     /locus_tag="VFO72_08520"
     CDS             1756..>2058
                     /locus_tag="VFO72_08520"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9479374.1"
                     /translation="MPVNNQYIIIRQDLQIDPVTVITEGLLIGRLNKCEVLLNHPSVS
                     RVQAGIKQFDNAYYIFALRPKNPVILNGRPVEDNEALAAGDIIEVGAFQLEIDTTEE"
BASE COUNT          411 a          530 c          645 g          472 t
ORIGIN      
        1 tcatgggcat ggacgaaggc atcggcgtgg acttcctcgg cgccggcaat tgtctgggtc
       61 tcgaaggagt aaacaaacct gagacgtgga agtacagcgc cagcctcatg gcgcgaacag
      121 aagttctcga gatctcgcta aaagattttt ctaccgatcc agaattacgt caccagctct
      181 tcgatgttct atcaagcttc tccaacgcag acgacgcgct cccgctcacc ctcgaatccg
      241 tcgtcgacgt caacgcactc aagtcagccg aagaatcgat tgctacggga atcgtagacg
      301 gtcgcaacgt gctggtgatg gatatggatc tctgtgtccg ctgcggcaac tgctcgctgg
      361 cgtgtcacaa gatgcacggg cagtcgaggt tgttgcgccg cgggatccag atagaacgac
      421 ctgtggctat cggaaagaaa cgtttgcagc acgcgttggt gccgcaggtg tgcatgcact
      481 gcgcagatcc ggagtgtatg actggttgcc cgacgggttc catcttccgc gatccgcggg
      541 gccaggtgga tatcgatcgc gtgacgtgca tcggttgttt cgactgtgcc acgcagtgtc
      601 cgtacgacgc gatttcgatg gtgccgcgtg aaccggctgc gaacggtcgc gggtttatgt
      661 cgaaacttgc acgcgtgttc agttttcgca ctgacgaagc gcccgcgccc gaggctgccg
      721 acgacgtcgt ggcgatcaag tgcaatttgt gcgaaggaac atcgttgaat ccggccggcg
      781 cgcgccgtca ggcgtattcg tgcgaagaga actgcccgac cggcgcgttg gtgcgggtga
      841 atccggttga atacttcgaa gaggtcaagt ctgcgcgcgg gcttgtgttt cgcgatgaaa
      901 ctcacgcagt cgggcgcaac attcacaaga gcgatccttt ggcgcgagcg tggcacgtcg
      961 cgggactgct ggccacgatc ctttcgctcg ccggtgtcgt ttggggactc gcgcgattcg
     1021 gatatgacga ggtattggca ggcgcgtggt taacgatgcg ctggctgacc ggcatcgtcg
     1081 gactcgtggg cgtcgccgtg gtgatgacgt atccgtttcg caagcaggtt tatcgacgtc
     1141 gtgttggcgc gctgaggtat tggatgctgg tccacgtcta cgccggtgtt gtggccggta
     1201 ttgttctgct gctgcacgcc ggctcgagtc tcggcgggtt gctgacgagt ttgttgtacg
     1261 tagtcttcga tctggtgatt ctgtcaggcg ttgttggggt cgtcacttat tttgtggcgc
     1321 cgagaattat gacgcgcatc gaaggcgaac cgttactcgt cgaagatctc gaagcgcgcc
     1381 gcgaggaact acggcagcag gcaacatcgg ttctcgaaca gagcgaaggc tggttaaaag
     1441 aggagatacg cgaccgagtt tatccacggt tcttgtcgcg cgcgttcctg tggcgacaac
     1501 tttcgcgtcg cgaggaacta aaatcgttgt tggcccaggc gcgcgaggag tataagtcac
     1561 aaacaacacg aacggcgaca cacgaagaac gagagttgtt gctgtcggca gttgaaactg
     1621 ccgtcacgct gcggcgggtt gatgcattgt tgttgcttca tcgagcgctt cgttcgtgga
     1681 ttccgattca tgtggtgtca acggcggcga tgttggcctt gatggtcgtt catatcgtgc
     1741 aagtagtctt cttcaatgcc cgttaacaat caatacatca ttatccggca ggacttgcag
     1801 atcgatccgg tgacggtcat cacggaagga ctgctgatcg gacggctcaa caagtgtgaa
     1861 gtgttgttga atcacccgtc agtgtcgcgc gtacaggcgg gcatcaagca gtttgacaac
     1921 gcgtattaca tcttcgcgtt gcgtcctaag aatccggtca tcctcaacgg cagaccggtc
     1981 gaggataacg aagcgctcgc cgctggggac atcatcgagg ttggagcatt tcaactcgaa
     2041 attgacacga ctgaggag
//
LOCUS       DASTEY010000486         6689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1592691, whole genome shotgun sequence.
ACCESSION   DASTEY010000486 DASTEY010000000
VERSION     DASTEY010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592691"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFO72_08525"
     CDS             complement(<1..577)
                     /locus_tag="VFO72_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479375.1"
                     /translation="MIHRPRRLRRNHALRSLVRETRLTADNFVLPLFACPGEGVRREI
                     SSMPGVFNLSVDEIAQEAAAAYDTGVRSIILFGLPEAKDELASGAYAEDGIVQRAIRA
                     IRRSSPELIIIADTCLCEYTSHGHCGVVRGGEVLNDESLELLARTAASQAAAGADIVA
                     PSAMMDGQVGAIRETLDESGFENTAIMSYAVK"
     gene            complement(588..1046)
                     /locus_tag="VFO72_08530"
     CDS             complement(588..1046)
                     /locus_tag="VFO72_08530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9479376.1"
                     /translation="MKSIQETYAPNLACFGCGPANAKGLRIRSFADGDEVVAEWQPSR
                     EHEAFVGMLSGGIIGTLLDCHCNWTAAWHLMKKSGTDTPPCTVTSEYAIKLLRPTPTD
                     RPVRLVARVVESTEDRATVAGELIAHDKVCATCRGTFVAVKPGHPAYHRW"
     gene            complement(1107..2609)
                     /locus_tag="VFO72_08535"
     CDS             complement(1107..2609)
                     /locus_tag="VFO72_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET9479377.1"
                     /translation="MNLFEAVFDHDLAAPAINFGGREMSYGELRTATLQMAQAISSFG
                     IERGDRVALLLHDSPEFVEAFIATCSLGAIAVPINMALRLDEQCAILHNCSATLALTE
                     PDLCSTLPTHAPEKLHSLKNIVSDISHWQLPIDNWQSIQFPAPRDNEPAFILYTSGST
                     GEPKGAMHRQADIFYTNQTYCREVLRLTVHDRLFSSSRLPFAYGLGNSFTFPLLNGAT
                     SILCREKPTPEIIARIFAEYRPTIFFGVPVVYNLLLEHHRQNQQLDCSSLRLCISAGE
                     ALPAHLGEQWEKAFGVQLLDGIGSTEILHMFMSNHEHEVRYGSSGRLLEGYQARLVDE
                     NGQATPDDVEGNLWIKGDSAALGYWEKPEQTARTFVDGWVRTGDLYRRDRDGYWFHMG
                     RSDDCFKSSGQWVSPVEVEGVLLRHPKVARAAVVEDFDAGGLSCACAFVVAQELESDP
                     RSLEQELRESAAAALPRFKQPRRYLFVSELPYTATGKVQRFKLRQALRVK"
     gene            2676..>6689
                     /locus_tag="VFO72_08540"
     CDS             2676..>6689
                     /locus_tag="VFO72_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479378.1"
                     /translation="MELILLLILLLFFTFAVIGVLASAVTLARWFTKPNPPPRVYEGP
                     DDDLAATERQIVRFYTDGKITDEVYEHLMARIRAERTPSTTDTTDKTDSVEEVVESVP
                     PVVIDIDREPPTEPIPVPPTPRYEPPPPRPPRRPFSEVLNSFMEESNIRWGEIIGGLL
                     IIGCSTALVVSLWAQISQIPVLKFLIFTTVTAVLFGVGLYTEHRWKLPTTSRGILTIA
                     TLLVPLNFLAIAAVSASNTPGALAIASELAAPAIFLCLVYFAGRVITPGCAHILSAGV
                     LGSSIGQLLVRHFASSDSSPALLVLLAAFPVACYVVTVALSLRLVLADREIDESETTT
                     VFTILGTMSFAALLPFGLLLYKSGPLNLTMMYVAPIVTLWGLPMLATGTVLWRRIKNK
                     ELVASRTTGTAIGILGVMIVLAGMVLAWPNPASIVPAALLNFAIFTALAIALELPVAH
                     LIAANCLALAYLVLFQVFAGHVAWKNLRVMSLLEVTLSLSSGQALAGIFVIFLIAAEW
                     LERKRRTRDSVYYLVAAGLIAIVSLTLTAFIGFWVPENHWLWIVFAIYSLGAFWIAWR
                     QKESGFTWIGSALLLFALVDAFSRTLSLSFPWQTALLAHATICAAAAIVVSRTARFRL
                     FSKPLNDSALITLVMGIVALFQANTWEVTSMQAQRVFWISGILALLLWLNRRRIHFSA
                     FQIALICAVVLTVKALQQFEWYSYFPLAFLHPSALQIYGTVLALFCLMWIGLRFAVRR
                     GLPKDDTAAQDGWLRDAYRLLDTDYSIDRVLSWLLLGAFLLFAFYGSLSGLVRELTSL
                     GSSYPGLNIAGFPHQEALALGSWILLGLLTVLMLANFRERRQEEYLLGAVLVLSTAIP
                     LLAGTFEPQMATAAAWRWLAALFLLGGSIVLWYRRDGTDRMRLLLIALTVAPLLLFTI
                     GPALRAIYYLPVQGPTAGIFAWFADEVLYGGPLIIVALVMIGYAMRERLPEYSFYGAL
                     LINLTVTLVFLLAVVSGKGVMDRVVLVRLVQLNAITFAVYALPWLSTRRLWQQKLDER
                     RQTRADDLLELQLGLAIGLNLFLIVPVALGLIIEPEPPGIGTIAAGTLLGWLAFATTA
                     TAVAWLTRIRSRDLSAVLFAGGLMAVSCLFAFTVADTSGWLALHVLTVCVTLSAWLML
                     VAADRDARWQRQCRQLAAVSGSLAAFLSVRTATDLDATGWWSVGPLVSLSILFAVLNW
                     HTLRRRYLYVAGVLFTFAASIWCVRILPDSFEEGFVAINVAAGCLAGIVLLWLELRAR
                     RLTDTETPQSTVLSFHNLSAIGWPLLLAFLVAISPGVARFNSPVATMPGLHWIAMAAT
                     IALMTAVLWDEHAKYAVA"
BASE COUNT         1296 a         1937 c         1890 g         1566 t
ORIGIN      
        1 acttcaccgc gtaagacatg atggcagtgt tttcaaaacc tgattcgtcc agcgtctccc
       61 ggattgctcc gacctgccca tccatcatcg ctgacggcgc aacgatatcg gcgcctgccg
      121 cggcctgact tgccgccgtg cgcgcgagca gttccagcga ttcatcgttc aaaacctcgc
      181 cgccgcgtac caccccgcag tgcccgtgcg aagtgtattc gcataaacac gtgtcggcga
      241 tgatgatcag ttcaggtgaa gaacggcgaa ttgcgcgtat cgcgcgttgc actattccat
      301 cttcagcgta agcgcccgag gcgagttcgt ctttcgcttc aggcagccca aaaagaatga
      361 tgctgcgaac accagtgtca taagccgcgg ctgcttcttg cgcgatttcg tcgacggaca
      421 agttgaagac acccggcatc gacgagatct cgcgacgcac gccctcgccg ggacaggcaa
      481 acaacggcag cacaaagtta tcggcggtga gacgcgtttc gcgaacgagc gagcgaagcg
      541 cgtggttacg gcgaagtcta cgaggacgat ggatcatgat cttgcagtta ccatctgtga
      601 tacgcaggat gtcctggctt aacggccacg aacgttccgc gacacgtagc gcaaactttg
      661 tcgtgcgcga tcaactcgcc tgcgacagtt gcgcgatctt cagtcgattc cacgacgcga
      721 gcgacaagtc gcaccggtcg atcggtcggc gttggccgca aaagtttgat ggcatactct
      781 gatgtgacag tgcaaggcgg cgtatcggta ccgctctttt tcatcaggtg ccacgcggcg
      841 gtccagttgc aatgacaatc gagcagcgtg ccgatgatgc cgccgctcaa cattcctaca
      901 aacgcctcgt gctcacggct tggctgccac tccgccacga cctcatcacc gtcggcaaag
      961 ctgcgaatgc gcaagccctt cgcgttggct ggaccacacc caaagcacgc gaggttagga
     1021 gcgtaagttt cttgaatgct tttcatctgt ttttgtactt tgtcagtagt ccgtggtcag
     1081 ttgtcacaaa gtacaaagca caaagttcac ttgacacgca acgcctgccg caacttaaat
     1141 cgttgcactt tccccgtcgc cgtgtacggc aactctgaaa caaacagata ccgtcgcggt
     1201 tgcttgaaac gcggcaacgc cgccgcagcc gactcgcgta actcctgttc caggctccgc
     1261 gggtcggatt ctaactcctg agcgacaaca aaagcacacg cacacgacaa tccaccggca
     1321 tcaaagtcct caaccaccgc tgcccgcgcc accttcggat gccgcaacag caccccttcc
     1381 acttccaccg gcgaaaccca ttgcccgctc gacttgaaac aatcgtcact gcggcccatg
     1441 tgaaaccaat agccatcgcg atcgcgacgg tagagatctc ccgttcgcac ccagccgtcg
     1501 acgaacgtgc gggcagtctg ttccggcttt tcccagtaac ccagcgcggc gctatcgccc
     1561 ttgatccaca gattgccctc gacgtcatcc ggcgtcgcct gaccattctc atcaaccagc
     1621 cgcgcctgat aaccttcgag caaacggccg ctgctgccgt aacgtacctc gtgctcgtgg
     1681 ttcgacatga acatgtgcaa gatctccgtc gaaccgatgc cgtccagcaa ctgcacgccg
     1741 aaggcctttt cccactgctc gcccaagtgc gccggcagcg cttcaccggc ggagatacac
     1801 agccgcaagc tcgagcaatc gagttgctgg ttctgccggt gatgttcgag caaaaggtta
     1861 tagacaacag gcaccccgaa gaagatcgtg ggacggtact ctgcaaagat gcgtgcgatg
     1921 atctcaggtg tgggcttttc gcgacagaga atcgaagtcg cgccgttcag cagtggaaac
     1981 gtgaagctgt ttccgaggcc ataagcaaaa ggaagtcgcg aagaagagaa gagacgatcg
     2041 tgcaccgtca gacgcagcac ttcgcggcag taggtctggt ttgtgtagaa gatatcggcc
     2101 tgacggtgca tcgcaccttt aggctccccc gtgctgccgg aggtgtaaag tatgaaagcc
     2161 ggttcgttgt cgcgaggcgc gggaaactgt atggattgcc aattgtcgat tggcaattgc
     2221 caatgggaaa tgtccgaaac gatatttttc aacgagtgca gtttttctgg cgcatgtgtc
     2281 ggcaaggtgc tgcacagatc gggctccgtg agggccaatg ttgcactaca gttgtgaagg
     2341 attgcacact gctcatccag ccggagcgcc atgttgatgg gaacagcgat tgcgccgagc
     2401 gaacacgtgg cgatgaaggc ttctacaaac tcgggagagt cgtgcaaaag cagcgcaacg
     2461 cggtcgccgc gctcgatgcc gaatgaggag attgcctggg ccatctgtaa agttgccgta
     2521 cgcaactcgc cataagacat ttccctgccg ccgaagttga tcgcgggcgc ggccaggtca
     2581 tgatcaaata ctgcttcgaa caggttcatt gcggaagtta tactgcaatc gggcccagat
     2641 ggtcacccgg catccccaac cgacggtgag tcctgatgga acttatactg ctgctaatcc
     2701 tgctgttgtt ctttactttc gccgtcatag gcgtgcttgc ctctgcggtg actctggcgc
     2761 gctggttcac gaaaccaaac ccacctccgc gcgtatatga gggccctgac gacgatctcg
     2821 cagcgacgga acgccagata gtccgctttt acaccgacgg caagatcacc gacgaagttt
     2881 atgagcacct catggctcgt attcgcgccg agcgaacgcc ttcgaccacg gacaccacgg
     2941 ataaaacgga ctcagttgaa gaggtcgttg aatccgtgcc acccgttgtc attgacatcg
     3001 accgtgagcc acccacagag ccaatccccg tccctccgac gccacgctac gaaccgccac
     3061 caccgcgccc gccgcgcaga ccattcagcg aggtgctcaa ctccttcatg gaagagagta
     3121 acatccgttg gggcgagatc atcggcggcc tgctgatcat cggctgttcg acagcgctcg
     3181 tcgtcagtct ctgggcgcag atctcacaaa ttccggtgct gaagtttttg atcttcacca
     3241 ccgtcacggc agtcctcttc ggcgtcggtc tttacacgga acatcgctgg aaactcccca
     3301 caaccagccg cggcattctt acgatcgcca cactactcgt gccattgaac tttctcgcga
     3361 ttgcagcagt ttcagccagc aatacgccag gcgcactcgc catcgccagt gagttggctg
     3421 cgccagcaat cttcctgtgc ctggtctatt tcgccggacg cgtcatcaca cccggctgcg
     3481 cgcacattct ttcggcaggc gtgctcggct cttcaatcgg acaactgctt gtacgtcact
     3541 tcgcgtcgag cgattcttcg cctgcgctac tggtccttct cgcagcgttt ccagtcgcgt
     3601 gttacgtcgt gacggttgca ctttcactgc gcctggtgct tgcagaccgc gagatcgacg
     3661 agagtgaaac gacaacggtc ttcacgattc ttggcacgat gagtttcgcc gcgctgctgc
     3721 cgtttggatt gttgctctac aagtcaggac cactgaattt gacgatgatg tacgtcgcgc
     3781 cgattgtgac tttgtggggc ctgcccatgc tcgcgactgg cacagtgttg tggagacgaa
     3841 tcaaaaacaa ggaactggtc gcctcgcgca cgacgggaac ggcgatcggt attctgggcg
     3901 tcatgatcgt gttggccgga atggtgctcg catggcccaa tccggcgagc attgtgccgg
     3961 cggcgttact caactttgct atctttacgg cgctcgcgat tgcgctcgag ttgcctgtag
     4021 cccacctgat cgccgccaac tgtctggcgc tggcttacct ggtgctgttt caagtgttcg
     4081 cgggacacgt cgcctggaaa aatctgcgcg tcatgtcttt gctcgaggtg acgctttcac
     4141 taagcagtgg ccaggcgctc gccggcattt ttgtgatctt tctgatcgcg gctgagtggc
     4201 tcgagcgaaa gagacgtacc cgcgacagcg tttattacct ggttgcggca ggcttaattg
     4261 cgattgtgag cctgactttg acggcattta tcggattttg ggttcctgag aaccactggc
     4321 tgtggatcgt atttgcaatc tattcgctcg gcgcgttctg gattgcgtgg cgtcagaagg
     4381 agtcgggctt tacgtggatt ggatcggcgc tgctgctctt cgctctggtc gacgctttca
     4441 gtcgaactct ttcactttcg ttcccgtggc aaacagcgct gctggcccac gcgactattt
     4501 gcgccgcagc cgccattgtc gtttcgcgca cagcgcgctt tcggcttttt tcgaagccgc
     4561 tcaacgatag cgcgctgatc acgctggtaa tgggaatcgt ggcgctgttt caggcgaaca
     4621 cctgggaagt cacttcgatg caggcgcaac gagtcttctg gatctcgggc atcctggcgc
     4681 ttctgctgtg gctcaatcgc cgtcgcatac acttcagtgc gtttcagatc gcactaatct
     4741 gcgccgtggt gctgactgtc aaagcgttgc agcagtttga gtggtactca tactttccgc
     4801 ttgcgtttct gcatccgtcc gcattgcaaa tttatggcac agttctcgcg ctgttttgtc
     4861 tgatgtggat tggtttgcgt ttcgcggtgc gacgcgggct gccgaaggac gataccgcag
     4921 cgcaagatgg ctggttacgc gatgcgtatc ggctgctcga cactgactat tcgatcgatc
     4981 gcgtactctc gtggctgctg ctcggcgcgt tcctgctgtt cgcgttttac ggatcacttt
     5041 ccggtctggt gcgagaactg acatcacttg gcagcagcta tcccggtttg aacatcgccg
     5101 gattcccgca ccaggaagca ctggcgctcg gctcgtggat tctccttggt ctcttgacgg
     5161 ttttgatgtt ggcaaacttc cgggaacgcc ggcaggagga atacctgtta ggcgcggtgt
     5221 tggtgctttc gactgcgata ccgctgctgg ccggaacatt tgagccacag atggcaaccg
     5281 ccgcagcgtg gcgctggctg gcagcgttgt ttttgttggg cggttcgatc gtgctttggt
     5341 accggcgcga cggaacagac cggatgcgtc tgctgctgat cgcactgacg gttgcgccgc
     5401 tgttgttgtt cacgatcggt cccgcgttgc gcgcgatcta ctacctaccc gttcaaggtc
     5461 caacggcagg aatcttcgcg tggtttgctg atgaagtcct gtatggtgga ccactgatca
     5521 tcgtcgcgct ggtgatgatc ggttatgcaa tgcgcgagcg actgcctgaa tatagctttt
     5581 acggcgctct cctgatcaac ttgacggtca cactcgtttt cctgctggca gtcgttagtg
     5641 gaaagggcgt gatggatcgc gtcgtgttgg tgcgcttggt tcagctcaac gcgatcacct
     5701 tcgcggtcta tgcgttgccg tggttaagta cgcgacgatt gtggcaacag aaactcgatg
     5761 agcggcggca gacacgcgcc gacgacttgc tcgaactgca actcggattg gcgataggcc
     5821 tgaacctgtt cttaatcgtt ccggtggcgc tcgggctcat aatcgaaccg gaaccgcccg
     5881 gcattggaac aatcgccgcg ggaactttgc tcgggtggtt ggcgttcgca acgaccgcaa
     5941 ccgcagttgc atggttgact cgaatccgct cgcgcgatct ttccgccgtg ttattcgctg
     6001 gcggattaat ggccgtcagt tgtttgtttg cttttactgt tgctgatacc agcggatggt
     6061 tggcactgca cgtcttaacc gtctgtgtca cgttgtcggc atggttgatg ttggtcgctg
     6121 ctgatcggga tgcgcggtgg caacgtcagt gccgccaact cgcggcagtc agcggcagcc
     6181 ttgccgcatt tttgagtgtg cgaacagcca cggatttaga cgcgactggc tggtggtcag
     6241 ttggaccatt ggtgtcgctg agcatattgt tcgcggtttt aaactggcat acacttcgcc
     6301 gaagatacct ttacgttgcc ggcgtgttgt tcactttcgc agcctccatc tggtgcgttc
     6361 gaattctgcc agactccttc gaagaaggat ttgtcgccat caacgttgcc gccggttgcc
     6421 tggcaggcat cgttctgttg tggctggagc ttcgagcacg ccggttgacg gacactgaaa
     6481 caccacagtc tacggtcttg tcgtttcaca atctcagcgc catcggctgg ccgctgctac
     6541 tggcgtttct agtggcgatc agtcctggtg ttgcgaggtt caattcaccg gtagcgacta
     6601 tgccggggct gcactggata gcgatggccg caaccattgc cttgatgacg gccgttctct
     6661 gggacgaaca cgcaaagtat gcagttgcc
//
LOCUS       DASTEY010000487         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_225040, whole genome shotgun sequence.
ACCESSION   DASTEY010000487 DASTEY010000000
VERSION     DASTEY010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225040"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VFO72_08545"
     CDS             <1..1106
                     /locus_tag="VFO72_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479379.1"
                     /translation="SSEHLYDGRLFGEPLSAEAELTIEGVTFVAHTDIRRDVAPAVEI
                     LEVEPVPYVTTPATSQKPLEFKVRLQNHLTTPFRGLLRVRGPSLETGKEINLQPNQIE
                     ASNLVVRSLALTGLMQNSNVDITVDLPNPKDPIAKRVVPLTYANAIVVAGRKVGYIPS
                     YDRTLERALSSLAVDAKQLTVDDIAKADLSPFHTIIIDNRGYEAHQQLIGLNSRLLKF
                     VEDGGTLLVFYHRVNEWNPDERRNRPQLAPYPIIVGAERVTQEDALVTFLQPGHPLLR
                     FPNRITQADFADWIQERGLYFPREWDPRYATVLATSDKGEPLLRGGLLVAPYGRGNYI
                     YTSYVWYRQLRAGLPGGYRFFANLISYGRKKED"
     gene            complement(1210..1734)
                     /locus_tag="VFO72_08550"
     CDS             complement(1210..1734)
                     /locus_tag="VFO72_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479380.1"
                     /translation="MFRIKASYSDQLELRTTVERAREFFGELRNFADLMPGIEGIRKE
                     AGGIMRWMVRAEVPIIGAVHATFTVEKTEDSPERLEWSPARSEMKNYLRYAAAFEERG
                     HKVLIKIVQHVELRRQHAKDLHRLAILVGESAISAEMQKRVREMIKTFLERARVKLEG
                     EEPLEQEAMADSPA"
BASE COUNT          492 a          617 c          653 g          490 t
ORIGIN      
        1 catcttcgga acatctttac gacggccgtc tctttggcga accactcagc gccgaagcgg
       61 aactgacaat cgagggcgtc acgtttgttg cccacactga catccgcaga gacgtcgcgc
      121 cggccgtcga gattctcgag gtcgagccgg tgccttacgt cactacgcct gcaacatcac
      181 agaaaccact cgagttcaag gtgcgactac agaatcatct caccactccg tttcgtgggc
      241 tgttgcgcgt gcgtggtcca agcctggaaa ccggaaaaga gatcaatctg caaccgaacc
      301 agattgaagc gtcgaacctg gtcgtgcgca gtctggcttt gaccgggttg atgcaaaatt
      361 caaacgtcga catcaccgtc gatctgccga atccgaaaga cccgattgcg aaacgggttg
      421 tgccgttgac ctacgccaac gcgatcgtgg ttgccgggcg caaggttggt tacataccga
      481 gttacgacag gacgctcgaa cgcgcgttga gttcgctggc cgtcgatgcg aagcagttga
      541 cggttgacga cattgcgaaa gctgatttgt caccgtttca cacgatcatc atcgacaatc
      601 gtggttacga agctcatcag caactaatcg gactgaatag ccgtctgctg aagttcgtcg
      661 aagatggtgg aacgctgctc gtgttttatc accgggtgaa tgagtggaac cccgacgagc
      721 gacgtaatcg tccgcaactc gcgccgtacc cgatcatcgt tggtgccgag cgtgttacgc
      781 aggaggatgc gctcgttacg tttctacagc cggggcatcc gctgcttcgt tttcccaacc
      841 ggatcacgca agccgacttt gcagactgga tccaggaacg aggactctat tttccacgtg
      901 aatgggatcc gcgttacgcg accgtgttgg cgaccagcga caaaggcgag ccgctgcttc
      961 gtggtggttt gctcgtggcg ccgtacgggc gtggaaacta catctacacg agctacgtct
     1021 ggtaccggca actgcgcgcg ggactaccgg gcggatatcg cttctttgca aatctgatta
     1081 gttacggcag gaagaaagag gactaggttg catcaaacgc cgaaggcgtt cacgagtttc
     1141 agcccagggt ttcttcaacc ctgggaaacc agccggacaa ttagcataga acgctgaaag
     1201 cgttggcgat taggctggcg agtcggccat tgcttcctgt tcgagcggtt cctcgccttc
     1261 gagtttgacg cgtgcgcgtt cgagaaaggt cttgatcatc tcgcgcacgc gtttttgcat
     1321 ttcggcgctg atggcgctct cgccgacaag gatcgcgaga cggtgcaaat ctttggcgtg
     1381 ctggcgacgc agctcgacgt gctggacgat cttgatcagt actttgtggc cacgctcttc
     1441 aaaagccgcg gcgtagcgaa gatagttctt catttcggaa cgggccggag accactcgag
     1501 acgttccgga gagtcttcag tcttttcgac agtgaaggtg gcatgaaccg cgccgatgat
     1561 cggcacctcg gcacgcacca tccagcgcat gatgcctcct gcttccttgc ggatgccctc
     1621 gatgccgggc atcaggtccg caaaattacg gagctcgccg aaaaactcgc gggcacgttc
     1681 gacggtggtc cgcaattcca gttggtctga gtaagaagct ttgatacgaa acatttgttt
     1741 tccgttaacc gttctccgac atgttgatgc gcgagggcgg cagtttggtt ttctgcggca
     1801 ccattgctaa gttacacgag gaatatgcac tagtaaatat ccaagccaac aaatatcgac
     1861 gttgccagga aagaattatc tatatgcgtg ccccgcacac atgtccgcgc tgcgataaac
     1921 gtgggatgaa atcgtggtct gaactaaccg acgagcagcg ggaagtcgtg aaacggctgc
     1981 cgctttccgc cagctaccca ctcgaagaac gccaggcgac tcaccgctgg tgcacccgct
     2041 gctggtacga atccaccgag accgagacac acgcttaaag aatgcggttg tagctttcgc
     2101 ggatcgactc catctgttcg agaagggttt ggtgcaggtc tactgcggac gcgaagccca
     2161 tgcggatggc gacgtcgctg gcggtgacgt gattcggatc cggcagacgc gtcgagcggc
     2221 cgacgatgag gcgcaggttg tgatcgatcg ag
//
LOCUS       DASTEY010000488         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_867702, whole genome shotgun sequence.
ACCESSION   DASTEY010000488 DASTEY010000000
VERSION     DASTEY010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867702"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..569
                     /locus_tag="VFO72_08555"
     CDS             39..569
                     /locus_tag="VFO72_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479381.1"
                     /translation="MLSAFLGLASLAGALSGGTSKAATMDGGVNAHWKGMESFSLTNA
                     GPNVSRCGAFPENIELTFAGSGIDTEGGLFDVVASACTNTTTNEIFDLKATDTYVQSG
                     DQIFIEADSFVQIINPANCVATNSHAVMGRVAGGTGGHAGAKGHARFHFANNLTPCNG
                     QNAPAHVWFEGVVERD"
     gene            648..1217
                     /locus_tag="VFO72_08560"
     CDS             648..1217
                     /locus_tag="VFO72_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9479382.1"
                     /translation="MEKPGVQELTQLLAAWSDGEEQALEKLAPLVHAELYRLAKRYMS
                     RERPDHLLQTSALINEAYVRLIDWKSVRWQNRAHFFGVAAQIMRRILVDFARRRPRVD
                     RDLEAIRMPLDEAMTISSERDPDLLALDEALKSLATIDERKSRIVELRFFGGLSVDET
                     AEVMQMSSITVIREWNKAKAWLYRELSNN"
     gene            1222..>2523
                     /locus_tag="VFO72_08565"
     CDS             1222..>2523
                     /locus_tag="VFO72_08565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9479383.1"
                     /translation="MDHELWKQVDALLEQALEQPPEEREAFVVAASGDNPVLRDEVLS
                     LLKAQSQAAKFMERSAMNVAAQALAQNSDFTTVASLVGQELETYRIEKLLGAGGMGEV
                     YLARDLKLGRMVALKVLPWHFVVDSDRLGRFQREARALSSLNHPNLVTIYEIGEAKGL
                     HFIAMELVEGKTLSSLRDKFSLKELLSIVAQVAEALSAAHQSGIIHRDVKPDNVMVRP
                     DGYAKVLDFGLVKLTEVEPAPVDEARTQLGVAMGTLAYMSPEQAAGEPVDQRTDIWSL
                     GVVLYELATGRKPFAGETRQATINAILSTQPNQATTIAPGLPPDLDQILNKALEKDRE
                     LRYQTASDFRADLRRLLRQIDSSPDVSGEGSKSGRVQQFTEHRWLWPVVASVVILVIA
                     GLAWSAWLKAKADAPDWSRAAHLQLTSEHGTEFFPSLAPDGK"
BASE COUNT          591 a          711 c          704 g          517 t
ORIGIN      
        1 ggagctaaat tgaaatgcga actaccaaac ttacattcat gctgtcagca ttcttaggac
       61 tcgcctcact tgccggagct ctaagcggcg ggacttcgaa agccgcgacg atggacggtg
      121 gcgttaacgc acactggaaa ggcatggaga gctttagtct caccaacgcc ggaccaaacg
      181 tctctcgttg cggcgcattt ccggagaaca tcgagttgac attcgccggc tcgggcattg
      241 ataccgaagg cgggctcttt gacgtcgtcg cttcagcctg caccaatact accaccaatg
      301 aaattttcga tctgaaagca actgacacgt acgtgcagag cggcgatcag attttcatcg
      361 aagcagattc gttcgtacag ataattaatc ctgccaactg cgtcgcaaca aactcgcacg
      421 ccgtaatggg acgcgtcgca ggcggcacag gcggacacgc gggcgcgaag ggccatgcgc
      481 ggttccactt cgccaacaat ctaactccct gcaacggaca gaatgcgccg gcacacgtct
      541 ggtttgaagg agtcgtcgag cgcgactaat aacaactcgg aaaaggttag acggcctgga
      601 gcgcacagtt ccaggccgct tttttatgtg ataaattcag cgccccaatg gaaaaaccgg
      661 gcgttcagga acttacacaa cttttagcag cctggagcga cggcgaggaa caggcgctcg
      721 agaaacttgc gccactggtc cacgccgaac tataccgcct ggccaaacgc tacatgagtc
      781 gcgagcggcc ggaccatcta ctgcaaacct cagctctcat caacgaagca tacgttcgcc
      841 tcatcgactg gaagtcagtc cgctggcaga atcgcgcaca cttcttcggc gtggccgcgc
      901 aaatcatgcg tcgtatcctg gtcgactttg cgcgccgccg acctcgtgtc gatcgggatc
      961 tcgaggcgat ccgaatgccg cttgatgagg cgatgacgat cagcagcgaa cgcgatccgg
     1021 atctgttggc tcttgacgaa gcactgaaga gtctcgccac gattgacgag cgcaaaagtc
     1081 gcatcgttga gttgcgcttc tttggcggtt tgagcgtcga tgaaaccgcg gaagtcatgc
     1141 agatgtcttc catcactgtc atacgcgagt ggaataaggc caaagcctgg ctttatcgtg
     1201 aactcagcaa taactaacta aatggaccac gagctgtgga aacaagttga cgcactgctc
     1261 gagcaggcac tcgaacagcc gccggaagag agggaagcct ttgtcgtcgc tgcttccggg
     1321 gacaatcccg tattgcgcga tgaagtactg agtctgctga aggcgcagtc gcaggcggcg
     1381 aagtttatgg aacgttcagc aatgaacgtg gccgcccagg ctctcgcaca gaactccgat
     1441 ttcacaaccg ttgcttcact cgtaggccaa gaactcgaaa cctatcggat cgaaaaactg
     1501 ttgggcgccg gtggcatggg cgaagtctac ctcgctcgcg accttaaact cgggcgaatg
     1561 gtcgcgctta aagtgctgcc gtggcacttc gtcgttgata gcgaccgact aggccgtttc
     1621 caacgtgaag cgcgggcact ttcttcgttg aaccacccga acctggtgac aatctacgag
     1681 attggcgaag ctaaaggtct ccacttcatc gccatggaac tggtcgaggg caagacactc
     1741 tcctcactac gtgacaaatt tagtctaaag gaactgttgt cgatcgttgc tcaggttgcg
     1801 gaagcgctga gcgccgctca tcaatccggc atcattcatc gcgacgtaaa gcccgacaat
     1861 gtgatggtcc ggcctgatgg atatgcgaag gtgctggatt tcggtctggt aaaactcacg
     1921 gaagttgagc ctgcgccggt agacgaggcg cgcacacaac ttggtgtggc gatgggaacg
     1981 ttggcctaca tgtcgccgga acaagccgcg ggagagccgg tggaccaacg gacagatatt
     2041 tggagtcttg gcgtagtgct gtacgaactc gccaccgggc gcaaaccttt cgccggtgag
     2101 acgcgacaag ccaccatcaa cgcgatcctt tcaacacaac caaaccaggc gactacgatc
     2161 gcgccgggcc ttccacccga tctggatcag atcctcaaca aagcactcga gaaagatcgg
     2221 gaactgcgct atcaaaccgc ttccgacttt cgtgcagacc ttcgtcgttt gctgcgtcag
     2281 atcgattcgt ctcccgatgt ttcaggcgag ggatcgaagt ccggtcgcgt tcagcagttt
     2341 accgagcatc gctggctttg gcccgtcgtc gcgtcggtcg tgatcttggt gatagcgggg
     2401 ctcgcgtggt ctgcatggtt aaaggccaaa gccgacgcgc ccgattggtc gcgcgcagca
     2461 catttgcaac ttaccagcga acacggcacc gagttctttc cgagtcttgc acccgatggc
     2521 aaa
//
LOCUS       DASTEY010000489         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1494241, whole genome shotgun sequence.
ACCESSION   DASTEY010000489 DASTEY010000000
VERSION     DASTEY010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494241"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFO72_08570"
     CDS             complement(<1..577)
                     /locus_tag="VFO72_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HET9479384.1"
                     /translation="MSTAEDAASTAPARTRTFFGHPIGLSTLFFTEMWERFSFYGLRP
                     LLVLFMSAALADGGFGIERSQASAIVGIYAGSVYLSSLPGGWVADRLLGLRRAIMLGA
                     VLISLGHISIGMSSFAHIKLPFFLGLVLIVLGTGLLKPNISAIVGDLYPEGGARRDAG
                     FSIFYMGINTGAIFGQLATGYLGEKIGWHWGF"
     gene            complement(659..1258)
                     /locus_tag="VFO72_08575"
     CDS             complement(659..1258)
                     /locus_tag="VFO72_08575"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9479385.1"
                     /translation="MKFPRKLPLFRSLKIATFAILSFFIFTNVTGFLFSNVMAPVAVA
                     NPTVSRKIPADLPTSGDENIDRLIFEAADREGLDPRFVHAVIWQESKYDVDARSHAGA
                     EGLMQLMPATAERFGCQDRTDPVSNIQAGTKYLRWLLKRFDGNVELALAGYNAGEGAV
                     DKYNGIPPYNETRNYVKIISKRYGKTYHPVVTPVEVEAD"
     regulatory      1516..1591
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1788..3641
                     /locus_tag="VFO72_08580"
     CDS             1788..3641
                     /locus_tag="VFO72_08580"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET9479386.1"
                     /translation="MQPFRELLESDRIHVVDGAMGTMLYAKGVYINRCYDEMNLSNPD
                     LVREIHSEYIRAGADIIETNTFGATGHKLQQYGLEGSLHEINATAARIARDAAGDRAY
                     VAGAIGPLGLRIEPYGPTSFEEAKDMFKAQATALLEGGVDLFCLETFSDVSEIRQAIA
                     AVREVCDLPIIAQMTIMTDGNTLFGTTPELFTERLDEWGADVIGLNCGVGPAIILNAL
                     EKMREVTKKKLSAQPNAGLPRDVQGRQFYMCSPEYMSKFAKRFIQSGAKFIGGCCGTT
                     PAHIKLISDSVRAASPRAQGAVFSGAPAQVVEITAPDVRVVPPEERSAWGRKIARGEF
                     VTSVEVLPPKGVDAKKTLDSIRLLKDAGVDGVNIPDGARAQSRMSALATAVLVEQQIG
                     IESVLHYCCRDRNLLGMMSDLLGAAALGLRNILVITGDPPKMGPYPDATAVFDIDSIG
                     LTNMVNKLNHGLDLGNNPIGEPTAFCIGVGVNPGAINLDEEIKRFEWKVEAGAQYAIT
                     QPVFDTEQLRNFLKRIQHVRIPIVAGIWPLVSYRNAEFLHNEVPGVRVTPSIMDRMRT
                     ASAISKEAGREEGLKIARESLLEVRDEIQGVQVSAPFGNVKYALEVFDVLQ"
BASE COUNT          952 a         1061 c         1122 g          816 t
ORIGIN      
        1 cgaaacccca gtgccagccg atcttctctc ccaggtaacc cgtggcaagc tggccgaaga
       61 tcgcgccggt gttaatcccc atgtaaaaga tcgaaaaacc tgcgtcacgg cgtgcgccgc
      121 cttcgggata caagtctccg acgatggcgg agatgtttgg tttcagtaga ccagtaccaa
      181 ggacaattag caccaggccc aggaagaacg gcagttttat gtgtgcgaac gacgacatgc
      241 cgatcgatat gtggcccaat gagatcagga ccgcgcccag catgatggcc cgtcgcaggc
      301 ccaacaaacg atcggcaacc cacccgccgg gcaaagacga aaggtagaca cttcccgcat
      361 atataccgac gatggctgac gcctgactcc gctcaattcc gaaaccgccg tcggccaatg
      421 cagccgacat gaaaagcacg agcagtgggc gtaggccata aaacgaaaac cgctcccaca
      481 tctctgtgaa gaagagcgtt gaaagaccga ttgggtgtcc aaaaaaagtg cgagtcctgg
      541 cgggtgcagt ggaagctgca tcttctgctg tgctcatgcg agccattcaa gcatctgtgt
      601 tatccgtggt caaggatcag gaccacggat aacacggatg ttggaaatag gaggagggct
      661 agtctgcttc gacttcgacg ggcgtcacga ccggatggta ggttttgccg taacgcttgg
      721 aaatgatctt tacgtagttg cgcgtttcgt tgtacggcgg aatgccgttg tacttatcaa
      781 ccgcgccctc acccgcgttg taacccgcga gcgcaagctc aacgttgccg tcgaatcgct
      841 tcaacaacca gcgcaggtac tttgttcccg cttgtatgtt cgacacgggg tctgttcgat
      901 cttggcagcc aaatcgttcc gcagtcgcgg gcatcaactg catcaaccct tcggctcccg
      961 cgtgagaccg cgcgtctaca tcgtacttcg actcctgcca gatgaccgcg tgaacgaatc
     1021 gtggatccaa gccctcgcgg tcagcggctt caaaaataag ccggtcgatg ttctcatcac
     1081 ccgacgtcgg tagatcagca gggatcttgc gcgaaacggt gggattcgcg acagccacag
     1141 gggccatcac gttactgaac agaaagccgg ttacgttagt aaaaataaag aatgaaagaa
     1201 tggcgaatgt cgcgatcttc agcgaacgaa ataggggcaa cttgcgggga aacttcattc
     1261 agaacaatct ccagggttaa tttgtacata agggctcacc tctttccggg gccgcgagtc
     1321 tgatggtcca ttagcgacca ttagttgctt ttttcttaca caatttttgc tcgtaagttc
     1381 ttgaaaatca tgtacttaaa cggttcgacc agatcggtcg atctgagaga acaattcagc
     1441 ctgacagaaa aaaccagaca ctctggtgct gggtctgcag cttgacagcc gccgcgggtg
     1501 aggcgtataa gcagccatga caaacgtggt gagttaaggc gacttgcggc cacgtaaaac
     1561 aaccagctaa acagctcggg cgccttagat aaagcctttg acaacgcttt tgacaaagcc
     1621 gctgaaaaag gtagtcaggc ccgaaaaact tcaaatagcg atttgaagtc ccgttgttta
     1681 gcgcgggact tttttcattt ttgatttttg attttgattt ttgattgtca gacccaagta
     1741 atggctttca aatcggcaat caaaaatcaa aaatcggcaa taccaacatg cagccatttc
     1801 gcgaacttct cgagtcagac cgaatccacg tcgttgacgg tgcgatgggt acgatgctct
     1861 acgccaaggg cgtctacatc aatcgctgct acgacgaaat gaatcttagt aatccggatc
     1921 tggtgcgcga gatccacagt gagtacatcc gcgccggcgc cgacattatc gagacaaaca
     1981 cgttcggcgc caccggccac aaactccagc agtacggact cgaaggcagt ctgcacgaga
     2041 tcaacgccac cgccgcaagg atcgcacgcg acgccgcggg cgatcgcgct tacgttgccg
     2101 gcgcgatagg accactggga ctgcgcatcg agccgtatgg tccaacttca ttcgaggaag
     2161 ccaaggacat gttcaaggca caggcgacgg cgttgcttga aggcggcgtc gatctctttt
     2221 gcctcgaaac tttctccgac gtctccgaga ttcgccaggc catcgcagca gtgcgcgagg
     2281 tttgcgatct gccgatcatc gcgcagatga cgatcatgac cgacggcaac acgttgtttg
     2341 gcactacgcc cgaacttttc accgagcgtc ttgacgagtg gggcgctgac gttatcggtc
     2401 taaactgcgg cgtcggacca gccatcattc tcaacgctct ggaaaagatg cgtgaagtca
     2461 cgaagaagaa gctgtcggca caaccaaacg cgggactgcc gcgcgacgtg caggggcgac
     2521 agttttacat gtgctcgccc gagtacatgt cgaagtttgc gaagcgattc atccagtcgg
     2581 gtgcgaagtt catcggcgga tgctgcggca cgacgccggc acatatcaag ttgatctccg
     2641 attctgtgcg tgcagcgtct ccgcgtgcgc agggtgcagt gttcagtgga gcgccggcgc
     2701 aggtggtaga gatcaccgca ccagacgtga gggttgttcc acctgaagaa cgatccgcgt
     2761 ggggacgaaa gatcgcgcgc ggcgagtttg tgacgtcggt cgaagtgctg ccgccgaagg
     2821 gagtcgacgc gaagaagacg ctcgactcga ttcgcttgct gaaagatgcc ggggttgacg
     2881 gcgtgaacat acctgacggc gcgcgcgcgc agtcgcgcat gagcgcgctg gccacggccg
     2941 tgttggtcga gcagcagatc gggatcgagt ctgtgttgca ctactgctgt cgcgatcgaa
     3001 atctgctggg gatgatgtcg gacctgctcg gggccgcggc gctcgggctc cgcaacatcc
     3061 tcgtgatcac gggcgatccg ccgaagatgg gtccatatcc ggacgcgacc gcagtttttg
     3121 atatcgactc gatcggtttg accaacatgg tcaacaagct gaaccacggt ctcgatctcg
     3181 gaaacaaccc catcggcgag ccgaccgcgt tctgcatcgg tgtcggcgtg aatccgggcg
     3241 cgatcaatct cgacgaagag atcaagcggt tcgagtggaa agtggaagcc ggagcacaat
     3301 acgcgatcac gcagcctgtc tttgataccg agcagttgcg taattttctc aaacggatac
     3361 agcacgtgcg tattccgatc gtggccggca tttggccgct ggtctcgtat cgcaacgcgg
     3421 agtttctgca caatgaagta ccgggcgtac gcgtgacgcc gtcaatcatg gaccgcatgc
     3481 gtacggcctc ggcaatcagc aaagaagccg gacgcgaaga gggtttgaag atcgcccgcg
     3541 agtcgctgct ggaagtgaga gacgagattc agggcgtaca agtgtctgca ccatttggca
     3601 acgtgaagta cgcgttggaa gttttcgacg ttctacaata agagacgatt tcaggcatcc
     3661 agcttcacga ttgttggccc agtttagatg atgatgaact ccacggagca gccggcaatc
     3721 tgagagttta catcaagcac agacctccct cagaaaaccg agcgtggtgg ttttcccaaa
     3781 cagttctcgc gttaataagc agaaacaagc aaacgcacgc gaagttattg ggccagcgcg
     3841 cctaactggg gcgtgaaaca gcggggcggc ttcgtttgag ctaccacgta caccaaggaa
     3901 aaaagggagg attcatcatg tccactggag atttccactg cttctgcgag a
//
LOCUS       DASTEY010000490         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1197472, whole genome shotgun sequence.
ACCESSION   DASTEY010000490 DASTEY010000000
VERSION     DASTEY010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197472"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFO72_08585"
     CDS             <1..244
                     /locus_tag="VFO72_08585"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET9479387.1"
                     /translation="RDTAVASRANKPIGDRMIMNAAFLIERDREAEFDQKVKEIAAKY
                     EGKLSFKYTGPWPPYNFVHIRLKLERGEGATEAVAS"
     gene            248..577
                     /locus_tag="VFO72_08590"
     CDS             248..577
                     /locus_tag="VFO72_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HET9479388.1"
                     /translation="MFLIDDLLLAPVNGFKFILGQIQKMADQELNDDSVIKDQLLELQ
                     MRYELEEISDEEFKAREAELFARLRAIKMRQLDLLHEATAHTADSSSFVIEVGGDEGE
                     LGEPRKR"
     gene            574..1557
                     /locus_tag="VFO72_08595"
     CDS             574..1557
                     /locus_tag="VFO72_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET9479389.1"
                     /translation="MSRYLFFGGKGGVGKTTAASATALFLLNKLKAKSRTRKPGTAGV
                     SPAPSILLFSTDPAHSLSDSLGIKIGNRLVKVAERRGAKLIGYEMDATLALERFRAAH
                     GKVLAEIAERGTLLDEEDINELLNLSLPGLDEVMSLFELSELDREGRYTHIVVDTAPS
                     GHTSRLLRLPEVFTRMVHALDRMGEKHRYMVEHFARRKVAADEVDLFLRDLTARIGAV
                     RNMLYNPEETSFTLVTIPEAMSVRETQRYLKLLREQNVPVTDLIVNRVEQEHGDCVYC
                     RARVATQRPWLKEIEKSFGELKLHYVPLFAKEVGGVAQLEKIGKQIWTKTR"
     gene            1539..>2413
                     /locus_tag="VFO72_08600"
     CDS             1539..>2413
                     /locus_tag="VFO72_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HET9479390.1"
                     /translation="MDKNEIRKFVIFGGKGGVGKTTAAAAYALALAEADPKRKLLVFS
                     SDPAHSLSDSFGEEIGELKKGVAGYKNLDAMEIDPGKWFDELKQRYRSWTDDLFTSLS
                     GRSGMEIQFDREAMRELVELTPPGIDEIAALGTISELLGQDQKDDRYDSIILDTAPTG
                     HLIRFLELPEVALSWIRTFMKLLLKYQNVVRATEVAEELVALSKSIKRVLALLTDQEL
                     CEFVGVAIPERMSLEETIDMAKSLERLKVPLRKLLINGVVSEEAARQCRFCRTRRKGQ
                     LEVANEFHRKFRRRS"
BASE COUNT          553 a          590 c          753 g          517 t
ORIGIN      
        1 gcgcgatacg gccgttgcgt cgcgtgcaaa caaaccgatt ggtgatcgca tgatcatgaa
       61 cgccgcgttt ttgatcgagc gcgaccggga agctgagttt gaccagaagg tcaaagagat
      121 cgcggcaaag tacgaaggca agctgtcgtt caagtacacg ggaccgtggc cgccgtacaa
      181 cttcgtgcac atccggctta aactcgaacg cggggaaggc gccacggaag ctgtggccag
      241 ctaagcaatg tttctcatcg acgacctact gctcgcgccg gttaatggtt tcaagttcat
      301 tctcggccag atccagaaga tggccgatca ggagctcaac gacgattctg tgatcaagga
      361 ccagttgctt gagttgcaga tgcgttatga gctggaagag atctcggacg aagagttcaa
      421 ggcccgcgaa gcggaactgt ttgctcgtct gcgtgcgata aagatgcgcc agctcgatct
      481 gttgcacgaa gccacggccc atacagccga ctcgtcgagt tttgtgatcg aagttggcgg
      541 tgacgaaggc gaacttggcg aaccgcgcaa gaggtgagtc gatatctttt cttcggtggt
      601 aagggcgggg ttggaaaaac taccgctgcg tctgctaccg ccctctttct gctcaacaaa
      661 ctcaaagcaa aatctcggac ccgtaaacct gggacggcag gcgtctcgcc tgcaccgtcc
      721 atcctgctgt tctccaccga cccggcgcac tctctctcag atagtctcgg cataaagatc
      781 ggcaaccgcc tggtcaaagt tgcggaacgc cgtggcgcga aactgatcgg ctatgagatg
      841 gatgcgacgc tcgcgctcga gcggtttcgt gcggcccatg gaaaagtgtt ggcggagatc
      901 gcggaacgcg gcacgttgtt ggacgaagaa gacatcaacg agctcttgaa tctgtcgttg
      961 cctggtttgg atgaagtgat gtcgctgttt gagctgagcg aactggaccg cgaagggcga
     1021 tatacgcaca ttgtcgtcga tacggcgccg tctggtcaca cgtcgcggct cttgcgtttg
     1081 cctgaggtct tcacgcgcat ggtccatgcg ctggaccgaa tgggtgagaa gcatcgttac
     1141 atggtggagc actttgcgcg ccgcaaagtt gctgcggatg aggttgatct gttcctgcgc
     1201 gacttgacgg caagaatcgg ggcagtgcga aacatgcttt acaacccgga ggagacgagt
     1261 tttacgctgg ttactatccc ggaagcgatg agcgtgcgtg agacgcagcg atacctgaag
     1321 ctattgcggg agcagaacgt gccggtaacg gatctgatcg ttaatcgggt cgagcaggaa
     1381 cacggggact gtgtgtattg ccgggcgcgc gttgcgacgc agcggccgtg gttgaaggag
     1441 attgagaagt cgtttggtga gcttaagttg cattacgtgc cgctgtttgc gaaggaggtt
     1501 ggcggtgttg ctcagttaga gaagattgga aagcagattt ggacaaaaac gagataagaa
     1561 agtttgtgat ctttggcggg aagggcggtg ttgggaagac aaccgctgcc gcagcgtacg
     1621 cgctggcact cgccgaagcc gatccgaagc gcaagctgct ggtcttctcc agcgaccccg
     1681 cgcattcctt gtccgacagt tttggcgagg agatcggtga acttaaaaaa ggcgtcgccg
     1741 gatacaagaa tctcgatgcg atggagatcg atcccggtaa atggttcgac gagttgaaac
     1801 agcgctatcg ctcctggacc gacgatctct tcacgtcgtt gtcaggccgt tcgggaatgg
     1861 agatccagtt tgaccgcgaa gcgatgcgcg aactcgtcga gttaacaccc cccggaatcg
     1921 acgaaattgc agcgctcggc acgatcagcg aactgctggg ccaggatcag aaagatgacc
     1981 gatacgactc gataattctc gataccgctc cgacaggaca cctgatccgt tttttggaac
     2041 tgccggaagt ggcgctctcg tggatcagaa cgttcatgaa gttgcttttg aaataccaga
     2101 acgtggtgcg tgcgaccgaa gtcgccgaag aactcgtcgc gttgtcgaag agtatcaaac
     2161 gggtactcgc tttactaacg gatcaggagt tgtgtgagtt tgtgggcgtg gcaattcccg
     2221 aacggatgag tctggaagaa acgatcgata tggcaaagtc gttggagaga ctaaaagtgc
     2281 cgctgcgaaa gttgttgatc aatggagtcg tttcagaaga ggctgccagg cagtgccgct
     2341 tctgcaggac gcgccgcaaa gggcagctcg aggtggcgaa tgagtttcac cggaaattcc
     2401 ggcgtcgctc aat
//
LOCUS       DASTEY010000491         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_966645, whole genome shotgun sequence.
ACCESSION   DASTEY010000491 DASTEY010000000
VERSION     DASTEY010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966645"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFO72_08605"
     CDS             complement(<1..833)
                     /locus_tag="VFO72_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9479391.1"
                     /translation="MNLFDAIFAQNPSSPAICFGRRVITYSELRAETMRIAGIIHHLG
                     AGSGDRVALLLHDSPKFIEAFIATCSLGAISVPINMALRADEQCSILHNSGASLAFME
                     SDTCNALLTHAPEKLRSLKNIVSVKPNDEPERSDEVEDRVSISERQLRNPPLMIYDLI
                     DLLDQPYADQQPEFPQPQRDEPAFILYTSGSTGEPKGAVHCQSDIFYTNETFCHEVLQ
                     LTPDDRLFSSSRLPFAYGLGNSFSFPLLNGATTILCREKPSRDLIARIFREQRPTIFF
                     GV"
     gene            904..>2371
                     /locus_tag="VFO72_08610"
     CDS             904..>2371
                     /locus_tag="VFO72_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479392.1"
                     /translation="MEFLFLLMVVLFVLLVLLAIMTVAGHAMWLMVAAVLRFLFSDGT
                     SPNYRRQSTRIFEPSLKPVDDLDITERQIVTFYVDGKIDERTYNQLIRRIRAERASLT
                     PAVKVPASTPVPPPKPVEVPRPAEVHSVVTAALVASDDTIVIKPVPTFIIDDEPPSSS
                     PRPPRYQRPTPPPRPPRRPFSEVLNSFMEESNIRWGEIIGGLLIIGCSTALVVSLWAQ
                     ISQIPVLKFLIFTTVTAILFGIGLYTEHRWKLPTTSRGILTIATLLVPLNFLAIAAVS
                     ASNTSGALVIGSELLAPAIFLCLVYFAGRVITPGCAHILSAGVLGSSVGQLLVRHFAG
                     IDTSPALLVLLGAFPVACYVATVGLALRIVLDDREIDASETTTVFTILGTMSFAALLP
                     FALLLYKSGPLGMSMMYLAPLVTLWGLPMLATGTILWRRINNKELVASRTTGTALGIL
                     GVMIVLAGMMLAWPNPASIVPAALLNFAIFTALAVALEL"
BASE COUNT          493 a          672 c          662 g          544 t
ORIGIN      
        1 acgccaaaaa agatcgtcgg acgttgctcg cggaagatcc tggcgatcag atcccgtgaa
       61 ggcttctcac gacagagaat cgttgttgct ccgttcagga gtggaaacga gaaactgttt
      121 ccgagaccat aagcgaacgg caatcgcgac gatgagaaga gacgatcatc gggcgtgagc
      181 tgcaaaactt cgtgacagaa agtttcgttt gtgtagaaga tgtctgattg gcagtggacc
      241 gcgcctttgg gttcaccggt gctgcccgat gtgtagagta tgaaagcagg ttcgtctcgt
      301 tgcggctgcg gaaactctgg ttgctggtcc gcgtaaggtt gatccaacaa atcgatcagg
      361 tcatagatca tgagtggcgg atttcgcagt tgcctctcgc tgatagaaac acggtcttcg
      421 acttcgtctg acctttccgg ctcgtcgttt ggcttaacgg aaacaatatt cttcagagat
      481 cgcagctttt ccggcgcatg tgtcagcaat gcattgcagg tgtcagattc cataaacgcc
      541 aggctggccc cggaattgtg aaggatcgaa cactgttcgt cggcacgcag ggccatgttg
      601 atagggaccg agatcgcacc tagcgaacac gtggcgataa acgcctcaat aaatttcggt
      661 gagtcgtgga ggagcagcgc cacacgatcc ccggagccgg cgccgagatg gtggataatc
      721 ccggctatcc tcatcgtctc ggcacgcaac tcactatagg tgatgacgcg ccggccaaaa
      781 cagatcgcgg gcgatgacgg gttttgtgcg aagatagcgt cgaagaggtt catagtcctg
      841 gccggggaga aaaatcttat agcacagcct aactagggtc caccgcgaac ggagcaaaga
      901 atcatggaat tcctgttcct gttaatggtc gtcttatttg tcctcctggt tctcctggcg
      961 atcatgaccg ttgccggcca cgccatgtgg ctgatggtcg ctgcggtcct gcggttctta
     1021 ttttcagatg gcaccagccc aaactaccgc cgccagtcaa ctcgaatatt cgagccgagc
     1081 cttaaaccag tcgacgatct cgatatcacc gaacgccaaa tcgtcacttt ctacgtcgac
     1141 ggcaaaatag atgagcggac atataaccag ttgatcaggc gcatacgcgc tgagcgagcc
     1201 agcctcaccc ctgcggtgaa ggtgccggca agtacgccag tcccaccgcc caaacccgta
     1261 gaggttccac gacctgcaga agtgcactca gtcgtaactg ctgcgcttgt cgcaagtgac
     1321 gacacgatcg taatcaaacc ggttccaacg ttcatcattg atgatgaacc gccatcgtcg
     1381 tcgccgcggc cgccgagata ccaacgaccc actccaccac cgcgcccgcc gcgacgtccc
     1441 ttttcagaag tgctcaactc cttcatggaa gagagcaaca ttcgttgggg cgagattatc
     1501 ggcggtctgc tgatcatcgg ctgttcgact gcgctcgtag tcagcttgtg ggcacagatc
     1561 tctcagattc cggttctgaa gtttctcatc ttcacgactg ttaccgcgat cctcttcggg
     1621 attggtctgt acaccgagca tcgttggaaa ctgccgacga cgagccgcgg cattctaacg
     1681 atcgccacgc tgctcgtgcc gctgaacttt ctcgcgatcg cggcagtgtc ggccagcaac
     1741 acgtcaggcg cgctggtgat aggcagcgaa ttgctggcgc cggcgatctt tctctgcctg
     1801 gtttattttg cgggccgcgt tatcacacct ggttgcgcgc atatcctgtc agcaggcgtg
     1861 cttggatctt ctgtcgggca actgctcgtg cgtcacttcg cggggatcga tacttctccc
     1921 gcgttactgg tcctgctcgg cgcgtttccc gttgcgtgtt acgtcgctac ggttgggctc
     1981 gctctgcgca tagtgctcga cgatcgcgag attgatgcaa gcgaaaccac cacggtcttc
     2041 accattctcg gaacgatgag cttcgcggca ctgctgccgt tcgccctgct gctttacaag
     2101 tcggggccgc tgggcatgtc gatgatgtac cttgcgccgc tggtaacact gtggggcctg
     2161 cccatgctcg ctacgggaac aatcctgtgg cgcagaatta acaacaaaga actcgttgct
     2221 tcgaggacca ccggcaccgc gctcggaatc ctcggcgtga tgattgtgct ggcaggaatg
     2281 atgctggctt ggcccaatcc tgccagcatc gttccggcgg cactgcttaa ctttgcgatc
     2341 ttcacggcgc tcgccgttgc gcttgagtta c
//
LOCUS       DASTEY010000492         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1494306, whole genome shotgun sequence.
ACCESSION   DASTEY010000492 DASTEY010000000
VERSION     DASTEY010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494306"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFO72_08615"
     CDS             complement(<1..230)
                     /locus_tag="VFO72_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9479393.1"
                     /translation="MTFESYESRLDAALRTFIHTNLIGVLFTDLAGQIISADQSFLNL
                     VGYTGDDLPPNLRDLISPEHHRLDEEAFEKLMA"
     gene            complement(343..2598)
                     /locus_tag="VFO72_08620"
     CDS             complement(343..2598)
                     /locus_tag="VFO72_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET9479394.1"
                     /translation="MKRRVAIIPIILWLAVSAFAQTELLTVDSIFTYRSRFLGPVRWQ
                     ENGRGYLALEPSPTKRNFVDIVLYDAATGQRSVKVSAEQLTPTGAKAPLLVEEFAITS
                     DEQKLLIFTNSQRVWRSNTRGDYWVLDLKANALRKLGGPDAKPSTLMFAKFSPDGQRI
                     GYVRENNIYVENLFGDNKITKLTTDGSRYIVNGTFDWVYEEELFCRDGFRWSPDSKQI
                     AYWQLNSEGVKEMQMIDNTSNLYPVIISFPYPKAGETNSAARVGVINASGGPTRWFDV
                     PGDPRNNYLPRMEWASADEVVIQQLNRRQDTNLVMMGDVNSGKVRPILTEKDEAWVDI
                     AWGDIDWNKTGIARGDVEWVENGKRFLWASERDGWRHIYSVSRDGRDVKLITPGNYDV
                     ITVEQVDAKNGWVYFLASPQNATQRYLYRVRVDGSGKEERLTPANAPGVHSYNISPNG
                     DFAIHVHSTFNTVPVTDVVRLPQHEVARTLIDNRAVQERLGKLKPAATEFFKVDIGDG
                     VQLDGWMIKPPDFDPQKKYPVLFYVYGEPWGQTVLDRWGGRDRMWHTMLAQQGYLVMS
                     VDNRGTPAPRGRAWRKIVYRRMGIVNSGDQAAAARAIAKWPFVDSARIGIWGWSGGGS
                     STLNALFRYPDVYSTGVSVAPVPDLRYYDTIYQERYGGLPQDHPEEWKQSSPITFAGQ
                     LKGNLMIIHGTGDDNVHYQGTEALINALIAANKQFAMMSYPNRSHSISEGPGTQRHLF
                     GVMTKYIKERL"
     gene            complement(2655..>4394)
                     /locus_tag="VFO72_08625"
     CDS             complement(2655..>4394)
                     /locus_tag="VFO72_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479395.1"
                     /translation="KETVTEKHPYGPSGSGVFACRYSPLLDENNNNSGGIAVVSDISA
                     RRQAEHAANTAYRQLAFHVESSPLAVVEWDSDFRVSRWSESAERLFGWKAEEVIGKHV
                     NEWQFVFADDVDAVALVTNRQREGVEVQGVQRNRNYTRDGSVLFCEWYNSVLHDDSGQ
                     MVSVLSLVLDVTAREKAEEERAALLIRERDARQHAEEADRLKDEFLATLSHELRTPLT
                     SILGWASMIRNGEVEGSSATRAIETIERNARSQARLIDDLLDVSRIITGNLRLDLHPL
                     NLAPIVEAAIDALRPTADVKGIQLRTESLGSDCLVKGDPNRLRQVIWNLLSNAIKFTQ
                     RGGVVVIDLRSVGSTVRLTVADTGEGISAEFLPYVFDRFRQAEGSISRKQGGLGLGLA
                     VVRHLVELHGGSIYAESAGLGQGSLFTVELPLAQERRDPARAEERRREVERRRSRTGA
                     IRLDGVHVLLVEDDDDSRKLLGTMLKRYGAKVTATKSAAEALEAFSGDLPDILISDIG
                     MPDEDGYEFIHKLRALPPEKGGQIPAIALTGYASRKDRERALAAGYQQHMAKPIEQAE
                     MISAIAALILRGD"
BASE COUNT          909 a         1294 c         1186 g         1005 t
ORIGIN      
        1 gccatcagtt tttcaaatgc ctcttcgtca agacggtgat gttcggggga aatcagatcg
       61 cgcagatttg gcggcaaatc gtcaccggtg tagcccacaa ggtttaaaaa actctggtcc
      121 gcggagatga tctgtcccgc cagatcggta aacaacacgc caataagatt cgtgtgaatg
      181 aatgtgcgca gggctgcgtc gagccggctc tcgtagcttt cgaaggtcat gattgaattt
      241 tccgaggaag gtgagtaggc aagaaggttg gccgtcaacc cgaaccacag attagcacag
      301 aagacacaga tttccccaga aaaatctgtg tccgcctgtg gcttacagtc gttctttgat
      361 gtacttcgtc atgacaccaa acaggtgccg ctgcgttccc ggcccttccg agatcgaatg
      421 tgaacgattc ggatacgaca tcatcgcaaa ctgcttgttg gccgcgatca gcgcattaat
      481 cagcgcttca gtgccctggt aatgcacgtt gtcgtcgccc gtgccgtgaa tgatcatgag
      541 gttgcccttc aactgcccgg caaatgtgat tggcgagctc tgtttccact cttcaggatg
      601 gtcctgcggc aggccgccgt aacgttcctg ataaatcgtg tcgtagtaac gaaggtccgg
      661 caccggcgcg accgagacgc cggtcgagta gacgtcagga taccgaaata gcgcgttcag
      721 tgttgacgag ccaccaccgc tccaacccca gattccgatc cgagccgaat cgacaaacgg
      781 ccacttcgca atcgcgcgtg cagccgccgc ctgatcgccg gaattgacga tgcccatcct
      841 gcgatagaca atcttgcgcc acgcgcgccc gcgcggcgcc ggtgtgccac ggttgtcgac
      901 gctcatcacc aaataaccct gctgcgccag catcgtgtgc cacatccggt cgcggccgcc
      961 ccaccgatcg agtacagtct gtccccaagg ttcgccgtaa acataaaaca gcaccggata
     1021 cttcttctgc ggatcgaagt cgggtggctt gatcatccag ccgtcgagtt gcacgccgtc
     1081 gccaatgtcg actttgaaaa actccgtcgc ggccggtttc aacttaccca gccgctcctg
     1141 gaccgcgcgg ttgtcaatca acgttcgcgc aacttcgtgc tgcggcagac gcacaacgtc
     1201 agtcacggga acagtgttga aggttgaatg aacgtgaatc gcaaagtcgc cgtttgggga
     1261 gatgttgtaa ctatgtacgc ccggcgcgtt tgctggtgtc agacgttctt ctttgccgct
     1321 gccgtcgaca cgtacgcgat acaggtaacg ttgtgttgca ttctgtggcg aagcaagaaa
     1381 gtagacccag ccgtttttcg catcgacctg ctcgacagtg ataacgtcgt aattgccagg
     1441 cgtgatcagt ttcacatcgc ggccgtcgcg agacactgaa tatatgtgtc gccagccgtc
     1501 gcgttcgctg gcccagagaa aacgtttgcc gttctcaacc cactcgacat caccgcgcgc
     1561 gatgccggtc ttgttccagt cgatgtcgcc ccacgcaatg tcgacccacg cttcgtcttt
     1621 ctcagtcagg atgggacgca ctttgccgga attcacatcg cccatcatca cgaggttggt
     1681 gtcctgccgc cggttgagtt gttgaatcac gacttcgtcc gcgctcgccc attccatgcg
     1741 cggcaagtag ttgtttcgcg gatcgcctgg aacatcgaac cagcgagttg ggccaccgct
     1801 ggcattgatg acgcccacac gtgcggcaga atttgtttcg cctgctttgg ggtacggaaa
     1861 agagatgatt acgggataca gattgctcgt gttgtcgatc atctgcatct ccttgacgcc
     1921 ttcagagttg agctgccagt aagcaatctg tttgctgtcc gggctccagc gaaaaccgtc
     1981 gcggcagaac aactcctctt cgtacaccca atcgaacgtg ccgttcacga tgtagcggga
     2041 gccgtcggta gtgagtttcg tgattttgtt atcgccgaac aggttttcga cgtagatgtt
     2101 gttttcacgg acgtaaccga tgcgttggcc gtcgggcgag aacttcgcaa acatcagcgt
     2161 tgatggtttg gcgtcagggc caccaagttt gcgcagagcg ttggctttga gatcgagcac
     2221 ccagtaatcg ccgcgcgtat tgctgcgcca gactctttgc gagttggtga agatgagtag
     2281 tttctgttcg tcgctggtaa tggcgaactc ctcgaccagg agtggcgcct ttgcgcctgt
     2341 gggcgtgagt tgttccgctg atactttgac ggagcgctgt cccgtcgcgg catcgtaaag
     2401 aacaatgtcc acaaaattgc gtttggttgg agacggctcc agcgccagat agcccctgcc
     2461 gttttcctgc cagcgcactg gaccgaggaa ccggctgcgg taagtaaaga tcgaatcgac
     2521 cgtcagcagt tccgtttgcg caaacgcaga aactgctaac cacagaataa tggggataat
     2581 cgctactcga cgtttcataa ctgttttcat atttatttgc atctgtctgt gtttatctgt
     2641 gtccgtctgt ggccctaatc tccgcgcagt atcaacgccg caatcgcaga aatcatctcg
     2701 gcctgttcga taggcttcgc catgtgctgt tgataacccg ccgccaaggc acgctcacga
     2761 tcctttctcg aggcataccc ggtcaacgca atcgccggta tttgcccgcc tttctccggc
     2821 ggcaaggcgc gcaacttatg aatgaattca taaccatctt catcgggcat gccaatgtcg
     2881 ctgatcaaaa tgtcaggcag atcaccggaa aacgcttcga gcgcttcagc ggctgatttg
     2941 gttgccgtaa ctttcgctcc gtaacgtttc agcatcgtgc cgagcagttt gcgcgagtcg
     3001 tcatcgtctt ctaccagcag cacgtgcaca ccgtcgagtc tgatcgctcc ggttcgacta
     3061 cgacggcgtt ctacctcgcg ccgtctctct tcagcacgcg ccggatcacg ccgctcctgc
     3121 gccaacggta actcgaccgt gaaaagagat ccctggccca acccggcact ctcggcatag
     3181 atgctgccgc catgtaactc cacgagatga cgcacaacgg caagacccaa tccaagcccg
     3241 ccctgttttc tcgagatcga gccttcggcc tgccggaaac ggtcgaacac gtacggcaaa
     3301 aactccgctg agatgccttc tcccgtgtcg gcaacggtca aacgcaccgt tgatccgacg
     3361 ctccgcagat cgatcactac aacgccgcca cgctgtgtga acttgatcgc gttcgacagc
     3421 aggttccaga tcacttgccg caaacgattc ggatcgccct tcaccaaaca gtcgctgccc
     3481 aaagactccg ttcgtaattg aattcctttc acgtcggcag ttggccgcag ggcatcgatt
     3541 gctgcttcga cgatgggcgc gagattcagt ggatgcagat cgagacgaag gttgccggtg
     3601 atgatccggg aaacgtcgag caggtcgtct atgagtcggg cctgcgaacg cgcgttgcgc
     3661 tcgatggtct cgattgcccg tgtcgcgctg gatccttcga cttcgccgtt gcgaatcatc
     3721 gaggcccaac caagaatcga cgtcaaaggc gtgcgcagtt cgtgtgaaag tgtggccaga
     3781 aattcatctt tcaggcggtc ggcttcttcg gcatgttggc gtgcgtcgcg ttcgcgaatt
     3841 agtaatgccg cgcgttcctc ttcagctttc tcgcgcgcgg tcacgtcgag cacgagcgaa
     3901 agcacggaga ccatttggcc gctgtcgtca tgcagcactg agttgtacca ctcgcaaaaa
     3961 agcactgacc cgtcgcgggt gtagttacga ttgcgctgta ctccctgaac ttcaacgcct
     4021 tcacgctgac ggttcgtcac cagcgctacg gcgtcgacgt cgtcagcaaa aacaaactgc
     4081 cactcgttga cgtgcttccc gatcacctct tcggccttcc agccaaacaa acgttccgcc
     4141 gattccgacc agcgcgaaac gcgaaagtcg ctgtcccact caaccactgc aagcggtgaa
     4201 ctctcgacgt gaaatgccaa ctgccggtaa gcagtgtttg ccgcgtgttc tgcctgccgg
     4261 cgagcgctga tgtcgctgac gactgcgatg ccgccgctgt tgttgttgtt ctcgtcgagc
     4321 agcggcgagt agcggcacgc gaatacaccg ctgcccgatg gtccataggg atgcttctcc
     4381 gtaaccgttt cctt
//
LOCUS       DASTEY010000493         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_769057, whole genome shotgun sequence.
ACCESSION   DASTEY010000493 DASTEY010000000
VERSION     DASTEY010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769057"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFO72_08630"
     CDS             complement(<1..625)
                     /locus_tag="VFO72_08630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9479396.1"
                     /translation="MAENLKLDSTAPAINVLGVDRLALVNTLDWSIIGTLALLVILGF
                     GLRVTQLGAIGFAEDEMNKLDAIHAYERGDFSANSEHPMVMKLLMWASVRKGPLDNTE
                     AALRLPNALIGALTVIPLFLLTAAFFDRWTALLAAAFWAFGVNAITHNRIGKEDSLLV
                     FFMLFAFFFFVRAKQISPVDKSARANRYAASAVSFGLMIASKYFPHYL"
     gene            876..2012
                     /locus_tag="VFO72_08635"
     CDS             876..2012
                     /locus_tag="VFO72_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479397.1"
                     /translation="MTGLRGGWAAGFAMDNLVTSNIRQRPIRTLVSVAGIALGVSLVM
                     LFTGLARGMSNDLQRRATNVRAELIFMRPGAVQLTATTANLDTRYVDRLKAIDGVEDA
                     LPVIRDFSQSTRGIGIEQVDGVDWDAYAQMNGLRLVDGQAPQNANEVVIDETKARNNN
                     YRVGDTIKPLGEQDYRIAGIYSPESGARVKMTLAGMQEALEVTGKCTFIFVKLRDADQ
                     IEAIAKRIDQELPGNTIQPTRDVFTSFEKSIPYLGVFLRVLVGLAAIVSVLVVMLAMY
                     TTITERTREIGILKAMGASRGYIVGIIEKEAILISVIGLVVGFAVSLVTGYLIHRTYG
                     LVFEYSWTWALVAAAIGLIGGVLGALYPAWRASNLDAVNALAYE"
     gene            complement(1990..3066)
                     /locus_tag="VFO72_08640"
     CDS             complement(1990..3066)
                     /locus_tag="VFO72_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9479398.1"
                     /translation="MAVESPKVHSSEPRAPRNRFAPFGIIFGLLGLLLFAYFVRRAGV
                     GQILAGIQRLGAGFLLVLAISSLRYIVRGLAWTRCVERPHRLPFRDAVAARVMGDSLG
                     NIIPFVSVAVSEPSKAVFVKDRVPLMVGLSALAIENIFYALSVAIFICAGTATLLLSF
                     SLPKPLRYASIAALVTTLVIIPAGYLLIRSQKKFLSGTLRFFSRRGLAPHFIAKIAPR
                     AQTLEERVYGFYDRNRGSFLSIFALDMCFHLAGVVEVYTTLSFISPVAPTLRQAFILE
                     SVNRIINVAFKFIPLRAGVDEGGTGQVSKVLGFARGIGETLAIIRKGRDIFWSGIGLL
                     LIWKKGFSLSGLSEPEPVTHTPAH"
BASE COUNT          839 a          926 c          977 g          690 t
ORIGIN      
        1 cgaggtagtg gggaaaatat ttggacgcga tcatcaagcc aaacgaaacg gcgctggcgg
       61 cgtagcgatt ggcccgggcg gatttgtcga cgggcgagat ctgcttggcg cgcacgaaga
      121 agaagaaggc gaagagcatg aaaaagacca gcaacgaatc ttcttttcca atacggttat
      181 gagtgatggc gttaacgcca aaagcccaaa atgccgcggc cagtaatgcc gtccaacgat
      241 cgaagaaagc ggcggttaat aaaaagagcg gtatgacggt cagagcaccg atcaacgcgt
      301 tcggtaaacg cagcgctgct tcagtgttat ccaatgggcc cttgcgcaca gaggcccaca
      361 tcagcagctt catcaccatc ggatgctcgg agtttgcgga gaagtcgccg cgctcgtaag
      421 cgtgaatggc atccaacttg ttcatctcgt cttccgcgaa accgatcgcg ccgagttggg
      481 taacgcggag accgaagccg agtatgacca gcagcgccag ggtgccgatg atggaccaat
      541 ccagcgtgtt gacgagagct aatcggtcaa cgccgagaac attgatcgcg ggcgcggttg
      601 aatcgagctt taaattctca gccacggtcg aacctgaatc aatactcgta ccggctttgc
      661 ccgaaaagtc ccttggtgaa cttcttctct gtgcacctct gtgcctcggt ggttgttttt
      721 ttccaggaaa tcgctaacca caaaggcaca gagaacacag agaagcgacc aagtcttatt
      781 tttcgggcaa agccactcgt acttgtcgtt tgttagctta gaacaaaatg ctttatggta
      841 atagcccgcc ggttcgtgtc ggaagttaat tacggttgac aggattacgc ggcggatggg
      901 ctgccggttt tgcgatggac aatttagtca cttccaatat tcgtcaacga ccgattcgga
      961 cgctggtcag cgttgccggg atcgcgctcg gcgtaagttt ggtaatgctt ttcacgggcc
     1021 tcgcccgcgg catgtcaaat gaccttcaac gccgtgcgac caacgttcgg gccgaactga
     1081 tcttcatgcg accgggcgcg gtgcagttga cggccaccac cgcaaacctc gacacacgct
     1141 acgtcgaccg tttgaaagca atcgacggcg tggaagacgc gctgcccgtg attcgtgatt
     1201 tttcacaaag cacgcgtggc atcggcatcg agcaggtcga cggcgtcgat tgggatgcgt
     1261 atgcgcaaat gaacgggctc cggctcgttg acggccaggc gccgcagaat gcaaacgaag
     1321 tcgtgatcga cgaaacgaag gcgcgcaaca ataactatcg cgtaggcgac acaataaaac
     1381 cgctgggcga gcaggactat cgcatcgccg gcatttactc accggagtcg ggcgcacgcg
     1441 tgaagatgac gttggccgga atgcaggaag cgctcgaagt caccggcaag tgcacgttca
     1501 tcttcgtgaa gctgcgcgat gcggaccaga ttgaggcaat cgcgaagcgt atcgaccagg
     1561 aactgccggg caacaccatt caaccgacca gagacgtgtt cacgagtttt gaaaaaagta
     1621 ttccttatct cggtgttttt ctgcgtgtcc tggtcggatt ggcagcgatt gtcagtgtgc
     1681 tggtagtgat gctggcgatg tacacgacga tcacggaacg cacgcgcgag attggcatcc
     1741 tgaaagcgat gggtgcgtcg cgaggttaca tcgtgggcat catcgaaaag gaagcgatct
     1801 taatcagcgt catcgggctg gtagtcggat ttgcagtttc gttggttacg ggctatctca
     1861 ttcatcgcac gtacggactg gttttcgagt atagctggac gtgggcgttg gtcgcggctg
     1921 cgattggatt gatcggcggt gtgcttggcg cgctgtatcc ggcatggcga gcgtcaaacc
     1981 tcgacgcggt taatgcgctg gcgtatgagt aacaggttcg ggctcggata atccgctcag
     2041 agaaaaccct tttttccaaa tcaacagcag accaattcca ctccaaaaga tatcgcggcc
     2101 tttgcgaatg atggcgagcg tttcgccgat gccgcgagca aatcccaaca cctttgaaac
     2161 ctgacccgtt ccaccctcgt ccacgcccgc ccgcaacgga ataaacttga acgctacgtt
     2221 gatgatccga ttcacggatt cgagtatgaa cgcctgccgc aacgtgggcg cgaccggact
     2281 gatgaacgac agcgtcgtat aaacttcaac gacacccgca aggtggaagc acatgtccaa
     2341 cgcaaagatt gagagaaagc taccgcggtt gcgatcgtag aagccgtaaa cgcgttcctc
     2401 gagggtttgc gctcgcggcg ctatcttcgc aatgaaatga ggagccaggc ctcgtcgaga
     2461 aaaaaaccgg agggtgccac tcaagaattt tttctgactg cgaatcaaca gataaccggc
     2521 cgggataatt accaaggtcg tgaccagcgc cgcgatgctc gcgtaacgca aaggcttcgg
     2581 caacgaaaag ctcagcagca gcgtcgccgt accggcacag atgaagattg cgactgacaa
     2641 cgcgtaaaag atgttttcga tcgcgagcgc cgagagtccg accatcaacg gcacgcgatc
     2701 cttcacgaac accgccttcg agggttcgct cacggccacg ctcacaaatg gaatgatgtt
     2761 gccgagcgag tcgcccataa cgcgtgccgc aaccgcgtct cgaaacggca gccggtgcgg
     2821 acgttcgacg cagcgtgtcc aggcgagtcc acggacgata taacgaaggc tcgagatcgc
     2881 gaggacgagg aggaaaccgg cgccgagccg ttgaatgccg gcgagaatct ggccaactcc
     2941 ggctcgcctc acgaagtagg caaacaacag gagtcccagc aggccgaata tgattccaaa
     3001 tggagcaaat ctattgcgag gagcgcgtgg ctcactggaa tgaactttcg gactttcgac
     3061 ggccatcgag gtacggtacc acacgcgtta acaattgccg attttcgatt gacggttgcc
     3121 gatttaaggc cgtcgccgtg acggttttac tcaatcggca atcggcaatc gaaaatcggc
     3181 aatctccgcc ctttgcccga accttccgca ctcgattatg atgctacgtt catcctgagt
     3241 ccggcaaaac gagcacatat tcatgagtca atcgcaagca gccgtggcgc agtcgccagc
     3301 cactccgctc acaacacttt ctgaagacga aaccatgttt cgctcgagcg tccgcgaatt
     3361 cgccgaaggc gaactccgcc cgcgcgttga gcacatggac gaaaccgcaa aactcgatcc
     3421 agcgattatt ag
//
LOCUS       DASTEY010000494         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_522103, whole genome shotgun sequence.
ACCESSION   DASTEY010000494 DASTEY010000000
VERSION     DASTEY010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522103"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1036)
                     /locus_tag="VFO72_08645"
     CDS             complement(80..1036)
                     /locus_tag="VFO72_08645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9479399.1"
                     /translation="MQSPNKIEAFAGQRILAEDPRAEKTARACVACGSTDAHKLGIKN
                     ELEIVRCRRCRTIYTPYSPWYSSSYFYLGYYLKPEEIDAPVFVKKRLEEITAEFAPYR
                     ETNHLLDLGCGAGSLLEAARNHGWHAQGVDVSSQAARHVREQGFEVFEGELHEAAFPS
                     AHFDVVTAAELLEHIFEPRALLEEVARILRPGGLFWTTTPHARGLSARVLGLEWRCIW
                     PPEHLQLFSIRGLKSLLRETGFRDLRVDTTGGNPIEIWHALGQTKSAPKTVDQHFDRV
                     LTSYQLNESLMKSRSRRALKSSVNSFLNLSRLGDSMKVFAIR"
     gene            complement(1113..>2143)
                     /locus_tag="VFO72_08650"
     CDS             complement(1113..>2143)
                     /locus_tag="VFO72_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET9479400.1"
                     /translation="SIGFEEQDFSELHHARRIAEHVGADHHEFIVRPDAVEVLPMLVE
                     HYGEPYADSSAVPTYYVARETRKHVTVALNGDGGDESFAGYERYIAMGLTEKYRRVPS
                     LLRESVIKETVNLIPSSPIKRSRVNSAKRLLEVVSRPKIDRYTHWMSVFNEDTKQPLY
                     SDFFREQTMAADATGLLATWFKRANGIGILDAMLLTDQMTYLPNDLLVKVDIATMAVS
                     LEARSPFLDHHVIEFAASLPQKLKLRGLTSKYLLKKVLRKLLPTENLNRRKMGFGVPI
                     GHWFRGKMQPFLREVVLSDKALRRGLFRPEAVRQLVELHTRGERDYSHQLWTLLMLEL
                     WFNRFIDP"
BASE COUNT          471 a          621 c          584 g          467 t
ORIGIN      
        1 gaccgaggtg agcggacgca atctcgtccc caagcctccg gcaagcacga cgcctttcaa
       61 cgttgtcctc cagatcgcgt tatcgaatag cgaaaacttt cattgaatca ccgaggcgac
      121 tgaggtttag aaaactgttt acactgctct tgagcgcccg gcgcgaccgg ctcttcatca
      181 gagattcgtt caattggtaa ctggtcaaca cccggtcgaa atgttggtcc actgtcttcg
      241 gcgccgactt cgtctggccc agcgcgtgcc agatttcgat gggatttccg cccgtcgtat
      301 cgacacgtag gtctcgaaat ccggtttcgc gcaggagcga cttcagaccg cgaattgaga
      361 atagctggag atgctccggc ggccaaatgc agcgccactc caatcccagc acgcgggcgg
      421 acaagccacg ggcgtgcggc gtggtggtcc agaagagccc gccgggccgc agtattcgcg
      481 cgacctcctc gagcaatgct cgcggttcga atatatgttc cagcagttcc gcagcggtta
      541 ccacgtcgaa atgtgctgag ggaaacgctg cttcgtgaag ttcaccttca aaaacttcga
      601 atccctgttc gcgcacatgc ctggcggctt gtgaggaaac atcgacgccc tgcgcgtgcc
      661 agccgtgatt gcgcgcggct tcaagcaaac tacctgctcc gcagccgaga tccagaaggt
      721 ggtttgtttc gcgataagga gcaaactcgg cggtgatctc ttccagtcgt tttttgacga
      781 agaccggcgc atcgatctct tcgggcttga ggtagtaacc gagatagaag taggaactgg
      841 aataccacgg cgagtagggc gtgtagatcg tgcgacaacg gcgacaacgg acaatctcga
      901 gttcgttttt gatgccgagt ttgtgcgcgt cagtggagcc acaggcaacg caggcgcgtg
      961 cggttttttc ggcccgcgga tcctctgcca ggatcctctg ccccgcaaaa gcttcaattt
     1021 tgttcggcga ctgcatacag taggacaggc attcttgcct gtctcagtca gacaaccctc
     1081 tcgcgacaga ccgacaggca ggaatgcctg tcctacgggt ctataaaccg attaaaccag
     1141 agctcgagca tcaacaacgt ccaaagctga tgcgaatagt cgcgttcgcc tcgggtgtgc
     1201 aattcaacca attgccggac tgcttcaggc ctgaaaagac cgcgccgcag cgccttgtcg
     1261 gaaagcacga cctcgcgcag aaacggttgc atcttgccgc gaaaccagtg gccgattgga
     1321 acgccaaaac ccatcttccg acgattgaga ttttcagtag gcaacagctt tcgcagcact
     1381 ttcttcaaca gatacttgct tgtcagtcca cgcagtttca gcttctgcgg caaactcgcg
     1441 gcaaactcga tcacgtgatg gtccagaaac ggcgatcgcg cttccagcga cacggccatc
     1501 gtcgcaatgt cgaccttcac gagcagatcg ttcggcaagt acgtcatctg gtcggtcaat
     1561 aacatcgcgt cgagaatacc gatgccgttc gcgcgcttga accacgtcgc aagtaaaccc
     1621 gtcgcgtccg cagccatcgt ctgttcgcgg aagaagtctg aataaagagg ctgtttcgta
     1681 tcttcgttga agacgctcat ccagtgtgtg taacggtcga tctttggccg cgacacgacc
     1741 tcgagcagac gttttgcgga attaactcgg ctgcgcttga ttggcgaact cggaatcaga
     1801 ttaacagttt ctttaatgac tgattcgcgc agcagcgacg gcacgcgacg atacttttcc
     1861 gtcagtccca tcgcgatgta gcgctcgtac cccgcaaaag attcgtcgcc gccatcaccg
     1921 ttcagcgcca cggtgacgtg cttgcgtgtt tctctggcaa cgtagtaagt cggaaccgct
     1981 gaagagtctg cgtatggttc gccgtaatgc tcgaccagca tcggcaggac ttcaaccgca
     2041 tccggccgca caatgaactc gtgatggtcc gcacccacgt gttcggcgat ccggcgcgcg
     2101 tgatgtagtt cgctgaagtc ctgctcttca aaaccgatcg aga
//
LOCUS       DASTEY010000495         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_982867, whole genome shotgun sequence.
ACCESSION   DASTEY010000495 DASTEY010000000
VERSION     DASTEY010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982867"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VFO72_08655"
     CDS             <1..1103
                     /locus_tag="VFO72_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9479401.1"
                     /translation="IKLGSVTLHFIETPGHTPEGVCILVTDAKAPDQPAKLLTGDTLF
                     IGDVGRPDLAGGKGYTPQMMAEMMYDTLHGKVLKLPDDTEVYPAHGAGSLCGRNMSKE
                     TSSTIGEQRKFNYALKPMTKDEFVRMMTADLPEAPLYFPRDAEINRAGARELSKLQPP
                     QALTPHQVFELRDHILLDVRSAADFGAAHVPGSMNIGLGGQFAMWAGSLIPLNAAIVV
                     IADTNAQVDESVVRLARVGIENVKGYLEGGFQSWLDAGRPIEAIEQVSVAQLKEQMAN
                     TDLQIVDVRRASEYVSGHVPRALNAPLASLDKSLGPLPLKKDKRTAVICAGGYRSSAA
                     ASLLQQQGFSNLLNVSGGTGAWINAGYPVEAP"
     gene            1242..>2671
                     /locus_tag="VFO72_08660"
     CDS             1242..>2671
                     /locus_tag="VFO72_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HET9479402.1"
                     /translation="MLFHPYKPVKVFGITVWPQGMIPRHRDKLAQSIGNAVGNELVSQ
                     QTVFDALFETSFFQNKVEDFVSSYTNELLSRVYPSFIDALPSQARAPILDTISALQYR
                     LAEYIAEMLKSEETSEAIEKFVGNQVDELLERRVGDMVKDESVDRVLNFVEQHLQRLT
                     ATEGFETKVHEFVSGRLDEIARSNAKLAETFTPETIAFIKDRIDQQVPPIVHHLADIA
                     TSPTTRKQIGGLIKHEVDDYYEQLSLFKKIFISRERIHREVDELVNKTLPKRVEEYLR
                     GPAFEQEAEAFLNTTIDNVMARPLDELIGQFDAIRFEDLKSQISNRLIEVLRSETLST
                     SLSIQVTDAIDRLRPQTLGAVLQQLDSNSAAQVKSVLTRSLLGLLAREDTARTINAIM
                     SAQIERLLIAPIGRLGDHVSGNAMERASSALVERITLAARERLPVAIAEFDVGGLVRK
                     KVSDYPLAKLEELVLSVAQHHLKTIEL"
BASE COUNT          637 a          754 c          740 g          540 t
ORIGIN      
        1 agatcaaact gggcagcgtt actctgcact tcatcgaaac gcccggccac acgcctgaag
       61 gagtctgcat cctcgtaacc gacgccaaag cacccgacca acctgcaaaa ctcttgaccg
      121 gcgacacgct tttcatcggc gacgtcggcc gcccggatct cgcgggcggc aaaggctaca
      181 cgccgcagat gatggcggaa atgatgtacg acaccctgca cggaaaagtg ctcaaactcc
      241 ccgacgacac agaggtctat ccggcgcacg gtgcgggctc gttgtgcggg cgcaacatgt
      301 cgaaggaaac atcatcaact atcggcgagc aacgtaaatt caattacgcg ctcaagccga
      361 tgaccaaaga tgaatttgtg cggatgatga cggcggatct tcccgaagct ccgctttact
      421 ttccgcgaga cgctgagatt aatcgcgccg gcgcacgcga actttccaaa ctgcaaccac
      481 cgcaggcatt aacaccgcat caggttttcg aattgcgaga ccacatcctg ctcgacgtcc
      541 gcagcgccgc agatttcggc gccgcacacg ttcccggctc gatgaacatc ggactcggcg
      601 gacagtttgc gatgtgggcc ggcagcctga ttccgttgaa cgctgcgatt gtcgtgatcg
      661 ccgacaccaa cgcgcaggtt gacgagtccg tagttcggct cgcgcgcgtc ggcatcgaga
      721 acgtcaaagg ctatctcgaa ggcggattcc agagttggct cgacgcggga cgtccgatcg
      781 aagcgatcga gcaggtgagt gtcgcgcaat tgaaagagca gatggcaaac acagatttgc
      841 aaatcgtaga cgtgcgacgc gcgtcggaat acgttagcgg tcatgtgcca cgcgcactca
      901 acgcaccgct tgcgagtctc gataaaagtc ttggcccatt gcccctgaag aaagacaaac
      961 gcactgcggt gatttgtgcc ggcggatatc gatcgagtgc ggccgcgagc ttgttacagc
     1021 agcagggttt ttcgaatctg ctgaacgtct ccggtggcac aggcgcatgg ataaacgcag
     1081 gttaccccgt agaagccccc tgagatggta ttaaagtcgg attaatcaac tcaaaggggc
     1141 aaacagttat cgatccgctt acttttaaaa tcctggtcgc aatcggcatc gttgtagttg
     1201 ccaccatcca cggttatggc gcggcgtggc tggcaatctg gatgctgttc catccgtata
     1261 aaccggtcaa ggtttttggc atcaccgtct ggccgcaggg gatgattccc cgccatcgcg
     1321 acaagctggc ccaatcgatc ggcaatgccg tcggcaacga gctcgtgtcg caacaaaccg
     1381 tgttcgacgc gttgtttgaa accagtttct ttcaaaacaa ggtcgaagac tttgtcagct
     1441 cttacacaaa tgagctgctc tcgcgtgtgt atccgtcatt cattgacgcc ttgccatcgc
     1501 aggcgcgcgc gccgattctc gacaccatct ccgcgcttca gtaccggctg gcggaataca
     1561 tcgctgagat gctgaagagt gaggaaacca gtgaagcgat tgaaaagttc gtcggcaacc
     1621 aggtcgacga attactcgaa cgccgcgtcg gcgacatggt taaagacgaa tccgtcgatc
     1681 gcgtgctcaa cttcgtcgag caacatttac aacgactcac cgccaccgaa gggttcgaaa
     1741 caaaagtaca tgagtttgtc tcaggtcgtt tggatgaaat agcccgcagc aacgctaagc
     1801 tcgcggaaac gttcacgccc gaaacgatcg ctttcatcaa agatcgtatc gatcaacaag
     1861 tgccgccgat cgtccaccac ctggctgaca tcgccacgag cccgaccacg cgaaagcaga
     1921 ttggtggctt gataaaacat gaagtggatg attattacga acaactctcg ctcttcaaaa
     1981 agatcttcat ctcgcgcgag cgcattcacc gcgaagtcga cgagctcgtg aacaagacgc
     2041 tgcccaagcg tgtcgaagaa tatttgcgtg gcccggcttt cgagcaggaa gccgaggcgt
     2101 ttttgaatac gacgatcgat aacgtgatgg cgcggccgct cgatgaactg atcggacaat
     2161 tcgatgcgat caggttcgag gatctgaagt cgcagataag caatcgactc atcgaggtcc
     2221 ttcgcagcga aacgttatcg acgtcgcttt cgatccaggt tacggacgca atcgatcggc
     2281 tgcgcccgca aacactcggc gcggtgttgc agcagctcga ttcaaactcc gccgcacaag
     2341 tcaaaagcgt tttgaccagg agtctgctgg gcctgctggc gcgcgaagat acggcgcgca
     2401 ctatcaacgc gatcatgagt gcgcagatcg agcgcctgtt aatcgctccg atcggaaggc
     2461 ttggcgatca cgtttctggc aacgcgatgg agcgtgcgag ctcggcgctg gtcgagcgca
     2521 tcacactcgc agcccgtgaa cgtctgcctg tagcgatcgc ggagtttgac gttggcgggc
     2581 tggtgcgcaa gaaggtgtca gactatcctt tggcaaaact cgaggagctg gtgctctcgg
     2641 tggctcagca ccacctaaaa acaattgaac t
//
LOCUS       DASTEY010000496         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1411527, whole genome shotgun sequence.
ACCESSION   DASTEY010000496 DASTEY010000000
VERSION     DASTEY010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411527"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..>2010
                     /locus_tag="VFO72_08665"
     CDS             210..>2010
                     /locus_tag="VFO72_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9479403.1"
                     /translation="MSPLDPLEILQVIGYSIGALLPLWMLFQLVTHRSNLSNIERILA
                     LLALTMGGWHTSNLVITLHGLFDLGPDTWTTLLRVADTVAVVSITFAYSFLLHVHLHL
                     WANAHSRTLTRSEHLRVYLSYIPTIFLLIAVPRLWIGEYAPMMTKLTFFVLPFAFWIA
                     YVLGLIALTELLIAHRSGNRSEKRIMRTLAASFIGVGLVIFLALAFGLGEGTRAGLYL
                     KTIANLGSLVPSVLLAYYIYRYRYLEIIIEKSLVVVTFATVILTVYVFGIRTIGDWMT
                     QRYGVRPGLVEALLILALALLAAPLRGWLAQRFHKLFEREAALYKEIVARIGSYAGQY
                     KDLSDLLHFVEERTTGALGLRKLKIFIDGSEAWVEQVLEKSKESGWEPIEDEPLLTDQ
                     GFKLAYPLRREDKVEGLLAVDAASASLTPDARSIIGLLADQVAIAIDDSRLFEENLRL
                     ERELAERERLASLGRMAATVAHEIKNPLSALKSIAQVMREDESLRNEYERDLGLIIGE
                     TDRLSQSVTQLLSFARRESPAGQPLSVDELVRSVADLFRANASEQGIVLDCEVRVQAE
                     LTGRSVAALRDALSNLLLNALQATPQGGKVELIA"
BASE COUNT          397 a          591 c          568 g          454 t
ORIGIN      
        1 atgcgaatcc aggcgaatgg catgggcgcc tccaacccgc tggccccaaa caccaccgtc
       61 gccggacagc gaaagaatcg ccgcgtggag ataactttta cgtcgccacc gcgcagcgcc
      121 tcgaactagg acttttttag ccgcagattt tcgcggatac acgctgatct aatccgcgtt
      181 tatctgcgat aatctgcggc tacaatttga tgtccccact tgatccactc gagattttac
      241 aggtcatcgg ctacagcatc ggcgccctgc tcccgctttg gatgctgttc cagctcgtca
      301 cccaccgcag caatctgagc aacatcgagc gcatcctggc tttgctcgcc ctgaccatgg
      361 gcggctggca taccagtaac ctcgtgatca cgcttcatgg tctcttcgac ctcggcccgg
      421 acacatggac cacgctcctc cgcgttgcgg acacggtcgc cgtcgtcagt atcactttcg
      481 cttattcgtt cctgctccac gttcaccttc atctctgggc caacgctcac agccggacac
      541 tcacccgaag cgagcacctg cgcgtttatc tgtcgtacat ccctaccatc tttctgctca
      601 tcgcggtgcc acgtctctgg atcggcgagt acgcaccgat gatgacgaag cttacgttct
      661 tcgtgcttcc attcgctttc tggatcgcgt acgtactcgg gctcattgcg ctgacggagt
      721 tgctgattgc acacaggagc gggaaccgca gcgagaagcg catcatgcgt acgctcgccg
      781 cttcgttcat cggcgtcggt ttggtgattt tcctggcgct tgcctttgga ctgggtgagg
      841 gaacaagagc cggcctttac ctcaagacga tcgccaatct cggctccctt gtcccctcag
      901 tcctgctggc ttactacatc tatcgctatc gctacctcga gatcatcatc gagaagagcc
      961 tcgtcgtcgt aactttcgcg acggtgatcc tgacggttta cgtgttcggc attcgcacca
     1021 tcggcgactg gatgacgcag cgctatggag tcaggcccgg cctggttgaa gcgctcctga
     1081 tccttgctct cgcgttgctc gccgcgccgt tgcgtggttg gttggcccaa cgcttccata
     1141 aactcttcga acgagaagcg gcgctgtaca aagagatcgt agcgcgcatc ggttcttatg
     1201 ccggtcagta caaagatctc tccgatctcc tgcactttgt cgaagagcgc acgaccggcg
     1261 cgcttggctt gagaaagctg aagatcttca tcgacggttc cgaagcctgg gtcgagcagg
     1321 tgctggaaaa atccaaagag agcggctggg agccgatcga ggacgagccg ctgctgaccg
     1381 atcagggctt caagctcgct tacccactgc gtcgtgaaga taaagtagag ggtctgttgg
     1441 cggtcgacgc cgcgtcggca agtctgacgc cggatgcgcg ttcgataatt ggattacttg
     1501 cggatcaggt tgccatcgcg atcgacgact cgagactctt cgaagagaac ctgagactcg
     1561 agcgcgagct tgccgaacgt gagcgtttgg cgtcactcgg acgcatggca gcaaccgttg
     1621 cgcacgaaat caagaatccg ctatcggcac ttaagtcgat tgctcaggtg atgcgtgaag
     1681 acgagagcct gcgaaacgag tacgagcggg atctcggatt gattattggc gaaacggatc
     1741 gtctgagtca gagcgtgacg caactgctga gtttcgcgcg tagagaaagc cccgccggtc
     1801 agcctctcag cgttgatgag ctcgtgcgat ccgtggcgga tctctttcga gcgaacgcca
     1861 gtgagcaggg aattgtgctt gattgcgagg tcagagttca ggcagagcta acgggaagat
     1921 cagttgctgc gttacgcgat gcgttgtcga acctcctgct caacgctctg caggcaacgc
     1981 cgcagggcgg aaaggttgag ctgatcgcgg
//
LOCUS       DASTEY010000497         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_241584, whole genome shotgun sequence.
ACCESSION   DASTEY010000497 DASTEY010000000
VERSION     DASTEY010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241584"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..2073
                     /gene="hflX"
                     /locus_tag="VFO72_08670"
     CDS             469..2073
                     /gene="hflX"
                     /locus_tag="VFO72_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9479404.1"
                     /translation="MSHETRRQVGALIDRKGHVQYVMVGDNRGIELPDFKRVRVASGR
                     FRGLRCVHTHLRGEPLTQDDLTDLALLRLDLMAAIDVDAETGLPGLVRAAHLLPLHAG
                     DLENNASTPDLYSFLQPRMASQLDLDFLALIDSLEEEMTRNRRTARRAEMRDRTILVG
                     VATSSLADAEESMAELAELAASAGVVVQDRIIQRRSEIDPRTVLGKGKLDELIIRALQ
                     LGADMLVFDRELSPAQVRSLSEATDLKVIDRSQLILDIFAQRAQSKEGKIQVELAQLK
                     YLLPRLIAGQDSAFSRLAGGIGGRGPGETKLETDRRRVRDRINRLEKEIEAQRQRRQE
                     RRKGRTRQQLPVISIVGYTNAGKSTLLNALTKSEVHAEQRMFATLDPTSRRLRLPREQ
                     GVIINDTVGFIRALPPDLLSAFRATLEEISDSSLLIHAVDISNPRWAHQVQSVKRILS
                     ELKLADIPTILALNKADLLEPEAVEAAVRQARQIENSEIVVISAISPASLAVLLEKAG
                     ALLARNLSAESAADSNHKSAALSRIA"
     gene            2073..>2444
                     /locus_tag="VFO72_08675"
     CDS             2073..>2444
                     /locus_tag="VFO72_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479405.1"
                     /translation="MALRPRFKTPFTQARDKLLAYAIRPFMFFQPRTRFLIGCATLVT
                     LTTFLLITDYSFGFRQSYKEGEVLASDIVAPADITAIDEAETERRKQAAREAALPVFN
                     YDSGRAEISAQSLRVDWEVIKQ"
BASE COUNT          565 a          735 c          662 g          482 t
ORIGIN      
        1 atgccccgta tcttgacaga attagccata aaagccgttc atgatacgac gccactggat
       61 cgatgtctaa tttcattcac accgggcttt agccctgtgc tacgagctga ggacgaccac
      121 gaaactgttt taacagtttc gcgccgcgac acaagaaacc gttgaaacgg ttggaagtga
      181 acgtgctcgc ggccaccggg ctaaagcccg gtgtgaatga aacggacttg gccaacgaaa
      241 aaactgctta caacctcagc ctttccggaa gcctcaaacc tcagcttgtc acagatgcct
      301 gtggtataat tcgcccccgg aacagaaagg gaaacagtta ttactagcgt tctcggcaac
      361 acgcttgggt taaagccgaa ccagatccgg cggatagaaa agctgtacag tagacgcata
      421 cctcctcggc agattgtcac cccggaattc gctcgccagg tgagcgagtt gtctcacgaa
      481 acccgcagac aagttggcgc cctgatcgat cgcaaaggtc acgtgcaata cgtcatggtg
      541 ggcgacaacc gcggtatcga gctaccggac ttcaaacgcg tgcgcgtcgc gtcgggacgc
      601 ttccgcgggc tgcgctgcgt tcacacacac cttcgcggcg agccactcac gcaagacgac
      661 ctcacggacc tggcgctgtt gcggctcgac ctgatggcgg cgatcgacgt cgatgccgaa
      721 accggattgc ccggactggt tcgtgccgcg cacctgctgc cactgcacgc aggcgatctg
      781 gaaaacaacg cgagcacacc cgatctttat tcgtttctgc aaccgcgaat ggcttcgcag
      841 ttggacctcg acttccttgc gctcatcgat tcgctggaag aagagatgac gcgcaaccgc
      901 cgcaccgcgc gccgggctga aatgcgcgat cgcaccattc tcgtcggcgt tgccaccagc
      961 tcgctcgcag acgcggaaga gtcgatggcc gaactcgcag aactcgcagc atcggcgggt
     1021 gtcgtagtgc aggatcgaat cattcaacgc cgctccgaaa tcgatccgcg cacagttctc
     1081 ggcaaaggca aactcgacga actgatcatt cgcgccctgc aactcggcgc cgacatgctc
     1141 gttttcgatc gcgagctttc accggcacaa gtcagatcac tatctgaagc gaccgatctg
     1201 aaggtcatcg atcgctcgca actaatcctc gacatctttg cccaacgcgc acagtcaaag
     1261 gaaggtaaaa ttcaggttga gttggcccag ttgaaatacc tgttgccgcg actaatcgcg
     1321 ggccaggatt ccgcgttctc tcggctcgcg ggcggtatcg gtggccgtgg tcctggtgaa
     1381 acgaaactgg aaaccgatcg ccgtcgtgtt cgcgaccgca tcaatcgact ggaaaaggag
     1441 atcgaagcgc agcgccaacg ccgccaggaa cggcgcaaag ggcgcacgcg ccagcaactg
     1501 cccgttatct cgatcgtcgg ttacaccaac gccggcaagt ctacgctgct gaatgcgctg
     1561 acaaagagcg aagtacacgc cgagcagcgc atgtttgcga ctctcgatcc gacttcgcga
     1621 cgcctgcgtc tgccgcgcga acagggagtc atcatcaacg acacggtcgg tttcatacgc
     1681 gcgctccctc cggatctcct ttcagctttt cgtgcgacgc tcgaggagat cagcgacagc
     1741 agcctgctga ttcacgcagt cgacatctcg aatccacgct gggcgcatca ggtgcagtca
     1801 gttaaaagaa ttctgtcaga gttgaagttg gcggatattc cgacaatttt ggcgctgaac
     1861 aaagccgact tgctcgaacc cgaagcagtc gaagcggcgg tgcggcaagc gcggcagatc
     1921 gagaacagcg agatcgtggt tatttcggcg attagccccg ctagtcttgc cgtcctcctg
     1981 gaaaaagcag gcgccctgct ggcacggaac ctgagcgctg aatcggctgc ggacagcaat
     2041 cacaagtccg ccgctctgtc gcgcatcgca taatggcact tcggccgcgg ttcaaaactc
     2101 catttaccca agcgcgcgat aagctattag cctatgcgat tcgtccgttc atgttttttc
     2161 aaccacggac gcgatttctt atcggctgcg ccaccctcgt aacgcttact acgtttctcc
     2221 tgatcaccga ttactctttc ggctttcgcc aaagctacaa agaaggggaa gtgctcgcaa
     2281 gtgatatcgt tgcgcccgcg gacatcactg ccattgacga agccgagact gaaagaagaa
     2341 aacaggccgc ccgcgaagca gcacttccgg tgtttaatta cgactccggc cgcgctgaaa
     2401 tctcggccca gagtctgcgt gtcgactggg aggtaatcaa acaa
//
LOCUS       DASTEY010000498         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_587825, whole genome shotgun sequence.
ACCESSION   DASTEY010000498 DASTEY010000000
VERSION     DASTEY010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587825"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFO72_08680"
     CDS             <1..371
                     /locus_tag="VFO72_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HET9479406.1"
                     /translation="LHLNAAETALMCVLMLRGPQTPGELRGNASRLHEFAGLDEIEQT
                     LSALISREPEPLVARLPRQPGQKEVRFAQLLSGEIRVEAPSEPERISPRQTLEQKVDA
                     LVAEVEKLRLQFEEFKKQFD"
     gene            398..>4105
                     /locus_tag="VFO72_08685"
     CDS             398..>4105
                     /locus_tag="VFO72_08685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9479407.1"
                     /translation="MSLELFHPAVARWFARSFPAPTEPQLQAWPEIKKQRHTLIAAPT
                     GSGKTLAAFLSAIDDLVHLGLEGKLDDTTHVIYVSPLKALSNDVQRNLQTPLEGIQKE
                     LQAMGLPEVNIRTLVRTGDTPAAERTAMTKRPPHVVVTTPESLYILLTSEGGRRMLET
                     ARTLIVDEIHAVVGDKRGSHLALSIERLEQLTPNKLVRIGLSATQRPIEEVARFLVGA
                     SNLREDRSPDCSIVDSGHTRQLDIAIELTESPLQAVMSGEVWEEVYDRLAELIRQHKT
                     TLVFVNTRRLAERVARHLGERLGDENIAAHHGSLAREQRLTAEQRLKAGELSALVATA
                     SLELGIDIGDVNLVCQLGSTRSIASFLQRVGRSNHTVAGFPKGRIFPLSRDELVECAA
                     IIDAVRRGELDRLEIPERPLDILAQQIVAAVAAEEWTENDLLAMIRRAYPFRNLEREQ
                     FDAIVRMLAEGFTTKRGRRSTYLHHDAVNQRLRGRRGARLAAITSGGAIPDTADYAVV
                     LEPTDLVIGSVNEDFAIESLQGDIFQLGNASWRVLRVEQGKVRVEDAQGQPPSIPFWL
                     GEAPARSHELSVSVSRLREEVAARTNTESVETGSAVTWLTDEVGLSRAAAEQIVEYLA
                     GAKIVLGVMPSQENLVLERFFDDSGSMQLVLHSPFGSRLNRAWGLALRKRFCRKFNFE
                     LQAAATEDAIVLSLGPTHSFPLDDVFHYLNSKTVRQLLCQALLDAPMWNIRWRWNVTR
                     SLAVLRRRGGKKIPAQLQRMDAEDLLTAVFPDQVACAENLTAGVREIPSHPLVEQTVK
                     DCLEEAMDIDGLERLLTSIERNEKNLFARDVIEASPLAQEILNARPYAYLDDAPLEER
                     RTRAVFQRRWLDPETASDMGKLDQAAIDRVREEVWPEPRNADELHDALVELGFVTEDE
                     GAEWQEFFDELKGDRRAAVLTNGKRLWVAAERLPQITPIFSKASLEPAITAPESVSRT
                     TWTPEDALVEIIRGRLEGLGPVTVEQLVSSSGLDKLAIETALLKLEAEGFVIRGRFTP
                     GATETEWSARRLLARIHSYTLNRLRQEIEPVSSADFIRFLLAWQKVAPDHQMEGPESV
                     RAIIDQLEGFEAPAAAWEGELLPSRLVEYDPSWLDALCLSGEVVWARLTPPVRTASDS
                     GADKPRGAGPVRNTPIALLRRKNFALWSSVFPLLSATATNEFSTTTQVVYDYLKARGA
                     SFFSDIVDNTKLLRSQVEESLAELVANGVVVS"
BASE COUNT          877 a         1198 c         1181 g          849 t
ORIGIN      
        1 tgctgcacct gaatgcagcg gaaacggcct tgatgtgcgt attgatgttg cgtggaccgc
       61 agacgcccgg cgagcttcgc ggcaatgctt ctcgcttgca tgaatttgcc gggctggatg
      121 aaattgaaca aacactctcc gcactaatct cacgcgaacc cgagccgctt gtcgcgcgtt
      181 tgccgcggca acccgggcag aaggaggttc gttttgcgca gttactgtcc ggtgaaatcc
      241 gtgtagaggc gccttcggaa cctgaacgca tatctcctcg tcaaacactc gagcagaagg
      301 tcgacgcgct tgttgcagag gtggagaagt tgcggttaca gtttgaggag ttcaaaaaac
      361 agttcgatta ggggccagtt tgcacggata aacacagatg agtctcgaac ttttccatcc
      421 agcagtagca cgctggtttg cccgcagctt tcccgctccc accgaaccac agctacaagc
      481 atggccggag atcaagaagc aacggcacac gttgatcgct gcgcctaccg gttcaggtaa
      541 aacgctcgct gcatttctct cggcaatcga cgatctggtt catctcggac tcgaaggaaa
      601 actcgacgac actacgcacg tcatctacgt ctcgccgctc aaagcactca gcaacgacgt
      661 tcagcgcaac ctgcaaacac cgctcgaagg aattcaaaaa gagcttcagg cgatgggatt
      721 gccggaagtc aacattcgca ctctcgtgcg caccggcgac acgcccgccg cggaacgtac
      781 cgcgatgact aagcgtccgc ctcacgtcgt ggtgaccact cccgagtcgc tttacattct
      841 cctaaccagc gaaggcggcc ggcggatgtt ggaaacggcc cgaacgctga tcgtcgacga
      901 aattcatgcc gtcgtaggcg acaagcgcgg ttcacacctg gctctttcga ttgaacgttt
      961 agagcaacta actcccaaca aactcgtaag aataggctta tccgccaccc agcgcccaat
     1021 cgaagaagtc gctcgtttcc tcgtcggcgc ctcaaatctc cgcgaagatc gcagtccgga
     1081 ttgctccatc gtcgatagcg gtcacacacg ccagctcgac atcgccatcg aattaaccga
     1141 gtcgccgctg caagccgtca tgtccggcga agtttgggaa gaggtttacg atcggctggc
     1201 agaactgatt cgccagcaca aaacaacact cgtcttcgtc aacacacgcc gtttggccga
     1261 acgcgtcgca cggcacttag gtgaaaggct tggcgacgag aatatcgccg cgcatcacgg
     1321 cagtcttgct cgcgaacaac gtctcaccgc cgaacaacga ctcaaagccg gcgaactgag
     1381 cgcgctcgtc gcgaccgcgt cgctcgagtt gggcatcgat atcggcgacg tcaacctcgt
     1441 ttgccaactc ggttcaactc gctcaatcgc cagttttctc cagcgcgtcg gccgttcgaa
     1501 tcatactgtc gcgggctttc cgaaaggccg catctttccg ctctcgcgtg acgaactggt
     1561 cgagtgtgcc gcaatcatcg acgccgtgcg ccgaggcgag ttggatcggc tcgaaattcc
     1621 ggagcggccg ctcgatattc tggcgcagca gattgttgcg gccgtggcgg cggaagagtg
     1681 gaccgagaac gatctgctcg cgatgatccg ccgagcctat cccttccgaa acctggaacg
     1741 cgaacagttc gacgccatcg tacgcatgct cgccgaagga ttcaccacca aacgcggccg
     1801 ccgttctacc tatctgcatc acgatgccgt caaccagcga ttgcgcggac gcagaggcgc
     1861 gcgactggct gcgatcactt ccggcggcgc gatacctgac acggcggatt acgcagtggt
     1921 gctcgagcca acagatttag ttatcggttc agtcaacgaa gattttgcca tcgagagctt
     1981 gcaaggcgac atctttcaac tcggaaacgc gtcgtggcgc gttttgcgcg tcgagcaggg
     2041 caaagttcgc gtggaagatg cgcagggcca gccgccttcg attccatttt ggttaggcga
     2101 ggcgccggct cgttcacatg aactgtctgt ttcggtctct cgtttgcgcg aggaggttgc
     2161 agcacggact aacaccgaga gtgtcgagac gggttctgcg gtcacgtggt tgacggatga
     2221 ggtcggatta tcgcgagcgg cggccgaaca gattgtcgaa taccttgccg gtgcaaagat
     2281 cgttctcggc gtgatgccgt cgcaggagaa tcttgtcctg gaaaggttct ttgacgatag
     2341 cggcagcatg cagttggttc tgcactcgcc tttcggcagc cgcctgaatc gcgcctgggg
     2401 actcgcgttg cgcaaacggt tctgccgcaa gttcaacttc gagttacaag ccgcggccac
     2461 tgaagacgcg atcgtgcttt cgcttggacc aacgcacagc tttccgctcg acgacgtttt
     2521 tcattacttg aattcaaaga ctgtgcgcca gctactgtgc caggcgctac tcgacgcgcc
     2581 gatgtggaac atccgctggc gctggaacgt cacacgttcg ctcgctgtct tgcgacgtcg
     2641 cggtggtaag aagattccgg cgcagttgca acgcatggat gcggaagacc tgctcacggc
     2701 cgtgtttccc gaccaggtcg catgtgctga aaacctcaca gccggcgtac gcgagattcc
     2761 gtcgcatccg ctcgtcgaac agacggtgaa ggattgtctc gaagaagcga tggacatcga
     2821 cgggctggag cgcctgctga cctcgatcga acgcaacgag aagaaccttt ttgcacgcga
     2881 cgttatcgaa gcttcgcctc tggctcagga gatactcaac gcccggccct acgcctatct
     2941 tgacgacgcg ccgctcgaag agcgccgcac gcgcgcggtg tttcaacgcc ggtggctcga
     3001 tccggagact gccagtgaca tgggtaaact cgatcaggcc gcgatcgatc gcgtgcgtga
     3061 agaggtttgg cccgagccgc ggaatgccga cgaacttcac gatgcgctgg ttgaactagg
     3121 gtttgtaacc gaagacgaag gtgcggagtg gcaggaattt tttgacgaac ttaaagggga
     3181 ccgtcgtgca gcagtactta ccaatggcaa gagactgtgg gttgcggctg agcggttacc
     3241 acaaattaca ccgatttttt cgaaggcgtc gcttgaaccc gccatcaccg ctccggagag
     3301 cgtctcgcgg acaacgtgga ctcccgaaga cgctctcgta gagatcattc gtggccgttt
     3361 ggaaggtctt ggcccggtca cagtggaaca gttggtttcc tcctccggcc tcgacaaact
     3421 ggcgattgaa acagcattgc tgaaactcga agcggaaggt ttcgtgattc gtggccgctt
     3481 cacacctggc gctaccgaaa cggaatggag cgcgcgccgg ctgctcgctc gcatccacag
     3541 ttacaccctc aaccgtttac gccaggagat cgaacccgtc tccagcgccg acttcatccg
     3601 cttcctgctg gcgtggcaga aagtcgcgcc ggaccatcag atggaaggcc ccgaaagtgt
     3661 tcgtgcaatc atcgatcagt tggaaggatt cgaagctccg gcggcggcat gggaaggcga
     3721 actgctgccg tctcgtttgg ttgaatacga tccatcatgg ctggatgcgt tgtgtttgtc
     3781 aggtgaagtc gtttgggcga gattgactcc acccgtacgc accgccagcg acagcggtgc
     3841 ggacaagcca cgtggcgcag gtcctgtacg caatacaccg atcgcactcc tgcggcgcaa
     3901 gaacttcgcg ttgtggagtt cggtctttcc actgttgtcg gcaaccgcaa caaatgagtt
     3961 ttcgacaacg acgcaagtgg tctacgacta tctcaaggcg cgcggagcgt cgttcttcag
     4021 tgacattgtc gataacacta aactgcttcg cagtcaggtc gaagaatctc tggcggaact
     4081 ggttgcgaac ggcgtggtcg tctcc
//
LOCUS       DASTEY010000499         6071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1164274, whole genome shotgun sequence.
ACCESSION   DASTEY010000499 DASTEY010000000
VERSION     DASTEY010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164274"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..2283
                     /locus_tag="VFO72_08690"
     CDS             148..2283
                     /locus_tag="VFO72_08690"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HET9479408.1"
                     /translation="MNFLTYFRAFSYAMIGVAMLALVLAGGLDYGLALAFLVVMIVSW
                     KLEGTRWQLPERFGLAIVLLSIPLFYLDWHYQKSLGEPVERLGVTALAHLIVFLSAVK
                     LLQIKKDRDWVFLYLISFFEVLLAAGLSFSPMFLGTLTLYLLCGLTAVTAFEIQKARR
                     SLRYAETRLLVPPDSRRFKKGRRVWRHTEAARLPFVAITLLVLIFSFALPLFLIAPRS
                     GSAALTRSSGGLTNFIGFSESVTLGQIGHLKQDNGLVMRVRVHSSEAPRGLRWRGVAL
                     DEFTGNGLKKSPEARRPEPFTERAGFFQLGTTESLHRLTTQTFFLEPLESPVLFAAPR
                     VVAVQGDLPFVRLDAEGSIQSRRHDFERLMYKAISDTDEPPAEQLRKDMRPYPMAYQR
                     YLQLPETLDPRIVTLARSMIIDAHARNRYEAAKAIEWQLQRDYGYSLEMKAGGADPLA
                     DFLFNVKTGHCEYFSTAMAVMLRTHGVAARVVNGFLPGEYNETAGAYTVRQSDAHSWV
                     EVYFPETRSWVTFDPTPAAGRIEPVHTGLTAQLHKYAEALELLWFQYVVGYDRQEQRS
                     LAVSLHNQIFDYGRMISTVLTTIRSFLTTNVLTAVIAVVVLLLGVTLLLFGKPLWRFI
                     ARRRGRLLEDGRSYSNVQFYEKLLEVMEQRGLSKDKHQTPLEFAGKLRSSEAMIVTRA
                     YNRVRYGGERLSTSEKREVERALLTLEQE"
     gene            2280..3632
                     /gene="rimO"
                     /locus_tag="VFO72_08695"
     CDS             2280..3632
                     /gene="rimO"
                     /locus_tag="VFO72_08695"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9479409.1"
                     /translation="MKKVGFVSLGCPKNLVDSEVMMGHLKQNGYEITAEASEAETIVV
                     NTCGFIDSAKKESIDAILEAARFKSEGQARRLVVAGCLVERYRDELKAEMPEVDAFIG
                     TNQINDILKVCNPETNVRSLPIVQLGNQSATYLYDESTPRVLATPSHYAFVKIAEGCD
                     RPCAFCFIPQMRGHFRSRRFGSIIAEAHQLAEEGVKEIILVAQDSSRYGEDLGKQDAL
                     ARLLRELARVDGIEWVRVMYTYPTHISDAFLDALAEETNAVKYLDMPLQHASQNVLKL
                     MKRGGTRKSLERLIERVRQRVPGIAVRTTFITGFPGETEADFEELLGFIKNVEFDRVG
                     VFTYSDEEGTPAFDLPDKVPGRIAARRRTALMKQQARISRRKNRARVGEVVRVLFEGE
                     SKESELLWQGRMETQAPDIDGCVLINDVPDGVLPSPGDFVNVEITEAHEYDLIGRISV
                     "
     gene            3635..4180
                     /locus_tag="VFO72_08700"
     CDS             3635..4180
                     /locus_tag="VFO72_08700"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET9479410.1"
                     /translation="MSLKESSDELNPTVVVPADAIPAPKRTRSGLDYVALAISTWGVG
                     YLPLMPGTFGSLVGIGIFLLLGSLLFQLVAVVVITWTGIWAASRTERILRLKDPGKVV
                     VDEVAGQMIALLPLSLFAPSPARTGVIVSFVLFRLFDIFKPYPARRFERLRSGLGIMA
                     DDLVAGVYGAIGTTLLMFSWR"
     gene            4185..4346
                     /locus_tag="VFO72_08705"
     CDS             4185..4346
                     /locus_tag="VFO72_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479411.1"
                     /translation="MDTIKKRQDEATSKETLQDLEEVSEDTDADENEGPSPDGAFDED
                     DELKDADPL"
     gene            complement(4343..5350)
                     /gene="corA"
                     /locus_tag="VFO72_08710"
     CDS             complement(4343..5350)
                     /gene="corA"
                     /locus_tag="VFO72_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET9479412.1"
                     /translation="MEIIVYRKGEQKVTEGFTPNQLPELLQDQQAVIWVDMESPTEAD
                     EQVLLDVFNFHPLTIEDCRENRHYPKIEEFPGYLYFIVHGVRGDTSPDRFNTIELDGF
                     LGSNYVITYHHDYFRSISNVKQLLRTSPIACQRGPAFLLHQILDQVVDYYSPVLDDFD
                     TRISKLEDEIFSLKKPNNEILAQIMDLKRSVLRLRRISIKQMDILLRMSRGEFPLIPD
                     EMRPFYRDIHDHIVRVTDLAESYRDLIGGSLEAYLSVVSNRMNEIMKVLTVFSAVMLP
                     LTFIAGVYGMNFDNMPELHTPYGYYGALAVMAVVAVVMLIFFWRRGWLGGGGGRETPE
                     G"
     gene            5579..>6071
                     /locus_tag="VFO72_08715"
     CDS             5579..>6071
                     /locus_tag="VFO72_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9479413.1"
                     /translation="MSMKIFVADDVSDSGLEPLRAAGFRVEKQTGLTGPSLDQALRDC
                     EGLVVRSETKVTADLLASAHSLRVIGRAGVGVDNIDVPAATIRGIVVMNAPDGNTITT
                     AEHTMALLISLARSIPQANSSLKAGGWERKRFIGVQLHGKTLGIIGLGRIGRVVASRA
                     QAMG"
BASE COUNT         1294 a         1570 c         1816 g         1391 t
ORIGIN      
        1 cgatttgacc gggttgcttc cgcccggggc cgtacaggat gaagaagccg tattgaatgg
       61 aatcgcatat gaccaggcga gcaaccggct gttcgttacc ggaaaacttt ggccgcacct
      121 gttcgagatt aagataaaac gccagtcgtg aacttcttaa cctacttccg agctttctcc
      181 tacgccatga tcggcgtcgc catgctggca ctcgtgctcg cgggagggtt ggactacggg
      241 ctcgcgctgg cgttcctcgt cgtgatgatt gtgagttgga aactcgaagg cacgcgctgg
      301 caattgcccg aacgattcgg cctcgcgatc gtgctgctct cgatcccgct cttctatctc
      361 gactggcact accagaaatc actcggcgag ccagtcgagc ggctgggcgt tacggcgctc
      421 gcgcacctga tcgtttttct ctcggcagtg aagcttttgc agatcaagaa agaccgcgac
      481 tgggtgtttc tttacctgat ctcgttcttt gaagtgctac tggcggcggg attgagtttt
      541 agtccgatgt ttctcggcac gctgacgctt tatttgttgt gtggtctcac tgcggtgacc
      601 gcctttgaga tccaaaaagc gcgccgttcg ttgcgttacg cggagacgcg cttgctggtg
      661 ccgcctgatt cgaggcgctt caaaaaagga cgtcgtgttt ggcgccacac cgaggccgcg
      721 cgtttgccgt tcgtcgcgat cactctgctc gttttgatct tctcgtttgc gttgccgttg
      781 tttttgatcg cgccacgttc tggctccgcg gccctaactc gcagcagtgg cggtctaaca
      841 aactttatcg gcttctccga aagcgtcacg ctcggccaga ttggccatct aaaacaggac
      901 aacggcctgg tgatgcgcgt tcgcgttcac agtagcgaag ctccacgcgg tttgcgctgg
      961 cgtggcgtcg cactcgacga gtttacggga aacggtttga agaaatctcc ggaggcgcgc
     1021 cgtccggaac cgttcacgga acgcgccggg ttctttcagt taggcacgac cgaatcgctc
     1081 catcgtctca caacgcaaac attcttcctc gagccactcg agtcgccggt gctctttgct
     1141 gcgcctcgtg tagtcgcagt gcagggcgat ctgccgttcg ttcggcttga cgctgagggt
     1201 tcgatccagt cgcgccggca tgacttcgaa cgactcatgt acaaggcgat ctccgacacg
     1261 gatgagccgc ccgccgaaca actgcgcaaa gacatgcggc catatccgat ggcgtatcag
     1321 cgctatctcc agttaccgga aacactcgat ccacgcatcg tcacgcttgc acgttcgatg
     1381 attatcgatg cacacgcgcg caaccgttac gaagccgcga aagcaatcga gtggcaacta
     1441 caaagagact acggctactc gttggagatg aaggccggtg gtgcggatcc gctggctgac
     1501 tttctcttta acgtaaaaac aggtcactgc gaatacttct ctacagcgat ggccgtgatg
     1561 ttgcgaacgc acggagttgc ggcgcgtgta gtgaatggat ttttgcctgg cgaatacaac
     1621 gaaacggccg gagcttacac tgtgcgccag agcgatgcgc actcgtgggt tgaagtttac
     1681 tttccggaga cgcgctcgtg ggtcactttc gatccaactc cagcggctgg acgcatcgag
     1741 ccggttcaca caggtctgac tgctcagttg cacaagtacg ccgaggcgct ggagctgttg
     1801 tggtttcagt atgttgttgg ttacgacaga caggagcaac gctcgctcgc ggtgtcgctg
     1861 cacaatcaga tcttcgatta cgggcgcatg atttcgacgg tgttgacgac gatccgcagt
     1921 tttctgacga cgaatgtatt aacggccgtg attgcggtag tcgttttgct gctcggtgtg
     1981 acgcttcttc tgtttggaaa gccgctgtgg cgattcatcg cgcggcgacg cggccggttg
     2041 ctggaagacg ggcgcagtta ttccaacgtt cagttttacg agaagctgct ggaagtgatg
     2101 gagcagcgtg gcctttcaaa agacaagcat cagacgccac tcgagtttgc gggtaagtta
     2161 aggtcgagtg aggcgatgat agtcacgcgc gcatacaacc gcgtgcgata tggcggcgag
     2221 cggctgtcga cttccgaaaa gcgagaggtc gagcgggcgt tgcttacact ggaacaagaa
     2281 tgaagaaagt cggatttgtc agtttaggtt gcccaaaaaa cctcgttgac agcgaagtca
     2341 tgatgggaca cctgaaacag aatggttacg agatcacggc agaagcatct gaggccgaaa
     2401 ctattgttgt aaacacgtgc gggtttatcg attcggctaa gaaggaatcg attgatgcga
     2461 tactcgaggc agcgcgtttt aagtccgaag gtcaggcgag gcgattggtc gtcgccggct
     2521 gtttggtcga acgctatcgc gacgaactga aagcagagat gcccgaagtc gacgctttca
     2581 tcggcaccaa tcagatcaac gacatcctca aagtttgtaa tccggaaact aacgtccgca
     2641 gccttcccat cgtccagctt ggcaatcaga gcgcgacgta tctgtacgac gagtcgacgc
     2701 cacgcgtgct tgcgacgcct tcgcactacg cgtttgtgaa gatcgctgaa ggttgcgatc
     2761 gcccgtgcgc gttctgtttt attccgcaga tgcgtggtca ctttcgcagc cggcgctttg
     2821 gttcgatcat cgccgaggca catcaacttg cggaagaggg cgtcaaggag atcattcttg
     2881 tggcccagga ctcgtcacgg tacggcgagg atctcggcaa gcaggacgca ctcgcgcgat
     2941 tgctgcgcga gcttgcgcgc gtggatggaa tcgagtgggt gcgagtgatg tacacctatc
     3001 cgactcacat cagcgacgcc tttctcgacg cgctcgcgga agagacaaat gcagtcaagt
     3061 atctcgacat gccgctacag cacgcgtcgc agaacgttct gaaactgatg aagcgcggcg
     3121 gcactcgcaa gagtctcgaa cgtttgatcg aacgcgtgcg gcagcgcgtt cccggcattg
     3181 ccgtgcgtac cacgttcatt accgggtttc cgggcgaaac tgaagcagac tttgaagagt
     3241 tgctcggctt tatcaagaac gttgaattcg atcgcgtcgg agttttcacc tattcagatg
     3301 aagagggcac gcctgcgttc gatctgcctg acaaagttcc cggccggatc gctgctcgac
     3361 gccggaccgc tttgatgaaa cagcaggcgc gcatttcgcg tcgcaagaac agagcgcgag
     3421 tgggagaagt cgtgcgcgtg ctttttgaag gcgagtcgaa agagagcgaa ctgttgtggc
     3481 agggaagaat ggaaacacag gcgcccgaca tcgacggttg cgtgctgatc aatgacgtgc
     3541 ccgatggtgt gctgccgtct ccgggagatt ttgtaaacgt cgagatcacg gaggcgcacg
     3601 agtatgatct gattggaagg atttcagtct gacgttgtca ctgaaagaat ccagcgacga
     3661 gttgaatcca actgtggttg tgccggcgga tgcgattccg gctccaaaac gcacgcgcag
     3721 tggtctcgac tacgtggcgc tcgcgatctc gacgtggggt gttggttatc tgccgttgat
     3781 gccggggacg tttggatcgc tggtcggcat aggtattttt ctgctgcttg gctctttgct
     3841 gtttcagctt gtcgcggtgg tagtgatcac ctggaccggc atctgggccg cgtcgcgaac
     3901 cgaacggatc ctgcggctta aagatccggg aaaagtggtg gtcgacgaag tagcgggcca
     3961 gatgatcgcg ttgctgccgc tgtcgttgtt tgcgccgtcg ccggcgagga caggcgtaat
     4021 tgtttcgttt gttctttttc gtcttttcga catcttcaaa ccatatcctg cgcgaaggtt
     4081 tgagcggctg cgtagcggtt tggggattat ggctgacgat ctggtggccg gcgtttatgg
     4141 cgcgataggc acgacgttac tgatgtttag ctggaggtaa agagttggac acgatcaaaa
     4201 agagacaaga cgaagcgacc agtaaggaaa ctttgcaaga cctcgaagaa gtttcggaag
     4261 acactgacgc ggatgaaaac gagggaccaa gtcccgatgg cgcctttgat gaagacgacg
     4321 aactgaagga cgccgatccc ctctaacctt ccggcgtctc gcgacctccg ccaccgccca
     4381 gccagccgcg ccgccagaag aagatcagca ttaccaccgc gaccaccgcc atcacggcca
     4441 gcgctccgta gtaaccatac ggcgtatgca gttccggcat gttgtcgaag ttcatgccgt
     4501 aaactccggc gatgaacgtc aacggcagca tgaccgccga gaagacggtc aacactttca
     4561 tgatctcatt catgcggttt gagacaacgg acagataagc ttcaagcgat cctccgatca
     4621 ggtcgcggta gctctctgcg agatctgtta cgcgtacaat gtgatcgtga atgtcgcggt
     4681 aaaacggccg catctcgtcg gggatcaagg gaaactcacc gcgactcatc cgcagcagaa
     4741 tgtccatctg tttaatggaa atgcgacgca ggcgcagtac gcttcgtttg agatccatga
     4801 tctgggccag gatttcgttg ttgggcttct tcagcgagaa gatctcgtct tcgagcttcg
     4861 agattcgcgt gtcgaagtcg tccagcaccg gcgagtagta gtcgactacc tggtccagga
     4921 tctggtggag cagaaacgct ggtccgcgct ggcaggcaat cggactggtg cgcagcagtt
     4981 gtttgacatt gctgatgctg cggaagtaat catgatggta agtgattacg tagttgctgc
     5041 cgagaaaacc gtccaactcg atcgtgttga agcgatcggg actggtgtcg ccgcgcacac
     5101 cgtgaacgat gaagtaaaga tatccgggaa actcttcgat tttaggatag tggcgattct
     5161 cgcggcagtc ttcgatggtc agcggatgaa aattaaagac gtcgaggagc acttgttcgt
     5221 cggcttcggt cggcgattcc atgtcgaccc agatgactgc ctgctggtcc tgcaaaagct
     5281 ccggcagctg gttgggcgta aagccttcag taaccttctg ttcgcccttt cggtaaacga
     5341 taatctccat cagttatctc ctctgcgcgg tatctgttac catacggcct ttttagtggc
     5401 aagcaaatta catttcgagt actgccgtat tttggatttc tctgtgcctc tgtgcctctg
     5461 tggtgctgac gttcccggag ataaacaacc acagaggcac agaggcacag agaagagctt
     5521 caaattgggg cactatgagt actgataccg attttgagtg ggttaaaagc agcaagcgat
     5581 gtcgatgaag atttttgtag ctgacgacgt gagcgacagc ggtttggagc cgttgcgcgc
     5641 ggccgggttc agggtggaaa aacagactgg tctaacagga ccatcgctgg atcaggcgct
     5701 gagggattgt gaaggcctgg tcgtccgcag tgagacgaaa gtaaccgcgg acttgttggc
     5761 gagtgcgcac agtttacgcg tgattggacg agcgggagtg ggcgtcgaca acatcgatgt
     5821 gcccgccgca acgattcgcg gcattgtggt gatgaacgcg cccgacggca acacgatcac
     5881 tacggccgaa cacacaatgg cgttgttgat ctctctcgcg cgctcgatac cgcaggccaa
     5941 cagcagtttg aaagcaggag gttgggagcg aaaaaggttt attggcgtcc agcttcatgg
     6001 caagacgctc ggcatcattg gcctgggccg tatcggacgc gtcgttgctt cgcgagcgca
     6061 ggcgatgggc a
//
LOCUS       DASTEY010000500         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1559805, whole genome shotgun sequence.
ACCESSION   DASTEY010000500 DASTEY010000000
VERSION     DASTEY010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559805"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2104
                     /locus_tag="VFO72_08720"
     CDS             <1..2104
                     /locus_tag="VFO72_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479414.1"
                     /translation="PQFYDITTDNAKPFYNIYGGTQDNFSFGGPSRTRSASGIVNSDW
                     FVTNGGDGFRSQVDPEDPNTIYAELQNGVLVRFDKRTGERIGIQPQPGRGEDPMRWNW
                     DSPFIISPHLRTRLYFASDKLFRSDDRGDTWQQISGQLSRNLDRDKLPVMGKVWSIDA
                     VAKNASTAHFGNASALAESQKKEGLIYVGTDDGLVQVTEDGGKNWRKIEKFPGVADMA
                     YVSRIITSNHDATTAYVTFDNHQNNDFKPYVLKTTDAGRTWTSLSSNLPKNGPVLAIA
                     EDHVNPDLLFVGTEFGLFFSIDGGQKWVQLKGGLPTIAVRDLNIQKRENDLVLGTFGR
                     GIYVLDNYTPLRLVKPETLRQEAAVFPVKDALMYIQSQPLGGRGKSFQGEAFYTAENP
                     PFGATVTWYLKEAIKTRKQKRQEAEREADRKGLPVGWPTREQLRAEEEEEPPAIVVTI
                     TDSSDRVVRRLTGPVAAGIQRLSWDLRYPAANLSAPPPPDANFGFEPPSGPLVMPGTY
                     KATLAKRVDGVMTQLGPPQEFQVVVEGQENMSVPDRTALVEFQQKAVRLQRAVSGATQ
                     AANALRPRLTAIKRAISETPSLPHRLYEDAMALEKRNTEILRLLSGDSEARRRNMNTP
                     PSINDRVSYVVGAQRMSTSRPTQTQHNQYNAAAQDFEGVLVQLRQLMEVDLARLEKQL
                     EAAGAPWTPGRIPEWRDQ"
     gene            2107..>3238
                     /locus_tag="VFO72_08725"
     CDS             2107..>3238
                     /locus_tag="VFO72_08725"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HET9479415.1"
                     /translation="MKPLKLAGLSVLFLCLSAAHAQTITDKVKGMEKFPGFFTFYWDA
                     KAGKIWLEIDKWNTEFLYVESLPAGIGSNDIGLDRGQLGNSSVVRFDRTGPRVLLVAP
                     NYSFRATSNNPDERLAVKDAFAESTLWGFEVAAEEDGRVLVDATNFYLRDVHQVTNTL
                     QRRQQGTYRLDPARSAFYLPNTKNFPRNTEVETTLTFTGEPAGQFVRRVVPVPEAITV
                     RERHSFVQLPDPGFKPRVFDPRAGFYGIEYMDFATPIEEPITKRFIARHRLQKKDPAA
                     RVSEAVEPIVYYVDRGAPEPVRSALLDGARWWSEAFEAIGYKDAFRVEMMPPEVDPMD
                     VRYNVIQWVHRSTRGWSYGSSLVDPRTGEIIQGRVSLGSLRDR"
BASE COUNT          705 a          928 c          964 g          641 t
ORIGIN      
        1 gccgcagttt tacgacatca cgaccgacaa cgcgaagcct ttctacaaca tttatggtgg
       61 cacgcaggac aacttcagtt ttggtggtcc atcacgcacg cgcagcgcct cgggtattgt
      121 caactccgac tggtttgtca ctaacggtgg cgatggcttt cgctcgcagg tagatccgga
      181 agacccgaat acgatctatg cggagttgca gaacggcgtg ctcgtgcgtt tcgacaagcg
      241 cacgggcgaa cgcatcggca ttcaaccgca gccgggccgc ggcgaagacc cgatgcgttg
      301 gaactgggac tcgccgttca taatcagccc acacctgcgc acacgtctct actttgcttc
      361 agacaaactc tttcgcagcg acgatcgcgg ggacacttgg caacagatca gcggccagct
      421 atcgcgcaat ctcgatcgcg acaagttgcc ggtgatgggg aaagtctgga gcatcgatgc
      481 agtggcgaag aatgcatcga ccgcgcattt cggcaacgcc tcggcacttg ccgagtcgca
      541 gaagaaggaa gggctgatct acgtcggcac tgacgacggc ctggtccaag tgacggaaga
      601 cggcggcaag aattggcgca aaattgaaaa gtttcctggc gttgctgaca tggcctacgt
      661 cagccgcatc atcacgtcca atcacgacgc gaccaccgcg tacgtgactt tcgacaacca
      721 tcagaacaac gacttcaagc cctacgtctt aaagacaact gacgccggcc ggacctggac
      781 ctcgctgagt tcaaacctcc cgaagaacgg accagtgctt gccatcgcgg aagaccacgt
      841 caacccggat cttttgtttg tcggtaccga gtttggacta tttttctcca ttgacggcgg
      901 gcagaagtgg gtgcaactca aaggcggact gccgactatt gcagtgcgcg atctcaacat
      961 tcagaaacgc gagaacgatc tggttctcgg gacgttcggc cgcggcattt acgtgctcga
     1021 caactacacg cctttgcgtc ttgtcaaacc ggaaacactg cgacaggaag cggctgtttt
     1081 tccggtgaaa gatgcgttga tgtacatcca gtcgcaacca ctcggcggtc gcggcaagag
     1141 ctttcagggc gaggcgttct acacggctga gaacccgccg tttggcgcga ctgtgacgtg
     1201 gtacctgaaa gaagcgatca agacgcgcaa gcagaagcgt caggaggctg agcgggaggc
     1261 ggaccgcaag ggtctgccgg tcggttggcc cacgcgcgag cagttgcgcg ccgaggaaga
     1321 ggaggagccg cctgcaatcg tggtcacgat caccgactcg agcgatcgag tggtacgcag
     1381 gctcactggt ccagtcgccg cgggaattca gcggctcagt tgggatctgc gctatccggc
     1441 tgcgaacctc tcggctccac caccaccgga tgcaaatttc ggcttcgaac cgccgtcagg
     1501 accgctggtc atgccgggca cttacaaagc aacactcgcg aagcgagtcg acggtgtaat
     1561 gacacaactt ggaccaccgc aggagtttca ggtcgttgtt gaagggcagg agaatatgtc
     1621 ggtgcctgac cgcacggcgc tggtcgagtt tcagcagaaa gcggtgcgtt tgcaacgagc
     1681 tgttagtggc gcgactcagg ccgcaaacgc tctcaggccg cggctaacgg cgatcaaacg
     1741 cgccatctcc gagacacctt cattaccgca ccgtctgtat gaagacgcca tggcgctgga
     1801 gaaacgaaac accgagatcc tgcgattgtt gagtggcgat tcggaggcgc gccgccgaaa
     1861 catgaacacg cctccctcga ttaacgaccg cgtcagttat gttgtcggcg cgcagcgcat
     1921 gtccacttcg cgtccgacgc agacacaaca caatcagtac aacgcggcgg cacaggattt
     1981 cgagggcgtg ctggttcaat tgcggcaact gatggaagtt gatctggcgc gtttggagaa
     2041 acagttggaa gctgccggcg ccccctggac gcccggccgc atacctgaat ggcgagatca
     2101 gtaactatga aaccacttaa gcttgctggc ctgtccgtgc tgtttttgtg tttgtctgcg
     2161 gctcacgcac aaacgatcac ggataaggtg aaaggcatgg agaagttccc gggcttcttc
     2221 accttctatt gggatgcgaa ggcgggaaag atctggttgg agatcgacaa gtggaacacc
     2281 gagtttctct acgtggaatc actgcccgcc ggtatcggtt caaacgacat cggactcgat
     2341 cgcggacaac tcggtaatag ttcggttgtg cggttcgatc gcactggccc acgcgtcctg
     2401 ctcgttgcgc cgaactattc ctttcgcgcc acgtcaaaca accccgacga acgtcttgca
     2461 gtgaaagacg ccttcgccga atcgacgctt tggggtttcg aagtcgccgc cgaagaagac
     2521 gggcgtgtgc tcgtggacgc caccaacttc tatctgcgcg atgttcatca agttacgaac
     2581 acactgcaac gcagacaaca gggaacgtat cgactcgatc cggcgcgcag cgctttctac
     2641 ctgccgaaca ccaaaaactt ccctcgcaac acggaagtcg aaacaacgct gacgttcacc
     2701 ggcgagccgg cgggccagtt tgtacggcgt gtggttccgg tgcctgaagc gatcacggtt
     2761 cgcgaacggc attcgttcgt gcagttaccc gatcccggtt tcaagccacg agtgttcgat
     2821 ccgcgggccg ggttttatgg catcgagtac atggacttcg cgacgccgat cgaagagccg
     2881 ataacgaagc gcttcatcgc gcgccatcgc ttgcagaaga aagatccggc agcgcgcgtg
     2941 agtgaggccg tcgaaccgat cgtttactac gtcgatcgcg gcgcgccgga gccggtgcgt
     3001 tctgcattac tcgacggcgc gcgctggtgg agtgaggctt tcgaagccat cggttacaaa
     3061 gacgcttttc gcgtggagat gatgccgccg gaagtcgatc cgatggacgt tcgttacaac
     3121 gtgattcagt gggtgcatcg ctcgacgcgt ggctggtcct acgggagctc gctggtcgat
     3181 ccgcgcacgg gcgagatcat tcaggggcgc gtttcgcttg gctcgcttcg cgatcgtc
//
LOCUS       DASTEY010000501         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_802548, whole genome shotgun sequence.
ACCESSION   DASTEY010000501 DASTEY010000000
VERSION     DASTEY010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802548"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFO72_08730"
     CDS             <1..452
                     /locus_tag="VFO72_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9479416.1"
                     /translation="VESLDLNVLIGEAEVIAVPEDYRAIDNEFVLTHCPPGATRLLFK
                     TRNSAFWSEPRLEFRRDFTYLDLEAAKTLVQRGAKLVGIDYLSIEKFHSEKHETHLAL
                     LSHGVVIVEGLNLSDVPAGKYELICLPLRLQSNKGDGAPARVVLRTR"
     gene            456..842
                     /locus_tag="VFO72_08735"
     CDS             456..842
                     /locus_tag="VFO72_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET9479417.1"
                     /translation="MKQADPKYRERVYAMVRRIPSGRVMTYGQIAELLGDGYTPRTVG
                     FCMHSSPDGTPWHRVVNAQGGCSTGKVVLPHDRQQRLLELEGVVFDKNARCDLQQFLF
                     IPKTRSQKPRGASLYKPKTSTRGEKR"
     gene            839..1252
                     /locus_tag="VFO72_08740"
     CDS             839..1252
                     /locus_tag="VFO72_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HET9479418.1"
                     /translation="MSSDSMQQRVPYAVSPPRYSVAHQMVTTAFELPNYRVVHNLGVV
                     RGIVVRSRNIFATIGAGLQTIVGGNITVWTTLCEQTRSDAFDIMIQHATEVGANAVIG
                     ARYDTTEIGQGVTEVLAYGTAVIVEPANPGESFRS"
     gene            1249..1629
                     /locus_tag="VFO72_08745"
     CDS             1249..1629
                     /locus_tag="VFO72_08745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET9479419.1"
                     /translation="MTLDEIPFAKLLGIEADLVEPGHAVLSMKIREELLRNGGIAHGG
                     AIAALIDSAMAFAIVPLLSENERTITVDLTIHYMRPLIGGTARASARVVRAGRRIVTV
                     SAELFDSQEKLAATALSTYLRLSV"
     gene            1653..>2032
                     /locus_tag="VFO72_08750"
     CDS             1653..>2032
                     /locus_tag="VFO72_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9479420.1"
                     /translation="MEDLRKFAHYFRKYKLQLIIGIFCIFTSTAMGLFIPLIVGQAID
                     ENWNEISWTKLTIAAGKVLGASLISGIFLFLQRRILIGMSRNIEYDMRQTFYQHLVNQ
                     PLSFFHEHRTGDLMARATNDLSAV"
BASE COUNT          479 a          576 c          538 g          439 t
ORIGIN      
        1 aagttgagtc tttggacttg aacgtgttga ttggcgaagc ggaagtgatt gcagttccgg
       61 aagactatcg cgccatcgat aacgagtttg tattgactca ttgcccacca ggcgcgacac
      121 gactactttt caaaacccga aactccgcgt tctggagtga acccaggctc gaatttcgca
      181 gagacttcac ttacctcgat cttgaagcag cgaagacgct ggtccagcgt ggcgcaaaac
      241 tggtcggcat cgattatctc tcgatcgaga agtttcattc agagaagcac gagacccatc
      301 tcgcgctgct atcgcacggc gtggtcattg tcgaaggctt gaatctcagc gacgttcccg
      361 caggtaaata tgaactgatc tgtctgccgc tacgtttaca gagcaacaaa ggcgacggcg
      421 ccccggcgcg agtagtcttg agaacgaggt gaacgatcaa acaggcggat ccaaagtatc
      481 gcgagcgagt ttacgcaatg gtcaggcgca tcccgtccgg tcgcgtgatg acttacggcc
      541 agatcgcgga gttgctcggc gacggctaca cgccccgcac cgtcggcttt tgcatgcatt
      601 catcacctga tggaacgccg tggcatcgcg tcgtcaacgc acaaggcgga tgctcgacgg
      661 gcaaggtcgt gctgccacat gacaggcaac aacgtttgct cgagctcgaa ggcgtcgtct
      721 tcgacaagaa cgcgcgctgc gatctgcaac agtttttatt cattccgaaa acgagatctc
      781 aaaagccgag aggtgcaagt ctgtacaaac caaaaacctc cacccgagga gaaaagcgat
      841 gagttcagat tccatgcaac aacgcgttcc ctacgccgtg agcccgccgc gctattcagt
      901 cgcgcatcaa atggtcacca ctgctttcga gttgccgaat tatcgcgtcg tgcataacct
      961 cggcgtcgtg cgcggcatcg tcgtgcgctc gcgaaacatc ttcgccacga tcggagcggg
     1021 actgcaaacc atcgtcggcg gaaacatcac cgtgtggacc acgctgtgcg aacaaacgcg
     1081 cagcgatgct ttcgacatca tgattcaaca cgccaccgaa gtcggcgcga acgcagtgat
     1141 cggcgcacgc tacgacacca cggaaatcgg acagggcgtc acggaagtgc tggcttacgg
     1201 caccgccgtg attgtcgagc ctgccaatcc cggggaaagc tttcgttcat gacacttgat
     1261 gaaattccct ttgccaaatt gcttggcatt gaggctgatt tagtcgagcc cggacatgcg
     1321 gtgctctcaa tgaagatccg cgaagagctt ttgcgaaacg gtggcatcgc acacggcggt
     1381 gccatcgccg ccctgatcga ctcggcaatg gcgttcgcaa tcgtgccgct actgtcagaa
     1441 aacgaacgca cgatcacagt cgatctcacg attcactata tgcgaccgtt aattggagga
     1501 accgcgcgag catcggcgcg cgtggtgcgt gcgggcagga gaatcgtcac agtttcggcg
     1561 gaactcttcg attcacaaga gaaactcgcc gccacggcac tcagcactta cctccgttta
     1621 tctgtctaat ccgcgtttgt tattatcctc gtgtggaaga tttaaggaaa ttcgcgcact
     1681 actttcggaa atacaaactc caactaatca tcggaatatt ctgcatcttc accagcacgg
     1741 cgatgggtct tttcattcct ctcatcgttg gccaggcgat tgacgagaat tggaatgaaa
     1801 tctcctggac gaaactgacc atcgctgccg gcaaagtgct gggcgcaagt ctcatcagtg
     1861 gtatctttct ctttctgcaa cgccggattt tgatcggcat gtcgcgcaac atcgagtacg
     1921 acatgcgaca gacgttttac cagcacctcg tcaatcagcc gctctcgttc tttcacgaac
     1981 atcgcaccgg cgatctgatg gcgcgagcta ccaacgatct cagcgcggtc ag
//
LOCUS       DASTEY010000502         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_390226, whole genome shotgun sequence.
ACCESSION   DASTEY010000502 DASTEY010000000
VERSION     DASTEY010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390226"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2013
                     /locus_tag="VFO72_08755"
     CDS             <1..2013
                     /locus_tag="VFO72_08755"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479421.1"
                     /translation="FPALTRSRTFTTYISAAALSLFAGFVGYAGFRFGVVPELLYTSN
                     LLPRVESVELFLGPVPVAAIVLAACSVAFGLTLNNFGLRRSWHYLLAGLLLLCAASLF
                     VSRFFSFEVLITPVLFSGSLSLLLVQVNRLWHLDRQLMNTLFSSPITVAGSGPGAHQR
                     VMNGLKLLNTCLPLNEAIVFQVDESGYFEAVARFKATAQKQTDTRRNSVWRDGVRLCE
                     RAAASGKLVSQTVGELKELTVAVPLLHEREIAGVLLIRPAAQLVDDDKMLLEAVGSQF
                     ARNLKRESFAKNSARSKSFSYFSQRGSRRKLDALNVLKAITVEQRCEAKALAHINDGV
                     AIAYLDGTVALTNPTILRYAGLTPERARQLDLIGLLNNFRTGIFDEPEIAVRRVLQTG
                     IDYEGELDFDQKDQTLQLRISLLRDQQDTGNAEPIGIAVYVRDLSETKEYEKLKSDMI
                     SLMSHELRTPLTSINGFAELLTADESIPSQAREFVSIIANESQRMSRMINTFLSVTQL
                     QRSDKQEVLKIPLRLDEVVRETIASLQPVAKKKRIRLIEQPAHRIPPVAADKSLITQA
                     VKNLVHNAIKYSPERTTVTVSTALEAETVRVCVEDRGYGIPAESRARVWDKFYRVVRE
                     GQEKDEESTGLGLSFVREVVEQHGGCVELDSEEGRGSKFSFTLPRL"
     gene            complement(2037..>2424)
                     /locus_tag="VFO72_08760"
     CDS             complement(2037..>2424)
                     /locus_tag="VFO72_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET9479422.1"
                     /translation="TIKPSPIHGKGCFATIPFQKRHKIAEYSGEKISNAEARRRAYRR
                     MLRICEVNSRWSLDGSRGGNGTHYINHSCTPNAFMQILHDHILFLALRDITPGEEITI
                     DYESTLHSDEKRCICGSSNCRGTINR"
BASE COUNT          519 a          671 c          683 g          551 t
ORIGIN      
        1 ttcccagcct tgacccgatc ccgaactttt acaacctaca tctctgccgc cgcgctaagc
       61 ctctttgccg gtttcgtggg gtacgccggc tttcgcttcg gggtcgtgcc agaactgctc
      121 tatacctcaa atctcctgcc acgcgtcgaa tcggtcgaac tgtttctcgg acctgttccc
      181 gtcgcggcta ttgttctcgc ggcgtgctca gtcgcttttg gtctcacgct taacaatttt
      241 ggtctccgtc gttcgtggca ttaccttctc gccggactgt tgttgttgtg cgccgcgagt
      301 ctgtttgtct cccgcttttt cagtttcgaa gtcttaatta ctccggtgtt gttcagcggc
      361 agcctctcgt tgttgctggt gcaggtcaat cgcctctggc atctcgaccg gcagttaatg
      421 aacacgctct tcagttcgcc gattacggtc gccggctccg gcccaggcgc gcaccagaga
      481 gtgatgaacg gtttgaagct gctcaacacc tgcctcccct tgaacgaagc aatcgtcttc
      541 caggtcgatg agtctggtta cttcgaagct gtcgcgcgat tcaaagccac ggcacaaaaa
      601 caaacggaca cgcgccgcaa ctccgtctgg cgcgacggag tacgactctg cgaacgagcc
      661 gcagcaagcg gaaagctggt cagtcaaact gtcggagaac tcaaagagtt aaccgtcgcc
      721 gtccctttgc ttcatgaacg tgaaattgcc ggcgtgcttt tgatccggcc cgccgcgcaa
      781 ctcgtcgacg acgacaaaat gctactcgaa gcagtcggtt cgcagttcgc tcgtaacctg
      841 aaacgagaaa gcttcgccaa aaactctgcg cgaagtaagt cgttcagcta cttctcgcag
      901 agaggcagcc gccgcaaact cgacgccttg aatgtgctca aggcgatcac ggtcgagcag
      961 cgctgcgaag cgaaggcgct ggcccacatc aacgacggcg tggcgatcgc ctatctcgac
     1021 ggcaccgtgg cattaacaaa cccgactatt cttcgctatg cgggactcac gcctgagcgc
     1081 gcccggcaat tggacctgat cggtctgctc aacaactttc gcacaggcat cttcgacgaa
     1141 ccggagatcg ccgtgcggcg cgtgctgcaa accggcatcg actacgaagg cgaactcgat
     1201 ttcgaccaaa aagatcagac gcttcaactt cgcatctcgc tgctgcgcga tcagcaggac
     1261 acaggcaatg ccgaaccgat cggcatcgcg gtctatgtaa gagatctcag cgaaacaaaa
     1321 gagtatgaga agctcaagag cgatatgatc tcgctgatgt cgcacgaact gcgcacgccg
     1381 ctcacgagca tcaatggctt cgccgaattg cttaccgcgg acgaaagcat accgtcacaa
     1441 gcgcgagagt ttgtttcgat catcgcgaat gaatcgcaac gcatgtcgcg catgatcaac
     1501 accttccttt cggtcacgca actgcaacgc agcgataagc aggaagtact gaagattcct
     1561 ctgcgtctgg atgaagtcgt gcgcgagacg atcgcgtcgc ttcagccggt ggcgaagaag
     1621 aaacgcattc ggctgattga acaaccggcg caccgtattc cgccggttgc tgccgacaaa
     1681 agtttgatca cgcaggcagt gaagaacctg gtccacaatg cgatcaagta cagtccggag
     1741 cgaactacgg tcacggtttc tacggcgctg gaggcggaaa ctgtgcgcgt gtgtgtcgag
     1801 gatcgtggtt atggtattcc tgctgaatcg agagcgcgtg tgtgggacaa gttctatcgt
     1861 gtcgttcgcg agggacagga gaaagacgag gaatcgaccg gcttgggatt atcgtttgtg
     1921 cgcgaggttg ttgagcaaca cggaggctgc gtcgaattgg attcggaaga gggacgtgga
     1981 tcgaagttca gcttcacgct gccgcgcctg tgagccgcgg ataacacgga tctgcgctaa
     2041 cgattgattg ttccccggca gttgcttgag ccgcagatgc agcgtttctc gtcagaatgc
     2101 aacgtcgact cgtaatcgat cgtgatctct tcacctggcg tgatgtcgcg caaggctaga
     2161 aagagaatgt gatcgtggag tatctgcata aatgcgttcg gagtgcatga gtgattgata
     2221 tagtgcgtgc cgtttccgcc gcggctgcca tcgagactcc agcggctgtt gacttcacaa
     2281 atgcggagca tccgtcgata agcgcggcgg cgtgcttcgg cgtttgagat cttctcaccg
     2341 ctgtattcag cgatcttgtg tcgcttttgg aaaggtatgg tggcgaagca gcctttgccg
     2401 tgtataggtg aaggcttgat tgtt
//
LOCUS       DASTEY010000503         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1478080, whole genome shotgun sequence.
ACCESSION   DASTEY010000503 DASTEY010000000
VERSION     DASTEY010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478080"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..>2517
                     /locus_tag="VFO72_08765"
     CDS             67..>2517
                     /locus_tag="VFO72_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HET9479423.1"
                     /translation="MSDSDRHINFRLMRDHILALQDAEQPRGGKKYWRSLEELADTPQ
                     FREFVAREFPQQAEGWHDPVERRTFLKLMGASLALAGLSGCVFQPPEKIVPYVRQPEE
                     HVPGKALFFATAVPLFGAAIPVLVRSNEGRPTKIEGNPDHPNNRPVDFPAEDPYRDPR
                     GSSATDIFSQASILSLYDPDRSQTLTYREDIRTWTAFVGEMRSALDEQRAKQGAGIRF
                     LTETITSPSLGAQMRELLTALPQAKWHQYDPANRDNARAGAVMAFGQPVNTTYRFDLA
                     QRVLSLDADFLSPMMPGYLRYAREFIARRRVTENNRDMNRLYVIETTPSNTGAMADHT
                     WTVKPSQMEALVRELAAEGAQGPRSLPWMEPLLRDLQQHRGSSIVIAGDGQPPAVHAL
                     AHAMNNSLGNVGKTVFYTDPLEVNSVDQRQSLQELVNDIDAGRVDLLVILGGNPVYNT
                     PVDLKLDLARLNKVNKLRIHLSEYKDETSEICHWHIPAAHYLESWGDERSYDGTVTII
                     QPLIEPLYEGKTAHELLALFSDQYDRRPYDIVKGFWQQESSRIGGTTDFETWWRQSIH
                     DGFIADSAFQPKAVSFNANFANQIPASQAVTSGFDLVYRPDPSIYDGRFANNGWLQEL
                     PKPLTKLTWDNVALLSPNSAQAITGGWKQQNATKGRENYVPMVDIVDQQGRSLRVPVW
                     IMPGQPDGVVTIHLGYGRRRAGRVGGTDDQMIGFDAYRLRTSYEPWFSTGIQLRATTA
                     QHVLATTQLHFNLEDPNFSTERRDVVRSETLADFLHGTKHHDEHVHPSIYPDYDYQNQ
                     AENAPNYKWGMAIDLNNCI"
BASE COUNT          577 a          758 c          679 g          503 t
ORIGIN      
        1 aagattcagg gaaggcgagt tctcgaaagc tgttccacct gtcaccgcta gaccattagc
       61 ttgaagatga gcgatagcga tcgtcacata aacttccgac taatgcgcga tcacattctt
      121 gctttacagg atgcggagca gccgcgcggt ggaaagaaat attggcgaag ccttgaggag
      181 ttggcggaca cgccgcagtt tcgtgagttt gtagcgcgcg agtttcccca acaagccgaa
      241 ggctggcacg acccggttga gcgtcgtacg tttttgaaat tgatgggcgc ttcgttagcg
      301 ctcgccggtt tgagtggctg cgtctttcaa ccgccggaga agatcgttcc ttacgtcaga
      361 caacctgagg aacacgttcc cggtaaagcg cttttctttg cgactgccgt cccgctcttt
      421 ggcgcggcca tacccgttct cgttcgcagc aacgaaggcc ggccgacgaa gatcgagggc
      481 aatcccgatc acccgaataa tcgtcccgtt gattttcctg ccgaagatcc atatcgcgat
      541 ccgcgtggat caagcgccac cgacattttt tcccaagcgt cgatactgag tctctacgat
      601 ccggaccggt cgcagacgct tacttatcgc gaagacattc gcacctggac cgcgtttgtc
      661 ggcgagatgc gctcggcgct ggatgagcaa cgcgcgaaac agggcgcggg tattcgtttt
      721 ctgacggaga caatcacgtc gccttcgctg ggtgcgcaga tgcgggagtt gttgaccgcg
      781 cttccgcaag cgaagtggca ccagtatgac cctgcaaatc gcgacaacgc gcgcgccggc
      841 gcggtgatgg cgtttggaca accggtaaac acgacgtatc gcttcgatct cgcgcagcgc
      901 gttctctcgc ttgatgccga ttttctgtcg ccgatgatgc ctggctatct gcgctacgcg
      961 cgcgagttca tcgcgcgccg tcgcgtgacg gaaaacaatc gcgatatgaa tcggctctac
     1021 gtgatcgaga caacgccgtc gaacaccggc gcgatggcag atcacacctg gacggtaaag
     1081 ccgagccaaa tggaagcgtt ggtgagagag cttgcggccg aaggagctca gggaccgcga
     1141 agccttccct ggatggagcc gctgctgcga gacctccagc aacacagagg ttcgagcatt
     1201 gtgatcgcgg gtgacggcca accaccggcc gttcatgcgt tggcccatgc gatgaacaac
     1261 tcgctcggaa acgttggcaa gaccgtcttc tacacggatc cgcttgaagt taattcagta
     1321 gaccagcgcc agtcgttgca agagctcgta aacgatatcg atgcgggtcg cgtcgatctg
     1381 ctcgtaatcc tcggcggcaa cccggtctac aacacgccgg tcgatctaaa actcgatctc
     1441 gcgcggctga ataaagtcaa caagcttcgc attcatctca gcgaatacaa agacgagact
     1501 tccgaaatct gtcactggca cattccggcc gcgcactatc tcgagtcgtg gggcgacgag
     1561 cgttcttacg acggcaccgt aaccatcatt cagccgctca tcgaaccgct ttacgaagga
     1621 aaaacagccc acgagctact cgctctcttt tcagaccagt acgaccgccg gccctacgac
     1681 atcgtgaaag gtttttggca acaggagtct tcacgaatcg gtggtacgac ggattttgaa
     1741 acgtggtggc gccagtcgat tcacgacggc tttattgccg actcggcatt tcaaccaaag
     1801 gccgtctcat tcaatgctaa ctttgcaaat caaatcccgg cttcccaagc cgtaacatcc
     1861 ggcttcgacc tggtctatcg ccccgacccg tcaatctacg acggtcgctt cgccaacaat
     1921 ggctggcttc aggaactacc gaaaccgctg acgaaactca cgtgggacaa cgtcgcgctt
     1981 ctcagcccga attccgccca ggcaatcacc ggcggctgga aacagcaaaa cgccactaaa
     2041 gggcgcgaaa actacgtgcc gatggtcgat attgtcgacc aacaaggacg ctcgctgcgt
     2101 gttcccgtct ggatcatgcc gggccagccc gacggcgtcg tgactattca tctcggctac
     2161 ggccgccgtc gtgccggacg cgtcggcgga acagacgacc agatgatcgg cttcgacgct
     2221 tacaggctcc gcacgtcata cgagccgtgg ttcagcaccg gaattcaact acgcgcaacc
     2281 accgcacaac acgtgctcgc aacaacgcag ctgcacttca atctagagga cccaaacttc
     2341 tctaccgagc ggcgtgacgt cgtacgctcc gaaacactcg cagattttct ccacggcacg
     2401 aagcaccacg acgagcacgt gcatccatcg atctatccgg attacgacta ccagaaccag
     2461 gcagaaaacg cgcccaacta caagtggggc atggcaatcg atctaaacaa ctgcatc
//
LOCUS       DASTEY010000504         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1478094, whole genome shotgun sequence.
ACCESSION   DASTEY010000504 DASTEY010000000
VERSION     DASTEY010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478094"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..427
                     /locus_tag="VFO72_08770"
     CDS             89..427
                     /locus_tag="VFO72_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9479424.1"
                     /translation="MKPDKPCITATIAEALQHLPGPEGQRFATVFQHGSLEVEIYAPR
                     GTDPQQPHTRDEIYFVAAGTGEYVCGDTRQTFGPTDILFAAAGAVHRFENFSGDLVLW
                     VLFYGPEGGE"
     gene            complement(542..>2057)
                     /locus_tag="VFO72_08775"
     CDS             complement(542..>2057)
                     /locus_tag="VFO72_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET9479425.1"
                     /translation="EFPAGVLQLSAVPGLSLTKIKQLHEELGISSVAELRAAAEGGQL
                     RNLKGFGAKTEQRLLEQISKSKEKPRERLHLHHALSTAERVVEYMKTNRGLVDISFAG
                     SLRRSVETVGTIEIVASGKKPAALVEHFRRLPLILSSVDNEDNNCVAQLADGAVVSFT
                     ATTPDEFATRLFARTGSQAHVDKVLALLTTAPAKPGTAGVSPATNDGSRSASGQPRRL
                     RSQEKRLPRTEEELYERAGMQYVQPELREDQGEVAAAVAGKLPQDLLTLEDIKGMVHC
                     HTTYSDGKHTLEQMVGAAEAMGMKYITITDHSPTAFYAGGLKAEELYRQWDEIDELQS
                     KVKIKILRGTESDILADGGLDYPDRILEQLDCIVASVHARYKMDSRKMTERIINAMRQ
                     PVFKIWGHALGRLIQRRPPFDCDIERILDVIAESKAAIEINGDPYRLDLEPRWVREAR
                     KRKIKFVISVDAHSMGALNNLKFGVAMARRGWVRKNEVLNALPVASFMKAVRPA"
BASE COUNT          430 a          611 c          558 g          458 t
ORIGIN      
        1 gcaggcgaga cgcctgcggt cccagggaag agcacgccca agtaaacagt catttgtagg
       61 tgaaactgct tcactactaa ttaaaacaat gaagccagac aagccctgca ttaccgccac
      121 aatcgctgag gcactccagc acctgcctgg tccggagggc caacgcttcg cgacagtctt
      181 tcagcacggc agtcttgaag ttgagatcta cgcgccgcgc ggtaccgatc cgcaacaacc
      241 gcacacacgt gacgagatct acttcgtcgc cgccggcacg ggcgaatacg tgtgcggcga
      301 cacccgccaa accttcggcc caaccgacat ccttttcgcc gccgcgggcg cagtgcaccg
      361 gttcgaaaac ttctccggag atttggtgct gtgggtgttg ttttacggac ctgaaggtgg
      421 ggagtagaaa ttagcgaaaa agggctcgcg gaaccaacga aaaaacaaac ctcgcgcaaa
      481 ggcgcaaagt tttctaaaaa tctgcgcatc tttgcgccct tgcgcctttg cgcgaatttt
      541 tctaagctgg acgcaccgct ttcataaacg acgcgactgg aagcgcgttc agcacttcgt
      601 tttttctgac ccagccgcgt cgagccatag ccacaccaaa cttcaagttg ttcaacgcgc
      661 ccatcgaatg tgcatcgact gaaatcacga acttgatctt tcgttttcgc gcctctcgca
      721 cccaacgtgg ctccaaatcc agacggtagg ggtcgccgtt gatttcgatc gctgcttttg
      781 attcggcaat cacgtcgaga atgcgttcaa tgtcacagtc aaacggcggc cgccgttgaa
      841 tcaaacggcc cagtgcgtgg ccccagattt tgaaaaccgg ctggcgcatg gcattgatga
      901 tgcgttccgt catcttgcgc gagtccatct tgtaacgggc atgaacgctg gcgacgatac
      961 agtcgagttg ctccaggatg cgatcagggt agtcgagacc gccgtcagcc agaatgtcag
     1021 attcggtgcc gcgcaggatc ttgatcttca ctttactttg cagttcatcg atctcgtccc
     1081 actgtcgata caactcctcg gccttcagtc cacccgcata aaacgcagtc ggcgagtgat
     1141 ccgtgatggt gatgtatttc atgcccatcg cttcggcggc gccgaccatc tgctcgagcg
     1201 tgtgtttgcc gtcggagtag gtcgtgtggc aatggaccat gcccttgatg tcctcgagcg
     1261 tcaacagatc ttgcggcaat ttgccggcaa cagcggctgc gacctcaccc tgatcttcgc
     1321 gcaactcggg ttgcacgtac tgcatgcccg cgcgttcata cagctcttct tcagtccggg
     1381 gcagccgctt ttcctgggac cgcaggcgtc tcggctgccc ggaagcggat cgggagccgt
     1441 cgttagttgc aggcgagacg cctgcggttc caggtttggc cggcgctgtg gtcaagagcg
     1501 ccagcacttt atcaacgtgt gcttgtgatc cggtgcgcgc aaacagccga gtggcaaact
     1561 cgtcgggtgt ggtcgcagtg aaagaaacga ctgcgccgtc ggcgagctgt gcaacacaat
     1621 tattgtcttc gttatctaca ctcgaaagga tcaaaggcaa ccgacgaaag tgttcgacca
     1681 gcgcggctgg tttcttgcca ctcgctacaa tttcgatcgt tcccaccgtc tcgacggaac
     1741 gccgcagcga acccgcaaag ctgatatcca caagaccgcg attggtcttc atgtactcga
     1801 ccactcgctc ggccgtgctc aacgcgtgat gcagatgcaa tcgctcgcgt ggcttctctt
     1861 tcgacttgct gatctgctcg agcaaacgtt gctcggtctt cgcgccgaag cctttcaggt
     1921 tgcgaagttg tccgccctcc gcggcggctc tgagctcggc aacggagctg attccgagtt
     1981 cctcatgaag ctgtttgatc ttagtgagac tcaatccggg caccgccgat agttgaagga
     2041 cccctgccgg aaactct
//
LOCUS       DASTEY010000505         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_983229, whole genome shotgun sequence.
ACCESSION   DASTEY010000505 DASTEY010000000
VERSION     DASTEY010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983229"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFO72_08780"
     CDS             <1..251
                     /locus_tag="VFO72_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479426.1"
                     /translation="IIAEPKALIGFAGPRVIEQTIRQKLPKDFQKSEFLLDHGMIDAI
                     VDRREIRNQVIKLLNFMMNPEIHGESDIERARRAASRN"
     gene            264..1511
                     /locus_tag="VFO72_08785"
     CDS             264..1511
                     /locus_tag="VFO72_08785"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET9479427.1"
                     /translation="MQFDEAVQYLLDLGHETLAIKLGLRNTELLLQVLDNPERAFTVV
                     QIAGTNGKGSTAAMLDSICRTAGIKTGLYTSPHLVSITERIRINGEEITREDFARHTD
                     TVRSVAEKLLAQGEIEALPTFFEQLTAIALLAFREAGVDLAILETGLGGRLDATTAAN
                     AAIVGVTQIAMDHEEYLGPTLTHIASEKAAVIRPGVTAVIAEQRPEALAVLLERCATA
                     GVQPSLDPGTNFYKHFPLSLRGSHQIENASVAVRLAEALRLHGFSISDEQIGLGLAAA
                     EHAGRLELILPFLLDGAHNPAGARALRDYLDEFRPSWLTLIFGAMRDKKLDQIAEILF
                     PIADRLILTPVNNPRTASVVTLAALARRFARATVIESSSSAEAVRTAITQTPNDGLIC
                     VTGSLYLIGETRPMILKLAEELR"
     gene            1508..2083
                     /locus_tag="VFO72_08790"
     CDS             1508..2083
                     /locus_tag="VFO72_08790"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9479428.1"
                     /translation="MTTPNEELPKTISSQKVFEGRVFSVTVDTVREGELAYQREVVHH
                     GGSAVIVPVFDDGTVALVRQYRHPAVRYLLEVPAGTLDDGERPELGAERELQEELGII
                     AGRMEKLSEFFVSPGFCEEKMWVYLATELVQGPQRLEEDEVLEVVRLPISEALEMITS
                     GEIQDAKTIIGLMLAAPKIGVPLFEINYPAV"
     gene            2119..3006
                     /locus_tag="VFO72_08795"
     CDS             2119..3006
                     /locus_tag="VFO72_08795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9479429.1"
                     /translation="MITNEPSTQQTPLPSEPGGDQEQLVEMPPQPVSQTASNEPWIAF
                     LIGIFTWFGSVLLLIFVPAILAMPYAIYRWRTSGLPNIEAITTDKAFIFLSIVAILPS
                     HLLTLGLVWFVVTRAGRYPFWKTVGFEWPRNINPAVGTVTCVAVAVTLLIIGFAVTYF
                     WGGNKTQLDVLIESSIAARFATAFVAVFTAPLVEEVVYRGVLYSGLERALGTINRTIG
                     TAVAFAVVSLLFAGVHVFQYIDNVSVITVITLLSFSLTAVRAYTGKVLPSFIIHLVFN
                     GIQSIIIVLSPFIRWSGSS"
     gene            3020..>4320
                     /locus_tag="VFO72_08800"
     CDS             3020..>4320
                     /locus_tag="VFO72_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9479430.1"
                     /translation="MVYDVVVVGGGIGGLTVAALLAARGLNTCVLERQSQVGGCMARV
                     EFSGREFEPGMGLYTSFGAREIYQRIFAELPVDVPETSLLTRPYVVRLADGADVRLFH
                     DESFYEELRAAFPECADQAIEFYRELKTLESLSTTSPRFQSFINAQLRAFLHTSIDQC
                     SSVDAVAALTRARGPLYEIQGGPATLAERLAESIKRCGGTVRLNTPVLRLAYDETGAA
                     IGVDLLSGEKVLAAKAIVSNLTLWDTYGKLVGLNRTPAEVRKLLNTMQGSGAYVVYAA
                     IEQAAIDRLPAERLLAVTQPVTSEDDRFTEFTLAIHGQTATLKAATDVHEWFTFHASE
                     EDFEESDQAALEHFWTKLHAALPELGAGIEIIETANPRTYYDQTRRKLGMVMGLSSGF
                     PSAEMRFRTFLSNLFVVSDTVSPAPDISSVTQTALSVANAV"
BASE COUNT          931 a         1277 c         1184 g          928 t
ORIGIN      
        1 tgatcatcgc ggagccgaaa gcgctcatcg ggttcgctgg tccacgcgtg atcgaacaga
       61 cgattcggca aaaacttcca aaggacttcc aaaaatcgga gttcctgctg gaccatggca
      121 tgatcgacgc gattgtcgac cggcgcgaga tacgaaatca ggtcatcaaa ctgttgaact
      181 tcatgatgaa ccctgagatt cacggtgagt cggacatcga gcgcgcccgt cgcgctgctt
      241 caaggaatta attcacggat tggatgcagt tcgacgaagc ggttcagtac cttctcgacc
      301 tcggtcacga gacgttagcg atcaagctgg gcctcagaaa taccgaactc ctgctgcaag
      361 tcctcgataa tcccgaacgc gcttttacag ttgttcagat cgccggcacg aatggcaaag
      421 gctcaaccgc cgcgatgctg gactcgatct gtcgcaccgc cggaatcaag actggtctct
      481 acacctcacc gcatctcgtg tcgataaccg aacgcatccg cattaatggt gaggaaatca
      541 cgcgagaaga cttcgcccgg cacacagaca ctgttcgctc ggtcgccgaa aaactgctcg
      601 cgcaaggtga gatcgaagcc ctgccgacgt tcttcgagca actcacagcg attgcgttac
      661 ttgcttttcg cgaagcaggc gttgacttag ccattcttga aactggtctt ggcggccgcc
      721 tcgacgcgac aacagccgcc aacgcagcga tcgtgggcgt cacacagatc gcgatggacc
      781 acgaagagta cctcgggcct accctcactc acatcgcgag tgagaaagcc gccgtcattc
      841 gaccgggagt aaccgcagtt atcgctgaac aaagaccgga agcactcgct gtgttgttgg
      901 aacgctgtgc aacggccggc gtccaaccgt cactcgaccc aggcacaaat ttttacaaac
      961 acttcccgct cagcttacgc ggctcgcacc agatcgaaaa cgcctctgtg gccgttcgtc
     1021 tcgccgaagc cttacgcctc cacggcttct caatttcaga cgaacaaatt gggttgggcc
     1081 ttgcggcggc cgagcacgca ggccgtctcg agctaatcct tcccttcctg ctcgacggcg
     1141 cacacaatcc cgccggagct cgagctctac gcgactacct cgacgagttt cgcccgagtt
     1201 ggctgacact catcttcgga gctatgcgag acaaaaaact cgatcagatc gcggaaatcc
     1261 tttttccaat cgcagaccgc ctcatcttga cgccggtaaa caatccccgc accgcttcag
     1321 tcgtgaccct ggcggccctc gccagacgat tcgcacgcgc aaccgtgatc gaatcaagtt
     1381 ccagcgccga agcagtgcgc accgctatca ctcaaacgcc aaatgatggt ttgatctgtg
     1441 ttaccggctc gctctattta atcggcgaaa cgcgccctat gattttgaaa ctagcggaag
     1501 aactacgatg acgacgccaa acgaggaact gccaaagacg atcagcagcc agaaggtttt
     1561 cgaaggccgc gtgttcagcg ttactgtcga cacggtacgc gaaggcgaac tcgcatatca
     1621 gcgcgaagtg gtccatcacg gcggcagcgc agtcattgtg cccgtcttcg acgatggcac
     1681 ggtcgcactc gtcaggcagt atcgccatcc ggcggtgcgt tacctgctcg aggttccggc
     1741 cggcactctc gatgacggcg aacgtcctga gctcggcgcc gaacgcgaac ttcaggaaga
     1801 actcggcatc atcgccggac gcatggagaa gctgtcggag tttttcgtct ccccgggttt
     1861 ttgcgaagag aagatgtggg tctatctcgc caccgaactc gtacagggac cgcaacgcct
     1921 cgaagaagac gaggtactcg aagtggtgcg gctgccgatc tcagaagcgc tggaaatgat
     1981 caccagcggt gagattcagg acgccaaaac aatcatcggt ctcatgctcg ctgcaccaaa
     2041 aatcggcgtg cctttgtttg agatcaacta tcccgcggtt tgagagttca tccgttccat
     2101 ccgtggtaaa ctcgcctggt gatcacgaac gaaccttcaa cgcaacagac cccgctgccg
     2161 tctgaaccag gaggtgatca ggaacagctt gtcgagatgc cgcctcagcc ggtatcgcag
     2221 acggcgtcaa acgagccgtg gattgcattt ctgatcggga tctttacgtg gttcggcagc
     2281 gtgctgttgt tgatattcgt gccggccata ttggccatgc catacgccat ataccggtgg
     2341 cgaacatccg gcctgcctaa tattgaggcg attactacgg acaaggcatt catcttcctt
     2401 tcgatcgtcg ccatcttgcc cagccacctg ctcacgcttg gattagtgtg gttcgtagtc
     2461 acgcgagcag gtcggtatcc tttctggaaa actgtcggtt tcgaatggcc caggaacatc
     2521 aatccggctg tcggcaccgt cacgtgtgtg gcagttgccg tcacgttgtt aataatcgga
     2581 ttcgccgtaa cgtatttctg gggcggcaac aaaacgcagt tggacgtact catcgaaagc
     2641 tcgatcgccg cgcgctttgc gaccgcgttc gtggcggtct tcaccgcgcc actcgtggaa
     2701 gaggtcgtct accgtggcgt actctactca ggactcgaac gagcgctcgg cacgatcaac
     2761 cgaacaatcg gcaccgccgt ggcttttgca gttgtttcgc tgttgttcgc gggagttcac
     2821 gtgtttcagt acatcgacaa cgtctcggta atcacagtca tcacgctttt gagtttcagt
     2881 ttgacggccg tgcgtgccta caccggcaag gtcctgcctt ccttcatcat ccatctcgtc
     2941 ttcaacggga tccaatcgat aatcatcgtg ctctcgcctt tcattcgctg gagcggctct
     3001 tcgtgattct tcaataccca tggtctatga cgtagtcgta gtgggcggcg gtatcggcgg
     3061 tctgacggtg gccgcgctgc tggccgcgcg cgggctgaat acttgtgtgc tggagcggca
     3121 gtcgcaagtt ggcgggtgta tggcgcgtgt cgagttttcg ggacgcgaat tcgagcctgg
     3181 gatggggttg tacacgtcct ttggcgcccg tgagatttat cagcggattt ttgcggagtt
     3241 gccagttgac gtcccggaaa cgtcgctgct gacgcggccg tacgtcgtac ggctcgcgga
     3301 tggggcggat gtccggttat ttcacgacga gagtttttat gaggagcttc gggcggcgtt
     3361 tccggagtgc gcggatcagg cgatcgagtt ctaccgcgag ctaaagacgc tggagtcttt
     3421 gagcacaacc tcacctcggt ttcagtcttt catcaacgct cagcttcgcg cgtttctaca
     3481 cacctccatc gaccagtgtt catctgtgga cgctgtggct gcgctgacga gagcgcgcgg
     3541 tccgctttac gagattcaag gtggaccggc aacactggcg gagcgtctgg ccgaatcgat
     3601 caaacgctgc ggcggtactg tgcgcctgaa cacgccggtg ctgcgacttg cttacgacga
     3661 gacgggcgcg gcgatcggag tcgatctgct aagcggcgag aaagtgttgg ccgcaaaagc
     3721 aatcgtcagc aacctcacac tctgggacac gtacggcaaa ctggtgggat tgaaccgaac
     3781 accggccgag gtaagaaaac tactcaatac aatgcaaggc tcaggcgcgt acgtcgtcta
     3841 tgccgcgatt gaacaagcgg cgatcgaccg cctgcctgct gaacgtcttc tcgccgtcac
     3901 gcagcccgtc acctccgaag atgatcgatt cacagagttc acgcttgcga ttcacggcca
     3961 aaccgcaacc ctgaaagcgg ccaccgacgt gcacgagtgg tttaccttcc atgcgagcga
     4021 agaagatttt gaggaatcgg accaggcagc gctggaacac ttttggacaa aactccacgc
     4081 cgcacttccg gagcttggcg ccggcatcga aatcatcgaa accgcaaacc cgcgcactta
     4141 ttacgatcaa acgcgccgca aactcggcat ggtgatgggc ctttcatccg gctttccctc
     4201 cgcagaaatg cgtttccgca cgtttctctc gaacctgttc gtagtaagcg acaccgtcag
     4261 ccctgctcca gacatctcat ccgtcacgca aacagcgctt tccgtagcca acgccgtcca
//
LOCUS       DASTEY010000506         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_917771, whole genome shotgun sequence.
ACCESSION   DASTEY010000506 DASTEY010000000
VERSION     DASTEY010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917771"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VFO72_08805"
     CDS             complement(<1..1625)
                     /locus_tag="VFO72_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET9479431.1"
                     /translation="MTPNWQTHAEKTNYRQTPNYADTVAFAQQIAAASPSIEYRGFGH
                     SGQGRELPLLIASETQTFTPEAAREQGKAVVLIQACIHSGEPDGKDAGFALLRDIAIA
                     KTAAGILNNVVLLFIPLYNPDGHERSTPYNRINQNGPESMGWRTTSTYQNLNRDYMKA
                     DTPETRAWLRLWNEWQPDLFIDCHVTDGADYRCNITFHHEHHAGVDDAVVEWERDVFG
                     NRVARATEAAGNVISWYLEFIDNRDLTLGTRDFNGSPRFSTGYVPLRNRPAILIETHM
                     IKDYRSRVIGTYDFLRAALIEVNNDPDRLVRVGREADQRTTDAARVYDPSKLHPLNFE
                     LTDETTPFELKAFKYETEQSDVSGDLRVVYGREPLDLTIPMYQTFRVKTAVAPPLYYI
                     VPVQWTNVIEVLHAHGVRFRSLAEWASIEVECYRFTNVQWPYGPFEGRHMPQFDVERE
                     TSVREFPAGSVVVPVAQPLGKLVLNLLEPQASDSFARWGFFNAIFEEKELAEPYVLEV
                     LAREMRAANPDLKREFEERLQTDADFAANPIER"
     gene            complement(1821..2597)
                     /locus_tag="VFO72_08810"
     CDS             complement(1821..2597)
                     /locus_tag="VFO72_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479432.1"
                     /translation="MEAVSGVFESRQDAQNAINELRRIGIADNRIGLVTPGTDGDELE
                     AGLPVTDTERPGMGKAMGAAVGGAMGAAGGATLGLAVATLAVPGIGPLLAFGMVGAAL
                     LGVVGATAGSAVGDQVEEELGEGIPHEDIFVYEDALRHGRSVLVVYADNDDQADKAED
                     VLDRHGAIDLDELRQHWWNELRDTEREYYHRNGRDFDQDEESYRRGFQAALHPKRRGR
                     AYTEVEQDLRTAYGSELDTAFREGYERGLAHQSRFSEAQQ"
     gene            2753..3124
                     /locus_tag="VFO72_08815"
     CDS             2753..3124
                     /locus_tag="VFO72_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479433.1"
                     /translation="MYDIVEKHWPMSEGDGKAQRKKRPRILAITTDCCTGCAGSPACI
                     EYCPIEACMFWVPDEDHPPFGRIEVDPYLCIGCQKCTSKGPDGAFLDGCPWDAIEMVP
                     TDDWEALHAITLPDAPPAPPA"
     gene            complement(3121..3981)
                     /locus_tag="VFO72_08820"
     CDS             complement(3121..3981)
                     /locus_tag="VFO72_08820"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9479434.1"
                     /translation="MGENGNHPGIRAVEVRRRTPGSGPILILAGLFVVATFLAWYFTW
                     FGRELSDADIAKYLADEKNPRHVQHALVQIQQRIERGDEASKNWYPQLIALSGSSETE
                     FRLTVAWLMGFDNKSEQFHESLLKLLKDPEPLVRRNAALALVRFNDPSGRGELVSILQ
                     PYPVRAPASGIVASSLQAGVTVARTTLLARIQQPDGKVVEVRSPLPGRIHEVLKANGA
                     QVAQGEGVLSLNSDENSVWEALRGLSLIGTTDDLAVIDSYANSTEVSSRIKDQAKLTA
                     AAIKTRIERT"
     gene            complement(3974..4405)
                     /locus_tag="VFO72_08825"
     CDS             complement(3974..4405)
                     /locus_tag="VFO72_08825"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET9479435.1"
                     /translation="MADYITYLPHLNACLNGTSAVLLFTGYAFIRARNVIVHRACQIA
                     ALVVSLLFLASYLTYHYHHGATRFQGTGLVRPIYFTILTSHTILAMVIVPLVILTFYR
                     AFRGDFIRHRRIARITLPLWLYVSVTGVIIYFMLYQIYPHG"
BASE COUNT          970 a         1277 c         1236 g          993 t
ORIGIN      
        1 aagcgctcga taggattggc ggcgaagtcc gcatcggttt gaagccgctc ctcaaactcg
       61 cgcttgagat ccggatttgc cgccctcatt tcacgtgcga gcacttcgag cacgtagggt
      121 tcggccaact ctttctcttc gaagatcgcg ttgaagaaac cccaccgcgc gaacgagtcc
      181 gacgcttgtg gctctaaaag gttgaggacg agtttaccga gcggctgggc cacgggcacg
      241 actactgacc cggcgggaaa ttctctcacg ctcgtctcgc gctcgacgtc aaactgcggc
      301 atgtgccgtc cttcaaatgg accgtacggc cattggacgt ttgtgaatcg ataacattcg
      361 acttcaattg acgcccactc cgccaaagat ctaaatcgca cgccatgcgc atgtaacact
      421 tcgataacgt tggtccactg tacgggcacg atgtaataca gcggcggcgc aactgccgtc
      481 ttcacgcgaa atgtctggta catcggtatc gtcagatcca aaggctcgcg gccgtatacg
      541 acgcgcagat cacctgaaac gtcgctctgt tcggtttcgt acttgaacgc ttttagttcg
      601 aacggcgtcg tttcgtcagt gagttcgaag ttcagtgggt ggagttttga tggatcgtag
      661 acgcgcgcgg cgtcggtggt tcgctggtca gcttcgcgac cgactcgcac cagtcgatca
      721 gggtcgttat tgacttcgat caaagccgcg cgaaggaagt cgtaggtgcc gattacgcgc
      781 gaacgatagt ccttgatcat gtgcgtctcg atcagaatgg cgggacggtt tcgcaacggc
      841 acatagcctg tcgaaaaacg tggcgaaccg ttgaagtctc gggtgccgag cgtaaggtca
      901 cgattgtcga tgaattcgag ataccacgag attacgttgc cggccgcttc ggtcgcgcgg
      961 gcgactctgt tgccgaatac atcgcgctcc cattcaacta ccgcatcgtc gacgcccgcg
     1021 tgatgttcgt ggtggaaggt gatgttgcag cgatagtccg cgccgtctgt cacgtgacaa
     1081 tcgataaaga gatcgggctg ccactcgttc cacaggcgaa gccatgcacg tgtttcgggt
     1141 gtgtcggctt tcatgtagtc gcggttaagg ttttggtagg tgctcgtggt gcgccagccc
     1201 atcgactcgg gaccattttg gtttatgcga ttgtacggcg ttgagcgttc gtgaccgtct
     1261 gggttgtaga gcgggatgaa aagcaagacg acattgttga gaatgccggc ggctgttttc
     1321 gcgatcgcga tgtcgcgtag cagcgcgaag cctgcgtctt tgccgtcagg ttcgccggag
     1381 tgaatgcacg cctggattaa cacgacggct ttgccctgtt cgcgcgcggc ttcgggtgtg
     1441 aacgtttggg tttcggaagc gattagcaac ggcagttcac ggccctggcc gctgtggcca
     1501 aaaccgcgat actcgatcga cggcgaagca gcggcgattt gttgagcaaa agctactgtg
     1561 tctgcgtagt tgggcgtttg gcggtagttg gttttctcag cgtgtgtttg ccagttaggg
     1621 gtcataggac acagataaac acggattatt aacgaagtgt gtgcttacac gaacggcaca
     1681 cgtcgcgacg gcgcgcgttg agagtgccgc aggtttcaca acgaaggtac atccttcgcg
     1741 tgaaccacgt cgtgataatt gcgctgccgc ccaggatcgc gatcaggacg gcagcgttca
     1801 aaaggaaatc cgtcaaaggc ttactgttga gcttccgaga atctgctttg atgagctagc
     1861 ccgcgttcat acccttccct gaacgcagtg tcaagttcgc tgccataagc ggtgcgcaaa
     1921 tcctgttcga cttcggtata agctcttccg cgccgcttcg gatgtaacgc cgcctggaac
     1981 ccgcgccggt agctctcctc gtcctgatcg aagtcacgcc cgttgcggtg atagtactcg
     2041 cgctcagtgt ctcgcagttc gttccaccag tgctggcgga gctcgtcgag atcgatcgcg
     2101 ccatggcggt ctagtacgtc ttctgctttg tcggcttgat cgtcgttgtc ggcgtaaacg
     2161 accagcactg agcggccgtg gcgcagagca tcttcgtaaa caaagatgtc ttcgtgaggg
     2221 atgccttcac ccaactcttc ttcaacctgg tcgcctaccg ccgaaccggc agtagcgccg
     2281 acaactccga gtaacgccgc gccgaccatg ccgaaggcca gcaatggacc aattcccggg
     2341 accgcaagcg ttgcgactgc gagcccgagc gtcgctccgc ccgcagcacc catcgcaccg
     2401 ccgacagcgg cgcccatcgc cttgcccatg ccggggcgtt cagtgtctgt gaccggtaga
     2461 ccagcctcca gttcgtcgcc gtccgttccc ggagttacta aacctattct gttatccgct
     2521 atgccaattc tgcgtaattc attgatcgca ttctgtgcgt cttgtcgtga ttcaaataca
     2581 cctgaaactg cttccattgt ttgctccctc cggtttggta tcgcctactt tctatgcgag
     2641 gcagggtcca ccggcaagca acaacatgcg tcgacacaaa tcgttgagga aatccgcaaa
     2701 gctatgttag agtccaagta cgatttcagc acaccacgag gttttaaagc ggatgtacga
     2761 tatcgtagaa aagcactggc cgatgtcaga gggtgacggc aaggcgcaac gcaagaagcg
     2821 gccacgcata cttgccatca ccaccgactg ctgcaccggt tgtgccggct caccggcgtg
     2881 catcgagtac tgcccgatcg aggcgtgcat gttctgggtg ccggatgagg accatccgcc
     2941 gtttggccga atcgaagtag atccctacct ctgtataggt tgtcagaagt gcaccagcaa
     3001 aggtccggac ggtgcttttc tcgatggatg tccgtgggac gcgatcgaga tggtgccgac
     3061 cgacgactgg gaagcgctgc acgctattac cttgcccgat gcgccgcccg cgccaccggc
     3121 ttaggtccgt tcaatccgtg tttttatagc cgccgcagtt aatttagcct ggtctttaat
     3181 ccggctcgaa acctctgtcg agtttgcata actatcgatc actgcgagat cgtctgtagt
     3241 tcctatcagc gacagtcctc tgagtgcttc ccaaacgctg ttctcgtccg aattgagact
     3301 caacactccc tcgccctgag ccacctgtgc gccgttagct ttcaaaacct cgtgaatgcg
     3361 tcccggcaaa ggagaacgca cttccacgac ctttccatcc ggttgttgaa tacgcgcaag
     3421 caacgtagtc cgcgctactg tcacaccagc ctgcaaacta ctcgcaacaa taccgctggc
     3481 cggagctcgc acgggatacg gctgcaaaat cgaaaccagt tctccacgcc cgctcggatc
     3541 attgaaccgg accagtgcca aagccgcatt gcgtcgcacc agcggctctg ggtctttcag
     3601 taacttcagc aacgactcgt gaaactgctc agacttattg tcgaacccca tcaaccacgc
     3661 cacggtcaac cgaaactccg tctccgagct cccactcagc gcaatcaact gtggatacca
     3721 gttcttcgag gcctcatcgc cccgctcgat tcgctgctgg atctgcacca gcgcgtgttg
     3781 cacgtgccgc gggttctttt catccgcgag gtactttgcg atgtcagcgt ctgacagttc
     3841 tcgtccaaac caggtgaaat accaggccaa aaacgtcgcc accacgaaca gaccggcaag
     3901 gatcagaatc ggaccagaac ccggcgtacg cctgcgaact tcgacagctc gtattcctgg
     3961 atggtttccg ttttcaccca tgtggataga tctgatagag catgaagtaa atgatcacac
     4021 cggtaaccga gacgtaaagc cacaacggca acgtaattcg cgcgatgcgc cggtggcgga
     4081 taaagtctcc gcgaaacgcg cggtaaaagg tgaggattac gagcggcaca atcaccattg
     4141 ccagaatcgt gtggctcgtc agaatcgtga aatagattgg acgcaccagc cccgtgccct
     4201 gaaaacgggt cgcgccgtga tggtagtgat aagtgagata cgaggccagg aacagcagag
     4261 aaaccacgag cgccgcaatc tgacaggcgc gatgcacgat gacgttgcgc gccctgatga
     4321 atgcataacc ggtgaacaga agaaccgcgc tggtgccgtt cagacaagca ttcaaatgcg
     4381 gtagataagt tatgtaatca gccacaagca ttcgacagga ttaccagaat tcaccagaca
     4441 gcataaacga tcatgtcaat tgtgaaatcc tgtcca
//
LOCUS       DASTEY010000507         5880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1593265, whole genome shotgun sequence.
ACCESSION   DASTEY010000507 DASTEY010000000
VERSION     DASTEY010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593265"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFO72_08830"
     CDS             <1..835
                     /locus_tag="VFO72_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9479436.1"
                     /translation="LLVKAGSGVATLGLPDSPGVPAALAENTITVAFNNVPALEKVFV
                     EHPDIAAVIIEPVAGNMGCVPPQPGYLEAVRKLTKANGALLIFDEVMTGFRVARGGAQ
                     ELYNITPDITTLGKIIGGGLPVGAYGGSRDLMNNVAPAGPIYQAGTLSGNPLSMTAGL
                     ITLRRLRDTSVYERLEVASRALCDGLAASANQLGVTTVTNRVGSMWTSFFTDETVIDW
                     QTANRSNRKQYAKFFHAMLDEGVYLAPSQFEAGFVSLAHTDEIIEQTIHAAENAFRVC
                     S"
     gene            complement(847..2637)
                     /locus_tag="VFO72_08835"
     CDS             complement(847..2637)
                     /locus_tag="VFO72_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479437.1"
                     /translation="MQPAEKKPEVVYRRPPDTRTAPPAESNIVAELAKRAFFRAITPL
                     IIGFLLLLALISVLGWRSANQMEEVGNNASVLTTQYFYKQHLLVDLRLKLAQVHNEIR
                     IRHTAQSQKGLKPPFTFNIDNARAELKKTIAVLDSQPVFQDPDWTTLRAELAKYVGLS
                     EDLSRYSIEGHDQLDKVNANIGPLFVQLQNLQQQVSEQALTSQKNARRSILSWTLIAL
                     GVGALVAAGTFWQVQRHFWKMRSSMLEARRERTFTTQLLEGMVSAVAAIDEEDRIRSA
                     NAAFFHIFPNASIGASVLERFAPDDAMKLLETVTAVRVTKATYHGRFVAQVEGEDKSF
                     DVYSSPLAINGDRGQIVTLVDATEAAESERTLRRSESLAAVGQATTQVAHEIRNPLGS
                     IRLGVSMLRDSVTDPEALNTIELVERGIKHLNKLVVDVTQFSRQKELERSAVDLHESL
                     ERSIDLVTDKIVEKNANVERSFARSAIVGQWDAYQLRQVFVNVIANAIDASQENASVT
                     ISTELFSTDGNGDGESRKHYARIRIADQGKGMDKATRDRIFEPFYSTKKRGTGLGLAI
                     VKQIVEQHGGRISVASEPGEGSEFSIDLPI"
     gene            complement(2646..5357)
                     /gene="polA"
                     /locus_tag="VFO72_08840"
     CDS             complement(2646..5357)
                     /gene="polA"
                     /locus_tag="VFO72_08840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET9479438.1"
                     /translation="MSKKTERVFLIDSMSHIFRAFFAPMQNRAAPLATSQGQVTQAVY
                     IFTNMLLKLLREEKPDYIAAVFESREKTFRHETFADYKKNRLEMPDDLSSQLPYIFRL
                     CDVLNITVINSPGYEADDVIGALANQAAAQGLQAVIVSNDKDMCQLVCDPLIVCMRQN
                     SQVVKRKEPVPPVEWCDEAWVEKKFGVTADKLVDLLGLMGDSIDNIPGAPGIGSKGAV
                     QIIQQFGSIENALANWESVKHKTYRESLRNNADLIRQSRELARIRVDLDIPLELDKIK
                     ARPPDRAAAYQLFHELEFAALTREYADAAVEKPATAEAARNYRVVQTKNDLNNLVRTL
                     FDAESIGVAVADSTPAGSGEQECFTELDATRGIAFSTGAGVAAYVDLEKFEGGADQAI
                     APLRELLSNGLVEKSIHDLKRAVGLLDRFNVTLEGVKDDTFLAAYLLDPARSKYELAD
                     LAREALGIENGNTPAANWSEAAWQTVTAADLTAQTARVLRERILEKKLETIYSEIELP
                     LAPLLYRMERAGLKVDPKVLADLSTYLGQELHKITLKIYELAGREFNIGSPKQVGEVL
                     ADLNIDIGRKTSTGRVSTSKAVLEELAQQYELPRLIIEYRELDKLKSVYTDALPQQIA
                     TDGRIHCHLNQTVAATGRLSSSDPNLQNIPIRSELGRRIRRAFVAEKGNKIVSADYSQ
                     LELRLLAHITQDPVMLEAFQKGEDIHARTARLVFGARTDEELREARRFAKIVNFAIAY
                     AIEPWGLSQRVGISRQEAKKVIEDYYNTYKGVRRYMEEVPLRAREHGYVRSIYGRIRP
                     LPGITDRNANIRKAAEREAINMPIQGTASDIVKIAMLHVDDEFKRAGLAARLLMQVHD
                     ELLVEVPEAEVDKVTEILKHEMESAVSLDVPLVADVGVGENWMDAKP"
     gene            complement(5395..>5880)
                     /locus_tag="VFO72_08845"
     CDS             complement(5395..>5880)
                     /locus_tag="VFO72_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9479439.1"
                     /translation="QIDRVAIIDWDVHHGNGTQGIFYDDPSVYFFSSHQYPWYPGTGT
                     RGEKGTGRGLGYTLNLPLRAATPAAEQKRMFEAALEEMSANFSPDLIIISAGFDSHSG
                     DPLGQLLLKDQDFVELTRAVKQWAASSCEGRLVSCLEGGYNLETLGETVRAHVEELSH
                     G"
BASE COUNT         1210 a         1731 c         1611 g         1328 t
ORIGIN      
        1 cctgctggtc aaggccggct cgggtgtggc gacgctcggc ctgccggaca gtccgggggt
       61 tcccgcggca ctagccgaaa acacaatcac agtcgctttc aacaacgttc cggcacttga
      121 aaaagttttt gtagagcacc ctgacattgc cgcggtgatc attgaacccg tcgccggcaa
      181 catgggttgt gtcccgccgc aacccggtta tctcgaagcc gtacgcaaac tcaccaaggc
      241 gaatggagct ttgctgatct tcgatgaagt gatgacgggc tttcgggtcg ctcgcggcgg
      301 cgcgcaggag ctttacaaca tcacgcctga catcaccacg ttgggaaaga tcatcggcgg
      361 cggtttgccg gtgggcgctt acggtggcag ccgtgatttg atgaacaacg tcgctcctgc
      421 tggtccaatc taccaggccg gcacattgtc tggaaatccg ctttcgatga ccgcaggtct
      481 cattaccctc cgccggctgc gggacacatc cgtgtatgaa cggcttgaag ttgcatcgcg
      541 cgcgctttgt gacggactgg ccgcaagcgc aaaccaactc ggagtcacaa ccgttactaa
      601 tcgcgtcggt tccatgtgga caagtttttt cacagacgag acggtgattg actggcagac
      661 agcaaaccgg tccaaccgta agcagtacgc caaattcttc cacgcgatgc tcgacgaggg
      721 agtctacctg gcgccatcac agtttgaggc gggattcgtg agtcttgcgc acacagacga
      781 aattatcgaa cagaccattc acgctgcgga aaacgccttc cgtgtctgtt cgtgaagatc
      841 cgtggcctag atcggaagat cgatgctgaa ctccgatcct tccccaggct cgctcgcaac
      901 actgatccgg ccaccgtgct gctcgacgat ctgtttcacg atcgccagcc ccaaaccggt
      961 gccgcgtttc ttcgtcgaat aaaacggctc gaagattcgg tcgcgcgtgg ctttgtccat
     1021 gcccttgccc tggtctgcta ttcgaatgcg cgcgtaatgc tttcgcgact cgccatcgcc
     1081 attgccgtcg gtgctgaaca actccgtcga aatcgtcact gacgcgttct cctgactcgc
     1141 atcgatggca ttggcaatca cgtttacaaa aacctgtcgc aactggtagg cgtcccactg
     1201 ccccacgatc gcgctgcgcg caaacgacct ttctacgttc gcattcttct cgacaatctt
     1261 gtcggtgacg agatcgatac tccgttcgag cgactcgtgg aggtcaacag cagaacgctc
     1321 cagttccttc tgtcgcgaaa actgcgtgac gtcgacaacg agcttgttca agtgcttgat
     1381 gccgcgctcg acgagttcga ttgtgttcag ggcttcggga tcggtcacgc tgtcgcgcaa
     1441 cattgaaacg ccgagacgaa tgctaccgag cggattgcgg atttcgtggg ccacctgtgt
     1501 tgtggcttgt ccgacggcgg ccagcgactc gctgcggcgc aaggtgcgtt cagattcggc
     1561 ggcttcagtg gcgtcaacca gcgtcacgat ctggccgcga tcgccgttga ttgcgagtgg
     1621 cgacgaatag acgtcgaaag atttgtcttc gccttcgact tgcgcgacga aacggccgtg
     1681 gtaggtggct tttgtgacgc ggacggcggt aaccgtttcg agcagtttca tcgcatcgtc
     1741 gggagcgaag cgttcgagca ccgaagcacc tatgctggcg ttaggaaaga tatggaagaa
     1801 agcggcgttg gcgctgcgga tgcgatcctc ttcatcgatc gcggcgacgg cgctgaccat
     1861 accttcgagc aactgagtgg tgaaagtgcg ttcccggcgg gcctcgagca tgctgctgcg
     1921 catcttccaa aagtgacgtt gcacctgcca gaatgtcccg gccgcaacca gagccccgac
     1981 gcccagcgcg atcaaagtcc aactgaggat cgatcgccgc gcattctttt gtgaggtcag
     2041 cgcctgttca ctaacctgtt gttgtagatt ctgtaactga acgaacaacg gacctatatt
     2101 cgcgttcacc ttgtcgagct gatcgtggcc ctcaatgctg taacgcgaca agtcctcgct
     2161 caaccccacg tacttcgcca actcggctcg gagtgtagtc caatccggat cttggaagac
     2221 tggctggctg tccaatacag caatcgtctt cttcaactcc gccctcgcgt tatcaatgtt
     2281 gaaagtgaac ggtggcttga gtcccttctg cgattgggcg gtgtgtcgaa tgcgaatctc
     2341 gttgtgcact tgagctaact taaggcgcag atcgaccaaa aggtgttgct tgtaaaagta
     2401 ttgagttgtc aggacactcg cattattccc tacttcttcc atttggttgg cgctgcgcca
     2461 gcccaataca gaaatcagtg cgagcaaaag caggaagccg ataatcaaag gcgtgatcgc
     2521 ccgaaaaaag gcacgcttcg ccagctcggc gacgatgtta ctttccgccg gaggtgcggt
     2581 gcgcgtatcg gggggccgcc ggtaaactac ttcaggtttc ttttctgcgg gttgcatccg
     2641 aatcatcagg gcttcgcgtc catccaattc tcaccaacgc ccacatcggc aactagtggc
     2701 acgtcgagcg agactgccga ttccatctcg tgtttgagta tctctgtcac cttatcaact
     2761 tctgcttccg gcacctcaac caacagctcg tcgtgcacct gcatcaacaa ccgcgccgcc
     2821 aatcccgcac gcttgaactc gtcatcaacg tgcagcatcg cgatcttcac gatgtcgctc
     2881 gcggtgcctt gtatcggcat gttgatggct tcccgttccg cagccttgcg aatgttcgcg
     2941 ttgcgatcgg tgatgccggg cagcggacga atgcgcccgt agatcgaccg cacgtaacca
     3001 tgctcgcgtg cccgcaacgg tacttcctcc atgtaacggc gcacgccctt gtacgtgttg
     3061 tagtagtcct cgataacttt ctttgcttcc tgccgggaga taccaacgcg ttgcgagagt
     3121 ccccacggtt caatggcgta ggcaatggcg aagttcacta tctttgcgaa gcggcgtgct
     3181 tcgcgaagtt cttcgtcagt gcgtgcgcca aagacaagtc gcgcggtgcg ggcgtgaatg
     3241 tcttcgccct tttgaaatgc ttcgagcatc accggatcct gcgtgatgtg cgcgagcagt
     3301 cgcaattcga gttgcgaata gtcagccgag acgatcttgt tccccttctc cgctacgaaa
     3361 gcgcgacgga tacgacggcc cagctcgctg cgaatcggaa tgttttgcag attcggatcg
     3421 ctcgacgaca aacggcccgt agcggcaacc gtctgattca agtgacagtg aatgcgcccg
     3481 tccgtggcga tctgttgcgg caaggcgtcg gtgtaaacac tcttcagctt gtctagctcg
     3541 cgatactcga taatcaaacg cggcaactcg tactgctgcg ccagctcctc gaggaccgcc
     3601 ttgctggtcg agacgcgtcc tgttgaagtc ttgcggccga tatcgatgtt tagatcggcg
     3661 agcacctcgc cgacctgttt cggcgatccg atattgaact cgcggccggc gagttcatag
     3721 atcttcaaag tgatcttatg cagctcctgg ccgaggtagg tcgaaagatc ggcaagaact
     3781 ttgggatcga ctttcaatcc cgcgcgctcc atgcgataga gcaacggcgc cagcggtagt
     3841 tcgatttctg agtagatcgt ttcgagcttc ttctcgagta tgcgttcgcg caacacgcgc
     3901 gcggtttgcg ccgtcaggtc cgccgcagta acagtttgcc aggccgcttc ggaccaattt
     3961 gccgccggcg tgttgccatt ctcgatgccg agtgcctcgc gcgcaagatc cgccagctcg
     4021 tatttgctgc gtgcgggatc gagcaggtag gcggcgagaa acgtatcgtc tttcacgcct
     4081 tcaagcgtga cgttgaaccg atcgagtaaa ccgactgctc gcttcaggtc atgaatcgac
     4141 ttttcgacca gaccattcga cagaagttca cgcagcggcg cgattgcctg gtcggccccg
     4201 ccttcgaact tctccaggtc gacatacgcg gccacaccgg cgccggttga gaaagcaatg
     4261 ccgcgcgtcg catcgagttc agtgaaacac tcctgctcgc ctgagcctgc gggtgtcgag
     4321 tccgcaacag cgacgccaat actttccgcg tcgaacagag ttcgaacgag gttgtttaga
     4381 tcgttcttcg tttggacgac gcgataattt cgcgccgctt cggccgtcgc tggtttttcg
     4441 accgccgcat ccgcgtactc gcgagtcaat gcggcaaatt ccaactcgtg aaacagttga
     4501 tacgccgcgg cgcgatcggg ggggcgcgcc ttgatcttgt ccagctcgag tggaatgtcg
     4561 aggtcgacac gaatgcgcgc caactcacgc gactgcctga tcaggtccgc attattgcgc
     4621 aacgactcgc gatacgtctt atgtttcact gactcccagt tagccagcgc attctcgatc
     4681 gaaccaaact gctgaataat ctgtaccgcg cctttcgaac cgatccccgg agcgccaggg
     4741 atgttgtcga tcgaatcgcc cattaagccc agcagatcaa ccagcttgtc ggcagtgacg
     4801 ccaaacttct tttccaccca cgcttcatca caccattcga ccggcggcac aggttctttg
     4861 cgtttcacga cctgagagtt ttgccgcatg caaacgatca gcggatcgca aacgagctgg
     4921 cacatgtctt tgtcgtttga cacgatcact gcctggagac cttgcgccgc agcctggttg
     4981 gctaacgctc cgatcacgtc gtccgcttcg taaccgggag agttgatcac cgtgatgttc
     5041 agcacgtcgc aaaggcgaaa gatatacggc aactggctcg aaagatcgtc aggcatttcg
     5101 agccggttct ttttgtaatc ggcaaacgtc tcgtggcgaa aagtcttttc gcgcgattca
     5161 aacacagcgg cgatgtagtc aggcttctct tcgcgcagaa gtttgagcag catgtttgtg
     5221 aagatgtaaa cggcctgtgt cacctggcct tgcgaagtcg ccaggggcgc agcgcgattc
     5281 tgcatcggcg cgaagaacgc acgaaagatg tgcgacatcg agtcgatcaa aaacacccgc
     5341 tctgttttct tactcatatc agcgacaatt tacgcatttg agcgaaggtc cgtgttatcc
     5401 gtggctcagt tcctcaacat gcgcgcgcac ggtctcaccc aacgtctcta aattgtatcc
     5461 accctcgaga caggaaacga gacgtccctc gcaactactc gcggcccatt gcttcacggc
     5521 gcgcgtcaac tcaacgaagt cttgatcctt gagcaacaac tggccgagcg gatcgccgga
     5581 atgcgagtcg aaacccgccg agatgatgat taaatcagga ctgaaattag ccgacatctc
     5641 ctccagcgcc gcttcaaaca ttcgcttctg ttcggccgcc ggcgtcgccg cacgcaacgg
     5701 cagattcaac gtgtaaccca gaccgcgacc ggttcccttc tcgccgcgcg ttccggttcc
     5761 gggataccac ggatactggt gcgacgagaa aaagtagacg gacggatcgt cgtagaagat
     5821 tccctgcgta ccgttgccgt gatgcacgtc ccaatcgatg atcgcgacgc gatcgatctg
//
LOCUS       DASTEY010000508         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1231256, whole genome shotgun sequence.
ACCESSION   DASTEY010000508 DASTEY010000000
VERSION     DASTEY010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231256"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFO72_08850"
     CDS             <1..470
                     /locus_tag="VFO72_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479440.1"
                     /translation="KIEKLWPLMAAESKQAHDIRKFEEVGTHSAAPRLAIGEAMLFHN
                     GMGGKRKEARLRYLSRYWMNRLKDVPKVRFNTSFDPNQSCAIANVEIVGLDPTAIGAY
                     LFNKHRIFTTPILHDEFKGIRITPNVYTTLGELDRFCNEMEKIARKGLPNPTV"
     gene            545..817
                     /locus_tag="VFO72_08855"
     CDS             545..817
                     /locus_tag="VFO72_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479441.1"
                     /translation="MQEARVCRDVADAVCDGLRQIGDFSYAILPRDLAHALGDFKKAC
                     LSSIRCAVDWEIEWIDERVAGGDRLRDEWYEKCRRTPADETQAGGV"
     gene            843..1700
                     /gene="ispH"
                     /locus_tag="VFO72_08860"
     CDS             843..1700
                     /gene="ispH"
                     /locus_tag="VFO72_08860"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET9479442.1"
                     /translation="MEVLLAEEYGFCFGVERAVEMVEEVVEEGVRPIRSLGPLIHNAQ
                     EMERLGARGVSTIDSPHEADADTIAVIRAHGVTPQVQHELEQRAAKVIDATCPFVTRV
                     QHLAERAARQGRDVVVAGNPDHPEMIGVVGYAPNNTYVVRDAAEVAALPPLRSPLVVS
                     QTTIKLQTFLEVAEAVREKADTEPQVVNTICSATRDRQDAARALAGQVDAFYVIGGRH
                     SSNSVKLLAVCQEQCAKSFLIETPAEINPDDLKGVQKVGVTAGASTPNWLIDQVVERL
                     KNLRPDQAQ"
     gene            complement(1669..2283)
                     /locus_tag="VFO72_08865"
     CDS             complement(1669..2283)
                     /locus_tag="VFO72_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479443.1"
                     /translation="MPSSFMIDDFGGRIATVWSELRPATRGWVERALQAAANGTQASR
                     NFQYDARADLELSRFLAALDDRVAEKSEVLDADTSGKLKSIADTCARMLQEQTESAEV
                     FAQLVRRAEVHKDYGRIDTLADALTSRFPPGEICELARYEDVIVRELANEALARCPVS
                     MLAGLLNDPVDAETARSALRRQVAEYNSEDARRLLTALDQGEDF"
     gene            complement(2519..2743)
                     /locus_tag="VFO72_08870"
     CDS             complement(2519..2743)
                     /locus_tag="VFO72_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479444.1"
                     /translation="MEITKALAALQPLYGKDNVEIITTDGRRVRGTVRSMKSTQALTT
                     PSVKMLCADGTIVKIPFPSIAEIKDHNNLT"
     gene            complement(2834..3013)
                     /locus_tag="VFO72_08875"
     CDS             complement(2834..3013)
                     /locus_tag="VFO72_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479445.1"
                     /translation="MPRRNPQLRQNRKSDGIAEWQFEQVIVGPGDSGISGFCDMTNQR
                     LKAYGRIYDDFLYAK"
     gene            3108..3806
                     /locus_tag="VFO72_08880"
     CDS             3108..3806
                     /locus_tag="VFO72_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9479446.1"
                     /translation="MAWVFVGLLVFFVGAAAFTAIINPIRQNVAVMRTPVVKSWVGVD
                     GWDTTDNGVTFGSVDAPDGPADKAGLVGGDIITKFDGHEIRDEDQMDELMKQTPIGKT
                     VEVEYIRDGEKKTTKLTTISEQEFRQLARNAERRPEGRGVFGYDDNRVERVQVPGTKI
                     YGVKLNAILNSRPADLAGIKNGDVVIEFDGVPIRTPGEFLMRVKRALPYSTVKVVVMR
                     GEEKVEIPVKMGKQ"
     gene            complement(3913..4245)
                     /locus_tag="VFO72_08885"
     CDS             complement(3913..4245)
                     /locus_tag="VFO72_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479447.1"
                     /translation="MNKAGLFMEVVAKYRQFGWELRSALLQPATRSELDTQDIELLKS
                     VPVQESTFDALWFSRPSQNNRDAWELRLLSQNPYALFETFEPNETEDEREEFKQEMEA
                     RMRDYAHK"
     gene            4448..>5281
                     /locus_tag="VFO72_08890"
     CDS             4448..>5281
                     /locus_tag="VFO72_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479448.1"
                     /translation="MSTQQIPVNTIRELKESTEPVPFYEALRKALKKRKANLDLICPP
                     ANPVARRILEEYGAIFVADKKVKPPPVCIFTNEEQVARFQDDAGFKAEYIGYDEVELQ
                     PAALKHLLKAREDAQKEGLDITPRDGAEAGRRNYEDSVRLWNSRFEPALDYWLAQGRL
                     TADEVNRLRSLPLSQQVAEVLELEKSGIYFSKDLSKSILYSIAAPGTSQHIAMLAFDV
                     NEFENPRVREIMAKRGWFQTVLSDLPHFTFLGRKEKDLPKLGLRSVEVNGQVFWIPNV
                     SA"
BASE COUNT         1195 a         1380 c         1525 g         1181 t
ORIGIN      
        1 acaagatcga aaagctgtgg ccgttgatgg cagcggagtc gaaacaggcg cacgacatcc
       61 gcaagtttga agaggtcggc acgcattccg ccgcgccgag attagcaatt ggcgaagcga
      121 tgctgtttca caacggcatg ggcgggaaac gaaaagaagc gcgtttgcgt tacctgtcgc
      181 gctactggat gaatcgtttg aaagacgtcc cgaaggttcg cttcaacacg tcgtttgatc
      241 cgaaccagtc gtgcgcgatc gcaaacgtcg agatcgtcgg gctcgacccg accgcgatag
      301 gcgcctacct cttcaacaag catcgcatct ttaccacgcc aatcctgcac gacgagttca
      361 aaggcatacg catcactccc aacgtttaca cgaccctcgg cgagctcgac cgcttctgca
      421 acgaaatgga gaagatcgcc cgaaaaggcc tgcctaaccc aaccgtgtaa tccgtgaccc
      481 ggaacccaag ctaccgtgcg accgtaaagc agatggtgct agaatctttg gcggaggaag
      541 tatcatgcag gaagcccgag tttgtcgtga cgtggcggat gcggtttgtg acggtttgcg
      601 ccaaattggc gacttttctt acgcaatttt gcccagagac ctggcacatg cgctcggcga
      661 ttttaagaaa gcctgtcttt cgtcgattcg ctgtgcggta gattgggaga tcgagtggat
      721 cgacgaacgc gtggcgggtg gtgaccgcct gagagacgaa tggtacgaga agtgccggcg
      781 cacaccggcc gacgaaaccc aggcgggtgg cgtctaattc cggagagtcg aaaagatcac
      841 ttatggaagt gctactggca gaggaatacg gattctgttt cggcgtcgag cgggcggtcg
      901 aaatggtgga agaggtggtt gaagaaggcg tgcggccgat tcgctcgctt ggaccactga
      961 ttcacaacgc tcaggagatg gaacggctcg gcgcgcgtgg tgtttcgacc atcgattcac
     1021 cgcacgaagc agatgccgac acgatcgctg ttattcgcgc ccacggtgtg actccgcaag
     1081 tgcagcatga attggaacag cgcgctgcga aagtgatcga tgcgacctgc ccgtttgtaa
     1141 ctcgcgtgca gcatctcgcg gaacgcgccg cacgacaggg ccgcgacgtt gtggtcgccg
     1201 gaaatcccga tcatccggag atgatcggcg tagtcggtta cgcgccaaac aatacttacg
     1261 tcgtgcgcga tgccgcggag gttgcagcgc tgccgccgtt gcgttcgccg ttggtggttt
     1321 cacaaacgac gatcaagctg caaacgtttt tggaagttgc cgaagcggtg cgtgagaagg
     1381 ctgacactga accgcaagtt gttaacacga tctgttccgc cactcgcgat cggcaggacg
     1441 cggcgcgcgc acttgccgga caagtggatg cgttctatgt tatcggcgga agacattctt
     1501 caaacagcgt gaagttgctg gccgtgtgcc aggaacagtg tgcgaagagt tttttgattg
     1561 agactccggc ggagatcaat cccgatgatt tgaagggtgt gcagaaggtt ggagttaccg
     1621 cgggagcttc aacgccaaac tggttaatcg atcaggttgt tgagcggctc aaaaatcttc
     1681 gccctgatca agcgcagtaa gtaatcgtcg cgcgtcttct gaattgtact cagctacctg
     1741 tctccgtaac gccgatcgag cggtctcggc gtcaaccgga tcgttcaaca ggcccgccaa
     1801 cattgaaacg gggcaacgcg cgagtgcttc attcgccaat tcacggacaa tcacgtcttc
     1861 ataacgcgcc agctcgcaga tctcgccagg aggaaaacga gacgtgagcg cgtcggccag
     1921 tgtatcgatg cgcccgtagt ctttatgcac ttctgcgcgc cgaacaagct gcgcgaacac
     1981 ttcggctgat tcggtctgtt cctgcaacat gcgcgcgcac gtgtcggcaa tggatttgag
     2041 tttgccactg gtgtccgcgt cgagtacctc gcttttttcg gcgacacgat cgtcgagtgc
     2101 cgcaaggaaa cggctgagtt ccagatcggc ccgcgcatcg tattgaaaat tacgcgacgc
     2161 ctgagttccg tttgctgcgg cctgcagggc gcgttcgacc cacccacgcg ttgccggacg
     2221 cagttcagac cagaccgtgg caatgcgacc gccgaagtcg tcgatcatga aggatgaggg
     2281 catagaacgg cgtactatac gcgatgagat gcgaaaagtg tacttcgctt ggctttgccc
     2341 gaaaagtacc tctatcgcaa gaaatgtctc gcgcaaagac gcaaaggcgc aaagcgctac
     2401 cgcgtttctt aaagggcttt ctttgcgcct ttgcgccttt gcgcgagaat aatttcttcc
     2461 cgtagagatc cgctgattac ctttcgggca agcaacttcg cttcttcgct taaaactttc
     2521 aggtgaggtt gttgtggtct ttgatttccg cgatggatgg aaaaggaatt ttgacgatcg
     2581 tgccgtcggc gcagagcatc ttgacgctgg gtgtcgtcag tgcctgcgtg cttttcatac
     2641 tacgaactgt acctctgact cgccgcccat cagtggtgat gatctctacg ttgtccttac
     2701 cataaagggg ctgtagcgca gcgagcgctt tagttatttc cacgttttgg taatcctccc
     2761 tcgtacatag aatgcgagca cccgctatgc cagcgcaagt gcgccgcagc aaagtggctg
     2821 caactgtcaa agattatttg gcgtatagaa aatcgtcgta tattcttccg taagccttaa
     2881 ggcgctggtt agtcatgtcg cagaatcctg aaataccgct gtcgccggga ccaacaatca
     2941 cctgctcaaa ctgccactcg gcgatgccgt cggacttacg attctgccgt aattgcggat
     3001 ttcgtctcgg caccagcttt ggtaacgcga gccagttcgg cagtactaat atcgccgcga
     3061 ctggaagcgc accgctcgct cagaaaaagc gcaagcggat gagcggaatg gcgtgggtat
     3121 tcgtcggctt gctcgtcttt ttcgtcggcg ctgcggcgtt tactgccatc atcaatccca
     3181 ttcgacaaaa tgtcgccgta atgaggactc cggtagttaa gtcgtgggtt ggcgtcgatg
     3241 gctgggacac gactgacaat ggcgtaacgt ttggttcagt cgatgctccg gacggtccgg
     3301 ctgacaaggc cggtcttgtt ggcggcgaca tcatcacgaa gttcgatggg cacgagatcc
     3361 gcgatgaaga tcagatggac gagttgatga agcaaacgcc gattgggaag actgtcgagg
     3421 tcgagtacat acgcgacggc gagaagaaga cgacgaagtt gacaacgatt tctgagcagg
     3481 agtttaggca gttagcgaga aacgctgagc gccgtccaga gggtcgcggg gtgtttggtt
     3541 acgacgataa tcgcgtagag cgagtccagg tgcccggaac gaagatttac ggcgtgaagc
     3601 tcaatgccat tctgaacagt cgccccgcag acttggccgg gatcaaaaac ggcgacgtcg
     3661 tgatcgagtt cgacggtgtg ccaattcgca cgccgggtga gtttctgatg cgcgtgaaac
     3721 gtgcgcttcc ttacagcacg gtgaaagtgg tcgtgatgcg cggtgaagag aaggtggaaa
     3781 ttccggtgaa gatgggtaaa caatagaggc tcttgaaccc ttgggaccgc atgcgtctcg
     3841 cctgcacgtg tcgaatctgg cgcttctatg ggcttgcagg cgagacgcct gcggtcccag
     3901 ggttaacctg aactatttgt gggcgtagtc acgcatccgc gcctccatct cctgtttgaa
     3961 ttcctcgcgt tcgtcttcgg tttcgttcgg ttcgaaagtc tcaaagagtg cgtagggatt
     4021 ttgtgacaac agccgcagct cccacgcgtc gcggttgttc tgcgatggtc ttgaaaacca
     4081 gagtgcgtcg aacgtcgact cttgaacggg aacgcttttg agcagttcaa tgtcctgagt
     4141 gtccagttcg gatcgcgtag caggttgcag tagcgcgctt cggagttccc acccgaattg
     4201 gcgatacttc gcgaccactt ccataaacaa acctgcctta ttcataaaat ttcgtgaacg
     4261 gagattaaca cacctgggag agaagccctg ttgaccgctt gatctgcccg cacttatcat
     4321 ctccttcgcc ttacggggga gccgtttgag aactcgcttt catttttgcg gcgccaggtt
     4381 catcagtctc gtttttattt tgatctgccg gagcgttgtg ggcgcggcgc caccggcact
     4441 gaatcagatg tcaacacagc agatacctgt taacacaatc cgggagttaa aagagtcaac
     4501 cgagcccgtt cccttctatg aagcactgag gaaggcactc aagaaacgaa aggcaaacct
     4561 cgatctgatt tgtccccctg ccaatcctgt cgcccgccgc atactcgaag aatacggagc
     4621 aatatttgta gcggataaaa aagttaagcc accaccggtt tgcatcttta ccaatgaaga
     4681 gcaggtcgcg aggttccagg acgacgccgg attcaaagcg gaatacattg gttacgacga
     4741 agttgaactc cagcccgcag ccctgaaaca cctgctgaaa gcccgggaag acgcacaaaa
     4801 ggaaggactg gacatcacgc cgcgcgacgg cgctgaggct ggacgtcgga actacgaaga
     4861 cagtgttcgc ttgtggaatt cccgatttga acccgcgctc gattactggc tcgcgcaagg
     4921 ccgtcttact gctgacgagg tcaatcgatt acgaagcctg ccattgtcgc agcaggtcgc
     4981 ggaagttctg gaactcgaaa agtcaggcat ttatttcagt aaagatttgt cgaagtcgat
     5041 cctttattcg attgcagctc cgggcacatc gcaacacatc gcgatgcttg cctttgacgt
     5101 gaacgaattt gagaacccgc gtgtgcgcga gatcatggcg aagcgtggtt ggttccaaac
     5161 cgtgctgagc gacttgccgc acttcacttt tctcggccgg aaagaaaaag atttacctaa
     5221 gctcggtttg cgcagcgtcg aagtaaatgg gcaggtgttt tggataccga acgtatcggc
     5281 c
//
LOCUS       DASTEY010000509         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1131871, whole genome shotgun sequence.
ACCESSION   DASTEY010000509 DASTEY010000000
VERSION     DASTEY010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131871"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFO72_08895"
     CDS             <1..324
                     /locus_tag="VFO72_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET9479449.1"
                     /translation="DLYSREGAVNIEGYEMLKGLQGVRTHEHHEWLPIIKNSQDIPAL
                     ANTVGDTLNNYSAIHGFLLERHGLYSWGDDLAQAKRHIEILEFLLETVGRTLLAKNRG
                     LKVWP"
     gene            315..884
                     /locus_tag="VFO72_08900"
     CDS             315..884
                     /locus_tag="VFO72_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9479450.1"
                     /translation="MAIVTIADENRTVRTEPEITSLLAGHGIDYERWNPAHPVSDNAP
                     AEEILRAYAREIEALKQRGGYVTADVIDVDPNTAGLEAMLAKFSREHWHDEDEVRFII
                     AGHGLFHIHTTAGPVLAIEVEAGDLIRVPRGTLHWFTLCADRRIRAIRLFQDTSGWTP
                     HYTDSRLDEQFLPVCFGLSHISHQRVTTS"
     gene            881..1612
                     /gene="mtnC"
                     /locus_tag="VFO72_08905"
     CDS             881..1612
                     /gene="mtnC"
                     /locus_tag="VFO72_08905"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HET9479451.1"
                     /translation="MSATVSITDARGILLDIEGTTTPIAFVHETLFSYAREHVRDYLA
                     KHFAAEDVRADIQLLREEHAVDVNEGKQPPPLTAETESIVAYVNWLIDLNRKSTGLKS
                     LQGKIWQEGYSVGTLRAPVFPDVAPALVRWRNRGLSVSIFSSGSVLAQQLLFRHTEAG
                     DLTPFINNYFDTNTGKKSEAESYRHIAQAIGLAPDEILFVSDVVSELAAAGEAGMKTV
                     LSIRPGNQSQEHADRYQSIHSFQEL"
     gene            complement(1910..2875)
                     /locus_tag="VFO72_08910"
     CDS             complement(1910..2875)
                     /locus_tag="VFO72_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479452.1"
                     /translation="MAQDITGGGSLVRDITGGAALIFRTPQNPSIHLTSGGAQGPSGG
                     RIRNRTPKPSVPKQDSIIARANAARSAPSPRYAEAEQQYQLAAQIAPDDARAFAGLGN
                     VYVDQGKFAEAVNAYQQALKVKPDYSAAYLPLAFSLARLNRYPEAIDVYQQTLKFDPT
                     SPEVHNNLSFAYNHTGRYQEAVDASQQAIELLGETGEAFKLGFQERNEIRSYAYKNLG
                     NAYSGLKRYDEAANALKKAAEIEPKNASAHFNLGLTLYTAGRYSEAIESYKEVIKLRP
                     QLAQAYFNLALTYIAINDKPQAMVQYETLKGLDTEMAKQLYEAIK"
     gene            complement(2972..>3262)
                     /locus_tag="VFO72_08915"
     CDS             complement(2972..>3262)
                     /locus_tag="VFO72_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479453.1"
                     /translation="EPDRTLRLNALTDMGSVSDLSKLTAKLIAVFQERTRNKVYRADM
                     LDRIDLPEQLRIEKTVFIKAPRSMRYGEVMQVMDGLKGAGAEPIGLQLDDLN"
BASE COUNT          726 a          925 c          878 g          733 t
ORIGIN      
        1 gatctctatt cgcgagaagg cgctgtcaac atcgaaggct acgagatgtt gaaaggattg
       61 cagggcgtgc gcacacacga acatcacgag tggctgccga tcatcaaaaa ctcacaggac
      121 atcccggcac ttgcgaacac cgtcggcgac acgctaaaca actacagcgc cattcacggt
      181 ttcctgctcg aacgtcacgg actctattcc tggggcgacg acctggcgca ggcaaaacga
      241 catatcgaga ttctcgagtt tctactcgaa acagttgggc gaacattgct cgcaaaaaat
      301 agagggttga aggtatggcc atagtaacga tcgctgatga aaacaggacc gtccgcactg
      361 aaccggagat cacttccctg cttgccggcc acggcatcga ttacgagcgt tggaaccctg
      421 cgcatccagt ttcggacaac gcgcccgccg aagagatcct gcgcgcatac gcacgcgaga
      481 tcgaagcgct gaagcaacgt ggcggttacg ttaccgccga cgtcatcgac gtcgatccaa
      541 acaccgccgg tctcgaagcg atgctggcga agttcagccg ggaacactgg cacgacgaag
      601 acgaagtgcg tttcatcatt gccggccatg gactgtttca tattcacacc accgccggac
      661 ccgtgctggc gatcgaggtc gaggccggag acctgattcg cgtaccgcgc ggcacgttgc
      721 actggtttac tctctgcgcc gatcgacgta ttcgcgccat ccggctgttt caggacacgt
      781 ccggctggac gccgcactac actgacagcc gtctcgatga acagtttctg ccagtctgtt
      841 ttggactttc acacatctca caccagcgcg tgacgacgtc gtgagtgcaa ctgtttccat
      901 taccgacgcc cgcggcatcc tcctcgacat cgaggggacg accacgccga tcgcgtttgt
      961 tcacgaaaca ctcttctctt acgcgcgaga gcacgtgaga gactatctcg caaaacattt
     1021 cgctgctgaa gacgtgcgcg ccgacattca actcttacgc gaagaacacg cggtcgatgt
     1081 gaacgaaggt aaacaacccc caccgttgac ggcggaaacg gaatcgatcg tggcctacgt
     1141 aaactggctc atcgatctca accggaaatc caccggactg aaatcactgc aaggaaagat
     1201 ctggcaggaa ggttattccg tcggcacgtt gcgcgctccg gtctttccgg atgttgctcc
     1261 cgcgctggtt cgctggcgga atcgcggctt gagtgtcagt atcttctcgt cgggcagtgt
     1321 cttggcccag caacttctct tccgccacac tgaagcgggc gatctcacac cattcatcaa
     1381 caactacttc gacaccaaca ccggtaagaa gagcgaagcc gaaagctatc gtcacattgc
     1441 gcaagctatc ggactcgcgc cggacgagat actttttgtc tccgacgttg tgagtgagct
     1501 tgcggccgca ggtgaggccg gcatgaaaac cgtgctttca attagaccag gtaatcaatc
     1561 acaggaacac gcagatcgat accaaagcat tcactctttt caggagcttt gaagtttgcc
     1621 acgcagcaag cgtttcgacg aagcacggcg agacaccgag gctttgcaca aaaaccacgc
     1681 cgaaggcgct cgcaaggtct aagcccaggg tttataccct gggaacaaag aatttcactc
     1741 cgcccgcaga actgtgtaag agttcgccaa ctccttcgga gttgtgtagt taagaggacg
     1801 atgttttccc agggtataag ccctgggctg caatttgcga actccttcgg agttcagtcg
     1861 aagcagctta ccgacttttt ttgcaaagcc aggcacccga acgaaatgac tacttgatgg
     1921 cctcgtatag ttgttttgcc atctcggtgt cgagaccttt gagggtctcg tactggacca
     1981 tcgcttgcgg cttgtcgttt atcgctatgt aggtaagagc gagattgaag taggcttggg
     2041 ccaactgtgg tcgcagctta attacttcct tgtacgattc gattgcttcg gagtagcgac
     2101 ctgcggtgta aagtgtcaac ccgaggttga aatgcgccga tgcgttcttc ggttcgatct
     2161 cggccgcctt cttcagcgcg ttcgcggctt cgtcataacg cttcaagccg ctgtaagcat
     2221 tgcccaggtt cttgtaagcg taagatcgaa tctcatttcg ttcctggaac ccaagcttaa
     2281 aagcttcgcc ggtttctccc agtaactcga tcgcctgctg gctcgcatcc accgcttcct
     2341 gatagcgtcc ggtgtggttg tacgcgaaac tcagattgtt atggacctcc ggactggtcg
     2401 gatcgaactt caacgtctgt tggtaaacgt cgattgcctc cgggtaccgg ttcaaccgcg
     2461 ccagcgaaaa tgccaacggt aaataagctg cggaatagtc cggtttcact ttcagcgcct
     2521 gctggtaagc gttgacggct tcggcaaact tgccctggtc cacgtaaacg ttacctaaac
     2581 cggcaaaggc acgcgcatcg tcaggcgcga tctgtgccgc gagttgatat tgttgttccg
     2641 cttccgcata acgcgggctg ggagcactgc gagcggcatt cgcgcgggcg atgatcgaat
     2701 cctgtttggg aacagacggc ttcggtgtgc ggtttctgat gcgtccgcca ctgggacctt
     2761 gtgcgcctcc ggaagtaagg tgaattgatg ggttctgggg ggtgcgaaag atcagtgcgg
     2821 cgccgccggt aatgtcgcgc accaggctgc caccgccagt gatgtcctga gccatcacac
     2881 tcgcggcggt aagaccaata accaaagaaa tcaggggaaa aagccggttt gtcatgctta
     2941 agtgcatctc ctcaccggca gattacaact tttagttcag atcgtcgagt tgcaggccga
     3001 ttggctcggc accggcgccc ttcagcccgt ccatcacctg catcacctct ccgtaacgca
     3061 tgctgcgtgg ggcttttatg aagactgtct tctcgattcg caactgctca ggtagatcga
     3121 tccggtccag catgtccgct cgataaactt tgttccgcgt gcgttcttga aaaactgcga
     3181 tcagcttcgc cgtcagcttt gaaagatcgc ttaccgatcc catgtcggtg agcgcgttga
     3241 gccgcaaggt acgatccggt tc
//
LOCUS       DASTEY010000510         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_472848, whole genome shotgun sequence.
ACCESSION   DASTEY010000510 DASTEY010000000
VERSION     DASTEY010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472848"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFO72_08920"
     CDS             complement(<1..1194)
                     /locus_tag="VFO72_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9479454.1"
                     /translation="MNKKLIAVAAFVLIASAAALFLVASPNSATNSSAGPFVRQRGGR
                     FFVGNRPFRFVGANVAVMYRDDDRAGMPETLRQAAQAGIKVVRVWAFGEGGPNDVKPM
                     TDFADWPRHHSFRFAPGEWNEDAFVHLDKVIAEAAKNKIYVQLCLTNWWRDTGGVTQY
                     LRWAGINDAADDRYKFGINNERAILFYSNPETRRLYREHVEKLATRRNTITGMLYRDD
                     PAIFGWELMNEAQAITGRWAERRAWFAEMSAYLKSLDPNHLIAPGAWGYRSSSERREW
                     LADHAIPTIDYCDVHNYPRDDHDSFVDSPGALKEFIENRAAAAFSLGKPLVLGEFGMN
                     AEGHNGESQAEWYRAFFEGSARAGAGGAMFWILTPDPQRGYGVTYATPRDKDVLAEVS
                     RASQMF"
     gene            complement(1220..1582)
                     /locus_tag="VFO72_08925"
     CDS             complement(1220..1582)
                     /locus_tag="VFO72_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479455.1"
                     /translation="MFFPTLMLLQQLRATQPNYFFLVTLVLLIAGGVGWLIAAVLGFA
                     RSPAFGPSARWFSYAAVCLIIYHLHFLLFGVFVFLGMSQNSVDLTMALGVGAFFNLFV
                     VGGAVCAIMGFVRMTSPR"
     gene            complement(1989..2207)
                     /locus_tag="VFO72_08930"
     CDS             complement(1989..2207)
                     /locus_tag="VFO72_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479456.1"
                     /translation="MKSLKDILAIVVGILAAIGAIYYFYRFVTDPNPAGGQSLGWIAL
                     GLAAIAFVCGLVYFLGHVNKEEEIHITQ"
     gene            complement(2315..>3337)
                     /locus_tag="VFO72_08935"
     CDS             complement(2315..>3337)
                     /locus_tag="VFO72_08935"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HET9479457.1"
                     /translation="LGRVTVLDGPAEIPAGGGALGQVRLEAPVVALHDDRFILRSYSP
                     SDTIGGGRVVNTFAPKHRRKDVSDAVQLLRSMMNAERATKFAGFVRTAEKHGARLSDL
                     AAATGWTNEVLAQAARDASTESSVIEAGGVYLSSESFALLSSNVVAELEQHHKREPLA
                     RGMLRETLREKLFAHSLPELFGAVIGALEASGEVVSEKDVVRASRHRVDLSDKDAELS
                     AKIEKLYLDAGVEAPSLNEAMTRAGVAAAQRAQARKILQLLLDNRKLVRIQGEMFMHA
                     EVIESLKTKLQTYARQHEPDRLIDVPTFKTLAGVSRKYAIPLLEYFDRIPVTRRAGDK
                     RLILKI"
BASE COUNT          809 a         1006 c          832 g          690 t
ORIGIN      
        1 aaacatctgc gaagctcgtg acacctcagc cagcacgtct ttatcacggg gcgtcgcata
       61 cgtcacgcca tagccgcgct gcggatccgg ggttagtatc caaaacatcg cgccaccagc
      121 acccgcgcgt gcactgcctt cgaaaaacgc ccggtaccac tcagcctgcg actcgccgtt
      181 gtgaccctca gcattcatcc caaactcgcc caacaccagc ggtttgccaa gtgaaaacgc
      241 agccgctgca cgattctcga tgaactcctt caatgcccca ggcgaatcta caaaactgtc
      301 gtgatcgtct cgcggatagt tgtgtacatc gcaatagtcg atcgtcggta tcgcgtgatc
      361 cgccaaccat tcgcgacgtt cagacgaaga acgataaccc caggcgcccg gcgcaatcag
      421 atgattcgga tccagagact tcaaatacgc gctcatctcc gcgaaccacg cgcgccgctc
      481 ggcccaacgg cccgtaatcg cctgcgcctc attcatcaac tcccacccaa agatcgccgg
      541 atcgtcgcga taaagcatgc cggtgatcgt gtttcgacgt gtcgccagtt tttcgacgtg
      601 ttcgcggtac agccgtcgcg tttccggatt tgagtaaaac agaatggctc tttcattgtt
      661 gatgccaaac ttgtaacggt cgtccgcagc gtcgttaatt cccgcccagc gcaagtattg
      721 cgtcacaccg cccgtatcgc gccaccagtt ggtcaagcag agttgcacgt aaattttgtt
      781 cttcgccgct tcggcaatga ctttgtcgag gtgtacaaac gcatcttcgt tccactctcc
      841 cggagcgaac cgaaacgagt gatgccgcgg ccagtcggca aagtcggtca tgggtttaac
      901 atcgttcgga ccaccttcac cgaatgccca aacacgcacc accttgattc cggcctgcgc
      961 tgcctggcgc agcgtttccg gcattcccgc gcgatcgtcg tcgcgataca tcacggccac
     1021 gttcgcaccg acaaagcgaa acggacgatt gccgacaaaa aaccggccgc cgcgctgacg
     1081 cacaaacggt ccggcagacg agttcgtcgc agaattgggc gaagccacca aaaaaagagc
     1141 cgccgcgctt gcaatcagca caaacgcggc gaccgcaatc agtttcttat tcatcaggac
     1201 caagttaatc ggggcgattc tatctcggac ttgtcatccg gacaaaaccc ataatcgcac
     1261 acaccgcacc accaacaaca aatagattga aaaacgctcc gacgcccaac gccatcgtga
     1321 gatccaccga gttttgactc attccgagaa acacaaacac tccgaaaagc agaaagtgca
     1381 ggtgataaat aatcaggcag acggctgcgt aagaaaacca gcgcgcggaa ggtccaaacg
     1441 cgggcgaccg cgcgaaacca agtacggcag cgatcaacca gccaacaccg ccggcaatca
     1501 acaataccaa cgtgaccagg aagaagtagt tgggttgtgt cgcccgaagc tgctgcaaaa
     1561 gcatcagggt tgggaagaac atcggtatcg actctctcct tttcggcgag gcgccagctt
     1621 tgcctcaaaa gtcctctctt attctcaccg ggctttaagc ccagtgcagg aacagtgaag
     1681 acggaggaaa ccgtttcaac ggttttcggt ggattcgcca gcgaaaccgt tgaaaacggt
     1741 ttctcacaat cgcgtgccct ttcaccgggc tgaagcccgg tgagaatgaa agaagacttt
     1801 tgagacaaag ctgcgccaat tcgtaaaact aaacaaaaac agttcacaaa aagcgagcgc
     1861 cgtttgcgca cttgagccgc tttggtatgt gcacaaacgg cgtccaattg gtgtcggtga
     1921 ccaagcgcaa gttcttacag caggaagtca gcagtggctc ccaaccattg gtctcggctg
     1981 gcgctcgcct attgagtaat gtggatctcc tcttctttgt taacgtggcc gagaaaatag
     2041 accaggccac aaacaaaggc tattgccgcc agcccgagtg cgatccatcc caaactttgt
     2101 ccaccggcag gattcggatc ggtcacaaac ctgtagaagt agtagatcgc cccgatagct
     2161 gccagaatgc ccacgacaat cgcaagtatg tctttcaggc ttttcatttt gaacctccgg
     2221 ccaggtaatt atttatgaag gttaaggaaa aagccgccaa attgcgctaa atgatgtctg
     2281 catgttaacg ccgtgaagca ggtgaatgca agcctcagat tttgagaatt agtcgcttgt
     2341 caccggcacg gcgagtgact ggtatgcggt cgaaatattc gagcaacggg atagcgtact
     2401 tccgtgaaac gccggccagc gttttgaaag tgggaacgtc gatgaggcga tcgggttcat
     2461 gttgccgggc gtaagtttgc agcttggttt tcagactttc aatcacttct gcgtgcatga
     2521 acatctcgcc ctgaatgcgc acgagcttac ggttgtcgag cagtagttgc agaatcttgc
     2581 gcgcctgcgc acgttgcgct gctgcaactc ccgcgcgcgt catcgcttcg ttgagagacg
     2641 gagcttcaac gccggcgtcg agatacagtt tttcgatctt cgcgcttaac tccgcatcct
     2701 tatcagacaa atcaacgcga tggcgactcg cccgcacgac gtccttttct gagacaacct
     2761 cgccgctcgc ttccaacgcg ccaatcacag cgccaaacaa ctccggcaac gaatgcgcaa
     2821 acagtttctc acgcagcgtc tcacgcaaca tgcctcgcgc taacggttcg cgtttgtgat
     2881 gttgttccag ttcagcaaca acattgctac tcagtagtgc gaagctctcg ctcgataggt
     2941 aaacgccgcc ggcttcgatt accgacgact ccgtcgacgc gtcgcgagcc gcctgcgcca
     3001 gaacttcatt ggtccaaccg gttgccgcag ccaggtcact caacctcgcg ccgtgtttct
     3061 cagcagtacg aacgaaaccg gcaaactttg tcgcccgctc cgcgttcatc atcgagcgca
     3121 agagttgcac tgcgtctgac acgtctttgc ggcgatgctt gggcgcaaat gtattcacta
     3181 ctctgccacc tccgatcgtg tctgacggcg agtagctacg caaaataaag cgatcgtcgt
     3241 gcaacgcaac aacaggagct tcgagtctga cttgccccag cgcgccgcca cccgccggaa
     3301 tctccgctgg gccatcgagc acggtgactc ttcccaa
//
LOCUS       DASTEY010000511         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1164490, whole genome shotgun sequence.
ACCESSION   DASTEY010000511 DASTEY010000000
VERSION     DASTEY010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164490"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFO72_08940"
     CDS             complement(<1..500)
                     /locus_tag="VFO72_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479458.1"
                     /translation="MSLKLTTSLSQRLVLTPQLRQRIEMLQMTSLELSDLIQQQLLEN
                     PVLEEVPTQEEVREIAEKVLDHLASADPASSFDEPGGGNSEPVNVSTNGSGDADVVAS
                     LPQAGEAEGGEAVAAAEQVDDGVADDGGVDEQRDAFEEIDFGREFQDYLDPGYKTQEI
                     EYKEDAP"
     gene            complement(605..1468)
                     /gene="lptB"
                     /locus_tag="VFO72_08945"
     CDS             complement(605..1468)
                     /gene="lptB"
                     /locus_tag="VFO72_08945"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET9479459.1"
                     /translation="MNSFPEQRIALLGPQASRLQIVEGGRLREKAGETPAVPGERASA
                     PAVESALRTINLVKHYGKRRVVDDVSLQVQKGEVVGLLGANGAGKTTTFYMIVGLERP
                     DSGNVLLGDQELTKLPMYLRARLGIGYLPQEPSVFRKMTAAQNILAVLETVGLRRREQ
                     LKRLEELLEEFGIADVRNTRGDALSGGERRRTEIARALATEPQYILLDEPFAGIDPKA
                     VDDIQNVILYLRDRGIGILITDHNVRETLGVTDRAYIMAEGRIFRSGQPQDLTEDAEV
                     RRLYLGEKFRM"
     gene            complement(1465..>2604)
                     /locus_tag="VFO72_08950"
     CDS             complement(1465..>2604)
                     /locus_tag="VFO72_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET9479460.1"
                     /translation="AEASGNAELFIEPVTSSARAERRKLNAPQFHCDFFEAGNLVRTC
                     KAAGGAKAVMDPLQPTPKRGTRTFTSQTMTAVFVRETQDIERVDAHGEGKFNENDRNG
                     VAANVSYAAADETVRLRGGDPTVWDSRCRTKAVELDSDLANNVSYARGRTATTYYSQG
                     QTNGATPFSKTKSPVYIASDKGEFRHESGQGIYTGNARAWQDDNYVRGDKLIVYVNDK
                     RMEAVGHVQTAVYNSRRRVDNNVAVVPVFASADSMFYSDPDRTIHYEGNVDIKQGTDR
                     MTSGVADVYLARDSNEMEKTISQRNVVLTQPNRRGTGDWVEYTAATEIAVLKGNPARV
                     DDVEQGNTEGARLILSIRDSKVTADDARGPLSPGRVRSTHKIRKP"
BASE COUNT          490 a          810 c          701 g          603 t
ORIGIN      
        1 ggcgcgtctt ccttgtactc gatctcctgt gttttatagc cgggatcgag atagtcctga
       61 aactcgcgtc caaaatcgat ctcttcaaaa gcgtcacgct gctcatcgac gccaccatca
      121 tcggccacac catcgtcaac ctgttcggcg gcggcaaccg cttcgccgcc ttccgcctcg
      181 cctgcttgag ggagactcgc gacaacatcc gcgtcgccgc tgccgttggt tgaaacgttt
      241 accggctcac tattgccacc gcccggttcg tcgaacgaac tagccggatc agcgctggct
      301 aaatggtcga gcaccttctc cgcgatctcg cggacctctt cctgtgtagg gacctcctcc
      361 aggaccggat tctcgagcaa ctgctgctga atcagatcgc tcaactcgag cgacgtcatc
      421 tgcaacatct cgattcgctg gcgcaactgc ggcgtcagta cgaggcgttg agataggctg
      481 gtggttaatt taagtgacat gctttcgagc gctgattata accgattcag tgtcatatgt
      541 ggcagaggtc tgcccccaaa ggtcataaac gcaacaaagt ttacacgaag atctaaacac
      601 tctcttacat gcggaatttt tcgcccagat aaagccgcct cacctcggca tcttccgtca
      661 gatcctgcgg ctggcccgac ctaaaaattc gcccttccgc cattatgtag gcgcggtccg
      721 tgacgccgag tgtctccctc acgttgtggt cggttatgag gattccgata ccgcggtcac
      781 gcaggtagag tattacattc tgaatatcgt cgacggcctt cggatcaatt cccgcgaagg
      841 gctcgtcaag tagaatatac tgcggttcgg tcgcgagtgc gcgagcaatt tcagtccgcc
      901 gcctttcacc gcccgataat gcatcgccgc gagtgttgcg cacgtcggcg attccaaact
      961 cctccagcag ctcttccagc cgcttcaact gttcgcgccg gcgcagtccg actgtctcca
     1021 gcactgcgag aatattctgc gccgccgtca tcttgcgaaa caccgaaggc tcctgcggca
     1081 aatatccgat tcccagtcgc gcgcgcagat acatcggaag ttttgttaat tcctgatcgc
     1141 cgagcagaac gtttcccgaa tcggggcgtt cgagtccgac gatcatgtaa aaggtcgtcg
     1201 tcttacccgc accattcgcg ccgagcagtc ccaccacttc gcccttttgc acctgcaggc
     1261 ttacgtcgtc gacgacgcgg cgcttaccgt agtgtttgac cagattgatg gtccgaagtg
     1321 cggactctac cgctggggct gaggctctct ctcctgggac cgcaggcgtc tcgcctgcct
     1381 tctctcgcag acgaccccct tcgacgatct gcaggcgaga cgcctgcggt cccaggagag
     1441 cgatccgctg ttccggaaac gagttcacgg ttttctgatc ttgtgcgtag atcgcacgcg
     1501 gccaggcgag agtggtccac gcgcatcgtc tgcggtgact ttactgtcgc ggatcgaaag
     1561 gatcaaacga gcgccttcag tattgccctg ctccacgtcg tctacgcgtg cggggttgcc
     1621 cttcaacact gcgatctcag tggcagccgt atattcaacc caatcgcccg ttccgcgtct
     1681 gttcggctgc gtaagcacaa cgttgcgctg cgaaatcgtt ttctccatct cgtttgaatc
     1741 cctggcgaga tagacgtccg cgacaccgct cgtcatccga tccgtgccct gtttgatatc
     1801 gacgttgcct tcgtagtgaa tggtccgatc gggatccgag tagaacatcg aatccgccga
     1861 agcaaacacc ggcacaaccg cgacgttgtt atcgacgcgc cgcctggaat tgtaaacggc
     1921 cgtctgcacg tggcccactg cttccatacg tttgtcgttg acgtaaacga tcagcttgtc
     1981 gccgcgcacg taattatcgt cctgccacgc gcgcgcattg ccggtgtaaa ttccctggcc
     2041 gctttcatgc cgaaactcgc ccttgtcgct ggcgatgtag acgggactct tcgtcttcga
     2101 aaacggcgtg gcgccgttgg tctgtccctg gctgtagtac gttgtcgcag tgcgtccgcg
     2161 agcataggag acgttgttcg cgagatctga atcgagttcg acagccttgg tgcggcagcg
     2221 tgaatcccag accgtcggat cgccgccgcg caatcgcacg gtttcatcgg cagcggcgta
     2281 cgagacgtta gccgcaacgc cgttccgatc gttctcattg aacttgccct cgccgtgcgc
     2341 atcgacgcgt tcgatgtcct gcgtctcacg cacaaagacg gccgtcatgg tttgcgacgt
     2401 gaacgtgcgc gtgccgcgtt ttggcgtggg ttgcagagga tccatcacgg ctttcgcgcc
     2461 gccggcagct ttgcaggtcc gcacgagatt gccggcttca aaaaaatcgc aatggaattg
     2521 cggcgcgttc agtttgcgcc gctcggcgcg ggcgctgcta gtgaccggtt caataaacaa
     2581 ctcagcattt cccgaagctt ctgc
//
LOCUS       DASTEY010000512         6824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1082965, whole genome shotgun sequence.
ACCESSION   DASTEY010000512 DASTEY010000000
VERSION     DASTEY010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082965"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..750)
                     /locus_tag="VFO72_08955"
     CDS             complement(34..750)
                     /locus_tag="VFO72_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9479461.1"
                     /translation="MTALLAAHEVSKIYQMGTNSVTALDNVSLEIREGEFVAIQGTSG
                     SGKSTLLNMLGGLDHPTRGEVYFASQPLGPFTKKEMARYRRFSVGMIFQNFNLIPTMT
                     ARENVSLALAFGGLRGQQRRARSSELLARVGLGDRLSHKPSELSGGEQQRVAIARALA
                     NNPKVLLADEPTGNLDSIRAHELLALLREMVNQDALTVLMVTHDHELATSFADRIVFM
                     KDGKIINGGSHGPTAHADDA"
     gene            complement(750..2618)
                     /locus_tag="VFO72_08960"
     CDS             complement(750..2618)
                     /locus_tag="VFO72_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9479462.1"
                     /translation="MSFRELHKRWEFDLKSSPERLWPFIADTNRFNRDTGVPEIEVDA
                     AHKPLRNARRKVRLSLYGVPVEWEEQPFEWVKPFRFGIERIYHKGPLARLRVLAELTP
                     NTSGGTHLVYQLWSTPRNLPGAVAISLQIKLIVARRFREAIQKYDTAASEGLAIETSE
                     PNTSQSLFDLARLTSLHQKLAADLEQAQLPAEKRAVADRLADFLEHADDLAVTRIRAY
                     KLADDWNQPRRDVLEICLRATRVGLLDFQWDLLCPLCRGPQESGQSLRDINAHVHCEA
                     CKIDFTVDFDRYVELTFRPNPAVRRVAVTSFCVGSPEFTPHVVVQQLLPPGERRSLNL
                     PLEIGSYRLRALELPGSQDVTVSSGGATSVQVAVAQNGWRHDPLQVAERFTLDLQNET
                     GAEQLLILERMAWNDQATTAAEVTALQMFRDLFAAEALRPGEQISVGTLTVLFTDLRD
                     STKLYREIGDATAFGRVMNHFDVLKKAIAEHDGALVKTIGDAVMAVFRRASDGLVAML
                     EAQRVLAQPPEGTMPLQLKAGLHTGPCIAVTLNDRLDYFGSTVNLAARLEGLSTGSDV
                     IISRSVYEDTKVRELLEAQRLAASEFDMSLKGFENERFELWRISERPRKDTEDTDN"
     gene            complement(2732..3253)
                     /locus_tag="VFO72_08965"
     CDS             complement(2732..3253)
                     /locus_tag="VFO72_08965"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET9479463.1"
                     /translation="MSIISDVIESTKAIIQGMGVTLSYIPKRKWTVEYPDEPVTVQPR
                     YRGQHLLHVDELGREKCVACYLCAAACPSECIYIVAADDPRPPEERIGVDERYAAVYN
                     IDYGRCIFCGYCVEACPKDAITHGYNFELSVYSRADLLKTKDDLLITKQLQSKNYRVA
                     LSHEDVDSEYKEV"
     gene            complement(3274..3807)
                     /locus_tag="VFO72_08970"
     CDS             complement(3274..3807)
                     /locus_tag="VFO72_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET9479464.1"
                     /translation="MKYCSQCNRQYTEPWITFCSDDGSLLIEELSPPADPNWNPSIRE
                     PKVKPPASERQTQWLPRDPPAPGGWVAPDERPPMQPVWQPPPPPPRPIKQQSQGLATA
                     SMIIGIIGLLFGCFGPLTGVLAVVLGWLALAQIKKTPDTAGGKPMAIIGIVTGAATII
                     FYGLLFLLFFGGVFGNL"
     gene            complement(3829..4245)
                     /gene="ndk"
                     /locus_tag="VFO72_08975"
     CDS             complement(3829..4245)
                     /gene="ndk"
                     /locus_tag="VFO72_08975"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET9479465.1"
                     /translation="MTTLTFGIIKPDAVRAGKTGSILQRILEDGFKIRGLKLIHMTRK
                     QAEGFYAVHRERPFFSGLTEFMSSGPCVVMALEKENAVKAWRDLMGATDPAKADAGTL
                     RKEFGGSVGENAVHGSDSDENAAIEIAYFFSRLELV"
     gene            complement(4261..5127)
                     /gene="sucD"
                     /locus_tag="VFO72_08980"
     CDS             complement(4261..5127)
                     /gene="sucD"
                     /locus_tag="VFO72_08980"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET9479466.1"
                     /translation="MSVLVDKSTRLIVQGITGKEGTFHTKQMVEYGTNVVGGVTPGKG
                     GTTHEGIPVFNSVVDAVREAGANASVIYVPPSFAADAIMEAADAGIAVVVCITEGIPT
                     LDMVKVKEYLSDRQTRLIGPNCPGIISPGKCKIGIMPGHIHREGNIGVVSRSGTLTYE
                     AVGQLTALGLGQSSAIGIGGDPIVGTTHVDAFRLFEADPETIGIVMIGEIGGTAEEDA
                     AAFAKDNVSKPIVAFIAGQTAPPGRRMGHAGAIIAGGKGTAAEKMKALDEAGIHVVKS
                     PADIGKAMKEAL"
     gene            complement(5209..6615)
                     /locus_tag="VFO72_08985"
     CDS             complement(5209..6615)
                     /locus_tag="VFO72_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9479467.1"
                     /translation="MASISFWGGVGTVTGSKYLIETDKARVLVDCGLFQGLKELRERN
                     WQEPPFDPRSLNAVLITHAHIDHTGYLPRLVKQGFHGPVYCSRGTADLLKILLPDSGR
                     LQEEDADFRNRHKLTVHDPALPLYTEDDAYAAVDLIRPVANEGDGIVPAPGIHVSFKT
                     AGHILGSSLVLAEVQGAGSDGRARRILFSGDLGHYDQPIIRDPSPPPPCDYLLVESTY
                     GDRLHDPEDPKVALARIINDAAQRDAPVLIPAFAVGRTQEIVYLIRELEDEKAIPVLP
                     VVVDSPMAAAATAAYANRKEEHDEEYASILMQKRHPLRTHSMVNATSRQESKRVNDAK
                     GARVIISASGMMTGGRVLHHALRMVPDPEATIVFVGFQAAGTTGRRILDGEPEVKILK
                     QWIPVRCRLAKIGGFSAHADWKEVLQWLEGMETGVPSRTFLTHGEPEAANAMAGHIKD
                     RFGWNVHVPQYGERFELV"
BASE COUNT         1445 a         2009 c         1839 g         1531 t
ORIGIN      
        1 agcgaagcga agagcggcac ggagcgaagc gcttcaagcg tcatctgcgt gggctgttgg
       61 gccatgggat cctccattta tgatctttcc atctttcatg aacacaattc gatcggcaaa
      121 gctggtggcg agttcgtgat cgtgcgtgac catcagcacc gtcaacgcgt cctggttgac
      181 catctcgcgc agcaacgcga gcagttcgtg cgcgcgaatg gaatcgagat ttccggtcgg
      241 ctcgtcggcc agcaggacct tcggattgtt tgccagcgcg cgagcaattg cgactcgctg
      301 ctgttcgccg ccagacagct ccgaaggttt gtgcgacaaa cgatcgccga gtccgactct
      361 cgccagcaat tcactcgaac gcgcgcgccg ctgttgtccg cgcagcccgc caaacgcaag
      421 cgccagcgac acgttctcgc gcgcggtcat cgttggaatc aaattaaagt tctgaaagat
      481 catgccgacc gaaaaacgcc ggtaacgcgc catctctttc ttcgtgaacg ggcccagcgg
      541 ctgcgacgca aagtagactt cgcccctggt cgggtgatcg agtccgccaa gcatgttgag
      601 caacgtggat ttgcccgagc ccgaagtgcc ttgaatggcc acgaactcgc cttcacgaat
      661 ttcaagcgag acgttatcga gcgccgtcac gctgtttgtg cccatctggt aaatttttga
      721 aacttcgtgc gcagctaaaa gagcggtcat caattatctg tgtcttccgt gtctttccgt
      781 ggccgttcac tgattcgcca caactcgaat cgttcgttct caaatccttt caatgacatg
      841 tcaaactccg acgctgccag acgctgcgct tcgagcaact cgcgcacctt tgtgtcttcg
      901 taaaccgagc gcgagattat cacgtcgctt cccgtcgaga gtccttcgag ccgcgccgcg
      961 agatttaccg tagagccgaa atagtcgagg cgatcgttca gtgttacggc gatgcatgga
     1021 ccggtgtgca aacccgcttt caactgcaac ggcatggttc cctccggcgg ctgggccaac
     1081 acgcgttgtg cttcgagcat ggccaccaaa ccgtccgaag cacgacgaaa aacagccatg
     1141 acggcgtcgc cgatcgtctt cacgagcgcg ccgtcgtgtt cggcgatcgc tttcttcaac
     1201 acgtcgaagt gattcatcac gcgcccgaac gccgttgcgt cgccaatctc gcggtaaagc
     1261 ttcgtcgaat cgcgcagatc cgtgaacaac acagtcaacg tgccgacgga gatctgttcg
     1321 cccggtcgca acgcctcggc ggcaaacaga tcccgaaaca tttgcaaggc cgtcacttca
     1381 gccgcagtcg tcgcctgatc gttccaggcc atacgctcga ggatgagcaa ctgttcggcg
     1441 ccggtctcgt tttgcagatc gagtgtgaac ctttcagcta cttgcaacgg gtcgtggcgc
     1501 caaccatttt gtgcgaccgc gacctgcact gacgtcgcac cgcctgagga taccgtcacg
     1561 tcctgcgaac ccggcagttc caacgcgcgc aaacggtagc tgccaatttc gagcggcaag
     1621 ttgagcgatc gccgttcacc tggcggaagc agttgttgga ccacgacgtg cggcgtaaac
     1681 tccggactac caacgcaaaa tgaagttacg gcaacgcgac gaaccgccgg attcggacgg
     1741 aatgtcagtt cgacgtaacg atcgaaatca acggtgaaat cgatcttgca agcttcgcag
     1801 tgaacgtggg cgttgatgtc tcttaatgat tgtcccgact cctgtgggcc acgacacaac
     1861 gggcacaaca agtcccattg aaaatctaat aacccgactc gcgtcgcgcg cagacagatt
     1921 tccagcacgt cgcgtcgcgg ctggttccag tcgtctgcga gtttataagc acgtatgcga
     1981 gtaacggcca ggtcatcggc gtgttcgaga aagtcagcga gacgatcggc gacagcgcgc
     2041 ttctccgccg gcaactgcgc ctgttcgagg tcggcggcaa gtttctggtg caacgacgta
     2101 agccgcgcta gatcaaataa ggactgtgaa gtattaggtt cgctagtctc gatggcgagg
     2161 ccttcagacg cagccgtgtc atatttttga atagcctcac gaaaacggcg cgcgacgatc
     2221 agtttaattt gcagtgatat cgctacggcg cccggaagat ttcgcggcgt ggaccagagc
     2281 tgatacacga ggtgcgtgcc gccgctggta ttcggcgtta actccgccaa cacccgcaaa
     2341 cgcgccagcg gtcctttgtg gtaaatgcgc tcgatgccaa aacgaaacgg cttcacccat
     2401 tcgaacggtt gttcttccca ctcgaccggc acaccataaa gcgaaagccg cactttgcgc
     2461 cgcgcgttgc gcagcggttt atgtgcggcg tcgacctcaa tttccggtac gccagtgtcg
     2521 cgattaaacc ggtttgtgtc agcgatgaag ggccaaagtc tctccggact cgatttgaga
     2581 tcaaactccc atcgtttatg aagctcgcga aaagacattt gccattgccg atttttgatt
     2641 gtcgattgcc gattggggca gctacttgat cgtccatcag aacgcggaaa gtcggtcaaa
     2701 tcggcaatcg aaaatcggca atcgaaaatg attagacttc tttatattca gaatccacat
     2761 cttcatgcga gagtgcgacg cggtagttct tcgactgcag ttgcttggtg atcagcagat
     2821 catctttcgt cttgaggaga tccgcacgac tgtaaacaga aagctcgaag ttgtagccgt
     2881 gcgtaatcgc gtccttagga cacgcctcca cgcagtaacc acaaaaaata caacgtccgt
     2941 aatcgatgtt atagacggcg gcgtaacgtt catcgacgcc gatgcgctct tccggtggac
     3001 gcggatcgtc ggcagcaacg atgtagatac attccgaagg acacgctgcg gcgcacagat
     3061 aacatgccac acacttctcc ctgccgagtt cgtcaacatg caacaggtgc tggccgcgat
     3121 agcgcggctg aacagtaacg ggttcatccg gatactcgac cgtccacttg cgctttggaa
     3181 tgtaagacag cgttacaccc attccctgga tgatcgcttt ggtggattcg ataacgtcgc
     3241 taatgattga catgtactaa gtttaatccg ttctcacagg ttcccaaaga cgcctccaaa
     3301 gaagagcagg aacagcagtc cgtaaaagat gattgtcgcg gctcctgtca cgataccaat
     3361 gatcgccatc ggttttccac cagcagtgtc cggcgtcttt ttgatctgag caagtgccaa
     3421 ccagccaagc acgactgcga ggactcctgt taacggcccg aaacaaccaa acaacaaacc
     3481 gataattcct ataatcatcg aggcggtagc caatccctga ctctgttgct ttatgggtcg
     3541 tggcggtggc ggcggcggtt gccacaccgg ctgcattggt ggacgttcgt ccggcgcgac
     3601 ccaaccgccc ggcgccggcg gatcgcgcgg aagccattgc gtttgtcgct cggaggcagg
     3661 cggtttgact ttcggttcgc ggatggacgg attccagttc ggatccgccg gtggagacaa
     3721 ctcctcgatc agtagcgagc cgtcatccga gcagaaagtg atccacggtt cggtgtactg
     3781 tcgattgcac tgcgaacaat acttcatctg cggtacttca tctgcgcatc agaccaattc
     3841 gagccggctg aagaaatatg cgatctcaat tgcggcgttc tcgtctgagt cggagccgtg
     3901 cacagcgttc tcgcctacgg aaccgccaaa ctcttttcgc agcgtgcctg catcggcctt
     3961 tgcgggatca gtggcgccca tcagatcgcg ccacgcttta acggcgttct ctttttccag
     4021 cgccatcacg acgcatggtc cactcgacat gaactccgtg agtccactga agaacggtcg
     4081 ctcgcgatgt accgcgtaaa atccctcagc ttgcttgcgt gtcatgtgaa tgagttttaa
     4141 accgcggatt ttaaaaccgt cctcgagaat ccgttgtagt attgagcctg tctttccagc
     4201 acgaaccgca tcaggtttga ttattccgaa agtcaatgta gtcatctgtg tcatctgtgg
     4261 ttaaagtgct tccttcatcg ctttgccgat gtcggcgggc gactttacaa cgtgaatacc
     4321 ggcttcatcc aacgccttca tcttttccgc cgcagttccc ttgccgccgg cgatgatcgc
     4381 gcccgcgtgg cccatgcgac ggccgggcgg tgcagtctgg ccggcgataa acgctacgat
     4441 cggtttcgac acgttgtcct ttgcgaacgc agccgcatct tcttcagcgg tgccgccaat
     4501 ctcgccgatc atgacgattc cgatcgtctc cgggtcagct tcgaacagtc ggaacgcgtc
     4561 tacgtgagtt gtgccgacga tcggatcgcc gccgatgccg atcgcggatg actggcccag
     4621 gccgagtgct gtcagttggc ccaccgcttc gtaagtcaag gttcccgagc gcgacacgac
     4681 accgatgttt ccttcacgat gtatatgacc gggcatgatt ccgatcttgc acttgcctgg
     4741 agaaatgatt ccgggacagt ttggaccaat cagacgcgtc tgtctgtcgc tcaggtactc
     4801 tttcaccttc accatgtcga gcgtcggaat accttcagtg atgcaaacaa caactgcgat
     4861 gcctgcgtcg gcggcctcca tgatcgcgtc cgcggcaaac gagggcggca cgtagatcac
     4921 ggacgcgttc gcgccggctt ctctgactgc atcgacgacg gaattgaaaa cgggaattcc
     4981 ctcgtgtgtt gtgccaccct tgccgggcgt cacgccaccg acgacattcg ttccatactc
     5041 aaccatctgc ttggtatgaa acgtcccctc tttcccggta attccctgca cgatcaggcg
     5101 ggtagactta tcaaccagaa cgctcaacga tgaatgctcc ttaaagattt acgggcttta
     5161 gttcaaaagg cggcagtata gtaatccgtg ttatctgtgt ccaacggcct acaccaactc
     5221 aaaccgctcg ccgtactgcg gcacgtggac gttccagccg aagcggtctt tgatgtgtcc
     5281 ggccattgcg ttcgcggctt ccggttcgcc gtgtgttaga aaggtgcgac tgggtacgcc
     5341 ggtctccatg ccttcgagcc attgcaaaac ttccttccag tccgcgtgcg ccgagaagcc
     5401 gccgatcttt gccagacgac aacgaaccgg gatccactgc ttcaggattt taacttccgg
     5461 ttcgccgtcg aggatgcgac ggccggtcgt tcccgccgcc tgaaagccga caaacactat
     5521 cgtcgcctca ggatcgggaa ccatgcgcaa cgcgtgatgc aacacgcgac cgccggtcat
     5581 cattcctgac gcactgatga tcacacgcgc tccctttgca tcgttcactc gcttcgactc
     5641 ctgtcgcgac gttgcattta ccatcgagtg cgttcgcagc ggatggcgtt tctgcatcag
     5701 gatgcttgcg tattcttcgt cgtgttcttc cttgcgattc gcgtatgccg cagtcgcggc
     5761 agcggccatg ggtgaatcga caacgaccgg caatacgggg atcgctttct cgtcttccag
     5821 ttcgcgaatt aaatagacga tctcctgcgt gcgcccaacg gcaaacgccg ggatcaggac
     5881 cggcgcatca cgctgtgcgg cgtcgttgat gatgcgcgcc agcgcgactt tcggatcttc
     5941 gggatcgtga agacgatcgc cgtacgttga ttcgacgagc aggtagtcac atggcggtgg
     6001 tggcgacggg tcgcgaatga tcggctgatc gtagtggccg agatcgcctg aaaagagtat
     6061 gcggcgtgcc ctgccatccg agccggcgcc ttggacctcg gcaagcacca ggctcgatcc
     6121 gagaatgtgt ccggccgttt tgaagctcac gtgaattcca ggcgcaggta cgatcccgtc
     6181 gccctcattg gcgactggcc ggattaagtc aaccgcggca tacgcatcgt cttcggtata
     6241 aagcggcagt gccggatcgt gcaccgtgag tttatggcga ttgcggaagt cagcatcttc
     6301 ttcctgcaac cttccggaat cgggcaaaag gatcttcaaa agatcggcgg tgccgcgcga
     6361 gcagtagact ggaccatgaa atccttgttt cacgagtcgt ggaagatagc cggtatggtc
     6421 gatgtgtgcg tgcgtgatca gcacggcgtt gagcgaacgc ggatcgaacg gcggctcctg
     6481 ccagttgcgt tcacgcagtt ccttcaaccc ctgaaacaga ccgcaatcga cgagcactcg
     6541 cgccttgtcg gtctcgatca aatacttcga tccagtgacc gtccccaccc cgccccaaaa
     6601 tgaaatgctt gccaagatgc tccttacgaa accgttaaaa cggtttctca attcggcttc
     6661 gcacttctca cctggctaaa gccaggtgag aatgaaacaa ggacatttct gtacttgtcg
     6721 gcttcgctaa cctgtgtcat taccggcttc agccgcctgg ctccttctca ttctcacccg
     6781 gcttcaccgg cctgactcct tctcattctc acccggcttc accg
//
LOCUS       DASTEY010000513         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_456229, whole genome shotgun sequence.
ACCESSION   DASTEY010000513 DASTEY010000000
VERSION     DASTEY010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456229"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1735)
                     /locus_tag="VFO72_08990"
     CDS             complement(95..1735)
                     /locus_tag="VFO72_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479468.1"
                     /translation="MCAFCASLWFPPTSHGQTRSQASPQSSISEATALLQAGRFEEAE
                     TVTRQIVTRNPKNAEARGLLGLILDQRGLHAEAEREYQAALRLKPNLVSALSNFGVLL
                     ARTNRPAEAIAKFEEVLRFDSKHERAIFNLGALYAARGDYRRAVPLLEKAAGTPACKP
                     VMPHAGDRALLLTLVNAYVHIDRRQEALGLSRCLEEISANDPRALFTLALSLAEAHEY
                     AEAVRLFQRTNELRPQTYEVLYNLGLALYNLDRLSDAQQALSSASALKPAEADPYYRL
                     GLVASAQGDTKAALTHWTKALEFRPVFPEVNFMIGEELLKNRLAEKSIPFYERALDQS
                     KGQLVYYLRLGVANVRGQRYERAREIFTRALERFPDNANLYFLLGYTARAEGQYDQAV
                     AAFQKVLKLQPDNPDALSNLGYIASQRGEHVEAERLLKRAIQLDPNAYPSYHDLGRLL
                     VKLKRYDEAVPLLKRGVELNDKDPGIRYQLFLAYSRLKRKDEADKELAEFKRLDEVNR
                     HAATPLGQTVTTGSAMAGETLPPLPARASGDSAKPRTP"
     gene            complement(1796..>2137)
                     /locus_tag="VFO72_08995"
     CDS             complement(1796..>2137)
                     /locus_tag="VFO72_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479469.1"
                     /translation="QESQGLELNRAAFRCAQTPIFPNEPNGPHIGTLGRNVFRGRPQF
                     YWDASLFKNIPIPAISEAFNVQFRFSAYNVLNRVNRSAPNGDINNANDFARDINEQRR
                     RQMEFSLKLIF"
BASE COUNT          467 a          595 c          609 g          466 t
ORIGIN      
        1 gatagaagga gcggattgtg caagaacagt ggtagagaca aaggcaatta tgattgagac
       61 gagggcagcg cggcgcaaag gttcgactcc aatcctaagg tgttcggggt ttcgcactgt
      121 cgcccgaagc tcgcgccggt aagggcggta gcgtttcacc ggccattgct gaaccagtcg
      181 tcaccgtctg gcccagaggc gtcgcagcgt gccggttcac ttcgtcaagc cgcttaaact
      241 cggccagttc tttgtcggcc tcatcctttc gtttcaatcg cgagtaagcc aggaagagtt
      301 gataacgtat gcctggatcc ttgtcgttca gttccacgcc gcgcttcagc aacggcactg
      361 cctcgtcgta acgtttgagt ttcaccagca aacgccccag atcgtgataa gaaggatacg
      421 cgttcggatc gagttgaatt gcgcgcttca acaggcgctc cgcttcgacg tgctcaccgc
      481 gctggctcgc gatgtaaccc aggttcgaaa gcgcgtcggg attgtcaggt tgtaacttca
      541 gcactttttg aaaggcggca acagcctgat cgtactgtcc ctccgcccgc gccgtgtatc
      601 ccagcagaaa atagaggttt gcgttatcgg gaaagcgttc gagagcacgc gtgaagatct
      661 cgcgcgcgcg ctcgtagcgc tggccgcgaa cattcgccac tccaagccgc aagtagtaaa
      721 ccagttggcc tttactctgg tccagtgccc gttcgtaaaa cgggatcgac ttctccgcca
      781 atcgattctt taacagctct tcaccaatca tgaaattgac ttcgggaaac accggtcgga
      841 actcgagcgc tttggtccaa tgcgtaagtg cggctttcgt gtcaccctgc gcggaggcaa
      901 ccagcccgag acggtaatac ggatcggctt cagccggctt aagtgctgaa gcggatgaca
      961 gcgcctgctg tgcatcagac aatcgatcga ggttgtagag cgcgagacca agattgtata
     1021 ggacttcgta cgtctgcggc cgcaactcat tcgtgcgttg aaacaaacgc acggcctcgg
     1081 cgtattcatg tgcttccgcc agactcaaag ccagcgtgaa aagtgcgcgc ggatcgttcg
     1141 cgctgatctc ttcgagacag cgcgaaaggc cgagcgcttc ttgccggcga tcgatatgaa
     1201 cgtaggcgtt aaccagcgtg agcagcaacg cgcgatcgcc cgcgtgtggc atgacaggct
     1261 tgcaagcggg agtgcctgct gccttttcga gcaaaggcac tgcacggcgg tagtctccac
     1321 gcgcagcgta cagcgcgcct aagttgaaga tcgctcgttc atgtttcgaa tcgaaccgca
     1381 agacctcttc gaacttggcg atcgcttcgg ctggacgatt cgtccgtgcc agcagtacgc
     1441 cgaagttcga caaagcagaa accaggttcg gcttcaatcg cagcgccgcc tgatattcgc
     1501 gttcggcttc ggcatgcagg ccgcgctggt cgagaattag cccgagtaac ccgcgggcct
     1561 cggcgttttt tggatttctc gtgacaatct ggcgtgtcac ggtttccgcc tcttcgaaac
     1621 gccctgcctg caacagcgca gtggcttcgc taattgaaga ctggggcgaa gcctgcgagc
     1681 gtgtttgtcc gtgcgatgtg ggggggaacc acagagaggc acaaaaggca cacatcacaa
     1741 accaaatctc tttgtgacgc ttgtgccttt ttgtggctaa cactagaaaa tcacactaga
     1801 agataagctt gagggaaaac tccatttgcc gccgtcgctg ctcgttgatg tcgcgagcaa
     1861 aatcattcgc gttgttaatg tcgccattcg gagccgaacg attcacccga ttcaaaacgt
     1921 tgtaagctga aaagcgaaac tgcacgttga acgcctcgct gatagccgga atcgggatgt
     1981 tcttgaatag cgatgcatcc cagtagaact gcggccggcc gcggaaaacg tttcgtccca
     2041 acgtgccgat gtgcggacca ttcggctcat tcgggaagat cggcgtctga gcgcatctga
     2101 aggccgcacg gttaagttcg agcccctgtg actcctg
//
LOCUS       DASTEY010000514         5224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_818918, whole genome shotgun sequence.
ACCESSION   DASTEY010000514 DASTEY010000000
VERSION     DASTEY010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818918"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..629
                     /locus_tag="VFO72_09000"
     CDS             120..629
                     /locus_tag="VFO72_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479470.1"
                     /translation="MGVTKISIEVVSRIEAGQILCSPGRCAEITYLVSIGDGDDPLPH
                     GFANVERKSRLRIADVVTDEGATVDDVQQIIDLAEQLRSESGTLLIHCEAGVSRSTAT
                     ALIMYAYWLGQGSEDEAMQRVIAQRPYAIPNRRMVALADRLLGLGGNLLRARDESVSS
                     LWADYDGDR"
     gene            complement(667..2628)
                     /locus_tag="VFO72_09005"
     CDS             complement(667..2628)
                     /locus_tag="VFO72_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479471.1"
                     /translation="MSDSTSRLPNRPSLDQLRKQAKELLRQLRSGDPSAAERLRKHKA
                     NVSDPILADSQFVLAREHGFESWPRLVHYIQATKLPGVDQHQRIAEDLVAVYNLAAPE
                     AVTRLNDLFHSTLNLDQIRDFIRDKLFNLPDTERRINNFTLFDAQLVVSNLYGFKDWN
                     ELVQSINEADPDPHSAPYVLSTKPPFYRIDWTNNSIEPRQPMSTGDWENLCAVASELG
                     LTSINSGNLIGDDDLQIISQLDQITSLNLDGSKRLTDKGLQYLARMPQLRELTLGGQI
                     TDRGLQALGHLRELRVFRMFWQANVTDKGIANLSVCDQLEEVDLLGCNTGDGAIAALS
                     GKPKLRRFKTGRNVSDDGLALLHQFPAFKTRPAEEPRFGLMSFSAEPNDLLIDGPFTR
                     QGVNNLRGLDGLFGLSFFWHTSRLRGNDLQGLDGLSNLVYVGCQDQLCDDDAMRHLAA
                     LPKLRMLMGQGAVATDSGFESLSRSQTIEYIWGRECPNLKGPGFVAMSRMPALKGLAV
                     SCKFVDDAALASLPDFPSLKELVPMDVGDEGFRHIGRCEQLESLVLMYCRDTGDVATS
                     YLAGMPNLKKYHAGYTLITDVSLELLSRIKSLEEISFEGCKFITDAGIRFLTTLPHLR
                     EVSIGGCPKVTRSGVTGFARSVRVNYDPR"
     gene            complement(3034..3840)
                     /locus_tag="VFO72_09010"
     CDS             complement(3034..3840)
                     /locus_tag="VFO72_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479472.1"
                     /translation="MKPTILTLAFLLFASAAMAQRTSPPSKAELAQITERGRQLAQYD
                     VAAWYASDAVIATKPAQGTVARYIAKKEGAVWTVMFGRLNEKGDKFLIAYEATQAATP
                     KEFTVKRHNPAKVDTDFFLSAAKAIEISLAEFTGAERPYNVAVLPAASKQLHVYVVPA
                     QTENGVFPLGGDARYLVSADGSKIVEKRQLHKAIIEYKTGENENALQAGFHTAVLDDI
                     PEDTDVFHVLARRPSVPQYVGTKQYVYRIETDGTINYLMRMEEFRKGKGN"
     gene            complement(4144..>5224)
                     /locus_tag="VFO72_09015"
     CDS             complement(4144..>5224)
                     /locus_tag="VFO72_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9479473.1"
                     /translation="RGIGLATDDVKHTWMIYALDKRNGKVLWTNTAFEGVPRAKRHVK
                     ATQANSTPVTDGRYVVALFGSEGLACYDTKGKLLWKQDLGVLNPGLWDDKESSWGHSS
                     SPIIYRDLVIVQADGHKQSFIAAFNLKDGKQAWRVERNEITSWATPTIYQGANRVEII
                     ANGGRFIRGYDPLTGKELWRFADNDTQVKMQAPLVAHDLIYITGGYPPGRAMYAFRPG
                     AVGDISLKSGEEKNEFIVWRTSKGSPYTPTPIIYGDLMYTLADNGVLSAYDAKTGDTI
                     YQQRLPSSFSASPVAADGKLYLASEDGDVFVVKAGRQYELLSRNTMGQPLMATPAVSQ
                     GMLIVRGEKAIYALGERKSAKNWLP"
BASE COUNT         1100 a         1406 c         1459 g         1259 t
ORIGIN      
        1 ttggcaggct tttcagcgtg ccgtagcaac atgatcttgg tggcgcgtcg aagttggttg
       61 ctcatgcgtt gttcagacga ttattaccga taattaagat atcgtgtacc ttgtttttta
      121 tgggcgtgac caaaatcagc attgaagtcg taagccggat cgaagccggc cagatccttt
      181 gttcgccggg acggtgtgcc gaaatcactt acctcgtatc gatcggcgac ggggacgatc
      241 cgcttccgca cggtttcgct aacgtggagc gcaagtcgcg tttacggatc gccgatgtcg
      301 taacagacga aggcgctacg gtggacgacg tgcaacaaat aattgatttg gccgagcagc
      361 ttcgatcgga gagtggcacg ttgctgatcc actgcgaggc cggcgtaagt cgctccacgg
      421 ccaccgcgct gatcatgtac gcatactggt tgggccaggg cagcgaggac gaagcaatgc
      481 aacgcgtcat cgcgcaacgc ccgtatgcga taccgaaccg gcgcatggtg gccctggctg
      541 ataggcttct gggtctcggt ggcaacttgt tacgagcgcg cgacgagtcg gtgtcatcac
      601 tttgggccga ttatgacggc gatcggtaac cccgcactcc caaggcttac gtcacatcga
      661 agcccctcac cgcgggtcgt aattcacgcg cacactgcgc gcaaaccccg tcactcctga
      721 tcgcgttacc ttcggacagc cgccgatcga gacttcgcga agatgcggca gggtcgttaa
      781 aaaccgaatg ccggcgtctg tgatgaattt gcaaccctcg aatgagatct cctcgagcga
      841 cttaatccga ctcaacagct cgagactgac gtcggtaatc aacgtgtaac cagcatgata
      901 cttcttgagg tttggcatcc cggctaaata actcgttgcc acgtcgccag tgtcgcgaca
      961 gtacatcaga accaacgatt caagttgttc gcagcggccg atgtgacgaa acccttcgtc
     1021 gccaacgtcc atcggtacca gctctttcag agaagggaag tcaggcaaac tcgcgagcgc
     1081 tgcgtcatca acgaacttgc aacttacggc caaacctttc aaggccggca tcctcgacat
     1141 cgcaacaaac cccggtcctt tcaagttcgg gcactcgcgt ccccagatgt actcaatcgt
     1201 ttgtgaccga ctcagcgatt caaaaccgct gtcagtagcg acggcgccct ggcccatcag
     1261 catgcggagc tttggcagcg ccgcaagatg tcgcatcgcg tcgtcgtcgc aaagttgatc
     1321 ctgacaaccg acgtaaacga gattcgataa gccatcgaga ccttgcaaat cattaccgcg
     1381 aagacgcgac gtgtgccaga agaaactcaa accaaagagt ccatcgagtc cacgcaggtt
     1441 gttcacgccc tgccgtgtga atggtccatc gattagcaga tcgttgggct cggcactgaa
     1501 cgacatcagc ccgaatcttg gttcttcggc cggtcgcgtc ttgaacgcgg gaaattggtg
     1561 cagcagggcg agaccgtcgt cgctgacgtt gcggccggtc ttgaagcgac gcagtttggg
     1621 ttttcctgac agtgcggcga tcgcgccgtc gcctgtgttg cagcccaata gatcaacttc
     1681 ctccaattga tcgcacacgc tgagattcgc aattccttta tcagtgacgt tggcttgcca
     1741 aaacatgcgg aagacacgca attcgcgcag gtgaccaagt gcttgcaggc cacggtcggt
     1801 gatctgaccg ccaagcgtca attctcgcaa ttgcggcata cgggcgaggt actgaaggcc
     1861 tttgtctgtc aggcgttttg agccgtcgag gttgagcgac gtgatctgat cgagttgcga
     1921 aatgatttgg agatcgtcat cgccgatgag atttcccgag ttgatgctcg tgagtcccag
     1981 ttcgctcgcg acggcacaga ggttctccca atctccggtc gacattggct ggcgtggttc
     2041 gatgctgttg ttggtccaat cgatgcgata gaaaggaggc ttggtgctca agacgtatgg
     2101 cgccgaatgt ggatcgggat cggcttcgtt aatcgattgc actaactcgt tccagtcttt
     2161 gaagccgtac aggttggaga cgacaagttg cgcatcgaat aacgtgaagt tgtttatgcg
     2221 tcgttcagtg tcaggcaggt tgaacagctt gtctcggatg aagtcgcgaa tttggtcgag
     2281 gttgagcgtg ctatgaaaga gatcgttcag tcgcgtcacg gcttccgggg cggccaggtt
     2341 gtagaccgcg acgagatctt ctgcgatccg ctggtgctgg tccacgcccg gcaatttagt
     2401 ggcctggatg tagtgaacga gcctgggcca gctttcgaag ccgtgctcgc gagcaaggac
     2461 gaactgggaa tctgccagaa tcggatcgga aacgtttgct ttatgtttgc gcagccgttc
     2521 ggcggcagac ggatcaccac tgcgtaattg gcgcagcaac tctttggctt gtttgcgaag
     2581 ttggtccagt gaaggacggt tgggtagacg agatgtggaa tcagacatgt tgatctcctt
     2641 tcgttaccgc ccgtgtccgc acgcgggcca aaagaagatc gagtttgtag aagatagatc
     2701 gcaggtgggc tcagcccttt ccgcggacgg gttgcggcct gttacgcggc agtaatagta
     2761 accggccggt tatcggtgag caacggtgat gagacaacag caatgaagtg tttttcaaca
     2821 taactttcct ttcgttaccc actttgacaa taaaggggct aaagcgggtg aagaggtctc
     2881 gcgcaaaggc gcaaagacgc aaagtgtact cgtgtttcta actgctttct tttgcgtctt
     2941 tgcgccttgg cgcgagaaat attctgccca cagagcacgc acatagattt gagtactttt
     3001 tgtgcaaagc cttcgttacc cacaaagcga aaatcaatta cctttccctt tcctgaattc
     3061 ttccatcctc atcagataat tgatcgtgcc gtcagtttcg attcgataga catactgttt
     3121 tgtgccgaca tactgcggca cggacggccg gcgggccagg acgtggaaga cgtctgtatc
     3181 ttcgggtata tcgtccagga cagcggtgtg aaagcctgct tgtaacgcgt tctcattctc
     3241 acctgttttg tattcgatga ttgctttgtg cagttggcgt ttttcgacga tctttgaacc
     3301 gtcggccgaa acaaggtagc gtgcgtcgcc gcccaatggg aaaacgccgt tctccgtttg
     3361 tgcgggaacg acgtacacgt gcaactgttt cgacgcggca ggcagaactg cgacgttata
     3421 aggtctttct gcgcctgtga attcagctaa tgaaatctcg atcgctttcg cggcagagag
     3481 aaagaagtct gtgtctactt tggcaggatt gtgcctcttc acagtgaatt cttttggcgt
     3541 cgcagcctgg gtggcttcgt aggcaattaa aaacttgtcg cctttctcat ttaaacggcc
     3601 aaacatgacg gtccaaacgg cgccttcttt tttcgcaatg tatctcgcga cggtgccttg
     3661 cgcgggcttt gtggcgatga ccgcatcgct cgcataccag gcggcgacgt cgtactgtgc
     3721 caattgccgg ccgcgctccg tgatttgcgc gagttcagct tttgaaggcg gcgacgtccg
     3781 ctgggccatc gcggcactgg caaacaggag gaaagcgaga gtcaggattg taggtttcat
     3841 cgtttcactt ctttgaagat cgtttggtca cttgagctga ccactggagt ttaggacgta
     3901 taacacatag ggcgttgcct taaaactccc ttactctgct tgttctctgt gcaattctcc
     3961 gtgttccctg tgccttcgtg gtgtttttct aagcaatcgt gaaccaccga gccacagagg
     4021 acaccgaggt agctcagaga agaatgtgtc cacttttatc gaaaagccaa cacatagtta
     4081 gcttttacag cgggcgcgat cccgccgagg taaagacctc gtatcatttg ccaaatccgt
     4141 cgctcagggc aaccagttct ttgcggactt acgctcgccg agtgcgtaga tcgccttctc
     4201 tccgcgcacg attaacatgc cctgcgagac ggccggtgtt gccatcagcg gctggcccat
     4261 cgtgttccgc gacagcaact catactgcct gccggccttg acgacaaaca catcaccatc
     4321 ttcactcgcc aggtacagct ttccgtccgc cgcaaccggt gaggcactga acgagctcgg
     4381 cagtcgctgt tgatagatcg tgtcgccggt ttttgcgtcg taagcgctga gtacgccgtt
     4441 gtccgcaagt gtatacatca gatcgccata aatgattggc gtcggcgtgt aaggactgcc
     4501 cttgctcgtt cgccaaacga tgaattcgtt cttctcttca cctgacttga gcgagatgtc
     4561 gcccacagcg ccgggacgaa acgcgtacat cgcgcgtccc ggtggatagc ccccagtgat
     4621 gtagatcaga tcgtgcgcga ccagcggcgc ctgcattttt acttgcgtgt cgttatcggc
     4681 gaagcgccag agttccttgc cggtcaacgg atcgtagccg cgaatgaacc gcccgccgtt
     4741 ggcgattatc tccacccggt tcgcgccctg atagatcgtc ggcgtggccc atgacgtgat
     4801 ctcatttcgt tcgacccgcc aggcttgttt gccgtccttc aaattaaacg cggcgatgaa
     4861 cgattgcttg tgtccgtctg cctgtacgat tacgaggtcg cggtagatga tcggactgct
     4921 cgagtggccc catgaagatt ctttgtcgtc ccagagcccg ggattcaaca cgccgagatc
     4981 ttgtttccat agcagcttgc cttttgtgtc gtaacaagca agaccttcgg aaccgaaaag
     5041 cgccacgacg taacggccgt ctgttaccgg agtcgaattc gcttgcgtcg ctttgacgtg
     5101 acgcttggcg cgcggtacac cttcgaaggc ggtgttggtc caaagcacct tgccgttacg
     5161 cttgtcgagc gcgtagatca tccaggtatg cttaacgtcg tcggttgcga gtccgatacc
     5221 gcgg
//
LOCUS       DASTEY010000515         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1593484, whole genome shotgun sequence.
ACCESSION   DASTEY010000515 DASTEY010000000
VERSION     DASTEY010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593484"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /gene="allB"
                     /locus_tag="VFO72_09020"
     CDS             <1..1163
                     /gene="allB"
                     /locus_tag="VFO72_09020"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HET9479474.1"
                     /translation="DTHVHINSPGRTEWEGFRTATRAAAAGGVTTLIDMPLNSIPPTT
                     TLAGFKTKLDTAKDDCFVDVGFWGGVVPGNTPELARMFAAGVVGFKCFLVPSGVDEFP
                     HVTEDDLLRAMPELSRLGAMLIVHAELPGPMKATDGTDYPAFLRSRPRATENHAIELM
                     IRLSREFGTRVHIVHLSSSDALPMLREAQADGVKISAETCPHYLHFTAETIPANATEF
                     KCCPPIRESENREKLWEGLAGGTLEMIVSDHSPCPPGMKARETGDFITAWGGIASLQL
                     RLPVVWTEAHRRGFSLRQLTDWLCTNPARQVSLGSQKGAIATGFDADVVIWDPNREFV
                     VDGGQLEHRHKLTPYHGEPLTGVVEKTFLGGRTIYDCRLGVIGDLPRGHLLL"
     gene            1164..2156
                     /gene="alc"
                     /locus_tag="VFO72_09025"
     CDS             1164..2156
                     /gene="alc"
                     /locus_tag="VFO72_09025"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HET9479475.1"
                     /translation="MEFTELIDLASEKVGGAVLVANDDFFAPKENLLKVSTPIFVEGK
                     YTDRGKWMDGWESRRRRTPGFDWCIIRLGLRGIIRGVVVDTSYFRGNYPEHCSLDGAI
                     FSGLPAEGDLLSESVQWIPLLPQVPLVGDSRNQFSIKHGESITHLRFRIFPDGGVARL
                     RVYGEVVPDWERLRRAGGHIDLAAAENGGLVLSCSDMFFGHRHNLIMPGRADNMSDGW
                     ETKRRRGPGHDWAIIKLGRRGKIRSVEVDTSHFKGNFPESCSLEACNVSGSEDLTDTS
                     VVWTNLLPRTKLQAHTRHYFDRELNDAGAVSHLRFNIFPDGGVSRLRVYGTLAE"
     gene            2161..2682
                     /gene="uraD"
                     /locus_tag="VFO72_09030"
     CDS             2161..2682
                     /gene="uraD"
                     /locus_tag="VFO72_09030"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET9479476.1"
                     /translation="MQTSHGLALLNELTPDEARTELLKCCGAKRWAEAVENQRPYKTV
                     DQLIGRANDVWWSLEQTDWLEAFRSHPKIGENKAAGAVSAQSQHWSAQEQQGVRHAAQ
                     QVIEKLARLNHEYEKKFGFIFIVCATDKSSDEILSLLEQRLPNDAATELRNAASEQAK
                     ITELRLRKLIERS"
     gene            2679..3023
                     /gene="uraH"
                     /locus_tag="VFO72_09035"
     CDS             2679..3023
                     /gene="uraH"
                     /locus_tag="VFO72_09035"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HET9479477.1"
                     /translation="MSTISTHVLDTSRGAPASGVAVSLELQNNDESWSELSHAWTDDD
                     GRVKPFFLVDRPVSNGTYRLVFDTEPYFSSLSIPCFYPQVTVVFNVDDASQHYHVPLL
                     ISPYAYSTYRGS"
     gene            3036..>3332
                     /locus_tag="VFO72_09040"
     CDS             3036..>3332
                     /locus_tag="VFO72_09040"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET9479478.1"
                     /translation="MILDATLSEWLNLLFRWTHVFAGIMWVGTTYYFTWLDARLTEEE
                     KAFATTGTTPQVWMVHSGGFYVVERRKVPDLASRTLHWFRWEAGMTWLSGFALLV"
BASE COUNT          706 a          860 c         1005 g          761 t
ORIGIN      
        1 ttgatacgca cgttcacatc aattccccgg gccgcaccga atgggaagga tttcgaacgg
       61 caacacgggc tgcggctgcc ggcggagtga cgacgctgat cgacatgccg ctcaactcga
      121 ttccaccgac gacaacgctt gcgggtttca aaacaaaact ggacaccgct aaagacgact
      181 gctttgtcga tgttgggttc tggggcggcg tggttcccgg caacacgccg gagctggcac
      241 gtatgtttgc agccggtgtc gttggcttca aatgttttct ggtgccttcg ggtgtcgatg
      301 agtttccgca tgtaacagag gatgatctgc tgcgggcgat gccggagctt tcgcggctcg
      361 gagcgatgct gatcgtgcat gccgaactgc ccggaccgat gaaggccacg gacggcacgg
      421 attacccagc atttctccgg tcgaggccgc gtgcgacgga gaatcatgcg atcgaattga
      481 tgattcgttt gagtcgtgaa ttcgggacgc gcgtgcacat cgttcatctc tcttcgtctg
      541 acgcgttgcc aatgctgcgc gaagcgcaag cggacggtgt gaaaattagt gccgagacat
      601 gtccgcacta tcttcacttc acagccgaaa caattccggc taacgcgacg gagttcaagt
      661 gttgtccgcc gatacgcgag agcgagaatc gggagaagct ctgggaaggt ctggctggcg
      721 gaacgctcga gatgatcgtt tcggaccatt cgccgtgtcc tccgggtatg aaggcgcgtg
      781 agactggcga cttcatcact gcgtggggag gcattgcgtc gctgcaactg cgtctgccgg
      841 tggtttggac cgaggcccat cggcgtggat tttcgttgcg tcagctaact gactggcttt
      901 gcactaatcc ggctcggcag gtttcgctcg gttcacaaaa aggcgcgatc gcgacgggtt
      961 ttgatgcgga tgttgtcatc tgggatccga atcgagagtt cgttgttgac ggcggacaat
     1021 tggaacatcg ccacaaactg acgccttatc acggcgaacc gctgacgggc gtcgtcgaaa
     1081 aaacttttct tggcggaaga acgatatatg attgtcgcct tggcgtgatt ggtgatcttc
     1141 cgcggggtca tctgcttttg taaatggaat tcacggagtt aatagatctt gcttcggaga
     1201 aggttggcgg tgcggtgctt gtggccaacg atgatttctt tgcgccgaaa gagaacctgc
     1261 tgaaggtgag cacgccgatt ttcgtcgaag ggaagtacac ggaccgcggc aagtggatgg
     1321 acggatggga aagccggcgc cggcgcacgc ccggattcga ttggtgcatc atccgtttag
     1381 ggttgcgggg catcattcgc ggcgtcgtgg tcgacaccag ttatttccgt ggtaattatc
     1441 cggaacactg ctcgctcgat ggtgcgatat tttcaggact accggccgaa ggggatctcc
     1501 tgagcgagtc agttcagtgg attccgctgt tgccgcaggt gccgctcgtc ggggattcgc
     1561 gtaaccagtt ttccatcaaa catggtgaaa gcatcactca tctccgtttc cgcatctttc
     1621 ccgatggtgg cgttgcacgg ctgcgcgtct atggcgaagt agtgcctgac tgggaacgac
     1681 taagacgcgc cggtggccac atcgatttgg cagcggccga gaatggcgga cttgtgctgt
     1741 cgtgtagcga tatgtttttt ggtcaccgcc acaatctaat catgcccggc cgggcagata
     1801 atatgagcga tggctgggag acgaaaaggc gtcgtgggcc aggacatgat tgggcgatta
     1861 tcaaactcgg ccgtcgggga aagattcgtt ctgtcgaagt tgacacgtcg cacttcaagg
     1921 gcaactttcc ggagagctgt tcgctcgagg cgtgcaacgt ttcgggctcg gaagatctta
     1981 cggatacatc cgtcgtgtgg accaacctgt taccacgcac gaaacttcag gcgcacacgc
     2041 gacattactt cgatcgggaa ctgaacgacg ccggagccgt gtcgcatctg cggttcaata
     2101 tttttcccga cggcggagtc agtcgtttgc gtgtctatgg cacgctggcc gagtaaaaac
     2161 atgcaaacgt cgcacggtct ggcgttgtta aatgaactca ctccggacga agcgcgtaca
     2221 gagttgttga aatgttgtgg cgctaagcgc tgggccgaag cagtcgaaaa tcagcggcct
     2281 tacaagactg tcgaccaact gatcggccgc gcaaacgacg tttggtggtc gctcgaacag
     2341 acggattggt tggaagcgtt tcgtagtcac cccaaaatcg gcgaaaataa agcggccggc
     2401 gcagtttcgg cgcagtctca gcattggtcg gcgcaggagc aacaaggcgt gcggcacgcc
     2461 gcgcagcaag taatcgaaaa actggcgcga ctgaaccacg aatacgaaaa gaaattcggt
     2521 tttattttta tagtttgcgc cactgacaag tcatctgatg agatactgtc gctgctcgaa
     2581 cagcgtttgc caaacgatgc ggcgacagag ttgcggaacg cagcttcaga acaagcgaag
     2641 atcacagagt tgagattgcg aaaactaatc gaacgctcat gagcacgatt tcaacccacg
     2701 tactcgacac ctcgcgcggc gcgcccgcga gcggcgtggc tgtctcgctc gagctgcaaa
     2761 acaatgacga gagctggagc gagctgagcc acgcatggac cgacgacgac gggcgcgtga
     2821 agccgttctt tctcgtcgat cgcccggtat cgaatggaac ttaccggttg gtttttgata
     2881 ctgagccgta tttctcatcg ctcagtatcc cctgcttcta ccctcaggtg acggtggttt
     2941 tcaacgtcga tgatgcttct cagcattatc atgtgccgtt gcttatcagt ccttacgctt
     3001 attcgactta tcgcggaagt taataactcg ccacgatgat cctcgacgcg accctgagcg
     3061 aatggctcaa tctactgttt cgttggacac acgtctttgc cggcatcatg tgggtcggca
     3121 cgacttacta cttcacgtgg ctcgatgcac gcctgactga ggaagaaaaa gcattcgcta
     3181 ccacaggcac gacgccgcag gtctggatgg tccatagcgg tggcttctac gttgtcgaga
     3241 gacgtaaagt gcctgacctc gcgtcgcgga ccttgcactg gtttcgttgg gaagccggca
     3301 tgacgtggct cagcggcttc gcgttgctgg tg
//
LOCUS       DASTEY010000516         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_11520, whole genome shotgun sequence.
ACCESSION   DASTEY010000516 DASTEY010000000
VERSION     DASTEY010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11520"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2796
                     /locus_tag="VFO72_09045"
     CDS             <1..2796
                     /locus_tag="VFO72_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9479479.1"
                     /translation="GNYTIAGLAPGKYTLRVVNAGFAMYENAEVEVVAGKAEQLDVTL
                     KVAIEEQRVTVAADNRELSTEPENNANAVVLKGEDLDSLPDDPDDMAAALQALAGPSA
                     GPNGGQIFVDGFTGGRLPPRASIREIRINSNPFSAEYDRLGFGRIEILTRPGSDRFRG
                     QASLNFNDDALNARNPFADRRPPIQTRQYGGNFGGPLIKGKSSFFVDFDKRDLDDETL
                     IVATVLDPNNNIVGFTETAPVPSRRTSFSPRVDFQISPNHTLIARYNYTRNARVVGVG
                     GFSLPSRAYDTANTQQSIQLTETSVINKTIVNETRFEFEHSRNEQDADNSIPTIEVSE
                     AFTGGGSQVGQSHSTDKEIELTNNTSFVKGQHSVKTGARFRWISVSQFSPQNFGGTFT
                     FFGGAASLTSIERYRRTIVLLEQGFTPAEIRAMGGGASQFRLSSGNPQTDVSQWDVGG
                     FLQDDWKVRPNLTLSLGLRSENQKNIDSNLNFGPRLGFAWSPGGQQSKTVVRGGYGVF
                     YQRVGENLTMQALRLNGVNQQQFTVLNPDFFPLIPTVDQLISFAVPGTVYRLADGLQA
                     PYTLQGVFSVERQLPRNLTIAASYINIRTLHVLRTRPLNAPLPGTFTPGVPNSGIRPL
                     DCADFIPPEINPAPRCNIFEYESSGRYNQNQFIVNFNSRLHRNASMNAYYVLAKANSD
                     ADGTGSFPANPYDLSTEYGRASGDIRHRFVMTGNFRAPWGISLNPFIIVQSGRPFNIT
                     LGRDLNGDTLNTERPALAPAGADCSDTVNFRCTPCGNFKLTLAPGDVMIPRNFGQGPG
                     STSVNLRVSKTWSFGSEGGDANANQQNPRGQGNPGQRAIIGGMGRGPGGGGGRGGFGG
                     GPRGGFGGGGGGRYNLTFSVNFQNLLNHPNFGNPVGNLGSALFGQSTFTTGGFGGFGG
                     GSAAYNRRIDAQIRFSF"
     gene            complement(2797..3339)
                     /locus_tag="VFO72_09050"
     CDS             complement(2797..3339)
                     /locus_tag="VFO72_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479480.1"
                     /translation="MKIPRVISLFIYAAILATASSAFAQESNAAREKTRERLTALLQR
                     VGPDLNVSFKPTSKSPFVFTGMMRDGLKNSDQFEIVISVTTKDTISFRIFPHYKGAYI
                     NIDKAKNSAQLMRKLVQLNDSTFLFWGADDSGDVFTGYTFTLESGFPDKAIEIVLSSI
                     KNSDQFVGELRPLIDGSSAQ"
     gene            3493..4737
                     /gene="thrC"
                     /locus_tag="VFO72_09055"
     CDS             3493..4737
                     /gene="thrC"
                     /locus_tag="VFO72_09055"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9479481.1"
                     /translation="MCSRCGDLLDVERTERHVQDVETFRALWLERLKSNEARDRSGVW
                     RFREFLPFASDVPVVSLGEGNTPLYDAPRSADYCGLAALKLKHQGNNPTGSFKDTGMT
                     VAVTQALKLGMRLVACASTGNTAASLAAYAARARMLCVILVPEGQVSDAKLAQALDYG
                     AKVLEIEGNFDVCMRVIQELAEQSSLYLVNSINPFRIEGQKTVAFELAEQLEWNVPDH
                     LVVPGGNLGNSSALGKGFRELLGHGLTRKQPRISVIQAEGAAPFARFAENSDSEFVNE
                     EHPRTLASAIKIGAPVSWRKAWRAVRETGGQVITVSEQEIADAKAMIGRDGIGCEPAS
                     ATTVAGIRKLVSDCLINKDENVVAVLTGHILKDTDYVIKYHSQTLTAPETEKHIRGTF
                     ANSITRVAARPCAIESVLKGLS"
     gene            4701..>5191
                     /locus_tag="VFO72_09060"
     CDS             4701..>5191
                     /locus_tag="VFO72_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479482.1"
                     /translation="MCDRIRFEGFIVMTEIRVPASTSNLGPGFDCFGLALKVYLTVRA
                     TAAPEASEPCRVTTTGAKENEVLPRNAANLIYRALAFAARREGVSLPPVELVAHNEIP
                     LASGLGSSAAAIVAGIKLLSLLTGHQMTDQAVLDYATEFEGHPDNVAASLCGGFVINC
                     LQK"
BASE COUNT         1110 a         1481 c         1483 g         1117 t
ORIGIN      
        1 ggcaattaca ccatcgccgg gctagctccc ggcaaataca cgttgcgcgt agtgaacgcc
       61 ggctttgcga tgtatgaaaa cgcggaagtc gaagtggttg ccggcaaggc tgaacaactc
      121 gacgttactt tgaaagtcgc gatcgaagag cagcgcgtga ccgtcgccgc cgacaatcgc
      181 gagctgagca ctgaacccga aaacaacgcg aacgcggtcg tgctgaaagg cgaggacctc
      241 gactcactgc ctgacgatcc ggatgacatg gcggcggcgt tgcaagcact cgccggtccc
      301 tccgctggtc caaacggcgg ccagatcttc gtcgacggtt tcaccggtgg ccgcttgccg
      361 ccgcgtgctt cgattcgcga gatccgtatc aactccaatc cgttctcagc ggaatacgat
      421 cgactcggtt ttggccgcat cgagattctg acgcgacccg gcagcgaccg ttttcgcgga
      481 caggcctcgc tcaacttcaa cgacgacgcc ctgaatgcgc gcaacccttt tgccgatcgt
      541 cgtcccccaa ttcaaacacg ccagtatggc ggaaacttcg gtggcccact gatcaagggc
      601 aagtcatcgt tcttcgtcga cttcgataaa cgcgacctcg acgacgagac gctgatcgtc
      661 gccacggtac tcgatccaaa caacaacatc gttggtttca ccgagacggc gccggttccg
      721 agtcgccgga cgtcgttcag cccgcgcgtc gactttcaga tcagtcccaa ccacacgcta
      781 atcgcgcgtt ataactacac gcgcaacgct cgcgtcgtcg gcgtgggtgg cttttcgctt
      841 ccctcgcgcg cctacgacac ggccaacacc caacagagca ttcagctcac tgaaacctca
      901 gtcatcaaca aaacgatcgt caacgaaacg cgcttcgagt ttgagcacag ccgcaacgag
      961 caggatgccg acaattcgat tccgacgatc gaagtgtccg aagcgttcac cggtggcggt
     1021 tctcaggtcg gccagtcgca cagcactgac aaagaaatcg aactgacgaa caacacgtcg
     1081 ttcgtcaagg gccagcactc ggtgaagacg ggcgcccggt tccgctggat cagtgtttct
     1141 cagttctcgc cgcaaaactt cggcggcacg ttcacgttct tcggtggtgc tgccagtctc
     1201 accagtatcg aacgctatcg acgcacgatc gttttgctgg agcagggctt tactccggca
     1261 gagattcgcg cgatgggcgg cggagcgagc cagtttcgct tgtcgtccgg aaatccgcag
     1321 acagacgtca gccagtggga cgttggcggt ttcttacagg acgattggaa agttcggcca
     1381 aacctgacgc ttagccttgg cctgcgttca gagaaccaga agaacatcga cagcaatctt
     1441 aactttggcc cgcgactcgg ttttgcatgg tcgcccggcg gccagcaatc caagactgtc
     1501 gtgcgcggcg gctacggcgt cttttaccag cgtgtcggtg aaaatctgac gatgcaagcg
     1561 ctgcgtttga acggcgtcaa ccagcagcag ttcaccgtgt tgaatcctga cttcttcccg
     1621 ctgattccta cagtggacca gttaatctcg tttgcggttc ccggaaccgt ttaccgtctc
     1681 gccgacggcc ttcaagcgcc ttatacgttg cagggggtgt tcagcgttga acgtcagttg
     1741 ccacgcaatc tcacgattgc tgccagctac atcaacatcc gcacgctgca cgtgttgcgc
     1801 actcgcccgc tgaacgcgcc gctgcccggc acgtttaccc ccggagttcc aaacagcggc
     1861 attcgcccgt tggactgcgc ggatttcatt ccgcccgaga tcaatccggc gcctcgctgt
     1921 aacatatttg agtacgagtc gagtggccgc tacaaccaaa accagttcat cgtcaatttc
     1981 aacagccgcc ttcatcgcaa cgcctcgatg aacgcgtatt atgttctggc aaaggccaac
     2041 agcgacgctg acggaacggg aagtttcccg gcgaatccct acgacctgag caccgagtac
     2101 ggccgcgcgt cgggcgacat ccgtcatcgc ttcgtgatga ctggtaactt ccgcgcgccg
     2161 tggggcatct cgttgaatcc attcatcatt gtgcagtcgg gccggccttt caacatcacg
     2221 ctcggtcgcg acctcaatgg cgacacgctg aacaccgaac ggccggcgct cgcgcccgcg
     2281 ggagctgact gttccgacac tgtaaatttc cgttgcacgc catgtggtaa tttcaaacta
     2341 acgttggcgc ccggcgatgt gatgatcccg cgcaacttcg gtcagggtcc cgggtcaacg
     2401 agtgtgaact tgcgcgtcag caagacgtgg agttttggtt cggaaggcgg cgacgccaat
     2461 gccaaccaac agaatcctcg gggccaggga aacccaggtc agcgagcgat catcggcggc
     2521 atgggccgag gacctggtgg cggcggcggt cgcggcggtt ttggtggtgg tccgcgcggt
     2581 ggcttcggcg gcggtggtgg cggacgctac aacctgacgt tctcggtgaa tttccagaac
     2641 ctgcttaacc acccgaactt tggtaatccc gttggtaacc tcggttcggc gttgtttgga
     2701 cagtccactt ttacgaccgg cggctttggc gggtttggtg gcggcagcgc agcttacaac
     2761 cgaagaatcg acgcgcagat caggttcagc ttttaattac tgagcgctac tcccgtcgat
     2821 caatggccgc agttcgccga caaactgatc cgagttcttg atgctcgaca ggacgatctc
     2881 tatcgcttta tccggaaagc cggactcgag cgtgaacgtg tagcctgtaa aaacgtcacc
     2941 cgaatcatca gcgccccaaa acagaaacgt tgaatcgttt agctgtacca gcttgcgcat
     3001 cagttgcgcg ctgttttttg ctttgtcgat gttgatgtaa gcgcctttgt agtgcggaaa
     3061 gatccggaag ctaatcgtgt ccttcgtcgt aacgctgatc acaatctcaa actgatctga
     3121 attcttcaat ccgtctcgca tcatgcccgt gaaaacaaac ggcgactttg aagtcggctt
     3181 gaagctaacg ttcaagtcgg gcccgacgcg ttgcagcagc gcggtgaggc gttctcgcgt
     3241 cttttcgcgc gcggcattcg actcctgagc aaaggctgaa gaagcagtcg ctagaattgc
     3301 ggcataaata aataaactaa tcacacgggg gatcttcatc cggttctcct ttgtcaaaac
     3361 aaacctgccc tcacctttag gtgaagtgtg aggcaatgtc aatagttttg atccgttgta
     3421 tgcttaggct gcgtgtttgt gcagcgctgc atagattgtg gaagcgagtt tgatctcaac
     3481 gagcgcgtct acgtgtgttc gcgatgtggc gacttgctcg acgttgaaag aacagaacgt
     3541 cacgttcagg acgtggaaac gttccgtgcg ttgtggcttg aacggttgaa gtctaacgaa
     3601 gctcgcgatc gaagcggagt ctggaggttt cgcgagttct tgcctttcgc ttcggacgtg
     3661 ccggttgttt cgctcggcga aggcaacacg ccgctttacg acgccccccg gtctgcggat
     3721 tactgcggac ttgctgccct caaactgaaa catcagggta acaatcccac cggctcgttc
     3781 aaagacaccg ggatgacggt ggctgtaacg caggcactga agttagggat gcgcctcgtc
     3841 gcatgcgcca gcactggaaa cactgctgca agtcttgcgg cttatgcggc gcgtgcgcga
     3901 atgctttgcg tgatcctcgt gcctgaaggt caggtaagcg atgcgaagtt ggcgcaggcg
     3961 cttgactacg gcgcgaaggt gctggagatc gagggaaatt tcgacgtttg catgcgcgtc
     4021 attcaggaac tggccgaaca gagttcgctc tatcttgtta actcgatcaa tccgtttcgc
     4081 attgagggac aaaagaccgt agcgttcgag cttgcggaac aactcgaatg gaacgttccg
     4141 gatcatctcg ttgtgcctgg cggaaatctg gggaatagtt cggcgttggg gaagggattt
     4201 cgtgaattgt tgggccacgg attaacacgg aaacagccac ggattagtgt gattcaggca
     4261 gagggcgcgg cgccgtttgc gcggtttgcg gagaatagcg actcagagtt cgtaaacgaa
     4321 gaacatccac ggacgttggc gtcggcgata aagataggcg cgcctgtgtc gtggaggaaa
     4381 gcgtggcgcg ctgttcgcga aaccggcggt caagtgatca ctgtcagcga acaggagatc
     4441 gccgatgcga aagcaatgat cggtcgcgat gggatcggtt gcgaaccggc gtcggcgaca
     4501 acagtcgcgg gcattcgcaa gctggttagc gattgtttga tcaacaagga cgagaatgtc
     4561 gtcgcagtgc ttaccggaca catactcaaa gacactgact acgtgatcaa ataccacagc
     4621 caaacgttga ccgctcccga aaccgaaaaa cacattcgcg gtacgttcgc gaactccatt
     4681 acaagagtgg ccgcgagacc gtgtgcgatc gaatccgttt tgaagggttt atcgtgatga
     4741 cggagattcg tgttccagct tccacttcga accttggccc gggcttcgac tgcttcggcc
     4801 tcgcactcaa agtttatctg actgtgcgcg ccactgccgc gcccgaagcg tccgagccgt
     4861 gtcgcgtcac taccaccggc gcgaaagaga acgaagtgtt gccacgcaat gctgccaacc
     4921 tgatctatcg cgcgttggct tttgcagcgc gtcgcgaagg cgtttcgttg cctccagtcg
     4981 aactcgtcgc ccacaacgag atcccgcttg cgagcggtct cggaagtagc gccgctgcaa
     5041 tcgtcgcggg catcaagttg ttgtcgctac tcaccggcca ccaaatgacc gaccaggcag
     5101 tgcttgacta cgcgaccgag ttcgaaggtc atccggacaa cgtcgccgcc tcgttatgcg
     5161 gcggtttcgt gatcaattgc ctgcaaaaag a
//
LOCUS       DASTEY010000517         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_555219, whole genome shotgun sequence.
ACCESSION   DASTEY010000517 DASTEY010000000
VERSION     DASTEY010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555219"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFO72_09065"
     CDS             <1..756
                     /locus_tag="VFO72_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479483.1"
                     /translation="RAKAVQFFRDGLSFLSKDDCEKALPYFEKAVESDNNYAEAWAQA
                     GFCNEKLGKHAEALEASKKAVSLRPSAESYFNIGLASFYLKQYKDAAEAYRQAIKIDP
                     YNAADAYYALGLVYRDWAKPDDEIQAYKQAIRLKPDYTVAYERLGSRYLRSKKFNEAV
                     EIFRQLSALKPGDAIAPNNMGEAYFELNRLNDALESFRQSVRLKPDYGKAYYNLGRTL
                     LAMGNRDAALEQYTILTNIDPDWAEKLNSIINP"
     gene            complement(778..2166)
                     /locus_tag="VFO72_09070"
     CDS             complement(778..2166)
                     /locus_tag="VFO72_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479484.1"
                     /translation="MSSACISARRTPNLDHIFASARARSGKRPVIVIPGVLGSELINP
                     KTGETVWPSAFRTSQEGLPMSPDLAANRDDLVPGKIIETVKLARVLPEVYVYRDLLNA
                     LRRYAGYRQGDWENPGAGDYQDTVYVFPYDWRQDNVRNARELVRRIERLKSRLQRPDL
                     KFNVVAHSMGGLIARYAAMYGDADLPEGDGPIQPTWAGAAHISKIVMIGVPNEGSTDA
                     FATLIEGYSITEGLRRRVPLLNKLTAQDVVRTPSVFQLMPHRGAVRFLDENLQPLALD
                     LYDVDVWKRYGWSVIYSSPDFRRNYIGAAGTDGGNGKSEELDAYLAATLRRALRFHQA
                     LDSVENSNSPVVLLAIGGDCEETLSAPVILRDEKRNRWLTLTRPREFRTSSGAKISKK
                     QATNAMFAPGDGRVTRTSLLGETLFKHRDTQTGFTLSHYAVFGCDLHGQLPRNKSVQD
                     NALTAIVGEVMN"
BASE COUNT          522 a          657 c          642 g          481 t
ORIGIN      
        1 cgcgcgaaag cggtgcagtt ctttcgcgac ggtttgagtt ttctttccaa ggacgattgc
       61 gagaaggcgc tgccttactt cgaaaaggct gtcgagtcag acaacaacta cgccgaagca
      121 tgggcacagg ccgggttctg caacgagaag cttggcaaac acgcggaggc gctggaagct
      181 tcgaagaaag ccgtcagttt gagaccctcc gctgagtcgt acttcaacat cgggctcgcg
      241 agtttctacc tcaagcaata caaggatgcg gctgaagcgt atcgccaggc gattaagatc
      301 gatccgtaca acgctgccga cgcttattac gcgctcgggt tggtctatcg cgactgggcc
      361 aagccggacg atgagattca ggcgtacaag caggccattc gactaaagcc cgattacacc
      421 gtcgcctacg aacgactcgg ctcgcgctac ctgcgctcga agaaattcaa cgaggccgtg
      481 gagatctttc gccagctttc cgcattgaag ccgggagacg ccatcgcgcc gaacaacatg
      541 ggtgaggcct atttcgagtt gaaccgcttg aacgatgcac tcgagtcgtt ccgtcaatcc
      601 gtccgtctaa agccggacta cggcaaagcc tactacaacc tcggccgaac cctgctcgca
      661 atgggcaacc gcgacgccgc cctcgagcag tacaccatcc tcaccaacat cgaccccgac
      721 tgggccgaga agctgaactc tataattaat ccgtaaacaa tccgcgtaaa cccgtggcta
      781 attcatcact tcgccgacaa tggcggtgag ggcgttgtct tgcacagact tgtttcgagg
      841 aagttggccg tgtagatcgc agccgaagac tgcgtagtga gagagcgtga agcctgtctg
      901 ggtgtcgcgg tgtttgaaga gggtctcgcc gaggagcgat gtgcgtgtga cgcgtccatc
      961 gcccggtgcg aacatggcgt tagtggcttg ctttttcgag atcttcgcgc cgctcgaggt
     1021 gcggaattcg cgtggtctgg ttagcgtcaa ccaacgattc cgtttctcat ctcgcagaat
     1081 caccggcgcg ctcagagtct cttcacagtc accgccgatt gccaggagca ctaccggaga
     1141 atttgagttc tcgacggagt cgagcgcctg gtgaaatcga agcgcacgtc tcaaagtcgc
     1201 ggcaagataa gcatcaagct cttccgactt gccgttaccg ccgtcggttc ccgcggctcc
     1261 gatgtaattg cgacgaaagt cgggagacga atagatcacg ctccatccat atcgcttcca
     1321 aacgtcaacg tcatacagat cgagcgctaa cggttgcagg ttctcatcga gaaaccttac
     1381 cgccccgcga tgcggcatga gctgaaacac cgaaggagtg cgcaccacgt cttgggcagt
     1441 cagcttgtta agaagcggaa ctcttcgtcg tagcccttcg gtgatcgagt aaccttcgat
     1501 gagggttgca aacgcatctg tcgaaccttc attcggaact ccgatcatca cgatcttgct
     1561 gatatgagcg gcgccggccc aggttggttg aattgggccg tcgccttcag gcaagtccgc
     1621 gtcgccatac atcgcggcgt agcgtgcaat caacccgccc atcgaatgcg ccacgacatt
     1681 gaatttgaga tcgggacgtt gcagtctgct cttcagtcgc tcgatgcgtc tgacaagctc
     1741 tcgtgcgttt ctgacgttgt cctgccgcca gtcgtaagga aagacgtaga ccgtgtcctg
     1801 gtagtcgccg gcaccgggat tctcccaatc accttgacga tatcctgcgt aacggcgcaa
     1861 cgcattaagc agatcacggt agacgtacac ctcgggcagc acgcgcgcaa gctttactgt
     1921 ctcgattatt tttccgggaa cgagatcgtc gcgattggcg gcgaggtccg gactcatcgg
     1981 cagtccttcc tgcgaagtgc gaaacgcgga tggccaaacg gtttcacccg tcttcggatt
     2041 gatcagttca gaaccaagaa ctcccggaat cacgatcacg ggacgttttc ccgaccttgc
     2101 ccgcgccgaa gcaaagatgt gatcgagatt cggcgtgcgg cgcgcggaaa tacatgcgct
     2161 cgacaccagc gaaagtatga taaggatgaa gagtatttgc cgggagcgag caactgcgaa
     2221 tctattcaac gggcaccgtc caacgacgat tgatgaggct ggcgacaaac ccggcaccaa
     2281 agccgttgtc gatattcact ac
//
LOCUS       DASTEY010000518         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_225801, whole genome shotgun sequence.
ACCESSION   DASTEY010000518 DASTEY010000000
VERSION     DASTEY010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225801"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1420)
                     /locus_tag="VFO72_09075"
     CDS             complement(89..1420)
                     /locus_tag="VFO72_09075"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9479485.1"
                     /translation="MATKIDTMTGEEMVELSRRHTLFEWSVQSAVDPIPVARAKGIYF
                     WTPEGKRFIDFNSQLMCVNIGHGDERVVRAIQKQAETLSYANPFMATEVRARLAAKLA
                     EITPGDIDTFFFTNGGAEANENAIRIARLFTGRHKIGARYRSYHGGTAGVLTLTGDPR
                     RWGAEPGIPGVFRIPDFYHGIQKGGYDTTEEVLARTEEIVELEGPKTIAAIIVEPVVG
                     TNGILIPPDGYMQGLRALCDKHGILLIADEVMAGFGRTGEWFSVDHWKLVPDLMTMAK
                     GLTSAYVQLGAVGMRRQIAEHFQKNFFYGGLTYNSHPLACAAAIATISVYEEDKLIEN
                     ARRMGQLMRSLLEDLQTRHPSVGATRNIGLFGIVELVRNRETREPLAPFNSTSPEMQA
                     LGKFFRQEGLYTFVRWNTFFTNPPLCINEAELREAFAIIDRGLEITDQAVT"
     gene            1499..2593
                     /locus_tag="VFO72_09080"
     CDS             1499..2593
                     /locus_tag="VFO72_09080"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9479486.1"
                     /translation="MSKTAAFMGMASLRNLVTDSIAIDLGSAATIIYVRGSGVVVDEP
                     SLVAVNNISGEVIAVGLEARQMYGREARDVTVVAPLLNGVVADFERTREMLAHFVRQA
                     RSGSSFFSRRALMSVYPGVTHVERRALLTAAEHARIGRVMMMDEGLAAAFGAGVKLHD
                     VRASAVVDIGSGTTNVAVVGNGAIIHSRSERIGSADINSAIINHIRRHRGLAIGAPSA
                     ERLKIELASATLPDDLAREITVRGRDVVTGSPGAITITAGEVYPVANYVVAKIADCVS
                     MTLAELSPEVAGDIYDRGIVLAGGGALFDGIAEYMSEYTKLNVKIADEPRYAIVRGLA
                     QMFDEPLLLRRVVRSDVSPLLDEENEAYSL"
     gene            2668..>3243
                     /locus_tag="VFO72_09085"
     CDS             2668..>3243
                     /locus_tag="VFO72_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479487.1"
                     /translation="MKRFRSLLASVLCAVLLCSLVTSSLAQTTRKQVKIRGYITQLNS
                     PTSFEIEDYRVKRDESLTLELENQNPEVNFKQSDLRIGTLVEITGEYDEQTSELKATK
                     IKVDLEQFRQLDVTTILDRKPLELAQTDSGWRGLIAADGRRIRIEPETKLVFKLNKSE
                     QKNAQDKQKTEKQKSDKQNADQQTTAATEEVV"
BASE COUNT          698 a          933 c          919 g          693 t
ORIGIN      
        1 gcctgcggtc ccagggaaga gcgcgctcag gtaaacagtg attgctgaaa ctgcttcact
       61 gcgaatgccc ggcaaagtaa agtcggactt aggtcactgc ctggtccgta atctccaacc
      121 ctcgatcgat aatcgcaaac gcttcgcgca gctccgcttc attgatgcac aacggcgggt
      181 ttgtaaagaa cgtgttccag cgcacgaacg tgtagagtcc ttcctgacgg aagaacttcc
      241 ctagcgcctg catctccggt gaagtgctgt tgaagggcgc aagcggctcg cgagtttcgc
      301 gatttcgtac cagctcaaca atcccgaaaa gaccgatgtt acgggtcgcg ccgacggacg
      361 gatggcgcgt ctgaagatct tcaagcaggc tgcgcatcag ctgtcccatc cggcgcgcgt
      421 tttcgatcag tttgtcttcc tcgtaaacgc tgatcgtcgc gatggccgcg gcgcaggcga
      481 gtggatggct gttgtaagtc aaaccaccgt agaagaagtt cttctggaag tgttcggcaa
      541 tttggcgtcg catgcccact gcgcctaact gcacgtacgc actcgtcagc cccttcgcca
      601 tcgtcatcaa atcgggtacg agcttccagt ggtctactga gaaccactcg ccggtgcgac
      661 caaagcctgc cattacttcg tcagcgatta gaagaatccc gtgcttgtcg cagagcgcgc
      721 gtaatccctg catgtaacca tcgggcggaa tgaggatccc gtttgttcca accactggtt
      781 cgacgatgat cgcggcgatc gtttttggtc cttcgagttc gacgatctct tccgtgcgtg
      841 ccagcacttc ctcagtcgtg tcgtaaccac ccttctgaat tccgtgatag aagtcgggga
      901 tgcgaaacac accgggaata cctggctcgg cgccccagcg ccgtggatct ccggtcaacg
      961 ttaaaacgcc ggccgtaccg ccgtgataag accgataacg tgcgccgatc ttgtgccgcc
     1021 cggtaaacaa gcgcgcgatt cggatcgcgt tttcgtttgc ttcggcgccg ccgttggtga
     1081 agaagaaagt gtcgatgtcg cctggtgtaa tctcagcgag tttcgctgcc agtcgtgcgc
     1141 gcacttcggt cgccatgaag gggttggcgt atgacaatgt ctcggcttgc ttttggatcg
     1201 cacgaactac gcgttcgtcg ccgtgaccga tgttcacgca cattaactgg ctgttgaagt
     1261 cgatgaaccg cttcccttcc ggcgtccaga agtaaatgcc tttggcgcgc gcgacgggaa
     1321 tcggatctac tgccgactgc acggaccatt cgaagagtgt gtgccggcgc gagagttcga
     1381 ccatctcctc gccggtcatg gtatcgattt ttgttgccat ggcccgcgat gctactcaaa
     1441 tcaggaaaga ttgacaacgt cataaccttt acacattatc cgtgttgtca tgggattcat
     1501 gagcaagaca gcggcgttca tgggcatggc gtcgctccga aacctcgtta ccgattcgat
     1561 cgccatcgac ttgggatcgg cagccacgat catctacgtg cgtggcagcg gcgtggtcgt
     1621 cgatgaaccg tcgctggtcg ccgtcaacaa catctcgggc gaagttatcg ccgttggatt
     1681 ggaagcgcga cagatgtacg ggcgcgaagc acgcgacgtc acggtggttg cgccgttgct
     1741 caacggcgtc gtcgcggatt tcgaaagaac gcgggaaatg ttggcccact ttgtgcggca
     1801 ggcgcgcagt ggctcgtcct ttttcagccg ccgcgcgctg atgagtgtct atcctggcgt
     1861 gacacacgtg gaaagacgcg cgttgctgac tgcggcagaa cacgcgcgca tcggccgcgt
     1921 gatgatgatg gacgaaggcc tcgccgccgc tttcggcgcc ggtgttaagc tccacgacgt
     1981 gcgtgcttca gcggtggtgg atatcggcag cggcactaca aacgtcgccg ttgttggtaa
     2041 cggagcaatt attcactcgc gttcggaacg catcggcagc gctgacatca actctgcgat
     2101 cattaaccac atacgccgcc atcgcggcct tgcgattggt gctccatccg cagaacgcct
     2161 caagatagaa ctcgcctccg cgactttgcc ggacgacctt gcgcgtgaga tcaccgtcag
     2221 aggacgcgac gttgttacag ggagtccggg cgctatcact atcaccgctg gtgaagtcta
     2281 tccggtggcg aactatgtag ttgcgaagat tgcagactgc gtctcgatga cactggctga
     2341 gttgtcgccg gaagtggccg gcgacatcta cgatcgcggc attgttctag ctggcggcgg
     2401 cgcactcttc gacggcatcg ccgagtatat gagtgaatac actaaactga acgtgaagat
     2461 tgccgacgag ccgcgctacg cgatcgtccg cggcttggcc cagatgttcg acgaaccgct
     2521 cctgctccgg cgtgtggtcc gcagcgacgt ctcccccttg ctggacgagg agaatgaggc
     2581 gtattcttta tagcgttcgg cggccagact gccgttagat cttccgctcc aagccttgca
     2641 aaacccaacc tctggaggcg ttcccagatg aagagatttc gatcgctcct ggcgtccgtt
     2701 ctttgcgctg ttttactgtg ctcgctcgta acctccagcc tggcccagac gacacgtaaa
     2761 caggtaaaga ttcgcggcta tatcacgcag ctcaattcgc cgacttcgtt tgagattgaa
     2821 gattatcgcg tcaagcgtga tgaatccctc acgcttgaac tcgagaatca aaatccggaa
     2881 gtgaacttca agcaatcgga tcttcgcatc ggaacgctcg tcgagatcac cggtgagtac
     2941 gacgagcaaa ccagcgaact gaaagcgacc aaaatcaagg tcgacctcga gcagtttcgc
     3001 caactcgacg tcacgacgat tctcgatcgc aaaccgctcg agctcgcgca aaccgattct
     3061 ggatggcgcg gcctcatcgc cgcagacggc cgccgtatcc ggatcgagcc ggaaacgaaa
     3121 ctagtattca aactcaacaa gtcagaacag aagaacgccc aggacaaaca gaaaaccgag
     3181 aaacagaaat cagacaagca gaacgcagat cagcagacga cggccgccac cgaggaagtg
     3241 gta
//
LOCUS       DASTEY010000519         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1247757, whole genome shotgun sequence.
ACCESSION   DASTEY010000519 DASTEY010000000
VERSION     DASTEY010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247757"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2239
                     /locus_tag="VFO72_09090"
     CDS             <1..2239
                     /locus_tag="VFO72_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479488.1"
                     /translation="ETLRAIRHGEVDALVVDGPQGEVIYTLSTADYPYRLMIDEMNQG
                     AVSVSPDAVILYSNRNFATILGLNGKNVSGTQFGDFVAPQMRDQFLQDLQKSRSEGVR
                     REYLLKHKSGREVPVLISFAELQPQTNSVGIVVTDLTAQKELEEELRKAKEGLEEQVA
                     ERTKELRESESRLFRILEHSAADLKAMRRLNEIGTRCARDGHDRDGCLQEVLKVAIEI
                     SGADKGNIQLVDPTSGRLNMATQSGFASPFLEFFSDNNQAGACNVALETQQRVIVEDV
                     RQSALFVGSPSLSVMNQAGVRAVQSTPLVSSAGNVLGVISTHFSTTRRPTEQELRLMD
                     LLARQAADYLERKRVEEEREELLEREHELRQTAEEANRLKDEFLAIMSHELRNPLNVI
                     LGYAELLLRSDEVRGSASLRRMADAVKRNAVAQSKLIRDLLDLSRLRSGKLELNRELV
                     SPVVSIHNAIETVRTEAAEKNITIEVDAPEEAIFVKADPVRLEQVIWNLLNNSVKFTP
                     NDGRIAVRLRVADGEIVLTVEDNGLGIDASFLPHIFEIFRQADSSANRSQSGMGIGLA
                     VVQQLVELHGGSVTADSEGIGQGASFTIRLPRSVERKSEASPPPDLSLDSLEGMNVLV
                     VDDSEDTTQMVRHLLEISGATVTAVTSGFEALRVAREKHFDVVLSDISMPGMDGFEFL
                     NRLRGLPGYRDLPAIALTGFGRPEDVERAHAQGFYAHLTKPFDIEALASLLQKMPRQS
                     AQQAL"
     gene            2255..>3075
                     /locus_tag="VFO72_09095"
     CDS             2255..>3075
                     /locus_tag="VFO72_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9479489.1"
                     /translation="MKIKLVPFCLLLFVISALAQAPDRQQFIRIEAPLIALTNVRVID
                     GTGAAPREDQTILISGGKIQSVSPTASTTYPADARTLDLKGYTVLPGLVGMHNHMFFP
                     MGGSPPMYSNMGSSFPRLYLALGVTTIRTTGSVAPYTDLELKRLIDAGRVLGPKMHVT
                     APYLEGRGAFTPVMHQLKDADDARRMVNYWADLGATSFKAYMNITREQLRAVVEEAHK
                     RGLKVTGHLCSIGFREAAEIGIDNLEHGLLANSEYVTDKKADACPQGAGMMSLINL"
BASE COUNT          677 a          849 c          915 g          634 t
ORIGIN      
        1 agagacgctg cgcgctattc gacacggcga agtcgacgca ctggtcgtcg atggtccgca
       61 gggcgaagtg atctacacac tctcgacggc ggattatccc taccgtttga tgatcgacga
      121 gatgaaccag ggcgcggtaa gcgtttcgcc ggatgcggtc attctctaca gcaatcgaaa
      181 cttcgccacc atactcggtc tcaacggaaa aaacgtcagc ggcacgcaat tcggcgattt
      241 cgtggcgccg caaatgcgcg atcagttttt gcaggatttg cagaaatctc gcagcgaagg
      301 tgttcgtcgt gaatacctgc tcaagcacaa atctggaagg gaagtaccgg tcctgatctc
      361 tttcgccgaa ctacagccgc aaacaaactc agtcggcatc gtagtgaccg acctcaccgc
      421 gcagaaagag ctggaagaag aactgcgcaa ggccaaggaa ggtcttgagg aacaggtggc
      481 ggagcgcacc aaggagttac gcgaaagcga gagccgcctg tttcgcattc tcgaacatag
      541 cgctgccgat ctgaaagcaa tgcggcggct aaatgagatc ggtactcggt gtgctcgtga
      601 cggtcacgat cgcgacgggt gtttgcagga ggttctgaag gttgcgatag aaatcagcgg
      661 cgcagacaaa ggcaacattc aattggtaga tcccacctcc gggcggctca acatggcgac
      721 gcagtctggt ttcgcttcgc cgtttctcga gttcttttca gataacaatc aggctggggc
      781 ctgcaatgtt gcactagaga cgcagcaacg cgtgatcgtg gaagacgtgc ggcagagtgc
      841 gctgtttgtt ggcagtccat cattgagtgt aatgaaccag gcgggtgttc gtgcggtgca
      901 gtcgactccg ctcgtgagca gcgcgggcaa tgtcctcggc gtgatctcta cgcacttcag
      961 caccactcgc agaccgacgg agcaggagtt gcgcctgatg gacctgttgg cccggcaggc
     1021 agcggactat ctcgagcgca aacgcgtgga agaggaacga gaggagctat tggagcgcga
     1081 gcacgaatta cgccagacag cggaagaagc gaaccgcttg aaagacgagt ttctggcgat
     1141 catgtcgcac gaactgcgca acccgctcaa cgtgatcctc ggttacgctg agttgttgtt
     1201 gcgcagcgac gaggttcgcg gttcagcgtc tttacgccgg atggccgatg ccgtcaagcg
     1261 caacgcggtg gcccaatcaa aactgattcg cgacctgctg gatctttcgc ggttgcgcag
     1321 cggcaaactc gagttgaatc gcgaactcgt gtcgcccgta gtttcgattc acaatgcgat
     1381 tgaaacagtg cgtaccgaag cggcagagaa gaatatcacg atcgaagtgg acgcgccgga
     1441 agaggctatc tttgtcaaag ccgatccggt caggctggaa caggtaatat ggaacctgtt
     1501 gaataactcc gtgaagttca cgcccaacga cgggcgcatc gccgtcagac ttcgcgttgc
     1561 ggacggtgaa atcgttctga cggttgaaga caacgggctg ggaatcgacg cttcgttcct
     1621 gccgcacatc tttgagatct ttcgccaggc ggactccagt gcaaatcgct cgcaatccgg
     1681 gatgggaatc ggactcgccg tggtccagca actggtcgaa ttgcacggcg gctcggtcac
     1741 tgctgattcc gaaggcatcg gccagggcgc aagtttcaca attcgcctgc cccggagcgt
     1801 cgagcgtaaa tcggaggcct cgccgccgcc cgatctttct ctcgactcgc tcgagggaat
     1861 gaatgtgctc gttgttgacg actccgaaga taccacgcag atggtgcgcc acttgttgga
     1921 gatcagcggc gctaccgtga ctgccgtcac tagtggcttt gaagcgctgc gtgtcgcacg
     1981 tgagaaacat tttgatgttg tattgtcgga tatctcgatg ccgggcatgg acggctttga
     2041 gtttctgaac agactgcgtg gtttgccggg ttatcgcgat ctgccggcaa ttgcgctaac
     2101 gggttttggt cgtcccgaag acgtggagcg tgctcatgct cagggcttct acgcgcacct
     2161 gacaaagcct ttcgacatcg aggcactggc aagcctctta caaaaaatgc cgcgacagag
     2221 cgcacagcaa gcgctttgac gtaaaatccc cggcatgaaa atcaaacttg tccccttctg
     2281 cctgctgctg ttcgtcattt ccgcgctggc ccaggcgccg gatcgccaac aattcattcg
     2341 catcgaagca ccgctgatcg cactgaccaa cgtgcgtgtg attgacggca cgggcgccgc
     2401 gccgcgcgag gaccagacga tcctgatctc cggtgggaaa attcaatccg tcagtccgac
     2461 tgcaagcacg acttatcccg cggacgcgcg cacgctggac ctcaagggtt ataccgtcct
     2521 gcccgggctc gtcggcatgc acaaccacat gttctttccc atgggtggct cgccgccgat
     2581 gtactcaaac atgggcagca gttttccgcg tctgtatctc gcgcttggcg tgacgacgat
     2641 tcgtactacg ggcagcgttg cgccttacac ggatctcgag ttgaaacgtt tgatcgatgc
     2701 gggacgcgtg cttggtccaa agatgcacgt gaccgcgccg tatctcgaag gccgcggcgc
     2761 gttcacgccc gtgatgcacc agttgaaaga cgccgacgac gcgcgccgca tggtgaacta
     2821 ctgggccgac ctcggcgcta cgtcgttcaa ggcttacatg aacatcacgc gcgaacagtt
     2881 gcgcgcagtc gtggaagagg ctcacaagcg tggtttgaaa gtaacgggcc atctctgttc
     2941 gatcggtttt cgtgaggcgg cggagattgg catcgacaat ctcgaacacg gattgcttgc
     3001 aaactcggaa tacgttaccg acaaaaaggc cgacgcatgt ccccaaggtg cgggcatgat
     3061 gagcctgatc aacct
//
LOCUS       DASTEY010000520         7642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_571928, whole genome shotgun sequence.
ACCESSION   DASTEY010000520 DASTEY010000000
VERSION     DASTEY010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571928"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VFO72_09100"
     CDS             complement(<1..1249)
                     /locus_tag="VFO72_09100"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HET9479490.1"
                     /translation="MNNYILSLITWLPAVGAILILALMKKDQSNLVKRFATIWFVLDF
                     ILSLWLLKYDRVTGGMQFLEDYQWIPVIGARYQMGVDGVAVLLIILTTLLGAIAALSS
                     WEYIQKREKEYYVLLLLLQTAVIGVFSSMDLFLFYLFFEVSLVPMYFLIGIWGGENRL
                     YAAIKFFLYTLVGSVVMLLGVLKMYFLTQDTTLANQITQESVRLLAGNDAAAQLVSSS
                     LQAAAANGTGTFNILYMQAMGSVLPMGTLQILLFVAFALGFAIKVPMFPFHTWLPDAH
                     VEAPTAGSVILAGILLKLGTYGFYRFNLPMFPAASRDQTTWVTSFGTFGVRSVMVFLA
                     IVSIIYGALAAMYFVVKRDGDVKKLVAYSSVSSMGMVMLGLFSLNPNGVNGGVMQMVN
                     HGIYSGALFLLVGINYERRHTRNG"
     gene            complement(1260..3692)
                     /gene="nuoL"
                     /locus_tag="VFO72_09105"
     CDS             complement(1260..3692)
                     /gene="nuoL"
                     /locus_tag="VFO72_09105"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET9479491.1"
                     /translation="MYKYIWLVPLLPLLGAAINGLFGRKFRFSETVIGGIAVGSVALS
                     FLISVAAVYSYGHAQWPNPYLTSQDGFSFNWIAGGAVNSDQGFALLNIEWSYQLDPLS
                     AVFMLIVTGVGLCIFVFATGYMHGETGLYRFFAYMGLFMFAMLVLVMGSNFVMMFVGW
                     EGVGLCSYLLIGYYFDRQEAANASRKAFITNRIGDFGFALAIFGVIAVFGSAQYTQVF
                     ELARAYPVETLGQWGVLSWIALGLFIGACGKSAQLPLHVWLPDAMAGPTPVSALIHAA
                     TMVTAGLYMVTRTNVIFQHSQTMLLVVALVGAATAIFAATIGITQNDIKKVLAYSTVS
                     QLGFMFMACAVGAFVIGIFHVMTHAFFKALMFLGAGSVIHGMHHEQDMRRMGGLKKYM
                     PYTYWTFLAGWLAICGIIPFSGFWSKDEILWNAASTTQIPLGWLVWLVGTIAAIFTAF
                     YMTRLVAMTFWGRERFLEAPAGGEADEAHASAYDKGLTPHDATYPAAGDRPRHEPGEH
                     VGPEPEDVVSHVAPGHAGHGHGVHMPHESPPSMWVPLVVLAVLATVGGLVGIGPAFNF
                     ITGTDHPGGRLNIVNWLNPIIWDPATGDFKTVEGGHGEAAAEPAGEHGSEPLAEGTHE
                     AATGFNLAHAVESATHSHLVTEWIFIGISLLAAGIGMLLGALFYLWRPQLPVIWAQRL
                     RPLYLASYNKYWVDEFYGWAVTRRTMDAARAVFAFDSKLVDGIVNGLATLSRGFSRVV
                     GGIDKYFVDGLVNTIAAFIVRLMSPLLRAAQTGFTQNYALVMVLGLMIAVAAFFARDI
                     AAMLRGFFAAIM"
     gene            complement(3694..4110)
                     /gene="nuoK"
                     /locus_tag="VFO72_09110"
     CDS             complement(3694..4110)
                     /gene="nuoK"
                     /locus_tag="VFO72_09110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET9479492.1"
                     /translation="MNMLNSFIPALAFLFQEVVEGGRFAQMRHAATHPDLSKFLVIGA
                     LLFIIGVAGVLTRRNIIVIFMSIELILNAANLNFVAFSRYLQETGSVNAVAGQIFAVF
                     VIVVAAAEAAIGLGIVIALYRNKETIWVDEIDLLKW"
     gene            complement(4107..4790)
                     /locus_tag="VFO72_09115"
     CDS             complement(4107..4790)
                     /locus_tag="VFO72_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET9479493.1"
                     /translation="MTLSGYEALFFWIFALAAIFAGLLTVMARNAVHCALFLISSLVS
                     VAALFVLAGAEFIAGVQILVYVGGVMVLFLFVIMLVNVGAEEKGREQIFNKQRQVTAS
                     LIFCFLLVAGVLYAVNEGYRGMQKLNPLPEATAGGPTSAEQAGPPNATGTSGMTDDTE
                     RVGNSLYRYASLPFEIASVLLLVAIIGSVMLARTLKQEASADDVDPEVLQEEIARDPE
                     LAKLEPHSE"
     gene            complement(4836..5198)
                     /locus_tag="VFO72_09120"
     CDS             complement(4836..5198)
                     /locus_tag="VFO72_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET9479494.1"
                     /translation="MDAEELKQRTKLFALRILKLVEALPNNTQGRAVGGQLIRAGTSV
                     ASNYRAACKGRSKAEFIAKLGTVEEEADESAFWLELIIEGALLKAKLVEPLLNEANEL
                     TRIMASSRITARSRSRKP"
     gene            complement(5270..5803)
                     /locus_tag="VFO72_09125"
     CDS             complement(5270..5803)
                     /locus_tag="VFO72_09125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET9479495.1"
                     /translation="MPLVKTPKPTWAQKLKRFFMIDLIKGLGLTLKYNLGAITDKDAV
                     AGKGIYTEQYPKVRPDVAPRFHGAPRLNMDPETHDTLCIACNLCALACPEDCIDVIAM
                     DVEIIVAGKPRRKKVLDEFVFDTSRCMFCALCQEACPTSCLELTQDFELATYSRAGFV
                     WNREMLEQGRETVKYTK"
     gene            complement(5957..7144)
                     /gene="nuoH"
                     /locus_tag="VFO72_09130"
     CDS             complement(5957..7144)
                     /gene="nuoH"
                     /locus_tag="VFO72_09130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET9479496.1"
                     /translation="MTLVALWVAYATYLERKISAFIQARLGPMRVGPWGLLQPIADGL
                     KLLTKEDFIPENADRWIFFFAPYIAVASAFIVFSVVPFGPDWAVIADVNIGLLLVLAV
                     SSVGVLALILAGWSSNSKYALLGGLRSSAQMVSYEVAMGLSLIGALMFARTLSLSGIV
                     AAQGSDSIWFILYQPAGFLLFLISGIAENNRAPFDLPEAESELVAGFHTEYSGMRWSL
                     FFMAEYAAMVVVAAVAVTVFLGGWYFPFVYRLEQSGYHNLYVIVSLLVFLVKVSIILY
                     IYFWLRWTLPRFRYDQLMDIGWKWLIPSALLNIALSALAIFLVQALDGRRGMKTVEFI
                     DRGLNLTMQGKIIMIVIGVAGLFITALLMSRINWRSRDFNLKSQRRNIRLVNLPKGKP
                     AVS"
     gene            complement(7260..>7642)
                     /locus_tag="VFO72_09135"
     CDS             complement(7260..>7642)
                     /locus_tag="VFO72_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HET9479497.1"
                     /translation="DMDFDIPLGENGDTYDRYLVRLEEMRQSRRIVVQALDNLPDGPV
                     NAKLPKVVKPPAGDYYHSIEGPKGEIGCYLVSDGSAQPYRVRVRPPSFVNLQALKQMC
                     VGHLVADVVAIIGTLDIVLGEIDR"
BASE COUNT         1926 a         2211 c         2016 g         1489 t
ORIGIN      
        1 cgccgttacg cgtgtggcgc cgctcataat tgatgccgac gagcaggaac agcgcgcccg
       61 agtagatgcc gtgattgacc atctgcatca cgccgccatt gacgccgttc gggtttaatg
      121 aaaaaaggcc gagcatcacc atacccatcg acgatactga cgagtaagcg acaagcttct
      181 ttacgtcgcc gtctctttta accacgaaat acatcgccgc caacgcaccg tagatgatgc
      241 tgacgatcgc gagaaagacc atcacactgc gaacaccgaa agtgccaaag cttgtgaccc
      301 atgtggtttg atctctcgag gctgcgggaa acatcggcag gttgaagcgg tagaagccgt
      361 aagttccgag tttcagcagg atgccggcaa gaatcacaga gccggcggtt ggcgcctcga
      421 cgtgcgcgtc aggcagccag gtgtgaaacg gaaacatcgg cactttgatg gcaaagccga
      481 gggcgaaagc gacgaacaag agaatctgta gcgtacccat tggcaacaca gatcccatcg
      541 cctgcatgta tagaatattg aaagtgccgg tgccgtttgc cgcagccgct tgcaacgagc
      601 tcgacaccaa ttgagccgct gcgtcgttgc cggcgagcag acggacgctc tcctgcgtga
      661 tctggttcgc taaagttgtg tcctgcgtga ggaagtacat cttcaacaca cccagcaaca
      721 tcactaccga accgacaagc gtataaagaa agaacttgat cgctgcatag agacggttct
      781 cgccacccca gatgccaatc aggaagtaca tcggtacgag cgacacttca aagaacaggt
      841 agaacagaaa gaggtccatc gaagaaaata cgccaataac ggccgtctgc aacaacagca
      901 acaaaacgta atactctttc tcgcgcttct gaatgtattc ccacgacgac aaggcggcga
      961 tcgcgcccag cagcgtcgtc aagatgatca gcaacacggc gacaccatcc acgcccatct
     1021 ggtagcgcgc gccgatgact ggtatccact ggtagtcctc cagaaactgc atgccgcccg
     1081 tgacgcggtc gtatttcaac aaccacaacg acaggatgaa gtcgagcaca aaccagatcg
     1141 tcgcaaatcg cttgaccagg ttcgactggt ctttcttcat gagcgccagg atgaggatgg
     1201 cgccaaccgc cggcagccag gtgattaaag aaagaatgta gttgttcata tgtttgtaat
     1261 cacatgatcg ccgcgaagaa acctcgcagc attgcggcga tgtctctcgc gaaaaacgcg
     1321 gccacggcga tcatcaatcc aaggaccatc acgagtgcgt aattctgagt gaaaccagtt
     1381 tgcgctgccc ggagcaaagg cgacatcaaa cgcacaatga atgcggcgat cgtgttgacg
     1441 agaccatcga cgaaatactt gtcaatgcca ccgacaacgc ggctgaaacc tcggctcaac
     1501 gtcgcgagtc cgttgacgat gccgtcgacc agtttcgaat caaacgcaaa aacagcacgc
     1561 gcagcgtcca tcgtgcggcg agtgacggcc cagccgtaaa actcgtcaac ccagtatttg
     1621 ttgtagctcg cgagatagag cggtcgcagc cgttgcgccc agatgacggg caactgagga
     1681 cgccagagat agaagagagc tccaagcaac atgccgatac cagcagcgag cagtgaaatg
     1741 ccgatgaaga tccactcggt taccagatga ctgtgagtag cgctttccac ggcgtgggcc
     1801 aggttaaatc cagtggcggc ttcatgcgtg ccttccgcta gcggctccga tccgtgttca
     1861 cctgccggtt cagcagccgc ttctccgtga ccaccttcaa ccgtcttaaa atctccggta
     1921 gccggatccc agatgatcgg attaagccag ttgacgatgt tcagcctgcc gccaggatgg
     1981 tccgttccag tgatgaaatt gaaagctgga ccaatgccca ccagaccgcc gacagttgcc
     2041 agcaccgcca aaacaaccaa cggcacccac atcgacggcg gcgactcgtg cggcatgtgc
     2101 acgccatgtc cgtgcccggc atgtccgggc gccacgtgcg acacgacgtc ttccggctct
     2161 ggtccaacgt gctcgccggg ttcgtgacgc ggacggtctc ccgccgccgg ataagtggcg
     2221 tcatgcggcg tgagaccttt gtcataagcc gaggcatgtg cttcgtctgc ttcaccaccc
     2281 gccggcgcct cgagaaatct ttccctgccc cagaacgtca tcgccaccag gcgcgtcatg
     2341 taaaaggcgg taaagatcgc cgcgatcgtt ccaacgagcc agacgagcca gcccagcgga
     2401 atttgtgtcg tcgaagctgc gttccagaga atttcgtctt tggaccagaa gccgctgaac
     2461 gggatgatgc cgcagattgc aagccaaccc gctaagaaag tccagtacgt atagggcatg
     2521 tacttcttca aaccgcccat gcgacgcatg tcctgctcgt gatgcatgcc gtgaatcacg
     2581 ctgcccgcgc cgagaaacat gagcgccttg aagaacgcgt gcgtcatcac atggaagatg
     2641 ccaatcacaa acgcgcccac agcgcaggcc atgaacatga aaccgagttg ggataccgtc
     2701 gagtaagcca ggactttctt aatgtcgttc tgagtaatgc cgatagtggc ggcgaaaatc
     2761 gcggtcgcgg cgccgacaag cgctacgacg agcagcatgg tttgcgagtg ctgaaagatt
     2821 acgttggtac gcgtcaccat gtagagtccc gcggtgacca tagtggcggc gtggatcagt
     2881 gcggagactg gagttggacc agccatcgca tccggcagcc aaacgtgcaa cgggagttgc
     2941 gcggacttgc cgcaagcgcc gatgaagagt cccaacgcga tccacgacag cacgccccac
     3001 tgcccgagcg tttccaccgg gtatgctctt gcgagttcga aaacttgtgt gtactgagcg
     3061 ctgccgaaga ctgcgatgac gccgaagatt gccagggcga agccgaagtc gccgatgcga
     3121 ttggtaatga atgctttgcg tgaggcgttg gcggcttcct gccgatcaaa atagtagccg
     3181 atgagtaagt aggaacagag acccacgcct tcccagccga caaacatcat cacgaagttg
     3241 gaacccatca ccagcacgag catcgcaaac atgaacagtc ccatgtaagc gaagaagcga
     3301 tacaacccgg tctcgccgtg catgtatccg gtggcgaaga cgaagatgca caacccgact
     3361 ccggtgacga tcagcatgaa gaccgccgaa aggggatcga gttggtagct ccattcgatg
     3421 ttgagtaatg cgaaaccttg atcggagttg accgcaccac ccgcgatcca gttgaaactg
     3481 aaaccgtcct ggctggttaa gtaaggattg ggccactgcg catggccgta cgagtacacg
     3541 gctgccacac tgatgaggaa agacaatgca acggacccaa ctgcgatgcc tccgatcact
     3601 gtctccgaaa acctaaactt acgaccgaag agaccgttga tggcggcgcc gagcagtggt
     3661 agcaggggaa ctaaccagat gtatttgtac atttcaccac ttcagcagat cgatttcgtc
     3721 tacccagatg gtttctttat tgcgatagag cgcgatgacg atgcctaaac cgatcgccgc
     3781 ttcagcagcg gccacgacga tcacaaagac ggcgaagatc tgccccgcga ccgcgttcac
     3841 gctgccggtt tcctgtaagt aacgagagaa ggcgacaaag ttgagattgg cggcgttgag
     3901 aatcaactcg atcgacatga agatgacgat gatgttgcgc cgcgtcagca ctcccgccac
     3961 gccgatgata aaaagcagcg cgccgatcac cagaaatttc gacaggtcgg gatgcgtggc
     4021 cgcgtgcctc atctgcgcga aacggccgcc ttcaacaact tcctgaaata aaaatgcaag
     4081 cgcgggaatg aaagagttga gcatgttcat tcagagtgtg gctccaactt cgcgagttcc
     4141 ggatctcgcg cgatctcttc ttgcaagacc tcgggatcga cgtcgtcggc ggaagcttcc
     4201 tgtttcagcg ttcgcgccaa catcacggag ccgatgattg ccaccagcaa caagacactt
     4261 gcgatctcaa acggcaacga tgcgtaacga tagagactgt tgcccacgcg ttctgtgtcg
     4321 tctgtcatac cactcgtgcc ggtggcattt ggtggtccgg cttgttccgc gctcgttggc
     4381 cccccggcag ttgcttccgg caacggattg agtttctgca tgccgcggta accctcgtta
     4441 accgcgtaga gcacgccggc caccagcagg aaacagaaga tcaaactcgc cgtgacttgc
     4501 cgctgcttgt tgaagatctg ttcgcggcct ttctcctccg cgccaacgtt gacgagcatg
     4561 atcacaaata gaaacaggac catgacgccg ccgacgtaga ccagtatctg cacgcctgcg
     4621 ataaactccg ccccggcgag cacaaacaag gccgccacgg aaacgagcga cgagatcaga
     4681 aacagcgcgc agtgcacggc attgcgcgcc atcaccgtca gcagccccgc aaagatcgcg
     4741 gccagcgcga agatccagaa gaagagagct tcgtaaccgg agagagtcat tgtcttattg
     4801 tcgattgcgg attgccgatt gccgattgcg tttcatcatg gttttcgaga tctcgaccga
     4861 gccgtaatac gtgagctggc cataatccgc gttagctcgt ttgcttcatt aagtaatggt
     4921 tcgactagct tggccttcag aagtgcgcct tcaatgatca actccaacca aaaggcactt
     4981 tcatcggctt cttcctctac ggttcccaat ttggcgatga actccgcttt cgacctccct
     5041 ttgcacgcgg cgcgatagtt cgaggcgact gaagttcctg ctctaattaa ctgtccgcca
     5101 accgcgcgtc cttgagtgtt gttcggcaac gcttcaacta acttcaaaat ccgcagcgca
     5161 aacagcttcg ttcgttgctt taactcttcc gcatccatga cgtgaggtct cgaatgaatg
     5221 cttcgccttg taacaaatcg gcaatcggca atcgaaaatc ggcaatgcct tactttgtgt
     5281 acttcacggt ttcgcggccc tgctccaaca tctcgcggtt ccagacgaaa ccggcgcgcg
     5341 aatatgtcgc caattcgaaa tcctgtgtca gctccagaca tgaagtcgga cacgcttcct
     5401 gacaaagcgc gcagaacatg cagcgcgaag tatcaaagac aaactcgtcg agcactttct
     5461 ttcggcgcgg cttgcccgcg acgatgatct cgacgtccat cgcaatcacg tcgatacagt
     5521 cttccggaca agcaagcgcg cagagattac aagcaatgca gagcgtgtcg tgcgtttccg
     5581 ggtccatgtt taagcgcgga gctccgtgaa aacgcggagc aacgtccggc cgcacttttg
     5641 gatactgctc ggtatagatt cccttgcccg cgacagcgtc tttgtcagtg atcgcgccaa
     5701 ggttgtattt cagcgtgagc ccgagtcctt tgatcagatc gatcatgaag aaccgtttca
     5761 gcttttgcgc ccacgttggt tttggagtct taactagagg catcgaacta caccttccgt
     5821 ttattgccaa ttgccaattg ccaattgccg attgcggtaa ttgccgattt tcgattgccg
     5881 attttaagaa actcggcgat atctgcgacc cggtactcta gcgaacaatc ggcaattggc
     5941 aatcgaaaat cggcaattac gaaaccgccg gcttgccttt gggcaaattc accaacctga
     6001 tatttcgccg ctgcgacttg agattgaaat cgcgcgaacg ccagttgatg cgcgacatca
     6061 gcagcgcggt gatgaacagc ccggcgacgc cgatcacgat catgatgatt ttgccttgca
     6121 tcgtcagatt caagccgcga tcgataaact cgaccgtctt cattccgcgc cggccatcga
     6181 gcgcctgcac gagaaagatc gccagcgccg acagcgcgat gttgagcagc gccgacggaa
     6241 tcagccactt ccaaccgata tccatcaact ggtcgtaacg aaaacgcggc agtgtccaac
     6301 gcaaccagaa gtagatataa agaataattg aaaccttaac caaaaacacc agcaggctaa
     6361 cgatcacgta caaattgtga taaccgcttt gttccagccg gtagacaaac gggaaatacc
     6421 agccgccgag aaacacggta accgcgacgg ccgcaacgac gaccatcgcc gcgtattcag
     6481 ccatgaaaaa caacgaccag cgcatgccgg aatattcagt gtgaaagccg gcgacaagtt
     6541 ctgactcagc ttccggcaga tcgaacggcg cccgattgtt ctcggcgatg ccgctaatca
     6601 gaaacagaag aaatccggcc ggttgataca agataaacca gattgaatcc gacccctgcg
     6661 cggcaacgat cccggacagc gacaacgtac gcgcaaacat caacgcgccg atcagcgaca
     6721 ggcccatcgc aacttcgtaa gagaccatct gcgcactcga acgcagaccg ccgagtaacg
     6781 cgtatttcga gttcgacgac caacccgcaa gtatcaacgc cagcacgccg acactcgaga
     6841 ccgcgagcac cagcagcaaa ccgatgttta cgtccgcgat caccgcccag tcaggtccaa
     6901 agggaacaac tgaaaagacg atgaaagcag acgccacggc gatgtacggc gcgaagaaga
     6961 agatccaccg atctgcgttc tcgggaatga agtcttcctt agttagcagc ttcagaccgt
     7021 cagcgattgg ttgcaacagt ccccatgggc caacacgcat cggaccgagt cgcgcctgga
     7081 taaacgctga aatcttccgc tcgagatagg tcgcgtaggc tacccacagt gcgaccagcg
     7141 tcacgacgag gccgatctga atcaggggtc agatgaagaa attgatcgtt tcctgactga
     7201 agaacctcag caaaagatcg ttcagaacgt ttattggccc aagaagcaac atgcaggtct
     7261 cagcgatcaa tctctcccaa aacaatatcg agcgtgccaa tgatggccac gacgtcagcc
     7321 accaggtggc cgacgcacat ctgtttcaac gcctgcaaat tcacaaacga cggcgggcga
     7381 acgcgcacgc ggtacggctg tgcgctgccg tcggaaacga ggtaacaacc gatctcacct
     7441 tttggtcctt cgatcgagtg atagtaatcg cctgccggcg gcttcactac tttcggtaac
     7501 ttcgcattca ccggaccatc aggaagattg tcgagcgcct gcacgacgat ccgccgcgac
     7561 tgtctcatct cttccaatcg gacgaggtac cgatcataag tatcgccgtt ttcgccgagc
     7621 gggatatcga agtccatgtc gg
//
LOCUS       DASTEY010000521         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_406966, whole genome shotgun sequence.
ACCESSION   DASTEY010000521 DASTEY010000000
VERSION     DASTEY010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406966"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFO72_09140"
     CDS             complement(<1..421)
                     /locus_tag="VFO72_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9479498.1"
                     /translation="MRKSIGLLTLLFFCSVTACAQHMNVNHHSAPPAESLETQDWAKQ
                     RLAKSSRHQEWVKVKNGEREVSSFVVYPEVKTKATAVVVIHEIFGMTDWVQSLTDQLA
                     EAGYIAIAPDLLSGMGPNGGGTSSLDRTAIGQKIRDLP"
     gene            complement(462..2036)
                     /locus_tag="VFO72_09145"
     CDS             complement(462..2036)
                     /locus_tag="VFO72_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9479499.1"
                     /translation="MFRQLVLLVLLPLTVCAQTTQEKVRDYRRANEHRIIKEYLALLS
                     LPNVASDIQNIRKNANFIAEMMKQRGLSPRLLEGPTRDTPPAVYAEWKVPGAQRTLLL
                     YAHYDGQPTDPKQWSGTHPWQPVFRSAALDAGGEIEAAPAEGTPFNPQSRIYARSASD
                     DKAGVMVILNAFAALKAAGVPLTSNIKFFFEGEEEAGSPHLAEIIKAHRELLAADAWI
                     ICDGPVHQSGKKQVVFGARGDVNVDLTVYAAKRPLHSGHYGNWSPNPAMMLARLLASM
                     KDADDRVTIDGWYSDVEALGERERRAIAEAPQYDDELKKQLGLSRTEGGGKSLMELIN
                     VPSLNINGFASGDVGELARNVIPTTATAVLDLRLVKGNDHRRQVERLIAHVRKQGYYV
                     TQQPPTDAERLQHPLIAGVNVRPGGYNADRTSMELPVSLAVIEAVQSTTPDKIVLLPT
                     SGGSLPLSIITENLKTVTISVPIANYDNNQHAENENVRVQNLWDGIETFAALMTMGHG
                     PDGTRNRNTQKTNHSH"
     gene            2174..3460
                     /locus_tag="VFO72_09150"
     CDS             2174..3460
                     /locus_tag="VFO72_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479500.1"
                     /translation="MKSKFSVLSLLLFVLFVTPTSFATELVDKAVSEKGAESAAAIDE
                     LRSQGPAGLNALMKRYADEIKKHIDDPTLKPNAEWLRITNALDTVSGQKNSFISGLYW
                     YTDLKEAQQAAKESGKPILSLRLLGKLTDELSCANSRFFRTVLYSNAEIASVLRDRFV
                     LHWQSVRPAPIVTIDFGDGRKLERTVTGNSIHYVLDSSGRLLEALPGLYGPKAFLRNL
                     TESESLFKSLIGKSNMAMDSLVAAHHQVRYNTLSAAWLTDTAKIGGKIPEGFSVVRDQ
                     KGQLTAVLVAPLAITKMATERSTLRSMSATSDALSRITDEAAWRKIAQLHASDAMLDS
                     RSIALIKRQNPALSDREFAMMLQKFQELIALDTVRNEYLLHMKLYPWLMGDPRIDLEQ
                     FNEKVYAELFLTPRSDPWLGLLESDVYMGIDNGGVQ"
BASE COUNT          727 a         1000 c          990 g          814 t
ORIGIN      
        1 gcggcaggtc gcggatcttc tggccaatcg cggtgcgatc gagcgaactt gtgccgccgc
       61 cgttcgggcc catgcccgac agcagatcgg gcgcgatcgc gatgtaaccg gcttccgcaa
      121 gttggtcggt aagactctgc acccaatcag tcatgccaaa gatttcgtga atcactacaa
      181 ccgcggtcgc cttggttttt acttccggat agaccacaaa actgcttact tcacgctcgc
      241 catttttcac tttcacccac tcctgatggc gtgaagattt tgccagtcgc tgcttggccc
      301 agtcctgcgt ctccagcgac tccgcaggcg gggcactgtg atggtttacg ttcatgtgct
      361 gcgcgcaggc agtgactgaa caaaagaaga gcaacgtcaa cagaccgatc gattttctca
      421 ttgttgtgtc ccctcaattt ttacttcaac gtcaaccgcg tttagtgcga gtgattcgtc
      481 ttttgtgtat tgcgatttct ggttccgtcc ggtccgtggc ccatcgtcat caaggccgca
      541 aaggtctcga tgccatccca cagattctgc acgcgaacgt tttcattctc agcatgctga
      601 ttgttgtcgt agttcgcaat cggcactgag attgttaccg tcttgaggtt ttcggtaatg
      661 atcgaaagcg gcaggctgcc accggaagtc ggcagcaaca cgatcttgtc gggcgtggtc
      721 gattgcacag cttcgatcac cgcaagcgac accggcaact ccatgcttgt ccggtcggcg
      781 ttgtaaccgc cgggccgaac gtttacaccc gcgattaaag ggtgctgcaa tcgttccgcg
      841 tcggttggcg gttgttgagt gacgtagtat ccctgtttcc gaacgtgcgc gatcagtcgc
      901 tcgacctgcc gccgatgatc gtttcccttc acgagccgca gatcgagcac ggccgtagca
      961 gtggttggaa tcacgttgcg cgctagttcg ccgacgtccc cgcttgcgaa accattgatg
     1021 tttagcgacg ggacattgat cagttccatc agcgacttac cgccgccttc agtacgactc
     1081 aaccccaact gcttcttcag ctcgtcatca tattgcggcg cttcggcgat cgcacgacgt
     1141 tcacgttcgc ctagagcttc gacgtctgaa taccagccgt cgatcgtgac gcggtcgtcc
     1201 gcgtctttca tggaagcgag tagacgcgca agcatcatcg cgggattcgg tgaccagttt
     1261 ccgtagtgac cactgtgcaa cggccgcttc gctgcgtaaa cagtgagatc aacgttcaca
     1321 tcgccgcgcg cgccgaaaac gacctgcttt ttgcctgact gatgcactgg tccatcgcag
     1381 atgatccacg cgtcggctgc gagcaactcg cggtgcgctt tgatgatctc ggcgaggtgt
     1441 ggtgaaccag cctcctcttc gccttcgaaa aagaacttga tgtttgaggt gagcggcaca
     1501 cctgccgctt tcagtgcggc gaaggcgttc aggattacca tcacaccggc cttgtcgtcg
     1561 gaggcggaac gcgcgtagat acgcgactgc ggattgaacg gcgtgccttc tgcaggagcg
     1621 gcctcaatct ccccgcctgc gtccaacgcc gccgaacgaa acaccggttg ccacggatga
     1681 gttccggacc actgcttggg atcggttggc tggccgtcgt agtgggcgta gagcagcagc
     1741 gtgcgctgcg cgccgggcac cttccattcc gcgtaaacag cgggcggcgt gtcgcgcgtc
     1801 ggcccttcca gcagacgtgg actcaggccg cgctgcttca tcatttcggc gatgaagttc
     1861 gcgtttttac gaatgttctg gatgtcgctc gcgacgttcg gaagcgaaag cagggccaga
     1921 tattccttga tgatccggtg ttcgttggcg cggcggtagt cgcgcacctt ttcctgagtg
     1981 gtctgagcac agactgtcag gggcaacagg acgaggagaa caagttggcg aaacatgggc
     2041 ggattatccg acacaaatgt tgtgctggac aaccaaactc acggctgcag gctcaaagaa
     2101 ctactcttcc cgtactgcca atccaaagct caggcatgaa tgcctgtttg ccaaattccg
     2161 cggaggtttc ccgatgaagt cgaagttctc cgtcctttcg ttactcctgt ttgttttgtt
     2221 cgtaactccc acgtcgttcg ctactgaact tgtcgataaa gccgtttcgg aaaaaggcgc
     2281 cgaatctgct gctgccatcg acgagctgcg tagccaaggg ccagcggggt tgaacgcgtt
     2341 gatgaagcgg tacgctgatg aaattaagaa gcacattgac gaccctaccc tcaagccgaa
     2401 tgcagaatgg ctacgtatca ccaatgcact cgacaccgtc agcggccaaa agaacagttt
     2461 tatttccggt ctctactggt acaccgatct gaaagaagcg cagcaggccg cgaaagaaag
     2521 cggcaagccg attctgtcgc tgcggttgct gggcaaactc actgacgaac tgagctgcgc
     2581 aaacagccgt ttctttcgca cggtgcttta ctccaacgcg gagatcgctt ccgttttgcg
     2641 cgatcggttc gtgcttcact ggcagtcagt acgtcccgcg ccgatcgtga cgatcgattt
     2701 cggcgatggc cgcaagctcg aacgcacggt caccggcaac agtattcatt acgtactcga
     2761 ttcgtcaggc cgcttgctcg aagcgttgcc tggactctat ggtccaaaag cgttccttcg
     2821 caatctgact gagtcggaga gtttgttcaa atcgctgatc ggcaagagca acatggcgat
     2881 ggattcgctg gtcgcggctc accaccaggt tcgctacaac acgctttcgg ctgcgtggct
     2941 cacggatacc gcgaaaatcg gcggcaagat accggagggc ttctctgtcg tgagggacca
     3001 aaagggccaa ctgacggccg ttttggtagc tccgctggcg ataacgaaga tggcgactga
     3061 gaggagtacg ctgcggtcga tgtcggcaac tagcgacgcg ctgagcagaa ttacggatga
     3121 agcggcgtgg cgaaagatcg cgcagttgca tgcgtcagat gccatgctcg atagccgcag
     3181 catcgcgctg attaaacgtc agaaccccgc gttatccgac cgtgagttcg cgatgatgtt
     3241 gcagaagttt caggagttga tcgcgctcga caccgtgcgc aacgaatacc tcttgcacat
     3301 gaaactctat ccatggctca tgggcgatcc tcgcatcgat ctcgagcagt tcaacgagaa
     3361 ggtttacgcg gagttgtttc tcactccccg atcagatccg tggttaggac tcttggagag
     3421 cgatgtttac atgggaattg ataatggtgg cgttcaatag gaacggatca cttcttcatc
     3481 aattccagca cggctgaagt catcgccttc acacccgtcc ggagcgttgg c
//
LOCUS       DASTEY010000522         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1000660, whole genome shotgun sequence.
ACCESSION   DASTEY010000522 DASTEY010000000
VERSION     DASTEY010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000660"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFO72_09155"
     CDS             <1..192
                     /locus_tag="VFO72_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9479501.1"
                     /translation="NGPDVPTPVTDGKYFYIVNDRGIVWCLDAKTGAEVYGQQRIKPG
                     TYSGSPVLADGKIYITNED"
     gene            341..1945
                     /locus_tag="VFO72_09160"
     CDS             341..1945
                     /locus_tag="VFO72_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479502.1"
                     /translation="MLRSTLLTFLLVVTANAQESKLGRVEFPTSGSPAAQGHFLRGLA
                     ALHSFWYEEALEAFRESTKADPDFMMGYWGEAMAHNHPLWSEQDLNAAREVIARIKDT
                     PKLTDRERAYLNAVKLLFGEGDKRARDAAYSAAMEKIYGAYPEDLDAAAFYSLSLLAL
                     VGPGEKGYRLQARAGAIALAVYEKNPNHPGAAHYIIHAFDDPDHAILALPAARRYADI
                     APEAHHARHMPSHIFLQLGMWPEAAASNESAWESSDNWMKRKKLSLSVRDYHSLHWLL
                     YAYLQQGRYSKAEQLLKLMKQVMSESTYDNKLRPDYYENNYANMAAAFIVETERWDLA
                     NELFPVDKSPAPSGQISSTGHNMHGSTPADTVRYSSASRTVPAFIRALSAAVRGSNDV
                     PSGLTEIRDLQVSAVVASMKNDHAKAIELMKKATALEEQSSPPSGPPSLIKPTHELFG
                     ELLLRAGKPAEAAAQFRLALLRQPNRARSLLGAARAAAQSGDQAGAAAAYTKFIEQWN
                     QADEGLAELREARSYVKGRPQITRTN"
     gene            complement(1949..>3049)
                     /locus_tag="VFO72_09165"
     CDS             complement(1949..>3049)
                     /locus_tag="VFO72_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9479503.1"
                     /translation="ETGLLKQWPAEGPPLVWKASGAGRGYSSFSVANGRLYTMGLRGD
                     REFVIAFDIANGKEAWATPHGNAFRNDRGDGPRGTPTIDGDRLYALGGNGDLSAMDAR
                     TGRIIWTKNVLREFGGSNITWGISESPLVLGNKVLVNAGGRNASIVALNKENGTLIWK
                     SQSDEAGYSSAIPLQVNGGTQVIFFTGERAVGLDVNDGRLLWEYSRPSNRTANVATPI
                     ARGNRVFISSDYGTGGGVVEIKADGKAEEIYFTKDMRNHHSSSVLVGDHLYGFSSAVL
                     TAMRFDTGEIAWRDRSVGKGSLVYADGHLYCFSENGVVGLVEATPAGYREKGRFRIPQ
                     DSLPTWSHPVVAGGRLYLRDQDTIYAFDVREK"
BASE COUNT          698 a          916 c          821 g          614 t
ORIGIN      
        1 aacgggcctg acgtgccgac gccggttacg gacggaaagt atttttacat tgtgaacgat
       61 cgcggcatcg tatggtgtct cgacgccaaa acgggtgcgg aggtttacgg acagcaacgc
      121 atcaagccgg gcacttacag cggctcgccc gtgctggccg acggcaagat ctacatcacg
      181 aatgaagact gactgacgac tgtgctcgcg gctggtccaa agtttgaggt actggcggag
      241 aacgcgctga acgactacac tctgagttca ccggcaattt ccgatggacg gatttacatc
      301 cgtacctcag gttttctcta tagcattggg gaaaaatagc atgctccgct caacgcttct
      361 aacgtttctc ctggttgtaa cggcgaacgc gcaggagtcg aagctgggtc gcgtcgagtt
      421 tccgacatcc ggctcacccg ccgcgcaagg gcacttcctt cgtggtctcg ctgcgcttca
      481 ctcgttctgg tacgaagaag cgctcgaggc ctttcgcgag tcgactaaag cggatcccga
      541 tttcatgatg ggctactggg gcgaagcgat ggcccacaac catccgctct ggtccgaaca
      601 ggatctcaac gctgcacgcg aggtcattgc caggatcaaa gatacgccca agcttaccga
      661 tcgtgaacgc gcttacctga acgccgtgaa attgctattc ggcgaaggcg acaaacgagc
      721 gcgagacgcc gcttactccg cagcaatgga gaagatctac ggcgcgtatc ccgaagatct
      781 cgatgcagcg gcgttctatt cgttgtcgtt actcgccctg gttggtccgg gtgaaaaggg
      841 ttatcgctta caggcacggg caggagctat tgccctcgcg gtctacgaga aaaatccaaa
      901 ccatccgggc gcggcgcatt acatcattca cgcgttcgac gatccggatc acgccattct
      961 cgcgttacct gctgcacggc gttacgctga catcgcgccc gaagcgcatc acgcgcgcca
     1021 catgccgtcg cacatttttc tgcaactagg gatgtggcct gaagcagcgg cgtccaatga
     1081 atcagcctgg gagtcgtccg acaactggat gaagcgaaag aagctgtcgc tcagtgtgcg
     1141 cgactatcac agtctgcatt ggctgctcta cgcgtatctg caacaaggcc gttacagcaa
     1201 agccgagcag ttactgaagc tgatgaagca agtgatgtcg gaatcgactt acgacaacaa
     1261 gttgcggcct gactattacg aaaacaacta cgccaacatg gccgcggcgt tcattgtcga
     1321 aacagaacga tgggatctcg cgaacgaact ttttccagtg gataagagtc cagcaccttc
     1381 tggtcagata tcgagtacag gtcataatat gcacggctca acaccggcag acaccgtccg
     1441 gtattcaagt gccagccgca ctgtgcctgc ctttatccgt gctttgtccg ccgcagtccg
     1501 tggctcaaac gacgttccct ctggtctgac cgagatcaga gacctgcaag tctcagcagt
     1561 ggtcgcctcg atgaaaaacg atcacgccaa agcgatcgag ttgatgaaaa aagccacggc
     1621 actcgaagaa caatccagtc cgccatccgg accgccgtca ctaatcaaac caacacacga
     1681 actattcggc gagcttctgc ttcgcgcagg taaaccggcc gaagcggcag cgcaattcag
     1741 actggcgttg ctgagacaac cgaatcgcgc acgctcattg ctcggtgccg cacgcgccgc
     1801 cgcacaaagc ggcgatcaag ctggcgcagc ggccgcgtat acaaaattca tcgagcagtg
     1861 gaatcaggca gacgaaggac tcgctgaatt gcgcgaggcc cgcagttacg tcaaaggcag
     1921 accacagatt acacggacta attaacggct acttctcgcg cacgtcgaac gcgtagatcg
     1981 tgtcctgatc gcgcagatac aaccgcccgc ccgcaacaac gggatggctc catgtcggca
     2041 gtgaatcctg cgggatgcgg aagcgtcctt tctcgcgata acccgccggc gttgcttcga
     2101 ccagtccgac gacaccgttc tcactgaaac aataaagatg tccatcggcg taaacgagcg
     2161 agcctttgcc tacgcttcga tctcgccatg cgatctcacc cgtgtcgaac cgcatagcag
     2221 tcaggaccgc gctcgagaaa ccatacaaat gatctccgac gagaacggaa ctgctgtgat
     2281 ggttacgcat gtctttcgta aagtagatct cctccgcttt gccgtccgct ttgatctcga
     2341 cgacgccgcc tcccgttcca tagtcggaag agataaagac gcgattgcca cgcgcaattg
     2401 gcgtcgcgac gttggctgtt ctatttgacg ggcgactgta ctcccacagt aaacgcccat
     2461 cgttcacgtc gagaccgaca gctcgctcgc cagtgaaaaa gatgacctgc gtaccgccat
     2521 taacctggag cgggatcgcc gaagagtaac cggcttcatc gctctggctc ttccaaatca
     2581 acgtgccgtt ctctttgttc agtgcaacaa tggacgcgtt gcggccgccg gcattcacca
     2641 gcaccttgtt gcccaggacc agcggcgact cgctgatgcc ccacgtaata ttcgaaccgc
     2701 caaactctcg cagtacgttc ttggtccaga tgatgcggcc ggtacgcgcg tccatcgctg
     2761 aaaggtcgcc gttgccgccg agcgcgtaga gacgatcgcc gtcgatggtc ggcgtgcctc
     2821 ttggaccgtc gccacgatcg ttgcgaaatg cgttgccgtg cggggtggcc catgcttctt
     2881 ttccgttggc gatatcgaac gcaattacaa actcgcggtc gccgcgcaaa cccatcgtat
     2941 acaagcgacc gttagcaacc gaaaacgacg agtaaccacg tcccgcgccg gacgctttcc
     3001 agaccaacgg cggaccttcc gccggccatt gcttgagcaa tcctgtctc
//
LOCUS       DASTEY010000523         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_440188, whole genome shotgun sequence.
ACCESSION   DASTEY010000523 DASTEY010000000
VERSION     DASTEY010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440188"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..549)
                     /gene="mreD"
                     /locus_tag="VFO72_09170"
     CDS             complement(7..549)
                     /gene="mreD"
                     /locus_tag="VFO72_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET9479504.1"
                     /translation="MSQLKIAVVLLVSILLQLLLREVWRPLSYIDFPLVVVVYIALQR
                     DAWQALLVGNLAGLIVDAASGGLIGAGGFSKTLTAYVIYFAATRVNLENPLLRIPVLA
                     AGSLLDSAIYVFWHRVLGYPPAVPFVQTMSYKLIATTVAGTLALYIIDALVAERSAQR
                     RQFATRRRVARRSTGPLRRR"
     gene            complement(568..1473)
                     /gene="mreC"
                     /locus_tag="VFO72_09175"
     CDS             complement(568..1473)
                     /gene="mreC"
                     /locus_tag="VFO72_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET9479505.1"
                     /translation="MASVRTQKEIRQRAPIWLVTLLVANLVIMAVDARDADGQHKVLR
                     IWTQTFASPLQSASSKASGATTGFFQQIWNFRSTAQENEQLKERLTQVETELNNARQA
                     AVENERLKGLLGLSEQTDIKSVPARVIARDPSVWFNTITINRGSSSGIQMNMPVVTPG
                     GIVGRVITVSPFASQVMLLTDEKAGAGAVVGQLGQSGALGSVRGRADLGVALIEMRYV
                     SGTEKVEVGDNVMTTGQDGIYPAGLNVGRVVDVKSGTATQAHQILIQPGAQLDRLEDV
                     AVLLYHPPPRSVPEQTPQNADRSRR"
     gene            complement(1520..2566)
                     /locus_tag="VFO72_09180"
     CDS             complement(1520..2566)
                     /locus_tag="VFO72_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9479506.1"
                     /translation="MAFKSIFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     VTNQVEAVGRDAKDMLGRTPGNIVAIRPMKDGVIANFEVTEKMLQHFIRKAHNGKTWV
                     SPRVVIGIPSEITQVERRAVEDSAYRAKASEVYLVEEAMAAAIGADLPITEPHGNMVV
                     DIGGGTTDIAVISLSGIVYSRAVRVAGNEMDEAIIQYIKRKYNLLIGERTAEAIKIEL
                     GSAYPLDEPLSYEVRGRNLIEGIPKTITITDEEVREALADSISTIINAVRVALERTPP
                     ELSADIVERGIVLTGGGALLKNLDKRLSIETGLPVSLADDPLASVVLGTGKMLSDFDL
                     LKRVKWENTMTSGM"
BASE COUNT          616 a          786 c          754 g          638 t
ORIGIN      
        1 agcacttcac ctcctcctca gcggtcccgt cgaacgtcga gcaacgcgac gacgagttgc
       61 gaattgacgc cgttgtgccg atcgctccgc gaccagcgca tcgattatgt agagcgccag
      121 cgttccggcg acggtcgtgg caattagttt atatgacatt gtctgcacga acggaacggc
      181 cggtggatac cccagcaccc ggtgccagaa aacgtagatt gccgaatcga gcaacgaacc
      241 cgcggcgagc accggaattc tcaacagcgg gttttcgaga tttacgcgcg tcgccgcgaa
      301 atagatgaca tacgccgtca gagtcttgga aaaacctcct gcgccaatca agccaccgct
      361 ggcagcatca acgatcaatc ctgcgaggtt cccgaccagc aacgcctgcc aggcgtcacg
      421 ttgcaaggcg atgtaaacaa caacaacgag gggaaagtcg atataagaga gtgggcgcca
      481 aacctcgcgc agcaaaagct ggagcaggat agagactaac agaactacgg cgatcttaag
      541 ctgcgacata gaaagatggc aatcgcttca tctcctggat ctgtctgcgt tctgcggtgt
      601 ctgttcgggg actgaacgtg gcggggggtg atataaaagc acagcaacgt cttcaaggcg
      661 atcgagctgg gcgccgggtt gaatgagaat ctgatgggcc tgcgtggcgg ttccgctttt
      721 tacgtcaaca actcggccca cgttcaaacc agccggataa attccgtcct gaccggtcgt
      781 catcacgttg tcgccgactt caactttctc cgtacccgac acgtaacgca tttcgatcag
      841 cgccactccg agatcggccc ggccacgcac cgaaccgagc gcgcccgatt gtccgagttg
      901 accgacaacg gcgccggcgc ctgctttttc atcggtgagc aacatgacct gggacgcaaa
      961 gggactaacg gtgatgacgc gccccacgat tccgccggga gttaccacag gcatgttcat
     1021 ttggattcct gaactactgc cgcgattgat cgtgatcgtg ttgaaccaca ctgaaggatc
     1081 tcgcgcgatg acgcgcgcgg gaacactctt aatatcggtt tgttcgctga ggccaagcag
     1141 tcccttgagc cgttcgtttt cgactgccgc ctggcgcgcg ttattgagtt ccgtttcaac
     1201 ctgcgtcagg cgttctttta gttgttcgtt ttcctgcgcc gtgctgcgga agttccagat
     1261 ttgctggaag aacccggtgg tggcgccgct ggcttttgaa gatgcactct gcagcggtga
     1321 agcaaaggtt tgcgtccaga tgcggagtac tttgtgttgg ccatcggcgt cgcgcgcgtc
     1381 tactgccatg atgacgagat tggcgacgag caacgtcacg agccagatgg gggctcgctg
     1441 tcgaatctct ttttgtgtgc gaaccgaggc cacgcagtaa atctcaaatc gacagaaaac
     1501 acagatagat ccgtgttatc tacattccgc ttgtcattgt gttttcccac ttcacccgct
     1561 tgagcagatc gaagtcggac aacattttgc cagtacctaa cactacggag gcaagtgggt
     1621 cgtcggccag cgaaaccggc aatccggttt cgattgaaag ccgcttgtcg agattcttca
     1681 ataaagcgcc gccgcccgtc agtacgatgc cacgctcgac aatgtctgct gacagttcgg
     1741 gaggcgttcg ttccagggcc acgcgcaccg cgttgatgat cgtcgagatc gaatccgcca
     1801 gagcttcgcg cacttcttcg tcagtgatcg tgatcgtctt cgggattccc tcgatgaggt
     1861 tacgaccacg cacttcgtac gacagcggtt cgtcgagcgg gtacgccgag ccgagctcga
     1921 tcttgattgc ctcggctgtt cgttcaccaa ttagcaagtt gtacttcctc ttgatgtact
     1981 ggatgatcgc ttcatccatc tcgtttcccg cgacgcgcac cgcgcgcgag tacacgatac
     2041 cgcttaagct gatcacagcg atatccgtcg tgccgccgcc gatgtcaacg accatgtttc
     2101 cgtgcggctc ggtaatcggt aagtcggcgc cgatcgccgc tgccattgct tcttcgacca
     2161 gatagacctc ggaagctttg gcccgataag ccgagtcttc cacggcacgg cgttcgactt
     2221 gagttatctc agaaggaata ccgatgacga ctcttgggct aacccaggtt ttgccgttat
     2281 gcgccttgcg gatgaagtgc tgaagcatct tctccgtgac ttcgaagttt gcgatcacac
     2341 cgtccttcat cggtcgaatg gcaacgatgt ttccgggagt tcggcccaac atgtccttgg
     2401 cgtcacggcc aacagcttca acctgattag ttactttatt aatggctacg atagaaggtt
     2461 ctgaaacgac gatcccgcgt cccttcgcat agaccagcgt attcgcggtc cccagatcaa
     2521 tagcaaggtc actggaaaac agactgaaaa tagatttgaa agccatttag acttattcac
     2581 ttcccgcgga tgtagaatgg aacttcgggt gggagcggcg gtaaatttcg ccaaacacgt
     2641 ccgaaatcct aagatacacc ctcacgtact aaccgtaaac cggaaacttc aaccacagat
     2701 tacacggatg tagacggatt agctcggcag atccgtgtac tctgtcgttt ccgcatttac
     2761 tattgcgcgt ctggtttgtt ctcggggggt taag
//
LOCUS       DASTEY010000524         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_341306, whole genome shotgun sequence.
ACCESSION   DASTEY010000524 DASTEY010000000
VERSION     DASTEY010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341306"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="thyA"
                     /locus_tag="VFO72_09185"
     CDS             <1..459
                     /gene="thyA"
                     /locus_tag="VFO72_09185"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR03284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET9479507.1"
                     /translation="RTNPDSRRLIVTGWDPRQADNVDLPPCHTLFQFKVEDGRVLHCQ
                     LYQRSADAFLGVPFNISSYALLMHLVAHVSGLSAGEFVYTLGDYHIYQNHRAQVDELL
                     SREPLPLPQLQINDPDNALRGLEGLLAMRYEHLNLIGYKSHSKIAAPVAV"
     gene            463..936
                     /locus_tag="VFO72_09190"
     CDS             463..936
                     /locus_tag="VFO72_09190"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET9479508.1"
                     /translation="MIIGIAAVDRQGAIGKGGKLPWHYSADMKFFRQTTTGHAVVMGR
                     KTWMTLGKPLKNRLNIVLSRDSSIEPQESLLVLSDIDSVLSLNDSLATDLFVIGGAQI
                     YAAFLPRIERWIITHVPLKVDGADAFMPQGYLDGFKEADSKQIGEGLTVKYYDRT"
     gene            complement(933..2291)
                     /gene="radA"
                     /locus_tag="VFO72_09195"
     CDS             complement(933..2291)
                     /gene="radA"
                     /locus_tag="VFO72_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET9479509.1"
                     /translation="MKKTETLFVCQSCGHKSGKWLGKCPECGEWNSLVEERNQSTRGG
                     RNGFQLREVTAVAYAEIESQDDRRLPSGVTEFDRVLGGGIVPGTLALIGGDPGIGKST
                     LLLQVADKLSDSGTVVLYVSGEESERQIKLRGERLAVTAKNVFLLPETNLENIFREIE
                     RLKPGAVIVDSIQTVFSSVIESAPGSVSQVREVAHQFLLLAKNRGMPVFLIGHITKEG
                     SIAGPTALEHIVDTVLYFEGERHHNHRIVRAVKNRYGAANEVGVFEMTGRGLMPVANP
                     SQMFLQERPQNVAGSIVTACMEGTRPLLVEIQALVSGNKYGTGRRMTQGLDQNRVALL
                     IAMLEKRAGLQLAGDDVFVNIAGGLEVDEPAADLGVVTAIASSFKNMTVDPHTAVFGE
                     VGLTGEVRGAMQASVRAREAQALGFKKIVMPASNVAGLERLLGVRVVGVRSVDEALEE
                     LF"
     gene            complement(2306..2605)
                     /locus_tag="VFO72_09200"
     CDS             complement(2306..2605)
                     /locus_tag="VFO72_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HET9479510.1"
                     /translation="MDKLLRESGVPFEKVNYYVEPLSKKKLTELIRKLNLKPRELLRK
                     SEAVYKELGLASGEFTDSELIALMVEHPDLLQRPIVERGDRAVLGRPTENVRALL"
BASE COUNT          570 a          768 c          732 g          629 t
ORIGIN      
        1 cgtacgaatc ccgattcgcg ccggctaatt gtaacgggat gggatccacg gcaggcagac
       61 aatgttgact tgccgccgtg tcatacactc tttcagttta aggttgaaga tggccgcgtg
      121 cttcattgcc agctttacca acggtcggca gatgcctttc tcggcgtgcc tttcaacatc
      181 agctcatacg ctttgctcat gcacctcgtc gcgcacgtgt ctggattgag cgccggcgag
      241 tttgtttaca cgttgggtga ctatcacatc tatcaaaacc acagggcgca agtggacgag
      301 ttgttgtcgc gcgagccgtt gccgttgccg cagctgcaga ttaacgatcc tgataatgct
      361 ttacgtggac tcgaaggttt attggctatg aggtacgagc acctgaactt aattggttac
      421 aaatcgcata gcaagatcgc agctcccgtc gccgtctaga tcatgattat cggcattgca
      481 gcagtcgatc gccagggtgc gattggaaaa ggcggaaagc ttccgtggca ctattctgcc
      541 gacatgaagt tttttcgtca gaccacgacg gggcacgcgg tcgtgatggg acgtaagacg
      601 tggatgacgt tgggcaagcc gttaaagaac agactcaata tcgtgttgag tcgcgattcg
      661 agtattgagc cgcaggagtc gttgctcgtg ttgagcgaca tcgattcggt gctgtcactg
      721 aacgactcgc tggcgaccga tctgtttgtg attgggggcg cgcagattta tgcggcgttt
      781 ctgccgcgga ttgaacggtg gattatcacg catgtgccgc tgaaagtaga cggagcggat
      841 gcgtttatgc cgcagggtta tctcgatggc ttcaaagaag ccgattcaaa acagatagga
      901 gagggattaa cagttaaata ctacgaccgt acttagaaca actcctctaa agcttcatcc
      961 acagaccgca caccaacaac acggacaccc agcaaccgtt ccaatcctgc gacattcgac
     1021 gcgggcatca cgatcttctt gaagcccagc gcctgcgcct cccgcgctcg taccgaagct
     1081 tgcattgcac cgcggacctc gcctgtcagt cccacttcac cgaataccgc cgtgtgtgga
     1141 tcgaccgtca tgttcttgaa actcgaagcg atcgccgtca cgacgccgag atcggcagcg
     1201 ggttcatcga cttcgagacc gcccgcgatg ttcacaaaca cgtcgtcgcc tgctagttgc
     1261 aatccggcgc gtttctcgag catcgcgatc agcagtgcga ctcgattctg atcgaggccc
     1321 tgcgtcatgc gccggcccgt gccgtactta tttcctgaaa ccagcgcctg aatctccacc
     1381 aacaacgggc gcgtaccttc catgcaggcg gtcacaatcg acccggcgac gttctgcggc
     1441 cgctcctgaa gaaacatttg cgacggattc gccaccggca tcaacccgcg accggtcatc
     1501 tcgaaaacgc cgacctcgtt cgcggcgccg taacgattct tgaccgcgcg tacgatgcgg
     1561 tggttgtgat gtcgttcgcc ttcgaaataa agaaccgtat cgacgatgtg ttcgagcgcc
     1621 gttggaccag cgatcgagcc ttccttggtg atgtggccga ttaggaaaac gggcatgccc
     1681 cggttctttg cgagcagtag aaactgatgc gccacttccc tcacctgcga caccgatccg
     1741 ggtgctgact cgatcacact cgagaacact gtttgaattg agtcgacgat taccgcgccg
     1801 ggcttgaggc gttcgatctc acgaaagatg ttttcgagat ttgtttcggg gaggagaaag
     1861 acgtttttcg ccgtgactgc gagacgttcg ccgcgaagtt tgatctggcg ttcggattct
     1921 tcgccggaga cgtagaggac gacggtgccg gagtcgctga gtttgtcggc gacttgaagc
     1981 agcagagtgc tcttaccaat gccgggatcg ccgccgatca aagcgagcgt tcctggaact
     2041 atgccaccgc cgaggacgcg atcgaattca gtgacgccgg acggcagacg gcggtcgtcc
     2101 tgcgattcga tctcagcgta agcgaccgcg gttacctcgc gtaattggaa tccgtttcgg
     2161 ccgccgcgtg tcgattggtt ccgttcttcg acgagtgaat tccactcgcc acattccgga
     2221 cacttgccca gccacttgcc ggacttgtgc ccacagcttt ggcagacgaa tagcgtttca
     2281 gtttttttca cggataaaac ggattctaca acaaagctct aacattttcg gtgggccggc
     2341 cgagcacggc gcggtcgccg cgttcaacga ttggacgctg caacagatcg gggtgctcga
     2401 ccatcagggc aattaattcg ctgtcggtga attcaccact cgccagcccc aactccttat
     2461 aaaccgcctc actcttgcgc aataactcgc gcggcttcaa gttcagcttg cgaatgagct
     2521 ccgtgagttt cttcttgctc aaaggctcga cgtagtaatt caccttttca aacggaacac
     2581 cgctctcccg gagcagcttg tccatctcgc gacacttcgt tcaagtcggt ttctcgtaga
     2641 ctgtgatctt gtccgccata atttacggga aaatccccct cacgcttgtt gcttccgcc
//
LOCUS       DASTEY010000525         7568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_259114, whole genome shotgun sequence.
ACCESSION   DASTEY010000525 DASTEY010000000
VERSION     DASTEY010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259114"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VFO72_09205"
     CDS             complement(<1..1329)
                     /locus_tag="VFO72_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9479511.1"
                     /translation="MLERRLGQGGMGIVFQARHIFLKTSHAIKVILPDLVGNDPMLTT
                     RFRQEALAAAAIRHQNIIAVTDFGVARGTMPFLVMEFVKGRSLQEIMTKEGAMPVQRA
                     WELITAIAAGVGAAHRQNIVHRDLKPLNIMVQDDVPTAEGVKILDFGLAKIKSGELLG
                     SFIQAQTSGLMGSPFYMAPEQWSDEEPDARADIYSLGVILFQMLSGEVPFKGSSIPSI
                     MKKHLTMPPPTFQSMGVSVPPAIEAVVRHALEKEVDARIDSIDTFVKELHAAINSTPF
                     VASLRETVAMDPNRTMASAVTTLNPPLESPGAKTQPPVNNDTQFAASFELLDGTISGT
                     DLAPEVQPQAPEVHDPETTHVQRRPAPPSVPVQPSKPVWQTIDFDKRRRVSPLWFIGA
                     AILVVVVAGLGISVFLFLTSKQSTDDPGPTPAPTIAAGTKADLVAIEGGSF"
     gene            complement(1472..4021)
                     /gene="leuS"
                     /locus_tag="VFO72_09210"
     CDS             complement(1472..4021)
                     /gene="leuS"
                     /locus_tag="VFO72_09210"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET9479512.1"
                     /translation="MDEKYFPEQIEKKWQQRWAESGVLQAKEEDPREKFYALEMLPYP
                     SGFLHMGHVRNYSIGDALAWFKRLQGFNVLHPIGWDSFGQPAEQAAIKRGVNPRDWTE
                     ENISHMRGQLQRLGISYDWSREIAAHRPDYYKWDQWFFLKMLERGLAYKKVSQVNWCP
                     KEETVLSNEQSSGGICWRCGTQVEKRDLEQWFLRITDYAEQLVADIKQIEAGWPEKVL
                     KRQSDWVGRSEGAYVDFPVKDSDKKIRVFTTRIDTIYGANAVVLSAEHPLLDELLEGS
                     ALKADIQAFAEKVRQQRATRGDEIEEAKEGLNTGVLAINPFSGENLPVWVANYVLMEY
                     GTGAVMSVPGHDERDFEFAQKYSLPIRQVIAPVTHEQVGAEPVATTAGDASAEMRESF
                     TGYGVLINSGEWGGKLSEVAVKEMAEYAKRHGFGEAAVTYRIRDWGISRQRFWGAPVP
                     VIYCPNCGMVPVPYEQLPVVLPQTASFTGTGESPLAGVPDFVNTACPNCESPARRETD
                     TMDTFVDSSWYFFRYLDPQNEDAPFDTGKARRWTPVDQYIGGDSHAVMHLIYTRFWTK
                     FMRDLGLVDFDEPVKRLLTQGMVTNRVEGTDEWKAMSKSLGNGVDPDEMIAAFGADAT
                     RLFVLFAAPAENELRWSESGIEGAVRFLRRVYTMVWRWHERLSRRDSESSSLDFSHGA
                     RTLRRKTHQTIAKITNDFEQLHLNTSVAALMELFNQLSDFNADPATASEADVFAVREA
                     LEALVIMLAPFAPHIAEEMWEGLGHDGGLLSEVPRWPIPEPGLAQREELEIPIQVNGK
                     LRSRLIAAPGVSEDELRNSALADERVRALINGRQVVKVIVVPERLVNVVIK"
     gene            complement(4043..4924)
                     /locus_tag="VFO72_09215"
     CDS             complement(4043..4924)
                     /locus_tag="VFO72_09215"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET9479513.1"
                     /translation="MPRRPKIGFHAPIKGGVHNALIVAKETGCETVQLFSRNPRGWMA
                     KPLTLEDVLLFRKTRRVTKISPVLIHCNYLVNLAAADEVMLQKSRESFREEVERALLL
                     GVDYLVVHPGSSRGACEADGIALCATSLKIACKGLQLGSLRILLENTAGQGDCIGHRF
                     EHLDAIMRACPKLPLGVCMDTAHAFTAGYDIREADGLEALIQRVQKTVGVKNVRAIHF
                     NDSRAVYNSRVDRHWHIGEGHIGADALRRVAQHPKLKHAAFILETPYDDPRADLKNLA
                     MLRSFITDLHTDKHGLL"
     gene            complement(4944..6143)
                     /locus_tag="VFO72_09220"
     CDS             complement(4944..6143)
                     /locus_tag="VFO72_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET9479514.1"
                     /translation="MTAAFLAGCHKTTMNQDNKPMTVAQDSADDPLTIHKRAIAVDMH
                     VDTVQRLLDERIDLQQQLSDGHFDAVRARAGGLDAQFFSIWVEPQLFGGGGERAVKRA
                     DDQIAAIRALADKHPETWQLATSADDIRRAVQDGKIAALMGLEGGYAIDEKLENVQRY
                     YDLGVRYMSPAWSVSTSWAGSSGDEAGATRGLNDFGKSVVREMNRLGMMVDVSHVSDK
                     TFWDVVNTSTKPVVATHSACRALANVPRNLTDDMIRALAKTGGVVNVIFYPEHIEPGW
                     SELKRKADLEIAPMVQDASASEPGGAVRKKMARDRVRQREYARRLPPVYVARVVDHID
                     HIVKLVGVDHVGIGSDFDGILSTAADLATVADLPNLTKELLRRGYSAGDIEKILGGNM
                     LRVMGAQ"
     gene            6294..7337
                     /locus_tag="VFO72_09225"
     CDS             6294..7337
                     /locus_tag="VFO72_09225"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9479515.1"
                     /translation="MFAKDGNPTDVKTPRICDVVWHNGEFIKWDDARVHVMSHLLHYG
                     SSVFEGIRCYATKRGPAVFRLREHMQRFLNSAKIYRMDHPWTLDELCDAVVEIARRSE
                     MDQCYIRPILFRSLDEAHPAFGVNPFPNPLACYIGAWDWGKYLGDEAIEQGVDVCVST
                     WNRLTPNSMPAMAKSGANYMNSQLIKMEALLNGFAEGIALDDRGYVSEGSGENIFLVT
                     GDVVLTPPLSSSILPGITRDSVIQICHELGITIKERTIQRAALYVADEVFFSGTAAEI
                     TPIRSVDRITVGRGGRGPITEQVQKVFFEITKGERKAPGNWLTYVNETAKADRNGFAA
                     GSVDATAELKVSV"
BASE COUNT         1563 a         2286 c         2054 g         1665 t
ORIGIN      
        1 aaaagacccg ccttcgatag cgacgagatc cgcttttgtg ccggcggcga tagtcggcgc
       61 cggcgttggt ccaggatcat ccgtgctctg tttggaagtt aggaacagga aaacgcttat
      121 tccaagtccg gcaacaacca caaccagaat ggcagcgcca ataaaccaga gcggagaaac
      181 tcgccggcgt ttatcaaagt cgattgtctg ccagactggt ttcgacggct ggaccggcac
      241 actgggcgga gcaggcctcc gctgcacgtg cgtcgtttca ggatcgtgaa cctcgggagc
      301 ttgcggctga acctctggcg ccaaatcggt gccgctgatc gttccgtcca gcaactcaaa
      361 cgacgcggca aattgagtgt cgttgtttac cggtggttga gtctttgctc caggagactc
      421 gagcggcgga ttcagcgtag taactgccga agccatcgtt cgattcggat ccatcgcgac
      481 cgtttcacgc aacgatgcca caaacggagt ggagttgatc gcggcgtgaa gctctttaac
      541 aaacgtgtcg atcgagtcga tgcgcgcgtc gacttccttc tccagcgcat gccgcacgac
      601 tgcttcaatc gccggcggca ccgaaacacc catcgattga aaagtcggcg gcggcattgt
      661 cagatgcttc ttcatgatcg acggaatcga agatcctttg aacggcacct caccggagag
      721 catctgaaag agaatcaccc ccagactgta aatgtcggcg cgagcgtccg gttcttcatc
      781 agaccactgc tccggcgcca tataaaaagg cgaacccata agcccgctcg tttgcgcctg
      841 aatgaacgat ccgagcaact cacctgattt gatcttggcg agaccgaaat cgagtatctt
      901 cacgccttcc gcagtcggca catcgtcctg gaccatgatg ttgagcggtt tcagatcgcg
      961 atggacgatg ttctgccggt gcgcagcgcc gactccggct gcgatcgcag tgatcaattc
     1021 ccacgcgcgt tggaccggca tcgcgccttc tttggtcatg atctcctgca aggatctgcc
     1081 tttgacaaac tccatcacca gaaacggcat ggtgccgcgc gcgacgccga agtcggttac
     1141 cgcgatgatg ttctggtgac gaatggctgc ggcggcgagc gcctcctggc gaaaacgcgt
     1201 agtcagcata ggatcgttgc cgaccagatc gggcaggatc actttgatcg cgtgcgatgt
     1261 tttgagaaag atgtgccgcg cctgaaagac gatacccatg ccgccttgcc cgagacgtcg
     1321 ttcgagcaag tagcgggcat cgattgtggg ttcgccaaga agggttggtg cagtcggctc
     1381 accgtcttgg gggcagtgat cggtctcgtc ggggtaacac cgcctgcact tggggcattc
     1441 tttcatttaa tctgcgtcat ctgcggactg actactttat cacaacgttt acgaggcgtt
     1501 cgggaaccac gatcactttc accacctggc gtccattgat caacgctcgc acacgttcat
     1561 ccgccagcgc cgaattgcgc agctcgtctt cagacacacc gggcgctgca atcaatcgcg
     1621 aacgcagttt accgttgact tggatcggta tctccaactc ttcgcgttgt gcgagtccgg
     1681 gctcggggat cggccaccgc gggacttcgc ttaacaaccc tccatcgtgc cctaatcctt
     1741 cccacatctc ttcggcgatg tgcggtgcaa atggcgcgag catgatcacc agcgcttcca
     1801 atgcttcacg caccgcgaac acgtctgctt cggaagccgt cgccggatcg gcgttgaaat
     1861 cactcaactg attgaacagc tccatcaacg ccgccacact cgtgttcaaa tgcagttgct
     1921 caaagtcatt cgtgatcttc gcaattgtct ggtgtgtttt ccggcgcaag gtgcgcgcgc
     1981 cgtggctgaa atccaacgag ctcgactcac tgtcgcgtcg tgaaagtctc tcgtgccaac
     2041 gccacaccat cgtgtacacg cgccgcagaa aacgcaccgc gccctcgatg ccggactcag
     2101 accagcgcaa ctcgttctca gccggcgcgg caaacaagac aaacagccgc gtcgcatcgg
     2161 cgccaaacgc cgcgatcatc tcgtcaggat cgactccatt gccgagactc ttcgacatcg
     2221 ctttccattc atcggtgcct tcgacgcgat tggtcaccat tccctgcgtc aacaatcgct
     2281 tcaccggctc atcgaaatcg acgagtccga ggtcgcgcat gaacttggtc cagaagcgcg
     2341 tgtagatcag gtgcatgacg gcgtgggagt cgccgccgat gtactggtcc accggcgtcc
     2401 aacgtcgcgc tttgccggtg tcgaacggcg cgtcctcgtt ctgtgggtcg agatagcgga
     2461 agaagtacca cgacgaatcg acgaacgtgt ccatcgtgtc ggtctcgcgg cgcgccggag
     2521 actcacagtt cggacacgcc gtgttaacaa aatccggcac gccggccagc ggcgattcgc
     2581 ccgttccggt gaacgacgca gtctgcggca acacaacggg cagttgctcg taaggcaccg
     2641 gcaccatccc gcagttcgga cagtagataa caggcactgg cgcaccccag aatcgctgcc
     2701 gcgagatgcc ccaatcgcga atgcgataag tgaccgcggc ttcaccaaaa ccgtgcctct
     2761 tcgcgtattc cgccatctct ttgactgcga cttccgacag tttgccgccc cattcgccgc
     2821 tgtttatcag aacaccgtag ccggtgaacg actctcgcat ttcagccgag gcgtcgccgg
     2881 cggttgtcgc cactggttca gcccccacct gctcgtgagt gaccggtgca atcacctgcc
     2941 gaatcggcaa cgagtacttc tgcgcgaact caaaatcgcg ttcgtcgtga ccaggcacac
     3001 tcatcaccgc gcccgtgccg tattccatca gcacgtagtt cgcgacccac accggcaagt
     3061 tctctccact aaagggattg attgcgagca cccccgtgtt gagaccttcc ttcgcttcct
     3121 cgatttcatc gccgcgagtg gctcgctgtt gccggacttt ttccgcgaac gcctggatgt
     3181 cggccttcaa cgcagagcct tccagcaatt catcgagcaa cggatgttcg gctgaaagga
     3241 ccaccgcgtt ggcgccgtag atcgtatcga tgcgagtggt gaacacacga atcttcttgt
     3301 cgctatcctt gactggaaaa tcgacgtacg cgccttcgct gcggccgacc cagtcgctct
     3361 gccgtttcaa gactttttcg ggccaacccg cttcgatctg tttgatatcg gcaacgagtt
     3421 gctctgcgta gtcagtgatt cgcagaaacc actgctccaa gtcgcgcttc tcaacctgcg
     3481 tcccgcaacg ccagcaaatg ccgcctgacg actgttcatt cgacaaaaca gtctcttcct
     3541 tgggacacca gttgacctgc gacacctttt tataagcgag accgcgctcg agcattttca
     3601 gaaagaacca ctggtcccac ttgtagtaat ccggccggtg cgccgcgatc tcgcgcgacc
     3661 agtcataact gatgcccaga cgctgcaact gcccgcgcat atggctgatg ttctcttcag
     3721 tccagtcccg cgggttcact ccgcgtttga ttgccgcctg ttcggcgggc tggccgaaac
     3781 tgtcccaacc gatcggatgc agaacgttga aaccctgcag acgtttgaac cacgcgagcg
     3841 cgtcgccgat ggagtagttg cgtacgtgac ccatgtgtaa aaagcctgat ggatagggca
     3901 gcatttcgag cgcgtagaac ttctcgcgcg ggtcttcttc cttagcctgg aggacaccgc
     3961 tctccgccca ccgctgctgc cacttcttct cgatctgttc gggaaagtat ttttcgtcca
     4021 taaccataac cgtccaaatc tatcacagta atccgtgttt atctgtgtgc agatcggtta
     4081 tgaatgagcg cagcatggca agattcttga ggtcggcgcg cgggtcgtcg tagggagttt
     4141 caaggataaa cgcggcgtgc ttcagttttg ggtgctgggc tacgcggcgc agcgcatcgg
     4201 ctccaatgtg gccttcgccg atgtgccagt gacggtcgac acgggagttg taaaccgcgc
     4261 gcgagtcgtt gaaatgaata gcgcgaacgt tcttcacgcc gaccgtcttc tgcacgcgtt
     4321 gaatcaaagc ttcgagacca tccgcttcgc ggatgtcgta accggccgta aaagcgtgag
     4381 cagtatccat gcaaacgccg agcggaagct tcggacaagc gcgcatgatc gcatcgagat
     4441 gctcgaaccg gtggccgatg caatctcctt gtccggccgt gttctccagc aggatgcgta
     4501 gcgaaccgag ttgcaatccc ttgcacgcta tcttcaaact cgtcgcgcac aacgcaattc
     4561 cgtccgcctc gcacgcgccg cgcgagctgc ccggatggac cacgagataa tccacaccca
     4621 gcaacaacgc gcgctcaact tcctcgcgaa acgactcgcg cgatttttgc agcatcactt
     4681 catccgctgc cgcaaggttc accaggtagt tgcagtgaat caacacgggc gagatcttcg
     4741 taacgcgccg cgtcttgcgg aacaacaaca cgtcttcgag agtcagcggc ttggccatcc
     4801 aaccacgcgg attgcgtgag aacaactgca ccgtttcaca gccggtctcc ttcgcgacta
     4861 tcaacgcgtt gtgaacgccg cctttgatcg gggcgtgaaa acctattttc ggtctgcgtg
     4921 gcatccttgt cgtctgttct ttgttattga gcgcccatca cgcgcaacat attcccaccc
     4981 aggatcttct cgatatcgcc tgctgagtaa ccacgccgca acaactcttt cgtgagattc
     5041 ggcaagtcag ccacagtcgc gagatccgcc gccgtactta gaatcccatc aaaatccgaa
     5101 ccgataccaa catggtccac gccgaccagc ttcacgatgt gatcgatgtg gtccacgact
     5161 cgcgccacat aaaccggcgg cagtcgtcgc gcgtactccc tttgccgcac acgatcgcga
     5221 gccatcttct tccgcaccgc gccgcccggt tcgcttgcgg aagcgtcctg gaccatcggc
     5281 gcgatttcga ggtctgcttt tctcttcagc tcgctccaac cgggttcgat atgttccgga
     5341 taaaagatga cgttcacgac gccgcctgtt ttcgccaacg cgcgaatcat gtcgtcagtc
     5401 aggttgcgtg ggacgtttgc gagcgcgcgg caggctgagt gagtcgcgac cacgggtttt
     5461 gtggacgtgt tcacgacatc ccaaaacgtc ttgtccgaca cgtgcgaaac gtcaaccatc
     5521 attcccaacc ggttcatctc gcgcaccacg ctttttccaa agtcgttgag gccgcgtgtg
     5581 gctccggctt cgtctcccga ggaaccggcc caactcgtgc tcacgctcca ggcgggtgac
     5641 atgtagcgga cgccgagatc gtaatagcgc tgcacgtttt caagtttctc gtcgatcgcg
     5701 taaccgcctt cgagtcccat caacgccgcg atttttccgt cctggacggc gcggcggatg
     5761 tcatccgcgg aggtcgcgag ttgccatgtt tccgggtgtt tgtccgccag cgcacgaatc
     5821 gctgcgatct gatcgtctgc gcgtttcacc gcgcgctctc cgccgccgcc aaacaactgt
     5881 ggctcgaccc aaatcgagaa aaactgtgcg tctaaaccac ctgcgcgcgc acgaacggcg
     5941 tcgaagtggc cgtcgctgag ttgctgctgc aggtcgatcc gttcgtcgag caggcgttgt
     6001 acggtgtcaa cgtgcatatc gacggcgatt gcgcgtttat gaatagtgag tggatcgtca
     6061 gcagaatcct gggccacggt catcggttta ttgtcctggt tcatcgtggt tttgtggcag
     6121 ccggcgagaa acgctgcggt caaaatgagt gtaataaaaa agtgtggttc aagcgatcgc
     6181 attcgttgtt gcacgacgat cattacagga ggcaagcgtt cttgccaagc gaactacatc
     6241 tacggtattg tgccctattc ccacgcattt ttccttaagg agcaaaacac ttcatgttcg
     6301 caaaagatgg aaacccgact gatgtaaaaa cgcctcgcat ttgcgatgtt gtctggcata
     6361 acggtgaatt catcaagtgg gacgacgcgc gcgtgcatgt gatgtcacat ttgctgcact
     6421 atggttccag cgtttttgaa ggcatcaggt gttacgccac caaacgtggg ccagccgtct
     6481 tccgcctgcg cgagcacatg cagcggtttc tcaactcggc caaaatttat cgcatggacc
     6541 acccgtggac gctcgatgaa ctatgcgacg cggtggttga aattgcgcgg cgcagcgaga
     6601 tggatcagtg ctacatccgg ccgattctgt ttcgcagtct cgacgaagca catccggcgt
     6661 tcggtgtaaa tccgtttccc aacccgctcg cctgctacat tggcgcgtgg gactggggca
     6721 agtatctcgg cgacgaagca atcgagcagg gcgtggacgt ttgcgtgtcg acgtggaacc
     6781 ggttgacgcc aaactcaatg cccgcgatgg cgaagagcgg cgcaaattac atgaactcgc
     6841 agttgatcaa gatggaagcg cttttgaacg ggttcgctga aggcatcgcg ctcgacgatc
     6901 gtggttacgt gtcggaggga tctggggaaa atatctttct cgtcaccggc gacgttgtct
     6961 taacgccgcc actctcgtct tcaattctgc cgggcattac acgcgacagc gtgattcaga
     7021 tctgccacga actcggtatc acgatcaaag agcgcactat tcaacgcgcc gcgctctacg
     7081 tcgccgacga ggtctttttc tccgggaccg ccgcagaaat cacgccgata cgctcggtcg
     7141 accggatcac ggtcggcaga ggcgggcggg gtccaattac ggagcaggtt caaaaagttt
     7201 tcttcgaaat caccaaaggc gagcgcaaag cccccggcaa ctggctcacc tatgtaaacg
     7261 agaccgcgaa agcggaccgt aacggctttg cggccggctc tgtcgacgcc accgccgagt
     7321 tgaaggtttc cgtttagcgc cgcagccgtc ggtgtaacta tcaccatttg gctttctagc
     7381 tttctagcca cagattacgc ggattgtttt caaagaatct gtgtaatctg tggatagccc
     7441 cttccaattt aggtcatctt tccgcggtgg cgcggagtag tgtcctaatc tgaacgccga
     7501 aaggcgttcg ctaattccag cccagggttg cttcaaccct gggtcgaacg ttagcgtccc
     7561 gtcctaac
//
LOCUS       DASTEY010000526         7941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_324800, whole genome shotgun sequence.
ACCESSION   DASTEY010000526 DASTEY010000000
VERSION     DASTEY010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324800"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1983)
                     /locus_tag="VFO72_09230"
     CDS             complement(235..1983)
                     /locus_tag="VFO72_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9479516.1"
                     /translation="MRRVVGLSLSLLALISLSAAPIEAGSSSKTLATISGLVTDNKGN
                     PLSGALVSLLREGATKQTVKHTLTNREGKFVAKVVPGRYGIKAIATGFSEVAFNAVDV
                     KAAQELVYKFNLEPVGYGNTLPERRRDRDDVKWTLRSKQTSRSIFQVREGEDATIQAV
                     TDAENSTTDMTATPEPEWARTGYQPRTHGVIESYFGSNGFASSYIGFNFATSTPISDQ
                     VELIFSGQTGTEYAPQRLEATTRFRVGDRHKVGVTAAGARYEAPVWTGSVNEGGDLAG
                     QVSLRAVDEWIVRDGIVIVMGLDYSRFIGAGGAQSFSPRFGVQFDANARTRVKAAFAS
                     GGDEEGIQSVATFENEQVVFRRQALRPVAFVDGQAVVERSHRLEFGVERVLDNSSNVE
                     ATAFFDTTSGRGVGLLSSPATAFSGHAGDTFASVANQQGSSRGVRVVYTRRLNRVWTA
                     SAAYAFGRGQRLSGAGFTSPAELFENGLFQTAALQLGAGFRSGTQVRTVFRFSPEATV
                     FAIDPFAGRLGVYDPSLSIQVTQDLPSFGLPVRAEAILDARNLLDAQIASDNGEFITF
                     VGNGRRSVRGGISVRF"
     gene            2345..3625
                     /locus_tag="VFO72_09235"
     CDS             2345..3625
                     /locus_tag="VFO72_09235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9479517.1"
                     /translation="MIDDVHTTRLPNGIAVLTEHMPGLRSVTAGIWVRRGSRHESADL
                     NGICHFIEHAVFKGTRHRSAHDIAVETDRLGGHLDAYTTHEMTGFTIKVADTALSQAM
                     DLLADLISNPRFEQEDLEREQRVILEEMKMVEDTPDELLGELFNAAYFPNQPLGRPIE
                     GTRETVPTFDHKTTVAFHAREFSYSNLVVAAAGNVDHNRLLELVGRAFNGCESGAAVR
                     PRPLDGSRPRPAAPILIEQKNELEQAHLVLATPWPSALSEDRYSASLLASIIGGGTSS
                     RLWQKIREERGLVYSVGAAGSAFSDVGVFNVYAGTSPQQLDEVLELSLKELRQAVRNA
                     VTEEELRIAKDQAISSILLGLESSSARASTLARQEIIHGRRISPDEVIEKLRHTTIEE
                     LQEVARTYFKSETIALGALGNLNGFKVDRSRLSI"
     gene            3640..4464
                     /locus_tag="VFO72_09240"
     CDS             3640..4464
                     /locus_tag="VFO72_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9479518.1"
                     /translation="MKLTVLGSGSTGNAVLIVAGNTRVLVDAGLSAKEIVRRLALMGE
                     DAQQLDGVLVTHEHGDHAGGLRVLLGSVDCPVYISAKTLDAYVSERFNVNTDEPRRRA
                     HALNGRVEEIESSRDFRIGEIDFHPFTIPHDAVDNFGFTATHHGVKIATVMDFGHITT
                     LVAEQLRGCAAIVIESNHSRDMLKACDSYPWELKQRILSRLGHLSNEDLADWIKEGFD
                     GTAKYIVLAHLSQRANNPYLAKITAEVALQEKAPLFVGDTEVCLSYPKEPTPWIEV"
     gene            4599..4994
                     /locus_tag="VFO72_09245"
     CDS             4599..4994
                     /locus_tag="VFO72_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HET9479519.1"
                     /translation="MFACACGLRAERGRVPSEVQEVVTTVGDQIAQERYEQIYNDSSD
                     LWKQDVTLEQSNQVFKTLRDKLGKVESRSLHSATEQQNSGGALKGHVYILTYQTRFQR
                     GDGMETFTLVEQNGRWQLARYFVNSTALK"
     gene            5020..6315
                     /gene="purB"
                     /locus_tag="VFO72_09250"
     CDS             5020..6315
                     /gene="purB"
                     /locus_tag="VFO72_09250"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9479520.1"
                     /translation="MIDRYTLPEMGALWSEQNKFQKWLDVEIAVCEVHAENGTIPREA
                     LEQIKARAKFSVERINEIEKTTNHDVIAFTTNLAESIGDASRFVHFGLTSSDVVDTAN
                     ALLLRDASGILLKKVDALIDVLKRRALEFKNTPQVGRTHGIHAEPTSFGLTFALWFDE
                     MRRNRERLLRAREAVAVGKISGAVGAFAHLDPSVEESVCARLGLRAAPVSTQVIQRDI
                     YAEYLNTLAVIASSLEKFALNVRHWQRTEVREAQERFTKGQKGSSAMPHKRNPIISER
                     ICGMARVVRANSLVGLENVALWHERDISHSSAERVVLPDSSIALDYMLQKAIGLIEGL
                     VVYPDRMLENLGATRGLIFSGQLLLALTDKGVSREAAYEWVQRNAMRVWDEDLEFHAL
                     VKTDPDITSRLSAAEIDRTFSLEHYLRNVDQIFKRLGIT"
     gene            complement(6308..6718)
                     /locus_tag="VFO72_09255"
     CDS             complement(6308..6718)
                     /locus_tag="VFO72_09255"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET9479521.1"
                     /translation="MQNPPPVQTTKSSTGLDENIAALLAIIFGWLGGLIFFLIEKESR
                     FVRFYAMQSILLNVLVLVVFIVLWIATVVLVIIGAALGDAVGTLVGILSTLLWLALGV
                     GVLIAVVMCLIKAYQKQYFKLPIIGNMAEKFSAK"
     gene            6813..7058
                     /gene="purS"
                     /locus_tag="VFO72_09260"
     CDS             6813..7058
                     /gene="purS"
                     /locus_tag="VFO72_09260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET9479522.1"
                     /translation="MKAKVYVNLKAGVLDPQGKAIQHSVELLGFSGIADVRQGKYFEI
                     ALDDSVDTQHARESVEKMAREVLSNPIIEDYRVEIEA"
     gene            7055..7753
                     /gene="purQ"
                     /locus_tag="VFO72_09265"
     CDS             7055..7753
                     /gene="purQ"
                     /locus_tag="VFO72_09265"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET9479523.1"
                     /translation="MKFGVIVFPGSNCDHDAYHVISKHVGQPVDFIWHRETDLSTYDA
                     VIIPGGFSYGDYLRAGALARFSPVMNSVKKFAESGRFVLGICNGFQILCEAGLLPGAL
                     IRNRDLHFVCEHVYVRVETSDSPFTNELKRGAVLRIPIAHAEGNYVCDDQTLAELQRE
                     DRIVFRYCEKDGSITDAANPNGSRDNIAGICNSERNVLGLMPHPERACEDLLGSSDGR
                     DIFRSLAATLAAAV"
BASE COUNT         1809 a         2233 c         2219 g         1680 t
ORIGIN      
        1 tgaggacgcc ggcagtgatc aagagcgccg ctccgatcag caaataccag gctttagttg
       61 tgagtattga tcctttcaat gcttcctcaa cttcgcctcc gaatttctta accaggcgat
      121 ctgaatagtt taggcaagga atgttccgag taatccgcgc ggtttactgg cggttctgct
      181 ggcctaatcg ttgaggttaa tccaactttt cggagttttc cgagaatttg gaagctaaaa
      241 cctgactgaa attccaccgc gaacagaccg ccggccatta ccaacgaagg ttatgaactc
      301 accgttgtcg gacgcgatct gcgcatccag gagattccgc gcgtcgagaa tggcttcggc
      361 ccgaacgggc aagccaaagc tcggcaaatc ctgtgttacc tggatgctca gagaaggatc
      421 gtaaacaccc agtcttccgg caaatggatc gatcgcgaag accgtcgctt ccggagagaa
      481 gcggaagacg gttcgcacct gcgtaccact acgaaaacct gctcccagtt gcaaggctgc
      541 ggtttgaaat agaccgttct cgaagagctc ggcgggactc gtaaaacctg cgcctgagag
      601 tcgttgacca cgtccaaacg cgtaagccgc cgaagccgtc cacacacgat tcagacgacg
      661 cgtgtagacc acgcggacgc cgcgcgacga tccctgctga ttcgccacac tcgcgaacgt
      721 gtcaccggcg tgacctgaaa atgcagttgc cggagaactc agcaagccga cgccgcggcc
      781 actcgtcgtg tcgaagaacg cagtcgcttc gacgttcgag ctgttgtcga gcactcgctc
      841 cactccgaat tcaagccgat ggctgcgttc taccacggcc tgaccatcga caaaagcaac
      901 cggccgcaaa gcctgacgtc tgaagacaac ttgttcgttt tcgaaagtcg cgacactttg
      961 aatgccctct tcatcgccgc cggaagcgaa cgccgctttc acgcgcgtgc gcgcgtttgc
     1021 gtcaaactga actccgaatc gaggactaaa actctgcgcg ccgcccgcac cgatgaaacg
     1081 cgagtagtcg agtcccatca cgatcacgat gccatcgcgc acgatccact catccaccgc
     1141 tcgcaacgaa acctgaccgg cgaggtcgcc accttcatta acgctgccgg tccaaaccgg
     1201 cgcttcgtaa cgcgcacccg ccgcagtcac accgactttg tgccggtcgc cgacgcgaaa
     1261 acgcgtcgta gcttccagtc gctgcggagc gtactccgtt cctgtttgtc cggaaaagat
     1321 gagttcgact tgatctgaaa tgggtgtaga ggtggcgaag ttgaaaccaa tgtacgaaga
     1381 agcgaacccg ttactgccaa aatagctctc tatgacgccg tgcgttcttg gctggtaacc
     1441 cgtgcgggcc cattcaggtt ccggtgtggc tgtcatatca gtggtgctgt tttcagcgtc
     1501 ggtcactgct tgaatcgtgg cgtcttcacc ttcccgcacc tggaagatcg aacggcttgt
     1561 ttgctttgat cgcagcgtcc atttcacgtc gtcgcgatcg cggcgccgct caggcagcgt
     1621 gttgccgtaa ccaaccggtt caaggttgaa cttgtaaacc agttcctgcg ccgccttcac
     1681 gtcgacggcg ttgaaggcaa cctcactaaa tccggttgcg atagctttaa tcccataacg
     1741 gccggggact accttcgcga caaacttgcc ctctcgattt gtaagcgtgt gtttgactgt
     1801 ttgtttggtg gcgccttcgc gcagcaaaga cacgagtgcc ccggagagcg gatttccctt
     1861 gttgtcggtg acgaggcccg agattgtcgc gagtgtctta cttgaactac ctgcctcaat
     1921 aggggcagca gaaagcgaaa ttagtgcgag cagggataag cttagaccga caactcttct
     1981 catgcgttaa cgacctccgc tacgaaagtc ccatcgggcc gccgtcttcg ggagtgaaca
     2041 ggccgaactg gttcaagcga ggagacttac cagaaccagc ccaataaggg gaacattgtc
     2101 gttcaggccc ccaaaagtgt caagaacggg gcggtaaata ctggaaacgc aggcgccccg
     2161 cctgcggggt gcgtatcggg cgctgtccgt gagttgcagg cgagacgcct gcgttcccag
     2221 aaacgtaaat caggtcattt gcggctccgt tttggagcgt gctaggattc agaccccctg
     2281 aacagccgca gtgcacgtta tgatccttgc ggttttcgtt tccagctgat ttggttcccg
     2341 gcgaatgatt gacgacgttc acacgacaag actcccgaat ggcattgccg tactgactga
     2401 gcatatgccc gggctacggt cggtgaccgc cggaatctgg gtacgccgcg gatcgcgaca
     2461 cgaatcggct gatctcaacg gcatctgcca ctttatcgaa cacgcggttt tcaaagggac
     2521 gcggcatcgt agtgcgcacg atattgccgt ggaaaccgat cgtctgggcg gacacctcga
     2581 cgcctacacg acccatgaga tgacaggctt caccataaag gttgcggata ccgcgctttc
     2641 gcaagccatg gatctgctcg cagatctcat ttccaacccg cgttttgaac aggaagatct
     2701 cgagcgtgag caaagagtca ttctcgaaga gatgaagatg gtcgaggaca cgcccgacga
     2761 actgctcggc gagctcttca acgccgcgta cttcccgaat caaccactcg gacgtcccat
     2821 cgaaggtact cgcgaaacag ttcccacctt cgatcacaaa acgaccgtcg catttcatgc
     2881 gcgtgagttc tcgtacagca acctggtggt cgccgccgcg ggaaatgtcg atcacaatcg
     2941 tctgctcgaa ctggtgggca gggcgttcaa tggttgcgaa agtggcgccg ccgtgcgacc
     3001 tcgcccgtta gatggcagtc ggcctcgtcc ggccgcgccg atcttgatcg agcagaagaa
     3061 tgaactcgag caagcgcatc tcgtgctcgc gacaccatgg ccgtctgcgt tgagtgaaga
     3121 tcgttactct gcaagtttgc tggcgtcgat catcggcggt ggcacatcga gccggctgtg
     3181 gcagaagatt cgcgaggagc gtggcctggt ttattcagtc ggcgccgcgg gcagtgcttt
     3241 ctccgatgtc ggagtgttca atgtttacgc cggaacttca ccgcagcaac tcgatgaggt
     3301 gctggagctt tcgttgaagg aactgcgcca ggccgtgcgc aacgctgtga cggaagaaga
     3361 gctgcgcatc gcaaaggacc aggccatctc gtcgatcctg cttggactcg aatcgtcaag
     3421 tgcacgcgcc agcacgctcg cgcgccagga gatcattcat ggccggcgca tctcacccga
     3481 tgaagtgatc gaaaaactca gacacaccac gatcgaagag cttcaggaag ttgcgcgcac
     3541 ctacttcaaa tcggaaacaa tcgcgctcgg cgcgctcgga aatctcaacg gctttaaagt
     3601 cgatcgctcc cgcttatcaa tctgatatag tgccgcacaa tgaaacttac cgttctcggt
     3661 agcggatcaa ctggtaacgc agttttgatc gttgccggca atacgcgcgt cctggttgat
     3721 gctggtctga gcgctaaaga gatcgttcgc cggctggcgc tcatgggtga ggacgcgcaa
     3781 caactcgatg gcgtgctcgt aacgcacgaa catggcgatc atgccggtgg gctgcgcgtg
     3841 ctgctcggtt cggttgattg tcccgtctac atttccgcga aaacactcga cgcttacgtc
     3901 agcgaacggt tcaacgtcaa caccgatgaa ccgcgccggc gtgctcatgc gctgaacggt
     3961 cgcgtcgaag agatcgaatc gagcagagac ttccgcatcg gcgagatcga ttttcacccg
     4021 ttcacgattc cacacgatgc cgtggacaat ttcggtttta ccgcaacaca tcacggcgtg
     4081 aagatcgcga ccgtgatgga cttcggccac atcacgacgc tggttgcgga acaactgcgt
     4141 ggttgcgcgg cgatcgtgat cgaatcaaac cacagtcgcg atatgctcaa agcctgtgac
     4201 tcttatccgt gggaactgaa acagcgcatt ctgtcgcggc tcgggcacct gtcgaacgaa
     4261 gacttagcgg actggattaa agaaggattt gacggcactg cgaagtacat cgtgctcgca
     4321 cacctttcac aacgggcaaa caatccgtac ctcgcgaaga tcaccgctga ggtcgcgttg
     4381 caggagaagg cgccgctgtt tgttggcgat acggaagttt gtctgtccta tccaaaggag
     4441 ccgacgccct ggatcgaagt gtaaacgctg gaactaaagg aacgcttatc cctgggagcg
     4501 caggcgactc gcctgcacgg cagcagcgca tacggcgcct acccaaaccg gtagccgaga
     4561 cgccttcggt cccagggata gccgttcttt tgtttgtgat gttcgcctgt gcgtgcggtc
     4621 tgcgcgccga acgcggccga gttcccagtg aagtccagga ggtcgtcacg actgtcggag
     4681 atcaaatagc ccaggaacgt tacgagcaga tttataacga ctcgtcggat ctctggaaac
     4741 aggacgttac gctcgaacag tcaaaccagg ttttcaaaac cctgcgcgat aagctcggca
     4801 aagttgagag ccgcagtctt cattcggcaa ctgaacagca aaactccggt ggcgcattga
     4861 agggtcatgt ttacatactg acgtatcaaa cacgattcca acgcggcgac ggaatggaga
     4921 cttttacgct tgtcgaacag aacggccgct ggcaactggc gcgctatttt gtaaattcga
     4981 cggcgttgaa ataagccacg atatacacag agcacacaga tgattgatcg ttacacgttg
     5041 cccgagatgg gcgcgctctg gtcagagcag aacaagtttc agaaatggtt ggacgtggag
     5101 atagcggtgt gtgaagttca cgccgagaac ggaacgatcc cgcgtgaggc gctggagcag
     5161 atcaaggccc gcgcaaagtt ttccgtcgaa cgaatcaacg aaatagagaa gacgactaac
     5221 cacgacgtca tcgccttcac cacaaacctc gcggaatcga tcggcgacgc gagtcgcttc
     5281 gttcatttcg gcctcacttc gtcagacgtt gtcgacactg ctaacgcact actgctgcgc
     5341 gatgcgtccg gcatactgct gaaaaaggta gacgcgctga tcgacgtgct gaaacgtcgc
     5401 gcgctcgaat tcaaaaacac gccgcaggtg ggccgaacac acggcattca tgcggaaccg
     5461 acgtctttcg gtttgacgtt tgctctctgg ttcgacgaaa tgcgccgcaa tcgcgagcgg
     5521 ctactgcgcg cgcgcgaagc tgtggcagta ggaaagatca gcggcgcagt cggcgcgttt
     5581 gcgcatctgg atccgtcagt cgaagagagc gtttgtgctc gattgggttt gagagctgcg
     5641 ccggtgtcga cgcaggtcat ccagcgcgac atttatgcag agtacctgaa cacactggcc
     5701 gtgatcgctt cgtcgttaga aaagtttgcg ttgaacgtcc gtcactggca gcgcactgaa
     5761 gtgcgagagg cgcaggaacg tttcaccaaa ggccagaaag gttcgagcgc aatgccacat
     5821 aagcgaaatc cgatcatatc ggagcgcatt tgtggcatgg cgcgcgtcgt tcgcgccaac
     5881 agtctggtcg gactcgagaa cgtcgcgctg tggcacgagc gcgatatttc acactcgtct
     5941 gcggaacgcg tcgtgttgcc tgattcgagc atcgcgctcg actacatgct gcaaaaagcg
     6001 attgggttga ttgagggtct cgtggtctat cccgaccgga tgctggaaaa cctcggcgcg
     6061 acacgcgggt tgatcttcag cggccaactg ttgctggcgc tcaccgacaa aggcgtgtca
     6121 cgcgaggcgg cgtacgagtg ggtgcaacgc aatgcgatgc gtgtttggga cgaggatctt
     6181 gagtttcacg cgctggtgaa aacagatcct gatattactt ctcgactgtc cgccgcagag
     6241 atcgatcgca cgttctcgct cgaacactac ctgcgcaacg tggatcagat cttcaaacga
     6301 ttggggatta cttagctgag aacttttcag ccatattgcc gatgatcggc agtttgaaat
     6361 actgcttctg atacgctttg atcaggcaca tcactacggc aatcagtacg cctacgccca
     6421 aagccaacca aaggagagtg ctgagtatac cgaccagagt gccgacggcg tcgccaagcg
     6481 ccgcgccgat tatcacgagc actacggtcg cgatccataa cacgatgaat actacaagaa
     6541 ccaagacgtt gagcaggatc gactgcatcg cataaaaccg tacaaagcga ctttccttct
     6601 caatcaggaa gaaaatcagt cctccgagcc aaccaaaaat aatggcgagc agggcggcaa
     6661 tgttctcatc gagtccggta ctagacttcg tcgtttggac gggcggcggg ttttgcatta
     6721 taagtttctc ctgttggggc gttttgagcg gaggatatta gttcattaac tgcttcttaa
     6781 gcaacgtttt attcatttaa gacaggtcgc acatgaaagc caaagtttat gtaaacctga
     6841 aggccggagt tctcgacccg cagggcaaag ccatccagca ttccgtcgag ctgctgggat
     6901 tttccgggat cgcggacgtg cgccagggca agtacttcga aatcgcgctc gacgactcgg
     6961 tcgataccca acacgcccgc gagtcggtcg agaaaatggc gcgcgaagtg ttgtcaaacc
     7021 cgataatcga agactaccgc gtggagatag aagcgtgaag tttggagtaa tagtttttcc
     7081 cggttcgaat tgcgaccacg atgcctacca cgtgatttca aaacacgtcg gtcagccggt
     7141 ggactttatc tggcatcggg agacggattt gagcacctac gacgccgtga tcatccccgg
     7201 cggcttctct tatggcgact acctgcgggc cggagcgctc gcccggtttt caccggtgat
     7261 gaactcggtc aagaagtttg ccgaaagcgg ccgcttcgtg ctgggaatct gtaacggttt
     7321 tcagatcctg tgtgaagcgg gacttttgcc gggtgcgctg atccgcaacc gtgacctgca
     7381 tttcgtttgc gagcacgttt acgtgcgcgt cgaaacttcc gacagtccgt tcacaaacga
     7441 actcaaacgt ggtgcggtgc tgcgtattcc aattgcccat gccgaaggca actacgtctg
     7501 cgacgatcaa acgctggctg aattacaacg tgaggatcgc atcgtctttc gatattgcga
     7561 aaaggacggc tccatcacag acgccgcgaa cccgaatggg tcgcgcgaca acatcgcggg
     7621 catttgtaac agcgagcgca atgtgctcgg actgatgccg caccctgaac gcgcttgcga
     7681 agatctgctc ggctccagcg acggacgcga tatctttcgc tcgcttgcgg cgactctcgc
     7741 tgccgctgtt taagaacact cagaacatga aacgtacact gccattcatc ataattctcg
     7801 cggtgctcgc cattgccctt ggttccgcat ggtatttgac tcgtcctacg cctggaccaa
     7861 gcaccgcaac tccagcgtca tccgggcaac gttcatcaaa cgcaccggca cagccggccg
     7921 cgctaagagg tattccgggc g
//
LOCUS       DASTEY010000527         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1231691, whole genome shotgun sequence.
ACCESSION   DASTEY010000527 DASTEY010000000
VERSION     DASTEY010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231691"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            597..2711
                     /locus_tag="VFO72_09270"
     CDS             597..2711
                     /locus_tag="VFO72_09270"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HET9479524.1"
                     /translation="MRLRRFLTALLLALLSISTLHVTALAEEGMWPFNNVPRAEIKKK
                     YGFEVTDEWLNKVRLASVRFNNGGSGSFVSSDGLVLTNYHIVEDIVGEVSTPQKDYAK
                     EGFVAKTRAEEIKAPSLELNVLMSIEDVTSRVNGAVKAGMSDADAFAARRAEISAIEA
                     ESTKTTGLRSDVVTLYQGGQYNLYRYKKYTDVRLVFVPEFQAAFFGGDPDNFNFPRFN
                     IDMALVRVYENDKPVRPEHYFKWSTAGAKEGTLVFVTGHPGTTSRLSTVAHLEALRDT
                     AIPIVIRLLERRETMLKKYMALGEEQTRQAQSELNSVQNSLKVYRGQLAGLKDPALMA
                     RKKREEDALRKSIAANPERQKMYGDAWDSIAKAYQTYPSYIRERRIFDQAGGFNSTLF
                     SFARALVRLAAENEKPNAERLLEYTDARRASLELALYSPAPIHEEFEKLKLTDSLGFM
                     VELLGADNPLVKQVLDGKTPEARANELISGTKLKDPAYRKEVAAGGVKAIESSTDPMI
                     VVAREIDAKSREVRKRYESEVVGVERANYAKIARALFETEGTKLYPDATFTLRLSYGT
                     VSGYMENGKKVPPFTTLGGLFARSDSFKRQFPYNLPQRWYDKKSSIDLNTPFNFVSTN
                     DIIGGNSGSPTINQNGELVGLIFDGNIQSLVGDFMYDGSVNRAISVDSRGMLEVLKKV
                     FNANEIVAELVGQAATTKAAGR"
     gene            complement(2873..>3470)
                     /locus_tag="VFO72_09275"
     CDS             complement(2873..>3470)
                     /locus_tag="VFO72_09275"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9479525.1"
                     /translation="GLEDGLFPHSRSATDPAELEEERRLAYVAMTRAERFLYVTHAMK
                     RRVYGEELASEPSQFLNEMPIDLLEDLSLGKSWLSFARGSSAIDYEHGEYRKEKKRYM
                     GKTYDSVDSIAEFFKQRSRQLGERPTKSKSQTPTPPKAPASASSEIVPGSYVKHSKYG
                     RGLVLRREGAGESTKLTVSFPGYGQKKLVEKYAGLEKA"
BASE COUNT          779 a          939 c          968 g          784 t
ORIGIN      
        1 ggtgcaggcg agacgcctgc gctcccaggg gttgatagca acgcttttgt ctgcgtgcca
       61 gtcaaaagtt tcgcgacgaa gccaacacat tttgatgaaa ctaacgcata gtttgtcttt
      121 taaaagactc aagctttctt atacataccg tgcgaatcca cgaatccttt gggggtattc
      181 tcgatgaggg tagaaaagca gattagattc aacgagtggc tgctgggctc aacgattctg
      241 tgtcagtcgt gcggttgtct ttggctggtt ccagcggcgc agaacgaaga tcgctacact
      301 tgcaggcagt gtggcagcga agtgacaagc aaaccggctg ttcgcggcga tcagaatact
      361 aagacctcaa acacgcgctg agctttgtac tttgagcttt gttctttgta cgtgggttct
      421 caccgaccac caacaaagaa caaaaaccca agctcaaagc tctaccaccc acatttgact
      481 attaaccctc ccggggttta cgtttttctg cgtctgcgtg ccaattgggg catctaattc
      541 gacggttcaa cgctctaaaa ctgtctcctg caatatctac tctcgaggat cactagatga
      601 gattaagaag attcctgacg gcgctgttat tggcgctctt aagtatttca acgctccatg
      661 ttaccgccct cgcggaagag ggaatgtggc cgttcaataa cgtcccgcga gccgaaatca
      721 agaaaaagta cgggtttgag gttaccgacg aatggttgaa caaagttcgt ctcgcttccg
      781 tcaggttcaa caacggcggg tccggttctt tcgtctcttc tgacgggttg gtgttgacca
      841 actatcacat cgtcgaagac atcgtgggcg aagtcagcac gccgcaaaag gattacgcga
      901 aggaaggttt tgtcgccaag acccgagctg aggagattaa agcgccgagc ctcgagttga
      961 atgttttgat gagcatcgaa gacgtcactt cacgcgtaaa cggtgcagtc aaagcaggca
     1021 tgagcgacgc cgatgcgttc gccgcccggc gggctgagat ctctgcgatc gaagctgagt
     1081 cgaccaagac gaccggcctg cgatccgacg tggtcacgct ctatcagggt ggtcagtaca
     1141 atctctatcg ctacaagaag tacaccgacg tccgtctggt gttcgtgccg gagtttcagg
     1201 cggcgttctt cggcggcgat ccggacaact tcaatttccc gcgcttcaac atcgacatgg
     1261 cgctggtgcg cgtttacgaa aacgataaac cggtgcgtcc tgaacattac ttcaagtggt
     1321 ccaccgccgg cgccaaagag gggactctgg ttttcgtgac cggtcatccg ggcactacgt
     1381 cgcgcttgag cacggttgcc catctggagg cgcttcgcga tacagctatt ccaatcgtca
     1441 ttcggctgct cgaacggcgg gaaaccatgc tcaagaagta catggcgttg ggcgaagagc
     1501 agactcgtca ggcacagagc gagttgaata gcgtgcagaa cagtctcaag gtttatcgcg
     1561 gtcagcttgc cggactaaaa gatccagcgc tgatggcgcg caaaaagaga gaagaagacg
     1621 ccctgcgcaa gtcgatcgct gctaaccctg aacgccagaa gatgtatggc gatgcatggg
     1681 actcgattgc caaagcttat cagacgtatc cgagctacat tcgtgagcgt cgcatcttcg
     1741 atcaggctgg tggttttaac agcacgctct tcagtttcgc gcgcgcgctg gttcgccttg
     1801 cggctgaaaa tgagaaacca aacgccgagc ggttgctgga atacactgac gcacgtcgcg
     1861 cgtcgttgga gctcgcgctc tattctccag ctccgattca cgaagagttc gagaagctga
     1921 agctcaccga ctcgctcggt ttcatggttg aactgctcgg cgccgacaac ccgctcgtga
     1981 agcaagtgct tgatggtaag acgcccgaag cacgagcgaa cgagttgatc agtggcacca
     2041 aactgaaaga cccggcttac cgcaaagaag tggcggccgg cggcgtcaag gcgattgaga
     2101 gttcgaccga tccgatgatc gtcgtcgcgc gtgagatcga tgcgaagtcg cgtgaagtgc
     2161 gcaagcgtta tgaaagcgaa gtggtaggtg tcgagcgtgc gaactacgct aagatcgcgc
     2221 gtgcgttgtt cgagactgaa ggcacgaagc tttatccgga tgcgaccttc acgctgcgcc
     2281 tgtcgtacgg tactgtctcg ggctacatgg agaacggcaa gaaggtccca ccgttcacga
     2341 cgctcggcgg actgttcgcg cgttccgatt cgttcaagcg acagttccca tacaatttgc
     2401 cgcagcgctg gtacgacaag aagtcgtcga tcgatctgaa cacgccgttc aactttgttt
     2461 cgacgaacga catcatcggc ggcaactccg gttcacccac gattaaccag aacggtgaac
     2521 tcgtcgggtt gatcttcgac ggcaacattc agtcgctggt cggcgacttc atgtacgacg
     2581 gatcggttaa tcgtgcgatt tcggtagatt cgcgggggat gttggaggta ctgaagaaag
     2641 tcttcaacgc gaacgagatc gtggccgagc tggtcggtca agcggccaca acgaaggcgg
     2701 ctggacgcta agctttctgc cggccctttg ggcctcgacc catgctgttt gtgatccagg
     2761 ggctgcgcgc ttcgcgcttg cccctggcta tcttcttgcc gcgccttcgg cgctccagca
     2821 ggaaagctcg cgtaacgata tcgcttcgag gctcaggctt aacgcgtcga cgctaagctt
     2881 tctcgaggcc ggcgtatttc tcgacgagct tcttctgtcc gtaaccggga aaactcactg
     2941 tcaacttggt cgattctccg gcgccctcac gccgcagaac cagaccacgt ccatacttcg
     3001 aatgcttcac gtaagatccc ggtacgatct cgctcgatgc tgaagccggt gcttttggcg
     3061 gcgttggggt ttgtgattta gatttcgttg gtctctcacc caactgccgg ctgcgctgct
     3121 taaaaaattc agcgatggaa tcgacgctgt cgtaagtctt gcccatgtat ctcttcttct
     3181 ctttccgata ctcaccgtgc tcgtaatcga ttgccgacga tccgcgcgca aacgacaacc
     3241 acgacttgcc gagactgaga tcttccagca gatcgatagg catctcgttg agaaactgcg
     3301 acggttcaga agcgagttct tcaccgtaaa cgcgccgctt catcgcatgg gtcacgtaaa
     3361 gaaaacgctc ggcgcgcgtc atcgcaacgt aagcaagccg gcgttcttct tcgagctctg
     3421 ccggatcggt ggccgagcgc gaatgcggaa acagtccgtc ttcgagaccg
//
LOCUS       DASTEY010000528         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_786384, whole genome shotgun sequence.
ACCESSION   DASTEY010000528 DASTEY010000000
VERSION     DASTEY010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786384"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFO72_09280"
     CDS             complement(<1..433)
                     /locus_tag="VFO72_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479526.1"
                     /translation="MQTVQNSNRGLSGWCERAIIGCIFLIAIFAPHSIAATQTFWLLG
                     LFFWVLRFAFYPRPLLFRTPIDYLLLGFFVLSGLSGVFSYSPIVSIGKMRAASLFTVI
                     YLVAQNVRSVRLAHVLALTLIGSCMLDVVWTAGQLALGRGVK"
     gene            478..1710
                     /locus_tag="VFO72_09285"
     CDS             478..1710
                     /locus_tag="VFO72_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9479527.1"
                     /translation="MSRIIVALVFCALASPVWSQSASPADEVKAIQEDPRVIAAFEYI
                     DKDRDAILREWIAITEINAPSKHEQERAKFLESLLRKHKLEIRYDSAGNLIATRKGTV
                     GNPVAVFDAHMDTVFQPGLKIKATVRDGRVYAPGVGDDTRNIEALLATIRALDAAQIK
                     TKGDLVFLFTTDEEAGMTGAKQYTKDNKGKVDYYVALDGGYEGFTYAGIGINWYRHHF
                     IGPGGHTRSRTPPYSASLPLARAISRIYELKVPTDPSSNLNIGMLGGSEVVNAKAADA
                     WFTVDLRSTSNEVIADLEKQIAAILDEEAKRAGMTLKTDLISSSPAAVLPGNRESHLV
                     KIAEAVHRAMGFNPPIGNAGSNNSSAALLQGISSISTGAGPCSDAHALTENCEIEPLY
                     KGIKKVLLLAVALAGLTQ"
     gene            complement(1704..>2187)
                     /locus_tag="VFO72_09290"
     CDS             complement(1704..>2187)
                     /locus_tag="VFO72_09290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9479528.1"
                     /translation="DGRLFLLYKFRTMIADADPELHREYQRAFIAGRAEANVGNAQKP
                     TYKLFADPRITRVGKVLRRPSLDEVPQLLNVLMGDMSVVGPRPPIPYEVEAYELWHRK
                     RLDMKPGLTGLWQVSGRNRLPFEEMVRLDLFYIENWSLLLDLKIILRTGFVMIGREGH
                     "
BASE COUNT          549 a          605 c          590 g          443 t
ORIGIN      
        1 ctttcacgcc gcgtcccaac gccaactgac ccgcagtcca aacgacgtcc agcatgcacg
       61 agccgatcaa cgtcaatgcg agcacgtggg ccagtcgcac tgaacgaacg ttttgcgcga
      121 cgaggtaaat gaccgtgaaa agacttgccg cgcgcatctt tccgatcgac acgatcggac
      181 tgtaggaaaa cacgcccgat aagccactca acacgaaaaa cccgagcaac aaataatcaa
      241 tgggtgtgcg gaaaagcaga ggtcgcgggt agaaggcaaa ccgcaacacc cagaagaaca
      301 gtcccaacag ccaaaaggtc tgtgtcgcgg cgattgagtg cggagcaaaa attgcaatga
      361 ggaagatgca gccgataatg gcacgttcgc accatccaga taatccgcga ttcgaattct
      421 gcaccgtctg catttagaat gttcgcggtc tactatattc cattcaaagg taattgcatg
      481 agccgaatta ttgttgcatt ggtgttttgt gcgctggcaa gtccggtttg gtcccagtct
      541 gcctcgcctg cggatgaagt aaaggcgatt caggaagacc cgcgcgtcat agcggccttc
      601 gaatacatcg acaaggatcg cgacgcaatc ctgcgcgagt ggattgccat caccgagatc
      661 aacgcaccgt cgaaacacga acaggaacgt gccaagttcc ttgaaagctt gttgcgaaaa
      721 cacaaactcg agattcgtta cgactccgcc ggtaatctaa tcgcgacgcg caaaggaacc
      781 gtcggcaatc cggtcgctgt attcgacgca cacatggaca ccgtctttca accgggcttg
      841 aagatcaaag ccactgttcg cgacgggcgc gtctatgctc ccggcgttgg cgacgacaca
      901 cgcaacatcg aagcgctgct cgccacgatt cgcgcgctcg atgcggcaca gatcaagact
      961 aaaggcgacc tcgtctttct tttcaccact gacgaagaag ccggcatgac gggcgccaag
     1021 cagtacacca aagataacaa gggcaaggtt gattattacg ttgctctcga tggcggctat
     1081 gaaggtttca cttatgcggg catcggcatc aactggtatc ggcaccactt cattgggcca
     1141 ggcgggcaca cacgttcgcg cacgccgccg tactcagcgt cgttgccgct ggcgcgcgca
     1201 atttcgcgca tttacgagct gaaggttccg accgatccgt cgtcaaactt gaacatcggc
     1261 atgttaggcg gatcggaagt cgtgaacgca aaagcggccg atgcgtggtt tacagttgac
     1321 ctgcgctcga cgagcaatga agtaattgcc gatttggaaa agcagattgc cgcgattctg
     1381 gacgaagaag caaagcgagc cggtatgact ctgaaaacgg atctgatctc gtcgtcaccg
     1441 gcggccgtat tgccaggcaa tcgtgagtcg cacctggtga agatcgctga agcagttcat
     1501 cgcgcgatgg gctttaatcc gccgattgga aacgctgggt cgaacaactc cagcgctgca
     1561 ctgttgcaag gaatcagttc gatatcgacc ggtgcgggcc cgtgcagtga tgcacatgcg
     1621 ttgacggaaa actgcgagat cgagccgctc tacaaaggca tcaaaaaggt tcttcttttg
     1681 gcagttgcgt tggcggggct gactcaatga ccttcacggc caatcatcac aaacccggtg
     1741 cgcaaaatta ttttgaggtc caacagcagc gaccagttct cgatgtagaa gaggtccaat
     1801 cgcaccatct cttcgaaagg cagacgattt ctgcctgaga cctgccacag gccagtcagc
     1861 cccggtttca tgtccagccg tttacgatgc cagagttcgt aagcttcaac ttcgtacgga
     1921 atcggtgggc gtgggccaac cacgctcatg tcgcccatca acacgttcag taattgcggc
     1981 acttcatcga gactcggtcg acgcaaaact tttcccaccc gcgtgatacg cggatcggcg
     2041 aagagcttgt aagtaggttt ctgcgcgttg ccgacattgg cttcagcacg tccggcaatg
     2101 aaggcccgtt ggtattcacg atgcagttcc ggatcggcat cggcgatcat cgtgcggaac
     2161 ttataaagaa gaaacagccg cccatcc
//
LOCUS       DASTEY010000529         5551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_506065, whole genome shotgun sequence.
ACCESSION   DASTEY010000529 DASTEY010000000
VERSION     DASTEY010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506065"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFO72_09295"
     CDS             complement(<1..405)
                     /locus_tag="VFO72_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9479529.1"
                     /translation="MPRSRRFFNAPSRRESLLPYLLVAPALVVLLALSIYPLLYSITI
                     SLQQETASGIVWTLGHFRRLASDNFFRTAMVHTFVYAIAALTCEFLLGLGLALLLNQQ
                     MRGRGLFRASLLVPMMLPTVVVGVVWRLMLNPN"
     gene            complement(368..1627)
                     /locus_tag="VFO72_09300"
     CDS             complement(368..1627)
                     /locus_tag="VFO72_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9479530.1"
                     /translation="MRLIIVLLLILLTLACGNSTSRQLTLAVVSGVEGEALKEAALDY
                     EAQTGVHIQIAQFPYANLFEKELIDLNARTGAYDLIMLDDPWFPLFAGSQFLTDLTPL
                     LQKRGQAGPDADFVESSVNVCRHPYQTGALYALPYVGNSQLFFYRKDLFAKHGLKEPS
                     TWDEVLAAAKTIQERETGADGGKMYGYVMRAAQGNAAVADFMPIFWAFGAEMFDPKGE
                     PAVNSPEGIAALEFMLQLGKYSPPGYASFNADEVSAHLLQGTAAMSINWPAWITAFSD
                     PSKSKVIGKMEFTTLPGARKPGQAEIGNWLIAIPRHSQNAEAAMDFLLWATNTEQMKR
                     SALRGNPPTRKSLFRDAELVAKYPAYPAQLRSLETSRPRPRTAQWNEIENAFGIFLSK
                     ANSGELSPAEAMNQANAEIEKILQRAQ"
     gene            1676..2680
                     /locus_tag="VFO72_09305"
     CDS             1676..2680
                     /locus_tag="VFO72_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HET9479531.1"
                     /translation="MTTGTGGVEVLKVQEKPDPQPHAGEVVIRVRAAGLNFADILSRQ
                     GLYPDSPPKPCVMGYEVSGIIEAVGDDINSSFVGKSVAALTRFGGQSDLVVVKATRVF
                     DKPESLTFEQAAAIPVNYLTAYALLVVMGSLHHGESVLIHNAGGGVGLAALDIAKKIG
                     ATTYGTASPSKHKFLAERGLDHAIDYRSQDWQPVLMELTNGRGVDLIIDPIGGAHWKK
                     SFASLRHTGRLGMFGVSTASANGLGGKLKMLKAVLQTPWFHGLPLLNRNRGVFGLNLG
                     HMWHEPEKIAVWMRDILRGVEEGWVRPYVDRSFSFEEVGEAHQYLEDRKNIGKVVLVP
                     "
     gene            complement(2669..3124)
                     /locus_tag="VFO72_09310"
     CDS             complement(2669..3124)
                     /locus_tag="VFO72_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479532.1"
                     /translation="MKIPIKYGLLTALALIVWVLVAHRLVANPNSLVHTLFGPIYFNI
                     LHFVMIFLGIKALERAKGEKPLFKEALKTGVSIAFVFALTASLFFVVVVLSVGTRWLA
                     IEPGAATTPTRLLLLQAFAGLFLGTMIFGLIYSTVIAFFVAKRQSESRH"
     gene            complement(3127..5301)
                     /locus_tag="VFO72_09315"
     CDS             complement(3127..5301)
                     /locus_tag="VFO72_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479533.1"
                     /translation="MLLTFPNLAPEDFAPWVNEGDDPAQQAQRWKDGLASWGQDGERI
                     QRLRDAADFRIYTPGSNAGIPVSILKSFDAPNEIIRHDNELLAERINTTVTSLLGLIG
                     ITADPLRSREHILISNILNQEWSNGRTLDIATLIHQIQSPSMTKIGVMQLDSFFPAED
                     RLELAMALNHLLASPSFASWMEGEPLDVQQLLHTSTGKPRVSIFSIAHLGDAERMFFV
                     SLLLNQVLGWMRTQSGTASLRAIVYMDEIFGYFPPVANPPSKLPLLTLLKQGRAFGLG
                     VVLATQNPVDLDYKGLANTGTWFIGRLQTERDKARVLEGLEGIAAGTGQKFDKQEMEQ
                     VLAGLTNRIFLLNNVHDDAQEVFETRWAMSYLRGPLTRTQIKLLMDPLESDITSPATV
                     TAPSPTQTVTTSTPPARQRSELSPEISQYYIPVRSASNATAKLWYQPMLLGVAEIHYS
                     KSKTIDVNEQVTLLAAISDGPVDLDWSAATPLNLPVEDLEAQPHSEAQFAEVPAKATK
                     AKSYMTWRKDFANWIYRNQRLELLDSPRLDIASNPGESERDFRIRLQQIAREQRDQAV
                     EKLRLRYAGKFAQLEERKRRAEQAVAREAEQAKDQKLQTAISFGATLLSSFMGRKRAK
                     MSTLGRATTAVRGVSRSMRQADDVDRAEENVASVMQKLADLEADFRAEAATLERSFDP
                     ATEPLDTLSLKPTKANINVKLLTLVWVPYWHDANGEPKPAWE"
BASE COUNT         1191 a         1528 c         1597 g         1235 t
ORIGIN      
        1 gtttggattc agcatcagac gccacacgac acccacgacc actgtgggca acatcatcgg
       61 caccagcaga ctcgcgcgaa acagtccacg cccgcgcatt tgttgattta gcaacaacgc
      121 caaaccaagc ccgagcagga actcgcacgt caaggctgcg atcgcataga cgaatgtatg
      181 gaccatcgcg gtgcgaaaga agttgtctga cgcgagccgt ctgaagtgac ccaaggtcca
      241 cacgatcccg gaggcggttt cctgttgcaa gctgatagtg atcgaataga gcagcgggta
      301 aattgaaagc gctagcagga ccaccagcgc cggagcgacc aggaggtaag gcagcagcga
      361 ttcgcgccta ctgggcgcgt tgaagaatct tctcgatctc ggcattcgcc tggttcattg
      421 cttccgcggg agacaactca ccactgttgg cctttgagag aaaaatgcca aaggcgtttt
      481 cgatctcgtt ccactgcgcc gtgcgcgggc ggggccgcga cgtttccagc gaacgaagtt
      541 gcgccgggta ggcgggatac tttgccacca gttcggcatc gcgaaagagc gatttccgtg
      601 tcggcggatt gccgcgcaga gccgatcgtt tcatctgttc ggtgttggtg gcccagagca
      661 ggaagtccat tgccgcttcc gcgttttgcg aatggcgtgg aatagcgatc agccagttgc
      721 caatctccgc ctggcctggt ttgcgcgcgc cgggcagcgt cgtgaattcc atcttgccga
      781 tcactttcga cttcgacggg tcgctgaacg cagtgatcca cgcgggccaa ttgatcgaca
      841 tcgccgccgt gccctgcaag aggtgcgcgc tcacttcgtc ggcattgaaa ctggcgtagc
      901 ccggcggtga gtacttaccc aactgcaaca taaattcgag cgcagcgatt ccttccggac
      961 tgttcactgc gggttcgccc ttcggatcga acatctcggc gccgaacgcc caaaagatcg
     1021 gcatgaagtc ggcgactgcg gcgttgccct gcgctgcgcg cataacgtag ccgtacatct
     1081 tgccgccatc tgcaccggtt tctctttcct gaatcgtttt ggcagccgcg agcacctcgt
     1141 cccacgttga cggttctttc agaccatgtt tcgcaaacag atctttgcga tagaagaaca
     1201 actgcgagtt gccgacgtaa ggcagcgcgt agagcgcgcc ggtttggtac ggatggcggc
     1261 atacgttgac ggaactctcg acgaaatcgg cgtctggtcc ggcttgtccc cgtttttgca
     1321 gcagcggcgt caggtcagtc agaaactggc tgccggcaaa aagtgggaac caggggtcat
     1381 cgagcatgat gaggtcgtag gcgccggtac gcgcattgag atctattaat tctttctcaa
     1441 aaagatttgc ataaggaaac tgtgctattt gaatgtggac gcccgtctgc gcttcgtaat
     1501 cgagtgcggc ttctttgagt gcttcgcctt cgactccgga aacgacggcc agtgtcagtt
     1561 gtcgagaggt ggaattcccg caagcgaggg tcagcaaaat aaggagcagt acaattatga
     1621 gccgcatatt tgcaacacag attaacactg ataacatgcc agcgtgcgac aaattgtgac
     1681 cacaggaacg ggcggcgttg aggttctgaa ggtccaggag aagccggatc cgcagccgca
     1741 cgcgggcgaa gtcgtgatca gagtcagggc cgcgggcctc aactttgccg acattctctc
     1801 gcggcaggga ctctatcctg attcgcctcc gaaaccctgc gtaatgggct acgaagtgag
     1861 cggcatcatc gaagcagttg gtgacgatat caacagttcg tttgtgggaa agtctgtggc
     1921 cgcgctgacg cgctttggcg gccaatcgga tctcgtcgta gttaaagcca cgcgagtgtt
     1981 cgacaaaccc gaatcactca ccttcgaaca ggcagccgct attcccgtga actacctgac
     2041 cgcttacgcg ctgctcgtcg tgatgggttc gcttcaccat ggcgagtcag tcttgatcca
     2101 caacgcgggc ggcggtgttg gcttagcggc gctcgacatc gctaagaaga tcggcgccac
     2161 aacttacggc accgccagtc cttcaaagca caaatttcta gctgagcgtg ggttggacca
     2221 tgcgatcgac taccgcagtc aggactggca gccggtgtta atggaactca cgaacggacg
     2281 cggcgtcgat ctcatcatcg atcccattgg cggcgcgcac tggaagaaga gttttgcttc
     2341 gttgcgacac accggacggc tcggaatgtt tggcgtttcg accgcttcgg ccaacggact
     2401 cggtggcaag ttgaagatgt tgaaggcggt gctgcaaacg ccctggtttc acgggttgcc
     2461 tttgctcaat cgaaaccgtg gcgtgttcgg tctgaatctg ggtcacatgt ggcacgagcc
     2521 ggaaaaaatc gcagtgtgga tgcgcgacat cctgcgcggg gtcgaggagg gatgggtgcg
     2581 gccttatgtc gatcggtcgt tctcattcga agaggtcggc gaagcgcacc agtatctcga
     2641 agatcgaaag aacatcggaa aagttgtttt agtgccttga ttcactctgg cgtttcgcga
     2701 cgaaaaaagc gatcaccgtc gagtagatca gtccaaagat catcgttccg agaaacaacc
     2761 cggcaaatgc ctggagcagc agcaatcgtg tcggcgtggt agcagcgccg ggttcaatcg
     2821 ccagccaccg cgtgcccacc gaaagaacga ccaccacgaa gaacaacgac gccgtcagcg
     2881 caaagacaaa tgcgatcgaa acaccggtct tcagcgcctc tttgaaaagc ggcttctcgc
     2941 ccttcgcgcg ttccagcgcc tttattccga gaaagatcat cacgaaatga aggatgttga
     3001 agtagattgg gccaaagagc gtgtgcacca gcgaattggg attcgcgact agccgatgag
     3061 ccaccagcac ccagacgatc aacgcgagcg ctgttaacag tccatacttg atcgggattt
     3121 tcatgtttac tcccacgccg gtttgggttc gccattcgca tcatgccagt aaggcaccca
     3181 gacgagcgtc agcagtttga cgttgatgtt tgctttcgtc ggtttcaggc tcaatgtgtc
     3241 gagtggttct gtggccgggt cgaaagaacg ctcgagagtc gcggcttccg ctctgaaatc
     3301 ggcctccaga tccgcgagct tttgcatcac ggaggcaacg ttctcctccg cacggtctac
     3361 atcatcggct tgtctcatcg atcgactgac gccgcgcacc gcggtcgtcg cacgaccaag
     3421 tgtcgacatc ttcgcgcgct ttcggcccat gaatgaagaa agtaatgtgg cgccaaacga
     3481 gattgcagtc tgtagcttct gatccttcgc ctgttccgct tcccgggcga ctgcctgttc
     3541 ggcgcgccgc tttcgctctt ccagctgggc aaacttgccc gcatatcgta ggcgtaactt
     3601 ttccaccgcc tggtcgcgtt gttcgcgcgc aatctgctgc aagcgaatgc ggaagtcgcg
     3661 ctcagattca cccggatttg aagcgatgtc caaacgggga ctatccaaca attcgagtcg
     3721 ctgattgcgg tagatccagt tggcaaagtc tttgcgccac gtcatgtagc tcttcgcttt
     3781 tgtggccttc gccggaactt ccgcgaattg cgcctcagag tgtggctgag cttcgagatc
     3841 ttcgaccgga agattgagtg gcgtcgccgc gctccagtct aaatcgactg gtccatcgct
     3901 gatggccgcg agcagcgtca cctgctcgtt gacgtcgatc gttttcgact tgctgtagtg
     3961 gatctcggcc acgccgagga gcattggttg gtaccagagc ttcgcggtcg catttgaagc
     4021 tgagcgaacg ggaatgtagt actgactaat ctcgggagag agttctgaac gttgtcgagc
     4081 cggtggcgta ctggttgtga ctgtttgggt tggggatgga gctgtgacgg tcgccgggct
     4141 cgtgatgtcc gactctagtg ggtccatcaa caacttgatc tgcgttcgcg tgagcggtcc
     4201 gcggagatac gacatggccc accgcgtttc aaacacttcc tgcgcgtcgt catgaacgtt
     4261 atttagaaga aagatccggt tcgtgagccc ggccagcacc tgctccatct cctgcttgtc
     4321 aaacttctgg cctgtgcccg cggcgattcc ttcgagacct tccaaaacgc gcgctttgtc
     4381 ccgttcggtc tgcaaccggc cgatgaacca cgtgcctgta ttcgcaagtc ctttgtaatc
     4441 gaggtcgacc ggattttgcg tcgcgagaac aactccgagc ccaaaagccc ggccttgttt
     4501 cagcagtgtc aacaacggca gcttcgaagg cggattcgca accggcggaa agtaaccaaa
     4561 gatctcgtcc atgtacacga tcgcgcgcag gctggcggtc cccgactgcg ttctcatcca
     4621 gcccaacacc tgattcagca gcagcgacac aaagaacata cgttccgcat cgcccagatg
     4681 cgcgatggaa aagatcgaca ctcgcggctt gccggtcgac gtgtggagca actgttgcac
     4741 gtcgagcggc tcgccttcca tccaactggc gaaactcggc gacgcgagca aatgattcaa
     4801 agccatcgcc agctccaaac gatcttcggc ggggaagaac gagtcgagct gcatgactcc
     4861 gatcttcgtc atggacggcg actggatctg gtgaatcagt gtggcaatgt cgagggttcg
     4921 gccgttggac cattcctggt tgagaatgtt ggagatcaga atgtgttcgc gactgcgaag
     4981 cggatccgct gtgatgccga tcagtcccag caggctcgta acggtggtgt tgattcgctc
     5041 ggcgagcagt tcgttatcgt gtctgatgat ttcgtttgga gcgtcaaacg atttcagaat
     5101 tgaaacaggg atacccgcat tactgccggg agtgtagatg cgaaagtcgg ccgcatcgcg
     5161 caggcgttgg atacgttcgc catcctgtcc ccagcttgcg agtccgtctt tccaacgttg
     5221 ggcttgctgc gcgggatcgt cgccttcatt gacccacggc gcaaaatcct ccggagcaag
     5281 gttggggaag gttagcagca agttactgag gtcgctgtgg gttcggtcat aaaatgaaat
     5341 ttgtacgtca taaaatgaaa tttgtaggtc ataaaatgaa atcgcgcgga ctagttacgc
     5401 tttattagtc gccgcccatt aatgaggtac tttcatctca atggtactca ccttcgctcg
     5461 tcacaacagt catcacatga cagcaacttc atactaagag atgagggagt tacgttgtga
     5521 tacctgctgc gacgtagctt gctttgacga c
//
LOCUS       DASTEY010000530         5113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_901980, whole genome shotgun sequence.
ACCESSION   DASTEY010000530 DASTEY010000000
VERSION     DASTEY010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901980"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..779
                     /locus_tag="VFO72_09320"
     CDS             96..779
                     /locus_tag="VFO72_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9479534.1"
                     /translation="MKSKILIVEDEPAMVAGLRDNFEYEGYEVISAEDGVTGLERALA
                     DSPDLVVLDVMMPRMSGLDVCKQLKAKRPGVPIIMLTARGQEIDKVVGLELGADDYVT
                     KPFSIRELMARVKAVLRRASAPASAPEIYRFSDVEVNIRSNEVRRNGELVDLSSKEFA
                     LLAYFIAHPVETLSRDRLLDAVWGYENYPSTRTVDTHIVHLRQKLEPNPEEPRFILTV
                     HGSGYKFVG"
     gene            889..1755
                     /locus_tag="VFO72_09325"
     CDS             889..1755
                     /locus_tag="VFO72_09325"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET9479535.1"
                     /translation="MTATALIAGLSPQRAVAKRVPVPTASREQLRELELKLETLQQQY
                     AELNTAIFEAAQVHRRLCAPSLVHYGPFDIASEIFAVRHLPGDFFTVEETKEGMVLAL
                     GDIGGKGLAAGMWVSHLAGLIRTRTAESSRPDAIVAGVNRDFARLSSVAPLSSLFVAR
                     LDAHRGTLDYCSAGHPPALLLRANGELETLSDGGPVLGVMPEASFDCGSVQLDSGDAM
                     LVCSDGILESFDALEQEFGRARLEAEFRRAHGGSAEAVLFSVLGAVQDFAAPRPLTDD
                     MTLVLVTRGRNV"
     gene            1761..2615
                     /locus_tag="VFO72_09330"
     CDS             1761..2615
                     /locus_tag="VFO72_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9479536.1"
                     /translation="MKFKGFLILCLLMLASASFVSAQEEPKYAKLAETKIHYVSEGKG
                     KDAVVLVHGWTCNLGHWRDQIPALSKRARVIALDLPGHGRSDKSQIKYDMDLFAAAIE
                     AVMKDANVERAVLVGHSMGTPVIRQFYRKYPQRTLALVIVDGGLRPFGTKEGREQMMA
                     PLRGPNYKDAGAQMFSAMAATLSEADKERVRSSFLSTPQHVLVSAMEALADESLYTTD
                     KINVPVLAILAKSPFWPADTEQFFRSLAPDLEYHMWDGVSHFLFMDKPKEFTAAVVSF
                     LDKKNLLK"
     gene            2651..3118
                     /locus_tag="VFO72_09335"
     CDS             2651..3118
                     /locus_tag="VFO72_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9479537.1"
                     /translation="MADVKPIPEGYHSVTPYLSINGAAEALEYYKKAFGATELFRMEH
                     DGKIGHAEIKIGDSPIMLSDEAPEMGFVSPKTLGGSPVGIMIYVEDVDTIFKQAIEAG
                     GVEKKPVQDQFYGDRSGTLTDPFGHVWFVATHKEDVTPEEMDKRLAAAKAKGA"
     gene            complement(3247..4503)
                     /locus_tag="VFO72_09340"
     CDS             complement(3247..4503)
                     /locus_tag="VFO72_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9479538.1"
                     /translation="MLTSANVAVDAVYRSDWGRIVATLIRQFGDFELAEDAAQEAFTA
                     AVDQWRSEGVPESPSAWIIQTAKHKAIDRLRRQTRLKEKLETHPEFFPTEIVDEPNFN
                     YGEIPDHRLRLIFTCCHPALALEAQVALTLRTLGGLQTDEIARAFLVPTTTMAQRLVR
                     AKRKIRDAGIPFTVPDTSDMPERLDAVLTAIYLIFTEGYAATRGQPLIRADLCAEAIR
                     LGRLVRNLLEPNRPPEASALVALMLLHDSRREARLDEAGDLVVLEEQDRGRWNKPQIA
                     EALALVDEAFSGTPGPFALEAAIAAEHCKAARAADTNWPRIVNLYDLLHQLLPSPVVA
                     LNRAVAVAMADGPRAGLELIDELSTSGELDEYHLLHAARADMLRRLGANAEAAESYEL
                     ALKLASNDSERRFLERRLREVRTPPS"
     gene            4594..4938
                     /locus_tag="VFO72_09345"
     CDS             4594..4938
                     /locus_tag="VFO72_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9479539.1"
                     /translation="MLLVYLDEHSLSEAEREQCYVESAQLARDLSARGKYLSAGPLHP
                     TATATSVRVREGRRLVTDGPFAETREQLGGYYLVEAHDLDEAMKIAERIPVAKYGTIE
                     IRPVLEISGLPA"
BASE COUNT         1102 a         1345 c         1576 g         1090 t
ORIGIN      
        1 gacgcggcag taaatttaca ctctcgctgc cgctcacggg tccagcccaa gctgccacgg
       61 ccggtacgac cggcactgcg taggaggcgc caatgatgaa aagcaagata ctcatcgtcg
      121 aagacgaacc ggcaatggtc gccggactgc gcgacaactt cgagtatgaa ggctacgaag
      181 tgatcagcgc cgaagacggc gtgacggggc ttgaacgcgc tctcgcagac tcaccggatc
      241 tcgttgtgct cgacgtgatg atgccgcgga tgagcggtct cgatgtttgc aagcagttga
      301 aggcgaagcg tcccggcgtg ccgatcatca tgttgacggc tcgtgggcag gagatcgaca
      361 aagtcgtggg cctcgaatta ggtgcggatg attacgttac aaaaccgttt tcaatccgcg
      421 agttgatggc ccgcgttaag gccgtgttga gacgcgcgtc cgcgcctgcg tccgcgcctg
      481 agatttatcg tttcagcgac gtcgaagtaa acattcgcag caatgaagtg cgccgcaatg
      541 gagagttggt cgatctgtcg tcaaaagagt ttgcgctgct tgcatacttc attgcgcacc
      601 cggtggagac actcagtcgc gaccgcttgc tggatgcagt gtggggatac gaaaactatc
      661 cgagtacacg aacagtcgat acgcacatcg ttcacttacg acagaagctc gaaccaaatc
      721 cggaagagcc ccgttttata cttaccgtgc atggtagtgg ttacaaattt gttggctagg
      781 gaagacttcc cgcaaaggcg cccaggcgca aagtagttct tggcgtttgc ttttgcgtct
      841 tgcgcctttg cgggagaact gcttgggtag tgaaaaggat ttcccgttat gactgcaaca
      901 gcacttatcg cagggctgtc tccccaacgc gcagtcgcga agcgcgttcc ggtaccaaca
      961 gcgagccgag agcagttacg cgaactcgaa ctcaagctcg aaacgcttca gcagcagtac
     1021 gccgagttga acacagcgat cttcgaggcc gcacaagtgc atcgtcgttt gtgtgccccg
     1081 agcctggtgc actacggccc tttcgacatt gccagcgaga tcttcgccgt gcgccacctg
     1141 cctggcgatt tcttcacggt ggaagaaacg aaagaaggaa tggtcctggc gcttggtgac
     1201 atcggcggca aaggactcgc cgcgggaatg tgggtcagtc atcttgccgg actgattcgc
     1261 acgcgaacgg ccgagagcag tcgtcccgat gccattgtcg cgggagtgaa tcgcgacttc
     1321 gcgcgtctgt cttcagtggc gccgttgagc agtctattcg tggctcgact cgacgcgcac
     1381 cggggaacgc tcgactactg tagcgcgggt catccgccgg cgttacttct gcgcgcgaat
     1441 ggcgaactgg aaacgctttc ggacggcgga ccggtgcttg gtgtgatgcc tgaagcctca
     1501 tttgattgcg gcagcgtgca attagactca ggcgatgcga tgctggtctg ctccgacggc
     1561 attcttgaat cttttgacgc gctggagcag gagtttggca gagcgcgact cgaagctgag
     1621 tttcggcgcg cgcacggcgg ctcggcggag gccgtgctgt tttcagttct cggcgcggtg
     1681 caggacttcg cggcgccacg cccgctcacg gacgacatga cgctcgtact cgttacgcgc
     1741 gggaggaatg tttgatcatg atgaagttca aaggtttttt gatcctatgt ttgttgatgc
     1801 tcgcgagtgc ttcgtttgtt tccgctcagg aagagccgaa gtatgcaaaa ctcgcagaga
     1861 caaagattca ctacgtaagt gaaggcaaag gcaaggacgc cgtggtgttg gtccatggct
     1921 ggacctgcaa cctgggtcac tggcgcgatc aaattcccgc actgtcaaag cgcgcgagag
     1981 tgattgcgct cgatttgccg ggtcacggac gtagtgacaa gtcgcaaatc aagtacgaca
     2041 tggatctgtt tgccgcagca atcgaggcgg tgatgaagga cgcgaacgtc gaacgagcgg
     2101 tgctggtcgg acacagcatg ggtactccgg taatccggca gttctaccga aaatatccgc
     2161 agaggacact ggcgctcgtg atcgtcgatg gcggattaag acccttcggc accaaagaag
     2221 ggcgcgagca gatgatggct ccattgcgcg ggccaaacta taaagatgcc ggagcgcaga
     2281 tgttctctgc catggcggcg accctgtcag aagctgataa agaacgggtc aggtcctcgt
     2341 ttctaagcac gccgcagcac gtgcttgtga gcgcgatgga ggcgctggcc gacgagtcgc
     2401 tctataccac tgacaagatc aatgtgccgg tgctggcaat tctcgcgaag tcgccgttct
     2461 ggcctgccga cacagaacaa tttttccgca gcctcgcgcc tgatcttgag taccacatgt
     2521 gggacggcgt gagtcatttc ctgttcatgg acaagccgaa ggaattcacc gcggcggtag
     2581 tctcgttcct ggacaaaaag aacctgttga aatagaacaa ctccaaaata aaagcgaaag
     2641 gggattagtg atggcagacg taaagccaat tccagaaggg tatcactccg taacgccgta
     2701 cctgagcatt aacggcgccg ccgaagcgct cgagtattac aaaaaggcgt ttggcgcgac
     2761 cgaactattc cgcatggaac atgacggtaa gatcggtcat gccgagatca agattggcga
     2821 ttctccgatc atgctctctg atgaggctcc ggagatgggt tttgtttccc cgaagacgtt
     2881 gggcggttct ccggtgggaa tcatgatcta cgtcgaagac gtcgacacga ttttcaaaca
     2941 ggcgatcgaa gcgggtgggg tggaaaagaa gccggtgcag gatcagtttt atggcgatcg
     3001 ctccgggacg ctgacggatc cgtttgggca cgtttggttt gtggcgacgc ataaggaaga
     3061 tgtgacgcca gaggaaatgg ataaacggct tgcagccgcc aaggctaagg gcgcttaagg
     3121 ttagggcgcg tttgttcctt ggaccgcagg cgtctcgcct acccggcagc gaaggaagct
     3181 agatacgaac gatgcaggcg agacgcctgc gatcccagga taaacgggtt gctggattga
     3241 gcgggtttag gaaggcggtg tgcggacttc gcggaggcga cgttcgagga agcggcgctc
     3301 gctgtcgttg cttgccagtt ttaaggcgag ttcgtagctt tccgccgctt cagcgttcgc
     3361 tccgaggcgc cgtagcatgt ctgcgcgcgc cgcatgaagc aggtgatatt cgtccaattc
     3421 gcctgacgtt gagagttcat ctatcaactc caaacctgct cgtggtccat cggccatcgc
     3481 caccgccacc gcgcgattca gtgcgacgac gggtgacggc agcagttggt gtaacaggtc
     3541 gtaaaggttg acgatccgcg gccaattcgt gtccgccgcg cgagcggcct tgcagtgctc
     3601 ggccgcgatc gcggcttcaa gtgcaaatgg accaggcgtg ccgctgaacg cttcatcaac
     3661 cagcgccaac gcttccgcga tctgcggctt gttccagcgg cctcgatcct gttcttcgag
     3721 cacgacgaga tcgcctgctt cgtccagtcg agcttcgcgt cgcgaatcgt ggagcaacat
     3781 caacgcgacc agcgcagagg cttccggcgg ccgattcggc tcgagcagat ttctgacgag
     3841 ccggccgagt cggatagctt ctgcgcataa gtccgctctg atcagcggct ggccgcgcgt
     3901 cgctgcgtaa ccttcggtga agatgagata gatagctgtc aacaccgcgt cgagacgctc
     3961 aggcatgtcg ctcgtgtccg gcacagtgaa ggggattccg gcgtcgcgga tctttcgctt
     4021 ggcacgcacg agacgctggg ccatcgtagt agtcgggacg agaaatgcgc gcgcgatttc
     4081 gtcggtttgc agtccgccga gtgtgcgcaa cgtcagtgcc acctgcgctt cgagcgcgag
     4141 cgcaggatgg caacaggtga agatcaaacg caggcgatgg tcgggaattt caccatagtt
     4201 gaaatttggt tcatcgacga tctcagtggg aaagaactcc gggtgcgtct cgagcttttc
     4261 tttcagacgc gtttgacgcc gcaggcgatc gatggcctta tgtttggctg tttggatgat
     4321 ccaggcgctt ggcgattcgg gaacaccttc cgatcgccac tgatcgacgg cggcggtaaa
     4381 cgcttcctga gcagcgtctt cggccagttc gaaatcgccg aactgccgga taagagtggc
     4441 gacaatgcgg ccccagtcag accggtagac cgcatcgaca gcgacattgg cactggtgag
     4501 cacgtttgat aataaaaaaa tttgtgcaaa cgtgtcgatt cgagaacaac tcgttcgact
     4561 tacggtttgg aggtggacat ttaagtgaaa tacatgctgt tggtttatct tgatgaacat
     4621 tcgttgagtg aagcagaacg cgaacagtgt tacgtggaat cggcccaact cgcccgcgat
     4681 ctgagcgcgc gagggaaata tctttcggcc gggccgttgc acccgaccgc gactgctacg
     4741 agcgtccgtg tgcgcgaggg aagacgcctc gtgacggatg gaccgttcgc tgagactcgc
     4801 gagcagcttg gcggttacta cttagtcgaa gcccacgatc tcgatgaagc gatgaagatc
     4861 gcggaacgca ttcccgtcgc caaatacggg acgatcgaaa tcagaccggt gctggaaatc
     4921 tcaggcctgc cggcttaaga ggagaataat gaacatacta ctttgggtca ttcaaatatt
     4981 gctggcgctg ctgtttttgt ttgccggcgg cacgaagctg gtgttgccgc ttgaaacgtt
     5041 gcagcaggcg ggatcgccga accaggttca acttcccgga ttattcatca ggttcatcgg
     5101 cgtgtgtgaa gtg
//
LOCUS       DASTEY010000531         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_440313, whole genome shotgun sequence.
ACCESSION   DASTEY010000531 DASTEY010000000
VERSION     DASTEY010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440313"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1369)
                     /locus_tag="VFO72_09350"
     CDS             complement(116..1369)
                     /locus_tag="VFO72_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9479540.1"
                     /translation="MGFEQGLEGRRVLFISYNGMLEPLGQTQVLPYLRELGKRGVRFT
                     LLSFERARAVTPEGAEKCEQLRRELQTQGIEWHWLRYHQRPSVPATIYDVVAGIRKAG
                     ALVRFNQIEMVHARGHIPATIALALKQRFGTKMIFDVRGLMAEEYVDAEHWREGSLPY
                     RLTKGTERRILAATDAVVTLTERIWPIIRDWEGLRGRSPYHEVIPCCVDLARFRVSDE
                     QRARRRQELGLADKFTIVYSGSLDGWYLTDKMADFFASFLQRNPQAHLLWLTTGSHDR
                     VRELMNSRNVGPDHYSVYSVAAAEVPSYLAAADTGLAFIKRCVSKLASSPTKNGEYLA
                     CGLPLIINAGIGDSDTLVDQWQAGVLVRDFTDEEYRITAEKIETMVAAPGAREKARRV
                     AEQLFHLERVGGERYASLYRRVLNS"
     gene            complement(1359..2390)
                     /locus_tag="VFO72_09355"
     CDS             complement(1359..2390)
                     /locus_tag="VFO72_09355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9479541.1"
                     /translation="MSSCAKRHNSQMKVSVIVPVRDEEHSIRELLDSLLKQTRPPDEI
                     VITDGGSVDATPQIIEAYIQRGAPVRLLRAGPALPGRGRNLGAAAAQFEWLGFTDAGI
                     RLENNWLEMLVARAAQDPAIDIVYGSWEPMTDTFFKECAAIAYVPPPAPRDGIVVRPR
                     FVASTLLRRDAWAAVHGFPEDLRSAEDLVFMDRLEAAGYRAVFEPRARVHWNLRSTLS
                     STFKRFLVYSRNNIRAGLWRQWQATILFRYAVLLVLLVIALIMDPRWALVPVVAWLVM
                     LAARAAVSIRRNRHCYPASLFRNLLRGGLIMSLLAVLDAAALLGSIQWLLFDSFRSTG
                     KTVEAGNGV"
     gene            complement(2577..>3357)
                     /locus_tag="VFO72_09360"
     CDS             complement(2577..>3357)
                     /locus_tag="VFO72_09360"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9479542.1"
                     /translation="NMGGIGFIENNKAVIVRDNTLINLNSIEAARINGVKRYLYTSSA
                     CIYPGYKQKETDVTPLKEEDAYPADAEDGYGWEKLYMERVCRHYTEDFGLETRVVRFH
                     NIFGPLGTYDGGREKSPAAICRKVALAQDGDDIEVWGDGEQTRSYCYIDDCVEGIYRL
                     MKSDHREPINLGQDRMISVNELVDMVAKIAGKKIGKRYDLTKPQGVRGRNSDNTRLRQ
                     VLNWEPSVSLEEGMAVTYHWIAGQLEKQASHPQSAQMLTSV"
BASE COUNT          742 a         1059 c          857 g          699 t
ORIGIN      
        1 ttccctggga ccgcaggcgt ctcgcctgca actcgtcgta acgggctacc tgcgcaacac
       61 agcaggcgag acgcctgcgg tcccagggaa caagacgccc tgcggtccag cgtcgtcagc
      121 tattcaacac tctccgataa agcgacgcat atctttcacc accaaccctc tcgaggtgaa
      181 aaagctgctc cgccactcgc cgcgccttct cccgcgcacc cggcgccgcg accatcgttt
      241 caatcttctc cgcggtaatc cggtattcct catccgtaaa atctctaacc agcacgcccg
      301 cctgccactg gtctacgagc gtgtcagagt cgccaatccc tgcattgatt atgagcggca
      361 acccacacgc cagatactcc ccgttttttg tcggcgatga cgcgagcttg gatacacagc
      421 gcttgataaa cgcaagcccg gtatctgccg cagcaagata cgaaggaact tccgcagccg
      481 caaccgagta gacactgtaa tgatccggcc caacattccg gctattcatc agctccctca
      541 cgcgatcatg gctgcccgtc gtaagccaca aaagatgtgc ctgcggattc cgctgcaaaa
      601 agctggcaaa gaagtcggcc atcttgtccg tcaaatacca gccatccagc gaaccgctgt
      661 agacaatcgt aaacttgtcc gccaacccga gttcctgacg ccttcgcgca cgctgttcat
      721 cgctgacccg gaaacgagcg agatcgacgc aacagggaat aacttcgtga tacggcgagc
      781 ggccgcgcag cccttcccag tcacggatga tgggccaaat tctttccgtc aaagtgacga
      841 cggcatcggt cgccgccaga attcgccgct cagtgccttt cgtaagtcga taaggcaagc
      901 tcccttcgcg ccagtgttcc gcgtcgacgt actcctcggc cattaacccg cgcacgtcga
      961 agatcatttt cgttccaaaa cgctgcttca acgcgagcgc gatggtcgcc ggaatgtgac
     1021 cgcgcgcgtg gaccatctcg atctgattaa atctcaccag cgcacctgct ttgcgaatgc
     1081 ctgccactac gtcgtagatc gtggcaggaa ctgaaggccg ctgatgataa cgcagccagt
     1141 gccactctat gccttgcgtc tgaagttcgc gccgcagttg ttcacacttt tcagcgcctt
     1201 caggtgtgac cgcgcgcgcg cgctcaaaac tcagcagcgt gaagcgaaca ccccttttcc
     1261 ccagttcccg caaatacgga agcacctgag tttgtcctaa aggctcaagc attccgttgt
     1321 aggagatgaa gagtacccgc ctgccctcga gcccctgttc aaaccccatt gccggcctcc
     1381 accgtcttgc cagtagaacg aaacgaatcg aacaataacc actgaatgct gccgagtaac
     1441 gccgccgcat ccagcaccgc gagcagcgac atgattaaac cgccgcgaag taggtttcga
     1501 aaaagtgacg ccggataaca gtggcggtta cgcctgatcg acaccgcagc acgcgcggcc
     1561 aacataacca gccacgcaac cacaggcacc aaagcccaac gcggatccat gatcagtgca
     1621 atcaccaaca acacaagcag caccgcgtag cgaaacagta tcgtcgcttg ccactgccgc
     1681 cagagcccgg cgcggatgtt gttgcgcgaa tagaccagaa accgcttgaa cgtcgaagag
     1741 agtgtcgacc gcaggttcca atgcacccgc gcacgcggct caaacacggc tcgataacct
     1801 gctgcttcaa gacggtccat gaagacgaga tcttccgccg agcgcagatc ttcgggaaac
     1861 ccgtgcactg ctgcccatgc gtcgcgtcgc aacaacgttg acgcaacgaa acgcggacgc
     1921 acgacgattc catcacgcgg cgcgggcggg ggaacatatg cgatcgctgc gcactctttg
     1981 aaaaatgtgt ccgtcattgg ttcccacgaa ccgtagacga tatcgatcgc cgggtcctgc
     2041 gccgcgcgcg caacaagcat ctcgagccag ttattctcca gacgaatgcc ggcatcagtg
     2101 aatcccaacc actcaaactg cgccgcggcc gcgccaagat tgcgtcctcg tccgggcaat
     2161 gcaggacctg cccgaagcaa tcgcacgggc gcgcctcgct gaatgtatgc ttcgatgatt
     2221 tgaggtgtgg cgtcgaccga accaccgtcc gtgatcacga tctcgtccgg cggacgcgtt
     2281 tgcttgagca ggctgtcgag caactcgcga atggagtgct cttcatccct caccgggaca
     2341 ataacgctga ctttcatctg agagttatgc cgctttgcgc aagaactcat ctcattctca
     2401 tccggcttca gccgggtgtc gcgcttgcca cgtaaggcag caaaccgttt caacggttta
     2461 gcgttttcgc acgccaaacc gttaaaacgg tttctgacca gaagccttgc cggtgcaccc
     2521 ggctaaagtc gggtgagaat gaaatgactt tcgggcaatg cccagttatc cactgattac
     2581 acagatgtca gcatctgtgc gctctgtgga tggctcgcct gcttctcgag ttgaccggcg
     2641 atccagtggt aggtaaccgc catgccttct tccaacgaaa cagacggctc ccaattcaaa
     2701 acttgccgca agcgtgtgtt gtcgctgttt cgacctctga ctccctgggg cttcgtcaga
     2761 tcgtaacgtt taccgatctt cttgccggca atcttcgcca ccatgtcgac cagctcgttg
     2821 acggagatca tccggtcctg gcccaggttg atcggctcgc gatggtcgct tttcatcagg
     2881 cgatagatgc cttccacaca gtcgtcgatg taacaatagg agcgcgtctg ttcgccgtcg
     2941 ccccaaactt caatatcgtc gccgtcctgg gccaacgcaa ccttgcgaca gatagcggcc
     3001 ggtgattttt cgcgcccgcc gtcgtacgtt cccaatggcc caaagatgtt gtgaaacctg
     3061 acgactcggg tttcgagtcc gaaatcttcc gtgtagtgac ggcaaacgcg ctccatgtaa
     3121 agcttttccc aaccgtaacc gtcttccgcg tcggccggat atgcatcctc ttcttttaat
     3181 ggcgtgacgt cggtttcctt ctgtttgtaa ccgggataga tacaggcgct ggaagtgtag
     3241 agataacgtt tgacgccgtt gatgcgcgcg gcttcgatcg aattgagatt gatcagcgtg
     3301 ttgtcgcgaa cgatgacggc tttgttgttt tcgatgaagc caatgccgcc catgttt
//
LOCUS       DASTEY010000532         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_604719, whole genome shotgun sequence.
ACCESSION   DASTEY010000532 DASTEY010000000
VERSION     DASTEY010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604719"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFO72_09365"
     CDS             complement(<1..506)
                     /locus_tag="VFO72_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479543.1"
                     /translation="MGVAKLTDANTIVPESKYARVERERRYLLSDLPEGLTRADPHLQ
                     ITDNYITGTRLRLRKVREPRTNKWTGKFTQKFAPNSADLSRMVITNTYLNALEAETMS
                     VFNSNEIRKNRYRFEFEGRTFAIDMFLGDLFGLVLAEVSFDTDEELDSFPKPPFALAD
                     VTNDPVFTG"
     gene            complement(526..1470)
                     /locus_tag="VFO72_09370"
     CDS             complement(526..1470)
                     /locus_tag="VFO72_09370"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HET9479544.1"
                     /translation="MSSHNRSCSLLAALCVLCFAAAAFAQISTPRASQKASVMQRIGV
                     TDVTITYSRPGVKGRKIWGDPLPEQTAKGEATLDNQMERPKGAPIVPWGHMWRTGANE
                     ATQFVVTDEVLINGQKLPAGSYSLHTIPNKDEWTIVFNGTANQWGSFNYDPAKDTLRV
                     KAKPESLPTNQEWMSFTIDPVTEDSAQVNIRWEKISVPFTVKVPDVAALTLSKLKTTV
                     GEAKPDDWRTPMQAGMYLMNNDNAEDDAQGMAWLDQSIKVKETFQNLSNKANILYKLG
                     KKEEAIALAEQAIQRGKADKVDTTNFEKRLADMKAGKI"
     gene            1565..>2615
                     /locus_tag="VFO72_09375"
     CDS             1565..>2615
                     /locus_tag="VFO72_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479545.1"
                     /translation="MSRSRPTRPFLCLLVLGLTFGAIALEAKAQNGHGGPTRLLRTPT
                     VSSTQIGFAYANNIWVAPRAGGSARRLTSFQGQTANPQFSPDGRWIAFSGEYAGNFDV
                     YIVPSDGGEPKRLTWHPGADLVQGWSPDGRTILFSSGRGTWSPGGAPRFWTVPAEGGV
                     EDPMALPRGYQGKISPDGTRIAYRMNNSWDEERRNYRGGQNRPIWIVDLKTYDLVSPP
                     WTESKDVDPVWVEDSVFFISDRDGVANVWEYQTKSKKLTQITKFTDFDVKSLDSGAGM
                     VVFEQAGYVHELDPKNGKTKIVNITATGDFPWMMPNWEDVTNRLTNIALSPTGRRVAV
                     EARGEIFTIPAEKGDI"
BASE COUNT          546 a          764 c          697 g          608 t
ORIGIN      
        1 cccgtgaaca ccggatcgtt cgtaacatca gcgagcgcga acggcggttt cggaaaacta
       61 tccagctctt catccgtgtc aaaacttact tccgccagca ccagtccaaa caagtcgccg
      121 agaaacatat caatcgcaaa cgtccgcccc tcaaactcga aacgataccg gttcttccgg
      181 atctcgttgc tgttgaagac tgacatcgtt tcagcttcga gcgcatttag atacgtgttg
      241 gtgataacca tacgcgacag gtcagccgaa ttcggcgcaa acttctgcgt gaacttcccg
      301 gtccatttgt tggtccgtgg ttcgcgcacc ttgcgcaatc tgagccgcgt gccggtgatg
      361 tagttgtcag tgatctgaag atgtggatcg gcgcgggtca aaccttcggg cagatctgat
      421 aacagatagc gccgttcgcg ttcaacccgc gcgtacttgc tctcgggaac gatcgtattt
      481 gcgtctgtaa gtttggctac ccccatccct aaatctggct tcggcctaaa tcttcccggc
      541 cttcatgtcg gccagacgct tttcgaagtt cgtggtgtcg accttgtcgg ccttgccgcg
      601 ctgaatcgcc tgctcggcca gtgcaatcgc ctcttctttc ttgcccagct tgtataggat
      661 atttgccttg ttcgaaagat tctggaaggt ctctttcact ttgatcgact gatcgagcca
      721 cgccatgccc tgcgcatcat cctcggcgtt gtcgttgttc atcagataca ttcccgcctg
      781 catcggcgtg cgccagtcat ccggcttggc ctcaccaaca gtggtcttga gtttcgacaa
      841 ggtgagcgca gccacgtccg gcacctttac ggtgaacgga acactgatct tctcccaacg
      901 aatgtttacc tgcgcggaat cttcggtcac cgggtcgatt gtgaaagaca tccactcctg
      961 atttgtcggg agcgattcgg gtttcgcttt cacgcgtagc gtatcctttg ccggatcgta
     1021 attgaaactg ccccactggt tcgcggtgcc gttgaaaacg atcgtccact cgtctttatt
     1081 tggaatcgtg tgaagactat aactgccggc tggaagcttc tgtccgttga tcaaaacttc
     1141 gtcagtgact acaaactggg tcgcttcgtt cgcgccagtg cgccacatgt gtccccacgg
     1201 aacaatgggc gcgcctttgg gccgttccat ctgattatcg agcgttgcct cgccttttgc
     1261 ggtctgttcc ggcaacggat cgccccaaat cttgcggcct ttgacgccgg gacggctgta
     1321 agtgatcgtc acgtcagtga cgccaattct ttgcatcaca ctcgccttct ggctcgcacg
     1381 cggcgtcgag atctgcgcga acgcagcggc ggcgaagcac aaaacacaga gagcggctaa
     1441 gagactgcaa ctgcgattat gggaactcat ggtctttctc ctcttggaaa gggttttgcg
     1501 aataatacaa tgtggtattc tttgcgccaa ttctaggcca ccacgtttcg gaggacaaac
     1561 ccatatgtca cggtctcgcc caacgcgtcc ctttctctgt ctgctcgtct taggtctaac
     1621 tttcggcgct atcgcgctcg aggcaaaagc acaaaacggc cacggcggcc cgacacgact
     1681 gctgcgcacg ccaactgtca gctcaactca gatcggtttc gcctacgcaa ataacatctg
     1741 ggtcgctccg cgcgccggcg gcagcgcgcg ccgtttgacg agttttcagg gacaaactgc
     1801 caatccacaa ttctcgcctg atgggcgctg gattgctttc agcggcgagt acgccgggaa
     1861 ctttgacgtc tacatcgtgc cttctgatgg cggcgagcca aaacgattaa cgtggcatcc
     1921 gggcgcggat ttggtgcaag gctggtcacc ggatggtcgc acgattctct tttcatccgg
     1981 ccgcgggacc tggtcgcctg gcggcgcgcc gcgattctgg actgtgccgg ctgaaggcgg
     2041 cgttgaagat ccgatggctc tgccgcgcgg ctatcaagga aagatctcgc cggacggcac
     2101 acgcatcgct taccgaatga acaattcgtg ggacgaagaa cggcgcaact atcgtggcgg
     2161 acagaaccgg ccgatctgga tcgtggatct gaaaacctac gacctcgttt caccaccgtg
     2221 gaccgaatca aaggatgtcg atccggtgtg ggtcgaagat tcggtcttct tcatctccga
     2281 ccgcgacggc gtcgcaaacg tctgggaata tcaaacgaag agcaagaagc tcacgcagat
     2341 cacgaagttc acggactttg acgtcaaatc gctcgactcc ggcgcgggca tggtcgtgtt
     2401 tgagcaagcc ggctacgttc acgaactcga cccgaagaac ggcaaaacca aaatcgtgaa
     2461 catcaccgcg accggcgact tcccatggat gatgccaaac tgggaagatg ttactaaccg
     2521 tctaacaaac attgcgttgt caccgaccgg cagacgcgtt gccgttgaag cacgcggcga
     2581 gatcttcaca attcccgccg agaagggcga catcc
//
LOCUS       DASTEY010000533         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1396185, whole genome shotgun sequence.
ACCESSION   DASTEY010000533 DASTEY010000000
VERSION     DASTEY010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396185"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFO72_09380"
     CDS             complement(<1..814)
                     /locus_tag="VFO72_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479546.1"
                     /translation="MADLVMMQFEITVPLPGTAGVSPASHGSAQLVTLQAGRLRSQHK
                     GASVFKLHQYLAIWVIWAGVFGVGSAEARPQVKVRVSLPAEVRINIESMPPAQEWSFR
                     NAYAGLLGIAERVAEFQALGANGDRVAAKKIAVGEFRSERDVTAISYVVKIPPPRASD
                     VAHVSWIGGKSGFLMPGDLLPVQLNDVVIEFSLPSGWTSQATIDADRDGKYEVAEPET
                     EVFFVGSSLRRQSKRVEGVELQSVVSGEWPFKDSVVLNSASKVVEKYLRLTGF"
     gene            892..1260
                     /locus_tag="VFO72_09385"
     CDS             892..1260
                     /locus_tag="VFO72_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479547.1"
                     /translation="MFALIKNQSSTFARWLNLLLVVFILYGTTVESAHRHGRILLSPA
                     GATSQIDNEQTKNLASNRSGCNDCLICQLHQSFTTTLIALRLSDPPAQLPQRVITVVP
                     RDLLSHIVSPLAGRAPPFVS"
     gene            1328..>3207
                     /locus_tag="VFO72_09390"
     CDS             1328..>3207
                     /locus_tag="VFO72_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9479548.1"
                     /translation="MFTRSILLSTIFLVSISLPVLAQSTTATLRGTVTLGDGKPVHHV
                     LVTILQLKRSTDTDESGKYEFTNVPPGRYDVLAHLDRVPDVVQTIELGAGAAATLDFK
                     IELSGIREQVNVTATGSEQALSSSIQSVDVIGSVDLAKKSPVSLGEALDGELGVSKRS
                     FGPGTARPVIRGFDGDRVLVLQDGNRIGGLGFESGDHAEPVDVLTVERIEIVKGPATL
                     LYGSNAIGGVVNTISGHDAPHHGFNGYVTGLGSTNSSQGGGSGGFEYGTYKWLVWGNA
                     GGQRSGDYDTPIGKVRNSFSREASAAAGFGYYPKKAFFSFNYNFNKRRYGIPVDPNEE
                     DAEIVELNPRRHSVEVRGGFRENPSFFQAGTFSVQYNNYRHAEIESLTGELGTQFKND
                     SVVYQAMFDQRKIGRLSGRFGFWGQHRDFSAIGEEALAPPTKQNAFAVFGLQNLDFER
                     VALQFGARLETNRYRPEVTETRGVLPDRNFTGFSGAAGIRVNTWRGGALVANYSHSYR
                     APSLEELYNFGPHPGNLAFEIGNPNLVRERGNGLDFGVRHSSKRLRFEANGFYYHIND
                     FVFLAPAGDVEEGLIVADYAQATTRYAGAEARFDAALHSSLWLNLGLDYVNADLTDTN
                     TPL"
BASE COUNT          734 a          978 c          822 g          673 t
ORIGIN      
        1 tgaaccctgt aagccgcagg tatttctcaa ccaccttact tgcagaattc aatacgacgg
       61 agtccttgaa cggccactca ccagatacga cagactgcaa ctccacgcct tcaacacgct
      121 ttgattgccg gcgcagcgaa ctgccaacga aaaaaacttc ggtttcaggt tcagcaactt
      181 catactttcc gtcgcggtcc gcatcgatcg tcgcttgaga agtccaacca gaagggagcg
      241 agaattcgat cacaacatcg ttgagttgca ccggcaacag atcgccaggc atgaggaacc
      301 ctgacttgcc gccgatccaa ctcacgtgcg cgacatcgga tgcgcgcggc ggtggaatct
      361 taacaacgta actgatcgcc gtaacatctc gttccgatct aaactcaccg acagcgatct
      421 tcttcgcggc gactcggtcg ccattcgcgc ctaacgcctg aaattccgca actctctccg
      481 caatgccgag caatcccgca tacgcattgc gaaaagacca ctcctgcgcc ggcggcattg
      541 actcgatgtt aatcctgacc tcagcaggca aactgactct gaccttaacc tgcggcctcg
      601 cctcagctga accgacgcca aacacaccgg cccaaatgac ccagatcgcc aggtactgat
      661 gtagtttgaa aactgaggcg cctttgtgct gggaccgcag gcgtcccgcc tgcagggtga
      721 cgagttgggc gctgccgtga gatgcaggcg agacgcctgc cgtcccaggt aaaggaaccg
      781 taatttcaaa ctgcatcatt accagatcgg ccatgcagaa ttgcgtttct aagccgcctc
      841 tcagtacaat tcctccgctc cctgaatcta acacaaccag tacggaccag catgtttgct
      901 ctaatcaaga accagagctc cacattcgcg cgttggctca atctgctgct ggtcgtcttt
      961 attctttacg gcacaactgt cgagtctgcc caccgccacg gtcgcatatt gctctcaccc
     1021 gcgggtgcaa cttcgcagat cgataacgaa cagaccaaaa atctcgcaag taacagaagc
     1081 ggttgcaacg actgtctcat ctgccagttg caccagagct tcaccaccac attaatcgct
     1141 ctgcggctaa gcgatccgcc ggcacaactg ccgcaacgag taatcaccgt cgtcccgcgc
     1201 gatcttctgt cgcacatcgt cagccctctc gccggacgag ctccaccgtt tgtctcctaa
     1261 tccaactcga ttcgactaca ccattagtgg gttcgactgc gcctgcgcgc gcctggagat
     1321 aaaccttatg tttactcgaa gcattcttct ttcaacgatt ttccttgttt ctatcagttt
     1381 gcctgtattg gcccaatcca ccaccgctac gctgcgcggc accgtcacgc tgggcgacgg
     1441 taaacccgtc caccacgtcc tggtgacgat cctgcagctc aaacgcagca ccgatacaga
     1501 cgaaagcggt aaatacgaat ttaccaatgt gccgccgggt agatacgacg tcctggcgca
     1561 tctcgatcgc gtacccgacg tcgtacagac gatcgaattg ggcgcaggcg ccgccgctac
     1621 tctcgacttt aagatcgaac ttagcggcat acgcgaacag gtaaacgtaa ccgccaccgg
     1681 ctcagaacag gcgctcagta gttcaatcca atcggttgac gtcatcggct ctgtcgatct
     1741 cgcaaagaag agtccggtct cgctcggcga agctctcgac ggtgaactcg gcgtgtccaa
     1801 acgaagtttc ggaccgggca cagccagacc ggtgattcgc ggttttgacg gcgatcgcgt
     1861 gctcgtactc caggatggaa atcgcatcgg cgggttgggt tttgaatcgg gcgatcacgc
     1921 cgagcctgta gacgtgttga cagttgagag aattgagatc gtcaaaggcc cggcgactct
     1981 tctttacggc agtaatgcga ttggcggtgt tgtcaacacg atttccggac acgacgcgcc
     2041 acaccacggc ttcaatggtt acgtgaccgg tttgggaagc accaacagct cgcagggagg
     2101 cggcagcggt ggtttcgagt acggcacgta caaatggctc gtctggggca acgccggcgg
     2161 acaaagatcc ggcgactacg acacgccgat cgggaaagtg cggaactcat tttcgcgaga
     2221 agcaagcgcc gccgctggct tcggctacta tccgaaaaag gccttcttca gttttaacta
     2281 caacttcaac aagcgacgtt acgggattcc cgtcgatccg aatgaggaag atgcggagat
     2341 cgtcgagctg aatccacgcc gccacagcgt cgaggttcgt gggggctttc gcgagaaccc
     2401 ctcgttcttt caagccggaa ctttctcggt gcagtacaac aattaccggc acgcggagat
     2461 tgagtcgctg accggtgaat taggcacgca gttcaaaaac gattccgtcg tctaccaggc
     2521 gatgttcgat cagcgaaaga ttgggagact ctccggacgc ttcggtttct ggggccagca
     2581 ccgcgacttc tccgctatag gcgaagaagc gctggcgccg ccgacaaagc aaaatgcctt
     2641 tgcagtcttt ggattgcaaa atcttgactt cgaaagagtg gcgttgcagt tcggcgctcg
     2701 gctcgaaact aatcgctatc gaccggaagt taccgaaaca cgcggggttc tccccgacag
     2761 aaacttcacg ggcttttccg gtgcggccgg cattcgcgta aacacgtggc gaggcggcgc
     2821 gctcgttgcc aactattcac attcatatcg cgcgccatcg ctcgaagagc tctacaattt
     2881 tggaccgcac ccgggtaatc tcgcgtttga aatcggcaac cccaatctcg tgcgcgagcg
     2941 tggcaacggt ctcgacttcg gcgtgcgcca ctcgtccaaa cgtttgcggt tcgaagcaaa
     3001 cggcttctat taccacatca acgacttcgt ctttctcgct ccggcaggag atgtcgaaga
     3061 gggcctaatc gtcgccgact acgctcaggc aacgacgcgc tatgcgggcg ccgaagcgcg
     3121 tttcgatgcc gcactgcact catcactctg gctcaatctc ggactcgact acgtcaacgc
     3181 agatctcacc gacaccaata ctccgct
//
LOCUS       DASTEY010000534         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_126855, whole genome shotgun sequence.
ACCESSION   DASTEY010000534 DASTEY010000000
VERSION     DASTEY010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126855"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFO72_09395"
     CDS             <1..450
                     /locus_tag="VFO72_09395"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET9479549.1"
                     /translation="VLRQLQKAKMHFGFVVDEHGGLEGIITLEDLLEEIVGEISDEHD
                     EEVNEQITEIDGRTFVLAGGLAVRDLNRRLKTSVPESETYTTIGGFLMTEAGHVLKPG
                     EVVTYDGLVFHVERVEKRRVMRVKLELLETNGEGEPQDELDRARSSA"
     gene            419..1717
                     /gene="eno"
                     /locus_tag="VFO72_09400"
     CDS             419..1717
                     /gene="eno"
                     /locus_tag="VFO72_09400"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9479550.1"
                     /translation="MSWIEHVLAREILDSRGNPTVEAEVVLADGEIGRAAVPSGASTG
                     ENEAVELRDKDERRYGGKGVLKAVHNVNRVIAPALEGLDALDQAEVDRVLIELDGTKT
                     KKTLGANALLAVSLATARAAALHLELPLYRYLGGPNSRTLPVPMMNIINGGAHADNNV
                     DFQEFMIVPVGAPNFSEALQIGAEVFHTLKELLKGKKYATSVGDEGGFAPNFRSNEEA
                     IETVVAAIEAAGYGPGTDCLLALDPAASEFYKNGVYVFEKSDERKLTSEEMVKYWKTW
                     CDNYPIISIEDGMAENDWDGWKLLTDELGDRVQLVGDDLFVTNTEFLQKGIDLGVANS
                     ILIKVNQIGTLTETLDCIELAQRNKRTAIISHRSGETEDPFIADLAVATNAGQIKTGS
                     LSRTDRIAKYNQLLRIEEDLRDASRYPGRKAFHQIEVRFE"
     gene            1710..>3069
                     /gene="gpmI"
                     /locus_tag="VFO72_09405"
     CDS             1710..>3069
                     /gene="gpmI"
                     /locus_tag="VFO72_09405"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET9479551.1"
                     /translation="MNKKRGPLALIIIDGWGYSPARDGNAIALAALPFYDELCEKYPQ
                     TLLEAHGLRVGLPAGIMGNSEVGHLNIGSGRVIRMDVSLVDHEIETGEFFRNNVLTGA
                     IERARDRGKALHLMGLCSDGQVHSSLTHLYALLKLARDLEMQRVYIHCFLDGRDTPPS
                     SAVNYLSALENKAGEIGCGQIATVTGRYYAMDRDKRWERTKRAYDLLVHGIGERTTDP
                     VKAARQSYERGVTDEFVEPISIVRATMEPVATIKDGDSVIFFNFRPDRARQLTRALTV
                     SRFNEFEVSERPKVDFVCFTMYDRTFDLPIAFPPREHHNVLAEVWSKVCVRNYRLAET
                     EKYAHVTYFFNGGIEKEFECERRLLVPSPKIATYDLQPEMSAFKVTDQVLHGIDEGET
                     DVFVVNFANPDMVGHTGKLDKTIEACQYVDTCLGWITKRMRDARGTTIITADHGNAEQ
                     MID"
BASE COUNT          741 a          806 c          895 g          627 t
ORIGIN      
        1 gtcctgaggc agttgcagaa agcaaaaatg cattttggtt tcgtggtcga cgaacacggc
       61 ggcctggaag ggatcatcac gctcgaagac ttactcgagg agatcgtggg cgagatttcc
      121 gacgaacacg acgaagaagt aaacgaacag attaccgaga tcgatgggcg tacgtttgtc
      181 ctcgcgggtg gattggcagt gcgcgatctc aaccggcgtt tgaaaacttc tgtaccggag
      241 tcggagacct acacgaccat cggtggcttt ttaatgactg aagcgggaca cgtactcaag
      301 ccgggcgaag tggtgacgta tgacgggctt gtctttcacg tcgagcgcgt tgagaagcgg
      361 cgcgtgatgc gcgttaagct ggagctttta gaaactaacg gagaagggga accacaggat
      421 gagctggatc gagcacgttc tagcgcgtga aatacttgac tctcgcggca atccgactgt
      481 tgaagcggaa gtcgttttgg ctgatggcga aatcggccgc gccgcggtgc cgtcgggagc
      541 ttcaaccggt gagaatgagg cggtcgaact gcgtgacaaa gatgaaagac gttacggcgg
      601 caagggcgtg ttgaaggccg ttcataacgt caaccgggta attgctccgg cgctcgaagg
      661 actcgacgca ctcgatcaag cggaggtcga tcgagtgttg attgaactcg acggtacgaa
      721 aacgaagaaa acactcggcg ccaacgcgtt gctggctgtg tcgctcgcga ccgcgcgagc
      781 ggcggcgttg catctcgagt tgccgctcta tcgttatctt ggtggtccaa actcgcgcac
      841 gcttccagtg ccgatgatga acatcatcaa cggcggcgcg cacgccgata acaacgtcga
      901 tttccaggag ttcatgattg ttccggtcgg cgcgccaaat ttcagtgaag cgttgcagat
      961 cggcgctgag gtgttccaca cactcaaaga actactgaag ggcaaaaagt acgcgaccag
     1021 cgtcggcgac gaaggagggt tcgcaccaaa ctttcgctcc aatgaagagg cgatagaaac
     1081 tgtcgtcgca gcaatcgaag cagcgggcta cggccccgga actgattgcc tgctggcgct
     1141 cgatccagcc gcgagcgagt tttacaaaaa cggagtttac gttttcgaaa aaagcgacga
     1201 aagaaagttg accagtgagg agatggtgaa gtactggaaa acctggtgcg acaactatcc
     1261 gatcatctcc atcgaagacg gcatggctga gaacgattgg gacggttgga aattgcttac
     1321 cgatgaactc ggcgaccgag tgcaactcgt tggcgacgat ctcttcgtca ccaatacaga
     1381 atttctgcaa aagggaatcg atctcggtgt tgcaaactcg attctgatca aggtcaacca
     1441 gattggcacg ctcacggaaa cactcgactg catcgaactc gcgcaaagaa acaaacgcac
     1501 agcgatcatc tcgcatcgat cgggcgagac tgaagatccg ttcatcgccg atctcgcagt
     1561 ggcgaccaac gcgggccaga tcaagaccgg ctccctctcg cgcacggatc gcatcgcaaa
     1621 gtacaaccag ttgctgcgga ttgaagaaga tctgcgtgac gcctcacgct atcccgggag
     1681 gaaagcattt catcagattg aggtgcggtt tgaataagaa acgcggtccg ctcgcgctga
     1741 tcataatcga cggttggggt tattcacctg ctcgcgacgg caatgccatc gcactcgcag
     1801 cactaccctt ctacgacgag ctctgcgaaa agtacccgca gacactgctg gaagcacatg
     1861 gactgcgcgt cggattgccg gccggcataa tgggaaactc ggaagtgggc catctgaaca
     1921 tcggttcggg ccgcgtgatt cgcatggacg tttcgttggt cgatcacgaa atcgaaacgg
     1981 gcgagttttt tcgtaacaac gtcttgacag gtgctatcga gcgtgcgcgc gatcgcggca
     2041 aagcactgca tttaatgggt ctctgctcgg atggccaggt tcattcgtcg ctgacgcatc
     2101 tctacgcttt gttgaagctg gcgagagatt tggaaatgca gcgggtctac atccactgct
     2161 ttctcgacgg gcgcgacacg ccgccgagtt cggctgtcaa ctatctctcc gcattggaaa
     2221 acaaagccgg cgagatcggt tgcggccaga tcgcgacggt gacaggccgc tactacgcga
     2281 tggatcgcga caagcgttgg gagcgcacca aacgtgctta cgatttgctg gtccatggca
     2341 tcggcgaacg aacaacggat ccggttaagg ccgccagaca gtcgtacgaa cgcggcgtga
     2401 cggatgagtt tgtcgagccg atctcgatcg tgcgtgcaac gatggaaccg gtagctacga
     2461 ttaaggacgg cgattcggta atctttttta acttccgtcc cgatcgcgcg cgacaactca
     2521 cacgcgcgct cacagtctcg aggttcaacg aattcgaagt ttccgagcgg cctaaagtgg
     2581 attttgtctg cttcacgatg tacgaccgca ctttcgatct gccgatcgcg tttcctccac
     2641 gcgagcatca caacgtgctc gcagaagttt ggagcaaagt gtgcgtgcgt aactatcgtc
     2701 tggccgaaac tgagaaatat gctcacgtta cttacttctt caacggcggc atcgagaagg
     2761 agtttgagtg cgagcggcgg ttgctggtcc cttcgccgaa gatcgccacc tatgatctcc
     2821 aacccgaaat gtcggctttc aaggttacag accaggtgtt acacggcatc gacgaaggcg
     2881 agacggacgt tttcgtcgtc aacttcgcca accccgacat ggtcggtcac accggcaaac
     2941 tcgataaaac aatcgaggcg tgccaatacg tcgacacctg tctcggctgg atcacgaaac
     3001 gaatgcgcga tgcgcgcggc acaacaatca tcaccgccga tcacggcaat gcggagcaga
     3061 tgatcgatc
//
LOCUS       DASTEY010000535         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_967779, whole genome shotgun sequence.
ACCESSION   DASTEY010000535 DASTEY010000000
VERSION     DASTEY010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967779"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2864)
                     /locus_tag="VFO72_09410"
     CDS             complement(<1..2864)
                     /locus_tag="VFO72_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9479552.1"
                     /translation="MQINGLEVVTSQSTQGVATFTLQFSLDKNIDAAATDVQTAISQA
                     TGSLPADLPSPPTYSKTNPNDQPLMYIAMTSDSVTPGQLYDYASTNVGQRLSILPGVS
                     QVAVYGTKSAIRIKANPAAMWARGISVDDLSNAIKNGTSYTGAGQLDGSSGTSILRPQ
                     GQLETAEQYGNLIVGGSNGAPIYLRDVATVKESVQDERVNMRFWVRGHPVPSATVIVA
                     VSRRAGANAVEVSKSIYETLPMINTELPGSIRITPIYDRSQNIVHSVADVQMTLVIAF
                     VLVVMVIFAFLGRVTDTLIPVVALPLSLLITFIVMRGLGYSLDNLSLMALTLAIGFLV
                     DDAIVFLENTVRRMEHGEQPFQAAINSAKEISFTIMSMTISLAAVFIPLVFMTGLVGR
                     IFREFAITIVVAILASGLVSLTLTPLMCARMLSARGVGSTKTWMERVIGGIEKRILAF
                     YGGSLWWFLRHRWVSPVIWTICLAGTIWLFILVPKTFLPPGDSGVIFGAFIAREGSSP
                     KQMREYQDRVDEMLRNDPNVVTDFTMTGATGFLASNQGITFTFIGPPEGRRPIAEVTA
                     EMMGKLNTIPGLMTFLRPFPVLEISTGVTSQQQGQYAFSVSGANPNQVYEVGQKLMAK
                     LMEYPGFLSVSSDYYNNTPNLNIDIRRDQAKTYGVSEARILSLLRTAYSQNYLYLIKK
                     PEDQYQVILEMDDSVREKPEDLSLLYIKSDDGRNLVPLRELVTWKSTLGPQVVNHLNQ
                     FTSMTLFFNLKPGVALGDATDYITQSAAQIVPQGVRAELQGEALTFSRTVTSLTILMA
                     LAVFVMYVILAILYESYVHPLTVLSTLPTALVGGLLTLIVFGEEASLYAFVGMFMLMG
                     IVKKNGIMIVDFARHRVEAGEAPDQAIHDASMDRFRPILMTTLAAVFGAIPIALGYGA
                     DGSSRRPLGLVVVGGLIVSQFITLYITPVIYLYLEQFQENV"
BASE COUNT          653 a          840 c          795 g          599 t
ORIGIN      
        1 acgttctcct gaaactgctc gagatacagg tagattaccg gcgtgatgta gagcgtgatg
       61 aactgcgaca caatgaggcc gccgaccacc accaaaccga gcggccgccg cgacgaacca
      121 tcagcaccgt aacccagcgc aattgggatc gcgccaaaca ccgccgccaa agtcgtcatc
      181 agaatcggcc ggaaacgatc catactcgca tcgtgaatcg cctggtccgg cgcttcaccg
      241 gcttcgacgc gatggcgcgc aaagtcgaca atcatgatgc cgttcttttt cacaatgccc
      301 atcagcatga acataccgac gaacgcgtag agcgacgcct cttcgccaaa cacgatcaac
      361 gtcaatagac cgccgaccaa cgccgtcggc aacgttgaaa gcacagtcag cggatgaacg
      421 tagctttcat acaggattgc aagaatcaca tacataacga acacggccag cgccatcagg
      481 atcgtcagac tcgtgacggt gcggctgaac gtcagtgctt cgccttgcag ttccgcgcgc
      541 acaccctgcg gcacgatttg agccgctgat tgcgtgatgt agtcggtcgc atcgcccagc
      601 gccacgcccg gtttcaagtt gaaaaagagc gtcatgctgg tgaactgatt gaggtgatta
      661 accacttgtg gtccaagagt cgatttccac gttacgagtt cgcgcaacgg cacgagattg
      721 cgcccgtcgt ctgacttgat gtagagcagc gacaggtctt ccggtttctc acgcaccgag
      781 tcgtccatct cgaggatgac ctggtattga tcctcgggct tcttgatcaa gtaaagatag
      841 ttttgcgaat acgcggtacg cagcaacgac agaatacgcg cttcagagac gccgtatgtc
      901 ttcgcctgat cgcgccggat atcgatgttg agattgggcg tgttgttgta atagtcggac
      961 gaaacactga ggaaccccgg atactccatc agtttggcca tcagcttctg gcccacctca
     1021 tagacctggt tcgggtttgc tccggaaaca ctgaaggcat actgtccctg ttgctggctg
     1081 gtgacgcccg tgctgatctc gagcacggga aacggacgca ggaacgtcat caagccggga
     1141 atggtgttga gcttgcccat catctcagcc gtgacttcag cgattggcct gcgtccttcc
     1201 ggcggtccga taaaagtaaa cgtgatgccc tgattggaag ccagaaatcc ggtggcgccg
     1261 gtcatcgtga aatcagtgac cacgtttgga tcgttgcgca acatctcgtc gacgcggtcc
     1321 tgatactcac gcatctgttt cggtgatgaa ccttccctcg cgatgaatgc tccgaaaatc
     1381 acaccgctgt cacctggcgg cagaaacgtt tttggcacga gtatgaacag ccagattgtt
     1441 cccgccagac agatggtcca gatcactggc gagacccaac gatggcgcaa aaaccaccag
     1501 agcgagccgc cgtagaaggc aaggatccgt ttctcaatgc caccgatgac gcgttccatc
     1561 cacgtctttg tcgaaccaac gccgcgcgcg ctcaacatcc gcgcacacat caaaggcgtc
     1621 agcgttaacg acaccaatcc tgacgcaagg attgcgacca cgatcgtgat cgcgaactcg
     1681 cgaaagattc gtcccaccag tcctgtcatg aagacgagcg ggatgaacac ggccgcgagt
     1741 gagatcgtca tcgacatgat cgtgaaactg atctctttcg cgctgtttat ggcggcttga
     1801 aacggctgtt cgccgtgctc catgcggcgt accgtgtttt cgagaaagac gatcgcgtcg
     1861 tcaacaagaa atccgatcgc cagcgtcagc gccatcagcg agaggttgtc gagactgtaa
     1921 cccagaccac gcatcacgat gaacgtgatt agcaacgaca acggcaacgc tactaccgga
     1981 atcaacgtgt ccgtgactct accgagaaag gcaaagatca ccatcaccac cagcacgaac
     2041 gcgatcacca gcgtcatctg cacgtccgca actgagtgaa cgatgttctg cgaacggtca
     2101 tagattggcg tgatgcggat cgagccgggc agttccgtgt tgatcatcgg caacgtttcg
     2161 tagatcgatt tcgaaacttc gacagcgttc gcgccggcgc gccggcttac agcaacgatc
     2221 acagtcgccg acggaaccgg atgcccgcgc acccagaatc gcatgttgac gcgctcgtcc
     2281 tgcaccgact cctttacggt cgcgacgtcg cgcaggtaaa ttggcgcgcc attcgagccg
     2341 ccgacaatca ggttgccata ctgctccgca gtttctagtt ggccctgggg cctgaggatc
     2401 gatgttccgg acgaaccgtc taactggccc gctcccgtgt aactcgtacc gttcttaatc
     2461 gcgttgctca ggtcatcgac cgaaatgccg cgcgcccaca tcgctgccgg attggccttg
     2521 atgcgaatcg ccgacttggt tccatagact gctacctgcg aaacacccgg caggatgctg
     2581 aggcgctggc ccacgttggt gctggcgtag tcgtacaatt gacctggcgt gaccgagtcg
     2641 ctggtcatcg cgatgtacat gagcggctga tcgttcgggt ttgtctttga gtaggtcggc
     2701 ggcgaaggaa gatcggccgg aagacttccc gttgcttgcg aaattgcggt ttggacgtcg
     2761 gtcgccgcag cgtcgatgtt cttgtcgagt gagaactgaa gcgtaaacgt tgcgacgcct
     2821 tgcgtactct gcgaagtcac gacttccagt ccgttgattt gcatgaactg gcgctcgagc
     2881 ggcgtgg
//
LOCUS       DASTEY010000536         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1560561, whole genome shotgun sequence.
ACCESSION   DASTEY010000536 DASTEY010000000
VERSION     DASTEY010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560561"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="menC"
                     /locus_tag="VFO72_09415"
     CDS             complement(<1..1074)
                     /gene="menC"
                     /locus_tag="VFO72_09415"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9479553.1"
                     /translation="MIIQSIELREICLPLLYFFETSFGRTTERRIVLARVRDADGAEG
                     WGECTAGEGPFYSDEWTDSVWSTLCEFLGPMVLGQEVESAADVFALMRPVRGHRMAKA
                     TIETACWDLEAKKLGVQLWKHLGGTRHEIASGVSIGIQDTPDILLEKIRIEVDAGYQR
                     IKIKIKPGWDTAIVALVRKEFPDIRLMGDANSAYTLADVSLFEELDRFNLMMLEQPLS
                     HDDIFDHATLQRQIQTPICLDESIRTAADATHAIALGSCKIINVKLGRVGGHAQAKRV
                     EQVAREHDIPIWCGGMLESGIGRAHNIAMATLAGFSLPGDVSASARYWEEDIIDPPVM
                     VSAKGTITAPDAPGIGFNLNLPRI"
     gene            1265..>3357
                     /locus_tag="VFO72_09420"
     CDS             1265..>3357
                     /locus_tag="VFO72_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9479554.1"
                     /translation="MRLICPVLSPRLLLILCGFVLSVLIISLITSPSSALTNNGSITT
                     LEAPLTENFDTLASTGTGIAWTDNSTIPGWYSSRVTYNSGTGSSNAGALYSFGVAATN
                     PVTDRALGSVGSGSTGTVFWGVRFVNNTGNTITSLDVNYNGEQWRTGGSSSATPSVAQ
                     TADFQYQVANAGVITAINTPSAGWLDHDALDFTSPIFGTAASALDGNAAANRTAKSST
                     ITLTVAPGQEVWLRWRDIDHANNDHGLAVDDLSVTAHGGGELNLSVGDATVTEGDSGT
                     MTATFTVTLSTPAEEDVNFDIATQDNTATTADNDYVAKSLTGQTISAGSTSYNFEVTV
                     NSDTTFEPNETFFVNVTNVTGANVNDAQGVGTITNDDCPASPGDVVISQVYGGGGNAG
                     AILTHDFIELFNQGSTTVDLTGWSVQYISASGTGTWQVTPLSGSIAPGGYYLVQEAAG
                     AGGTTPLPAPDATGAIAMAATAGKVALSSSTTPFSGASPSCPIDFVGYGGANAFEGAG
                     PTAATSNTTAALRKRGGCFDSDNNNIDFSIGSPNPRNTASPTNSCAPISLNIHDIQGN
                     GAATPFAGQFVSTSGVVTARKSNGFFLQNLPVNHDIDPATSEALFVFTSSAPAVSTGD
                     TVDVLGTASEFFNLTQVESTLPGDVTVTSSGNPLPVPVVLTTTILNPAGLPDQLERFE
                     GMRMHADTLVSVAPT"
BASE COUNT          740 a         1000 c          880 g          737 t
ORIGIN      
        1 gatgcgcggc aggttaagat tgaacccaat acctggcgcg tccggcgccg tgatcgttcc
       61 ttttgcactc accattaccg gcggatctat gatgtcttct tcccagtaac gcgcactcgc
      121 cgacacgtcg ccaggcaaac tgaaaccggc aagcgttgcc atcgcaatgt tgtgggcgcg
      181 tccaattccc gactccaaca tgccgccaca ccaaatcgga atatcgtgct cacgcgccac
      241 ttgctcgacg cgtttcgcct gggcgtgtcc gccaacgcgc ccgagtttga cgttgatgat
      301 cttgcacgat cccaacgcga ttgcgtgagt cgcgtctgcg gcagtgcgaa tcgattcgtc
      361 caggcagatc ggcgtctgaa tctgtctttg taacgtggcg tgatcgaaga tgtcgtcgtg
      421 cgacagtggc tgctcgagca tcatcaagtt gaaccggtcg agttcctcaa acagtgaaac
      481 gtcagccagt gtgtaagcag aatttgcgtc acccatcaaa cgaatgtcag gaaactcttt
      541 tcgcacgagc gcaacaatag ctgtgtccca cccgggtttg atcttgatct tgatccgctg
      601 gtaaccggcg tccacttcta tacgaatctt ttcgagaaga atatcaggtg tgtcttgaat
      661 accgatcgaa acgcctgacg cgatttcgtg gcgcgtgccg ccaaggtgtt tccacaattg
      721 cacaccaagt ttcttcgctt cgagatccca gcaagccgtt tcaatcgtcg ctttcgccat
      781 gcgatggcca cgcactggtc tcatcaacgc gaacacgtca gcagcacttt caacctcctg
      841 accgagtacc attggaccaa gaaattcaca cagcgttgac caaacgctat cggtccattc
      901 gtcgctgtag aacggtcctt caccggcagt gcactcgccc caaccttcag cgccgtcggc
      961 gtctcgaaca cgcgcgagca caatgcgccg ttcagtggtt cggccgaaac ttgtttcgaa
     1021 gaagtagagc agcggcaggc agatttcgcg cagctcaatc gattgaataa tcaaggggaa
     1081 agctgttcct ccgtgacggg cattatgcct gtccgtcgtg cagttgtcag cttgacccgc
     1141 ccctacagaa gctggtaaaa ggcacgcaca atttccctta ccggcctcaa gtacctccgg
     1201 tattcctctc aataatttaa cgtgcggagt ttgtctaatt ctggtttcta aaaggaagat
     1261 ctgtatgcgc ctgatttgcc ccgttctctc gcccagactg ctgttgattc tgtgcgggtt
     1321 tgtgttgtcc gtactcatca tttctcttat cacatcgcct tcttccgctc tgacaaataa
     1381 cggaagcatc accacgttag aggcacccct gactgaaaac tttgacacgc ttgcttcgac
     1441 tgggacgggc atcgcctgga ctgacaactc gaccataccg ggctggtact cgtcgagagt
     1501 cacttacaac tccggtacgg gatcgtccaa cgcaggcgca ctctacagtt ttggcgtggc
     1561 ggcaacgaat ccggttacgg atcgagcact gggttctgtc ggttcaggca gcacaggaac
     1621 cgttttttgg ggagtgaggt ttgtcaacaa caccggcaac acgatcactt cactcgatgt
     1681 taattacaac ggcgagcagt ggcgaacggg aggctcgagc tcggccaccc cttcagtcgc
     1741 tcagacggcg gactttcaat accaggtcgc gaacgcgggt gtcatcactg ctatcaacac
     1801 gccttccgcg ggctggctcg atcacgatgc acttgacttc accagtccga tcttcggcac
     1861 cgcggcgagt gcgctcgatg gcaacgccgc agcgaatcgt acagcgaaat cgtcaacgat
     1921 cacgttgact gttgctcccg gacaagaggt ctggctccgt tggcgcgaca ttgatcacgc
     1981 aaacaacgat cacggactcg ccgtcgacga tctttctgtc accgcacatg gtggtggcga
     2041 attaaatctg tcagtcggcg acgcgacggt aacggaaggc gatagcggca ctatgaccgc
     2101 aacgttcacc gtcacactgt ccacacccgc tgaggaagac gttaacttcg acatcgcgac
     2161 tcaagacaac accgcaacga ccgccgacaa cgactacgtt gcgaaatcgt taaccggcca
     2221 gacgataagt gccggcagca ccagttacaa cttcgaagta accgtcaaca gcgacaccac
     2281 gttcgagcca aacgaaacct tcttcgtcaa cgtgacgaac gttaccggcg caaacgtcaa
     2341 cgacgctcaa ggtgtcggaa cgatcaccaa tgacgactgt ccggcttcgc ctggtgacgt
     2401 tgtgatcagc caggtttacg gtggtggcgg caacgctggg gccatcctca cacacgattt
     2461 catcgaactc ttcaaccagg gttcgacaac ggtcgatctc acgggctggt cagtgcagta
     2521 tatttccgcc agcggtactg gcacgtggca agtaactcca ttgtcgggct cgattgctcc
     2581 aggtggttat taccttgttc aggaagccgc cggtgctggc ggaacaacac cactcccggc
     2641 accggacgca acgggcgcga tcgcgatggc ggcgaccgcg ggaaaggttg cgctctcgag
     2701 ttccactaca ccattcagtg gcgccagtcc atcgtgcccc atcgatttcg ttgggtatgg
     2761 tggagccaat gcgtttgaag gcgcgggtcc aactgctgca accagtaaca ctaccgcggc
     2821 gctgcgcaag cgtggcggat gcttcgattc ggataacaac aacatcgatt tttcaatcgg
     2881 cagtcccaat ccgcgcaaca ccgcatcgcc gacgaatagc tgcgcaccga tctcgctcaa
     2941 cattcacgac attcagggca acggcgccgc aacgcctttc gccggtcaat tcgtgagcac
     3001 gagtggagtt gtgaccgcac ggaagtccaa tggtttcttc ctccagaatc ttccggtgaa
     3061 ccacgacatc gatcccgcga cgtccgaagc gctgtttgtc tttacttcca gcgcgccggc
     3121 agtttcaacg ggagacacgg tcgatgttct gggaactgcg agtgagttct ttaacctgac
     3181 acaagttgaa agcacgttgc cgggcgacgt gactgtaacc tcttcgggaa atccgctacc
     3241 ggtgccagtt gtgctcacga ctacgattct caatccagcc ggtttgcccg atcagctcga
     3301 gcgcttcgag ggtatgcgca tgcatgcgga cacgctcgtt tcggtcgcgc cgacgaa
//
LOCUS       DASTEY010000537         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_836303, whole genome shotgun sequence.
ACCESSION   DASTEY010000537 DASTEY010000000
VERSION     DASTEY010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836303"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="VFO72_09425"
     CDS             <1..2214
                     /locus_tag="VFO72_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479555.1"
                     /translation="WKKQNTVFEDVAAFGSSTATLTGDGDPEQLMGTAVTPGYFEVVG
                     VQPALGRAFLPEEHDLGRDQVVILGHNLWQRRYGGDKNIIDQKITLDGNSWTVVGVMP
                     PGIYPARPSTAGRLEFDKKGEDFWRPMSYFVHFASLRTAHILGVVARMKPGVTIEQAV
                     AEMNTIGARLEQEHAANKGEGIIVNPFLNEVVGDVRPALITLLVAVGLVLLIACANIA
                     GLLLAQHAARTKEIAIRAALGAGRRRLVRQFFAEGLLLSLVGTVAGLAVAQLGLNVLL
                     KFVPQRIPRLDAIDLDWRVLGFTLLLSLITCLVFGLIPAWYASKPDLQTALEQSGRTS
                     GPGAARLRFRQLLVVFQVSVAVMLVVGAGLLVKSFWLLQRVDPGFKAERVVSAGLTLP
                     RTKYAKPTDINNFFNQLLERVSALPGVASATIAYDHPLQSNWVDSFEIEGSVAPADSQ
                     SQSANFIPVGPNYFETVRAQIVRGRPFTPQDDADHPGAAIVNEAFVRHYFPNEDPLGR
                     RLRPSPPGRIWENQRLTSFEVVGVVRDVKFDGLAAGSEPAYYLPASQAPLNDMTMLVR
                     TTSDPRTVIPALRQAVWSIDPNQPISNVNTLEQIVSDSIAQPRLNMLLMMLFGGLALL
                     LSAVGIYGLLSYAVTQRTQELGIRMALGANVADVLKLVLRQGMFLALIGEAIGLAGAF
                     ALTRLMRGLLFGVTPTDTTIFAAVVVVLSLTALLACYLPARRATKVDPLVALRYE"
     gene            2380..>3455
                     /locus_tag="VFO72_09430"
     CDS             2380..>3455
                     /locus_tag="VFO72_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479556.1"
                     /translation="MSTFWQDVRYGVRMLWKNRGISAIVILALALGIGANTAIFSVVN
                     TVLLRPLPYDESDRLVFFHEKSPVLDEMSISYPNFLDWRAQNQSFEKIGVYNRGSYNL
                     TGTGDAERIVTGQMSADIFTVLRVNPLHGRVFTNDEDQPGGTPVVVLSYGFWQRRFGG
                     QTSILNQPITLNGKSYTVIGVMPETFIGPSPGSSRMEMFVPVGQLSDQASWKSRGNHP
                     GLYGVGRLKPGVTIEQADADMNTIAANLEKQYPDTNAGTRIKLLPMLEIFVSDVRRAL
                     WVIFAAVGFVLLIACANIANLLLARATARRKEMAIRTAVGASRWRLARQLLTESVVLS
                     VIGGALGLLLARWGIDLILYVSPDA"
BASE COUNT          708 a          934 c         1044 g          769 t
ORIGIN      
        1 tggaaaaaac agaacaccgt cttcgaagac gtcgcggcgt ttggcagctc gacagcgacg
       61 ctcacgggag atggcgaccc cgaacaactg atgggcaccg ctgtcactcc agggtacttc
      121 gaggtggttg gtgttcagcc ggcacttggt cgtgcctttc tgccggaaga acacgatttg
      181 ggaagagatc aggtcgtgat tctgggtcac aatctgtggc agcgtcgcta cggtggcgat
      241 aagaacatca tcgaccaaaa aatcacactg gatggcaaca gttggactgt tgtcggagtg
      301 atgccgccag gcatttatcc ggcacggccc agcactgccg gacggctcga gtttgacaaa
      361 aaaggcgagg acttctggcg accgatgtcc tacttcgtcc attttgcttc gctgcgaact
      421 gctcacattc tcggcgtggt ggcaaggatg aagcccggcg tcacgatcga gcaggccgta
      481 gcggagatga acacgattgg cgcccgcctc gagcaggaac acgcggcaaa caagggcgag
      541 ggcatcatcg tcaatccgtt cctgaacgag gtcgtgggcg atgtgcgacc cgcgctcatt
      601 acgttgctgg ttgcggtcgg actagtgctg ttaattgcgt gtgccaatat cgccggactg
      661 ttgttggccc aacatgccgc gcgcaccaaa gagatcgcca tccgcgccgc gctgggcgca
      721 ggccggagac gattggtgcg gcagttcttc gccgaaggac tgctgctgtc gttggttggt
      781 accgttgccg gactcgcagt ggcgcaatta ggactgaacg tattgttgaa gtttgtgccg
      841 caacggatcc cacggttgga cgcgatagac ctggattggc gtgtgcttgg tttcacgctg
      901 ttgctgtcgc tgatcacgtg cctggtgttt ggcttgattc cggcgtggta tgcatcaaag
      961 cccgatttac aaaccgcgct cgagcagagt ggccgcacgt cagggccggg cgcggcgcgt
     1021 ttgcgttttc gacagttgtt ggtcgtattt caagttagtg tggcggtaat gctggtagtt
     1081 ggcgccggct tgctggtgaa gagcttctgg ttgctgcaaa gagtagatcc cggattcaaa
     1141 gcggaacgag tcgtatcggc cggactgacg ttaccgagga cgaagtatgc aaaaccaact
     1201 gacatcaata acttcttcaa tcaattgctc gaacgtgtct ctgcgttacc aggagttgcg
     1261 tcagccacca tcgcttacga ccatccgctg caatcgaact gggtagattc ttttgagatc
     1321 gaaggcagcg ttgcgccggc cgacagtcaa tcgcagtcag ccaacttcat tccggttgga
     1381 ccaaactatt ttgaaacggt cagagcccag atcgtgcgcg gacgcccgtt cacgccgcag
     1441 gacgacgcgg accatccggg tgccgcgatc gtcaatgaag catttgtcag gcactacttt
     1501 ccgaacgagg atccgctcgg gcgccggctg cggccttcgc ctcctgggag aatctgggaa
     1561 aaccagcggc tgacgtcttt tgaagtagtg ggcgtcgtgc gcgacgtcaa attcgacggc
     1621 ctcgctgccg ggtcggaacc ggcttattac ttacccgcat cgcaggctcc gctgaacgac
     1681 atgacgatgc tcgttcgcac cacgagcgat ccgcgaaccg tcattccggc actgcgccag
     1741 gctgtgtggt ccatcgatcc caaccaacca atctcgaacg tcaacacgct ggaacagatc
     1801 gtctctgaca gcatcgcgca gcctcgtctc aacatgctgc tgatgatgct gttcggtgga
     1861 ctcgcattgc tgctgtcggc cgttggcatt tatggcctgt tgtcttacgc cgtaacacaa
     1921 cgcacgcagg agctgggaat ccgcatggcg ctcggcgcga atgtcgcaga cgttttgaag
     1981 ctcgtgctca ggcagggaat gttcctggcg ttgattggcg aggcgattgg tctggcagga
     2041 gcttttgctc tcacgcgttt gatgcgtggg ttgttgtttg gtgtgacgcc gactgatacc
     2101 acgatctttg cggccgtcgt ggtcgtgtta agtctcactg cgttgctggc gtgttatctg
     2161 ccggcgcgac gggcgacgaa agtggatccg ttagtagcgc taaggtacga atgaggtcat
     2221 tgccgatttt cgattgccga ttgccgattg gcaactgcca actgacgcgc tctacatcgc
     2281 aaggcgaaat tcgagaatcg ttgcaatcgg caatcggcaa tcggcaatcg tcaatgacca
     2341 aattggcaat gaccaaattg gcaataagga gatctaagca tgtcgacatt ctggcaagac
     2401 gtccgttatg gcgtgcgaat gctctggaaa aatcgtggca tctcggcaat cgtgatcctg
     2461 gcgcttgcac tcggtatcgg cgcgaatacc gcgatcttca gcgttgtgaa cacagtgctg
     2521 ttgcggccat tgccttacga cgaatcagat cgcctcgtgt ttttccacga gaagagcccg
     2581 gtgctcgatg aaatgtcgat ctcctacccg aacttcctcg actggcgcgc acagaaccaa
     2641 tcgttcgaaa agataggtgt ctacaatcgc ggcagctata acctgaccgg cacgggcgac
     2701 gctgaacgga tcgttacggg ccagatgtcg gcagacatat tcacggtgtt gcgcgtaaat
     2761 cctttgcatg gacgtgtgtt taccaacgac gaagaccagc ccggcggcac gccggtggtc
     2821 gtgttgagct acggtttctg gcagcgccgg tttggcggtc aaaccagtat cttaaaccag
     2881 ccgatcacgc tcaacggcaa gagctataca gtcatcggcg tgatgccgga gacgtttatt
     2941 ggtccaagcc ccggttcgag ccgcatggag atgtttgtgc cggtgggcca actttcggac
     3001 caggcgagct ggaaaagccg cggcaatcat ccgggtctgt acggcgttgg ccgtctcaaa
     3061 ccaggtgtca cgatcgagca ggcagatgcg gacatgaaca caatcgccgc gaatctcgag
     3121 aagcagtatc ccgacacgaa cgccggcacc cgcataaaac tgctacccat gctcgaaatc
     3181 ttcgtcagcg acgtccgacg cgcgctttgg gtgatcttcg cagccgtcgg attcgtgctg
     3241 ttgattgcct gcgcgaacat tgcaaacctg ctgctcgccc gtgcgactgc acgccggaaa
     3301 gagatggcga tccgcacggc tgtcggcgcg agtcgctggc gtctcgcgag gcaattgctg
     3361 acggaaagcg ttgtgctttc ggtgattggc ggtgcgttgg gtctgttact agcaaggtgg
     3421 ggcatcgatc tgattctgta cgtcagtccg gatgc
//
LOCUS       DASTEY010000538         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1461959, whole genome shotgun sequence.
ACCESSION   DASTEY010000538 DASTEY010000000
VERSION     DASTEY010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461959"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFO72_09435"
     CDS             complement(<1..1178)
                     /locus_tag="VFO72_09435"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479557.1"
                     /translation="MKKKNLLHRVIIIVIVTIVGLYLVIGPRRRPTLKDFTWSGIKAS
                     LASNIKLGLDLQGGSHLVMRVKTEEFLRRLTEENYIAAQNAAKDAGYDIKGGQAQTSG
                     SYRIVLEPGDPSKASEIKDAIEKKVELGDTSVWAYDASGAQMTWTMTGAAERLLADNA
                     TTQALNIIDSRINALGITEPTLQTHGAQSSHQILLQMPGVQDPERVKEILRGESRLEL
                     VHVIGPPSPAPATTYPTEADAIASLNTNGVVPPNRKVLPYSERMEPTANQDPNQQRPK
                     QWVVVEVPAIIDGSELRNATAIPSQGGRADDYEINFSLKKSGADKFGAWTGAHINEYM
                     GVVLNGEVKSIAYIKSQIFDQGQITGRFTQQSAEDLALTLRSGALPAPIEYLEERTVG
                     P"
     gene            complement(1196..1504)
                     /gene="yajC"
                     /locus_tag="VFO72_09440"
     CDS             complement(1196..1504)
                     /gene="yajC"
                     /locus_tag="VFO72_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET9479558.1"
                     /translation="MHTFLIFQSGAGGLIQLMPILLIILVFYFLIIRPQQKRQRQLQE
                     TIANLKIGDRIVTTGGVIGVITTVRDTSFLIRSADKSILEIARSAVAGIDEEGRQAST
                     "
     gene            complement(1545..2711)
                     /gene="tgt"
                     /locus_tag="VFO72_09445"
     CDS             complement(1545..2711)
                     /gene="tgt"
                     /locus_tag="VFO72_09445"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9479559.1"
                     /translation="MRFKVDAQDGEARAGVLTTRRGEIETPVFMPVGTAGTVKGIRFE
                     ELESPDLDARIILGNTYHLWLRPGIDVIKAAGGLHRFIGWNRALLTDSGGFQVWSLGA
                     LRKITEEGTEFRSHVDGSLKFLSPEVSMEVQAALGSDIVMVLDECAPGKATHEETERS
                     MELTARWAKRSKAAFDRLQSEGADTGRIETSSELSGRQALFGIVQGAAHLDLRRRSLE
                     QTVASGFDGYAIGGLSVGEEKPVMFEVIDDIAPRMPREFPRYLMGVGTPEDLVEAVAR
                     GVDMFDCVLPTRNGRTGQAFTSRGKLNIKNAQWAKDPRPLDESCHCSVCRRHSRSYLR
                     HLYLTGEMLASILLTHHNLAFFLDTMRRVRQSIRSGVFLKFRREFTERLDANGE"
BASE COUNT          560 a          787 c          771 g          651 t
ORIGIN      
        1 ggtccaaccg tacgttcttc gagatactcg atcggcgccg gcagtgcgcc cgagcgcaag
       61 gtcagcgcga gatcttcggc tgactgctgc gtaaagcgtc cggtgatctg cccctgatcg
      121 aagatctgcg acttgatgta ggcgatcgac ttcacttcac cgttgaggac cacacccatg
      181 tactcgttga tgtgggcgcc agtccacgcg ccaaacttgt ccgcgcctga ttttttgagc
      241 gagaaattga tctcgtaatc gtctgcgcgt ccaccctggc tcgggatcgc agtcgcgttt
      301 ctcaactcgc tgccgtcgat gattgcgggg acctcgacca cgacccattg ttttggtctc
      361 tgctggttcg gatcctgatt cgccgtcggt tccatccgtt cgctgtaagg taaaaccttg
      421 cgattcggag gaacaactcc attggtgttg agcgacgcaa tggcatcggc ttcggtgggg
      481 taggtcgtcg cgggcgcggg actgggcgga ccgatcacgt ggaccagttc gagtcgcgat
      541 tcgcccctaa gaatctcttt gacacgctcg ggatcctgca cgcccggcat ctgcagcaga
      601 atctgatgcg aactctgggc gccgtgagtt tggagcgtgg gttccgtaat gccgagagca
      661 ttgatgcggc tgtcgatgat gtttagtgcc tgcgtggtgg cgttatccgc aagcaaccgc
      721 tcggcggccc ccgtcatggt ccacgtcatc tgcgcgccgg acgcgtcgta agcccaaact
      781 gaggtgtcgc cgagttctac tttcttttcg atcgcgtcct ttatttcgct ggccttggac
      841 gggtctcccg gttctagaac gatccgatag ctgccgctcg tttgcgcctg accacccttg
      901 atgtcatatc cggcgtcttt ggcggcgttt tgcgcggcaa tgtagttttc ttccgtcagt
      961 cggcgcagaa actcttctgt tttgactcgc atcaccagat gcgagccacc ctggaggtca
     1021 agaccgagct tgatattcga agccagcgag gctttgatgc ccgaccaggt aaagtccttt
     1081 aacgtcggac gccggcgcgg acctataaca aggtaaaggc cgacaatcgt gactatgaca
     1141 atgattatga cgcgatgaag gaggttcttt ttcttcatta ccgaatcgtg tggaattaag
     1201 tacttgcctg tcttccttcc tcgtcgatgc cggcaactgc cgaacgggca atttccaaaa
     1261 tcgacttatc ggcgctgcga atcagaaaac tcgtgtcacg cacggtagtg ataacgccga
     1321 taacgccgcc agtagtgacg atgcgatctc caatcttcaa gttggctatg gtttcctgaa
     1381 gttgccgctg ccgcttctgt tgaggcctta ttataaggaa gtagaagacc aggatgatga
     1441 gcagaatggg catcaactgt atcaacccac cggctccact ctggaaaatc aaaaaagtat
     1501 gcattagttc ttaaacaaac gaaggcggcc aggcgcgcga actactattc accattcgca
     1561 tccagccgct cggtgaactc tcgccggaat ttaaggaaaa cgccagaccg gatagattgc
     1621 ctcacgcgtc gcatagtgtc aaggaagaac gccagattgt ggtgtgtcaa caaaatgctg
     1681 gccagcatct caccagtcag ataaaggtgt cgcagatatg aacgcgagtg gcgccggcaa
     1741 acggaacagt ggcacgactc gtcgagtggc cgcgggtctt tcgcccattg cgcgttcttg
     1801 atgttcagtt tgccgcgcga tgtgaaagcc tggccggtgc gtccgtttct tgttggcagg
     1861 acacagtcga acatatcgac accacgggcg actgcttcga cgagatcttc cggagtgccc
     1921 acgcccatca ggtagcgcgg aaattcgcgc ggcatacgag gcgcgatgtc gtcgattacc
     1981 tcaaacatga ccggcttctc ttcacctaca ctgagaccgc cgatcgcgta tccgtcgaaa
     2041 ccgctcgcga cagtttgttc gagactgcgg cgtctgagat ccagatgagc ggcgccttga
     2101 acgatgccaa acaatgcctg gcgcccgctg agttcgctgc tcgtttcgat acgtcctgtg
     2161 tctgcgcctt cagattgaag tcgatcaaag gcggctttgg aacgtttcgc ccagcgggcg
     2221 gtgagttcca tgcttcgttc ggtctcttcg tgcgtggctt tgccgggagc acattcgtca
     2281 agaaccatca cgatgtcaga tccgagcgct gcctgcactt ccatcgagac ttccggtgac
     2341 agaaacttca acgagccgtc gacgtgcgag cgaaactccg tgccttcttc ggtgatcttg
     2401 cgcagcgctc ccaggctcca cacctgaaag ccgcccgaat ccgtgagtag cgcgcggttc
     2461 cagccgataa agcggtgtag accaccggcg gctttgatca cgtcgattcc gggacgcagc
     2521 cagaggtgat aggtgttgcc gagaatgatc cgtgcatcga ggtcgggcga ttccaattct
     2581 tcaaaacgaa tgcctttgac agttccggcc gtgccaaccg gcataaacac aggcgtctca
     2641 atctcgccac gccgcgtggt gagcacaccc gcccgggcct ctccgtcttg agcgtcaacc
     2701 ttaaagcgca acgctggttc tgtaatctgt gtaatctgtg gttacctctt ggcctttttg
     2761 tgtttctca
//
LOCUS       DASTEY010000539         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_918476, whole genome shotgun sequence.
ACCESSION   DASTEY010000539 DASTEY010000000
VERSION     DASTEY010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918476"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFO72_09450"
     CDS             <1..530
                     /locus_tag="VFO72_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9479560.1"
                     /translation="EILENDARLLRILTPVMKLTTGKQSVMVASEVLETFGGAGYVED
                     TGLPVLLRDSQVLPIWEGTTNVLALDTLRALRGSDNGLSPLESAITRCLEIVQDERLA
                     DAARVVRSALDHAASWLAYAEQAGPEVLEAGARRFALTLGRTMELALLIKYAQLSHDC
                     AAARRFASSGIDLIF"
     gene            690..2492
                     /locus_tag="VFO72_09455"
                     /pseudo
     CDS             690..2492
                     /locus_tag="VFO72_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
BASE COUNT          634 a          690 c          719 g          568 t
ORIGIN      
        1 ccgaaatttt ggaaaacgat gcacgattgc tacgaattct tactccggtg atgaaactca
       61 ccactggaaa gcagtctgtg atggtagcaa gtgaggttct cgaaaccttt ggcggcgctg
      121 gttacgtaga agacaccggc cttccggttc ttctgcgcga cagtcaggtg cttccgattt
      181 gggaaggaac gacgaacgtg ctggcgctcg acacgttgcg cgccctgcgc ggcagcgata
      241 acggtttgtc gccgctggag tctgcgataa cgagatgtct cgagattgtt caggatgagc
      301 gtcttgcgga cgccgcacgc gttgtgcgct cggcgctgga tcacgcggcc agttggttgg
      361 cgtacgcgga acaagcaggg ccggaagtgc tggaggctgg agcacgccgt ttcgccctca
      421 ctttaggccg tactatggaa ttggcgttgc tgatcaagta tgcgcaattg tcacatgatt
      481 gcgctgccgc acgacgcttt gccagttccg gtatcgatct cattttttag ccacggatta
      541 cacggataga aacggatagg aggattctca tgaaccgcga caaacgcatt tcactattaa
      601 tcctgacgct gcttattgct gcctcaactg cgttgagcca gcacacgaag catgcgcagc
      661 ccgtcaagga gtacaccatc gagcagttca tgaacactgt tcgcatcggc ggcagctcct
      721 tctcgtcaga cgaaaagtcg atcctgtttc acaacaacaa gacaggcatc ttcaatgtct
      781 actcggtgcc ggcgacgggc ggcgccgcaa cccagttgac caactcagcc aaagaaagca
      841 cgttcgcggt ttcttatttt ccgtccgacg cgcgtttcat ctacacgtac gatcgaggcg
      901 gtaacgagaa caaccatctt tacctgcgcg agctcgatgg caccgaacgc gatctcacgc
      961 ccggcgagaa ggtgaaggca aacttcctcg gctggagtcg cgatcgtaaa tcgttcttct
     1021 tttccacgaa cgaacgcgac ccgcgcttct tcgatattta tgaaatgccg atcgacagtt
     1081 tcaaaccgtc gctcgtctac aaagacgaaa ccggctatgg attcggagcc atttccgacg
     1141 acaagaagta catcgcgctc gagaagagtg gcggctcgca ggccgactcc gatatttacc
     1201 tttacaatgc tgcgactaaa gagttgaaaa acatcacctc gcataccggc gacatggcga
     1261 ataacgcgca gacgttcgat ctcagctcca agtatcttta ttacctgact gactcaggca
     1321 gcgagtttag ctacgttgcg cggtacgatc ttgcgactgg caaaagcgaa gtcgtcgaga
     1381 aggcgccctg ggacgttttc tatacgtact tttctcaaaa cggaaagtac cgtgtgacgg
     1441 caatcaacga agatgcgcgc acgcgaatca agatctacga aacagccacg ggcaagctcg
     1501 tctccttacc gcaacttccg aatgccgaca tcaccggcgt gaatatctcg cggagcgaga
     1561 agctgatggc gttttatttg aacggtgatc gcagcccgag caatctttac gtttacgact
     1621 tcagcacgaa aaaggcgacg aagttaacgg aaagtctgaa tcctgagatc aatcccgccg
     1681 acctcgtcga ttcgcaggtg atacggtaca agtcgttcga cggcgtccag attccttcaa
     1741 ttctttacaa accgaagcag gcgagcgcgc agaacaaagt gccggcgatc gtgctggtgc
     1801 atggtggtcc gggagggcag acgcgtgttg gctacagcgc cctgacgcag tatctcgtca
     1861 atcatggtta cgtgatcctg ggagtcaaca atcgcggctc gagcggttac ggcaaatcgt
     1921 tcttcacgtc tgatgatggc aagcatggac gtgaaccgtt gtgggactgt gttgaggcga
     1981 agaagtatct tgtgtcgctc ggctacgtcg atgagaagta gatcgggatt atgggcggct
     2041 cttacggcgg ttacatgacg cttgctgcgc ttgcgttcaa gccgcaagag ttcgcggtgg
     2101 gtgtggacct gtttggagtt gcaaactggg tgcggacgct gaaatcgatt ccgccgtact
     2161 gggagtcatt ccgcaagtcg ttccacaggg agatcggcga tcccgaaaag gatctcgaaa
     2221 acctgaaggc gatctcaccg ctctttcatg ccgacaagat cgtgaagccg ctgatcgtgt
     2281 tgcaaggcgc aaacgatccg cgcgtcatca aacccgagtc ggatgagata gtggagaaca
     2341 tcaagaagcg taacggcgtg gttgaatacg tgttgttcga caacgaaggc cacggcttca
     2401 cgaagaaagc gaacgagatc aaagcctaca aagcaatcct cgacttcctc gatcgctacc
     2461 tgaagaacgg cgacgccatg agcagcagat aaaatttagc cacggattta cgcggattga
     2521 gatccggatt aatccggatc tatccgtttt atccgtggcc caaaaacgca aaggcgcaaa
     2581 gaccactccg caagcgatga tcttcgcgcc t
//
LOCUS       DASTEY010000540         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_390991, whole genome shotgun sequence.
ACCESSION   DASTEY010000540 DASTEY010000000
VERSION     DASTEY010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390991"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1579)
                     /locus_tag="VFO72_09460"
     CDS             complement(47..1579)
                     /locus_tag="VFO72_09460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9479561.1"
                     /translation="MGNISPLYQLDAFDWIVLIIYFAILTALAVYGSYRIKQVIDFWR
                     YRRFVPTPLARFAEADLPRITVQLPLFNELYVVDRLVKAVTAIDYPREKLEIQVLDDS
                     TDETAKVAEAVVAKYAAQGFDIQYIHRSDRTGFKAGALENGNRYAKGDLFAIFDADFV
                     PKPDCLRKLVDFFTDPMVGCAQMRWSHINGGYNLLTRLQTIMLDGHFVVEQTTRNRTG
                     GFFNFNGTAGIWRRQAIEMSGGWQHDTLTEDTDLSFRAQLMGWKFVYLLDEEAPAEIP
                     VEINAFKAQQRRWAKGVMQVGLKLYPRIWLAPLPLRVKLEMFFRLTGNISYPLMIVAS
                     FLQFPLLLVRYNQPFYHLMVLDLPLLFFSSISVVLFYGSAVWYLDKNRTPRLLHLPLV
                     MGLGIGLAFSNARAVLEALLGVKSEFVRTPKYRVEEATDATWKRKKYKRKRGLLPLLE
                     LAFAVYFLLAITYSARLHMWGTIPFLLLFFFGFGYMGVMSVLQTASGKKLAALLWRPL
                     KA"
     gene            complement(1749..>2453)
                     /locus_tag="VFO72_09465"
     CDS             complement(1749..>2453)
                     /locus_tag="VFO72_09465"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HET9479562.1"
                     /translation="NRGTFALSTRCILKAGQTRLFDFEPRIVTHDTNQMFTAGSYAPQ
                     VVPTRRPLFFWAISTAVVASLVALIIVAPLAAAGGHGHVATGIYRAFATLCHQLPERS
                     YFIDGHKLAVCSRCTGLYAGFALTLLVYPLIRSLRTTAIPARKWLVLAAIPTAIDFSL
                     TFFGIGENTHTSRLLTGLLLGSTAVFYVMPGIADLSLRALRNRPAEQPRVTFTLVSPD
                     SIASAPSDYSAPERRI"
BASE COUNT          583 a          651 c          721 g          498 t
ORIGIN      
        1 aagactaaca agactcactt ttttatcctg acattctgta atcctgtcaa gcttttagcg
       61 gccgccatag tagtgccgcc aacttcttac cgctggcggt ttgcagcaca ctcatcacac
      121 ccatgtagcc aaacccgaag aagaacaaca gcagaaacgg aatcgtgccc cacatgtgca
      181 ggcgcgctga ataagtgatg gccagcagga agtagacggc aaaagccagt tccagtaacg
      241 gcaacagtcc gcgtttacgc ttgtacttct tccgtttcca cgtcgcgtca gtagcttctt
      301 caactcgata cttcggcgtt cgtacaaact ccgacttcac accgagcaat gcttcgagga
      361 ccgcgcgcgc gttggaaaag gcgagtccga tgccgagtcc catcacaagc ggcaaatgca
      421 gcaagcgcgg tgtgcgattc ttatcgagat accagactgc cgatccgtaa aacaacacga
      481 ccgagatcga agagaagaac aacagcggca gatccagcac catcaagtga taaaacggct
      541 ggttgtagcg gacgagcagc agcggaaatt gcaggaagct cgccacgatc atcagcggat
      601 aactgatgtt tccggtcaaa cggaaaaaca tctcgagctt gacgcgcaag ggcagcggcg
      661 cgagccagat gcgcggataa agtttcaacc cgacctgcat cacgcctttg gcccaacggc
      721 gctgttgcgc cttgaacgcg ttgatctcga caggaatttc ggctggcgct tcttcgtcga
      781 gcagatagac gaacttccaa cccatcaact gcgcgcggaa gctgagatca gtgtcctcgg
      841 tcagcgtgtc gtgttgccag ccgccactca tctcgatggc ctgacgtcgc cagatgcccg
      901 ccgttccatt gaaattgaag aagccgccag tgcgattgcg cgtggtctgt tcgacgacga
      961 agtgaccatc gagcatgatc gtttgcaggc gtgtcagtaa gttgtaacca ccgttgatat
     1021 gcgaccagcg catctgcgcg caaccaacca tcggatcggt aaagaagtcg accagtttgc
     1081 gcaggcagtc gggtttggga acgaagtcgg cgtcgaagat ggcgaacagg tcgcctttgg
     1141 cgtatctgtt tccgttctcg agtgcgcccg ctttaaagcc ggtgcgatca gagcgatgga
     1201 tgtactgaat gtcaaaaccc tgggccgcgt acttcgcgac tacggcttcc gcgacttttg
     1261 cggtttcgtc ggttgagtcg tcgaggacct ggatctcgag tttctcgcgc gggtagtcga
     1321 tggccgtcac ggctttgacc aaacgatcga ctacgtagag ctcgttgaag agcggcaact
     1381 gtacggtgat gcgaggcaga tcggcttctg caaatcgggc cagcggcgta ggaacaaatc
     1441 ggcggtagcg ccagaagtca attacctgct tgatgcgata cgagccatag accgcgagtg
     1501 cggtgagaat ggcgaaatag atgatcagaa cgatccagtc gaaagcgtcc agttggtaga
     1561 gcgggctgat gtttcccaag gttttgttga caccgtcgtg gaagtatcgt tcgaagattt
     1621 cctgcggtgc gggacctatg ggagtgggcc agggagttgg tgccggagct tgcaacataa
     1681 aagcgccaga ttgtagctca gtaagtctcc tgacgacaaa gatggctcgg agttttgtgt
     1741 atttagggct aaatgcggcg ttcgggggcg ctgtaatcgc tcggggcgga ggcgatgctg
     1801 tcgggtgaca ccagcgtaaa tgtcacgcgc ggttgctcgg ccgggcggtt tcgcaacgcc
     1861 cgaagactta ggtctgcaat tcccggcatc acgtaaaaga cggcggtact tccgagtagc
     1921 agacccgtca gcaggcgtga agtatgcgtg ttttccccaa ttccaaaaaa cgtcagactg
     1981 aaatcgatgg cggtgggaat cgcggccaac accaaccact tgcgagcggg gattgcggtg
     2041 gttctgagcg aacgaatcag cggatagacc agcagtgtaa gtgcgaagcc ggcatagagt
     2101 cccgtgcaac gcgaacagac ggcaagcttg tgtccgtcga tgaagtacga acgctccggg
     2161 agttgatgac agagggttgc aaacgcgcgg tagatccccg ttgcgacgtg accgtgtccg
     2221 ccggcagccg ctaacggtgc gacgataatc agcgccacca gtgaagcgac caccgcagtg
     2281 ctgatggccc aaaagaacag cggccgtcgc gtcggaacta cctgaggcgc ataactaccg
     2341 gccgtaaaca tctgattcgt gtcgtgcgtg actatccgtg gctcaaaatc gaataaccgt
     2401 gtctggcccg ccttcaatat gcagcgtgtc gacaaagcga atgtgccgcg att
//
LOCUS       DASTEY010000541         5391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_391032, whole genome shotgun sequence.
ACCESSION   DASTEY010000541 DASTEY010000000
VERSION     DASTEY010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391032"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFO72_09470"
     CDS             <1..598
                     /locus_tag="VFO72_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9479563.1"
                     /translation="DELKKYFESNPDKFRKPEQVKLSEIYLVTTGKDEAAVRARALEL
                     ISQVRAGADFGALAAANSEREKGGQRTAPQDKGYVGEFDVPSLRDDLVAALKNVPAGG
                     VTEPIRTPEGYQILRVDARTPAGTTPTFQENRVREAMLMERQPKEREAYLQTLRNEAF
                     IKVTEAYSAGVEPLLKLNQPATAKSADKTEDKKNNKKP"
     gene            615..1682
                     /gene="dusB"
                     /locus_tag="VFO72_09475"
     CDS             615..1682
                     /gene="dusB"
                     /locus_tag="VFO72_09475"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET9479564.1"
                     /translation="MFVKPFKIRDVAIDPPLILSPMAGVTDVSFRRLIKRRGGVGLSV
                     SEFISVEGLTRSNPKSKRQMRFYEDERPFAVQIFGGQPERMAMAAEMAQEIGADILDV
                     NCGCPAPKVVKHGGGSGLLKDHARLESVLKAVKKVITIPLTVKIRAGFYDHTINAVET
                     AKLAEDCGVEHIALHGRTKEQGYRGLADWDLVRQVKEVVNVPVSGSGDVTTIEGAFAK
                     FRETGCDGVLIGRGAMANPWIFRQIEDAMHGRKIFEPTLAGKRAILHEYFDMLREDMP
                     ETPAINRMKQLAGQFTRGLQGGALFRTSIYHSHSVEEVLDRIEEYFSAIEAGRPYYGE
                     AGAPESRDHTEITDCAAFISV"
     gene            complement(1669..3000)
                     /locus_tag="VFO72_09480"
     CDS             complement(1669..3000)
                     /locus_tag="VFO72_09480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9479565.1"
                     /translation="MDRLKVWPTERENLFMTMMPRLLLFSLIATALAVGSACTAPTNN
                     SNAPVTQATPAPSPAASPASTDQASVQVTLPLLDALLADDAFVSELKNKANLSDDRIA
                     SLKRASQLEVDRLREANAEDAEEINGNGTDAPTRAAEQLRSILGDEKATQVASLANEF
                     WAKGDPEAEGANGAAFEMLKGPNAVPADTRVVVNIPAFRMDLFQNGSLVKSYKIGIGY
                     PEFPLPLGLRKAQTVIFNPPWTPPHSPWVAKMKNVTPGETVQPGSKDNPLGIIKIPIG
                     MPSLIHGGKAPAKIGKFASHGCVGMTNAQVKDFATQLIRAANNEVSDKEITQYLADKT
                     QTKTLKLDQVIPVELRYETIVLEDGKLYIYKDVYAQDTNTEENLRRVLEMHGARLEDL
                     SEDQRRQALEALQKPVKTEVVIDVSTLAGKGYPAPVNLDDGKPQTTRTKRR"
     gene            complement(3169..3732)
                     /locus_tag="VFO72_09485"
     CDS             complement(3169..3732)
                     /locus_tag="VFO72_09485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9479566.1"
                     /translation="MFLLAFIALVWLGTGRVPPPSLVESVPSVVQPVQTVAQPVSQIA
                     RIEPLIIPVAGVRPDQLTDTFSDSRSEGRTHDAIDIMAPAETPVLAAADGQIQRLFQS
                     ERGGTTIYQLSADQKLIFYYAHLARYADGLTEGKQVRQGETIAYVGDSGNAGIGNYHL
                     HFSIAIVSDPKRYWEGTNINPYLLLRH"
     gene            complement(3778..5010)
                     /locus_tag="VFO72_09490"
     CDS             complement(3778..5010)
                     /locus_tag="VFO72_09490"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9479567.1"
                     /translation="MAQPRIVVVGGGFAGLFTALELSGSHDVTLVTDSDHFVFTPMLY
                     EYFSGEVEAWHIGPRYDELLHKDVKLVKSPVATVNFDAQTVTPEQAEALKYDVLVLAV
                     GGVTNYAGVPGAEEFSLPFRKIEHADTLRRMMVDALDRIPPEMPPQDVRRELTFALVG
                     AGASGCELSTKMADLLIDAFKRRALHGEPRVMLLEMGDRVVPGMGQQIRELVEEALRE
                     SHVEVHTLTRVINVTREGVTFEHEGKQEQVKTAAVVWLGGVRMNPLVERLDVEKNNRG
                     LLLIRPTLQLSKHDNVFALGDIAFYPDAEPTLHGTAQLAFQEAGLAGHNVKAFLEGKE
                     LRTKHFEELGEAISLGTERAAVLTAGKAFGGALARQARFALYTSRLPTWHHRLRVGAS
                     WFFEGTAPRPLLPLGLER"
BASE COUNT         1127 a         1467 c         1612 g         1185 t
ORIGIN      
        1 tgacgagctc aaaaaatact ttgagtcgaa tcccgacaag tttcgtaaac ccgagcaggt
       61 gaagctgtct gagatctatc tcgttacgac cggtaaagac gaagcggctg ttagggcgag
      121 ggctttggaa ttgatctcgc aggttcgcgc gggcgctgac ttcggcgcac tcgcggccgc
      181 caactccgaa cgcgaaaagg gtggccagcg caccgcgccg caggacaagg gctacgtcgg
      241 tgaatttgac gttccgagtc tgcgcgacga tttggtggcg gcgctaaaga atgtgccggc
      301 tggcggcgtg actgagccga tccgcacccc cgagggttat cagattcttc gcgtggacgc
      361 gcgcacgcct gccggaacta ctccgacctt ccaggagaac cgcgttcgcg aggcgatgtt
      421 gatggagcgt cagccgaaag agcgcgaggc ctatttgcag actctgcgta acgaagcatt
      481 catcaaggtt acggaagctt acagtgcggg cgtcgaaccg ctgctgaaac tcaaccaacc
      541 ggcaacagcc aagagcgccg acaagacgga agacaagaag aacaacaaga aaccgtaacg
      601 ctccgtctgt atccgtgttc gtaaagccat tcaagatccg tgacgtcgcg atcgacccgc
      661 cgctaatctt gtcgccgatg gcgggcgtga cggacgtctc ctttcgtcgg ttgattaagc
      721 gccgcggcgg cgtcggactg tcggtctcgg agttcatttc ggtcgagggg ctgacgcgca
      781 gcaacccgaa gtcgaaacgg cagatgcggt tctacgaaga cgagcggccg tttgcggtgc
      841 agatcttcgg tggacagccg gaacgaatgg ccatggccgc ggagatggcg caggagattg
      901 gcgccgacat tctcgacgtt aactgcggct gtcccgcgcc gaaggtcgtg aaacacggcg
      961 gtggttccgg tctgctcaaa gaccacgcgc ggctggaatc ggttctcaag gcagtcaaga
     1021 aagtgatcac gattccgctg acggtgaaga ttcgcgccgg tttctacgac cacacgatca
     1081 atgcagtcga gaccgcgaag ttggcggaag actgtggcgt cgagcacatc gctttgcacg
     1141 gccgtaccaa agagcagggc tatcgcggtc ttgcagattg ggacttggtg cgacaggtga
     1201 aggaagtcgt gaatgtgccc gtgtccggca gtggcgacgt cactacgatt gaaggcgcct
     1261 ttgcgaagtt tcgtgagact ggttgtgacg gcgtgctgat cgggcggggt gcgatggcga
     1321 atccgtggat ttttcggcag attgaagacg cgatgcacgg gcgcaagatc ttcgagccaa
     1381 cacttgccgg caagcgcgcg atcctgcacg agtatttcga tatgttgcgt gaggacatgc
     1441 ctgagacgcc cgcgattaat cgcatgaaac agttagccgg gcagtttacg cgcggtctcc
     1501 agggaggcgc actgtttcgc acctcgatct accattcgca ttctgtcgaa gaagtgctcg
     1561 atcgaatcga agagtacttc agcgcgatcg aagcaggccg cccgtactac ggcgaagccg
     1621 gagctcctga atccagggac cacacagaga ttacagattg tgcagctttc atctccgttt
     1681 agtccgagta gtctgtggtt taccatcgtc taaattgacc ggtgccggat aaccttttcc
     1741 cgcaagagta ctcacgtcga tcacgacttc agtcttgact ggtttctgca aggcctcgag
     1801 cgcttgcctc cgctgatctt cggaaagatc ctcgaggcgt gcgccgtgca tttccagcac
     1861 gcgacgcagg ttctcttcag tattcgtgtc ctgtgcatag acgtctttgt agatgtaaag
     1921 cttgccatcc tccagcacga tcgtctcgta acgaagttca accggaatca cctggtccag
     1981 tttcaatgtc ttcgtttgag tcttgtccgc gagatactgc gtgatctcct tgtcggagac
     2041 ctcgttgttt gcggcgcgga tcaactgcgt ggcgaagtct ttcacctgcg cattcgtcat
     2101 accaacacaa ccatgcgacg caaactttcc tatcttcgcg ggcgccttgc ctccatgaat
     2161 caacgagggc atgccgatcg gtatcttgat gatgccgagt ggattgtctt tgctgccggg
     2221 ttgcactgtc tcgcccgggg ttacgttttt catcttcgcc acccacggtg aatgaggcgg
     2281 tgtccacggc ggattaaaaa tcaccgtctg cgccttgcgc agtccaagcg gcagtggaaa
     2341 ctccggatat ccgatgccga tcttgtacga cttcacgagc gagccattct gaaagaggtc
     2401 catgcgaaac gccgggatgt tgacgacgac gcgtgtgtct gccggcacgg cgtttggtcc
     2461 tttcaacatc tcgaatgcgg cgccattcgc accctcagcc tcgggatcgc ctttggccca
     2521 aaactcgttt gccagcgaag cgacttgtgt cgctttctcg tcgccgagga tggagcgtag
     2581 ttgttcggcc gcgcgcgtgg gagcgtcggt gccgttgccg tttatctctt ccgcgtcttc
     2641 cgcgttagcc tcgcgcagtc gatcgacctc caattgcgac gcgcgtttga gtgaagcaat
     2701 ccgatcgtct gaaagatttg ccttgttctt tagctcgctc acaaatgcat cgtccgccag
     2761 tagtgcgtcg agcaacggca gcgtcacctg gaccgatgcc tggtccgtcg acgccggtga
     2821 agcagccggc gatggagcgg gtgtcgcttg agttactggt gcgtttgaat tgttcgtcgg
     2881 tgccgtgcag gcgcttccga ctgctaatgc tgttgcgatg agactgaaga gtaaaagacg
     2941 cggcatcatg gtcataaaca ggttctctct ttcggtaggc cagactttta gtctgtccat
     3001 gacaaggcaa gaatgcctgt cctacgggga gctacggatg tgaggcgagc aatgagcgtt
     3061 cccacacaga gatgcgtctt ttcccacggg tgaatcgatg agcagccact tttatgaacg
     3121 attaacggac agggtgaaca aagatcgtgc gggagatggg acgtggggct aatggcgcag
     3181 cagcaagtaa ggattgatgt tcgttccttc ccaataacgt ttgggatctg aaacgatcgc
     3241 gatggaaaag tggaggtggt agttgccaat tccggcgttg ccggagtcgc ccacatacgc
     3301 gattgtttcg ccctggcgca cctgctttcc ttcagttaga ccatcggcgt aacgcgcgag
     3361 gtgcgcgtaa tagaagatta atttctggtc agcgctgagt tgataaatgg tggtgccgcc
     3421 gcgttcgctt tgaaagagtc tctgaatctg gccgtcagcc gcagccaata cgggagtttc
     3481 ggctggggcc atgatgtcga ttgcatcgtg agtgcggcct tcggatcggc tgtcagagaa
     3541 tgtgtccgtc aactgatccg gcctgacacc ggcgaccgga ataataagtg gttctatccg
     3601 tgctatctgt gaaaccggtt gggccacggt ttgaaccggc tgaaccacag acggcacgga
     3661 ttcgaccagg gaaggcggtg gaacacgtcc tgttcccagc cacaccaggg caataaacgc
     3721 tagtaaaaac accaaaaata gtacttgcaa ccggttcatc accggtttta gtaacaacta
     3781 acgttccaag ccgagcggta aaagtggccg tggcgcggtt ccttcaaaga accagctcgc
     3841 gccgacgcga agccggtgat gccacgtagg cagacgagag gtgtaaagcg cgaagcgcgc
     3901 ttgtcgcgcg agtgcaccgc cgaacgcttt tccggccgtc agcaccgccg cgcgttcagt
     3961 accaaggctg atcgcttcgc cgagctcttc gaagtgtttg gttcgtagtt ccttgccttc
     4021 gagaaacgcc ttgacgttat gaccggccag gccggcttcc tgaaaggcca gttgcgcagt
     4081 gccgtggagc gtaggctccg catccgggta gaaagcgatg tcgccaagcg cgaacacgtt
     4141 atcgtgttta gagagttgca gggttggtct gatgagtagc aaaccgcggt tgttcttttc
     4201 gacgtccaac cgttctacga gcggattcat acgcacgcca ccgagccaga ctaccgcggc
     4261 tgtctttacc tgctcctgtt ttccctcgtg ttcgaacgtc acgccttcgc gtgtgacgtt
     4321 gatcacgcgc gttaacgtgt gcacttccac gtgcgactca cgcaaagctt cttcgaccag
     4381 ttcgcgaatc tgttggccca ttcccggaac cacgcggtca cccatttcga gcagcatcac
     4441 tcgtggctca ccgtgaagcg cgcgtcgttt gaaagcgtca atgaggaggt cggccatttt
     4501 ggtcgagagt tcgcagccac tggcgcccgc gccaacaagc gcaaacgtta gctcgcgtcg
     4561 cacatcctgc ggcggcattt caggtggaat gcgatcgagt gcatcgacca tcatccggcg
     4621 cagcgtgtcg gcatgttcga tcttgcgaaa gggcagagaa aactcttcgg cgccgggtac
     4681 gcctgcgtag ttcgtcactc cgccgacagc gaggaccagg acatcatatt tcagcgcttc
     4741 ggcctgctcc ggagtgacag tctgcgcgtc gaagttgacg gttgcaactg ggctcttgac
     4801 gagttttacg tctttgtgga gcagttcgtc atatcgcgga ccgatatgcc aggcctcgac
     4861 ttcgccgctg aaatactcgt aaagcatggg tgtgaaaacg aagtgatccg agtcggtgac
     4921 gagggtgacg tcatgagagc cggaaagttc gagggcggtg aagagtcccg caaagccgcc
     4981 accgacgacc acgattctgg gctgtgccat gcgccgcata gtaaacccgc gccctgaaag
     5041 gcacaagcga cacaaatttt gtccccggga gaacggcagc cgccgtggtg tcctaatctg
     5101 aacgccgaag gcgttatcga attcgagccc agaggttgga gcttgcgaca accctgggga
     5161 ctgattggat gtttttacct ttacgctgaa agcgttggct aaccgcgaat ttgccaacgc
     5221 cttcagcgtt aggacgggac gctaacgttc gacccagggt tgaagcaacc ctgggctgga
     5281 attagcgaac gcctggcgtt cagattagga ctactctctg ggcgctgccg ttggttttgc
     5341 aggggaaacg cctgcggtcc aggttaaagc gggtttgcca cgaaaagtga t
//
LOCUS       DASTEY010000542         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_490115, whole genome shotgun sequence.
ACCESSION   DASTEY010000542 DASTEY010000000
VERSION     DASTEY010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490115"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFO72_09495"
     CDS             complement(<1..184)
                     /locus_tag="VFO72_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479568.1"
                     /translation="MTEALNEGREMIKGLDLLFLEVNSLEESLAFYQNVLAFDVESIS
                     LNSEPPMARLRGGQLKI"
     gene            complement(181..735)
                     /locus_tag="VFO72_09500"
     CDS             complement(181..735)
                     /locus_tag="VFO72_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9479569.1"
                     /translation="MSQSDSDKDYYGVLGVDKHASQREIDRQYKREASRHHPDRGGSE
                     ERMKSLNEAYGVLKDSNSRRSYDESRKTTAKVGDFVPVSTPTARDVGVLGQCLSALLC
                     LVAGLFLLLLVRIQWIWFLWPLAILAVAVVGFGVLLARSAMVAVNDSLPVTNRLKRHT
                     KLQEVAFWTVIVTTGYGLYLLLSY"
     gene            801..1154
                     /locus_tag="VFO72_09505"
     CDS             801..1154
                     /locus_tag="VFO72_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479570.1"
                     /translation="MPEFAPGDDLIFQLESGFGLLRVLAVEGEGVETVWHLLAYDDFF
                     PDVESAETALGGSQPLPVRRAHMALTNRAFERTPAARLGNRAVSEDELVEYRRWLDSD
                     RVVSDRSALLMLGIR"
     gene            1169..>2067
                     /locus_tag="VFO72_09510"
     CDS             1169..>2067
                     /locus_tag="VFO72_09510"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479571.1"
                     /translation="MKKPLLSKPAQLSLLLASFLIATSLAYGARVERLIDSWQPKHYL
                     LNITLDDQLSAIVSATARIDVLILKPTTLVDLDFGELTVDAVTVDSKPASFTHRDAKL
                     KINLTAPASPGTRLQISINYHGTPKDGLILTKDKDGRPSAVGDNWPNRVHHWIPTLDH
                     PSAKATITFNITAPADNEVVANGRLDQVQTTSNGNRTWTYSGGVPIPPYCMIIGVGQF
                     KRLEPAERSLTPLSYYVPHSDAPFALKGFAPSIPSLAYFSETIAPYPYEKLAMIVGAT
                     RFGGMENSSAIVFTSTLFNPKPTA"
BASE COUNT          474 a          606 c          518 g          469 t
ORIGIN      
        1 tgattttcag ctggccgcca cgaagtcgcg ccatcggcgg ctcggaatta agactaatcg
       61 attcgacatc aaacgccagc acgttttgat agaaagcgag tgattcttcg agactgttga
      121 cctcgagaaa cagcagatcc aggcctttga tcatctcgcg cccttcattc agtgcttctg
      181 tcaataagag agcaacaggt aaagtccgta gccggtggtg acgatgacgg tccagaacgc
      241 gacctcctga agcttggtgt gtcgtttcaa acgattcgtc accggcagcg aatcattcac
      301 tgccaccatc gcgctgcgcg ccagcaacac gccgaaacca accacggcca ctgctagaat
      361 cgcgagtggc cacaaaaacc agatccactg aatgcgaacc agcaacagca gaaacaagcc
      421 cgcgactaaa cacagcaacg cgctgagaca ttgacccagc acgccgacgt cgcgtgctgt
      481 aggcgtcgaa acaggaacaa agtcaccgac ctttgcggtg gtctttctcg actcgtcgta
      541 tgaacggcgc gagtttgaat ctttcaaaac accgtacgcc tcattaagcg acttcatccg
      601 ttcttcactg ccaccgcgat ccggatgatg tctcgatgct tcgcgcttgt actgccgatc
      661 gatctcgcgt tgcgaggcgt gcttgtcgac gcccaaaacg ccatagtagt ctttgtctga
      721 atcggactgg ctcatcgcga attgcctgat tctagaggcg ccattttcgt ttggcaatgt
      781 gtgttatgcg tgtttaatgt atgcctgaat ttgcacccgg tgacgatctg atcttccaac
      841 tcgaaagcgg cttcgggttg ttgcgcgtgt tggctgtcga aggagaaggc gtcgaaactg
      901 tttggcactt gctcgcctat gacgacttct ttcccgacgt ggaatcagcc gaaaccgcgc
      961 ttggtggttc gcagcccttg cctgtgcgcc gagcgcacat ggcgctgact aaccgtgcat
     1021 tcgaacgcac gcccgcggca cgtctcggta atcgtgctgt gagtgaagat gagttggttg
     1081 aatatcgccg atggctggac agtgacagag ttgtttctga ccgttcagca ctactgatgt
     1141 tgggaatccg ctaaatttag ttttctaaat gaaaaaacct ctactctcta aacctgcgca
     1201 actctcgctc ctattagcct ccttcctaat tgcaacttcg ctcgcgtacg gcgcgcgcgt
     1261 cgagcgactc atcgattcgt ggcagccaaa acattacctg ctcaacatca cgctcgacga
     1321 tcaactctct gcgatcgtct ccgccaccgc gcggatcgac gttttgatcc taaaaccgac
     1381 gacgctcgtc gatctcgatt tcggcgagct gaccgtcgac gcagtcactg ttgactccaa
     1441 accggcttca ttcactcacc gagatgcgaa acttaagatc aatctcactg cacccgcctc
     1501 gcccggcacg cgattacaaa tctccatcaa ttatcacggc acgcctaaag acggattgat
     1561 cctcaccaaa gacaaagacg gaagaccatc cgctgtcggc gacaattggc ccaaccgcgt
     1621 ccatcactgg attcccacac tcgatcatcc gtccgcaaaa gcgacgatca ctttcaacat
     1681 cacggcgccg gctgataatg aagtcgttgc gaacggacgc ctggaccagg tgcaaacaac
     1741 gtcgaacggc aatcggacct ggacttactc ggggggagtg ccgattccgc cgtactgcat
     1801 gatcatcggt gtgggccagt tcaagcgttt ggaaccggct gagcgttcgc tgacgccgct
     1861 ttcgtactac gttccgcaca gcgacgcgcc atttgccctc aaaggttttg cgccttcgat
     1921 tccctcgctg gcgtatttca gcgagacgat cgcgccgtat ccgtatgaga agctggcaat
     1981 gattgtcggc gcaacgcgtt ttggcgggat ggaaaactcg agcgcgatcg tgtttacaag
     2041 cacattgttt aatcccaaac cgacagc
//
LOCUS       DASTEY010000543         4853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_836463, whole genome shotgun sequence.
ACCESSION   DASTEY010000543 DASTEY010000000
VERSION     DASTEY010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836463"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /gene="dxs"
                     /locus_tag="VFO72_09515"
     CDS             complement(<1..1906)
                     /gene="dxs"
                     /locus_tag="VFO72_09515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET9479572.1"
                     /translation="MSILETINSPADLRLLSLDQLRTVSDEIRNYILETMSRVGGHTG
                     ASLGAVELAVALHYAFQTPKDRLVWDVGHQAYAHKILTGRRELLPTIKQYGGISGFLR
                     RDESEYDTFGAGHASTSLSAALGMAIARDRKGDDYHVVALIGDASLAGGMAMEAINQA
                     GHLKSRLIVVLNDNEMSIAPAVGALTGYLNRIRGAHGYHRFKDEVEAKLLAIPSVGER
                     LHHAAKTMKDFVAAAVLPGALVSELGFKYIGYVDGHNPRALVAAFNEAKHIKDGPVIV
                     HALTTKGKGFPQLEPDYYRWHATGPFDLKTGEAIKSSSKAPTYTSVFGDVLCELMEKD
                     PQIVALTAAMPDGTGVCKALERFPERSFDVGIAEQHCVTFAAGMSCEGLKPVCAIYST
                     FLQRGFDQLIHDVCIQDLNVKFCLDRGGIAGGDGQTHHGLLDIAYLRAIPNIIVMAPK
                     DEGEMRDMLYTMLEHTGPAAMRYPRGNGVGADIEREPQLLEIGKGEILRDGGEIAIVA
                     YGAMVHPSLEAAEQLATKDRIEATVVNARFVKPLDAPLLLALARTKRLIVTVEEAYLA
                     GGLGSAVLELLEENGLQDKVRVVRMGIPDRLITHGDAKLLLAKYGLDVDGIYNRVKES
                     LEVLDEWRAKP"
     gene            complement(2195..3043)
                     /locus_tag="VFO72_09520"
     CDS             complement(2195..3043)
                     /locus_tag="VFO72_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9479573.1"
                     /translation="MPELSPTRRQFLRGLVAAPLIAVAGTSAYASLVEPYHYWVSETD
                     VFIRNLPERFEDFRITQLTDIHHSRILGIDQVRRVVELAQQTSPDMFVLTGDYTTTYR
                     RFIEPCAEALAGLKAPYGVWAVLGNHDHYTDPELTTRALRRQRIVVLDNVYATLERGP
                     DSLQLVGIDDWTWNAADWTRAFTGLRPDRPTILLSHQPSVLDFEQTRNVALILSGHTH
                     GGQLRLPVVGAPARFATDALKYDRGLFRRGETQLYVSSGTGVIGLPLRLGVRPEIAVL
                     RLKRKY"
     gene            3113..3203
                     /locus_tag="VFO72_09525"
     tRNA            3113..3203
                     /locus_tag="VFO72_09525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3147..3149,aa:Ser,seq:gct)
     gene            3256..4752
                     /gene="gltX"
                     /locus_tag="VFO72_09530"
     CDS             3256..4752
                     /gene="gltX"
                     /locus_tag="VFO72_09530"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET9479574.1"
                     /translation="MTVRVRFAPSPTGYLHIGAARSALFNWLYARKMHGKFLLRIEDT
                     DLQRSTEESTRSIIEGLQWLGLDYDEDLVFQSANAPRHRAAAERLVQEGKAYRDFTLK
                     EQRDDKSVKEDVAERARAQTAEGVDPRSNPFRDLPKEESDRRAAAGESFAIRLKVQQT
                     GQTRFNDIVYGPQERNHSEIEDLVLLRSDGHPLYNLSVVIDDIKMGITHVIRGQDHLT
                     NTHKQILLYEALDAKIPQFAHLPLILAPNKGKLSKRKHGEVVSLTTYRDRGFVPAAFR
                     NFLALLGWSPPEGKEILSKEELVEYFSLEGIGRANAIFNFHENDPRRWTDDKALWMNA
                     EYIRTMPIEELLPMVKGELRANKLWREEYDDDERDWLIKTVDLIRERYFTLKDFSSQG
                     RAYFSGDFDFEEAAVNKNLRKEPRLRELLHGLADKLETVEPFNAETAEAALRAYADEA
                     GVKAGLLINGSRTMLTGQAVGPSMFEVFDVLGKEKSIERLRSGIPWSE"
BASE COUNT         1049 a         1379 c         1383 g         1042 t
ORIGIN      
        1 cgggtttcgc gcgccattcg tcaagaactt ctagactctc tttcacacga ttgtaaatgc
       61 cgtcgacgtc gagcccgtat ttcgccagca acagcttcgc atcgccgtgc gtaattagcc
      121 gatccgggat tcccatacgg actacgcgca ctttgtcctg caacccgttc tcttcgagca
      181 actcgagcac tgccgacccg agcccgccgg cgaggtaagc ctcttcgacg gtaacgatca
      241 gacgcttcgt gcgtgctaac gctaacagca gcggcgcgtc gagcggttta acgaatcgcg
      301 cgttcacgac cgtggcctca attcgatctt tggtcgcgag ttgttctgct gcttcgagtg
      361 acggatggac catcgcgccg taggcgacaa tcgcaatctc accgccatcg cggagtatct
      421 cgcctttgcc gatttcgagc agttgtggct cgcgttcgat atcggcccca acgccattgc
      481 cacgcggata gcgcatcgcc gccggcccgg tgtgttccag catggtgtag agcatgtcgc
      541 gcatttcgcc ttcgtctttg ggcgccatca cgataatgtt gggaatcgcg cgcagatacg
      601 cgatgtcgag cagtccgtgg tgagtttgtc cgtcgccacc ggcaatgccg ccgcgatcaa
      661 gacaaaactt cacgttcaga tcctgaatgc agacgtcgtg gatcaactga tcgaaaccgc
      721 gttgcaggaa cgttgaatag atggcgcaga ccggtttgag tccttcacac gacattccgg
      781 ccgcgaacgt gacgcagtgt tgctcggcga tgccgacgtc gaaagagcgc tcgggaaacc
      841 tctcgagcgc cttgcaaacg cccgtgccgt cgggcatcgc cgcggtcaac gccacaattt
      901 gcggatcttt ctccatcaac tcgcaaagca cgtcgccaaa aacagacgtg taggtgggcg
      961 ctttagacga gcttttaatc gcctcgcccg tcttcaaatc aaatggcccg gtcgcgtgcc
     1021 agcgatagta gtcgggttca agttgcggaa agccctttcc cttggtcgtg agagcatgaa
     1081 cgatgaccgg cccatcctta atgtgcttgg cttcgttgaa agctgccacc agcgcgcgcg
     1141 gattatgtcc atcgacgtag ccgatgtatt tgaatcccaa ctcgctcact aacgctccgg
     1201 gcagcacagc ggcggcgacg aagtctttca tcgtcttggc tgcatgatgc aggcgctcgc
     1261 ccactgacgg aatcgccaag agcttcgctt ccacttcgtc tttgaatcga tggtagccgt
     1321 gcgcaccgcg aatgcgattg aggtatccgg tcaacgcgcc tacagcaggc gcgatagaca
     1381 tctcgttgtc gttgagcacc acaatgagcc tgctcttcaa gtgaccggcc tgattgatcg
     1441 cctccatagc catgccgccg gcgagtgaag cgtccccgat caaagccacg acgtgataat
     1501 cgtcgccttt acgatcgcgt gcgatcgcca tgcctagcgc tgcggaaaga gacgtcgaag
     1561 catgaccggc gccaaacgtg tcgtactccg attcgtctcg gcggagaaac ccgctgatac
     1621 cgccgtactg tttgatcgtc ggcaaaagtt cgcgacggcc ggtcaggatc ttatgcgcat
     1681 aagcctggtg accgacgtcc caaacgagcc gatccttcgg cgtctggaag gcgtagtgca
     1741 acgccactgc caactcgaca gcgccgagcg aagcgccggt gtggccgccc acgcgagaca
     1801 ttgtctcgag aatataattg cggatttcgt cggaaacggt tcgcagttga tccagggata
     1861 aaagacgcag atcggcaggc gagttaatag tctcaagaat gctcattgtg gccgcattgt
     1921 actccaacca tcggttttgt ccgttcgtca acgtacttgg cgatataaag tactctgtta
     1981 aagtggacga cgtcaactga attttcgttt ccgtcgggtg tgctgtttga acggggagcg
     2041 tcgatttctg tgacgcgctc ttccccgagg accgcaggcg tctcgcctgc aggtcacgaa
     2101 tcgggcgctc acgtgtcatg caggcgagac gcctgcggtc ccagggaaga gcacgcttaa
     2161 gtaactgtcc actcccaagt gtgggggaac tgcatcaata ctttcgtttt aggcgcaaga
     2221 cggcaatctc cggccgcacg ccaagccgca gcggcaggcc gattacaccc gttccactgg
     2281 aaacgtacaa ctgcgtctcg cccctgcgga aaagcccgcg atcgtatttc agcgcgtctg
     2341 tcgcgaagcg tgccggcgcg ccaactaccg gcaagcgcag ttggccgccg tgggtgtggc
     2401 cgctcagtat caacgccacg tttcgcgtct gttcaaagtc gagcacgctc ggctggtgcg
     2461 aaagcagaat ggttggtctg tctggtctca agccggtgaa ggcgcgtgtc cagtcggctg
     2521 cgttccacgt ccagtcgtca ataccgacga gctgaagcga atctggtccg cgttcaagag
     2581 tcgcgtagac gttatccagc accacgatgc gctgacgtcg caaagctcgt gtagtcagtt
     2641 ccggatcggt gtagtgatcg tgattaccga gaactgccca cacgccgtaa ggcgccttca
     2701 agccagcgag tgcttcggcg cagggctcga tgaaacgccg gtaagtggtt gtgtaatcac
     2761 cggtgagcac aaacatgtcc gggctggtct gttgggccag ttcgacgacg cggcggactt
     2821 gatcgatgcc gagaatacgg ctgtgatgaa tatcggtcag ttgcgtgatg cgaaagtctt
     2881 cgaatcgttc gggaagattt cgaatgaaga cgtcggtttc cgacacccag tagtgataag
     2941 gctcgaccag gctggcgtac gctgaggttc cagcaactgc tatgagcggc gccgcaacta
     3001 aaccacgaag gaactggcgg cgagtcggcg ataattcagg cacgcgtcga ttctacctca
     3061 ccttgacaat ccgccttccc cgactagaat tggacttcgc ctgatccggc acggagatgt
     3121 ggccgagtgg ctgaaggcgg cggtttgcta aaccgttaag ggtcaaaagc ccttcacagg
     3181 ttcgaatcct gtcatctccg ccagacaatt tcagtagtca gttgtccaag cacggatggc
     3241 tgactacttt ttattatgac tgttcgcgta cgcttcgctc cgtccccgac cggctaccta
     3301 cacattggtg ccgcccgatc agctctcttc aactggctct acgcgcgcaa gatgcatggg
     3361 aagtttctct tgcgcatcga ggacaccgat ctccaacgtt caactgaaga atccactcgc
     3421 tccatcatcg agggattgca gtggcttggt cttgattacg acgaagacct cgtcttccag
     3481 tcggcaaatg cgccacgaca tcgcgccgcc gccgagcgtc tggtccaaga gggtaaagcc
     3541 tacagagatt tcacgctgaa agagcagcgc gacgataaga gcgtaaagga agatgtcgcc
     3601 gaacgcgcgc gggcgcagac ggccgaaggc gtggatccgc gcagcaatcc gtttcgcgat
     3661 ctgccaaagg aggaatccga ccggcgtgca gccgcaggcg agtcgtttgc gatccgtctg
     3721 aaagtgcagc agaccggcca aacccgattt aatgacatcg tctacggtcc acaagagcgc
     3781 aatcattccg aaatcgaaga cctcgtcctg ttacgctccg atggtcatcc gctttacaac
     3841 ctctcggtcg tgatcgacga catcaagatg ggcatcacgc acgtgattcg cggccaggac
     3901 catctcacaa atacgcacaa gcagatcctg ctttacgagg cgttggacgc aaaaatcccg
     3961 cagtttgcac atctgccgct gatcctcgcg ccaaacaaag ggaagctctc gaaacgaaag
     4021 cacggcgagg tggtttcgct gactacgtat cgcgatcgtg gttttgtgcc ggccgcattt
     4081 cgcaattttc tcgcgctgct cggatggtcg ccgcccgaag gcaaagagat actcagcaag
     4141 gaggagttgg tcgaatattt ctcgcttgaa ggcatcggcc gggccaacgc gatcttcaac
     4201 tttcacgaga acgatccgcg gcgctggacc gacgacaaag cactgtggat gaacgccgaa
     4261 tacattcgca cgatgcccat cgaggagttg ttgccgatgg tcaaggggga gttgcgggca
     4321 aacaagttgt ggcgcgagga gtacgacgac gatgagcgcg actggttgat caagactgtc
     4381 gatctgattc gagagcgcta cttcacgttg aaggattttt catcacaggg gcgggcgtat
     4441 ttctccggcg acttcgattt tgaagaagcc gccgtgaaca agaacctgcg caaggagccg
     4501 cgtttgcgcg aactgttgca tggtctggct gacaaactcg aaaccgtgga acctttcaac
     4561 gccgaaactg cggaagcggc gttgcgcgca tatgcggatg aggccggcgt gaaagctgga
     4621 ctattgatca acggttcgcg cactatgctg actggtcagg cggttggccc atcgatgttt
     4681 gaggtgtttg acgtccttgg aaaagaaaaa tcaatagagc gattacgcag cggaatcccg
     4741 tggtcggaat aaggagttgt ttatgaaaac acgaattttt ctaatactcg tttttatgtc
     4801 cgtgacatcc gtggtcgctt ttgctcaggc tcctactggc tccgaagccg tgc
//
LOCUS       DASTEY010000544         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_737071, whole genome shotgun sequence.
ACCESSION   DASTEY010000544 DASTEY010000000
VERSION     DASTEY010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737071"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFO72_09535"
     CDS             <1..1039
                     /locus_tag="VFO72_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9479575.1"
                     /translation="NIPNAATRFQHFAAGQMISSPTAEEAVVTERFIRDYGFEKAADA
                     VGKTIELLAPASEKSDNQEQTEEEESPNFFGIPLDDPGLDESAPGLETRTFRIAGVLS
                     TEIREGAGQGGMRGMMPSAGIYIPLSIAHDWTTKHRTPMSQVALALARRGGNLGEGQT
                     EGYDSAVVRVTDPVKLQEVRQKISDLGYGSFSIVDELDQIRTVFLIIDSVLGLLGGIS
                     LLVASFGIANTMIMSIFERTREIGIMKAIGAEDREIKLIFFFEAAVIGVTGGVIGVLL
                     AWGIDGLANRLAYRFVLKPQGASFIDFFDLPWYLTVGAILFALMVSIVAALYPAARAA
                     RIDPVRALRHD"
     gene            complement(1125..1691)
                     /locus_tag="VFO72_09540"
     CDS             complement(1125..1691)
                     /locus_tag="VFO72_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9479576.1"
                     /translation="MDSRYYPNVEIDVAQLTTELRSLFDSEFEVQTFQVSTTSIVQAR
                     KSNTLRDLTGLSSALTIKITPEPGGTRVEIGMQKWFDKAAVAAVGAIVFAPLLALAAL
                     GAYWQYKLTEDAWKIIEDHIARKAGGYVPPLPGRCGTCGAANVAGSDFCSSCGANLRV
                     GRNCPSCGVAQKDISARFCNRCGTALAG"
     gene            1733..>3344
                     /locus_tag="VFO72_09545"
     CDS             1733..>3344
                     /locus_tag="VFO72_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET9479577.1"
                     /translation="MRTFLILLLQILLLAPPAFSQTAAIDGFSAERAMAHRRWEEQFR
                     AVPAPKSAREHLRLLTLEPHMAGTKEDYDTAIYVRDQLRSYGIAAELKEYDVWLNYPN
                     SPTVLELISNKRRQKLKTHEDVVSGDPTSSHPKITPLFNGYSASGDVTAPVVYANYGL
                     PHDYEDLKKAGVDVKGKIVIVRYGNSFRGVKAKVAQDHGAIGCIIYSDPADDGYMQGD
                     VYPKGPWRPGASGQRGSVQYLFDYPGDPLTPGRPAIDGVPRLKPEEATDLTRIPVQPI
                     AYDVAKQIIAPLKGPVRPRGFQGGLPFAYHVGGTDDVKVRLRTDMDYKLRKIWNVIAR
                     IDGTDQSDRWVILGNHRDAWVFGAVDPNSGTSAMLEVGRGLGELLKRGWKPRRTIVLC
                     SWDAEEYGLIGSVEWVEELAAELKEKAVAYLNLDAAVSGVNFGASTVPSLWKLMRAVT
                     REVKDPKTGKTVYQQWQDRAREARPEDERATAGEARIGPLGSGSDYTPFLQHLGIAST
                     DMGFGGDYGVYHSAYDSFYWMDRFGDPGF"
BASE COUNT          700 a          886 c         1019 g          739 t
ORIGIN      
        1 aaatattccc aacgccgcaa ctcgcttcca gcacttcgcc gcgggacaga tgatttcgtc
       61 gcccactgct gaagaagcgg tggtgactga acgctttatt cgtgactacg gttttgagaa
      121 ggcggctgat gccgttggga agacgattga gttgctggcg ccagcgtcgg agaagagcga
      181 taaccaggag cagacggaag aggaggagag tccgaacttc tttggtattc cgttggacga
      241 tcccggactc gatgaatcgg cgcctggatt ggagacgcga acgtttcgta ttgccggagt
      301 tttgagtacg gagatcaggg aaggcgcggg tcagggcggc atgcgtggca tgatgccgag
      361 cgccggaatt tatatcccgt tatcgatcgc ccacgattgg accacgaagc atcgtacacc
      421 gatgagccag gtggcgctcg ccctggcgcg tcgcggaggg aacctgggag aaggtcaaac
      481 tgaaggctac gactccgcgg tagtgcgcgt gacggatccg gtgaaactcc aggaagtgcg
      541 tcagaagatc agcgaccttg gctatggcag tttttccatc gtcgatgaac tggaccagat
      601 tcgcaccgtg tttctgatca tcgactcggt cttgggacta ctgggcggca tatcgctgct
      661 ggtagcgtcg tttggaatcg caaacacgat gatcatgtcg attttcgaac gcactcgcga
      721 gatcgggatt atgaaagcga tcggcgccga agatcgtgag atcaagttga tctttttctt
      781 cgaagcagct gtgattggtg tcactggcgg agtgattggc gtgttgctag cgtggggaat
      841 tgacggcctg gcaaaccgtt tggcgtacag atttgttctc aaaccgcagg gcgcatcgtt
      901 tatcgacttc ttcgatctcc cgtggtatct gaccgtcggc gctattctct tcgcgctaat
      961 ggtctcgatc gtcgcggccc tctatcccgc cgcccgcgcc gcccgcatcg atcccgtccg
     1021 cgcgctgcgg cacgactgaa ggaaccccgc aaaggctcaa aagagggcaa gaagacatcc
     1081 gtttcgccgt cttcttttcc tttgcgcctt tgcgtgaatc tgtcttatcc cgccaacgca
     1141 gttccgcagc gattacagaa acgcgccgag atgtctttct gcgcaacgcc gcaggaggga
     1201 caattgcgcc caacgcgcag gttcgcgccg catgaggaac agaaatctga gcccgcaacg
     1261 tttgctgcgc cgcacgtgcc gcaacggccg ggcagcggcg gcacgtagcc gcccgctttg
     1321 cgcgcgatgt gatcttcgat tatcttccac gcgtcttctg taagtttgta ttgccagtat
     1381 gcgccgagcg cagccagcgc caacaggggg gcgaagacga tcgcgccaac ggcagcgact
     1441 gcggctttgt cgaaccactt ctgcatgcca atctcaacgc gtgtcccgcc gggctcgggc
     1501 gtgatcttga tcgttaacgc cgaagagagt ccggtcaggt cgcgcaaagt gttcgacttg
     1561 cgcgcctgga caatgctggt ggtcgacacc tggaaagtct gcacttcaaa ttcgctgtca
     1621 aagagacttc gcagttcggt tgtcagttgc gcaacatcta tttctacgtt tgggtaatat
     1681 cggctgtcca tcaatcagcg tcctttcgat gcagactcga gtgcattgta ctatgagaac
     1741 ctttcttatt cttctactcc agatactcct gctcgcccct ccggcttttt cacagaccgc
     1801 ggcgatcgac ggcttcagtg ctgaacgcgc aatggcccac cgccggtggg aagaacaatt
     1861 tcgcgcggtg ccggcgccga agtctgcgcg tgaacatttg cgtctgttga cgctcgagcc
     1921 gcacatggcc ggcactaaag aagattacga caccgcgatt tacgtgcgcg atcagttgcg
     1981 tagctatgga attgcggcgg aactgaagga gtacgacgtt tggctcaact atcccaactc
     2041 gcctaccgtg ctcgagttga taagtaacaa gcgtcgccaa aaactgaaaa cacacgaaga
     2101 cgtggtatcg ggcgatccaa catcgtcgca tccaaaaatt acgccgctgt tcaatggtta
     2161 cagcgcgagt ggtgacgtaa ctgctccggt tgtttacgcg aattatggtc tgccacacga
     2221 ttacgaagat ctgaagaaag ccggcgtcga cgttaaaggc aagatcgtga tcgtccgtta
     2281 tggcaattcc ttccgcggcg tcaaagcaaa ggtcgcacag gaccacggcg cgattggctg
     2341 catcatctat tccgatcccg cagacgacgg ttacatgcag ggcgacgttt atccgaaagg
     2401 accctggcgt ccaggtgcgt ccggtcaacg tggctcagtg cagtacctgt ttgattatcc
     2461 gggcgatcca ttgacgccgg gtcgaccggc aattgatggc gtgcctcgat tgaaaccgga
     2521 agaggcgact gacctcacgc gtataccggt gcagccgatc gcgtacgacg ttgccaaaca
     2581 aatcatcgcg ccgctgaaag gacctgtgcg gccgcgcggt tttcagggcg ggttgccgtt
     2641 cgcgtatcac gtcggcggca ctgacgatgt gaaagtccgt ctcaggacgg acatggatta
     2701 caagctgcgc aagatctgga acgtaatcgc gcgaatagat ggcacggatc agagtgaccg
     2761 ctgggtgatt ctcggtaatc accgcgacgc ctgggtgttt ggagccgtag atccaaacag
     2821 cggcacgtcg gcgatgctgg aagtgggtcg cggtttgggt gagttgctca agcgtggttg
     2881 gaagccgcgt cgcacgatcg ttctgtgcag ttgggatgcg gaggagtatg gcctgatcgg
     2941 ttcggttgag tgggtggaag agttagccgc cgaactgaaa gagaaagcgg ttgcgtattt
     3001 gaatctcgat gcggcagttt ccggcgtgaa ctttggcgca tcgacggtgc cgtcgttgtg
     3061 gaaactgatg cgcgcggtga cgcgtgaggt caaagatccg aagaccggca agactgtcta
     3121 ccagcagtgg caggatcgtg cgcgcgaagc gcgtccggaa gatgagcgag caactgccgg
     3181 ggaagcccga attggaccac tcggttcggg ttcagattac acgccgtttc tgcaacatct
     3241 aggaattgca tcgacggata tgggcttcgg tggtgattac ggcgtgtacc actcggcgta
     3301 cgattcgttt tattggatgg atcgctttgg cgatccgggg ttct
//
LOCUS       DASTEY010000545         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_951039, whole genome shotgun sequence.
ACCESSION   DASTEY010000545 DASTEY010000000
VERSION     DASTEY010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951039"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFO72_09550"
     CDS             complement(<1..485)
                     /locus_tag="VFO72_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479578.1"
                     /translation="MLIIAVSTFICIAFGVMAIYWLMFRPVSATAARLKELGDAQGSV
                     AASIEPNSVASLAERLAEPINRLVPPSAANAKKLQKDLMQAGFRSPHAPSVYRASQFL
                     SMLVCPLLVLLVYTFLVWPLNGALMPGLFAFGAGYILPRLILNRLIAARKLRITWGLA
                     DA"
     gene            complement(519..1487)
                     /locus_tag="VFO72_09555"
     CDS             complement(519..1487)
                     /locus_tag="VFO72_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9479579.1"
                     /translation="MLLIPFFVFIACLFLKYALYLITSRSSDKKRALLNERLAEAIRS
                     SVNSADMDVQLAREELLSEIPWLNRSLLKLAIPGRIKRTIDQADLNITVVRLVLFSLT
                     AGVLAFLAVSMVSSSYLLMTLCGVIAAILPYSHVLAKRKKRLNKFLQLLPDALDLMSR
                     GLTAGHAFTESLHMVSTEMPEPIATEFRKTYEEQNLGLSLKLALENMVQRMPLLDLRM
                     CVTAILIQRETGGNLSELLEKVAYTIRERFRILEDLKTLTLSSRWSAWLLCALPILLA
                     IYISVMNPGYMDVLWRDPRGQKLIAVAVTMQILGMLMVQRIMKIKI"
     gene            complement(1487..>2327)
                     /locus_tag="VFO72_09560"
     CDS             complement(1487..>2327)
                     /locus_tag="VFO72_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HET9479580.1"
                     /translation="GSRVNAIIPPLSLDGPVLSIRRFGAEPLRMNRLLENQALTPDMA
                     SMFEMCVTARLNILISGGTGAGKTTLLNAMSAYIPSDERIVTIEDSAELQMQQPHVVR
                     LETRPPNIEGKGEVSQRDLVRNALRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTT
                     IHANTSRDALARLETMIQMTGMRLSDRAMRQQIASAINMVIQVARLSDGTRRIISISE
                     ITGMEGETITMQDVFIYERKGVDKDGRVIGRFRPTGVRPRFAERLKIYGMQLPRSFFE
                     EVA"
BASE COUNT          528 a          657 c          657 g          485 t
ORIGIN      
        1 gcgtcggcca ggccccaggt gatacgcagt ttgcgcgcgg cgatcagacg gttgaggatg
       61 agacgtggca gaatgtaacc agcgccaaat gcaaacagcc ccggcatcag cgcaccgttc
      121 agcggccata ccagaaacgt gtacaccaac aataccagga gcggacacac cagcatcgat
      181 agaaactggg aggcccgata caccgacggt gcgtgaggcg aacgaaatcc cgcttgcatc
      241 aggtcttttt gcagcttttt agcgtttgcc gcggacggtg gcaccagacg gttgatcggt
      301 tcggcaagtc gctcggcgag agacgccaca gagttcggtt cgatacttgc ggcgacactt
      361 ccctgagcgt cgcccaattc cttcagccgc gctgccgtcg cgctgacagg acggaacatc
      421 agccaataga tcgccatcac accgaaagcg atgcagataa aagtggaaac cgcgattatt
      481 aacatatttt caccacctcg tttgtatcga cacgcccgtt agatcttgat cttcatgatc
      541 cgttggacca tcaacatgcc caggatctgc atggttacgg caaccgcgat cagcttctgc
      601 ccgcgcggat cgcgccagag cacatccatg taaccggggt tcatcacgga aatgtagata
      661 gccaggagaa tcggcagcgc gcacagcagc cacgccgacc agcgcgaact gagcgtcagc
      721 gtctttagat cttcgagaat gcggaagcgc tcacgaatcg tatacgccac tttttcgagt
      781 agctctgaga gattgccgcc cgtctcgcgt tggatcagga tcgccgtcac gcacatccgc
      841 aaatccagca gcggcatgcg ttggaccatg ttttcgagag ccagtttcaa cgacaacccg
      901 aggttctgct cctcgtaagt ctttcggaac tccgtcgcaa tcggttccgg catttccgta
      961 gacaccatgt gcagcgattc ggtaaacgcg tgtccggcag ttagtccgcg gctcatcagg
     1021 tcaagcgcat cgggcagcag ttgcagaaac ttgttgagcc gcttcttcct tttcgcgagg
     1081 acgtgtgagt acggcaaaat ggccgcgatt acgccgcaga gcgtcatcaa caggtaagaa
     1141 ctgctgacca tcgaaacggc gagaaacgcc agaacgccgg ccgttaacga gaagagcacc
     1201 aaacgtacaa ccgtgatgtt gaggtccgct tgatcgatgg ttcgtttgat acggcccggt
     1261 atagcgagtt tcagcagcga acgattgagc cacggtattt cgctcagtag ttcttcacgt
     1321 gcgagttgaa cgtccatatc ggcggaattg accgacgatc ggatcgcctc tgcgagtcgc
     1381 tcatttaata gcgccctctt cttatctgag gaacgcgaag tgatcaggta cagcgcgtat
     1441 ttcagaaaaa gacaggcgat gaaaacgaag aagggaatga gaagcattac gcaacctcct
     1501 cgaagaatga tctcggcaac tgcatgccgt aaatcttgag tcgttctgcg aagcgtggtc
     1561 gcacgccagt gggccggaac ctgccgatca ctcgcccatc cttatcgaca cctttccgct
     1621 cgtagataaa gacgtcctgc atcgtgatcg tttcgccttc catgccggtg atctcggaga
     1681 tcgagatgat gcggcgcgtg ccgtcactca aacgcgctac ctggatcacc atgttgattg
     1741 ccgaagcaat ctgctgtcgc atggcgcggt ctgacaaacg cataccggtc atttggatca
     1801 tcgtttccaa acgagcgaga gcgtcgcgag aagtgttggc gtggatcgtc gtgagcgagc
     1861 cgtcgtgacc ggtgttcatg gcctgaagca tgtcgatggc ttcgccgccg cgtacctcgc
     1921 cgatgacgat gcggtcggga cgcatacgca gggcgtttct caccaggtct cgctggctta
     1981 cctcgccttt gccttcgatg ttgggaggac gcgtttccag ccgcacaacg tgaggctgct
     2041 gcatctgcaa ttcggccgag tcttcaatcg tcacgatgcg ctcatcggat ggaatgtagg
     2101 cagacattgc gttcagcagt gtcgttttac ctgcgccggt accccccgaa ataagaatgt
     2161 tcaaacgcgc ggtgacgcac atctcgaaca ttgaagccat gtcgggagtc agtgcctggt
     2221 tttccagcag gcgattcatc cgcagcggtt cggcgccgaa ccggcgaatg gagagcactg
     2281 gaccatccag cgagagtggc gggatgatcg cattgacgcg cgagccg
//
LOCUS       DASTEY010000546         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_984187, whole genome shotgun sequence.
ACCESSION   DASTEY010000546 DASTEY010000000
VERSION     DASTEY010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984187"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFO72_09565"
     CDS             <1..392
                     /locus_tag="VFO72_09565"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HET9479581.1"
                     /translation="LPVSSLVRPEQYQHALNKVNRNAVLIETPPHSTGLAFDIDYRYM
                     SAAEQTFVMDELARLKSEGRIEAIRERNANYHVFAFIDGTRPGDDLIAAKLDEAMPPG
                     REAHHAEKKSKPAGKPAKVRPKRKRRR"
     gene            586..1506
                     /locus_tag="VFO72_09570"
     CDS             586..1506
                     /locus_tag="VFO72_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET9479582.1"
                     /translation="MTNAKVHVALILAVLAAVVTFVSFTARSSSGQSSRTRRQASVKP
                     TARIAELNRSERAGATSAFMTAAAQNATLRSQLSWSFGGKQQRGWHLYDLLIGKTLDT
                     RDDTDTSDFASALAKWQKRSGLSADGVLDEDALMRMVSQWQSNRLKNRAYAEPGQLVT
                     APPTDFYDPGRAAELRQVERNTYDAYKRMVAAAIADPSLKLARTSSGELAPGEKYFKI
                     ISAFRSREYQDQLRRKSPNAGRAGLAVNSPHFTGRALDIYVGGDPVDTKDSNRAIQVN
                     TPAYRWLVHNAVRFGFRPYFYEPWHWEYVK"
     gene            1665..>2408
                     /locus_tag="VFO72_09575"
     CDS             1665..>2408
                     /locus_tag="VFO72_09575"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET9479583.1"
                     /translation="MCSAPLRSQTKPDKEYLFYVLSEAADKIALVRFGPDGAKVERQI
                     DTGDMPIDIDGPHGIVVSPDRQFYYVSIAHGRPFGTVWKYATKDDSIVGKTTLGHFPA
                     TMDITPDGHFLYVVNFNLHGDMAPSTVSVVSTQTMTEVARIPTCTMPHGSRLNPQGTK
                     QYSACMMDDMLVEIDTRSLKATRHFLVTKNKEGGMAGPPAVHAPSHAVHGTKNTGGHG
                     SEAPKPGDNSCSPTWAEPSADGSSVFVACN"
BASE COUNT          547 a          702 c          674 g          485 t
ORIGIN      
        1 cactgcctgt cagctcactg gtcaggcccg aacagtatca acacgcactg aacaaagtaa
       61 atcgcaatgc ggtgttgatc gagacaccac cacattcaac gggtctcgcg tttgacattg
      121 attatcgcta catgagcgcc gcggagcaga cgtttgtcat ggacgaactc gcgcgtctga
      181 agagtgaggg acgcatcgaa gccatacgcg aacgtaacgc caactatcac gtattcgcgt
      241 tcattgatgg tacacgtcct ggcgacgacc tgattgccgc gaaactggac gaggcgatgc
      301 cgcccggacg cgaagcacat cacgcggaaa agaagagtaa accggcgggc aagccagcga
      361 aagtccggcc caagcgcaag cgacgtcgtt aatggagaat gtgcagtttg agaaactctt
      421 acctgggacc gcaggcgttt cgcctgcctc tttgaatagg ggtgctgctg agagtttgcg
      481 ggcgagacgc ctgcggtccc aggttgaacg aaccgtatgc tattctcgtg catcccaacg
      541 tttcgcccgg caacaacctc acgtaaaatg gcggtacctg aagacatgac taatgccaaa
      601 gttcatgttg cgttgattct ggctgtgctc gcggcggtgg tgacgttcgt ttctttcacc
      661 gctcgtagca gcagtggcca atcctcgagg acaagacgcc aggcgtccgt caaaccgacc
      721 gcacgcatcg ccgagttgaa tcggtcagaa agagccggcg caacgtccgc tttcatgacg
      781 gcggctgcgc agaacgccac gctgcggagt caattgagtt ggtcgtttgg cggtaaacag
      841 caacgtggct ggcatctcta tgacttactg atcggtaaaa cgctcgacac gagggacgac
      901 actgatacga gtgatttcgc aagcgcgctg gcgaagtggc agaagcgatc gggcctgagc
      961 gcggacggtg ttcttgatga agacgcactt atgcgcatgg tttcgcagtg gcaaagtaat
     1021 cgactgaaga accgtgcgta tgctgagcca ggccaactgg tcacagcgcc tccaacagat
     1081 ttctacgatc ccggccgcgc cgcagaactg cgtcaggtcg agcgcaacac ctacgacgct
     1141 tacaagcgaa tggttgccgc agcgattgct gatccgtcac tgaaactcgc gcgtacttca
     1201 tcgggcgaac tcgcaccggg cgagaagtat ttcaagatca tctccgcgtt tcgttcgcgc
     1261 gaataccagg accaactgcg ccgaaaatca cccaatgccg gccgagcggg attagcagtc
     1321 aacagtccgc actttacggg ccgcgcattg gacatctatg tcggcggcga tccggttgac
     1381 accaaagatt cgaatcgcgc aattcaggtc aacactccgg cttatcgctg gctggtccat
     1441 aacgccgtgc gcttcggctt ccgtccttac ttctacgagc cctggcattg ggagtacgtg
     1501 aagtagatcc aacatctgta atacactcgt gcgtcatggc acggcagcag tttgaacctg
     1561 ggaccgtggg cgtctcgccc gcaacttacg taagcgtcca acttgtccca gtgcaggcga
     1621 gacgcctgcg gtcccaggtt tttgcagtac tactgcttct aatcgtgtgc tcggcgccac
     1681 tgcgctcgca aaccaagccg gacaaagaat atctcttcta cgtcctgtca gaagccgcgg
     1741 acaagatcgc attggtccgc tttggtccag acggcgctaa agtcgagcgc cagatcgaca
     1801 ccggcgacat gccgatcgat atcgacggcc cgcacggcat cgttgtttca cccgaccggc
     1861 agttctacta cgtttcgatc gcgcatggcc gtccgttcgg caccgtctgg aagtatgcga
     1921 ctaaagacga ctccatcgtc ggtaaaacca cgctcggcca tttcccggcc acgatggata
     1981 tcacgcctga tgggcatttt ctttatgtgg tgaacttcaa cctgcacggc gatatggcgc
     2041 cgtccacagt ttcagtcgtg tcgacccaga caatgaccga agtcgcgcgc attcccacct
     2101 gcacgatgcc gcacggttcg cggctcaatc cgcagggcac gaaacaatac tcagcctgca
     2161 tgatggacga catgctggta gagatcgata cgcgttcgtt gaaggcgacg cgccactttc
     2221 tcgttacaaa aaacaaagag ggaggaatgg caggcccgcc ggcagtgcat gctccttcgc
     2281 acgcagtgca cggcacgaaa aacactggcg gacacggctc tgaggcgccg aagcccggcg
     2341 acaactcgtg ttctccaacc tgggccgagc cttcagcgga tggctcgtca gtttttgtag
     2401 cttgcaac
//
LOCUS       DASTEY010000547         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_671386, whole genome shotgun sequence.
ACCESSION   DASTEY010000547 DASTEY010000000
VERSION     DASTEY010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671386"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFO72_09580"
     CDS             <1..286
                     /locus_tag="VFO72_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET9479584.1"
                     /translation="VPLLDGAMELATAGLLTSGDKTNREYIGADIEIAPSVDSNLVKL
                     FFDPQTAGGMLIAAAEDKAEALLRTLRDNYRQAQILGRVSPRGSHSIVVS"
     gene            309..1592
                     /gene="egtB"
                     /locus_tag="VFO72_09585"
     CDS             309..1592
                     /gene="egtB"
                     /locus_tag="VFO72_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET9479585.1"
                     /translation="MTIVDLYQRVRARTMEIVAPLEIEDYVIQTAEFMSPPRWHIGHT
                     SWFFETVLQAHKPGYKVYSEDFLFYFNSYYEGFGPRIERPKRGTKSRPTARETVAYRK
                     HIDEQMLDFLRLGESVESVSSVVRLGLEHEMQHQELLVYDIKHLLCDQFQPRMMPPPK
                     SQFKVEGMAEIEGGLFWLGFDGKGFSFDNEKPAHQVFLRDFQIDKRLVTNDEYLEFIR
                     DGGYQNFRWWFSEGLETVKRENWQAPLYWELHDGEWLIRDFSGLQPASSRGQDPVSHV
                     SYYEASAFAKWAGKRLPTEAEWEKAACYDAERKVRQRFPWGDTDPDSGNTNLLENGFW
                     SVTPAGAFPEGANAHGCQQMIGDVWEWTTSDYVPYPGFKTEFDEYNDKWFVNQKVLRG
                     GSFATPQLHIRTTYRNFFHAHERWMISGFRCARDV"
     gene            1625..>3981
                     /locus_tag="VFO72_09590"
     CDS             1625..>3981
                     /locus_tag="VFO72_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET9479586.1"
                     /translation="MSPRSALKFVFVVSVLAVLAAPISAQNNSVNPRYTTPKSPNDTA
                     YGEKISEYTTEKYFMTELVDHLPLSDKVPSPDKILGYVIGTPNKLTYTKDLHRYYRAL
                     AGASSRVRVLVAPEKSEEGREQLLVLVSDEANLARLDRYKEITAKLADPRKLSEAEAQ
                     TLIAEGKPFYWASGSIHSPETGSPEMLMELAYRLAVEDSPFIEDIRKNMIVMITPTLE
                     VDGRDRMVDLYYYRKANPGKPAPPLLYWGKYVAHDNNRDSLGMALALSRNQMKTFLEY
                     HPTVLHDLHESVPYLYTMTGTGPYNAWLDPLALDEFQVLAYHEVEEMTKRGVPGVWTH
                     GFYDGWAPNYMLYIATGHNSIGRFYETFGNGGADTRDRTLGANQTSRVWYRPNPPFPR
                     VKWSLRNNVNMQQSALLFAMNFVAKEKERFLNNFYLKSKRAVAKATNEGPAAYVIPGD
                     TPRPVEAADMVNLMRLQGIEVHRATKDFTVKDQKYPGGSYIIRMDQPYSRWADMMLDT
                     QYYNVNDPAPYDDTGWTMGAMRNVKTVRVVDKSVLGVEATLLTADVKVTGQLTGPASA
                     VAYVINHNTENTLATLRFRLKDVKMNAAEDSFKIGERQFNTGSFIISRDGNPLNLRQQ
                     LESAVAELGLKAVGVEKLPDVKTHELAVPRIAIVHTWTNTQNEGWYRIEFDRLQIPYA
                     YISDQTIRNTPNLREKYDVLIFPPMGGSAQTIVNGMPMRGDPIPWKASALTPNFGLSP
                     DQTDDMRGGMGVAGVANLQKFIHEGGLFISIGNSVSSIPIDYGIT"
BASE COUNT          929 a         1137 c         1096 g          819 t
ORIGIN      
        1 cgtgccgctg ctcgatggcg cgatggaatt ggcaacggca ggcctgttga ccagcggcga
       61 caagacaaac cgggagtaca tcggtgcgga tatcgagatc gcgccatccg tcgattcgaa
      121 tcttgttaag ctgtttttcg atccccagac cgccggcggg atgttgatcg ctgccgcgga
      181 agacaaagct gaggcgttgc tgcgcacttt gcgcgacaac taccggcagg cgcagatact
      241 tggacgagtt tccccgcgcg gctcacactc cattgtcgtg agctagtatt attgtcccca
      301 ataaaatcat gacgatagtc gatttgtacc agcgcgttcg tgcgcgcacg atggaaattg
      361 ttgcgccgct cgagattgaa gactacgtga ttcagacggc ggagttcatg agtccgccgc
      421 gctggcacat cggccacacg tcgtggttct tcgaaacagt gctccaggcc cacaagcctg
      481 gttacaaggt ctactccgaa gactttctgt tttatttcaa ttcctactac gaaggtttcg
      541 gcccacgaat cgaacgacct aaacgcggga cgaagtcacg tcccacggcg cgcgagacgg
      601 tcgcctatcg caagcacatc gacgagcaga tgctcgactt tctccgcttg ggtgagtcgg
      661 ttgaatccgt gtcgtccgtg gtccgtttgg gtcttgagca cgaaatgcag catcaggagt
      721 tgctggttta cgacattaaa cacttgttgt gtgatcagtt tcagccacga atgatgccac
      781 ctccaaagtc gcagtttaaa gttgaaggga tggctgaaat cgaaggcggc ctcttttggt
      841 taggttttga cgggaaggga ttttcgttcg acaacgagaa gccagcgcat caggtcttcc
      901 taagggattt tcagatcgat aaacgactag tgaccaacga cgaatacctc gaatttattc
      961 gcgacggtgg ttaccaaaac tttcgctggt ggttctccga aggcttggaa acagtgaagc
     1021 gcgaaaactg gcaagcgccg ctttattggg aactgcacga cggcgagtgg cttatacgcg
     1081 acttctccgg ccttcagcca gccagcagca gaggccagga tcccgtttct cacgtcagtt
     1141 attacgaagc ttccgccttc gcgaagtggg ccggcaaacg actccccaca gaagctgaat
     1201 gggagaaggc ggcgtgttat gatgcggagc gcaaagttcg acaacgattt ccctggggcg
     1261 acaccgatcc cgatagcggc aacaccaatc tactcgaaaa cggtttctgg tcggtgacgc
     1321 ctgccggtgc gtttcccgaa ggcgccaacg ctcacggctg tcagcaaatg attggcgacg
     1381 tttgggagtg gacgacctcc gattacgtac cttacccggg attcaaaact gaatttgacg
     1441 agtacaacga caagtggttc gtcaatcaga aggttttgcg cggcggctct tttgccacgc
     1501 cccagttgca catcagaacg acgtacagaa acttcttcca cgcgcacgaa cgctggatga
     1561 tctcaggctt tcgctgtgcg cgtgacgtct agagccacag attacaccga ttcaggagcc
     1621 catcatgtcc ccaagatccg cattaaagtt cgtgttcgtg gtgtcggtgc ttgccgtttt
     1681 agctgcgccg atatccgctc aaaataactc cgtcaaccca cgctacacca cgccaaagtc
     1741 gccgaacgac acggcctacg gcgagaagat ctccgagtac accaccgaga agtacttcat
     1801 gaccgagctg gtggaccacc tgcctctgtc tgacaaggtg ccctcgcctg acaagattct
     1861 gggctacgtg atcggcacgc caaataaact gacctacaca aaagaccttc accgctacta
     1921 ccgcgcactc gcgggtgctt cgtcacgcgt tcgcgtgctg gttgcgccgg agaaaagtga
     1981 agagggacgc gaacagttgc tggtgctggt gagtgacgaa gccaatctgg ccaggttgga
     2041 tcgctacaaa gagatcaccg ccaaacttgc cgacccgcgc aagcttagcg aagcagaggc
     2101 ccagacgctg attgccgaag gcaaaccgtt ctactgggct tcgggttcga ttcattcgcc
     2161 ggaaaccggg tcgccggaaa tgttgatgga attggcatac cggctcgcag tcgaagactc
     2221 gccgttcatc gaagacattc gcaaaaacat gatcgtgatg atcacgccca cgctggaagt
     2281 cgacggtcgc gatcggatgg tcgatctcta ctactatcgc aaggcaaatc cgggcaaacc
     2341 ggcgccgccg ctgctttatt ggggcaagta cgtcgctcac gacaacaacc gcgatagtct
     2401 cggcatggcg ctggcgctct cacgcaatca gatgaagaca ttcctcgagt accacccgac
     2461 cgtgcttcac gatctgcacg agtcagtgcc gtatctttac acgatgaccg gcacgggccc
     2521 gtacaacgcc tggctcgatc cgctggcgct cgatgagttt caggtcttgg cctatcacga
     2581 agtcgaggag atgacgaagc gcggcgtacc tggcgtttgg acgcatggct tttacgatgg
     2641 ttgggcgccg aactacatgc tctacatcgc caccggacac aactccatcg gccgctttta
     2701 cgaaactttc ggcaatggtg gcgccgacac gcgcgaccgc acgctcggcg ctaatcagac
     2761 ttcacgcgtt tggtaccgtc cgaatccgcc gttcccgcgt gttaagtggt cgctacgcaa
     2821 caacgtcaac atgcagcagt ccgcactgct cttcgcgatg aacttcgtgg cgaaggaaaa
     2881 ggaacggttt ctaaacaact tctacctcaa gagcaaacgc gcggttgcca aggcgaccaa
     2941 cgaaggcccg gcagcctacg tcattccggg cgatactcca cgtcccgttg aagcagccga
     3001 catggtgaac ctcatgcggt tgcagggaat tgaagttcac cgcgccacga aggatttcac
     3061 tgtcaaggat cagaaatatc cgggcggttc ctacatcatt cgcatggatc agccgtattc
     3121 gcgctgggct gacatgatgc tcgacacaca gtattacaac gtcaacgatc ccgcgccata
     3181 cgacgacacc ggctggacta tgggagctat gcgcaatgta aagaccgtgc gcgtagtcga
     3241 caagagcgtg ctcggcgttg aggccacgtt gctcaccgct gatgtgaagg tcacggggca
     3301 actcacgggt ccagcgtccg cagtcgctta cgtaattaac cacaacaccg agaacacgct
     3361 cgccacgctt cgttttagat tgaaagacgt gaagatgaat gccgctgaag acagttttaa
     3421 aatcggcgag cggcagttta ataccggttc gtttatcatt tcgcgtgacg gaaatccgtt
     3481 aaatctacgg caacagctcg agtcagcagt ggccgaactc ggcctgaaag ccgtcggcgt
     3541 cgaaaaactg cctgacgtga agacgcacga gctcgctgtg ccgcgtatcg cgatcgttca
     3601 cacctggaca aacacgcaga acgaaggctg gtaccggatc gagttcgacc gattgcagat
     3661 tccgtacgcg tacatttccg accaaacgat ccgcaacacc ccaaacctgc gcgaaaaata
     3721 cgacgtgctg atcttcccgc cgatgggcgg ctcggcgcag acgatcgtga acggcatgcc
     3781 gatgcgcggc gatccgatac cgtggaaagc ttcagcgctc acgccgaact tcgggctgtc
     3841 gcccgatcaa acggacgaca tgcgcggcgg catgggcgtg gccggcgtag caaaccttca
     3901 gaagttcatc cacgaaggcg gactcttcat cagcatcggc aacagcgtct cgagcattcc
     3961 gatcgactac ggcatcacca g
//
LOCUS       DASTEY010000548         6183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1627167, whole genome shotgun sequence.
ACCESSION   DASTEY010000548 DASTEY010000000
VERSION     DASTEY010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627167"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFO72_09595"
     CDS             complement(<1..654)
                     /locus_tag="VFO72_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9479587.1"
                     /translation="MNDGSKTLIFAADLGGTHLRAATVDARGKIHFRFKQNTPQGTDP
                     DKIVAAVVSAVQECRKQFGDAADELKAVSVVVPGTVNVEQGIVVKAPNLPCLDGFPLT
                     AALAEELGLPATLENDANAAAVGEMWQGAARGCRTIICVTLGTGVGGGIILDGELWRG
                     VDGAAAEIGHMCVDPFGGVACTCGSRGCLEVFASATAIVRMTREASPRYPDSVLHASS
                     "
     gene            complement(651..3779)
                     /locus_tag="VFO72_09600"
     CDS             complement(651..3779)
                     /locus_tag="VFO72_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9479588.1"
                     /translation="MLKAVVILILLLSSSAALAQPRNVPDWVRDGVIYEIYPRAFSQQ
                     GNFNAITDRLDELKDLGVTILWLMPIHPIGQEKKKGTIGSPYAVRDYYAINPDYGTAN
                     DLKRLVREAHARGLKVIIDVVANHTSWDSVLMKHPEFYKRDAKGNITYPYDWYDIAAL
                     NYNNQQLRRYMIDMLKYWIREFNLDGFRCDVAGEVPTDFWETARAELEKVKPEILMLA
                     EAHKAELLVKAFDLDYSWPLHSALSKVLQGLAPASELRVEWQKEVEQWPKGALHMRFS
                     DNHDERRAIARFGERAALAASAFVFTLDGVPMIYNGMEVGDTTESGAPALFEKLPIFW
                     PIGERRPEFRRTYKQLMSMRRGSKALRRGTLEWVENSDELRVVSFVRRADGEEVLVAI
                     NFSSVPFVGTVGSSAVRLDPWQYMVMTNSVGNLVWRNPDARWPTAAKNGFGTSVTLGS
                     KVWFTLTNGVMTEVFYPTVDVPNVETLQLIVLTDERTELETHDTIHRLELPSPGSLTF
                     RQVNTAKSGQYTITKTYVTDPQRNTVLIDVEFDSRVPAGLSVYYDPSLYNSGKYDLGW
                     TEGNALVAVDGNIASALVSNCGLESHADLVSQTENTNVVQIGTLKRPEPHRPAKLQCT
                     IALGFGESVASATNAARGSLARGFAAIRSEYEAGWRRYTSGLPRVDAQHQRQFNMAAM
                     VLRALEDKTFRGAVIASPSSPWGGGPNANEPTVSGYHAVWARDLYHVATAFIALGEFE
                     TADRLLNYLFFVQQKSDGSFPRNSWVDGRPTGDGLQMDQVALPIVLAYQLKRTDADTW
                     QSHVKPAADFIVRRGPRTDQDRWEEKSGYFPGTIAAEIAGLVCAAEIARINGDTTSAA
                     TYLKTADDWARNIQLVDPARVDAGFLELVRLGIKAARDRSIVDAVALVDETLRVRTPA
                     GEAWYRYNNDTYGETPTGGDFDGRNGVGRLWTLLTGERGQYEIAAGDLAAARKRLDTM
                     AQFANDGLMIPEQVWDRQESPNAAFRFGAGTGSATPLAWSMAQFIRLAINLKLGRNLE
                     TPDIVAQRYLSK"
     gene            complement(3772..5091)
                     /locus_tag="VFO72_09605"
     CDS             complement(3772..5091)
                     /locus_tag="VFO72_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9479589.1"
                     /translation="MQKPRLSFWQIWNMSFGFLGIQFGWGLQLANMSPIYRYLHAEES
                     QLPYLWLAGPVTGLLIQPIIGSMSDRTWNRLGRRRPYFLVGAILASVSLFVMPDSSAL
                     WMAAGLLWILDASINITMEPFRAFVADKLPEDQRTLGFVMQSWFIGIGQTFANALPYL
                     FSALGVVGVMATGIPLSVDYAFKIGAIVFLVAVLWTVLTTKEYPPEDMEAFARLRNEK
                     RGCLGGFAEIASSLAEMPTTMKQLAVVQFFTWFALPCMWQFFGIATARHVFGAPDETS
                     PLFAEGVEWAGLCFAVYNVVCFVVAFMLPWLASETSRKTVHMIALLCGGVGLISVMFI
                     SNKWMLFASMAGVGVAWASILAMPYVMLSTAVPPARMGVYMGVFNLFIVIPQIVMSLI
                     VPRIFNNVLGGDPRNAVVLGGISFLIAAASVVIVRDVHEARSQEIRA"
     gene            complement(5091..>6183)
                     /locus_tag="VFO72_09610"
     CDS             complement(5091..>6183)
                     /locus_tag="VFO72_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9479590.1"
                     /translation="IKIIQDQVVNHTGPYHPWVDDPPTPTWFNGTKTNHLKNVFQTWV
                     LHDPRPVRQLRRETMEGWFLDFLPDLNQFDYEVSRYLIQNTLWWIGITGLDGIRMDTW
                     QYVPNEFWRDWTARVKREFPNFKVVGEVKDGDVVHTSFFQGGRVRFDGIDSGLDSLLD
                     FPLFYSIRHAFAEGKSIDEIPKTLARDYLYTNPDILVTMLGGHDDGRFMSEKGATIAG
                     LKLANAFVLTTRGVPQLYYGDEIGMTGPDEPTTREDFPGGPRTKEERELFEYIRALIR
                     LRHELAPLRSGRLINLYVSEQQYAYARGDVIVVINNDDKQAEISFHTDLTDGKVLHDR
                     LGVSSRISVKDGMLKVKLPERSVAVFVED"
BASE COUNT         1389 a         1884 c         1626 g         1284 t
ORIGIN      
        1 gctgctcgcg tgtaaaactg aatcaggata acgaggactc gcctcgcgcg tcatgcgcac
       61 gatggccgtt gcggacgcaa acacctccaa acaaccacga ctgccgcacg tgcacgcgac
      121 cccgccaaac ggatcaacgc acatgtgccc gatctccgcc gccgcaccgt caacgccgcg
      181 ccacaactcg ccgtcgagaa ttatcccacc accgactccg gtgccgaggg tcacgcagat
      241 gattgttcgg cacccacgag ccgcgccctg ccacatctca ccgaccgccg ccgcgttcgc
      301 atcgttttcg agagtcgccg gcagaccgag ctcttccgcc aacgcggcgg tgagcggaaa
      361 gccgtcgaga cacggaaggt tcggcgcttt cacgacaatt ccctgctcga cgttcactgt
      421 tcccggcacc accaccgaca cggctttcag ttcgtcagca gcatcgccaa actgtttgcg
      481 gcattcctgc accgcgctga cgacagcggc gactatctta tccggatcag ttccctgcgg
      541 cgtgttttgc ttgaaacgaa aatggatctt gccgcgtgcg tccacggttg cggcgcgcag
      601 gtgcgtgccg cctagatcgg ccgcgaagat cagggttttg gatccgtcgt tcatttcgag
      661 agatagcgct gagcgactat gtcgggtgtc tcgaggttgc gaccgagttt caggtttatc
      721 gccaagcgga tgaactgcgc catggaccac gccaatggag ttgccgatcc ggtgcccgcg
      781 ccaaaacgaa atgccgcgtt tggcgattct tgtcgatccc aaacttgttc cggaatcatg
      841 aggccgtcgt tggcaaactg cgccatcgtg tcgagccgtt tacgcgcggc ggcgagatca
      901 cctgcggcaa tttcgtattg accacgttca ccagtgagca acgtccaaag gcgtccaacg
      961 ccgttgcggc catcgaagtc gccgccggtc ggcgtttcgc cgtaagtgtc attgttgtag
     1021 cgataccacg cctcgcctgc gggagttctt accctgaggg tctcatcaac cagcgccacc
     1081 gcatccacaa tcgaccgatc cctggccgct ttgattccca gacggaccaa ctccaaaaac
     1141 ccagcgtcaa ctcgggctgg atctactagc tgaatgtttc gcgcccagtc gtcagcagtt
     1201 ttaagatagg tagcggcgga agtggtatcg ccgttgatcc tcgcaatttc ggccgcacaa
     1261 acaaggcccg cgatttccgc ggcaatagta cccgggaaat accctgactt ttcttcccag
     1321 cgatcctggt cagttcgcgg cccgcgccgc acgataaagt ccgcagcggg cttgacgtga
     1381 ctttgccagg tgtcggcgtc ggtgcgtttg agttgataag ccagaactat tggcagtgcg
     1441 acctggtcca tctgcaagcc gtcgccggtc ggacgcccgt cgacccaact gtttcgcgga
     1501 aaactgccgt cgcttttctg ttgcacgaaa aagaggtagt tcaacaaacg atccgccgtc
     1561 tcgaattcgc cgagcgcgat gaaggcggtc gctacgtgat acagatcgcg cgcccaaaca
     1621 gcgtgatagc cgctgacggt cggttcgttc gcgtttggac caccgcccca cggcgacgaa
     1681 ggcgaagcaa tcaccgcgcc gcgaaacgtc ttgtcttcaa gtgcacgcaa caccatcgca
     1741 gccatgttga actgccgctg gtgctgtgca tccacgcgcg gcaaaccgga cgtgtagcgg
     1801 cgccagcctg cttcgtactc actacggatc gccgcaaacc ctctcgcgag cgatccacgg
     1861 gcagcattcg tagcactcgc aacgctttca ccaaatccga gcgcgatcgt gcattgcaac
     1921 ttcgccggcc tgtgcggttc aggacgcttt agggttccaa tctggacgac gttagtattt
     1981 tcggtttggc taacgaggtc cgcgtgcgac tccaagccgc agttcgacac cagcgctgac
     2041 gctatattgc catccactgc aaccaacgca ttgccttcag tccatcccaa gtcgtatttg
     2101 ccgctattgt agagagacgg atcgtagtaa acggaaagtc cggccggaac ccgactatca
     2161 aactccacgt caatgagcac ggtgttgcgt tgcggatcgg tgacgtaggt tttcgtgatc
     2221 gtgtactgac cgctctttgc agtgttgact tgccggaatg tcagcgaacc gggactcggc
     2281 agttccaggc gatggatagt gtcgtgagtc tcgagctcgg ttcgctcatc ggtgagcacg
     2341 atcagctgca atgtctcgac attcggcaca tcgactgttg gataaaacac ctccgtcatc
     2401 acgccgttcg tcaatgtgaa ccacactttc gaaccgagtg tcacagatgt gccgaaacca
     2461 ttctttgccg cggtaggcca gcgggcgtcc ggatttctcc acacgaggtt accgactgaa
     2521 ttggtcataa ccatgtattg ccacggatcc agtctaacgg ctgacgatcc aacggttccc
     2581 acgaaaggca cagacgaaaa gttgatcgct accaacacct cttcgccgtc agcacgcctg
     2641 acaaacgaca cgacgcgcaa ttcgtcagaa ttctctaccc actcgagcgt gccgcgcctc
     2701 aatgccttgc taccgcgcct catcgacatg agttgtttgt aagtccggcg aaactcgggc
     2761 cgccgctcgc caatgggcca aaagatcggc aacttctcaa acaacgcggg tgcgccggat
     2821 tcagtcgtat cgccgacctc catgccgttg tagatcatcg gcacaccatc gagcgtgaac
     2881 acaaacgccg acgccgcgag cgccgcccgt tcgccaaaac gcgcaatcgc acgccgttcg
     2941 tcgtgattgt ccgagaagcg catgtgcaac gcgcctttcg gccactgctc aacttctttc
     3001 tgccactcga cacgcaactc ggaagctggt gcaagacctt gcaacacctt gctcagcgcg
     3061 ctgtgcagcg gccatgaata gtcgagatcg aacgccttca ccagcaactc cgctttgtgc
     3121 gcttcggcaa gcataaggat ctcgggcttg accttctcca actccgcgcg cgcggtttcc
     3181 cagaaatccg tcggcacttc accagccacg tcgcagcgaa agccgtcgag attgaactca
     3241 cgaatccagt acttcagcat atcgatcatg tagcgccgca gttgctggtt gttgtagttc
     3301 aaggcggcaa tgtcatacca atcgtacggg tacgtgatgt tgcctttagc gtcgcgtttg
     3361 tagaactcgg gatgcttcat cagcacgctg tcccacgacg tgtgatttgc gacgacgtcg
     3421 atgatgactt tcaggccccg agcgtgtgct tcccggacga gccgcttgag atcattagca
     3481 gtgccgtagt cgggattaat tgcgtagtaa tcgcgtacag cgtaagggct gccgatagtg
     3541 cctttcttct tctcctgacc gatcgggtga atcggcatca gccagagaat tgtcacgccg
     3601 agatccttca gttcatccag gcgatccgta atcgcgttga agttgccctg ttgcgagaaa
     3661 gctctgggat agatctcgta gataactccg tcacgaaccc agtcgggtac atttctcggt
     3721 tgggccagcg cggcgctgga cgagagtaaa agaatcagaa taaccacggc cttaagcacg
     3781 gatttcttga gaacgcgctt cgtgtacgtc gcggacgatc acgacgctcg ccgcagcgat
     3841 caggaaactg atgcctccaa gcaccactgc attgcgcggg tctccgccga gcacgttgtt
     3901 gaaaattctc ggcacgatta gtgacatcac gatctgtgga atcacgatga acagattgaa
     3961 tacgcccatg taaacaccca tgcgggcggg cggcaccgcc gtcgacaaca tcacatacgg
     4021 catcgccaga attgatgccc atgccacccc aacgcccgcc atgctcgcga acagcatcca
     4081 cttgttacta ataaacatca ccgagatcag tccgacgccg ccgcagagca aagcaatcat
     4141 atggaccgtc ttgcgactcg tttcgctggc cagccacggc aacatgaacg cgacaacaaa
     4201 acagaccacg ttgtagaccg cgaagcacaa gccggcccat tcgacgcctt ccgcaaacaa
     4261 cggcgaagtc tcatccggtg caccgaaaac gtgtcgcgcg gtcgcgattc cgaagaactg
     4321 ccacatacac ggcagcgcaa accaggtgaa gaactggacc accgcgagtt gcttcatcgt
     4381 cgttggcatt tcggcgagtg atgacgcgat ctccgcaaaa ccgccgaggc atcctctctt
     4441 ctcgtttcgc aagcgcgcga aagcttccat gtcctcaggc ggatactctt tcgtcgtcaa
     4501 cacggtccaa agaactgcta cgagaaagac gatggcgcca attttgaaag catagtcaac
     4561 ggaaagcgga attcccgttg ccatcacacc gacaacaccc agcgctgaga acaagtacgg
     4621 cagcgcattc gcgaacgttt ggccgatgcc gatgaaccag ctctgcatta cgaacccgag
     4681 cgtgcgctga tcttcaggca acttgtcggc gacaaacgca cgaaacggct ccatcgtgat
     4741 gttgatactc gcgtcgagaa tccagagcag gcccgcagcc atccaaagcg ccgacgaatc
     4801 aggcattacg aacaacgaaa cgctggcgag aatcgcgccg acgagaaagt aaggacggcg
     4861 gcggccaagc cggttccacg tgcggtcgct cattgaaccg atgatcggtt gaataaggag
     4921 accggttact ggaccagcga gccacagata cgggagctga ctttcttcgg catggaggta
     4981 cctgtagatc ggactcatgt tggcgagttg cagcccccag ccaaactgaa taccgaggaa
     5041 gccaaagctc atgttccaga tctgccagaa gctcaaacgt ggtttctgca tcagtcctcc
     5101 acaaatacag ccacagatct ttcaggcaac ttaaccttta gcattccatc cttaacacta
     5161 atcctgctgc tcactcccaa acggtcgtgt aacacctttc catccgttaa atccgtgtga
     5221 aaactgatct cagcctgctt atcgtcgttg tttataacga cgatcacgtc accgcgcgca
     5281 tacgcatact gctgctccga aacgtacaga ttaattagcc ggccactcct cagtggcgcg
     5341 agttcgtgtc gcaagcgaat tagggcacgg atgtattcaa acaactcccg ctcctctttc
     5401 gtgcgtggtc cacccggaaa atcctcacgt gtcgtcggct cgtccggtcc agtcatcccg
     5461 atctcgtcgc cgtaatagag ctgcggcacg ccgcgcgtag tcaacacaaa cgcatttgcc
     5521 agcttgagtc cggcgatggt cgcacctttt tcactcataa agcggccatc gtcgtgacca
     5581 ccgagcatag tgacgagaat gtcaggattg gtgtagagat agtctcgcgc caacgtcttc
     5641 ggaatttcgt cgatcgattt cccctcagca aacgcatgcc gtattgaata aaagagcggg
     5701 aaatcgagca gcgaatccag cccggagtct ataccatcaa aacgcacgcg cccgccctgg
     5761 aaaaatgaag tatggaccac gtcgccgtcc ttcacttcgc cgacgacttt gaaatttgga
     5821 aactcgcgct tcactcgcgc ggtccagtcg cgccagaact cgttcggcac gtattgccac
     5881 gtgtccatgc gaatgccgtc gagtccggtg attccaatcc accacaatgt gttctggatc
     5941 aaatagcgcg aaacctcgta gtcgaactgg ttgaggtcgg gcaggaagtc gagaaaccag
     6001 ccttccattg tttcgcgcct cagttgtctg accggacgcg gatcgtgaag cacccaggtt
     6061 tgaaaaacgt tcttcaggtg gtttgttttg gtgccgttga accaggtcgg agtcggagga
     6121 tcatcaaccc acggatgata cggaccggtg tgattgacga cctggtcctg gatgatcttg
     6181 att
//
LOCUS       DASTEY010000549         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1528713, whole genome shotgun sequence.
ACCESSION   DASTEY010000549 DASTEY010000000
VERSION     DASTEY010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528713"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFO72_09615"
     CDS             complement(<1..490)
                     /locus_tag="VFO72_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479591.1"
                     /translation="MSTQLFSEHLGQRIAIAGLVIYVAFAPHSVAASAIGVVIAGIGW
                     GIRSIRTRSLGLSRSKFDLVILLSLLWTGLSAVLSEEQDISLAKLKSTWVVFLFYLTR
                     AVLTRRSALVMVAVLILSGCTGAIYSAFDLLRGRGVVVESIAPQSPFQQIQLHPQDTI
                     WRV"
     gene            652..>2554
                     /locus_tag="VFO72_09620"
     CDS             652..>2554
                     /locus_tag="VFO72_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479592.1"
                     /translation="MAFDKAKVLKAAEKFLSQGKINAAIKEYRQIVDNDADDLTTLNM
                     LGDLYVRSGKKEEAVRCFERIAEHYSAQEFNLKAIAMYKKIERIRTRDPIIAYKLAEL
                     YATQGLVHDARAQYLVVADAYTRSGDNKKALDVLHKIADLDPNNTEIRLKLADGYVKE
                     NMRREAAEAFVQAANRLHQTGAHEQALDAYNKALQLVRDDREALRGMLETHIARGTAD
                     EAAEVLERAVEGKEDDQELVSMLARAHLEAEDPRGAERATSLLMAQDSANYTQYLPVT
                     RLYLKLGEVDETIRILGTIIERMLAGREEKELLQIVTQVLDRNPDHVAALRMLVRIHW
                     WQRDMDALRSSLERLAESAEASELVDEERYALTQLVRLAPDEQRYLDRLNLLGGLPEE
                     SPDDFSSQPEEEPSDVPEFETFAVVEDEIEQPVAQMTDEFETFQSNAATGTTFTDPTA
                     SFADLNEETEVASDVATEVAVEGGDDQQRVETMMRQELESVDFYIAQGYSDIAFDTLE
                     MLERQFGPHPEIQARRDKLAASGGRPVVEAPAVFEFGGVEETPVARAEEATTFDDDSG
                     YAALAGEPLEAPGQVPKPGPVASGIDAGLAELFEEFRAAEEGDSVREDFETHYNMGTA
                     YKEMDLIDEA"
BASE COUNT          614 a          696 c          749 g          495 t
ORIGIN      
        1 caacgcgcca tatcgtatct tgagggtgta gttgaatttg ttgaaatgga ctctgcggcg
       61 cgatcgactc gaccactacg cctcgcccgc gcagcaagtc aaaggcgctg taaatcgcac
      121 ccgtgcaccc ggacagaatc agcaccgcga ccatgactaa tgcggaacgc ctggtgagca
      181 ccgcgcgagt caggtaaaag aggaagacga cccacgtgga cttgagtttc gcgagactga
      241 tgtcctgttc ctcagacaag acagccgaga gtcccgtcca aagcagcgac agcaggataa
      301 ccagatcgaa cttcgatcga ctcaaaccca gacttcgcgt ccgaatcgac ctgattcccc
      361 agccgatgcc ggcgatcacc actccgatcg cactcgcagc caccgaatga ggcgcaaagg
      421 ccacgtaaat aactagtccc gcgatggcta ttcgctgacc caaatgttca ctaaatagct
      481 gcgttgacac ctttatttac gcatgttagc gtatctcgcg gctgttttgc cggagttgga
      541 aagtggagtt ttagacaagc gcttatcaga atggcattgt cccgctgccc gctgctacat
      601 taaaagccgc cgtcgccagt atttccgtat taatgatacc taagattcct gatggctttc
      661 gataaagcga aggttctaaa agccgctgag aaatttcttt ctcagggaaa gatcaacgca
      721 gcaatcaagg agtatcggca gatcgtcgat aacgacgctg acgatctcac gacattgaac
      781 atgctgggcg atctctacgt tcgttccggc aaaaaagaag aagccgtgcg ctgcttcgag
      841 cgcatcgccg aacactacag cgcgcaggaa ttcaacctca aagcaatcgc gatgtacaaa
      901 aagatcgagc gtatccgcac gcgcgatccg atcatcgcct acaaacttgc cgaactctac
      961 gccacccagg gactggtgca cgacgcacgc gcgcagtatc tcgttgtcgc cgatgcttac
     1021 acacgctccg gcgacaacaa aaaggcgctc gacgtactgc acaagatagc cgatctcgat
     1081 ccaaacaata ctgagattcg tctcaagctc gccgacggtt acgtcaaaga gaacatgcgg
     1141 cgcgaagctg ccgaagcatt tgtgcaggcg gccaatcgtc tgcaccaaac cggcgcacac
     1201 gaacaggctc tcgacgcgta caacaaagca ctgcaactcg ttcgcgacga tcgcgaagcc
     1261 ttgcgaggca tgctcgagac gcacatcgca cgcggcactg ctgatgaagc tgccgaagtg
     1321 ctcgaacgcg ccgttgaagg taaagaggac gatcaggaac tggtctcgat gctggcgcgg
     1381 gcacatctgg aggcagaaga cccgcgcggc gccgagcgag cgacttcgtt gttgatggcg
     1441 caggatagcg cgaactacac gcagtatttg ccggtcacgc gtttgtatct gaagctcggc
     1501 gaagttgacg agaccatacg catcctcggc actattatcg agcgcatgct cgcgggtcgc
     1561 gaagagaaag agttactcca aatcgtgacc caggtgctcg atcgcaatcc ggatcacgtg
     1621 gcggcgttgc gcatgctggt gcgcattcac tggtggcaac gcgatatgga cgcgttgcgt
     1681 tcgtcgctcg aacggctcgc tgaatcagcc gaggcgtcgg agctcgtcga cgaggagcgt
     1741 tacgcgttga cgcaactcgt gcggctcgca cctgacgagc agcgttatct cgatcgtctt
     1801 aatttgctgg gtggtttacc ggaagaatcg ccggacgatt tctcatcaca gccggaggaa
     1861 gagccgagcg acgtgccgga gtttgagacg ttcgctgtgg tggaagacga gatcgaacag
     1921 cccgtcgcgc agatgacaga cgagtttgaa acgtttcagt ctaacgccgc gaccggtacg
     1981 acgtttaccg atccaactgc gtcgtttgcc gatctgaacg aagaaacaga agtcgcttcg
     2041 gatgttgcaa cggaagtggc agtcgaaggc ggtgacgatc agcaacgcgt cgaaacgatg
     2101 atgcgtcagg agctggagag tgtcgatttt tacattgcgc agggttactc cgacatcgca
     2161 ttcgacacgc tcgagatgct cgaacggcag tttggcccgc atccggagat tcaggcacgg
     2221 cgcgacaaac ttgcggcctc cggtgggcgg cctgtggtcg aggcgcctgc ggtgtttgag
     2281 tttggtggtg ttgaagaaac gccggtagcg cgagctgagg aagcgacaac gttcgacgat
     2341 gacagtggct atgcggcgct tgcgggtgag ccgttggagg cacccgggca ggtgccgaag
     2401 ccggggccgg tggcaagtgg tatcgatgcg gggttggcgg agctgtttga agagtttcgt
     2461 gcggctgaag aaggcgactc ggtccgtgaa gatttcgaga cgcactacaa catgggcacc
     2521 gcttacaaag agatggacct cattgacgaa gcaa
//
LOCUS       DASTEY010000550         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_210116, whole genome shotgun sequence.
ACCESSION   DASTEY010000550 DASTEY010000000
VERSION     DASTEY010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210116"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1839
                     /locus_tag="VFO72_09625"
     CDS             214..1839
                     /locus_tag="VFO72_09625"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HET9479593.1"
                     /translation="MASIRTERITEHFGRKNTSVDLDAYGIKEPARVFWNLNTPQLYE
                     EIARRGEGVLSNHGVVLVDTGEHTGRSAKDKAIVREPGSEDKVFWGDVNRDFPQAKFN
                     ALKERMMAHAKGRDLFVQDTFVGADPHYRLPIRVVSELAWHSLFARTMFISNNDEAQA
                     HKPEFTIINFPSFQADPQRDGTRSPTFILMDFSQRLVLIGGTSYAGETKKSAFTIMNY
                     LLPQRDVMSMHCSANIGAEGDVAIFFGLSGTGKTTLSADPERRLIGDDEHGWSENGVF
                     NYEGGCYAKVIKLSAEAEPDIYRTTRMFGTILENVVYDDVTREIDLDDARKTENTRAS
                     YPLTSIENIVPEGRAGHPRNIIMLTADAFGVLPPVARLTPEQAMYHFLSGYTAKVAGT
                     EKGIKEPEATFSTCFGAPFMVLHPGVYADLLGKQMDRHQAACWLVNTGWSGGPYGVGH
                     RMKIAYTRAMIRAILDGSLAKTDTIPDPIFGVGIPVSCPEVPTEVLQPRNTWADKDAY
                     DRQARDLARRFNENFKKYEAGVSESVRAVAPKT"
     gene            complement(1891..2241)
                     /locus_tag="VFO72_09630"
     CDS             complement(1891..2241)
                     /locus_tag="VFO72_09630"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET9479594.1"
                     /translation="MSNESCLFCRIITGQIPADYVHQDERCVVIRDINPQAPMHVLVI
                     PRDHLESLDDAAQKDEPLLGHLLRIGARVANEHVNGEGGYRSVINTGAGAGQSVFHLH
                     VHVLAGRPLNWPPG"
     gene            complement(2263..>2950)
                     /locus_tag="VFO72_09635"
     CDS             complement(2263..>2950)
                     /locus_tag="VFO72_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9479595.1"
                     /translation="AGSDTIQIEGVDALGAAEHTIIPDRIETGTFIVAAALTRGQLEI
                     KDCRPDHLHSVIEQLRHAGVVIHELNPTTLEVRCPDGLVAGDVSTAPYPQFPTDMQAQ
                     YMVLMTQARGRSEIVETVFENRFMHASELQRMGAQIQIDGSTAIVDGPTPLTGARVIA
                     SDLRASASLVLAGLIAKGETLIDRVYHIDRGYEKIEAKLLAVGADIERVRAPVTTSQL
                     QEMQPIVEPV"
BASE COUNT          654 a          853 c          811 g          632 t
ORIGIN      
        1 ggattctgca ctctttgttg tcacccaacg ctcgccagac gaggttgaag cagtgcaaac
       61 cgcggccgat cgtatcgaac gcgccgctcg cgacctcgct gacgcactgc gcgaactggc
      121 ccagcaacgc agggccgctg aagaatctta atacaactgg accagcagac ggctcgatag
      181 catttccccg tcctgtttac ttggagataa ctgatggctt cgattcgcac tgaacgaatt
      241 actgaacatt ttggtagaaa aaatacttct gttgacttgg atgcgtacgg tatcaaagaa
      301 ccggctcgcg tcttttggaa cctcaacaca ccgcaactct acgaagagat cgcgcgccgc
      361 ggcgaaggtg tcctttcaaa tcacggcgtc gtattggttg atacaggcga acacaccgga
      421 cgttccgcca aagacaaagc aatcgtgcgc gagcccggaa gtgaagacaa agttttctgg
      481 ggcgacgtca acagagactt tccgcaagcg aaattcaacg ctttaaaaga gcgcatgatg
      541 gcccacgcga aaggccgaga tcttttcgtg caggatacgt ttgtaggcgc cgatccacat
      601 taccggctgc cgatccgcgt ggtgagcgaa ctcgcctggc actcgctctt cgcgcgcacg
      661 atgtttatta gtaacaacga tgaagcgcag gctcataaac ctgagttcac gattatcaac
      721 tttccgagct ttcaggcaga tccacaacgc gacggcacgc gctccccaac gttcattctg
      781 atggacttca gccagcggct ggttttaatc ggtggtactt cgtacgcggg cgaaacgaag
      841 aagagtgcgt ttacgatcat gaactacctt ctgccacagc gcgacgtgat gagcatgcac
      901 tgctcggcga acatcggcgc tgaaggcgac gtggcgatct tctttggtct atccggaacc
      961 ggtaaaacta cgctctccgc cgatcctgaa cgacgtttga tcggggatga cgaacacggt
     1021 tggtccgaga acggcgtctt caattacgaa ggcggctgtt acgcgaaagt gatcaagctg
     1081 tcggcggaag cagagcctga catctaccgc acgacgcgga tgtttggaac gattctcgag
     1141 aatgttgttt atgacgacgt aacgcgcgag atcgatcttg acgatgcgcg caagactgaa
     1201 aacacgcgcg cgtcgtatcc gctcaccagc attgagaaca tcgtccccga aggacgtgcc
     1261 ggccacccgc gaaacatcat tatgttgacc gcggatgcgt tcggcgtgct accacccgtg
     1321 gcgcgcctga ccccggagca ggcgatgtat cactttcttt ccggctacac ggcgaaagtt
     1381 gccggcaccg aaaaaggcat taaagaaccc gaagcgacgt tctcgacctg ctttggcgcg
     1441 ccgttcatgg tgctccatcc gggcgtttac gccgatctgc tcggcaagca gatggaccgg
     1501 catcaggcgg cgtgctggct ggtgaatacg ggatggagtg gcgggcctta cggcgtcggg
     1561 catcgcatga agattgccta cacgcgcgct atgattcgcg ctattctcga cggctcgtta
     1621 gcgaagaccg atacgatacc ggatccgatt tttggcgttg gaattccggt gtcgtgtcct
     1681 gaagttccca ctgaggtgct tcaacctcgc aacacgtggg ccgacaagga cgcctacgat
     1741 cgccaggcgc gagatcttgc acggcgtttc aacgagaact tcaagaagta cgaggcaggc
     1801 gtctccgaat cggttcgcgc cgtcgcgcca aaaacatagc cacagattta cacggactct
     1861 gtgtaatgtg tggctggctt gcttgtggtt ctaacctggt ggccagttta gcgggcgacc
     1921 ggcaagcacg tggacgtgca gatgaaaaac tgactgcccg gcgccggcgc ccgtattgat
     1981 caccgagcga taaccacctt cgccgttcac gtgctcgttt gcgactcgcg caccgatacg
     2041 aagcaagtgg cccagcaacg gttcatcctt ttgcgcagcg tcgtcgagcg attcaaggtg
     2101 gtctcgcggg atcaccaaca cgtgcatcgg cgcctgcggg ttgatgtcgc gaatcaccac
     2161 acaacgctcg tcctgatgca cataatccgc cggtatctga cccgtgatga tcctgcagaa
     2221 caaacaactc tcgtttgaca tacgtctcct taactaaaat cctcaaaccg gttcgacaat
     2281 tggctgcatt tcctgaagct gactcgtcgt tacgggagcc ctgactcgtt ctatgtctgc
     2341 gccgactgcc aacagtttcg cttcaatctt ctcgtagccg cggtcgatgt ggtaaacgcg
     2401 atcgatcaac gtctcgcctt tagctatcaa tccggccaac accagcgacg ccgaagcacg
     2461 caggtccgat gcgatcacgc gcgctccggt cagcggagtt ggtccatcaa caatcgcagt
     2521 gctgccatcg atctgaatct gcgcgcccat gcgttgcagt tccgaggcgt gcatgaagcg
     2581 attctcaaaa acggtctcaa cgatctcgga acgtccgcgc gcctgcgtca tcagcaccat
     2641 gtactgtgcc tgcatgtccg tcggaaactg cggatatggc gccgtggaaa cgtcacccgc
     2701 cacgagacca tcgggacacc ttacttccaa tgtcgttgga ttcagttcgt gaatcacgac
     2761 gccggcatgt cgcaactgtt cgatgacgct gtgcaaatgg tccggacggc agtcctttat
     2821 ctcgagctgc cctcgggtga gcgctgcagc gacgatgaac gttccagttt cgatgcggtc
     2881 gggaataatc gtgtgctccg ccgcgccaag cgcatcgact ccttcgattt gaatcgtgtc
     2941 tgatcccgcg
//
LOCUS       DASTEY010000551         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_12355, whole genome shotgun sequence.
ACCESSION   DASTEY010000551 DASTEY010000000
VERSION     DASTEY010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12355"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..800
                     /locus_tag="VFO72_09640"
     CDS             60..800
                     /locus_tag="VFO72_09640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9479596.1"
                     /translation="MEQRLQKLIATAGIASRRHAEELITAGRVTVNGEIVRELGTKAD
                     PERDHIKVNGKLINPQLKARDKVYVLLNKPRGYLSSVSDPEGRPLVTELLPASLGRLH
                     PVGRLDFNTEGLLLLTNDGDFTNYVTAARNRVEKVYEVKVKGVPSDGAIQRLRKGVRL
                     EDGTRTAPAKITNLGETQTNAWYEVLLHQGRNQQVRRMFELIGHSVLKLRRVRIGFLT
                     DDKLKPGEWRFLSPSEAGRLMKPQRHSQ"
     gene            936..2081
                     /locus_tag="VFO72_09645"
     CDS             936..2081
                     /locus_tag="VFO72_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9479597.1"
                     /translation="MHFELTEEQQQVKSSVREFAESEIAPHVLEWDESQHFPIELKPK
                     LAELGLMGVIFPEEYGGAGMGYVEYATIIEELSRVDGSVGISIAAHNSLCSNHIYKYG
                     TEEQRRKYLVPLASGEHLGAWGLTEPSAGSDASGTKSTAVRADGGWTVNGSKNFITHA
                     IHGDTCVAMAVTDRAKRSKGISAFIFEKGMKGFSPSKKENKLGLRASETASVVFEDCF
                     VPDNQLLGEEGQGFVNAMEILDGGRISIAALAVGIAQGAYESALRYAQERQQFGKPIA
                     EFQAIQFKLANMATQIDAARLLMYRAAWMKDQGRRTTKESSMAKLFASEISVEVCEEA
                     IQIHGGYGYTKDYPPEKYWRDSKLCTIGEGTSEIQRIIIARELLRQV"
     gene            2212..3150
                     /gene="meaB"
                     /locus_tag="VFO72_09650"
     CDS             2212..3150
                     /gene="meaB"
                     /locus_tag="VFO72_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9479598.1"
                     /translation="MTTKSLSERVRQGDAAAVARAISKIEDGSDGAAALMKEIYQLSR
                     GALVVGITGAPGAGKSSLVDKLALLYRQRGERVGIVAVDPSSPFSGGAILGDRIRMQA
                     LGLDEGVFIRSMATRGNLGGLARATVDAVAILDAAGYAKVIIETVGVGQDEVEIVKAA
                     DVSIVVLVPGMGDDIQAIKAGIMEIGDIFVINKADRDGVYATEKELEALLSLASRTDG
                     WDPPIVKTVAIENQGLEALAEAIDKFQQTQLSVAANSERRRAIARWRIVELLRERLLA
                     RVLASNSSVAMLEQLADEVATRRRDPYSAVEELLKI"
     gene            3169..>3534
                     /gene="mce"
                     /locus_tag="VFO72_09655"
     CDS             3169..>3534
                     /gene="mce"
                     /locus_tag="VFO72_09655"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET9479599.1"
                     /translation="MKVDHIGIATPTIDEGLAIWRDALGLTPDSTEEVIEQGVRVCML
                     PLGDTHVELLEPLSAESAVGKFLAKRGPGIHHIAIEVRDINASLAELRNKGARLIDET
                     PRAGARGCLVAFVHPSSTNG"
BASE COUNT          825 a          901 c         1100 g          708 t
ORIGIN      
        1 cggagttgcc gagcgttgag gattttcacg acttgagtgg cggcgggtct taataagttg
       61 tggagcaacg tctgcaaaaa ctcatcgcca cggcaggcat cgcctcgcgc aggcacgcgg
      121 aagaactcat tacagccggc cgtgtgacgg ttaatggcga gatcgtcaga gagcttggca
      181 cgaaggcaga tccagaacgc gatcacatca aagtaaacgg caagctcatc aatcctcagc
      241 tcaaagcacg cgacaaggtc tacgtcctgc tcaacaagcc gcgaggttat ctgtcgagcg
      301 tttctgatcc ggaaggacgc ccgctcgtta ccgaactgtt gccggcttca ctgggacgcc
      361 tgcatccggt cggtcgtctt gacttcaaca cagaaggttt actgctgctg accaacgacg
      421 gtgactttac caactacgtt accgcggctc ggaatcgcgt cgagaaggtg tacgaagtta
      481 aagtcaaagg cgtgccgtct gacggcgcga ttcaacgatt gcgaaaaggc gtgaggctcg
      541 aagacggcac ccgcacggcg ccggcgaaga tcacgaacct cggcgagacg cagaccaacg
      601 catggtacga agtgctgctt caccagggac gcaaccaaca agtgcgccgg atgtttgagt
      661 tgatcggcca ttctgtgttg aagttgagac gcgtgcggat tgggtttctg accgacgaca
      721 agttgaagcc gggtgagtgg cgttttctgt ctccctcgga agccgggcgt ttgatgaagc
      781 cgcaaagaca cagtcagtag gacaggcatt cttgcctgta actgcgattg caaaagaaag
      841 acagacaaga atgtctgtcc tactgagctg ccgattgcaa aagaaagaca gacaagaatg
      901 tctgtcctac tgagagcgag aacaaagagt accttatgca ttttgaatta actgaagaac
      961 aacaacaggt gaagtccagc gtgcgcgagt ttgcggagtc ggagatcgcg cctcacgtgc
     1021 tggagtggga cgagagccag cattttccga tcgagttaaa gccaaagttg gctgaactgg
     1081 gattgatggg cgtgatcttt ccggaagagt acggcggcgc cggcatgggc tatgtcgagt
     1141 acgcgaccat catcgaagaa ctctcccgtg tagatggatc ggtgggcatc tcgatcgcgg
     1201 cgcacaactc gctctgctcg aatcatattt ataaatatgg cacggaagag caacggcgaa
     1261 agtatctggt tccgctcgcc agcggtgaac atcttggtgc gtggggcctg actgaaccga
     1321 gcgccggttc agacgcgtcc ggaacaaaat cgactgccgt acgcgctgac ggtggttgga
     1381 ctgtgaacgg ttcgaaaaac ttcatcactc acgctattca cggtgacacg tgcgttgcca
     1441 tggccgtgac tgacagagcc aaacgcagca aaggcatctc ggcttttatc ttcgagaaag
     1501 gcatgaaggg tttttcgccg tcgaagaaag agaacaagct cggactgcgc gcctcggaaa
     1561 cggcgagcgt cgttttcgaa gactgttttg tgcctgacaa tcaactcctc ggggaagaag
     1621 gacagggatt tgtgaacgcg atggagatcc tcgacggcgg gcgcatctcg atcgcggcgt
     1681 tggcagtggg tattgcgcag ggcgcgtatg agtcggcgct gcgttatgcg caggagcggc
     1741 agcagttcgg caaaccgatc gcggagtttc aggcgatcca gttcaaactg gcgaacatgg
     1801 cgacgcagat cgacgccgcg cgattgttga tgtaccgcgc cgcgtggatg aaggatcagg
     1861 gacgccggac gacgaaagag tcgtcgatgg cgaagctgtt tgcttccgag atcagtgttg
     1921 aagtttgtga agaggcgatt cagattcacg gcggttacgg ctacacgaag gactacccgc
     1981 cggagaaata ctggcgcgac tcgaagctct gcacgatcgg cgagggaacg tcggagattc
     2041 aacgcatcat catcgctcgc gaactcttgc gacaagtttg agattgccga tttagtcatt
     2101 gtcgattgcc aattgccgat tgccgatttg ggccgctgct acatgagtct aaaaatcgac
     2161 aatcaacaat cggaaatcga caatgactaa atcggcaatc gaaaatcgac aatgactaca
     2221 aaaagcctaa gcgagcgagt caggcaggga gacgcagccg cagtggcgcg cgccatctcg
     2281 aagatcgagg acggttctga cggcgccgcc gcgttgatga aagagatcta ccagctctcg
     2341 cgcggcgcgt tggtcgtggg aatcaccggc gccccgggcg cgggaaagtc gtcgctggtc
     2401 gataaactcg cgctgcttta ccggcaacgc ggcgaacgcg tcggtatcgt cgcggtcgat
     2461 ccttccagcc ccttttcagg cggcgcaatc ctcggtgacc gcatccgaat gcaggcgctc
     2521 gggctggacg agggagtctt catccgctcg atggcgacgc gtggcaatct cggcggactc
     2581 gcgcgcgcga cagtcgacgc ggtggcgata ctggatgcag ccggttatgc aaaagtgatt
     2641 atagagaccg tgggcgtggg gcaggacgag gttgagatcg tcaaggctgc ggacgtttcg
     2701 atcgtcgttc tggtgccggg catgggcgac gacatccagg cgataaaggc cggcatcatg
     2761 gagatcggcg atatcttcgt gatcaataag gccgatcgcg acggggttta cgccacggaa
     2821 aaggagctgg aagcgctgtt atcgctggcc tcgcgcacgg acggctggga cccgccgatt
     2881 gtgaagacgg tggcgatcga aaaccagggc ctggaagcgc tagcggaggc aatcgataaa
     2941 ttccagcaaa cgcagttgag tgttgcggca aacagcgagc gccgtcgtgc tatcgcccgc
     3001 tggcgtattg tcgaattatt gcgggagcga cttttggcgc gggttttggc atcaaattcc
     3061 tccgttgcta tgctggaaca gcttgcggac gaagttgcaa cccggcggcg agacccgtat
     3121 tctgcggtgg aggaactgct gaaaatttag tagtgacaac ggacggaaat gaaggtcgac
     3181 catatcggca tcgccacgcc caccattgac gaaggtctcg cgatctggcg cgatgctttg
     3241 ggtttgacac ccgattcgac tgaagaggtg attgagcagg gtgtcagagt ttgtatgttg
     3301 ccgctcggcg acacccacgt cgaattgctc gaaccgctga gcgccgaatc tgcggtcgga
     3361 aaatttttgg ctaaacgtgg ccccggcatt catcacatcg caattgaagt tcgcgacatc
     3421 aatgcatcgc tcgcggaatt gagaaacaag ggcgcacggc tgatcgacga aactccacgc
     3481 gctggcgcgc gcggatgttt ggtcgctttt gttcacccgt cgtctacgaa tgga
//
LOCUS       DASTEY010000552         6823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_358363, whole genome shotgun sequence.
ACCESSION   DASTEY010000552 DASTEY010000000
VERSION     DASTEY010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358363"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFO72_09660"
     CDS             <1..847
                     /locus_tag="VFO72_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9479600.1"
                     /translation="LSCLRMIIHLLDGTYELFRHFYGIRRFKKTDPPFGAVSGVLTTV
                     AQMLDEGATHVGVATDHIIESFRNALWPGYKTGAGVDRALKAQFVPLEESLLAMGVTV
                     WPMVELEADDALASAAYLAARDKRVEKVCIWTPDKDLGQCVVDDRVVQIDRRSGTIRN
                     AEGVRAKFGVDPQFIPDYLALVGDSADGYPGISGCGPKTAAQLINRYGPLEDFPPNVL
                     SDANRTLALLFKELATLKTDAPLFDNVDELQWRGATPEFESVTERIGNPRLLTKVRRL
                     ERNLG"
     gene            828..2258
                     /locus_tag="VFO72_09665"
     CDS             828..2258
                     /locus_tag="VFO72_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9479601.1"
                     /translation="MKEISGKSRFDRSIIEGPLRPAVWKIAWPTMLTNVIGGLQGIVD
                     HALVGNLVGYKANAAIGVSWQIFIVVIVFISSLFTGMSVLVARFAGAGDEEKVNRTVY
                     QAFLTAIFIALGIMAPIGYLVSPSLLNLVNAAPEVKAEALPFLRIMFVFSVGMMIFFM
                     FSTALRSAGDARTPMMLGIMMTVLNLVLNILLIRGLGPIPAFGTMGSAMGTCLASGAI
                     AVYALGKMWSGDWVVSIPRGERGQSLGQRFGPDWGIIKAIFRFGLPTGIQGIAMNIGG
                     VFMLAFIGSLAQSAAAQAAFAVSYTELFSLVTWTSVGLMGAAAAVAGQNLGAGNPDRA
                     DAAVHVAARFGFAGAAFVGLVFLFFPRQLLAVFGMNEPDVVEIGVQLLRVLSLSGLFI
                     AVALTYTGGLQGTGDTRSPLYISIISQIVVPLGTCFVIQQTSTLDPIDIWIAILAGHA
                     TRCLLSVLRFKQGRWRTIAVNIESKS"
     gene            complement(2366..3193)
                     /locus_tag="VFO72_09670"
     CDS             complement(2366..3193)
                     /locus_tag="VFO72_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9479602.1"
                     /translation="MPSTRRKPQQVAEEENAGARPFWSGTLTFGLVSVPVNLFPAARA
                     NRASLRMLGPDGQPLARKYYAEKSGKDLEADEVVRGYEIKKDKYIVVTDEELERLEPE
                     KTRDINLTQFVPADSIPPLYFERGYFLTPAAGSQKAYKLLAETMQKNELAGIATFVMR
                     GKEYLVAIFSERGILRAETMRFPDEIRSPEDVGLPKKKAAPAATVRKFEKLIANKSKK
                     QFSPTKLADKQTDDLLKLVKKKEKQRGNVVRVEPEADKDHKVIDLVQVLKKSLGGKK"
     gene            complement(3186..3971)
                     /locus_tag="VFO72_09675"
     CDS             complement(3186..3971)
                     /locus_tag="VFO72_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9479603.1"
                     /translation="MAARAIWKGQLKVGTTKIPVKLYSAVQDKTVRFHILDDKHKQRV
                     KQHMIDPDSSEEVASEEIQKGYEVEPGKFVILTEEDLEQLAPPPSRDIEIVEFVPQAE
                     ISQQWYDRPYYLGPDNGDKDYFALVEALNNKKSEGIAHWVMRNKYYAGGLRPVDDYLF
                     LFTLRDAKEVILADQLPKPSGAEPTQKELTMAKQLVEMLKGEFNPKDYKDEYRKRVME
                     FIERKAKGHAPRLRAVKTKRKSGDLDNVLAKSIAALKKEKRAA"
     gene            complement(4215..4448)
                     /locus_tag="VFO72_09680"
     CDS             complement(4215..4448)
                     /locus_tag="VFO72_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479604.1"
                     /translation="MTLDQQVDDKGTDQQEAKRMLTALRDRGFEGDNEKLAVALGRSS
                     EQVQGWIDGTETIDDDVVMKARGIALNRGVEVG"
     gene            4716..5129
                     /locus_tag="VFO72_09685"
     CDS             4716..5129
                     /locus_tag="VFO72_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479605.1"
                     /translation="MDDSNQTAIGQCPGARLWLTQGLRSGDVIGSFTTQVFKLDGEDV
                     ILSTATDKVEQLEAEQRCHHASRLATLVEQMAAGLAHEVKNQLAGIKGVALSTVQRTA
                     VAFQACVSFETHVGQGSSFELAFPLPATHPESHER"
     gene            5119..6345
                     /locus_tag="VFO72_09690"
     CDS             5119..6345
                     /locus_tag="VFO72_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9479606.1"
                     /translation="MSANPQTVVVVDDDPTIRSTLAEALRDWGYEPREAATLAETLAL
                     VSRERPAAVLLDVKMPDGSGISILDELKTSSPETVVIIITGYLNSRSAFEAGLRQAYG
                     FLTKPLDETEVRSMLEEALAGVRVAKSRKEDAPHGRSSPPFDSSETKRGRPQHETTAP
                     LGNLILKAMNLLGLNYKDVVVESERLARLNNNSDMRIGKSTLGDIISGSIRQPSAAKL
                     DVLRIILNLSHAETDAALGLQPERRFAEQLELTRTRTHEVNAEIVTRHRKIKVPILRE
                     DTDLNESQLLEAAVKRWATIEVEYLGSFYPPHFCYLVVGEDDHNATPIAPPGSRLLVN
                     KLLNRIRPAENLSYHERELYYLMTPRGFTCAYLETAPGDKVVLVPHPLSRNVREEFKR
                     ADVKVIGQVVGVLYPR"
     gene            6638..>6823
                     /locus_tag="VFO72_09695"
     CDS             6638..>6823
                     /locus_tag="VFO72_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479607.1"
                     /translation="MAAGQQLKLLRNRRNITVREVEQASRRIADAKKDKRFQISNGWL
                     AQLENGVSEPSICKLFSL"
BASE COUNT         1401 a         1905 c         1866 g         1651 t
ORIGIN      
        1 tttaagctgc ctgcgaatga ttattcatct gctcgatggt acctacgagc tgtttcgaca
       61 cttttacgga atccgtcgtt tcaagaagac tgatccaccg ttcggcgccg tctccggcgt
      121 cttgaccacc gtggcgcaga tgcttgacga aggcgcaacg cacgtcggcg tagcgactga
      181 ccacatcatc gagtcgtttc gtaacgcgtt gtggcccggt tacaagaccg gcgccggcgt
      241 cgatcgggcg ttgaaggcgc agttcgttcc gctcgaggaa tccttgttag caatgggcgt
      301 gacggtttgg ccaatggtcg aactcgaggc cgacgacgcg ctggcgtctg ctgcatatct
      361 cgcggcgcgc gacaaacgag tagagaaagt ctgtatctgg actcccgata aggacctggg
      421 ccaatgcgtc gtagacgatc gtgtcgttca gatcgatcgg cgcagtggta ccattcgaaa
      481 cgccgaggga gtacgcgcga aatttggcgt cgacccgcag ttcattcccg attatctcgc
      541 gctggtgggc gactctgctg acggatatcc aggcatttcc ggttgtggtc caaagacagc
      601 tgcgcagttg atcaaccggt atggaccact ggaggacttc ccgcctaatg ttctcagcga
      661 tgcgaatcgt acactggcgt tactcttcaa ggagctggcg acgttgaaaa ccgacgcgcc
      721 gcttttcgat aacgtcgatg agttgcaatg gcgcggcgcg actcccgagt ttgaatctgt
      781 gacggaaaga atcggcaatc cgcggttgtt gacaaaggta cgcaggcttg aaagaaatct
      841 cgggtaagtc gcgcttcgat cgatcgatca tcgagggacc gctgcgcccg gcggtctgga
      901 agatcgcgtg gccgacgatg ctgaccaacg tgatcggcgg tttgcagggc atagtggacc
      961 atgcgctcgt cggcaatcta gtcggctaca aggcgaacgc ggcgattggc gttagctggc
     1021 aaatctttat cgtcgtgatc gtgtttatct cgtcgctgtt taccggcatg agcgtgctgg
     1081 tggcgcgttt cgcgggcgct ggcgatgaag agaaggtgaa ccgcacggta tatcaggcat
     1141 ttctcaccgc gatcttcatt gcgctcggga tcatggcgcc gattggatat ctcgtgtcgc
     1201 cgagtttgct caacctcgtc aatgcggcgc cggaggtgaa agcagaagcg ttgccgttcc
     1261 tgcgcatcat gttcgtgttt agtgtgggca tgatgatctt cttcatgttc agcaccgcgc
     1321 ttcgctccgc cggcgacgcg cgtacaccaa tgatgctggg aatcatgatg actgtgctga
     1381 acctcgtcct gaacatattg ctcattcgag ggctgggtcc gattccagcg tttggaacca
     1441 tgggttcagc gatgggcacg tgcctcgctt ccggtgcaat cgcagtttac gcactcggca
     1501 aaatgtggag cggcgactgg gttgtatcca ttccgcgtgg cgagcggggg caaagtttgg
     1561 gccagcggtt tgggccggac tggggcatca tcaaagcgat ctttcgtttt ggtctaccga
     1621 cagggattca gggaatcgcg atgaacatcg gtggcgtgtt catgctcgcg ttcatcggtt
     1681 cacttgcgca aagcgcggcg gcgcaggcgg cgtttgctgt gtcgtacacc gaactttttt
     1741 ccctcgtgac ctggacgtcc gtcggattaa tgggcgcggc cgccgctgtt gcgggacaga
     1801 acctcggcgc cggcaatccc gatcgggctg atgcggctgt gcacgtcgcg gcacgttttg
     1861 gttttgcggg tgctgcattt gtcggactgg tgtttctgtt cttcccgcgg caactgctgg
     1921 cggtgtttgg catgaacgaa cctgacgttg tcgagatcgg cgtgcaattg ctgcgtgtgc
     1981 tcagtctttc cgggctgttt atcgcggtcg ccttgacgta taccggcggt ttgcagggaa
     2041 ccggcgatac gcgcagcccg ctgtacatct cgatcatctc gcagatcgtc gtcccgctcg
     2101 gcacatgttt cgtaattcag cagacgagca cacttgatcc gatcgatatc tggatcgcga
     2161 tcctcgcggg tcacgcgacg cgctgcttgt tgagcgtgct tcgcttcaaa cagggcaggt
     2221 ggcgcactat cgctgtaaac attgagtcta aaagttagtg aaccggctct ttcctgggac
     2281 cgcaggcgtc tcgcttgcaa agcagcgata agcgcccggt cggttccgag caggcgagac
     2341 gcctgtggtc ccaggaaaga gccggttact tcttgccgcc gaggctcttc ttgagcacct
     2401 gaaccaggtc gatgactttg tgatccttat cggcttcggg ttccacgcgc acaacattgc
     2461 cgcgttgctt ctctttcttc ttcaccagct tcaacagatc gtcagtctgt ttgtccgcca
     2521 gcttcgttgg tgagaactgt ttcttcgact tattcgcgat aagcttctca aacttgcgta
     2581 cggtcgcagc cggcgccgcc ttctttttcg gcaaccccac gtcttccggc gagcgaatct
     2641 cgtcggggaa acgcatcgtt tcggctcgca gaattcccct ttccgagaag attgctacga
     2701 gatattcctt cccgcgcatc acgaacgtcg cgatgcccgc gagctcgttc ttctgcatcg
     2761 tctcggctaa caacttgtat gctttctgcg aacccgcggc aggcgtcagg aaataaccgc
     2821 gttcgaaata gaggggcgga atagaatcgg cggggacgaa ctgcgtcaga ttgatgtcgc
     2881 gtgtcttctc cggttccaag cgctccaact cttcatctgt gacgacaatg tacttgtctt
     2941 tcttgatctc atagccgcgc acgacttcgt ctgcctccag atcttttccg ctcttctcag
     3001 cgtaatactt tcgcgccaac ggctggccgt caggtccaag catccgcaac gacgcgcgat
     3061 tcgcacgtgc cgccggaaac agattgaccg gcacactcac caggccgaac gtcaacgttc
     3121 cggaccaaaa cggtctggct ccggcatttt cttcttcagc aacctgctgc ggtttgcgtc
     3181 tcgtgctagg cagcgcgttt ctccttcttc aatgcagcaa tactcttcgc gagtacgtta
     3241 tcgagatcgc cactcttccg tttagttttg accgcacgca gccgcggcgc gtggcctttg
     3301 gctttacgtt cgatgaactc catcacgcgt ttgcgatact cgtctttgta atccttcgga
     3361 ttgaactctc ccttcagcat ctcgacaagc tgcttcgcca tggtcaactc tttctgcgta
     3421 ggctccgcac ctgaaggttt cggcagttga tccgcgagaa ttacttcctt cgcgtcgcgc
     3481 aaagtgaaga gaaacaggta gtcgtctacc gggcggagtc cgccggcgta gtacttgttc
     3541 cgcatcaccc aatgcgcaat gccttcactc ttcttgttgt tgagagcttc gacgagcgcg
     3601 aagtagtctt tatcaccatt gtcaggaccg aggtaataag gccggtcata ccactgctgg
     3661 ctgatttctg cttgcggtac aaactcaacg atctcaatgt cgcgcgaggg cggcggtgct
     3721 agttgctcga gatcttcctc ggtgagtatc acgaattttc cgggttcaac ttcgtagcct
     3781 ttctgtatct cctcgctcgc gacttcctcg cttgaatcgg gatcgatcat gtgctgcttc
     3841 acgcgttgtt tatgtttgtc gtcgagaatg tgaaagcgaa cggtcttgtc ctgcaccgcc
     3901 gaatacagtt tgacgggtat cttcgtcgtg ccaactttca attggccctt ccagattgct
     3961 cgggctgcca tttaacgtga ctccttttcc gcagaacgag atgattcaca gccgtgacag
     4021 agcagttcga cggagacgct ttgctgtatg cataatgaac agacattcgc gtcgcaatcg
     4081 agacagtaag agcctgtttg gtatgcgtac gactggtgac acgacgaaca gatctcagtg
     4141 tcaccatcga accaccacgg tgcagtgctg cttgtttgtt gtttggcgcg cctcagctct
     4201 gatcctccga atctttaacc cacttcgacc ccgcgattga gggcgattcc tctggccttc
     4261 atcaccacgt cgtcgtcaat agtttcagtg ccgtcgatcc atccctggac ctgttcgctg
     4321 gacctcccca acgccactgc gagcttttcg ttatcacctt caaagccgcg atcgcgcagc
     4381 gccgtcagca ttcgtttggc ctcttgctga tcggtcccct tatcatcaac ttgctggtcg
     4441 agcgtcataa cagactcctg ccttcgattc tgttctgttc cagatcactt attcaatgct
     4501 atgagaacct agcgcaaacg aaatgccaga gcacggacgt gcgcttcatg aacttcgata
     4561 ttctcaaaag cagaatcatg gtgtaagatt agcgcgcttt aacctccctg aacgatccca
     4621 gagcaacata acgagtgcgg tccccactag tagcttgagt aatcgcccac ttaagtagtg
     4681 cgtccgctga acctgcagaa ggtatggacc aagctgtgga cgatagcaac caaactgcta
     4741 ttggtcagtg tccgggtgct cgcctttggt tgactcaggg cttgcgctct ggagacgtca
     4801 tcggatcgtt cacgactcag gtgttcaaac tcgatggcga agacgtcatc ctctcaaccg
     4861 ccactgacaa agtagaacaa ttggaggcag agcaacgatg ccatcacgcc agcaggctcg
     4921 cgacactcgt cgaacaaatg gccgctggtc tcgctcacga agtaaaaaat cagctcgccg
     4981 ggataaaagg tgtcgcactt tcaactgtgc aacggacggc cgtggcgttt caggcctgcg
     5041 tcagttttga aactcacgta gggcagggat cctcattcga acttgcattc cctttgccgg
     5101 ctactcaccc ggaatcgcat gagcgctaat ccgcaaacag tcgtcgtggt agatgacgat
     5161 cccactattc gctcgactct cgccgaagca ttgcgtgatt ggggttacga accgcgtgag
     5221 gctgctacgc tcgccgagac gttggcgctc gtgagtcgcg aacgacctgc cgctgtgttg
     5281 ctcgacgtga agatgcccga tggatccggg atcagtattc ttgacgaact gaaaacgagt
     5341 tcgcctgaaa ccgtggtcat cattattacc gggtacctga attcgcgcag tgctttcgag
     5401 gcagggttgc gacaggctta cggttttctg acgaagcctc tcgacgaaac agaagttcgc
     5461 tcgatgctgg aggaagcgct cgccggtgtc cgagtcgcga agtcacggaa agaagatgct
     5521 ccacacgggc gtagctcacc tccgtttgat tcgagcgaga ctaaacgtgg ccggccgcaa
     5581 catgaaacta cagcaccgct tggcaacctg attctcaaag caatgaactt gcttggactc
     5641 aactacaaag acgtcgtagt cgaaagcgaa cgtctggcca ggctcaataa caactcagac
     5701 atgcgcatcg gcaaatcgac tttgggcgac atcatcagcg gcagcattcg tcagccaagt
     5761 gcggcaaagc tcgacgtgct gcggatcatc ctcaatcttt cgcacgccga gacggatgcc
     5821 gcgctcggtc tacagcccga acgacgcttt gcggaacaac tcgagctcac gcgcacacgc
     5881 acacatgaag ttaacgctga gatcgttacg cgtcatcgta aaatcaaagt accaattctc
     5941 cgagaagata ctgatctcaa cgagtctcaa cttcttgaag cggcggtgaa acgttgggcc
     6001 actattgaag tcgaatatct tggttcgttc tacccgccac atttttgcta cctggttgtc
     6061 ggtgaagacg accacaacgc cacgccaatc gctccacccg gcagtcgttt gttggtcaat
     6121 aaacttctga acagaattcg tccggctgaa aacctcagtt atcatgagcg cgaactctat
     6181 tacctgatga cgccacgcgg cttcacctgt gcttatctgg aaaccgcgcc cggcgataag
     6241 gttgtactgg ttcctcatcc gctatcccgg aatgtgcgcg aagagtttaa aagagcggac
     6301 gtaaaggtga ttggccaggt cgttggagtg ctgtatccgc gatgaattgt ccgtgatccg
     6361 gaacaacgat tcgacggcca aactctgact aatcgtgggc catgacacag gttgggagtg
     6421 atctcgctgc gaagtgttgt tcaagtcctc gcattgccgg cgtggcgttg ccggcgcaac
     6481 ttcgcccgtt tgtaaaactt aggtctccgg cataccggac aaagaaagtt tttgctcgca
     6541 gtcggatctg ctattgggct aaatttcgcg tctcgcccaa attcgcatac acctcctata
     6601 caacttctga gctcagccca ggtgctgtga ggacactgtg gcggcgggtc agcaactcaa
     6661 gctcctgcgt aacaggcgca acatcacggt cagggaagtc gaacaggcaa gccggcgaat
     6721 tgccgacgcc aaaaaagaca agcgtttcca gatctcaaac ggctggttgg cccaactgga
     6781 aaatggcgtt tccgagccga gtatttgcaa gctgttcagt ctg
//
LOCUS       DASTEY010000553         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1528781, whole genome shotgun sequence.
ACCESSION   DASTEY010000553 DASTEY010000000
VERSION     DASTEY010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528781"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFO72_09700"
     CDS             complement(<1..588)
                     /locus_tag="VFO72_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9479608.1"
                     /translation="MAIAKEPEFTEAIETVDNDQQLQIPRELPVLPLRDIVIYPFMIV
                     PLFVSREKSIRAVDEALAENRMILLASQKDLDKEEPTAEDLYAVGTAAVIMRMLKLPD
                     GRIRILVQGLARAQIESVDATGEYLRSKVSVMQEVLPPERSLEVEALVRNVRASMEKA
                     INLGKNISPEVMAIIANLDDAGRLADLSASNLELKV"
     gene            complement(575..1054)
                     /locus_tag="VFO72_09705"
     CDS             complement(575..1054)
                     /locus_tag="VFO72_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9479609.1"
                     /translation="MIKPIRATGLERLELQRLRDRVGRLYAALQEATEAENPLASGTW
                     APPVDLCETEKLISIRVELPGVTAEQIKIGLCNTKLRISGEKKRRPNRRKILSYLCSE
                     RSFGKFERIVPLRWTFSIREASAELSNGVLHICLPKMEDRRGEEVLIAVKDTGHGDS"
     gene            complement(1079..1558)
                     /gene="nrdR"
                     /locus_tag="VFO72_09710"
     CDS             complement(1079..1558)
                     /gene="nrdR"
                     /locus_tag="VFO72_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET9479610.1"
                     /translation="MKCPFCAYLEDKVVDSRESREGDAIRRRRECLRCERRFTSYERI
                     DEIPYMVVKKDGRRETFDRNKVMAGLLRACEKRPVPSTKLDSIVNAIEKYVQESPERE
                     RPTSKIGEMIMRRLKELDKVAYVRFASVYLEFEDVSEFMNELKNLVRTRNSGPSGKK"
     gene            complement(1560..>2500)
                     /locus_tag="VFO72_09715"
     CDS             complement(1560..>2500)
                     /locus_tag="VFO72_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9479611.1"
                     /translation="YLLTAAVLIICAAYAFRAFRWAALLKPLSAARFADLFAATTIGF
                     SGVFLVGRAGEFVRPVVLSMRDPRVRPSASLVTIMVERIYDLTAVALMFAVNLIWFAP
                     PPSAEVSFDQVRTAGVGLVGAIILGIAFLTWFRKRSSVVINFFERLFSRWRFVPRRLS
                     QLVLRLLEQLAQALRVLVNFRELAETTGWTVLVWVGIATANLLVMRAFHLQIGVAEAI
                     FVLGWSLVGSLVPTPGGAAGAFHAATAGALIFLGVKKETAAALSIVLHLVVFGPAVLF
                     GLFYVIRGDLSLAKLRAMISPQPTEDLIDEVPERVH"
BASE COUNT          577 a          736 c          655 g          532 t
ORIGIN      
        1 aactttcagc tcgagattgg aagcggacaa atccgcgaga cgtcccgcgt cgtcgaggtt
       61 ggcaatgatg gccatcactt caggcgaaat gttcttgccg agattgatgg ccttctccat
      121 cgaggcgcgt acgttgcgca cgagagcttc aacttccagc gaacgttccg gaggcaacac
      181 ttcctgcatc acgctcactt tcgagcgcag gtactcgcca gtggcgtcga cagattcgat
      241 ctgcgcacgc gccaggccct ggaccaggat gcggatacgc ccgtcaggca acttgagcat
      301 ccgcatgatg actgccgcag taccgacggc gtagagatcc tcggctgtcg gttcttcctt
      361 atcgagatcc ttctgtgacg cgagcaggat catccggttc tcagctaatg cttcgtcaac
      421 tgcccggatc gacttctcgc gtgacacaaa caaaggaacg atcatgaacg ggtagatcac
      481 gatgtcgcgc aagggcagca ccggcagctc gcgaggaatc tgcaactgtt gatcgttgtc
      541 gactgtttcg attgcttccg taaactctgg ttctttagct atcgccatgg cccgtgtcct
      601 ttactgctat caggacctcc tctccccttc tgtcctccat ctttggaagg caaatgtgaa
      661 gcacaccgtt gctaagctcc gccgacgcct cgcggatgct gaaggtccaa cgcaacggca
      721 cgatgcgttc gaacttgcca aagctgcgct ccgaacacaa gtaagaaaga atcttccggc
      781 ggttgggccg tcttttcttt tcacccgaga ttctgagttt agtgttgcaa agaccgattt
      841 ttatctgttc ggcagtcaca cccggtagct ccactcgaat cgaaatcaat ttctccgttt
      901 cgcaaagatc taccggcggc gcccaggttc cggaggctaa cgggttctcg gcttcggtcg
      961 cttcctgcaa agccgcatag agccgcccca cgcggtcgcg cagtcgctgc aactcgagcc
     1021 gttctaatcc ggtcgcacga atgggtttga tcattgccag ttccccttgc tcgtaatact
     1081 acttcttgcc tgatggtccg ctgttcctcg tccgcaccag gttcttgagc tcattcatga
     1141 attcagagac gtcttcaaac tccaaataca ccgaagcgaa ccgaacatat gcgactttgt
     1201 ccagttcttt caagcgacgc atgatcatct ctccaatctt gctggtggga cgttcgcgtt
     1261 cgggcgattc ctgaacatac ttctcgatcg cgttgacgat gctatcaagt ttagttgaag
     1321 gcacgggacg cttctcgcag gcgcgcaaca ggccggccat taccttgttg cgatcgaagg
     1381 tctctcgacg gccgtccttt ttaacgacca tgtaaggtat ctcatcgatg cgctcgtagg
     1441 acgtgaatct tcgctcacac ctgagacatt ctcggcggcg ccggatggcg tcgccttcgc
     1501 gcgactcgcg cgagtcaacc accttgtctt caagatacgc acagaatgga catttcattt
     1561 tagtgaaccc tttccgggac ttcgtcaatg agatcctcag ttggctgagg tgaaatcatg
     1621 gcgcgcaatt tcgccagact caggtcacca cgaattacat agaaaagacc aaacaaaact
     1681 gctggaccaa aaaccacaag gtgcaaaaca atcgagagtg ccgcggcggt ctctttcttc
     1741 acgccgagaa atatcaaagc acccgcagtc gctgcgtgaa aagcgccggc cgcgccgccg
     1801 ggtgtcggca cgagtgaacc caccagcgac cagcccaaca cgaatatcgc ttccgcaact
     1861 ccaatctgaa gatgaaaagc tcgcatcacg agcagattcg ccgtggctat gccaacccag
     1921 accaaaacag tccaaccggt cgtttctgcc agttcacgaa aattcacgag tacgcgaagt
     1981 gcctgggcga gttgttcgag gagcctcagg acaagctgcg acagccgtcg tggcacaaag
     2041 cgccagcgcg agaagagtcg ttcgaaaaag tttataacga ctgacgagcg tttcctgaac
     2101 cacgtaagaa aggcgatgcc gagaatgatg gcgccgacca gtccgactcc ggcggttcgc
     2161 acctggtcga atgaaacttc cgctgagggc ggtggcgcga accagatcaa gttgaccgca
     2221 aacatcagcg ccacggcggt caggtcataa atacgttcga ccatgatcgt gaccagcgaa
     2281 gcactcggtc tcacacgcgg atcgcgcatt gagagcacca ccggtctcac gaactcgccc
     2341 gcccggccga ccagaaagac gccgctaaag ccgatggttg tggcggcaaa gaggtcggca
     2401 aaccgggctg cgctcagagg tttcagtagc gccgcccagc gaaatgcccg aaaagcataa
     2461 gccgcacaga tgattagtac ggcggcggtg agcagatatg
//
LOCUS       DASTEY010000554         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1264851, whole genome shotgun sequence.
ACCESSION   DASTEY010000554 DASTEY010000000
VERSION     DASTEY010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264851"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFO72_09720"
     CDS             <1..822
                     /locus_tag="VFO72_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET9479612.1"
                     /translation="LRIMQHAIDISIEAHQRAWAIAGDAKWEYEVDAIVAYTFKLRNA
                     DNWGYPDIVGCGPNATTLHYIESQGPVKPGDLLLMDVGAEYDHYTADVTRTFPINGKF
                     TPIQAEVYQIVYDAQEAAARAAKPGATLSDVNRASTEMIKDGLLKLGLITDRNSMQHR
                     LWFMHNTSHWLGMNVHDVGGGSKIVPGVVFTNEPGIYVRLDALDQMPDRWKQEDWERF
                     KTAVRPAFEKYKGIGVRIEDDLLITADGVKWLTAALPRRINEIEEFIAKARRQSE"
     gene            complement(854..1378)
                     /locus_tag="VFO72_09725"
     CDS             complement(854..1378)
                     /locus_tag="VFO72_09725"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET9479613.1"
                     /translation="MKLEGTHELEATRERVYQSLVDPEVLQRCIPGCERLERTGENTF
                     STTIRAGVGSIKGVFEGSVRLEDLREPEHFRMVVDGRGAPGFLKGSGDLDLAQEGGFT
                     KVNYAGDVQVGGTIASVGQRMIQGTAKMMAAQFFTCLGAEAKVAPGEPPPKHGFFRNA
                     LRWFSGWLRKLFRK"
     gene            complement(1375..2241)
                     /locus_tag="VFO72_09730"
     CDS             complement(1375..2241)
                     /locus_tag="VFO72_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9479614.1"
                     /translation="MIPAPFDYQAPATLEEAVSLLSVDPDGAKVLAGGHSLIPAMKLR
                     LAQPQLLIDIARIKSLAYIREEGNQILIGATTTHYQLESSDLLKQICPLLPACAKSIG
                     DVQVRNKGTIGGSIAHSDPAGDWPAAALALDADLVLVGPGGERIVKAANFFVDLLTTD
                     LQPGEILREIRIQKPSGRFGQAYQKVPHPASGFAVVGIAVHLAANDDGSCKAAQIGVT
                     GVGVKAYRAQAVEAALAGQSLDDQIVATASAHVCNGVDPNSDLYASGEYRCHLAQVHT
                     RRAIQAAKANLK"
     gene            complement(2273..4675)
                     /locus_tag="VFO72_09735"
     CDS             complement(2273..4675)
                     /locus_tag="VFO72_09735"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9479615.1"
                     /translation="MPNSYVGQRVRRTEDPRLIKGLAHYVDDISLPGTLHVAFVRSVY
                     AHARINSIDTSAALAVPGVVAVYTGKDVAEKIGPVPCASALPELKVPDHRVLGTDKVY
                     FVGHPIAAIVATDRYAARDAVDLVAVDYQDLPVVTDVEEAAAGGTVIHESFGDNIAYK
                     MTAGEGDIDAAFASADRTLKQRVLHKRLAPIAMEPRGVLARYFPGEEELTLWSSTQIP
                     HLMRTQVALMIGMPENKLRVITPEVGGGFGSKLNVYAEEALLGWISMQLNKPVKWIET
                     RRENILATIHGRGQAGDIEVAFKNDGRITGLRYNVFADLGAYHQLLTPAIPTLTGLML
                     SGAYKIPAIQINITGCFTNKMATDAYRGAGRPEATYVVERALDLVAAELNADPAEVRR
                     KNFPGKDEFPFHTATGLDYDSGDYEAALDKAQQIIGYAQLREEQKKARAEGRLIGVGV
                     STYVEICALGPSQAMPAGGWESATVRIEPTGKVTVLTGASPHGQGQETSFAQIVADEL
                     GVSINDVTVIHGDTAVVQYGIGTFGSRATAVGGTAVYVAVQKLKEKAEKIAAHILQAD
                     TTKLSFEGGKYSVKQAAAAGVTGSSDPVYPVGQGPAGALPEPQTEGRTSLTIAEIALA
                     AHLAKELPPDTEPGLSATYFFEPKNFTFPFGTHIAVVEVDRETGDIKFLRYVAVDDCG
                     KVINPMLVDGQVHGGIVQSIGQAMYEEVVYDEQGQLITGTLMDYALPRASHIPNFELD
                     RTETPTPVNPLGVKGVGEAGTIGATPAIVGAIVDALAPFGVRHLDMPIKPEDVWRVIS
                     RG"
     gene            complement(4768..5220)
                     /locus_tag="VFO72_09740"
     CDS             complement(4768..5220)
                     /locus_tag="VFO72_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9479616.1"
                     /translation="MSVNGVSRTDEVEPRMLLVHYLRDVLGLTGTHVGCETSLCGACT
                     IMLDGQTVKSCTLFAVQAEGASIKTIEGLATNGELHPVQEGFWERHGLQCGYCTPGMI
                     MSAAQLLDRNANPSREEICHGLDGNLCRCTGYQHIIEAVEYAAKKASA"
     gene            complement(5306..5626)
                     /locus_tag="VFO72_09745"
     CDS             complement(5306..5626)
                     /locus_tag="VFO72_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479617.1"
                     /translation="MAYTYEQLKHMTLAELREIAKGSEHEAVQGYTQMNKEHLIGALA
                     KDLGIQHEHHDVVGIDKSAIKSRIRDLKTQRDAALAAHDHAQLKVVRRTIHRLKRQIN
                     KATV"
     gene            5807..>6136
                     /locus_tag="VFO72_09750"
     CDS             5807..>6136
                     /locus_tag="VFO72_09750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9479618.1"
                     /translation="MKRLLKLSTVFIIALLLLTLTGLGGAIVDKLTFFGRVVRLYTQE
                     PDRTIAMPLKDVSKRYIADTWHAPRGTGRLHEGQDIFAPKGTPVLSATSGYVYNIGEN
                     NLGGQTVS"
BASE COUNT         1226 a         1761 c         1777 g         1372 t
ORIGIN      
        1 ctgcgcatca tgcaacacgc gatcgacatc agcatcgaag cgcaccagcg cgcctgggcg
       61 atcgccggcg acgcaaagtg ggaatacgaa gtcgacgcga tcgtggccta cacgttcaaa
      121 ctacggaacg ccgacaactg gggctatccc gacattgtcg gttgtggtcc aaacgcgacg
      181 acgcttcact acatcgagtc gcaaggtccc gtaaaacctg gtgacctgct attgatggac
      241 gttggtgctg aatacgacca ttacacggct gacgtcacgc gcacgtttcc gataaacggg
      301 aaattcacgc cgatacaggc tgaggtctat caaattgtct acgacgcaca ggaggcggca
      361 gcaagagcgg cgaaaccggg cgcgacgttg tcagatgtga accgtgcgag tacggaaatg
      421 atcaaagacg ggttactaaa acttggtctg atcaccgatc gcaattcaat gcagcaccgg
      481 ctgtggttta tgcacaacac gtcgcactgg ctcggaatga acgttcacga tgttggcgga
      541 ggatcgaaga tcgtgcctgg tgtagttttc actaatgaac cgggaatcta cgtacggctc
      601 gatgcactgg accagatgcc ggatcggtgg aaacaggaag actgggagag attcaagacg
      661 gctgttcggc cggcgtttga aaaatacaag gggattggcg tccggatcga agacgacttg
      721 ttgataacgg ctgacggtgt gaagtggctg acggccgcgt tgccgcgcag gatcaacgag
      781 atcgaggagt tcatcgcaaa ggcgcggcgg cagtcggagt gatttttgcc acggattaca
      841 cggattacac ggattacttt ctaaacaatt ttcgtagcca ccctgagaac cacctcaggg
      901 cgtttcgaaa aaatccgtgc tttggaggtg gttcgccggg cgctaccttc gcttccgcgc
      961 cgaggcaagt gaagaactgt gcggccatca tcttcgccgt gccctggatc atgcgctggc
     1021 ccacgctcgc gatcgttccg ccaacctgaa cgtcgccggc atagttcacc ttggtgaaac
     1081 cgccctcctg ggcgaggtcg agatcacccg aacccttcag aaatccaggc gcgcccctgc
     1141 catcaacaac catccgaaag tgttccggtt cgcgcaggtc ttcgagccgg acgcttccct
     1201 caaacacgcc cttgatcgaa ccgactcccg ccctgatggt ggtggaaaac gtgttctcac
     1261 ccgtgcgttc gagacgttcg caaccgggaa tacaacgctg caacacctcc ggatcgacca
     1321 gcgactgata gacgcgttcg cgcgtggctt cgagttcgtg agtgccttcg agtttcactt
     1381 gaggttggct ttggccgctt gaatcgcacg ccgcgtatgc acctgggcca ggtgacagcg
     1441 atactcgccg gacgcataca gatctgagtt tggatcgact ccgttgcaca catgcgccga
     1501 cgccgtcgcc actatctgat cgtcaagact ctggccggcg agtgccgctt cgactgcttg
     1561 cgcgcgatac gctttgacgc cgacacccgt tacaccgatt tgcgctgcct tgcacgatcc
     1621 gtcgtcgttc gcggcgagat gaacggcaat gccgacgacg gcaaaacccg aagcgggatg
     1681 cggcaccttt tgataagcct gtccgaaacg gccggagggt ttctgaattc ggatctcgcg
     1741 caggatctca ccaggctgca aatccgtcgt caaaagatcg acgaagaagt ttgcggcttt
     1801 aactatccgt tcgccgcctg gaccaaccag taccaggtcc gcatcgagcg ccagcgccgc
     1861 agcgggccag tcccctgcag gatccgagtg agcgatgctg ccgccgatcg ttcctttgtt
     1921 gcgcacctgc acgtcgccaa tgctcttcgc gcacgccggc aacaacgggc agatctgttt
     1981 gagcaggtct gacgattcga gttgatagtg cgtcgtggtc gcgccgatga gaatctggtt
     2041 gccttcttcg cgaatgtagg ccagcgactt gatgcgggcg atgtcgatca gcaactgtgg
     2101 ttgcgccaga cgcagtttca tcgccggaat caagctgtga ccaccggcta aaacctttgc
     2161 gccgtccgga tctaccgata gcaacgacac cgcctcttcc aaagtcgcgg gcgcctggta
     2221 atcaaatggt gcggggatca ttccttcact ccttcttcgt gttcgtctgt gttcagccgc
     2281 ggctgataac tctccaaaca tcttcgggct taatcggcat gtcgagatgg cgaacgccga
     2341 aaggcgccag cgcatcgacg atcgcgccga ctatcgcggg tgtcgcgccg attgttcccg
     2401 cctcacctac tcccttcaca ccgagcggat tcaccggcgt cggagtttcg gttcgatcga
     2461 gctcgaagtt cggaatatgt gaagcgcgcg gcaaggcata gtccattaac gtaccggtga
     2521 tcaactgccc ctgttcgtcg tagaccacct cttcgtacat cgcttggccg atcgattgaa
     2581 cgatgccgcc gtgtacctgc ccgtcgacca gcatcggatt gatcactttg ccgcagtcgt
     2641 cgacggccac ataacgcaga aacttaatgt cgccggtttc gcgatcgact tcgacgacgg
     2701 cgatgtgcgt gccgaacggg aacgtgaagt tctttggttc aaaaaagtaa gtggcagaga
     2761 gtcctggctc agtgtcgggc ggcaattctt tggctaggtg ggccgcgagc gcaatctcag
     2821 cgattgtgag actcgttcgt ccttcggtct gcggttccgg cagcgcgccc gcgggtccct
     2881 ggccgaccgg gtaaaccggg tcggaacttc ccgttacgcc tgcggcggct gcctgcttca
     2941 cggaatactt gccgccttcg aaagagagtt ttgtagtatc ggcttgcagt atgtgcgcgg
     3001 cgatcttctc ggctttctcc ttcagttttt gcaccgccac gtacacggcg gtgccgccca
     3061 cggccgtcgc gcggctgcca aacgtgccga tgccgtattg gaccacggcg gtgtcgccgt
     3121 gaatcacagt cacatcattg atgctgacgc ccaattcatc cgcgactatc tgtgcaaatg
     3181 aagtttcctg tccctgaccg tgtggcgagg cgcccgtcaa cacagtgact ttgcctgtcg
     3241 gttcgattcg cacggtggcg ctttcccaac cgccggccgg catggcctgg gatggaccaa
     3301 gcgcacagat ctcaacgtag gtcgaaactc cgacgccaat cagccggcct tccgcgcgtg
     3361 ctttcttctg ctcttctcgg agctgtgcgt aaccaatgat ctgctgcgcc ttgtcgagtg
     3421 cggcttcgta atcgccactg tcgtaatcga gaccggtggc cgtgtgaaac gggaattcgt
     3481 ccttaccggg gaagttcttg cggcgcactt cagccggatc cgcgttaagt tctgcggcga
     3541 ctaagtccaa agctcgctca acgacgtagg ttgcttccgg gcgaccggcg ccgcgatagg
     3601 cgtccgtggc catcttgttt gtgaagcaac cggtgatatt gatctgaatc gccggaatct
     3661 tgtaagcacc ggaaagcatc aagcccgtga gcgtcgggat cgcgggagtc agcagttgat
     3721 gatacgcgcc gagatccgcg aagacgttgt agcgcagccc cgtaatcctg ccatcgtttt
     3781 tgaaagcgac ctcgatgtcg ccggcctgtc cgcgtccgtg aatcgtcgcc aggatgttct
     3841 cgcgccgggt ttcgatccac tttacgggct tgttgagctg catcgagatc cacccgagca
     3901 acgcttcttc cgcgtagacg ttgagtttcg aaccaaagcc gccgccgact tcgggtgtga
     3961 tgacgcgcaa cttgttttcc ggcatgccga tcatgagagc gacttgcgta cgcattaagt
     4021 gcgggatctg cgtcgatgac cagagtgtca gttcctcttc gccggggaag tagcgcgcga
     4081 gcacgccgcg tggctccatc gcgatgggtg cgagtcgttt gtgcagtacg cgttgtttga
     4141 gggtgcgatc ggcagacgcg aaggcggcgt cgatgtcacc ctcgcccgcg gtcatcttgt
     4201 aggcgatgtt gtcgccgaac gattcgtgaa tgactgtgcc gccggccgca gcctcttcga
     4261 cgtctgtcac gacgggcagg tcctggtaat caacggccac gagatcgacg gcgtctcgcg
     4321 cagcgtagcg gtcggttgcg acgatcgcgg ctatcggatg gcccacaaag taaaccttgt
     4381 cggtgcccag cacccggtga tccggcactt tgagctccgg caaggcgctg gcgcagggaa
     4441 ctggtccaat cttctctgcg acgtctttgc ctgtgtagac agcaacgaca ccgggtacgg
     4501 cgagcgctgc cgaagtgtcg atgctgttga tgcgggcgtg cgcgtaaaca gagcgcacga
     4561 acgcgacgtg caacgtaccc ggcaggctga tgtcgtcgac gtaatgggcc aggcccttaa
     4621 tcaggcgcgg atcttcagtg cgtcttactc gttggccgac gtaactgttt ggcatgttcg
     4681 tttctccaac taaactttag ccacggatta cacggactta caccaatgca gtttctgtat
     4741 ccgtgttctc agtgtaaatc tgtggcctca tgcagaagcc ttcttggcag cgtactcgac
     4801 ggcttcgata atgtgctggt acccggtgca gcgacacaga ttgccgtcga ggccgtgaca
     4861 gatctcttcg cgcgacgggt ttgcgttgcg atcgagtagt tgcgctgcgc tcatgatcat
     4921 tccgggggtg cagtagccgc actggagccc gtgacgttcc cagaagcctt cctgaaccgg
     4981 atggagttcg ccgttcgtag cgagaccttc gatggtcttg attgacgcac cctcggcttg
     5041 aacggcaaag agcgtgcacg attttactgt ctgtccatcg agcattatcg tgcaggcgcc
     5101 acacaaagat gtttcgcacc cgacgtgcgt tccggtcagg cccagaacgt cgcgcaggta
     5161 gtggacgagg agcatgcgtg gttcgacttc gtcagtgcga gagacaccat tcacgctcat
     5221 attgatagcc tttttcataa gctacctcac ttttgatggt tggttggaaa gaattgtacc
     5281 cacagattac acagattaaa agaacttaga ctgtggcctt gttaatctgc cgtttaagcc
     5341 ggtggatggt tcgtcgcaca actttgagct gagcgtgatc gtgcgcggcc agcgcggcgt
     5401 cgcgttgcgt cttcaaatcg cgaatacgcg atttgattgc gctcttgtcg ataccgacga
     5461 catcgtgatg ttcgtgttga ataccgagat ctttggccaa tgcaccgatc aggtgttctt
     5521 tattcatttg cgtgtagccc tgaacggctt catgttcgct gcctttggcg atctcgcgga
     5581 gctcggcaag agtcatgtgt ttcagttgtt cgtaggtgta cgccataaac gtctccaaat
     5641 ctggtccaag tgttgaagat tggtggccgt gatgatatcc gatgccgttc tctgggggaa
     5701 ctccccccgc tgaaacttcg tttagcccgc tatgcgcggc ctatcgagat ttgaggtcgg
     5761 aatcagggcg aaccgcccgc cgcgatccgt gcatcccaag tcccgaatga aacgattact
     5821 taagctttca actgttttca ttattgctct attgctgctg accctcaccg gcctcggcgg
     5881 agcaatcgtc gacaagctca ccttctttgg gcgggtggtc cggctttaca cgcaagagcc
     5941 ggatcgcact atcgccatgc cgttgaaaga cgtctccaaa agatacatcg cagatacatg
     6001 gcatgcgccg cgcggcacgg gccgattgca tgaaggtcag gacatctttg cgccgaaagg
     6061 aactccggta ctctccgcga caagcggcta cgtctataac atcggcgaga acaatctcgg
     6121 cggccagaca gtttct
//
LOCUS       DASTEY010000555         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1199178, whole genome shotgun sequence.
ACCESSION   DASTEY010000555 DASTEY010000000
VERSION     DASTEY010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199178"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFO72_09755"
     CDS             complement(<1..190)
                     /locus_tag="VFO72_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479619.1"
                     /translation="MPTYVFKGRNRLNEIVVGERVADNREALRQILRREQVTLTSVKE
                     KGREIGIPKIGGRKKVKSK"
     gene            complement(210..1391)
                     /locus_tag="VFO72_09760"
     CDS             complement(210..1391)
                     /locus_tag="VFO72_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET9479620.1"
                     /translation="MRLNETVPQISLSELLRKLSELGGSDLHITTGSPPQVRVDGHLR
                     PLEPYRVLTSADTKQLAYSVLTDAQKHRFEENLELDFSFGVKGLSRFRANLFNQRGAV
                     GAVFRAIPYEIKAFEELGLPPVVKELCNKPRGLILVTGPTGSGKSTTLAAMIDKINRD
                     RHEHILTIEDPIEFLHNHKGCLINQREVNADTHGFAAALRTALRQDPDVVLVGEMRDL
                     ETIESALRIAETGHLTLATLHTNSAASTINRIIDVFPSDQQAQIRAQLSLVLEGILCQ
                     ALLPRAEGSGRAMALEILVPNGAIRNLIREDKVHQIYSMMQTGQDKYGMQTFNQSLAT
                     LYHKRQISLDTALQRSSNVDELRELIDRGSGVNTGQTAKSHASPYAQARPIGTRPNVP
                     R"
     gene            complement(1411..2292)
                     /locus_tag="VFO72_09765"
     CDS             complement(1411..2292)
                     /locus_tag="VFO72_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479621.1"
                     /translation="MLRTIAISLILVLSVGVMLPFSNSAHGVRQSVQVKQKRHYRYRS
                     RAWWRRYRARLRKKRAAAAALAAHRNTMLALPKNIPVGDLSRVGGPSVPVMPENSAGV
                     NSLPAPPAYAVSNTQPVAITSNAQPVIVTANVQAAHAASNGQTRPRVVGNLNAAVKAP
                     AAKLPGQMSLAVVALSRPNPVFLTSREAKNMLAGVAVADLRRIVIDKMVTAGGWVTND
                     FIREVNGKRVFVVMARTPGDTLAPERAWTFYFTEAGGRIYGLTTDSPLEFADRMSNEA
                     ERYIETLRAKAEPISNK"
     gene            complement(2311..>2559)
                     /locus_tag="VFO72_09770"
     CDS             complement(2311..>2559)
                     /locus_tag="VFO72_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET9479622.1"
                     /translation="IKTYKGKGCSTCNNTGYKGRIGLYEVMEITDELRELILIGASAL
                     ELRKKAIEDGMITLRESGLCKIRDGVTTLEEVVRETVA"
BASE COUNT          513 a          724 c          732 g          590 t
ORIGIN      
        1 ctttcgactt gaccttcttg cgcccgccaa ttttcgggat gccaatctcg cgtccctttt
       61 ctttgactga cgtaagcgta acttgttcac gccgcaagat ctggcgcagc gcttcgcgat
      121 tgtcggcaac gcgctcgccg acaacgatct cgttcaagcg gttgcggccc ttaaaaacgt
      181 acgttggcat gactatcagc tccagtggct tatctgggta cgttcggtct cgtaccaatt
      241 ggtcgcgctt gagcatacgg actggcgtgc gactttgcgg tctgcccggt atttacaccg
      301 gaaccgcggt cgatcaattc gcgcagttcg tccacgttcg acgaacgttg caacgccgtg
      361 tccaaactga tctgccgctt gtgatacagc gtcgccagcg attgattgaa cgtctgcatg
      421 ccgtacttgt cctggcccgt ctgcatcatc gagtagatct ggtggacttt gtcttcgcga
      481 atgaggttac gaatcgcgcc gttgggcacc agaatttcga gcgccatggc ccggccggat
      541 ccttcggcac gcggcagcag tgcctgacag agaattcctt cgagcactaa cgagagttgt
      601 gcgcgaatct gcgcctgctg atcggaaggg aagacgtcga tgatacggtt gatggtcgaa
      661 gccgcggagt tggtgtgcaa cgtcgcgagc gtaaggtggc ccgtctccgc gatacgcaaa
      721 gctgactcaa tcgtctcgag gtcgcgcatt tcaccgacga gcaccacgtc aggatcctgg
      781 cgcaacgccg tgcgaagagc agccgcaaac ccgtgcgtgt cggcgtttac ttcgcgctgg
      841 ttgatcagac agcctttgtg gttgtgcaaa aattcgattg gatcctcgat cgtcagaata
      901 tgctcgtgac gatcgcgatt gatcttatcg atcatggctg ccagcgtggt tgacttgccg
      961 gaaccggttg gaccagtcac gagaatcagg ccgcgcggct tgttgcacag ctcttttacg
     1021 accggcggca agccgagctc ttcgaaagcc ttgatctcgt aaggaatggc gcgaaagact
     1081 gcgcccactg cgccgcgctg gttgaaaaga tttgcgcgga aacgcgacaa gcctttgacg
     1141 ccgaacgaaa aatccagttc gagattctcc tcgaaacgat gcttctgcgc gtcagtcaga
     1201 accgagtagg cgagttgttt cgtgtctgca gacgtcagaa cgcgatacgg ctcgagtgga
     1261 cgaagatgtc cgtccactcg aacctgtggc ggggagccgg tcgtgatgtg aagatccgaa
     1321 ccgccgagtt cagaaagctt cctcagcagt tcggacaagg aaatttgggg cacagtttcg
     1381 ttcagtcgca tctgggtcgt ctctccccgt ttatttgtta ctgattggct cagctttggc
     1441 gcgcagcgtt tcgatgtaac gctctgcctc gttcgacatg cgatcggcaa actcgagcgg
     1501 tgaatcggtg gtcagcccgt aaatgcggcc gcccgcttcc gtgaaataaa acgtccacgc
     1561 tctttccgga gccagcgtgt cgcccggtgt gcgagccatg acgacaaaca cacgcttgcc
     1621 attcacttcg cgtatgaagt cgttagtgac ccagccgcct gccgtaacca tcttgtcgat
     1681 tacgatccgg cggaggtcgg caacggccac gcccgccagc atgttcttcg cttcacgcga
     1741 agttaaaaac accggattag gccggctgag tgcaacaact gcgaggctca tttggccggg
     1801 cagtttcgcc gcaggcgctt tgacagccgc gttcaagtta ccgacgacgc gcggtctcgt
     1861 ttggccattg gaagcagcat gggccgcctg tacgttggcg gtaacgatga ccggttgagc
     1921 gttcgaagtg atggcgactg gttgtgtatt ggagacggcg tatgccggtg gcgccggcaa
     1981 tgagttcacg ccggcggaat tctcgggcat gaccggcact gatggaccac caacgcggga
     2041 tagatcgcca accggaatgt ttttcggtag cgccaacatc gtgtttcggt gggccgccaa
     2101 tgccgccgcg gctgctcgct tcttgcgcag acgggcgcgg tatcgccgcc accatgcacg
     2161 cgagcgatag cgataatgac gcttctgttt gacctgtaca ctttgccgga cgccatgcgc
     2221 ggagttgctg aacggcaaca tcacgccaac ggacagcaca aggattagcg atatggcaat
     2281 tgttcttaac atgctcgggt tctcctcgaa ttaggcgacc gtctcgcgaa caacttcctc
     2341 aagcgtggtc acgccgtcgc gaatcttgca aaggcccgat tcacgcaacg tgatcattcc
     2401 gtcttcgatc gcctttttcc tcaattcaag tgcggaagca ccaatcaaaa tcaactcacg
     2461 aagttcgtcg gtgatttcca tcacttcgta aagaccgatt cggcccttgt agccggtgtt
     2521 gttgcaggtc gaacatccct tgcctttgta cgtcttgat
//
LOCUS       DASTEY010000556         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_358478, whole genome shotgun sequence.
ACCESSION   DASTEY010000556 DASTEY010000000
VERSION     DASTEY010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358478"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFO72_09775"
     CDS             complement(<1..227)
                     /locus_tag="VFO72_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479623.1"
                     /translation="MLTEKRRYSLQIWLLGLGFYLFYTPYSGLTKALSSGLLPGANGA
                     VKGVVLLPVSAIATVVGMLGFISVMGWWKYAG"
     gene            complement(236..1468)
                     /locus_tag="VFO72_09780"
     CDS             complement(236..1468)
                     /locus_tag="VFO72_09780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9479624.1"
                     /translation="MTTTPAAINHSYELLDLSAEQQELINRVAVLARENFAARAAEYD
                     RTATFPAEDFDDLFRAGLHAPVIPREHGGLGLGPYSNDVFTLWMMTKEIARADLSLAR
                     CWEGHVNSLVLLDGMSAEEQKTRWFKGVVERGEKWVAWSGEPQAQKPGETVRFGTTVE
                     RVENGYVINGNKAFATSAGGAEWAILLVNTAGPGGVRHAGAAMLETQLMMACNLSDPT
                     IEIDASWWDPIGMRATVSHLVHFRRTFIPDSDVIGYPGQYLKEGWQTCFIPHYAATFL
                     GAAEAAFDYALSYIKTQKKTEDPYVQHHIGQMSVNVETAHIWLKHVAHLWETGRYLEA
                     QIAGSRARHVVEHLAEDTVKQCIRACGARSLIRPSVLERIYRDLSFYVRHDNDDQILA
                     TIGKSVLGLTHDPSFYKP"
     gene            complement(1718..>2200)
                     /locus_tag="VFO72_09785"
     CDS             complement(1718..>2200)
                     /locus_tag="VFO72_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9479625.1"
                     /translation="DTTDPDVVYAAGNQPDVHLREKRAAVIGCGGAGRAIAAALAESG
                     AGVTLINRGAERGERASSLLGLPYMPLLNFDVKGYDIVVNATPVGRDTDEVPFALERL
                     SNNAVVIDLVYGSRPTPLVDSTLARRQIVIDGRDVLFTQVRNQFRLMTGKEMPLTTVK
                     "
BASE COUNT          482 a          633 c          608 g          477 t
ORIGIN      
        1 ccggcatact tccaccagcc catgacgctt atgaaaccca acatgccaac tacggttgcg
       61 attgccgaaa caggcaacag caccacacct ttgacagcgc cgtttgcgcc cggcaggaga
      121 ccactcgaaa gggctttcgt cagaccgctg taaggtgtgt agaagagata aaagcccagg
      181 cccagcagcc agatttgcaa actgtagcgg cgcttttcgg tcagcatgtc ggtgcttacg
      241 gtttgtagaa agacggatcg tgcgtcaggc ccaacaccga tttaccaatc gtcgcgagga
      301 tctgatcgtc gttgtcgtga cggacataga aagagagatc tcgataaata cgttcgagca
      361 cgctcggccg gatcaaagag cgtgcgccgc acgcgcgaat gcattgcttg acagtgtctt
      421 ccgccaaatg ttcgaccacg tgacgcgcgc ggctgcccgc gatctgcgcc tcgagataac
      481 gccctgtctc ccacagatgc gccacgtgct tcaaccaaat atgcgcggtt tctacattca
      541 ctgacatctg cccgatatga tgttgcacgt aaggatcttc ggtcttcttc tgcgttttga
      601 tgtatgagag cgcgtaatca aacgcagctt cagcggcgcc gagaaaagta gcggcgtaat
      661 gtggaataaa acaggtctgc cagccttctt tgagatactg gccgggataa ccgattacat
      721 cggaatcggg aatgaacgtg cgccggaagt ggaccaggtg actgacggtg gcgcgcatgc
      781 caatcggatc ccaccacgac gcgtcgatct cgattgtcgg atcggaaagg ttgcacgcca
      841 tcatgagctg cgtttcgagc atcgcggcgc cggcgtgacg cactccgccg ggtccggcag
      901 tgtttacgag cagtatggcc cactcagcgc cgcccgcact ggtagcaaac gccttgttgc
      961 cattgatgac gtagccgttt tcgacacgtt ccacagtcgt gccaaatcgc accgtttcgc
     1021 ccggtttctg cgcttgcggt tcgccactcc atgcgaccca tttctctcca cgttcgacaa
     1081 cgcctttaaa ccaacgcgtt ttctgttctt cagcgctcat accgtcgagc agtacgagcg
     1141 agttaacgtg cccttcccag caacgcgcga gtgacagatc ggcgcgagcg atctccttgg
     1201 tcatcatcca caaggtgaag acgtcgttgc tgtatggtcc aagcccgagc ccgccgtgtt
     1261 cgcgcggaat gacgggcgca tgcagaccgg cgcgaaacag gtcgtcgaag tcttcggcag
     1321 gaaacgtcgc cgttcgatcg tactcggcgg cacgcgcagc aaagttctct cgcgcgagca
     1381 cggcgacgcg attgatcagt tcctgctgct cggcactcag gtcgagtagc tcgtaagagt
     1441 ggttgattgc tgcgggcgta gtggtcatct ctatttgact gggtttgccc aagaagcttt
     1501 ctatttgtac ctaaggtcct gctccggagg agcaaaatat ttatagctct tcggtttctc
     1561 aattacaccg ctccgtcagg agcggtatgt aaactgaacg agcacattgc gctccgctgg
     1621 agcgcaccag ctaatgaatg taagggctat aaacatcgtg ctcctccgga gcactcgcca
     1681 gcagaagcat taagtaagat cacttttcgg gcaaagccta tttgactgtt gttaagggca
     1741 tttcttttcc agtcattaaa cgaaactgat tgcgcacttg cgtaaacagc acgtcacggc
     1801 cgtcgatcac gatctgacgt cgcgccagtg tgctatcgac gagtggtgtc ggacgcgagc
     1861 cgtagacgag atcaatcaca acagcattgt tactcaagcg ctcgagtgca aacggaactt
     1921 catctgtgtc gcgaccgaca ggcgttgcat tgacgacgat gtcataacct ttgacgtcga
     1981 agtttagcaa cggcatgtac ggcaggccga gcagtgaaga ggcgcgttcg ccacgctctg
     2041 caccacggtt gatcaacgtg acaccggcgc ccgattcggc gagtgccgcc gcgatcgctc
     2101 ggcccgcgcc gccacagcca atcacggcgg cgcgcttttc gcgcaggtgt acatccggct
     2161 gattgccggc agcatagact acgtcgggat ccgtcgtatc
//
LOCUS       DASTEY010000557         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1446740, whole genome shotgun sequence.
ACCESSION   DASTEY010000557 DASTEY010000000
VERSION     DASTEY010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446740"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="VFO72_09790"
     CDS             complement(<1..1736)
                     /locus_tag="VFO72_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HET9479626.1"
                     /translation="MKTTRYVLLTLIVLICGPLTVAQQHNLMPVPASVTFNEDRLAVN
                     ESFKVAIRGHSDERLRAAVARFVKRLEGRTVLSFAPGLALDDQTTTLIVHCQGPGKEI
                     PALNENESYRIDITYRQALLSAPTVVGAIRGLETMLQLLESDRRGYFFSGLTIQDQPR
                     FPWRGLLIDVARHFEPVEVLKRNLDAMAALKMNVFHWHLTEDQGFRVESKKFPKLHQL
                     GSDGNYYTQDQIRDVIAYARDRGIRVVPEFDIPGHSTSWLVGYPELGSAPGPYTIERR
                     PGIFEPALDPTREEVYKFLDGFLSEMAALFPDAYMHIGGDENEGKQWDRNPAIQAFMK
                     AKGIKDNHALQAYFNTRLLKILQKNHKKMIGWDEILQPELPKDIVIHSWRGTAALAEA
                     ARKGYDGILSNGYYIDLIQPASQHYIADPLPADSTLTPDEAKHVLGGEATMWSEWVTH
                     ETIDSRIWPRTAAIAERLWSPRHITDVNDMYRRLAAISLQLEELGLTHKKNQDMMLRR
                     LLRSDDIGPLRTLASIVEPVKEYRRYQQRPQTMLSPLTGLVDAALPDSEAARQFASMV
                     DAFLADAPRFQL"
     gene            complement(1733..>2024)
                     /locus_tag="VFO72_09795"
     CDS             complement(1733..>2024)
                     /locus_tag="VFO72_09795"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HET9479627.1"
                     /translation="DKAELGVTVTVTNTGQRAGKEVVLVYVSDLVASLSPPNRRLRRF
                     AKVSLEPNQSRTLTFKLRREDLSFIGADNKPVVEPGEFEVKIGNLSQRFTLR"
BASE COUNT          410 a          562 c          576 g          476 t
ORIGIN      
        1 tagagttgaa accgcggcgc gtccgcaaga aacgcgtcaa ccatcgacgc aaactggcgc
       61 gccgcttcgc tgtcaggtag tgcagcgtca accaatccag tcaaagggct cagcatcgtt
      121 tgcggtcgtt gctgataacg ccggtactcc ttcacgggtt cgacgattga agcgagtgtt
      181 cgcagtggtc caatgtcgtc actgcgtaac aaacgtcgca gcatcatgtc ctggttcttt
      241 ttgtgcgtca ggcctaactc ttccaactgc aaactgattg ccgcgaggcg gcgatacatg
      301 tcgttcacgt ccgtgatgtg tcgcggcgac cacagtcttt ccgcgatcgc ggccgtgcgt
      361 ggccagatac gcgaatcgat ggtctcgtgc gtcacccact cgctccacat ggttgcttcg
      421 ccgccgagta cgtgcttcgc ttcgtcgggt gtcagcgtgc tgtcggctgg aagcggatcg
      481 gcgatgtagt gttgcgaagc tggttggatt aaatcgatgt agtaaccgtt cgacagtatg
      541 ccgtcgtagc ccttgcgcgc cgcttcagcg agtgcggccg tgccgcgcca ggagtggatc
      601 acgatgtctt tcggcaactc cggttgcagg atctcgtccc agccgatcat cttcttgtgg
      661 ttcttctgaa gaatcttgag cagccgcgtg ttgaaataag cctgcaacgc atggttgtct
      721 ttgatgcctt tggccttcat gaaagcctgg atcgcgggat tgcggtccca ctgcttgcct
      781 tcgttttcgt cgcccccgat gtgcatgtaa gcatcgggaa acagcgctgc catctcactg
      841 agaaagccat cgagaaactt gtagacttcc tcgcgcgtcg gatcgagtgc gggttcaaag
      901 atgccaggac gacgttcgat cgtgtatgga ccaggcgcgc tgccgagttc cggataaccg
      961 accagccagc tcgtactgtg ccctggtatg tcaaactcgg gcacgacgcg aatgccacga
     1021 tcgcgagcgt acgcgatgac gtctcttatt tgatcctgcg tgtagtagtt gccgtccgag
     1081 ccaagctggt gcagttttgg aaacttcttg ctttcgacgc ggaagccctg gtcttccgtg
     1141 aggtgccagt gaaagacgtt catcttcaac gccgccatcg cgtcgagatt gcgtttgagc
     1201 acttcgaccg gttcgaagtg acgcgcgacg tcgatcaaca aaccgcgcca gggaaagcgt
     1261 ggctggtcct gaattgtgag gccgctgaag aagtaaccac gccgatcgct ttcgagcaac
     1321 tggagcatgg tctcgagtcc gcgaatagcg ccaacgacgg taggcgcgct caacaacgcc
     1381 tgacggtagg tgatgtctat acgataactc tcgttttcgt tcagcgcggg aatctctttg
     1441 cccggccctt gacaatgaac gattaacgtt gtggtttgat cgtcgagtgc gagacccgga
     1501 gcgaacgaga gcacggtgcg gccttcaaga cgtttgacaa aacgcgcgac cgctgctcgc
     1561 aaacgctcgt ctgaatgtcc gcggatggcg actttgaatg attcgttaac tgctagccga
     1621 tcttcattga acgttacaga cgccggcacc ggcatcaggt tatgttgttg ggccaccgta
     1681 agtggaccac agatcagaac aatcagtgtt aaaagaacgt atcgagtggt tttcatctca
     1741 atgtgaacct ttgcgatagg ttgccaatct tcacttcaaa ctcgcccggc tcgaccacgg
     1801 gcttgttgtc cgccccgata aacgagagat cctcgcgtcg cagtttgaag gtcaaggtac
     1861 ggctctggtt cggctcgaga ctgaccttcg caaatcgtcg cagccttcgg ttcggtggtg
     1921 aaagtgacgc aaccagatcg ctgacgtaga ccagcacaac ttctttacca gcgcgctggc
     1981 ccgtgtttgt aacggttacg gtgaccccaa gctcagcctt atcc
//
LOCUS       DASTEY010000558         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_886478, whole genome shotgun sequence.
ACCESSION   DASTEY010000558 DASTEY010000000
VERSION     DASTEY010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886478"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2265
                     /locus_tag="VFO72_09800"
     CDS             <1..>2265
                     /locus_tag="VFO72_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9479628.1"
                     /translation="DLPLTAEQPIVQKLDTDAAPVVRVAVSAPRSLREVTDVADNQIK
                     QQIESINGVGDVQIVGGRRREIQIWVDPDKMRAFDVTVAQVADAVRAQNMEVPGGRVE
                     EGARELTVRTMGRIVDPVQFNNLVVANRGTYSVKLSDIGYAEDGAEEPRTEARLNGQP
                     AVTLIVSKQSGQNTVAIADAVKERLQEISKTLPPGFKTEVVGDQSIFIKASIEAIQTH
                     LIEGTILASIVVFVFLWSYRSTVIAGLAIPTSIIATFGLMAAMGFTLNNITMLALTLM
                     VGIVIDDAIVVLENIFRFIEEKGVPPFQAAIEGTKEIGLAVMATTLSLLAVFLPVGFM
                     GGIVGRFMSSFGLTSAFAIAVSLLVSFTLTPMLAARLIKRSDDKKERGLERNTEPADH
                     GSKQSRFYRPIDRNYTKLLTWSMAHRWAIVLACVAVIASIIPLFMFVGKNFLPVDDQS
                     QFEIQVRTPEGSTLAATSTLAERISVDLRKFPGVTDTLTTIGGGQQEQVNLASIYVKL
                     TPLEERDLSQEKLMVRARNEVLAKYLQQYPGQLRTSVQQVAAISGGGFRNADIQYVIG
                     GPDLKKLTEYSDKLLEKMRTIPDVVDADSTLISGKPELRVVIDRNRAADLGVRVGDIA
                     QALNSLVAGQKVSTFNAGTDQYDVRVRSVGEFRTSAEGLQRMIVSSTKVGWVSLDNLV
                     RVEEGTGPSAIDRLNRQRQVMLLGNVRPGGSQAAVIDQLNQFAQEIGIDPAYSTGLAG
                     RSKELGRAGYYFGLA"
BASE COUNT          481 a          627 c          687 g          470 t
ORIGIN      
        1 gatcttccgt tgacggccga gcagccgatc gtgcagaagc tcgatactga cgcagcgccc
       61 gtcgtgcgcg tcgcggtgtc agcgccgcgt tcgctgcgcg aagtgacgga cgtcgccgac
      121 aaccagatca agcagcagat cgaatccatc aacggcgtcg gtgacgtgca gatcgtcggt
      181 ggccggcgtc gcgagattca gatttgggtc gaccccgaca agatgcgcgc gttcgacgtg
      241 actgtggcgc aagttgccga tgcagtgcgg gcgcagaaca tggaagttcc gggtggccgt
      301 gtcgaagaag gcgcgcgcga actgaccgtg cggacgatgg gccgtatcgt cgatccggtg
      361 cagttcaaca atctcgtcgt agcgaatcgc ggcacttatt cagtcaaact cagcgacatc
      421 ggttacgcgg aagacggagc ggaagagcca cgcacggaag cgcgtttgaa cggtcaaccg
      481 gcagtaacgc tgatcgtctc gaaacagtcc ggacagaaca ccgtcgccat cgctgacgct
      541 gtcaaggagc gtttgcaaga gatcagtaag acactgccgc ccggcttcaa aacggaagtc
      601 gtcggcgatc agtcgatctt catcaaggcc tcgatcgaag cgattcagac gcacttgatc
      661 gagggcacca ttctcgcctc gattgtggtg tttgtgttct tgtggagcta ccgttccacc
      721 gtcattgccg gactcgcgat tccgacttca atcatcgcga cgtttggttt gatggccgcg
      781 atgggcttca cgctgaacaa catcacgatg ctcgcgctga cgctgatggt cggcatcgtt
      841 attgacgacg ccattgtcgt gctcgaaaac atctttcgct ttattgaaga aaagggtgtg
      901 cctccgttcc aggcggcgat tgaaggaacg aaagagatcg gcctcgcggt gatggcgacc
      961 acactttcac tgctggcggt gtttctgccg gtcggcttca tgggcggaat cgtcggccgc
     1021 ttcatgtcat cgtttggttt gacctcagcg tttgcaatcg cggtgtcgtt gctggtgtcg
     1081 tttacgttga cgccgatgct ggcggcccgc ttgattaagc gaagcgacga caaaaaagaa
     1141 cgcggactcg aaagaaacac cgaacctgcc gatcacggtt cgaagcaatc gcggttctat
     1201 cgtccgatcg atcgcaacta caccaagttg ttgacctggt cgatggctca ccgctgggcc
     1261 atcgtgctgg cgtgcgtcgc cgtgattgcc agcatcattc cgctcttcat gtttgtgggt
     1321 aagaacttcc tgccggtcga cgatcagtcg cagtttgaga tccaggtacg cactccggag
     1381 ggctcaacgc tcgcggccac gtcaaccctg gccgagcgta tttccgtcga tctgagaaaa
     1441 tttccgggcg tcacagacac gttgacaacc atcggcggcg gacagcagga acaggtcaac
     1501 ctcgcgtcga tctacgtaaa actcacgccg ctcgaagaac gcgacctatc gcaagaaaaa
     1561 ctgatggtgc gtgcgcgtaa cgaagtcctg gcgaagtact tgcagcagta tccgggacaa
     1621 ctacgcacca gcgtgcaaca agtcgcggcg atctccggcg gcggtttccg caacgcggat
     1681 attcagtacg tcatcggtgg tccggatcta aagaaactga cggaatactc agacaagttg
     1741 ctcgagaaaa tgaggacgat tcctgacgtc gtcgacgctg actcaacgct gatcagcggt
     1801 aaacctgaat tgcgtgtcgt gatcgatcgc aatcgcgctg ccgatttggg tgtgagagtc
     1861 ggagacattg cgcaggcact caactcgctc gtggccggcc aaaaagtctc gaccttcaac
     1921 gcgggaacgg atcaatacga cgtgcgcgtc cgctcggtcg gcgagttccg cacgagcgcc
     1981 gaaggtttac agcgaatgat cgtttcctcg acgaaggtcg gctgggtaag tctcgacaac
     2041 ctggtgcgcg ttgaagaagg cactggacca tcggcgatcg accgcttgaa ccggcagcgg
     2101 caggtgatgt tgcttggcaa cgtgagaccc ggcggctcgc aggcggctgt tatcgatcaa
     2161 ctgaatcagt ttgcgcagga gattgggatc gatcccgcct actcgactgg tttggccggt
     2221 cgctcgaagg agctcgggcg cgctggttat tactttggac tcgcg
//
LOCUS       DASTEY010000559         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_474126, whole genome shotgun sequence.
ACCESSION   DASTEY010000559 DASTEY010000000
VERSION     DASTEY010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474126"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VFO72_09805"
     CDS             complement(<1..1156)
                     /locus_tag="VFO72_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET9479629.1"
                     /translation="MQPTPSASYSLTLRVRLSSRAGSLGELTTAIGKAGGDIGAIDIV
                     NVGNDFIIRDITVSSVSSTHGEQIVETVKDVDGVELLQVSDPTFLMHLGGKIEVVPKV
                     PLKTRADLSMAYTPGVARICDAIYKDPDKAFTLTIKKNTVAIVTDGTAVLGLGDIGPA
                     AALPVMEGKAMLFKEFAGVDAFPICLNTKDPDEIVRTVKAISTVFGGINLEDISAPRC
                     FQIEERLREELDIPVFHDDQHGTAVVVLAALINALKLVGKDISDVKVIVNGVGAAGVA
                     CSKIIMAAGVRNIVGCDQTGALYRGRRENMNWMKEWYAQNTNPGEEKGTVHEVIKGAD
                     VFLGLSVPGVIDVEDVKAMAQKPIVFAMANPVPEIMPEEAAPYVAVMATGR"
     gene            complement(1164..2036)
                     /locus_tag="VFO72_09810"
     CDS             complement(1164..2036)
                     /locus_tag="VFO72_09810"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HET9479630.1"
                     /translation="MPDRKRKQKPKVSIIGSGRLGTALAVALAGRGYSIGSLVARRAQ
                     KARQAASLLDAEVQVLAAKQLPSLRPADVFLITVPDDQIAGVAGALSSLSFTATALHT
                     SGALSAEVLAPLRERGWHIGSVHPLMAVSDAREGDGALRGGYWSVEGDKTTLRLAKTI
                     VSDLSGKSFSIRSEDKPLYHAAAVMAAGNVVALFDVALEMLVQCGLTRKTARSVLLPL
                     IASTIHNLETKDPAQALTGTFARGDVETVKRHLAALKNNDLADALELYRLLGERSLKL
                     TKKHPQITQILKSV"
     gene            2228..3415
                     /locus_tag="VFO72_09815"
     CDS             2228..3415
                     /locus_tag="VFO72_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9479631.1"
                     /translation="MNNFSRATSAFVLTASLLVSSVAAVAQQAPATKPAEDPQKTNQT
                     QAEQKKTTPAATTAKSNKPLSTNEDPNMIGKRNINTGIIAKMSGSTEKEVRLGRELAA
                     EVDRQAKFVDDPIITEYVNRVGQNIVLHSDAKVPFTIKVIDSDEVNAFALPGGFFYVN
                     KGLILAADNEAELAGVMAHEIAHVAARHAVENQTKGSLLEYAALAGSIFIGGIPGMIY
                     QNTAGIGLLGIFMKFSRGAEEEADKLGVQYMYAAGYDPGAMATMFEKLEAKNKKKPGF
                     ISRAFASHPAPPDRRQAALALAARFPEIEEYVISSSEFQRVKGRLLRLSNARASTAGA
                     IATGDESGAPGRPTLKRRQPTPDDTTTTTPAEGGTEQPKSTEAPPKLKRNDTGTTKTP
                     DKP"
BASE COUNT          794 a         1018 c          936 g          709 t
ORIGIN      
        1 aacgacccgt agccatcacc gcgacgtacg gcgccgcctc ctcgggcatg atctcaggaa
       61 ccggattcgc catcgcaaac acgatcggct tttgggccat cgccttcacg tcttccacat
      121 cgatcactcc gggcactgac aaccccagaa acacgtcggc gcctttaatg acctcgtgaa
      181 ctgttccctt ctcctcaccg gggtttgtgt tctgcgcgta ccactctttc atccagttca
      241 tattctcgcg gcgcccacga tagagcgctc cggtctgatc gcaaccgacg atattgcgga
      301 cgcccgcggc cattattatc ttcgagcacg ccacacccgc cgcgccgacg ccgttgacaa
      361 taaccttgac gtcgcttatg tccttgccca ctaacttcaa cgcattaatc agtgccgcga
      421 gcacgacgac tgcggtgccg tgctgatcat cgtgaaaaac cgggatgtcg agctcttccc
      481 tcagtcgttc ttcgatctgg aagcaacgag gcgcggaaat atcttctaga ttgatcccac
      541 caaaaactgt cgagatggct ttcactgttc gcacgatctc gtccggatct ttcgtgttca
      601 aacagatcgg gaaagcatcg acgcccgcga actccttgaa cagcatcgcc ttcccttcca
      661 tcaccggcaa cgccgccgct ggtccaatgt cgcccagacc gagcactgcg gttccgtcag
      721 ttacgatagc gactgtgttt ttcttgatcg tcaaggtgaa ggctttatcg ggatctttgt
      781 aaattgcgtc gcagatgcga gcaacgcccg gcgtgtaagc catcgagaga tcggcgcgcg
      841 tcttcagcgg caccttcgga acgacctcga tttttccgcc caaatgcatc aggaacgtcg
      901 ggtctgacac ctgcagcaac tcgacgccat cgacgtcttt aactgtttct acaatctgtt
      961 cgccgtgtgt actggaaacg gagctgacag tgatgtcgcg aatgatgaaa tcgttaccaa
     1021 cgttgacgat gtcgatggcg ccgatgtcgc caccagcctt gcctattgca gtggttaact
     1081 caccaagcga tccggcacgt gaagaaagcc tgacgcgcaa ggtcaaactg taactcgcgc
     1141 tgggtgttgg ttgcatagcg tgattatacc gatttgagaa tctgtgtaat ctgtggatgc
     1201 ttctttgtaa gtttcaggga tctctcgccg agcaaacgat aaagctcgag cgcatccgcg
     1261 agatcgttat tcttgagcgc cgcgagatgt cgcttcactg tttccacgtc accgcgggca
     1321 aaggtgcccg ttaacgcctg cgccgggtct ttagtttcga gattgtgtat ggtgctggca
     1381 atcaagggca gcaacacgct gcgagcggtc ttccgtgtca gcccgcactg aaccagcatc
     1441 tcaagcgcca catcaaacaa ggcaacgaca ttacctgcag ccataaccgc ggccgcgtga
     1501 taaagcggtt tatcttccga gcggatcgaa aagcttttgc cggataggtc gctgacgatt
     1561 gttttcgcca ggcgcaaggt ggttttgtca ccctcgacac tccagtaacc accgcgcagc
     1621 gctccgtcac cctcgcgcgc gtcgctcaca gccattagcg ggtgaactga accgatgtgc
     1681 caccctcgtt ctcgcaaagg ggcaagcacc tccgctgaca gtgcgccgct ggtatgaagc
     1741 gcagtggctg tgaaactaag actactcagc gcaccagcaa cacccgcaat ctgatcgtcc
     1801 ggaacagtga tcaaaaagac gtcggcaggc cgcaatgaag gcaattgttt tgcggctaag
     1861 acctgcacct ctgcgtctaa aaggctagcg gcttgccgcg ccttttgcgc ccggcgggcg
     1921 acgagggacc cgattgaata tcctcgaccg gcaagcgcga ccgccagggc ggttccgagg
     1981 cgtccgctgc cgatgattga gactttcggc ttttgcttcc ttttcctgtc gggcataggt
     2041 atagttacgg ctaagctaag tgatttcagg cgcgaaagtc agttcccctc cgggccagga
     2101 agttgtattt ggtgaaccaa ccaagccgcc gaacctgtgc atcatagact gcgcgtcagg
     2161 atttagcgaa aacgacagtc gtagacccca ccctcaggtc attcgaaaac tttggaggtt
     2221 ttttgagatg aacaacttct cccgcgccac gagtgctttc gttttaaccg cgtctttact
     2281 ggtgagctcg gtggctgcgg tcgcgcaaca agcgccggcc actaaaccag ccgaagatcc
     2341 tcaaaagact aaccaaaccc aggcggagca gaagaagacg acgcccgcgg cgacgactgc
     2401 gaagtcaaac aaaccgctgt cgaccaacga agatcccaac atgatcggca agcggaacat
     2461 caataccggc atcattgcca agatgagcgg ctccaccgaa aaggaagtgc gcctcggacg
     2521 tgagctggca gcggaagtcg atcggcaagc gaagtttgtt gacgatccga ttatcaccga
     2581 gtacgtgaat cgcgtcggtc agaacatcgt gctccattcc gatgcgaaag tgccattcac
     2641 tatcaaagtc atcgattctg atgaagtgaa tgcgtttgca ctgccgggtg gcttcttcta
     2701 cgtaaacaag gggctgatcc tcgcggctga taacgaagcg gaactggccg gcgttatggc
     2761 gcacgaaatt gcgcacgtcg cagcccgcca cgccgtggag aaccaaacca agggaagttt
     2821 gctcgagtac gctgccctcg cgggatccat attcatcggc ggcattcccg gaatgattta
     2881 ccagaatacg gccggcatcg gactgctcgg gatcttcatg aagtttagcc gtggcgcgga
     2941 agaagaggca gacaagctgg gcgtccagta catgtacgcc gccggttacg atccgggcgc
     3001 gatggccacg atgtttgaaa agctggaagc caagaacaag aaaaaacccg gatttatctc
     3061 gcgcgcattt gcgtcgcacc cggcaccgcc ggaccgcaga caagcggcac tcgctttggc
     3121 agcccggttt ccggagatcg aagagtatgt catcagcagt tccgagtttc agcgggtgaa
     3181 gggccggctg ttaagactgt cgaacgcgcg tgcttcgacg gctggagcga ttgcgacagg
     3241 cgatgaaagt ggagcacccg gcaggccgac attgaagcgt cgccaaccaa caccggacga
     3301 taccaccacg acgactccgg cggaaggcgg caccgagcag ccgaagtcga ctgaggcacc
     3361 gccgaagctg aaaagaaacg acaccgggac taccaaaact cccgacaagc cgtaacaact
     3421 tgtggtaacc tacgagaccg aggcccttcg ggatccc
//
LOCUS       DASTEY010000560         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1364043, whole genome shotgun sequence.
ACCESSION   DASTEY010000560 DASTEY010000000
VERSION     DASTEY010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364043"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..667
                     /locus_tag="VFO72_09820"
     CDS             227..667
                     /locus_tag="VFO72_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479632.1"
                     /translation="MLRGNYTARIDVKGRLKIPTEFRRLIEEKHCKDFYITSLTGDCV
                     RIYPLPEWESIEQRLSLLPSMDPARRKFLDRTNYYGQQATIDGQGRLLIHPLLRKSAE
                     VIGDVAVLGYLTYLEVWELDKFRARLLADPYTEEDEAAIARLGI"
     gene            716..1750
                     /gene="rsmH"
                     /locus_tag="VFO72_09825"
     CDS             716..1750
                     /gene="rsmH"
                     /locus_tag="VFO72_09825"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9479633.1"
                     /translation="MTRNKEQRTKNKKRIFERDHGAVVMAAPLGGIGAPHRPVLLKET
                     LQFLVLERGGLFIDCTVGLGGHSEAILQASERTRVIAMDLDPSALAYSRKRLATYGER
                     FRAYQANFRELADVLSRAGERDPDGILVDLGVSSLQFDSPERGFSFRFDAPLDMRMNP
                     TIGATAADLLQQLPESEIARIIFEFGEERHSRRIARRLVERRELGNPVTTTTELADLV
                     MAAVGGGKRQQIHPATRTFQALRIAVNNELEGLGDFVELAVDLLAPDGRFVGISFHSL
                     EDRILKQALRRLSGHCQCPPRLPVCSCGAREAVEVLTRRPVAPSAREIEENPRARSAK
                     LRAARTVVSR"
     gene            1801..2130
                     /locus_tag="VFO72_09830"
     CDS             1801..2130
                     /locus_tag="VFO72_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET9479634.1"
                     /translation="MKRFPEHQLNAKIRRTRDVTALSRLALLLFCGLVLAGGFVFAAK
                     QHFAAVQYGYQNEHLRSERKRLLEENQRLALDKERVTAPDKLEPAARRLGLKPARPGQ
                     IVTLNHR"
     gene            2170..>4114
                     /locus_tag="VFO72_09835"
     CDS             2170..>4114
                     /locus_tag="VFO72_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9479635.1"
                     /translation="MAVIAKSKSRPDVSLSRALFVAAFIGFWMLIVCARLVHLQFSQH
                     NGLANRARLQQQNAIETTPQRGELLDRHGRQLARSVQTVSLFLDPDGLDVGELECTAQ
                     YLSVSLGMKYKNLLAQFREGQAEKRRFIWIARRLDADHANRIVAMNLPGLQSVLEPKR
                     YYPNGPLAAHVLGYVGLDGDGLGGLERFYNSKISGEPGRLFLERDANGKPYESYEIAS
                     KPGQTVVLTIDQSIQYQAEQALNNAVKSSRARSGTVIVLDPRSGDVLALANAPSFDPN
                     NVSASAAETRSNWALQNIYEPGSTFNVVAFSAALEKKLASVDDHIDCQMGAITVAGRV
                     IHDHKPFGTLTISEALAKSSNVAAIKLGLRVGDETMYDYITRFGFGSKTGIELPGETA
                     GLLRKVERWQPSSIGSIAIGQEIGVTPVQMAAAFGALANDGVRVAPHLIREVRNEAGA
                     VIYQPQPEQRRVINAETAIALRGMLEGVTLRGTAKQAQLDGYSAAGKTGTAQKIDPKT
                     RAYSATKFVASFVGFAPVSNPQIVIVVVIDEPSGAYHGGDVAAPIFREVAEQILPILG
                     VTPDIETKSAPDLVAEAHQDSERAAKLREEQAQAEQERKATLPTVDSNGGRGSEVVYA
                     VSRQKSMLMPDLRGRSVRDVARTC"
BASE COUNT          921 a         1129 c         1208 g          856 t
ORIGIN      
        1 taggcgtcgc ggcgtccact gtccttccta tcctgcctct cacaggcata caggaggcga
       61 acacttcaat ccgaacacag tattgacagt gctttgagcg cagcgtagta tccgaagtcg
      121 ggtggtcaaa agtggtcgga agtgggtcca acctcgtagc aacaccgatc attaagcaac
      181 acttttccta aggccacccg ccaagctctc tcgtcaggta gaaactatgc tgcgaggaaa
      241 ttacacggcg cgtatagatg tcaaaggtcg tctgaaaata ccgaccgagt tccggcgcct
      301 gatcgaagag aagcattgca aagattttta tatcacgagc ttgaccggcg attgcgtgcg
      361 gatttatccg ctgccggagt gggagtcgat cgaacaacga ctttcactgc tgccgtcgat
      421 ggatcccgcg cgccggaagt tccttgacag aacgaactac tacggtcagc aggcgacgat
      481 agatggccag ggccgcttgc tgattcaccc gttgcttcgt aagagcgcgg aagttattgg
      541 cgacgtcgcc gtgctcggct acctgacgta tttggaagtt tgggaactcg acaagttccg
      601 ggcccggcta cttgccgatc cttacacgga agaagacgaa gcggcgattg ctcgacttgg
      661 tatttagtgc tttgagcttt gtacttagtc cttggtgctt tgtaagaatg aaggagtgac
      721 caggaacaaa gaacaaagaa caaagaacaa gaaaaggatc tttgaacgag atcacggggc
      781 ggtggtgatg gctgcgcctt tggggggcat aggcgcgccc catcgccccg tgctgctgaa
      841 agagactttg cagttcctcg ttcttgaacg cggaggtctc ttcattgact gcacggttgg
      901 acttggcgga cacagcgaag cgattctgca agcctctgag cgtactcgtg tcatcgcaat
      961 ggacctcgac ccgtcggcgc tcgcgtactc acggaaacga ctcgcaacct acggtgaaag
     1021 gtttcgcgcg tatcaagcca actttcgcga actggcagac gtgttaagtc gcgcgggcga
     1081 acgcgacccg gacggaattc ttgtcgatct cggggtctct tcactgcagt tcgattcccc
     1141 tgagcgcggt tttagttttc gtttcgatgc tccgttggac atgcgcatga atccaacgat
     1201 cggagccacc gcagccgatc ttcttcagca acttcccgaa tcggaaattg ccagaatcat
     1261 ttttgaattc ggcgaagagc gacattcccg gcgtatagct cgtcgcctcg ttgaaagacg
     1321 tgaactgggt aatccggtaa ccaccacgac agagttagcc gatctcgtca tggcggctgt
     1381 cgggggcggt aaaaggcagc agattcatcc ggcgacgcgc accttccagg cgctgcgtat
     1441 cgccgtcaat aacgaactcg aagggttagg tgattttgtt gaattagcag tcgatctttt
     1501 agcacccgac ggccgctttg tgggaatcag tttccactcg ctcgaggatc ggattctcaa
     1561 acaggcgttg cgacggttgt cgggccactg ccaatgtccg ccacgtctgc cggtctgttc
     1621 gtgcggggcg agagaagcgg ttgaagtatt gacccgccgt ccagtagcgc ccagcgctcg
     1681 tgagattgaa gagaatccgc gagcgcgcag cgcgaaactc agagcagccc gcacagtcgt
     1741 ctcccggtga cgcgcgcgac cagaaacgga tcttcgtaac tacaaggggg gtaatagaac
     1801 atgaaacgat ttcccgagca ccagcttaac gcgaagattc gccgcacacg cgacgtgaca
     1861 gcgctctccc gtttggcgct gctgttgttc tgcggcctgg tactggcagg cggttttgtc
     1921 tttgccgcca agcaacactt cgctgccgtc caatacggtt atcagaacga acacctccgc
     1981 agcgagcgca agagattgtt ggaagaaaac cagcgacttg cgcttgataa ggaacgtgtg
     2041 acggcgcctg ataagctgga gcccgccgct cggcgcttgg gactaaagcc tgcgaggccc
     2101 ggccagattg tgactcttaa ccacagatga cacggacgaa ctcctttccg tgaagagcag
     2161 tgtaaatccg tggcggttat cgctaaatca aagtccagac ccgatgtctc gctcagccgc
     2221 gcgcttttcg tcgcggcttt tattggcttc tggatgctga ttgtttgtgc gaggttagtc
     2281 cacctgcaat tctcacaaca caacgggctc gccaatcgcg cacgactgca acaacagaac
     2341 gccattgaaa ccactccgca acgtggcgaa ctgctcgacc gtcacggccg gcagttagct
     2401 cgcagcgtgc aaacggtttc gttgtttctc gatcccgacg gcctcgacgt cggcgagctc
     2461 gaatgcactg cccagtatct ctccgtttcg ctcggcatga agtacaagaa tctgctggct
     2521 cagtttcgcg aaggtcaagc tgagaaacga agatttatct ggatcgcacg acggctcgac
     2581 gcggaccacg ccaacagaat cgtggcgatg aacttgcccg gtttgcagtc agtgctcgag
     2641 ccgaagcgtt actacccgaa tggaccactg gcagcacacg ttctcggtta cgttggactc
     2701 gatggagacg gtctcggcgg actcgaacgt ttctacaact caaagatctc tggagagccc
     2761 ggccggctct ttctcgaaag ggatgcgaac ggaaaaccgt acgagagtta tgagatcgcc
     2821 tcgaagccgg gccaaacagt cgtattaacg atcgaccaat cgatccaata ccaggcagag
     2881 caggcgctga acaacgcggt gaagagttcg cgtgcgaggt ccggaacggt gattgtgctg
     2941 gatccacgca gcggagacgt gctcgcgctt gcgaatgctc cgtccttcga tccgaacaac
     3001 gtgtcggctt cagcggcgga aacgcgttcg aactgggcgc tgcaaaacat ctacgaaccg
     3061 ggctcgacgt tcaatgtggt tgcgttctca gccgcgctgg agaagaaact cgcgagtgta
     3121 gacgaccaca tcgactgcca gatgggtgcg attacagtcg ccggacgcgt gattcacgat
     3181 cataaaccgt tcggtacttt gacgatctcc gaagcactcg cgaagtcgag caacgtcgct
     3241 gcgatcaaac tcggtctgcg cgttggcgac gaaacgatgt atgactacat cacgcgtttc
     3301 gggtttggat ctaaaacagg gattgagcta cctggcgaaa cggcgggact cttgcgcaag
     3361 gttgaaagat ggcagccttc gtcgatcggg tcaatcgcga tcggtcagga aatcggcgtc
     3421 acgcccgtgc agatggccgc ggcgtttggt gcgctcgcaa atgacggcgt gagggtcgcg
     3481 ccccacctca ttcgcgaagt tcgtaacgaa gcaggtgcgg tgatctatca accacaaccg
     3541 gagcagcgcc gtgtgatcaa cgctgaaaca gcgatcgcgc tgcgcggtat gctcgaaggc
     3601 gtgaccctgc gaggcacggc caaacaggcg cagttggatg gttactcagc cgcgggcaag
     3661 accggcacag cgcaaaagat cgatccgaag acgcgcgcgt attcagcgac aaagtttgtc
     3721 gcttctttcg ttggctttgc gccggtttca aatccgcaga tcgtgatcgt cgtcgtgatc
     3781 gacgaaccga gcggcgccta tcacggcggt gatgtggccg ctccgatctt tcgtgaagtg
     3841 gctgaacaga tcctgccgat tctcggcgtg acgccggaca ttgaaaccaa gtcagctcct
     3901 gacctggtgg ctgaggccca tcaggattcg gaacgtgcgg cgaagttgcg cgaggagcag
     3961 gcgcaggcgg aacaagagcg gaaagccaca ttgccgaccg tggatagcaa cggaggccgc
     4021 ggcagcgaag ttgtttacgc tgtatcccgg cagaagtcga tgctgatgcc tgatttgcgc
     4081 gggcgcagcg tgcgcgatgt cgcacgaacc tgcg
//
LOCUS       DASTEY010000561         5714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1496211, whole genome shotgun sequence.
ACCESSION   DASTEY010000561 DASTEY010000000
VERSION     DASTEY010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496211"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..517)
                     /locus_tag="VFO72_09840"
     CDS             complement(149..517)
                     /locus_tag="VFO72_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9479636.1"
                     /translation="MAATILIAEDYDDNRELLRLLLSGANYVVREARNGQECLTLAHI
                     DPPPDLIMVDLSMPELDGWEVFKQLKSNSNTANIPCVAVTAHTDRDRIRALQSGFSDF
                     VGKPFKTEELLLTVARLVQK"
     gene            745..2187
                     /locus_tag="VFO72_09845"
     CDS             745..2187
                     /locus_tag="VFO72_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479637.1"
                     /translation="MVNGHLEDSLRREIESYLENRLSAVKQEISTLQSLLNESLSNFV
                     ERQSDVQLESSLVASITEHLHAAHERGIEHAASESSRAKASSDMALVKSAISEINEQK
                     SQADILKALVNRASSFAPRVAFFVVKGDQATGWRGRGFEGTVGDQAIPQISLSLSSDT
                     VIGSAAKALTTWSGGPGSHAEDHLLLNRLGEEPPQRIVAIPLVVRGRSVAVLYADSAA
                     LDSESIALEALETLVTVSGMAVELLSVSRAAPKRAAVEEAAPAPTQEPSYVPSSEYEP
                     SSQTASEPSYQVTPEPAYEPTLEPVYEVAPEPAFSGADTISTYDAEPAERGSLMDSVE
                     EATTARDDATQTSGVLGEEPQAWSSTPVEEAPAQPRRRFGQDAELPVEVHGEEERRLH
                     NDARRFARLLISEIKLYNEQKVAEGRMEHDLYDRLREYIDRSREMYDKRVKAEVAARY
                     DYFHGELVNTLAEGDVSKLGSNYPGATVNA"
     gene            2264..4510
                     /locus_tag="VFO72_09850"
     CDS             2264..4510
                     /locus_tag="VFO72_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9479638.1"
                     /translation="MAKHRLLLISIAIALVGVASLPFLMSASCLTRQQLPAEQKALET
                     LRNMTRNDVLPSEAAVVAIENQFPRTKTAALARLVRARIKLNSGDFAGAATLLNTNLI
                     AEQTSIADHALFLRASALEQAGRTQEARAIYQQIMQQYPTSLRAREAALAAANLLTKT
                     GEPAAVPLLLKDLSETNDAAALLATAKAYEQTSDTTRALASYRRLYFFAPESAESADA
                     ATAIPRLGSTLSPANAEEAMARADRLYTAKRFNDALTAYTDAFIKFPATATNENRLRR
                     GIAAANARKTTDAVAALNSIPAAAGETRAEALFYLAQTYARARQWEQARATVEELRRV
                     FPASNFTPKAFVAAGQIAKEAKNDVDANYFLRAAVNGFSSSIEVAQAQFDLAWAAHEA
                     RNFAESSRMLTEHLAYYADKNTDNRGRAGYWAARDSERAGKLAEARALYEAMLGRYDA
                     NWYGYLAKQRFDVLSRTTPAKTFPPDSIVARAVANLQTVTVAEESAGPEAETRIIKAD
                     ELSNVGLDELALKELSKAGEDTPASPKVNLAIAKIYRSRDDNVRALNTLRRSFPDYSQ
                     MKPEEMTREQWDVFYPLAYWDIIVQESRARNLDPYQVAGLIRQETIFTARARSSAAAY
                     GLMQVLVPTARLTARKYGVSREISAESLYEPRLNVQLGTAYLRDQIDKFGRIEYVAAA
                     YNAGPGRAVQWKASLPAEIDEWAEAVPFKETRGYIQGVVRNRLQYLRLYDEKGNFRPE
                     VGSRANHR"
     gene            4507..5385
                     /locus_tag="VFO72_09855"
     CDS             4507..5385
                     /locus_tag="VFO72_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479639.1"
                     /translation="MIIRVYLCLLVFAVVVHAQPAALTITTEPNAIVWIDEVRRGTTD
                     ASGKLTLTKLSRGRHTVRVRAKGFKETTTALLPGRRTLAVKLVPTTDQAELLFQDAET
                     AREQARDNAAREKAADLYRQVLKARPAYPEANVGLARVLLDLNQFKEARAAIDAARRT
                     RPVYPEASAVEGRIYREEAFDEEAIRSFRRAIREARGFQPEAHVGLARVLEDKGDSAA
                     AVVELRKALDQLSDSEPVIYQMLGAVYEKLNKPKDAIVAYEKYLQLAPNGSYAAAIRS
                     ILDQLKREAAGEEIIP"
     gene            5458..>5714
                     /locus_tag="VFO72_09860"
     CDS             5458..>5714
                     /locus_tag="VFO72_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479640.1"
                     /translation="MATPSFSKHRHVSQRASSFTESVIREMTREAMKHGAVNLSQGFP
                     DFAAPEDIKRKAMEAIAADVNQYAITWGSKDFRDAIARKTRS"
BASE COUNT         1324 a         1656 c         1614 g         1120 t
ORIGIN      
        1 ggaaggcgtg gtctacaaaa taagagcgac tgtttactct gggaccgccg gcgtctcacc
       61 tgctttcttc gaacggggtc gctgccgtga gtttgcaggc gagacgcctg cggtcccagg
      121 acgaagacgt ccaggttaaa cgttatcgct atttctgaac taaccgggcc acagtcaaca
      181 gcagttcttc agtcttgaaa ggtttcccaa cgaagtcgct gaatcccgac tgcaaggcgc
      241 gtatgcggtc gcgatctgtg tgcgcggtta ccgccacgca cggaatgttg gctgtattgg
      301 aattagactt gagctgtttg aaaacctccc acccgtctaa ctccggcatg gaaaggtcga
      361 ccatgatcaa atcgggcggc gggtctatat gcgccaaagt caggcattcc tgcccgtttc
      421 tagcctcacg aaccacataa tttgcgcctg atagcagcaa acggagcagt tcgcgattgt
      481 cgtcgtagtc ctcagcaatc agaatcgtcg cagccatagg attagggggt tttcctccat
      541 tggtctaccg atcgtggcaa ccaagatacc agagccctcg tgcatcaaca aaaaccttat
      601 tgactagctc aataccgccc gtccataatc agttcgagcc cgtgccccct gccaggctct
      661 ctttgcgtaa agaccttggg ttagtttccc tacctgaaca ccttaggttc gtcacatcaa
      721 caccacgaca cggaggaccc tactgtggtc aacggacatt tggaagacag tttacggcgt
      781 gaaatcgaaa gttatctcga aaatcgtctt agcgccgtaa aacaggagat atccacgctg
      841 caaagtctgc tgaatgaatc actttccaac tttgtcgagc gccagagcga cgtgcagttg
      901 gaaagcagcc tggttgcgtc gataaccgag cacttgcacg ccgcgcacga acgcggtatc
      961 gaacacgccg cgtcggagtc gtcgcgagca aaagcttcca gtgacatggc cctggtgaag
     1021 tcggcaattt ccgagattaa cgagcagaaa tcgcaggcag acatcctgaa ggcacttgta
     1081 aaccgggcct cctcgtttgc tccgcgtgtt gccttcttcg tcgtgaaagg tgaccaggca
     1141 accggctggc gtggacgtgg ttttgaaggc accgtcggcg atcaggccat tccccagatt
     1201 tcgctgtcgt tgagctccga cacggtaatc ggcagcgccg caaaagcgct tacgacgtgg
     1261 agtggtggac caggttcgca tgccgaagat cacctgttgc tcaaccgcct cggcgaagaa
     1321 cctccgcaac gtatcgtcgc aattcccctg gttgtgcgtg gacgctcggt agcagtgctc
     1381 tacgccgact cagccgcgct agattccgaa tcgatcgctc ttgaggcact cgaaacgctt
     1441 gtcacagttt cgggcatggc ggtcgagctt ctgtccgtat cgcgtgccgc cccgaaacgt
     1501 gcggctgtcg aagaagcggc gccggcgcca acacaggagc cttcgtacgt tccgtctagt
     1561 gaatacgaac cgtcctcgca aactgcatca gagccgtcgt accaggttac gcctgaaccg
     1621 gcatacgaac cgacacttga accggtttac gaagttgcac ccgaaccggc attctctggc
     1681 gctgacacga tctcgaccta cgatgccgaa cctgctgaac gcgggtcgtt aatggacagt
     1741 gttgaagaag caaccacggc gcgcgacgat gcaacacaaa cgtcaggcgt tctgggtgaa
     1801 gagccgcagg catggagctc gacgccggtt gaagaggccc cggcgcaacc gcgaagacgt
     1861 ttcggtcagg atgccgaact gccggtggaa gttcacggcg aggaagagcg ccgcttgcac
     1921 aatgatgctc gccgctttgc acgactgctg atctctgaga tcaagttgta taacgagcag
     1981 aaggttgctg aagggcgaat ggaacacgat ctttacgatc gtttgcgcga gtacatcgat
     2041 cgttcacgcg agatgtatga taagcgcgtg aaggcggaag tcgcggctcg ctacgactac
     2101 ttccacggag aactcgtgaa cacgctggcc gaaggcgacg tttcaaagct gggatcgaat
     2161 tacccgggcg cgacagtgaa cgcttaggtt ggttcttggc actttgtgct tggtagttcg
     2221 tggcgcgctt tacaaagaac aaaaatctaa gcacaaagaa cagatggcta agcatcgctt
     2281 actactgatt tccatcgcga ttgcacttgt cggcgttgca tcgctaccgt ttctcatgag
     2341 cgctagctgc ctcacgcggc agcagcttcc agcagaacag aaggccctcg aaaccctgcg
     2401 caacatgacg cgcaacgacg ttttgccgtc tgaagctgcc gtggtggcga tcgagaacca
     2461 gtttccacgc acgaagaccg ccgcactcgc tcgcctcgtt cgtgctcgca ttaaacttaa
     2521 ctccggagac tttgccggcg ccgcgacgtt gctcaacaca aatttgatcg ccgagcagac
     2581 gtcgattgcg gaccacgcac ttttcctacg cgcgagtgcg ctcgaacagg caggccgaac
     2641 gcaggaagcg cgcgcaatct accagcagat aatgcagcaa tatccaacgt cgctacgtgc
     2701 gcgcgaagcc gcgttggcgg ccgcaaacct tttgaccaaa accggcgagc cagcggccgt
     2761 tccactgctg ttgaaagatc taagtgagac aaacgacgca gccgcgttgc tggccactgc
     2821 aaaagcttac gagcagactt ccgatacgac gcgcgccctg gcttcgtatc gccgtctcta
     2881 tttctttgcg ccggagtccg ctgagagtgc cgacgcggca acggcgattc cgcggctcgg
     2941 ttcaacgttg tcgccggcca atgctgaaga ggcaatggca cgcgcggatc gtttgtatac
     3001 ggcgaaaagg ttcaacgacg cgctcaccgc ttacactgac gcgttcataa agtttcccgc
     3061 gacggctaca aatgaaaacc ggcttcgacg tggaatcgca gccgcgaatg cacgcaaaac
     3121 caccgatgcc gtcgcagcgc tgaacagcat tccggctgcc gcgggtgaaa cacgcgccga
     3181 ggcgctgttc tatctcgcgc agacatacgc gcgtgcgcgg cagtgggaac aggcacgcgc
     3241 cacggtcgaa gagttgcgcc gcgtttttcc ggcgagcaat ttcactccca aagctttcgt
     3301 ggccgccggt cagatcgcaa aagaggcgaa gaacgacgtt gacgcgaatt actttttgcg
     3361 cgcggcagtg aatggttttt ccagttcgat agaggtggcc caggcgcagt ttgatctcgc
     3421 gtgggccgcg cacgaagcac gtaacttcgc tgaatcgtcg cgcatgctta ccgagcacct
     3481 cgcttattac gccgacaaga acacagataa tcgcgggcgc gccggttact gggccgcgcg
     3541 cgactccgaa cgcgcaggca aacttgcgga agcgcgagcg ctctacgaag ccatgctcgg
     3601 ccgctacgac gcaaactggt atggatatct tgctaaacag cgcttcgatg ttttgagccg
     3661 gaccacgccc gcgaaaactt ttccaccgga ttccatcgtt gcgcgtgcgg ttgcgaacct
     3721 ccaaaccgtg accgtcgcgg aagaaagcgc cggaccggaa gctgaaacac gaattataaa
     3781 agcggacgaa ctgtccaacg tcgggcttga cgaactcgcg ttgaaggaat tgtccaaagc
     3841 aggcgaggac actcctgcca gtccgaaagt aaacctcgcc atcgcgaaga tctaccgctc
     3901 aagagacgac aacgtacgcg cgctgaacac gctgcgccgc agttttcccg actactcgca
     3961 gatgaaaccc gaagagatga cgcgtgaaca gtgggacgtg ttttacccgc tcgcctactg
     4021 ggacatcatt gtgcaggagt ctcgcgcgcg aaatctcgat ccttatcagg tggccggtct
     4081 gattcgtcag gagacgatct tcacggcgcg agcgcgatct tcggcagcag cgtatggact
     4141 gatgcaggta ctcgtgccga ctgcgcgcct gaccgcgcga aagtatggcg tgagccgcga
     4201 gataagcgcc gaaagcttgt acgagccacg gttgaatgtt caactcggca ctgcttacct
     4261 gcgcgaccag attgataagt tcggacgtat cgaatatgtc gccgcggctt acaacgcggg
     4321 accggggcga gcggtgcagt ggaaagcatc gttgccggcg gaaattgatg aatgggccga
     4381 ggcggtgccg tttaaggaga cgcgcggata cattcagggc gtcgtgagaa accggctaca
     4441 gtatctgcgg ctctacgatg aaaagggaaa cttccgcccg gaagtcggat cgcgcgctaa
     4501 ccacagatga ttatccgtgt atatctgtgt ctacttgtgt tcgcagtagt ggtccacgcg
     4561 caaccagccg cgctgacaat cactaccgaa ccaaatgcga tcgtctggat cgacgaagta
     4621 agacgcggca caactgacgc ttcaggcaaa ctcacactga caaaactctc gcgcggccgc
     4681 cacaccgtac gcgtacgcgc taaaggtttc aaagaaacaa ctaccgcact gctgcccggc
     4741 cgccgcactc tggctgtgaa gctcgtgccc accacagacc aagcggagtt gcttttccag
     4801 gacgcagaaa cagcgcgcga acaagcgcgc gacaatgccg cgcgtgaaaa ggccgccgat
     4861 ctttatcgac aggtactcaa agctcgcccc gcgtatccgg aggcaaacgt gggactcgcg
     4921 cgcgtgctac tcgacctcaa ccagtttaaa gaagcgcgcg ccgcaatcga tgctgcgcgt
     4981 cggacgcgac ctgtctatcc ggaggcttcg gcagtcgaag ggcgcatcta tcgagaagag
     5041 gcattcgacg aggaggcgat tcgttcgttc cgccgtgcga tccgtgaagc gcgcggcttc
     5101 caacctgaag cgcacgtcgg tctcgcgcgt gtgttggaag acaaaggaga ttccgcggct
     5161 gcggtggtcg agttgcgcaa ggcgttggac cagctctccg acagcgaacc cgttatctac
     5221 cagatgctgg gcgcggtata cgaaaagctt aacaagccga aagacgcgat cgtcgcttac
     5281 gaaaaatacc tgcaactcgc accaaacggc agttacgccg cggccatccg ttccattctt
     5341 gaccagctaa aacgcgaagc cgccggcgaa gagatcattc cttgacacag ataaacacag
     5401 atggaatttg gattttaccc gtgtaatttg tgtttatctg tggcctaata acaaggcatg
     5461 gcgacaccct cattttcaaa gcaccggcac gtctcccaaa gggcgagttc gtttaccgaa
     5521 tcggtgattc gggagatgac tcgtgaggcg atgaagcacg gcgcggtcaa cctctcgcaa
     5581 ggcttccccg attttgccgc acccgaagac atcaagcgca aagccatgga ggcgatcgcg
     5641 gccgacgtca accagtatgc cattacgtgg ggctcaaaag actttcgcga cgcgatcgcc
     5701 cgcaagaccc gttc
//
LOCUS       DASTEY010000562         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1430313, whole genome shotgun sequence.
ACCESSION   DASTEY010000562 DASTEY010000000
VERSION     DASTEY010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430313"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1221
                     /gene="murG"
                     /locus_tag="VFO72_09865"
     CDS             136..1221
                     /gene="murG"
                     /locus_tag="VFO72_09865"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9479641.1"
                     /translation="MRVLIAAGGTGGHIYPGIAVAQEIMRRNPESRVRFVGTARGLET
                     RLVPQAGFELTLIDSAGLKNVSLVARMRGLAILPKSFVSARRVIREFQPDVVVGAGGY
                     VSGPVVLMASFLRRPTLVMESNALPGWTNRVLARFVDRAAVSFEQALPYFRGKAVVTG
                     NPVRREFFEIPPKRREPGRLSLLVFGGSQGARAINEAMVAALPGLKNLPVTLRIKHQT
                     GAADFEKVKAGYNAAGWNEQTEVCSYIDDMMSDFAQADLVICRAGATTTAELIAAGKA
                     SIMIPFPFAADDHQRKNAEALQVAGAARMIVQQELSGKRLFEEIRDLARDPLKIDRME
                     EASRGLAHGDAAKAVVDLIESLVGTGM"
     gene            1225..2619
                     /gene="murC"
                     /locus_tag="VFO72_09870"
     CDS             1225..2619
                     /gene="murC"
                     /locus_tag="VFO72_09870"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET9479642.1"
                     /translation="MFRRIQHVHFVGIGGIGMSGIAEVLINLGFRVSGSDLKRSSVTD
                     RLQQMGVEIYEGHSADNVGHPHVVVRSTAVRDDNPEIVQANQRSIPVIPRAEMLAELM
                     RLKPHTVAVAGSHGKTTTTSMVATVLGHAGLDPTMVVGGVVGAYGSNAHLGTSDLMVV
                     EADESDRSFLMLTPTIAVVTNIDREHMDYYHDMDDVRECFANFVNKVPFYGSSVLCLD
                     DPNVQAIIPLLERRRMTYGLSAQADVSAHGIRHDNNFGSSFSVWRGTDVVGEMMLRVP
                     GVHNVYNALAAIAVGFELEVPFEKIAEGLHSFTGAGRRFQAKGEVNGVLVVDDYGHHP
                     TEIRATLAAAKLGSGGRRIVVLFQPHRYTRTHDLMQEFARSFNNADVLFITDIYAANE
                     DPIEGVTAEALTAAIKRFGHKDVNYIGPLENAAPALSECVQPGDLVLTLGAGTVNRVS
                     EQLLFLLREQSQGK"
     gene            2657..>3121
                     /locus_tag="VFO72_09875"
     CDS             2657..>3121
                     /locus_tag="VFO72_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9479643.1"
                     /translation="MEQRDHALEEIARRTGVRAERGRRFAELTSLRVGGSIDWVISPD
                     TEEQAAAIVYELDQAGIGWRPLGSGSNVLADDGDHHYVVLSLSDMKGEVRIDGELVSV
                     SAGYSLPRLCIDVARAGLSGIEGLGGIPGTVGGALWMNAGAYGHEIGTVTETV"
BASE COUNT          643 a          783 c         1013 g          682 t
ORIGIN      
        1 ggtcggcatt ctgcttaacg tgtcgcagca cgcgagcggc tcgctcgacg ttggactctt
       61 tgaaactgcg acaactacgc cgcgcgagcg caagcaatta gacgtcgcgc cggcgagtcg
      121 gttctgatca ttgacatgcg agttttgatc gcggccggtg gcactggcgg acacatttat
      181 ccgggaatcg ctgtcgcaca ggaaatcatg cggcgcaatc ccgaatcgag agttcgtttt
      241 gtaggcactg cgcgtggtct ggaaacacga ttggtcccgc aggcgggatt cgagttgacg
      301 ctcattgaca gtgcgggttt gaaaaacgtc agtctcgttg cgcgcatgag gggtttggcg
      361 atcctgccga agagttttgt ttcggcgcgg cgcgtcattc gggagtttca gcctgacgtg
      421 gttgttggcg caggtggtta tgtttccgga ccggtggtgc tgatggcgtc gtttttgcgg
      481 cgtcccacac tggtgatgga gtcaaatgcg ttgccgggtt ggaccaaccg cgtgctggcg
      541 cggtttgtgg atcgtgcggc ggtctcgttc gaacaggcgc tgccttactt tcgcggcaag
      601 gcagttgtga cgggcaatcc ggtgcggcgc gagttttttg aaataccgcc gaagcgtcgc
      661 gagccgggcc ggttgtcact gttggtcttc ggtggctcgc aaggtgcgcg cgcaattaac
      721 gaggctatgg tggcggcgtt gccagggttg aaaaatctac ccgtgacact gcgcatcaaa
      781 caccagacgg gcgcggcgga ttttgaaaaa gtgaaggctg gatacaacgc cgcaggttgg
      841 aacgaacaga cggaagtatg cagctacatt gacgacatga tgagcgactt cgcacaagcg
      901 gacctggtga tttgtcgcgc cggcgcgacg actacggcgg agttgatcgc tgccggcaag
      961 gcgtcgatta tgattccgtt tccgttcgcg gcagacgatc atcagcgtaa aaacgcggag
     1021 gcgttacagg ttgccggagc ggcgcgaatg attgtgcagc aggagttgag cggcaagcgt
     1081 ttattcgagg agatccgtga tttagcccgg gatccgctca agatagatcg tatggaagag
     1141 gcgagtagag ggttagcgca tggagacgcc gccaaagccg tggtggacct gattgagagt
     1201 ctggttggga cagggatgtg agggatgttt agaaggatac agcacgtcca tttcgtcggc
     1261 atcggtggta tcggcatgtc cggcatcgcc gaggtgctga tcaatctcgg ctttcgtgtt
     1321 tccggttccg acttgaaacg ttcgagcgtg actgatcgct tgcagcaaat gggtgttgaa
     1381 atttacgagg gtcattcggc agacaacgtc ggccatccgc acgtcgtggt gcgttctacc
     1441 gctgtacgcg acgacaatcc cgagattgtt caggcaaacc agcgttcgat ccccgtcatc
     1501 ccgcgcgcgg agatgctcgc ggagttgatg cgactcaagc cgcacacagt tgcggttgcc
     1561 ggttcccacg gtaaaacaac gacgacttcg atggtcgcga cggtgctggg ccatgccgga
     1621 ctcgatccga cgatggttgt cggaggcgtg gttggcgctt acggttcaaa tgcgcacctc
     1681 ggcactagcg atctcatggt cgtcgaagca gacgaaagcg atcgatcgtt tctgatgttg
     1741 acaccaacga tcgcagtggt aacaaacatc gatcgcgagc acatggacta ctaccacgac
     1801 atggacgacg tgcgggagtg cttcgcaaat tttgtgaaca aggtgccgtt ctacggctcg
     1861 tctgtgttgt gtctcgacga tccaaacgtg caggcgatca ttccactgct ggaacggcga
     1921 cgcatgacct acggcttgag tgcacaggct gacgtttccg cgcatggaat tcgtcacgac
     1981 aacaatttcg gttcgagttt tagtgtgtgg cgcggtacag acgtagtcgg cgagatgatg
     2041 ttgcgtgtac cgggcgtgca caacgtctac aacgcgttag cggcaatcgc cgtgggattt
     2101 gagttggaag tgccgtttga aaagattgcc gaaggacttc acagctttac cggcgcggga
     2161 cggcgttttc aagcgaaagg cgaagtcaac ggcgttctcg ttgtcgatga ttacgggcac
     2221 catccgactg agattcgcgc gactcttgcc gccgcgaaac tcggttcggg aggccgccgg
     2281 atcgtcgttc tatttcaacc gcatcgctac acgcgcacgc acgatctgat gcaggagttt
     2341 gcgcgttcgt tcaacaacgc cgacgtactg ttcattactg acatttacgc cgccaacgaa
     2401 gatccgattg aaggcgtaac agccgaagcg ctgactgcag caatcaagcg tttcggtcac
     2461 aaagacgtca attacattgg accactcgag aacgcggcgc cggcgttgag cgaatgcgtt
     2521 cagccgggag acctggtctt aacgctcggc gcgggaaccg tgaaccgggt cagtgagcag
     2581 ctactgtttt tgttgaggga acaatcacaa ggcaagtaga aggtcgaagc cagatttgcg
     2641 aatagaagaa aaccaattgg agcaacgcga ccacgcactc gaagagattg cgcgaaggac
     2701 aggtgtgcgt gccgagcgcg gtcgccggtt tgccgagttg accagtttgc gtgtcggcgg
     2761 gtcgattgat tgggtgatct cgcctgacac tgaagagcaa gcggccgcga tcgtttacga
     2821 gttggaccag gcgggcatcg gttggcgtcc gctcggctcg ggcagcaacg tgctcgccga
     2881 cgacggcgat catcactacg ttgtgttgag tttgagtgac atgaaaggcg aagttcggat
     2941 cgacggtgaa ctggtttcag tgagcgccgg ttattcgctg ccgcgccttt gtatcgacgt
     3001 tgctcgcgcg ggactttcgg gaatcgaggg gcttggcggc attcccggaa cagttggcgg
     3061 cgcgctttgg atgaacgccg gagcgtacgg tcacgagatc ggaaccgtca ccgaaacggt
     3121 a
//
LOCUS       DASTEY010000563         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1545160, whole genome shotgun sequence.
ACCESSION   DASTEY010000563 DASTEY010000000
VERSION     DASTEY010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545160"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VFO72_09880"
     CDS             complement(<1..1242)
                     /locus_tag="VFO72_09880"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479644.1"
                     /translation="MAIDRISVKGARQHNLKNINVEIPRDRFTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDQLEKPDVDSVDGLSPAISIEQKTVSRSPRSTVGT
                     VTEIYDYMRLLFSSIGIPHCHVCGAAITRQSVEQIVQNVLGLPEGERVMVLAPIVRGR
                     KGEFKKELEKLAKEGFLRARVDGDLLSLDEEIRLDKRRNHTIEAVVDRLLIKPGIRDR
                     LTDSIRTALKLTSGAVLISIVDGEEKLYSERMACVNCGINVPPLEPRSFSFNSAYGAC
                     KRCHGLGTVLEIDPAKLIPDANLPVDKLAFMGPADKQGSAYLKSALQAVIKHCGANPK
                     TIFAELPKEVHDAFFNGASGKLSFSQGLYSYQSEWKGALNWLRDRLNEAPSERIRVAL
                     EELVSPTVCQQCNGRRLRSDSL"
     gene            1381..2115
                     /gene="pgsA"
                     /locus_tag="VFO72_09885"
     CDS             1381..2115
                     /gene="pgsA"
                     /locus_tag="VFO72_09885"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9479645.1"
                     /translation="MNLPNALTLLRIFIVPLLVVVLLTPFSEDWFGVPRHVLGVLMFL
                     GAALTDYLDGHIARSRGQVTRLGQLLDPIADKLLISAALISLVENQLAPAWAVVIIIG
                     REFAVTGLRSIAAVDGVVISASKMGKFKMLAQVITVALLIGSSVAGKPPVANFGRPFP
                     AIEFWSVPELRTAWQHLISEGAITGNDWQVLIYTAGRAMLWLVVISACASMYGYFSSF
                     YRTVIASRAAEPKTSHGLTQINTDHF"
BASE COUNT          461 a          651 c          661 g          548 t
ORIGIN      
        1 caacgaatca gaacgcaaac gccggccgtt acactgctgg caaacagttg gcgaaaccag
       61 ttcctcgagc gcgactcgga tccgctccga cggcgcttcg ttcagacggt cgcgcagcca
      121 gttgagcgcg cctttccact cgctttgata ggaataaagt ccttgactaa aactcaactt
      181 gccgctcgcg ccgttaaaaa aagcgtcgtg cacctctttg ggcaattcgg cgaagatcgt
      241 cttcgggttc gcgccgcagt gtttgataac cgcctgcaac gccgacttga gatacgccga
      301 accttgtttg tccgcagggc ccatgaaagc gagtttgtcg accggcagat tcgcatcggg
      361 gatcagtttt gccggatcga tctccagcac cgtccccagt ccgtgacaac gcttgcacgc
      421 accataagcc gaattgaacg agaaggaacg gggttcgagt gggggaacgt tgatgccgca
      481 gttcacacaa gccattcgtt ccgaatagag tttttcttcg ccgtcgacga tcgagatgag
      541 cacggcaccg ctggtcagct tcaaagccgt ccggatcgaa tcggttaaac ggtcgcggat
      601 cccgggcttg atcaggagcc ggtcgacaac tgcttcgatc gtgtgattgc gacgcttatc
      661 gagtcttatc tcttcatcga gcgacagcag gtcgccgtcg actcgcgcgc gcagaaaacc
      721 ttccttcgcc agcttttcga gttccttttt gaactcgccc ttacgcccgc gcacgatcgg
      781 cgccagcacc atcacgcgtt cgccctcggg taatccgagg acgttttgta cgatctgttc
      841 gacactttgt cgcgtaatcg ctgcgccgca gacgtgacag tgcggaatgc cgatggacga
      901 aaacagcagg cgcatgtagt cgtagatctc ggtcacggtt cccactgtcg agcgcggact
      961 gcgtgaaacc gttttctgtt cgatggaaat cgcaggcgac aggccatcga ctgagtcgac
     1021 gtcaggcttt tcgagttgat cgagaaactg gcgggcatag gctgagagtg attcgacata
     1081 gcggcgctgg ccctccgcgt agatcgtgtc gaatgcgagt gaggatttgc cggaaccaga
     1141 caggccggta atcacagtga agcggtcccg cgggatctcg acattgatgt tcttaaggtt
     1201 gtgctggcga gcgcccttaa cactgatgcg atcaatggcc atagcgaaaa ccagccattc
     1261 tagcggacgg attttcgctc agcaagctgg ccacagacgc gtagcgccct ggtttggatt
     1321 ctctatccgc agattacaca gattgaccta gtttaaatag cgacctacac tgccagcacc
     1381 gtgaatcttc ctaacgcctt aacgctgctg cggattttta tagtgccgct gctggtcgtt
     1441 gtgttgctga cgccattttc tgaggactgg tttggcgtgc ctcgtcacgt tctgggcgtg
     1501 ttgatgtttt tgggcgccgc gttgactgac tatctcgacg gtcacattgc gcgcagccgc
     1561 ggacaagtaa ctcgtctggg ccaactgctc gatccaattg ccgacaaact tttgatctcc
     1621 gccgcgttga tttcgctcgt cgagaatcag ctcgcgcccg cgtgggcggt ggtgatcatc
     1681 atcggtcgcg aatttgctgt gactggtttg cgctcgattg ccgcggttga tggcgtggtg
     1741 atctcggcgt cgaagatggg aaagttcaag atgctggccc aggtgatcac tgttgcgttg
     1801 ctgatcggat ccagcgtggc tgggaaacct ccggtggcaa acttcggccg tccgttccca
     1861 gcgatagagt tctggtccgt gccggagtta cgcacggcat ggcaacacct gatcagcgag
     1921 ggcgcgatca ccggcaacga ttggcaggtc cttatttata cagcgggcag ggcgatgctg
     1981 tggctggtcg tgatctccgc gtgtgcttca atgtatgggt acttcagctc cttctatcgc
     2041 acggtcatcg ccagccgcgc cgcagaaccg aaaactagcc acggacttac acaaataaac
     2101 acagaccatt tctgacttgt gcttattcgt gcgagttccg tggctggtgc cttattggat
     2161 ctcgacccag tcgccggtgt gaacctcacc gaccgttctt gtgataatcg cagtcgcagt
     2221 tctcgtctgc acgtcgatga tcaccatctc acccacgatc ttgcgcggca atggcgggcg
     2281 gttgtttctg acttcgcgcg tcgtgatcgg tcgataacga t
//
LOCUS       DASTEY010000564         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1430356, whole genome shotgun sequence.
ACCESSION   DASTEY010000564 DASTEY010000000
VERSION     DASTEY010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430356"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..796)
                     /locus_tag="VFO72_09890"
     CDS             complement(56..796)
                     /locus_tag="VFO72_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479646.1"
                     /translation="MRYVVVLVLGVLLGAGVAIYFLGVAPAKSTPGVAVRPPDGQAAP
                     ATVVIGLEQSFVDAVLATTFSGLQNPTFQLSQSAGAGEFTTAAFQGGCANSITLLPEG
                     SGVKTGVQFRNGNVSAPLAFTGSYNFSGQCIQFKGWAQTNIKLYFEEPNQTVYGRVDV
                     EGVNLEGVNPIANTFVTVFVQTAIDQRVNPLVLVSEKQLSLLIPVKASNGTVRARAKD
                     VRAEILDGSLKLHLTYEFSGVRGEATAG"
     gene            complement(811..1641)
                     /locus_tag="VFO72_09895"
     CDS             complement(811..1641)
                     /locus_tag="VFO72_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479647.1"
                     /translation="MAETNVNMNVNTNANANMAPVNANSATAPSTLAAREPDRYRATL
                     VLSAETEGGEKTIGIPTLSAEVAKNGPDRRLSFKLPDGSDLIYIERNGVHYAVAPARK
                     QYAELTPEATGLQLHKLMTPGQLVAYLDRLQGIQLVGEETMAGRTAVKYRYARHDETK
                     TSAGEVRTESFVYIDKETGLPLRAELFSEATGNVQGARGATVVAEMRDISTEVDLSMF
                     EIPAGMNKVSEQQVRQQINAITNTLAAVLRTLLTNMNTAPPPSPSPTATVSPAASPTP
                     "
     gene            2205..2768
                     /gene="efp"
                     /locus_tag="VFO72_09900"
     CDS             2205..2768
                     /gene="efp"
                     /locus_tag="VFO72_09900"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9479648.1"
                     /translation="MAIPATQIRRGMVIVFEGQPCKVVDFRHHTPGNLRAMVQTKLRN
                     IRTGSSFEHRFRSADTVDRATLEQHEMEYLYSDGSHHHFMNTENYEQIALSEEDLGDA
                     VPWLTPGLKLQAEFYEGAPIGIDLPPSMELKVVQTEPTLKSATVSNVNKPATLENGVT
                     ISVPPFINEGDRIRVDPAEGRYIERAK"
     gene            2769..4109
                     /locus_tag="VFO72_09905"
     CDS             2769..4109
                     /locus_tag="VFO72_09905"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HET9479649.1"
                     /translation="MYFAYSLLLSLGLLLLSPYFLFQALAHRKYITGLRERLGFLPTI
                     NDQPVIWLHCVSVGETQAARPLVERIKKELPHHALVVSTVTLTGQKLARDLFRTQAVK
                     VFYFPFDWRWSVRRALNAVSPAAVLLMETELWPNFLRECKKREIPAAVVNGRISRRSF
                     GRYMKARFFLRRVLACLSMAVMQSERDAERIRALGLSAESVFTAGNLKFESGIYTDKH
                     TDVKERFGLRAEVPLVLAASTHAPEEKIVLESFKQLRDSRPVRLILAPRRPERFNEVA
                     ELIDESGLSWARRTNAPHARDVEAEVILLDTIGELPAIYPLATAVFVGGSIVDRGGHN
                     VLEPAAHGVAVITGAHTHNFNAIVDLLNNADAIVQLPPVEGAEAVTRLTEVLQRMLAD
                     ADWRIEIGTRAKELIIANQGAAERTMKLLAPLFSGKLSDKTPSDSLLPLNAHTS"
     gene            4106..>4867
                     /gene="lpxK"
                     /locus_tag="VFO72_09910"
     CDS             4106..>4867
                     /gene="lpxK"
                     /locus_tag="VFO72_09910"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET9479650.1"
                     /translation="MKSLILPPLSALYDVVTRTRLSLYRRGAFRTTRLDRPVISVGNI
                     TTGGTGKTPLVEWIARNLSNAGRKVCVLTRGYGRENPQSQVVVSDGQTILATPAEAGD
                     EPYLLATNLLGSAAVICNADRISAGRYAIEAFGTECFVLDDGFQHLRLARDLEIVTID
                     ATNPWGSGESHRNGGHLLPYGRLREPLSGLRRADCVVLTRCDQTDPANLEALKHQIRS
                     LIGGRPLFESTMRASRKPDAPGPVAAFCAVGNPESF"
BASE COUNT         1027 a         1391 c         1377 g         1072 t
ORIGIN      
        1 tcttcgagcg gcaacgacta ctcgcgcagg cagcagtgcc aacggacaat acggatcagc
       61 cggccgttgc ttcgccgcgc acaccgctaa actcgtaagt cagatgcagc ttcaacgagc
      121 cgtcgagaat ctcggcgcgc acgtccttcg ctcgagctct taccgtgccg ttgcttgcct
      181 ttaccggaat caacagcgac aactgtttct cactcactaa cacgagcgga ttcacgcgct
      241 gatcgatcgc agtctgcaca aacacggtga cgaacgtatt ggcgatcggg ttcacgccct
      301 caaggtttac accttcaacg tccacgcgac catagaccgt ctggttgggc tcctcaaaat
      361 agagtttgat attcgtctgg gcccagcctt tgaactgaat acactgcccg ctgaaattgt
      421 agctgccggt gaacgcgagc ggcgccgaga cgttgccatt gcgaaactgc acgcccgtct
      481 tgacgccgct tccttcgggc aagagagtga tgctgttggc gcatccaccc tgaaacgctg
      541 cggttgtaaa ctcgccggct cccgcagact gagacaattg aaaagtggga ttttgcagac
      601 ctgagaacgt tgtcgccagc acggcatcaa caaaactctg ttcgagccca atcacgactg
      661 tggcgggcgc ggcttgcccg tccggtggcc gcaccgcgac gcctggcgtt gacttggccg
      721 gcgccacgcc aaggaaatag atcgcgactc cagcgccgag caaaactcca agaacgagaa
      781 cgacaacgta cctcattgat caaactccct tcacggcgtt ggactcgctg ccggtgaaac
      841 agttgctgtt ggtgacggcg atggcggcgg tgctgtgttc atgtttgtga ggagcgtccg
      901 gagcactgcc gcaagcgtgt ttgtgatcgc gttgatctgt tgccgcactt gctgttccga
      961 gactttattc attccggccg gaatttcaaa catactcaga tcgacttccg tcgagatgtc
     1021 gcgcatctcg gcaacaaccg ttgcgcctct cgcgccctga acgtttccgg tcgcttcgct
     1081 aaaaagttcg gctcgcaatg ggagtcccgt ttctttgtcg atgtagacga aagattccgt
     1141 tcttacctcg cctgcggagg tcttcgtttc gtcgtgtcgt gcgtagcgat acttaaccgc
     1201 cgtgcggccc gccatagttt cctcgccgac tagctgaatg ccctgaagcc gatccagata
     1261 agcaaccagt tgtcctggcg tcatcaactt gtgtaactga aggcctgtag cctcgggggt
     1321 caactccgca tactgcttcc ttgccggcgc gacagcgtaa tgcacaccat tgcgttcgat
     1381 atagatcaga tctgatccgt caggtagttt gaacgaaagc cgccgatcgg gaccgttctt
     1441 ggccacttcg gccgagagcg ttggaatacc gatcgttttc tccccacctt cagtctcggc
     1501 actaaggacg agggtggcac gataccggtc aggttcgcgt gccgcgagcg tggaaggagc
     1561 agtcgctgag ttggcgttta cgggcgccat gttagcgttg gcgttggtat tgacgttcat
     1621 attgacgttt gtttcggcca caggcccgcg agaacatccg ctcgctgtta caagcatcgc
     1681 cgctgaaagc aggacgagaa aaagaaccgg tcgttgcatg acaggcagga atgcctgtcc
     1741 tacgtcggca gttttcataa ttggacaccc ctcgaaggaa ttcgcgctca ggcgtaacgt
     1801 tgataaccag ggggttttga gggaaggacc atagaatagc acggtcgcga cggtagtcag
     1861 aagagatgat tgacttcggt actgtcctaa tctgaacgcc agaggcgttc gctaattcca
     1921 gcccagggtt gcttcaaccc tgggtcgaac gttagagtac ggtcctaacg ctgaaggcgt
     1981 tggcaaattc gcagttagcc aacgctttca gcgcaaaagg taaaaacatc caatcaatcc
     2041 ccagggttgt cgcactccaa cctctgggct ggaattcgat aacgccttcg gcgttcagat
     2101 taggacacta cctggaaaat gcttcactgg acaaagctac aaaggcctcg ttagactacg
     2161 cgttttgtta aaacctctat tttttcgacg gagaattcag ttatatggct attcctgcaa
     2221 cccagatacg gcgcggcatg gtgatcgtct tcgaaggcca accctgcaag gtcgttgact
     2281 tccggcacca cacgcccggc aacctgcgag cgatggtgca aaccaagctg cgtaatattc
     2341 gtactggctc ttcttttgaa catcgcttcc gctccgcgga cacggtcgat cgcgccactc
     2401 tcgaacagca cgagatggag tatctctact ccgatgggtc gcaccatcac ttcatgaata
     2461 ctgaaaacta cgaacagatt gcgttgagcg aggaggatct cggcgacgcg gtgccctggt
     2521 tgacgcccgg cttgaagctc caggccgagt tttacgaagg cgcgccgatt ggaattgacc
     2581 tgccgccctc gatggaactg aaagttgtac agaccgaacc gacgctgaag agcgctacgg
     2641 tctccaacgt caataagccc gccactctcg aaaatggcgt gacgatttca gtgccaccgt
     2701 tcattaacga aggagaccgc atccgagtcg atccggcgga agggcgttac atcgagcggg
     2761 cgaaataaat gtatttcgcc tacagcttac tcttatcgct tggtctgctg ctgttatcac
     2821 cttacttcct gtttcaggcg ctcgcacacc gcaagtacat caccggccta cgcgagcgcc
     2881 ttggttttct tcccacaatc aacgatcaac cggtcatttg gcttcattgt gtctcggtag
     2941 gtgagaccca ggcggcacgg ccgctcgtgg agcgtattaa gaaggagctg ccgcaccatg
     3001 cgctggttgt ttcgactgtt acgttgacgg gacagaagtt ggcgcgcgat ctgtttcgca
     3061 cgcaggctgt gaaggtcttt tattttccgt ttgactggcg gtggtctgtg cgccgcgcgc
     3121 tcaacgcggt gagtcctgcc gcagtgctat tgatggagac cgagctctgg ccgaattttc
     3181 tgcgcgagtg caagaagcgc gagattcctg cggctgtagt taatgggagg atttcacgca
     3241 ggtcgtttgg gcgttatatg aaggcgaggt tttttctgcg gcgcgtactg gcgtgtttga
     3301 gtatggcggt gatgcagtcc gaacgtgatg ctgagcgcat tcgtgccctg ggtttgtccg
     3361 ctgaatccgt gttcactgcg ggcaatttaa agttcgaatc tgggatttac acggataagc
     3421 acacggatgt gaaagaacga tttgggttgc gggcggaggt gccgctagtg ctcgcggcga
     3481 gcacgcacgc gcccgaagag aagatcgtac tcgagagttt taagcaactt cgggactcac
     3541 gccccgttcg attgatactc gcgccgcgac gtcccgaacg gttcaatgaa gttgctgagc
     3601 tgattgacga gtcgggcctg agttgggcgc gaaggacaaa cgcgccgcat gcgcgcgacg
     3661 ttgaggccga ggttatcctg ctcgatacga ttggggagtt gccggcgatc taccctttgg
     3721 caacggcggt ttttgtgggc ggcagcatcg ttgatcgcgg cggacacaac gtgctcgagc
     3781 ctgcggcaca tggcgtggcc gtaatcaccg gcgcgcacac acataacttt aacgccatcg
     3841 tcgatctgct caataacgca gacgcgatcg ttcaactgcc gccggtggaa ggagcggaag
     3901 cggtgacgag gttgacggag gtcttgcagc gcatgcttgc tgacgctgac tggcgaattg
     3961 aaataggaac gcgggcgaaa gaactcatca tcgcgaatca gggcgcagcc gagcgtacaa
     4021 tgaaactgct cgcgccgctc ttctccggaa agctgtcaga caagactcca tcagactcgc
     4081 tactgccttt gaacgcccac acttcatgaa gtcgttaatt cttcctccgc tcagtgcgtt
     4141 gtacgacgtg gtgacgcgca cgcgtctctc gctctatcgg cgcggcgcct ttcgcacgac
     4201 ccggctcgat cgcccggtca taagcgtcgg caacatcacc accggcggaa ccggaaaaac
     4261 gccgctggtc gagtggatcg cgcgaaacct tagtaacgcg ggcaggaagg tgtgcgtact
     4321 cacgcgcggt tatggacgcg aaaacccgca gagccaggtc gtcgtctctg atggtcaaac
     4381 gattctcgca acacccgcgg aagccggtga tgaaccctat ctactcgcga caaatcttct
     4441 tggttcggcg gcggtgatct gcaacgccga tcgcatcagc gccggccgct acgcaatcga
     4501 agctttcggc actgagtgtt tcgtcctcga cgacggtttt caacacctgc ggctcgcgcg
     4561 cgacctcgag atcgttacca tcgacgcaac caatccatgg ggcagcggag agtctcaccg
     4621 caatggcgga caccttctac cctacggtcg tttgcgggag ccattgtccg gtctgcgccg
     4681 cgccgactgc gtggtcttga cgcgctgcga tcaaacagac ccagctaacc tcgaagccct
     4741 gaaacaccaa atccgctctt taatcggcgg ccgtcccctc ttcgaatcga cgatgagggc
     4801 gtcccgcaaa ccagacgcac ccggcccagt cgccgcgttt tgcgccgtcg gaaaccccga
     4861 gtcgttc
//
LOCUS       DASTEY010000565         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_177706, whole genome shotgun sequence.
ACCESSION   DASTEY010000565 DASTEY010000000
VERSION     DASTEY010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177706"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFO72_09915"
     CDS             complement(<1..827)
                     /locus_tag="VFO72_09915"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9479651.1"
                     /translation="MPKYIFVTGGVVSSLGKGLAASSIGCLLESRGLRVALIKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHAHLSQANNWTTGRIYLSVIEKE
                     RRGDYLGKTIQVIPHITNEIKEAVRKVAEKDQPDVIIVEIGGTVGDIESLPFLEAIRQ
                     MGNEEGAGNALFIHVTLVPYIAAAGELKTKPTQHSVRDLREIGIAPDILLCRSEKPLS
                     RDLRGKIALFCNVKESAVISAQDVDTIYDVPLAFHEQGLDDLIVMQLGLHGQAPQADL
                     "
     gene            complement(902..1195)
                     /locus_tag="VFO72_09920"
     CDS             complement(902..1195)
                     /locus_tag="VFO72_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479652.1"
                     /translation="MNDWIGLAIIGFLILCGLFALSRLSKPYDVSNEEFERRAQEGPG
                     LLGAGLVGLQKALDPAMQKAVEAQEDLKQGRYDGEQGSGEPPEPGSTRQDEQI"
     gene            complement(1192..2028)
                     /gene="kdsB"
                     /locus_tag="VFO72_09925"
     CDS             complement(1192..2028)
                     /gene="kdsB"
                     /locus_tag="VFO72_09925"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET9479653.1"
                     /translation="MNQTGNNPQNLPTHTTVAIIPARFASTRLPGKALIELAGKPMIC
                     WVAERALAARNVDRVIVATDSEQIAQAVRAREIEVMLTSQHHTSGTDRVAEVAAAIPE
                     AEIIVNVQGDEPLIAPQTIEKTVDVMKLELRKTTGSAGIVTTWEPLQSYADLVDFNVV
                     KVVVDDDDNALYFSRSPIPFPRDAVKRHGSPDIALATEPDLLKLFRKHTGLYVYRRDV
                     LLEFTNWLPTQLERIEALEQLRALAHGVKIKVVEACTSSTGVDTLADLEKVRATLALQ
                     NA"
     gene            2089..2598
                     /locus_tag="VFO72_09930"
     CDS             2089..2598
                     /locus_tag="VFO72_09930"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9479654.1"
                     /translation="MDRTNQDGQGLIDAWRTNNRVTTYLIENLPPELWSKSVPGSPRR
                     TVRTIAAHIHNTRCMWIKMIGARHGVATPRTVNVRTVRPPELLRALDRSSKGIEDLIR
                     LGVANGGAVPPAAWQNFPTDLMHFLSYFVAHEAHHRGQLCMLARQLGHRLPADVTAGL
                     WQWKKRSQE"
BASE COUNT          621 a          799 c          809 g          640 t
ORIGIN      
        1 agatctgctt gcggcgcctg tccatgcaaa cccaattgca tgacgatcag atcgtcgagt
       61 ccctgctcgt gaaacgcgag cggcacgtcg tagatcgtgt caacgtcctg cgcgctgatc
      121 accgccgatt ctttaacgtt gcagaagagc gcgatcttgc cgcgcagatc gcgtgacaac
      181 ggtttttctg aacggcacag aagaatgtcg ggcgcgatac cgatctcgcg caagtcgcgc
      241 acggagtgtt gcgtcggttt ggtttttagt tcaccggcag cggcgatgta aggcacaagc
      301 gtgacgtgaa taaacagagc gttacccgcg ccttcttcat ttcccatctg gcgaatcgct
      361 tcgagaaacg gtaacgattc gatgtcgccg accgtgccgc cgatctcgac gatgatgacg
      421 tccggctgat ccttttccgc caccttgcgc acggcttctt tgatttcgtt ggtgatatgc
      481 ggaatgacct gaatcgtttt gccgagatag tcgccgcgac gctccttttc gatcacggaa
      541 agatagatcc ggccggtggt ccagttgtta gcttgcgaga gatgcgcgtg cgtgaagcgt
      601 tcgtaatggc cgagatcgag atctgtttcc gcgccgtcgt cggtgacgaa aacttctccg
      661 tgctggaacg gcgacatcgt gccgggatcg acgttgatgt acggatcgag tttgatgagt
      721 gcgacgcgaa ggccgcgact ctcgagcagg cagccaatcg aagaggccgc caggcccttg
      781 cccagactcg aaaccacacc acccgtgacg aagatgtatt taggcatttg gtttctccca
      841 agttgacagg attacagaat taccacgaag gcaggagaga gttacttttg ccacagtcct
      901 gttaaatctg ttcatcctgt cgagttgatc ccggttcggg cggctcgccc gatccctgtt
      961 caccgtcgta acgtccctgc ttcaaatcct cctgcgcttc cacagctttt tgcattgccg
     1021 gatcgagggc cttctgcaaa ccaaccaacc ccgcaccgag caaaccgggt ccttcctgag
     1081 cgcggcgttc aaactcttcg ttcgaaacgt cgtaaggctt gctgagtcgc gacagagcaa
     1141 acagtccgca caaaatgagg aaaccaatta ttgccagtcc gatccagtcg ttcatgcgtt
     1201 ttgcaaggca agagtcgctc ttaccttctc caggtctgca agcgtgtcaa cgcccgtcga
     1261 agaagtgcac gcttcgacta ccttaatctt cacaccgtgc gcgagggcgc gcaattgttc
     1321 gagtgcttcg atccgttcga gttgcgttgg cagccagttg gtaaactcca gcaacacgtc
     1381 tctgcgatag acgtagaggc cggtgtgctt gcgaaaaagc ttcaatagat ccggctctgt
     1441 tgcgagtgca atgtcaggcg agccgtggcg cttgacggcg tcgcgtggaa acggtatcgg
     1501 tgagcgtgaa aaatagagag cgttatcatc gtcgtcaacg actaccttta cgacgttgaa
     1561 atccaccaag tccgcatacg actgaagcgg ctcccaagtg gtcacaatgc ctgcgctacc
     1621 ggttgttttc cgcaactcca acttcatgac gtcgacggtc ttttcgatcg tctggggcgc
     1681 gatcaacggc tcgtcgccct gcacgtttac gataatttcg gcttctggta tcgctgccgc
     1741 gacttccgcg acgcgatcgg tgccactggt gtgatgctga ctcgtcaaca tcacttcaat
     1801 ttctcgcgcg cggactgctt gagctatctg ttcgctgtca gtagcgacga tgacgcgatc
     1861 gacgttacga gcggcgagtg cacgttcagc tacccaacag atcatcggtt ttcccgccag
     1921 ttcgatcaat gccttgccgg gcaaacgagt agaagcgaaa cgcgcgggaa tgatggcgac
     1981 tgtcgtgtgt gtggggaggt tttggggatt gttgcccgtc tgattcacgg gataacagct
     2041 tagcctgcgg gtgcaaaaac tgtcaacgaa agttaatctg tgtcagccgt ggatagaacg
     2101 aaccaagatg gccaagggct gatcgatgcg tggcggacga acaatcgcgt cacgacctat
     2161 ttaatcgaaa acttgccgcc cgagttgtgg tcgaaaagtg ttcccggatc gccgcgacgc
     2221 acagtacgaa cgatcgcggc gcatattcac aacaccaggt gcatgtggat caaaatgatt
     2281 ggcgcgcgcc atggtgttgc gacgccacgg accgtcaatg tgcgcacagt tcggccgccg
     2341 gagttgttgc gcgcactcga ccgcagcagt aaaggaatcg aggatttgat tcgtctcggg
     2401 gtggcaaatg gtggcgcggt cccgcccgcg gcgtggcaaa acttcccgac ggacctgatg
     2461 cactttctga gctacttcgt cgctcacgag gcgcatcatc gcgggcagtt gtgcatgttg
     2521 gcgcgccaac tcggacatcg actgcccgcg gatgtgacag ccgggttgtg gcaatggaag
     2581 aagcgatcgc aagagtaaaa gcgcacgata cagtttgcga acttcatacc tgggaccgca
     2641 ggcgtctcgc ctgccaatcg tcgaatcgga cgctgccgtg agttgcatta actgctgcgc
     2701 cggcgtcgct tccacttgca cgaaatcaaa acgcgactgc ctgccggcgt accactagca
     2761 cacagccaca ccactgcttc catgcaatta ggcaagacgt ataccggcaa caacttcggg
     2821 catcaggctt gccagactta tcacgagagg gaggtaaaaa ctgactatg
//
LOCUS       DASTEY010000566         6692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_276424, whole genome shotgun sequence.
ACCESSION   DASTEY010000566 DASTEY010000000
VERSION     DASTEY010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276424"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFO72_09935"
     CDS             complement(<1..190)
                     /locus_tag="VFO72_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479655.1"
                     /translation="MKPLSPDTTPDAQRKQFELMRRLSPSQKLSLAFALTDAVRDLIL
                     ADLHHRFPQANETEIRRRF"
     gene            complement(187..1335)
                     /locus_tag="VFO72_09940"
     CDS             complement(187..1335)
                     /locus_tag="VFO72_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9479656.1"
                     /translation="MIELPFFTSEHRNLAQVVAQFVAREIEPHAGVEEDVEGLARHFV
                     KLLAGAGLLNYAVAVSRLDTRALCLIREALAYSSSLADLAFVMQGLGTYAISQAAPDH
                     VREFWLARALQGKAIAAFALTEPDAGSDVASIKTAARRDGDTYVIDGRKRFISNAGIA
                     DFYTVFARTGTREDGRPALSAFVVGSKMPGFSVVEHTSLIAPHPIGEVAFNECRVPAE
                     DMVGNEGDGFRLAMETLDMFRASVGAAACGMAQRALDEAVRYASTRRQFGRLLCEHQL
                     VQEKLADMVTELDAARLLVYRAAYARDTVEARVTREASEAKLFATEAAGRIVDSAVQI
                     HGGAGLVRGSVVERLYRDVRALRIYEGTSEIQKLVIAGQLLRENRDNV"
     gene            complement(1332..2126)
                     /locus_tag="VFO72_09945"
     CDS             complement(1332..2126)
                     /locus_tag="VFO72_09945"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET9479657.1"
                     /translation="MKQPTTFLYEHDEQGIATITLNRPERLNALTFQVYRELTDTFAA
                     LRNERDVRVVVITGAGRAFCSGGDVHDIIGELFRRDMEGLLEFTRMTCELIHNMRVLP
                     KPVIASLNGTTAGAGACIALASDLRIASEDARIAFLFVKVGLAGTDMGASHLLPRIVG
                     LSKATELLYTGDFITAAEAERIGFYNRVVAADQLQTATRDFAERLARGPAFALGKTKE
                     MLNRELHMDFTAALESEAQAQAICMQHPDYREAYEAYVAKREPRFK"
     gene            complement(2123..2527)
                     /locus_tag="VFO72_09950"
     CDS             complement(2123..2527)
                     /locus_tag="VFO72_09950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9479658.1"
                     /translation="MPFTLINPESLGAPSGYSHGLLADASGKLLFISGQVAWNQKQKI
                     VSADFVEQFDRALANVLTVVKAANGQPEHVVRLVIYVTNKNEYRAQTREVGERYRRHM
                     GKHFPAMVLVEVSGLVEDEAKVEIEGMALIPA"
     gene            complement(2555..3088)
                     /locus_tag="VFO72_09955"
     CDS             complement(2555..3088)
                     /locus_tag="VFO72_09955"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9479659.1"
                     /translation="MVLNIRKRILAGFMAVLVGSIVFAQGRTPTAPVEFSLRSVDGQT
                     VTSGSLRGEVVVLAFGASWLPLSRTQLQGVRKFADEYSGRGVAVYWVSTESENTKSKN
                     YASDEQLRAFAQKYGLKVTVLRDPDGVVSKKFGVDQLPSIVIIDKEGNISGPPIGGLD
                     PQGNLAGQLASRLDKLL"
     gene            complement(3168..3788)
                     /locus_tag="VFO72_09960"
     CDS             complement(3168..3788)
                     /locus_tag="VFO72_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9479660.1"
                     /translation="MPDVAAVLLAAGRSKRMGVFKPLLPFGKQTVIESCIDYLRKGGV
                     ETIVVVLGHRAGEVRARINNLGVVCVVNPDPDSQMNASIAAGVRELPDSAKATLIALA
                     DHPAVPPTVVSKLISTWQQGGGLLIPTWQNRGGHPVLIDLRYRKELENLDPNRGLKGL
                     FESHPGDLKRVPVDSPFIARDMDTWDDYHALYKEVFGEPPPEPPER"
     gene            complement(3795..4766)
                     /locus_tag="VFO72_09965"
     CDS             complement(3795..4766)
                     /locus_tag="VFO72_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9479661.1"
                     /translation="MPKFEYHIPQTIAEAVRIMAEAGPVGQFVAGGTDLYPNMKRRQQ
                     VPKTVISVMRLAELNQITGDGAHGLRIGASVTLTDICEHPIINRNYPVVAKAARTIST
                     PILRNMGTIGGNLLLDTRCNYYDQNYEWRKGINFCLKKDGDVCWVAPGSAKCWAVQSS
                     DLVPVMVAIGAKFRLASTLGERVIDAAGFYNDDGIDYLHKRADELLIDIQLPPTNGWR
                     ASYQKLRRRGAFDFPVLGVAAWLNMDQDVVRDAKVVLGGIAPAPVAIQEAASALIGKP
                     LADEQIEAAAEAAYVKARPLDNTDFVYQWRKQMARQYTLRALRDLQT"
     gene            complement(4792..5010)
                     /locus_tag="VFO72_09970"
     CDS             complement(4792..5010)
                     /locus_tag="VFO72_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479662.1"
                     /translation="MNKKSFPRPFIFSVVEDDGENVTIEVSEEDYKRDLAAGVDEDAL
                     LKPGRYKMKRGGFLARHPDLKSKKEKRA"
     gene            complement(4988..5269)
                     /locus_tag="VFO72_09975"
     CDS             complement(4988..5269)
                     /locus_tag="VFO72_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET9479663.1"
                     /translation="MRFEWDVKKASENFAKHGVTFYEAAEVFYDPNALDDYDAEHSTN
                     EARFFIIGLSTRRLLFVVYTEPETDVVRIISARKAGRTEREIYEQEIIP"
     gene            complement(5312..>6692)
                     /locus_tag="VFO72_09980"
     CDS             complement(5312..>6692)
                     /locus_tag="VFO72_09980"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9479664.1"
                     /translation="PKRGHGTPQPRYALEVHLDKIAEQLRIDPVEIRRKHLVAPNSVT
                     ANYLRIGSMGLGLCIDKVVEGSDWKNRFSGWDPDNRKLPFGKGIGIACSSYICGAGLP
                     IYWNNMPQSGVQLRLDRQGGVCVMCGSTDIGQGSDSILAYIVAEILGIDPFDIRVVTA
                     DTDLTPVDLGSYSSRVTLMTGNAAIQAAERARELLVMAVAEKLGVPIENLSLAERRVF
                     DVENPELGVSFAEAVVLAESKFGTIGTVGSYTPPRSPGKYKGSGVGPSPAYSYSAAVA
                     EVDVNPDTGIVVVERVWIAHDIGRSINPLLVMGQVEGSVYMALGEILMEEMTYRTNRN
                     VVHKFPSLLEYKSPTTMEMCDVKTYLIEDPDPNGPYGAKEVGQGPLLPVPPAVANAVY
                     NAVGVRIDEVPITPEKVARALKDKLRGREARFGPDGVPVVTWPDPLRVLTPAEGGDGT
                     EMPHVAVHS"
BASE COUNT         1406 a         2035 c         1819 g         1432 t
ORIGIN      
        1 tgaagcgtcg ccttatctct gtttcattcg cctgcggaaa tcgatgatgc aggtcagcaa
       61 gaatcagatc gcgcacagcg tctgtgaggg caaaagcgag cgagagtttc tgcgagggcg
      121 acaaacggcg catcagttcg aactgttttc gttgagcatc aggcgtcgta tctggcgaca
      181 gtggcttcat acgttatcgc ggttctccct caacaactgg ccggcgatga cgagcttttg
      241 tatttcggag gtgccctcgt agatgcgcag tgcgcgcacg tctcgataca aacgctcgac
      301 gacgctgcct cgtaccagtc cggcgccgcc gtgaatctgg acggcgctgt cgacgatcct
      361 tccggcggcc tcggtcgcga agagctttgc ttcactagcc tcgcgcgtga ctcgcgcttc
      421 gacagtgtct cgagcgtatg ccgcgcgata taccagcagg cgcgccgcgt caagttccgt
      481 caccatgtcg gccagctttt cctggaccag ttgatgttca cacagcaggc gtccgaactg
      541 tctgcgcgtc gatgcgtaac ggacagcttc gtccaacgcc cgctgcgcca tgccacacgc
      601 ggcggcgcct acactggcac gaaacatatc cagcgtctcc atcgccaaac gaaatccgtc
      661 gccttcgttg ccaaccatgt cttccgccgg tacgcgacac tcattgaaag cgacttctcc
      721 aatcggatga ggcgcgatta acgaagtgtg ttcgacaaca ctgaaaccgg gcattttcga
      781 accaaccaca aaagccgaaa gcgccggccg gccgtcctca cgcgtgcccg tgcgcgcgaa
      841 gaccgtgtag aagtcagcaa tgcccgcgtt tgaaataaaa cgttttcgtc catcgatgac
      901 gtaagtatcg ccgtcacggc gcgcggcagt tttgatcgaa gccacgtctg agcccgcatc
      961 gggttcggtc aaggcgaagg cggcaatcgc tttgccctgg agcgcgcgag caagccagaa
     1021 ctcgcgcacg tgatcgggcg ccgcctgcga gatcgcatac gtgcccaatc cctgcatgac
     1081 aaaagcgaga tccgccagcg atgaagagta agcgagtgcc tcgcgaatca gacagagggc
     1141 gcgtgtatcg agtctcgaca cggcgactgc gtagttcaac aaacccgcgc cggccagcaa
     1201 ctttacaaag tggcgcgcaa gtccttcaac atcttcttcg acgcccgcgt gcggctcgat
     1261 ctcgcgagcg acgaattggg ccactacttg cgccaggttg cggtgctcgg acgtaaagaa
     1321 cgggagttcg atcatttaaa ccttggttcg cgtttggcga catacgcttc gtacgcttcg
     1381 cgataatcgg gatgttgcat gcagatcgcc tgcgcttgtg cttcgctctc gagcgcggcg
     1441 gtgaaatcca tgtggagctc gcggttgagc atctctttcg ttttgccgag cgcgaatgcc
     1501 ggtccgcgcg cgagccgttc ggcgaaatcc cgcgtagccg tctgcaattg atccgcagcc
     1561 acgacgcgat tgtagaaccc aattcgctcg gcttcagccg cggtaatgaa gtcgccagtg
     1621 taaagaagct cggtcgcctt cgacagacca acgatgcgcg gcaacagatg cgatgcgccc
     1681 atgtcagtgc ccgcgagacc gaccttcaca aacaggaaag caatgcgcgc atcttcgctg
     1741 gcgatgcgca gatcggaggc aagtgcaatg cacgcgccgg cgccggcggt cgtgccgttc
     1801 aaacttgcaa taaccggctt cggcaaaacg cgcatgttgt ggatcagttc gcacgtcatc
     1861 cgtgtgaact cgagcagtcc ttccatatcg cgtctgaaca gttcgccgat gatgtcgtgc
     1921 acgtcgccgc ccgaacagaa cgcgcgaccc gctcccgtga tgacgactac gcgaacatct
     1981 cgctcgttac gcaacgcagc aaacgtatcc gtaagttcgc gataaacttg aaaggtcagc
     2041 gcattcaaac gctcaggtcg attgagtgtg atcgtagcga tgccttgttc gtcgtgctcg
     2101 taaaggaacg tcgttggttg cttcatgcgg gtatcaacgc catcccttcg atttcaacct
     2161 tcgcttcatc ttcaaccagt cccgaaactt caaccagcac catggccgga aaatgtttgc
     2221 ccatgtgcct gcgataacgc tcgccaactt cgcgcgtctg tgcgcgatac tcgttcttgt
     2281 tcgtcacata aataacgagc cgaaccacat gttccggctg gccgttggcc gccttgacga
     2341 ccgttaagac attcgccagc gcacgatcaa actgctcgac aaaatccgcg ctcacgatct
     2401 tctgtttctg gttccacgct acctgcccgg agatgaaaag caacttgccg cttgcgtccg
     2461 caaggagacc gtgcgagtaa ccgctcggcg caccgagtga ctcgggattg attaacgtga
     2521 aaggcatggg gagaaggtta attgttggaa gtgctcagag caacttgtcg aggcgagacg
     2581 cgagttgtcc ggcgaggttg ccttgcgggt cgagtccgcc gatgggcggt ccggaaatgt
     2641 ttccttcctt gtcgatgatc acgatcgacg gcaactgatc gaccccaaac ttcttcgaca
     2701 ccacaccgtc tggatcgcgc agtactgtta ctttgagtcc gtacttctgt gcgaacgcgc
     2761 gaagctgctc gtcgctcgcg tagtttttcg actttgtatt ctcagactca gtgctgaccc
     2821 agtagacagc cacgccgcga ccggaatact cgtcggcgaa ttttctcacg ccctgtaatt
     2881 gcgttcgcga cagtggcagc cacgaagcgc caaacgccag gacaactact tcaccgcgca
     2941 ggctgccgga ggttacagtt tggccatcga ccgaccgcag cgagaattcc acgggtgcag
     3001 tgggcgtgcg gccctgggcg aatacgatag agcccacgag cactgccata aacccggcca
     3061 gaatcctctt tcgaatgttc agaaccatct cttctccttc gccggcgtga ccgaaaaggc
     3121 tttaccggct ggcacgttag atgagtccgc ccaggctttc gttggtctta ccgttcgggt
     3181 ggctcaggcg gcggttcgcc aaaaacctcc ttgtagagtg cgtggtaatc atcccacgta
     3241 tccatgtcgc gagcaatgaa aggggagtcg acaggcactc gtttcaggtc gccgggatgg
     3301 ctttcgaaga gccctttgag gccgcggttc gggtcgagat tctctaactc ttttcgatag
     3361 cgaaggtcga tcaagaccgg gtgtccccct cgattttgcc aggttggaat aagtaatccg
     3421 ccgccttgtt gccacgtgga aatcagtttc gaaacgacgg ttggcggcac cgccggatga
     3481 tccgcgagag cgatcagcgt ggcttttgct gagtctggca gctcacgaac gccggcggcg
     3541 atcgacgcat tcatctgact gtcgggatcg gggtttacaa cacagacaac gcccagatta
     3601 tttatccgtg cccgtacttc tcccgcgcgg tggcccaaca cgaccacgat agtttcgacg
     3661 ccacctttac gcaggtagtc aatgcacgac tcgataacag tctgtttgcc gaaaggaagc
     3721 agcggcttga acacgcccat ccgtttagac cggccggcgg cgaggagtac tgctgctacg
     3781 tcaggcactg tgatttacgt ttggagatct cgcaaggcgc gcagcgtgta ttgccgcgcc
     3841 atctgctttc gccactggta gacgaaatcg gtgttatcca acggccgcgc tttgacgtag
     3901 gctgcttccg cggcggcctc gatctgttcg tcggccagcg gttttccgat caacgcactc
     3961 gcagcttcct gaatcgcaac cggagccggc gcaattccgc caagcacaac ctttgcgtcg
     4021 cgtaccacgt cttgatccat gtttagccac gccgcgacgc cgagcacagg gaaatcaaac
     4081 gcgccgcgcc gtcgcaattt ctgatacgaa gcgcgccagc cgttggtagg cggcagttga
     4141 atgtcgatta agagttcgtc cgcacgcttg tgcagatagt cgatgccatc gtcgttataa
     4201 aaacctgccg catcgataac gcgttcgccg agcgtggacg cgagccgaaa cttcgcgccg
     4261 atcgcaacca tcaccggaac cagatccgac gactgcacag cccaacactt cgcgcttccc
     4321 ggcgcgaccc agcaaacgtc gccatccttt ttgaggcaaa aatttatccc tttgcgccac
     4381 tcgtagttct gatcgtagta gttgcagcgc gtgtcgagta gcaggttgcc gccgatggtg
     4441 cccatgtttc tcagtatcgg cgtggagatg gtgcgtgcgg ctttggctac gactggataa
     4501 ttacggttga taatcggatg ttcgcagatg tctgtgagtg tgacggaagc gccgatgcgc
     4561 agaccatgcg cgccatcgcc cgtgatctga ttcaactcgg caagacgcat caccgagatc
     4621 acagtcttcg gcacctgttg ccgccgtttc atgtttggat acagatcggt gccaccggcg
     4681 acaaactgtc ctactggacc agcctccgcc ataatcctca ccgcctccgc aatcgtctgc
     4741 ggaatgtgat attcaaattt gggcaaccgc atcatcgttt gtaatccgcc tttacgccct
     4801 cttctccttt ttcgacttca gatctggatg gcgcgcgaga aaaccgcccc gcttcatctt
     4861 atagcgacct ggcttcagga gcgcatcttc gtcgacgcca gccgccaaat cgcgtttgta
     4921 atcttcctcc gagacttcga tagttacatt ttcaccgtcg tcttccacca cggagaaaat
     4981 aaacggccta gggaatgatt tcttgttcat aaatttcacg ctctgttcta ccggcttttc
     5041 gcgccgagat gattcggact acatccgttt cgggctccgt gtaaacaaca aacagcaagc
     5101 gccgagtcga cagtccaatg ataaagaacc gggcctcgtt tgttgaatgt tcggcgtcgt
     5161 aatcgtctaa ggcattagga tcgtaaaaca cttccgcggc ctcgtagaac gtgaccccgt
     5221 gctttgcaaa attctccgac gccttcttga cgtcccattc gaatctcata gtgaggattg
     5281 attcgaagcg ctctcattct tgttacccag ctcacgaatg cacggctacg tggggcatct
     5341 ccgtgccgtc accgccttcg gcgggtgtca ggacgcgcag ggggtcgggc caggtgacca
     5401 cggggacgcc gtcggggccg aagcgggcct cgcggccgcg taatttgtct ttcagtgcgc
     5461 gcgcgacttt ttccggggtg atgggaactt catcaatacg taccccaacc gcgttgtaga
     5521 ccgcgttcgc caccgccggc ggcaccggca acaacggccc ttgcccgact tccttcgcac
     5581 cgtatggtcc gttcgggtcc ggatcctcaa tcagatacgt cttaacgtcg cacatctcca
     5641 tcgtcgtggg actcttgtac tccagcagcg acggaaactt gtgcacgacg ttgcgattcg
     5701 tcctgtacgt catctcttcc atcaggatct cgcccagcgc catgtaaaca ctgccttcaa
     5761 cctgacccat cacgagcagc ggattgatcg aacggccgat gtcatgcgca atccaaacac
     5821 gctcgacaac gacgatgccg gtgtccgggt ttacgtcgac ttccgcaacc gcggcggagt
     5881 aactgtacgc cggcgatggt ccaacacccg aacctttgta cttaccgggc gaacgcggcg
     5941 gcgtgtacga tccaaccgta ccgatcgtgc caaacttcga ctcagccaac accaccgcct
     6001 cggcaaacga aacaccaagc tccggattct ccacgtcaaa cacacgacgc tcagccagcg
     6061 acaagttttc aatcggcaca ccgagttttt cggcaaccgc catcaccagc aactcacgcg
     6121 cgcgctccgc cgcctgaatc gccgcgttgc cggtcatcag agtgacgcgg ctcgaataac
     6181 taccgagatc gaccggcgtc aagtccgtgt ctgccgtaac gacgcgaatg tcaaagggat
     6241 ctatcccgag aatctcagcg acgatgtagg cgagtatcga atccgatccc tgaccaatat
     6301 ccgtcgatcc gcacatcaca caaacaccgc cctggcggtc aagtcgaagc tgtacacccg
     6361 actgcggcat gttgttccaa tagatgggca accccgcccc gcagatgtaa gaactgcacg
     6421 cgatgccgat gcccttgccg aacggaagct tgcgattatc cgggtcccaa cccgaaaaac
     6481 gattcttcca gtcggaacct tcgacgacct tgtcaatgca cagccccaaa cccatgctgc
     6541 cgatgcggag gtagttggcg gtgacggagt tgggcgccac cagatgtttg cgccggatct
     6601 caaccggatc gatacgcagt tgctcggcga tcttgtcgag atgaacttcg agcgcgtaac
     6661 gaggctgcgg ggtgccgtga ccgcgtttcg ga
//
LOCUS       DASTEY010000567         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1215892, whole genome shotgun sequence.
ACCESSION   DASTEY010000567 DASTEY010000000
VERSION     DASTEY010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215892"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFO72_09985"
     CDS             complement(<1..191)
                     /locus_tag="VFO72_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479665.1"
                     /translation="MSAAHVNVNRDSFGHVYMWSLVTAGAAIVLASLFVLPFRDLDPG
                     FVFLCLMVMASSLVAIKIP"
     gene            complement(269..2167)
                     /locus_tag="VFO72_09990"
     CDS             complement(269..2167)
                     /locus_tag="VFO72_09990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET9479666.1"
                     /translation="MKRFLYPMLWSGFVSLLLFSLFVYERRTHASATEDIQIQSKISR
                     DILKQVENGKGAELIRVIIQPAGQADLVLDSTLEYAGSDIRKLKSLQTRIVTVPAQAA
                     VALASRADVAYVSLNRDARPMGHVSSTTGADQVRATPNTISSTLDGTGIGIAVLDSGI
                     DTEHRAFLDRSNFLRVVYSEDFTGEGRVDDPYGHGTHVASLAAGNGRISNAQYVGIAP
                     NANLINLRVLNSEGLGTTAQVLRALDWVATNRVAYNIRVVNMSLGMPAVDSYRNDPIC
                     RAVRRLVDAGVVVFAAAGNNGKDSEGNKVYGHIHSPGNEPSAITVGAANTFGTNGRDD
                     DGVASFSSRGPTRSFITDEQGVNHYDNIIKPDLVAPGNKLIEAQAADNYLVRQNPSLD
                     AGVSGADERKMMFLSGTSMATPVAAGAAALMLQANPNLTPNLVKALLMYTAQPLAGYN
                     MLEQGAGQLNVAGAVQLAKLVRNDLSSATVGAPLLTVDVPPAPQTTIANYTFTWSRGI
                     VLNHTYATGDDLIRLYQLVYAPGNLLGSGVITGDGVLLLDPAFFSNGVITGDGAAFLA
                     VSLLFQNLLSEGSYVSDGVITGDGVITGDGVITGDLVTSAQSAMMNGDNSSMMTAASD
                     DGSDCLDY"
BASE COUNT          495 a          626 c          640 g          493 t
ORIGIN      
        1 ctgggtattt tgattgcgac gagcgaactc gccatgacca tcaggcagag aaagacaaag
       61 ccgggatcca ggtcacgaaa cggtaagaca aacagtgacg cgagtacgat cgctgctccc
      121 gcggttacca gcgaccacat gtacacatgc ccaaagctat ctcgattaac atttacatgt
      181 gccgcactca ttgttacctc gagaagactg gtcggtttgg agagcactat tggagtgcgt
      241 cgatagatca acgcactcca aacgaaaact aataatcgag gcagtcagaa ccatcgtccg
      301 atgctgccgt catcatcgaa ctgttgtcgc cattcatcat tgctgattgg gcgcttgtta
      361 ccaggtcgcc ggtgatcaca ccgtcgccgg taatcactcc atctccggta attaccccat
      421 cgctaacgta actaccttca gacaagagat tttgaaacag caagctcacg gccaaaaacg
      481 cagcgccatc gcccgtgatc acgccgtttg agaagaacgc cggatcgagt aacagaacgc
      541 cgtcacctgt aatgacgccg ctgccgagta aattgccggg cgcgtagacg agttgataga
      601 gccggatcag gtcatcgccg gtcgcgtacg tgtgattgag aacgatgccc cgcgaccagg
      661 tgaaagtgta atttgcgatc gtcgtctgcg gcgccggcgg aacgtcgact gtcaacaaag
      721 gcgcaccaac agttgcagac gatagatcat ttcgaaccaa cttcgccagt tgcacagcgc
      781 cggcgacgtt cagttgtccc gcgccctgct cgagcatgtt gtagccggca agaggttgcg
      841 cggtgtacat caacaacgct ttgacgaggt tcggtgtcag gttcggattc gcttgcagca
      901 tcaacgccgc cgctcccgcg gcgaccggtg tggccatcga agtgccgctc aggaacatca
      961 tcttgcgctc gtcagcgcca ctgactcccg cgtccagcga cgggttttgt ctcacgagat
     1021 aattatcggc ggcctgtgct tcgatcaact tgttgccggg cgcgacaagg tcgggcttga
     1081 tgatgttgtc gtagtggttt acgccttgtt cgtcggttat gaaacttcgc gttggaccac
     1141 gcgaactgaa cgacgcgact ccgtcatcgt cccgcccatt cgtgccaaac gtattcgcgg
     1201 cgccgacagt gatcgccgaa ggttcgttac cgggagaatg tatgtgtccg tagactttgt
     1261 ttccctcgct gtctttaccg ttatttcccg cggctgcaaa aacgacgacg cccgcatcga
     1321 ccaaccgtcg cacagcacga cagatgggat cgttacgata cgagtcaacg gcgggcatgc
     1381 cgagactcat gttcacgaca cgaatgttgt aggcgacgcg attggtagcg acccaatcga
     1441 gtgcgcgcag cacctgtgcg gtggtgccga gtccttctga gttaaggacg cgcaggttga
     1501 tcaggtttgc gtttggagca atacccacgt attgcgcatt ggagatgcgt ccgttgcctg
     1561 cggccagcga ggctacgtga gttccgtgcc cgtaaggatc gtcgacgcgt ccttcacccg
     1621 tgaagtcttc actgtacacc acgcgcagga agtttgatct gtctagaaat gcgcgatgtt
     1681 ccgtgtcgat gccggagtcg agaactgcga taccgatgcc ggtaccgtcg agcgtgctgc
     1741 tgatggtgtt gggagtcgct cttacttgat cggcgcctgt ggtcgatgag acatgaccca
     1801 tcgggcgcgc gtcgcgattg agtgaaacat aggccacgtc agcgcgcgat gcgagggcca
     1861 cagccgcctg ggccggcacc gtgacgatgc gcgtttgcaa gctcttgagt ttgcgaatgt
     1921 cgctgccggc gtattctagt gtcgaatcca gcaccaggtc ggcctggccg gcgggctgga
     1981 tgatgacgcg gatgagctca gcccccttcc cattctcgac ctgcttcaga atgtctcttg
     2041 aaatcttact ttgtatctga atgtcctcgg tggctgacgc gtgggtcctt ctttcataaa
     2101 cgaatagtga gaaaagaagg agtgagacga aaccactcca cagcatcggg taaagaaagc
     2161 gcttcatgct gaacaactcc ggtaaaccgc gctgaggtcc acttagggct acgacgtcga
     2221 aggtgaagga aattcacggg caatttcttg cacc
//
LOCUS       DASTEY010000568         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_260198, whole genome shotgun sequence.
ACCESSION   DASTEY010000568 DASTEY010000000
VERSION     DASTEY010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260198"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="sdhB"
                     /locus_tag="VFO72_09995"
     CDS             <1..207
                     /gene="sdhB"
                     /locus_tag="VFO72_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol; the
                     catalytic subunits are similar to fumarate reductase;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HET9479667.1"
                     /translation="VRLFNMHPTGKMNREERLEGIMQEDGITNCGNAQNCVKACPMNI
                     PLTRAIYEENRETVIHGLFGWLKG"
     gene            complement(225..1157)
                     /gene="mdh"
                     /locus_tag="VFO72_10000"
     CDS             complement(225..1157)
                     /gene="mdh"
                     /locus_tag="VFO72_10000"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9479668.1"
                     /translation="MGARKKITVVGAGNVGATAAHWLAAKELGDVVLVDIIEGVPQGK
                     ALDLAQAAPIEGFDVSLIGANNYDQTDNSDVVIITAGLARKPGMSRDDLLKTNADIVG
                     KVVDEVVRRSPNTILIIVSNPLDAMCQVALRRSGFPKHRVIGMAGVLDSARMRCFLAE
                     ALDVSVENVTAFVLGGHGDTMVPLPRFSTCAGIPITELLPKDQINAIVKRTAGGGAEI
                     VSLLKTGSAYYAPSAAAVEMTESILKDKKKILPCAAYLEGEYGINGLYVGVPCKLGAR
                     GLEQVIEINLTAEERIALHKSASAVQELVDVLGI"
     gene            1240..1530
                     /locus_tag="VFO72_10005"
     CDS             1240..1530
                     /locus_tag="VFO72_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9479669.1"
                     /translation="MDPEDPFPEPVEIEITDVFDLHTIPPRDVRRVVEEYLEIAREKG
                     FPVVRIIHGKGIGVQREMVRAILARTDYVLDWTDAPPEAGGLGATIVRLKAV"
     gene            1541..1879
                     /locus_tag="VFO72_10010"
     CDS             1541..1879
                     /locus_tag="VFO72_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479670.1"
                     /translation="MLVLLAILIFVYHFASGFYLLMGVEPSPAVEFLYIGASACASVW
                     WLKSETRHSAAKSVYCKGLLVGAYWPLVLPYHLLKTRGWKGLLPLLLLIASYLFSQVL
                     VFALYFATME"
BASE COUNT          422 a          527 c          589 g          468 t
ORIGIN      
        1 gtgcgtctct tcaacatgca tccgactgga aagatgaacc gcgaggaacg tttggaaggc
       61 attatgcaag aagacgggat taccaactgt ggcaacgcgc agaactgcgt gaaggcgtgt
      121 ccgatgaata tcccgctgac gcgggcgatc tacgaagaga atcgagagac cgtgatacac
      181 ggtctcttcg gttggctgaa gggctaatcg gttttatccg tggcctaaat tcccaacacg
      241 tcaacgagtt cctggaccgc ggacgcgctc ttgtgcagcg cgattcgttc ctcggcagtc
      301 aggttgatct cgattacctg ctccaagccg cgcgcgccga gtttgcacgg cacaccaacg
      361 tagagtccat tgatgccata ttcaccttcg agataagcag cgcacggcag gatcttcttc
      421 ttgtctttca gaatcgattc ggtcatctcg acggcggctg cggatggcgc gtagtaagca
      481 gaaccagtct tcaacaggct cacgatctcg gccccgccac cagccgtgcg tttcacgatc
      541 gcattgatct ggtcctttgg cagcaactca gtgatcggaa taccggcgca ggtggagaaa
      601 cgcggcaacg gcaccatcgt gtcgccgtgt ccgcccagca cgaacgcagt cacattttca
      661 accgacacgt cgagagcttc ggcaaggaag catcgcatgc gagcggaatc gagcacaccg
      721 gccatgccga ttacgcgatg tttgggaaaa ccggaccgtc tgagggcaac ctggcacatc
      781 gcgtcgagtg ggttcgaaac gatgatcaaa atcgtgttgg gcgaacggcg aacaacttcg
      841 tctacgactt tgccgacaat gtcggcgttg gttttgagca gatcatcgcg gctcatgccg
      901 ggtttgcgcg ccaggcccgc agtgatgatc accacatcgg agttgtcggt ttgatcgtaa
      961 ttgttggcgc cgatcaggct cacatcgaag ccttcgattg gagctgcctg ggccaggtcg
     1021 agagcttttc cctgcggaac gccttcaata atatcgacga gcacaacgtc gccgagttcc
     1081 ttcgcggcga gccagtgtgc cgcggttgcg ccgacgtttc ccgcgccgac aacggtgatc
     1141 ttctttcgcg cacccataga attatttcct ttggattaag atgttaggat ttgaaagaac
     1201 gcgagtctac aagcgggtta gtgaggtgtc aaatcgcgta tggatccgga agatccgttc
     1261 cctgaaccgg ttgagataga gatcacagac gtattcgatc tgcacacaat tccgccgcgc
     1321 gatgtaaggc gagtggttga ggagtatctg gagatcgcgc gtgagaaggg ttttcccgtc
     1381 gtgcggatca ttcacggcaa agggattggg gtgcagcgtg agatggtgcg ggcgattctg
     1441 gcgcgtacag attacgtttt ggattggacc gacgcgccgc cggaggccgg tggattaggc
     1501 gccaccatcg tgagactaaa agctgtataa tcccgggcgc atgctggtcc tcctggccat
     1561 cctcatcttt gtctaccatt tcgccagcgg tttttacttg ctgatgggag ttgaaccatc
     1621 gccggcagtt gagtttcttt atattggggc gtctgcgtgc gcttcggtgt ggtggctgaa
     1681 gtctgagacg cgccattctg ccgcgaagtc agtttactgt aagggtctgt tggtcggcgc
     1741 gtattggccg ttggtccttc cttaccactt gctaaaaacg cgtgggtgga agggcctgct
     1801 gccgttgctg ctgctaatcg cgagttatct tttctcgcag gtactggtgt tcgcactcta
     1861 cttcgccact atggagtaat gaagttgtaa cgcagaacgc ctactacttt tacaggcatc
     1921 ccgtccaatg tagtcgtact aaacaacgag tctcgcgctg ctgtgagaca agcagactga
     1981 agcaggggat ggccactgac ggcgct
//
LOCUS       DASTEY010000569         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_243425, whole genome shotgun sequence.
ACCESSION   DASTEY010000569 DASTEY010000000
VERSION     DASTEY010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243425"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFO72_10015"
     CDS             complement(<1..313)
                     /locus_tag="VFO72_10015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HET9479671.1"
                     /translation="MSQSTATDKQLTAAELYLDLLKKCLTRSIFGEKYRSLPPDDSNL
                     FIKTVRKWLAHKNLQIVRATNFDRHAREEGLDWPEEGETMIGLRRLQNLQDCITHVVQ
                     RN"
     gene            complement(291..935)
                     /locus_tag="VFO72_10020"
     CDS             complement(291..935)
                     /locus_tag="VFO72_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9479672.1"
                     /translation="MSTGGELLQALKAVSQTIPVGDPAEAVLRPVATAAANLNRNDVR
                     VLTEWRNRYVTSFLTEFAASEARTERWLTKTVGPDPTRILFMLDDLNGHTLGYLGLAF
                     IDWEKRTGEADAIVRGVDSGPGLMSRALLTLLGWAHQQLGLTTSLSVRVRSDNPAALR
                     FYLKFGVETRRVSLREVAEPDMRRWVEDESLSVSPVQLVYVSFHRHLYESIYRH"
     gene            complement(942..2069)
                     /locus_tag="VFO72_10025"
     CDS             complement(942..2069)
                     /locus_tag="VFO72_10025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9479673.1"
                     /translation="MKIPVFKPLIEKEEIDASREALELGWLGMGSYVGTFEERIKNYL
                     AAHDRYVAAVSTGHAAIHLGLLTMGVGNRDEVITPSFNNAADFQAILATGAEPVFCDI
                     DDNSLCIDLDKADELVSPRTKAIIVMDYDCVLCDHDRVADFARVHGLRVLHDAAHSFG
                     SKYKKRAIGSFSDVATFSFDPVKTITCIDGGALVLRGEEELARVREMRLIGMNQPVRV
                     MYTNERAWTYDMQTIGFRYHMANLHAAIGLAQLEKIERISESRNFACRYYNEQLRDIP
                     EVKVPQTDFAGITPFLYYIRVPGKKREALRAHLRERGIDTGIHWQPGHWFTLLKHCRR
                     GDLSVTDRVGHEVLSLPLHSMMKTDTLDSVITEIRDFFSAR"
BASE COUNT          477 a          624 c          614 g          510 t
ORIGIN      
        1 tgtttcgctg gaccacgtga gtaatgcagt cctgaaggtt ttgcaggcgc ctcagaccga
       61 tcatggtttc accttcttcg ggccagtcga gaccttcttc tctcgcatgg cggtcgaagt
      121 tagtggcgcg cacgatttgc aggtttttat gcgccagcca cttgcgcact gttttaataa
      181 acaggttgct atcgtcgggc ggcagcgagc gatacttttc gccaaaaatg gaccgcgtga
      241 ggcatttctt cagcaagtcg agatataact cggccgcagt tagctgtttg tcagtggcgg
      301 tagattgact catacagatg gcgatgaaaa cttacataaa ccagttgcac tggactcact
      361 gacaacgact cgtcctcaac ccaacgccgc atgtccggtt ctgcgacttc ccgcagcgat
      421 acacggcgag tttcaactcc aaacttgaga tagaaacgga gtgcggctgg attgtccgag
      481 cgcacgcgaa cgctcaaact ggtcgtcaat ccaagctgct ggtgggccca cccgagtagc
      541 gttagcaatg ccctgctcat gaggcccggc ccgctgtcca caccgcgaac gatggcgtcg
      601 gcctcgccgg tccttttttc ccagtcgatg aacgcgagac caagatagcc caatgtgtgc
      661 ccgttaagat catcgagcat gaacagaatt cgcgtggggt ccggcccgac tgtcttcgtg
      721 agccatcgtt cggtgcgtgc ttcgctggcc gcaaactcag tcaaaaacga tgtcacgtaa
      781 cgatttcgcc actcggtcag gacgcgaaca tcgttacggt tcaagttggc ggccgcagtc
      841 gcgaccgggc gcaacacagc ttcagcggga tctccgacag gaatcgtttg cgaaacggct
      901 ttcaacgcct gtagtagttc gccacccgta ctcatccgtg ttcaccgtgc cgaaaagaag
      961 tcacggattt ccgtgatcac actgtccagc gtgtcagtct tcatcatcga atgcagcggc
     1021 agtgacaaga cttcgtgacc cacgcgatcc gtcacagaga gatcgccgcg acggcagtgc
     1081 ttaagcaacg tgaaccagtg acctggttgc caatggattc cggtatcgat gcctcgttca
     1141 cgcaaatgag cccggagggc ttcgcgtttc ttgccaggca cgcggatgta atagagaaat
     1201 ggtgtgatgc cggcaaaatc cgtctgcgga actttcactt ccggaatatc gcggagttgt
     1261 tcgttgtagt agcggcaggc gaaattgcga ctctcggaaa ttcgttcgat cttttcgagt
     1321 tgcgccaggc cgattgccgc gtgcagattc gccatatgat agcggaaacc gatagtctgc
     1381 atgtcatagg tccacgctcg ttcgttcgtg tacatgaccc ggacgggctg gttcatgccg
     1441 atcagacgca tctcccgaac gcgagccaac tcttcttcgc cgcgaaggac gagggcgccg
     1501 ccatcgatac aggtgatcgt cttgaccgga tcaaagctaa acgtcgcgac gtccgagaag
     1561 ctgccgatcg cgcgtttttt atacttcgag ccaaacgaat gcgccgcgtc gtgcagcact
     1621 ctgagcccat gcacgcgagc aaagtcggcc acgcgatcgt gatcgcagag tacacagtcg
     1681 tagtccatca cgatgatcgc tttggtgcgt ggcgaaacca gttcgtcggc tttatcgaga
     1741 tcgatgcaaa gtgagttgtc atcgatgtcg caaaaaaccg gttcagcgcc ggtcgcaaga
     1801 atggcctgga aatcggcagc gttgttgaac gacggtgtaa tcacctcgtc gcggttgcca
     1861 acgcccattg tgagcagtcc gagatgaatc gcggcgtggc cggtgctgac ggcagctacg
     1921 tatcgatcgt gcgcggcgag gtaatttttg atccgttctt cgaaggtgcc gacgtagctt
     1981 cccattccga gccaccctaa ctccagggcc tcacggctcg cgtctatttc ttctttttcg
     2041 atcagcggtt taaagaccgg tatcttcatt ccttaccaaa cagagtgatc tcaaaatccg
     2101 ggtaagtgcg gtcccgttcg ttcacgatca ctccctcggt ggctggccag ggaatgtcga
     2161 agaccggatc gttccatctc acgccacgtt cgcttgtggg cgcatatcgc gtcgatagtt
     2221 ggtaa
//
LOCUS       DASTEY010000570         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1347773, whole genome shotgun sequence.
ACCESSION   DASTEY010000570 DASTEY010000000
VERSION     DASTEY010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347773"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFO72_10030"
     CDS             <1..501
                     /locus_tag="VFO72_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9479674.1"
                     /translation="ENAPCEPVCPVHATVHSAEGLNDMVYNRCVGTKYCSNNCPYKVR
                     RFNFFLYQDWDTPTYQLMRNPDVSVRSRGVMEKCTYCVQRIQNGKIQAELEDRKLRDG
                     EVVTACQAVCPTEAIVFGDLNDPNSRVSKLQASERNYSLLGELNTRPRTTYLSQLKNP
                     NPEIGH"
     gene            547..1932
                     /gene="nrfD"
                     /locus_tag="VFO72_10035"
     CDS             547..1932
                     /gene="nrfD"
                     /locus_tag="VFO72_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9479675.1"
                     /translation="MQDEPHKPYTEGPMHSTAPVIEPGLTFASVTDKISSIVLKRKTS
                     LAWFFGFAISFALFQLMLLTITNLVFVGIGLWGVNVPVAWGVDILNFVWWIGIGHAGT
                     LISAILLLLRQRWRTSINRFAEAMTLFAVAQAGMYPIFHLGRPWLMYWLFPYPNTMGI
                     WPQFRSPLMWDVFAVSTYATVSALFWFVGLIPDFATLRDRARSKPFQILYGILAMGWR
                     GSARHWHRYEMAYLLLAGLATPLVLSVHTIVSFDFAVGIIPGWHATIFPPYFVAGAIY
                     AGFAMVLLLTIPLRRFYGLEGFITMRHMHNMAKVMLATGLIVGYGYVMEAFFGWYSGN
                     KYERFMIWNRMHGPYSLYYWALILCNIVTPQFLWIKKVRSNLLALWVISLIVSIGMWL
                     ERFVIVVTSLHRDYLPSSWGMYQPTQWDWGMFIGSIGFFFTLLFLFIRFLPMISIFEM
                     RTILPEAEVEE"
     gene            1937..2506
                     /locus_tag="VFO72_10040"
     CDS             1937..2506
                     /locus_tag="VFO72_10040"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET9479676.1"
                     /translation="MSTRPPLVHHRRHAPPLYGVIAEFESPNDLVHAARKVYEAGYRR
                     INGYSPYPIEELAEAIGFTHTRLPLIVFIGGLIGGIGGFFMQYWMEVIDYPLNVGGKP
                     YNSWPAFIPITFEMTVLCAAFAAVLGMLVLNKLPQPYHPVFNVPNFALATRDRFFLAV
                     EANDPQFDHAKVVELLRSLGATEVSDVEV"
     gene            2493..3140
                     /locus_tag="VFO72_10045"
     CDS             2493..3140
                     /locus_tag="VFO72_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9479677.1"
                     /translation="MSRFRNKFAIGVLLIGVCLLGGACRQDMQDQPKMKPYRGSSFFG
                     DGLSSRPPVQGTVPRGFLRSDTEFYTGKRSAPSASPAPTDTNAYPDDVDTFPFPITRE
                     TVDRGRERYEIFCSVCHGMTGYGDGMIVRRGFRRAASFHDDRLRQAPVGHFFDAITNG
                     WGAMPSYAEQIPVQDRWAIIAYVRALQLSQQNTQTAPGPSGSPTPQSTPAAGGHQ"
     gene            3140..>4207
                     /locus_tag="VFO72_10050"
     CDS             3140..>4207
                     /locus_tag="VFO72_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479678.1"
                     /translation="MSTSFNPPPTGSFQNRALIVGAVVLLVFVATAFLDARQFFQAYL
                     VGWTFWTGIAVGSLALLMLQHLTGGGWGLVIRRVLEAATRTLPIMAVLFIPVIAGAHS
                     IYEWTHPEVLEQHPVVKFKTPYLNLAFFTARAVIYFAVWIVLAWFLNRWSLEQDRTAD
                     VRYAKKMRVLSGPGMVALIFTVTFASVDWYMSLEPEWFSTIYGFIFVASWTLSALAFV
                     IAVMAILTKEEPLQRIVAPLHFHDLGKLMLAIVMLWAYFAFSQYLIIWSGNLPEEIGY
                     YIDRTHGFWGAIVVTIALLHFGAPFLFLLSRELKRNPQRLVMVALLVLIMRMIDLLWM
                     LVPAFHEHRWIWLDVLAVLGFG"
BASE COUNT          772 a         1144 c         1224 g         1067 t
ORIGIN      
        1 gaaaacgcac cgtgcgagcc cgtctgtccg gtccacgcaa ccgtacacag cgccgaaggc
       61 ctgaacgaca tggtctacaa ccgctgtgtc ggcacgaagt actgttctaa caactgcccg
      121 tacaaggtgc gccgcttcaa tttctttttg tatcaagact gggacacgcc gacttaccag
      181 ttgatgcgta atcccgacgt gagtgtgcgc agccgcggcg tcatggagaa gtgcacctac
      241 tgcgtgcagc gcattcagaa cggaaaaatt caggcagaac tagaagacag aaaactgcgc
      301 gacggcgagg tcgtgaccgc ttgtcaggct gtttgcccga ctgaagcgat tgtttttggc
      361 gacctgaacg atccaaatag ccgcgtctcg aagttgcaag cgagcgaacg gaattattcg
      421 ctgctgggcg agttgaatac acgcccgcgc acgacctatt tgtcgcagtt gaagaacccg
      481 aatccggaaa tcggccactg atttcacgaa ctattttgtg tctgtgttat cggtggcttt
      541 taagtgatgc aggacgaacc acacaagccg tacaccgaag gtccaatgca ttcgacggcg
      601 cccgtgatcg aacccgggct cacgtttgct tccgtgaccg acaagatcag ctcgatcgtc
      661 ttgaaacgca agacgtcgct ggcgtggttc tttggttttg ccatttcgtt tgcgctgttt
      721 caactgatgc tgctgacgat cacgaatctc gtgtttgtcg gtatcggttt gtggggcgtc
      781 aacgttccgg tagcgtgggg cgtcgacatt ctcaacttcg tctggtggat tggtatcggc
      841 cacgcaggca cgctgatctc ggctatcctg ctgctgctgc ggcaacgctg gcgcacctcg
      901 atcaaccgct tcgcagaagc gatgacgctt ttcgcggtgg cccaggccgg catgtatccg
      961 atctttcacc tgggccgtcc gtggttgatg tactggctgt ttccctatcc caacacgatg
     1021 ggaatctggc cgcagttccg cagcccgttg atgtgggacg tgtttgccgt ttcgacttac
     1081 gcaacggtct ccgcgctgtt ctggtttgtc ggtttgatcc cggacttcgc cacgcttcgc
     1141 gaccgcgcgc gcagcaagcc gttccagatt ctttacggaa tactcgccat gggctggcgc
     1201 ggttcggcgc gtcactggca tcgctacgag atggcgtatc tgttgctcgc cggtctcgcg
     1261 acgccgctgg tgctttcagt gcacacgatc gtcagtttcg acttcgctgt cggcatcatc
     1321 ccgggatggc acgcgacgat ctttccgccc tacttcgtcg ccggcgcaat ctacgcgggc
     1381 ttcgcgatgg tgctgttgct gacgattccg ttgcgccgtt tttacgggct ggaagggttc
     1441 atcactatgc gccacatgca caacatggcg aaggtgatgc tcgcgacggg attgatcgtc
     1501 ggttacggct atgtcatgga ggcgttcttc ggctggtaca gcggcaacaa gtacgaacgc
     1561 ttcatgatct ggaaccgcat gcacgggcct tatagccttt attactgggc gttgattttg
     1621 tgcaacatcg tgacgccgca gtttttatgg atcaagaagg tgcgatcgaa cctgcttgcg
     1681 ctgtgggtga tttcactgat cgtcagcatc ggcatgtggc tcgagcggtt cgtgatcgtc
     1741 gtaacgagct tgcatcgcga ctacttgccg tcgtcgtggg gaatgtatca gccgacgcag
     1801 tgggactggg gcatgttcat cggttcgatt gggttcttct ttacgctctt gttcctcttc
     1861 atcaggttcc tgccgatgat ctcgattttc gagatgcgca cgattctgcc ggaagcggag
     1921 gtggaggaat agcacgatgt ctactcgtcc accgctcgtg catcaccgtc gccacgcacc
     1981 gccgctgtat ggcgtgatag cggagtttga gagtccgaac gatctcgttc acgcggcgcg
     2041 aaaggtttac gaggcaggct accggcgaat caacggctat tcgccttatc cgatcgaaga
     2101 gttggcggag gctatcggat ttacgcacac gcgtctgccg ctgatcgtct ttatcggcgg
     2161 attgattggc ggcatcggtg gtttcttcat gcagtactgg atggaagtca ttgactatcc
     2221 gctcaatgtc ggtgggaagc catacaacag ttggccggct ttcattccga tcacttttga
     2281 aatgacggtg ctgtgcgcgg cgttcgcggc ggttctggga atgctggtgc tgaataagtt
     2341 gccgcaacca taccacccgg ttttcaacgt gccgaacttt gcgctcgcaa cgcgcgatcg
     2401 gttctttctg gctgttgagg caaacgatcc gcaattcgat cacgccaagg tggtcgagtt
     2461 gttgaggagt ttgggtgcga cagaagttag cgatgtcgag gtttagaaat aaatttgcga
     2521 ttggcgtcct actgattggc gtttgcctgt tgggaggggc gtgtcgccag gacatgcagg
     2581 accagccgaa gatgaagcct taccgcggct cgtccttctt tggcgatggt ctttcgagcc
     2641 ggccgccggt gcagggcacc gttccgcgtg ggtttctgcg ctccgataca gagttctaca
     2701 ccggaaaaag atccgcgcca tccgcgtctc ctgctcctac ggatacaaat gcgtacccgg
     2761 atgacgttga cacttttcct tttccgatta cgagagagac tgtcgaccgc ggacgcgagc
     2821 gttacgagat cttttgtagc gtctgtcacg gcatgaccgg ttatggcgac ggtatgatcg
     2881 tgcgccgtgg ttttcgtcgc gccgcttcgt ttcacgatga ccgcttgcga caggcgcccg
     2941 tcggccattt cttcgacgca atcacaaacg gctggggcgc gatgccctcc tatgcagaac
     3001 agattcccgt gcaggatcgc tgggccatca ttgcctacgt ccgcgcattg caactgagcc
     3061 agcaaaatac gcagaccgct cccgggccaa gcggttcgcc aacgccgcaa tcgacaccag
     3121 ccgccggagg acaccagtga tgagtacatc attcaatcct ccgccaaccg gatcatttca
     3181 aaaccgtgcg ctgattgtcg gcgctgtggt gctgctggtg tttgtcgcga ctgcatttct
     3241 ggatgcgcga caatttttcc aggcttatct cgtcggctgg acgttctgga ccggaatcgc
     3301 ggtgggatcg ttggcgctct tgatgctgca acacctgacc ggcggcggct ggggtcttgt
     3361 gattcgtcgc gtgctcgaag ccgcaacgcg aacgttgcca ataatggcgg tgttgtttat
     3421 tcccgttatc gccggtgcgc actcgatcta cgaatggacg caccccgaag tgttagagca
     3481 acacccggtg gtgaagttta aaacaccgta tctcaatctc gcgttcttta ccgcgcgtgc
     3541 agttatttac ttcgctgttt ggatcgtact cgcgtggttt ttgaatcgct ggtcgctcga
     3601 acaggaccgc actgccgatg tgcgttacgc gaagaagatg cgcgtgttga gcggcccggg
     3661 catggtcgcg ctgatcttca ccgttacttt cgcgtccgtc gactggtaca tgtcgctcga
     3721 gccggaatgg ttctcgacga tttacgggtt tatttttgtt gcttcctgga ctctgagcgc
     3781 actcgcattc gtgatcgccg tcatggcgat tctcacaaaa gaggaaccgc ttcagcgaat
     3841 cgtcgcgccg cttcattttc acgacctggg aaaactaatg ctcgcgatcg tgatgctctg
     3901 ggcctacttc gcgttttcgc aatatttgat tatctggtcc ggtaacttgc ccgaggagat
     3961 tggttattac attgaccgca cgcacgggtt ctggggtgcg atcgtcgtta cgattgcact
     4021 gttgcacttc ggcgcgccgt tcctgttcct gctttcgcgc gagctgaaac gaaatccaca
     4081 aaggctggtg atggtcgcgt tgctggtttt gataatgcgg atgatcgatc tgctctggat
     4141 gctggtgcct gcgtttcatg agcatcgctg gatctggctt gatgtgctcg cggtccttgg
     4201 attcggc
//
LOCUS       DASTEY010000571         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_78782, whole genome shotgun sequence.
ACCESSION   DASTEY010000571 DASTEY010000000
VERSION     DASTEY010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78782"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1524
                     /gene="lysA"
                     /locus_tag="VFO72_10055"
     CDS             88..1524
                     /gene="lysA"
                     /locus_tag="VFO72_10055"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET9479679.1"
                     /translation="MPVIATSCPAWSITGFLEVRNGHLNIDGVDVLDLVAQYSSPLFV
                     FSERRIRNNIGRLQHAASVVDRPIKFCYASKANSNMAILKAVLEAGIDVEVNSGGELF
                     KALRVGFRPDQIIFNGTSKSDDELDDAVRAGIYAINVDSMYEIELVERSVARLQSQGE
                     QVAPARIALRLVPEIGTRSHLGLQTALLTSKFGISSSEVLAAFRRGLQKPDLVKVCGI
                     HIHVGSQTPDVEPFAEAFKSMWEHLVTIHNETGHTLEHINLGGGIPVNYLRDRSQADE
                     LPEHERDMLGAELEPAVVLSEALRVARESARAAKAEHLLDQVTILLEPGRSIIADAGL
                     VLTTVRNIKTRPETGDVWLLTDAGYNIMLSMNNYKWYYHLISASRADAPYKREYKVAG
                     PLCDSGDVYFDIEREGRLPDYRKLPERVVPGEVLALLNCGAYSLAQMFHYNGRPLPAA
                     VLINDHAQPELIRRRDTYEDLIANDTGY"
     gene            1595..2995
                     /locus_tag="VFO72_10060"
     CDS             1595..2995
                     /locus_tag="VFO72_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET9479680.1"
                     /translation="MQSEHTQLVRGLGLIAAISINVANVIGTGVFLKARVITCNVGSP
                     GKALMVWVFAGLLSLAGALTYAELLAMMPRAAGEYGIIRDAYGRLFGFIYGWTTFLIA
                     RTASAAALAIGFAIFLNDLLGGVLKQTYFEYTLPGGHHLSFGRLQLVALSAIIVTTLI
                     NCAAVRVSGGVATLLSGLKVVLLVGIGVGAFLYSDGNWGNLGMANTGGACEDVAITTG
                     GFVGLAAGMLGALWAYDGWNNLTFLAGEVKNPGRNLPLALIGAGLLIMALYLFINVSY
                     YHVLTPTQIADVPASSSVAAEVVRRLLGSVAVTLMAAAMMTSSFGALHASLLATSRVP
                     YALAKDGLVVKSLAHVSKTHVPVRALIVLCVWACVVALSGSFDTLTDYAIFALTLFYA
                     LVAASVFIFRRKMPEAERTYRVWGYPFVPIIFLTVSAWLIVTTIYNTPRQSAIGLGLI
                     MLGVPVYYLLDRRRST"
BASE COUNT          593 a          881 c          881 g          734 t
ORIGIN      
        1 tgggctggat cttgcgaacg ccttcggcgt aatttttgtg cgaagcatca caacatactt
       61 aaaaaactga acgctaactg atcatttatg cctgtaatag ctacctcctg ccccgcatgg
      121 tccatcaccg gttttctcga agttcgcaac ggtcatttga acatcgacgg cgtcgacgtg
      181 ctggatttag tcgcgcaata cagttctccg ctgttcgttt tctccgaacg ccgcattcgc
      241 aacaacatcg gccggttgca acatgccgcg agcgtcgtcg atcgccccat caagttttgc
      301 tacgcgtcga aagcaaactc aaacatggcg atcctcaaag ccgtgctcga ggcgggcatc
      361 gacgttgagg taaatagcgg cggcgaactc ttcaaagcgt tgcgcgttgg ctttcgcccg
      421 gatcagatca tcttcaacgg caccagcaag tcagacgacg agctggacga cgccgttcgc
      481 gcgggaatct acgcgatcaa tgtcgattca atgtacgaga tcgaactggt agaaagatct
      541 gtcgcgcgac tgcagtcgca gggcgaacaa gtcgctccgg cgcgcatcgc gttgcgactg
      601 gttccggaga tcggaacgcg ctcgcatctc ggtttacaaa ccgcgctgct cacgtcgaag
      661 tttggtatct cctcgtcgga agttctcgcc gctttccggc gtggcttgca gaaaccggat
      721 ctcgtaaagg tttgcggcat acacattcac gtcgggtcgc agactccgga cgtcgagccc
      781 ttcgccgaag cattcaagag catgtgggaa cacctggtca cgattcacaa cgaaaccggt
      841 cacacgctcg aacacatcaa tctcggcggc ggcatcccgg tgaactatct gcgcgatcgt
      901 tcgcaggcgg acgagcttcc ggaacacgag cgcgacatgc tcggcgccga gctggaaccc
      961 gccgttgtgc tcagcgaagc gttgcgagta gcgcgtgaat cagcccgtgc ggcaaaggcg
     1021 gaacacttac tggaccaggt gactatcctt ttggagcccg gccgcagcat catcgctgac
     1081 gcgggactcg tgctaacgac ggtgcggaac atcaaaacgc ggccggaaac cggcgacgtg
     1141 tggctgctca ctgacgccgg ttacaacatc atgctctcga tgaataatta caagtggtac
     1201 tatcatttga tttcagcttc gcgagcggat gcgccctaca agcgcgagta caaggttgcc
     1261 ggtccactct gcgattcggg cgacgtttat ttcgatatcg aacgcgaagg ccgtctaccg
     1321 gattatcgca aactccccga gcgtgtcgtt cccggcgaag tgttggcctt actcaattgc
     1381 ggcgcctatt cgctggccca aatgtttcac tacaatggcc ggccgttgcc cgctgctgtg
     1441 ttgattaacg accacgcgca gcccgaacta atccgtcgcc gcgacaccta cgaagatctg
     1501 attgctaacg acaccgggta ttagccacag atttacacag acttacacgg atgagggaga
     1561 cccgtgttca tctgtgtagt ctgtggccac aactatgcaa agtgagcaca cccaactggt
     1621 ccgcggtctg ggactgatcg cggcaatctc gatcaacgtc gccaacgtta tcggcaccgg
     1681 cgtgtttctc aaagcgcgtg tcatcacgtg caacgtcggc agtccgggaa aagcgttgat
     1741 ggtttgggtc tttgccggac tgctttcgct cgccggcgcg ctcacctacg ctgagttgct
     1801 cgcgatgatg ccgcgtgctg cgggtgagta tggcatcatc cgcgacgctt atgggcgttt
     1861 gtttggtttt atctacggtt ggaccacgtt tctcattgcc cgtactgcgt cagcagcagc
     1921 gctcgcaatc ggcttcgcaa tcttcctcaa cgatcttctc ggcggcgtgc tcaagcaaac
     1981 atacttcgag tacacgctgc ccggtggaca tcatctttcg tttggccggc tgcaactcgt
     2041 agcgcttagc gcgatcatcg tcacgacgct tatcaactgc gcggctgtgc gtgtgagtgg
     2101 cggcgttgca accctgctca gtggactgaa agtcgtgctg ctcgtcggta tcggtgtcgg
     2161 cgcttttcta tacagcgacg gcaattgggg caaccttggg atggctaaca cgggcggcgc
     2221 ttgcgaagac gtggccatca caaccggcgg ctttgtcgga ctcgcggctg gaatgctcgg
     2281 cgcgctttgg gcgtacgacg gatggaacaa tctcactttt ctcgccggtg aagtgaagaa
     2341 tcctgggcgc aatctaccgc tcgcgctgat cggcgccggc ttgctgataa tggcgcttta
     2401 cctctttata aacgtgagct actaccacgt gctcacgccc acgcagatcg cggatgtgcc
     2461 tgcctcttct tcggtggcgg cggaggtggt gagacgatta ctcggttcgg tagcagtaac
     2521 gttgatggcc gcagcgatga tgacgtcttc cttcggcgcg ctgcacgcat cgctgcttgc
     2581 gacttcgcgc gtgccttacg cgttggcgaa ggacggtttg gtcgttaaga gtcttgctca
     2641 cgtgtcgaag acgcatgtgc cggtgagggc gttgatcgtg ctttgtgttt gggcgtgtgt
     2701 cgttgctttg tcaggctcgt tcgacacgct taccgattat gcgatctttg ctttgacgtt
     2761 gttctatgcg ctggttgccg cgtcggtctt tatctttcgc cggaaaatgc ccgaggcgga
     2821 gcgaacctat cgcgtctggg gctatccctt cgtaccgatc atctttctaa ccgtgtcggc
     2881 atggcttatc gtcacgacga tttacaacac gccgcgacaa tccgcgatcg ggttggggct
     2941 gatcatgttg ggtgtgccgg tctattacct gttggatcgt cgccgctcga cttgaatcag
     3001 atccggttct tttccttcac tggtagcaag cagcgcgttg ccgtcgagcc ggtatgcaat
     3061 cgcctcgcct tgcttgcgtt tgcccaaat
//
LOCUS       DASTEY010000572         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1034663, whole genome shotgun sequence.
ACCESSION   DASTEY010000572 DASTEY010000000
VERSION     DASTEY010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034663"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VFO72_10065"
     CDS             <1..1264
                     /locus_tag="VFO72_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9479681.1"
                     /translation="DISLSRAELHRLVEEHFREVSASLPYFKRVKVMHFTDIELPRTA
                     TRKVKRREVVGIMESLEQKQKSGQQSLAQESADADSVWLMGIVASVSNRPRSEISIGT
                     RLSDLGFDSVMFVELATAIENAGGSISAPERFNEIQDVRELLSVVNRRPGASTKREIR
                     ADEGKKDDEIHIPAFVRTAGTKAGDFLQRFLYDQFLRTHYEGRSNIPPHTNFIVAANH
                     CSHLDMGLTKMALGDYGKDMVALAAADYFFDTKYKRAVMENFTNLVPMERTGSLRQSL
                     RHARSFLDQGYNALIFPEGTRSMTGVMADFKPVVGYLALHARVGILPMYLDGTYEAMP
                     KGTTIIKNRDVGARIGRFLPHEELEEMTRGLARAEAYRLIAARVRHEVVNLRDRTRAP
                     FDWQGLRRQWRNERKMHLIEEPEESLAV"
     gene            1264..>2029
                     /locus_tag="VFO72_10070"
     CDS             1264..>2029
                     /locus_tag="VFO72_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9479682.1"
                     /translation="MPTKSPGKRTARMTPVVSGTKRRAAIEKRQRATTLITGGTGFLG
                     AHLVRQLIDEGAKNIRVMATSIPDWLVDLGVETFAGSITNADDNKRAVEGISEIYHLA
                     GKVSRERKDSREMFDIHVEGTRLLCEAAKAAGTKTIILASTSGTIAVTKDADVVPDET
                     YPPPLEIISRWPYYASKAYQEMAALERFSGKGLRLVIMNPSLLLGPGDDRLSSTKVVL
                     DFMARKIGAVPTGGVSFVDARDAAKTFRTAMKKGRHG"
BASE COUNT          448 a          540 c          632 g          409 t
ORIGIN      
        1 cgacatttca ctctcgcggg cagaactgca ccggttggtc gaagagcatt tccgcgaagt
       61 ttctgcttcc ctgccctatt tcaaacgcgt caaggtaatg catttcacgg acatcgaatt
      121 gccgcgcacc gcgacgcgaa aagtgaagcg gcgagaggtg gtcgggatca tggaatcgct
      181 cgaacagaaa cagaagtcag ggcaacagtc tttggcgcag gagagtgcgg atgctgattc
      241 ggtgtggctg atgggaattg tggcgagtgt atcgaaccgg ccgcgttcgg agatatccat
      301 aggcacgcgt ttaagtgatc tcgggtttga cagtgtgatg tttgtcgagc tggcgactgc
      361 catcgagaac gcaggtggat cgatctccgc gccggaacgt ttcaacgaga ttcaggacgt
      421 gcgcgagctt ttgagtgtgg tgaaccgccg tccgggcgcg tcaacgaaac gagagatccg
      481 ggccgacgag ggcaagaaag atgacgagat tcacattccc gcgtttgtca gaacggcggg
      541 gacgaaagcc ggcgactttc tccagcgttt tctttacgac cagtttttgc gcacgcacta
      601 cgaaggccgt tcgaacattc cgccgcacac caacttcatc gtcgcagcga atcactgttc
      661 gcaccttgat atgggtctca caaagatggc gctcggcgac tacggcaaag acatggtggc
      721 gctggccgcc gctgattatt tcttcgacac gaagtacaag cgcgcggtga tggagaactt
      781 tacgaacctt gttccgatgg agcgcacggg atcgttgcgt cagtcgctac gacatgcgcg
      841 atcgtttttg gaccagggtt acaacgcgct catctttccc gaaggcacgc gatcgatgac
      901 cggagtgatg gccgatttca aacccgtcgt cggttatctg gcgcttcacg cgcgcgtcgg
      961 cattctgccg atgtatctcg acggcaccta cgaagcgatg cccaaaggaa ctacgatcat
     1021 caagaatcgc gatgttgggg cacgcatcgg gcgcttcctt ccgcatgaag aattggaaga
     1081 gatgacgcgc gggctggcac gcgccgaggc gtaccggctg attgccgcgc gcgtgagaca
     1141 tgaagtagtg aacctgcgtg accgcacgcg ggcgccgttt gattggcagg gactgcgcag
     1201 acagtggcgc aacgaacgca agatgcactt gatcgaagaa cctgaagaaa gcctggcagt
     1261 ttaatgccta ctaaatctcc aggtaaacgc actgcgcgca tgaccccggt cgtgtccggc
     1321 acgaagcgcc gcgcggcaat cgaaaaacga cagcgggcga ccacgttgat taccggtggc
     1381 accggttttc tcggcgcgca tcttgtccgg caactcatcg atgagggcgc gaaaaacatt
     1441 cgcgtcatgg cgacctcgat tccggattgg ctcgtggacc ttggcgtcga gacttttgcg
     1501 ggttcgatta ctaacgcgga tgataataaa cgcgcggtcg aaggcatcag cgagatctat
     1561 cacctcgcgg gaaaggtgtc gcgcgaacga aaagactcgc gcgagatgtt tgacattcac
     1621 gtcgaaggca cgcggctgct ctgcgaggcg gccaaagccg ccgggactaa gacgattatt
     1681 ttggcgtcga ccagcggcac tattgccgta acgaaagacg ccgacgtggt tcccgatgaa
     1741 acgtacccgc cgccgctgga gattatttct cgctggcctt actacgcgag caaagcctac
     1801 caggagatgg cggcgctgga acgtttcagc ggcaagggac tgcggctggt gataatgaat
     1861 ccgagtctgt tgcttggtcc gggcgacgat cgtttgagtt ccacgaaagt cgtgttggat
     1921 ttcatggcgc gcaagattgg agccgtgccg accggaggcg tcagttttgt cgacgcccga
     1981 gacgcggcga agacgtttcg cacggccatg aagaagggcc ggcatggcg
//
LOCUS       DASTEY010000573         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1479849, whole genome shotgun sequence.
ACCESSION   DASTEY010000573 DASTEY010000000
VERSION     DASTEY010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479849"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2574
                     /locus_tag="VFO72_10075"
     CDS             133..2574
                     /locus_tag="VFO72_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479683.1"
                     /translation="MGKDIRYAVRNLLKRPGFVAVAVITLALGIGANTAIFSLFNAVL
                     LRSFPVERAGEIVSVAMRGKDDSMSAFSYPNYRDFRDRNDVLAGLLVYRFVPLSLSRG
                     GNNERVWGYEVSGNYFDVLGVNAIKGRTFLPEEDKTPLSHPVAVLSYDSWQKRFGGDP
                     DLVGKDVLLNNHQFKVIGIAPAGFKGTELLYTPEIWLPAAMMEWAEPGATWLEDRDTR
                     NFFANGRLKPGVNARQAEASLNLLALQLAKEYPDSNEGQSVKVTPPGFLIPELRGAVV
                     SFTWVLMGAVALVLIVTCTNLAGLMLARATDRRREIAIRLAMGANRLRLIRQLLTESV
                     LLSFVGGLAGVLLAVWIIDLLLAFKPPIDYPLTLDVAIDWRVLVFSLAVSLLAGTIFG
                     LAPALQATRAGLSQALRETSAQGGAVRTRLRSLLVVAQIAISLVVLIAAGLVVRTLQQ
                     LQTMNPGFDPQNALTMSFDLGLQGYDEPRGQQFYRQLYERVQALPGVKSATISTFIPL
                     SLNYNSRNIFVEGQPAARGENVPLAMNASAGPRYFETMRTPLVQGREFTDHDQTKTEA
                     VAVVNETFVRRLLPHVSSSNEAIGKRISFNSPAGPFISIVGIAGDGKYFNITEDPRPF
                     IWTPLTQDYWSNGILTVRTEGNPESLFAAVRKEIQALDPNLPLFDVKTLTDHMRLALF
                     PARIAATVLGVFGLVALLLSAIGIYGITSYTVAQRTHEIGIRLALGAQLSNVLKLVLS
                     HGLKLTIIGAALGVFGAYVATRAITSVLYGVSATDPFTFVFVSVLLVGVALVASYVPS
                     RRATKVDPLVALRNE"
     gene            2603..>2812
                     /locus_tag="VFO72_10080"
     CDS             2603..>2812
                     /locus_tag="VFO72_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479684.1"
                     /translation="MTKWFLSALALVLLFSLAVTAQKDADQDREAVRQAVLDYVEGIY
                     NVEPSRIERSVSPNLAKIGFYRPPAE"
BASE COUNT          567 a          762 c          832 g          651 t
ORIGIN      
        1 cgttgaaacg gtttcctccg tcttcactgc tcctgcaccg ggctaaagcc cggtgagaat
       61 gagagaggac ttttgaggca aagcagtccc aaataaacag attcttctac tgtagctgta
      121 ctaataaaaa ctatgggaaa agatattcgc tacgctgtaa gaaacttgct caagcgtccg
      181 gggttcgttg ctgttgcggt gatcacgctg gcgctgggca ttggtgccaa taccgcgatt
      241 ttcagtcttt tcaatgcagt gctgttgcgt tccttcccgg tcgaacgtgc cggtgagatt
      301 gtctcagttg ccatgcgcgg caaggacgat tcgatgtcgg cgttctcgta tccaaactac
      361 cgcgactttc gcgatcgcaa cgacgtgctt gcgggactgc tggtctatcg tttcgtgccc
      421 ctgagcctga gtcgtggcgg taataacgaa cgcgtttggg gttatgaagt ttccggcaat
      481 tacttcgacg tgctcggcgt gaacgcgatt aagggtcgca cctttcttcc ggaagaagac
      541 aagacgccgc tgtcgcatcc agtggctgtt cttagttacg actcgtggca aaaacgcttt
      601 ggcggcgatc ccgatctcgt cggcaaagac gtgctgctga ataatcacca gttcaaagtg
      661 atcggcatcg cgcccgcagg tttcaagggt actgaactac tgtacacacc ggagatctgg
      721 ttgccggccg cgatgatgga atgggccgaa ccaggcgcaa cttggctcga agatcgtgac
      781 acgagaaatt tcttcgcaaa cggccggctg aaaccgggcg tcaacgcccg ccaggcggaa
      841 gcgtcgctta acctcctcgc actacagctc gcgaaagagt atcccgactc gaacgaagga
      901 caatcggtca aggttacgcc gccaggtttt ctcattcccg aattgcgcgg cgccgttgtc
      961 agctttacgt gggtattgat gggcgcggtg gcgctggtcc tgatcgtcac gtgcacgaac
     1021 cttgccggat tgatgctcgc gcgcgcgacc gatcggcggc gcgagatcgc gattcgtctg
     1081 gccatgggcg cgaatcgttt gcgtttgatc cgtcaactgc tcaccgaaag tgtgttgctc
     1141 tcattcgtag gcggtctcgc cggcgtgtta ctggcggtct ggattatcga cctgctgttg
     1201 gctttcaaac ctccgatcga ttacccgttg acgctggatg tggcaatcga ttggcgcgtg
     1261 ttggtgtttt cactcgcggt ttcgctgttg gcgggaacga tcttcggcct ggcacccgcg
     1321 ctccaggcga ctcgggccgg tctttcgcaa gccttaagag aaacgtcagc acaggggggc
     1381 gcggtaagaa ctcgtttgcg cagcttgctg gtggtggcgc agatcgcaat ctcgttggtc
     1441 gtcttgatcg ctgcgggact ggtagtgcgc acgctccagc aactgcaaac gatgaatccc
     1501 ggattcgatc cacagaacgc attgacgatg tcgttcgatc tgggcctgca aggctatgac
     1561 gaaccacgcg gacagcagtt ctatcgtcaa ctatacgaac gcgtacaggc gttgccgggt
     1621 gtgaagtcgg ccaccatctc gactttcatt ccgctgagtc tcaactacaa cagtcgaaac
     1681 atcttcgtcg aaggtcaacc ggccgcacgc ggtgagaacg tgccgctggc gatgaacgcg
     1741 agtgccggac cgcgctactt cgaaacgatg cgcacgccgc tggtccaggg gcgtgaattc
     1801 accgatcacg atcagacgaa gactgaggca gtcgcggtgg tgaatgaaac ttttgtgcgg
     1861 cggttgctgc cgcacgtgag ttcgagcaat gaggcaatcg ggaagcgtat ctcatttaac
     1921 agcccggctg gtccattcat cagcattgtc ggcattgcgg gcgatggcaa gtatttcaac
     1981 atcactgaag atccgcgccc gtttatctgg acaccgctga ctcaggacta ctggtctaat
     2041 gggattctaa ccgtgcgtac ggaaggcaat ccggagtcgt tgttcgccgc agtgcgcaaa
     2101 gagatccagg cgctcgatcc aaacctgccg ctcttcgacg tcaagacatt gaccgaccac
     2161 atgcgactcg cgcttttccc cgcgcggatc gccgcgactg ttcttggagt cttcgggctg
     2221 gtcgctttgt tgctgtcggc gatcggcatt tacggcatta cgtcttatac ggtggcgcag
     2281 cgcacgcacg agatcggaat cagactcgca ctcggcgcgc agttgtcgaa cgtgttgaag
     2341 ctcgtgctga gtcatggatt gaaactcacg atcatcggcg ccgcgctcgg agtgtttggt
     2401 gcgtatgtcg cgacgcgcgc gatcacgagc gtgctgtacg gcgtgagcgc aaccgatccg
     2461 ttcacgtttg tgttcgtgtc ggtcctgctc gttggcgtcg cgctggttgc ttcttacgtg
     2521 ccttcacggc gggccacgaa agttgatccg ctggtcgcgc tgagaaacga ataaggacca
     2581 attgcaagaa ggagattgaa agatgacgaa atggttttta tcggcactgg cgctggtgct
     2641 gttgttttca ctcgcggtta cagctcaaaa agatgcagac caagatcgag aagccgtccg
     2701 ccaggcagtg cttgattacg tcgaaggtat ctacaacgtc gagccgtcgc gaatcgaacg
     2761 cagtgtcagt cccaacctcg ccaagattgg gttctatcgt ccgccggcgg ag
//
LOCUS       DASTEY010000574         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_342607, whole genome shotgun sequence.
ACCESSION   DASTEY010000574 DASTEY010000000
VERSION     DASTEY010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342607"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFO72_10085"
     CDS             <1..346
                     /locus_tag="VFO72_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479685.1"
                     /translation="RLASVGWPFSIGVPILVAAPALFLWFAETYTIDLLAGKLPGTPP
                     AVAARTVSTLLFCALGSALLVQPATLICAIISLFIPTRSRTDAFSSGRAFAWAITGTL
                     LFVFAVALFVVV"
     gene            complement(577..777)
                     /locus_tag="VFO72_10090"
     CDS             complement(577..777)
                     /locus_tag="VFO72_10090"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET9479686.1"
                     /translation="MSATATPQKRKSIDCREFPSEKNCSVKISGSEDEVIDLAVLHAT
                     SFHGHENNPELRDEIRNLLKDE"
     gene            934..1086
                     /locus_tag="VFO72_10095"
     CDS             934..1086
                     /locus_tag="VFO72_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479687.1"
                     /translation="MGKSTQFDRDDDADVKKVVSKTAPLGYLPILYVGVSLLFIIVVL
                     FLWWRS"
     gene            1160..2941
                     /locus_tag="VFO72_10100"
     CDS             1160..2941
                     /locus_tag="VFO72_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479688.1"
                     /translation="MRVLRILFSFLLLFAIVSLPVQTLAQNPSADVVMVLPFENTSNR
                     AEYNWVGESFADALAELLNKPGLVVVTSAERELAYQQLRLPETVLPSRATAIKLARQA
                     KATMIVIGSYSVTPPATPAASEPKNQEKLPADAYVQVTARAIKVNEGRTMGEVLDGGW
                     ATRQFDYGGPLTTLQDIHGRLAYQILYQRDKALPYSQNQIVQEATKIPQKAFEAYVKA
                     VQLNLRDEVRARYLKNALHYYAEALGGAVYPQAAFELGRGYMSLGNWKEATEYFSKVQ
                     KKEPHYAEAAFYASLGYAKLGDYGRALATVVPLSSDLPLIGVYNNAGAVAVQAARQNK
                     NDGERTRLLKQASEFLARAAESAPNDQMVHFNYGYALFLSGKYGEAAEHFRPVITADQ
                     RDGQAYFLFAKSLAKTGKIEAAAAADDQARRYLQSAYAKWETEWQKSQTTKNVTLRMR
                     DVLNREEVFSLVREKNITAEANSPSSATQDLLVKARDLYQAGRDDEALPELHRVVMIE
                     PTNAEAYLLSGRINLRRSDQEAAIAALKTAIFWDPKLIDAHILLGRIFLERGDRGEAR
                     KYAFSAITIDPNNQEAIALQRQVTMGN"
     gene            2942..>3122
                     /locus_tag="VFO72_10105"
     CDS             2942..>3122
                     /locus_tag="VFO72_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479689.1"
                     /translation="MSYFRLLVAFAKSRSRKRRPRLPAVSSPAIVNTDLNPRGSVLVG
                     GELWTAETTRGDFIPR"
BASE COUNT          673 a          891 c          868 g          690 t
ORIGIN      
        1 cagactggct tccgtcggtt ggcccttttc catcggtgtt ccaattctgg tggccgcgcc
       61 ggcgttgttt ctctggtttg cggagacgta cacgatcgat ctgcttgcgg gtaaactccc
      121 cggtactccc cctgcggttg ccgcacggac cgtaagcact cttctcttct gcgcgcttgg
      181 ttcggctctg ctggtccaac ccgcgacctt gatctgcgca atcatctccc tcttcatacc
      241 cacccgatcg cggacggacg cattctcctc aggccgggca ttcgcttggg ccatcacggg
      301 aacgctgctg tttgttttcg ctgtcgcctt gttcgtcgtc gtttgagtca gttgtaaagg
      361 actacgacgc gaacggtgcc gcttccattg ggggaaaaag cgtcctgctt ctgcggatat
      421 aagcggcggc ttggtgagtt tgaacaccta cctgctggga ccgcaggcgt ctcgcctgcg
      481 tgtttcgaaa gcagctctct cggctgccat gcaggcgaga cgcctacggt cccaggcaca
      541 actgcatcac tattcttccg gctctgccgc cgcggtttac tcatccttaa gtaagttgcg
      601 gatctcgtct ctcaactcag ggttgttctc gtggccgtgg aaagacgtcg cgtgtagcac
      661 agcgaggtcg ataacttcgt cttcactgcc cgatatcttc accgaacaat tcttctcgct
      721 cggaaactct cgacagtcga tagacttgcg cttttgtgga gttgcggttg ctgacatgtg
      781 tgttgactcc tttttttgag atgacctgga ttctagcgca aaacagatgg gcagttaaac
      841 gtccccgaag aagcggcatg gctcttgtaa aggttattaa taaacctgtg tccccggccg
      901 gttacggtac agaaacgaac caggagtgtt cacatgggaa agtccactca attcgatcga
      961 gacgacgatg ctgacgtgaa gaaagtcgta tcaaaaactg cgcccctcgg gtacttgccg
     1021 atactctacg tgggcgtctc gctcctattt atcatcgtag tgctcttcct gtggtggcgc
     1081 agttaagatc gagtaggcca cagatctgac ggattacgca tctgtgtaaa ctgtggccat
     1141 ttctgctaca gtaccgacca tgcgagtttt aagaatactt ttcagctttt tactgctttt
     1201 tgccatagtt tccctgccgg tccaaacgct ggctcagaac ccgtccgcgg acgtcgtgat
     1261 ggtgttaccg ttcgaaaaca cctccaaccg cgccgaatac aattgggtcg gcgagagttt
     1321 cgccgacgcg ctcgcggaac tcctgaacaa gcccggtctg gtcgtcgtta ccagcgccga
     1381 acgcgaactc gcttatcagc agttgcgctt gcctgaaacc gtacttccct cgcgagccac
     1441 ggcgatcaaa ctcgcgcggc aagccaaagc cacgatgatc gtgatcggca gttactcggt
     1501 tacgccacct gcgacgcccg cggcgagtga accgaagaac caggagaaac tacccgctga
     1561 cgcttacgta caggtcaccg cgcgcgcgat caaagtgaac gaaggacgca cgatgggcga
     1621 ggtgctggac ggcggatggg ccacgcggca gtttgattac ggtggtccgc tcaccacgct
     1681 tcaggacatt cacggccggc tcgcgtacca gattctttac cagcgcgaca aagctctgcc
     1741 ctactcacag aaccagatag ttcaggaagc cacgaagatt ccgcagaaag ctttcgaagc
     1801 gtatgtaaaa gcggtgcagt tgaacctgcg cgacgaagtg cgtgcgaggt acctcaagaa
     1861 cgcgctgcac tactacgcgg aagcgctcgg tggcgcggtg tatccgcagg ccgcgtttga
     1921 gctcggtcgc ggttacatga gtctcggcaa ttggaaagaa gccacggaat atttttctaa
     1981 agtgcagaaa aaggagccgc actacgcaga ggcagcgttc tacgcgagtc ttggctacgc
     2041 aaagctcggc gattacgggc gtgcattggc gacagtagtg ccgctctcca gtgatctgcc
     2101 gctgatcggt gtctacaaca atgccggcgc cgttgccgtg caggctgcgc gccaaaacaa
     2161 aaacgacggt gaacgaacac gtctgctaaa acaggcctcg gagtttctcg ctcgcgcagc
     2221 tgaatcggcg ccgaacgatc agatggtcca cttcaattac ggttacgcac tctttctgag
     2281 cggaaaatac ggagaagctg ccgaacactt tcgtcccgtg atcactgcgg atcaacgcga
     2341 tggtcaggcg tatttcctgt ttgcgaagtc gttggcaaag accggaaaga ttgaagcggc
     2401 tgctgccgct gacgatcagg cgcgacgcta tctgcaaagc gcttacgcga agtgggaaac
     2461 cgagtggcag aaatctcaaa caaccaaaaa cgtcacactg cggatgcgcg acgtgctgaa
     2521 tcgtgaagaa gtattcagcc tggtgcgcga gaagaacatc accgccgagg ctaattcgcc
     2581 aagctcggcg acgcaggact tgctggtcaa ggcgcgcgat ctttatcagg ctggacgcga
     2641 cgacgaagcg ctgccggaac tccatcgagt ggtaatgatc gagccgacga acgccgaggc
     2701 gtatttgctg agtggccgta tcaacttgcg ccgcagcgat caggaagccg cgatcgcggc
     2761 tttgaagacc gctatcttct gggatccaaa actgattgac gctcacattc tcctgggcag
     2821 gatctttctc gaacgcggcg accgcggcga agcgcgaaag tacgccttca gcgcaatcac
     2881 aatcgatcca aacaaccagg aagcgatcgc gttgcagcgt caagtgacga tgggtaatta
     2941 aatgtcgtac tttcgactgc tcgtcgcctt cgcaaagtca cgaagcagga aacggcgccc
     3001 tcgtcttccc gctgtttcct cacctgcgat tgtcaacacc gacctcaatc cgcgcggttc
     3061 agtactcgtc ggtggcgagt tgtggactgc ggagaccacg cgtggcgact ttattccgcg
     3121 cc
//
LOCUS       DASTEY010000575         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_721965, whole genome shotgun sequence.
ACCESSION   DASTEY010000575 DASTEY010000000
VERSION     DASTEY010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721965"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFO72_10110"
     CDS             complement(<1..1100)
                     /locus_tag="VFO72_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9479690.1"
                     /translation="MIEALNLVLVFLLVLANGFFVASEFALVGVRKSRIETLAKAGDI
                     RARRLLPLLNNLNAYISATQLGITLASLALGWIGEPAVAHLLEVPLKGRVSETVLHTI
                     AFTIAFSIITFLHIVLGELAPKTLALERAERTALAIALPLQIFYRVFRWPIQLLDWAG
                     TRTVRLFGLHPSSGHASIYTEDELRQLIDISHQSGHLQADEQRLINRIFDFSDAQVRE
                     AMIPRTSIAALPVTATLQETKEALRSLGYSRLPVYQDVLDNVVGVLVRRDFEPYLDQT
                     TAPMKFNLRELIHTPHFIPATARLDTALKQMQSKRTHLAFVVDEHGGIEGIVTLEDLL
                     EEIVGEINDEFDDEVREQITKEPDGSYSLSGIL"
     gene            complement(1173..1535)
                     /locus_tag="VFO72_10115"
     CDS             complement(1173..1535)
                     /locus_tag="VFO72_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479691.1"
                     /translation="MRQSSFAIHMSLMESQYFAATAIHAGALRGAEEMSLLKLPKRKI
                     PISIQVGDSDQFFSLKVVRETRDALKDAGFPVDLIEIKNHNHWYYDSASKFNQTAWEF
                     LNQHELDADPQYQSYTWN"
     gene            complement(1636..2118)
                     /locus_tag="VFO72_10120"
     CDS             complement(1636..2118)
                     /locus_tag="VFO72_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479692.1"
                     /translation="MRILLTLLVLFLASGHAVAKDDITKELTTSNGKKRVYYLYVPST
                     LKPDTQAPLLVTLHGSGRNGITLVEKWKDFAKKEGIILAGPDASQPAGWSAPQDGPDF
                     LRDLVEELKTRYAINPRRVYLFGHSAGACFARLCLKSLLSFSPGFSPVLPPRGFEKPF
                     "
     gene            complement(2150..>2791)
                     /locus_tag="VFO72_10125"
     CDS             complement(2150..>2791)
                     /locus_tag="VFO72_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479693.1"
                     /translation="EERSTRRDFVLEPVANAATPGEALLLLQNSGRSRYRELQAVARF
                     RFQEGRNAYLSYVRSQARGNLNDFNTYFGNQKHAVIRPDEYGKQPYDVPHRLLFWGDF
                     GLPFNVVATPVLDWRSGFPFSVLDEQQDFAGPRNEGGRFPQLVTLDLLVTKGLTIPFR
                     GKKYKGRAGMTIFNITNHWNPRDVQNNVASPQFGTFFNSPDRSVRLKFEFVKY"
BASE COUNT          616 a          731 c          799 g          645 t
ORIGIN      
        1 aggatgccgc tcaatgaata gctgccgtca ggttccttcg ttatctgttc gcgtacctca
       61 tcgtcaaatt cgtcgttgat ctcgccgacg atctcttcga gcaggtcttc cagcgtcacg
      121 atgccctcaa tgccgccgtg ctcgtccacg acgaaggcta gatgagttcg tttcgactgc
      181 atctgtttga gcgccgtgtc taaacgcgcc gtggcgggaa tgaaatgcgg tgtgtgaatt
      241 agttctcgca gattgaactt cattggcgcg gtcgtttggt ccagataagg ctcgaagtca
      301 cgtcgcacga gaacaccgac tacgttgtcg agaacgtcct ggtacaccgg caaacgggaa
      361 tatcccagac tgcgaagcgc ttctttcgtt tcctgcaacg ttgcggtcac tggcagcgcg
      421 gcgatggacg tgcgcgggat cattgcttca cgcacctgcg cgtcagaaaa atcaaagatg
      481 cgattgatca ggcgttgttc gtcggcttgc aggtgcccac tctgatgcga gatgtcgatc
      541 agttggcgta gctcgtcttc agtgtagatc gacgcgtgtc cgcttgacgg gtgcaggccg
      601 aacaggcgca cggtccgcgt tccggcccag tcgagcagtt gtatgggcca gcgaaacacg
      661 cgatagaaaa tctggagtgg caaagcgatt gccagtgctg ttcgttccgc ccgttcgagg
      721 gcgagagtct ttggcgccaa ctcaccgagc acgatgtgca gaaacgtgat gatactgaac
      781 gcgatagtga acgcgattgt gtggagaacg gtctcggaga cgcgaccctt gaggggaact
      841 tcgagcaggt gcgcaactgc cggctcgcca atccagccga gcgccagtga cgccagagtg
      901 attccgagct gggttgctga gatgtaagca ttcagattgt tgagcaacgg cagcagccgt
      961 ctggctctga tatcgccggc tttcgcgagc gtttcaattc gtgacttgcg aacgccgacg
     1021 agcgcaaatt ccgaggccac gaagaagccg ttcgccagca ccagtagaaa tacgagtacg
     1081 agattcagcg cttcaatcat gctagtagga cagacattct tgtctgtctt caaattacca
     1141 accgagacag gcaggaatgc ctgtcctacg cactagttcc aagtgtaact ttgatactgc
     1201 ggatctgcgt ccagttcgtg ttgattcaga aattcccagg cagtttgatt gaactttgac
     1261 gccgagtcgt aataccagtg attgtgattc ttgatctcga tcaggtcgac gggaaaaccg
     1321 gcgtccttca acgcgtctct cgtctcgcgc acgaccttga gcgaaaagaa ctgatcgctg
     1381 tcgcctacct gaattgagat gggtatcttc cgtttcggca gcttgagtaa cgacatctct
     1441 tcggctcctc tgagtgcgcc ggcgtggatc gcggtagcgg cgaagtattg cgattccatc
     1501 aacgacatgt gtattgcaaa actgctttgc ctcaaaagtc ctctctcatt ctcaccgggc
     1561 ttcagcccgg tgcaggagca gtgaagacgg aggaaaccgt ttcaacggtt ttccgttgat
     1621 tcgccagcga aaccgttaaa acggtttctc aaaaccgcgt ggcggtaaca ccgggctaaa
     1681 gcccggtgag aatgaaagaa gacttttgag gcaaagcctc gcaaaacaag ctccggccga
     1741 gtggccaaac agatagacgc gacgtggatt gatcgcgtac ctggttttga gttcttcgac
     1801 gagatcgcgc agaaagtcgg gtccatcctg tggcgccgac cagccggccg gctggctggc
     1861 gtcaggtccg gcgagaatga ttccttcctt tttcgcgaag tctttccatt tttcgacgag
     1921 cgtgatgccg tttcttccgg agccgtgaag cgtcacgagc agaggcgctt gcgtatccgg
     1981 tttgagggtt gacggtacgt acagatagta gacgcgcttt ttaccgtttg aagtggtcaa
     2041 ctctttggtg atgtcgtctt ttgcgacagc atgaccagat gccagaaaca gtacgagaag
     2101 agttaagagg attctcattt ggcaactcca gggaacaggg atttgacgat caatacttca
     2161 caaactcgaa tttcaaccgc acgcttcgat ccggactgtt gaagaaagtt ccgaactgcg
     2221 ggcttgcgac attattctgc acgtcgcgcg ggttccagtg attcgtgatg ttgaagatgg
     2281 tcatgcccgc gcgccctttg tatttcttgc cgcgaaaggg aatcgtaagc cctttggtca
     2341 cgagcagatc gagcgtgacc aactgcggaa agcggccgcc ctcgtttcgc gggccggcga
     2401 aatcctgctg ttcgtcgagc actgaaaacg gaaacccgct gcgccaatcc aacaccggtg
     2461 tggcgacgac attaaagggt agaccgaagt cgccccagaa gagcagccgg tgcggcacat
     2521 cgtagggttg tttaccgtat tcgtccggac gaatcaccgc gtgcttctga tttccgaagt
     2581 aggtgttgaa gtcgttcaag ttgccgcgag cctgcgatcg cacgtacgac aggtaagcgt
     2641 tgcggccttc ctgaaaacga aatcgcgcga cagcttgcaa ttcgcgataa cgagatcttc
     2701 ctgagttttg caataaaagc aaggcttctc cgggcgtagc ggcattggcg accggctcga
     2761 ggacgaagtc acgacgcgtg ctgcgctctt c
//
LOCUS       DASTEY010000576         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1331497, whole genome shotgun sequence.
ACCESSION   DASTEY010000576 DASTEY010000000
VERSION     DASTEY010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331497"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFO72_10130"
     CDS             complement(<1..634)
                     /locus_tag="VFO72_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9479694.1"
                     /translation="MLRVTEIFRSIQGESTHAGRPCTFVRLTGCPMRCVWCDSEYTFT
                     GGEHVAIDDVMRQVHSLGCKLVEVTGGEPLAQKEAFELIRRLCDEDFEVLIETGNYAS
                     TAGLDPRAKVILDIKCPASGEESRNEWSNIERLREDRDEVKFVVANEDDWLYATRVIQ
                     DKDLETRAHAILISPVWDQIDLKQLAEWIASSGLNVRLQLQLHKYIWGPHV"
     gene            806..1732
                     /locus_tag="VFO72_10135"
     CDS             806..1732
                     /locus_tag="VFO72_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9479695.1"
                     /translation="MASRSDEIANLIKDGAGRLIARAADSRSLNVDALIPRVAAAVEK
                     YLLRYDANTSTAEINRFVDEMQAEDLCLIIACERGDEKAWTDLVERFNTTVRSAARSA
                     SANEDAAEELAQSIWAELYGLRVRDDGRPASKLAYYSGRGSLAGWLRAVVAQLSVDAF
                     RKQSKLVQTEEDADLDRLARDARIGETQTFAAAGVQNPEQSLSNQFAQSDLQQALTQA
                     IRELAAEDRLLVKLYYFDNLRLREAGVVLGVHEATASRRLTRIQTDLRKRVSHILVAE
                     RGWTKAEAERSFAEVAQHLDTDLEALINEKSF"
     gene            1785..2969
                     /locus_tag="VFO72_10140"
     CDS             1785..2969
                     /locus_tag="VFO72_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479696.1"
                     /translation="MRRETNNDIDLLLRRLSRRDGISAADGEAQNADTHLDADELSSY
                     AQNALPATARARYTEHLAECSTCRKLVTELSLSLGATAASRSVETVLEPSGLKKFLAA
                     LFSPMVLRYAVPALGVIVVMVVGFVVLRRQQQSYELARVDVEKQQRGSVPAVPETPAP
                     TPASGAPLDGFVDAPEEKAGNTQRPAINADESRSASGTTAKTDAISPPARVASQRQEG
                     AAGAGAVAVASPSSPRAKTEIAEAEAKKDLEATAKRQAEPRRQTGDFQAQKESANEAA
                     RDRRTAPAAATGSVDQNKPKAAPSETQALSTARPASRGRVQSEKDESDTRSIAGRRFR
                     KERGIWVDTAYDSSTATVNVARGSEQFRALIADEPVIKAIAEQLDGEVIIVWKGRAYR
                     IR"
     gene            2988..>3442
                     /locus_tag="VFO72_10145"
     CDS             2988..>3442
                     /locus_tag="VFO72_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479697.1"
                     /translation="MNTTKLGTTLLCAVLSVTSFGAANSKPVQRKPRNPGIASIVRQI
                     DARNIERIIRQLVSFGTRNTLSEQNDPKRGIGAARDWLYAEFLKVAETSGGRMTVEKQ
                     SYEQPKAQRIPQPTIITNVVATLKGSQPQATDRIYVVSGHYDSMCNSPT"
BASE COUNT          773 a          986 c         1003 g          680 t
ORIGIN      
        1 gtacgtgagg tccccagatg tacttatgca gttgtaattg cagccgcaca ttcagaccac
       61 ttgacgcgat ccattctgcg agctgtttca gatcgatttg gtcccacaca ggcgagatca
      121 ggatggcgtg cgctcgcgtc tcgagatctt tatcctgaat cacgcgcgtt gcatacagcc
      181 aatcatcttc attcgcgacg acaaacttca cctcgtcgcg atcctcgcgg agccgctcga
      241 tgttggacca ctcgtttctc gactcttcgc cggatgcggg acacttgata tcgagaatga
      301 ctttcgcgcg cgggtcgaga cccgctgtag acgcgtagtt tcccgtttcg attagcactt
      361 cgaaatcttc gtcacagagc cgccgaatca gttcgaacgc ctctttttgg gcgagtggct
      421 cgccaccagt tacttcgacc agcttgcaac ccagtgagtg gacttgccgc atcacatcgt
      481 cgatggcgac atgttcgccg ccagtgaagg tgtactcgct gtcacaccag acgcagcgca
      541 tcggacagcc ggtcaggcgc acgaaggtgc acggtcgccc ggcgtgggtc gattcgcctt
      601 gaatggagcg aaagatttcc gtaacgcgca gcatttaacc ggaatcttac acggttctac
      661 acaaatcaag aaccgtcttt cctgtgaccg caggcgtctc gcctgctaac cttcgctacg
      721 tgtccggttc gagcaaggca ggcgcgacgc ctgcggtgac agggaagagg gtgctaggat
      781 tacgaggagc tgtttagagt ccccggtggc aagcagaagc gacgaaattg ccaatctaat
      841 aaaggacggc gccggccgat taatcgcacg cgccgcggac agccgttcgc tcaacgtcga
      901 cgcgttgatc ccacgcgtcg cagcggcagt cgaaaagtat ctcctgcgct acgacgcaaa
      961 cacctccacg gctgaaatca acaggtttgt cgatgaaatg caggctgaag atctgtgcct
     1021 gattatcgct tgcgaacgtg gcgacgagaa ggcctggacc gacttggtcg aacgtttcaa
     1081 cacaaccgtc cgatccgcgg ctcgttctgc gagtgcaaac gaagacgccg ccgaggaact
     1141 ggcccaatcg atctgggccg aactttatgg actccgcgtt cgcgacgacg gccggcccgc
     1201 aagcaagctc gcgtactact ccggacgtgg ttcgctcgcc ggctggcttc gcgctgtcgt
     1261 cgcacagctt tcggtcgacg cgtttcgcaa acaatcaaaa ctggtgcaga cagaagagga
     1321 cgctgatctt gaccgcctcg cgcgcgacgc gcggatcggc gagacgcaga cgttcgccgc
     1381 cgctggagtt cagaatcccg agcagtcgct ttcaaatcag ttcgcacaga gcgacttgca
     1441 acaggcattg acgcaggcaa tccgtgagct cgctgccgaa gatcgcctgt tggtgaaact
     1501 ttattacttc gacaacttac gactgcgcga agcgggcgtt gttttgggcg tacacgaggc
     1561 gactgccagc cgtcgtttga cgaggattca gaccgatttg cgtaagcgcg tcagtcacat
     1621 attggttgct gaacgaggtt ggacaaaagc cgaggccgaa cgctcgtttg ccgaggtcgc
     1681 gcagcatttg gacacggatt tagaggcatt gatcaacgag aagagctttt gactgcaagg
     1741 tatggagatg gctacgttct ctctttggca gcgcttgatt gcaaatgaga cgagagacta
     1801 acaacgacat cgatctgttg cttcgccgcc tgagccggcg cgacggcatt tctgccgccg
     1861 acggtgaggc acagaatgcg gatacgcatc tcgatgctga cgagctgagt tcgtatgcgc
     1921 agaacgcgct gccggcgacg gcgcgtgcac gttacacgga acacctggcg gagtgttcga
     1981 cgtgcagaaa actggtaacc gagttgagcc tgtcgcttgg cgcaaccgct gcctcgcgtt
     2041 ccgtagaaac tgttttagag ccgagtggat tgaagaagtt tttggcggct ttgttctcgc
     2101 cgatggtttt gcgttacgcc gttccggcat tgggcgtgat cgtggtgatg gttgttgggt
     2161 tcgtggtgct gcgacgtcag caacagtcct atgagttggc gcgtgtggac gttgagaaac
     2221 aacaacgagg ttcggttcca gcggtgccag aaacgccggc gcccacgccg gcgtcaggtg
     2281 ctccgctcga cggatttgta gacgcgcccg aagaaaaagc aggaaacact cagaggcccg
     2341 ctattaacgc tgacgagtct cgtagcgcga gcgggacgac ggctaagacg gatgcgatct
     2401 ctccgcctgc gcgggttgcg tcccaacgcc aggaaggggc cgcgggagcc ggcgcagttg
     2461 cggtagcgag cccgtcttca cctcgggcga agactgagat cgctgaagcc gaagctaaaa
     2521 aggacttgga agcaaccgcc aagagacaag cagaaccgag gcgtcaaaca ggtgacttcc
     2581 aggctcaaaa agagagcgcg aatgaggccg cgcgtgaccg ccgcaccgct ccggcggcgg
     2641 caaccggtag tgttgaccaa aacaaaccga aggctgctcc gtccgaaaca caagcactct
     2701 ccactgcgcg tccggcctcg cgcggccggg ttcaatcaga gaaggacgaa tcagacacac
     2761 gctccatcgc gggccgccgc ttccgaaaag aacgtggcat ttgggtcgac acagcgtatg
     2821 attcctcgac cgcaactgtg aacgtggctc gtggctccga acagtttcga gcgctcatcg
     2881 ccgacgagcc cgttattaaa gcgatcgcag aacaactcga cggcgaggtc atcattgttt
     2941 ggaagggccg cgcttatcgc atccgttagg agccacagat tacacagatg aacacaacga
     3001 aacttgggac caccctcctc tgcgcagttc tgagtgttac ttctttcggt gcagcaaact
     3061 caaagccggt tcaacgaaag ccacggaacc ccggaatcgc cagcatcgtt cggcagattg
     3121 acgctcgcaa catcgaacgc attattcgcc agctcgtctc cttcggcacg cgcaatacgt
     3181 tgtccgaaca gaacgatccc aaacgcggca tcggcgcagc gcgcgattgg ttgtatgccg
     3241 agtttttgaa agtcgcggaa acttcgggcg gccgcatgac cgtcgaaaag cagagttacg
     3301 aacaaccgaa agcgcagcgt atcccgcagc cgacaatcat caccaacgtg gtagcaacgt
     3361 tgaaaggatc acaacctcaa gcgaccgatc gcatctacgt cgtcagtggt cactacgatt
     3421 cgatgtgcaa cagtccgacc ga
//
LOCUS       DASTEY010000577         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1315266, whole genome shotgun sequence.
ACCESSION   DASTEY010000577 DASTEY010000000
VERSION     DASTEY010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315266"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2524
                     /locus_tag="VFO72_10150"
     CDS             <1..2524
                     /locus_tag="VFO72_10150"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET9479698.1"
                     /translation="ARKYQGPYNELARSFNANEIYELYVRLSSTNNEPDEAGSDGPHE
                     GGVESLVVGLNREGRAIAAPVMQIDRWQAEFAGGRWVFANFTGPLTANAVRVLIERAA
                     QGAVRFRVRSDFACYRPGESSTLSVELTGRHSENVSIEVRSPSNRVIARDRQVGLVSE
                     AGLYQIKARIGELTAESGFWVYDSELLRSGKALTVDNHFFYRDGKVFPVTGATYMASD
                     VHRRFLFEPNPLVWDRDFREMKQAGVNMVRTGIWTGWKKYMPEPGKVNEGVLRAFDAF
                     LLTAHKYDIPVIFTFFAFLPETWGGSNAYLDPRAVRAQQQFISAFTERCRNVDDVLWD
                     FINEPSFCSPKYLWSCRPNYDEYEVAAWKQWLKDRYPAASDEDRVAQLQQLWRTTRDD
                     VLELPKLQDFDSVHLIDERQPLKTLDYRLFAQDMFIRWTRQMTTAVRTNGNRNQLITV
                     GQDEAGLGDSPNIQFFAREIDFTSLHNWWANDDLVWDSVLAKAPSKASLMEETGVMFY
                     ESADGDTPWRSEQEVSNLLERKMALSFAADGAGFIEWIWNTNPYMNSTNEVGIGFHRV
                     DGTAKPELEPFLTIAKFMSQHGHLLTDRQSEPVVMVIPHSHMFAPRSFAHEATRRCVR
                     AMYYHCGVPMQAVSEYTLPDYSEQAKLIVVPAPRALTQKCWDALMAQARGGATVAMSG
                     VINDEDWLPVAQTESFAVDGKEYLVRFEGEKIQRIEKSVTGISNGRPVITPHGVGRLI
                     RSPLPLELGDSMEALVAFYRLAMAHGRVAPIFTATPRTPAVLVLPSVFRNVVLYTFVS
                     EIDRDTKLQVTDLQTKTRFAVGVPAGRTAMTIIERRNGRRII"
BASE COUNT          592 a          684 c          800 g          569 t
ORIGIN      
        1 agcaaggaag taccaaggcc cttacaacga gctggcccgc tctttcaacg ccaacgaaat
       61 ttacgaactc tacgttcgtt tgagttcgac gaataatgaa ccggacgaag cgggcagtga
      121 tggaccacac gaaggtggag ttgagtctct cgtcgtcggg ttgaatcgcg aaggacgcgc
      181 gattgccgcg cccgttatgc aaattgatcg ttggcaggcg gagttcgccg gtgggcggtg
      241 ggtgtttgca aacttcaccg gacctctcac ggcgaatgcc gtgagagttt tgattgaacg
      301 agctgcgcaa ggtgcggttc gtttccgcgt gcgcagcgat ttcgcgtgtt atcgaccagg
      361 cgagtcttcg acgctctcgg tcgagttaac tggccgacac agcgagaacg tttccattga
      421 ggtacgcagt ccgtccaacc gggttattgc gagagacaga caagtgggat tggttagtga
      481 ggcggggctg tatcaaataa aagccaggat tggtgagctt acagcggaga gtgggttttg
      541 ggtgtatgac tcggaactct taaggagtgg gaaggctctg accgtcgata atcatttctt
      601 ctatcgcgac ggcaaggtgt ttcccgtgac cggcgcaact tacatggcgt ctgacgttca
      661 ccgccgcttt ctctttgaac caaatccgct cgtttgggac cgcgacttcc gcgagatgaa
      721 acaggccggc gtcaacatgg tccgcacagg catctggacc ggctggaaga aatacatgcc
      781 ggagcccggc aaggtaaacg aaggcgtgct gcgagcgttc gatgcttttc tgcttacggc
      841 ccacaagtac gacattccgg tcatcttcac gttcttcgct tttcttcccg agacgtgggg
      901 cggctcgaat gcgtatctcg atccacgcgc cgtaagagcg cagcagcagt tcatctccgc
      961 gtttactgaa cgctgtcgca acgtggacga tgtattgtgg gatttcatta acgaaccgtc
     1021 attttgttcg ccgaagtacc tctggagttg ccggccaaat tacgatgaat atgaagtcgc
     1081 ggcgtggaaa cagtggctga aggatcgcta tcccgccgcg agtgacgagg accgcgtggc
     1141 ccaactacaa caactctggc gcacgacacg cgacgacgtc ctcgaattgc cgaagcttca
     1201 ggatttcgac agcgttcacc tcatcgacga acgccagccg ctcaaaacgc tcgactaccg
     1261 gctcttcgcg caggacatgt tcattcgctg gacccggcaa atgaccacgg cggtgcgaac
     1321 caacggcaat cgaaatcagt tgatcacggt cgggcaggat gaagcaggat tgggcgacag
     1381 tccgaacata cagtttttcg cgcgggagat tgatttcact tcgcttcaca actggtgggc
     1441 caacgacgat ctcgtgtggg acagcgtgct ggcaaaagcg ccgtcgaaag cgagtctgat
     1501 ggaagagact ggcgtgatgt tttacgagag cgctgacggc gatacgccgt ggcgcagcga
     1561 acaggaagtc agcaatctgc tcgaacgcaa gatggcattg tcgtttgcgg ctgacggcgc
     1621 gggttttatc gagtggatct ggaatacgaa cccgtacatg aactcgacga atgaagtcgg
     1681 gattggtttt catcgcgttg atggaaccgc gaaaccggaa ctggagccgt ttctgacgat
     1741 cgcaaagttc atgagccaac acgggcacct gttaacggat cgacaatcgg aaccggtcgt
     1801 gatggtgatt ccgcattcgc acatgtttgc gccgcgaagt tttgcgcacg aagcaacgcg
     1861 tcgttgtgtg cgtgcgatgt attaccactg cggcgtgccg atgcaggccg tcagtgaata
     1921 cacgttgccc gattattcag agcaggcgaa gctgattgtt gtgcctgcgc cacgtgcgtt
     1981 gacacagaag tgttgggacg cgctgatggc gcaagctcgc ggtggtgcga cggtggcgat
     2041 gtcgggcgtg attaatgatg aagattggct gccggtggcg caaacggaga gtttcgctgt
     2101 tgacggtaaa gaatatttgg tcaggtttga aggagaaaaa attcaacgga ttgaaaaaag
     2161 cgtgaccggg atttcgaacg gaagaccggt cattaccccg catggtgttg gacgcttaat
     2221 ccggagtccg ttgccgctgg aacttggtga ttcgatggag gcgctggtgg cgttctaccg
     2281 gctggcaatg gcccacgggc gtgtggcgcc gatcttcacg gcaactccgc gcacgcccgc
     2341 ggtgctggtg ctgccgtcag tctttcgcaa cgtggtgctc tacacgtttg tatctgagat
     2401 cgatcgagac acgaagctac aggtgactga cctacaaact aaaacccgct tcgcggttgg
     2461 cgttcccgcc ggccgaaccg caatgacgat aatcgagagg cgtaacggca ggcggatcat
     2521 ttaacggcca cagaccaaca cagatttaac ggatcagaca cgaagtgggt gaacctgcgc
     2581 ccgaacccct gctagttcca ggtccaccca cttttgaaca caggcaccgc cccccaagct
     2641 cggcc
//
LOCUS       DASTEY010000578         6638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_540311, whole genome shotgun sequence.
ACCESSION   DASTEY010000578 DASTEY010000000
VERSION     DASTEY010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540311"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="VFO72_10155"
     CDS             <1..1669
                     /locus_tag="VFO72_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479699.1"
                     /translation="RKKDGTIINVEITANRVHFAGRSAEFVLAHDITERKQAEIALRI
                     SEDRYRDLVDNSHELICTHDLEGKVLSVNPWAARMLGYPQEALIGMNIRDGLLPEYRE
                     QFEEYLHTVKTEGSARGVMKVRTATGETRLWEYYNTLRTEGVEVPIVRGMAHDATERR
                     QALKREQEARLEAEAANRVKDEFLSTLSHELRTPLTAIMGWSDLLLHDEVAPRKRRQA
                     IETIARNANSQCQLINDLLEVSRIITGKLRLEFAACELESVIQAAVDSIRPTAEAKGV
                     RLQILLDPNAGPIFGDRERLQQVVWNLLSNGVKFTRAGGLVQVKLQRINSHVEVVVTD
                     TGVGINSDFLPHVFERFRQADGSTTRNYGGLGLGLAIVRHLVELHGGTAWAESGGHDE
                     GATFTVRFPAMIELEQRVEPPIETQTEFAVASRDRERTLRGLRVLVVDDEFDARVLVT
                     TMLERSGAQVLAVSSTREALESMQTWRPDVLIADIGMPVEDGYSLIRKVRALPSDRGG
                     QIPALALTAYARTEDRIRALSEGYQMHLAKPVDRVELATIIGSLCHR"
     gene            complement(1677..3239)
                     /gene="zwf"
                     /locus_tag="VFO72_10160"
     CDS             complement(1677..3239)
                     /gene="zwf"
                     /locus_tag="VFO72_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9479700.1"
                     /translation="MSTILVEPEPIHMEAPALPKAEPCVTVIFGATGDLTRRKLMPAL
                     WNLRNEGCLESVQILGVGRSPMTDDEYRAFVRKALLESEKLAPEEDAEWRKFAQRIFY
                     TTGELDDDNTYAKTAEKLEELGHKGASNNHLFYLATPPSLAPVIVEGLGKAGLAKQDH
                     DHWSRIVVEKPFGSDLESAQALNAKIAEVFSENQIYRIDHYLGKDTVQNILVFRFGNT
                     MFEPIWNRNYVDYVEITAAETAGVGSRAGYYEEAGALRDMVANHLLQLLTLTAMEPPV
                     AFDADSVREEKVQVLRSIRRLKPDEVAARTVRAQYGPGLIKGERVKGYREEEGVKNES
                     HTETYAAIEFRVSNWRWSGVPFYVRTGKGLGRTLTEIAVHLKRTPQALFARTPDDEIL
                     PNIIALRIQPNEGITVTFGAKRPGFEMRTTTVSMDFCYQRAFGVEPPAAYETLLLDVM
                     RGDAMLFTRGDEVEAQWRLITPIAETWAGRKEPELTEYEAGTDGPEAAQQLLARNGHH
                     WIRLSESTSACD"
     gene            complement(3287..4303)
                     /gene="gnd"
                     /locus_tag="VFO72_10165"
     CDS             complement(3287..4303)
                     /gene="gnd"
                     /locus_tag="VFO72_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET9479701.1"
                     /translation="MQIGMIGLGRMGANMVRRLLRANQECVVYDRNVETVRNLAAEGA
                     VGAESLKDFVAKLGPQRAIWLMIPAALVEATINDLVAIVSKDDIIIDGGNSYYVDDIR
                     RASELKGKGIHYVDVGTSGGVWGLERGYCQMIGGENDVVKHLDPIFRALAPGAGSVSP
                     TPGRDPGSSTATEGYVHCGPAGAGHFVKMVHNGIEYGLMAAYAEGLNVLHHANAGKTE
                     RTADAETTPLRNPEHYRYDFNIADITEVWRRGSVIASWLLDLTAIAFAEHPQLEGYSG
                     RVADSGEGRWTINAAVEEGVPVPVLSSALFQRFDSRGEAEFANKVLSAMRFQFGGHVE
                     RPAK"
     gene            4367..5152
                     /locus_tag="VFO72_10170"
     CDS             4367..5152
                     /locus_tag="VFO72_10170"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9479702.1"
                     /translation="MRQLSLLLLLVLASPAMAQRTPVLPAGRVVDLSHPFDAKTVYWP
                     TAESFKLDTDFEGVTDKGYFYSAYRYSAAEHGGTHLDAPVHFAKGRHTVDEIPLEQLM
                     GSGIVIDVVSECASNPDYQVSVADLRKWERRNGRIRPGTIVFLRTGFGKHYSNRKLYL
                     GTDERGAGAVAKLHFPGLDPAAARWLTQNRSIKAIGLDTASIDYGQSTLFESHRILFE
                     KNIPAFENVANLDQLPDKGFSVIALPMKIKRGSGGPLRIVAFR"
     gene            5158..5919
                     /gene="pgl"
                     /locus_tag="VFO72_10175"
     CDS             5158..5919
                     /gene="pgl"
                     /locus_tag="VFO72_10175"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9479703.1"
                     /translation="MELKVFTDSGEVARAAAQHWVSVKPRTVALSGGSTPRALYELLA
                     DPAEPFRAQVQWDSTHFFFTDERHVPPDHPDNNYRMVNESMFSRVPVPPANIHRILSE
                     NPVAHDAAEAYEHDLRRSFREAIPAFDLILLGLGEDGHTASLFPHSSALKETERLVVA
                     PWVKKLNAYRITMTLPVLNNGKSVMFLVTGDSKAEMLKEVFRTDKIPDLYPAQAISPT
                     SGEVSWFVDKAAARLCSTSNSSNAAAASDLLNKNP"
     gene            complement(5958..6611)
                     /locus_tag="VFO72_10180"
                     /pseudo
     CDS             complement(5958..6611)
                     /locus_tag="VFO72_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT         1428 a         2000 c         1805 g         1405 t
ORIGIN      
        1 tcgaaagaaa gacggcacga tcatcaacgt cgagatcacc gccaaccgcg tccacttcgc
       61 cggacgatcc gcagagtttg tcctggccca cgacatcacc gaacgtaagc aagcagaaat
      121 agcgctgcgc atcagcgaag atcgttatcg cgatctcgta gacaacagtc acgaattgat
      181 ctgcacgcac gatctcgaag gtaaggtgct ttccgtcaat ccgtgggcgg cgcgtatgct
      241 cggctacccg caggaagcgc tgatcgggat gaacattcgc gacggtctgc tgcccgaata
      301 tcgcgagcag ttcgaggaat acctgcacac ggtcaaaacg gagggctcgg cgcgcggcgt
      361 aatgaaagtg cgcaccgcga caggtgaaac gaggctttgg gagtactaca acacgctgcg
      421 aacggaagga gtcgaagtgc caatcgttcg cggcatggct cacgatgcca ccgaacggcg
      481 ccaggcgttg aagcgcgaac aggaagcgcg actcgaagct gaagccgcga atcgcgtcaa
      541 ggacgagttt ctttcaacgc tctcgcacga actgcgcaca ccgttgacgg caatcatggg
      601 ttggtcggat cttttgctgc acgatgaagt cgcacctcgt aagcgacgcc aggcgatcga
      661 aaccatcgcg cgcaatgcca actcccaatg ccaactcatc aacgacctgc tcgaagtctc
      721 gcgcatcatc accggaaaat tgcggctcga gtttgccgcg tgcgaactgg agtcggtaat
      781 ccaggccgcc gtcgacagta ttcgtccaac cgcggaagcg aaaggcgtca gactccaaat
      841 actgctcgat ccaaacgccg gtccaatctt tggcgatcgc gaacgccttc aacaagtcgt
      901 ttggaacctg ctgtccaacg gcgtcaagtt cacacgcgcc ggcggactgg tccaggtcaa
      961 actgcaacgc atcaactcgc acgtcgaggt tgtcgtcacc gacaccggtg ttggtatcaa
     1021 ctccgatttt ctgccgcacg tgttcgagcg gtttcgtcag gccgatggtt cgactacgag
     1081 aaactacggc ggcctgggac tcggcctcgc catcgtccgc catctggttg agctgcacgg
     1141 cggcaccgca tgggccgaaa gtggcggcca cgatgaaggg gccacattca ccgttcgctt
     1201 ccctgcgatg atcgaactcg agcagcgagt tgaaccaccg attgagacgc aaactgagtt
     1261 cgcggttgcg tcgcgcgatc gagaacgcac gttgcgaggg ctgcgagtgc ttgtcgtcga
     1321 tgatgaattt gacgcgcgcg tgttggtgac tacgatgttg gaacgttccg gcgcgcaggt
     1381 gctcgccgtt tcgtcaacac gggaagcact cgaatcgatg cagacgtgga gacccgacgt
     1441 gctgatcgct gacatcggca tgccggttga ggacggctac agtctgatca gaaaagtacg
     1501 cgcgctaccg agcgatcgag gcgggcaaat accggcgctg gccttgacgg cttacgcaag
     1561 aactgaagat cgcattcgag cactctccga aggttatcag atgcacttag cgaaacccgt
     1621 tgaccgcgtt gaactcgcca cgatcattgg cagtctgtgc cacagataac gcggccctaa
     1681 tcgcaggcgc tagtgctctc ggataaacga atccagtgat gaccgttacg cgccaacaac
     1741 tgttgcgccg cttccggtcc gtcagttccc gcctcatatt cggttaactc aggctccttc
     1801 cggccggccc acgtctctgc aatcggagtt atcaaacgcc actgcgcctc aacctcatcg
     1861 ccgcgcgtaa acagcatcgc atcgccgcgc atcacgtcga gcaacaacgt ttcgtacgct
     1921 gccggtggtt caacgccgaa cgccctctga taacaaaaat ccatggagac ggtcgtcgtc
     1981 cgcatttcga atcccggccg tttcgcgcca aacgtgacgg tgatgccctc gttcggttga
     2041 atgcgcagcg cgatgatgtt gggaagaatc tcgtcgtcgg gtgtgcgcgc gaacagcgcc
     2101 tgcggcgtac gtttcaagtg aacggcgatt tcagtgaggg tccgccccag gcctttgccg
     2161 gtacgcacgt aaaaaggaac tcccgaccag cgccagtttg agactctgaa ttcgatcgcg
     2221 gcgtaagttt ccgtgtgcga ctcgttctta acgccttcct cttcgcgata accctttact
     2281 cgttcgcctt taatcaaacc tggtccatac tgcgctcgca ccgtgcgcgc agcaacttca
     2341 tcgggcttta accggcgaat cgatcgcagc acctgcactt tctcttctct gacggaatcg
     2401 gcatcgaagg cgacgggtgg ttccatcgct gtcagcgtga gtagttgcag caagtgattc
     2461 gcaaccatgt cgcgcagagc gccggcctct tcgtaatagc cggcacgact cccgacaccc
     2521 gcagtttcag cagcggtgat ctcgacgtag tcgacgtagt ttcgattcca gatcggttca
     2581 aacatcgtat tgccaaagcg aaagacgagg atgttctgca ccgtgtcttt gccgagatag
     2641 tgatcgatcc gatagatctg gttctcggag aacacctcag cgatcttcgc gttcaacgct
     2701 tgagccgatt cgagatcgct gccgaaaggc ttttcgacga cgatgcggct ccagtgatcg
     2761 tggtcctgtt tggccaaacc tgctttaccg agtccttcga caatcaccgg cgccagcgat
     2821 ggcggcgtag caagatagaa aaggtgattg ttgctcgcgc ctttgtggcc cagctcttca
     2881 agtttctcag cggttttcgc ataggtgttg tcgtcgtcga gctctccggt ggtgtagaaa
     2941 atgcgttgcg caaacttccg ccactccgcg tcttcctctg gtgcgagctt ttctgactcg
     3001 agcagcgcct tgcgcacgaa cgcgcgatat tcgtcgtcag tcatcgggct ccggcccaca
     3061 ccgagaatct gcacactctc gagacagcct tcgtttctca ggttccacaa ggccggcatc
     3121 aacttgcgtc gcgtgagatc gccggtggcg ccgaagatca ccgtgacgca cggttctgcc
     3181 ttcggcagtg ccggcgcttc catgtgaatg ggctctggtt ctaccagtat cgttgacata
     3241 gttctctccc cctgggagga caagcctcaa actgcatttg acatccctac ttagctgggc
     3301 gttcgacgtg tccgccgaac tgaaatctca tcgccgacag aactttgttt gcaaactcgg
     3361 cctcgccccg cgaatcgaat cgctgaaaca atgcactact caaaaccggc accggcacgc
     3421 cctcctcgac cgccgcgttg atggtccaac gtccttcgcc cgaatcggca acgcgcccgg
     3481 agtagccctc gagctgcgga tgctcggcaa acgcgatcgc cgtcaaatcg agcagccatg
     3541 aggcaatcac gctgcctcgt cgccacacct ccgtgatatc cgcgatgttg aaatcgtaac
     3601 ggtaatgctc gggattgcga agcggcgttg tttccgcgtc cgcggttcgc tccgtcttgc
     3661 cggcgttggc gtgatgtaag acgttaagcc cttcggcata agcggccatc aacccgtact
     3721 caataccgtt gtggaccatc tttacgaaat ggccggcgcc cgccggacca caatgcacgt
     3781 agccttcggt tgccgtactc gaacccgggt cgcgtccagg cgtgggcgaa acgctgccgg
     3841 cacccggagc gagagctctg aagatcgggt cgagatgctt cacgacgtca ttctcgccgc
     3901 cgatcatctg gcagtagcca cgttcgagtc cccacacgcc gccgctggtg ccgacgtcaa
     3961 cgtagtgaat gcctttcccc tttagttcac tcgcgcggcg aatgtcgtcg acgtagtagg
     4021 agttaccgcc gtcgatgatg atgtcgtctt tactgacaat cgcgacgaga tcattgatcg
     4081 tggcttcaac aagagccgcc gggatcatca accagatcgc ccgctgcggt cccaacttcg
     4141 ccacgaaatc tttcaatgac tccgcgccca ctgcgccctc cgcggcgaga tttcgcaccg
     4201 tttcgacatt gcggtcgtaa acaacacact cctgattcgc gcgcaaaaga cgtcgcacca
     4261 tgttggcgcc catacgtccc agtccgatca ttccaatttg cattcgcagc tccgatccgc
     4321 gttaatcttt tggacgattt tactcaaagc ttaaggagcc ctcctaatga gacagttaag
     4381 cttactctta ttgttagttc ttgcctctcc ggcgatggcg caaaggacgc ccgttttacc
     4441 cgcgggccgc gtggtcgatc tgtcgcatcc gttcgacgcc aagaccgtct attggcccac
     4501 tgccgaatca ttcaaactcg acacggactt cgaaggcgtc accgataaag gttacttcta
     4561 ttcagcttat cgatactcgg cggcggaaca cggcggcacg catctcgatg cgccagtcca
     4621 ttttgcgaag gggcgacaca ctgtcgacga gatcccattg gaacaactga tgggcagcgg
     4681 gatagtaatc gacgtcgtta gcgagtgcgc gtcgaaccct gattatcaag tcagcgttgc
     4741 ggatcttcga aagtgggaaa gaaggaatgg acgcatcagg cccggtacga tcgtttttct
     4801 gcgcacgggc ttcggcaaac actattcaaa ccgcaagctg tatctcggca ctgacgaacg
     4861 tggcgcgggt gcagtcgcaa agcttcattt tcccgggctg gatccggcag ccgcgcgctg
     4921 gttgacacag aaccggtcca tcaaggcgat cggcttggac actgccagca tcgactacgg
     4981 gcagtcgaca ctgtttgaga gtcatcgcat cttgtttgag aagaacattc cggcattcga
     5041 gaacgttgca aatctggacc agttgcctga caaaggtttt tcggtgattg cgttgccgat
     5101 gaagattaag cgtggcagtg gcgggccttt gcgaatcgtt gcgtttcggt aaagcggatg
     5161 gaactgaaag tctttacgga ttcaggagaa gttgcacgcg cggcggcgca acattgggtg
     5221 agcgtaaagc caagaaccgt tgcgctgtca ggtggctcga cgccgagggc gttgtacgaa
     5281 ctgctggctg atccggcgga gccgtttcgt gcgcaagtcc agtgggacag cacacatttc
     5341 ttcttcaccg acgaacgcca cgtgccaccg gatcatccgg acaacaacta tcgcatggtg
     5401 aatgaatcga tgttttcgcg cgtgccggtg cctccggcaa acattcaccg catcctctcc
     5461 gaaaaccctg ttgcccacga tgccgccgaa gcatacgagc acgatctgcg tagatccttt
     5521 cgcgaagcga tcccggcctt tgatctgatt ctcctgggcc tcggcgaaga cggccacacg
     5581 gcttcactct tcccccactc ttccgcgtta aaagaaacag agcgtctcgt tgtagcgccg
     5641 tgggtgaaaa agctgaacgc ctatcgaatt acgatgacgc tgccggtgct gaacaacgga
     5701 aagtcggtga tgtttcttgt cactggagat tccaaggccg agatgctgaa ggaagtcttc
     5761 cgtacggata aaatcccgga tctttatccc gctcaggcaa tctccccaac cagtggcgaa
     5821 gtgtcctggt tcgttgataa agcagccgcg cgcctttgct ccaccagcaa ctcttcgaac
     5881 gccgcggccg ccagcgactt actgaacaag aatccctgaa ccacgtcaca accaagcgaa
     5941 ctcagatact gaagctgctc ctgagtctcc acgccttccg caatcacagt cagatcgagc
     6001 gcgtggccca acgtgatgat cgctcgcacg atcgccgtgt cgtgcggatc cttgcaaacg
     6061 tcagccacaa acgtcttgtc gatcttcaac gtgtcgattg gaaagcgctt gaggtaattc
     6121 agcgacgaat agccggtgcc gaagtcgtcg atcgccactc tgatccccat caacttcagc
     6181 tcgtgcaact tctcgatcgc ctgctcgggc tccttcatga tcgaactctc tgtcaactcc
     6241 agctccagcc aacgcggatc gaggttcgtc tctttcagaa tctcggcgac cgtcgagatg
     6301 aacgccggct gctgaaactg gcgagccgag aagttcactg acagacgcat cggcgccagt
     6361 cccgcatcct gccatgactt gttctgcaga caagcattcc gcatcaccca atcacccagc
     6421 gcgatgatca atccgctgtc ctcggccagc ggcacaaact cactcggata aagcattccc
     6481 aaactcgggt gccgccagcg aaccaacgcc tccatgccga cgagatgaaa atcaggaatt
     6541 gcgacctgcg gttgatagtg aacaacaaac tcttctcgct cgagtgcgcc gcgcaggttg
     6601 ctttcgagca cgagttgttt gagcgcgcgc gtagtgcc
//
LOCUS       DASTEY010000579         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_425192, whole genome shotgun sequence.
ACCESSION   DASTEY010000579 DASTEY010000000
VERSION     DASTEY010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425192"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /gene="purH"
                     /locus_tag="VFO72_10185"
     CDS             <1..1489
                     /gene="purH"
                     /locus_tag="VFO72_10185"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET9479704.1"
                     /translation="GIIDFARELKNFNVEIISTGGTAKALRDAGIDVRDISDVTGFPE
                     MMDGRVKTLHPRVHGGLLALRDNPDHVAAMQQHDIQPIDMVVVNLYPFAETISRKDVT
                     REEAIEQIDIGGPAMIRSAAKNAAGVAVVVDPSQYPQLIKELHQNAGSISIATLQRFA
                     TQAFQQTAQYDAMVSSYLAGGVGQFPSQITSTLTKLSDLRYGENPHQLAALYRTASGN
                     GLANAAILSGKEMSFNNYVDADAAWQLVCDFHDTACAIIKHTNPAGVALGETPAEAYQ
                     KALATDPVSAFGGIVAFNRSVDLPAAQEVTKIFTEVVIAPDYDAAALELLQTKKNLRV
                     IRMSSADRNDELEFRQISGGMLVQTKDVHELRQEDLRVVTERQPTDEEIRALLFAWTV
                     CKHTKSNAIVYARADQTAGVGAGQMSRVDSVKIGAMRARLPVAGSVLASDAFFPFRDG
                     LDEAARHGITAVIQPGGSVRDDEVIAAANEHGLAMVFTGVRHFKH"
     gene            1585..2541
                     /locus_tag="VFO72_10190"
     CDS             1585..2541
                     /locus_tag="VFO72_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9479705.1"
                     /translation="MSDNENSIRCRYCGQRNQIRLDAGDARCGRCRLPLSEAPHKKFA
                     RLDKDQYIHPDDSRALAALRAIPGIDSALKKLLAVTGESAIRVIFTASAVKVTPTQCP
                     DLYAKLQIACTTLGVDMPELFVQQNPIVNAFTGGVERPVIVLYSQLIERLSDDEVLAV
                     VAHEVGHIHAEHVLYLTAARLIEFLAKSAVLASPLTFIVKELLTMSMRAALLAWARRA
                     ELSCDRAALLVTQDANIIGRTMMKLSGGTYASRVDYDQFLAQARDFQKNYDEKALDRF
                     WADVITSGLTHPFPVWRVSEILQWVESGEYKALMTSPESAAA"
BASE COUNT          601 a          768 c          700 g          539 t
ORIGIN      
        1 tggtatcatc gacttcgctc gcgaactcaa aaacttcaac gtggaaatca tcagtaccgg
       61 cggtactgct aaagcactgc gcgatgccgg catcgacgtg cgcgatattt ccgatgtcac
      121 tggttttccg gagatgatgg acggccgcgt caagactttg catcctcgcg ttcacggagg
      181 actgctggcc ttgcgcgata acccggatca cgtcgccgcc atgcagcaac acgacatcca
      241 gccgatcgac atggtcgtcg tgaatctcta tccgttcgcg gaaacgatca gccgcaaaga
      301 cgtcacgcgc gaagaggcaa tcgaacagat cgacatcggc ggtcctgcaa tgatccgctc
      361 cgctgcgaag aacgcggccg gcgtcgccgt cgtcgtcgat ccatcacaat atccgcagtt
      421 aattaaggag ctacatcaaa acgccggctc catctcaatc gcgacccttc aacgtttcgc
      481 tactcaggcc tttcagcaaa cagcacaata tgacgcaatg gtaagttcgt acctcgccgg
      541 cggagtcgga cagttccctt cacaaataac ttcgacgcta acgaagcttt cggatctccg
      601 ttacggggaa aacccgcacc agcttgcagc gctctaccgc accgcttcgg gcaacggtct
      661 cgccaacgct gcgatcctgt ccggcaagga aatgtcattc aacaactacg ttgacgccga
      721 cgcggcgtgg caactcgtct gcgattttca cgacaccgcc tgcgcgatca tcaaacacac
      781 aaatccggcc ggtgtcgcac tcggcgaaac tcctgccgag gcgtatcaaa aagcactggc
      841 aactgatccc gtgtcagctt ttggcggtat cgtcgctttc aaccggtcgg tggacttacc
      901 ggccgcgcag gaagtaacaa agatcttcac cgaagtcgtc atcgctccgg attacgatgc
      961 ggccgcactg gaactgctgc aaacgaaaaa gaacctgcgt gttattcgca tgagttctgc
     1021 cgatagaaac gacgaactcg agttcagaca gatctcgggc ggcatgctcg tccaaacaaa
     1081 agacgtccac gagctgcgcc aggaggatct aagagttgtc accgagaggc agcctacaga
     1141 cgaggagatt cgggccttgc tcttcgcgtg gaccgtctgc aaacacacaa agtcaaacgc
     1201 gatcgtctac gcgcgtgctg accagacagc cggcgtcgga gcgggacaga tgtcacgcgt
     1261 tgattcggtg aagatcggtg cgatgcgcgc gcgtttgccg gtcgccggtt cggtgctcgc
     1321 atcggacgct ttcttcccct ttcgcgatgg tctcgacgaa gccgctcgcc acggcattac
     1381 ggctgtaatt cagccgggtg gctcagtacg agacgacgaa gttattgccg ccgcaaacga
     1441 acacgggctg gcaatggtct ttacaggtgt gaggcatttc aaacactgat attatgattg
     1501 ccgattgcca attgccaatt tccgatttgt aagagcaata cgcgaaaatc cggccattcg
     1561 gcaatcggca atcaaaaatg acctatgtct gataacgaaa actcgatccg ctgccgttac
     1621 tgcgggcagc gcaaccaaat acgtttggac gcaggcgacg cgcgctgtgg ccgctgccgg
     1681 ctgcccttat ctgaagctcc acacaagaag tttgcgcggc tcgataagga ccaatacatc
     1741 catccggacg atagccgcgc gctcgctgca ctgcgcgcaa tacccggaat tgattcggcg
     1801 ctgaaaaaac ttctcgccgt cacgggcgaa tcagccatcc gcgtgatctt cactgccagc
     1861 gccgtgaagg tgacaccgac acagtgtccg gatctttacg ctaagttgca gatcgcctgc
     1921 acgacgctgg gcgtcgacat gccggaactc ttcgtgcaac agaacccgat cgtgaatgcg
     1981 tttacgggcg gcgtcgaacg tccagtgatc gttttgtact cgcaactgat cgagcgcttg
     2041 agtgatgacg aagtgcttgc cgtcgtggcg cacgaagtcg gacacattca cgccgagcac
     2101 gttctttact tgaccgccgc tcgtttgatc gagtttttgg caaagagcgc tgtgctcgcc
     2161 tctccgctga ccttcatcgt gaaagaacta ctgacgatga gcatgcgcgc agcactactc
     2221 gcctgggcgc gacgtgccga actgtcctgc gatcgcgccg ccttgctggt cacgcaggac
     2281 gcaaacatca tcggccgcac gatgatgaag ctgagcggtg gcacgtatgc ttcgcgcgtt
     2341 gattacgacc agtttctcgc tcaggcgcgt gacttccaga agaactacga cgagaaggca
     2401 ctcgatcgtt tctgggccga cgtgatcact tcaggcctga ctcatccatt cccagtctgg
     2461 cgcgtctcgg agattttgca gtgggttgaa agtggtgaat acaaagctct catgacttcg
     2521 cctgaaagcg cagccgctta agctgcacgc aacaacttaa tgctcgaaaa caaaaacgga
     2581 atcattttcg gtgtggccaa caagcgct
//
LOCUS       DASTEY010000580         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1133650, whole genome shotgun sequence.
ACCESSION   DASTEY010000580 DASTEY010000000
VERSION     DASTEY010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133650"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFO72_10195"
     CDS             complement(<1..1187)
                     /locus_tag="VFO72_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET9479706.1"
                     /translation="MASRQLIRIGVDTGGTFTDFVFERDGRFNLFKLPSTPSDPSLAI
                     RQGLIRICDETGTRLDRIEVVHGTTVGTNALLQRRGARIALITTKGFEDVLAIGRQAR
                     PELYNLNAIKPQPLVADELRLGISERVVASGEVLEPLDERELALLVKKLKRTGVEAVA
                     ISLLFSFLHPEHEERIASALTPLDIPLSISSRILPEYREYERTSTVVINAYLQPLMGR
                     YLKRLSETNTEKLNLRVMQSSGGSISADVAATEPVRTILSGPSGGVVGALRAAQSAGI
                     QNVITFDMGGTSTDVALCDSGAIRTTNEATVAGLPVAVSVMDIHTVGAGGGSIARVDE
                     GGSLRVGPESAGADPGPACYGRSLLPTVTDAHLVLGHFGGTGLLGGEFKLDEKRAREA
                     MQKL"
     gene            complement(1177..2271)
                     /locus_tag="VFO72_10200"
     CDS             complement(1177..2271)
                     /locus_tag="VFO72_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9479707.1"
                     /translation="MKKLPVGIGIIGTGFARTTQIPGFRDCMGAKIVAIASRNRERAA
                     AIAKEFGIEHVANDWQELVALNDVDLVSVVTPPSTHMEITLAALEQRKAVLCEKPMAL
                     NAREAARMVEKAKSAGVLALIDHELRFLNSRRVMRGMLESGAIGSVRHCNYVFRSDYR
                     SLADRAWDWWSDEAMGGGALGAIGSHVVDSFRWMLSTEVTKVLGMLTTHIKERRDKPN
                     GGMRAVTTDDEAKLLFRFADGQHTQAATGAASISVIESGKYENRFEIYGTRGALMVEE
                     TGELWRSPTGSGTWRPVQVDQDHMASGMREGSWSRGFTAFSVAICEAMRAGKTTVKDA
                     ATFEDGYRVQLVLDAIRASNESECWVTVGQ"
BASE COUNT          505 a          719 c          663 g          508 t
ORIGIN      
        1 agcttctgca tcgcttcgcg ggcgcgcttt tcgtcaagct tgaattctcc gcctaacaaa
       61 cctgtgcctc cgaagtgccc gagcacgagg tgggcgtcag tcacagtcgg caataacgag
      121 cgtccataac aagctggtcc aggatcggct cccgctgact ctggtccaac acgcaacgag
      181 ccaccttcgt ccactcgcgc gatcgatcct ccgcctgcgc caacggtatg gatgtccata
      241 acggaaacag ccaccggcaa gccggcaacc gtcgcctcgt tcgtcgtgcg aatcgcgccg
      301 ctatcacaca acgcaacgtc ggttgacgtt ccgcccatat caaacgtgat gacgttttga
      361 attccagccg attgggccgc gcgcaaagcg ccgacgacac cacccgaagg cccggacagg
      421 atcgtgcgaa cgggctccgt cgcagccacg tcagcagaga tgctgccgcc ggatgattgc
      481 atcacgcgca agttgagttt ttctgtgttc gtctccgaca gtcgtttcaa ataacggccc
      541 atcaacggtt gcaggtaagc attgatgacg accgtcgagg tgcgttcgta ttcgcgatat
      601 tcgggaagga tgcgactcga gatggacaac ggtatatcaa gtggagtaag cgctgaggca
      661 atacgctcct cgtgttccgg atgcaggaag gaaaacaaaa gcgagatcgc taccgcttca
      721 actccagtgc gcttcagttt ctttactagc agggcgagct cacgttcatc cagcggctca
      781 agcacttcgc ccgacgcaac cacgcgctcg cttatcccta acctgagctc gtcagcaacc
      841 aggggctgcg gcttgatggc gttgaggttg tagagctcag gccgagcctg gcgcccgatc
      901 gcgagtacgt cctcgaagcc tttagtggta atcagagcga tgcgggcgcc gcgacgttgc
      961 agcaaagcgt ttgtgccgac agtcgtgcca tgcacaactt cgatccgatc gagtcgcgtc
     1021 ccggtctcat cgcagatgcg aataagtccc tgccgaatag ccagtgaagg atcggaagga
     1081 gttgaaggta gcttgaagag gttgaaacgc ccgtctcgct cgaagacgaa atctgtaaac
     1141 gtgccgccgg tgtcgacacc gatgcgtatg agttgtctac tggccaaccg ttacccagca
     1201 ctcactttca ttcgaagcac gtattgcatc cagcacgagt tgtactcgat agccgtcttc
     1261 aaaagtcgca gcgtctttca cagttgtttt gcccgcccgc atcgcttcgc agatagcgac
     1321 tgaaaatgcg gtgaagcccc tggaccaact tccctctcgc atcccactgg ccatgtggtc
     1381 ctggtccacc tgcacgggac gccatgtgcc cgaaccggtc ggcgatcgcc acagctcgcc
     1441 tgtctcttcg accattagcg cgcccctggt gccgtagatc tcgaaccggt tttcgtactt
     1501 gccggattcg atgactgaaa tcgaagcggc gccggtggct gcctgcgtgt gttgaccatc
     1561 agcgaaccgg aacaggagtt tggcttcgtc gtcagtggtg accgcgcgca tgccgccgtt
     1621 gggtttgtca cgacgctctt tgatgtgagt cgtcaacatg ccgagcacct tcgtcacttc
     1681 ggtgctcaac atccagcgaa acgaatcgac gacgtgcgat ccgatcgcgc ccaatgcgcc
     1741 gccgcccatc gcctcatccg accaccagtc ccacgcgcga tcggcgaggc tgcggtagtc
     1801 agaccgaaac acgtaattgc aatgacgtac cgagccaatc gcgccacttt ccagcatgcc
     1861 gcgcattacg cgccggctgt tgagaaagcg cagctcgtga tcgatcaacg ctagtacgcc
     1921 ggctgacttt gctttctcga ccatgcgagc agcttcgcgg gcgttcaacg ccatcggttt
     1981 ttcgcagagt actgctttgc gttgctcgag tgccgcaaga gtgatctcca tgtgagtgga
     2041 tggaggcgtg acgacgctga cgagatcgac gtcgttgagt gcgacgagct cctgccaatc
     2101 gttggcgaca tgttcaatgc caaattcttt agcgatggct gcggcgcgct ctcgattgcg
     2161 actcgcaatc gcgacgatct tggcacccat gcaatcacga aaacccggga tttgggtagt
     2221 gcgggcaaac cccgtcccga taattccgat tccaaccggt aacttcttca tgacggcaat
     2281 actttatcat acggttaata ggtcctatag gacctatagg accgattaag ggattgaaaa
     2341 tttatcaatc gcgcccggcg aggcgggcga ccatgtgtac gagttggctg gcgcc
//
LOCUS       DASTEY010000581         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1133677, whole genome shotgun sequence.
ACCESSION   DASTEY010000581 DASTEY010000000
VERSION     DASTEY010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133677"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="rph"
                     /locus_tag="VFO72_10205"
     CDS             <1..729
                     /gene="rph"
                     /locus_tag="VFO72_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET9479708.1"
                     /translation="MSFARTDGRAPDELRRVCITPGFLPYAEGSVLIAMGNTRVVCSA
                     SLEDRVPPFLRNQGHGWLTAEYSMLPRATQTRTARETGRGGPSGRTHEIQRLIGRSLR
                     AVADMKVLGERTITIDCDVLQADGGTRTAAITGAYVALALASQQLVKAGKIQRSVVTD
                     QVAAISVGIVGNTPLLDLKYDEDSRAEVDMNVVCTGDGRFIELQGTAEREPFSRAQMD
                     ELVALGTRGIEALIAIQKKVIAQA"
     gene            817..1323
                     /locus_tag="VFO72_10210"
     CDS             817..1323
                     /locus_tag="VFO72_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479709.1"
                     /translation="MRRILAFTLVLIGLMAPVALAQQLYTHEKVDYSFELPSPTWKAI
                     LEPDAAHEHPEFVYGDRLDGFLTIRKEVVDAGTSPAALARRDQDLRLRFLPGFVEGKE
                     ENFDGRLDGVTISYEFVRTGKPMLGRTYYLQADPRTIYALRFTGLRDKISRIRNQTDL
                     IARTFKLK"
     gene            1326..1736
                     /locus_tag="VFO72_10215"
     CDS             1326..1736
                     /locus_tag="VFO72_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HET9479710.1"
                     /translation="MKLAHGLAIKPSTIHGRGCFASVPFKKRHKIAEYTGEKISNAEA
                     RRRSHRRMLRICEVNNRWSLDGSRGGNGTHYINHSCTPNAFMQILYDHILFIALRDIE
                     AGEEITIDYQSTLHSDDKRCICGARTCRGTINIV"
     gene            complement(1745..>3068)
                     /locus_tag="VFO72_10220"
     CDS             complement(1745..>3068)
                     /locus_tag="VFO72_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479711.1"
                     /translation="ATGENSSTVAVPLLHEGETAGVLLIRLASEFSVDDTPLLEAVSS
                     QFARNLERESFSKSAAHTQSLSYFSQSGSRRKLDALNVLKAVTVEQRCEASTLSHIDD
                     GVAIAYLDGTLALVNPSLLSFAGLTSEQAAKMDLFDLLHKFRTDIFDEPEIAVRRVLQ
                     TGVEYEGELNFEPKNQILNLRISLLRERLNQKDETPIGIAIHVKDLTSTKEYEKLKSD
                     MISLMSHELRTPLTSINGFAELLTADQTIPAQAREFVSIIANESQRMSRMINTFLSVT
                     QLQRKDKQEVLKIPLRLDEVVRETIASLQPVAKKKRIRLLEQPAHRIPPVAADKSLIT
                     QAVKNLVNNAIKYSPERTTVTVSTALEAEAVRVCVEDRGFGIPAEAKERVWDKFYRVV
                     REGQEKDEESTGLGLSFVREVVEQHGGHVELDSVEGRGSKFSFTLPRL"
BASE COUNT          681 a          866 c          839 g          682 t
ORIGIN      
        1 atgagcttcg cacgcactga cggacgcgct cccgacgaat tgcgccgagt atgcatcacg
       61 ccgggtttcc tgccatacgc ggaaggttcg gtcctgatcg cgatgggaaa cacgcgcgtg
      121 gtctgttccg cgtcgctcga agatcgcgtg ccgccgttcc tgcgaaacca gggacatggt
      181 tggttgaccg ccgaatactc catgttgccg cgtgcaaccc aaacgcgaac ggcgcgagag
      241 accggccgcg gcggaccttc cggccgcact cacgaaatac agcgactgat cggcagaagt
      301 ctccgtgcag tagcagacat gaaggtgctt ggcgaacgca cgatcacgat cgactgcgac
      361 gttctgcaag ctgatggcgg aactcgtacc gcggcgatca ccggcgctta cgtcgcttta
      421 gctttggcga gccagcagct ggtgaaggcc ggcaagattc aacgatcggt ggtgacggat
      481 caggttgcgg cgatcagtgt gggtattgtc ggcaacacac cgttattgga tctcaagtac
      541 gacgaagatt ctcgcgccga agtcgatatg aatgtggtgt gtaccggcga cgggcgtttc
      601 atcgagctgc aaggaacagc ggagcgtgag cctttcagcc gcgcacaaat ggatgagctg
      661 gtcgcgctcg gcacgcgcgg aattgaagcg ctcatagcga ttcagaaaaa agtgatcgcg
      721 caggcttaat aggtccctta ggtcttatag gacttatctg agcaacgacc acactggacg
      781 ccggcatctg aacctgcaag tttttggagg catttaatgc gacggattct cgcttttacc
      841 ctggttttga tcggattgat ggcgccggtc gcgctggcgc aacaactcta cacacacgag
      901 aaagttgact acagtttcga gcttccgtcg ccaacgtgga aagcgattct cgaacccgat
      961 gccgctcacg aacatcccga atttgtttac ggcgatcggc tcgatgggtt ccttaccatt
     1021 cgtaaagaag tagttgatgc agggaccagc cctgcggcac ttgcgcgccg cgatcaggat
     1081 ctgagactgc ggttcctgcc cggcttcgtc gagggtaagg aagagaattt tgacggcagg
     1141 ctcgatggcg taacgatctc ttacgaattt gtcaggaccg gcaagcccat gctcggtcgc
     1201 acttattact tgcaggccga cccacgcacc atctacgcac tgcgcttcac cggcttgcgc
     1261 gacaagataa gtcgcatccg taaccaaacc gatctgatcg cgcgcacctt caaactcaag
     1321 taaaaatgaa gctggcccac ggactagcaa tcaagccttc aaccatacac ggcaggggct
     1381 gcttcgccag cgtccctttc aaaaaaagac acaagatagc tgaatacacc ggtgagaaga
     1441 tctcaaacgc cgaagcccgg cgcaggtctc accggcgaat gctccgcatc tgtgaggtca
     1501 acaatcgctg gagtcttgac ggcagccgcg gcggcaatgg cactcactac atcaatcact
     1561 cctgcacgcc aaacgccttc atgcaaatcc tctacgatca cattctcttc atcgccctgc
     1621 gcgacatcga agcgggcgaa gagataacga tcgattacca atcaacactg cattcggacg
     1681 ataaacgttg catctgtggc gcgaggactt gtcgcggcac gatcaatatt gtgtgacttt
     1741 ctgatcagag tcgcggcagc gtgaaactaa acttcgatcc acgtccctct actgaatcga
     1801 gttcgacgtg gccaccgtgt tgctcgacca cttcgcgcac aaacgataat cccaggcccg
     1861 tcgactcttc gtctttttcc tgtccttcac gcacgacgcg atagaacttg tcccaaacac
     1921 gctctttcgc ttcagcagga atcccgaaac cacgatcctc aacacaaacc cgcactgctt
     1981 cagcttcgag cgccgtcgaa accgtgacgg tagtacgctc aggactgtac ttgattgcgt
     2041 tgttcaccag gttcttcact gcctgcgtga tcagactctt gtccgcggcc actggcggaa
     2101 tgcgatgtgc aggttgttcg agcaagcgaa tgcgtttctt cttagccact ggttgtagcg
     2161 aagcgatcgt ttcgcgcaca acttcgtcga gacgcagcgg aattttcaac acttcctgct
     2221 tgtctttgcg ctgcaactgc gtcacggaca ggaaggtatt gatcatacgc gacatgcgtt
     2281 gcgactcgtt cgcgatgatc gatacaaact cccgtgcctg cgcgggaatc gtctggtcag
     2341 cggtcagcaa ctctgcaaat ccgttgatgc tcgtgagcgg cgtgcgtagc tcgtgcgaca
     2401 tcagtgagat catgtcgctc ttcagcttct cgtactcttt agtcgacgtc agatctttaa
     2461 cgtgaatagc aatgccgatc ggtgtttcgt ctttttggtt cagacgctcg cgcagcagcg
     2521 agatccgcag gttgagaatc tggttctttg gctcgaagtt gagctcgcct tcgtactcca
     2581 cgccggtttg taatactcgt cgcaccgcga tctcaggctc gtcgaaaatg tcggtgcgga
     2641 atttgtggag caagtcgaag aggtccatct ttgcagcctg ctcggaagtt agtccagcga
     2701 aactcagcag agatggatta acgagcgcga gcgtgccgtc gagataagca atcgcgacgc
     2761 catcgtcgat gtgagagagt gtgctggctt cacatcgctg ctcgactgtt acagctttca
     2821 acacgttcag cgcgtcgagc tttcgtctgc tgccgctttg tgaaaagtaa ctgagcgatt
     2881 gtgtgtgcgc cgcactcttc gaaaagcttt cgcgctcgag attgcgagcg aactgggagc
     2941 tcactgcttc cagcaacggc gtgtcgtcga cggagaattc ggaagccaga cgaataagca
     3001 acacaccggc agtctcgcct tcatgcagca gtggaactgc gactgtggat gagttctcgc
     3061 cggtcgct
//
LOCUS       DASTEY010000582         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1545612, whole genome shotgun sequence.
ACCESSION   DASTEY010000582 DASTEY010000000
VERSION     DASTEY010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545612"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFO72_10225"
     CDS             <1..183
                     /locus_tag="VFO72_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479712.1"
                     /translation="RWRVVVPPVMLVYCLLVRGGIFDGWAGFYYAFQRTTAELMLAVN
                     LGRRFTRINADVYSRY"
     gene            164..1894
                     /locus_tag="VFO72_10230"
     CDS             164..1894
                     /locus_tag="VFO72_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9479713.1"
                     /translation="MYILGINAYHGDAAAAIVRDGKLIAAVEEERFNRFKHSAGFPTQ
                     AIKYCLEAAGIGLEDLDHVGISRDPSAHLHKKILFAATRLTKLASQAKDRFGNAAKVR
                     DVKDELAHVFGVSKKQLRARFHNIEHHRAHLASCFLVSPFNKSALLSIDGFGDFISTM
                     WATGEGNSIDVLGQVEYPHSTGIVYTATTQFLGFSHYGDEGKVMGLAPYGEPRFIREF
                     REIIRTEENGQFRLNLDYFRHHAEGVEMTWDEGSPVIGRVFSDEFAHRFGPPRAPGAA
                     LSERDRDIAASLQLRLEEVGFHILNHLHERTGLTDLGLAGGVAYNSVMNGKILLNTPF
                     KRLFIQPAAGDSGTALGVCYQIYNGILNCERGEVMAGAYTGPQFTREEIKKALSESEL
                     AFECYSDGQLTKRAAQDIAAGLVVGWFQGRMEFGPRALGNRSILVDPRRAEMKDILND
                     RIKKREPFRPFAPSILEERVGDYFEQTHPAPTMLMVYQFKPQRRGEIPAVTHVDGSGR
                     LQTVSRAVNERYYQLISDFAALTGVPVVLNTSFNENEPIVCTPQHAIECFRKTKMDVL
                     YLGNFAVRRE"
     gene            1906..>2718
                     /locus_tag="VFO72_10235"
     CDS             1906..>2718
                     /locus_tag="VFO72_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET9479714.1"
                     /translation="MRILVTGGAGFIGSHLCERLVSEGNEVLCLDNFFTGRRENIFHL
                     LDNPRFELLRHDVTEPILLEVDQIYNLACPASPVHYQYNPVKTVKTSVMGAINMLGLA
                     KRVRARILQASTSEVYGDPLVHPQTEDYWGNVNPIGLRSCYDEGKRLAETLMTDYHRQ
                     NKVDIRIARIFNTYGPRMLENDGRVVSNFIVQALRGQPLTLYGEGQQTRSFCYVDDLL
                     EGLIRLMNTENQHDPVNLGNPGEFTIRQLAEEVIKTCESKSSFTYMPLPADDP"
BASE COUNT          585 a          728 c          805 g          600 t
ORIGIN      
        1 cgttggcggg ttgtggtccc gccggtgatg ctggtttatt gcctattagt tcgtggcggg
       61 atctttgatg gctgggcggg gttttactac gcgttccaaa gaactacagc ggagttgatg
      121 ctggcggtta atttgggccg cagatttacg cggataaacg cagatgtata ttctcggtat
      181 taacgcgtat cacggcgatg cggcggcggc gattgtgcgt gatgggaagt tgatcgccgc
      241 ggtggaggag gagcgcttca accggttcaa gcactccgcg ggcttcccaa cgcaggcaat
      301 caaatactgt ctcgaggctg caggaattgg gcttgaagat ctggaccacg ttggtatctc
      361 cagagatccg tcggcgcacc tgcacaagaa gatcctgttt gcagccacac gacttacgaa
      421 actggccagt caagccaaag atcgattcgg gaacgctgcg aaagtacgtg acgtgaagga
      481 tgagcttgca cacgtgtttg gggtttcaaa gaaacaattg cgcgcacgct ttcataacat
      541 cgagcatcat cgcgcacatc ttgccagttg ctttttggtc tcaccgttta acaaatccgc
      601 gctattgtcg atcgacggct ttggcgattt catctcgacg atgtgggcca ccggcgaggg
      661 aaactcgatc gacgtcctgg gccaggtcga gtatccacac tcgaccggta tcgtttacac
      721 cgcaaccacg cagtttctcg gcttctccca ttacggcgac gagggtaaag tcatgggcct
      781 cgcgccgtac ggtgagccgc gattcatcag agagtttcgc gagatcattc gcacggaaga
      841 gaacggacag tttcgtttga atctcgacta ctttcgtcat cacgctgaag gtgtcgagat
      901 gacgtgggac gagggctcgc ccgtaatcgg ccgcgtcttc tcggatgagt ttgcccatcg
      961 cttcgggcca ccgcgagcgc ccggcgccgc cctctcagaa cgcgaccgag acattgcggc
     1021 cagcctgcaa ttgcggctcg aagaagtcgg cttccacatt ctgaaccatc ttcacgagcg
     1081 caccggactg actgacttag gtctcgcggg cggcgtggct tacaactcgg taatgaacgg
     1141 aaagattttg ctgaacactc cgttcaagcg gcttttcatt caaccggcgg ctggcgacag
     1201 tggaacggcg ctcggcgttt gttaccagat ctacaatggc atcctgaact gcgaacgcgg
     1261 cgaggtgatg gctggcgctt acaccggtcc acaattcacc cgcgaagaga tcaaaaaggc
     1321 attgtctgaa agtgagttgg ctttcgagtg ttactcggat gggcagctga caaagcgcgc
     1381 ggcacaggac atcgcggcgg gtttagtcgt aggatggttc cagggacgca tggagtttgg
     1441 cccacgcgcg ctgggcaatc gttcgatcct cgtcgatcca cggcgcgccg agatgaaaga
     1501 tatactgaac gatcgcatca agaagcgtga gccgtttcgc ccgtttgcgc cgtcgatcct
     1561 ggaagagcgc gtgggtgatt atttcgagca gacgcatccc gcgccgacga tgttgatggt
     1621 ctatcagttc aaacctcaac ggcgcggtga gattccggcg gtgacgcacg tcgatggttc
     1681 gggccgcttg caaacagtct cgcgcgcggt gaacgaacgg tattatcagt tgatttcgga
     1741 tttcgcggcg ttgacgggcg tgccggtagt tctgaatact tcgttcaacg aaaacgagcc
     1801 gattgtttgc acgccgcagc acgcgatcga gtgtttcaga aagacgaaga tggacgtgct
     1861 gtatctcggg aattttgcgg tgcggagaga atagggagag cgataatgcg gattcttgtc
     1921 acgggtggcg cggggttcat tggctcgcat ctttgtgagc ggctcgtcag cgagggaaac
     1981 gaagtgcttt gcctcgataa cttcttcacc ggcaggcgcg agaacatttt tcacctgctc
     2041 gacaatccac gctttgagtt gctgcgccat gacgtcaccg agccgatact cctcgaggtg
     2101 gaccagatct acaacctcgc gtgcccggcg tctccggttc attaccagta caacccggtg
     2161 aagacggtca agaccagcgt gatgggcgca atcaacatgc tcggtctcgc caaacgcgtg
     2221 cgcgctcgca tcctgcaagc ctcgacttcc gaggtttacg gcgacccgct ggtccatcct
     2281 cagaccgaag actactgggg caacgtcaat cccatcggtc tgcgctcgtg ttacgacgaa
     2341 ggtaaacgtc ttgcggaaac gttgatgacc gactatcacc gccagaacaa ggtcgacatc
     2401 cgcatcgcgc gcatcttcaa cacgtatggt ccaagaatgt tggagaacga tggtcgcgta
     2461 gtctcaaact tcatcgtgca ggcattgcgc ggtcagccgt tgacactgta cggcgaaggt
     2521 caacaaacgc gctcgttctg ttacgtcgac gatctgcttg aaggtctgat tcgcttgatg
     2581 aatacggaga accagcacga cccggtgaac ctcggcaatc ccggcgagtt cacgatccgg
     2641 caactggcgg aagaagtgat caaaacgtgc gagtcgaagt cgagcttcac ttatatgccg
     2701 ttgcccgcgg acgatccg
//
LOCUS       DASTEY010000583         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_62535, whole genome shotgun sequence.
ACCESSION   DASTEY010000583 DASTEY010000000
VERSION     DASTEY010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62535"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFO72_10240"
     CDS             complement(<1..807)
                     /locus_tag="VFO72_10240"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479715.1"
                     /translation="MAAFVFCLFLTGTAQTAYASTDKKAYSKGYRAARKGEYPQAEKI
                     FRELLEKDAHDKEARLGLSFVLLKQRNLQAAYDNAARVIMLDPLSARGHALLGSAILG
                     AGEFRLSVEEFRTALALDANEALAVAGLAMVDFYENRLALAINGLRKAVSMDPDEPDY
                     VFSLGQAAARSEKYREAADAYERFLIIAPKTDTDRRERIIGLIAFLRYLGKQGSLYVP
                     AGDDRTTIPFDATDNRPILQVRVNGSKEPLRFVLDTGSGMSVISDETAKRL"
     gene            complement(916..1584)
                     /locus_tag="VFO72_10245"
     CDS             complement(916..1584)
                     /locus_tag="VFO72_10245"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HET9479716.1"
                     /translation="MQRNQEPVTVERVVATHRARRKDIRKRLGEFEEVWRKGSDARLW
                     EELAYCIFTAGASARMGLDSVDAVRPLLLDGEAEAMTAALKEARAHRFPVARPQYIVT
                     TRTYFRSSFGMALRKRLRSFRDPFERRDWLAQEKQVKGLGYKESSHFLRNIGVKGHAI
                     LDKHVMRCLAEVGVVDSAKPPSTRKKYLEVEQNLIRFAKEIRVDFDELDLVLWSMKTG
                     EILK"
     gene            1748..>2791
                     /locus_tag="VFO72_10250"
     CDS             1748..>2791
                     /locus_tag="VFO72_10250"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET9479717.1"
                     /translation="MTTRLKRLLGATILLLVACLTAAAAAAQELPQLVPSPSPSPTPA
                     TESVRPLYGVQGVLVETLDGHIVSSQSENDQFNPASTLKLATALVALKTLGPDHRFAT
                     AVWTDGQFDKATGVINGNLYVSGRDPSFHYERAILVARELNKAGIKQVTGDLFVAPSF
                     TMNFSPSAQRSGEQFYDTLDSTLRRPAAVQAWNYERTLLNDSASLENVPSVAVMGGVG
                     VAAVAPGAKLLLTHKSSRLVDILKVLLCYSNNFMADRIGESLGGPESVRQQLIADLKL
                     MPDDLKISTLSGLGVNRVSPRVMMKIYRALLTELRKHRLGPSAIMPVAGIDPGTLEER
                     FTGPAWRGSVIAKT"
BASE COUNT          595 a          814 c          742 g          640 t
ORIGIN      
        1 caaacgcttc gcagtttcat cggagatcac cgacatcccc gacccggtgt cgagcacaaa
       61 cctcagcggc tccttcgagc cgttcacacg cacttgcagg attggccggt tgtcagtcgc
      121 gtcaaaagga atcgtcgtgc gatcgtcccc ggccggcaca taaagcgatc cctgcttgcc
      181 gaggtaacga agaaaagcga taagaccgat aatccgttcg cggcgatcgg tgtccgtctt
      241 cggcgcgatt attagaaatc tttcgtacgc gtcagcagcc tctctgtact tctcgcttcg
      301 cgcggctgcc tgtcccagac tgaaaacgta atcgggctcg tccggatcca tgctgactgc
      361 tttgcgcaac ccattgatcg cgagtgccag acggttctca taaaagtcga ccatggccag
      421 tcccgcgacg gccagtgcct cgttcgcgtc gagcgcgagt gcagtgcgaa actcctccac
      481 agacaggcga aactcgccgg cgccgagaat cgcggagccc agcaatgcat gaccgcgcgc
      541 tgacaacgga tccagcatga ttactcgtgc ggcattgtcg taggcggcct gcagatttct
      601 ttgtttgagt aagacgaaac tcaaacccag ccgcgcttct ttatcgtgcg catctttctc
      661 gagcagttcg cgaaagatct tctctgcctg cggatactca cctttgcgcg cggcgcggta
      721 tcctttgctg taagcctttt tgtcagtgga cgcataggcc gtttgtgcag tgccggtgag
      781 gaacaaacaa aagacgaaag cggccacact gcaggccgcc gtccggagca gatattgctt
      841 ggtattacat tgcatgatag acaccgttgg acaagcattc ttgcctgttg ttcaggacag
      901 acaggaatgt ctgtcctact tcagaatttc gccggttttc attgaccaca gcactaaatc
      961 gagttcatcg aaatcgactc gaatctcttt cgcgaatcta atcagattct gctcaacttc
     1021 gagatatttt ttccgtgtcg acggtggttt ggcagaatca acgacgccga cttccgccaa
     1081 acagcgcatc acgtgcttat cgagaatcgc atggcccttg acgccgatgt tccgcagaaa
     1141 gtgactgctt tccttgtaac ccaggccctt aacctgcttc tcctgggcca accagtcgcg
     1201 ccgttcaaac gggtcacgga agctacgcaa gcgtttgcgc agcgccattc cgaagcttga
     1261 gcgaaaataa gttcgcgtcg tgacgatgta ctgcggccgc gcgacgggaa aacgatgcgc
     1321 gcgcgcttct ttcagtgccg ccgtcatcgc ttcggcttca ccatcgagta gcaacggtct
     1381 caccgcgtcg acagaatcaa ggcccatgcg cgcggaagcg ccggcagtga aaatgcagta
     1441 tgcaagttct tcccagagcc gcgcgtcaga tcctttacgc caaacttcct caaactcgcc
     1501 gagtcgcttg cgaatatctt ttcgtcgcgc acggtgagta gcaaccactc gttcaactgt
     1561 gacgggttct tgattgcgtt gcactgtagt ttgtcaacag ggcgattcta tgagcgcctt
     1621 cgaaagcttg tcaacgaact cttgaatcat ctgtgtcatc tatgttctgt ggtatcatgg
     1681 gcccgcgggt ccaccgctac ctcacacttt ccccggaaaa tgatttagga gtttgtgtaa
     1741 cgagaaaatg acgactcgat tgaaacggct gcttggtgcg accattttgc tacttgtcgc
     1801 ctgcctgact gctgctgctg ctgctgcaca ggaactgccg caactggttc cgtcgccttc
     1861 cccttcgcct actcccgcaa ctgaatcagt tcgtccgttg tatggcgtcc aaggcgtgct
     1921 tgtcgaaaca ctcgacggtc atattgtgtc atcgcagtcg gaaaacgatc aattcaatcc
     1981 ggcctcgaca ctgaaactcg ctacagcgct agtcgctctt aaaacgctcg gaccggatca
     2041 tcgcttcgcg accgccgtct ggaccgatgg gcagttcgac aaagccactg gcgtcatcaa
     2101 cggaaatctc tacgtttcgg gacgagatcc ttcgtttcac tacgaacgcg ccatattggt
     2161 cgcgcgtgag ttgaataagg cagggatcaa gcaggtcacc ggcgatctgt tcgtcgcgcc
     2221 aagctttacg atgaacttca gcccgtcggc ccaacgatcc ggagaacagt tttacgatac
     2281 tctggactcg acactccggc gccctgcagc cgtgcaggcg tggaactacg agcgcacgtt
     2341 gctgaacgac agcgcgagtt tggaaaacgt gccgagtgtg gccgtgatgg gcggcgttgg
     2401 cgtcgctgcg gtggcccccg gcgccaaact gctcttgacg cacaaatcca gccgactggt
     2461 cgacatactc aaagtgctgc tctgctattc aaataatttc atggcggatc gcatcggcga
     2521 atctcttggc ggtcctgagt cagtgcggca gcagttgatt gccgacctga aactcatgcc
     2581 tgacgatctg aaaatttcga cgctgagcgg gctgggagtg aatcgtgttt cgccgcgcgt
     2641 catgatgaag atctatcgcg cgctgctcac tgaattacga aagcatcgac tcggaccgtc
     2701 ggcgatcatg ccggttgcgg gcatcgatcc gggcacactt gaagaacgtt ttaccggccc
     2761 cgcgtggcgc ggcagtgtca tcgccaagac c
//
LOCUS       DASTEY010000584         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1447426, whole genome shotgun sequence.
ACCESSION   DASTEY010000584 DASTEY010000000
VERSION     DASTEY010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447426"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFO72_10255"
     CDS             <1..277
                     /locus_tag="VFO72_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479718.1"
                     /translation="VVRLLPGALGSETSAVYESFAEGLLDYPQYTRPPEFRGMKVPEV
                     LLSGNHAEIARWRKQAALEKTRRKRPDLLKECTSERGEKEHHNEPIG"
     gene            261..656
                     /gene="rplS"
                     /locus_tag="VFO72_10260"
     CDS             261..656
                     /gene="rplS"
                     /locus_tag="VFO72_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9479719.1"
                     /translation="MNRLDKIEESQLRKNIPEFQPGDTVRVHVRIKESETKERLQAFE
                     GVVIARKHGGARETITVRKTSFGVGVERIFPLHATIIDHIDLVKRGRVRRAKLYYLRG
                     LRGKAARIRERDTRGQQTDDAQAAAAKGK"
     gene            695..1840
                     /locus_tag="VFO72_10265"
     CDS             695..1840
                     /locus_tag="VFO72_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9479720.1"
                     /translation="MRIAIDAHSVGAKLAGNESYAVNLIEALAQVDNVNDYTLYVTTT
                     EAHDRFHDRWPNFKVQTTRPHTPLIRIPLTLSAELRKHPVDVLHVQFTAPPFCPCPVV
                     VSIHDLSFEHLPQTFHRRSRTQLRLTVRHSARRADRILTLSEHTRGDIIETYRIDPEK
                     ITAIPLAAPAHFGPIADDKELQRVRHTYGIDRDYVLSVGSIQPRKNLVRLINAYASLR
                     GVHTGNSFPKLVIVGKRAWLYDETLRALEETGVRDSVVLTGYVPESDLPPLYSGALCF
                     VYPSYFEGFGLPPLEAMKCGAPVIVGNATSLPEVVGDAGLQVDPFDVSAIAAAMDRLI
                     NYPELRTQLSAKGQKRAEMFDWRETARQTLKVYEQAGRKCTDQQQVN"
     gene            1844..3001
                     /locus_tag="VFO72_10270"
     CDS             1844..3001
                     /locus_tag="VFO72_10270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9479721.1"
                     /translation="MRIAILGTRGIPASYGGFETFAEHLSTRLVARGHDVTVYCRAHY
                     VSPRQLEYHGVRLEVLPTIRHKYFDTVVHAFLSAVHAVSRRFDAVLICNSANAPFSPI
                     LRFTGTPVAINVDGLEHKRKKWGWLGRRYYRLAEYLSTVLPNEVVTDAQVIQDYYQVH
                     YNTSSTMIAYGSEVERRPDRETVRKWRVEPNRYVLYVSRLEPENNAHLVIEAFKKVRT
                     AYRLLIVGDAPYAESYINSLKARARGDKRIIFTGFVFGQDYRALQQNAYCYVHATEVG
                     GTHPALLEAMGYGNCVLTLATPENIEVVGDAGVPYLDEFDLAEKLQRVLRDGSLVHAY
                     RHRAQLRIRNHYDWETVVDQYERLFARMCGQDLPEHRKVAEPAASEVSAGV"
BASE COUNT          719 a          831 c          888 g          653 t
ORIGIN      
        1 ggtggttcgt ctgttacccg gcgcgttggg tagcgagacc tcagcagtgt atgagtcgtt
       61 tgccgaaggc ttgcttgact atccacagta cacgcgaccg ccagagtttc gcggcatgaa
      121 agtgccggaa gttttattga gcggcaatca cgcggagatc gctcggtggc gaaaacaggc
      181 cgcactggaa aagactcggc gcaaacgtcc ggatttatta aaagagtgca caagtgaacg
      241 cggagagaag gagcatcaca atgaaccgat tggataagat tgaagagagc cagttacgga
      301 agaatattcc ggagtttcag ccgggcgata cggtccgcgt tcatgtccgg atcaaagaat
      361 ccgagaccaa agagagattg caggcttttg agggcgttgt aattgcgcgc aagcacggtg
      421 gcgcgagaga gacgattacg gtgcgcaaaa ccagcttcgg tgtgggcgtc gaaagaatct
      481 ttccgttgca cgccacgatc atcgaccata tcgatctcgt taaacgagga cgcgtgcggc
      541 gagcaaagct ctactacctg cgcggacttc gaggtaaagc ggcacgtatt cgcgagcgcg
      601 atacgcgcgg ccagcaaacc gacgacgcgc aggccgcggc agccaaaggc aagtaaactt
      661 tctcggttgc gagaagtgaa acgggagaaa ccgattgcgg atcgcaatcg atgcgcattc
      721 tgtcggggcg aagcttgcgg gaaatgaaag ctacgccgtt aaccttatcg aagcgctcgc
      781 gcaggtcgat aacgtcaacg attacactct ctacgtcaca actactgaag ctcacgatcg
      841 tttccatgac cgctggccca atttcaaagt tcagaccacg cggcctcaca cgccgctaat
      901 cagaatcccg ctgacactca gtgccgaact ccggaagcat ccggtcgacg tgctgcacgt
      961 gcagtttaca gcgccgccgt tttgtccgtg cccggtcgta gtcagcattc acgatctttc
     1021 gttcgaacat ctgccgcaaa cttttcatcg tcgcagccgc acgcaattgc gcttgactgt
     1081 gcggcattct gcgcgtcgcg cagacaggat cctgacgctc tccgagcaca cgcgcggaga
     1141 tatcatcgag acctaccgca tcgatcccga aaagatcact gcgattccgc tggcagctcc
     1201 ggcccacttt ggtccgatag ctgacgataa ggaacttcaa cgtgtgcgac acacttatgg
     1261 gatagaccgc gactacgttc tgtcggtcgg ttccattcag ccccgcaaaa atctggtgcg
     1321 tttgattaat gcgtacgcat cgttgcgagg cgttcacaca ggaaatagtt ttccaaaact
     1381 cgtcatagta ggtaaacgcg cctggcttta cgatgagact ttgcgtgcac tggaagaaac
     1441 gggcgtcaga gactcggtgg tgctgaccgg gtacgttccc gaatcggatc taccgccact
     1501 ctactcggga gcgctctgtt ttgtttaccc gtcgtacttt gaaggttttg gtttaccacc
     1561 actcgaggca atgaagtgcg gcgctccggt gatcgttgga aacgccacaa gcctgccgga
     1621 agtcgttggt gatgctggtt tgcaggtgga tccgttcgac gtttccgcga tagccgcagc
     1681 tatggaccgg ttgataaatt atccggaact acgcacgcaa ctaagtgcga aaggtcaaaa
     1741 gcgcgccgag atgtttgact ggcgcgagac ggcccggcaa acgttgaaag tctacgaaca
     1801 ggccgggcgg aaatgtaccg atcagcagca agttaactag agaatgcgca tcgcaatcct
     1861 gggaacgcga ggtattcctg ccagctacgg tgggtttgaa accttcgccg agcacctttc
     1921 cacacgactt gtggctcgcg gccacgatgt gactgtgtac tgccgcgcgc actacgtgtc
     1981 accgcgacag ttggaatatc acggcgtacg gctcgaggtg ttgccgacca tccggcacaa
     2041 atacttcgat accgtggtcc atgcttttct atccgcggtt cacgcagtat cacggcgttt
     2101 tgacgctgtt ttgatctgca actctgcgaa cgctcctttc agtccgattc tccggtttac
     2161 cgggacgcca gtcgcaatca acgtcgacgg actcgaacac aagcgcaaaa aatggggctg
     2221 gctgggccgg cggtactatc gactcgccga gtatctttcg acggtgttgc cgaatgaagt
     2281 ggtaaccgac gcgcaggtta ttcaggatta ctaccaggtc cattacaata cttcgtcaac
     2341 catgattgcc tatggcagcg aagtcgaacg gcggccggat cgggaaacgg ttcgaaaatg
     2401 gcgcgtcgag ccgaatcgct acgtgctcta tgtctcgcga ctggagcccg agaacaatgc
     2461 tcaccttgta atcgaagctt ttaagaaggt gcggaccgct tatcggctgt tgatcgtcgg
     2521 ggatgcgcct tacgccgaaa gttatatcaa cagcttgaag gcccgggcgc gcggcgacaa
     2581 acgaatcatc ttcacgggct ttgtttttgg tcaggattat cgggcgctac aacaaaacgc
     2641 ctattgttac gtccacgcaa cagaggtcgg cggcacgcat ccggcgctgc tcgaggccat
     2701 gggttacggc aattgcgtgt tgacacttgc cacaccggaa aacatcgagg tagtcggcga
     2761 cgcgggcgtg ccgtacctcg atgagtttga tctggccgaa aaactccagc gtgtgctgcg
     2821 cgacggctcg ttggtccatg cgtaccgtca ccgcgctcaa cttcgcattc gcaaccatta
     2881 cgactgggaa acggttgtgg accagtacga gcggctgttt gctcgtatgt gcgggcaaga
     2941 tcttcccgaa cacaggaaag tcgccgaacc ggccgcatcc gaagtttcgg cgggtgttta
     3001 gcgaagaaaa aagatccaga aagagaggtg ccccgcctgt tcacaggaac tggcggggca
     3061 aagcgttaaa gggcagggtt cgggcaaatc a
//
LOCUS       DASTEY010000585         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1182957, whole genome shotgun sequence.
ACCESSION   DASTEY010000585 DASTEY010000000
VERSION     DASTEY010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182957"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFO72_10275"
     CDS             <1..1184
                     /locus_tag="VFO72_10275"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET9479722.1"
                     /translation="HTHLREPGHEYKETVSSGAAAAVAGGWASICAMPNTDPVNDNPA
                     VTRFIIEQAQAAKLANVFPIGAITKGSNGKELAEMGEMKNAGIVAVSDDGRPVPTSGM
                     MRRAMEYARGFDLPVVDHCQDPSLSAGGVMHEGRWSLILGLRGMPAAAEEVDAIRDCV
                     LAKLTGAKVHIAHVSTRGALEAVRQAKEQGAQVTCEVAPHHWTLTDEAVAEYDTNTKM
                     SPPLRSRDHLDAILAAMKDGTIDAIATDHAPHHQDEKALEFDQAPFGIVGLETAVGLA
                     FELVHQGLIDLQRVVEMCSTNPARIFGLAERGSLKQGSHADVTILDPQHRWAFEVKRS
                     KSKSRNTPFDGRAMHGAAVATIVGGRIVYLHPDYTRLIEPTLGPQASRLHRTASGAIV
                     K"
     gene            1484..2110
                     /locus_tag="VFO72_10280"
     CDS             1484..2110
                     /locus_tag="VFO72_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9479723.1"
                     /translation="MEFTKKEQNALEHAISVLFDVGPNGYATGFLVGNGLVMTAYHVI
                     SGNLSGTKKVMLGFRASDELSVKVFVDGCRAKVLKVDKEADLALLEMCRNSKDAKAPT
                     FQTAPVKDDKLFLIARPHGDKVVSYGSFYGNYMLGNQEYWSVKIDSRDGFSGSPVYNS
                     KGEVVGVFSGYDWSQKLALISPSIRAQKLLEEYHEESSAAAAAKPTQP"
     gene            complement(2261..3229)
                     /locus_tag="VFO72_10285"
     CDS             complement(2261..3229)
                     /locus_tag="VFO72_10285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9479724.1"
                     /translation="MSTDLSIIIVNWNGGELLRRCVETIVSSEPRVTYEVVIIDNASS
                     DDSLDQLKAGEAAKSLTAHQQLRIFNNSENRGFGAANNQAFDLTDSPFVFLLNLDAEV
                     HPGTIDTLMRTMLSDPKIGACGPKILTTDGSLQISAFFNPPRVWHTVLSQLKLYYLLP
                     QRIRGELLLGRHWDHNRQRSVPMLSGAAILARRQMIEEVGGFDERFHMYAEDNEWCWR
                     ITRTDWKLVFVPEAVVLHHGGQSSMKRWSSDEKLRVKLEAGFYFEHRVLPRWRLAANQ
                     LANYLVVSAQIAGRQMRGIHIPELYLIKEMHREHLKRSLRNHLERK"
     gene            complement(3226..4209)
                     /locus_tag="VFO72_10290"
     CDS             complement(3226..4209)
                     /locus_tag="VFO72_10290"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9479725.1"
                     /translation="MRISVAMCTYNGADFLPAQLASIAEQTRPPDEIIVCDDASTDHT
                     RAQLESFARTSPIPVSLYFSDQNAGSLKNFERAIGLCTGEVIALSDQDDVWRNDKLQV
                     IEQTFRRHPITGLVFSDAEIVDKNLKPLGQRMWNEVGFDARKQKLMRNGRSLDVLVTG
                     WTVTGATMAFRSEYVKLALPIPANIAMIHDGWIALTIAAVADVAFIEEPLIKYRQHER
                     QQIGVPNRPVVQPERGLQAFESALRRRNSARDLHGILAALEERLIAQQNSFDCRRALA
                     FAGNYSLHLDVRANLPRKRLSRVPRILRELLTMRYHEYANGFKSAAKDLVS"
BASE COUNT          948 a         1201 c         1258 g          929 t
ORIGIN      
        1 tacacacgca cctgcgcgag ccgggacacg aatacaaaga gacggtctca tccggcgccg
       61 cggcggctgt cgcgggtggg tgggcgagca tttgtgcgat gccgaacaca gatcccgtta
      121 acgataatcc cgctgtcacg cgtttcatca tcgaacaagc acaggcagca aaactcgcca
      181 acgtcttccc catcggtgca atcacaaaag gcagcaacgg caaagaactc gcggaaatgg
      241 gtgagatgaa gaacgcggga attgttgccg tgtcggatga cgggcggccg gtgccaacgt
      301 caggaatgat gcgccgcgcg atggaatatg cgcgcgggtt tgatcttccc gtagtagatc
      361 actgtcagga tccgtcgctc agcgccggcg gcgtgatgca cgaaggccgt tggtcgctga
      421 ttttgggcct ccgcggaatg cctgcggctg cggaagaggt cgacgcgatt cgcgactgcg
      481 tgctcgctaa actaaccggc gcgaaggttc atattgccca cgtctcgact cgcggcgcgc
      541 tcgaagcagt gcggcaggcg aaagaacaag gtgcgcaagt cacttgcgaa gtcgcgccgc
      601 atcattggac gttaacggac gaagcggtcg ctgagtacga cacaaacacg aaaatgagcc
      661 cgccgttacg cagccgcgat catctggacg cgattctggc ggcgatgaaa gacggcacca
      721 tcgacgcgat tgccacagat cacgcgccgc atcaccagga tgaaaaggct ttggaattcg
      781 accaggcgcc gtttggcatt gtcggcctcg aaactgctgt tggtctggcg tttgagctgg
      841 ttcaccaggg tttgatcgat ttgcagcggg tcgttgaaat gtgctctaca aatccggcgc
      901 gaatctttgg tctggccgaa cgcggttctt tgaaacaggg ctctcatgct gacgtgacca
      961 ttctcgatcc gcaacaccgc tgggcctttg aggtcaaacg ttcaaagtcg aaaagccgca
     1021 acactccgtt cgacggacgg gccatgcatg gcgccgcagt cgcgaccatc gtgggcggta
     1081 gaatcgtcta tctccatcct gactacactc gcctcattga accaacgctg ggaccgcagg
     1141 cgtctcgcct gcacaggact gcctcgggcg cgattgtgaa atagcagacg agacgcctgc
     1201 gatccgagta cgggcgcaca tggatcgaag aaataaatcg cacaattccg acaacacaaa
     1261 atcattgcca atccggagac acggccctaa aatgctccgg tctcggttcc caataatatt
     1321 cctagacccc ttcaacaccg cggaggtcac gatgaagagt aagctttgca gccttactag
     1381 tatggcggcg ctcatcgttt tgctggtcgc tcctgtgtgc gtagctgaca aaagctccgc
     1441 caggctgctg aagcaacaag agcgctatac cctgtcgctg gaattggaat ttacaaagaa
     1501 agagcaaaat gcgctggagc atgcaatctc agttctgttt gatgtcggtc ctaatggata
     1561 tgcgacaggg ttcctggtag gcaatggact ggtgatgacc gcctatcacg tgatctcggg
     1621 aaacctgagc ggcaccaaaa aggtcatgct tggtttccgc gcctccgacg agctgagcgt
     1681 taaggtcttt gttgacggct gccgggccaa agttctcaaa gtggacaaag aagccgatct
     1741 cgcgttgctc gagatgtgcc gcaactcgaa agacgccaag gctcccactt ttcaaaccgc
     1801 gcccgttaaa gatgacaaac tctttctcat cgccagacca cacggcgaca aagtggtgag
     1861 ttacggtagc ttttacggaa actacatgct gggcaatcag gagtactggt cggtgaagat
     1921 cgacagccgc gatggtttct cgggcagtcc ggtttacaac tcgaaaggcg aagttgtagg
     1981 cgtgttcagt ggttacgact ggtcgcagaa gctggcgtta atcagcccca gtattcgagc
     2041 gcagaaactg ctcgaggaat atcacgagga gtccagcgct gctgccgccg caaagccaac
     2101 gcagccttag ctgggtaggg actgtcttga atcccaggac cttgaattcc ggcaccgcag
     2161 acgtctcgcc tgcaactcac ggcagcgttc gctgcgctac catgcaggcg agacgcctgc
     2221 ggtcccaggt cagaaggttc tcaaggcgta cggctttgct ttatttgcgt tccaggtggt
     2281 ttctgagcga gcgcttcagg tgctcgcgat gcatttcctt gatcagatag agttcaggga
     2341 tgtgtatgcc gcgcatttgc cgcccggcga tttgggctga tacgaccagg tagtttgcta
     2401 gttggttggc agcgagtcgc cagcgcggta gtacgcgatg ttcgaagtaa aatcctgcct
     2461 ccagttttac gcgcaacttc tcatctgagg accaacgctt catcgaactc tgcccgccgt
     2521 gatggaggac gactgcttcc gggacgaata ccagcttcca gtcggtccgc gtgatgcgcc
     2581 agcaccactc gttgtcctcg gcgtacatat ggaaccgttc gtcgaagccg cccacttctt
     2641 caatcatctg ccggcgggcc aggatcgctg cgccgctgag catcggcacc gatcgctgac
     2701 gattatggtc ccaatgtcgg cccaaaagca gttcgccgcg aatgcgttgc ggcagcaggt
     2761 aatagagttt taactgcgaa agcaccgtgt gccacacgcg cggcgggttg aagaatgcgc
     2821 tgatttgcag cgagccgtcg gtggtgagaa tctttggccc acaagcgccg atcttcgggt
     2881 cagacagcat tgtccgcatt aaggtgtcga tcgttcccgg atgcacttcc gcgtcgaggt
     2941 tgaggaggaa gacgaacggc gagtcggtta gatcgaaggc ctgattattc gcggcgccga
     3001 agccacggtt ttcagagttg ttgaagatgc gtaactgctg gtgggccgtt aacgatttcg
     3061 ccgcctcgcc cgccttcaat tggtccaggc tgtcgtcgct ggaggcgttg tcgataatca
     3121 cgacttcgta ggtgacccga ggctcggagc tgacgatggt ttccacgcac cgccgcaaaa
     3181 gttcgccgcc attccagttg acgatgatga tcgagagatc agtactcaag agaccaggtc
     3241 cttagcggcg ctcttaaaac catttgcata ctcgtgataa cgcatcgtca gcagttcgcg
     3301 caatatcctg ggcacgcggc tgagacgctt ccgcggcaag ttcgcgcgaa catcgaggtg
     3361 gagcgaatag tttccggcaa acgcgagtgc gcgacggcag tcgaacgaat tctgctgcgc
     3421 gatcaggcgt tcctccaacg ccgcgagtat tccgtgcaaa tcacgcgctg aattacgccg
     3481 gcgcaaagct gattcgaaag cttgtaagcc acgttccggt tgcacgacag gacgattcgg
     3541 cacgccgatc tgctgtcgtt cgtgctggcg atatttgatt aatggttctt caataaaagc
     3601 aacgtccgcg accgccgcaa tcgtcagcgc gatccagccg tcgtgaatca tggcgatgtt
     3661 tgccggtata ggcaacgcca gtttcacata ttcggaacga aaagccatcg tggccccggt
     3721 taccgtccaa cccgttacca gcacgtcgag cgatcttcca ttgcgcatca gtttttgttt
     3781 gcgggcgtca aacccgactt cgttccacat tcgttggccc agcggcttca ggtttttgtc
     3841 gacgatctcg gcgtccgaga agacgagtcc cgtaattgga tggcggcgaa acgtctgctc
     3901 gataacctgc aatttgtcgt tgcgccagac gtcgtcctga tcgctcaaag cgattacctc
     3961 gccggtgcac aagccaatgg cgcgttcgaa gtttttaagc gagccggcgt tctggtccga
     4021 gaaatacagt gatacgggta tcggagacgt tcttgcgaac gactccagct gcgcacgtgt
     4081 atggtcggtc gaagcatcgt cgcaaacgat gatttcatca ggcgggcgcg tctgttcggc
     4141 gatgcttgcc aactgcgcgg gtagaaaatc ggcgccgttg taagtacaca tcgccacaga
     4201 gattcgcata cgcggcattc tacatctccg cctaaatcgt ggctaatagc tcacaggtgt
     4261 agcttgaggc gtctcgtttc ctgggaccgc aggcgtctcg cctgcatagt agcgcatcgg
     4321 gcgctgccgt aagttg
//
LOCUS       DASTEY010000586         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1216302, whole genome shotgun sequence.
ACCESSION   DASTEY010000586 DASTEY010000000
VERSION     DASTEY010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216302"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..735)
                     /locus_tag="VFO72_10295"
     CDS             complement(172..735)
                     /locus_tag="VFO72_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9479726.1"
                     /translation="MTPPESPDEMLVIAAILGNLEAFEQLVVRYRPAVVRLARSIVGA
                     DYAEDVTQDSLLLAFKALPSIEEPRKFAAWLSAITRHRALRFSQSETAHTNRRVALDE
                     ALLQTIEALAKPLAERALAERERDESMVKAIDSLPVEYAMPLKLRFLDEMPLNRIAAF
                     MGVPLSTVKWRIHHGKKLLRAKLEPMA"
     gene            865..3507
                     /locus_tag="VFO72_10300"
     CDS             865..3507
                     /locus_tag="VFO72_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9479727.1"
                     /translation="MRQVDTSLKSYALGFVAVVLAVLLRWMLDPLLGDAFPLVTLYGA
                     VAAAVWLGGFRVAIPASLLGYIAIHFLFIPPRGVFDLNDRANLVGLAAYLFTCSLIIG
                     FGEAARIAHIRALERREILRVTLQSIGDAVITTDMDGRVTGLNKVAEALTGWSLNEAR
                     GQPLERVFRIVNEVTRQSVENPATRSLREGVVVGLANHTVLIKKDGTECPIDDSAAPI
                     RDEQGHISGCVLTFRDVTAQRLMERDRVNQLHTARLLASIVESSNDAIIGKSLDRSIQ
                     TWNAAAERLFGFTAEQAIGRDISIVIPPDRLAEEDQIIAKLKAGERIEHFETERVRCD
                     GQRIIVSLTISPIKDEAGNVIGASKIVRDVTERKRVERERENFVAVIENSTDFIGMCD
                     LNGFPFFINRAGLENVGLDSMEEARRTPVVEFFFPEDQPKIMNELFPQVLETGHGELE
                     VRFRHFKTGEARWMVYKVLKLTDADNRPVGFATVSQDITERKQLEDNLRVLADGLSEN
                     DRRKNEFLATLAHELRNPLAPMSNMLEVVKRANGDSEILKRAYETIERQLGQMVRLVD
                     DLLDLNRITHDRLELRRSEVELSSVVQQAVEVARPLMDAAGHQLTIELPDEPIYLNAD
                     RARLAQVFGNLLNNSCKYTKAKGVISLSGKRIGDEVVVTVRDNGAGIPPDKLDSIFDM
                     FMQVDRTSHQSQGGLGIGLTLVKRLVDMHGGSIEAKSDGEGRGSEFVVRLPVLSAPSV
                     VSEVGTDGAQEEPTQRRILIVDDNRDSADSLAMLLEITNNKTYMAHDGVEALEAIEKN
                     RPEVVLLDIGLPKLDGHEVCRRVREQPWGKDVVIIALTGWGQEDDRRKSEEAGFNGHL
                     VKPVDYDQLLELLRELTSGSQTRV"
BASE COUNT          825 a          941 c          991 g          764 t
ORIGIN      
        1 gagtcgagct ccttcagacg cgacagcacg cgattgtact ggtcggtgca gaacgcagcc
       61 gcgtcttctt tgccgccaat ccactccgac tgcagcgcca tgcgcgacgt gcgtcgcagc
      121 acattgatca acttaaccaa ctctttctct cgttccatct ggttctcctc gctaagccat
      181 tggttctaat ttggcgcgta acaatttctt gccgtgatgt atcctccatt tgaccgtcga
      241 caacggcaca cccataaacg cagcgattct gttcagcggc atctcgtcga gaaaacgcaa
      301 cttcaaaggc atcgcatact cgacaggaag actgtcgatc gccttgacca tcgactcgtc
      361 tctttctcgt tcggccagcg ctcgctcggc gagcggcttc gccagcgctt cgatcgtttg
      421 cagtaaagct tcatccaaag cgacgcgtct gttcgtgtgc gcagtttcac tctggctgaa
      481 ccgcagcgct cgatggcgcg ttatggccga caaccaggcg gcaaacttac gcggttcctc
      541 gatcgagggc agcgccttaa aagcgagcag aagcgagtcc tgcgtcacgt cttccgcgta
      601 atcggcgccg acaatacttc gcgccagcct gacaaccgcc ggccggtacc gcaccacgag
      661 ctgttcaaat gcctccagat tgccgagaat cgccgcgatc acgagcattt cgtcaggcga
      721 ttcaggcggt gtcatccaga gctccggttc aacacgactc gtctgttggt atagagacat
      781 gttacgacgg aaaggttagc cgagttttca attaaaggat aaaattggaa gtcccgaata
      841 actctataaa gtaagtcgcc atacgtgaga caagtagaca cgtcactcaa atcatacgcg
      901 ctcggattcg tggcagtggt actggcggtg ctgctgcgct ggatgctcga tcccttactg
      961 ggcgacgcgt ttccgctggt cacgctttac ggcgcagttg ccgcggccgt gtggctcggc
     1021 ggatttcggg tggcaatccc ggccagtctg ctcggctata ttgcaatcca cttcctgttc
     1081 attcccccgc gaggcgtatt tgatttaaac gaccgtgcaa acctggtcgg attagccgca
     1141 tatctcttca cttgttctct aattattggt ttcggcgagg cggcgcgcat cgctcatata
     1201 agagcgcttg agcgtcgcga aatcttgaga gtcacgctgc aaagcattgg cgacgcggtg
     1261 attactaccg acatggatgg acgcgtcacg ggcctcaaca aagttgccga ggcattaaca
     1321 ggctggtcac tcaatgaggc gcggggccag ccgctcgagc gcgtgtttcg gatcgtcaac
     1381 gaggtcactc gtcagagtgt cgaaaatccg gcgacacgat cgttgcgtga aggcgtggtt
     1441 gtcggtctcg caaatcacac ggtattgatt aaaaaggacg ggaccgagtg cccaatcgac
     1501 gatagcgcgg cgcccattcg cgacgagcag ggacacattt caggatgcgt attgaccttt
     1561 cgcgatgtta cggcccaacg cctgatggag cgtgataggg taaaccaact tcatacggcg
     1621 cgccttctcg catcaatcgt cgagtcttca aacgacgcga ttatcggtaa atcactcgac
     1681 cgctccatcc aaacctggaa cgctgccgcg gaacgactct tcggtttcac tgcggaacaa
     1741 gcgatcggcc gcgacatctc aatcgtgatc ccgcctgacc gcctggccga ggaagatcaa
     1801 ataatcgcca aattaaaagc tggagaacga atcgagcact ttgagacaga gcgagtgcgc
     1861 tgtgacggac agcgcatcat tgtttcactc acgatatcgc caatcaaaga cgaggccggc
     1921 aacgtgattg gtgcttccaa gatcgtgcgc gatgttaccg agcgtaagcg cgtcgaacgc
     1981 gagcgggaaa acttcgtagc agtcattgaa aacagcaccg acttcatcgg catgtgtgac
     2041 ctgaacggtt ttccgttctt catcaatcgc gctggattgg aaaacgtcgg attagacagc
     2101 atggaagagg ctcgccgcac gccggttgtt gaattcttct tccccgaaga tcaaccaaag
     2161 atcatgaatg agctgtttcc acaggtgctg gaaacaggtc atggtgagct cgaggttcgc
     2221 ttccgccatt tcaaaaccgg tgaagctcgc tggatggtct acaaagttct gaagctaact
     2281 gacgctgaca atcggccggt ggggtttgcg accgtcagcc aggacattac cgaacgaaaa
     2341 caactggaag acaatctgcg cgtattggct gatggtctgt ctgaaaacga tcgccgaaag
     2401 aacgagtttc tggcgacgct tgcacacgag ctcaggaacc cgttggcgcc tatgagcaac
     2461 atgcttgaag ttgtgaagcg tgcaaacggc gacagcgaaa ttctcaaacg agcgtatgaa
     2521 accattgaac gccagttggg ccagatggtg cggctggtgg acgatctgct cgatttgaat
     2581 cgcatcactc acgaccgtct cgagttgcga cgcagcgaag tcgaactatc ttcggtggtc
     2641 caacaagccg tcgaagtcgc gcgtcccctg atggacgccg cgggccatca gctaacaatc
     2701 gaattaccgg atgagccgat ctatctgaac gccgatcgcg ctcgattggc ccaggtgttt
     2761 ggcaacctgc tcaataacag ttgcaaatac actaaggcga aaggagtcat ctcgctctcg
     2821 ggaaaacgaa ttggcgatga ggtcgttgtt acagtcaggg acaatggcgc cggaataccg
     2881 ccggataagc tcgacagtat tttcgacatg ttcatgcaag tcgatcgcac ttcgcatcaa
     2941 tctcagggcg gactcggaat cggcctgacg ctcgtaaagc gtttggtcga tatgcatggt
     3001 ggatcgatcg aagcgaagag tgatggtgaa ggaaggggaa gtgagtttgt ggtgcgtctg
     3061 ccggttctga gtgcaccttc agtcgtgtct gaagttggaa cggacggcgc acaagaagaa
     3121 ccaacgcagc gccgcattct gattgttgat gacaacagag actctgcgga ttcccttgcg
     3181 atgttgttag agatcacgaa caacaaaacc tacatggcgc atgatggcgt tgaggcactc
     3241 gaggcgattg agaagaatcg accggaagtg gtgctgctgg acattggctt gccgaaactg
     3301 gacggacatg aggtctgccg gcgcgttcgc gagcagccat ggggcaagga cgtcgtgatc
     3361 atcgcgctga ccggctgggg ccaggaagac gatcgacgca agtcagaaga agccggcttc
     3421 aacgggcact tagtgaagcc ggtggattac gaccagttac tggaactgtt gcgcgagctg
     3481 acgagtggct cgcaaacccg ggtttaagag tgagctttta t
//
LOCUS       DASTEY010000587         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_359229, whole genome shotgun sequence.
ACCESSION   DASTEY010000587 DASTEY010000000
VERSION     DASTEY010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359229"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFO72_10305"
     CDS             <1..964
                     /locus_tag="VFO72_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479728.1"
                     /translation="REKHPNEPSGTTPGWFFIAIVGVFLLASPAVLMPEVWHYMNAYM
                     GEQLLTHSGYLFAGQLYKNNMSSSPFWGTPIYFYLLFLAIKVPLMTLLAFLIGLFVAI
                     RRPRHVGHAFLLFMFLFWLVPYSLIGGKWLRYTLSVMPFVYMFAAVGVVALVRWTSRR
                     FPDSRRALLSFSAIAVVMFVCFPAWTAFASRPHYALYTNALAPGKAGYFFPHDEFYDD
                     GLREAIKFVCDQAPQGATIAHETPGVTRYYLSLFERKDLNSQVISSAEFDVTSAPGPV
                     YVIVQRGRTYFENRDELQAVRAKFRKVHAVTIDGVIAVEVFVNQ"
     gene            961..>2194
                     /locus_tag="VFO72_10310"
     CDS             961..>2194
                     /locus_tag="VFO72_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9479729.1"
                     /translation="MKRRSPFFHSFITLVMMLTLVLVARSFVPDRVQITILGTTDLHG
                     NINPIDYYTNKPDNRGLAKIATLVKRIRKEHPNVLLVDSGDTIQGSPLASFHSRKNNQ
                     PPDPMMLVMNSLNYDAMAVGNHEYNFGLKVLEKARAEANFPWLSANTYNIAKQQPHYK
                     PYIVKEVAGVRIGILGLTTPGVPNWDNPSNYAGLEFHNPVSEARKWVPILRDKEKVDV
                     VVVAMHMGLGEDLRTGEVNPGQVPHENEAISIAKEVPGVDLIFMGHTHREVPSVYING
                     VLLTQANHWGRHLARADVYLQKSTTGGWRVYAKAARTLPADDRVEPDPEVTKLAEPYD
                     NETQGWLAKVIGQSAEELTAKDARFRDTAILDLIQKVQLEAGKADVSMVASFNPEARI
                     AKGPVSVRDIAGLYVYENT"
BASE COUNT          477 a          606 c          619 g          492 t
ORIGIN      
        1 gcgtgaaaag catccgaacg agccgagcgg aacgacgccc ggttggttct tcatcgcaat
       61 cgtgggtgtg ttcttgttgg ccagtccagc agtgttgatg ccggaagtgt ggcactacat
      121 gaacgcgtac atgggcgaac aactgttaac gcactccggt tacttgtttg cgggacagct
      181 ttataaaaac aacatgtcga gttcgccgtt ttggggaacg ccgatctatt tttacttgct
      241 gtttctggcg atcaaagttc cgctgatgac actgctcgca tttctgatcg gtttgttcgt
      301 ggcgatcaga cgcccgcgcc acgtcggtca cgcgtttttg ctgttcatgt ttctgttctg
      361 gctcgttcct tactcgctga tcggcggcaa gtggttgcga tacacgcttt cggtgatgcc
      421 gtttgtttac atgttcgcgg ccgttggtgt ggtggcgctg gtgcgatgga ccagcagacg
      481 atttccggat tcgcggcgcg cgttgttatc tttctcagcg atcgcggtcg tgatgtttgt
      541 ctgttttccc gcctggactg cctttgcgag ccggccgcat tacgcgcttt ataccaacgc
      601 gctcgctccc ggtaaagcag gttacttctt cccgcatgac gagttttacg acgacggact
      661 acgcgaagcg atcaagtttg tttgcgacca ggcgcctcag ggagcaacca ttgctcacga
      721 aacaccgggc gtcacgcgct actatctttc cctattcgag cgcaaggatt tgaactcaca
      781 ggtgatctcg tcagccgagt tcgatgtgac ttcagcgccg ggtccggttt acgttatcgt
      841 gcagcgcggc cgcacttatt tcgaaaaccg cgacgagctg caagccgtac gcgcgaagtt
      901 tcgaaaagta catgcagtta ctatcgacgg tgtcatagcg gttgaggtat ttgtaaatca
      961 atgaagagac gttcaccgtt tttccactcg ttcataaccc tggtcatgat gctgacgctc
     1021 gtgctcgtcg cacgaagctt cgtgcccgac cgcgtccaga tcacgatcct cggcactacc
     1081 gatctgcacg gcaacatcaa ccccatcgat tactacacaa acaagcctga caatcgcgga
     1141 ctcgcaaaaa tcgcgacgct cgtcaaacgc attcgcaagg aacatccgaa cgttctgctc
     1201 gtcgactccg gcgacacgat tcagggctcg ccgctcgcgt cgttccatag ccgtaagaac
     1261 aatcagccgc ccgatcccat gatgctggtg atgaactcgt tgaactacga cgcaatggcc
     1321 gtcggcaatc atgagtacaa cttcggtctg aaggtgttgg agaaggcgcg cgccgaagcg
     1381 aactttccgt ggctctcggc gaatacatac aacatcgcga agcagcagcc gcactacaag
     1441 ccttacatcg tcaaggaagt cgcgggcgta agaatcggaa tactcggtct gacgacgccc
     1501 ggagtgccga actgggacaa cccgtcaaat tacgccgggc tcgagtttca caaccctgta
     1561 agcgaagcgc gcaagtgggt tcccatcttg cgtgacaaag aaaaagtcga cgtggttgtc
     1621 gtcgcgatgc acatgggcct cggcgaagat ttgcgcaccg gcgaagtgaa tcccggacaa
     1681 gtgccacacg agaatgaagc gatttcaatc gcgaaagaag ttcccggcgt cgacctgatc
     1741 ttcatgggcc acacgcatcg cgaggtgcct tcggtctaca tcaacggcgt tctgctcacg
     1801 caggcaaacc attggggcag gcatctcgcg cgtgcggatg tttacttgca aaagtccacg
     1861 accggtggct ggcgcgttta tgcgaaagcc gcgcgtacgt tgccggcgga cgaccgcgtc
     1921 gaacccgatc cggaagtcac caaacttgcg gaaccttacg acaacgaaac gcaaggttgg
     1981 ctggcaaagg ttattggcca gtcggcggaa gagctgacgg cgaaggacgc gaggttccgc
     2041 gataccgcga ttctcgatct gattcagaaa gtacagctcg aggctgggaa agccgacgtg
     2101 tcgatggtcg cgagtttcaa tcccgaagcg aggatcgcga aagggccggt gagtgtgcgc
     2161 gacatcgctg gtctgtacgt ctatgaaaac acgc
//
LOCUS       DASTEY010000588         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1101342, whole genome shotgun sequence.
ACCESSION   DASTEY010000588 DASTEY010000000
VERSION     DASTEY010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101342"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFO72_10315"
     CDS             <1..848
                     /locus_tag="VFO72_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479730.1"
                     /translation="STIQSVMPSRFASRVQIIVRGDQDIVEDDFSKDSETELSHDDLE
                     AAHSPAPVVHIGNREGALLVDYAYGAGRIVVLSDPYIVTNSGIKLNDNLQLAINALGG
                     GRGLIAFDEYHQGRGVTQNAFASYFAGTPVLALAAQIVLVILLILWTNARRFGRPLPL
                     PHVDRRSSLEFVASMAELQERSRAFDLAIENIYTRTRRVLARYAGMDYNSSRSEIATR
                     VAARSNIDGQKLETLMRQCEEAINGAPISWRQSLDLVRRLREVERDLGLRMRSRDVRQ
                     AAENI"
     gene            881..1834
                     /locus_tag="VFO72_10320"
     CDS             881..1834
                     /locus_tag="VFO72_10320"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9479731.1"
                     /translation="MPETVASTVEHIRTELRKVIVGQDDAIEQILIALLAEGHALIEG
                     VPGTAKTLTVKTLSRIINASFSRIQFTPDLMPSDITGTNVFNIATSNFNLRRGPIFTD
                     ILLADEINRTPPKTQAALLEAMEERQVTIDGEAYPLPSIFTVLATENPIEYEGTYPLP
                     EAQLDRFQLKRLLGYPGEEHEREIIARWNDGFNSKRLDEVDIRPLQDPAAIIHCRAEV
                     ARARMEPGVQHYAVDLVRRTRAHPFIHYGASPRASVALLLCSKALGAIRGREFVTPDD
                     VRDVALPVLRHRLTLRAEAELDGVTPDSVITDILKSAEVPR"
BASE COUNT          430 a          566 c          591 g          471 t
ORIGIN      
        1 cgagtaccat ccagtcagtg atgccttcgc ggtttgcgtc gcgagtgcag atcattgtgc
       61 gcggtgacca ggatatcgtc gaggatgatt tttcgaaaga ctccgagacg gagttatcgc
      121 acgatgattt ggaagcagct cattcgccgg ccccagtcgt gcacattggc aatcgtgaag
      181 gagcgctcct ggtcgattac gcttatgggg cgggccgcat cgtcgtgctc agcgatcctt
      241 acatcgtcac gaacagtggt atcaagttga acgataacct gcaactcgcc atcaatgctc
      301 tcggcggtgg cagaggattg atcgccttcg acgaatatca tcagggcagg ggcgttacgc
      361 aaaatgcttt tgccagttac tttgccggta cgccggtgct ggccctcgcg gcgcagattg
      421 tgttggtgat cctgctcatt ctttggacca atgcgcggcg gttcggacgc ccgttgccat
      481 tgccgcacgt ggatcggcgc tcgagtctgg agtttgttgc gtcgatggct gaattgcagg
      541 agcgttcacg ggcttttgat ctcgccatcg agaatattta cacgcgcacg cgccgcgttc
      601 tcgctcgata tgccggcatg gattacaaca gctcgcgttc tgagatcgcg acgcgcgttg
      661 cagcgcgatc gaatatcgat ggccagaaat tggaaacgct gatgcgtcag tgtgaagaag
      721 cgatcaatgg ggcgccgatt agctggaggc agtcgctcga tctggtgcgg cgcctccgcg
      781 aggttgagag agatcttgga ctgcgcatgc gttcgcgcga cgttcgccag gcagcagaaa
      841 acatttgacc taacgctttg atatttgtac tttgagccca atgcctgaga ccgtcgcctc
      901 tactgtcgaa cacattcgca ccgaattaag aaaagtcatc gtcggacaag acgacgcgat
      961 cgaacagatc ctgatcgctt tgctggcgga agggcatgcg ttgatcgaag gcgtgcccgg
     1021 cacggcgaaa acactgacag ttaaaacgct gtcgcgcatc ataaatgcca gctttagtcg
     1081 catccagttt acgccggacc tcatgccatc cgacatcacc ggcaccaacg tcttcaacat
     1141 cgcgacttcg aatttcaacc tgcgtcgtgg accaatattc acggacatcc tgctggcgga
     1201 tgagatcaat cgcacgccgc caaaaacgca ggcggcactg ttggaagcga tggaagaacg
     1261 ccaggtaacg atcgacggcg aagcgtatcc gctgccttca atcttcaccg tgctggccac
     1321 tgagaacccg atcgaatacg aaggcactta cccactgccg gaagcacaac tcgatcgttt
     1381 ccagctcaag agattgttgg gttacccggg tgaagagcac gagcgcgaaa tcatcgcgcg
     1441 ctggaatgac gggttcaatt cgaagcggtt ggatgaagtt gatatcagac cgctccagga
     1501 tccggccgcg attattcatt gtcgcgcgga agtcgcgcgg gcgcgtatgg agccgggcgt
     1561 tcagcactac gcagtcgatc tcgtgcgccg cacacgtgcg catcctttca ttcattacgg
     1621 cgcgagtccc agggcttcgg tcgcgttgct gttgtgttcg aaggcgctgg gggcaattcg
     1681 cggacgtgaa tttgtgacac ccgacgacgt gcgcgacgtc gcattgcccg tgcttcgtca
     1741 tcgtttgacc ttgcgggccg aggccgagct cgatggtgtc acaccggatt ccgtgattac
     1801 cgacatcctg aaatccgcgg aagtaccaag atagtagccg cagattttcg cagatgaacg
     1861 cagatccaat ccgcgtttat ctgcgaaaat ctgcggctaa atcaaaatgc gttttgtctt
     1921 cacaaagctc ttctaccttc tcgtcgcact cggtctcatc cccttgtggt tttcgtggga
     1981 tcgtccgtgg ctgcgctggg tagcgcttgc ctacaacatc ctgctgatcg gcgctgcttt
     2041 tgccgagtcg cgattctg
//
LOCUS       DASTEY010000589         4090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1331756, whole genome shotgun sequence.
ACCESSION   DASTEY010000589 DASTEY010000000
VERSION     DASTEY010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331756"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2596
                     /locus_tag="VFO72_10325"
     CDS             <1..2596
                     /locus_tag="VFO72_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HET9479732.1"
                     /translation="DSYRRALTALSIGIAYYHLNEFRKALDYFSQTLALSVALKDPRL
                     EAGTHTYAGGMLDLLGDVSKALDHFQRAHKLARAGSWRLAEGNALSNIGKIYNDLADW
                     QKALEFYGQALPVYKSLGNGRQAEAITLNNIGIAYSRSGEQEKALDYLQQSLPLLRAG
                     GDKNSESQTLINIGRVYRLLGDYLKARTYYSQAQAIQKETGNRAQEAETLDEIGVAYS
                     EEGQPEKALEYLPQAVQIQRSLGNARRQGVSLSNLGRVYNLLRQPDKALEHFTQALTI
                     LQGIGDLSSAARVLEGIARAEVSRGNLDVARKHIEESLALSETVRTRASNLQLRSSYR
                     ASVENAYEFYIDVLMQQHAKDPSRGRDEEALRASERGRARNLLEQLSESQFDIRQGVD
                     PALIEKERDLTQVLNAKAQREMQVNARKGSADELAMLRREISALEDEYQQVRAAIRRS
                     SPQYSALTQPQPLGLREIQQQLDPDTLLLEYSLGAERSYLWTVGSDSFKTYILPKQEE
                     IRKVAQHMNESLTARSVVKSLEAPSQRNARIAQADRDFQKAAAELSRMILEPAAAEFG
                     NKRLVVIADDALQYVPFAALSVSSVAYQPLILRHEVVSLPSVSAFAVQRQNLANRAAA
                     PKTLAVIADPVFSTNDPRLKEDTGASSASDSTRSLEHLPATAGMAQLSIPRLPFTRSE
                     ADQILAVARAGSNLKALDFRANRAMATSGELGKYQYVHFATHGYLDTTRAGLSAIVLS
                     LFDEQGRPQNGFLRTHDIYNLKLPAELVVLSACETGLGKDVRGEGLEGLTRGFMYAGA
                     RRVIVSLWNVNDKATASLMQRLYAGMLRSNKTPAAALRAAQIEMLRARQWQSPYYWAA
                     FVMQGEWR"
     gene            2593..3468
                     /locus_tag="VFO72_10330"
     CDS             2593..3468
                     /locus_tag="VFO72_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479733.1"
                     /translation="MKSKAFFLLLILMVASSSFLSTAQTPDEMTRKLWDTAFITPAAS
                     KQTAGRRRRSRAYRVATPNVPTDNVAPDTVVGVTIWRLRPANRSDSGERLIVHGDTAT
                     NEWLPERISANTKLVQGDKLRISVEAVRDGFLYVINREQYADGSLGQPYLIFPTTRTA
                     GGDNEVAIGRLVEIPDQEDAPPFFTMKKSRPDHVAEVLSVLVTPKPLEGVQITDKPLK
                     LTETQVADWEKTWGSSVGRLDMTTVGQAWTKDEKESRTRALTTSAPAPQLVFYRPGAK
                     ATEPMFVKFKLSYRK"
     gene            complement(3483..>4090)
                     /locus_tag="VFO72_10335"
     CDS             complement(3483..>4090)
                     /locus_tag="VFO72_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET9479734.1"
                     /translation="QKMMSMRGNNIHFARATVHHELIPGHHLQGFMSARNKPYRGIFG
                     TPFWTEGNALYWELLLWDLNFAKTPENRIGMLFWRMHRCARIIFSLNFHLEKMTPQEC
                     IDLLVNRVGHERDNATAEVRRSFDGSYGPLYQIAYLIGGLQQYAMHKELVGSGKMTNR
                     AFNDALLKENRIPIEMLRAAITNQKLTRDFVTNWRFYGPRI"
BASE COUNT          915 a         1168 c         1185 g          822 t
ORIGIN      
        1 tgatagttac cgccgcgctc tcacagcgtt gtccatcgga attgcctact accatttaaa
       61 cgagtttcgt aaagcactcg attatttcag tcagacactc gcgctgtcag tcgcgctcaa
      121 agatccgcgt ctggaagccg gaacgcacac ctacgccggc ggcatgctcg atctgctcgg
      181 tgacgtcagt aaagccctgg accacttcca acgcgcccac aagttagctc gagcagggag
      241 ttggcggctt gcagagggca acgcgctcag taacatcggc aagatctata acgatctcgc
      301 cgactggcag aaagcactcg agttctacgg gcaggcacta ccagtttaca aatcactcgg
      361 caatggcaga caggccgaag cgataactct gaataacatc ggtatcgcct acagccgctc
      421 cggcgagcag gaaaaagcgc tcgattatct acagcagtct ctgccgttgc ttcgtgccgg
      481 cggagataag aactccgaat cccaaactct tatcaacatc ggtcgcgttt atcgcctttt
      541 gggtgactat ctgaaggcgc gcacttacta cagtcaggcc caggccattc agaaagagac
      601 tggcaatcgt gctcaggaag ccgagacgct cgacgagatc ggagtcgcat actccgagga
      661 aggtcaaccc gaaaaggcgc tggagtattt gccacaggcc gtgcaaatcc aacgtagtct
      721 gggaaacgcg cgccggcagg gcgtgagcct gagtaatctc ggccgcgtct acaacctgct
      781 gcggcaacct gacaaagctc tcgaacattt cacgcaggca ttgacaatac tgcaaggcat
      841 tggagatttg agcagcgccg cgagagtcct ggaaggcatc gctcgtgcgg aagtgagtcg
      901 cggcaatctg gacgtagcgc gcaaacacat tgaggagtcg ctcgccctaa gcgagaccgt
      961 acggacgcgc gccagcaatt tgcagttacg ttcgtcgtac cgcgcctcgg tcgagaacgc
     1021 ttacgagttt tacatcgacg tgctgatgca gcagcacgcg aaagatcctt cgcgcggacg
     1081 cgatgaggaa gcgctccggg ccagtgaacg aggacgcgcg cgcaacctgc tggagcagtt
     1141 gagtgaatcg caattcgaca ttcgccaggg tgtcgatccg gcgttgatcg aaaaagaacg
     1201 cgatctgacc caagtgctga acgccaaggc gcaacgcgag atgcaagtca acgcgcgcaa
     1261 aggtagcgct gacgaactgg cgatgctgcg tcgagaaatc agtgctctcg aagatgagta
     1321 ccagcaagtg cgggcggcga ttcgccggag cagtccgcag tactcggctt tgactcagcc
     1381 tcagccgctc ggcttacgcg agattcagca acaactcgat cccgacacgc tactgctgga
     1441 gtattcgctt ggcgcggagc ggagttatct ctggacggtt gggtccgact cgtttaagac
     1501 ttacatactt cccaaacaag aagagattcg gaaagttgcg cagcatatga acgagtcgct
     1561 gacggcgcgc agcgttgtca agtcactcga agcaccgtcg caacgcaacg cgcgaatcgc
     1621 gcaagccgat cgagattttc agaaagcggc cgcggagttg agccggatga tcctggagcc
     1681 cgctgcggcc gagtttggga acaagcgcct ggtcgttatc gccgacgacg ccttgcaata
     1741 cgtgcctttt gccgctttgt ccgtgtcatc cgtggcttat cagccactga ttctgcgtca
     1801 cgaagtagtc agtctgccat ccgtttccgc ttttgcggtg caacggcaga acctcgccaa
     1861 tcgtgcggct gcgccaaaga ctctcgccgt catcgctgat ccggtgttct cgacgaacga
     1921 tccgcgattg aaagaagaca ccggtgcgag ttctgcatcc gactcaacgc gaagtctcga
     1981 gcatcttccc gcgactgccg gcatggccca gctctcgata ccacgattgc cgtttacccg
     2041 ttcggaagcc gatcagattc tcgccgtcgc gcgcgccgga tcaaacctca aggcactcga
     2101 cttccgcgcg aaccgcgcga tggcgacgag cggcgagttg ggcaagtatc aatacgtgca
     2161 tttcgcaact cacggctacc tcgatacgac gcgcgccgga ctttctgcga tcgtgctttc
     2221 gcttttcgat gagcaaggca gaccgcaaaa cggtttcctg cgtacgcacg atatctacaa
     2281 cctcaagttg cccgctgaac tggtcgtgct gagcgcgtgt gaaacaggac tcggaaaaga
     2341 cgtacgaggc gaaggtctgg aaggactgac gcgcggtttc atgtatgccg gcgctcgtcg
     2401 cgtgatcgtg agtttgtgga acgtcaacga caaagcgacg gcctcgttga tgcagcgtct
     2461 ctacgccggc atgttacgga gcaacaagac accggccgcg gccttgcgcg ccgcgcagat
     2521 cgagatgctg cgcgccaggc aatggcagtc gccctactac tgggcggcgt ttgtgatgca
     2581 aggagagtgg agatgaaatc aaaagcgttc tttctattgc taatcctcat ggtagcgtcc
     2641 tcttcatttc tgtccacagc tcaaacgccg gacgagatga cgcgcaagct ttgggacacg
     2701 gcttttatca cgccggcagc aagcaaacag acggctggcc ggcggcgccg gagccgagcg
     2761 tatcgcgtag cgacgccaaa tgttcccact gacaacgtgg cccccgacac agtcgttggc
     2821 gttactattt ggcgtctccg cccggctaat cgatcggatt caggcgagcg tttgatcgtt
     2881 cacggcgaca ctgcgacgaa cgagtggctg cccgaacgta tttccgcaaa caccaaactg
     2941 gtccagggag ataaattgcg catcagcgtc gaggcggtgc gtgacggttt tctttacgtc
     3001 attaaccggg agcagtatgc agacgggagt ttgggacagc cttatttgat ctttccgacg
     3061 acgcggacgg cgggcggcga caatgaagtc gcgattggca ggctggttga gattcctgat
     3121 caggaggatg cgccgccgtt tttcacgatg aagaagagcc ggccggatca cgtggcggaa
     3181 gttttaagcg tcctggtgac cccaaagccg ctcgaaggcg tacagatcac tgacaagccg
     3241 ctaaagctga ctgagactca ggttgccgat tgggagaaga cgtggggctc gagcgtcggc
     3301 cgtttggaca tgacaaccgt tggccaggca tggacgaaag acgaaaaaga atcgcgcacg
     3361 cgcgcgttga ccaccagtgc cccggcccca caactcgttt tctatcgccc gggcgccaaa
     3421 gcaaccgagc cgatgttcgt gaaattcaag ctctcgtacc ggaagtaatt ccgtgttgtc
     3481 cgtcaaatcc gtggcccgta aaaccgccag ttggtcacaa agtctcgcgt gagtttttga
     3541 ttggtgatcg cggcgcgcag catctcgatc gggatgcggt tctccttcaa caacgcgtcg
     3601 ttgaatgcgc ggttcgtcat cttgcctgaa ccaacgagct ccttgtgcat cgcgtattgc
     3661 tggagcccgc cgatcaggta cgcgatctga taaagcggcc cgtaactacc gtcgaacgaa
     3721 cgccgtactt cagcagtcgc attgtcgcgt tcgtggccga ctctgttcac cagcagatcg
     3781 atacactcct gcggggtcat cttttccagg tggaagttga gtgaaaagat gatgcgcgcg
     3841 caacggtgca tgcgccagaa gagcatgccg atgcggttct ccggcgtctt cgcaaagttc
     3901 aggtcccaca gcaacagctc ccagtaaagc gcgttgcctt cggtccagaa cggcgtacca
     3961 aatatgccgc ggtaaggctt attgcgcgcg gacatgaagc cctgcaggtg atggcccgga
     4021 atcagctcgt gatgcactgt cgcgcgcgcg aaatgaatgt tgttgccgcg catgctcatc
     4081 atcttttgct
//
LOCUS       DASTEY010000590         5451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_310041, whole genome shotgun sequence.
ACCESSION   DASTEY010000590 DASTEY010000000
VERSION     DASTEY010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310041"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VFO72_10340"
     CDS             <1..1072
                     /locus_tag="VFO72_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET9479735.1"
                     /translation="ETFQQNAKGDLELRQRAIDELIKPLKESLEKVDGRIGELEKTRA
                     GAYSELREQVKTLAQSHLQLQAETGNLVKALRVPHVRGRWGEIQLRRVVELAGMLQYC
                     DFVEQETVNGEDGRIRPDLIVRLPGNRTIVVDSKVPFDAFYESISTTDEEVRASKLKD
                     HARLVRTHIMSLSRKSYWETVQPTPEFVLLFLPGETFYSAALENDPKLIEDGVSQNVI
                     IATPTTLIALLKAISYGWRQEQMAENAQEVSNLAKTLYDRLRVFTNHFDDIGRNLDRA
                     LDAYNKGVRSLETRVLVTARRFKERGAIAGEEIETLEPIDKAARPLTLDEGGLFPESP
                     TALAELDDDFPLLKAHTAGADG"
     gene            1191..4814
                     /locus_tag="VFO72_10345"
     CDS             1191..4814
                     /locus_tag="VFO72_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET9479736.1"
                     /translation="MKEIWLGPLLNNNRALLIERCAKLVTNNQSDRFIYLTASYPLLE
                     TVTQQILDGQRNRGVWGELPVHLFRGFVRRLLYSAVDDNGNGLPPRIPIDHDELPLKR
                     SLISQVLLNLLATNKLKAFAPLAGRDGVVNSVATLLGEIERAAKTPAELIEILETRSR
                     DLEKGGNERRGITQLDFDREVEVIYSTYTELLNQNQFTEEDLDQLRALWVLRGLIDGR
                     GVRVPWLANVELFIIDGFFDFTPVQGEILRQLIPRVPDVVVNLNHDDSNPEIFIPFQE
                     TIGQLQSMATFDTVYTHDYTSPAGLLAQLRQNLFNPLTPSDEVSEEPEFAAKEIRYLE
                     CGDRDTEIRTIAREVKRLVLTENYRLADIALVVRERSRYANTIARVLKEESLPCNLDL
                     RIDAGDVPSNRAALKLLTLLEGLTTDQDEPATRISEIADLIKSEYFRLSDAELQILSK
                     RFEENHSDLLRSDSRTRIGFWDADALENAFAYVGSDLTVSAWLTRAHKLFTDLPGATA
                     TKEVLNIDPGAQDRDTDIADNVENAEIVKLDEKGVEKKRRPSRDIHPSALAWTALVVR
                     RFAELLYAVPREGKLTDLRSELMRLLDRLGFRDQVAVPARRATDEEQLPQTMLNFNSL
                     ETMRRAFVAASKSIEIGGNGVTTARLATFLQEVRRSLGSQSQVFGARDSDGLRVLEAT
                     DVRGLRFRAVFIAGLIEGGFPLAASRDWIYPHEERDRLREYGLTLEDISPNTLLKEEH
                     YFYQSSCRATERLYLTRPLLLEDDTETVPSYYVDELRRAIAPYRVETEIIRRDYDGKN
                     LADASTPNEMKIGLVRQQERHLHRGQREGLLPQPRIKSLLTLARNDGFLSDAALRRIG
                     IERERCGPHYGPYDGIITNPDLLRLLKQKFGSDFVHSASGLSVYGNCPYRFFAQRVLR
                     LEPRGEAALDLQAIDAGKLLHDILRRFFERHRREALHKLDRKKLQTELATIADNVFDE
                     HERVVPPLNKQIWKIDREIRKILLDQVLLYELEVQEASEQEVLPAYFEVAFGGMKSAA
                     KDPDSKDEPLELSRETFVGEETIKISGQIDRVDVARDNTVVAYDYKLSTGSNRSDIIS
                     GRSLQLPIYLEALEKLILPDHPIAGGGYYVIRGAQDRRNKGLYRASQLDYLRLRAKNS
                     VFSDDDWQKIRAEVVTKIWEFLDRMRAGDFRVDPSERLKTCRFCDYRAVSRYDRDRIE
                     RKK"
     gene            4811..>5451
                     /locus_tag="VFO72_10350"
     CDS             4811..>5451
                     /locus_tag="VFO72_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9479737.1"
                     /translation="MKEQRVLLPEQAAAAYEIKKHISVTAGPGSGKTMVLVERYLHIL
                     REHSLSIDQIVAITFTNRAANEMRERLRTDLHHMLRIATDDERRRWLNYKRTLDGAVI
                     TTIHGFCARLLREFPIEARVDPQFLLLDEHRAAMMLELTVEEVLSDFISGGHLEISRL
                     TLGVGRSRLAAALAQIYRETRGQGLSLEELALATGRVHATEEDHARALEDLERT"
BASE COUNT         1235 a         1606 c         1520 g         1090 t
ORIGIN      
        1 cgaaacgttt cagcaaaacg cgaaaggcga cctcgaactg cgccaaaggg ctatcgacga
       61 gctgatcaag ccgctgaagg agtcactcga aaaagtcgac ggccgtatcg gagaactgga
      121 aaaaacgcgc gccggcgcgt attcggagtt gcgcgaacag gtaaagacgc tggcccagtc
      181 gcatttacaa cttcaggcag aaacgggaaa cctcgttaag gcgttgcgcg tgccgcatgt
      241 tcgcgggcgc tggggtgaga ttcagttgcg acgcgtcgtc gagctggccg gcatgctgca
      301 gtattgcgat tttgtcgaac aggaaaccgt caacggcgag gacggtcgta ttcgccccga
      361 tctgatcgtg cgcctgccgg gcaaccggac gatcgtcgtc gactcgaaag ttccgttcga
      421 tgctttttac gagtcgattt caacgaccga cgaagaagtt cgcgccagca agttgaaaga
      481 tcacgcgcgg ctggtcagga cgcatatcat gagcctgagc cggaagtcat attgggaaac
      541 ggtgcagccc acgccggagt ttgtgttgct gtttcttccc ggtgagacgt tttacagcgc
      601 cgcactcgaa aacgatccga agctaatcga agacggcgtc agtcaaaacg taatcatcgc
      661 aacgccgacg actctgatcg cgttgctgaa agcaatctcg tacggctggc ggcaggaaca
      721 gatggctgag aacgcgcagg aagtaagcaa tctcgccaag acactctacg atcgtctgcg
      781 cgtgtttacg aatcacttcg acgatatcgg ccgaaatctc gatcgcgcgc tcgacgctta
      841 taacaaaggc gtgcgctcgc tcgagacgcg cgtgctcgtg actgcgcggc gttttaagga
      901 gcgcggcgcg attgccggcg aagagatcga gacccttgaa ccgatcgata aagccgcccg
      961 accgttgact ctcgatgaag gcggactctt cccggaatcg ccaaccgccc tcgctgaatt
     1021 agacgacgat ttccctctcc tcaaagccca cacggcgggc gccgacgggt agacagcatt
     1081 tcagaattga caagagcgac atgcctccag gaacaatcct gcaagttctg aaatcctgtc
     1141 taagtgccgg tgacaggcga aaataatctg tgtaagctgc ggttactgct gtgaaggaaa
     1201 tttggttagg tcctctacta aataacaacc gagcgctgct catcgagcgc tgcgccaaac
     1261 tcgtcacgaa taatcaaagc gatcgattta tttatctaac cgcttcgtat ccgctgctcg
     1321 aaactgtcac gcagcagatt ctcgacggcc agcgcaatcg cggcgtttgg ggcgaactgc
     1381 cggtccacct gtttcgcggt ttcgtgcggc gcttgttgta ttcagccgtc gacgacaacg
     1441 gcaacggtct cccgccgcgc attccgatcg atcacgacga gttgccgcta aaacgcagct
     1501 tgatctccca agtgctgctc aacctgctcg cgaccaacaa actgaaagca ttcgcgccac
     1561 ttgccggtcg cgacggtgtc gtcaacagcg tggccacact actcggcgag atcgagcgcg
     1621 ccgccaaaac tcccgcggaa ctgatcgaga tcctcgaaac acgttcgcgc gatctggaaa
     1681 agggaggcaa cgaacgccgc ggcatcacac aactcgattt cgacagggaa gtcgaagtca
     1741 tctactccac ttacactgag ctactcaatc agaaccagtt cacggaagaa gatctggatc
     1801 aactgcgtgc gctgtgggtg ttgcgcggct tgatcgacgg ccgcggcgtg cgcgtgccgt
     1861 ggcttgcaaa cgtggagctc ttcatcatcg atggcttttt cgacttcacg cccgtgcaag
     1921 gcgagattct gcgccagttg atcccgcgcg tgcctgatgt cgtcgtgaat cttaatcacg
     1981 acgattccaa ccccgagatc ttcattccgt ttcaagagac gatcggccaa ctgcaaagca
     2041 tggcgacgtt cgacaccgtg tacacacacg actacacatc gcccgccggt cttctcgcgc
     2101 agctccgcca gaatctcttc aatccgttga cgccatcaga cgaagtctca gaagagcccg
     2161 agtttgccgc aaaggagatt cgctatctgg aatgcggcga ccgcgatacg gaaatccgca
     2221 ccatcgcgcg tgaagtgaag cgtttggtgc tgaccgaaaa ctaccgtctc gccgacatcg
     2281 cgctggtcgt gcgcgagcgt tcgcgctacg ccaatacgat tgcgcgcgtg ctcaaggaag
     2341 agtcgctgcc gtgcaatctc gacctgcgga tcgacgctgg tgacgttccg tcgaatcgtg
     2401 cggcgttgaa actgctcacg ctgcttgaag gtctgaccac ggatcaagat gagccagcaa
     2461 cgcgtatttc cgagatagcg gacttgatca aatccgaata cttccggctt agcgatgctg
     2521 agttgcaaat tttgtcgaag cgttttgaag agaatcacag tgatctcttg agatcagatt
     2581 cgcgaacgcg catcggcttc tgggacgccg acgcgcttga aaacgcattc gcttacgtcg
     2641 gcagcgatct taccgtcagc gcatggctca cacgcgcgca caaactgttc accgatctgc
     2701 ccggcgcaac cgcaacgaaa gaagttctga acatcgatcc cggcgcgcag gaccgcgata
     2761 ccgatatcgc cgacaacgtc gaaaacgccg agatagttaa actcgatgaa aaaggcgtcg
     2821 agaaaaaacg gcgcccgtcg cgcgatattc atccatccgc gcttgcctgg acagcgctcg
     2881 tcgtgcgccg ctttgccgaa ctgctttacg ccgtgccgcg cgaaggcaaa ctgacggatc
     2941 tgcgctcgga gttgatgcgg ctgctcgatc gtcttggttt tcgcgatcaa gttgccgtac
     3001 ccgcccgccg agcgacggac gaggaacaac tcccgcagac gatgctcaac ttcaattcgc
     3061 tggaaacaat gcgccgcgct ttcgttgcgg ccagcaagag catcgagatt ggcggcaacg
     3121 gagtaacgac ggcacgtctc gcgacattcc tgcaggaagt gcgacgctcg cttggttcgc
     3181 aatcgcaagt cttcggcgca cgcgatagcg acggtttgcg tgtgctggaa gcaaccgacg
     3241 tgcgtggtct gcgttttcgt gctgtgttca ttgccggatt gattgaaggc gggtttccgc
     3301 tggcagcttc gcgcgactgg atctatccgc atgaagaacg cgatcgcctg cgcgagtatg
     3361 gtctgacact cgaagacatc tcgcccaaca cactccttaa agaagaacac tatttctatc
     3421 agtcgtcgtg ccgcgcgacc gagcgtctct atctcacccg cccgctgtta ctcgaagacg
     3481 acaccgaaac cgtcccgtct tactacgtcg acgaactgcg ccgcgcgatc gcgccgtacc
     3541 gcgtcgaaac tgaaatcatt cgtcgcgatt atgacggcaa aaacctcgcc gatgcttcaa
     3601 cgccaaacga aatgaagatc ggcctggtgc gccagcagga gcgccacctg catcggggtc
     3661 aacgagaagg actcttgccg cagccgcgca ttaagagtct gctaacactc gctcgcaacg
     3721 acggttttct cagcgacgca gccttgcggc gaatcgggat cgaacgcgag cgttgcgggc
     3781 cacactacgg tccgtatgac ggcatcatca caaatccgga tctcctgcga ttactgaaac
     3841 aaaaatttgg ttctgatttt gttcacagcg ccagcggttt aagcgtttat ggtaattgtc
     3901 cttaccggtt tttcgcgcaa cgcgtgctgc ggctcgaacc acgcggcgaa gcagcgctcg
     3961 acttacaggc catcgacgcc ggcaaactcc tacacgacat cctgcgacgt ttcttcgagc
     4021 gacaccgccg cgaggcgctg cacaaactcg atcgcaagaa actgcaaacc gaactcgcca
     4081 cgattgccga taatgtgttt gacgaacacg aacgggtggt gccgccgctc aacaaacaga
     4141 tctggaagat cgatcgcgag attcgcaaaa tccttttgga ccaggtgctg ttgtacgaac
     4201 tggaagttca ggaggcgtcg gagcaggaag tgctgccggc ttatttcgag gttgccttcg
     4261 gcggtatgaa atctgccgca aaggatccgg attcaaaaga cgagccactg gaactctcgc
     4321 gcgagacgtt tgtcggcgag gagacgatca agatcagcgg gcagatcgat cgcgtggacg
     4381 tcgctcgcga caacaccgtc gtcgcctacg actacaaatt gtcaaccggc tccaatcgca
     4441 gcgacatcat ttccggccgc agtctccaat tgccaatcta tctggaagcg ctcgaaaaac
     4501 tgatcctgcc cgatcatccg attgccggtg gcggctacta cgtgattcgc ggcgcgcagg
     4561 atcgccgcaa caagggtctc tatcgcgcga gccagttgga ttacctgcga ctgcgcgcga
     4621 agaattccgt tttctctgac gacgactggc aaaagatccg cgctgaagtc gtgacgaaga
     4681 tctgggagtt tctcgatcgg atgcgcgccg gggattttcg tgtcgatccg tccgagcgtc
     4741 tgaaaacttg ccgcttttgc gactaccgcg ctgtgagccg ctacgaccgc gatcgaatcg
     4801 aaagaaagaa atgaaagaac aacgcgtgct gttgcccgaa caggccgcag ccgcatacga
     4861 gatcaagaag cacatttcag tcacggccgg acctggctcg ggcaagacca tggtcctggt
     4921 cgagcgctat ctccacatcc tgcgcgagca cagtctttca atcgatcaga tcgtcgccat
     4981 cactttcacc aatcgcgccg ccaacgagat gcgcgaacgc ctccgcacgg acctccatca
     5041 catgttgcgc atcgcgaccg acgacgagcg aaggcgctgg ctgaattaca agcgcactct
     5101 cgacggcgct gtgatcacta cgattcatgg tttctgcgcg cgattgctgc gcgagtttcc
     5161 gatcgaggct cgggtcgatc cgcagttcct gctgctggac gaacaccgcg cggcgatgat
     5221 gctggagttg accgttgaag aggttctttc tgatttcatc agcggcggtc atctcgagat
     5281 ctcgcgtctg acgctcggtg tcgggcgtag ccgcctcgcc gcagcgctag cgcagatcta
     5341 ccgcgaaacg cggggccagg ggttgtcact cgaagaactc gcgcttgcca ccggtcgcgt
     5401 ccacgccacc gaagaagatc acgcgcgcgc cctcgaagat ctcgaacgca c
//
LOCUS       DASTEY010000591         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1282518, whole genome shotgun sequence.
ACCESSION   DASTEY010000591 DASTEY010000000
VERSION     DASTEY010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282518"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..311)
                     /locus_tag="VFO72_10355"
     tRNA            complement(237..311)
                     /locus_tag="VFO72_10355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Gly,seq:ccc)
     gene            complement(432..959)
                     /locus_tag="VFO72_10360"
     CDS             complement(432..959)
                     /locus_tag="VFO72_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET9479738.1"
                     /translation="MSDDLQLRAARIKLFLMDCDGVLTDARIWILESGEDQKAFNTRD
                     GLGLELLHRAGLQSGIISGRVSGALQRRADWLGMKYVRQGCQDKEQAFAEVLAEAGLT
                     NAEVAFAGDDLNDVPLIVRAGLGVAVADATAEAREHAHYVTQARGGHGAVREAIELIL
                     KSQGRWDDLVRHYLR"
     gene            complement(986..>3031)
                     /locus_tag="VFO72_10365"
     CDS             complement(986..>3031)
                     /locus_tag="VFO72_10365"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479739.1"
                     /translation="GAAGTFRPGAEPRADLQRVEISLPASIAPGGSTSVTVTYALRVE
                     ANSGLSAISPIGTQFLPLSFWYPMPNTPYTLRGADTAPFRLTVNLPNVVSSGVDKTAA
                     GGSVSFEQTLHSQPFLVQGDWEKIEGAADAKSIVVLMEKGMGAEERKRAEALIALAGA
                     ARSFYETTFGPAPDVPIRLVSVRRGAGFSDGGTLLVDSDALRLPKIDAATALSVAETV
                     ARLWIGGQAPVRGEGGGVLRDALVRFLGTQFLEKHFGREAAQSELYRQRIAYAAVAQR
                     DGPLTLASQLDSTYFGSVPNRGAMFWRLVERKLGRDAFVSVLKAAVQSGKTDQSGLTL
                     TGLREALAARGGESLKSLMDQQLDQVVDTDLMVGLPQQRGADWVSMLRNIGSIDVTVT
                     VAATTERGETVLAEATVPARDFGEAVFKTPAKIVRVEVDPEKFYPQLDYGNDVVPRAK
                     DLPDALNEATLQLGAQDFVKAEATARAMLNTAPRFMEARVLLARALLGQNKIEEAEKL
                     FRSALDEGLPFPATLAWANIGLGEIAMKRGQHAEAAKRFSDAVVASRDYPSSLAARLA
                     RIRAEAAGNIAPPVDPAVQAFVTQLSQAVVSNKKAELESRIVSGELVRFINASVGTQA
                     WETKVVRTEQLNANLIAADVQIRLNKLGTVGSGTAVLMLARTPAGLKLSGIELFEVR"
BASE COUNT          689 a         1000 c          759 g          583 t
ORIGIN      
        1 gaaaaccgtt gaaacggttt cctccgtctt cactgctcct gcaccgggct taaagcccgg
       61 tgagaatgag agaggacttt tgaggcaaaa catcgtggag tatagagtga cccaacaact
      121 accgtcaata gtcgtacaaa gctggcctaa gtaacacccg tcgcttgaaa tttggctgat
      181 cagggggagg gttgggattt actgagggaa cgcgcgatgc gttttcttca agaatctgga
      241 gcgggcgacg ggattcgaac ccgcaaccct cagcttggga agctgatgcg ctaccgttgc
      301 gccacacccg ctcgggtttt gcggaaacta aataccacag atctgctgaa atgaacacgc
      361 gtcagcgaat gaatatggtc ggcgaatgaa catgcgccat ccatgaggac gtgtcagtga
      421 cccgaatact gtcagcggag gtagtgccgc acgagatcat cccagcggcc ctgcgacttc
      481 agaatcaact caatcgcctc gcgcacagcg ccgtgtccgc cacgcgcctg cgtgacgtag
      541 tgcgcgtgtt cgcgagcctc ggctgtcgca tccgcaaccg cgacgcccaa acccgctcgc
      601 actatcaaag gaacgtcatt gagatcgtca ccggcaaacg caacttccgc gtttgtcaga
      661 cccgcttcag ccaagacctc agcaaacgcc tgctccttgt cctgacatcc ctgccgcacg
      721 tacttcatcc cgagccaatc agcccgacgt tgcagagcgc ctgacacacg gccggaaata
      781 atccccgact ggagccccgc gcgatgcagc aactcaagcc cgagtccatc gcgcgtgttg
      841 aaagctttct ggtcttcccc gctctcgaga atccagatcc gcgcgtcagt cagcacgcca
      901 tcgcagtcca tcagaaacaa cttgatgcgc gcggcacgca actgtaagtc atcgctcata
      961 gccacagttc acacaaacta acgaattacc gcacttcgaa gagttcgatt cccgagagct
     1021 tcaaacccgc aggtgttcgc gccagcatca ataccgctgt gcccgaacca accgtgccta
     1081 gtttgttcaa gcgaatctgc acgtcggctg cgatcaggtt tgcgttgagt tgttcggtgc
     1141 gcacgacctt cgtctcccac gcttgagtgc caacgctcgc attgatgaac ctcaccaact
     1201 cgcccgaaac aattcgcgac tccaactccg cctttttatt actcaccacc gcctgcgata
     1261 gctgcgtcac aaacgcctgc accgccgggt cgaccggcgg agcaatgttg cccgccgcct
     1321 cagcgcgaat tcgtgccaga cgcgctgcca acgacgatgg ataatcacga ctcgccacca
     1381 ccgcgtcact aaaacgcttc gctgcttcag cgtgctggcc gcgcttcatc gcaatctcac
     1441 cgagcccgat gttggcccag gccagcgttg ccggaaacgg caatccctcg tcgagtgccg
     1501 aacgaaacag cttctcggct tcttcaattt tgttctggcc cagcaaagca cgcgccagga
     1561 ggacccgtgc ttccataaaa cgcggcgcag tattcaacat cgcccgagcg gtagcttccg
     1621 ctttgacgaa gtcctgcgcg cccaactgca acgtcgcttc atttaaagcg tcaggcaagt
     1681 cctttgcgcg cggcacgacg tcgttaccgt agtcgagctg cgggtagaac ttttccggat
     1741 cgacttcaac gcgaacgatc ttcgcagggg tcttgaacac ggcctcccca aaatcgcgcg
     1801 ccggtacagt cgcttccgcc aacaccgttt caccacgttc cgtcgtagca gccaccgtca
     1861 ccgtcacatc aatcgagccg atattacgca gcattgaaac ccaatcagcg ccacgctgtt
     1921 gtggcaaacc aaccatcagg tccgtatcaa ctacctgatc gagttgctgg tccatcaaac
     1981 tcttgagact ctcaccgccg cgtgctgcca gcgcctcgcg taagccagtc aacgtcaatc
     2041 cactctgatc tgtcttgccg ctttgcacag ccgcctttaa cacgctcaca aacgcgtcgc
     2101 ggcccagctt gcgctccacc aggcgccaaa acattgcgcc acgattcggc accgatccga
     2161 aatacgtact gtccaactga ctcgcaagcg tcagtggacc atcgcgctgc gccactgccg
     2221 catacgcaat ccgctggcga tacaactcag actgcgcggc ttcgcggcca aagtgtttct
     2281 ccaaaaactg cgtgcccaga aaacgcacga gcgcatcgcg caagactcct ccaccttcgc
     2341 cacgcacggg cgcctgtccc ccgatccata aacgcgcgac cgtctcagct accgagagcg
     2401 ctgttgcggc atcgatcttc ggcagacgca acgcgtcaga atcgaccaac aaggtgccgc
     2461 catcgctgaa tccagcacca cgtctgacag acaccaacct gatcggtacg tcgggcgctg
     2521 gaccaaacgt cgtctcatag aaagatcgtg ctgcgccggc caaagcgatc aaagcctcgg
     2581 cccgcttacg ctcctcggcg cccatgccct tctccatcaa cacgacgatg ctcttagcgt
     2641 cggcagcgcc ttcaatcttc tcccaatcgc cctgaacaag gaacggttga ctatgaagtg
     2701 tctgttcaaa agagacggat ccaccagcgg cagtcttgtc tacaccggac gagactacgt
     2761 ttgggagatt cacagtcaaa cgaaacggcg ccgtgtctgc accacgcaac gtgtaaggcg
     2821 tgtttggcat cggataccag aacgacagcg gcaggaactg tgtaccaatc ggactgatgg
     2881 ccgagagtcc actgttggcc tcaacccgca gcgcgtaagt caccgtaact gaagtcgaac
     2941 cgcctggcgc aattgaagcg ggcaaagaga tctcgactcg ttgaagatct gctctgggtt
     3001 cggcgcccgg ccgaaacgtt cctgcggcac c
//
LOCUS       DASTEY010000592         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1447592, whole genome shotgun sequence.
ACCESSION   DASTEY010000592 DASTEY010000000
VERSION     DASTEY010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447592"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFO72_10370"
     CDS             <1..305
                     /locus_tag="VFO72_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479740.1"
                     /translation="QYSWWGPMAWRVGSLGMWLYKLRGLNGPNFTWPLLMFSGAISEK
                     TLQRMGKIYAGKPLKSKNRRELLASLKPKDWQYLRADNGDMPIQYEPRTPQMMATA"
     gene            372..1013
                     /gene="can"
                     /locus_tag="VFO72_10375"
     CDS             372..1013
                     /gene="can"
                     /locus_tag="VFO72_10375"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HET9479741.1"
                     /translation="MKDLKRLLEQNKAWAEGLKASDPEFFQALAKQQSPTFLWIGCSD
                     SRVPATQLVGMLPGEMFVHRNVANVVVHTDFNCLAVMQFAVDVLKVDHIIVCGHHGCG
                     GVKAAMDNSQLGLIDNWLRHVQDVMHAHTDALAKIDDDSERLDRLCELNVIEQVINVS
                     RTTIVQNAWQRRQELVVHGWIYGLQDGLLRDLGITIDNAGGLAAAHREAISRM"
     gene            1020..1256
                     /locus_tag="VFO72_10380"
     CDS             1020..1256
                     /locus_tag="VFO72_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479742.1"
                     /translation="MFQRLFNITLLVVVAIVSIVFIAFLLAGLISMVNPPLLSGSSGI
                     SAVAGGVSEKSLRLVYLVLLLAAVIYLWRRHRSR"
     gene            complement(1248..2063)
                     /locus_tag="VFO72_10385"
     CDS             complement(1248..2063)
                     /locus_tag="VFO72_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9479743.1"
                     /translation="MGRTRALMHITSRDNSLLRQARAVRDGKVDDLIFVEGLRLCVEA
                     LRSNLELEAVIASEELIRKEKAAESFTEISKASRRVGTVSEKLLESISYTKTPQGIVV
                     LARRPEASEQRLAASLTANSLLVVLHQINNPVNVGAILRTAEAAGATGIITSKNTSDP
                     FSPKSLRGAMGSAFRLPIWQDAEFKRAVEWCRRYELVTIGTDADSKTKHTDIDWRRPT
                     ALVLGPESTGLTADELQNTDQTVSIPMQGEVESLNVSVAAGILLYEAARQRSS"
     gene            complement(2042..>3000)
                     /locus_tag="VFO72_10390"
     CDS             complement(2042..>3000)
                     /locus_tag="VFO72_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9479744.1"
                     /translation="FDAKNMSSTTATTVDIKIPPLSDAEKLEFGEQNNHHAISPNRNR
                     LLVASYNIRYARGPYLLSGGLRRKLGLMSVRNRPRHVGRLIADAARAFSSGQLLPRVD
                     VLALQEADKRTVRTGGHHVAQELARQLEMNWVHAPAGIPRGIKPMKRQWWLDFEEPIA
                     LHDAGDTGVALLSRLPMSDVMRIDLPWEECPWRPRLAMAATVSQVRIFNAHVDPHAAC
                     GGQLAQLEVIATQADAVAGPVVIMGDFNTLSKEKCLETRKFLESRGYTTPIPTGTATW
                     RGAGLRMHADWIFARGLKFVRWGVARPLHVSDHWPIWAELEL"
BASE COUNT          586 a          846 c          895 g          673 t
ORIGIN      
        1 gccagtacag ttggtggggg ccgatggcct ggcgcgtcgg ctcactcggg atgtggctct
       61 acaagttacg cggacttaat ggtccaaact tcacctggcc attgctgatg ttctccggtg
      121 cgatctccga aaagacccta cagcgcatgg gcaagatcta tgcggggaag cctctcaagt
      181 cgaagaaccg gcgcgagttg ctcgcgtcgt tgaaaccgaa agactggcaa tacctccgtg
      241 cggacaacgg cgacatgccg attcagtacg agccacggac tccacagatg atggcgacag
      301 cttaagcgcg aaatcgttgc tccgagggaa gttgccggtg cgtctcatag gcgttagtct
      361 gtggctgaat tatgaaggat ctcaagcggc ttctcgaaca gaacaaggcg tgggctgagg
      421 gtctcaaggc aagcgatccc gagttctttc aggcactcgc gaaacaacaa tcgccaacgt
      481 ttctctggat cggctgttcc gacagtcgcg tgccggccac gcaattggtg ggcatgcttc
      541 cgggtgagat gtttgtgcac cgcaacgtcg cgaatgtggt tgtccacact gacttcaatt
      601 gtttggcggt gatgcagttc gcggtggacg tgttgaaggt ggaccacatc atcgtctgcg
      661 gtcatcacgg atgcggcggc gtcaaagccg cgatggacaa ttcgcaattg ggtttgattg
      721 ataactggct tcgtcacgtt caggacgtga tgcacgcgca tacagacgcg ctggcgaaga
      781 tcgatgacga ctcagaacgt ctggaccggc tgtgcgaact gaacgtcatc gagcaggtga
      841 tcaacgtaag ccgtacgacg attgtgcaga acgcctggca gcgccgtcag gagttggtgg
      901 tgcacggctg gatttacggt ttgcaggacg gtctcttgcg cgacctcggg attaccatcg
      961 acaatgccgg tggtttggcg gctgcccatc gcgaggcaat atcgcgcatg tgagggctca
     1021 tgtttcagag actcttcaac attacgctcc tggtcgttgt ggcgatcgtt tcgatcgtat
     1081 ttattgcttt tctcctggcc ggtctgattt caatggtgaa tccgccgctg ctgagcggta
     1141 gttccggaat cagtgcagta gcgggtgggg tttcggagaa aagcttgagg ctggtgtatc
     1201 tcgtactgct cttagcggca gtgatctatc tttggcgccg tcacagatca cgatgagcgc
     1261 tggcgagcgg cttcgtataa aagaattcca gcggctactg aaacgttcaa actctccact
     1321 tcaccttgca ttggtatgct gacggtttga tcggtgtttt gtaactcgtc agcggtgagg
     1381 ccggtcgact ctggtccaag caccagtgca gtcgggcgcc tccaatcaat atccgtgtgt
     1441 tttgtctttg aatctgcgtc cgtgccgatc gtgacaagtt cgtacctgcg acaccactcc
     1501 actgcccgtt tgaactcggc gtcttgccag atcggcagcc ggaaagcgga tcccatcgct
     1561 ccgcgtaacg acttcggtga gaatggatcg ctcgtgtttt tgctggtgat tatgccggtt
     1621 gccccggcag cttcggcggt ccgcagaatc gcgccgacgt tcaccggatt gttgatctgg
     1681 tgcaacacca cgagcagcga gttggcggtc aaacttgccg ccagtcgctg ttccgaagct
     1741 tcaggtcgtc gtgccagcac gacgatgccc tgcggcgtct tcgtgtaaga gatcgactcc
     1801 agcagtttct cactgaccgt gcccacgcgg cgtgacgctt tcgagatttc ggtaaaggac
     1861 tcggcagctt tctctttgcg gatcaattcc tccgacgcga tcactgcttc gagttcgaga
     1921 ttcgatctga gcgcctcaac gcacagccgc aatccctcca cgaaaattaa gtcgtcaact
     1981 ttgccgtcgc gcacagcgcg tgcctggcgg agcagggaat tgtcgcgact ggtgatgtgc
     2041 atcaaagctc gagttctgcc caaatgggcc agtgatcgga aacgtgcaaa ggccgcgcga
     2101 cgccccaacg cacgaacttc agcccgcgcg cgaagatcca atcggcgtgc atccgcagtc
     2161 ccgcgccacg ccacgttgcc gtgccggtcg gaatcggcgt cgtgtaaccg cgcgattcga
     2221 gaaacttcct cgtttcgaga cacttctctt tcgatagcgt gttgaagtca cccatgatga
     2281 ccaccggacc agcaactgca tccgcttgcg tcgcgatcac ctcgagttga gccaattgac
     2341 cgccgcaggc cgcatgtgga tcgacgtgcg cattgaaaat acgaacctgt gagacagtcg
     2401 ctgccatcgc gaggcgtggc cgccacggac actcctccca gggcagatcg atccgcatca
     2461 cgtcgctcat gggcaagcga ctcagcagtg ccaccccagt gtcgccggca tcgtgcagcg
     2521 cgatcggttc ctcgaagtcg agccaccact gtcgcttcat tggtttaatg ccgcgcggaa
     2581 ttcccgccgg cgcgtgcacc cagttcatct ccaactgcct agctaattcc tgcgcgacgt
     2641 ggtgaccgcc cgtgcgaaca gttcgcttgt ctgcttcctg caacgccaaa acatcgacgc
     2701 ggggcaggag ctggccgctg gaaaacgccc gcgccgcatc cgcgatgagc cggccgacgt
     2761 gtcgcggacg attgcgcacg ctcatcaggc cgagtttgcg gcgcaggccg ccggagagca
     2821 gataaggtcc gcgcgcgtaa cggatgttgt atgatgcgac gagtaaacgg ttgcggttgg
     2881 gactgatcgc gtggtggttg ttctgttcgc cgaattccag tttctcggcg tcgctaagcg
     2941 gggggatttt gatgtccacg gttgtagcag ttgttgacga catgttcttc gcgtcgaaga
//
LOCUS       DASTEY010000593         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_969326, whole genome shotgun sequence.
ACCESSION   DASTEY010000593 DASTEY010000000
VERSION     DASTEY010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969326"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFO72_10395"
     CDS             <1..967
                     /locus_tag="VFO72_10395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9479745.1"
                     /translation="KSLLGRNSDYDFTDKVVLITGGSRGLGLVMAREFAREGAHLVIC
                     ARDEDELQRARLDLESRGAQVMTHQCDVTSRVDVNEMIAAVEARFGGVDVLINNAGII
                     QVGPLEVMTHADFEKAMNSHFWAPLNTTMAVLPRMRQKKSGRIVNISSIGGKVSVPHL
                     VPYCASKFALTGLSRGLRTELLKDGIAVTTICPGLMRTGSPRNAGFKGKHREEYAWFS
                     ISDALPLLSVSAERAAREIMNACRSGRAELVISAPAKLAVLFDTLLPEITSNLFALAN
                     RFLLPEPGGIGEREAKGKESTSSLSPSWLTTLNERAAVRNNEVLT"
     gene            1130..1555
                     /locus_tag="VFO72_10400"
     CDS             1130..1555
                     /locus_tag="VFO72_10400"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9479746.1"
                     /translation="MQKVLFLCTGNSARSIFAEYLLRRAGGDRFEVYSAGASPTGKVN
                     PFALRVLKDVYDIDASSARSKSWEEYDGIEFDFVITVCDNARESCPIWPGHPAMSHWS
                     LPDPALARGSDEQIYETFKAVAADIERRVESFISDRSGR"
     gene            1802..>2086
                     /locus_tag="VFO72_10405"
     CDS             1802..>2086
                     /locus_tag="VFO72_10405"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET9479747.1"
                     /translation="MHQDWGKLLFMHWRVEPSLIRHLIPDALELDLYGGNAWIGIVPF
                     TMWDIRAFPPLAPPVPGLDSMHELNVRTYVHYQGVPGVWFFSLDVNSLVAA"
BASE COUNT          443 a          552 c          632 g          459 t
ORIGIN      
        1 taaatcactt ttgggccgca attcggatta cgactttacc gacaaagtgg tgctgatcac
       61 cggcggttcg cgcgggctcg gattggtgat ggcgcgcgaa ttcgcgcgtg agggtgctca
      121 cctggtgatc tgcgcgcgcg acgaagacga actgcaacgt gcgcgactgg atctcgagag
      181 tcgcggcgcg caagtgatga ctcaccagtg cgacgtcacc agtcgggtgg acgtaaacga
      241 gatgatcgcg gccgttgagg cgcgtttcgg tggagttgat gtcttaatca acaacgccgg
      301 catcatccag gtgggcccgc tcgaggtcat gactcacgcc gatttcgaga aagcaatgaa
      361 ctcgcatttt tgggccccac tcaataccac gatggccgtc ttgccgcgca tgcgtcagaa
      421 gaagagcggg cgcattgtga acatctcttc gattggcggc aaggtaagtg tgccgcatct
      481 cgttccttac tgcgccagca agtttgcgct gactggattg tcgagaggtt tgcgaacaga
      541 gttgctcaaa gacgggattg cagtcacgac gatttgcccg ggactgatgc ggacgggcag
      601 tccgcgaaac gcgggtttca aaggcaaaca tcgcgaggag tacgcgtggt tcagcatcag
      661 cgatgcgtta ccgctacttt cggtgagtgc ggaacgcgca gcgcgagaga ttatgaatgc
      721 gtgccggagc gggcgtgcgg agttggtgat ctcggcgccg gcgaagctgg cggtgctgtt
      781 tgatacgcta ctgccggaga taacgtccaa cctattcgcg ctggcaaaca ggttcctgct
      841 gcctgagccg ggcggcatcg gcgagcgcga agcgaagggc aaggaaagca cctcaagttt
      901 gtcgccctca tggctcacca ctctcaacga gcgcgccgcc gtgcgcaaca acgaggtgct
      961 gacctaaccg ttttcgctcc aggataccgg tgaatatgcg gcttgacata tgcttgttcc
     1021 ctcggcatac ttcgcggggc atatacgctg gagcgtatgt gtggaaagag gaagtgacaa
     1081 gagtgtgcta agcctgccct cggcgactta gcgcaaccgg agacaacacg tgcagaaagt
     1141 tctgtttctt tgcacgggta attcggcgcg aagtattttt gccgaatacc tattgcgacg
     1201 cgccggcggt gaccgatttg aagtgtacag cgcgggagct tccccgacgg gcaaggtcaa
     1261 tccgtttgcg ctccgtgtac tgaaggatgt ttacgacatc gacgcgagtt cagcgcggag
     1321 caaatcgtgg gaagagtatg acggcatcga attcgatttc gtgatcactg tctgtgataa
     1381 cgcgcgtgag tcgtgtccta tttggcccgg ccatccggcg atgtcgcact ggagtttgcc
     1441 cgatcctgct ttggcgcgag gatcggacga gcagatttac gagactttca aagcggtcgc
     1501 cgcggatatc gagcgtcgcg tcgaatcgtt catctccgat cgttcgggtc gatagcggat
     1561 tgctgatgtc cgtgcatatc tagagcggta gcatgtattt agaacaatca aaagtaagtt
     1621 gttcgtgtac caaaaacagg cgtgggcact caggcaaacc acctagaaca actggtctaa
     1681 catcggcacc taagttgctc aactaaagcc gaacattttt atgaaggcgc ctatcaccac
     1741 tcgccatccg actgacgcgg accgcctcgc cattaggcat cgcccaggcg gccaaccggt
     1801 catgcatcaa gactggggca aactcctgtt catgcattgg cgagtagaac caagcctcat
     1861 ccggcacttg attcctgacg cgctggaact cgatctgtat ggcgggaacg cgtggatcgg
     1921 aattgtgccg tttacgatgt gggacattcg cgcgtttcca ccgcttgcgc ctcctgttcc
     1981 cggtttggac tcaatgcacg aactcaacgt gcgcacctac gtccactatc aaggcgttcc
     2041 aggtgtgtgg ttcttttccc tggatgtgaa cagtctggtg gcggcg
//
LOCUS       DASTEY010000594         5094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_277095, whole genome shotgun sequence.
ACCESSION   DASTEY010000594 DASTEY010000000
VERSION     DASTEY010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277095"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1570)
                     /locus_tag="VFO72_10410"
     CDS             complement(386..1570)
                     /locus_tag="VFO72_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9479748.1"
                     /translation="MATVKDVIVRLGADDHPPVTGLVARYRRRDFFLSRWRITEFDEH
                     GVRLNSDILDLRPFVRRESEVLLARDVLDKQLIDVDGKRVVRVNDVQIIHAADEWRVT
                     GADVSLQGLWRRLAPAGFAGTRKPVEVLDWADVGYLATDAATVQLKSSSDKLARLHPV
                     EIARLAEALSYHHGSEVVESLDDETAAETLEEMPAEHQARILGDMDQERAADILEWMS
                     PDEAADVLGDLPEEKAEELLGLMEDEEQAEVAELLPYEDDTAGGLMTTEFVTLPRQLT
                     VGEALARLREMAETPNMIYYLYVVEEENSWKLSGIIALRSLILADPSMPLENVMRTDF
                     QQAHPHEPAREVAHRIAEYNLLALPVIDESGDILGIITVDDAMEILLPKDWRQRLPRL
                     LG"
     gene            complement(1676..2986)
                     /locus_tag="VFO72_10415"
     CDS             complement(1676..2986)
                     /locus_tag="VFO72_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9479749.1"
                     /translation="MDRSSRHSSKVLLLLAVLCLFVSQLTISSHGASDDPSLVSTTTA
                     EGRLVVFDDVWETIQDRYYDPRFNGVDWHAKRTWFRPAAAKAANTHEFYEVMRQMIAS
                     LRDAHTRVYSPDEKFDWWNPRFITVGVSVREVDGVPVVVQVEPNSAAARTDIRVGDAI
                     VSIDDLPVAEFIKERLENFGADQRARYRSIASLFDGLSGSSVKVAWATREGKLKSAVL
                     QRYWSQRQLGFTTQRKGKLAILKIDAFTQSLALEFSRALAPALEGAEGIVLDLRANGG
                     GDAEAMADVASLFLVDGVNLGKFADRSGASFELQTYSKRLWRVPRLSQIKLPLVVLTS
                     ESTSSAAEIMAAALQARSRARVIGTATCGCVLAIRNRHALPDGGLLDVSEFDYRTAGG
                     IRLQGRGVTPDQLAPLARSDLYTRRDPALEAARNFLERPVKSTL"
     gene            complement(2990..3868)
                     /locus_tag="VFO72_10420"
     CDS             complement(2990..3868)
                     /locus_tag="VFO72_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9479750.1"
                     /translation="MIRAFCAICALLFFLETAHAQSPKPTLSVLDFGETAWGKTAAEM
                     LRDRFRSTGKVQVADADLSRSAAMGVGYSGSLNLSVSEARDLGAALATDYYLIGDAQT
                     LRRSSSVSPVYYESYCSVFLVSSRSGRLLRWDRPSFAANNATKAEELLAENLSNKLYQ
                     ALLIAVARAEEDEQIQRTLITSSSTPLIEEAPDDEKTAAAQGLRLPRPYRRLRPEYPE
                     SAARAEAEATVDVLVDVGADGEVGEVQVVRWAGFGLDEATISTIKGMHFFPAMRNGTA
                     IPMRVLLRYNFRKPPR"
     gene            complement(3865..>5094)
                     /locus_tag="VFO72_10425"
     CDS             complement(3865..>5094)
                     /locus_tag="VFO72_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9479751.1"
                     /translation="RLIDAAEFQRLRRIKQLGLGLYTYQGAEHSRFTHSLGAFHLMTR
                     VLDRFGERYSIAPADRVAARAAALLHDVGHGSFSHVMEKLTNFHHEQRTVQVILDDAF
                     EIGALLKSYAPDLPRKIAAIIEGTFTPSALGQLVSSQLDVDRMDYLLRDSLMTGAKYG
                     IYDLEWIINALAIDEAADRIYVEARGVYAVEEYLQARYYMFRQVYFHRTLRSAEAALR
                     SIIRRALRLLDEGQEVWHAPGTAFEKILRREELTIAEHLQIDDSDFVFHVKQWQNSAD
                     AILSDLSRRFTARRLFKAIDLDMPQEQQAEFLMAARTIVEEAGFDPEYYFVEDRASDL
                     PYYNYYAAQKAEPKTHIYVESGYATPQIREISEVSNVVRGLQQPYELHRICFPAEVKA
                     EVYELYHRGGAKKRTVG"
BASE COUNT         1062 a         1518 c         1444 g         1070 t
ORIGIN      
        1 accggcatca ttgccggcgt tggcggtgat catgccggga ccgagaatcg cgaggtaagt
       61 gaacagacga ggacggcgcg ccactgtacg gcgcacgcca tgagcgccac gcattattcg
      121 gcgccctgca gctcgcactg ccgctcgtgt ttctgccact tggaggttct ccgtcgttca
      181 tctcacgttt ctttcccgtg cgtctctttc ccgtcccggg cgtctcttcc ctgggaccgg
      241 gcgcgctctt tcctgggacc gcaggcgtct cgcctgcacg gcgttagaag ccctctgcgc
      301 agcctggcag gcgagacgcc tgcggcggtc ccagggaaga gacgcctgcg gtctcaaaga
      361 gacgcctgcg gtcccaggga aggtttcaac cgagtagtct cggcagacgt tgccgccaat
      421 ccttcggcaa gaggatctcc atggcatcgt ctaccgtaat gatgccgagt atatctcctg
      481 actcgtcgat cacgggaagc gcaagcaggt tatattcggc gatgcggtgc gccacttcgc
      541 gagcaggctc atgcggatgc gcttgttgaa aatctgtgcg catcacgttt tccagcggca
      601 tcgacggatc cgccagaatc agactgcgca atgcgatgat gcccgacagc ttccacgaat
      661 tctcctcctc aacaacgtag aggtaataga tcatattcgg cgtctccgcc atctcgcgca
      721 gccgtgcaag cgcttcgccg actgtcaact gccgtggaag cgtaacaaac tcggtcgtca
      781 tcaaaccgcc cgctgtgtcg tcttcgtaag gaagcagttc agcgacctcg gcttgttctt
      841 cgtcttccat cagaccgagt agctcttccg ctttctcttc cggcagatcg ccaaggacgt
      901 cggccgcttc atcaggcgac atccattcga gaatgtcggc cgcgcgctcc tggtccatgt
      961 cgccgagaat tcttgcctgg tgttcggcgg gcatctcttc gagtgtctcg gcggcggttt
     1021 cgtcgtccag cgactcgacc acttcggagc catggtgata cgacagcgct tcggcaagtc
     1081 gcgcgatctc aaccggatgc aggcgcgcga gtttgtcgct cgaggatttc aactggaccg
     1141 tggccgcgtc cgtggccaga tagccaacgt cggcccagtc gagcacttcg acgggtttgc
     1201 gagtaccggc gaaaccggcg ggcgcaagac gtcgccacaa tccttgcaga ctcacgtctg
     1261 cgccggtgac gcgccactcg tctgcggcgt gaatgatctg cacgtcgttg actcgcacga
     1321 cgcgcttgcc gtcgacgtcg atcagttgtt tatcgagaac gtcgcgcgcg agtaagactt
     1381 cgctttcgcg ccgcacaaaa ggacgcagat cgagaatatc ggaattgagt cgcacgccat
     1441 gctcgtcgaa ttcggtgata cgccagcgtg agagaaagaa gtcgcgccgg cgataacgcg
     1501 caacgagacc cgtgacgggc ggatgatcgt cagccccgag gcgcacgatc acatccttga
     1561 ccgtggccac acgttcatct tccagatcgc gtatcgggcg acctaacact tgcgacaagt
     1621 aaagcacgcg ccaatcttac gcttaaagcg gtgattacga gtagcgaaag gttcgttaaa
     1681 gcgtggattt caccggccgt tcgagaaagt ttcgggcggc ttcgagggcg ggatcgcggc
     1741 gggtgtagag atcggaacgg gcgagcggcg cgagttgatc gggagtgacg ccgcggccct
     1801 gcaagcggat gccgcctgcc gtgcggtagt caaactcgct gacgtccaac aatccgccgt
     1861 caggcaacgc gtgtctgttt ctgatcgcca gcacgcaccc acacgtggcg gtgccgatca
     1921 cgcgggctcg gcttctggcc tgcaacgcgg ccgccataat ctctgctgcg ctcgacgtgc
     1981 tctcgctggt gaggacgacc agcgggagtt tgatctgaga taaccgcggc acacgccaga
     2041 gtcgttttga ataggtttgc agctcaaacg atgcgccgga gcgatccgcg aacttgccga
     2101 gattgacacc atcgacaaga aacagcgacg cgacgtcagc cattgcttca gcatcgccac
     2161 cgccattcgc acgcaggtcc agcacgatgc cttccgcgcc ttcgagcgcc ggcgccagcg
     2221 ctctggaaaa ctcgagcgcc agactctggg tgaacgcgtc gatttttaaa attgcgagtt
     2281 ttccttttct ttgtgtcgtg aacccgagct gccgctggct ccagtaacgc tgcaacaccg
     2341 ccgacttcag ttttccttct cgtgtggccc acgcgacctt cactgagctg ccggatagac
     2401 catcaaagag actggcgatc gatcggtagc gcgctcgttg atccgcgccg aagttctcca
     2461 gacgctcttt gataaactcc gccaccggca gatcatcgat gctcacaatc gcgtctccga
     2521 cacggatgtc ggttcgcgcg gccgcagagt ttggttccac ctgcacaact accggaacac
     2581 cgtcaacctc gcgaacgcta acgcccaccg taatgaagcg cgggttccac cagtcaaact
     2641 tttcatcggg agaatagacg cgcgtatgtg cgtcccgcag agacgcgatc atctgtcgca
     2701 tcacttcgta aaactcgtgc gtgttcgcgg cctttgccgc ggccggtcga aaccaggtgc
     2761 gcttggcgtg ccaatcgacg ccattgaatc gcggatcgta gtagcggtcc tggatcgtct
     2821 cccaaacgtc gtcaaaaaca acgagacgtc cttcggccgt agtagtcgaa actagcgaag
     2881 ggtcgtccga tgccccgtgc gaactgatcg tcagttgtga aacgaaaagg cagaggactg
     2941 cgaggagcag gagaaccttg gaagaatgtc ttgaggaacg atccattcac tacctcgggg
     3001 gcttgcgaaa gttgtagcgc agcagcacac gcatcgggat cgcagttccg tttctcattg
     3061 cgggaaagaa gtgcataccc ttaatcgttg aaatcgtagc ttcatcgagt ccgaaaccgg
     3121 cccaacgaac gacctgcact tcaccgactt caccatccgc gcctacgtca accagaacgt
     3181 ctacggtcgc ttcggcttcg gcccgcgcgg cgctttccgg gtactccggt cgcagccgtc
     3241 gatacggtcg cggcaagcgc agaccttgag ctgcggctgt cttttcatcg tcgggtgctt
     3301 cttcaatcaa cggcgtcgaa gacgacgtga taagtgtccg ttgaatctgt tcgtcttcct
     3361 ctgcgcgcgc aaccgcgatc agcaacgcct ggtagagttt gttggaaaga ttctctgcca
     3421 gcagttcttc agctttggta gcgttgttgg ctgcaaagct gggtctatcc caccgcagca
     3481 ggcggccgct tctcgaactt accaggaaca ctgagcagta ggactcataa tatacgggag
     3541 agacggatga tgagcgccgc aaagtctgtg cgtcgccgat taaataatag tccgtcgcca
     3601 gcgctgcgcc caaatcacgt gcttcgctga cgctcagatt gagcgaaccc gagtaaccca
     3661 cacccatcgc agccgagcgc gacaagtcag catctgcgac ttgtaccttt ccggtagatc
     3721 gaaaccggtc tcgcaacatc tcagcagcag tcttgcccca cgcggtttcg ccgaagtcca
     3781 acacactcaa cgttggcttg ggactttgcg cgtgcgcagt ttcgaggaaa aaaagcaatg
     3841 cacagatggc acagaaagca cgaatcatcc gaccgttcgt ttcttcgcgc cgccgcgatg
     3901 ataaagttcg tacacttcgg ccttgacctc agccggaaaa cagattcgat gcagctcgta
     3961 cggctgttgc agtcctctca ctacattgct gacttcactt atctcgcgga tctgcggcgt
     4021 cgcataaccg ctttccacgt agatgtgcgt cttgggttcc gccttttgcg ccgcgtagta
     4081 gttgtaataa ggcagatcgc ttgcgcggtc ttcaacgaag tagtattcgg gatcgaaacc
     4141 agcctcctcg acgatcgttc ttgcggccat gagaaactcc gcctgctgct cctgtggcat
     4201 atcgagatcg atcgctttga aaagacgccg cgcggtaaac ctgcgactga gatcactcaa
     4261 gatcgcgtcc gcggagtttt gccattgctt cacgtggaag acgaagtccg aatcgtcaat
     4321 ctgaagatgc tcggcaatcg tcaactcttc gcgacgcaga atcttttcga aagccgtgcc
     4381 gggcgcgtgc caaacctcct ggccttcgtc gagtagtcgt agcgcccgtc gaatgatcga
     4441 acgcagcgcg gcttcggcgg atcgcagcgt gcgatgaaag tagacctggc gaaacatgta
     4501 gtaacgcgcc tgcaaatatt cttcgacggc atacacgcca cgtgcttcta catagatccg
     4561 atccgcggct tcgtcgatcg ccagggcatt gatgatccac tcgagatcgt aaatgccata
     4621 cttcgccccg gtcatgagtg agtcgcgcaa caggtagtcc atgcgatcga cgtcgagttg
     4681 cgacgacaca agttggccca aggcagatgg tgtaaacgtg ccttcgataa tggctgcaat
     4741 ctttcgtggc aggtccggtg cataagattt caagagcgcg ccgatttcga aagcgtcgtc
     4801 gagaattacc tgcacggtcc gttgctcgtg atggaaattg gtcaacttct ccatcacgtg
     4861 agaaaacgag ccatggccca cgtcgtgaag taaggccgca gcgcgcgcgg ctacacgatc
     4921 ggcaggagcg atcgaatatc tctcgccgaa gcgatcgagc acgcgcgtca ttagatgaaa
     4981 tgcgccgagt gaatgagtga agcgagaatg ttcggcgcct tgatacgtgt atagaccaag
     5041 accgagttgc ttgatgcgac gcaggcgttg aaactccgcc gcatcgatga ggcg
//
LOCUS       DASTEY010000595         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_985516, whole genome shotgun sequence.
ACCESSION   DASTEY010000595 DASTEY010000000
VERSION     DASTEY010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985516"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFO72_10430"
     CDS             <1..1145
                     /locus_tag="VFO72_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET9479752.1"
                     /translation="ELNNSLARLSRFADSFWKGFRDGRGEDGRYPIIPGTFARRNAGG
                     ELESTQLGELYLFFEGAMARVESTLDAIAEKPPEVESLIRRLRQIRFDLEFIVTGADK
                     KFVYWLERRNRGVFLRASPIDVAGLLQDKLFEEVPTVVLTSATLSSGGNFNFIRDRLG
                     LDTADDLIAESTFDYERQAILYLPPKMPDPRSNEWAAAAADEVLKILHATEGRAFVLS
                     TSFAGMQSLFQNVWAELEYPCLVQGSASKGQLLKQFRETPNAVLFATSSFWQGVDVRG
                     QQLSCVIVDKLPFAVPSDPLVAARQRYIDDSGGSSFYQYSVPQAIIALKQGLGRLIRS
                     TTDRGVLAVLDPRLRTKGYGRTFLQSLPPCRVTSRIDELSSILVSD"
     gene            1148..1528
                     /gene="queD"
                     /locus_tag="VFO72_10435"
     CDS             1148..1528
                     /gene="queD"
                     /locus_tag="VFO72_10435"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET9479753.1"
                     /translation="MPGQFEVMIERNFSSAHQLRGYKGKCENLHGHNYKIEIYARGRE
                     LDNIGLLVDFVDLKAAADEVVTYLDHRNINELPPFDELQPSAENLAKYVLERVSSRIS
                     DDRVQIYKVRCFETPTSVATYQLV"
     gene            complement(1534..>2195)
                     /locus_tag="VFO72_10440"
     CDS             complement(1534..>2195)
                     /locus_tag="VFO72_10440"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9479754.1"
                     /translation="MDAKFLPDAESANVIAELKGSEKPDEIVILAGHYDSWDVGQGAH
                     DDGGGCIVAWEAARLLKELGLRPRRTIRVVLYTNEENGLRGGNAYRDAHRAELSKHIF
                     AIESDSGVFRPEGLGLAATAPPMVRSNLQEIAKLLSGIGADRIAPGGGGADIGPIMRE
                     GVVGASLDVEGSRYFDIHHTPADTFDKINSRELALCVATMAVMAYTIADLPEPLTGTA
                     N"
BASE COUNT          448 a          641 c          629 g          477 t
ORIGIN      
        1 gcgagttgaa caacagtctc gcgcgcttat cgcgcttcgc ggatagcttc tggaagggct
       61 ttcgcgacgg acgcggcgaa gatggtcgtt acccaatcat tcctggcacc ttcgcgcgaa
      121 gaaatgccgg cggcgagctc gaatcgactc aactcggcga actctacctg ttcttcgagg
      181 gcgcgatggc tcgcgtcgaa agcacgctcg acgcgatcgc cgagaaacca cccgaggtcg
      241 agagcctcat tcggcgtctg cgtcaaatac gtttcgatct cgaattcatt gtgactggcg
      301 ctgacaagaa gtttgtctac tggctggagc gccgcaaccg cggagtgttt ctgcgtgctt
      361 cgccgatcga tgtcgcgggt ctgctgcaag acaagttgtt tgaagaagtc ccgaccgtag
      421 tgctcacgtc cgcgacgctt tccagtggtg gtaactttaa cttcattcgc gatcgactcg
      481 ggctggacac tgccgacgat ctcatcgctg agtcgacttt cgactacgag cgccaggcga
      541 ttctttacct gccgccaaag atgcccgatc cgcgctcgaa cgagtgggct gccgccgcgg
      601 cggacgaagt cttgaagatc ctgcacgcaa ccgaagggcg tgcgtttgtt ttgtcgacca
      661 gcttcgcggg catgcagtcg ttgtttcaga acgtctgggc cgagcttgag tatccgtgtc
      721 tggtacaagg cagcgcttcg aaggggcagt tgctgaagca gtttcgcgag acgccaaatg
      781 ctgtgttgtt tgccacgtcc agtttctggc agggcgtcga tgtgcgaggg cagcagttgt
      841 cgtgtgtgat cgtcgacaaa ctgccgttcg ccgttccgag cgatccgttg gtagccgcgc
      901 ggcaacgcta catcgacgac agcggtggtt cgagttttta ccagtacagc gtgccgcagg
      961 cgatcatcgc gttgaagcaa ggcctcggtc gcctgattcg cagcactacg gatcgcggcg
     1021 ttttggccgt gctcgacccg cgtttgcgca cgaaaggtta cggccgcacg tttctgcaaa
     1081 gtctgccacc ctgccgcgtc acttcgcgca tcgacgagct atcgagtatt ttagtgtcgg
     1141 attaatcatg cctggacaat tcgaagtaat gatcgagcgg aacttctcga gcgcgcatca
     1201 attgcgcggc tacaaaggga agtgtgaaaa cctgcacggc cacaactaca agattgaaat
     1261 ctacgcgcga ggccgcgagt tggataacat cggtttgctg gttgattttg tagatttgaa
     1321 agcggcggcg gacgaggtag tgacgtacct ggaccatcga aacatcaacg agttgccgcc
     1381 gtttgacgag ttacagccgt cggcggagaa cctggcgaag tatgtgctgg aacgagtgtc
     1441 gtcgcgcatc agtgacgacc gcgtgcagat ctacaaagtt cgctgtttcg aaacaccgac
     1501 cagcgttgcc acctaccaac tggtctgacg cgtctagttc gcggttccag tcagcggttc
     1561 cggcaggtcc gcaattgtat atgccatcac tgccatcgtc gccacacaca gcgccagttc
     1621 tcgcgaattg atcttatcga acgtgtcagc gggcgtgtga tgaatgtcga aatagcgcga
     1681 accttcgaca tcgaggcttg cgcccaccac gccttcacgc atgattggac caatgtccgc
     1741 cccgccgccg cccggcgcaa tgcgatcggc gccgatgccg gaaagcagct tcgcaatctc
     1801 ctgaagatta gaccgaacca tcggaggcgc tgtcgctgcg agacccaaac cctccggacg
     1861 aaacacaccc gagtcagact cgatcgcgaa gatgtgtttt gaaagttcgg cccgatgcgc
     1921 gtctcgatac gcgttgccgc cacgcagtcc gttttcttcg tttgtgtaaa gcactacccg
     1981 aattgtgcgg cgtggacgca atcccaactc cttgagcaag cgagcggctt cccacgcaac
     2041 gatgcaaccg ccaccatcgt cgtgcgcgcc ctggcccacg tcccacgaat cgtagtgtcc
     2101 ggcgaggatt acgatctcgt caggtttctc cgagcctttc aactcggcaa taacattcgc
     2161 cgactccgca tccggcaaaa acttcgcgtc catct
//
LOCUS       DASTEY010000596         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_969413, whole genome shotgun sequence.
ACCESSION   DASTEY010000596 DASTEY010000000
VERSION     DASTEY010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969413"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..804)
                     /locus_tag="VFO72_10445"
     CDS             complement(97..804)
                     /locus_tag="VFO72_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9479755.1"
                     /translation="MSAVLIVEDEKHLADGLRYNLEAEGYEVEVAGSAEEAYTLLTDR
                     QPAFDVIVLDVMLPGMDGFTLASRLRAQGHFIPILMLTARGQAQDVLRGFEAGADDYL
                     PKPFDLSVLLARINALLRRREWFQKDVSKSNDGVFAVGNKTIDFEALELRSAEQTVQL
                     TLMEAELLRYLINHEGKIVSRSAILEDVWGLREDTDTRAIDNFIVRLRKYLEDEPSRP
                     RHLLTVRGVGYRFISQG"
     gene            complement(809..1711)
                     /locus_tag="VFO72_10450"
     CDS             complement(809..1711)
                     /locus_tag="VFO72_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9479756.1"
                     /translation="MRIKPRRRKSVAFFITLGACLVALAVALSVGWIILNWREVALLV
                     LGIVFFNLLIFGLVINTTFLIREIRRNEQHDAFINAVTHELKTPVASIRLYLETLKTR
                     EVDEQQRQEFYNIMLADSDRLLKTIEQVLRASRTRHRQRRIAMSVINLSEMVRECLEL
                     ARVQYGLSETHLIYTESPEASNARVSGDIDELRAAFANLLDNAVKYSDEQVRVVVSVS
                     SPDDKRVTVRVADQGIGIPQSQLRRIFKRFYRVPGRFMSRVKGTGLGLFIVESVVQKH
                     GGRVYAESAGPGHGSTFTVQLPRV"
     gene            complement(1731..>3075)
                     /locus_tag="VFO72_10455"
     CDS             complement(1731..>3075)
                     /locus_tag="VFO72_10455"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9479757.1"
                     /translation="NDINRQSKIENQKYYMDSLTYTQLLRRNRSFRRLWIGQVISELG
                     NWFNFIAVLGLVRVVSNAAPEVTTIVLIVRLVPFTLFAPLAGAFVDRWSRRKVMIATD
                     LLRVAVALGPLFIRRPQDLWIAYVCTALLSFFGTFFEAAKNAAVPNITGERDLLAGNA
                     LMFSSRFLLMSLGAALGGWTAAHVGYSAAFIINALSFFASAYSVWLVPEEETKQRSIE
                     TAQSQPKPRYTGYWSDIREGWSYIVTHAPVATILGINIIWAVGGGASNLIADRLGALV
                     FAGENGISPDSAVAALYFANGMGLFLGMLIARRVGAYFELKQRTIGFIGWSLLAQGIV
                     FGLSGVMPALWLACALLLVSRVMLGAEFAVQETLLMRLVPDNLRGRVSTTDRAGEMLI
                     WTFSTGIAGWSLRMITPRTLTVIAGFLSGLAGIVWLVLFASQAVRLPRRLVHRLD"
BASE COUNT          717 a          908 c          840 g          610 t
ORIGIN      
        1 gcaaagagag ccgttaagaa acgcggtagc actttgcgcc tttgcgcctt tgcgcgagac
       61 atttcttgcg atagaggtac gttcgggcaa agccgttcac ccctgcgaaa taaaccggta
      121 gccaactccg cgaaccgtca aaagatggcg cggccgcgaa ggttcgtcct cgagatactt
      181 gcgcagccgc acgataaagt tgtcgatcgc ccttgtgtcc gtgtcttcac gcaggcccca
      241 gacgtcttcg agtatcgcgc tacgcgagac tatttttcct tcatgattga tcaggtagcg
      301 aagcagttca gcttccatca gtgtgagctg cacggtttgt tccgccgagc gcagttccaa
      361 cgcttcgaaa tcgatggtct tgtttccgac ggcaaagacg ccgtcattac ttttgctgac
      421 atccttttga aaccactcgc gccggcgcaa caaagcattg attcgcgcca gcaacactga
      481 caggtcgaac ggcttcggca agtagtcgtc cgcgccggct tcaaaaccgc gcagcacatc
      541 ctgcgcctgg ccgcgcgcag tcagcatcag gatcggaata aagtgtccct gggcgcgtaa
      601 tctcgacgca agcgtgaagc catccattcc gggcagcatc acgtcgagca caataacgtc
      661 aaaggcgggc tggcggtccg tgagcagcgt atacgcctct tccgcgctgc cggcaacctc
      721 gacctcgtaa ccctcagctt cgaggttgta gcgcaggcca tcagccagat gtttttcgtc
      781 ttcgacgatc agaacggcac tcattgagtc acactcgcgg aagttgaact gtaaacgtgc
      841 tgccatgacc cgggcccgcg ctctcggcgt acacgcgccc accatgtttc tggacgactg
      901 actcaacgat gaacaatccc agtcctgttc ctttgacgcg tgacataaac ctgccaggca
      961 ctcgatagaa cctcttgaag atgcgtctga gttgtgattg cggaatccca atgccctggt
     1021 cggcaacgcg caccgtcacg cgtttgtcgt caggagacga aaccgacacg accacgcgca
     1081 cttgttcgtc ggaatacttc accgcgttgt cgagcagatt tgcgaacgcc gctcgcagtt
     1141 cgtcgatgtc gccggagacg cgtgcattac ttgcctcggg cgattccgtg tatatcaaat
     1201 gtgtttcgct caagccgtac tgaacacgcg ccagttccag gcactcccgc accatctcac
     1261 tgagattaat caccgacatg gcaatgcgcc gttggcggtg acgtgttctg ctcgctcgca
     1321 gcacttgctc gattgtcttc agcagtcgat cgctgtcggc gagcatgatg ttgtaaaact
     1381 cctgtcgctg ctgttcgtcg acctcacgtg ttttgagtgt ttccagatac aaccggatgg
     1441 aagcaacagg cgttttcagt tcgtgagtga cggcgttgat gaacgcgtcg tgctgctcgt
     1501 tacggcgtat ctcgcgaatc aggaaggtcg tgttgataac cagtccgaaa ataagcaggt
     1561 tgaaaaagac gatccccaaa acgagcagcg cgacctcgcg ccagttgagg atgatccagc
     1621 ccacactcaa cgccaccgcg agcgcaacca ggcacgcgcc caacgtgatg aagaacgcga
     1681 cggatttgcg gcgacgaggc tttatacgca tcgcggtgat tgtagcaaca tcagtctagt
     1741 ctgtggacca accggcgcgg caggcgcacc gcttgagacg caaacaacac gagccaaacg
     1801 attcccgcca atcccgagag gaaacctgct atcactgtaa gcgttcgtgg cgtgatcatg
     1861 cgtaacgacc atcccgcgat tccggttgag aacgtccaga ttaacatctc gcccgcacga
     1921 tcagtcgttg acacacggcc acgcaaattg tccggcacca gacgcatcaa gagcgtctcc
     1981 tgcaccgcaa actccgcgcc gagcatcact cgtgagacga gcagcagcgc acacgccaac
     2041 cacagcgccg gcatcacgcc cgacaaaccg aagacgattc cctgcgccaa cagactccag
     2101 ccgatgaagc cgatcgtccg ctgcttgagt tcaaagtagg cgccgacgcg acgcgcgatt
     2161 agcatgccga gaaacagacc cataccgttt gcgaaataca acgcggcgac cgcagagtca
     2221 ggactgattc cgttttcacc agcaaagacc aaggcgccga gacgatcggc gatgaggttg
     2281 cttgcgccac cgccgacagc ccagatgatg ttgataccta gaatcgtggc gaccggagcg
     2341 tgcgtaacga tgtaggacca gccttcgcga atgtccgacc agtaacctgt atagcgtggt
     2401 ttcggttgtg actgcgcggt ctcgatcgat cgttgctttg tctcttcttc aggcacgagc
     2461 cagacggaat acgccgacgc gaaaaacgag agcgcgttaa tgatgaaagc ggccgaatag
     2521 ccgacgtgcg cggcggtcca gccaccaagc gcagccccaa gcgacatcaa caaaaagcgc
     2581 gatgagaaca tcagcgcgtt gcctgcgagc agatctcgtt cgccggtgat gtttggcacg
     2641 gccgcgtttt tcgcggcttc gaagaaagtg ccgaagaacg aaagcaaagc ggtgcagacg
     2701 taggcgatcc acagatcctg cgggcggcgt ataaacaacg gtccaagtgc gactgccacg
     2761 cgcagcagat cggtcgcgat catcactttg cgacgcgacc atcggtccac gaatgcgccg
     2821 gcaagcgggg caaacagcgt gaacggcacc aagcggacga tcagaacgat cgtggtgacc
     2881 tccggcgctg cgttggacac aacccggacc aatccgagca cggcaataaa gttgaaccaa
     2941 ttgccgagtt cggaaatgac ctgtccgatc cacaatcgcc ggaacgagcg attgcgacgg
     3001 agtagttgtg tgtaagttaa cgaatccatg tagtattttt gattttcgat tttcgattgc
     3061 cgattgatgt cattg
//
LOCUS       DASTEY010000597         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1431021, whole genome shotgun sequence.
ACCESSION   DASTEY010000597 DASTEY010000000
VERSION     DASTEY010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431021"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="VFO72_10460"
     CDS             <1..1921
                     /locus_tag="VFO72_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479758.1"
                     /translation="DFSTTRVEMPLDSSAACVRVIHDAQSLWISMQEDESSRSLFADM
                     QWQDDVLAVPLFGISEIPAKPGRDARRNQYWTFEPGARLGVLYVGANRDTVDPDSLIL
                     IKRFAERIGIVASLATHQERLVNTVTKLQRERQWIESIMKSVADPIVLTDLDNEILLQ
                     NRRAEELFSGSANAGEGKRRALKMNDLLFSAYLSSATVSSSEVIQRDITLVDPIEGSD
                     IHFEVISTPAANARGEPLGLVSIFRDVTDLREANDELAHNFGKLQHAESESRRERDRL
                     DLIIENVGHPIVVCDSAGNFILFNRRAELLFQENASFRSSAAAAVRANAVKLTSFISG
                     LASASETGRQAEIELIDPESGRSLPMEITAREVLDATGQVTAVVSILHDLTEIRELER
                     RRVEQQLFESEKLAAVGRLAASIAHEVNNPLEAIKNALYLMQTNAGEDEKNLRFLEIA
                     RKETERVSHIIRQMLGFARRPGEVDWVDVNQLLEETLVLMEKKLRQLRIRITRSFDDS
                     LPKIRARADQLRQVFLNLIINAQQAIQGDGEIVISTSRYEQALQPSIVIQLSDSGVGI
                     SEDDLNRIFDPFFSTGKKGTGLGLWVTQDIVRQHGGRIEVSSEVGRGTVFSIVLQVDS
                     PILEDVKSESLSSSK"
     gene            1918..3579
                     /locus_tag="VFO72_10465"
     CDS             1918..3579
                     /locus_tag="VFO72_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9479759.1"
                     /translation="MSHLFDNHGNGAPLEDASVFVPVLNEARLLVVDDEESLRITTAA
                     IFEKEGYIVDTASSGDEAIDLLGKTDYDLVLTDLHMEGGDGLSVLNQIRRHAPLTISV
                     VLTGFASVESAIAALQEGAYDYLIKPCDIESMKHTIRRGVEHRRLMLAEQKARADLQQ
                     LNRDLEQRIEERTAELTRLNAELAEANRAKDVFLATLSHELRTPLTPVVGWIKLLRSG
                     TLDEKSVSQALDAIERNAWLQSRLIDDLLDTSRIATGKLHFEPKPTDLNVVVKAAVDT
                     VRNSAASRNIDLSMSLHPAGLVVMGEPVRLQQIAWNLVSNAIKFTDPGGKVTVTTGLN
                     GNQAYITVVDTGVGIPGEFLPHVFDRFRQAVGSTSRRHGGLGLGLAIADALAKLHGGR
                     LEAHSDGEGQGAIFTLRVELASTGSVVAETVHERVHSLEGLDILIVEDSPDTLLLLST
                     MFSRGGATVTTAASASEALQSAVVKRPHIIVSDIGMPETDGYQLLEQLRLVPGLSDVP
                     AIAISGYASEEDRERALSVGYLALVPKPVDVDTLFTLIQDLKTPR"
     gene            3726..4274
                     /locus_tag="VFO72_10470"
     CDS             3726..4274
                     /locus_tag="VFO72_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9479760.1"
                     /translation="MSTNSRFAVAVHVLSLMAWSGEEPLKSEQVAESVNTNPVVIRRM
                     LKELAEAGLVVSQTGSLGGSRLANDPAKTTLLDVYQALECRGVFSLHRQPPSRDCPVG
                     VNIETVLGDVLLEVDSAVERVLENITINDVVQRLKPCGGNATSKTMEKNGRKKHFDEK
                     QIALPALRAGAKSRRARAFRTA"
     gene            4302..>4587
                     /locus_tag="VFO72_10475"
     CDS             4302..>4587
                     /locus_tag="VFO72_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479761.1"
                     /translation="MARLEKDQSIELMNSGPGEFVNLSEELTDDAVSIEDIDEVLQTL
                     ERATSSETEIEEPLIEESSSAVESDESMSEEVELDLSAGEMDKTSDPIRVY"
BASE COUNT         1043 a         1184 c         1349 g         1011 t
ORIGIN      
        1 ggacttttcg acaacacgag ttgagatgcc actcgattcg agtgctgcct gcgtgcgcgt
       61 gatccacgat gcacagtcgt tatggatctc gatgcaggag gatgaatcgt cacgcagtct
      121 tttcgcggac atgcagtggc aggatgatgt tctggcggtg cctcttttcg gcatttcaga
      181 gattcctgcg aaacccggtc gcgacgcgag gcgcaaccag tactggactt ttgagcccgg
      241 tgcgcggttg ggcgtgcttt acgtcggagc gaatcgcgat acagtcgacc ctgactcttt
      301 gattctcatc aaacgattcg cagagcggat tggtatcgta gcttctctgg cgacgcacca
      361 ggaacggctc gtcaataccg tcaccaaact ccagcgtgag cgacagtgga tcgaatcaat
      421 catgaagtcg gttgccgatc cgatcgtgct caccgatctc gacaacgaaa tactgctgca
      481 gaatcgtcgt gctgaagaac tctttagcgg ctccgcaaat gccggtgaag gaaaacggcg
      541 tgcgctgaag atgaacgacc tgctgttctc ggcttatctt tcgagcgcca cggtttcctc
      601 gtcagaagta attcaaagag acatcacgct ggtcgatccg atcgaaggtt ctgacattca
      661 cttcgaagtg atttcgacac cggcggcgaa cgcgcgcggc gagccactgg gactggtgtc
      721 gatctttcgc gacgtaactg acctgcgtga agcgaacgac gagttggcgc ataacttcgg
      781 taaactgcaa cacgcagaat ccgagtcacg acgcgaacgc gatcgtctcg acttgatcat
      841 cgaaaacgtg ggccatccga tcgtggtgtg tgattcggcg ggtaacttca ttctgttcaa
      901 ccggcgcgcg gagttgttgt ttcaggagaa tgcatctttc cgatcgtccg cggcggccgc
      961 agtgcgtgcc aacgcagtca aactcacttc gtttatttcc ggtctggcgt cggcttcaga
     1021 aacaggcagg caggccgaga tcgagttgat tgatcctgag agtggcagat cgttgccgat
     1081 ggagatcacg gcgcgtgaag tgttggacgc gactggccag gtgacggctg tagtttccat
     1141 tcttcacgat ctgactgaga ttcgagagct cgagcgacgt cgcgtcgaac aacagttgtt
     1201 cgaatcggag aagcttgcgg cggtcggacg tctcgcagcc tctatcgctc acgaagtcaa
     1261 caatccgctc gaagcgatca agaatgcgct ctacttgatg caaacaaatg ctggtgaaga
     1321 cgagaaaaac ttgcgctttc tcgagatcgc gagaaaagag accgagcgag tttcgcacat
     1381 cattcgccag atgctcggct ttgcgcggcg gccgggcgaa gtcgactggg ttgacgtgaa
     1441 tcagttgctg gaagagacgc tcgtgctgat ggaaaagaag ttgcgccaac tgcgcattcg
     1501 catcacgcga tcgtttgacg attcgctgcc aaaaattcga gcccgcgccg atcagttgcg
     1561 ccaggtgttt ctcaatctga ttatcaacgc gcagcaggcg attcaaggtg atggagagat
     1621 cgtgatttcc acgtcacgtt acgaacaggc gttgcagcct tcaattgtga tccaactcag
     1681 cgacagcggc gttggcattt ccgaagacga tctcaatcgg atattcgatc cgttcttttc
     1741 gaccggcaag aaaggaacgg gcttgggatt gtgggtgacg caggacatcg tcaggcagca
     1801 cggtggacgc atcgaagtaa gtagtgaagt gggtcgcggg acggttttca gcattgtgct
     1861 tcaggttgac tcgcccatac tggaggatgt gaagagcgag tctttatcat cgtcaaaatg
     1921 agtcatttat ttgacaatca tggaaacggt gcgccgctcg aagatgcgag tgtgttcgtt
     1981 ccggtgttaa acgaagcgcg actgctggtc gtcgacgacg aagagagtct gcgcatcact
     2041 accgctgcga tttttgaaaa ggaaggttat atcgtcgata ccgcgtcgag tggtgacgaa
     2101 gccatcgatc tactcggtaa gactgattac gatctggtgc tcaccgacct gcacatggaa
     2161 ggtggcgacg gtctttccgt tctgaatcag atccggcgtc atgcgccctt gacgatctcg
     2221 gtggtgctga ccggatttgc gtctgtggaa tccgccatcg cggctttgca ggaaggcgct
     2281 tacgactacc tcatcaaacc ctgcgatatc gaaagcatga agcacacgat tcgtcgtggc
     2341 gttgaacacc ggcgactcat gctggcggaa cagaaagcgc gcgcggacct gcaacagttg
     2401 aatcgcgatc tggaacagag aatcgaagaa cggaccgcgg agttgacacg tctaaatgcg
     2461 gagctcgccg aagcgaaccg cgccaaggat gtcttccttg ccacgctctc gcacgagttg
     2521 cgcactccgt tgacgcccgt ggtcggatgg atcaagctgc tgcgcagcgg cacgctcgat
     2581 gagaagagcg tttcacaggc actcgacgcc atcgaacgta acgcgtggct gcagtcacga
     2641 ctgatcgacg atcttctgga tacctctcgc attgcgaccg gaaagctgca ttttgagccc
     2701 aagccaacgg atctgaacgt tgttgttaaa gcggcggtcg atacggtgcg caattccgca
     2761 gcttcgcgga acatcgattt gtcgatgtcg cttcatcctg cgggtctcgt tgtgatgggc
     2821 gagccggtgc gtctgcaaca gattgcgtgg aacctggttt cgaatgcgat caagtttacc
     2881 gatcccggtg gcaaggtgac tgtgaccact ggactgaatg gaaaccaggc ttacatcacg
     2941 gtggtggata ctggtgtcgg gatcccgggg gagtttttgc ctcacgtttt cgatcgcttt
     3001 cgacaggccg tcggttcgac gtcgaggcgt cacggtgggt tggggctcgg actcgcgatt
     3061 gcggacgcgt tggcaaagtt gcatggcggc cggctcgaag cacattctga tggagaggga
     3121 cagggagcga tcttcacctt gcgagtcgag ctagcatcaa ccgggagtgt agttgcggag
     3181 actgtgcatg aaagggttca ttctctggaa ggcctggata ttttgatcgt cgaagactcg
     3241 cccgacacgc tgttgctctt gagcacgatg ttcagtcgcg gaggcgcgac tgtgacgacc
     3301 gccgcttcag cctccgaggc tctgcaaagt gccgtcgtga aacgcccgca catcatcgtt
     3361 tcggacatcg gcatgccgga aaccgacggc tatcaactcc tcgaacagct acgcctcgtc
     3421 cccggcctga gcgacgtccc cgccatcgcc atttccggct atgccagcga agaagaccgc
     3481 gagcgcgcgc tctccgtcgg ttacctcgcc ctcgttccca aacccgtcga cgtcgacacc
     3541 ctcttcaccc tcatccagga tctcaaaacc ccgcgttgaa gtgaacggga tacttttagc
     3601 cgcagatttg cggctagtta atcgccggat tgcgtaaatt actgagtata aaacagttga
     3661 caaaatcaaa tctgtaacta taatggttac aaaaaggcgg ccggtggttc cccagatagt
     3721 ttgatatgtc gacaaatagc aggtttgcgg ttgcggtgca tgttttgagc ttgatggcct
     3781 ggtcaggcga agaacccctt aaatccgagc aggttgcgga aagcgtaaat actaatcccg
     3841 tcgtgatccg gcgcatgttg aaggaactgg ccgaggctgg gctggttgtg tctcagaccg
     3901 gttcgctggg cggctctcgg ctcgcaaatg accccgcaaa aacgaccctt ctggacgtct
     3961 accaggcact cgagtgccgc ggcgtcttct cgctgcaccg gcaacctccc agtcgcgact
     4021 gcccggtggg cgtgaatatt gaaactgttc taggtgacgt gctcctggag gtagattctg
     4081 cggttgaacg ggtacttgaa aatataacca ttaacgacgt cgtccaacgc ttaaaaccct
     4141 gcgggggtaa tgcgacatcg aagacgatgg agaaaaatgg aaggaagaaa catttcgacg
     4201 aaaagcagat tgctttgccc gcgttgcgag caggtgcaaa gagccggagg gcacgagcct
     4261 tccgaactgc gtaaataaac cgcgagaaag gaaagttaat tttggccagg cttgaaaaag
     4321 atcaaagcat cgagctcatg aactcgggac caggcgagtt tgtcaacctc agcgaggaac
     4381 tgaccgacga tgccgtgtcg atagaggata tcgacgaagt gctgcaaacg ctcgagagag
     4441 cgaccagtag tgaaaccgag atcgaggagc ctttgatcga ggagtcctcg tctgcggttg
     4501 aatctgatga atcgatgagc gaggaggttg agctcgacct ctcagccggc gagatggata
     4561 agacttcgga cccgattcgc gtttaca
//
LOCUS       DASTEY010000598         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_62839, whole genome shotgun sequence.
ACCESSION   DASTEY010000598 DASTEY010000000
VERSION     DASTEY010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62839"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..835
                     /locus_tag="VFO72_10480"
     CDS             71..835
                     /locus_tag="VFO72_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9479762.1"
                     /translation="MHLNRLTLVLIVCALAFVPVHAHQQTPHHTSKKSTKKEQQTYSC
                     PMHPDVTSKSKGKKCPKCGMDLRLVKHDEATIEATPVSHDVGATKMNIPDIELLDQNG
                     RKIHFYTDLVKGQTVVINFIFTTCTTICPPLGATFARVQKALGDKVGRDVRFISISVD
                     PATDTPERLKAWGAKFHAGEGWTFVTGNKPQVDELLRALGASSARREDHSPTVLIGND
                     AHGTWTRTYGLANTSKLVEIINEAIAGATTTAEGSK"
     gene            835..1527
                     /locus_tag="VFO72_10485"
     CDS             835..1527
                     /locus_tag="VFO72_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9479763.1"
                     /translation="MTRQVFILFILAVVVFVTTDNCLAQASAGGQTPGQVSTPPAKPA
                     APPAPHDHSHMTKTAEKSSVAANYFTDVELTNQDGKKVRFYSDVLKGKTVVVNAFFTT
                     CTSVCPPMNRNMEKIQEVFGDRVGRDVFLVSITVDPEVDTPARLKEYAKKFHAGPGWI
                     FLTGKKENLDWALYKLGQYVERKDDHKTIFIIGNEPTGLWKKAFGMANVAELVQVVES
                     VVNDKGEKGSTR"
     gene            1529..>2751
                     /locus_tag="VFO72_10490"
     CDS             1529..>2751
                     /locus_tag="VFO72_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9479764.1"
                     /translation="MNRKSKFFVGMLLAGFVLTLSGGLLASVAQQTPAPQQSSRSLTP
                     QERRGKALYMRGESASGKEITAILGDFDVPASTLNCAGCHGLRGEGKTEGGVTAGNLT
                     WSNMIKSYGHTHPSGRKHGAFDEKLFIRAMVQGVDPAGNELAKAMPRYQMSPEEIADL
                     IAYLKRIEADRDPGLTETAIKVGTILPKDGALAEIGAAMKDVLTAYFANVNEKGGIYN
                     RRIELQTIDTGTDAATTAANARKHVENGEVFALVSGLSAGADKELAAVVREDEIPFMG
                     PVTLLTQTSGQDNRNLFYLLPGAGQQARALVNFAAKKPELKKSQLAIVYSENELALAA
                     AAAIEDQARKLGWTSITRRSLSNDAAATVTALKAEGAESVFFLAAGGGEAEFINAAVS
                     ANWTPHVFLLGALTG"
BASE COUNT          671 a          755 c          786 g          539 t
ORIGIN      
        1 cgtggccacc ccagtcttcg acttcgtacg ctggggtggc cacggagggc cacccctaca
       61 aaggagaaac atgcatctta accgtctgac gcttgtattg atcgtttgcg cgctcgcgtt
      121 tgttcccgtg cacgcgcatc agcagacgcc gcaccacacc agtaagaagt caacgaagaa
      181 agagcagcaa acctattcgt gtcccatgca cccggatgtg acgtcgaagt cgaagggcaa
      241 gaagtgtccg aagtgcggca tggatctgcg cctcgtcaag catgatgagg cgacgatcga
      301 agcgactcct gtatcgcatg atgtaggcgc cacgaaaatg aatatccccg atatcgaact
      361 gctcgatcag aacggacgga agattcactt ttataccgat ctcgtcaaag gccagacagt
      421 cgttatcaat ttcatcttta ctacctgcac gacgatctgt ccgccgctcg gcgcaacttt
      481 cgcacgagtg caaaaagcgc tgggcgacaa agtaggacgc gacgtgcgct tcatttcgat
      541 cagcgtcgat ccggcgactg acactcccga aaggctgaaa gcgtggggcg cgaagtttca
      601 cgccggtgag ggctggacct ttgtcacggg gaataagccg caggttgacg aactgctgcg
      661 cgcgttgggt gcgtcgtccg cgcgtcgcga agatcattcg cctaccgtgc tcatcggcaa
      721 tgatgcgcat ggtacctgga ctcgtactta cggtttggcg aataccagca agttagtgga
      781 aatcatcaac gaagcgatcg caggtgctac taccaccgcg gagggttcta agtaatgaca
      841 agacaagttt tcatactatt cattttggca gttgttgtct ttgttacgac agacaactgc
      901 ttggcccagg cgtcggctgg ggggcaaacg ccgggccagg tttctacgcc gccagcgaaa
      961 cctgcggcgc cgccggcccc gcacgatcat tcgcacatga cgaagactgc ggaaaagtct
     1021 tcggttgctg cgaactactt tactgatgtc gaactgacta atcaggacgg caagaaagtc
     1081 cgtttctact cggacgtgct caaaggcaag actgtagtgg tcaatgcttt cttcaccact
     1141 tgcacttcgg tgtgtccacc gatgaaccgc aacatggaga agattcagga agttttcggc
     1201 gatcgcgttg gccgcgacgt ttttctcgtt tcgatcactg tcgatccgga agttgacaca
     1261 cctgcgcggc taaaagagta cgcgaagaag tttcacgccg gcccgggctg gatttttcta
     1321 accggtaaga aagagaacct cgactgggca ctctacaagc tgggccagta cgtcgagagg
     1381 aaagacgacc acaagaccat tttcatcatc ggcaacgaac caaccggatt gtggaagaaa
     1441 gcattcggca tggcgaacgt cgccgagctc gtgcaggtcg tcgaaagcgt cgttaacgat
     1501 aaaggcgaaa aaggcagcac caggtagcat gaaccggaaa tcaaagttct tcgtgggtat
     1561 gttgttggct ggcttcgtac tgacattgtc gggtgggctt ctggcgagtg tggcccaaca
     1621 aacgcccgcg ccgcaacagt cgagcaggag cctgacgccg caggagagac gcggaaaggc
     1681 gctctacatg cgcggtgaga gtgcttcagg aaaagagatc acggcgatac tcggcgattt
     1741 tgacgtgccg gcgtcaactc tgaactgcgc cggctgtcac ggtctgcgcg gtgaaggcaa
     1801 aaccgagggc ggcgtgactg cgggaaacct gacctggtcc aatatgatca agtcgtacgg
     1861 ccacacacat ccttcagggc gaaagcacgg agcgtttgac gaaaagctgt ttatccgcgc
     1921 aatggtccaa ggcgtagatc ccgcgggaaa cgaattagcc aaagcgatgc cgcgctacca
     1981 gatgtcgcct gaagaaatcg ccgatctcat cgcttatcta aaacgcatcg aagctgaccg
     2041 tgatcccggt ctaacagaaa cagctattaa ggtcggcacg atccttccca aagacggcgc
     2101 gctcgccgaa atcggcgcgg cgatgaagga tgtgctgact gcctacttcg caaacgtcaa
     2161 cgaaaagggc ggaatctaca accgccgcat tgagctgcaa acgatcgaca cgggcactga
     2221 cgcagcgacc accgcagcaa acgccaggaa gcatgtcgaa aacggcgagg tcttcgcgct
     2281 cgtcagcggc ctgagcgcgg gcgctgacaa agagcttgcg gcagtggtac gtgaagatga
     2341 aatacctttc atgggaccag tcacgctgtt gacacaaacc agtgggcagg acaatcgcaa
     2401 tctcttttac ctgttgcctg gcgcgggcca acaagcgcgc gcgcttgtaa acttcgcagc
     2461 caaaaagccc gagttaaaaa agtcgcagct cgcaatcgtc tactccgaaa acgaactagc
     2521 gctcgcggct gcggctgcaa ttgaggatca ggcccgtaaa cttggctgga cttccattac
     2581 caggagatct ctttcaaacg acgcggcggc aacagtcaca gcgctgaaag cagaaggcgc
     2641 tgaatccgtg ttctttcttg ccgccggggg cggcgaggcg gagttcatca acgccgctgt
     2701 gagcgcgaac tggactccgc acgtttttct tctcggcgca ctcaccggca g
//
LOCUS       DASTEY010000599         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1018634, whole genome shotgun sequence.
ACCESSION   DASTEY010000599 DASTEY010000000
VERSION     DASTEY010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018634"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFO72_10495"
     CDS             <1..292
                     /locus_tag="VFO72_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479765.1"
                     /translation="IARQQFNFQVSYFSGPWTAATQGRFVGRQFDDDLNTLPLRKFFT
                     LDAEASRSISEEVRLFVAVQNLTGTRYEISSTPVSTVGPPPLFRAGVRLSLR"
     gene            complement(428..1162)
                     /locus_tag="VFO72_10500"
     CDS             complement(428..1162)
                     /locus_tag="VFO72_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9479766.1"
                     /translation="MKIHIGTSGWHYKHWLGVFYPAGTRPAQMFQFYAQHFDTVEINN
                     SFYHLPSAKTFDNWRDSSPPRFLFAVKASRFITHMKKLKDPKPSSERFFLVADRLEKK
                     LGPILFQLPPRWKVNVDRFAEFLESLPRRHKYVFEFRDESWFVPEVYAVMRRHKAAFC
                     IHDFAEMNVPHEITANFSYIRFHGPTSAKYFGSYSSAQLRTWAERIEDWSRQLSSIYV
                     YFNNDPGGEAVKNGLELKRLIARRDL"
     gene            complement(1159..2241)
                     /locus_tag="VFO72_10505"
     CDS             complement(1159..2241)
                     /locus_tag="VFO72_10505"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET9479767.1"
                     /translation="MAETITLDDLRRFTVARNFFKPTNLQTALHQMGFVQADPIRAPA
                     RAQDLIIRHRVKDYRAGDLERHYTRLDIEEDFFVNYGYVSRWLYSLMHPRVDSPLSRT
                     DRKRARLLLEFVRERGRVHPREVDEYFAHGKVTNYWGGSSNATTHLMDMMHYRGMLRV
                     LRREKGIRIYAPREHARVPMSAAERRARTDALVDTVVRIYAPLPGSSLTYYVRRLQYA
                     APQWKRDLTDALKRAKKRLNHVRVGVDWYFPVNEDPSRSVVQEVVRLLAPFDPLVHDR
                     KRFELLWGWEYRFEAYTPVAKRKLGYYAMPLLWRERVIGWANVAVRNGAIECDIGYVG
                     SQPRERAFRRELEREVDRMRWFLGLK"
BASE COUNT          498 a          684 c          603 g          504 t
ORIGIN      
        1 gattgcccgc caacagttca atttccaggt cagttacttc agtggaccat ggactgccgc
       61 cacgcagggc cgtttcgtgg ggcggcagtt tgacgacgat ttgaatacct tgccgctgcg
      121 caagttcttt acgctcgatg cggaggcgtc gcgctcaatt tccgaagaag tacgactctt
      181 cgtcgcagtt caaaatctga ccggcacgcg atacgagatc agcagcacgc ctgtatcgac
      241 cgtaggcccg ccgccgctct ttcgggcggg tgttcgtttg tcgcttcgtt aatgatgcgc
      301 ctcggttgca caaagcacct ctgaccagaa gaatctttcc cgcaaaggcg caaaggcgca
      361 aagcgctacc gcgtctccaa ggggtttctt tgggccttgg cgcctttgcg cgagaaacat
      421 tttctcctca gaggtcccgc cgcgcgatca gtcgcttaag ctcaaggcca ttttttactg
      481 cctcaccgcc tgggtcgttg ttgaaataga cgtaaatcga cgatagctga cggctccagt
      541 cttcgatcct ttcagcccat gtgcgtagtt gcgcactcga atatgatcca aagtacttcg
      601 cgcttgtcgg gccgtgaaag cggatgtaac tgaagtttgc ggtgatctcg tgcggcacat
      661 tcatttcagc gaagtcgtga atgcagaagg ccgctttgtg acggcgcatc accgcataca
      721 cttccggtac gaaccagctc tcatcgcgaa actcaaagac gtacttgtgt ctgcgcggca
      781 gcgactccag gaactccgcg aaccggtcga cgtttacttt ccatcgcggt ggcagttgaa
      841 aaaggattgg accaagtttc ttttcgagtc gatcggcaac gagaaaaaac ctttccgaag
      901 acggcttcgg gtctttcagc ttcttcatgt gcgtgatgaa gcggctggct ttaactgcaa
      961 agagaaatct cggcggggac gagtcgcgcc agttgtcgaa cgtcttggcg gatggcaggt
     1021 gatagaaact gttattgatc tcgacggtgt cgaagtgctg ggcgtagaac tgaaacatct
     1081 gggccggccg tgtgcccgcc ggatagaaca cgccgagcca gtgtttgtaa tgccagcctg
     1141 atgtcccgat atggattttc atttcaaacc caggaaccag cgcatccgat ccacctccct
     1201 ttccaactct cgcctaaacg cacgctcgcg aggctgcgat cctacataac caatgtcaca
     1261 ttcaattgcg ccatttctca cagcaacgtt ggcccacccg ataacccgct cgcgccataa
     1321 caacggcatc gcgtaataac caagcttgcg cttcgccacg ggcgtgtacg cttcgaaccg
     1381 gtattcccaa ccccacaaca gttcaaaccg cttgcgatcg tggaccagcg gatcgaatgg
     1441 cgccagcaag cgcacaacct cctgaaccac agatctactc ggatcttcgt taaccggaaa
     1501 gtaccaatca acacccacgc gcacgtggtt caatctcttc ttcgcgcgtt taagcgcatc
     1561 cgtcagatcc cgtttccact gcggcgccgc atactgcaga cgccgcacgt aatacgtcag
     1621 actcgacccg ggcaacggcg cgtaaattcg caccacggtg tcaacaagcg cgtcagtccg
     1681 cgcacgccgc tccgcagcac tcatcggaac gcgcgcatgt tcgcgcggcg cataaattcg
     1741 aatccccttc tcgcgccgca acacccgcaa catcccgcgg taatgcatca tgtccatcag
     1801 gtgcgtcgtt gcattcgacg agccgcccca gtaattcgtc accttgccgt gcgcgaaata
     1861 ttcgtccact tcgcgcgggt gcacgcgacc acgctcgcgg acaaactcca gcagcagtcg
     1921 cgctcgcttt ctgtcagtcc gcgacagcgg cgagtccact cgcggatgca tcagcgaata
     1981 gagccagcgc gacacgtagc cgtagttgac gaagaagtct tcttcgatat cgagccttgt
     2041 gtaatggcgt tcgagatcgc cggctcgata atcctttaca cgatgacgaa taatgaggtc
     2101 ttgcgcgcgg gccggcgcgc ggatgggatc tgcttgcaca aatcccatct ggtgcagcgc
     2161 cgtctgtaga ttggtaggtt tgaagaaatt acgcgcgacg gtaaagcgtc gcagatcgtc
     2221 gagcgtgatc gtttcagcca cagatcagtt ttcaaactag ggacggttta cctgggaccg
     2281 caggcgtct
//
LOCUS       DASTEY010000600         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_343304, whole genome shotgun sequence.
ACCESSION   DASTEY010000600 DASTEY010000000
VERSION     DASTEY010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343304"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="thiD"
                     /locus_tag="VFO72_10510"
     CDS             <1..719
                     /gene="thiD"
                     /locus_tag="VFO72_10510"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET9479768.1"
                     /translation="IVADIKTIFALGCFPAAALTSITFQNTTGVFGAEHQSASTLRSQ
                     VEPIVRDVTVAAAKTGMLPTAEIVAEVARLFGEGNLPAPVVDPVVVATSGDVLIDEEA
                     FHILTSKLIPLARVVTPNIPEAEKLAGFTIESEADMRRAAERLQSLGARAVLVKGGHR
                     QMTAGEAVDVLLSESLTEFRSEYLDVGEVHGSGCTLSAAIAAGLGKGMTLEEAVGAAK
                     EYVTNAIRSAPRIGHGARPI"
     gene            complement(777..1259)
                     /locus_tag="VFO72_10515"
     CDS             complement(777..1259)
                     /locus_tag="VFO72_10515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9479769.1"
                     /translation="MANRTAVIEPNKGTIRVELLESDAPKTTENFITLAQRGYYDGII
                     FHRVIKGFMIQGGDPTGTGRGGESAWGGRFNDEIDSKSPVYQRGYKAGTMAMANAGPN
                     TNGSQFFIMHVDYPLPPSYTIFGRVTEGQDVVNAIATSQTGPGDRPVSEVKMEKVTIE
                     "
     gene            complement(1269..2150)
                     /locus_tag="VFO72_10520"
     CDS             complement(1269..2150)
                     /locus_tag="VFO72_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET9479770.1"
                     /translation="MAISLLALDLDGTLLDSRGRISERNRIAIDGAREKGVRVALVTG
                     RRFRDSRPVALELGLDIPLISHNGALTKHAGSLETVSVLPLPLAPAREALRVGRAAGA
                     DALLSDDHEGLGVLVYDHIRGENSAAHKYITWARRIHGEEGANAVQQVTSLENYLDHD
                     PIHLAFSGGCAEMDQLEETLKSELGSSVKLLSTKYIEQDFTLLDIVNPAASKGAGVAA
                     AAAELGATREEIMAIGDNYNDLEMLLFAGVGVVMANAPLTLREIAGLHPTASNREDGV
                     ALAIEQFILNRPQLINQ"
BASE COUNT          486 a          636 c          642 g          508 t
ORIGIN      
        1 gcattgttgc ggatatcaag accatcttcg cgttggggtg ttttccggcg gcagcgctga
       61 cgtcgatcac gtttcagaac acgaccggag tctttggcgc tgaacatcaa tccgcttcga
      121 cgttgcgatc gcaggtggaa ccgatcgttc gcgatgtgac cgttgccgcg gcgaagacgg
      181 gaatgcttcc gactgcggag atcgttgctg aagtggcgcg actctttggt gaagggaatc
      241 tgccggcgcc ggtcgtcgat ccggtggtgg ttgcgacgtc cggcgacgtg ttaattgacg
      301 aagaagcttt ccacattctg acgagcaaat taatcccact tgctcgcgtc gttacgccca
      361 acattcccga agcggaaaag cttgcgggat tcacgatcga aagcgaagca gacatgcggc
      421 gcgcagccga gagacttcag tcgctcggtg cacgcgcagt gctggttaaa ggcgggcaca
      481 ggcagatgac ggctggtgaa gcagttgatg ttctgttgag cgagagtttg acggagttta
      541 gaagcgagta ccttgacgtc ggtgaagtgc acggctctgg atgcactttg tcagccgcta
      601 ttgcagcagg ccttggaaaa gggatgactc tcgaagaagc agtcggagcg gcgaaggaat
      661 acgtaaccaa cgcaatccga tcagcgcccc gcatcggcca cggagcaaga ccaatttgag
      721 ccgcagatga cgcggataaa agtccaaatt ttatctgcgt catccgcggc tcagttttac
      781 tcgatcgtca ctttctccat cttaacttcg gagaccggcc gatcacccgg gccggtctgg
      841 ctcgtcgcga ttgcattcac aacgtcctgc ccctcagtaa ctctgccaaa gatcgtgtaa
      901 cttggcggta acggataatc gacgtgcatg atgaaaaact gcgagccatt cgtgtttggt
      961 ccagcattcg ccatcgccat cgtgcccgct ttataacctc tctgatacac aggcgatttg
     1021 gaatcgatct catcgttgaa gcgtccgccc cacgccgact cgccgccacg cccggtgccg
     1081 gtcggatcgc caccctggat catgaagcct ttgatcacgc ggtgaaagat gatgccgtcg
     1141 tagtagccac gttgtgcgag ggtgatgaag ttttccgttg tcttgggtgc gtccgactcc
     1201 aaaagctcaa ctcgaatcgt tcccttgttc ggttcgatta ctgcggttcg atttgccatg
     1261 ttttctcctt actggttgat taactgcggc cggttcaaga tgaattgctc gatcgcgagc
     1321 gccacaccgt cctcgcggtt cgaagcagtc gggtgcaagc cggcaatttc acgcaaagtc
     1381 agcggcgcat tcgccatcac gacgcctacg cccgcgaaga gcaacatttc gagatcgttg
     1441 tagttgtcgc cgatggccat gatctcttcg cgagtcgcgc ccaactcggc tgctgctgcg
     1501 gcgacgcctg cgccttttga cgctgcggga ttaacgatgt ccagcaacgt gaaatcctgt
     1561 tcgatatatt ttgtgctgag aagtttcaca ctggatccga gctccgactt gagcgtctct
     1621 tcgagctgat ccatttccgc gcagccgccg gagaatgcga gatggatcgg gtcgtggtcc
     1681 aggtagtttt ccagcgacgt aacttgctgt accgcgtttg cgccttcttc gccatgaatt
     1741 cttcgcgccc aggtaatgta tttatgagcg gcggagtttt caccacgaat gtgatcgtaa
     1801 acaagcacgc ccagtccctc gtgatcgtca ctcagcaacg cgtccgcgcc ggccgcacgc
     1861 ccgactcgca atgcttcacg cgcgggtgcg agtggcagcg gcagcaccga aactgtctcg
     1921 agtgacccgg cgtgtttggt cagcgcgcca ttgtgtgaga tcagcggaat gtcgaggccg
     1981 agctccaaag ccaccggacg cgaatcacga aagcgccgcc cggtcacgag cgccacgcgc
     2041 acgccctttt cgcgtgcacc atcaattgcg atgcgattgc gctcagaaat acgtccacga
     2101 gaatcgagca gagtcccgtc cagatcgaga gccagaagac ttatcgccat agagaaagca
     2161 caaggtaaag gaagcgaagg cagaaggcaa tccgagggaa atatgtccta taagacttat
     2221 aggacttata tgacctatga cctattgcct tccccctgct ttcacatcgt ga
//
LOCUS       DASTEY010000601         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1463787, whole genome shotgun sequence.
ACCESSION   DASTEY010000601 DASTEY010000000
VERSION     DASTEY010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463787"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="VFO72_10525"
     CDS             complement(<1..1675)
                     /locus_tag="VFO72_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET9479771.1"
                     /translation="MAIEITGVPDARGVEWLARFARRLIGFSVVITAVAVILSADALI
                     RSYKFYSQIVDARLASGYLTSRPGLYAAPRVLQVGQKLSRDKLINALRRAGYLETTAS
                     NVWSGSFNADGNAIEIRPGRSSRKQPAVVRVEYADSEIVELTGDGVTLESFTLEPEVL
                     SNDLSAKAGKREALSYDEIPPVLVQAILAIEDRRFFDHSGVDVSGLARALLRNAGDER
                     MGQGGSTITQQLVKNTYLSPEKTLQRKYAEAMLSLALERRLSKKDIFTLYCNEIYLGQ
                     RGAVSVRGMKEAAAVFFGKQLRDLTLAEAATLAGMIQSPAHYSPLRHPEAARARRNVV
                     LAAMRENSAITREQNDAASAEPTAITPPVDFNNSLAPYFVDYVNRVADSGEGRRVYTT
                     IDLELQDLAEQALRKQLVDRYKDQSAIPQAALVALDPHTGNVLAMVGGRDYAESQLNR
                     ATDARRQPGSTFKPFVYAAAVEDGMSPVRLFVDAPREFIYDRNRTYRPANYGGGYSMG
                     EVTMRTGLVKSLNVVTVDVALQTGLARIANLAAAFGLPKPERYPALALGT"
     gene            complement(1730..2602)
                     /locus_tag="VFO72_10530"
     CDS             complement(1730..2602)
                     /locus_tag="VFO72_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9479772.1"
                     /translation="MRPSIALALSGGAARGMAHVGVLRALVENKIPIDYIAGTSAGSL
                     VGGAFASGMPLDQIEDLGRKMRWRDVGRVTMSRLGVQSNERLEQFLRENLPVTRFEDL
                     QLPFAAVATELKSGAAVIMRDQGDVPFAIRASCAIPGWYVPVTDEQGRQLVDGGLVAV
                     VPASVARSFGADIVIAVDVNAEGAKFLGPTSSVIGVVLQSMLVVQKTASHYQLSLADF
                     VIRPKVGHIRWDEMGRAEELMTAGYNAGTECVPEILSLINAAIKAQPKWYQLRRRKKL
                     PAKTTSPFPEFLAH"
     gene            complement(2727..3383)
                     /locus_tag="VFO72_10535"
     CDS             complement(2727..3383)
                     /locus_tag="VFO72_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479773.1"
                     /translation="MKQFAKTLVLLLAFSSMSAAQQVVDKMVATVNAGVRTELITYSD
                     LMWQLALQPNTQLDNPNSEQLNRALRLLIDQRLILQEAEKLPTITPTPKEISDARADL
                     ARNFSSEPVFRERLQRVGLTSEKLDEIIEERLKMEKYLDFRFRNFVVISQKEIADYYR
                     DVYVPRFKTRTPGRIVPTLEEARAEIEKTLMEAKIESDTDAFLDTLRQRAEIVTLSPV
                     "
     gene            complement(3391..>4119)
                     /locus_tag="VFO72_10540"
     CDS             complement(3391..>4119)
                     /locus_tag="VFO72_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9479774.1"
                     /translation="LRQLGGTISFNRFQATYRRYYKMSALRDTVFAGNFTLGLANLFN
                     PVDRDGNGSIDEIDRTLPISERFFSGGSTTLRGFNFEEAGPREVVIPEGQFRDRERNL
                     VFLNPFTVPVGGNALAIVNLEARIPLTRSIQVVPFYDGGNVFRRVGDLFGKNDPAPVP
                     PGDILAAIRAVNLRAHWTNTVGLGFRWQTPFGGALAVDYGFLLKPPEFLIPQRGPDGI
                     SFDGTPAVFRLHRGNLHFRITQTF"
BASE COUNT          929 a         1233 c         1080 g          877 t
ORIGIN      
        1 cagtgcccaa cgcaagcgcc ggatatcttt ccggtttcgg cagaccaaac gccgctgcaa
       61 gattggcgat ccgggccaat ccggtttgca aagcaacgtc gacagtgacg acgttcaacg
      121 acttcaccaa accggtgcgc atcgtaactt cacccatcga ataaccgccg ccgtagttgg
      181 ctggacgata agtgcggttg cggtcgtaaa taaattctcg cggcgcatcc acaaacaacc
      241 gcaccggcga cattccatct tcgacagcgg cggcatagac gaacggcttg aatgtcgaac
      301 cgggttggcg gcgtgcatct gtcgcgcgat tcaactgtga ctctgcgtaa tcccgcccgc
      361 ccaccatcgc caacacatta cccgtgtgtg gatccaacgc gaccagcgct gcctgcggta
      421 ttgcactctg atctttgtat cgatccacca actgtttcct cagcgcttgt tcggccagat
      481 cctgcaactc gagatcgatg gtggtgtaaa cacgtcgccc ttcacctgaa tcagcgacgc
      541 ggttgacgta gtcgacaaag tacggcgcga gtgaattgtt gaagtcgaca ggaggtgtaa
      601 tcgcagttgg ctccgcagac gcggcatcat tttgttcgcg cgtgatcgcg ctattctccc
      661 gcatcgcggc cagaacgaca tttcttcgcg cgcgcgcggc ttcaggatga cgcaaaggcg
      721 aatagtgcgc gggactctgg atcattcccg caagcgtcgc cgcttccgct aacgtgaggt
      781 cgcgcagttg tttgccgaag aaaacggccg cggcctcttt catgccacgc acgctaaccg
      841 cgccgcgttg gcccagatag atctcgttgc agtaaagcgt gaagatgtct ttctttgata
      901 gccgccgttc gagcgccagc gacaacatgg cttcggcata tttgcgctgg agcgtctttt
      961 cgggtgaaag gtaagtgttt ttgacgagtt gctgagtgat ggttgaaccg ccctggccca
     1021 ttcgttcgtc accggcattt cgcaacaggg cacgcgcgag cccgctgaca tcaacacccg
     1081 aatggtcgaa gaatcttcgg tcctcaattg ccaggatcgc ctgcacaagc accggcggaa
     1141 tctcgtcgta gctcagcgcc tcgcgcttgc ctgctttcgc agaaagatcg ttggacagca
     1201 cttccggttc gagtgtgaac gattcaagtg tgacaccgtc accggtcagc tcgacgattt
     1261 cactatccgc atattcaact cgaacaacag ccggctgttt tcgactgctg cggccgggtc
     1321 gaatctcgat cgcgtttcca tcagcgttga aactcccgct ccaaacattg ctggcggtag
     1381 tttccagata acctgcgcga cgcagcgcat tgatcaactt gtcgcgcgac aacttctgac
     1441 caacctgcaa cacgcgtggc gcagcgtaca aacccggtct gctcgtgagg taaccgctcg
     1501 ccagccgcgc gtcgacgatc tgggagtaga acttgtaaga acggattagg gcatccgcgc
     1561 tcaagatgac cgcgaccgca gttatgacca cgctgaagcc aatgagcctg cgcgcgaaac
     1621 gcgccagcca ctcgactccg cgcgcgtcgg gcacacctgt aatctcgatt gccacagata
     1681 acctcgcgac ccgtcagtgc ttcttaattc gtatgggatt tgtaaacggt taatgcgcga
     1741 ggaattctgg aaaaggtgag gtggtcttgg ccggaagttt tttacggcgc cgaagttgat
     1801 accactttgg ttgcgccttt attgcggcgt ttatcagcga cagaatctcg ggaacacatt
     1861 cagtaccggc gttgtagccg gcagtcatca gttcttcggc gcgtcccatc tcgtcccagc
     1921 gaatgtgccc gactttcggt ctgataacga agtcagccaa cgacagttgg tagtgcgagg
     1981 ccgtcttctg gaccaccaac atcgattgca gcacaacccc aatcaccgaa ctcgttggtc
     2041 cgagaaactt ggcgccttca gcattcacgt ccactgcgat cactatatcc gcgccaaacg
     2101 aacgagccac cgaagcagga acaaccgcga ccagcccacc atcgaccagt tgtcgtccct
     2161 gttcatccgt caccggtacg taccaacccg gaatcgcaca actcgcgcga atagcaaacg
     2221 gaacgtcgcc ctggtcgcgc ataatcacgg ccgcgcccga cttcagctca gtcgccacag
     2281 ccgcaaacgg cagctgaaga tcttcaaacc ttgtaaccgg taaattttcg cgaagaaatt
     2341 gttccaaacg ctcgttgctc tgcacgccaa gccgcgacat cgtgacgcgg cccacatcgc
     2401 gccagcgcat cttgcgtccg agatcttcga tctggtccaa cggcataccc gaagcgaacg
     2461 cgccgccgac aagcgatccg gctgacgtcc cggcgatgta atcaattggt attttgtttt
     2521 cgacgagcgc gcgtagcacg cccacgtgcg ccataccgcg cgcggcgccg ccggaaagtg
     2581 ctaaagcgat tgaagggcgc atgtttctat agagctccag accggccttg cccgaaaatt
     2641 cctatttctc tgtgccttgg tgtctcggtg gttttttttt gagggaaatc gttaccacaa
     2701 aggcacagag acacaaagaa cgaagctcaa actggactta acgttacaat ttccgctctc
     2761 tggcgaagcg tatccaaaaa cgcatccgtg tcggactcga tctttgcttc catcaaagtt
     2821 ttttcaatct ccgcgcgcgc ctcttcgagc gtgggaacga tacggccggg agtgcgcgtc
     2881 ttgaagcgcg gcacataaac gtcgcgataa taatcggcga tctctttttg actgatcacc
     2941 acaaaattac gaaaacggaa atcgagatac ttttccatct tcagccgctc ttcaatgatc
     3001 tcgtccagtt tttcgctggt caacccgacg cgttgcaacc gctcgcgaaa cacaggctcc
     3061 gaagaaaaat tgcgcgcgag atcagctcgc gcgtcgctga tctccttcgg cgtaggcgtt
     3121 atcgtcggca acttctccgc ttcctgtagt atcaaccgct gatcgatcag caaacgcagc
     3181 gcgcgattca actgctcaga gttcggattg tccagttgcg tgtttggttg cagcgccaat
     3241 tgccacatca agtcggaata agtgatcaac tccgtcctca cccctgcgtt aaccgtcgcc
     3301 accatcttgt cgacgacctg ctgcgccgcg gacattgaag aaaacgccag caacagaact
     3361 aaagtttttg caaattgctt cataagccgc tcaaaacgtt tgggtaattc ggaaatgcaa
     3421 attaccgcgg tgcaaccgaa acactgccgg cgtgccgtcg aacgaaatgc cgtctggtcc
     3481 acgctgcgga atcaaaaact ccggcggctt caaaagaaac ccataatcga ccgccagtgc
     3541 gccaccaaac ggcgtctgcc agcgaaatcc cagacccacg gtgtttgtcc agtgcgcgcg
     3601 gaggttgacg gccctgatcg ccgcgagaat atcacccggc ggaacgggcg ccggatcgtt
     3661 tttgccaaac agatcgccga ctcggcgaaa cacgttgccg ccatcgtaga acggcaccac
     3721 ctgtatcgac ctcgtcagtg ggatgcgagc ttcaaggttc acgattgcca gcgcgtttcc
     3781 gcccaccggc accgtgaaag gattgagaaa aacaagattt ctttcccggt ctcgaaattg
     3841 accttccgga atcacgacct cgcgtggacc agcctcttcg aaattaaagc cgcgcagagt
     3901 cgtcgaaccg ccggagaaga atcgctcgct gatgggtagc gtgcgatcga tctcgtcgat
     3961 gctgccgttg ccgtcgcggt cgaccgggtt gaacaggttc gcgagaccaa gggtgaaatt
     4021 gccggcgaaa acggtgtcgc gcagcgcgct cattttgtaa tagcgacgat acgtcgcctg
     4081 aaagcgattg aaagagatgg ttccgccaag ctgccgtaa
//
LOCUS       DASTEY010000602         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1381582, whole genome shotgun sequence.
ACCESSION   DASTEY010000602 DASTEY010000000
VERSION     DASTEY010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381582"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFO72_10545"
     CDS             complement(<1..625)
                     /locus_tag="VFO72_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9479775.1"
                     /translation="MAAVEKRGPDAATLELWRVAETGEVEALARVLKRVPDINARNEH
                     GVTALMRAAQFGHVRMVRALLEHGADANMTRNDKFTALALAAFFGHTQVVRVLMEHGA
                     DSQASTRYGTSPHMWATARTFDEVVNQLEKPAPPEKVALPRRNDSVGPVAPSLGPRPA
                     PAVGAASAGPVVGAVIRTLKDPPEIWDLVHEVPKGFNARSAFKSRLME"
     gene            complement(630..1892)
                     /locus_tag="VFO72_10550"
     CDS             complement(630..1892)
                     /locus_tag="VFO72_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479776.1"
                     /translation="MYKSLIISVVIVLSTLSVGPVSAQTRLHKDFASSGETHSESKRL
                     YNEGVKYALAGLFAQAAQLFQQAIKLDPGFADAHFSLGHAYYDMGRWQQAIDSFQRAV
                     ELNPKDLEAQDRLEQAREMLARETGKKPTATPKTSPLTSTRAPQPVPEKVSLSDKPAV
                     PPAETSANEANAAANDIALTKLYRVGPGDVLDVRVSDSASTQSTLFTVTPAGLLEHPS
                     LAEPLHVAGMSVDEITTTIETDLKRRALSENPKVSVGVRDYASHTILVSGLVKDSGTK
                     ILRREAIPLYVVVADAQPLPEAAKVTLVRSESNQTYEIDLAQPAEMNLLVRPGDVITL
                     HAAVTQFLYIGGEVKSPGEKTYRRGLTLTQAILAAGGATNKAKEARLGRDDGRGFLTV
                     TTLKLKDIESGKIPDPMVRPGDRIMIVR"
     gene            complement(2315..>3313)
                     /locus_tag="VFO72_10555"
     CDS             complement(2315..>3313)
                     /locus_tag="VFO72_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9479777.1"
                     /translation="GCVEYWRELQRVDLTGYNTIESADDLDDLRKALGARKISLWSIS
                     YGTHLALTTIRRHPQSIHRAILAGTEGPDHTYKLPSNIQKHLEDLAALIKEDPELGKE
                     IPDFLGLIKSVFDRLDAQPQTVEITDPRTKQNVKVVVNKFVLQYIVANNIGTTVTASF
                     PALFYRASKGDFTNPAQVWLNTSRQGIGSAMSYMMDCASGQTTARRERISREAKGTLL
                     EDISNFPLPDICDEWKAPDLGDQFRSPVTANVPVLFISGTLDARTPVSNAEEYRTGLA
                     NSTHIIIDGAVHSDPLFLSSPKIKDGMLEFLKGQPVTATRISAPPMKFAPLLPPKP"
BASE COUNT          662 a          941 c          928 g          782 t
ORIGIN      
        1 cttccatcaa gcgggacttg aatgcggagc gcgcgttaaa ccccttcggc acctcatgga
       61 ccagatccca gatttccggc ggatccttca gagttctaat cactgcacct accactggac
      121 cagcggacgc ggctccaacc gcgggcgccg gccttggccc aaggctcggt gccaccggac
      181 caactgaatc gtttcgtctc ggcagcgcaa ctttctccgg tggtgcgggt ttctcgagct
      241 gattgacgac ttcatcaaac gtacgggctg tcgcccacat gtgcggcgac gtcccataac
      301 gagttgaagc ctgagaatcc gcaccgtgtt ccatcaaaac acggacaact tgcgtatggc
      361 cgaagaacgc cgccaacgcg agagccgtga acttgtcgtt acgagtcatg ttcgcatccg
      421 cgccgtgctc gagtagcgcg cgaaccatcc tgacgtgtcc aaactgcgcc gcacgcatca
      481 acgccgtcac gccgtgttcg ttgcgcgcgt tgatatcggg cacacgcttt aacacgcgcg
      541 cgagcgcttc aacttcgcca gtttccgcta cgcgccacaa ctccagcgtc gcagcgtctg
      601 gtcctctttt ctcaacagca gccatgttct cagcgcacaa tcatgatccg gtctcccgga
      661 cgaaccatcg ggtcgggaat ttttccggat tcgatgtctt tcagcttaag agtagtaaca
      721 gtcaaaaacc cgcggccgtc gtcgcgcccc agccgtgctt ccttcgcttt gttcgttgcg
      781 cctcccgcag cgaggatcgc ttgcgtgagc gtcagtccac gtcgatacgt cttttcacca
      841 ggcgacttca cttcaccgcc gatgtacaaa aactgcgtca cagccgcgtg tagcgtgatc
      901 acgtctcctg gacgaaccag cagattcatc tccgccggct gggcaagatc gatttcgtaa
      961 gtctggttcg actcgctgcg aaccaatgtc actttggcgg cttcgggcag cggctgcgcg
     1021 tcggcgacga ctacgtaaag tgggatggcc tcacggcgca ggattttcgt gcctgagtct
     1081 ttcaccagac cacttacgag aattgtgtga ctcgcataat cgcgaacgcc cactgaaacc
     1141 tttggattct ccgacaaagc gcgacgtttg agatcggttt cgatcgttgt cgttatttcg
     1201 tcgacactca tgcctgcaac atgcaaaggc tcggcgagcg atggatgctc caacagcccg
     1261 gctggcgtaa ctgtaaacaa ggtagactgc gttgatgcgg aatcgcttac ccgcacgtcc
     1321 agcacatctc caggcccaac gcgatacagt ttcgtcagag cgatatcgtt agcggcggcg
     1381 tttgcttcgt ttgctgaagt ctctgcgggc ggcacggcag gcttgtcact taatgaaact
     1441 ttttcaggaa ccggctgcgg cgcgcgcgtt gatgttagcg gtgatgtttt cggcgttgcg
     1501 gttggcttct ttcctgtctc gcgcgccagc atctcgcgag cctgttcgag cctgtcctgc
     1561 gcttccagat ctttcgggtt caattcgacg gcgcgttgaa agctgtcgat cgcctgctgc
     1621 cagcgcccca tgtcgtagta ggcgtgaccg agtgagaaat gggcgtcggc aaaacctgga
     1681 tccagtttta tcgcctgctg gaagagttgc gcggcctgcg caaaaaggcc ggcgagcgcg
     1741 tactttactc cctcgttata caagcgcttt gattccgaat gagtctctcc ggaagacgcg
     1801 aagtccttgt gtagtcgggt ctgagccgaa accgggccaa cagacaaagt cgacaagaca
     1861 atgacgacgg aaatgattaa cgatttgtac atgagaagcc ccttgaaaca ggacgtactg
     1921 ctacacgaac ggggttcttg aaaaacgggg gactacttgt cagggtcagg gggtggcttg
     1981 cttataccac ttttcgcccc gcatttccaa cttttgccaa catctgcttg tcagctgtgc
     2041 gcacgtttgc ttgctgatcg gctgtcccct caagagtctt ctttcattct caccgggctt
     2101 tcagcccggt gttaccgcca cgcggttttg agaaaccgtt ttaacggttt cgctggcgaa
     2161 tccaccgaaa accgttgaaa cggtttcctc cgtattcact gctcctgcac cgggctaaaa
     2221 gcccggtgag aatgagagag gactctttga ggcaaagctt tgctaatccg ttgtgaaacc
     2281 tttgcttgtt taccgggggt gccagcttgt ctggttaagg tttgggcggc agcagtgggg
     2341 cgaacttcat gggcggggcc gatattctcg tcgccgtcac tggctgcccc ttgagaaact
     2401 ccaacatgcc gtctttgatt tttggcgagg aaagaaacag cggatcgctg tgcacggcgc
     2461 cgtcgataat gatgtgcgtg ctgtttgcaa ggccagttcg gtactcctct gcattactga
     2521 ccggagtgcg ggcgtccagc gttccgctga taaagagcac cgggacgttt gcagtcactg
     2581 gtgaacgaaa ctgatcgccg aggtcaggtg ctttccactc gtcacaaatg tcgggaaggg
     2641 gaaagttaga aatgtcttcc agcagagtgc ctttcgcctc gcgcgaaatg cgttcgcgtc
     2701 gcgccgtggt ctggccggag gcgcaatcca tcatgtaaga catcgctgag ccgatgccct
     2761 ggcgcgaagt gtttagccat acctgggccg ggtttgtgaa gtcgcctttc gatgcgcgat
     2821 agaacaaagc gggaaagctt gccgtcacgg tcgtcccgat gttattcgca acgatgtact
     2881 gcaagacaaa cttgttcacg accactttga cgttctgttt tgtgcgtgga tcagtgatct
     2941 caaccgtttg cggttgcgca tcgaggcgat caaagaccga cttaatcagg ccgagaaaat
     3001 caggaatctc ttttcccagc tccggatcct ccttgatcaa tgcggcgaga tcctcgagat
     3061 gcttctggat attgctcgga agtttgtagg tgtggtcggg accttccgtg ccggcgagga
     3121 tcgcgcgatg aatgctctgc gggtgccggc gaatcgttgt cagcgcgagg tgagttccgt
     3181 aactgatgga ccagagactg atctttctcg cgccgagcgc cttacgcaga tcgtcgaggt
     3241 cgtcagcact ttcgatagtg ttgtagccag tcagatcgac gcgctgtagc tcgcgccagt
     3301 actcgacgca acc
//
LOCUS       DASTEY010000603         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1381585, whole genome shotgun sequence.
ACCESSION   DASTEY010000603 DASTEY010000000
VERSION     DASTEY010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381585"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFO72_10560"
     CDS             complement(<1..923)
                     /locus_tag="VFO72_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479778.1"
                     /translation="MPLLMAASAEVITKPVASAISLRWIISARDDVIWFIGSVVSSYV
                     LLFLYVWGIVPLVPMVAAWAILIDAPHVFGTFSRTYFDRAERKNRARLLWGSLLFFAI
                     GPLMVLAGAGLVFLFVAALWAYYHLVKQHYGFMVLYKKKNGDLARVDNVLDRLLLLFA
                     FNYPFVEFIARDPEAMRRVPELLRGGANGLAKLLLAGTILIFVVWLGRQIQRAFTGEP
                     LNVPKYLLLAAAIPMHWIVLLTPMTHKPIAIVAILTIYHNFQYHRLIWFHNQKYANAR
                     EKYGAAELISRRVLFYVAFGIIFGIIYQGPR"
     gene            938..1549
                     /locus_tag="VFO72_10565"
     CDS             938..1549
                     /locus_tag="VFO72_10565"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HET9479779.1"
                     /translation="MNKQLFQAHPWHGVTPGTPDLVNAYIEIVPTDAVKYELDKTSGH
                     LRVDRPQRFSSMSPTLYGFIPQTYCGREVGELCALRTGRRGIEGDKDPMDICVLSEKT
                     FAYGGFFLHARPIGGLRMIDGNQADDKIIAVLESDLAFGEVVEIGDVAKGLVDRLKHY
                     FLSYKQLPSDKEKKVEIVDVYDRAEAREVITRSLRDYESEFSR"
     gene            complement(1811..>2273)
                     /locus_tag="VFO72_10570"
     CDS             complement(1811..>2273)
                     /locus_tag="VFO72_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9479780.1"
                     /translation="GRMIGINSQILSPSGSSAGVGFAVPVSIAKRVVPQLLQNGAVRR
                     PKLGITTRNVEAFRNQVDLPTADGVLIIQVARGSGAAQAGLRGMQQTEMGDVELGDII
                     VGINNDKVGNSDDLFRVLDKHQIGETVQVQIYRDGRRMSVPVRLMESATPR"
BASE COUNT          545 a          662 c          614 g          452 t
ORIGIN      
        1 tgccgcggtc cctggtaaat gatcccaaag ataatcccga acgccacata gaagagtacg
       61 cgccggctaa ttagttcagc agcgccatac ttctcccgcg cattcgcgta cttctgattg
      121 tgaaaccaga tcaaccggtg atactgaaag ttgtgataga tcgtcagtat cgcaacaatc
      181 gcgattggct tgtgcgtcat cggcgtcagc aacacgatcc agtgcatcgg tatcgccgct
      241 gccagcaaaa gatacttcgg aacatttaac ggctcgcccg taaacgcacg ttgtatctgc
      301 cggcccaacc acactacgaa aatcagaatc gtgcctgcca gcagcagctt ggccaagccg
      361 tttgcaccac cgcgcaacag ctccggcacg cgtctcatcg cttccggatc gcgcgcgata
      421 aactcaacga acggatagtt gaacgcaaac agcagcaaca aacgatcgag cacgttatcg
      481 acacgcgcaa ggtcgccgtt cttcttttta tagagcacca tgaaaccgta gtgctgtttg
      541 acgaggtgat aataggccca caacgctgcc acgaagagaa acacgagccc cgctccagcc
      601 aataccatca gcggtccaat cgcgaagaag agcagcgagc cccaaagcag ccgcgcgcgg
      661 ttctttctct cagcacgatc gaagtaggtg cgcgagaacg tgccgaagac gtgtggcgca
      721 tcgatcagaa tcgcccaggc cgcaaccatc ggcaccaaag gcacgattcc ccacacgtat
      781 agaaatagca gcacgtagct ggagacgacc gagccgataa accagatcac gtcgtcgcgc
      841 gcgctgatga tccaacgcag cgagatcgcg cttgcaaccg gcttggtgat gacttcggcg
      901 ctggcggcca tgagtaaagg cattttatca tcggccgatg aacaaacaac tctttcaggc
      961 acatccctgg catggtgtca cgccgggaac gcccgatctc gtaaacgcct acatcgaaat
     1021 cgttccgact gacgccgtca aatacgagct cgataaaact tcaggacatc tacgggtcga
     1081 tcgcccgcag cgcttctcgt ccatgtcgcc gacgctttac gggttcattc cgcagacgta
     1141 ttgcgggcgc gaggttggcg agttgtgcgc attgcgcacc ggccgccgcg ggatcgaagg
     1201 cgacaaggat ccgatggata tttgcgtgtt gtcggagaag acgtttgcgt acggcggatt
     1261 cttcctgcac gcgcgaccga ttggcgggtt acgcatgatc gacggcaatc aggctgacga
     1321 caagatcatc gcggtgctgg aatcggatct cgcgtttggc gaggtcgtcg aaatcggtga
     1381 cgtggcgaag ggattagttg atcgtttgaa acactacttt ctcagctaca agcaactacc
     1441 gagcgacaaa gagaagaagg tcgagattgt tgacgtttac gatcgcgccg aagcgcgcga
     1501 ggtgatcacg cgaagcttga gggattacga aagtgaattt agccgctgat aacgcggata
     1561 aaatccggat tttctatctg cgtttatctg cggattcaga aatgccgcag cgggggcaaa
     1621 cgagcccccg ccactgatcc tggtccaccc atttagccag tgccggacct tgcgaccggt
     1681 gctggccctg atggtggtga tgatgatgtt tgcgctcagc gtcctctgcg ctgcagtgtt
     1741 tacagagcca gccatacccc agattgaaga agtgggtcgt ctggtgcata ggacctgagt
     1801 ttacatcaga tcaccgcggc gtagcagatt ccatcaaacg caccggcaca gacatgcgcc
     1861 tgccgtcacg gtagatctga acctgaaccg tctcgccaat ctgatgttta tcgagcactc
     1921 ggaacagatc gtctgaattt ccgactttgt cgttattgat gccgacgata atgtcgccca
     1981 actcgacgtc gcccatctcc gtctgttgca tgccgcgaag accagcctgc gctgcgccac
     2041 tgccgcgcgc cacctgaata atcaacacac cgtcagcagt aggaagatcg acctgatttc
     2101 gaaacgcctc aacgttccgt gtcgtgatgc cgagcttggg ccggcgcacg gcaccgtttt
     2161 gcagcaactg cggcacaacg cgcttcgcga tactcaccgg caccgcaaaa ccaacgcccg
     2221 ccgaactgcc ggacggcgaa agtatctgcg agttgatgcc gatcatgcgg cca
//
LOCUS       DASTEY010000604         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1134322, whole genome shotgun sequence.
ACCESSION   DASTEY010000604 DASTEY010000000
VERSION     DASTEY010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134322"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="xth"
                     /locus_tag="VFO72_10575"
     CDS             complement(<1..601)
                     /gene="xth"
                     /locus_tag="VFO72_10575"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET9479781.1"
                     /translation="MLIATWNVNSILARMPSVVRWLDDVKPDVLCMQETKCTDDKVPV
                     IAFQERGYHCQLFGQQSYNGVAILTRGACETTQRGFPNDDGEAQSRLITTMFNGISIV
                     NVYIPNGQMVGSEKFEFKLAWMRRLREFFDSHYDKSTPVLLCGDFNVAPEERDVHDPR
                     LWQGRIMFSEKELAAVQDIKAWVFTDAFRLHTEAGGKFTW"
     gene            complement(622..1800)
                     /locus_tag="VFO72_10580"
     CDS             complement(622..1800)
                     /locus_tag="VFO72_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9479782.1"
                     /translation="MTGEQYSSSLAAQIFERFRKRQSDIESFIRTLVEIESPSGDVEG
                     SREVVAALSAAAQSVNGVDAIEPFEVPGFGQHLVIKAFQEQTEAGQILIVGHTDTVHS
                     RGSLAQRPWRRDTDRAYGPVVFDMKANCALVIEVLRTFAELGVKPRYAVNVVLTCDEE
                     VGSASGWPLIERVAKAANTRCAFVLEPPAPGGRVKTGRKGTGIYAIKVEGKAAHAGLE
                     PEKGASAILELARQTERLHALNLSGSGITLNVGVVHGGTRSNVVAAQAEGEIDVRFAT
                     EAESREVDRILSTLKPVDERTKVFVSGGINRPPLERTPAVVELFEKARSISANLGFEL
                     GEAQVGGASDGNFIAALGIPVLDGLGISGDGAHAVHEHIEVDDIPRRGALITGLLLSV
                     "
     gene            complement(1797..>2652)
                     /gene="menC"
                     /locus_tag="VFO72_10585"
     CDS             complement(1797..>2652)
                     /gene="menC"
                     /locus_tag="VFO72_10585"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET9479783.1"
                     /translation="VFALMKPVRGHRMAKATIETACWDLEAKKLGVPLWKHLGGTRRE
                     IASGVSIGIQDSPDILLEKIRKEVDAGYQRIKIKIKPGWDTAIVARVRKEFPETPLMG
                     DANSAYTLADVPLFKQLDRFNLMMLEQPLSHDDIFDHATLQQQIQTPICLDESIRTAA
                     DATHAIAMASCKIINVKLGRVGGHTHAKRVEQVARENDIPVWSGGMLESGIGRAHNIA
                     MSTLAGFSLPGDVSASARYWEEDIIDPPVMVSAKGTITAPDAPGIGFNLNLPRIDALT
                     VRKQTINL"
BASE COUNT          585 a          806 c          669 g          592 t
ORIGIN      
        1 accacgtaaa cttcccacca gcttcggtat gcagacgaaa cgcgtctgtg aatacccacg
       61 ccttgatgtc ctgcacagcc gcgagctctt tttcgctgaa catgatgcgt ccctgccaaa
      121 gccgtgggtc atgtacgtcg cgctcttcag gcgccacgtt gaagtcacca cacaacaaaa
      181 cgggcgttga tttgtcgtag tgagagtcga agaactcccg caaacgtcgc atccacgcca
      241 gcttgaattc aaatttctct gaaccgacca tctgaccgtt tggaatgtaa acgttaacaa
      301 tgctgatgcc gttgaacatg gtggtaatca gacgtgattg cgcttcgcca tcgtcgttcg
      361 gaaagccacg ctgagtagtc tcgcaggccc cacgtgtgag aattgccacg ccgttgtacg
      421 actgctggcc gaaaagctgg cagtggtaac ctcgctcctg aaacgcgatg accggaacct
      481 tgtcgtccgt gcatttcgtc tcctgcatgc agagcacatc gggcttgacg tcgtcgagcc
      541 agcgcacgac gctcggcatg cgcgcgagaa ttgagttgac gttccaggtt gcaatcagca
      601 taagccgcag atcaacacag actacacgga caggagtaaa ccagttatta gggcgcctcg
      661 gcgggggata tcatccacct cgatatgttc gtgaacggcg tgcgcgccgt cgccgctgat
      721 tccgagtcca tccagcaccg ggatccccag cgccgcaatg aagtttccat cagacgcgcc
      781 accgacctgc gcttccccaa gttcaaaacc aagattcgcg gaaatacttc gcgccttctc
      841 aaacagttca acgactgcgg gcgtcctttc gagtggcggt cgattgattc cgcccgagac
      901 gaaaactttg gttcgctcat cgaccggctt gagagtcgat aggatacggt caacctcgcg
      961 cgactcggct tcggtcgcaa agcgcacgtc gatctcgcct tcggcctgcg ccgcaacgac
     1021 gttcgaacga gtgccaccat gaacgacgcc gacgttcaat gtgattccag aacctgaaag
     1081 attcaacgca tgaagacgtt cggtctgtcg cgccaattcc agaatggcgc tcgcgccttt
     1141 ctcaggttcc agtcctgcgt gcgccgcttt cccttcgacc ttgatcgcgt aaatgccagt
     1201 tccctttcgt ccagtcttaa cgcgtcctcc aggcgcaggc ggttccaaca cgaatgcgca
     1261 acgcgtgttc gcagctttcg caacccgctc tattaacggc cagccggacg cactgccaac
     1321 ttcttcatcg caggtcagca cgacgttcac ggcatatcgc ggctttacac ccaactcggc
     1381 gaacgtgcgc aacacttcaa tcaccagcgc acaattcgct ttcatatcaa acacgactgg
     1441 cccatatgcc ctgtctgtat cccttcgcca cggacgctgc gcaagcgagc ctcgcgagtg
     1501 cactgtgtct gtgtgcccaa caatcaagat ctgtcccgcc tcggtttgct cttgaaacgc
     1561 tttgataacc agatgttgac cgaagccggg gacctcgaac ggttcaatcg catcaacgcc
     1621 gttaacgctc tgcgctgccg ccgatagagc agcaacaact tcgcggctgc cctcgacgtc
     1681 gcccgacggc gactctattt ccaccaatgt gcgtatgaac gattcaatgt cgctctgcct
     1741 cttgcgaaac ctctcgaaaa tctgagccgc aagagaggat gaatattgtt cgcctgtcat
     1801 agattgatag tttgcttgcg caccgtcaga gcgtcgatgc gcggcaggtt aagattgaac
     1861 ccaatacctg gcgcgtccgg cgccgtgatc gttccttttg cactcaccat taccggcgga
     1921 tctatgatgt cttcttccca gtaacgcgca ctcgccgaca cgtcgccagg cagactgaaa
     1981 ccagcaagtg tggacatcgc aatgttgtga gcgcgcccaa tgcccgactc caacatgccg
     2041 ccagaccaaa ccggaatgtc gttctcacgc gccacttgct cgacacgttt tgcgtgcgtg
     2101 tgtccgccca cgcgcccgag tttcacgttg atgatcttgc acgacgccat cgcaatcgcg
     2161 tgagtcgcat ctgcggcagt gcggatcgac tcgtccagac agattggcgt ctgaatctgt
     2221 tgttgcaatg tcgcgtgatc gaagatgtcg tcatgcgaca acggctgctc gagcatcatc
     2281 aagttgaatc gatcgagttg tttgaaaagt ggaacatcgg caagtgtgta agccgaatta
     2341 gcatcgccca tcaaaggagt ttcgggaaac tctttgcgaa cgcgcgcgac aatagcggtg
     2401 tcccaaccgg gtttgatctt gattttgatc cgctggtaac ccgcatcgac ttccttacga
     2461 atcttttcga gaagaatatc gggtgagtct tgaataccga ttgaaacacc cgaggcgatt
     2521 tcgcgacgcg taccgccaag atgtttccac aaaggcacgc cgagtttctt cgcttcgagg
     2581 tcccagcaag cagtttcaat cgtcgctttc gccatccggt gacctcgcac aggtttcatc
     2641 aacgcaaaca ct
//
LOCUS       DASTEY010000605         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_574055, whole genome shotgun sequence.
ACCESSION   DASTEY010000605 DASTEY010000000
VERSION     DASTEY010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574055"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3572
                     /locus_tag="VFO72_10590"
     CDS             <1..3572
                     /locus_tag="VFO72_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479784.1"
                     /translation="TDVLNLEDVISSHVAEAILPHLTVDQRLRLAKRGTDNPQAHEAY
                     LRARFYWNTFTEEGFARAIVCYQQAIALDPKYALAYAGVAAYHNWLGVYSVMPFAECA
                     AAAYEAASTAVELDPNLAEGHTALAQAVLFRDFAWASSEHHLVRAIELDPNYAPARIW
                     YSLQLAMEGRFSEALRQANIGRDLDPLAVISRFALAWCSYHGRRYEEAHRFISSTLEN
                     EPRNLMMRYVLSFVLSRLSRHDEAIEAAEKCVEMLGKASHTLGRLGAAQAAAGNLDEA
                     QKVLDEMDQLSTRRYVSPYHLALVTCALGRKEAALDLLEQAYDTGDAKVMWMGVDPEL
                     DSLHGHPRFNDLLQKLDHRLASLPPIAGQLREEQESIAVLPFRVLSPPGENTGDEYLG
                     IGLTDALITRLSNVRRLVVRPTSSVLRYHGANVDPLVAGRDLGIDYIVDGSLRRVGDR
                     IRVTAQLLTVPEGVTRWAEHFDEDSTDVLQIEDSISEKVASALLPQLTGDEQHRLSKR
                     GTDSSEAFEAYLRGRYYWNSYTETGLAKALDCYQRAIKLDPDYALAYTGIADYYNWLG
                     VFGVKPFAECSAAAKQAASKAVELDSTSAEAYSALGFATACHDFDWSVAEGHHRRALE
                     INPNYATAHNWYAFHLVMEGRFDEGIEQMLRTRELDPLSPAVLQALGWCYYHARRFDE
                     SLATFRNMLEAIPDFPYGLVTYSWVLRHTGAVDEAVKAGEKALDLSGGGQFYTAQVGS
                     AYAAAGRQQDARAVLERLTQMMVHGYVSPYHRAMIHLHLGERDRALDLLQQAYSIKDA
                     WVVWLGVEPQWDPLRGEPVFEKILRDLRHPAAGRKAARVSAAAAAEPKRRRKDHIPPI
                     TQIISSSPETQAGENEEARQLYTAGRYYATRRTAEGLRQAIERLERAVELDPNFAIAH
                     SELADCYALLNWYVEPPPPKAWEHAKASALRAVEADPDLAEAHASLGFVRLHYDRDWI
                     DAERELRKAIQLKPSNQVAHRWYAYSLSAMGRHDEAYAEIERARQISPQSAVIATAVA
                     NVLFLAGKFDESIVQCRKSLELDPGAVAAHTVLRWAYELKGMHSEALAAFEQERSFAG
                     DTPTTHAKRAHVLAAIGKRDEAQTILEEIIAKRNQNWVTAYEIAIIYTLLDDFDSAFR
                     WLAQAEREHAVGFTFVRVDPHLTKLRSDPRFNDFLRETEKTIP"
     gene            3569..>4394
                     /locus_tag="VFO72_10595"
     CDS             3569..>4394
                     /locus_tag="VFO72_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479785.1"
                     /translation="MTAIENLDAWVREVVQWHFDPETGCQFWLDYAKDLSWDPRKEIR
                     TYQDLSRFGFFQDEWLRGGPVRRWVPKAFASRPIYTFETGGSTGVPKSRINIDDFRID
                     YEAFSDTLPDEFFPPGSDWLSLGPTGPRRLRLAVEHLAQHRGGICFMVDLDPRWVIKL
                     LKMGQPKMAEQYKQHVVEQGLTLLRAHDNIRCLFTTPKLLEALCEKVSLRKAGITGVF
                     CGGTEMTPQFHRFAVEELLEGAYFAPTYGNTLMGLAVHKPRIPEDNYAIIYYPPVPR"
BASE COUNT          923 a         1336 c         1259 g          876 t
ORIGIN      
        1 ttactgacgt tctgaatctc gaagacgtga tctcctcgca tgtcgccgaa gccatcctgc
       61 cgcacttgac cgtcgatcaa cggctgcgtc tcgccaagcg aggaacagac aacccgcagg
      121 cgcacgaagc ttacctgcgc gcccgttttt actggaacac gtttacggaa gaaggtttcg
      181 cccgcgcgat tgtctgttat caacaagcga tcgcactcga tcccaagtac gcgctcgctt
      241 acgcgggtgt ggctgcgtat cacaactggc tcggcgtcta cagtgtgatg ccgtttgccg
      301 agtgtgccgc agcggcgtac gaggcagcgt caaccgcagt cgaactcgat ccgaatctgg
      361 ccgagggcca caccgcgttg gcgcaggccg tactcttccg cgacttcgcc tgggccagtt
      421 ccgaacatca tctcgtgcgg gccatcgaac tcgatcccaa ctatgcgccg gcgcgcatct
      481 ggtactcgtt gcaactcgcg atggagggac gtttcagcga ggcgttgcga caggcaaaca
      541 tcggccgcga cctggatcca ctcgcggtga tttctcgttt cgcactggcc tggtgctcct
      601 atcacgggcg ccgctacgag gaagcgcatc gctttattag ctcgacgctg gaaaacgagc
      661 cgcgcaatct gatgatgcgc tacgtattga gtttcgtgct cagccgtctc agccgtcacg
      721 acgaagcgat cgaagccgcc gagaaatgtg tcgagatgct cggtaaagcg tcgcacacac
      781 tcggcaggct cggcgcagct caggccgccg ccggcaatct cgatgaagca caaaaagtgc
      841 tcgacgagat ggaccagctt tctacgcgcc gctacgtctc tccatatcac ctggcgcttg
      901 tgacttgtgc gctcggccgc aaagaagcgg ctttggattt actcgaacag gcttacgata
      961 ccggcgacgc aaaagtcatg tggatgggcg tcgatccgga actcgattct ttgcacggtc
     1021 atccgcgttt caacgatctg ttacaaaaac tggaccaccg tctcgcgtcc cttccgccaa
     1081 tcgctggaca actacgcgaa gagcaggagt cgatcgccgt acttcccttc cgcgttctca
     1141 gcccgccggg agagaacacg ggcgacgagt atctcggcat cggactgact gacgccctga
     1201 tcacgcgcct cagcaatgtg cggcgtctcg tggtgcgacc gacaagcagt gtcttgcgtt
     1261 atcacggggc gaacgtcgat ccactcgtcg cggggcgcga tctgggtatt gactacatcg
     1321 tcgatggcag tctccgccgt gtcggcgatc gcattcgcgt gacggcccag cttctcacgg
     1381 tgcccgaagg tgttacgcgt tgggccgaac acttcgacga ggactcgacg gacgtgttgc
     1441 agatcgagga ttcgatttca gagaaagtcg ccagtgcgtt gttgccgcaa ttgaccggcg
     1501 acgagcaaca tcgactgtcc aagcgcggca ccgacagctc cgaagccttc gaagcatatc
     1561 tgcgcgggcg ttactactgg aactcttaca ccgaaacagg tctcgccaaa gcactcgact
     1621 gctaccaacg cgcgatcaaa ctcgatccgg attacgcgct cgcctatacg ggtatcgccg
     1681 attactacaa ctggctcggc gtctttggtg tcaagccttt cgctgaatgc tccgccgcgg
     1741 ccaagcaagc cgcaagcaag gcagtcgaac tggattcaac gtcggcagag gcctacagcg
     1801 cactcggctt tgcgacggct tgtcacgact tcgattggag cgtcgccgaa ggccatcacc
     1861 ggcgcgctct cgagatcaat ccgaactacg ccacggcgca caactggtat gcattccatc
     1921 tggtaatgga gggacgtttc gacgaaggca tcgagcagat gctgcgcacc cgcgaactcg
     1981 acccgctctc accggccgtg cttcaggcgc taggctggtg ttactaccac gcgcggcgtt
     2041 ttgatgaatc gctcgccacg ttccggaaca tgctcgaagc aatccccgac ttcccctatg
     2101 gtcttgtcac ctactcctgg gtgctgcgtc acacaggcgc cgtcgacgaa gcggtgaaag
     2161 cgggtgagaa ggcgttggat ctttccggcg gcggacaatt ttatacggcg caagtgggtt
     2221 ccgcttacgc ggctgcgggc agacaacagg acgcgcgcgc agtactggaa cggctgaccc
     2281 agatgatggt ccatggttac gtttcgcctt accaccgcgc gatgattcac ctgcatctcg
     2341 gcgaacgcga ccgcgctctc gatctgcttc agcaggctta ctcgatcaag gacgcgtggg
     2401 tggtctggtt gggtgtcgaa ccgcagtggg atccactgcg tggtgaaccg gtattcgaga
     2461 aaatactgcg cgacctgcga catcccgcgg caggccgcaa agcggcacgt gtgtcagcgg
     2521 cagccgccgc tgaaccgaaa cggcgccgca aagatcatat tccgccgata acgcagatca
     2581 tcagttcctc gccggaaact caagccggtg aaaatgaaga ggcgcgccag ctctacaccg
     2641 ccggccgtta ttatgctacg cgccgaacag ccgaaggttt gcgacaagcg atcgaacgac
     2701 tggaacgcgc cgttgaactg gatccgaact tcgcgatcgc gcattccgaa ctcgcggact
     2761 gttacgcttt gctcaactgg tacgtcgaac cgccgccgcc caaagcatgg gaacacgcga
     2821 aggcgtcggc attgcgcgcc gtcgaagcag atccggatct tgccgaggcg catgcgtcgc
     2881 tcgggtttgt aaggctgcat tacgatcgcg actggattga cgccgaacgc gaactgcgca
     2941 aagcaattca attgaaaccg agcaaccagg tggcccatcg ttggtatgct tacagccttt
     3001 ctgccatggg ccggcacgat gaggcttacg ccgagatcga acgcgcgcgt caaatctcgc
     3061 cgcagtcggc ggtcatcgcg acggccgtgg cgaatgttct gttcctcgcg ggcaagtttg
     3121 acgagtcgat cgtgcagtgc cgcaagtcct tggaactcga tccgggcgcg gtcgcggcgc
     3181 acaccgtttt acgttgggct tacgaactaa aaggtatgca ttcggaagcg ctcgctgcgt
     3241 ttgaacagga acgcagtttt gcgggcgaca cgcccaccac tcacgccaag cgcgcacacg
     3301 tgctcgccgc gattggcaag cgggacgagg cgcaaacgat cctcgaagag attatcgcga
     3361 aacgaaacca gaactgggtc acggcttacg aaatcgccat catctacact ctgctcgacg
     3421 acttcgacag cgcgtttcgc tggttggccc aggctgaacg cgaacatgcc gtcggcttta
     3481 ctttcgttcg cgtcgatccg cacctgacaa aactgcgcag cgatccgcgc tttaatgatt
     3541 ttctacgcga aacggagaag acgattcctt gacggcaata gagaacctcg acgcctgggt
     3601 acgcgaagtc gttcaatggc atttcgaccc ggaaaccggc tgtcagttct ggctggacta
     3661 cgccaaagat ctgtcctggg atccgcgaaa agagattcgc acctaccagg atctttcacg
     3721 gttcggtttt ttccaggacg aatggttgcg cggtggacca gtgcgacgct gggtgccgaa
     3781 agcttttgcc agccggccca tctacacgtt cgagacaggc ggcagcacgg gcgttccgaa
     3841 gtcgcgcatc aacattgacg actttcgcat cgattacgaa gccttcagcg atacactgcc
     3901 tgacgaattc ttcccaccgg gttctgactg gttatcgctt ggaccaaccg gcccgcgacg
     3961 tttgcgtctt gcggtcgagc acctggccca acatcgcggc ggcatctgtt tcatggtcga
     4021 cctcgatccg cgctgggtca tcaaactgct gaagatgggc caaccaaaaa tggccgaaca
     4081 gtacaagcag cacgtagtcg aacagggtct cactctgctg cgagcgcacg acaacattcg
     4141 ttgcctgttc accacgccca aattgttgga ggcgctctgc gaaaaagtct cgctcaggaa
     4201 ggccggcatc acaggtgtct tttgcggcgg gaccgagatg acgccgcaat ttcaccgctt
     4261 tgcagtcgaa gaactactcg aaggcgccta tttcgcgccg acttacggaa acacgctgat
     4321 gggtctcgcc gtacataaac cgaggattcc ggaagacaac tacgcgatca tctattaccc
     4381 gcccgtaccg cgag
//
LOCUS       DASTEY010000606         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_788395, whole genome shotgun sequence.
ACCESSION   DASTEY010000606 DASTEY010000000
VERSION     DASTEY010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788395"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFO72_10600"
     CDS             complement(<1..1136)
                     /locus_tag="VFO72_10600"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9479786.1"
                     /translation="MRLSPTEIYRDRKIFLIGSTGFLGKVTLSMLLYRFPNIGKIYLT
                     VRARSQEESETRFWNNIITAPPFDPLRERYGSALEGFVNDKVIVRGGDIAETNFGYSE
                     EEAQAIAEDVDVIINSAGNVTFNPTLESGLRTNVVGTQNVIAFARRMKRPALVHVSTC
                     FVAGNRSGAVWESDPVIGYFPRKNESEMKGVQFSVDQEIADCAKLADRVRDEARDAMM
                     TARFRELARKRLNEEGRDVDDPDALGLAVARERKVWTRTRLTDLGVERAAYWGWPNIY
                     TYTKSLGEQLVAAETGMVRSIVRPSIVESAMSYPFPGWNEGFTTTAPIIFLIRKGQTQ
                     IPADPKLILDITPVDQVASVMLAVSAQACVEEPRLVFQAATGDA"
     gene            complement(1172..>2045)
                     /locus_tag="VFO72_10605"
     CDS             complement(1172..>2045)
                     /locus_tag="VFO72_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479787.1"
                     /translation="KVVQLGKIVDEQLNVFHIETAINNRMYGADMDFLLRNEDDFSAF
                     DRMKFEAMRFTMSKLPRPARRKLLHSRPAQYEMTACYAGKTELVHEKIVAKGFEQYAI
                     PIRGQCDILITGIPDISPYNVYSILNPLLVQVMALGYHFNFYRNKPLLKKGGVLILHH
                     PCYDDFDHQFHPSYIEFFHRLLPETRDAFTLREKYEREFANNPSYIEMYRRGNAYHGA
                     HPFFMWYWGENGRQHVGRVIAAGAENAHVPGILGWERADNLTEAIAMARSYMGNSAEI
                     TMLHQPMIGIADME"
BASE COUNT          432 a          582 c          572 g          459 t
ORIGIN      
        1 ttggcatcgc cagttgctgc ctgaaaaacc agtcgcggct cttcgacaca cgcctgggcc
       61 gaaaccgcga gcatcaccga cgcgacctgg tctaccggcg tgatatcaag aatcagtttt
      121 gggtctgcgg gaatctgtgt ctggcctttg cggatcagga agatgatcgg cgcggtggtc
      181 gtgaagccct cattccagcc cggaaacggg tacgacattg cagactcgac gatactcggt
      241 cgaacgatgc tgcgcaccat tccggtttcg gcggcgacga gctgctcgcc gagtgatttc
      301 gtgtaggtgt aaatgttggg ccaaccccag taggcggcac gctctacgcc gagatctgtc
      361 aggcgcgtgc gcgtccagac ttttcgctcg cgagcgacag cgagcccgag tgcgtcggga
      421 tcgtcaacat cacgtccttc ttcgttgagt cgcttgcgtg cgagctcgcg gaaacgcgcg
      481 gtcatcatcg cgtcgcgggc ttcgtcgcgc acgcgatccg ccagcttggc gcagtcggca
      541 atctcctggt ccaccgagaa ctgcacgccc ttcatttcgc tttcgttttt gcgtgggaaa
      601 taaccgatta ccggatcgct ttcccaaacg gcgccagacc gattgccggc gacgaagcaa
      661 gtagaaacgt ggaccagcgc gggacgcttc atccggcgcg cgaaggcgat cacgttctgc
      721 gtgccgacga cgttagtgcg caggccactc tcgagtgtgg gattgaaagt tacgttgccg
      781 gcggagttga ttatgacgtc gacgtcttcg gcgatggctt gcgcttcttc ttcgctgtaa
      841 ccgaagttag tttcagcgat gtcaccgcct ctgacgatca ctttgtcgtt gacgaagcct
      901 tcaagcgcgc tgccatagcg ttctcgcagt gggtcgaacg gcggagcggt aatgatgtta
      961 ttccaaaaac gagtttccga ctcctcctgc gaacgagcac gtacagtgag atagatcttg
     1021 ccgatgttcg ggaaacgata gagcaacatg ctcaacgtca ccttcccgag aaatcccgta
     1081 ctcccaatca gaaagatctt cctgtctcga taaatttcag tcggagagag tctcataaaa
     1141 aatccgtaaa aaatccgcgt atatctgcgt tctattccat gtcagcaatg ccgatcatcg
     1201 gctgatgcag catcgtgatc tccgcggagt tgcccatgta actgcgcgcc atcgcgatgg
     1261 cctcagtcag attgtccgct cgttcccatc ccaggattcc cggcacgtgc gcattctcag
     1321 cgcccgccgc gatcacgcgg cccacgtgct gccgtccatt ctcaccccag taccacatga
     1381 aaaacgggtg cgcgccgtga taggcattgc cgcgccggta catctcgatg tagctcggat
     1441 tgtttgcaaa ctcgcgctcg tacttctcac gcaaggtgaa cgcatcgcgc gtctccggca
     1501 acaggcgatg gaaaaactcg atgtagctcg gatgaaactg gtgatcgaaa tcgtcgtaac
     1561 acggatggtg caggatcagc acgccgcctt ttttcagcaa cggcttgttg cgatagaagt
     1621 tgaagtgata accgagcgcc atcacctgga ccagcagagg gttcagtatc gagtaaacgt
     1681 tgtacggcga gatatctgga atgccagtga tcagaatgtc acactggccg cgaatcggaa
     1741 tcgcatactg ctcgaaacct ttcgcaacga tcttctcatg cacgagctca gtcttcccgg
     1801 cataacacgc ggtcatctcg tactgcgccg gacgcgagtg caagagcttg cggcgtgcgg
     1861 gccgcggcag cttgctcatt gtgaagcgca tggcttcaaa cttcatgcga tcaaaggcgc
     1921 tgaagtcgtc ttcgttgcgc agcagaaaat ccatgtcggc gccgtacatg cgattgttga
     1981 tagcagtttc gatgtgaaag acgttgagtt gctcgtcgac gatttttccg agctgtacga
     2041 cctta
//
LOCUS       DASTEY010000607         7605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_805200, whole genome shotgun sequence.
ACCESSION   DASTEY010000607 DASTEY010000000
VERSION     DASTEY010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805200"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFO72_10610"
     CDS             complement(<1..180)
                     /locus_tag="VFO72_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479788.1"
                     /translation="MRKLICFGVAVTLVFGLAPFLSAQDQQYDLLLKNGRIVDGSGNA
                     WYRGDLAIRGDTIVRI"
     gene            complement(217..906)
                     /locus_tag="VFO72_10615"
     CDS             complement(217..906)
                     /locus_tag="VFO72_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET9479789.1"
                     /translation="MCSLHQAARTAAVLLLFLSATAFAQDATGYADLPRFQHVSERLY
                     RGAQPRRGGFSRLRELGIDTIINLRGPNKQTRAEEAEARALGLNYFNIELPRWGRPQD
                     ARVARILEIIAAPENGEVLIHCRDGVDRTGMIVAIYRMTHDKWNSHDALAEAERYGMR
                     RIQFWMRDYAEDYGERVVKLGPETALQSPGVDEDFGDRIGAGMRIVEKGALRARKYAG
                     RFVRKFSGSLP"
     gene            complement(949..1878)
                     /locus_tag="VFO72_10620"
     CDS             complement(949..1878)
                     /locus_tag="VFO72_10620"
                     /inference="COORDINATES: protein motif:HMM:NF023695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HET9479790.1"
                     /translation="MSSRFIAIAVTLLLALAIGNLLHRAPHAASPAPMTAFSGGTFEA
                     SGVAHVPGTDAVLFVDDGRTKEIFLMQLGEDRKQSGPIKRIDISTNIIDLEGITFDGE
                     YFYVVGSQSKSKGADLSGLARFKFDAASQRAIDTQAASGLKKFLADNVAELRGMENTK
                     YSDGGINVEGVAWDPQNKRLLVGLRSPVVEGHALVVPLKPRDPKTALSFDNLEVEGRK
                     AIRLPLGGAGIRSIEYDESRKAFYLITGAGPNAEKMDFKIWEWSGDGATPALRQMETF
                     DRRLKPEGITRVATGDRDFIFIVFDTSGYAATD"
     gene            2503..5199
                     /locus_tag="VFO72_10625"
     CDS             2503..5199
                     /locus_tag="VFO72_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET9479791.1"
                     /translation="MSTENTDAKSSTRWHTLTIEETFARFESGKRGLSSGEASRRLEQ
                     FGPNELQVKRRVSPWAILLEQFKNVLIIILLLATALSAFLGHGVEAIAITVIVLFAVI
                     LGFIQEFRAERAIEALREMAAPSATVIRDGRDQRVPARDLVPGDLIVLATGDKVPADA
                     RLTEAVNLQTVEAPLTGESAPVEKHSRPLEEETLSPGDQRNMAFAGTAVTYGRGRALV
                     VATGMATEFGKIARMLEGVDTAKTPLQKNLDRIGKALARAAIAVVLIIVVLGLFRGQP
                     FIEMLIFGVALAVAVVPEALPAVVTISLALGVKRMVRRNALIRRLPAVETLGSTSVIC
                     SDKTGTLTKDEMTVRRLYVAGKMIEVSGRGYEPNGKFSTNGSGVGTSEPLSMLLQAAA
                     LASDARVEQRNGSGTWEVKGDPTEGALVVVAAKAGLDKPELDEKFSRISEIPFTSETK
                     RMTTLHQTPDGVVAYTKGAPEVIVQSCASQLTEAGVEPLDEARRAEVLEYARQMAGEA
                     LRVLAVAYKPQATVEDAEKEMTLLGLAGMIDPPRREAKAAVRKCEEAGIKVIMITGDH
                     PLTAKAVAEELGLSKNGRVVNGAELEAMDDSQLEREVESIEVFARVSPAHKLRVVTAL
                     QNRGQVVAMTGDGVNDAPALKKADIGIAMGITGTDVSKEAAAMTLTDDNFASIVAAVE
                     EGRGIFSNIKKYLMYLLSSNIGEIGLMAGASLAGLPLPLTAVQILYVNLATDGLPALA
                     LAVDPPEHGLMQRPPRDPRTGIFTRPVVLLMVVGGVWSAIVNLGLFVWALNSGRPVAE
                     AMTMTFVSLVLIQFFKAYNFRSDRQSVLREPFANKWLNLAIVWELILLALILYVPLLE
                     RIFGTIALPVNDWLIIVAAAFTVSPVLELTKWFERKWFAELR"
     gene            5822..6205
                     /locus_tag="VFO72_10630"
     CDS             5822..6205
                     /locus_tag="VFO72_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance family protein"
                     /protein_id="HET9479792.1"
                     /translation="MSDFGEVVPILNVKDLSASIDYFVQQLGFEKKWDWGDPPNFGCV
                     GRGKVEIFLCQGCQGQAGMWMSIFVDQVDRLYEEYKENGAKILDPPTNYPWGTREMLV
                     EDLDGHRFRMSGESTEPSACEGGTD"
     gene            complement(6202..6981)
                     /locus_tag="VFO72_10635"
     CDS             complement(6202..6981)
                     /locus_tag="VFO72_10635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET9479793.1"
                     /translation="MRNFVDRRSIVRTIWGDPDLILLIFAGSAAEFALNRAVDWLFFT
                     GKIPGDPIGRLFSTVRYAQDIVFVDEETAQRTLNRINAAHHAVERQRVQTIPDWAFRD
                     VLYMLVDYSERAYSLLHRPLSPSEQNELYAVFRRIGNGLSIPELPETYAEWKLDRRRH
                     MQRDLTFSRHSSLLFQQYRRHLGFWRYYLLLRVQALLVPVEVRRLLQLSSNKLMLNLV
                     KTYGVISTMNLRSVVHTLLVPPQYWPELRQFDRNAVIETGR"
     gene            complement(6990..>7605)
                     /locus_tag="VFO72_10640"
     CDS             complement(6990..>7605)
                     /locus_tag="VFO72_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET9479794.1"
                     /translation="DQATSGPVAEGNSGGGTGMVVHQFKGGTGTSSRRFQIDAATYTV
                     GVLVQANYGSRSGLTIAGVPVGREITDLMPQRVPADTGAIIVVVATDAPLLPHQLKRV
                     VKRAALGIARNGGIGGNSSGDIFIAFSTANAGAGKETSLANVKMLPNDHIDPVFLATV
                     QATEEAIINAMVAAETMTGVNGNTVYAIPHDRLREVMKKYNRLR"
BASE COUNT         1669 a         2030 c         2209 g         1697 t
ORIGIN      
        1 gatgcggacg atcgtgtcgc cgcggatcgc aaggtctccg cggtaccagg cattgccgct
       61 accgtcgaca attcttccgt ttttaagtag cagatcgtat tgctgatctt gagcagacag
      121 aaacggagcc aggccgaaaa ctaaagtcac ggcaacgccg aaacagatca attttcgcat
      181 ccgcgatcct cttgagacag gaaaggcaag ttgagtttat ggcagcgaac cggagaactt
      241 gcggacaaag cggcccgcgt attttcgcgc cctgagggct cctttttcga cgatgcgcat
      301 accggcgccg atacgatcgc cgaagtcttc atcaacacca ggagactgaa gtgctgtctc
      361 gggtcccagc ttcacgacgc gttctccgta atcttcggcg tagtcgcgca tccaaaactg
      421 aattcgtcgc atgccgtagc gttcggcctc tgcgagcgcg tcgtggctgt tccatttgtc
      481 atgcgtcata cgatagatgg cgacgatcat gccggttcga tcgactccgt ctcggcagtg
      541 gattaaaact tccccgttct ccggcgcagc tatgatctcg agaatccgcg ccacccgcgc
      601 gtcttgaggc cgtccccacc tgggaagctc aatgttgaag taattcagtc ccagagcgcg
      661 cgcttccgcc tcttccgctc tggtttgttt gttcgggccg cggagattga tgatggtgtc
      721 gatgcctagt tcgcgaagtc gactgaagcc gccgcggcgt ggttgagcgc cgcgatacag
      781 tcgttcactc acgtgctgaa atcgcggcaa gtcagcgtag ccggtcgcgt cctgagcaaa
      841 cgcagtcgcc gaaagaaaca gcaacaggac cgcggctgtt cgagccgcct ggtggaggct
      901 acacatctct tcctctacgg ttagttgtaa gcgaatgact aacgattgtt agtcggttgc
      961 ggcatagccg ctggtgtcga acacaataaa gataaaatct cgatcgccag ttgcaacgcg
     1021 agtaatacct tcaggcttga gacggcgatc aaacgtttcc atctggcgca gcgccggcgt
     1081 tgcgccgtcc ccgctccact cccagatttt gaagtccatt ttctcagcgt tcggtcccgc
     1141 acccgtaatc aggtagaacg ctttgcgcga ctcatcatac tcgatgcttc tgatcccagc
     1201 gccacccaac ggcaaccgga ttgcttttcg tccttcaacc tcaaggttgt cgaaagacag
     1261 tgcagtttta ggatcgcgag gtttcagcgg cacaaccagc gcgtggcctt cgactactgg
     1321 agaacgtaag cctaccagca atcgcttgtt ttgtggatcc cacgcgacgc cttcgacgtt
     1381 gatgccgccg tcgctgtact tcgtgttttc catgccgcgg agttcggcga cgttatccgc
     1441 cagaaacttt ttgagtccgg aagcagcttg agtatctatg gcacgttggc ttgccgcgtc
     1501 gaacttaaag cgtgcaagac cggagagatc ggcgcctttc gacttcgact gcgatcccac
     1561 aacgtagaaa tactcaccat cgaaagtgat accttcgaga tcgatgatgt tcgtcgaaat
     1621 atcgattcgc ttgatcggac ctgactgttt gcgatcttcg ccaagttgca tgaggaagat
     1681 ctccttcgtc cgcccatcgt caacaaagag tactgcgtca gtgccgggca catgcgccac
     1741 gccggaggct tcgaacgtgc cacccgagaa cgcagtcatc ggtgcaggag aagccgcatg
     1801 tggagcgcga tggagcaggt tgccgatggc taaggccaga agaagagtta ccgcaatagc
     1861 tatgaagcga ctgctcattt cggtcgaacc tcacccggta aacgaaccgt aaaataatcg
     1921 cagattgtgt cgagagagtc actgtgtcaa gttgctttgc tcaaaagtcc ctctctcatt
     1981 ctcaccgggc tttaagcccg gtgccagagc agtgaagacg gaggaagccg tttcaacggt
     2041 ttttgtggat tcgccagcga aaccgttaaa aacggtttct cacaaccgcc tgccgttaca
     2101 ccgggctgaa agcccggtga gaatgaaaga gtttacaagt ggctgaactc gacggttacg
     2161 tcgaagttac accgggctga aagcccggtg agaatgaaag aggactttga ggcaaagcaa
     2221 gcgtcaagtc agatttcacc ggcaaaaaca aatgagtccg gtttttcgca accagtggct
     2281 tagaccggcg atcagttgta gaatcgcagc acgaaaaggg gagtaggtca cctgccactg
     2341 tggtccggtg cgaccgtcaa cacggtgcct gacgtaggca cccggcgcat cgggtagggc
     2401 aaacaatgag gctccacgac caagaccttt tgcggcttag gcggcgcgcg aaaggttttt
     2461 cctttaggcg cggcgtctat atgtccacga cgcgacgcgc ttatgtctac agaaaatacc
     2521 gacgctaaat ccagcacgcg ctggcataca ttaaccatag aagaaacgtt tgcacggttt
     2581 gagtccggca agcgcggtct tagctccggc gaagcctcga ggcgattgga gcagtttggt
     2641 ccaaacgaac ttcaagtcaa acgccgcgtc tcgccctggg ccatcctcct cgaacaattc
     2701 aaaaacgttc tgatcatcat tctgctgctt gcaacagcgc tctccgcgtt tttgggccac
     2761 ggcgtcgagg cgatcgcaat taccgtcatc gtccttttcg ccgtcattct cggcttcatc
     2821 caggagtttc gcgccgagcg cgctattgaa gctcttcgcg aaatggccgc accttcggcg
     2881 acagtgattc gcgatggacg cgatcagcgc gttccagcgc gcgaccttgt gcccggagat
     2941 cttattgttc tggccacggg ggacaaggtc cctgccgacg ctcgattgac cgaagcagtc
     3001 aatttgcaaa cggtcgaggc gccactgacc ggcgaatccg ctccggttga gaagcattcc
     3061 cgtccactcg aagaagagac gctgtctccc ggcgatcaac gaaacatggc cttcgccggc
     3121 accgccgtta cttacggtcg tggccgcgca cttgtcgttg ccaccggcat ggcgacggag
     3181 ttcggcaaga tcgcgcgcat gctggaaggg gtagacactg cgaagacgcc gctacagaag
     3241 aacctcgatc gcatcggcaa agcgctggcc cgcgccgcga ttgccgtcgt cctgatcatt
     3301 gttgtgctcg gactttttcg cggccagcct ttcatcgaga tgttgatctt cggtgtggcg
     3361 ctggcggtgg cagttgtgcc tgaagcactg ccggctgttg tcactatctc gctcgctctc
     3421 ggcgtcaaac ggatggtccg tcgcaatgcg ctgattcgcc gtctccccgc agttgaaact
     3481 ctcggcagca cttctgtcat ttgctctgac aagacgggaa cgttgactaa agacgagatg
     3541 actgtccggc gtctttatgt agccggcaag atgattgagg tgtcgggccg aggctacgaa
     3601 ccgaacggaa agttctcgac gaacggctcg ggtgtgggaa cgtcggagcc gctatcgatg
     3661 ttgttgcagg ccgctgcgct cgcttcggac gcacgagtgg agcagagaaa tggttccggc
     3721 acgtgggaag tcaaaggtga tccgaccgag ggcgcgctgg ttgtcgtggc cgcgaaagcc
     3781 ggtctggaca aacccgaact tgacgagaag ttctcgcgga tttccgagat cccttttaca
     3841 tccgaaacga agcgaatgac cactctgcac cagacacccg acggtgttgt cgcgtacacg
     3901 aaaggcgcgc ccgaagttat tgtgcagtcg tgtgcgtcgc agttgacgga ggcgggagtt
     3961 gagccgctcg atgaagctcg cagggcagag gtgctcgagt atgcgcggca gatggcggga
     4021 gaggctttgc gtgttctggc ggtggcctat aaaccgcagg ccacggtgga agatgcggaa
     4081 aaagagatga cgctgctggg gctcgccgga atgatcgatc cgccacgtcg tgaagcaaag
     4141 gccgcagtgc gcaaatgtga agaggccggg atcaaagtca tcatgatcac cggcgaccat
     4201 ccactgacgg caaaggcggt tgccgaagag ttagggttga gcaagaacgg tcgtgttgtt
     4261 aacggtgcgg aactggaagc gatggacgac tcgcaactcg agcgggaggt cgaatcaatc
     4321 gaggtttttg ctcgcgtgtc gccggcgcac aagcttcgcg tagtgacggc gcttcagaat
     4381 cgcgggcaag ttgtcgctat gacgggcgat ggcgtgaatg atgcgcccgc gctcaagaag
     4441 gcggacatcg gaatcgcgat gggtatcacc gggactgatg tttcgaaaga agccgcggca
     4501 atgacgctga cggacgataa ctttgcgtca atcgtcgcgg ctgtcgaaga aggtcgcggc
     4561 attttcagta acatcaagaa atatttgatg tatctgttgt cgtcgaacat cggtgagatc
     4621 ggactgatgg cgggagcaag tttggcaggc ttgccgcttc cgttaaccgc ggttcagatc
     4681 ctttatgtaa atctcgccac ggatggattg cccgcgcttg cgctggcggt tgatccaccc
     4741 gagcatgggc tgatgcaacg gccgccgcgc gatccacgca caggcatctt caccaggccg
     4801 gtagtgctgc ttatggtggt gggcggcgtg tggtcggcga tcgttaatct cggtctgttc
     4861 gtgtgggctt tgaactcagg ccgtccagtt gcggaagcga tgacgatgac gttcgtatcg
     4921 ctagtcctga ttcaattttt caaagcgtat aacttccgct ccgatcgaca atcagtgttg
     4981 cgcgagccct tcgcaaacaa gtggctcaac ctggcgatcg tgtgggagct gatcctgctg
     5041 gcgttgatcc tgtatgttcc gcttttggaa aggatttttg ggaccatcgc gctgccagtg
     5101 aacgattggc tgattattgt tgccgctgca ttcaccgtgt cgcccgtgct tgagttgacg
     5161 aagtggttcg agcggaaatg gttcgctgag ctgcggtagt gaagccgttt gaaaactgag
     5221 gcgccgtctt gctggaaccg cagcgtctcg cctgcacggt gacgaattgg gcgctgccct
     5281 gagaggcagg gcgagacgcc cgcggtccca ggtggtgtct cctgtttact tttgggcccg
     5341 ttgagccgtt cacgtcacga gaagtgccgt ttgcgtattg attgttgtag cgcaacagtt
     5401 ccacagataa catgactcgc aaacggcggg tgattgttcg attcagagga gctttgtctg
     5461 aaaggtcctc tctcattctt accgggcttc agcccggtga aacggcacgc gttgtgagaa
     5521 accgctttaa aggtttcgct ggcgaatccg cgaaaaccgt tgaaacggtt cgctctgtct
     5581 taactgccct tgcaccgggc taaagcccgg tgagaatgag agagggactc ttgaggcaaa
     5641 gcaattcata ggagggcttt gcccgaaaag tattcagtgg atctctatgg aagaaattat
     5701 tctcgcgcaa aggcgcaaag gcgcaaagaa agcccttaag aaacgcggta gcgctttgcg
     5761 tctttgcgcg agatttcttg ctatagagga cttttcggcc aaagcctcat aggagagaac
     5821 tgtgagcgac ttcggagaag tggttccgat cctaaacgta aaggatcttt cagcatcgat
     5881 agattacttc gttcagcaac tcggttttga aaagaagtgg gactgggggg atccaccaaa
     5941 ctttggttgt gttggacggg gaaaggttga gatctttctg tgtcaaggct gtcagggtca
     6001 agccgggatg tggatgtcga tcttcgtcga tcaggtcgat aggctttatg aagaatacaa
     6061 ggagaacggt gcgaagatct tagacccacc aaccaattat ccctggggca ctcgcgagat
     6121 gcttgttgaa gacctggatg gccatcgttt ccggatgagc ggagagtcaa ccgaaccttc
     6181 ggcttgtgag ggaggtacag actagcgccc ggtctcaatc acggcatttc ggtcaaactg
     6241 tcgcaactca ggccaatact gcggcggtac taataaagta tggaccacgg agcgaagatt
     6301 cattgtgctg atgacgccgt aagttttcac gagattcaac atcaatttgt tcgagctcag
     6361 ttgtagcagg cgacgcacct cgactggtac gagcaatgct tgtacgcgga gtagcaggta
     6421 gtagcgccaa aatcccaggt gtcgccgata ttgctggaat aacaacgagg agtgccggct
     6481 gaaagtaaga tcgcgttgca tgtgacgccg gcgatccagt ttccactcag catatgtttc
     6541 cggcagttca ggtatcgaca aaccgttacc gattcgccgg aaaaccgcgt acagctcatt
     6601 ctgttccgac ggtgagagtg ggcgatggag caggctgtaa gcgcgttctg aataatcgac
     6661 caacatatag aggacgtcgc ggaatgccca gtcagggatc gtttgcacgc gttggcgttc
     6721 aactgcgtgg tgggctgcat tgatgcgatt aagcgtgcgc tgcgccgtct cctcgtcaac
     6781 gaacacgatg tcttgagcgt aacgcaccgt tgagaagagc cggccgattg gatcacctgg
     6841 aatcttgccg gtaaagaaga gccagtcgac cgcacggttg agggcaaact ctgcggcaga
     6901 acccgcgaag atcaaaagga tcagatccgg gtcaccccaa atcgtgcgca cgatcgagcg
     6961 cctgtcgaca aagttccgca tccgcgacct taccgcaacc gattatattt cttcatgacc
     7021 tctcgcaagc gatcatgtgg aatcgcgtag acagtatttc cgttgacgcc cgtcatcgtc
     7081 tctgccgcaa ccatggcgtt gataatggct tcttcggttg cttgcactgt agcgagaaag
     7141 accggatcga tgtgatcatt aggcagcatc ttcacattgg ctaaactcgt ctcctttccc
     7201 gcacccgcat tagcggtgga gaaagcaatg aaaatgtctc cggatgaatt gcctccgata
     7261 ccgccgtttc gcgcgatgcc cagcgctgcg cgtttcacaa cgcgtttcaa ttgatgtggc
     7321 agcagtggag cgtctgtagc gacgacgaca ataattgctc cagtatctgc cggcactcgt
     7381 tgcggcatga gatcggtaat ctcgcgacct accggcacgc cggcgatggt caacccgctg
     7441 cggcttccgt agttcgcctg gacgagaaca cctactgtgt aagtcgcagc gtctatttga
     7501 aacctgcgtg aagatgtgcc agttccacct ttaaactggt gcacgaccat gcctgtaccg
     7561 ccaccgctgt tgccttcagc aaccggcccc gacgtcgcct ggtcc
//
LOCUS       DASTEY010000608         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1513607, whole genome shotgun sequence.
ACCESSION   DASTEY010000608 DASTEY010000000
VERSION     DASTEY010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513607"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VFO72_10645"
     CDS             <1..1190
                     /locus_tag="VFO72_10645"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9479795.1"
                     /translation="DLGPLGDVTDKSLPTLCVSISVGLQVFLLPVLGALGDYSDLKKR
                     LMALFCYIAVVATCLMFFITNGYYLWGGVLFIIANVCFGAALVFYNAFLPEIATEDQT
                     DKVSSRGFAYGYLGGALLLVLNFLLVQRAEQIGISTGLAVRLSLLSAGIWWGAFALIS
                     FYLLRSRPKKKSLPAGRGYIWAGFTEIVATFKELRRLPLTVRYLIAYLIYNDGIQTVI
                     FASSAFLEQELFPGGNPSFLLQIFLFVQFTAVAGALLFERLAYLIKTKNAIIVSLVIW
                     SAIVIYAYRFLTTVAEAWVMAGAIAIVLGGSQALSRSLFSRMIPAGREAGFFGLYEVS
                     ERGTSWLGPLLFSVVIARTGSYRQALLSLIFFFVLGLVVLLITNTDKAIRQASVPSFE
                     SSS"
     gene            complement(1453..1740)
                     /locus_tag="VFO72_10650"
     CDS             complement(1453..1740)
                     /locus_tag="VFO72_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479796.1"
                     /translation="MANRKGVFIGAYVPNHLKESLRRRAAAEHRTLSQEITRILEEAV
                     HGRGLPPGSLDRRTKESQPRRRASDPMPRRRATDAPYVGSGGNRSGEERRT"
     gene            complement(1784..>2044)
                     /gene="rnpB"
                     /locus_tag="VFO72_10655"
     ncRNA           complement(1784..>2044)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFO72_10655"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          366 a          578 c          575 g          525 t
ORIGIN      
        1 tggaccttgg accgctgggc gacgttaccg acaaatcttt gccgacgctc tgcgtctcaa
       61 tctccgtcgg tttacaggtg tttctgcttc cagtgctcgg cgcgctcggc gattactcgg
      121 atttgaaaaa gcgtttgatg gcgctcttct gctacatcgc cgtcgtcgcc acctgtctga
      181 tgtttttcat cacaaacggc tattacctct ggggcggcgt gttgttcatc atcgccaacg
      241 tttgcttcgg cgccgcgctg gttttctaca acgcgtttct gccggagatc gccaccgagg
      301 atcaaacaga caaagtgtcg agtcgcgggt ttgcgtatgg ttatctgggt ggcgcgttgt
      361 tgctcgttct gaatttcctg ctggtgcagc gtgctgagca gatcggaatt tccactggac
      421 tggcggtgcg gctctcgctc ctatcagcag gtatctggtg gggcgcgttt gcgctcatca
      481 gcttctacct gctgcgatcg cgaccgaaga agaagagttt gccggccggc aggggttaca
      541 tctgggcggg tttcaccgag atcgtcgcga cgttcaaaga actgcgacga ctgccactaa
      601 cggtgcgcta tttgatcgcg tatttgatct ataacgacgg cattcaaacg gtgatcttcg
      661 cgtccagcgc gtttctcgaa caggaactct ttcctggcgg caacccgagc ttcctgctcc
      721 agatctttct cttcgtgcag ttcaccgcag ttgcgggtgc gctgctgttt gaacgactcg
      781 cttacttaat aaagacgaaa aatgcgatca tcgtgtcgct ggtcatctgg tctgcgatcg
      841 tgatctatgc ttatcgcttt ctcacgaccg ttgcggaagc gtgggtcatg gccggggcga
      901 tcgcaatcgt gctcgggggt tcgcaggcgc tctcgcgttc gctcttttcg cgcatgattc
      961 cagccggtag agaggccggc ttcttcggtc tgtacgaggt ttccgaacgc ggcacttcgt
     1021 ggttgggacc gttgttgttc agtgtggtga tcgcccgcac cggttcttac cggcaggcat
     1081 tgctctcact gatcttcttt ttcgtactgg gattagtcgt gttgcttatt acgaacacag
     1141 acaaagctat ccggcaagct tccgttcctt cgtttgagag ctcctcgtga ggcattcaga
     1201 cgagcaataa cttgccagtc ccgcgccgag gtgcagcgcc ctttccgcgg gaatccacgt
     1261 tccacaactc tcgcaacgca ccaatttgtt tttggaagga gtacgctgcg cgttcgcagt
     1321 cgtgaccgtg ccctcagtcg taacgttgag agactccacg actttccgga tcagtttgag
     1381 gtaaggacga acgcgcgagt agattattac gaaagcaata ctaacgagcg cagcgatgat
     1441 taagtatttc aatcaggttc gtctttcctc accggatctg tttccgccac tgccgacata
     1501 aggcgcgtcg gtggcgcgac gccgaggcat cgggtcagac gcgcggcggc gaggctgcga
     1561 ctccttcgtg cgccggtcca gacttcccgg tggcaggcca cgaccatgaa cggcctcttc
     1621 gaggatgcgg gtaatctcct gcgagagggt gcgatgttca gcggccgcac ggcggcgaag
     1681 cgattccttc agatgattcg gaacgtatgc gccgatgaaa actcctttgc gattagccat
     1741 ctaacgtcac cctcacttgc tcttgtcggg atttaccttg caaatacgga acaggtctgt
     1801 aagccgaatt ctgtacttcg aataattcga agtggcgatc attcatctag tccagccgtt
     1861 gccggctggc tccagcgacc tacccggagg tcgtagcctg ttaaggcttc gggcgggcag
     1921 ccctgttgac gcctccctat ttggtcttgc actgcgagga gtttgcctgg ccgcgcatgt
     1981 caccacacgc gccggtgcgc tcttacttta agcccttgcg ggccgcacca tttcacccat
     2041 cacc
//
LOCUS       DASTEY010000609         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1595962, whole genome shotgun sequence.
ACCESSION   DASTEY010000609 DASTEY010000000
VERSION     DASTEY010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595962"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFO72_10660"
     CDS             complement(<1..946)
                     /locus_tag="VFO72_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9479797.1"
                     /translation="MVFTKHKMFAPAVLTTCLLVFSASPVLAQDTTNPAGSRTVASGQ
                     KMKLKGVVTRRDADTFTVRDMNGVDTVVRLDDSTSVKTKGGFLRGGTNYAQTSILRGL
                     NVEIEGRGNATGELLAEKVRFNESDMRVARAVESRAAPLEERAENTESKLSQVEANAQ
                     RLSGQLEELAAVSNAARGGAKAAQATADAAVAGVTATNERISALDDYTPQDMAAVNFR
                     SGSAVLSTDAKATLDEIASKAMNAKGYVLEVQGFADSRGSINLNRQLSQRRADAVIRY
                     LVENHNIPLRRIVTPFGFGEMNPVAENTTREGRAQNRRV"
     gene            complement(1167..2489)
                     /gene="sucB"
                     /locus_tag="VFO72_10665"
     CDS             complement(1167..2489)
                     /gene="sucB"
                     /locus_tag="VFO72_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HET9479798.1"
                     /translation="MSTEVVMPQMGESIAEGTITKWLKKVGERVERDEPLFEISTDKV
                     DAEIPSPAAGTLTEVRFKEGDTVEVNTVVAVLDGDGSSPSVKSVSEEPAKVEPPPPPA
                     PPPPPPPPPVETKPDEPTVEELRRTKSSPLVRKIAQEHGVDISKLEGTGVSGRVTKND
                     ILSFIESGSAAAPAPAPPRRAEAPAATGPAAPAPAEIPAPPPPKPQMGDRVEQMSTMR
                     KKIAEHMVLSRRTSAHVTTVYEIDMTRVAKLREQHRDSFYERTGTKLTFMPFIFEAVI
                     KGLRQFPIFNAQVSGEQIIYKQDINLGMAVAVDWGLIVPVIKRADDLSISGLARVAND
                     LADRARTKQLKPDEVTGGTFTITNPGVFGGLFGTPIINQPQLAILGVGKIEKRPKVMT
                     SPEGDDFLAIRWLAYFALSFDHRVIDGADAERFLAFVKEQLETGDFSV"
     gene            complement(2519..>2768)
                     /locus_tag="VFO72_10670"
     CDS             complement(2519..>2768)
                     /locus_tag="VFO72_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9479799.1"
                     /translation="PTILASVKKTSKAIVLHEASRTGGIGGEIAAMLAEEAFEWLDAP
                     VVRVASIDTPVPYSPPLEDYYLPQTKDVIEAARKLAAY"
BASE COUNT          512 a          833 c          766 g          657 t
ORIGIN      
        1 caacgcgccg attctgcgca cgtccttcac gtgtcgtgtt ctccgcaacc gggttcattt
       61 cgccaaagcc aaacggcgtc acgatacggc gcagcggaat gttgtggttc tcgaccagat
      121 aacgaatgac cgcatcagcc ctgcgctggc tcagttgccg attgaggttg atgctgcctc
      181 gcgaatccgc aaagccctgc acttcgagga catagccctt ggcgttcatc gccttgctcg
      241 caatctcatc gagggtggcc ttcgcatccg tcgaaagcac tgcgctgccg gaacggaagt
      301 tcacggctgc catgtcctgt ggtgtgtagt cgtcaagcgc cgagatgcgc tcgttggtag
      361 cggtgactcc ggcgaccgct gcgtcagcag ttgcctgagc tgccttggcg ccgccacgcg
      421 ctgcgtttga aacagccgct agctcttcca actggcccga cagacgctga gcgttggctt
      481 caacctggct gagtttggac tcagtgttct cagcacgctc ctcgagtggc gccgcacgtg
      541 actcaaccgc acgcgcaacg cgcatatccg actcattgaa tcttactttc tccgcgagca
      601 gttcaccggt agcattgccg cgtccctcaa tctctacgtt caagccacgc aggatgctgg
      661 tctgggcata gttggtgccg ccacgaagga acccaccttt agtcttcacg ctggtactat
      721 catccaggcg taccaccgta tctacgccat tcatgtcgcg caccgtgaaa gtgtcggcgt
      781 cacgtcgggt gaccacgcct ttgagcttca tcttctggcc gcttgcgact gtgcgcgagc
      841 cggccgggtt ggtcgtatcc tgagccagaa ctggggaggc cgagaagact aacaggcagg
      901 tagtaagcac tgccggggca aacattttat gtttagtgaa taccatcgat ttatctcctt
      961 gtcgcttagc taatgtgacg cgcccgaacg gcgcgccttc ttcttcttgg cgagcttttc
     1021 gcgactcgcc tcccgctcgt caaattttgc ggatgacatc tcttagacct taccagaagt
     1081 taagtcaata gcctccccta ttcttcatgt aatcaccact ttatcgcaat gaactccgcg
     1141 tttgtgtggt ttgcgtctgt cgaaaactac acggaaaaat cacccgtttc caattgttcc
     1201 ttaacaaagg caaggaaccg ttcggcatcc gcgccgtcga ttacgcgatg gtcgaagctc
     1261 aatgcgaagt aggccaacca gcgaatcgcc aggaagtcgt cgccctccgg actggtcatc
     1321 accttcggcc gcttttcaat cttcccgaca cccagaattg ccaactgcgg ctggttgata
     1381 atcggagtgc cgaatagacc accaaagacc cccgggttcg taattgtaaa cgtgccgccc
     1441 gtgacttcgt caggttttaa ctgcttggtc ctggcccgat ccgccagatc attcgcgacg
     1501 cgagccaggc cggagatgct caagtcgtcg gcgcgtttga tgaccggaac tatcagtccc
     1561 caatcaaccg ccactgccat accgaggttg atgtcctgct tgtagataat ctgctcgccg
     1621 cttacctggg cgttgaagat cggaaactgg cgcagccctt tgatcactgc ttcgaagatg
     1681 aacggcatga acgtgagctt cgtcccggta cgttcgtaaa aactatcgcg atgttgttcg
     1741 cgaagctttg ctacgcgcgt catgtcgatt tcatagacgg tggtgacgtg cgcagacgtg
     1801 cgccttgaca ggaccatgtg ctccgcgatc ttcttgcgca tcgtgctcat ctgctcgacg
     1861 cgatcgccca tctgcggttt cggtggtgga ggcgctggaa tttcggcggg cgcgggcgct
     1921 gctggtccag tcgctgcggg agcttcggct cggcgtggcg gtgcgggcgc cggcgccgca
     1981 gcgctgcctg attcaataaa actcaggatg tcgtttttgg tgacgcggcc gctgacgccc
     2041 gtgccttcga gtttggaaat gtcgacgccg tgttcctgtg cgatcttgcg cacgagcggg
     2101 cttgatttcg tacggcgcag ttcttcgaca gttggctcgt ccggtttagt ttcgactgga
     2161 ggtggaggtg gaggtggagg tggtgctggt ggcggaggtg gttcaacctt tgccggctct
     2221 tcagacacgg atttcacgga tggtgaagaa ccatctccgt ccaaaaccgc gaccacagta
     2281 ttgacctcaa cagtgtcgcc ttccttgaac cggacttcgg ttaacgttcc ggcggcgggc
     2341 gaaggtatct cggcgtcgac tttatcggtc gagatctcga ataacggttc gtctcgttcg
     2401 acacgttcgc cgactttctt cagccacttt gtaatcgtcc cctccgcgat ggactcgccc
     2461 atctgcggca tcaccacttc agtactcata gtctgtgttc atccgtttaa tctgtggctc
     2521 aataagccgc cagcttgcgc gccgcctcga taacgtcctt cgtctgcggc aggtagtaat
     2581 cttcaagcgg cggtgaatac ggcaccggcg tgtcgatcga cgccacgcgg accaccggcg
     2641 catccagcca ctcaaacgct tcttccgcaa gcatcgcggc gatctcgccg ccgatgccac
     2701 ccgtgcgcga cgcctcgtgc aacacgatcg ctttgctggt cttcttcacg ctcgcgagaa
     2761 tcgtaggc
//
LOCUS       DASTEY010000610         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_327013, whole genome shotgun sequence.
ACCESSION   DASTEY010000610 DASTEY010000000
VERSION     DASTEY010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327013"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VFO72_10675"
     CDS             <1..1410
                     /locus_tag="VFO72_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479800.1"
                     /translation="VVLMILFVTLVATGQTANKTTNDVKIRQSMSAGGGNRMETVLYV
                     KGQRMRNESAGNIGFTTILQCDLKRTLTINEKTKTYLITPTDGMNAPAGAGDGGASSA
                     PAPQNQPQRGGIVNVTNTITDTGESKEMFGFTARRIKTSLEKKASPDACDKDQKVETD
                     GWYIDFQYSFECPGQEQKYQPTPVRPQPGCKDEIRTKTIGSAKLGFPLLVTTTVYHPD
                     GRTTSMTQEVLELSREPLSAALFDIPEGYTLAKDMQQLYGMGTTSMSASSQPTNSNTT
                     SSATTTAATTPASSPKKPGVLRIGLLLPKVQMSAGNAAQAAEGLRTTFASYLKGPNIE
                     VVTLSAMEEARQSQCDYILSASMNVKKGGSGSMFGRAIGNIAGSAAGHIPGGGSAATG
                     AARSAAITGVYTTAAIANSIKARDEVTLEYKLDQVEPAKTALANKDKAKASTDGEDVI
                     APLVQKAAGDVIATATQKQ"
     gene            1407..>2384
                     /locus_tag="VFO72_10680"
     CDS             1407..>2384
                     /locus_tag="VFO72_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479801.1"
                     /translation="MRAGLLILCILLAQLPLTGQALKGQKQAGEKREAVKLKRAPQTL
                     VMAKAFSEGKLPEPLRLPRGNIDQQAAALAKAVSLHDESSTAALYAAVLAAGFAVRDN
                     DGSVMQTSERGQGLILDSWQLAATAKLYGEDYGVMLSHLSDAFARGIPELKEAPLASV
                     VLDGIRAGANSNNQPVRFLSRFIVELGKNSEPSYDLLGNVDPTKTRLDAIQVSLILSR
                     LSGDLRAAEKPTAHHTKSTRPLPANWQSPCETTAREDLILDYNALASTSLFGILANKV
                     GGKLKKYGGAVGVANLFSTVFKFILTYSLVDVEITMDGDMLERTKSNTTH"
BASE COUNT          617 a          694 c          657 g          416 t
ORIGIN      
        1 gtcgttctga tgattttgtt tgtgacgctg gtcgctaccg gtcaaactgc gaacaagacc
       61 accaatgacg taaagattcg acagtcgatg agtgccggcg gcggtaatcg catggaaacg
      121 gttctttacg tcaaaggcca gagaatgcgt aacgagtcgg ccgggaatat tgggttcaca
      181 accattcttc aatgcgatct taaaagaact ctaaccatca acgagaagac aaaaacttat
      241 ctgatcacgc caaccgacgg catgaacgca ccggcaggag caggcgatgg cggtgcaagc
      301 agcgctcccg cgccgcagaa ccagccgcag cgtggcggca tcgtcaatgt aactaacacg
      361 atcaccgaca ccggtgagag caaagagatg tttggcttca ctgcccgtcg catcaagacc
      421 tcgctcgaga aaaaggcaag ccccgacgcc tgcgacaaag atcagaaagt ggaaactgac
      481 ggctggtaca tcgactttca gtattccttc gagtgtcccg gacaagaaca gaagtaccag
      541 cccacgccgg tgcggccaca acccggttgc aaagacgaga tccgcaccaa aaccatcggc
      601 agcgcgaaac tcggcttccc tctcctcgta accacaaccg tctatcatcc cgacggtcgc
      661 acaacctcga tgactcagga agtgctcgag ctctcgcgcg agccactcag cgcggcgctc
      721 ttcgacattc ccgaaggtta cacgctcgcg aaagacatgc agcagcttta tggaatgggc
      781 acgacgtcga tgtctgcctc cagccagcca accaatagca acactacttc gagcgcgaca
      841 accacagcag ccacaactcc agcaagcagt ccgaagaaac ccggcgtgct tcgcatcggg
      901 ttactgctgc caaaggtcca gatgagcgcg ggcaatgctg cgcaggcggc agaagggttg
      961 cgcacaacgt ttgcgagtta cctcaaagga ccaaacattg aagtggtcac actctccgca
     1021 atggaagaag cacgccagag ccagtgtgac tacatcctct ccgcctcgat gaacgtcaag
     1081 aaaggcggca gtggctcaat gttcggacgc gccatcggca atatcgccgg ctcagctgcg
     1141 ggtcacatac ccggcggggg ttcagccgcg accggagccg cccgttcggc agcaataacc
     1201 ggtgtttaca cgacggcagc gatcgccaac tcgatcaaag cgagagacga agtgacgctg
     1261 gaatacaaac tcgatcaggt tgaacccgcg aagaccgcac tcgctaacaa agacaaagcg
     1321 aaagcaagta ccgacggtga agacgtcatc gcgccgctgg tccaaaaggc tgccggcgac
     1381 gtgatcgcca cggccacgca aaaacaatga gagccggcct tttaatactt tgcattctgc
     1441 tggctcaact gccgcttacg ggacaggcgc tcaaaggaca gaagcaagcc ggcgagaaac
     1501 gcgaggcggt caagctaaaa cgcgcaccgc agactttggt gatggcgaaa gctttcagcg
     1561 aaggcaaatt gccggagccc cttcgcctgc cgcgcggcaa catcgatcaa caagctgcgg
     1621 cattggcgaa agcagtaagt ctgcacgacg agtcttcaac agcggcgctg tacgctgcag
     1681 ttcttgccgc gggtttcgcc gtacgcgaca acgatggcag cgtgatgcaa accagcgagc
     1741 gtggacaggg actaatcctc gactcatggc agttggctgc gacagccaag ctttacggtg
     1801 aagactatgg cgtgatgctg tcgcacctga gcgatgcatt tgcgcgaggc attcccgagt
     1861 tgaaagaagc gccgttagca agtgtggtgc tcgacggtat tcgtgcagga gcgaacagca
     1921 acaatcagcc ggtgcgattc ttgtcgcgct tcattgtcga gctgggcaag aacagcgagc
     1981 catcgtacga cctgctcggc aacgtagatc ccaccaagac ccggctagac gctatacaag
     2041 tcagtctgat cctgagccgt ctctcgggcg atctgcgagc cgcggaaaag ccgacagcgc
     2101 atcacacgaa atcaacgagg cctttgccgg ccaattggca gtcgccgtgt gaaacgaccg
     2161 ccagggagga tctaatcctc gattacaacg cgctcgcctc aacttcgctg ttcggaattc
     2221 tcgcgaacaa agttggtgga aaactcaaaa agtacggcgg tgcggtcggt gtcgctaatc
     2281 tcttctcaac ggtgttcaag ttcatcctga cgtattccct cgtcgatgtc gaaatcacga
     2341 tggacggtga catgctggaa cgtacgaaga gcaacaccac gcac
//
LOCUS       DASTEY010000611         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_887818, whole genome shotgun sequence.
ACCESSION   DASTEY010000611 DASTEY010000000
VERSION     DASTEY010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887818"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /gene="cutA"
                     /locus_tag="VFO72_10685"
     CDS             <1..231
                     /gene="cutA"
                     /locus_tag="VFO72_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET9479802.1"
                     /translation="PEMESVYRWEGKIERQSEILLLAKTTRAKLADLEREVRALHSYE
                     TPEIVAVPIVAGSNPYLEWLTDTLNPSTPDRL"
     gene            complement(260..1018)
                     /locus_tag="VFO72_10690"
     CDS             complement(260..1018)
                     /locus_tag="VFO72_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9479803.1"
                     /translation="MEDEEKTIGRKAPGSTQDEQLLESPQPDEFTHTDPWRVFRIMGE
                     FVEGFDELASLTRGVSIFGSARSRPDDPDYQAAMETAALLAAEGFAVITGGGPGIMEA
                     ANRGAFEAGGLSIGCNIELPFEQRPNPYQTRSLKFKYFFVRKMMFVKYSLGFIIFPGG
                     FGTFDELFEALTLIQTRKIRNFPVVLFDSQYWSGLLEWFRAAVLPGGKISEHDLSMFH
                     ITDSPQEVIEILNRSQNSLTEIDKLVSDDIRVPS"
     gene            complement(1035..1799)
                     /locus_tag="VFO72_10695"
     CDS             complement(1035..1799)
                     /locus_tag="VFO72_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET9479804.1"
                     /translation="MLQNKNGIIFGVANKRSIAWATAQAMHEAGARLAFTYQGDRLKE
                     NVEGLTNETMPGSLLLSCDVTKQDEVDETFRRVGEEFGRLDFLIHSIAFAPREALGGE
                     YLDTSREAFLTALEISAYSLAQLSRAAAPLMTDGGSIVTMSYYGAEKVVAGYNVMGVA
                     KAALEASTRYLAKDLGPRNIRINAISAGPIQTLSARGVSDFSTMLKHHAERAPLGRNV
                     EAREVGNTAVFLCSSIASGITGEVIYVDCGYNIMGI"
     gene            complement(1819..2775)
                     /locus_tag="VFO72_10700"
     CDS             complement(1819..2775)
                     /locus_tag="VFO72_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9479805.1"
                     /translation="MADNENSIRCRYCGQRNQVRLDSGDARCGRCRLPLSEAPHKKFA
                     RLDKDQYIHPADRRALAALRQIPGIDTALKKLLAITGESAIRVIFTASAVKVTPTQCP
                     DLYAKLQIACTTLGVDMPELFVQQNPIVNAFTGGVERPVIVLYSQLIERLSDDEVLAV
                     VAHEVGHIHAEHVLYLTAARLLEFLAKTAVLASPLALIVKEVLTISMRAALLAWARKA
                     ELSCDRAALLVTQDANVIGRTMMKLSGGTFASRVDYDQFLAQARDFQKNYDEKALDRF
                     WADAITSGLSHPFPVWRVSEILQWVESGEYKALMTSPESAAA"
BASE COUNT          569 a          779 c          864 g          618 t
ORIGIN      
        1 ccggaaatgg aatcggtcta tcgttgggaa ggaaagatcg agcgccagag cgagatcctt
       61 ttactggcga aaacaacccg cgcaaagctt gcggacctgg agcgagaagt gcgcgcgctg
      121 cacagttacg aaacgcccga gatcgtggcc gttcctatcg tcgcgggctc caatccctac
      181 cttgaatggc tcaccgatac cctgaaccct tcaactcctg accgactctg acttgtgctg
      241 cttgtgcctt tttgtgactt caactgggga cgcggatatc gtcagaaacg agtttgtcga
      301 tctcggttag tgagttctga ctgcgattca ggatctcgat tacttcctgc ggcgagtcag
      361 ttatgtgaaa catgctgaga tcgtgctcgg atatcttccc gcccggcagc accgccgctc
      421 taaaccactc cagtaatccg ctccagtatt gagagtcgaa cagcacgacg gggaagttgc
      481 gaatctttct cgtctgaatc agcgtgagtg cttcgaagag ttcgtcaaac gtgccgaagc
      541 cgccggggaa gatgatgaag ccgaggctgt acttcacaaa catcatcttg cggacgaaga
      601 agtatttgaa cttgaggctg cgggtttgat acgggttcgg acgctgttcg aacggcagtt
      661 cgatgttgca gccgatggag aggccgcccg cttcgaacgc gccgcgatta gcggcttcca
      721 tgattcctgg tccaccacca gtgatcacgg cgaagccttc agccgctaac aaagcggcag
      781 tttccatggc ggcctggtaa tcaggatcgt cgggtcttga gcgagcggag ccgaagatgg
      841 agacgccgcg agtgagtgag gctagttcat cgaacccttc gacgaattcg cccatgatgc
      901 gaaacacccg ccagggatcg gtgtgcgtaa actcgtccgg ttgcgggctc tcgagcagtt
      961 gctcgtcctg cgtggagccg ggcgccttgc ggccgatggt tttttcctcg tcttccatgc
     1021 gtctatcagc ggcgctagat gcccatgatg ttgtaaccgc agtcgacgta gatcacttct
     1081 ccggtgattc ccgacgcaat cgagctgcaa agaaacaccg ccgtgtttcc gacttcgcgc
     1141 gcttcgacat tgcgtccgag cggcgcgcgt tcggcgtggt gcttcaacat cgtgctgaaa
     1201 tctgagacgc cgcgggcaga gagcgtttgg attggaccgg cgctgatcgc gttgatgcga
     1261 atgttacgcg ggccgagatc tttggcgagg taacgcgtgc tggcttcgag tgcggctttc
     1321 gccacgccca tgacgttgta accggcgacg actttttccg cgccgtagta agacattgtc
     1381 acgatgctgc cgccgtcggt catcaacggg gccgcggcgc gtgagagttg tgcgagcgag
     1441 tacgcgctga tttcgagtgc ggtcagaaac gcttcgcgac tcgtgtcgag atactcgccg
     1501 ccgagtgctt cgcgtggtgc gaaagcgatg ctgtgaatca ggaagtcgag ccggccaaac
     1561 tcctcgccta cacgacgaaa cgtttcgtcg acttcgtcct gcttggtcac atcgcacgac
     1621 agcaacaacg aacccggcat ggtttcgttg gtcaggccct cgacattctc tttaagacga
     1681 tcgccctgat aagtgaaggc gagtcgcgcg cctgcctcgt gcatcgcctg ggcggtggcc
     1741 caggcgatgg agcgcttgtt ggccacgccg aaaatgattc cgtttttgtt ttgaagcatg
     1801 aagttgtctg tttgcagctt aagcggctgc gctttcaggt gaagtcatga gagctttgta
     1861 ttcaccactt tcgacccact gcaaaatctc cgagacgcgc cacaccggga acgggtggct
     1921 caagcctgac gtgatggcgt cggcccagaa acgatcgagc gccttctcgt cgtagttctt
     1981 ctggaagtcg cgcgcctgcg cgaggaattg atcgtaatcc acgcgcgaag caaaggtgcc
     2041 gccgctgagt ttcatcatcg tgcggccgat gacgtttgca tcctgcgtga cgagcagcgc
     2101 tgcgcgatcg caggagagtt cggccttgcg ggcccaggcg agcagcgcgg cgcgcatact
     2161 tatggtgagc acttccttga cgatcagcgc cagcggcgag gcgagcacgg cggtttttgc
     2221 gagaaactcg agcaggcgtg ctgcggttaa atagagaacg tgttcggcgt gaatgtggcc
     2281 gacttcgtgg gccacgaccg cgagcacttc atcgtcactc aaacgctcga tcagttgcga
     2341 atacaacacg atcaccggac gttcgacgcc gccagtgaac gcgttcacga tcggattttg
     2401 ttgtacgaac agttccggca tgtcgacgcc caatgtagtg cacgcgatct gcaacttggc
     2461 gtagagatcg ggacactggg tcggcgtgac cttcacggcg ctggcggtga agatgacgcg
     2521 aatggccgac tcgcctgtga tggcgagaag tttcttgagc gcggtatcga tgcccgggat
     2581 ttggcgcaac gcggcgagcg cgcggcgatc cgcgggatgg atgtattgat ccttgtcgag
     2641 acgcgcaaat ttcttgtggg gcgcctcgga gagcggcaaa cgacagcggc cgcagcgagc
     2701 gtcgccggaa tccaaacgga cctgattgcg ttgaccgcag taacggcagc ggattgaatt
     2761 ttcgttgtca gccatgttgg tgctcataat atcagtgttt gaaatgcctc acacctgtaa
     2821 agaccatcgc
//
LOCUS       DASTEY010000612         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_508290, whole genome shotgun sequence.
ACCESSION   DASTEY010000612 DASTEY010000000
VERSION     DASTEY010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508290"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /gene="ftsH"
                     /locus_tag="VFO72_10705"
     CDS             <1..1683
                     /gene="ftsH"
                     /locus_tag="VFO72_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9479806.1"
                     /translation="KAAREVVNGTPRVPKVEEEPSGSFVWPMLITWAPLLFIIAIWIF
                     MLRQMQSGGNKALSFGKSRAKLLNNQQKRVTFKDVAGVEEAKEELQEIIEFLKEPQKF
                     QKLGGRIPKGVLMMGPPGTGKTLLARAIAGEANVPFFSISGSDFVEMFVGVGASRVRD
                     LFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNDGVIL
                     IASTNRPDVLDPALLRPGRFDRRVVVSRPDVRGREGILKVHTRKIPLGEDVDISVIAR
                     GTPGFTGADLANLVNEAALNAARYNKKLVAMGDFELAKDKVLMGAERKSMVISNEEKR
                     VTAYHEAGHTLVGLKVPNADPVHKVTIIPRGMALGVTQQLPEGDRHNYSQEYLLGQIA
                     ILMGGRIAEETFLGSITTGASNDIERATELARAMVCEYGMSEMGPLTFGKKEEQIFLG
                     REIAQHRDFSEDTAIKIDQQVKKIVTAQFERAKSIIEENRETMIRLAECLLERESLDG
                     VEIRRIVAGLPLDENPPVSDGGDQDRPQLKEPTPKPLKPILPPITGGNPQPA"
     gene            1720..2553
                     /gene="folP"
                     /locus_tag="VFO72_10710"
     CDS             1720..2553
                     /gene="folP"
                     /locus_tag="VFO72_10710"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET9479807.1"
                     /translation="MPIDERTLIMGILNVTPDSFSDGGQFLSVDQALAHAEQMIAEGA
                     DIIDVGGESTRPGGEPISVEEEIGRVVPVIEALSSRVNTPLSVDTTKSEVARVALDAG
                     AAIVNDISALRFDFYVADAVARAGAGLVLMHSRGTPATMHRLPPVADIMQEVVSSLRA
                     SVHMAERRGVKRESIVIDPGIGFGKTQEQNIELIARLDELIAAFPDYPLLIGTSRKSF
                     IGRMLADGSDTPAPASHRLHGTMATITAAVLKGAHIVRVHDVKAGRETVRVSQSLKRC
                     V"
BASE COUNT          564 a          739 c          801 g          552 t
ORIGIN      
        1 aaggcagcgc gtgaggttgt gaacggaacg ccgcgcgtac cgaaggttga agaggagccg
       61 agcgggagtt tcgtctggcc catgttgatc acttgggcgc cgctactgtt cattatcgcc
      121 atctggattt tcatgttgcg gcagatgcag tcgggcggga acaaggcgtt gtcgttcggc
      181 aagtcgcgcg cgaaactgct caataatcag cagaagcgcg tcacattcaa ggacgttgcg
      241 ggtgttgaag aagccaagga agagctccag gagattattg agtttctaaa agagccgcag
      301 aagtttcaaa agctcggtgg ccggatcccc aagggcgttt tgatgatggg accgccggga
      361 acgggcaaga cattgctggc ccgtgcgatt gcgggtgagg caaacgttcc attcttctcg
      421 atttccggtt cggatttcgt cgagatgttt gtgggcgtgg gcgcatcgcg cgttcgcgac
      481 ctgttcgagc agggcaagaa aaacgcgccg tgcatcatct ttatcgatga gattgacgca
      541 gtgggtcgtc atcgcggcgc cggtcttggc ggtggacacg atgaacgcga acagaccttg
      601 aatcaattgc tggttgagat ggacggcttc gagtcgaacg acggtgttat cctgatcgct
      661 tcgacgaacc gtccggacgt tctcgatccg gcgttgctga gaccgggccg attcgaccgt
      721 cgcgtagttg tctcgcgtcc tgacgttcgc ggccgcgaag gtattctgaa ggttcacacg
      781 cggaagattc cgctcggcga agacgtagac atctctgtga ttgcgcgcgg cacaccgggt
      841 ttcaccggag ccgatctcgc caacctcgtt aacgaagcgg ctctcaacgc cgcacgttat
      901 aataaaaaac tcgtcgcgat gggcgatttc gaacttgcga aagacaaggt gctgatgggc
      961 gccgaacgca agagcatggt gatttcgaac gaggagaagc gcgtcactgc ttaccacgag
     1021 gccggtcata cactcgtcgg tctgaaagtg ccgaacgcag atccggttca caaggtcacg
     1081 ataatcccgc gcggcatggc gttgggtgtc actcaacagc ttccggaagg cgatcgccac
     1141 aactacagtc aggaatacct gctgggccag atcgcgatcc tgatgggtgg ccgcatcgct
     1201 gaagagacgt tcctcggcag catcaccaca ggggcttcaa acgacatcga acgcgctact
     1261 gaactggcgc gcgcgatggt ttgtgagtac ggcatgtccg aaatgggccc gctcacgttt
     1321 ggtaagaagg aagagcagat cttcctcgga cgtgagattg cccagcatcg cgacttctcg
     1381 gaagacacgg cgatcaagat cgaccaacag gtgaagaaga tcgttactgc tcagttcgaa
     1441 cgcgcgaagt cgatcatcga agagaatcgc gaaacgatga ttcgcctggc ggagtgtctg
     1501 ctggagcgtg agtcacttga cggtgtcgag attcgacgca tcgtcgcggg cttgccgctc
     1561 gatgagaatc cgccggtttc cgacggcggc gatcaggacc ggccgcagtt gaaggaacca
     1621 acgccgaagc cgttgaagcc gattcttcct ccgattaccg gaggcaatcc gcagccggcc
     1681 taatcagtgg cccgggtttg gcaaattaaa caccacacaa tgcccatcga tgagcgaacg
     1741 ctcatcatgg gcattcttaa tgttacccct gacagtttta gcgacggcgg gcagtttctc
     1801 tcggtagacc aggctttggc ccacgccgag cagatgatcg ccgaaggcgc cgacatcatt
     1861 gatgtcggcg gcgagtcgac gcgtcccggc ggcgagccga tttcagttga agaagagatc
     1921 gggcgtgttg tgccggtgat cgaagcactg tcttcgcgcg tcaacacacc attgtccgtt
     1981 gacacaacga aatcggaagt cgcacgcgtt gcgctcgatg ctggcgcggc gattgttaat
     2041 gacatatccg cacttcgctt tgacttttac gtcgctgacg cggtggcgcg cgcgggggcg
     2101 ggactggtgc tgatgcactc ccgcggcacg cccgcgacga tgcaccgttt accgccggtt
     2161 gcagacatca tgcaggaagt tgtcagcagt ttgcgggcca gcgttcacat ggccgagcga
     2221 cgcggcgtca agcgcgaatc gattgtgatc gaccccggta tcggctttgg aaaaactcag
     2281 gagcagaaca ttgaactgat cgcgcgcctc gacgaattga tcgctgcgtt ccctgactac
     2341 cctctcctaa ttggcacctc gcgcaaatcc ttcatcggcc gcatgctcgc ggacgggtcc
     2401 gacactcccg cccccgcctc gcatcgtctt cacggcacta tggccacgat caccgccgcc
     2461 gtcctcaaag gcgcccacat cgtccgtgta cacgacgtga aagccgggcg cgaaacagtc
     2521 cgtgtttccc aatccctcaa acgttgcgta tgatggtcac ccttatgccc aagctgctgc
     2581 gtgtgtccct tctaatcgtg atgctcgtct tcgtttccgg ttttgcggag tgctataagc
     2641 cggtgacgaa aaatca
//
LOCUS       DASTEY010000613         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_475503, whole genome shotgun sequence.
ACCESSION   DASTEY010000613 DASTEY010000000
VERSION     DASTEY010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475503"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2258)
                     /locus_tag="VFO72_10715"
     CDS             complement(<1..>2258)
                     /locus_tag="VFO72_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479808.1"
                     /translation="VVQDVRFALRQLVRTPAFTVAAVLTLALGIGANTAIFSAVNAVL
                     LTPLPFPESQQLVDLSETFKPNGFGSVSVPNFEDWKNQNTVFAGIAPYSFTSFNLEGR
                     DTPQRIPGINVGTNYFDVLGVKPTLGRAFLPGEDVAGNERVVVISDDLWRSTFAANPG
                     VVNQTIPLNGQQYTVVGVMPPELSSLYRTVQMWSPLVFPEKDRLNRDDHKYQVIGRIK
                     SDVTLAQAREQMNTIAQRLEAQYKNGRGIRLMQIEELWVASVRSPLIMMMVAVGFVLL
                     IACTNVANLLLARATVRRREISIRVALGAGRRRLIQQFLAEGLLLSVIGATIGVALAW
                     WSMGVLGKIAFPFLPRPQEIGIDSRVLLFTLAVSILTSIVFGLIPSLQAGKTDVQTTL
                     KEGGNAASESMVGGWLRQFLVVIEVAAAVVLLIGAGLMIRSVFRIREVEPGLKPQNLL
                     TAKLTLPRDKYKDAESAIRFHQQVLERVSNLPGVESAGLVSHLPIQERGFNANIGVEG
                     KTYPPNESPLVELRVISDNYFQTAGIPLLRGRVFNKQEGDGKQPVVVINEAMANQIWP
                     GEDPIGKRVQDESWATVIGIVADVKNYGLLQKPAPEMYALYTMKNFWPDMRWNLRLLV
                     RSTLDSSNIAAAVRREVQAVDPGQPIYAVQTMNLVIENTVRDKSLNTTLLSVFAGVSL
                     LLAVIGVYGVMSYTVAQHTREIGIRMALGAQPAAILKLVVGRGLVLVSAGVVIGVLAS
                     FGLTRFIEHMLFG"
BASE COUNT          486 a          682 c          633 g          457 t
ORIGIN      
        1 ccgaagagca tgtgctcgat aaaacgcgtc aggccaaacg acgccagcac gccaatcacc
       61 acaccggcac taaccagcac caacccgcgt cccacgacca gcttgagaat cgctgcgggt
      121 tgcgcgccga gcgccatccg aattccgatc tcgcgcgtat gctgcgccac cgtgtagctc
      181 atcactccgt aaactccgat cacagccagc aacagcgaaa cacccgcaaa gacactcaac
      241 agcgtcgtgt tgagactctt gtcgcgcacg gtgttctcga tcaccaggtt cattgtttgc
      301 acggcgtaga tcggttggcc tggatccact gcctgcacct ctcgccgcac ggccgcagcg
      361 atgttgctgc tatcaagcgt cgaacgcaca agcagtcgca ggttccaacg catgtcgggc
      421 cagaagttct tcatcgtgta cagtgcgtac atttccggag cgggcttctg caacagacca
      481 tagttcttca catcggccac gatgccgatc actgtcgccc aactttcgtc ttgcacacgc
      541 tttccgatcg gatcttctcc cggccagatt tggttcgcca tcgcttcgtt gatcacgacg
      601 actggctgtt tgccgtcgcc ttcctgtttg ttgaaaacac gaccacgcag caaaggaatg
      661 cccgcagtct gaaaatagtt gtcgctaata acacgaagct cgaccagcgg cgactcgttt
      721 ggcggatacg tcttcccttc aacgccgatg tttgcgttga agccacgctc ttggatcggc
      781 aggtgcgaaa caagacccgc ggattcgaca cccggcaagt tgctcacgcg ctcgagcacc
      841 tgctggtgga aacgaatcgc agactcggcg tctttgtatt tgtctcgcgg caaagtgagt
      901 ttcgcggtga gcaggttctg tggtttgagc ccgggttcga cttcacggat acgaaaaaca
      961 ctgcgaatca tgaggccggc gccgatcagc aaaacgaccg cggccgcgac ttcgatcacg
     1021 accaaaaact ggcgcagcca tccgcctacc atgctctcag acgctgcgtt gccgccttcc
     1081 ttgagcgttg tttggacgtc agtttttccg gcttgcagcg acggaatcag tccgaagacg
     1141 atgctcgtca ggatcgaaac ggctaacgtg aaaaggagca cgcgcgagtc gatccctatc
     1201 tcctgcgggc gcggcagaaa tggaaacgcg atcttgccga gcacgcccat gctccaccac
     1261 gccaaggcga cacctatcgt ggcgccgatt accgagagca ggagaccttc ggcgagaaac
     1321 tgttgaatta acctgcggcg ccccgcgccg agcgcgacgc gaatgctgat ctcacgccga
     1381 cgcacggttg cgcgcgcaag cagcaggttg gcgacgttag tgcaggcgat caggaggacg
     1441 aaacccaccg caaccatcat cattattagt ggcgagcgca cgctggcaac ccacagctcc
     1501 tcgatctgca tgagccgaat accacgcccg tttttgtact gtgcttcgag gcgttgcgcg
     1561 atggtgttca tctgctcgcg tgcctgcgcg agtgtgacgt ctgacttaat gcgtccgatc
     1621 acttgatact tgtgatcgtc tcggttgagg cggtccttct cgggaaagac cagcggcgac
     1681 cacatctgca cagtgcgata caaggacgat agctcgggcg gcatcacgcc tacgactgtg
     1741 tactgttgtc cgttcaaagg aatcgtctgg ttaacgacgc ccggattcgc ggcgaaggta
     1801 cttcgccaga gatcgtcgct gatcaccaca actcgctcat tgcccgcgac atcctcaccc
     1861 ggaagaaaag cgcgaccgag agttggtttc actccgagca cgtcgaaata gtttgtaccg
     1921 acgtttatgc cggggatgcg ttgcggtgtg tcacggcctt cgagattgaa actcgtgaac
     1981 gagtacggcg cgatgccggc gaagaccgtg ttctggttct tccagtcttc gaaattggga
     2041 actgacaccg atccaaagcc attgggcttg aaggtttccg agagatcgac gagttgctgc
     2101 gattcgggaa acgggagcgg cgttaggagc actgcattaa cagcggagaa gatcgcggtg
     2161 ttcgctccga tgccgagcgc gagcgtgagc accgccgcga ccgtgaacgc gggcgtcctc
     2221 accagctgcc gaagcgcgaa gcgtacgtcc tgaacgac
//
LOCUS       DASTEY010000614         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_952749, whole genome shotgun sequence.
ACCESSION   DASTEY010000614 DASTEY010000000
VERSION     DASTEY010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952749"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /gene="accB"
                     /locus_tag="VFO72_10720"
     CDS             <1..559
                     /gene="accB"
                     /locus_tag="VFO72_10720"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET9479809.1"
                     /translation="ARKEQSEPAAKASVNMEELRELIALVRENGLAELELEREDFRVR
                     LRREGLFSQGDQGSAFTVTEMLRNPLIADPAPVNANSPAAAPSPVQPSHPGAQAATAA
                     SADQDLHIISSPIVGTFYRSPSPTAEPFVKIGSNVEPESVVCIIEAMKLMNEIQAETT
                     GEVVKIYVENGQPVEYGQPLFGIRS"
     gene            618..2024
                     /gene="accC"
                     /locus_tag="VFO72_10725"
     CDS             618..2024
                     /gene="accC"
                     /locus_tag="VFO72_10725"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9479810.1"
                     /translation="MAFEPRKFKKILIANRGEIACRIIWTCKEMGIKTVAVHSDIDRD
                     SLHVRFADEAACIGPAPSAQSYLNIPAIISTAEIFNVDAIHPGYGFLAESPYFAEICE
                     ACNIKFIGPPASVIRLMGDKIAARRVMHEAGLPVVPGSPEAVTSEEEGLKLAREIGFP
                     LIVKASAGGGGRGMRIVRSEQELGKALETASTEAAAAFNDGSVYIERYIEQPRHIEIQ
                     VLADEYGDCIHLGERECSIQRRHQKLMEEAPSTVLTPELRKKMGEAAVAACKKLGYSS
                     AGTVEFLLDAQGNFYFMEMNTRIQVEHPVTEMVTLADVVRNQIRIAEGEPLGYTQGDL
                     LIVGHAIECRINAENPETFAPSPGTITAFNLPGGPGVRVDTFVYSGYQVSPFYDSLIA
                     KVIVHARTRELAIARMKRALDVMVVEGIKTTIPLHQKIMDDPKFRKGEIDTNFMEYFL
                     AKNARKNGEVVAGAARGR"
BASE COUNT          528 a          645 c          734 g          505 t
ORIGIN      
        1 tgcacgcaaa gaacagagcg aacccgcggc gaaagcttct gtcaacatgg aagagctgcg
       61 cgagctgatc gcgttggtgc gggagaacgg gttggccgaa ctcgagctcg agcgggaaga
      121 ctttcgtgtg cggttgcgtc gcgagggtct cttctcgcag ggtgatcagg gatccgcttt
      181 cacggtcacg gagatgttgc ggaatccgct gattgcggat ccggcgccgg tcaacgcgaa
      241 ttcaccggcc gcagctccct cgcccgtgca accgtcgcat ccaggtgctc aggccgcaac
      301 ggcagcatca gcggatcagg atctacacat catctcttct ccaatcgtcg gcacgttcta
      361 tcgctcgccc tctccaactg ctgaaccttt tgtaaagatt ggcagtaacg tcgaaccgga
      421 gagcgtcgtg tgcatcatcg aggcgatgaa actgatgaat gagattcagg cagagacgac
      481 tggagaagta gtaaagattt acgttgagaa tgggcagccg gtagagtacg ggcagccgct
      541 gttcggaatt cgaagttaat aaacgcctac gcgtttccct gaagtgcggt gacctgtcac
      601 cgctttatta ttttttgatg gctttcgaac cgcgcaagtt caagaagatc ttaattgcca
      661 accgtggcga gattgcctgc cgcatcatct ggacgtgcaa ggagatgggc atcaagacgg
      721 tcgcggtgca cagcgacatc gatcgcgatt cactgcacgt gcgcttcgcc gatgaagctg
      781 cctgcatcgg tccggcacct tcggcgcaga gttatctcaa cattccggcc attatcagta
      841 ccgcagagat cttcaacgtc gacgcgattc atcccggtta cgggttcctc gcggagtcgc
      901 cttatttcgc ggagatctgt gaagcgtgca atatcaagtt cattggacca ccggctagcg
      961 tcattcgttt aatgggcgac aagatcgcgg cgcggcgcgt gatgcatgaa gccggtttgc
     1021 cggtcgtgcc cgggagtccc gaagcagtta cgtccgaaga agagggtttg aaactcgctc
     1081 gcgagatcgg tttcccgctg atcgtgaaag cttctgcggg gggcggtggc cgcggcatgc
     1141 gcatcgttcg ttccgaacag gaattgggca aagctctcga gaccgcatcc accgaagcgg
     1201 ctgcagcatt caacgacggg agcgtgtaca tcgaacgcta cattgagcag ccgagacata
     1261 ttgagattca ggtgctggcg gacgagtacg gcgattgtat tcacctcgga gagcgcgagt
     1321 gttcgattca gcgccgccat cagaagttga tggaagaagc gccttcgacg gtcttgacac
     1381 cggagttgcg caagaagatg ggtgaggcgg cagttgctgc gtgcaagaag ctgggttatt
     1441 cgagtgccgg gactgtcgag ttcctgctcg atgcgcaggg gaacttctat ttcatggaga
     1501 tgaacacgcg catccaggtc gagcatccgg tcaccgagat ggtgacgctg gccgacgttg
     1561 ttcgaaacca gatccggatt gcagaaggcg agccgctggg ttacacgcag ggcgatctct
     1621 tgatcgttgg ccacgcgatc gagtgccgca tcaacgccga aaaccccgag acgttcgcgc
     1681 cctcgccggg aactatcacc gcattcaatt taccgggcgg acccggcgtt cgcgtcgaca
     1741 cgtttgtcta ttcgggttac caggtttcgc cgttctacga ctcgttgatt gcgaaggtca
     1801 tcgttcatgc gcgaacgcgc gaactggcga tcgcgcgcat gaagcgggcg ctcgacgtaa
     1861 tggttgtcga agggatcaag acaacgattc cgctacacca gaagatcatg gacgacccga
     1921 agttccggaa aggcgagata gacacgaact ttatggagta ttttctggcc aaaaatgcgc
     1981 gcaagaacgg agaagtggtc gccggcgctg ccaggggccg ctaaataaag ggcgtgctct
     2041 ttcctggggc cgcaggcgtc tcgcctgccc ggtgacgatt cgggcgctgc ggtgagatgc
     2101 aggcgagacg cctgcggtcc caggaaatgc gcgtccttac ttaaggacag ggtagtgtcc
     2161 taatctgaac gccagaggcg ttcgctaatt ccagcccagg gttgcttcaa ccctgggtcg
     2221 aacgttagcg tccggtccta acgctgaagg cgttggcaaa ttcgcggtta gccaacgctt
     2281 tcagcgtaaa aggtaaaaac atccaatcag ttccccaggg ttgtcgcaag ctccaacctc
     2341 tgggctggat tcgataacgc gttcggggtt cagattagga caccaccttc agattaggac
     2401 accacctaag ga
//
LOCUS       DASTEY010000615         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_426119, whole genome shotgun sequence.
ACCESSION   DASTEY010000615 DASTEY010000000
VERSION     DASTEY010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426119"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1110
                     /locus_tag="VFO72_10730"
     CDS             184..1110
                     /locus_tag="VFO72_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET9479811.1"
                     /translation="MGVDGGGTKTDAVIMDAEQRVIGEGLAGPSNPLRVGIAGAAAAV
                     REAIDSACASAKLRRNDLVAAQIGLAGARRRELRERMRETLTPLGIGEVEVVTDANIA
                     LYGATDGAPGLVVIAGTGSICCGINARRRRFCAGGWGPIAGDEGGGAWLARRALRAVA
                     YASDGRGPKTLLSELAAAYFHVTDPDDLTTAIYAPTITNERLAGFGRDVVDAAKRKDP
                     VALQIVADGGTELGRAAVAVIRHLQMERERFQVAYIGGVFRSAGDLMLDPLRAEVSKV
                     APRAYFEPPHFAPAVAAARMALERINHMALAV"
     gene            1117..1953
                     /gene="murQ"
                     /locus_tag="VFO72_10735"
     CDS             1117..1953
                     /gene="murQ"
                     /locus_tag="VFO72_10735"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET9479812.1"
                     /translation="MTNETGGALPITEQDNPRSQQLSSKSSREIVELMNTEDATVAAA
                     VQRVLDDIAKAVDETVARLRGGGKLYYVGTGTSGRLGVLDASECPPTFGVSPELVQGV
                     IAGGYDACYRAVEASEDDADAGGVDLQRRGLTAKDVVVGIAASGRTPYTVGAVTYARS
                     LGAFTVALTCVPDSPITHAAELSIVPVVGPEVLTGSSRLKAGTAQKMVLNMISTATMV
                     GLGYVSGNRMSNLQARNMKLRDRALRIVMAEAGATMDEAIKALELSGWKIAGALKRLK
                     TT"
     gene            2067..3596
                     /locus_tag="VFO72_10740"
     CDS             2067..3596
                     /locus_tag="VFO72_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET9479813.1"
                     /translation="MKALDLIIIFGYLIGIVLFGTWFGRKQKTTSDYFLGDRSVPWWA
                     VAFSIVATETSTITFISVPGIAFARGGNFQFLQLVFGYLLGRVVISILFIPSYFRGEL
                     LTVYQLLDRRFGGKIKMLAASLFIVMRNIADGIRLLLTAIVLAAVYAAFQPGANVETI
                     TIASIVLIGVAMIIFTYFGGMEAVIWVEVVQLGIYIAGAIAAAVVLINSIEGGFAGAS
                     ALARQFGKYSVLDFAFDYTKTYTFWSGLIGGCFLTMSTHGTDQYLVQRYLCTNRSRHA
                     TIALLTSGAIVLVQFIGFLFVGVLLFAFYQPFTDPAYATAASATFPFTSGDRVFPDFI
                     TKHMPSGLSGLVVAAIFAAAMSSSLNSIAATAVNDLYKPFRPARSDKHYLKVSHLLTL
                     IWGVVQIGVAFAVRNQPGSALSKALSIASLINGPILGVFLVGTFLRRVSQPPALIGML
                     VSIVSMLYIFFLRPEIAWTWYVLLGSSITFVVAWLSSFAFRPAPAEMRDELSPVVATE
                     D"
     gene            3685..>5124
                     /locus_tag="VFO72_10745"
     CDS             3685..>5124
                     /locus_tag="VFO72_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9479814.1"
                     /translation="MLRLNRYVSLALMLVLMLALAALPARSQEAALVKPPKVVAFKRQ
                     PSAAALKWANEELRRMSLEEKVGQLISVGVNATFLNQESEAFRALKHHIEENKVGGII
                     LFRGPVYESVILVNRMQQLARYPLLISADLEAGAGMRFEDTINFPWNMAVAATGNPEY
                     ARRQGEITAREARALGVHQIFAPVVDVNNNAANPVINVRSYGEDPAEVARFAAAFTEG
                     AQAAGVIATAKHFPGHGDTAVDSHRGLPEINVGRDRLNTVEFVPFQATVNAGVGSVMV
                     GHIALPQIDATAIKPLPRDVKGKPIDTSEEGEIIDEKATIPATLSPVMGQILRNDLKF
                     PGMIVTDALSMSGLTIYFTQEEAAVRALEAGADMLLKPADVDAAFRGVRDAVKSGRIS
                     EGRVGESARKIMAAKYDLGLVEQRLTPIDTIDRVVGSKDVVKLATDIAEHAVTLVRDD
                     DKLVPLKDLKPDAKIFNLAITNGEDRNWIA"
BASE COUNT         1040 a         1381 c         1586 g         1117 t
ORIGIN      
        1 acatatcaag atgggttatc acaataataa aatcttttag tttggattat aaatcactgc
       61 ctcctagaat gatcgaaaaa tgttcaccgt tttttctggt ccagccgggc cccgattgtg
      121 agcacagagc gacgcacagc agatctccct ccagccagac gcctgcacac ccaaaattcg
      181 gtggtgggtg tcgacggcgg cggcacgaag accgacgccg taatcatgga cgccgagcaa
      241 cgggtgattg gcgaaggcct ggctggtcca tcgaatccgc tgcgcgtcgg tatcgccggc
      301 gccgccgcgg cggtgcgcga ggcgatcgat agcgcctgtg cgtcggcgaa actccggcgc
      361 aacgatctcg tcgcggcgca aatcggactg gccggggcga ggcggcggga gttgcgcgaa
      421 cgcatgcgtg agacactcac tccgcttggc atcggcgagg tggaagttgt aactgacgca
      481 aatatcgctc tgtatggcgc gacagatggt gcgcccggac tcgtcgtgat cgctggaacc
      541 ggatcgatct gctgcggcat caacgcgcgg cggcgacgtt tctgtgccgg tggttgggga
      601 ccaatcgccg gcgacgaagg tggtggagca tggttggcac ggcgggcgct gcgcgcggtc
      661 gcttatgcgt ctgacggacg tggtccaaag acgctgttgt ctgagctggc agcggcatat
      721 tttcacgtca ccgatccgga cgatctgacc actgcgatct acgcgcctac gatcacgaac
      781 gaacggctgg ctggttttgg acgagacgtt gtcgacgctg ccaagcgaaa ggatccggta
      841 gcattgcaaa tcgtcgctga cggcggcacc gaactcggcc gggcggcggt cgcggtaatt
      901 cgccatttgc agatggagcg cgaacgcttt caggttgcgt acattggcgg agtatttcgc
      961 tcggccggag atctgatgct cgacccgctg cgcgccgaag tgagcaaagt cgcgccgcgc
     1021 gcgtatttcg agccgcccca cttcgctcca gccgtcgctg ccgctagaat ggcgctcgaa
     1081 cgcatcaatc acatggctct cgcagtttga taccagatga ctaatgaaac cggcggcgcg
     1141 cttccgatca ccgaacagga caatccacgt tcacagcagc tgagttccaa gtcgtcgcgc
     1201 gagatcgtcg agctgatgaa tacggaagat gcgacggtcg cagccgcagt gcaacgcgtc
     1261 cttgacgaca ttgcgaaagc tgtcgatgaa acagttgcga ggttgcgcgg aggcggaaag
     1321 ctttattacg tcggcaccgg cacgtccgga cggctgggag tgctcgatgc gtctgaatgt
     1381 ccgccgacat ttggtgtgtc gcccgagctg gtccagggtg tgattgcggg cggatacgac
     1441 gcgtgttacc gcgcggtgga agcgtcggaa gatgatgcag acgctggcgg cgtggatttg
     1501 cagcgacgag ggttgactgc taaagacgtg gtagttggca tcgccgcgtc cggaagaacg
     1561 ccttacaccg ttggagcagt gacatatgcg cggtcgctgg gtgcattcac ggttgctttg
     1621 acgtgcgttc ccgattcgcc gatcacgcac gcggctgaac tcagcattgt ccccgttgtt
     1681 ggaccagaag tgttgaccgg ttcaagccgt ttgaaagccg gaacggcaca gaagatggtg
     1741 ctgaacatga tctccactgc gacgatggtt ggactgggat acgtgagcgg caatcgcatg
     1801 tccaacttgc aggcgcggaa tatgaagttg cgcgatcgcg cgctgcggat agtgatggct
     1861 gaggccggag ccactatgga tgaggctata aaggcgctgg aactctccgg ttggaagatc
     1921 gccggcgcgt tgaagcgcct caagacaacg tagcgtttct tccaagggat cgccaggcgt
     1981 cccgcctgcc ttaccggccg cagagggctt tctgcgccag catgcaggcc ggacgcctgc
     2041 ggtcccagga aagagcccgt aaataaatga aagctctcga tctcataatc atcttcggct
     2101 acctgatcgg gattgttctg tttggcacct ggtttggacg aaagcagaag acgacgagcg
     2161 actacttcct cggcgatcga tcggtgccgt ggtgggccgt tgccttttca atcgtcgcta
     2221 cggaaacatc gacgatcacg ttcatttcgg tgccgggtat cgccttcgca cgtggcggaa
     2281 acttccagtt tctccaactt gttttcggct atctgctcgg acgagttgtc atttcaatac
     2341 tcttcatccc gtcgtacttt cgtggtgaac tgctgactgt ctaccaactg ctcgaccggc
     2401 gttttggcgg caagatcaag atgcttgccg cgtcgctttt tatcgtcatg cgcaacatcg
     2461 ctgacgggat acgtctgctg ctaaccgcca tcgtgctggc ggcggtctat gcggcgtttc
     2521 aaccgggtgc aaacgtcgaa acgatcacca tcgcttcaat cgtgctgatc ggcgtggcga
     2581 tgatcatctt tacttacttc ggcggcatgg aagcggtgat ttgggtcgag gtcgtgcagc
     2641 tggggatcta catcgccggt gcgattgccg cagcggtggt gctgatcaat tcgatcgaag
     2701 gtggatttgc aggtgcaagt gcgctcgcga ggcagtttgg caagtattca gtgttggact
     2761 tcgctttcga ttacacaaag acttacacgt tctggtcggg actgatcggc ggttgctttc
     2821 tgacgatgag cacgcacggc acggatcaat atctggtgca aaggtatctc tgtacgaatc
     2881 ggtcgcggca cgccacgatt gcgttgttaa caagcggcgc gattgtgttg gtccaattca
     2941 tcggatttct gttcgtgggc gtgctgctgt ttgctttcta ccagccgttt acggaccccg
     3001 cgtatgcgac tgcggcttcg gctacatttc cattcacgag cggcgaccgc gtgtttcccg
     3061 actttatcac caaacacatg ccgagcggtc tttcgggttt agttgtggca gcgatctttg
     3121 cggctgccat gtcgtcgtcg ctcaactcga ttgcggccac ggcggtaaac gatctctaca
     3181 aaccgttccg gccggcgcga agtgacaagc actatctgaa agtctcgcat ctgctgacgt
     3241 taatctgggg cgtcgtgcaa ataggcgtgg cgttcgcagt aagaaatcaa ccggggtcgg
     3301 cgttaagtaa ggcgctttcc atcgcttcac taattaatgg gccgatactt ggcgtctttc
     3361 tcgttggcac gttcctgcga cgcgtcagtc aaccgcccgc gttgatcggc atgctcgtga
     3421 gtatcgtgtc gatgctctac atattcttcc tcaggccgga gattgcgtgg acgtggtacg
     3481 tgttgctggg aagttcgatt actttcgtgg tggcgtggct ctcgagtttt gctttccggc
     3541 cggcgcctgc cgagatgcgg gacgaattgt cgcccgtagt tgctactgaa gactagttcg
     3601 gaaatctctg tgactctgtg cctctgtggt tttccaccac aaagacacag aggcacagag
     3661 caagaggggg aaaccaagcg agtcatgctg agactgaatc gttacgtttc attggcgctg
     3721 atgctggtgc tgatgctcgc actggctgcg ctacccgcgc ggtcgcagga agcggcgctt
     3781 gtaaagccgc ccaaggtggt cgcgttcaaa cggcaaccgt cggcagcggc gttgaagtgg
     3841 gccaacgaag aactgcggcg catgtcgttg gaagagaagg ttggtcagtt aatttccgtc
     3901 ggcgtgaatg caactttcct gaaccaggaa tcggaagctt tccgcgcgct caagcatcac
     3961 atcgaggaga acaaagttgg aggcatcatc ctctttcgcg gaccggttta cgaatccgta
     4021 atcctcgtaa atcgaatgca gcagttggct cgctacccgc tcttgatctc tgccgatctc
     4081 gaagcgggcg cggggatgcg ctttgaagac acgatcaact ttccctggaa catggcggtc
     4141 gctgccacag gcaatcctga atacgcgcgg cgtcaaggcg agatcaccgc acgtgaagct
     4201 cgcgcactcg gagtacatca gatatttgcc cccgtcgtcg acgtaaacaa caacgccgcg
     4261 aatcccgtga tcaatgttcg gtcgtacggt gaagacccgg ctgaggtcgc acggtttgcg
     4321 gcggcgttca ccgaaggcgc acaggcagca ggcgtcatcg ccaccgcaaa acactttccc
     4381 ggacacggcg acaccgcggt cgattcacat cgcggtttgc cggagatcaa tgtcggacgc
     4441 gatcggctga acacggtcga gtttgttcca tttcaggcaa cggtaaacgc gggcgtcggt
     4501 tcggtgatgg tgggccacat cgctctgccg cagatcgacg ccactgctat caagccgttg
     4561 ccgcgcgacg tcaaaggcaa gccgatcgac accagtgaag agggtgagat catcgacgaa
     4621 aaggcgacga taccggccac gttgtcgccc gtgatgggac agatcctgcg aaacgatctg
     4681 aagtttccgg gaatgatcgt gacggatgcg cttagcatga gtggtctgac aatctacttc
     4741 actcaggaag aagcggcggt ccgcgcactc gaagcgggcg ccgacatgct gctgaagcct
     4801 gcggacgttg atgctgcgtt tcgcggcgtg cgcgatgcgg tgaagagtgg acgcattagc
     4861 gagggtcgcg tgggggagtc ggctcggaag attatggctg cgaagtatga tctgggcctc
     4921 gtggaacaac ggctcacacc gattgacacc atcgaccgag tcgttggtag taaagacgtt
     4981 gtaaaactcg cgaccgatat agcggaacac gcggtcacgc ttgtgcgtga cgacgacaag
     5041 ctcgttccac taaaggacct caaaccggat gcgaagatct ttaacctcgc gattaccaac
     5101 ggtgaggatc ggaactggat agcg
//
LOCUS       DASTEY010000616         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1266472, whole genome shotgun sequence.
ACCESSION   DASTEY010000616 DASTEY010000000
VERSION     DASTEY010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266472"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VFO72_10750"
     CDS             complement(<1..1053)
                     /locus_tag="VFO72_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9479815.1"
                     /translation="MKTDRLLLLAVTIVVLIYGVIYPNLSVVSASLQRAGSWTLANYS
                     EILSQQVVIEAIVSSLGLSIATVVFCALVGVPLAFLFERFTFPGRRLFAAIAALPLVL
                     PPLVGAVAFIFLFGESGILAHSIQNLFGLQNAPWQLAGWPALLLFHTYTMYPFFYVLT
                     GAGLRRIDASLAEAARSLGGSRAFVFTRVLVPQLTPSLIAAALLTFMTSMASFSAPLL
                     FGGNVRVLTLEIFTARQRGDVAIAVTETVVLAVISLAALVLFQRYEGTRKFAAATMKG
                     APRRRVTIASGKGRVLAAIAAVAFAIILVSPVATLILVSFAREGSWTTQTLPPAYTVA
                     NYQRLFGDPSAAEVFYN"
     gene            complement(1050..2126)
                     /locus_tag="VFO72_10755"
     CDS             complement(1050..2126)
                     /locus_tag="VFO72_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9479816.1"
                     /translation="MPLLSLQNISRNFGPTRAVADVSLEVERGEFFGLLGPSGCGKTT
                     TLRMIAGLEKPDSGSIRFQEADITNLPPERRGFGMVFQNYALFPHLNVFENVAFGLRA
                     RHASKQEMNESVRSALELVQLPGYEKRAIDELSGGQQQRVAIARAIAIEPVLLLFDEP
                     LSNLDVSLREETRGELRELVTRLGLTAVYVTHDQEEAFALCDRISVMVGGKLMQTGKP
                     RELYEQPADIAVARFLGRNNLIRAMRLSSSKTSDGEFKTLEGEHTLHVPVTRDDLAPL
                     NKPVVLAIRPEHVQLSSNDQTAPNSLRGIVRELVFAGATSTVRVDAGGLLLEALVLQP
                     DGLRVDQECTVILRPDKLRLLKHG"
     gene            complement(2126..>2650)
                     /locus_tag="VFO72_10760"
     CDS             complement(2126..>2650)
                     /locus_tag="VFO72_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9479817.1"
                     /translation="TMRAIFGAMIWRFYKDTGSTEGGYEWLRKLDANVHEYTADGTLL
                     MQKLARREGLVTLWDMPDVRLFKEQKNLPIEYRVPASGTPVIIDGIAIIRGAPNEEEA
                     KRFYEFVTTPESLAYAAQKYYRIPIRTDMDRSQLPAWMNEPFTRLPLDWELLRKHGAD
                     WLRYWDTQIRGRNK"
BASE COUNT          592 a          784 c          749 g          525 t
ORIGIN      
        1 gttgtaaaaa acttccgccg ctgacggatc gccaaatagc cgctgatagt ttgccacggt
       61 gtaagccggc ggcagggtct gcgtggtcca cgatccctcg cgcgcgaaac tgaccagtat
      121 caacgtggcg accggcgaca ccagtatgat ggcgaaagcc acggcggcga ttgccgccag
      181 cactcgtccc ttgccactcg cgatcgtcac gcgtcttcgc ggcgctcctt tcatcgttgc
      241 agccgcgaac ttgcgcgtgc cttcgtaacg ttggaacaga accaacgccg ccagcgagat
      301 cacagcgagc acgactgttt ccgtcaccgc tatcgccacg tcgccgcgct gtcgggcggt
      361 aaagatctcc agcgtcagca cacgcacgtt gcccccaaac aaaagcggcg cgctgaacga
      421 agccattgaa gtcataaacg taagcaacgc cgccgcgatt aatgaaggcg tgagttgcgg
      481 tacgagcacg cgtgtgaaaa caaaagcacg cgaaccgccc agactgcgtg ctgcttcggc
      541 aagactcgca tcgattcttc gcagaccggc gccagtcagc acgtagaaga acggatacat
      601 cgtgtaggtg tgaaaaagca gcaacgccgg ccaaccggcg agttgccacg gcgcgttttg
      661 tagcccgaaa agattctgaa tgctatgggc caggatgccg ctctcgccaa acaggaagat
      721 gaatgctact gcgccgacga gtggcggcag aacgagcggc agcgccgcaa tcgccgcgaa
      781 cagacgtctg ccgggaaaag taaatcgttc gaacagaaac gcgagcggca cgcccaccag
      841 cgcacagaac acaaccgttg cgatcgatag tccgagactg ctgacgatcg cttcgatcac
      901 aacttgctgc gatagaattt cgctgtagtt cgcgagcgtc cacgagccgg cgcgttgcag
      961 agacgcggat accacggaca gattcggata gatcacgcca taaatgagga ctacgattgt
     1021 tacggcgagc aagaggagcc gatccgtttt catccgtgtt tcagcagtcg caacttgtca
     1081 ggacgaagaa tcacagtgca ctcctgatca actcgaagac cgtccggctg taacaccagt
     1141 gcctcaagta ataatcctcc ggcgtcaaca cgcacggtgg acgtcgcgcc ggcaaatacg
     1201 agctcacgaa cgatgccccg cagcgaattc ggcgcggtct gatcgttcga actcagttgc
     1261 acgtgctcag ggcgtatcgc cagcaccacc ggcttgttca aaggagccag atcgtcgcgc
     1321 gtgaccggaa cgtgaagcgt gtgttcgcct tcgagcgtct tgaactcacc atcgctcgtc
     1381 ttgctcgaac ttaaacgcat cgcgcggatc agattgttgc gccccaggaa gcgcgccacg
     1441 gcaatatccg ccggctgttc gtagagctcc cgcggcttgc cggtttgcat cagttttccc
     1501 ccgaccatca cactgatgcg atcgcacaaa gcaaaagcct cttcctgatc gtgtgtgacg
     1561 taaacggccg tcagtccaag acgcgtcacc agttcgcgca gttcgccacg tgtttcctca
     1621 cgcaacgaaa cgtcgagatt cgaaagtggt tcgtcaaaca gcagcagcac cggctcgatc
     1681 gcgatcgcgc gcgcaatggc gacacgctgt tgctggccgc cggagagttc gtcgatggct
     1741 cgtttttcgt agccgggaag ttggaccagt tccaacgcgc tgcggacact ttcgttcatc
     1801 tcctgttttg aagcgtgacg ggcgcgcagt ccgaaggcga cgttttcgaa tacgttgaga
     1861 tgaggaaaga gggcgtagtt ttgaaaaacc attccaaagc cgcgccgctc gggcggaaga
     1921 ttcgtgatgt ccgcctcctg gaaacggatc gaaccggagt cgggcttctc gagacctgca
     1981 atcattcgga gtgtcgtggt cttgccgcag cctgatggcc caagcagacc aaaaaactca
     2041 ccgcgttcca cctcgagtga aacgtcggcg accgctcgcg tcgggccaaa gttacgagag
     2101 atgttttgca gcgaaagcag aggcattatt tattgcggcc acggatctgc gtgtcccaat
     2161 atcttaacca gtcggcgcca tgttttcgca atagctccca atcgagcggc agccgcgtga
     2221 acggctcgtt catccaggcc ggcaactgag aacgatccat atccgtgcgt atgggaatgc
     2281 ggtaatactt ctgcgccgcg taagcaagac tctccggtgt ggtcacaaac tcgtaaaagc
     2341 gttttgcttc ttcttcgtta ggcgccccgc gaatgatcgc aatgccgtcg atgatcacgg
     2401 gtgtgccgct cgccggcact ctgtattcga tcgggagatt tttctgctct ttgaagaggc
     2461 gaacgtcagg catgtcccac aaagtgacca gcccttcgcg gcgtgcgagc ttttgcatca
     2521 ggagcgtgcc gtcggcggtg tattcgtgca cgttcgcgtc gagtttccgc agccattcgt
     2581 agcccccctc ggttgaaccc gtatctttgt agaaacgcca gatcatagcg ccgaagattg
     2641 cgcgcattgt
//
LOCUS       DASTEY010000617         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_195412, whole genome shotgun sequence.
ACCESSION   DASTEY010000617 DASTEY010000000
VERSION     DASTEY010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195412"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1742)
                     /locus_tag="VFO72_10765"
     CDS             complement(42..1742)
                     /locus_tag="VFO72_10765"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9479818.1"
                     /translation="MNFSRRKFLSGTAKSAVAVTLLRSVVPANTLAQAAAVQPAFARL
                     DEFIARHMRETGAPGMTLALANRDGLIRTSTYGYADTKAGVRVAPETMFEIGSVSKSF
                     VGLVLLQLRDEGKLDFNKPIVEYLPWLKINSKFEAITTHHILSHTGGLPGAPLLLDAL
                     LSELWTAYEPGKRFLYSNTGYNILGFLIEAVDKRSFAESMRARMLVPLGMTASSPIIT
                     NDLRKQMAIGYEPLAEGKPFSVGGPLAEAQWIEVDIAAGSIASTPSDMAKYIRMLLNR
                     GALPKGRLLSEEAFALFTKPAINSPYRGEPASYGYGIWVSDIGGHMRLRHTGGMVAFS
                     SSIDVDVTGGIGAFASVNANLRGYRPVAVTKYAIELFNASLAGKPLPDPPAPPPAPDE
                     VKNAADYVGTYTSLDGKKLEFAAEGNKLVLVHANRRVVLDRIGPDRFIVKHPDFDAFV
                     LGFARENQQVTQAFHGANWYMGAKYNGPKTFETKKEWDAFVGHYHNDSPWYGDTRVVL
                     RKGQLFIDGVQPLVPRGDGKFGIGDPAAPDWISFESIVDGRAMRLNFSGIIFRRTFTP
                     "
     gene            complement(1751..>2283)
                     /locus_tag="VFO72_10770"
     CDS             complement(1751..>2283)
                     /locus_tag="VFO72_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9479819.1"
                     /translation="AKTAHDLEPFSLTIYSDYIRTLYWAGKLEEARKEALKLREMDAK
                     FTRAHYELGLVLEEEGKVDEAIAEFRLGLKYVPDNVAGLTALGHAQGLAGKRADAEKV
                     IARLQELSKQQYVSPFQTAVVYAGLDERQLALDWLEKSRQERFNWLPFIKIDPVFKNL
                     RSEPRFVELSKNLGLN"
BASE COUNT          521 a          661 c          608 g          493 t
ORIGIN      
        1 aagcggcgtc aagccgccgc actccaagga ggcgcccgat gctagggtgt gaaggtgcgg
       61 cggaagatga tgccggaaaa gttcaagcgc atcgcgcgcc catcgacgat tgactcaaaa
      121 ctgatccaat cgggcgccgc cggatctcca attccgaact tgccatcacc gcgtggcacg
      181 agcggctgga caccgtcaat gaagagctgg cccttgcgca acacaacgcg cgtgtcgccg
      241 taccacggac tgtcgttgtg gtagtgccca acaaacgcgt cccattcttt cttcgtttca
      301 aacgtcttcg gaccgttata cttcgcgccc atataccagt tggcgccatg aaaagcctgc
      361 gtcacctgtt gattctcccg cgcaaatccg agcacaaagg cgtcgaagtc cgggtgcttt
      421 acgataaacc gatccgggcc aatacgatcg agtacaactc tgcgattcgc atgcacgagc
      481 acaagtttat tgccttcagc agcgaactcg agctttttgc cgtcgagtga agtgtaggtt
      541 cctacgtaat cggcggcgtt cttcacttca tcgggagcag gtggaggcgc cggtggatcg
      601 ggaagcggct tccctgctaa cgacgcgtta aaaagctcga tcgcatactt cgttactgca
      661 accggtcgat aaccgcgaag gttcgcgtta acagacgcaa acgcgccgat gcctccagtt
      721 acatccacgt cgatcgaaga actgaaagcg accatcccac ccgtatgacg caaacgcatg
      781 tgcccgccta tgtcgctcac ccaaatgccg taaccatacg aagccggttc gccgcgatac
      841 ggagagttga tcgcaggctt tgtgaagagt gcaaaagctt cttctgaaag caggcggcct
      901 ttgggcaagg cgccacgatt cagcagcatg cgaatgtatt tcgccatgtc tgacggtgtc
      961 gaagcgatgc tgccggcagc gatatctact tcgatccatt gtgcttcagc cagtggacca
     1021 ccaactgaaa atggctttcc ttctgcgagt ggctcgtagc cgattgccat ctgtttgcgc
     1081 aagtcattgg taatgatcgg tgagctcgct gtcattccca gtggcacgag catgcgtgcg
     1141 cgcatcgatt ccgcgaagga gcgtttatcg acagcttcaa tcaggaaacc gagaatgttg
     1201 taaccggtgt ttgagtaaag gaaacgtttg ccgggttcgt aagcagtcca caactcactg
     1261 agcagcgcgt ccagcagcaa gggcgcgcct ggcagtccac ccgtatgact gagaatgtga
     1321 tgagttgtga ttgcttcaaa cttggaattg atcttcaacc atggcaggta ttcgacgatc
     1381 ggtttgttga aatcgagttt gccttcatcg cggagttgca acagcaccag accgacaaac
     1441 gacttcgaga cggagccgat ctcaaacatc gtttccggcg caacgcgcac acccgccttc
     1501 gtgtcggcat agccgtaagt tgacgtcctg atgagaccgt cgcgattggc gagcgcgagt
     1561 gtcatgcccg gcgcgcctgt ctcgcgcatg tggcgcgcga tgaattcatc gagccgcgcg
     1621 aacgccggtt gcacagcggc cgcctgcgcc aacgtgttcg ccggcacaac tgatcgcagg
     1681 agagtcacag ctaccgcgga cttcgcagtg ccggaaagaa attttcgacg tgaaaaattc
     1741 atagtcctct ttaattcaat ccaagatttt tcgacaactc tacaaaccgc ggttccgaac
     1801 gaaggttctt aaaaaccgga tcaattttga tgaaaggcaa ccagttaaag cgttcctgcc
     1861 gcgacttctc gagccagtcg agcgcgagct ggcgctcgtc gagcccggca tagaccacgg
     1921 ccgtttgaaa aggcgaaaca tactgctgct tcgaaagctc ctgaagcctc gcgatcacct
     1981 tctccgcatc ggcgcgcttc ccagcgagtc cttgcgcgtg gcccaacgca gtcagccccg
     2041 caacattgtc gggcacatac tttaacccaa gccgaaattc cgcaatagcc tcgtccacct
     2101 tcccttcttc ttcaagaaca agtcccaact cgtagtgagc gcgcgtgaat tttgcgtcca
     2161 tttcgcgaag cttcagcgcc tctttgcgcg cctcctcaag cttgccggcc cagtaaagag
     2221 tgcgaatata gtcgctgtag attgttaacg agaacggctc gagatcatgc gcggttttgg
     2281 cct
//
LOCUS       DASTEY010000618         5033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_607045, whole genome shotgun sequence.
ACCESSION   DASTEY010000618 DASTEY010000000
VERSION     DASTEY010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607045"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFO72_10775"
     CDS             complement(<1..460)
                     /locus_tag="VFO72_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9479820.1"
                     /translation="MAFKSIFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     VTNQVEAVGRDAKDMLGRTPGNIVAIRPMKDGVIANFEVTEKMLQHFIRKAHNGRTWV
                     SPRVVIGIPSEITQVERRAVEDSAYRAKASEVYLVEEARAAAIGAGLPITE"
     gene            complement(902..2500)
                     /locus_tag="VFO72_10780"
     CDS             complement(902..2500)
                     /locus_tag="VFO72_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET9479821.1"
                     /translation="MSSHVILLTDAHEAPAYELIEALRAAGVKTLIEGLREVEVEVAL
                     ARQQKSHGGLSDLDGPPPLAVLYEVVPGADMVELHTAVNHAKAFWPGAPLVACRRQMN
                     GYQSLNLRSLDGATLKRLGFLAIADKSAQLPALLREIEGQGTSGELNLPEVVERPAGR
                     GCSSLPPKIKFNDLRAAFDLVASLHFIGDQSGAANTALAGLEQLIDADRWAIYLFSEE
                     KGIESSTLEAIAVRRPGQQGSGSRSEEEWRRVLTGESDLPLGSETKAAKRAAAGMGII
                     KKKERGQFVLAVPLICGERILGVIEGIRQGAGARAFKKTDVALLDSLSLPIASALANA
                     VRIAEAERLSQTDDLTKLHNARYLRQFLLNEIRRARRYGTSVAALFLDLDDFKRINDE
                     HGHLVGSHVLMEMAAVILSSIRDTDAVARYGGDEFVIVLPDTGTELAGTVAERIRQKI
                     LRHQFTGGRRLQLSMTASFGVAAFPKHASSPQQLIACADTAMYEAKAANKNCVRFAAD
                     LAPNEPPKQVIELLEVDAGSVLQG"
     gene            complement(2518..3771)
                     /locus_tag="VFO72_10785"
     CDS             complement(2518..3771)
                     /locus_tag="VFO72_10785"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9479822.1"
                     /translation="MVANRTSRFAFDWWIIQKRFVYLVIAIFLLCGVAAGAALYVWKY
                     GNPFKNVAQVNYPAGARFISFEGDVRVIRAATREIIPANADTQLYPGDTVQTQASGRA
                     RIGLADGSTLVVKPNSTIIVRDNARADDGKRTNVHVHVESGQLSVRTEQQADGTTNVV
                     ETPKTKNTVGESTNASFGVNAEGTEEIRVSTGVIETTNRSGEKASIRGGEYVSVNNSG
                     RLSAAQKLLDVPQPTTPDDLEKVFVGANGAASVALRWQRPQSGTPAYYRVEVATSPFF
                     VADGKVIERDQLAATEFGASDLRPGVYFWRVRATDASGQASDWSDPLKFIVATRGTGS
                     QVAVSNLTAELLGGSLYLVRGRAEPGTTIKVTGRETIVAPDGSFQIQVTAPGGAREVS
                     IEAQDPQGNSTQYRVSLSGRNTRRT"
     gene            complement(3963..4988)
                     /locus_tag="VFO72_10790"
     CDS             complement(3963..4988)
                     /locus_tag="VFO72_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9479823.1"
                     /translation="MDIFSGGSTWLWVGFSIFIVTMLSLDLGLFNRKAHTIRYREAWI
                     WSTAWVTLALIFAGLVFRYQGSVRGLEFLTGYLIELSLSVDNLFVFLLIFSYFKVPAQ
                     FQHRVLFWGVLGALIMRLTMIFIGAALINRFHWIIYIFGAFLVYTGIKMFRHEEVDIN
                     PEHNIAVRAVTRVFPISRHYSGEKFFTRVNGKRTGTLLLLVLMVVEVTDLVFAVDSIP
                     AIFAITRDTFIVYTSNVFAILGLRSMYFLLAGVVEKFRFLRTGLAIVLTFIGIKMLIG
                     AIGFHIPILFSLAFVAVVLLGSVVFSLIYPGKPEMPIEVDLPADFANPDPGEEVEDPT
                     EVEIKRA"
BASE COUNT         1092 a         1408 c         1415 g         1118 t
ORIGIN      
        1 gctcggtgat cggaagtccg gcgccaatcg ccgcagccct agcttcttcg acgaggtaaa
       61 cttccgacgc cttggcgcga tacgcggagt cttcgactgc gcggcgctca acctgggtga
      121 tctcactcgg aattccaatc acaactcgcg gactaaccca cgtgcggccg ttgtgggctt
      181 tgcggatgaa gtgctggagc atcttctcgg tgacttcgaa gttggcgata acgccgtctt
      241 tcatcggacg tatagcgacg atatttccgg gagttcggcc caacatgtct ttagcgtccc
      301 ggcccacggc ttcgacctgg ttggttactt tattgatcgc aacgatagaa ggttcagaaa
      361 cgacaatgcc gcgacccttg gcgtaaacga gagtatttgc cgtccccaga tcgattgcca
      421 ggtcgctgga aaacagactg aagatagatt tgaaagccat ttagacttat tcacttcccg
      481 tggttgtagt atggaacttc gggtgggatc ggcggcaaat atcagtaggg agagaatccc
      541 ttcgctcctt acctctttag acgcaccaaa cacgtccgac ctcctaagat acaccgttaa
      601 gacacgaacc gtaaactggt ttgttgtttg aacttgttat tttgagcggc tttgcctgaa
      661 aagtactcag cggatctcta tgggaaaaaa ctattctcgc gcaaagacgc aaagacgcaa
      721 agaaagccct taagaaacgc ggtagcgctt tgcgcctttg cgtctttgcg cgagagattt
      781 cttgcgatag aggcactttt cgggcaagcc tttttgagct ttgaactttg tactttgatc
      841 tttatggttt ctcagctcct cccggcacaa agcacaaaca caaagccctt tactaccggc
      901 cctacccctg caacacgctt cctgcgtcaa cctcgagcaa ctcgatgact tgtttgggcg
      961 gttcgtttgg ggccagatcc gcagcgaagc gtacacagtt cttgtttgcc gctttcgctt
     1021 catacatcgc cgtgtctgcg caagcaatca attgctgcgg agacgacgcg tgtttcggaa
     1081 atgccgccac accaaaactt gccgtcattg aaagctgtaa ccggcgcccg cccgtaaatt
     1141 gatgtctcag aattttttgc cgaatgcgct cggcgactgt tcccgcgagc tccgttcccg
     1201 tatcaggtaa aacgatgacg aactcgtctc cgccgtaacg cgcgaccgca tcggtatcgc
     1261 gtatcgaaga cagaattacg gcggccattt ccatcaacac gtgcgagccg acaagatgcc
     1321 cgtgctcgtc gttgatgcgt ttgaagtcgt cgagatccag aaatagtgcc gctacactgg
     1381 tgccgtaacg tcgcgcgcgg cggatttcgt tcagcagaaa ctggcgcagg tacctggcat
     1441 tatgcaattt cgttagatcg tctgtttgcg agagacgttc ggcttcggcg atgcgcaccg
     1501 cgttcgcgag cgcagaagcg atgggcagcg agagcgagtc gagcaaagcg acatcggtct
     1561 tcttaaaagc gcgcgctccg gcgccttgac gaatgccttc gatgacgccg agtattcgct
     1621 cgccgcagat cagcggcacg gcgagtacaa actgcccgcg ctcctttttt ttgatgattc
     1681 ccattccagc ggccgcacgc ttggctgctt tggtttcgga tccaagcggc agatcggatt
     1741 cacccgtgag cacgcgtcgc cattcttctt cgctcctgct gccactgcct tgttggcccg
     1801 gccggcgaac tgcaatcgct tccagcgtgg agctttcgat gcctttctct tcggagaaca
     1861 ggtagatggc ccaacggtcg gcgtcaatca attgctccag gccggcaagc gccgtgtttg
     1921 cagcaccact ctggtcgccg atgaaatgca gcgacgcaac cagatcaaac gcggctcgca
     1981 ggtcgttgaa tttgattttg ggcggcaacg aggaacagcc acgtccagcc ggtctttcga
     2041 cgacctcagg cagattcagt tcacctgaag ttccctggcc ttcgatctcg cgcaatagcg
     2101 ccggaagttg ggcggacttg tcggcgatcg ccagaaaacc gagccgcttc aaggtggcgc
     2161 catcgaggct gcgtagattc agactttggt acccgttcat ttgccgccgg caagcgacta
     2221 gcggcgcgcc cggccaaaag gctttcgcgt ggttgaccgc ggtgtgcagt tccaccatgt
     2281 cagcgcccgg caccacttcg taaagtacgg cgagcggcgg tggtccatcc aaatcggaga
     2341 gacctccgtg gctcttctgc tgccgcgcca acgccacctc aacttcaacc tcgcgcagac
     2401 cttcgatcag tgtcttgacg cccgccgcac gcaatgcctc gattaactcg taggccggcg
     2461 cttcgtgcgc atccgtcaat aggattacgt gactcgacac gttcgtagat ctccgactca
     2521 ggtccggcgc gtgttgcgac cggaaagtga aactctgtat tgagtgctgt tgccttgtgg
     2581 gtcctgcgcc tcgatgctca cttcgcgtgc gccacctgga gctgttacct ggatttggaa
     2641 cgagccatcg ggcgccacga tcgtttcgcg tcccgtaact ttgatcgttg tgcccggttc
     2701 ggcgcggccg cgaaccaggt acaaactgcc gccgagaagt tctgcagtga gatttgaaac
     2761 agcgacttgg gatcctgttc ctcgcgtggc gacaatgaac ttcagcggat cgctccagtc
     2821 gctcgcctga ccgctggcat ccgttgcgcg cacgcgccag aagtaaaccc caggccgcag
     2881 atcgctggcg ccaaattcgg tcgctgccaa ttgatcgcgt tcaataacct ttccgtcggc
     2941 cacgaagaac ggagaggtgg caacttcgac gcggtagtaa gcaggtgtgc ccgattgcgg
     3001 tcgttgccat ctcaacgcga cgctggcggc gccgtttgcg ccaacaaaaa ccttttcgag
     3061 atcgtcaggt gtggtgggct gtggtacgtc gagcagtttc tgggcggcgg acagtctgcc
     3121 cgagttgtta actgaaacgt attcgccgcc gcgtatagaa gccttttcac cggaccggtt
     3181 ggtggtttcg atcacacccg tcgagacgcg gatctcttca gttccttccg cgtttacgcc
     3241 gaagctggcg ttggtgcttt caccgacggt gtttttggtt ttcggtgttt cgacaacgtt
     3301 agtggtgcca tccgcctgtt gctccgtacg cactgacagt tgaccgctct caacgtgaac
     3361 gtgaacgttg gtgcgcttgc cgtcgtccgc gcgcgcgtta tcgcgcacga tgattgtgct
     3421 gttgggtttg acgacgagcg ttgagccgtc ggcgagaccg atacgggcgc ggccgctggc
     3481 ctgtgtctgt acggtgtcgc cgggataaag ctgggtatcg gcgttggcgg gaatgatctc
     3541 tcgcgtggcc gcccggatga cacggacgtc gccttcaaaa gagatgaacc tggccccggc
     3601 gggatagttc acctgggcca cgtttttgaa gggatttccg tatttccaga cgtagagggc
     3661 ggcaccggcg gcgacaccgc agaggagaaa gatggcgatc accagataga caaaacgctt
     3721 ctgaataatc caccaatcaa aagcaaagcg actggttcta tttgcgacca tttgctgagt
     3781 cgtggaggga gccgaaccaa ggttgagtcc aataaccctc ggtttttgtg tgcgattccg
     3841 gctatcgggc cgaggccgca cgtttatggg tgtgagtccg aacggtgtcg gcgggggaaa
     3901 tccgccgttt tccaggcagc aacactaccg gtcaggaatt taacacacga gactaagaac
     3961 gttcaagcac gtttaatctc gacttctgtt gggtcttcta cctcctcgcc tgggtccggg
     4021 ttggcgaagt ctgccggtaa atcgacttca attggcattt ccggcttgcc ggggtagatc
     4081 agcgagaaga caacggaacc cagcagcacg actgccacga acgccagtga gaacaggatc
     4141 gggatgtgga aaccgatggc gccgatcagc atttttatgc ctataaacgt cagcacgatc
     4201 gccaggccgg tacggagaaa acgaaacttc tcgactactc ccgcgagcag gaagtacatc
     4261 gatcgcagtc cgagaatcgc aaacacgttc gaggtgtaga cgatgaaggt gtcgcgcgtg
     4321 atggcgaaga ttgcgggaat ggagtccacg gcaaaaacga ggtccgtcac ttcgacgacc
     4381 atcaaaacga gcaacagtaa cgtgcccgtc cgtttgccgt tgactctggt aaagaacttt
     4441 tccccggagt aatgccgcga gatgggaaaa actcgcgtga ccgctcgcac ggcaatgtta
     4501 tgttcaggat tgatgtcgac ctcttcatgc ctgaacatct tgataccggt gtaaaccaga
     4561 aaagcgccga agatgtagat gatccaatgg aagcggttga tcagtgctgc gccgatgaag
     4621 atcatcgtca agcgcatgat cagagcgccc agcacgcccc aaaacagcac gcgatgttga
     4681 aactgcgccg gtactttgaa gtacgagaag atcagcagga agacaaagag gttatcgacc
     4741 gagagcgaca actcgatgag gtagcccgtg agaaactcca gaccccgaac cgagccctgg
     4801 taccgaaaca cgagacccgc aaagatcagg gccagcgtta cccaggcggt actccagatc
     4861 cacgcttctc tgtaacggat ggtgtgagct ttacgattga agagaccgag atccaggctc
     4921 aacattgtga cgataaagat gctgaatccc acccatagcc acgttgagcc gccgctaaat
     4981 atatccatgc agggtggtaa aatagcatct gaatttcgcc tgggcaaact gct
//
LOCUS       DASTEY010000619         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1052415, whole genome shotgun sequence.
ACCESSION   DASTEY010000619 DASTEY010000000
VERSION     DASTEY010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052415"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFO72_10795"
     CDS             <1..553
                     /locus_tag="VFO72_10795"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HET9479824.1"
                     /translation="QTLGTVVDRDLDTKQNVDRIAASLNKFETENLLRQDEGRMDLYE
                     ELNELIVKNGLKNTSGPTYTTLDPSGSKSTAGKNVVTKWQSFYPGIAVMVTVEGQYEA
                     VRRFIRDVERSKQFVVINEVEIQRATQNNAPASVEEGGASAAPGSSASGSGSRGSQVS
                     LQLNMATYFQRASATTGVSSDQE"
     gene            559..1476
                     /locus_tag="VFO72_10800"
     CDS             559..1476
                     /locus_tag="VFO72_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479825.1"
                     /translation="MSLVDLSKPGEKKKLIWAGALGLVAIIVLWWTFIGFDSGPKTAA
                     RPTATPTPQRAAQQNPQRPANGTVSPEVKNLEAFTEVQFNPASYNAPEAKRNIFAYYI
                     PPPKPVVTPTQPPPSPTPTPPVLLASVSPANVYARTAEFKLEAAGDKFTPAMKIFVDG
                     RELPTSYKSPQEVSATVPASFIAAPGARSVVVRTPDNSLFSNGATINVAAPPTPNYSY
                     IGIISPENRLADVALVQDKNNKNVLNVNKGDVLSGRFRVTSISEKELVLTDTTLKIKH
                     TIAMSEGDKGAAGPLSRPTPRVDAEDDEP"
     gene            1473..>2021
                     /locus_tag="VFO72_10805"
     CDS             1473..>2021
                     /locus_tag="VFO72_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479826.1"
                     /translation="MIASNGKVMNLGPARDHKICGSRRSGSAPLSDGISVARRRSEGG
                     YTLVALLAMMTVVALFAMAVAPSVRHQSQRELEKEAIFRGEQVADAIRAYYKYRSAIS
                     RGAGDQALPSSMDQLLQGIPIQGGSKNRQILRASAARDPLTIEGEWRFILPRTDALID
                     FQQSVMFYAGNIVPTTQDQQMAQ"
BASE COUNT          478 a          574 c          565 g          404 t
ORIGIN      
        1 gcaaacactc ggtaccgtcg tcgataggga cttagacact aagcagaatg tcgacaggat
       61 tgcggccagc ctcaataagt tcgagaccga gaacctgctg cgacaggatg aaggtcgcat
      121 ggatttgtat gaagagctca atgaactgat cgtaaagaac gggctcaaaa acacgtccgg
      181 tccaacttac acaacgctcg atccgtccgg gtcgaaatcg accgcgggaa aaaatgtggt
      241 aacgaagtgg cagagcttct atccgggcat cgcggtgatg gttaccgttg aaggtcaata
      301 cgaggccgtg cggcgtttca ttcgagatgt cgagcgaagc aaacagtttg tagtaatcaa
      361 cgaagtcgaa attcagcgcg cgacgcagaa caatgcgcct gcttcggttg aggaaggtgg
      421 tgccagcgcg gctccgggtt cctccgcgtc cggttccgga tcgcgcggct cccaagtgag
      481 tcttcaactt aatatggcga cgtacttcca gcgcgcgagc gccacgaccg gcgtctcctc
      541 tgatcaggaa taatagcgat gtcactggta gatctaagta aaccaggaga gaagaagaaa
      601 ctgatttggg ccggtgccct cgggctcgtg gctatcatcg tgctctggtg gacgttcatc
      661 gggtttgaca gcggtcccaa gaccgccgca cgacccaccg caactccgac tccgcaaaga
      721 gccgcacagc aaaacccgca acgccccgcc aacggcactg tttcgcccga agttaaaaac
      781 ttggaggcgt tcaccgaggt acagtttaac cctgcctctt ataatgctcc tgaagcgaag
      841 cgcaacatat tcgcgtacta cataccgcct ccaaagcccg tcgtcactcc gactcaacca
      901 cctccgagtc caacgccgac tccgccggtg ctgctggcgt cagtttctcc cgcgaacgtt
      961 tacgcgcgta ctgcggagtt caagcttgaa gctgcgggcg acaagtttac gccggcgatg
     1021 aagatctttg ttgacggtcg tgagctgccg acttcttaca agagtccgca agaagtttca
     1081 gcgacggttc ccgcgtcgtt cattgccgca ccgggagcgc gcagcgtcgt cgttcgcaca
     1141 ccggataaca gtctcttctc caacggtgcc accatcaacg tcgctgcacc cccgacgccg
     1201 aactacagct acattggcat catcagtccg gagaatcgcc tggctgacgt ggcattggtt
     1261 caggataaga acaataaaaa cgtactgaat gttaacaagg gcgatgttct aagcggcagg
     1321 tttcgggtga ccagcatctc tgagaaagag ttggtactga cggacaccac tttgaagatc
     1381 aaacacacga tcgcgatgag tgaaggcgac aaaggcgccg cgggtcctct gtcgcgcccg
     1441 acgccacgag tcgatgcaga agacgacgaa ccatgatcgc ttcgaatggc aaagttatga
     1501 atcttggacc agcacgcgac cacaagatat gcgggtcgcg tcgatcgggc tcagcgccgc
     1561 tctccgatgg catttcggtc gcccgcaggc gatccgaggg tggttacacg ctcgtggcgc
     1621 tgctcgcgat gatgaccgtc gtcgccttgt ttgcgatggc ggtagcgccg agtgtgcgcc
     1681 accaatcgca acgtgagctc gagaaggaag cgatctttcg cggcgaacaa gttgccgatg
     1741 cgattcgcgc ttattacaaa tatcgctcgg ctatatctcg cggcgcaggc gatcaagcat
     1801 tgccttcgtc gatggaccaa ctgctacagg gaataccgat tcaaggcgga tcgaagaacc
     1861 ggcaaattct tcgtgcatct gccgcgcgcg atccgctaac gatcgaaggc gaatggcgtt
     1921 tcatcctgcc acgcactgat gcgctgattg attttcagca gtcggtaatg ttctacgcgg
     1981 gcaacatcgt gcctaccact caggaccagc aaatggcgca g
//
LOCUS       DASTEY010000620         5025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_904315, whole genome shotgun sequence.
ACCESSION   DASTEY010000620 DASTEY010000000
VERSION     DASTEY010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904315"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFO72_10810"
     CDS             <1..528
                     /locus_tag="VFO72_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9479827.1"
                     /translation="PGMSGLELLKRVRDRGIQTPALFLTAHDALDDKVAGFEAGADDY
                     LAKPFSPRELEYRVEALLRRGGSVPQEQDGERIEVGDLVIDKRRHEVIRNGYRVDLTP
                     LEFQILELLASEPGRAWSRNALLDRVWSTDYEGYQRNIDPHINRLRKKLETDPKNPRY
                     VLTVRGVGYKLNEIP"
     gene            773..2338
                     /locus_tag="VFO72_10815"
     CDS             773..2338
                     /locus_tag="VFO72_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9479828.1"
                     /translation="MTSLSRRLLSLLVIGLLSLATVVAQQDVERKTVAITYPLDETVT
                     VKFRGTTLLPRLKGEAKVKRAGRRGTRVELSIDDLPRASELGGIYTTYVLWAISPDGH
                     VDNLGEIKRSGSSFIDSKLDVTTPLQSFALILTAEPHFLMKVPSRMVVMENLPPQRPN
                     GSQVETVDVRYIGNSSDYFRNVRVPEVADRDYRETPVSLLGARQAVSLAKYAGASEDA
                     PDELRLAEEQLQSAEQAWRLNQPLAEVDMEARRATSSGVHAEEVAAVRRAARKNREEI
                     RRRDDAVRAAEQNADALERQVAQLRTALEKEQRARELAERDAGSANDQLREKRVEIAR
                     LRDENQALRAESDAAKLKLARLEGEKQAEVARVEAERRAAERRIAEATLKETLAKYGT
                     VKETATGFRLVLPERIWTNARKATLATAAAAKLEPLAALLASNPDYQILIEAYTDNKG
                     NEVSLQQLTQERARVLSDRFQSAGVDPTRIQANGMGASNPLASNATVSGRTKNRRVEI
                     TFTSPLPKSTAAN"
     gene            2501..4543
                     /locus_tag="VFO72_10820"
     CDS             2501..4543
                     /locus_tag="VFO72_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479829.1"
                     /translation="MFPLDPLEILQVIGYSVGALLPLWMAVQLVLHRSKLSQLERLLA
                     ALAATMGGWHTSNLVITLHGLFDLGPDTWATLLRLADTIAVVSITFAYSFLLHVHLHL
                     WANAHGRDLNRSERVRVYLSYIPTIFLLVAVPRLWTGDYAPMLAKLSPFVIPFACWIA
                     YVLGLIALTELLIARKSGNRSEKRIMRTLAASFIGVGIVIFAALAFGLGEGTPAGVYL
                     KTIANLGSLLPSVLLAYYIYRYRYLEIIIEESLVVVTFATVVLTVYVFGIRTIGDWMT
                     QRYGVRPGLVEALLILALALLAAPLRGWLAQRFQRLFGREAALYKEIVSRIGAYAGQY
                     KDLSDLLHFVEQRTTEALGLRKLKIFTTDASDPWVKKALELSQARQWAPLDEDPLLLD
                     HGFKLAFPLRREEQVEGLLAVDAAAASLTTDVRSVIELLAGQVAIAIDDSRLFEENIR
                     LERELAERERLASLGRMAATVAHEIKNPLSAIKSIAQVMREDEQLTNEYERDLSLIVG
                     EADRLSQSVTQLLSFARKESAAEQPLGVDELLHSVIDLFRANAREHGIGLECVIESDA
                     TLTGKCVSAVRDAVSNLLLNALQATPTGGRVKLAASAANGELSITVEDTGAGVPADLR
                     ERIWEPFFTTRQRGTGLGLAIVRKRVQEVGGSASLASPRNGRGAVFHLRLPLNVGA"
     gene            4725..>5025
                     /locus_tag="VFO72_10825"
     CDS             4725..>5025
                     /locus_tag="VFO72_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479830.1"
                     /translation="MPEFLSDITPLAVFLIIAAIGFVFLLISLVVGDIFDSFGFETGL
                     DGSADGHTFMDSRVISVFVTAFGGFGAIGIQMGLSIVMSSLLGLGGGVVLGGLVSL"
BASE COUNT         1030 a         1400 c         1488 g         1107 t
ORIGIN      
        1 ccgggaatgt ccggcctcga gttgttgaag cgcgtccgcg atcgcggtat tcaaaccccg
       61 gcattgtttc tgacggcgca tgacgcgctc gacgacaagg tcgcgggatt cgaagccggc
      121 gctgacgact atctcgcgaa accgttcagt ccacgcgaac tcgagtatcg cgtcgaggca
      181 ttgttgcgtc gcggcggttc ggtgcctcag gagcaggacg gcgagcgaat cgaagtcggc
      241 gacctggtga tcgacaaacg gcgacacgag gtgattcgca acggctaccg cgtcgatcta
      301 acgccgctgg agtttcagat tctcgagctg ttggcgtcag agcctggccg ggcatggtcg
      361 cgcaatgcgt tactggatcg cgtttggagc accgactacg aaggctatca acgcaacatc
      421 gatccgcata tcaatcgttt gcgcaagaag cttgaaactg atcctaagaa cccacgctac
      481 gtgctgaccg tgcggggcgt tggttacaag ctcaacgaga ttccctgatg ctctggtggg
      541 tcggatcagg tttgctgctc gggtggctaa tcttattgat tgttcatcca gccggttgga
      601 ttcacttgtt gctgctgtcg ggaattactg tgcttgtggt ccagatcgca gcctaccgta
      661 agacaaaagc ctctcggaat attcgtggtt agtgtcttga cctgctcggt tccttcggca
      721 acaatgcgcc tgtttacggc atcaagagca ccaggagatt gctgccatga ccatgacttc
      781 tcttagtcga cgccttcttt ctctactcgt tatcgggttg ttgtccctcg ccaccgtcgt
      841 ggcgcaacag gatgttgaac gaaaaaccgt agccatcacc tatccattgg acgagacggt
      901 gacggtgaaa tttcgcggga ctacgcttct gccccgtttg aagggcgagg cgaaggtcaa
      961 acgcgccgga cgccgcggca cccgggttga actaagcatc gacgacctgc cgcgcgcctc
     1021 ggaactcggc ggcatttata cgacatacgt tctctgggca atctctcctg acgggcacgt
     1081 cgataacctc ggcgaaatca aacgcagcgg cagttcgttc atagactcga aactcgacgt
     1141 cacaacgccg ttgcaaagct tcgctctgat tctcaccgcc gagccgcact tcctgatgaa
     1201 ggtgccgagc cgcatggtgg tgatggaaaa cctgccgccg cagcggccga acggcagcca
     1261 ggtggaaact gtcgacgtcc gttacatcgg caactccagc gactacttcc gcaacgtgcg
     1321 ggtgccggaa gttgcggatc gcgattatcg cgagactccg gtgtcactgc tcggcgcgcg
     1381 acaagcggtg agtctggcga agtatgcggg tgcttccgaa gatgcgcccg atgaactgcg
     1441 tttggccgag gaacagttgc agagcgcgga gcaggcgtgg cgcttgaacc agccgttagc
     1501 ggaagttgat atggaagcga gacgggccac gtcgtcagga gtacacgccg aagaagttgc
     1561 ggccgttaga cgcgctgcgc gtaagaaccg cgaagagatc cggcgtcgtg acgatgcagt
     1621 gcgggccgcg gagcagaatg cggatgccct cgagcgtcaa gtggcccaac ttcgcacggc
     1681 tctggaaaaa gaacagcgtg cccgtgaact ggcagagcgt gacgcgggat cggcaaacga
     1741 tcagttgcgc gaaaagcgcg tggagatcgc acggttgcgt gatgagaacc aggcgttgcg
     1801 ggccgagagc gatgcagcca agctcaaact cgcgcgtctc gaaggagaga agcaggccga
     1861 ggttgcgcgc gtcgaagccg aacgccgcgc ggcggaacgg cgcattgcgg aagcaacctt
     1921 gaaagagacg ctcgccaaat atggcacggt caaggaaacc gcgaccggct ttcgtttagt
     1981 gcttccggag aggatctgga ccaatgcgcg taaggccaca ctggctaccg ctgctgccgc
     2041 gaaactcgaa ccactggcgg cgctgctggc cagcaatccc gactatcaga ttctgatcga
     2101 ggcctacacc gacaacaaag gtaacgaagt gtcgctgcaa cagctcacac aggagcgtgc
     2161 tcgtgtgttg tcagatcgat ttcaatcggc gggagtcgat cccacgcgga ttcaggcgaa
     2221 cggcatggga gcgtcaaatc cgttggcctc gaatgcaacg gtatccggcc gtacaaaaaa
     2281 ccgccgcgtt gagatcacat ttacgtcgcc gctgccaaag agtacggcgg cgaattaaat
     2341 atattgtcga ttttcgattg ccgattgccg attgccaaac tgtgctgcgg attgccgatt
     2401 gtcgattgcc aaattgtatt gtcgattgtt gatcgccgcc taactagtta ttcacggcca
     2461 cgatcaatcg gcaatcggca attggcaatc ggcaattctt atgttcccac tcgatccgct
     2521 cgaaatacta caggtcatcg gctacagcgt cggcgctctg ctgcccctgt ggatggccgt
     2581 ccagctagtc cttcaccgca gcaaactttc gcagctcgaa cgattgctcg ctgcgcttgc
     2641 tgcgacaatg ggcggctggc acacgagcaa cctcgtaatt actcttcacg gtttattcga
     2701 tctgggtcct gacacctggg ccacgttact gcgcctcgcc gacacgatcg ctgttgtcag
     2761 catcaccttc gcatactcct ttctgctcca cgtccacctg cacctttggg cgaacgcaca
     2821 tggccgcgat ctcaaccgaa gcgaacgcgt acgggtttac ctttcttaca ttccgacgat
     2881 tttcctgctg gtggcggtgc cgcgactctg gaccggcgat tacgcgccga tgctcgcgaa
     2941 gctgtcgccc ttcgtcatcc cgttcgcgtg ctggattgct tacgttctcg gccttattgc
     3001 cctgactgaa ttactcatcg cgcgaaagag cggcaatcgc agtgagaagc gaatcatgcg
     3061 cacgctcgcc gcttcgttca ttggtgtcgg catagtcatc tttgcagctc tcgctttcgg
     3121 cttgggtgaa ggaacgccgg caggtgttta tctgaagacg atagccaacc tcggctcgct
     3181 gctgccgtcg gtgttgctcg cttactacat ttaccgctac cgctatctcg agatcatcat
     3241 cgaagagagt ctggtcgtcg tgaccttcgc gacagtggta ctgacagtct acgtcttcgg
     3301 catacgcacc atcggcgact ggatgacgca acgatacggc gtgcggccgg gactagtcga
     3361 agctctgctc atcctggcgc tggcgctgct ggcggcgccg ctacgcggct ggctggccca
     3421 acgttttcaa agactgtttg gacgcgaagc tgcgctttac aaagagatcg tctcgcgcat
     3481 tggcgcttac gccggccagt acaaagacct ttctgacctg ctgcacttcg tcgaacaacg
     3541 aacgaccgag gcgcttggtt taagaaaact gaagatcttc accacagatg cttctgatcc
     3601 gtgggtaaaa aaagcgctcg aattgtcgca ggcaaggcaa tgggcaccgc tggatgagga
     3661 tccgttgttg ctggaccacg ggttcaagct cgctttcccg ttgcggcgag aagagcaggt
     3721 tgaaggtctg ctcgcggtcg atgcggctgc ggcaagctta actactgatg tacgctcggt
     3781 cattgaactg cttgcgggtc aggtcgcgat cgctattgac gactcgcgtc tgtttgaaga
     3841 aaacatccgg ttggaacggg aactcgccga acgcgagcgt ctcgcttcgc tgggccggat
     3901 ggccgctacc gtcgcgcatg agatcaagaa tcccttgtct gcgatcaaat cgatcgcaca
     3961 ggtgatgcgt gaagacgagc agctaaccaa cgaatacgaa cgcgacctca gtctcatcgt
     4021 gggcgaggca gatcgtctga gccagagcgt cactcagttg ttgagctttg cgcgaaagga
     4081 aagtgcggct gaacagccgt tgggcgtcga tgaacttttg cattcggtga tcgatctgtt
     4141 tcgggcaaac gcgcgcgagc acggcatcgg tttggagtgt gtgatcgaat cagacgcaac
     4201 gcttaccgga aagtgtgtct ccgcggtgcg cgatgccgtg tcgaatcttc tgctaaacgc
     4261 tctgcaagca acgccgactg gcggtcgggt gaaactcgcg gcgagtgcgg cgaacggcga
     4321 actttcaatt acggttgaag atacgggtgc gggcgtcccg gccgatcttc gcgaacgcat
     4381 ttgggaacca ttcttcacga ccagacagcg aggcacgggc ctcggactcg ctattgtccg
     4441 caagcgcgtg caggaagtcg gcgggtcggc ttcgctggcc tcgccgcgta acggccgtgg
     4501 cgcagtgttt catcttcgac tacctctgaa tgtcggtgcg tgatagtctt tacccaacgc
     4561 tcagtgtctc tgtgcctctg tggttgtttt ccaggaaata gttgaccaca gagacactga
     4621 gattgcacag agaagaggca aatctacttt tcgtgcaaag atgtccttca cgaataccga
     4681 ggccggttct cagggctcaa accacgactc gaaggagtaa ctcagtgcct gaatttctct
     4741 cagatatcac ccccctggcg gtttttctta tcatcgccgc aattggtttc gtgttccttc
     4801 tgatttcgct ggtggttggc gatattttcg acagcttcgg cttcgaaacc ggtcttgacg
     4861 ggtccgcgga cggtcacacg ttcatggaca gtcgggttat cagtgtattc gtaaccgcct
     4921 tcggagggtt cggcgccatc ggaatccaga tggggctttc gatcgttatg agttcgttgt
     4981 tgggactggg gggcggtgtc gtcctcggcg gtctcgtttc actgg
//
LOCUS       DASTEY010000621         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1019327, whole genome shotgun sequence.
ACCESSION   DASTEY010000621 DASTEY010000000
VERSION     DASTEY010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019327"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..691)
                     /locus_tag="VFO72_10830"
     CDS             complement(179..691)
                     /locus_tag="VFO72_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479831.1"
                     /translation="MSELDEAWALALAEAEHKARLAGRGDVADYLALRNSNDLLRKAG
                     VDWLMTTFVNLAGEANRAGAGIQLSNSEGHRFRIGTSTMVGRLVALSNGVRKLSVEAG
                     WPRVPRDGIVRGGGLAAANILHLGIRSAGEELLLSRSTTGTPHWTSITRRSREFHESD
                     IRRHLAILLQ"
     gene            789..>3732
                     /locus_tag="VFO72_10835"
     CDS             789..>3732
                     /locus_tag="VFO72_10835"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9479832.1"
                     /translation="MLPTYRVLNLGLAVLAAAGLFALTTVTPLAQSIQLPPPTSHISD
                     PGNVLDPQTRSRLEGLLANLKEKTTIELYVAMVDGTGGQDIAAFSQRLANDWKIGAKN
                     SRSKSLLMVVSAESKSSFTQFSRAAQSQLPDGVLGEMSYRMRGPLSEGRFTDAIAGGV
                     HLFVNALAEKIGFNAADLEPSTVAVEKTETASESPRTLLVSALEVDKTRPRIVAEATK
                     AQEPQPTPPIETPKTEPTPTETPAPEASPSESPDPKPAETTKTETTATEAPGTETPKT
                     EATEPAGRNLTAKGSTTVNKAPARKPTPPAEAEDDEETVSLTLVLPVTERVVKLKEWI
                     EIHPDSKERPRAIEYLVSAHATIGDKKLKDGDSLGGIEHLLRAIEEADPANTPDNLFS
                     GVISQIPTNLYLRGEREAAFKAAQNVETKFGSDPKRLLGLAGFYLSIERGDEVIRIAE
                     QAIKLEPDLAEAHRMLALGLHVSLRLDEAAAEYKRTLELDPASKASRITLADLYRASG
                     KTELALALYNEQLAAEPKDRAARAGKVISLLELSRQDEATAELDAALAEEPRNLALLT
                     GAAYWFAAHGNHDKAVDLARKAVAIESRYTWAQIALAHAYLGLQQPLNAERAMRYARQ
                     YGKFPTLNYELANVLASMGLYQEAADVLRESFEIRDDKVHTRLAGHLPASDASFIELL
                     APERLAGIFQLTSSDTPENAKLMKALLAFNAAITPQDGEKINEAMAAAAVAAAQEFAS
                     GTDNMRAFRQLYAASRLLRNGIGISVVIELVDAARKASDDALNTPVLTMAVQADEFRD
                     LRARALASGNVPDVAEAPRSVLGNILKGRIEDLHGWALFTQEKYPEAIEHLKQATEIL
                     PFNTPAWRSALWHLGAAYEQTDQKEQALDYYIKSYQGGDADAVRRLVIEHLYRQTHGS
                     LDGLEDRLSAIASEPPKAEPTAPVTTETTTSPATEPTASPAPETAPSPTPETTQPSQP
                     QPMSEESLR"
BASE COUNT          865 a         1135 c         1051 g          681 t
ORIGIN      
        1 gtcgcgccca ttcctgggac gatgggcgtc tttctcctgg gaccaagggc gttttttccc
       61 ggggacccag gggcgcgttt ttcctgggac cgcgggcgtc tcgcccgcac tggtcggtgg
      121 aggcgttctg cgcaatagtg cgcgcgagac gcccatcggt ctcaggaacg agacgccctt
      181 attggaggag gattgcgagg tggcgacgga tgtcggactc gtgaaactcg cgcgaccttc
      241 ttgttatcga ggtccagtgt ggtgtacccg tagtcgaccg cgacaacaac agttcttcac
      301 ccgctgaacg aatccctaag tgaagaatgt ttgccgctgc gagaccaccg ccgcgaacga
      361 ttccgtcgcg tggcacgcgc ggccagccgg cttcaacaga gagcttgcgc acaccgttgc
      421 tcagggcgac aaggcggccg accattgtgg aagttccaat acgaaaacga tggccctcac
      481 tatttgagag ttggatacca gcgcccgcgc ggttcgcttc acccgcgagg tttacgaagg
      541 tcgtcatcag ccaatcgacg cccgccttgc gcaagagatc gttcgaatta cgcagcgcca
      601 ggtagtcggc gacgtccccg cggccggcga gacgggcttt gtgctccgcc tccgcaagtg
      661 ctaacgccca ggcttcatcc aattcactca tgttctcagt cagacgagtt cggcaaaggc
      721 ataagaggcg aagagatggt atcatggccg ccgaatcgtc ccccaaaact caacacggaa
      781 caatcgacat gctcccaaca tatcgagtct tgaatctcgg cttagcagtt ctcgctgctg
      841 ctggcttatt cgccctgacg accgtcacgc cgcttgcgca gtcaatccag ttgccgccgc
      901 ccaccagtca tatcagcgac cccggaaacg tccttgatcc gcagacacgc agccggctcg
      961 agggcctgct cgcgaacctg aaggaaaaaa cgacgatcga actttacgtc gcgatggttg
     1021 acggcacagg cggacaagac atcgctgctt tttcacaacg cctcgccaac gactggaaga
     1081 tcggcgcgaa gaacagtcgc agcaaatctc tgctaatggt tgtttccgcg gaatcgaaaa
     1141 gttcgttcac gcagttcagc cgcgcagcgc agagtcagtt gcccgacggt gtcctgggcg
     1201 agatgagtta tcgcatgcgt ggtccgctga gcgaaggccg cttcacagac gctatcgctg
     1261 gtggcgtgca cctgtttgtt aatgcgctgg cagagaagat tggtttcaat gcggctgacc
     1321 tcgaaccgtc gactgtagca gttgaaaaaa ctgagacagc aagcgagtcg ccgcgaacgt
     1381 tgctggtcag cgcgcttgag gttgataaga cacgcccgcg cattgtggcg gaggcaacga
     1441 aagcgcagga gcctcaaccg actccgccca tcgaaactcc gaagacggaa ccaaccccaa
     1501 ccgaaacacc agcgcccgaa gcatcaccgt cagaaagtcc agatccaaaa cccgctgaaa
     1561 caaccaagac cgaaaccacg gcaactgagg cacccggcac agaaacaccg aaaactgaag
     1621 ccactgaacc ggccggcaga aatctcacag cgaaaggcag cactacagtc aacaaggctc
     1681 ctgctcgcaa gccaacgccc ccggccgagg cagaagatga cgaagagacg gtgtcattga
     1741 cactagtgct tccggttacc gaacgagtgg tgaagttgaa agagtggatc gagattcacc
     1801 ccgattcgaa agaacgcccg cgcgccatcg agtatctggt cagcgcacac gccaccatcg
     1861 gcgacaagaa gttgaaagac ggcgacagcc tgggcggtat cgaacacttg ctgcgcgcca
     1921 tcgaagaagc agacccggca aatacaccgg acaatctgtt ctcgggtgta atttcacaga
     1981 ttcctacgaa tttgtacttg cgcggcgagc gagaagcagc gttcaaagcg gcgcagaatg
     2041 tggagacgaa gtttggttcg gatcctaagc gactactggg cctggccggt ttttacttga
     2101 gtatcgaacg tggcgacgaa gtgatccgca ttgctgagca ggcgatcaaa ctcgagcccg
     2161 atctcgccga agcccatcgc atgctggcac tcggcctgca cgtctcactc cgtctcgacg
     2221 aagcggccgc cgagtacaaa cgaactctcg aactcgatcc ggcttcgaaa gcttcgcgca
     2281 tcaccttggc cgatctctat cgcgcgtcag gtaaaacaga actggcgctc gcgctttaca
     2341 acgagcagct cgccgccgag cccaaagacc gcgccgctcg cgccggcaaa gtaatttcgc
     2401 tactcgaact ctcgcgccag gatgaagcga ctgcggaact cgatgccgca ttggcagaag
     2461 aaccgcgcaa cctcgcgtta cttaccggcg ccgcctactg gtttgccgcg cacggcaatc
     2521 acgacaaggc tgtcgatctc gctcgcaaag ctgtggcgat cgagtcgcgt tacacgtggg
     2581 cccagatcgc gttggcccat gcgtatctcg gtttgcagca accactcaac gctgagcgcg
     2641 cgatgcgtta cgcgcggcag tacggaaagt ttccgacgct gaattacgaa ctcgcgaatg
     2701 tgctggcgtc gatggggctg tatcaggaag cggcggacgt tttgcgcgag tcgttcgaaa
     2761 taagagacga caaagttcat actcgcctgg ccggacatct accagcgagt gacgcaagtt
     2821 ttatcgaact gcttgcgccc gaacgcctgg ccggaatctt ccagctcacg tcatccgaca
     2881 ctccggaaaa cgcgaagctc atgaaggctc tgctcgcttt caacgctgca atcacgccgc
     2941 aggacggtga gaagatcaac gaggcgatgg cagcggcggc agtcgccgcg gcccaggagt
     3001 tcgcttccgg cactgacaac atgcgcgcgt ttcgccagtt gtatgccgcc agtcgcctgc
     3061 tccgaaacgg cattggtatt tccgtcgtga tcgagcttgt agacgcggcg cgaaaagctt
     3121 ctgacgacgc actcaacacg ccggttttga cgatggccgt gcaggcggat gagtttcgtg
     3181 acctgcgcgc acgggcactc gcttccggca acgttccgga cgttgctgaa gctccgcgaa
     3241 gtgttctggg caacattctg aaaggcagaa ttgaggatct tcacggttgg gcattattca
     3301 ctcaggagaa atatccggaa gcaatcgaac acctcaagca ggccactgag atccttccct
     3361 tcaacacacc cgcatggcgc agtgctctct ggcatctcgg cgccgcgtat gaacagaccg
     3421 atcaaaagga acaagccctc gattactaca tcaagagcta tcaaggtgga gatgccgacg
     3481 cggtccggcg gttagttatc gagcacctct atcgccagac tcacggttcg cttgatgggc
     3541 ttgaagatcg cctgagcgcg atcgcatcag aaccgccgaa agctgaacct acagcgccgg
     3601 tcacaactga aacgactact tctcccgcaa cggaacctac ggcttcgccg gcaccggaaa
     3661 cggcgccatc tccaaccccc gaaacgaccc aaccgtcgca accgcagccc atgtcagagg
     3721 aatcgctgcg ca
//
LOCUS       DASTEY010000622         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_986259, whole genome shotgun sequence.
ACCESSION   DASTEY010000622 DASTEY010000000
VERSION     DASTEY010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986259"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFO72_10840"
     CDS             complement(<1..1138)
                     /locus_tag="VFO72_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479833.1"
                     /translation="MRFKRLYPLFAIALSLMPLGATVNPGWSQSRRQPPTSNDKKNKR
                     PSETPKPGEEQQEPLPRDLEPTKPQDIEKVTISTQIVNVDAVVYHKKSRQIVTDLKKP
                     NFALFADGVQQTITNFSTPEAPITVAMVVEYSKWSEIFGLYGNRGYEPGTYEVIRPTA
                     MFLSQFIKPPDDYVSVIAFDIRPTPLTDFTNDPGRLQQVVTLLLRNQPAFRETNLFDA
                     LKFVLQGGRGDSVVLENSESEKADYAGLASVQGRRRAVILVASGIDTFSKINYGDARK
                     VLQNSGVPVYIIGTANMFKKLYGDSLPATDSLLGMPGRMSWLQADNTLQTFAKETGGA
                     YFPVTFEGELPKVLGSINALLRSQYSLAFNPGDVRDGKQHKLQVR"
     gene            complement(1231..1974)
                     /locus_tag="VFO72_10845"
     CDS             complement(1231..1974)
                     /locus_tag="VFO72_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479834.1"
                     /translation="MKKRFQLLLSTLLLVSLSTPLLADTIRLKDGSVIRGQVIGFKDQ
                     QFTILIGGSARGRRGQTTVYVEDVESIEFDSISCTSATEESALNNAPMSRPSSTPNNP
                     PENNVPDSRSNDSGPVVPTPTFFTIKVGVRADNANNGWTNSGLVVRKGQRLRISASGR
                     VSLGRGRFSLPGGSATIQDNDKLMRNENTGALIAVIGDDNDDFILIGPRRDFVAQRDG
                     VLFLGVNEGDLADNTGSYDIVIEAEASGR"
     gene            complement(2054..2875)
                     /locus_tag="VFO72_10850"
     CDS             complement(2054..2875)
                     /locus_tag="VFO72_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9479835.1"
                     /translation="MSELEVAKKRAATTYNAAADFYDHPANTFWERYGRRTVERLGLT
                     AGQRVLDVCCGSGASAIPAAKIVGPDGSVIGVDLAQNLLELARTKAQQQGLENVHFQS
                     GDMTRLSFEDNSFDVVVCVFGIFFVPDMESALRELKRVLRTEGRLAITTWGPRFFEPA
                     NTAFWNAVRNARPDLYKGFNPWDRICEVDDLLKLLEAAGLKELNVEAESGSQPVSSPD
                     DWWAMIMGSGYRGTLEQLDREAREEVRKQNLDFIQNAAVRSVEANVVYATAIKAA"
     gene            complement(2958..>3265)
                     /locus_tag="VFO72_10855"
     CDS             complement(2958..>3265)
                     /locus_tag="VFO72_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479836.1"
                     /translation="DDWDSLAAWSHMGYPTDLNSGQRPTWQGNFTVDGTDAAAQSILH
                     QADVFPGQSGGPVFGFWDGDVGPRAIAVQSWHTSSNNGASGSMDMRDLVVQARTEFA"
BASE COUNT          689 a          911 c          896 g          769 t
ORIGIN      
        1 ctctcacctg aagcttatgc tgcttgccgt cgcggacgtc gccgggattg aaagcaagac
       61 tgtactgact acgcagcaag gcattgatac tgccgagcac cttcggcaac tctccttcaa
      121 acgtcaccgg aaagtacgcg ccgccagttt ccttcgcgaa agtctgtagc gtgttgtcag
      181 cctgcaacca gctcattctt ccaggcattc ccaacaagct atccgtagcc ggcaacgaat
      241 cgccgtaaag cttcttgaac atgtttgcgg tgccgatgat gtataccgga acgccggagt
      301 tctgcagcac tttgcgcgcg tcgccgtaat tgatcttact gaacgtgtcg atacccgaag
      361 cgaccaggat cactgcacgc cgccgtccct gcacagacgc cagccctgca taatcagctt
      421 tctccgactc cgagttctcg agcactaccg agtcgcctcg accaccctga agcacaaact
      481 tcaacgcgtc aaacagattc gtctcgcgaa acgcaggctg gttccgcaac aacaaagtaa
      541 ccacctgttg aagccggccc ggatcgtttg tgaagtccgt caagggcgtc ggacgaatat
      601 cgaaagcaat tacggaaacg taatcgtcgg gtggcttaat gaactgcgac agaaacatcg
      661 ccgtggggcg aatcacttcg tacgtgccag gctcgtagcc tctatttccg taaagtccaa
      721 agatctccga ccacttgctg tactcgacga ccatcgccac cgtaatcggc gcttcgggcg
      781 ttgagaagtt tgtgatcgtt tgctgcacgc cgtcggcaaa aagcgcgaag ttgggtttct
      841 tcaggtcggt gacaatctga cggctctttt tgtgataaac aacggcatcg acgttgacga
      901 tctgcgtcga gattgtgact ttttcgatgt cctgcggctt ggttggttcc aggtcgcgcg
      961 gcagaggttc ctgctgttcc tcgccaggtt tgggagtttc gctgggacgt ttgttcttct
     1021 tatcgttcga agtgggtggc tgacgtctgg actgcgacca ccccggatta acagttgcgc
     1081 cgagcggcat taacgagagc gcaatagcaa aaagtgggta caaacgttta aatctcataa
     1141 tttcgactcc cgaggcgtaa agcggagggc aatccacaga ttacacagat taatttaatc
     1201 tgatgccggg gatccgacgt gagtttacca ctaacggccg ctggcttccg cttctattac
     1261 gatatcgtaa ctgccggtat tatcggccaa atcgccctcg ttgacaccca ggaacagcac
     1321 tccatcgcgc tgcgcgacaa aatctcgacg cggtccgatc aggataaagt cgtcgttatc
     1381 gtcgccaatt acggcaatca gcgcgcccgt attttcgttg cgcatcagct tgtcgttgtc
     1441 ctgaatagtg gccgatccac ccggcagcga gaaacgtcct cgtcccagtg aaacgcgacc
     1501 cgaagcactg atgcgaagtc gttgaccctt gcgcacgacc agcccgctgt tcgtccagcc
     1561 gttgtttgcg ttgtcggccc tgacgccgac tttgatcgta aagaaggttg gagttggaac
     1621 aacgggaccc gaatcgttcg agcgcgaatc cggaacgttg ttttcgggcg ggttattcgg
     1681 agtcgacgaa ggccggctca tgggcgcgtt gtttagtgcg ctttcttcgg tggcggaggt
     1741 gcaggagatc gaatcgaact cgatcgattc aacgtcctct acgtagacag tggtttgccc
     1801 gcgtctgcct cttgcgcttc caccgatcag gatagtgaac tgctgatcct tgaagccgat
     1861 cacctggcca cgaataacac tgccgtcttt gagacgaatg gtatcggcga gcagcggtgt
     1921 cgagagtgaa accaacaaca aagtggagag cagaagttga aatcttttct tcataatact
     1981 tctcccttat gggggcattc cgtgacgagg tttagcaaga ctcggatgct tttgcaagcg
     2041 ggagaggcgt tattcacgcg gctttgatgg cggttgcgta gaccacgttt gcttcgacgg
     2101 atcttactgc cgcgttttga atgaaatcaa ggttctgttt gcgcacttct tcgcgagctt
     2161 cgcgatcgag ttgttcgagt gtgccgcgat agccggagcc catgatcatg gcccaccaat
     2221 cgtccggtga gcttacgggt tgcgagccgc tttccgcttc cacgttcaac tctttgagac
     2281 cggcagcttc cagaagtttg agcaagtcgt ctacttcgca gatccggtcc cacggattga
     2341 agcctttgta gagatctggc cgcgcgtttc gaactgcgtt ccagaacgca gtgttggcag
     2401 gttcaaagaa acgcggtccc caggtcgtga tggcaagtct accttcggtg cgcaggacgc
     2461 gtttcaactc gcgcaatgcc gactccatat cgggaacgaa gaagattccg aacacgcaga
     2521 cgacgacgtc gaagctattg tcttcgaatg acaagcgcgt catgtcgccg gattggaagt
     2581 ggacgttctc gagaccttgt tgctgtgctt tggtgcgtgc gagttcgaga agattttgcg
     2641 cgagatcgac gccgatcacc gatccgtctg gtccaacaat ctttgccgcc ggaatcgccg
     2701 aagctccgct gccgcaacac acgtcgagca ctcgctgtcc ggcagttaat ccaagccgct
     2761 caacagtgcg ccgcccatat cgttcccaaa acgtgttggc tggatgatcg tagaagtccg
     2821 ccgccgcgtt gtaagtggtc gccgctctct ttttagcaac ttccaactcg ctcatcggcg
     2881 cgtaattgtg ccacaaaagt gacgcggata acgcagatta aatccggatt ttatctgcgt
     2941 tatccgcggc tggcttttta cgcgaactcg gttcgagcct gcactaccag gtcgcgcatg
     3001 tccatgctgc cgctggctcc gttgttggac gaggtatgcc acgactgcac cgcgatggct
     3061 cgtggtccaa cgtcgccgtc ccagaaacca aagacaggtc caccggattg tcccggaaat
     3121 acgtctgcct ggtgcaggat cgattgcgcg gcggcatcag tgccgtcgac tgtgaagtta
     3181 ccctgccagg ttgggcgttg gccgctgttc aggtcggtgg gataacccat gtgcgaccac
     3241 gctgcgaggc tgtcccagtc gtcgt
//
LOCUS       DASTEY010000623         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_458881, whole genome shotgun sequence.
ACCESSION   DASTEY010000623 DASTEY010000000
VERSION     DASTEY010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458881"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFO72_10860"
     CDS             <1..229
                     /locus_tag="VFO72_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9479837.1"
                     /translation="PDYVGLIGSKRRTSIVIDRLREAGASEDQLRKIRAPIGLDIGAV
                     SPEEVALAIIAEIVAERRGASGGSLSAWRRK"
     gene            345..1616
                     /locus_tag="VFO72_10865"
     CDS             345..1616
                     /locus_tag="VFO72_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9479838.1"
                     /translation="MPGEVVPVVTPRPTTTRRGGMFRALSHRDFRLFWIGAVLSNVGT
                     WMQAVAQGWLILLLTNSAFWLGLDAFVATAPGFLFTLAGGVFADLIDRRRLLIFTQIV
                     AGVAALGLAVLVATDLVNRWMVLGFSFVTGCCMALASPSYLAMTYDLVGREDLSNAIA
                     MNSTQFQLARVVGPALAGVAFKLFDLAGCFFANGLSFVAVVAALWVVRPERKTVATHS
                     VRDRRALWHDLTEGFRYVRNRPRVSALLLLSAVNSLFGAPYFTLVPIYARNIFRLGET
                     GLALMMGTAGAGAFFGALLVAYLGDFRRKGWFVLGGAITFGLFITAFAVSALLTMSLV
                     FLFGVGFSIVVSVAITNTLLQKLVTDQMRGRVMSMFILSFMGTMPIGNIVAGAVSNQF
                     GPQVTLATGGIIVTLVATGVSIFNKRLRDLH"
     gene            1851..>2077
                     /locus_tag="VFO72_10870"
     CDS             1851..>2077
                     /locus_tag="VFO72_10870"
                     /inference="COORDINATES: protein motif:HMM:NF025132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET9479839.1"
                     /translation="MNLPRALPVKSIKIDTKISSQVATTHVEQVFRNDTNFVLEGTYF
                     FPIPETASIAEFAIWDGDRRLVGEVRTREEAR"
BASE COUNT          354 a          566 c          631 g          526 t
ORIGIN      
        1 gcctgattat gttggactga ttggcagtaa acgacgcacc tcgatcgtga tcgaccggct
       61 tcgcgaggcg ggcgccagtg aagaccagct gcgaaagatt agagcgccga tcggactcga
      121 catcggcgct gtgagtcccg aggaagttgc gctggcaatc atcgcggaga tcgttgctga
      181 acgacgcggc gcgagcggcg gttcgctctc cgcgtggcga cgtaagtaaa tacagagtcg
      241 tggttcctgg ggagcgcagg cgtctcgcct gctttggccc gaagggcccg ccctgcgaac
      301 agtgcaggcg agacgcctgc ggtcccagga agaagtgaac aaagatgccc ggtgaagttg
      361 ttcctgtagt cactcccaga ccaacgacga cgcgccgtgg tggaatgttt cgcgcactct
      421 cgcatcgtga tttccgtctc ttctggatcg gagctgtcct ttcaaatgtc ggtacgtgga
      481 tgcaggcagt cgcccagggc tggcttatac tgctgttgac gaactccgct ttttggttgg
      541 gactcgacgc gtttgtggcc actgcgcccg gctttctgtt cacactcgcg ggtggcgtgt
      601 ttgcggattt gatcgatcgg cgtcggctcc tgatcttcac gcagatcgtc gcgggagtag
      661 ccgcgttggg actcgccgta cttgtagcta cagatttagt caatcgctgg atggtgttgg
      721 gcttttcgtt cgttacgggt tgttgcatgg cgctggcgag tccttcgtat ctggcgatga
      781 cttacgacct ggtgggtcgg gaagaccttt ccaatgcgat cgcgatgaac tcgacgcagt
      841 ttcaactcgc gcgcgtcgtt ggaccagcgc ttgcaggcgt ggcttttaag ttgttcgatc
      901 tggctggttg tttcttcgcg aacggtttgt cgtttgttgc cgtcgtcgcg gcgctctggg
      961 ttgtgcggcc ggagcgtaag acagttgcga cccactccgt tagagatcga cgtgctttgt
     1021 ggcacgattt gactgaaggg tttcgctatg tgcggaaccg gccgcgagtc tcggcgctgc
     1081 tgttattgtc agccgttaac agtctctttg gcgcgccgta cttcacgctg gtgccgattt
     1141 acgcgcgcaa catctttcgg ctcggcgaga ccggtttggc gttgatgatg ggaactgccg
     1201 gcgccggtgc gttctttggc gctttgttgg tggcttatct cggcgacttc cggcgcaaag
     1261 gctggttcgt gctcggcggc gcaatcacgt tcggtctctt tattacagcc tttgccgttt
     1321 cggcccttct cacaatgtct ttggtttttc tcttcggcgt tggtttttcg attgtagttt
     1381 cagttgcgat tactaacacg ctgctacaga aactggtcac ggatcagatg cgcggacgcg
     1441 tgatgagcat gtttatactc tcgtttatgg gcacgatgcc cattggaaac atcgtcgcgg
     1501 gcgcggtatc taatcaattc ggaccgcagg tcactcttgc caccggcggc atcatagtta
     1561 cgctcgtcgc gaccggagtg tcgattttta acaagcgcct ccgcgatcta cattgacaac
     1621 ttgcattaaa ctttggcgca agaagctccc cagcccaccg ttctagcacc gtccaagggg
     1681 taactttccg tgtggagatg agtatgccag tccgaaccag cctaattcgt gtcctcgtac
     1741 ttttgacaat ttgcctcgga ataacgtcca tcgcgagcgc tcagggcgtg atcatccccg
     1801 gcccgtgccc gcggccgcgc tgtcccgtgc cgccgattat cgagcggcct gtgaatctgc
     1861 cacgcgccct gccggtcaag tcgatcaaga tcgacacgaa gatcagttcg caggtggcta
     1921 ccacgcacgt cgaacaggtc tttcgcaacg acacgaattt cgtcctcgaa ggcacgtact
     1981 tctttccgat tccggaaact gcgtcgattg ccgagtttgc gatttgggat ggcgaccggc
     2041 ggctggttgg cgaggtcagg actcgtgaag aagcgcg
//
LOCUS       DASTEY010000624         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1448481, whole genome shotgun sequence.
ACCESSION   DASTEY010000624 DASTEY010000000
VERSION     DASTEY010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448481"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VFO72_10875"
     CDS             <1..254
                     /locus_tag="VFO72_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9479840.1"
                     /translation="SMIKRHAERAGIKNVSPHTLRHSFATHLLQHGADSRSVQALLGH
                     SDISTTQIYTHITDVNLRSAYDRHHPRARVKSKHDEPVN"
     gene            254..850
                     /locus_tag="VFO72_10880"
     CDS             254..850
                     /locus_tag="VFO72_10880"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HET9479841.1"
                     /translation="MFTLREPSDTDIERFLYSQSNLHFSYGEVGATRPNPALEIAAPP
                     GYTLDHNRVQLGRGTEIFQNGVNALKQWRQFELGWVTIAPRGVKLERDATVAVKAWAY
                     GMWSLNACRVVYVIDEQEPLRRFGFAYGTLPDHVARGEERFLIEWDARDERVWYDILA
                     FSRPRHPLVRAGFPVARMLQKRFARDSLRVMKSLVNSV"
     gene            complement(812..2338)
                     /locus_tag="VFO72_10885"
     CDS             complement(812..2338)
                     /locus_tag="VFO72_10885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET9479842.1"
                     /translation="MNKFDVIVIGAGINGAGVARDAAMRGLKVLLIDKGDVGGGTSAG
                     STRLIHGGLRYLEHFEFGLVRESLKERETLLKIAPHLVRPLLITIPIYNESQRGRATI
                     RAGMIAYDLLSLGKSLPRHRMHSSAETLTQMPGLKAEGLLGSAVYFDAQVEFAERLVL
                     ENVLAAIEHGATVLTYTPAKKLLCEERSVRGVALDGDNRDTALADVVINAAGPWVDRL
                     LDLNGEENERLIGGTKGSHTVVAAFAGAPCSAVYLEAVSDRRPFFVIPWNGNYLIGTT
                     DVRFEGDLDRVRAEDWEVDYLLAETNRAFPAAKLTRESVLYTYSGVRPLPFTRNEDEQ
                     RTTRRHFIREHPRFKNLISIVGGKLTTYRSLAEECVDLVFQKLGRKAPPCKTAQFPLP
                     GGVGDPPENVCERWCRVYGFRAREIAEFARRLDDQNPLTAELIFSFKHELARTLGDCF
                     LRRTMIGLNADLGLGEIRAAAEIGRRWLGWSEERAVREVESYREEISDRIDEGFHHSQ
                     "
     gene            2571..>3632
                     /locus_tag="VFO72_10890"
     CDS             2571..>3632
                     /locus_tag="VFO72_10890"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET9479843.1"
                     /translation="MQVSRLNASLLVLLLTLFSITITARAADDDDEPDDYDVKARVVR
                     ISLITGEVSLKRNGNQDWERARLNYPLVEGDTVSTDKESRMEIQVDARNFVRLAPGSA
                     LRIVTLRDEGVALSLLEGTTVVRLVKFDRSREYFEVDAPRATMTAEKSGLYRVDVPRD
                     GRVRLTVRDGGSARIYSDTSGFSLRDGRSAELVVTGENAGDWDFLAAASRDAIDDWVN
                     DRERYLAQRLRYDVEYYDEHVWGAEELAAYGSWTNTSDYGWIWRPHPATISAYADWAP
                     YRHGHWVWCPPYGWTWVGYEPWGWAPYHYGRWVFHNNAWAWVPRTKFNRKRSWWRPAL
                     VAFVSLNFSFGNDICWYPLS"
BASE COUNT          806 a         1084 c          933 g          809 t
ORIGIN      
        1 ggtcgatgat caaacgtcat gcggaacgag cggggataaa gaacgtttca ccacacacgt
       61 tacgtcacag ctttgcgact catctgctgc aacacggggc tgactcgcgc tccgttcaag
      121 cactgctggg ccatagcgac atatccacaa ctcagatcta cacacacatc actgacgtga
      181 acttgcgctc ggcttacgat cggcatcatc ctcgcgcgcg cgtgaaatca aagcacgatg
      241 agcctgtcaa ctgatgttca cgctgcgcga accttcagac acggacatcg aacgctttct
      301 ttacagtcaa tcaaacttac actttagtta cggcgaagtc ggcgccactc gccccaatcc
      361 tgccttggaa atcgccgcgc ctcccggtta cacgctcgat cacaatcgtg tccaactcgg
      421 ccgcggcaca gagatctttc aaaacggagt gaacgcattg aaacagtggc ggcaattcga
      481 actcggctgg gtcacgatcg ctcctcgtgg tgtgaagctt gaaagggatg cgacggtggc
      541 ggtcaaagcc tgggcgtatg gcatgtggtc attaaatgca tgtcgcgttg tttacgttat
      601 cgatgaacag gaaccgttga ggcggtttgg ttttgcgtac ggcaccttgc cggatcacgt
      661 cgcgcgtggc gaagaacgtt ttctgatcga gtgggatgcg cgtgacgaga gggtgtggta
      721 cgacattctc gctttttcaa ggccgcgaca tccgttagtg cgggccgggt ttccggttgc
      781 gcggatgctg cagaagcgat tcgcgcgaga ttcattgcga gtgatgaaat ccctcgtcaa
      841 ttctgtctga aatctcttct ctgtaactct caacttcgcg taccgcacgc tcttcactcc
      901 aacccagcca acgcctgcca atctccgcag ccgctcgaat ctcaccgagc cccagatccg
      961 cattcaaacc aatcattgtg cgtcgcagaa aacagtcgcc caaagtcctg gccagctcat
     1021 gtttaaaaga gaagatgagt tcagccgtca gcgggttctg atcgtccaac cgccgtgcaa
     1081 actctgcgat ttcccttgct cgaaagccat aaactcgaca ccagcgttca cacacgtttt
     1141 ccggaggatc tcccacacct ccaggcaatg gaaactgcgc cgttttacac ggaggagctt
     1201 tcctgcccag tttttgaaac accagatcga cgcactcttc ggcgagactc cgatacgtag
     1261 tcaatttacc accgactatt gaaatcagat tcttaaaccg cggatgctcg cggatgaagt
     1321 gccggcgcgt ggtgcgttgc tcgtcctcgt tccgggtgaa cggcaacggc cgtacgcccg
     1381 aatacgtgta gagcacactt tctctcgtca atttagccgc cggaaacgcg cgattcgttt
     1441 ccgccaacag gtagtcgact tcccaatctt cggcgcgaac ccgatccaga tctccttcaa
     1501 agcgaacgtc cgtggttcca attagatagt ttccgttcca cggaataacg aagaacggac
     1561 gccgatcact aacggcctcc agataaacag cgctgcacgg tgcccctgca aacgccgcca
     1621 ccacggtatg actacctttc gttccaccaa ttagccgctc attctcttca ccgttcaaat
     1681 ccaacagccg atctacccac ggcccagcag cgttgatcac aacgtccgct agcgccgtat
     1741 ccctgttatc cccatccaac gcgaccccac gaacgcttct ctcttcgcat aacagcttct
     1801 tcgctggcgt gtaagtcaaa acggtcgcgc cgtgttcaat cgcagccaac acattctcca
     1861 gcaccaatcg ctcagcaaac tcaacctgcg catcgaaata aaccgccgac cccagcaagc
     1921 cctcagcctt caaccccggc atctgtgtaa gagtctctgc agacgagtgc attcgatgac
     1981 gcggcaacga cttgcccaac gagagcaggt cgtacgcaat cattcccgcg cgaatcgtcg
     2041 cccggccccg ctgactctca ttgtaaatcg gaatagtaat caataacggc cgaacaagat
     2101 gtggcgcgat tttgagtaga gtctcacgct ccttcagcga ctcccgcacc aacccaaact
     2161 caaagtgttc gagatatctc aatcctccat ggatcagtcg cgtcgatccg gcactagtcc
     2221 ctccgcccac gtctcctttg tcgatcaaca acactttcaa cccgcgcatc gccgcgtcgc
     2281 gcgcgacacc tgcgccgttg atccccgctc caataacgat tacgtcaaat ttgttcatac
     2341 gatttcgtat cggctacaca ccgtctacga catcatttgc gcatcacgtt taatcacgca
     2401 ttcgaacagt ccgcttgaga cacctcaaat ttacagatca cactcgaagc ttgtcgcttg
     2461 agacatctca attgtcaaaa ctgccgcact cctgctttga tcagggcata cactttgccg
     2521 cgttctcaca cgccaactgg atcaaaccaa ccaaaccaag gagcaggacc atgcaagtct
     2581 cccgactgaa cgccagcctt ctagttttac tactcacgct tttttccatc accatcactg
     2641 cccgcgccgc tgacgatgac gacgagcccg acgattacga cgtaaaagct cgggtagtcc
     2701 gcatcagttt gatcaccggg gaggtaagtc tcaagcggaa cggcaaccag gactgggaac
     2761 gcgctcggct gaactaccct ctcgtcgaag gcgacaccgt gtccacagac aaagaaagcc
     2821 gaatggagat acaggtcgat gcgcgcaact ttgtgcggct ggcgcccggt tcggcacttc
     2881 gcattgtgac tctgcgcgac gagggcgtcg cgcttagttt gcttgaagga acgactgtgg
     2941 ttcgcctcgt taagtttgat cgcagtcgtg aatattttga agtcgacgcg cccagggcca
     3001 caatgactgc cgagaagtcc ggtctttacc gcgtcgacgt gccgcgcgac ggacgcgtgc
     3061 gtttgactgt gcgcgatggt ggcagtgcgc gaatctattc cgacacctcc ggcttttcgc
     3121 tgcgtgatgg ccggtcggcg gagttggtcg taacgggcga gaacgcaggg gactgggatt
     3181 ttctggctgc tgcgtcacgc gatgcgatcg atgattgggt gaacgatcgc gagcgttatc
     3241 tcgcccagcg actccgctat gacgtcgaat attacgatga gcacgtgtgg ggtgctgaag
     3301 aacttgccgc ttatggttcg tggacgaata cgtcggacta cggatggatt tggcggccac
     3361 atccggcgac gatcagtgcc tacgcggatt gggcgccata tcggcatggg cattgggttt
     3421 ggtgcccgcc gtatggatgg acgtgggttg gttacgagcc gtggggttgg gcgccgtatc
     3481 actacgggcg ttgggtgttt cacaacaacg cgtgggcgtg ggtgccgcgc acaaagttca
     3541 atcggaaacg aagttggtgg cggccagcgc tggttgcgtt tgtgtcgctg aacttttcgt
     3601 ttggcaacga catttgctgg tatccgttgt cg
//
LOCUS       DASTEY010000625         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_195556, whole genome shotgun sequence.
ACCESSION   DASTEY010000625 DASTEY010000000
VERSION     DASTEY010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195556"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1169
                     /locus_tag="VFO72_10895"
     CDS             144..1169
                     /locus_tag="VFO72_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9479844.1"
                     /translation="MKADIDLDVGGKTFASGLLPELITVLRRSRPGDLVAVIGDEESI
                     GPELERWCRFTGNALLEATSENGRSRWVFRCGTVAPEENNRPLGSRLWLYTNFDCNLR
                     CNYCCVRSSPTVPRRELGLDRVKRIAREAADLGVKEIFVTGGEPFLLEDIQEILIACA
                     AVAPTTVLTNGMLFTGRRAESLRALPRDRIVLQISLDSATPDRHDLHRGPGTWARTRA
                     GIAYAVAQGFRVRLAATVSTDAEAEEFRQFLDKEKIAEEDRVIRRIALRGAASEGVAV
                     SRTDLVPEVTITADGVYWHPVGAEDEDLLVTREIFPLSESFAAVRGAFDREHEHSNKL
                     ARIFTCA"
     gene            complement(1156..1995)
                     /locus_tag="VFO72_10900"
     CDS             complement(1156..1995)
                     /locus_tag="VFO72_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HET9479845.1"
                     /translation="MNKTIWLGAVAYDPKVVTIWEGMRRYFHEEAQLSVEIVLFQSYE
                     AQVLALLAQRGEAIPRIDIAWNTNLAYLQADEWSGHACRAIAMRDTDLGWMTKIVAVN
                     GGPISSVADLKDRTLALGSRDSGHAAILPVYFLEQQGMREGIDYQTLRFNSDVGKHGD
                     TGNSEVEVVRAVLDGRADAGAIGSPFWNTVRNERLVPEDALREIWSSPTYNHCMFTAR
                     SDLDVEREHQFVEALSAMSYDNPVHRAILDAEGLQRWLPPHLDGYDSLRQAAFEQGFF
                     THR"
     gene            complement(2091..3251)
                     /locus_tag="VFO72_10905"
     CDS             complement(2091..3251)
                     /locus_tag="VFO72_10905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9479846.1"
                     /translation="MLLMTDDAVILKTREIASSVLAPSAGQNDREGRFSTEAVQSLGE
                     SGLLGLLLPVDLGGAGLGPRTFADVTAILAEADGSVAMVYVMHICAAATISAARESAA
                     QAVTPVLQEIAAGRHLSTLAFSEAGSRSHFWAPISRAQRNGDGVHISAKKSWVTSAGH
                     AQSYVVSARAPEGEGPTDSTLYLVAAGTRGLSVAGAWDGLGLRANASAPIVLDDCQVS
                     LDFQLTDDGDGFPVMLNVVLPLFNLGTAAVALGLCRAAVTGTASHLNNAKFEHLGQSL
                     GESLPTLRAQLATMQIETDGLSTRIDDLVDHLEKPRETTMLRVLETKAAAGDAAISVT
                     STAMRICGGAAFSKHLSIERLFRDAHAGAVMAPTGDVLREFIGKSLLGIPLF"
     gene            3278..>3615
                     /locus_tag="VFO72_10910"
     CDS             3278..>3615
                     /locus_tag="VFO72_10910"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HET9479847.1"
                     /translation="MMLTPFCALGLMAKAPFAGEVKTRLVPPLTAREAAELNICFLRD
                     MAANIDSVGETAATSGLIVYTPTGSESAFDGVAPGGFKLLAQRGASLGERLRNATDDL
                     LRQGYGAVCL"
BASE COUNT          788 a         1008 c         1039 g          780 t
ORIGIN      
        1 gaaagctggg ccgctccttg tctcgacgga ccacaagggt acggcagacg gaaacacggt
       61 gcgggttttc atttcggatg tcgccgttag aatgacggga tcacaaaact ggatcgaggc
      121 gcagtgattc agacaccaat ccgatgaagg ctgacattga tctggacgtt ggcggaaaga
      181 ctttcgcatc tggtcttttg ccggagctga ttactgtgct acgtcgcagc cggcctggcg
      241 atctggtcgc agtgattggt gacgaagaaa gcattggacc ggagttggaa aggtggtgcc
      301 gattcaccgg aaatgcactg ctggaggcta cgagcgaaaa cgggcgttca cggtgggttt
      361 tccgttgtgg aactgttgcg cctgaagaaa ataatcggcc gttgggctcg cggctttggc
      421 tctatacgaa cttcgactgt aatctgcgtt gcaactattg ttgcgtgcgt tcctcaccca
      481 ccgtgcctcg ccgagaactg ggactcgacc gtgtaaaacg catagctcgc gaagcggcgg
      541 accttggtgt gaaggagatc ttcgtaaccg gaggcgagcc gtttctgttg gaagatatcc
      601 aggagattct gattgcttgc gctgccgtcg ctccgaccac ggtgctgacc aacggaatgt
      661 tattcaccgg gcggcgcgcg gaaagcttgc gagcgttacc gcgcgatcga attgtgcttc
      721 aaatcagctt ggacagtgcg acacctgaca ggcacgacct ccatcgcgga cccggaactt
      781 gggcgcgaac gcgagcaggt attgcttacg ctgtggcgca ggggtttcga gtgcgactgg
      841 cagcgactgt ttctaccgat gccgaggccg aggagttccg tcaatttctc gataaggaga
      901 agattgcgga agaggaccga gtcatacgcc ggatcgcatt gcgtggtgct gccagtgaag
      961 gggttgccgt tagtcgaact gatttggtgc cggaagtcac gataactgct gacggtgtgt
     1021 actggcatcc ggtcggggcg gaagatgaag atcttctcgt cacgcgcgaa atctttcctc
     1081 tgtcagagtc tttcgctgcg gttcgcggcg cctttgatcg tgaacacgaa cactcgaata
     1141 agctggcaag aatctttacc tgtgcgtgaa gaaaccctgt tcgaacgcag cttgccgcaa
     1201 agagtcgtaa ccgtcgaggt gaggtggtag ccaacgctgc aaaccttcgg cgtcgagaat
     1261 cgcgcgatgg accgggttgt cgtagctcat ggctgagagc gcttcgacaa actgatgctc
     1321 acgttcgacg tccagatcgg accttgctgt gaacatgcaa tggttgtagg tgggggagga
     1381 ccatatctca cgtaatgcgt cttcgggcac gagacgttcg ttgcgcacag tgttccaaaa
     1441 aggactgcct atggcgccgg cgtccgctcg cccatccagg acggcgcgca caacttccac
     1501 ttcgctgttc ccggtgtcgc catgtttgcc gacatcgctg ttgaagcgca gcgtctggta
     1561 gtctattccc tcgcgcatgc cttgctgttc caaaaagtag accggaagaa ttgctgcgtg
     1621 tccgctatct cggctaccaa gcgcgagcgt tcgatctttt aaatccgcga ccgaagaaat
     1681 tggaccaccg ttgacagcaa caatcttagt catccaaccg agatcggtgt cgcgcatggc
     1741 gatcgcacga caagcatggc cactccactc atcggcctgc aagtaggcaa gatttgtgtt
     1801 ccaggcaatg tcgattcgcg gaatcgcctc cccccgttgg gccaggagtg cgagcacctg
     1861 cgcctcgtag ctctggaaca gtacgatctc aacggaaagc tgcgcctcct cgtgaaaata
     1921 gcgccgcata ccctcccaaa tcgttacgac ttttggatcg taggcaacgg ctcccagcca
     1981 gattgttttg ttcaaaagct ttgcctccac cgagagaagg atttctcacg caaaaacagc
     2041 gctttagata cgcggccgcg gcgttgcgtc tttgcgcgag aaatcctctc tcaaaagagc
     2101 ggaattccca acaatgactt accgataaac tcgcgcagaa catcgccggt tggcgccata
     2161 acggcaccgg cgtgcgcatc gcggaacagt cgttcaatac tcaaatgctt tgagaacgcc
     2221 gcgccgccgc aaatgcgcat tgcggttgag gtaacgctga tagcggcatc gcccgcggct
     2281 gccttcgtct cgagcacacg cagcatcgtg gtctcgcgcg gcttttcaag atgatcgacc
     2341 agatcatcaa tccgtgttga cagcccgtcg gtctcgatct gcatcgtcgc gagttgcgcg
     2401 cgaagcgtag gcagactctc accgaggctt tggccaagat gctcgaactt cgcgttgttg
     2461 aggtgcgatg cagttcccgt gacggccgct cggcataatc ccagggcgac tgctgccgta
     2521 cccaaattaa acagcggtag caccacgttc agcattaccg gaaagccgtc gccgtcatca
     2581 gtaagttgaa aatcgagtga cacttgacag tcatccagca cgatcggtgc agaggcgttg
     2641 gcgcgaagtc ccaaaccgtc ccacgcgccc gctactgaaa ggccgcgtgt tcctgctgcc
     2701 acaagataga gggtcgaatc ggtaggtccc tccccttcag gagcacgcgc agacacgaca
     2761 tagctttgtg catgacccgc gctggtaacc cacgatttct ttgcactaat gtggacgccg
     2821 tcaccatttc gttgagcacg cgagatggga gcccaaaaat gactccgtga gccggcctcg
     2881 ctgaaagcga gcgtcgataa gtggcgaccc gcagcaatct cctgcagtac cggagtaact
     2941 gcctgagctg cgctttcgcg ggctgccgag atggtcgcgg cggcgcatat atgcatcacg
     3001 taaaccatcg cgacagaacc gtctgcctca gcgaggattg cggttacatc agcgaacgtt
     3061 cgtggaccta atcctgcacc accaagatct accggcagca acaggcctag caatccagac
     3121 tcaccaaggg attgaactgc ctcagtagaa aatcggccct ccctgtcgtt ttgcccagcg
     3181 gaaggtgcca gcacgctact cgcgatctcc cgagttttca atataactgc atcgtcagtc
     3241 atgagcagca tgttatatca tgcgatgaca aaactcgatg atgcttacac ccttttgcgc
     3301 gctcggactc atggccaagg cgccgtttgc gggcgaggtt aaaacaagac tcgtgccgcc
     3361 tttgacggcg cgagaagccg cggaactcaa tatctgtttt ctgcgcgaca tggccgcgaa
     3421 catcgatagc gttggtgaga cagcggcaac gtcaggcttg atcgtctata cgccaaccgg
     3481 ttccgagtct gcgtttgatg gtgtcgcgcc tggaggattc aagttactgg cacagcgcgg
     3541 tgcatccctg ggcgaacggc tccgcaatgc cactgacgat cttttaagac aggggtatgg
     3601 cgccgtctgt ctcat
//
LOCUS       DASTEY010000626         4905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_838664, whole genome shotgun sequence.
ACCESSION   DASTEY010000626 DASTEY010000000
VERSION     DASTEY010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838664"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VFO72_10915"
     CDS             complement(<1..1536)
                     /locus_tag="VFO72_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479848.1"
                     /translation="MSEFFESFASLFRKPARDTDEKPFHAEFYSVERLEQYAQALAAE
                     HKTVTRKGRAILLPRLAENGRILESAYKALVEALREGRAISPAAEWLVDNYHIVEEQL
                     REIRHDLPKSYYHELPKLTDGELAGYPRIYAVALELITHTDSRLDTNSLRRFIAAYQT
                     VSPLSIGELWAVAITLRLALVENLRRLAVNIARARAEREEADLLADKLLELASLQPTA
                     INTTVTERLGKREELPQTFLVQLIQRLREQHPSVMPVMDWIEKQLGRQGASVEQIIHT
                     EHQRQAAAQVTVGNIITSMRLLSTLDWNDFFEKVSLIEPLLGKDPAGAYLRMEFASRD
                     RYRHVIERISKRTRTSELKIAQATVKLATEAKENDAQAKKHVGYYLIDEGLPQLEAIF
                     RYRPRTREKLRRFLVRHATASYLGVIALLTILLLGFLLTAMSLAGASWLVLVAAGLLA
                     LIPASDLAVTVVNWDLTHFFPPRLLPRIETLTGIPENTSTFVVVPTIFFSESQVQELI
                     ERLE"
     gene            1747..2244
                     /gene="ruvC"
                     /locus_tag="VFO72_10920"
     CDS             1747..2244
                     /gene="ruvC"
                     /locus_tag="VFO72_10920"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9479849.1"
                     /translation="MRVLGIDPGSQTMGWGVLEGDGRRYSLVAYGSVRAPASLKFSAR
                     LLRMCNGLEEVIAEHRPDACALEDAFLATNVKVSMKLGQVRGVVLLVAERAALEIHEY
                     SPRLVKQSVVGYGNAEKHQVQEMVRLLLSLKSVPSPHDAADALAVAICHFHHAPFKQR
                     LAVLV"
     gene            2332..3054
                     /locus_tag="VFO72_10925"
     CDS             2332..3054
                     /locus_tag="VFO72_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479850.1"
                     /translation="MNPHKLTIRLLLSLVVLYLCAPAAAAQPRKPPAGGRLAVVVDER
                     LAALRGTPQLCGRLVRRLSRGRMIAIRGSKTTAEGVVFFLVNVTRRTRGWIQREAVVA
                     VGRAGEDRRLLELINASYGFDRTARARIFLDYFPRSPLRPRVLLLLGNAAEEQAAELS
                     REAARRLNATMGSAPEFSYFLNYSGLDRYNRQRVGFVFDRATRRFHYDGAAWRELVRR
                     YPNSAEAAEARQHLDELAAISQ"
     gene            3128..3694
                     /locus_tag="VFO72_10930"
     CDS             3128..3694
                     /locus_tag="VFO72_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9479851.1"
                     /translation="MQAALKPLRLVKNEPAGELETLFQTHHGRVFRTAQRITGSAADA
                     EDVLQNVFLRLVKGQETYDLSRNPEAYLSRAAINASLDLLRSRSRARSVGLDDADADT
                     LAGGARSPESLHADRELQSLVRQAVSRLGKTAGEMFVLRYYEGLGNKEIAELLETSPF
                     VVGVVLHRARTKLRKEIGHYLEKHHEKE"
     gene            3681..>4905
                     /locus_tag="VFO72_10935"
     CDS             3681..>4905
                     /locus_tag="VFO72_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9479852.1"
                     /translation="MKRNSEEILDKVTAEIRHENVDPAVVSAAADRVWARVSVAAGET
                     EVQMPAVDRIEGCADFQSLIPAYLAGKLSEARSMLLVDHTHECIPCRRAMKEARSRKT
                     VVAKAPAKPSRYSLQPVVMRWGIAAALVIGFGLLAVPFVQRYWPFGEFEATVQAAEGQ
                     VYQVADTRTAAVSTGAKLQKGEKVRTAKDAHAVVRLSDGSLIEMKERSELYLTKTGQG
                     TTIHLDRGSIVVEAAKQNDGKFFVESGDSLVSVTGTVFSVNNCTKGSRVSVIEGEVNL
                     NHAGSDRVLRPGEQATTSPSIATIPIKDEVAWSKNASRYASLLSGLATLKNELQTVSQ
                     PGVRNSTHLLDLMPENTVVYAALPNLTNTITESHRIIQERMSQNAALRAWWEKEQSGR
                     AQNMDQVVQTIREFGS"
BASE COUNT         1031 a         1274 c         1514 g         1086 t
ORIGIN      
        1 ctcgagccgt tcgatcaatt cctgaacttg cgactcgctg aagaagatcg tcggtacgac
       61 tacaaaagtc gacgtatttt ccgggatgcc ggtcaaagtc tcgattcttg gcagcagacg
      121 aggagggaag aaatgcgtca gatcccaatt gacgactgtg accgcaaggt cgctcgccgg
      181 aatcaaggcg agcaggccgg ctgcaacgag aacgagccaa cttgcaccgg ccagagacat
      241 cgcagttaac aggaacccga gcagcaagat agtcagaagt gcaataaccc cgagataaga
      301 ggcggttgcg tggcgcacca gaaaacgccg cagcttttcc ctggttcgtg gacgataacg
      361 aaagattgct tcgagctggg gtagtccttc gtcaatcagg tagtagccga cgtgcttttt
      421 agcttgtgca tcgttctcct ttgcctcggt tgcgagtttg acggtggcct gggcaatctt
      481 cagttcactt gtgcgagtcc gtttgctgat tcgttcgatg acgtgccggt agcgatcgcg
      541 cgaagcaaac tccatccgca aatacgcgcc ggccggatcc ttaccgagca acggttcgat
      601 tagactgact ttttcaaaga agtcgttcca gtcgagcgtc gagagcagac gcatgctggt
      661 gatgatgttg ccgaccgtta cctgcgcggc ggcctggcgc tggtgttcgg tgtgaatgat
      721 ctgttcgacg ctggcgcctt ggcgtcctaa ctgtttctcg atccaatcca tcacgggcat
      781 cacggacgga tgctgctcgc gtaaacgttg gatgagttgg accagaaacg tctgtgggag
      841 ttcttctcgt ttgccgagcc gttcggtgac ggtggtgttg atggccgtgg gttgcagcga
      901 agcgagttcg agcaacttgt cggcgagcag atcggcttct tcgcgttcag cgcgcgcacg
      961 agcgatgttg acggcgaggc gccgcagatt ctcgaccagc gccagacgaa gggtaatcgc
     1021 gacagcccag agttcaccga tcgacagcgg cgacaccgtt tgataagcag caatgaagcg
     1081 gcgcagggag ttggtgtcga gtcgtgagtc ggtgtgagtg attagctcca gggcgactgc
     1141 gtagattcgc ggatagccgg ccagttcacc gtcggtgagt ttgggcagtt cgtgatagta
     1201 gctctttggc agatcgtgac ggatctcgcg aagttgctct tcgacgatgt gatagttgtc
     1261 gaccagccac tcggcggctg gggagattgc gcggccctcg cggagcgctt cgaccaaagc
     1321 tttgtaggcg gattcgagta tgcggccgtt ttcggcgagg cgcggtaaaa gaatggcgcg
     1381 gcctttgcgc gtgacggttt tgtgttcggc ggccagcgct tgagcgtact gttcgagccg
     1441 ttcgacgctg tagaactcgg cgtggaatgg cttctcatca gtgtcgcggg ccggcttccg
     1501 aaacaacgag gcaaacgatt cgaagaactc gctcatgaac gtcacgacca agtaacaagt
     1561 tacaggtttt aggggatttt cagaatatca tgaagtgcta gttgtccgta gtcagttgtc
     1621 cgtggtgggt tgtccgttgt cagtgataag acgaggaact agaaggtctg gacaactgat
     1681 ccactgacca ctgaccacgg acaactgacc actgactgcg ttcaaaaggt agtatactct
     1741 cgcgccatgc gcgtgttggg aatcgatcct ggcagtcaaa cgatgggatg gggcgtgttg
     1801 gaaggtgacg ggcgtcgcta ctcgctcgtt gcgtatggtt ccgttcgtgc gcctgcgtct
     1861 ttgaagtttt ccgcgcggct gttgcggatg tgcaacgggc tcgaagaagt gattgccgag
     1921 caccggccgg atgcgtgtgc gcttgaagat gcttttctgg cgacgaacgt caaggtcagc
     1981 atgaagcttg ggcaagtacg gggcgtggtg ttgttggttg ctgaacgtgc tgcactggaa
     2041 attcacgagt attcgccgcg gttggtgaag cagtcggtcg tcggatacgg caacgcggaa
     2101 aagcatcagg tgcaggagat ggtgcgattg ctgctgtcgt tgaaaagtgt gccgtcgcct
     2161 catgacgctg cagacgcgtt ggcagttgcg atctgtcatt ttcatcacgc cccgtttaag
     2221 caaaggttgg ccgtattggt ttaagtggct ttgcccgaaa agtattcagc gaggcacttt
     2281 tcgagcaaag ccgatttaag tcttattaac ctcactcaaa ggagcacaga aatgaatcct
     2341 cacaaactca cgatccggct cttactctct ctagtcgtcc tttatttatg cgcgccggcc
     2401 gctgccgctc agccgcgtaa gccgccggca ggtggacgtc tcgccgttgt ggttgatgag
     2461 cggcttgcgg cgttgcgcgg cactccgcag ttgtgtggtc gcctcgtgcg ccgtctcagt
     2521 cgcgggcgaa tgattgcaat ccgtggttcc aaaacgacag ccgagggcgt agtcttcttc
     2581 ttagtaaacg taaccagacg cactcggggt tggattcagc gtgaagcagt tgttgcggtt
     2641 ggtcgtgccg gcgaagatcg ccgcttgctg gaactgataa acgcatcgta cggtttcgac
     2701 cgaacggcgc gtgcacggat cttcttggat tactttccgc gttcgccgtt gcgtccgcgc
     2761 gtgttgttgt tactggggaa tgctgccgag gaacaggccg cggaactttc acgtgaggcc
     2821 gcgcggcgtc tgaacgcaac aatgggttcg gcgcccgagt ttagttactt cctgaactac
     2881 agcgggctgg accgttacaa ccgccagcgt gttggctttg tttttgaccg tgcgacgaga
     2941 cgttttcact atgacggcgc cgcctggcgc gaactggtcc gtcgctaccc aaactcagcc
     3001 gaagccgccg aagctcgcca acatttggac gagttggcgg caatttcgca gtaaaattca
     3061 cgagcacggc tgttaataaa ccctttacgc ccccgtctat caagtgtagg acgaaggaga
     3121 acaccccgtg caggctgctc ttaaaccttt gcgactcgtg aaaaatgaac ctgcgggaga
     3181 gctggaaacg ctcttccaga cgcatcatgg ccgcgttttt cggaccgccc aacgcatcac
     3241 cggcagcgcc gcggatgccg aggacgtatt gcaaaacgtc tttctgcggc tggtaaaagg
     3301 gcaggaaacc tacgatctgt cgcgcaaccc ggaagcctat ctttcccgcg ccgctatcaa
     3361 tgcttcactc gatctgttgc ggagtcgcag cagagccagg tcagtcggcc tcgatgatgc
     3421 agacgctgat acgcttgcgg gcggcgcccg cagccccgag tcgctgcatg ccgaccgaga
     3481 actgcaatcc ctcgtgcgcc aggctgtttc ccggctcgga aagaccgcgg gcgaaatgtt
     3541 tgtcctccgt tattacgaag gtttaggtaa caaagagatt gccgaactgc tggaaacctc
     3601 gccgttcgtc gtgggcgtcg ttctgcatcg cgctcgcaca aaactgagaa aagagattgg
     3661 gcactatctg gagaaacacc atgaaaagga atagtgaaga gatcctcgac aaagttactg
     3721 ctgagattcg ccacgagaac gtcgatccgg cagtggtttc cgcggcggca gaccgtgttt
     3781 gggcgcgtgt ctctgtcgca gcgggcgaga ctgaagttca aatgccggcc gtcgatcgga
     3841 ttgaaggctg tgccgatttt cagtcgctga ttccggctta cctggcagga aagctttcgg
     3901 aagcgcggtc gatgttgctg gtggaccaca cgcacgaatg catcccttgc cgtagggcga
     3961 tgaaggaagc gcgttcgcgt aaaacggttg tcgccaaagc gccggctaag ccgagccgct
     4021 actcgcttca gccggtggtc atgcgctggg gcattgccgc tgcactcgtg atcggtttcg
     4081 gcctgctggc cgtgcctttc gtgcagcgct actggccgtt tggtgagttt gaagcaacgg
     4141 tccaggcggc tgaaggtcag gtctatcaag ttgccgacac gcgtaccgca gccgtgagca
     4201 ctggtgcgaa gttgcaaaaa ggcgaaaagg tccgcaccgc gaaagatgcg cacgccgttg
     4261 ttcgcctcag tgacggctcg ttgatcgaaa tgaaagagcg ttccgaactc tatctgacaa
     4321 agaccggaca aggaacgacg atccatctcg atcgcggcag catcgttgtc gaagccgcga
     4381 aacagaacga cggaaagttt tttgtcgaga gtggtgattc gctcgtgtca gtgacgggaa
     4441 ctgtgttctc ggtcaacaac tgcacgaaag gatcgcgggt ttcggtaatc gaaggtgaag
     4501 taaacctgaa ccacgcaggc agtgatcgcg tgttgcgtcc cggcgaacag gctacaacca
     4561 gtccgtcaat tgcgacaatt ccgattaagg atgaagttgc gtggagcaag aacgcgagtc
     4621 gttatgcgtc gttgctgtcg ggcctggcga cactcaagaa cgagctgcaa acagtctcgc
     4681 agccgggcgt tcgcaattcg acgcacctgc tcgacttgat gcctgagaac acggtggtct
     4741 acgccgcact gccgaatttg accaacacca tcacggaatc gcaccgcatc attcaggagc
     4801 ggatgagcca gaatgcggcg ttgcgcgcgt ggtgggaaaa agagcagtcg ggcagagcgc
     4861 aaaacatgga ccaggtggtt cagacgattc gcgagtttgg ttcgt
//
LOCUS       DASTEY010000627         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1596400, whole genome shotgun sequence.
ACCESSION   DASTEY010000627 DASTEY010000000
VERSION     DASTEY010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596400"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /locus_tag="VFO72_10940"
     CDS             <1..2533
                     /locus_tag="VFO72_10940"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET9479853.1"
                     /translation="ILNNQPFQFSPAKFIRPLGWMVSAVVGLIYGFKLKEQWRQFALY
                     LHQPPTSTYDPIFGKPLGFYLFSLPFYDLLSSWLFGVTVIILFAALAYSLLGLPQTVL
                     KPSVRWSSGAAFRGVCCALALVLLVLSWRTYLARFPYLWQDHPIFAGVTYTEAHYTLP
                     ALLVVAVALIIGAIISLVNAFTVRRFSLLMIALALPLAVYIVGVVLVPAYIQGFIVKP
                     NELDRETPYIAHNLEWTRRGFGLDRIEFRDFEADPSIAGLDLANNRATLDNIRLWDWR
                     ALQDTLRQIQAIRTYYDFADVDVDRYLVAGQARQMMIAPREINDAKLPASSRNWINER
                     LIYTHGYGVTMNSANGFTPEGMPQFVLSNMPVESTAPEIKITRPEIYYGELTDRYVYV
                     NTKQNEFDYPQGDTNTYTTYQGNAGIRIGNRLRRMLLAWGLGDLSKLPFSNDISSESR
                     VLINRNIRNIVDGVAPFLIYDKDPYTIVDNEGRLFWIIDAFTESAYFPYSSHHGVEGD
                     SINYIRNSVKVVIDAYHGTTNFYIFDKQDPLIAAYQRVFPALFRDANEMPADLRAHIR
                     YPETLLRAQGEVYNLYHTQNPSIFFQREDVWNVAQRLTLDKDGKKQTTPIDPYFVLMQ
                     LPGEPQTNEFVLILPFTPVNRNNLIGWIAGRSDGENYGKLLVYNFPKSRLIDGPLQIE
                     ARIDQNSQLSSQFTLWNQQGSRIIRGHLLVIPIGRSVLFVEPIYLQAEHSPMPELRTV
                     VLATHEKLAHGATFNEALTNLAGETAREVQPGQTGAPSPPSGETGQPAPTETPSQSLQ
                     QLVNRAIQEFDDYQRLTAEGKHGEAGQKLEQHRRTLEEIRRLTRP"
     gene            complement(2542..3240)
                     /locus_tag="VFO72_10945"
     CDS             complement(2542..3240)
                     /locus_tag="VFO72_10945"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET9479854.1"
                     /translation="MALSTSDAKVAAERIAEADALYDGREDLNKARVAVAALRQAWTA
                     DNGNYEAAWKLARAAFYVGEHTDNDSERDDMFREGIEAGKAAIAIQPDKPDGHFWLGA
                     NYGGSAAHSTLANLSSFQDIKSEMEAVLKIDESYQGYSAYLGLGRLYLQAPRVLGGDS
                     AKAIEYLEKGVKLNPNNTMMRLHLAEAYEANNRTGEARKQIDAIMAATPDPKYTSEHN
                     DAMAKAQKLLEKIS"
     gene            3500..4162
                     /locus_tag="VFO72_10950"
     CDS             3500..4162
                     /locus_tag="VFO72_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9479855.1"
                     /translation="MAGAAFYDLEGTLVSTNLVHTLSFYAGRQQGLMASLKKSAATYL
                     SLPLFAVTDQYSRKVFNDLYFRRYKGQSEDRLRYFAQELFEDVIKPAVFPGTYELIEK
                     SRKLGLRQVVITGALDLSVKPLMEHLGITEYVTNRLEFVNGFATGRLLPPVMAAATKA
                     SWIRTYTEREGISLSDSYAYTDSLSDLPMLSIVGHPAVINPDMRLRQTALHHDWPILN
                     LK"
BASE COUNT          888 a         1211 c         1114 g          965 t
ORIGIN      
        1 cattctcaac aatcagccgt ttcaattctc gccggcaaaa ttcatccggc cactgggttg
       61 gatggtttcc gcggtcgttg gactaattta tgggtttaag ttgaaggaac agtggcggca
      121 gttcgcgctc tatctgcacc agccgccaac ctcgacgtac gatccgatct tcggtaagcc
      181 gctcggtttc tatctattct cgttgccgtt ttatgacctg cttagttcgt ggttgtttgg
      241 cgtcactgtc atcattcttt ttgcggcgct cgcttattca cttctggggc tcccgcagac
      301 tgtgctcaag ccgagcgttc gctggtcatc gggcgcagct tttcgtggcg tctgctgtgc
      361 gctcgccctg gtcttactcg tgttgagctg gcgcacctat ctcgcgcgct tcccgtatct
      421 ctggcaggac catccgatct tcgcgggcgt cacttacacc gaagctcatt acacgcttcc
      481 ggcgctgttg gtggttgcag tcgctctgat catcggcgca atcatctcgc tcgtgaatgc
      541 ttttaccgtt agacgtttca gtctgctgat gattgcattg gcgctgccgc tggccgttta
      601 catcgtgggc gtggtgctgg tgccggctta catacagggc tttatcgtca aaccgaacga
      661 gctcgaccgc gaaacgcctt acattgcgca caacctcgaa tggacgcgcc gcggctttgg
      721 cctcgatcgg atcgagtttc gcgacttcga agcggacccg tctatcgccg gcctcgatct
      781 cgccaataat cgcgctaccc tcgataacat tcgcctgtgg gattggcgcg cgctccagga
      841 caccttgcga cagattcagg ccattcgcac ctattacgac ttcgcagacg tcgacgtgga
      901 tcgctacctc gttgcgggcc aagcgcgcca gatgatgatc gcgccgcgcg agatcaacga
      961 cgcgaaactt cccgcatcat cgcgcaactg gatcaacgaa cgattgatct acacgcacgg
     1021 ctacggcgtg acgatgaata gcgccaatgg cttcacgccc gaaggaatgc cgcagtttgt
     1081 gttgtcgaat atgccggtcg agtcgacggc gccggagatc aaaatcaccc ggcccgagat
     1141 ctactacggc gagttgacgg accgttacgt ctacgtcaac acgaagcaga acgaattcga
     1201 ttatccgcaa ggcgacacga acacgtacac gacgtaccag ggtaatgccg gaatcagaat
     1261 cggcaaccgc ctgcgacgaa tgttgctggc gtggggtctt ggtgatcttt cgaagctgcc
     1321 gttttcgaat gacatatcca gtgaaagtcg cgtcctgatc aatcgcaaca ttcgcaacat
     1381 cgtcgatggt gtggcgccgt ttctaatcta cgacaaggat ccttacacca tcgtcgacaa
     1441 tgaaggccgg ctcttctgga tcattgatgc gtttaccgag tcggcttatt tcccatactc
     1501 gagccatcat ggcgttgagg gcgactccat aaattacatt cgcaacagtg tgaaagttgt
     1561 gattgatgct taccacggca caacaaactt ttatatcttc gacaaacagg atccgctgat
     1621 agctgcgtac cagcgcgtct tccctgctct atttcgcgat gccaatgaga tgccggcgga
     1681 cttgcgagcg catattcgtt accccgaaac actgttgcgg gcgcagggcg aagtctacaa
     1741 tctctatcac acgcagaatc cgagcatctt ctttcagcgc gaagacgtct ggaacgtcgc
     1801 gcaacggctc acactcgaca aagacgggaa aaagcagact actccgatcg acccctattt
     1861 cgtgttgatg cagttgccgg gtgagccgca gacaaacgag tttgtgctga tcctgccgtt
     1921 cactccggtc aatcgcaaca acctcatagg ttggatcgcc ggtcgcagtg acggagaaaa
     1981 ctacggcaag ttgctggtct acaactttcc gaagtcgcgc ttgatcgatg gtccacttca
     2041 gatcgaggca cgcatcgacc agaactcgca gctttcgtca cagttcacgt tgtggaacca
     2101 gcagggatcg cgaattatac gcgggcatct gctggtaata ccgattgggc gttcggtgtt
     2161 gtttgtggag ccgatttatc tccaggcaga acacagtccg atgccggagt tgaggactgt
     2221 ggttttggca acgcacgaaa agctcgcgca cggagcaacg ttcaacgaag cactgactaa
     2281 tctcgctggt gagacggcgc gggaagtgca gccgggccaa acaggtgcgc cgtcaccgcc
     2341 ttccggcgaa acaggccaac ccgcgcccac cgagacgcct tcgcagagtt tgcagcagtt
     2401 ggttaacaga gcaattcagg agtttgacga ttaccagcgc ctgactgcgg aaggcaaaca
     2461 cggtgaagcg ggccaaaaac tcgaacaaca ccgtcgcacc ctcgaagaga tccgccgcct
     2521 cacgcggcca taaccactga tttaactgat tttctccaac agtttctgcg ctttcgccat
     2581 ggcgtcgttg tgctcggacg tgtatttcgg atccggcgtc gctgccatga tggcgtcgat
     2641 ctgtttcctg gcttcgcctg tgcggttgtt tgcttcgtag gcttcggcga ggtgaagtcg
     2701 catcatcgtg ttgtttggat ttaacttcac tcccttttcc aaatactcaa tcgcctttgc
     2761 ggagtcgccg ccgaggacgc gcggcgcctg aagatataag cggcccaacc ctagataagc
     2821 actgtaaccc tgataactct cgtcgatctt cagcaccgcc tccatctcac tttttatgtc
     2881 ctgaaaactt gaaaggttcg cgagcgtgct gtgcgctgca ctgccgccat aattcgctcc
     2941 caaccaaaaa tgaccgtccg gtttgtcggg ctgaatcgca atagccgcct tgccggcctc
     3001 gatgccttcg cgaaacatat cgtctcgttc ggagtcgtta tccgtgtgct cgcccacgta
     3061 aaaagccgct cgagccagct tccacgctgc ctcgtagttt ccgttgtcag ccgtccacgc
     3121 ctgccgcagg gcggcaaccg ccacacgcgc tttattcaga tcctcccgcc catcgtagag
     3181 cgcgtcagct tcggcaatac gctcggcagc aaccttagca tcgctcgtac taagcgccat
     3241 cgggtccgcc ggcggcgact ggctgcagcc caaggtcaac aagaaccacg aaaaagcagt
     3301 aaaaacgatc atcacagatc gcactttttc gtggcttttt ccaggatttc ccattaatcg
     3361 acctccgatt gtctagtatc gccagacagt ttgtaatact tttgcgtaca cgctaacgaa
     3421 acgacgggga atacggtata gtggcttgcc gttcggcgcc acgcacgtct aaacacgccg
     3481 ggtctgcttg gaggagagct tggctggggc agcattctac gatcttgaag gcactttggt
     3541 gagcaccaat ctggtgcata cgctctcgtt ttacgccggc cgccagcagg gactgatggc
     3601 cagtttgaag aagagcgcgg ccacttacct gagcctgccc cttttcgcag tgacggacca
     3661 gtatagccga aaagttttta acgacctcta cttccggcgg tacaagggcc agtcggaaga
     3721 ccgtctgcgc tacttcgcgc aggagctgtt cgaggacgtg attaaacccg cggtttttcc
     3781 cggcacctac gagttgatcg aaaaatcgag aaagttggga ctgcggcagg tagtaataac
     3841 cggtgcgctc gacctttcag tgaagccgct gatggagcac ttagggatca ccgagtacgt
     3901 gacgaaccgg ctcgagtttg taaacggctt cgccaccggt cgcttgctgc cgccggtgat
     3961 ggccgcggcg acgaaagcgt cgtggatcag gacctacacc gaacgcgaag ggatcagctt
     4021 gagcgactcc tacgcctaca ccgacagctt gagcgatcta ccgatgttgt cgattgtggg
     4081 tcacccggcg gtaattaacc cggacatgcg cctgcgacaa accgcgctac atcacgactg
     4141 gccgatactg aacctgaaat gattgccgat tgccgatt
//
LOCUS       DASTEY010000628         3734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_689763, whole genome shotgun sequence.
ACCESSION   DASTEY010000628 DASTEY010000000
VERSION     DASTEY010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689763"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /gene="lepB"
                     /locus_tag="VFO72_10955"
     CDS             complement(<1..467)
                     /gene="lepB"
                     /locus_tag="VFO72_10955"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9479856.1"
                     /translation="MASNRTTDKITPGATPTKTEEKPFSFSRLFNRNGHEKNTPPPRV
                     QGPPKTVWREYFESAVVTVVMALFGMTFIVQAVKVPTGSMQNTITIGDHLLVNKFIFA
                     PGESLPFLPQREIRRGDIIVFKYPGNKYEPDRLERADNIPILTNYVKRVIGLPG"
     gene            complement(529..1305)
                     /gene="rnc"
                     /locus_tag="VFO72_10960"
     CDS             complement(529..1305)
                     /gene="rnc"
                     /locus_tag="VFO72_10960"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET9479857.1"
                     /translation="MKNPETIPDLELLETVLAYNFNDRTLLERAITHRSWAHEKVAPG
                     DERQARKLHNESLEFLGDSVLGLVVAHYLCNSYPAGTEGELSRMKHRLVSAPTLAEAS
                     QGLKLGDFLRFGRGEEKSGGRQKNALLADVFEAITGAIFLDGGLEAATQFVCRALRAE
                     LETADPLSAAKADYKTMLQEKLQAERRPAPRYSVIETLGPPHQRVFHVEVAWDSGSIR
                     GSGHSIKAAEAAAARAALEAMTAELGADVVEAARESTDAV"
     gene            complement(1398..2570)
                     /locus_tag="VFO72_10965"
     CDS             complement(1398..2570)
                     /locus_tag="VFO72_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479858.1"
                     /translation="MMKLVMIGATVALLAVPVAATNLITDNSVQDQCKQEEKDAWYAE
                     FLKIRTTDQPKAFELAKKYLACPATGEVTEPQQKIIDYLKKWSTAYEEGMRKVKLPQL
                     LYNEKKYPEAYALGREILAAEPENLKVLVDLGANGYVVAPLKNPQLITESLDYARRAL
                     QLIESGKTVDDWTPLASKEAALAYLNFSIGTLTLEKDPANALKNLIKAAQFETPLKKS
                     PFTYALIAAAYETGPYAKMSEAYKMTYGGKDETPESKLALANINQIVERMIDGYARAV
                     ALAGGDQQFATQKPVWLESLTTWYKYLHNGSDAGMNEMIASVVAKPLPPEPTPLTSLP
                     PSTPATTPIGNTGAAGATGNGASSTTTGTATKTGTPATGTPSKPKPGTPDRPRKRQ"
     gene            complement(2879..>3734)
                     /locus_tag="VFO72_10970"
     CDS             complement(2879..>3734)
                     /locus_tag="VFO72_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9479859.1"
                     /translation="FEFPGKLPELTPGFSERRMFSHADGFQVTPLQLGTMVSAMANGG
                     RLLVPQIPRSTKDQSKMSPKVRRVLDISNEVWQRMVPGMVGAVNYGSGRKAYDPTQTV
                     AGKTGTCIGDNGWVGLFASYAPLANPRLAVVVIAEGTDARRHFPAAVAGEIYRQLNHR
                     FGTAINLQVASTLDDEEKEVADAEAAAENTEAGEGEESTAVATPATGSTQDANVKTAP
                     SVPMPSTTTAKPGTAEPKNTVKRVLMPLNNKTSDAQKNGSQSDSKTPTAPANTTAPGD
                     QRPRRIQP"
BASE COUNT          753 a         1048 c         1038 g          895 t
ORIGIN      
        1 cccggcaaac cgatcactcg tttcacgtag ttcgtaagaa tcggtatgtt gtccgcgcgc
       61 tcaaggcgat cgggttcata cttattaccg ggatacttga agacgatgat gtcgccacgc
      121 ctgatctctc gctgcggcag aaacggcagc gactcgcccg gcgcaaagat aaacttgttg
      181 accagcagat gatcgccaat cgtgatcgtg ttctgcatcg atcccgtcgg caccttcacc
      241 gcctgcacga tgaaggtcat accaaacaac gccatcacga cagtgaccac agccgactcg
      301 aagtattcgc gccagacagt cttcggcggt ccctgcactc gtggcggcgg agtgttcttt
      361 tcgtgaccgt tccgattgaa tagacgcgag aaagaaaaag gcttctcctc ggttttcgtc
      421 ggagtggcgc cgggcgttat cttgtccgtg gttcgattac tggccattga aaaaacatcc
      481 tagatacgtt gctggcgatg gtcaagctgc gaccgcagac atctgatttt agactgcatc
      541 agtgctctcg cgcgctgcct cgaccacgtc tgcgccgagt tctgcggtca tcgcctccaa
      601 cgccgctctg gcggccgccg cttccgcggc tttgatcgaa tggcccgacc cgcgaatgct
      661 accgctgtcc cacgcgacct cgacgtggaa aacgcgttga tgtggcgggc ctaacgtttc
      721 gatcaccgaa taacgcggcg ccggccgccg ctcagcctga agcttctcct gcaacatcgt
      781 tttgtagtcc gcttttgcgg cgctcaacgg atcggcggtt tcgagctctg ctcggagtgc
      841 gcggcaaacg aattgagtcg cagcttcgag cccgccatca aggaagatcg ctccggtaat
      901 cgcttcgaac acatccgcca gtaacgcatt cttctgccgc ccgccactct tttcttcacc
      961 gcgtccgaaa cgcaggaaat caccgagctt taacccctgc gatgcttcgg cgagcgtcgg
     1021 tgcactcacc aaccggtgct tcatgcggga cagttcgcct tcggtgccgg cgggataaga
     1081 gttgcaaaga tagtgtgcga cgacgagacc gagcactgaa tctccgagga actcgagcga
     1141 ctcgttgtga agcttgcgcg cttgccgttc gtcacccgga gcaacttttt catgcgccca
     1201 cgaacgatga gtgatcgcgc gttcgagcaa agtacgatcg ttgaagttgt aggcgagaac
     1261 cgtttcgagg agctcgaggt caggaatcgt ttcgggattc ttcaaagcgc agcggattat
     1321 aacaaacgcc ggaagcttgt cgaaaaacgc gacgtgcttc cggcgttgga aaacggtcgg
     1381 tggaaaatta acttccgtta ctggcgcttg cgtggtcgat cgggagttcc gggcttcggc
     1441 ttactcggtg taccagtcgc aggcgtgcct gtcttggttg cggttcccgt cgttgtgctt
     1501 gaggcgccat tgccagttgc ccctgctgca ccagtgttac cgatcggagt agtagccggt
     1561 gttgagggtg gcagcgatgt cagcggagtc ggctccggtg gcaacggctt ggcgacgacg
     1621 ctggcgatca tctcattcat tccggcgtca gaaccgttat ggagatactt gtaccaggtt
     1681 gtcaggctct cgagccaaac tggcttttgc gttgcaaact gctgatcgcc accggcaagt
     1741 gcaacagcgc gcgcatagcc gtcgatcatc cgttccacga tctggttgat gttggccagg
     1801 gccagcttgc tttccggagt ctcatccttg ccgccgtagg tcatcttgta cgcttcagac
     1861 atcttggcat aagggccggt ttcgtaagca gcggcgatta atgcgtaagt gaacggcgac
     1921 ttcttcaacg gcgtctcaaa ctgcgctgct ttgatcaggt tcttcagcgc gtttgcagga
     1981 tccttctcga gcgtcagcgt gccgatgctg aaattcaagt aagcgagcgc ggcctctttg
     2041 cttgcgagcg gcgtccaatc atcgacagtc ttgccgctct cgatcagttg cagtgctcgg
     2101 cgggcataat ccaacgactc agtgatcaac tggggattct tcaacggcgc aacaacgtaa
     2161 ccgttagcgc cgaggtcgac aaggactttc aggttctccg gctctgccgc gagaatctca
     2221 cgcccgagtg cgtaagcttc agggtatttc ttttcgttat aaagaagctg gggcagtttg
     2281 actttcctca tgccctcttc gtaagcggtg gaccacttct taaggtagtc gataatcttc
     2341 tgctgcggtt cggtgacttc accggtcgct ggacaagcga gatatttctt tgccagttcg
     2401 aaagctttcg gctggtcggt ggtgcgaatt ttgagaaact cggcgtacca cgcgtccttc
     2461 tcctcttgct tacattgatc ctgaacgctg ttatccgtga tcagattggt cgcagcaacg
     2521 ggcacagcga gcaacgcgac tgtcgcaccg atcattacca gtttcatcat ctttgtcatt
     2581 agtggtcgga acctccaaaa atcttctttc agcaatcgcg ctataggctg agccctttca
     2641 agcttcgttt acaggaacaa taggatgcaa gtgatacaga gaccggttgc cgcgtctgcg
     2701 catcgaggga atgggctctg gtctagactt agtaaaaaaa gaacgcccgc aatattcctg
     2761 caactctgtt aaaagtgtca aggcgcgctg agcgcttgtc gcagaaaacg tcgcaaaaaa
     2821 atttgcgccg gatatgaagc ttccggcgca aaacaatcaa aactgtgctt ttagtctgtt
     2881 atggctgaat acgtcgtgga cgctgatcgc ccggcgcagt ggtgtttgcc ggagcggttg
     2941 gcgtcttgga gtccgattgt gaaccgttct tctgtgcgtc agacgttttg ttattgagag
     3001 gcatcagcac gcgtttcacc gtgttcttcg gctcggccgt cccaggtttt gctgtggttg
     3061 tagacggcat cgggacggac ggcgctgtct ttacgttcgc atcttgagtt gagccggtag
     3121 ctggcgtagc aacagcagtt gattcttcac cttcgcccgc ttcggtgttt tcggcggcgg
     3181 cttcagcatc cgcaacttcc ttctcttcgt cgtcgagtgt ggaagcgact tgcagattga
     3241 tcgcagtgcc aaagcgatga tttaactggc gatagatctc gccggcaact gccgctggga
     3301 aatgacgacg cgcgtcagtg ccttctgcga ttacgacgac tgccagtcgt ggattagcaa
     3361 gcggcgcgta ggaagcaaac aatcctaccc aaccgttgtc gccgatgcag gtaccagtct
     3421 tgccggcaac tgtctgcgtt ggatcgtaag ctttgcggcc cgagccgtag ttcacggcgc
     3481 caaccattcc cggcaccatc cgctgccaaa cctcattaga gatgtccaac acgcggcgaa
     3541 ccttcgggct catcttgctc tgatctttcg tgctgcgcgg aatctgtggc accagtaaac
     3601 gaccaccatt ggccatcgct gaaaccatcg ttcccaactg caacggcgtc acttgaaaac
     3661 cgtccgcatg agagaacatt cggcgctcgc taaatccggg cgtcagttcc ggcagcttgc
     3721 cgggaaactc gaag
//
LOCUS       DASTEY010000629         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1184072, whole genome shotgun sequence.
ACCESSION   DASTEY010000629 DASTEY010000000
VERSION     DASTEY010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184072"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFO72_10975"
     CDS             complement(<1..718)
                     /locus_tag="VFO72_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9479860.1"
                     /translation="MSQNAFSKREERAEPSARPVELHSDLYREIFDHSKEAIAIIDRD
                     GFYLQQNGAHFTLLGYPDDDLKGKTLADELDAATFAQLQRQLVEADEFSGEIVCRSKN
                     GEELNIDLAVFTMRSGLGEPLCYVCIKRDITKRKQDELALRRSQSELTDFFENASTGL
                     HWVSADGTILRANQAELDLLGYTREEYVGRNIVDVHTDREVIEDILERLRAGEVLRDY
                     EARLRCKDGSIKIVRIDSSAY"
     gene            complement(844..1878)
                     /locus_tag="VFO72_10980"
     CDS             complement(844..1878)
                     /locus_tag="VFO72_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9479861.1"
                     /translation="MTNIRVGVIGAGYIGGVHAGILARDERVQLAAVYDIDRERAERL
                     ARAHDASVAAGPGELIAQCDAVYITTPNTQHVQLALEAIDAGKHVFCEKPLATNIADA
                     GTVFAKAKSSRGVFQVGHNRRFAPVYSTLKQLLSEKHVPHSAHVKMNRGELLNPVWTG
                     DPSVTGGFLYETPIHMFDLMRFLFGEIDSLHAIGSKHEYRETDDFSVLLKFTSGFHAT
                     LATAADASWLFPFERVEVFCHHATFVTREMETLVCSTNLEGHYAEQSMHQLPREEKWG
                     YAQEDRAFVDAIVSGTPPPVTVLDGLMSVELVNAVYESVKTDATIAVRSLERPVPGTA
                     GVSPAPEGGT"
     gene            complement(1871..2701)
                     /locus_tag="VFO72_10985"
     CDS             complement(1871..2701)
                     /locus_tag="VFO72_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET9479862.1"
                     /translation="MQIALNGATTMQADLAADFQAAKAAGFDYVEIWAEKLVTFLHHR
                     TSLELKDLIAKTVPPLSINSIEHITFSTPFTWDAIKKQCAELSDIAARLDCPYLVVVP
                     SPLPNSGVTRSEVIAESVRVLRELCDIAARSNTSLAFEFIGQSDCSVPTLDLAHEIVI
                     TANRPNLGLVIDSFHFYAGGSTIEMVEALDPELIYIFHINDAENLPREQLQDSHRLLP
                     GLGILPLRELLAAFRRIGYDKVASVEIFRPEYWERDPFELAKEARIATERVLQAKPND
                     "
BASE COUNT          618 a          827 c          872 g          688 t
ORIGIN      
        1 agtaagcact cgagtcgatg cgcacaattt ttatcgaacc atctttacaa cgcagccgag
       61 cttcgtaatc gcgcagcacc tcgccggcgc gcaaccgttc gagaatgtct tcgataactt
      121 cgcggtcggt gtggacgtcg acgatgttgc ggccgacgta ctcttcgcgc gtgtatccga
      181 gcagatcgag ttcagcctgg ttcgcgcgaa ggatcgttcc gtcagcgctg acccaatgca
      241 aaccggtcga ggcgttttca aaaaagtcgg ttaactctga ctggctacgg cgtaaagcaa
      301 gttcgtcttg tttgcgtttg gtgatgtcgc gtttgatgca gacgtaacag agcggttcac
      361 cgaggccgct gcgcatggtg aacacagcca gatcgatgtt tagttcttca ccgttcttcg
      421 agcggcaaac aatctcgccg gaaaactcgt ccgcctcgac gagttggcgc tggagttgtg
      481 caaacgttgc tgcgtcgagt tcgtcggcaa gagtcttgcc cttcagatcg tcatcgggat
      541 aaccgagcag cgtgaaatgg gcgccgttct gttgcaggta gaacccgtca cggtcgatga
      601 tcgcgatggc ttcttttgaa tgatcgaaga tttcgcgata gaggtcggaa tgcagttcaa
      661 cgggccgagc tgatggctcg gcacgctctt ctcgttttga gaaggcattc tgcgacatga
      721 aactccccta acgaagcgga tccaggtcaa gagggggtct cggtcgggtt ggagggatat
      781 tagcattaac ctaaggggct cttaaacctg ggaccgcagg cgtctcgccg gcacgccggc
      841 gaatcaggtg ccgccctcag gtgcaggcga gacgcctgcg gtcccaggaa ccggtctctc
      901 aagcgatcgg accgcaatgg ttgcatcagt cttcactgac tcgtaaacag cgtttaccag
      961 ctcaaccgac atgaggccgt ccagaacggt caccggcggt ggcgtgccac tgacaatcgc
     1021 atcgacgaag gcgcgatcct cttgcgcgta gccccatttc tcttcgcgcg gcaactggtg
     1081 catcgattgt tccgcgtagt gtccttcgag gttggtgcta cagaccagag tttccatttc
     1141 gcgcgtcacg aatgtcgcgt gatggcagaa cacttccacg cgttcgaacg gaaacagcca
     1201 gctcgcgtcg gcggcggttg ccagcgtcgc atggaatcca ctggtgaact tcaagagcac
     1261 tgagaagtcg tcagtctccc ggtactcatg ctttgagccg atcgcgtgca agctatcaat
     1321 ctcaccaaac agaaatcgca tcaggtcgaa catgtgaatg ggcgtttcat aaaggaagcc
     1381 gccggtgacg gatggatcgc cggtccagac cgggttcagc agttcaccgc ggttcatctt
     1441 cacgtgcgcc gaatgcggca catgcttttc gctcaggagt tgtttcagag ttgaatagac
     1501 cggagcgaag cggcggttgt gtccgacttg aaacacgccg cgactactct ttgctttcgc
     1561 gaagacggtt cccgcgtccg cgatgttcgt ggctaacggt ttctcgcaga agacgtgctt
     1621 gccggcgtcg atggcttcga gcgcgagttg gacgtgttgc gtgtttggcg tggtgatata
     1681 aaccgcgtcg cactgtgcga taagttcgcc tggaccagcc gcaacgcttg cgtcgtgcgc
     1741 tcgcgcgagt cgttccgcac gctcgcggtc aatgtcgtaa accgccgcaa gctgcacccg
     1801 ttcgtcacgt gcgagtattc cagcgtgaac accacctatg taaccggcgc caataacacc
     1861 aactctgatg ttagtcattt ggtttggctt gtaagacacg ctcagtcgcg attctcgcct
     1921 ctttcgccaa ctcgaacgga tccctctccc agtactccgg ccggaagatt tcaacgctgg
     1981 caactttgtc gtacccgatc ctccgaaacg cagccagcag ttcccgcaac ggcagaatgc
     2041 caaggccggg cagcaggcga tgactgtctt gcaactgttc gcgcggcagg ttttccgcgt
     2101 cgttgatgtg aaagatgtag atcagttctg gatcgagcgc ttcaaccatt tcgatcgtcg
     2161 agccacccgc gtaaaagtgg aaactgtcga tcaccagtcc gaggttcggg cgattcgccg
     2221 tgatcacgat ttcgtgggcc aggtcgagtg tgggcacgct acagtccgat tggcctataa
     2281 attcgaacgc cagcgacgtg ttgctgcgcg cggcgatgtc gcacagttcg cgcaacacgc
     2341 gaactgattc tgcaatcact tccgagcgtg tgacgccgga gtttggcagc gggctcggga
     2401 ctacgacgag gtaaggacag tcgagccgcg cggcgatgtc gctcagctcc gcgcattgtt
     2461 ttttgattgc atcccaggtg aacggcgtgg aaaacgtgat gtgctcgatc gagttgatac
     2521 tgaggggcgg taccgttttt gcgatcaggt ctttgagttc caggctggtg cgatggtgca
     2581 ggaacgttac gagtttttcg gcccagattt cgacgtaatc gaagccggcc gcctttgctg
     2641 cctgaaaatc cgcggccaga tcggcttgca tcgtggtggc gccgttcagt gcaatttgca
     2701 tcgccgaaga atagcacacg gacaggtacg cggttcgttt gacttgccct ccgtatagtt
     2761 gtaaaagcgt ggtggcttcc gaacaactta atctgcgaac cttatcgtcc cggtctggtg
     2821 accgggagtt agtttttcga cagcaaaagg agacgaaaat ggctctacga cttggcgata
     2881 tagcacccga tttcacggca gatactacgg aaggtcccat ccgcttccat gaatggattg
     2941 gcgatagttg ggcgatcctg ttctcgcatc cgaaagactt caccccagtt tgcaccaccg
     3001 aactc
//
LOCUS       DASTEY010000630         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_888216, whole genome shotgun sequence.
ACCESSION   DASTEY010000630 DASTEY010000000
VERSION     DASTEY010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888216"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1340
                     /locus_tag="VFO72_10990"
     CDS             105..1340
                     /locus_tag="VFO72_10990"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET9479863.1"
                     /translation="MPVVSERWDVERIRADFPVLHQTINGKPLIYLDNSASSQVPQVV
                     IDRGSIYIEQEHSNIHRGVHYLSQKATTAYEGAREKVKRFINARDVRECIFVRGTTEG
                     INLVMHGYGRKFINAGDEIIISAMEHHSNIVPWQMLCEEKGARLRVIPMNDAGELLLD
                     EYDGLLNERTKMVAVMHVSNALGTINPIKEMIAQAHKYGVPVLIDGAQAAPHMPVDVQ
                     DLDCDFYVFSGHKMFAPTGSGVVYGKLEILETMNPFQGGGDMIKTVTFEKSIYADPPT
                     RFEAGTPAIASQIGLGAAIDYLNSIGRERAAAYEAELLRYATERVSAIEGVRIIGTAK
                     HKASVLSFVIDDIHPHDIGTILDQEGIAVRAGHHCAQPVMQRFNVPATARASFAFYNT
                     REEVDVLARTIERVIEIFS"
     gene            1346..1795
                     /locus_tag="VFO72_10995"
     CDS             1346..1795
                     /locus_tag="VFO72_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET9479864.1"
                     /translation="MSELSELYQQVILDHNKKPRNFRKLENANHTAEGYNPLCGDHLT
                     VYLALENDLVKEVGFEGSGCAISKAAASMMTQAVKGKSKQQAEDLFQEFHSMVTGELN
                     EEMEENSLGNLKIFAGVREFPVRVKCATLPWHTLHAALNKKDQVSTE"
     gene            complement(2058..>2289)
                     /locus_tag="VFO72_11000"
     CDS             complement(2058..>2289)
                     /locus_tag="VFO72_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479865.1"
                     /translation="ITDAVIRLLVYKISDNVVGDAVDVQSSGAASTGNLFRFENGEYV
                     FNLSTAGLTPGTYQLQVDMGDGVLRAINITLK"
BASE COUNT          559 a          644 c          632 g          454 t
ORIGIN      
        1 cgtggccgcc cgtcgtcgat gaaaacgggc ggccacggag tgccgcccct acaaaacgcc
       61 gggagattct aaggagtagg gatgtgagta gcggaacgtt atcgatgccg gtggtttcag
      121 agaggtggga tgtcgaacgc atccgtgccg atttccccgt cctgcatcag accatcaacg
      181 gcaagccgct gatctatctc gacaactccg cctcatctca agttccgcaa gtagttatcg
      241 atcgtggcag catctacatc gaacaagagc actcaaacat tcatcgcggc gttcattatc
      301 tgagccagaa agcgaccacc gcctacgaag gcgcgcgtga aaaggtgaaa cgcttcatca
      361 acgcgcgtga cgtgcgtgag tgcatcttcg tgcgcggcac gaccgaagga atcaatctcg
      421 tgatgcacgg ctatgggcgc aagttcatca acgccggcga cgagatcatc atctccgcga
      481 tggagcatca ctccaacatc gtgccctggc agatgctctg cgaagagaag ggcgcacgcc
      541 tgcgcgtgat tccgatgaac gacgcgggcg agttgctgct cgacgagtac gacggacttc
      601 tgaacgagcg cacaaagatg gtagctgtca tgcacgtgtc caacgcgctc ggcacgatca
      661 acccgatcaa ggagatgatc gcgcaggcgc acaagtatgg cgtgccggtg ttgatcgacg
      721 gtgcacaagc cgcgccgcac atgcccgtcg acgtgcagga tctcgattgc gacttctacg
      781 tgttttccgg tcacaagatg ttcgcgccaa ccggcagcgg cgtcgtttac ggcaaactcg
      841 agatactcga aacgatgaac ccgtttcagg gtggcggcga catgatcaag accgtcactt
      901 tcgagaagag catttacgcg gatccaccga cccggttcga ggcgggcact ccggctatcg
      961 catcgcaaat cggactgggc gccgccatcg attatctgaa cagcatcggc agagaacgtg
     1021 cggccgctta cgaagcagag ttgctgcgct acgcgaccga gcgcgtctcg gcgattgaag
     1081 gagtgcggat catcggcacc gcaaaacaca aggctagcgt gctgtcgttt gtgatagacg
     1141 acattcaccc gcacgacatc ggcacgatcc tggaccagga aggcatcgcg gtgcgcgctg
     1201 gtcatcattg cgcgcagccg gtgatgcaac gattcaacgt gcccgccacg gcgcgcgcgt
     1261 cgtttgcttt ttacaacacc agggaagagg tcgacgtgct cgcgcgcacg atcgaacgcg
     1321 tcattgagat atttagttaa gcgaaatgtc agaactaagc gaactctacc agcaggtaat
     1381 cctcgaccat aacaagaagc cgcgaaactt tcgcaagctc gaaaacgcga accataccgc
     1441 cgagggttac aacccactct gcggcgatca tctgaccgtc tacctcgctc tcgaaaatga
     1501 tttagtgaag gaagtcggtt ttgaaggctc gggctgcgcg atctcgaaag ccgccgcgag
     1561 catgatgacg caggcagtca aaggcaagag caagcaacaa gctgaagacc tgttccagga
     1621 gtttcattcg atggtgacgg gcgagctcaa cgaggaaatg gaagagaaca gcctcggcaa
     1681 cctgaagatc tttgcgggag tgcgtgaatt cccggtgcgc gtcaaatgcg ccaccctacc
     1741 ctggcacacc ctccacgccg cactcaacaa aaaagaccaa gtctcaacgg aatagtattt
     1801 agccgcgttt atctgcggct aatatttgtg gggcaaagtc aggactgaaa ctgtggctga
     1861 ctcagattca atcgagccga aaggttgaac ccgtggcgct cttcagttcc aatcctgact
     1921 agtgggatcg atgtctcaaa gaggggcaat tcaagctttt accagtctct tgagcacggg
     1981 tcaataaccg tttttgtgaa tgacagaatt ctggtcaagc tgatgcgaga tcggtcacat
     2041 cgcctgacca gaatcactta cttcaacgtg atatttattg cccgcaatac cccgtcaccc
     2101 atgtccacct gcaactggta agtcccaggc gtcaggcccg cggtgctgag gttgaaaaca
     2161 tactcaccat tttcaaagcg gaacaggttg ccggttgaag ctgcgcctga tgactgcacg
     2221 tcaaccgcgt cgcccactac attgtcactt atcttgtata ccagcagcct gatgaccgca
     2281 tcggttatt
//
LOCUS       DASTEY010000631         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1398813, whole genome shotgun sequence.
ACCESSION   DASTEY010000631 DASTEY010000000
VERSION     DASTEY010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398813"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VFO72_11005"
     CDS             <1..1106
                     /locus_tag="VFO72_11005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9479866.1"
                     /translation="IVILGLSITSSWGNGHATTFRALMRALSTRGHSVCFLERDMPWY
                     AENRDLPNPPFGLTQLYKTVAELKREFSPRVRNADLCIVGSYVPDGVEIGEWVIRTCR
                     GVKAFYDIDTPVTLAKLASGAAEYLTPRLIRHYDLYLSFTGGPVLRKIERELGSPMAR
                     ALYCSVDPKLYFPESRKLRWDLGYMGTYSVDRQPSLDLLLIEPARRLRMLKTVVAGPM
                     YPRELSWPANVKRIEHLEPAAHRRFYNSQRFTLNLTRADMRAAGYSPSVRLFEAAACA
                     TPIISDYWPGLETFFEPNKAILTASSPEDVIGHLKLISVSDRRAIGERARLRVLAEHT
                     SERRAIELEEYVSEVRQTSTDTPADAQHAGAIA"
     gene            1103..1702
                     /locus_tag="VFO72_11010"
     CDS             1103..1702
                     /locus_tag="VFO72_11010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9479867.1"
                     /translation="MTTFFLVRHGSCCGLGETLWGRTDGVRLNKEGKAEALRLAERFT
                     DVKLDAIYSSPLERALETAEAIARVAKLEVKQSPAFNEIDFGEWSGRSFEVLAAAEHW
                     RRFNSRRSVTKIPGGESFLDVQARVVAELERLSQQHAGGRIAIVSHADVIKAAVGYVA
                     GTPIDLLQRFEISPCSVSIIATDEHETRMLALNSRCELM"
     gene            1704..>2217
                     /locus_tag="VFO72_11015"
     CDS             1704..>2217
                     /locus_tag="VFO72_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479868.1"
                     /translation="MKIVFFGLTISSSWGNGHATLLRGLFKALTARGHEIVFFERDVP
                     YYAAHRDLVESPHIKLRLYRDWHDNLPTARKAVAEADVAVVTSYCPDGIDATELVASS
                     NVSLRVFYDLDTPVTLEALQNGEQLSYIGPRKLVDFDLVLSYTGGAALTALEAQLGAR
                     RVVPLFGSVDP"
BASE COUNT          482 a          663 c          626 g          446 t
ORIGIN      
        1 acatcgttat cctcggatta tcgatcacct cgtcgtgggg taacgggcac gcgacaacgt
       61 ttcgcgcgct gatgcgggcg ttgagcacgc gaggtcatag cgtctgtttc ctcgaacgcg
      121 acatgccctg gtacgcggag aatcgcgatc tcccaaatcc gcctttcgga ttgacacaac
      181 tctacaagac agtggcagag ttgaagcgcg agttctcgcc gcgagtgcgg aatgctgatc
      241 tctgtatagt gggttcgtac gtgcccgatg gcgtcgagat cggcgagtgg gtcatccgca
      301 cttgccgcgg cgtcaaggcg ttctacgaca tcgatacacc cgtgacgctg gcgaagcttg
      361 caagcggcgc tgctgaatat ctgacgccgc gcctcatccg tcattacgat ctttatctgt
      421 ctttcacggg tggaccggtg ctgcgcaaaa tcgaacgcga actcggctca ccaatggcgc
      481 gcgcgctcta ctgctccgtc gaccccaagc tctattttcc ggaaagtcgt aagctgaggt
      541 gggacctggg ctacatgggc acttacagcg tcgatcgcca accatcgttg gacctattgt
      601 tgatcgaacc tgcaagaaga ctgcgcatgt tgaagacagt cgttgccggg ccgatgtacc
      661 cacgcgaact gtcgtggccc gccaatgtca aacggatcga gcatctcgaa ccggcagcgc
      721 accgtcgctt ctacaattcg caacgcttca cgctcaacct gacgcgcgcc gatatgcgcg
      781 ccgcaggtta ttcgccgagc gtgcgactgt tcgaagcggc cgcgtgcgcg acgccgatta
      841 tcagtgacta ctggccggga ctggaaacat ttttcgaacc caacaaagcg atcctgacgg
      901 catcttctcc agaagacgtt atcgggcatt tgaaattaat ttccgtgagc gatcgacgcg
      961 cgattggcga acgcgcgcgc ctgcgtgtgc tggcggaaca tacctccgag cgacgcgcga
     1021 tcgaactcga agagtacgtc tcagaagtta ggcaaacatc caccgataca cctgccgatg
     1081 cgcaacacgc aggagcaatc gcatgacgac tttcttctta gtcagacacg gatcgtgctg
     1141 cggactcggc gagacgttgt ggggacggac ggacggagtg cgcctgaata aagaaggaaa
     1201 agcagaagcg ctgagactag ccgaacgatt cacagacgtg aaactggacg cgatctacag
     1261 tagtccactc gagcgtgcgc tcgaaaccgc ggaagcaatc gcgcgtgttg cgaaactcga
     1321 agtcaaacaa agccccgcat tcaacgaaat cgatttcggt gagtggagtg gcaggtcctt
     1381 cgaagtgctg gccgccgctg aacactggcg acgcttcaac agccggcgca gcgttacgaa
     1441 aattcctgga ggcgagtcgt ttttagacgt gcaagcgcga gttgtcgccg aactcgaacg
     1501 gctctcgcag caacacgccg gcgggcgcat cgcaattgtg tcgcacgctg acgtgatcaa
     1561 agctgccgtc gggtatgttg cgggcacgcc gatcgacctc cttcagcgtt tcgagatttc
     1621 gccatgctcc gtcagcatta tcgccaccga cgaacatgaa acacgaatgc tggctctaaa
     1681 cagccgctgc gaactgatgt gaaatgaaga tagttttctt cggtctaacg atcagctctt
     1741 cgtggggaaa cggccacgcg acgctgctgc gcggtctttt caaggcgctc accgcgcgcg
     1801 gtcacgagat cgtattcttc gaacgcgacg tgccgtacta cgccgcgcac cgcgatctgg
     1861 tcgagtcgcc gcacatcaag ctacgtctct atcgagactg gcatgacaac cttccaacag
     1921 ctcgcaaggc tgtggccgaa gcggacgtcg cagtagtcac ctcttactgt cccgacggca
     1981 tcgacgccac cgaactagtc gcttcctcga acgtcagctt gcgcgtgttc tacgatctcg
     2041 acacgcccgt caccctcgaa gcacttcaga acggcgaaca actcagttac atcggcccac
     2101 gcaaactggt cgacttcgat ctcgtcctga gttacaccgg cggagcagca ctgaccgcgc
     2161 tcgaagctca actcggtgca cgtcgcgtcg tgccgttgtt tggcagcgtc gatcccg
//
LOCUS       DASTEY010000632         4322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_327498, whole genome shotgun sequence.
ACCESSION   DASTEY010000632 DASTEY010000000
VERSION     DASTEY010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327498"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VFO72_11020"
     CDS             <1..842
                     /locus_tag="VFO72_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HET9479869.1"
                     /translation="IPDEERHYSTIAASSIGIAIHHLSADKYQLFESELSAPEPFLVS
                     PFSGQFNDLLRLMTGHGRVALTGYDGDALMNETRCSYLGRLKARIKTKREEISYPAWI
                     DESFAKRIELKDRVREVWSEPRVNNKRNSETMRALQSKVWAPLFEGYDPGATRLPLEV
                     RHPFMDVRLVEYLLSIPVKPWRLNKNILRRAMKGRLPDSIIDRPKTPLAGDPALQLSW
                     RAGVRWRDKFEVTPQLTRFVNLSRGRSTAEETPNELWANLRLFAFSHWLTHSLPLDRR
                     MVA"
     gene            860..1036
                     /locus_tag="VFO72_11025"
     CDS             860..1036
                     /locus_tag="VFO72_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479870.1"
                     /translation="MARTPETDQPRNPDQTKKPYHKPEVIVYGNIREITRNTGPKGAP
                     DNGGGAAAGPKTGQ"
     gene            1046..2023
                     /locus_tag="VFO72_11030"
     CDS             1046..2023
                     /locus_tag="VFO72_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479871.1"
                     /translation="MLYGCSIYGLGVLANRPIPTVPYSPITTADVRVSFGELPDWLDR
                     LQPHRVEETYVADYTDNAGNPAFVFSKLDGGKFYRFSYADNTEFVVDRDGREIWTRWD
                     APLTLEDTATYLLGPVMGFVLLLRGLMCLHASAIVVDGGAIALVGPAGSGKSTTAAAF
                     SARGFSVLAEDVVTLDDRADRFFVRPAYPCIRLWPASAATLYGSRSALPPLTPNWDKC
                     YLDLTQQSGQFESESQPLAAIYLLGERSDDPEAPFVESVDRADGLISLIANTYGTKLI
                     NKQMRAREFDLLSRVLTHVPLRRVTPHTDPSRLAELCDRLLSDFERLHV"
     gene            2016..2975
                     /locus_tag="VFO72_11035"
     CDS             2016..2975
                     /locus_tag="VFO72_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9479872.1"
                     /translation="MYSLHFYGEMLANAPRIAPYVEALRRTVKPGSVVLDLGCGPGVF
                     ALLACHFGARRVYAIEPDNVINIAREAAVANGFANRIEFFQNSSTEVTLPEHATIIIS
                     DLRGVLPWFQRHIPSLIDARGRLLAPGGVLIPRRDVLWAAPVEARERYEEIVRPWQHD
                     RFNLDLSAGLTRITNAWRKTRIKPDELLSQPVCWATLDYHTVDTPDVQAEISWRAVRS
                     GIAYGFALWFDTELVDGVGFSNHPSAPELIYGNGFFPFPRPVEVSEGERIAVSLRADL
                     VQDDYVWTWATDFRDSKTNFRQSTFYSVALSPEQLRKKHAQRS"
     gene            2959..4158
                     /locus_tag="VFO72_11040"
     CDS             2959..4158
                     /locus_tag="VFO72_11040"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9479873.1"
                     /translation="MLNVLRRPEHEILFLIARRDLDPEQRAIARELLSQVQDWAYLLA
                     TASTQGLLPLLHKHLQLIGADVVPIDKRSRLKREAVNNSQNVLHLLGKQFKLYRLLKA
                     NSIPVAIFKGSVLAQMAYGEISLRQAGDIDVLISRSHFRQARQLLESLGYQMTPSLTD
                     VQLTSHLTSHCEIQFVRDDWLTVVDLHWAMAPKNFVFKVETDELLSRLQAVRVGGTEI
                     ETLATEDLILYLSMHGAKHLWRALEWISSLGELLRPAESIAWDMVIERAEKARATRML
                     ALGLRLAETVSGVHAPPEVLRVVDGDESSKRMARETLSRIFNVSAAAVSSETNLYNLK
                     VMDRKRDALLSALRAIFVPTFTDWSALNLPPSLHPLYYAYRPLRLSKSYGTSIWRKRS
                     SRGATCL"
BASE COUNT          934 a         1189 c         1205 g          994 t
ORIGIN      
        1 tgattcccga cgaagagcgc cactattcaa cgattgccgc ctcgagcatt ggtatcgcta
       61 ttcatcacct tagcgctgac aagtaccagt tattcgaaag cgagctaagc gcgccggagc
      121 cgtttctcgt atcgcctttc agtggacagt tcaacgatct gttgcggctg atgaccgggc
      181 acggacgtgt tgccttgacc gggtatgacg gtgacgcgtt gatgaacgaa acgcggtgtt
      241 cctacctcgg caggttgaag gcacggataa aaaccaaacg cgaagagatc tcttaccccg
      301 cgtggattga cgagtcgttt gcaaagcgaa tcgagttgaa agatcgcgtg cgcgaggtct
      361 ggtcggaacc acgcgttaat aacaagcgca actcggaaac aatgcgcgcg ctccaatcga
      421 aagtgtgggc gccgttgttt gaaggctacg accccggcgc cactcgtctg ccgctcgaag
      481 tgcgtcaccc attcatggac gtacgtctgg tcgagtactt gttatcaatt cccgtgaaac
      541 cgtggcgcct gaacaaaaac atcctgcggc gagcgatgaa gggtcgctta cctgactcga
      601 taatcgatcg tccaaagaca ccactggccg gcgatccggc tcttcaattg tcttggcgtg
      661 ctggtgtaag atggcgcgac aaatttgagg tcacgccgca gcttacgcgt tttgttaacc
      721 taagtcgcgg gcgatcgacg gcggaggaaa ctcccaacga actttgggcc aatctccggt
      781 tgtttgcatt cagtcattgg ctcacacatt ctctgccgct cgatcgacga atggtcgcct
      841 aattcaactg gagaaaagca tggcacggac accagaaacc gatcagccac gtaatcccga
      901 ccaaaccaaa aagccctacc acaaaccgga agtcatcgtt tacggaaaca ttcgcgagat
      961 cacccgcaac accggcccga agggagctcc cgacaacggc ggcggcgcag ccgctggacc
     1021 aaagactgga cagtgatgtc gtaacatgct ttatggctgt tcaatctacg gtctgggcgt
     1081 attagctaac agaccaatcc ccaccgttcc ctattcgcct attacaaccg ccgacgttcg
     1141 tgtctcgttc ggagagttgc ccgattggct tgaccgttta caacctcacc gggtcgagga
     1201 aacgtacgtt gccgattaca cggacaatgc cggcaatccg gcgtttgtgt tttccaaact
     1261 ggacggcggc aagttctacc ggttttctta cgccgacaac actgaattcg tcgtggaccg
     1321 tgacgggcgt gagatttgga caaggtggga cgcgccgttg acgctcgaag acactgctac
     1381 gtatctactc ggtccggtga tgggattcgt gttgctgctg cgcgggctca tgtgcttgca
     1441 cgccagcgca atcgttgtcg atggtggagc aatcgcttta gttggcccgg ccggatccgg
     1501 aaaatcgaca acggcggcag cgttttccgc acgggggttc agcgtcctgg cggaagacgt
     1561 cgtgacgctc gacgatcgcg cggatcgttt ctttgttcgc cctgcttatc cgtgtattcg
     1621 gctgtggccc gcttctgccg caactcttta cggatctcgc tcggcattgc cgccactcac
     1681 accgaactgg gacaagtgct acctcgactt aacgcagcag tcgggacaat tcgaaagtga
     1741 gtctcaacca ctcgccgcaa tttatctgct tggcgagcgc agcgatgatc cagaggcgcc
     1801 gtttgtggag tcagtcgatc gagccgatgg tttgatttcg ttgattgcca atacgtatgg
     1861 cacaaagcta attaacaaac agatgcgcgc ccgcgagttt gatctgttga gtcgtgtctt
     1921 aacgcatgtg ccgctgcggc gagtgacacc gcataccgat cccagccggc tcgcagaact
     1981 ttgcgaccgt ctgttgtctg atttcgaacg tctgcatgta tagcctccat ttttacggtg
     2041 aaatgcttgc gaatgcgccg cggatagcgc cttacgtcga ggcgctgcgc agaacagtga
     2101 agccgggttc agtggtgctg gatctcggtt gtggtccagg cgtgtttgcg ttgctggcgt
     2161 gccacttcgg tgcgcggcgt gtttacgcga tcgagcccga taacgtgatc aatatcgcgc
     2221 gtgaagcggc cgtggctaat gggtttgcca atcgaattga gttcttccaa aacagttcga
     2281 cggaagtcac gttgccggaa cacgcaacca taatcatttc tgacttgcgc ggcgtcttgc
     2341 cgtggtttca acgacacatt ccgtcgctta tcgacgcgag agggcgactg ctggccccgg
     2401 gcggcgtgtt gattccgcgc cgtgacgtct tgtgggcggc gcccgtcgaa gccagggaac
     2461 gatacgagga gatcgttcgg ccttggcaac acgatcggtt caacttagat ctctccgccg
     2521 gtctcacgcg aattacgaac gcgtggcgca agacgcgaat caaacccgat gagttgctga
     2581 gtcaacccgt gtgttgggct actttggact accatacggt cgatacaccg gatgttcagg
     2641 ctgaaatttc ctggcgcgca gtgcgatccg gtatcgcata tggatttgcg ctgtggtttg
     2701 atacggaact ggtggacggt gttggtttct caaatcaccc ttctgctccg gaattgatct
     2761 acggcaatgg attctttcca tttccacgtc cggtggaagt cagtgaaggt gaacgaatcg
     2821 cggtgagcct gcgcgcagat ttagtgcagg acgattacgt atggacctgg gccacggatt
     2881 ttagagattc aaaaaccaat tttaggcaat cgacgtttta cagtgtggcg ctctcgccgg
     2941 agcaactgcg gaagaaacat gctcaacgtt cttagacggc cggaacacga gattctgttt
     3001 ttgatcgctc ggcgcgattt agacccggag caacgcgcga tcgctcgcga gttactgtcg
     3061 caagttcagg actgggccta cctgcttgca acggcaagca ctcagggctt gctgccactg
     3121 cttcacaaac acctacaact tatcggcgca gacgttgttc cgatcgacaa gagatctcgg
     3181 ctcaagcgtg aagctgttaa taattcgcag aacgtcttgc acctgctggg caaacagttc
     3241 aagctctacc gtttgttaaa agcgaacagc attccagtgg cgattttcaa aggctcggtc
     3301 ctggcgcaaa tggcttatgg cgagatctcg ctgcgtcagg cgggtgacat cgatgtgctg
     3361 atttcccgat cgcacttccg tcaagcaaga cagttgctgg agtcgctagg ctatcagatg
     3421 acaccgtcgt taactgacgt tcaactaact tcgcacctca cctctcattg cgagattcag
     3481 tttgtacgcg acgattggct caccgtcgtg gatcttcact gggcgatggc gccaaaaaac
     3541 ttcgtcttca aagtagaaac agatgagctg ctgtcgcgtt tgcaagctgt aagagtcggt
     3601 ggcacagaga tcgaaacgct tgcgactgag gatctgatct tgtacttatc gatgcacggc
     3661 gcaaaacatc tctggcgtgc gctcgaatgg atcagttcac ttggtgaact actgcgcccg
     3721 gccgaatcaa tcgcctggga catggtaatc gaacgcgccg agaaagcacg cgccacacga
     3781 atgttggccc tgggtctgcg tttggcagag acagtttcag gcgtgcatgc tccccccgag
     3841 gtattacgag tggtggacgg tgacgagtcg agcaagcgaa tggctcgaga gacactatcg
     3901 cggatcttta acgtttctgc cgccgcggtt tccagtgaga caaatctcta caacctgaag
     3961 gtcatggacc gcaaacgcga cgcactcctt tccgccctgc gcgcgatctt cgtgccgact
     4021 ttcaccgact ggtcagcgct gaacctgccg ccgtcattgc atccgctcta ctacgcctat
     4081 cgcccgctac gactctcgaa gtcctacggc acttccatct ggcgaaaacg aagctcgaga
     4141 ggcgctacat gcttgtgact ctcaaacctc ctttgaagtg ggccggtggc aaacgatggc
     4201 tggtgccgca tctcaaaccg atctgggagc ggtactcaga tcttcgttac gtcgaaccgt
     4261 tttgcggcgg cctcgcagtc gtgttgggtc tgaaaccaac gcgcgcgctg ctcaacgaca
     4321 tc
//
LOCUS       DASTEY010000633         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_821861, whole genome shotgun sequence.
ACCESSION   DASTEY010000633 DASTEY010000000
VERSION     DASTEY010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821861"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFO72_11045"
     CDS             <1..213
                     /locus_tag="VFO72_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET9479874.1"
                     /translation="AHIHSGAVDKAGPPVLTLKAPATGSSKECATLEKDKVLDIIKNP
                     ANYYVNVHNAEFPDGAVRGQLAKKGS"
     gene            complement(259..1953)
                     /locus_tag="VFO72_11050"
     CDS             complement(259..1953)
                     /locus_tag="VFO72_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET9479875.1"
                     /translation="MTSNLALIKKPETNGNGLVASVPPPSPKQPVEPLTEEKVNELLS
                     GRGLRGWLRAARVARVLGLFSLYLFLDTYDVRADFNRRAVTRLRDLARKKGRSAQFKA
                     WFNAQLYAALDRFIRVLRYAIFRGAEGSERKQARLEKQAVWMRESLIDLGPTFIKIGQ
                     ALGTRADLLPLAYVKELATLQDQVPAFPTDEAFAIVESELGRSLHEAYAEIDSEPIAA
                     ASLGQVYRARLATGEEVAVKVQRPNLESTISFDVAILFRLVKLTNRFFPRANENADWE
                     GMLREFHSTIFEEMDYVKEGRNADRFRYNFRTWRAIRVPKIYWSHTNTRVLTLDFIRG
                     TKVVDLEGLRANRISPVKVNRLLIRTYLKQLLEDGFFHADPHPGNLLVMDSGHLAFFD
                     FGMVGRISPKLQSQMIDAFFHVVARDVKGLGQDIINLNFLKPGVDPETVRPVVESLFT
                     HYLNLRLGEVNFKELTYDLAEVIYEYPFRLPSNFTYIMRALMTLEGIGVVTDPGFSFF
                     ETAKPFAREFMLRREGRMFRKLIFDKLTGRENGESQIAWSRVWKLTKMAARMYLEK"
     gene            complement(1966..>2615)
                     /gene="ribF"
                     /locus_tag="VFO72_11055"
     CDS             complement(1966..>2615)
                     /gene="ribF"
                     /locus_tag="VFO72_11055"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HET9479876.1"
                     /translation="EEFLKDVVVDRLHAKEVYLGKGFAFGHNREGNIELLKRMGSELG
                     FVAGEVPEVKLRGCRVSSSKIRQLLASGNVNLARRMLGRPYGVEGRVERGSERGHKLG
                     FPTANLHPHNRVIPRNGVYVTGTLIEGQWRRSVTNIGVRPTFADGTEPSVETFVMNWD
                     GDLYGDVVRVRFLYRLRDERKFSSIEQLKTQIARDVQRANSYFDRAGTKHMLSVI"
BASE COUNT          598 a          782 c          678 g          557 t
ORIGIN      
        1 gctcacatac attcaggcgc ggtggacaag gctggcccac cggtcctgac gctgaaggcc
       61 cccgccaccg gctcgtccaa agagtgcgcc acgctcgaga aagataaggt cctggacatc
      121 atcaagaacc cggcaaacta ctacgtgaat gtccacaatg cggaattccc ggacggcgcg
      181 gtgcgcggcc agctagcgaa gaaagggtct taaattcaac agatggaata ggtcctattt
      241 gtcttatagg acctattctt acttctccaa atacattcgc gcggccatct tggtcagctt
      301 ccaaaccctg gaccacgcaa tttgactctc gccgttctcg cggccggtca gtttgtcgaa
      361 gatcagcttc cgaaacatcc ggccttcgcg tctgagcatg aactccctcg caaacggttt
      421 tgccgtctca aagaagctga acccaggatc ggtgaccacg ccgataccct cgagcgtcat
      481 cagcgcgcgc atgatgtaag taaagtttga cggcaagcga aaaggatact cgtaaatcac
      541 ttccgccaga tcgtaagtca actccttgaa gttcacttct ccgagcctga gattgagata
      601 gtgcgtgaag agactctcca ccaccggacg cactgtctcc ggatcgactc cgggcttgag
      661 gaagttcaga ttgatgatgt cctggcccaa ccccttcacg tcacgcgcaa ccacgtggaa
      721 gaaggcatcg atcatctgcg actgaagctt cggactgatc cgtcccacca ttccgaaatc
      781 gaaaaacgca agatgcccgc tatccatcac gagcagattt cccggatgtg gatcggcgtg
      841 gaagaacccg tcctcgagta gctgcttgag ataagtacga atcagcagcc ggttaacctt
      901 aacgggcgaa atgcgatttg cgcgcagacc ttcaagatca accacctttg tgccgcgaat
      961 aaaatcgagc gtgagcacgc gcgtgtttgt atgcgaccag tagatctttg ggacacgaat
     1021 cgcgcgccag gtgcggaagt tgtagcgaaa cctgtcggca ttacgtcctt ccttgacgta
     1081 atccatctcc tcaaagatcg tcgagtgaaa ctcacgcagc attccttccc aatcggcgtt
     1141 ctcattcgca cgcggaaaga accgattcgt cagcttcacg aggcgaaaca ggatcgcgac
     1201 atcgaacgag atagttgatt cgaggttcgg acgctgcacc ttgaccgcga cttcttcgcc
     1261 agtcgccagg cgtgcacgat agacctgtcc gagtgacgca gcagcgatcg gctcggagtc
     1321 aatctccgcg tacgcttcat gaagactgcg acccagttcc gattcaacga ttgcaaaagc
     1381 ttcgtcagtg gggaatgcgg gcacctggtc ctgaagcgta gccaattctt tgacgtatgc
     1441 cagcggcaac agatcggcgc gcgtgccgag ggcctgaccg atcttgatga acgttggacc
     1501 aagatcgatc agcgactcac gcatccacac cgcctgcttt tcgagacgcg cctgttttcg
     1561 ctccgagcct tcagcgccac gaaagatcgc gtaacgcaag acgcgaatga accgatccaa
     1621 cgcggcgtag agttgtgcgt tgaaccaggc cttgaactgc gccgagcgtc cctttttcct
     1681 cgcgagatct cgcagtcgcg taactgcgcg gcggttgaag tccgcgcgca cgtcgtaggt
     1741 atcgagaaac aggtaaagcg agaagagtcc caacacacgc gcgactcgcg ccgcacgcaa
     1801 ccagccccgc aagccgcgcc cggagagaag ctcattgacc ttctcttccg ttaacggttc
     1861 gaccggctgt ttcggcgacg gtggcggcac gctggccacc aggccatttc cattcgtttc
     1921 cggtttctta attagtgcta gattcgaagt cattttaata acgttttata tcactgacag
     1981 catgtgctta gtgccggctc ggtcgaagta actgttcgct cgctgcacgt ctctcgcaat
     2041 ctgggttttc aactgctcga tcgagctgaa cttccgctcg tcgcgaagac gatacagaaa
     2101 acgcacgcgc accacatcgc cgtaaagatc gccgtcccag ttcattacga aggtctcaac
     2161 tgaaggttcc gtgccatccg cgaacgtcgg tcgcacgcct atatttgtaa cactccgacg
     2221 ccattgtcct tcaatcaacg tcccggtcac atacacaccg ttcctgggta tgacgcggtt
     2281 gtgtgggtgg aggttggccg tgggaaaacc gagcttgtgg cccctttccg agcctcgctc
     2341 aacacgtcct tcgacgccgt aaggacgacc caacatgcgt cgcgcgaggt tgacgttgcc
     2401 gctggcgagc aattgccgga tcttactcga gctcacgcgg caaccgcgca gtttaacctc
     2461 cggtacttca cccgcaacga agccgagctc gctgcccatg cgtttcaaca actcgatatt
     2521 gccttcgcgg ttgtggccga aagcgaagcc cttgccgaga taaacttcct tcgcgtgcag
     2581 acgatctaca actacgtcct tgagaaattc ctcgg
//
LOCUS       DASTEY010000634         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1563049, whole genome shotgun sequence.
ACCESSION   DASTEY010000634 DASTEY010000000
VERSION     DASTEY010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563049"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFO72_11060"
     CDS             complement(<1..455)
                     /locus_tag="VFO72_11060"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9479877.1"
                     /translation="MFSLLFLAFLLFIPQGGSIKGKVVADIPDQLKTLSGVVVFLSGD
                     RLRDQKLQTVTDSEGEFTFNGLIAGDYTVSVEFMGFRKYEQKLSIQIDATVEHDILLQ
                     PVPLSESVTVNDDRTDAVKTESTTPSVITTQNLRDAPLIDQKFQDALPLL"
     gene            621..1229
                     /locus_tag="VFO72_11065"
     CDS             621..1229
                     /locus_tag="VFO72_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479878.1"
                     /translation="MALPPLTFAGADGPSATGSFQFALEDGNPRFLEFNARAQNNGRI
                     VGEMSFSDPNLSLVADPDAPGNSPATGASIRASFDCLKIDGNRAVMSGVIFESNVLTA
                     IGLRVLLVVEDNGEGINQPTPDKLTWGVYQNPASGWTPKDSERDDDNGATLSWLATDA
                     ERPDDVGIPSNPGRTIGCQSFPLSAYSFADIVHGGGNVQVQP"
     gene            complement(1357..>2133)
                     /locus_tag="VFO72_11070"
     CDS             complement(1357..>2133)
                     /locus_tag="VFO72_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9479879.1"
                     /translation="IEWGSASSPIIWKDLVILQCDTQTDSFIVALNTNTGETVWKTDR
                     DEPPSWGTPTVAITSKGDELIANASNFIRGYDPRTGKELWRLGKSSKITAPTPVFADD
                     ILVVASGRGPQRPIFVVKAGARGDLTLPDGKTSSDAIIWSRTGRGSYMPTPLIYKGNL
                     YVLGNNGTFDAYNLKTGDELYRQRLPLVGNGFSASPVAADGKIYLSNEDGEILVISAG
                     EKFAHVATNSMGELLMATPALSEGVMYVRTAQSVFAVGRK"
BASE COUNT          458 a          593 c          605 g          477 t
ORIGIN      
        1 agcagcggca gcgcatcctg aaacttctga tcgatcagcg gcgcatcacg caggttttga
       61 gttgtgataa cggaaggcgt ggttgattca gtcttgaccg catctgtgcg atcgtcgttg
      121 acagtgactg actcgctcag cggcactggc tgcaacagga tgtcgtgttc gactgtggca
      181 tcgatctgaa tactcagttt ctgttcgtac ttcctgaagc ccatgaactc gacggaaaca
      241 gtgtaatcac cggcgatgag cccgttgaag gtaaactcgc cttccgaatc ggtgaccgtc
      301 tgaagttttt gatcgcgcag tcggtcgccg cttagaaaaa ccacgacgcc ggaaagcgtt
      361 ttaagctgat cgggaatatc agcaaccacc tttcctttaa tgcttccgcc ttggggaata
      421 aaaagcagga atgccagaaa caataagcta aacatgcgaa attcctagca cagttgagtg
      481 ccgataacca agtacggcgc ctcgattgac ttctgtgccg gtttgacgtt ataagccgtt
      541 cactaagatc ctggcccaga aggagtcccc caatgaaaag cctttcttgt gtagcggttt
      601 tctgcctcct gttggtgtgc gtggcgctcc cgcccctgac ctttgccggc gccgacggac
      661 catcagctac cggttctttt caatttgctc tcgaagacgg taatccaaga tttctcgagt
      721 tcaacgctcg cgcacagaac aacggccgca ttgtcggcga gatgagtttc agcgatccca
      781 acctgtcgtt agtggccgac ccggatgcac cgggcaactc acctgctacc ggcgcctcga
      841 tccgcgcgtc gttcgattgt ttgaagatcg acggaaaccg cgcggtgatg agcggcgtca
      901 tctttgagtc aaatgtgcta acggcgatcg gactgcgtgt gttgttggtg gtggaggaca
      961 acggcgaagg cattaaccaa ccgacgccgg acaagctcac gtggggcgtt tatcagaatc
     1021 cggcgagcgg atggacacca aaagattccg aacgcgacga tgacaacggc gccactttga
     1081 gctggttggc gacagacgcc gaacgcccgg acgacgttgg catcccctcg aatccaggca
     1141 ggaccatcgg ctgtcagagt tttccgctgt ctgcgtactc tttcgccgac atcgtgcacg
     1201 gtgggggaaa cgtgcaggtc cagccgtaat acgcaggctc aaacccggga ccgcaggcgt
     1261 ctcgcctgcc tggcagcgaa gaaggcgacc tccgaagttt gccggcgaga cgtctgcggt
     1321 cccagcaaca aggttgtcaa actgaaattc gtgctactac ttccgcccga cagcgaacac
     1381 actttgcgcg gtgcggacgt acatcacgcc ttctgacagc gcgggcgtcg ccatcaaaag
     1441 ctcgcccatt gaattggtgg cgacgtgcgc gaacttctcc cccgcggaaa ttaccaaaat
     1501 ctctccgtct tcattcgaca agtaaatctt tccatctgcg gcaacaggcg aagcactgaa
     1561 gccgttgccc acgagcggaa gtcgttgccg gtagagttcg tcgcctgtct tcaggttgta
     1621 cgcgtcgaaa gtgccgttgt tgccgagtac gtaaagattg cctttgtaga ttagcggcgt
     1681 tggcatgtaa gagccgcgtc cggtgcgact ccagatgatc gcatcgctgc tggtctttcc
     1741 gtcgggcaac gtcagatcgc cacgagctcc ggctttgacg acgaagatcg ggcgctgtgg
     1801 tccacgcccg ctggcaacga cgagaatgtc gtctgcgaag accggcgtcg gcgcagtgat
     1861 cttcgagctc ttgccgagac gccacaactc tttgccggtg cgtggatcgt aaccgcgaat
     1921 gaagttcgag gcgttcgcga tcagttcgtc accttttgac gtgattgcga cggtcggtgt
     1981 gccccacgac ggaggctcat cacgatcggt tttccaaact gtctcgccgg tgttggtgtt
     2041 aagtgcgacg atgaaagaat cggtttgcgt gtcgcattga agaatgacga gatccttcca
     2101 gatgattggt gaactcgccg aaccccattc gat
//
LOCUS       DASTEY010000635         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_97025, whole genome shotgun sequence.
ACCESSION   DASTEY010000635 DASTEY010000000
VERSION     DASTEY010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97025"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFO72_11075"
     CDS             complement(<1..372)
                     /locus_tag="VFO72_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479880.1"
                     /translation="MSTCTRLLFLTLLLSFSINAQQPSPSPSPSPSPSATPAADASPA
                     PRDPMSTPTFNGLRLRSIGPAFTSGRVSGFAVDPKNPARYFVGVASGGVWKTVNAGTT
                     WTPVFDNEASYSIGAIVLDPKN"
     gene            complement(432..1118)
                     /locus_tag="VFO72_11080"
     CDS             complement(432..1118)
                     /locus_tag="VFO72_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9479881.1"
                     /translation="MGELVQFKANGRTGDGYLALPKQGSGPGVLVIQEWWGLVDHIKD
                     VCERFAEEGFVALAPDLYHGKATRSPDEAGKLMMALRIDDAEKDLSGAVRYLLDHDAT
                     IGDKAGVVGFCMGGALSLYTATKNADVGACVVFYGGHPNVKPDLPNLQAPVLGLYAER
                     DGFVTPESVHKLEKQLKALGKQIDTIIYPQTDHAFFNDTRPEVYNANAAADAWQRTIE
                     FLRKHLSKQP"
     gene            complement(1128..1721)
                     /locus_tag="VFO72_11085"
     CDS             complement(1128..1721)
                     /locus_tag="VFO72_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HET9479882.1"
                     /translation="MQQPARLVVGITGATGTIFGVRLLQMLHGSGVETHLVVSKWAAR
                     TLAHETQYTLKDVQNLATHSHGIGDQGATISSGSFVTLGMVIAPCSMRTVAAIAHGLG
                     DNLIHRAADVVLKERRKLVLVVRESPLNEIHLENMLKLARMGVVILPPVPAFYNNPQN
                     LDDMINHITMRVIDQFDIHLDVMNRWDGVLRLPMEKE"
     gene            complement(1811..2149)
                     /gene="trxA"
                     /locus_tag="VFO72_11090"
     CDS             complement(1811..2149)
                     /gene="trxA"
                     /locus_tag="VFO72_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9479883.1"
                     /translation="MSEFVKDVNDSSFETDVLGSDKPVLVDFWAEWCAPCRMLAPTVA
                     AVAEHYGENASVVKLNVDDNPSTAQRYGIKGIPTLILFREGKEVERVVGATSKESITR
                     MVEKYVTVKA"
     gene            complement(2190..3860)
                     /gene="rpoD"
                     /locus_tag="VFO72_11095"
     CDS             complement(2190..3860)
                     /gene="rpoD"
                     /locus_tag="VFO72_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET9479884.1"
                     /translation="MNSVKGDFANLSEELTDDAVSIEDIDEVLQTLERATSSETEIEE
                     PLIEESSSAVESDEAMSGEEVELDLSAGEMDKTSDPIRVYMREMGVVPLLTREQEVSI
                     AKRIERGQKRAEKAIARSPIAVVELFRIGDELAEGKLNIRDVVAFSDQMETEEHEDRS
                     EEYLQWTIEGLNNIRQLYKRGLKEFERFAGEQKASRGKRSKKLLRYRRKLARTRLEIA
                     QEIRGLHMKEAARQRLIAAIGVVHKEVRALEREIEAASEKLKRKGLKPDKQKEYRKQV
                     AAAKKRLKEIEAEHHLSPLEIKRSHQMIAVSEAQTAQAKHELTEANLRLVVSIAKKYQ
                     NRGLQFLDLIQEGNLGLMKGVDKFDWRRGYKFSTYATWWIRQAITRAIADQARTIRIP
                     VHMIEVINKLKNTSRELVRELGREPTTEEIAKKMDTSVDKVRKARKLMQDPISLETPI
                     GENGDSQLGDLIEDKSSESPAARVITSNLREIAGDVLQTLSPREERVIRLRFGLDNGG
                     HERTLEEVGQNFNVTRERIRQIEAKALRKLRHPSRARLLKNFLEGEGQ"
     gene            complement(3920..>4311)
                     /locus_tag="VFO72_11100"
     CDS             complement(3920..>4311)
                     /locus_tag="VFO72_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9479885.1"
                     /translation="MLLDLAQAGLVVSQTGSMGGSRLATDPAKTTLLDIYQALECGGV
                     FSLHRQPPSRDCPVGVNIETVLGEVLQEVDAAVEQVLKNITIDDVVQRLKPCGTLTNV
                     KSPRLGKSLGKPLGKSNGKIAKVLETA"
BASE COUNT          873 a         1267 c         1120 g         1051 t
ORIGIN      
        1 atttttcgga tcgagcacga tcgctccgat tgagtacgac gcttcgttgt cgaaaacagg
       61 cgtccacgtt gtgccggcgt taactgtttt ccagacgccg ccggaagcga cgccgacgaa
      121 gtagcgggcg gggttttttg gatccaccgc gaaaccggag acacgtcctg acgtgaacgc
      181 tggaccaatc gaacggagac gcaaaccatt gaatgtcggc gtcgacatcg gatcgcgcgg
      241 cgccggcgat gcgtcagccg ccggtgttgc gcttgggctc ggactcggtg atggcgaagg
      301 cgatggttgt tgggcgttta tactgaaaga caaaagcagg gtcagaaaca acaaacgagt
      361 acacgtagac atagtcttcg agcgcttcct ttagattgga ctaggagaca cggataaaca
      421 caggtccgtg tttacggctg cttacttagg tgctttctta aaaactcgat agtgcgctgc
      481 catgcatccg cggcggcatt agcgttgtag acctcagggc gcgtgtcgtt gaagaacgcg
      541 tgatccgtct gcgggtagat gatcgtgtcg atctgttttc ccagagcttt caactgcttc
      601 tccaacttgt gaacggattc gggtgtgaca aacccatcgc gctccgcgta aaggccgagt
      661 accggcgcct gcaggttcgg cagatcgggt ttcacgttgg gatggccgcc atagaaaacg
      721 acacatgctc ctacgtcggc gttctttgtc gcggtgtaaa gagacagcgc gccacccatg
      781 caaaaaccca ctacgccggc tttatcgccg atagtcgcat cgtggtccaa cagatatcga
      841 actgcgccgc tgagatcctt ttccgcgtcg tcgatgcgca gggccatcat caacttgcct
      901 gcttcgtcgg gagatctcgt ggccttgccg tgatagagat cgggcgcgag ggcgacaaaa
      961 ccttcttccg caaaccgctc gcaaacgtcc ttgatgtgat ccacgagtcc ccaccactcc
     1021 tgaatcacga gcacgcctgg accactaccc tgtttcggaa gcgcaagata gccatcgcct
     1081 gttcgaccat tcgctttaaa ctggactagt tctcccacag ctcctaccta ctccttttcc
     1141 atcggcaaac gcaatacgcc gtcccaacga ttcatcacat ccaggtggat atcaaactga
     1201 tcgatcacac gcatggtgat gtggttgatc atgtcgtcga gattctgagg attgttatag
     1261 aacgccggca ccggtggcaa gatcacgacg cccattcgcg ccagcttcaa catgttttcg
     1321 agatgtatct cattcagcgg cgactcgcgc acgaccagta ccagttttcg ccgctctttg
     1381 agaacgacgt cagccgcgcg atgaatcagg ttgtcgccta gaccgtgcgc gattgccgcg
     1441 acagtgcgca tgctgcatgg tgcaatgacc atgcccagcg tcacaaaaga gccgctcgaa
     1501 attgtggcgc cttgatcgcc gatgccgtgg ctgtgcgtgg ccaggttttg tacgtctttg
     1561 agcgtgtatt gggtttcatg agcgagcgtt cgcgcagccc acttgctgac gacgagatga
     1621 gtctcaacgc cggagccgtg cagcatttgc aagaggcgca cgccaaagat cgtgccggtg
     1681 gcgccggtga tgccgaccac taacctcgct ggctgttgca tacgagaggc attttcacat
     1741 ggatcacaaa aacaaaaaag gccggacgct cgacacgccc gacctgaaca caatctatgt
     1801 aatgtgtggt ttaggccttc actgtgacgt acttctcaac catgcgcgta atcgactctt
     1861 tactcgtggc gccaacgact cgctccactt cctttccctc acggaaaaga atcaaagtcg
     1921 gaattccctt gatgccgtag cgctgagcgg tcgagggatt gtcgtcaaca ttcaacttca
     1981 ccacgcttgc attttcgccg tagtgctcgg cgacggccgc caccgtcggt gccagcattc
     2041 ggcaaggggc acaccattcc gcccaaaaat ccacgagcac gggcttgtct gatcccaata
     2101 catctgtctc gaacgaactg tcattgacgt cttttacgaa ttcactcatc tctggtcgct
     2161 cctaatctgc tgttgatctc gttctgaact cactggccct cgccttcgag gaagttcttc
     2221 aatagccgcg cccgtgatgg gtggcggagt ttgcgcagcg cctttgcctc gatctggcga
     2281 atgcgttcgc gtgttacgtt gaagttctga cctacctctt ccagcgttct ttcatgaccg
     2341 ccgttatcca aaccaaaccg caaacgaata acgcgttctt cacgcgggct cagcgtttgc
     2401 agcacgtcgc cggcaatctc gcgcaaattc gacgtgatga cgcgtgccgc gggactctca
     2461 ctcgatttgt cttcgatcaa gtcgccgagt tgagaatcgc cgttctcgcc gattggtgtt
     2521 tcgagcgaga tcggatcctg catcaacttg cgcgccttgc gaaccttgtc aaccgaggtg
     2581 tccattttct tggcgatctc ctcggtcgtt ggttcgcgtc ccaattcgcg caccagttca
     2641 cgcgaagtgt tcttgagttt attgatcact tcgatcatgt gtaccgggat acgaatcgtg
     2701 cgcgcctggt ccgcgattgc gcgtgtaatt gcctggcgaa tccaccacgt tgcgtacgtg
     2761 gagaacttgt aaccacggcg ccagtcaaac ttgtcgacgc ccttcatcag gcccagattg
     2821 ccttcctgaa taagatccag gaactgcaag ccacggttct gatacttttt cgcaatcgag
     2881 accaccaagc gcaggttcgc ttcggtcagc tcgtgcttcg cctgcgcagt ctgtgcttcg
     2941 ctgacggcga tcatctggtg cgaacgtttg atctccagcg gcgacagatg atgctcggcc
     3001 tcgatctctt tgagacgttt cttagcggcg gcaacctgtt tgcgatactc cttctgcttg
     3061 tcgggtttta atcctttgcg cttcagtttc tcgctcgcgg cctcgatctc acgttccagc
     3121 gcacgcactt ctttatggac gacgccgata gcagcgatga gccgctggcg ggccgcttct
     3181 ttcatatgca gaccgcggat ctcctgagcg atctcgagac gcgtgcgggc gagtttgcgg
     3241 cgatagcgaa gcagcttctt cgagcgcttg ccgcgcgaag ctttctgttc gccagcaaac
     3301 ctttcaaact ccttaagacc ccgcttgtaa agctggcgga tgttgttcaa cccctcgatc
     3361 gtccactgca agtattcttc gctgcggtct tcgtgctcct cggtctccat ctgatccgag
     3421 aacgcgacca cgtcgcggat gttgagtttt ccttcggcga gctcgtcacc gattctgaag
     3481 agctcgacca cagcgattgg cgaacgtgca atcgctttct ctgctcgttt ctggcctcgt
     3541 tcgattcgct tggcgatgga cacttcctgc tcgcgggtca acaaaggcac cacacccatt
     3601 tcgcgcatgt aaacgcgaat gggatcggaa gtcttatcca tctcgccggc cgagagatcc
     3661 aactcgacct cttcaccact catagcttcg tccgactcga cggcagacga agactcttcg
     3721 atcaagggtt cctcgatctc tgtctcactg ctggtggctc tttccagcgt ctgtagtact
     3781 tcgtcgatat cttctatcga cacggcatcg tcggtaagct cttcgctgag attggcgaag
     3841 tcgcccttaa ccgagttcat gagctcgata ccgttttgat ccttttccag cctggacaaa
     3901 ttagctattc ctttctcgct taagcggttt caagaacctt agcgattttc ccattcgact
     3961 ttcctaaggg cttccccagc gacttcccta gtctgggaga tttcacattt gtgagagttc
     4021 cgcacggctt taatcgttgc acgacatcgt cgatcgtgat gtttttcaac acctgctcga
     4081 cggctgcgtc aacctcctgc aacacctctc ccagcaccgt ttcaatattg acgccgacgg
     4141 ggcagtcacg gctcggcggt tggcggtgta atgagaaaac accgccgcac tcaagagcct
     4201 gataaatatc cagaagagtc gtttttgccg gatccgtagc caaccgggaa ccgcccatcg
     4261 agcccgtttg cgatacgacc agaccggctt gcgccaggtc gagcaacata c
//
LOCUS       DASTEY010000636         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_146403, whole genome shotgun sequence.
ACCESSION   DASTEY010000636 DASTEY010000000
VERSION     DASTEY010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146403"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFO72_11105"
     CDS             <1..625
                     /locus_tag="VFO72_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9479886.1"
                     /translation="AVRVIKAHVDYYAAKMGPATDGKTGSMTAVYERAPPYVPLIARS
                     FAARKVPVIIGIYLPVIESGYRHCYEGRFGTNGLYQFLPSTAANYGVSREEMCDVEKM
                     TPAAAHYIADRMAELGEDSQSVTLVLISYNRGAEWVRDSLRQLRGTKNYSRDFWTLLA
                     NQETLDEPFRRETARYVPSFFAAAIIGENPEVFGLQMPALSTLASTR"
     gene            complement(622..1482)
                     /locus_tag="VFO72_11110"
     CDS             complement(622..1482)
                     /locus_tag="VFO72_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479887.1"
                     /translation="MIRHVSGVIAAIAFVILCTVLPFLPGGYDSLAVPLSAMAQIVGT
                     LGLVVVPFGLLWVAADRWQRLAQSRFVFVSLSLIASTLVSAGLSLGAILHSGFALGAL
                     VVVLSVYAVSKAWLKFKQARGTTPLTSTSTWSSAVPFYLITVPVAVALFQFAFVDRAI
                     EFSRNRAILNSSPLIATLEQHRATNGRYPTSLQSVWPDYQPNIIGIREYSYAPHGDSY
                     NLFFFEQFTYRFGTREFVMYNPRDEHVMTAHATDILELTPQQLALERTRGHYARHDLP
                     QPHWKLFWFD"
     gene            complement(1737..>2211)
                     /locus_tag="VFO72_11115"
     CDS             complement(1737..>2211)
                     /locus_tag="VFO72_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479888.1"
                     /translation="QAIVNPAKLSRERQMDLCGLCHGGVGVAKTPPFSFVAGNALADH
                     IQLENPGPNEPLDVHGNQLALLARSTCFKSSKMTCSTCHDVHQPQREVRDFSEKCLQC
                     HKPQSCGLFRTRGRAIVGKCVDCHLPNQNSNVIFSSHDRVRITPKVRNHWIKVYR"
BASE COUNT          512 a          616 c          648 g          435 t
ORIGIN      
        1 ggcggtccgc gttatcaaag ctcatgtgga ctactacgca gccaagatgg gcccggcaac
       61 ggatggtaag actggaagta tgacggcggt ctacgaacga gcaccacctt acgtgccatt
      121 aattgcgcgc tcgtttgcgg cacgcaaagt tccggtgatc atcggaatct atttaccggt
      181 aattgaatcg ggataccgcc attgttacga gggccgtttt ggcacgaatg gtttgtacca
      241 gtttctgccc tcgacggctg caaactacgg cgtttctcgt gaggagatgt gcgacgtgga
      301 aaagatgaca cctgcagccg ctcactacat tgcggaccga atggccgaac tcggcgaaga
      361 ctcgcagagc gtgacgctcg tgttgattag ctataaccgc ggcgcggaat gggtgcgcga
      421 cagtctacgg caactgcgag gcacaaaaaa ctactcaaga gatttctgga cgctgctcgc
      481 caaccaggag acgctggacg aaccctttcg cagggagact gccagatacg taccgagttt
      541 cttcgccgcc gcaatcatcg gcgaaaaccc ggaagtgttt ggcctgcaga tgccggcact
      601 gtcaacactc gcttcaactc gttagtcaaa ccaaaacagt ttccaatgcg gctgcggcaa
      661 atcgtgccgc gcgtaatgcc cacgcgtgcg ctcaagggct aactgctgcg gtgtgagttc
      721 gaggatgtcg gtggcgtggg ccgtcataac gtgttcgtca cgcgggttgt acataacaaa
      781 ctcgcgcgtg ccgaaccgat aggtgaactg ctcgaagaag aacaggttgt agctgtcgcc
      841 gtgcggcgca taactatact ctcgaatgcc aatgatgttg ggctggtagt ccggccacac
      901 ggattggagt gacgttgggt agcggccgtt cgtcgcgcgg tgttgctcga gcgtcgcgat
      961 tagtggcgaa ctgttaagaa ttgcccggtt acgactgaac tcgatcgccc ggtcgacgaa
     1021 cgcaaactgg aacagcgcga ccgcaaccgg cacggtgatc aggtagaagg gcacggcact
     1081 cgaccacgta gaggttgacg tcagcggtgt cgtgcctcgt gcctgcttga acttgagcca
     1141 tgccttagac acggcgtaca ccgacaacac tacgaccagg gcgcctaacg caaaaccact
     1201 gtgcaagatc gctccgaggg acagaccagc cgacaccaac gtggacgcga tcagcgagag
     1261 ggacacgaac acaaatcgcg attgagccaa tcgttgccag cggtcggccg cgacccatag
     1321 cagtccaaac ggcaccacca ccaagcccaa ggtgccgact atctgcgcca tcgcggagag
     1381 tggcacagcc agactgtcat accctccggg aagaaacggc aacaccgtgc aaagaatgac
     1441 gaaggctatc gcagcgatga cgccggatac gtgtctaatc attcgaacag ggtttcgtcg
     1501 cggccgtagt gggtcagttt gcatttaacg ccgaaggctg ctttgcctca aaagtcctct
     1561 ctcattctca ccgggcttta gcccggtgca ggcgcagtga agacggagga aaccgtttca
     1621 acggtttccg ttgattcgcc agcgaaaccg ttaaaacggt ttctcaaaac cgcgtggcgg
     1681 taacaccggg ctgaagcccg gtgagaatga aagaagactt ttgaggcaaa gccgcgctag
     1741 cggtagactt tgatccagtg gttacgcacc ttcggcgtga tgcgaacgcg atcgtggctg
     1801 gagaagatca cgtttgaatt ctggttcggc aggtgacaat caacacactt cccaacgata
     1861 gcgcgcccgc gcgtgcgaaa cagcccgcag ctctgcggct tgtgacactg caaacacttc
     1921 tctgaaaaat cccgcacctc gcgttgcggc tgatggacgt cgtggcacgt cgaacacgtc
     1981 atcttcgaag atttaaagca tgtgctccgt gccagcagcg cgagttgatt gccgtgaacg
     2041 tcgagcggtt cattaggccc ggggttctcg agttgtatgt ggtccgcgag tgcgttaccg
     2101 gcgacaaacg agaacggcgg cgtcttagcc acgccgacgc cgccgtgaca aagtccacaa
     2161 agatccatct gtcgctcgcg tgacagcttc gccgggttca cgatcgcttg t
//
LOCUS       DASTEY010000637         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_525965, whole genome shotgun sequence.
ACCESSION   DASTEY010000637 DASTEY010000000
VERSION     DASTEY010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525965"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFO72_11120"
     CDS             <1..335
                     /locus_tag="VFO72_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9479889.1"
                     /translation="LIATGSEVSLALEARETLQAEGSPTRVVSLPCWELFEEQSNEYR
                     DEVLPPSVTARLAVEAGVGQGWERYVGTSGAVICLDRFGGSAPGEVALRELGFNVENV
                     LKHARALL"
     gene            468..911
                     /gene="rpiB"
                     /locus_tag="VFO72_11125"
     CDS             468..911
                     /gene="rpiB"
                     /locus_tag="VFO72_11125"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET9479890.1"
                     /translation="MRIAVGADHGGYPLNERVIDELRRAGHEVIDFGTHDGSLPDDYP
                     DYAKLVGEAVQTGAAEIGVLVCGSGVGAAVAANKLRGIRAALCGDTYSAHQSREHDDC
                     NVLCLGARVTGVELALDILRSFVAAKFTGEERHRRRLAKVAAMER"
     gene            complement(1001..1969)
                     /locus_tag="VFO72_11130"
     CDS             complement(1001..1969)
                     /locus_tag="VFO72_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HET9479891.1"
                     /translation="MLIIVNNVAAKARQSWPKIKKQLDAAGVNYQTHYTDRQGDATLQ
                     TRVALNSGVQLIVVVGGDGTLSEAAEGFFQFNENLDASPTPINSSATLAVLPAGTGDD
                     FARGLRRSRTPLQEWIDKLIAYVRAERSSSATGPRQIDVMYGLCDGFQKPFICLNAST
                     MGIGGETASRVAAQGGFMRKFSGEFRFVYAAVGALAVWRERRVRVTVDDRVVADGPMN
                     LVGVANGLYAGGGMMFSPEARLDDGKLDVVTASGLSRTNVVTELSRIHTGGHVNNPKV
                     TVTQGASARIETLLLQDAMPLEADGNVKGVTPVQFQVMPRALRFLF"
     gene            complement(1970..3508)
                     /locus_tag="VFO72_11135"
     CDS             complement(1970..3508)
                     /locus_tag="VFO72_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9479892.1"
                     /translation="MIKSDLSEALGVIQSNHVDGKKLDYNSVFKSSITSMLSVLDPHS
                     TYFDPVEYASFRTEQRSEYFGIGATIEDLREGTDVNTYIRATFPEAPAHRAGLRFGDR
                     IVAVDGQSMKGKTYPEVRRFLLGPRGTTVKVTVEHPGTRQEETVSIVRDAVSLPSIAQ
                     AYMLRQGVGYVAMTGGFNLTTDDEFGEALKFLHSKGMNMLVLDLRGNRGGLLIQAVRV
                     DNRFLHRVQPIVTQKGRLKDSSQSYSAVNENPDNVPVVVLVNGETASAAEIVAGALQD
                     HDRALIVGETTFGKGLVQFPFQLDYDSALLLTIAKYFTPSGRLIQRDYSNGGNYSYYF
                     PGGLAAEKKDQATPQTGPETHTDMGRPVYGGGGIAPDEVVKPGTITAAERHLRDVLFA
                     FALELTTGRVAGFESYKIQRPIEFDHDLVNEDYPVTDALFRELKAFAAKKQVFKVTPD
                     QLDKARGYAERQLRYNVLSAAYGYRTATQVFNDADPQIARAVDAMPRARELAVAASRA
                     RARG"
BASE COUNT          767 a         1078 c         1127 g          790 t
ORIGIN      
        1 ttctgatcgc caccggttcc gaagtttcac ttgcgctgga agcacgcgag acactgcaag
       61 ccgagggctc gccaacgcgt gttgtgtcgc tgccatgctg ggagttgttt gaagaacagt
      121 cgaacgaata ccgggacgaa gttttgcctc cgtctgtgac tgcgcgactc gcggttgaag
      181 caggagttgg gcagggatgg gagcggtacg ttggaacgag tggcgcggtg atttgtttgg
      241 accgatttgg cggatcggcg ccaggagaag tcgcgttacg cgagttggga ttcaatgttg
      301 agaacgtgct gaagcacgcg cgcgcgttgt tgtgatcagc tcggacgcgc tcttccctgg
      361 ggcggcaggc gtctcggctg ctcatcggcg cagagcgcgt gaatcgcttc cagcaggcga
      421 gacgcctgcg gtcccaggga agaacaggcg ctttatgtag gaggagtatg cggatcgcag
      481 ttggcgcaga tcatggcggg tatccgctca acgaacgagt gattgatgaa ctgcgccgcg
      541 ccgggcacga agtgatcgac ttcggcactc acgacggatc gctgcctgac gattatccgg
      601 attatgcaaa attagttggg gaggctgtgc agacaggcgc agcggagatc ggtgttttgg
      661 tgtgtggcag cggcgttggc gcggccgtcg ctgcgaacaa acttcgcggt attcgcgcgg
      721 cgctttgtgg cgacacctac tccgcgcatc agtctcgcga acatgacgac tgcaacgtgc
      781 tttgtctcgg cgcgcgtgtg accggagtag aactggcgct ggacattctc cgttcgtttg
      841 tcgcggcgaa gttcacgggc gaagaacgtc atcgccggcg gctggcgaaa gttgccgcaa
      901 tggaacgtta gccacagaca aacacggatt acacacggac tgatttctga tttatgccgt
      961 ccgtgttcgt tcgtgttagt ccgtggcttg cagttctgcg tcaaaataag aaccgcagcg
     1021 cgcgaggcat tacctgaaac tgcaccggtg taacgccctt gacgtttccg tcggcttcca
     1081 acggcatcgc atcctgaagc agaagcgttt caatccgcgc gcttgcgcct tgcgtcactg
     1141 taactttcgg attgttgacg tgaccaccgg tgtggatgcg tgaaagttcc gtgaccacgt
     1201 tcgtgcggct cagccccgaa gcagtgacaa catcgagttt gccgtcatcc aaacgggcct
     1261 cgggcgagaa catcatgccg ccaccggcat aaagaccgtt tgccacgccg acgagattca
     1321 tcggtccatc agcaaccaca cgatcgtcaa ccgtgacgcg cacccgtcgt tcgcgccaca
     1381 cggctaacgc gcccacagcc gcgtacacaa aacgaaactc gcccgagaac ttccgcataa
     1441 aaccaccctg cgcagctact cgactagccg tctcgccgcc aatgcccatc gttgacgcgt
     1501 tcaaacagat gaatggcttt tgaaaaccgt cgcagagacc gtacatcacg tcgatctgcc
     1561 gcggaccggt tgcactggaa ctgcgttcag cacgaacgta agcgatcagc ttgtcgatcc
     1621 attcttgaag cggcgttcga ctgcgccgca atccccgcgc aaagtcgtcg ccggtgccgg
     1681 ccggcaaaac tgccagcgtc gctgaagaat tgattggcgt aggagaagcg tcgagatttt
     1741 cgttgaactg gaaaaaccct tcggcggctt cgcttaacgt gccatcgccg ccgacaacga
     1801 caatgagttg gacaccagaa ttaagggcga cgcgcgtttg cagcgtcgcg tcgccctggc
     1861 gatcggtgta gtgggtttgg taattgacgc ccgcggcgtc gagttgcttc ttaattttcg
     1921 gccatgactg ccgcgcttta gccgcgacgt tgttaacgat gatcagcact tagccgcggg
     1981 cacgcgcacg actggccgcc acggcgagtt cgcgtgcgcg aggcatcgcg tcaaccgcac
     2041 gcgcgatctg cgggtcggcg tcgttgaaga cctgcgttgc agtgcgatag ccgtaggctg
     2101 ccgacagcac gttgtagcgc aactgtcttt cggcataccc gcgcgccttg tcgagttgat
     2161 cgggcgtaac tttgaagacc tgtttcttcg cggcaaatgc cttcagctcg cggaaaagcg
     2221 cgtcagtgac cggataatcc tcgttcacca gatcgtgatc aaactcgatc ggtcgctgaa
     2281 tcttgtacga ctcaaagcca gcaacgcggc cagtcgtgag ttcgagtgca aacgcgaaca
     2341 ggacgtcgcg caggtgacgc tcggccgcgg tgatcgttcc aggcttgacg acttcatccg
     2401 gcgcgatacc gccgccgccg tagaccgggc ggcccatgtc cgtgtgagtc tcgggacccg
     2461 tttgcggagt cgcctgatcc ttcttttcag cggcgagccc acctgggaag tagtagctgt
     2521 aattgccgcc gttcgaatag tcgcgctgaa tcaaacggcc cgaaggcgtg aagtatttgg
     2581 caatcgtcag cagcaaggct gagtcgtagt ccaactgaaa cggaaactgc accagtccct
     2641 tgccgaaagt cgtttcaccg acaatcagcg cacggtcgtg gtcctgaagc gcaccggcca
     2701 caatctcggc cgctgaagca gtttcgccgt tgaccaacac gacaaccgga acgttgtcag
     2761 gattctcgtt cactgccgag tacgactgcg aactatcttt gaggcggcct ttttgcgtaa
     2821 cgatcggctg aacgcgatgc aggaacctgt tatccacgcg caccgcctga atgaggaggc
     2881 cgccacgatt gccacgcaga tcgagcacga gcatgttcat gcccttgctg tgcaggaact
     2941 tcagcgcttc gccgaactca tcgtcggttg tcagattaaa gccgccggtc atcgcgacgt
     3001 aacctacgcc ctgacgaagc atgtacgcct gcgcaattga cggcagcgat acagcatcgc
     3061 ggacgatact gactgtctct tcctggcgag tccccggatg ctcgacagtc actttcaccg
     3121 ttgtaccacg cggtcccaac aggaacctgc gcacctccgg ataggtcttg cctttcatcg
     3181 attggccgtc gactgcgact atgcgatcgc caaaccgcag tccggcgcgg tgcgccggtg
     3241 cctcagggaa ggtggcgcga atgtacgtat ttacgtcagt gccctcacga agatcttcga
     3301 tcgtggcgcc aataccgaaa tattccgaac gctgttcagt gcggaacgac gcgtattcga
     3361 ccgggtcgaa gtaggtcgag tgcgggtcga gcacgctcaa catgctcgtg attgacgatt
     3421 tgaacaccga gttgtaatcg agctttttgc cgtcgacgtg gttcgattga atcacgccga
     3481 gcgcttccga caggtcgctc ttgatcatcg ggctcgtaac aacacctctg ttggtcgcgc
     3541 cggttgtggg cgaagtccga tcgggaggtg tggtccggcg ggccgaacgg ttttgggcga
     3601 gaatcaatac gggaagtgtt aaagcaaaaa taaggaaaag aattcccggg gcaaaagagc
     3661 tttttctcac gcaaccctcc tacttactgt ctaccgctaa tgattacaac cggttcagcg
     3721 cgccgggcct cgccggttcg ggccgggttt ttggcgccgg gt
//
LOCUS       DASTEY010000638         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1432150, whole genome shotgun sequence.
ACCESSION   DASTEY010000638 DASTEY010000000
VERSION     DASTEY010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432150"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2236
                     /locus_tag="VFO72_11140"
     CDS             18..>2236
                     /locus_tag="VFO72_11140"
                     /inference="COORDINATES: protein motif:HMM:NF027230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479893.1"
                     /translation="MPKLLGRIGIRVAPSNPNVVYAIVEAKEGTLYRSDDRGETFRMV
                     SKNDSIVSRGFYYTKVRVDPTNENRIFAVASTLFTSVDGGKTFRSITGRTHIDFHALW
                     LDPKNPKRMWHGQDGGIGVSYDGGESWEAVYNIPLGQFYQIHADNRLPFYYVMGGLQD
                     NGSWTGPSRTREPAGIMNDDWRMVSFGDGFYVINHPDDPEQYLSESQGGDIVWTDFRT
                     REQQAINPWGRGSGGGPAAGHKFRFNWNSPIVFSAHDKTTVFFAGNVVFKSPDFGKTW
                     EQISPDLTTNDPEKLKDAGGPIAIENSTAEYHSTIISIAESPVQKGQIWVGTDDGNLQ
                     LTTDGGKNWRNIIANVSGVAKNSPVSHVEPSRINADTTYISFDRHMFDDFRPYIFKTA
                     DGGKSWTNISGNLPAKAYVWVVREDPKNTNLLYAGTELGLFASYNGGKEWLALNLKNL
                     PNVSIHDLLVHPRENDLILATHGRSIWIFDDATAIQRMNQQILDSNTYLFPVRPAMRF
                     SSRFTRYGIGDKVFTGPNPSAGALITYYLKDKVDEKTTLKIQVFDRDGKLVQDLEKPS
                     REKGLNRIAWNLRVGGPEVRRPPTEEQISFGGAPRGPQVLPGTYTVKLTVGDKMLEER
                     VEVKLDPGIKTSVAELQQTFDMQVKLRDMQSTMNMSLRFLDSLKEQLKQAQTTMKTLN
                     KEPDKDLMKALEDYLKELDAMQDKLAARNDGLGFPGKDQVVDNIGNLFFSLDTNFGPT
                     "
BASE COUNT          547 a          586 c          636 g          467 t
ORIGIN      
        1 acaagttaac gaacggcttg cccaagttgc tggggcgaat cggcatccgc gttgctccaa
       61 gcaatccaaa cgtggtctac gcgatcgtgg aagcgaaaga agggactctc taccgttcgg
      121 acgatcgcgg cgaaacgttc aggatggttt cgaagaacga ctcgattgtt tcgcgcggct
      181 tttactacac aaaggtgcgc gtcgatccga caaacgagaa ccgcatcttt gctgtcgcgt
      241 cgactttgtt tacctcagtt gacggcggca aaacgtttcg ctcgattacg ggccgcacgc
      301 acatcgactt tcacgccctc tggctcgatc cgaagaaccc caaacgaatg tggcacggac
      361 aggacggcgg catcggcgtc agttatgacg gcggtgagtc gtgggaagcc gtttacaaca
      421 ttccgctggg ccagttttat cagatccacg ccgacaaccg gttgcccttc tactacgtga
      481 tgggtggctt gcaggataac ggctcgtgga ctggtccaag ccgcacgcgc gaaccggcgg
      541 gcatcatgaa cgacgattgg cgcatggtaa gtttcggcga cgggttttac gtcatcaatc
      601 atccggacga ccccgagcag tatctttcgg agtcgcaggg cggagacatt gtctggactg
      661 acttccgcac gcgtgaacag caagcgatca atccgtgggg acgaggcagc ggcggcggtc
      721 cggcagcggg acacaagttt cgtttcaact ggaattcgcc gatcgtcttt tctgcgcacg
      781 acaagacgac agtgttcttt gccgggaacg tcgtgttcaa gtcgccggac tttggtaaga
      841 catgggaaca gatcagccca gaccttacta ccaacgatcc tgagaaactg aaagacgcag
      901 gtgggccaat cgcaattgag aattcgaccg cggaatacca ctcgacgatt atcagtatcg
      961 ctgagtctcc cgtgcagaag ggccagatct gggttggcac tgacgatggc aacctgcaac
     1021 tcacaactga tggcggcaaa aactggagaa acatcatcgc gaatgtttcg ggcgtcgcga
     1081 agaactcgcc tgtctcgcac gtggaaccat cgaggataaa cgcggataca acctacatct
     1141 ccttcgaccg gcatatgttc gacgactttc gtccttacat attcaaaacc gcagacggcg
     1201 gaaagtcatg gaccaacatc tccggcaatt taccggcgaa ggcttacgta tgggttgtgc
     1261 gcgaggatcc gaagaacaca aacctgctgt atgcgggaac agagttgggt ctctttgctt
     1321 cttacaacgg cggcaaagag tggctcgcgt tgaacttaaa aaacctgcca aacgtctcga
     1381 ttcacgacct gctcgtgcat ccgcgcgaaa acgatctcat tctcgctacg cacggccgca
     1441 gcatctggat cttcgacgat gccaccgcaa ttcagcggat gaaccagcag attctcgaca
     1501 gcaatacgta tctgtttcca gtgcgaccgg cgatgcggtt ttcgagtcgt ttcactcgtt
     1561 acggtattgg cgacaaagtg ttcactggtc cgaatccctc ggcaggcgcg ttgattacgt
     1621 actatctgaa agacaaggtg gatgagaaga caacgctcaa gattcaagtc ttcgatcgcg
     1681 acggaaagct ggtccaggat ctcgaaaagc cgtcacgcga aaaaggcttg aaccggattg
     1741 cctggaactt gcgagtgggc ggtccggaag tgcgccggcc gccgactgaa gaacagatct
     1801 cgttcggcgg tgcgccgcgt ggccctcagg tgctgccggg aacttatact gtgaaactca
     1861 cggtgggcga caaaatgttg gaagagcgtg tggaggttaa gttagatccg gggatcaaga
     1921 cttcggtcgc cgagcttcag caaacgttcg atatgcaggt caagttacgc gacatgcagt
     1981 cgacgatgaa catgtccttg cgttttctcg acagtttaaa ggagcaactc aagcaggcgc
     2041 agacgacgat gaagacgctc aacaaagagc cggataagga cttgatgaag gcgctcgaag
     2101 attaccttaa agaattggac gcgatgcagg acaagctcgc ggctcgcaac gacggtttgg
     2161 gtttcccggg gaaggatcag gtggtggaca atataggcaa tctgttcttc tcgctcgata
     2221 ccaactttgg accaac
//
LOCUS       DASTEY010000639         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_426664, whole genome shotgun sequence.
ACCESSION   DASTEY010000639 DASTEY010000000
VERSION     DASTEY010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426664"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1083
                     /locus_tag="VFO72_11145"
     CDS             49..1083
                     /locus_tag="VFO72_11145"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HET9479894.1"
                     /translation="MASLLAMRLWIDLANSPHVPFFKALAAKFVECGHEIETTARAFA
                     ETVPLAQAAGFSPHVVGAHGGRQVSAKAGALMSRAWLLATWARKREFDLAISHNSYSQ
                     ILAARALRIRSVTLMDYEYQPANHLAFRLTSRIIVPTSFPARRLRRYGASAGKLRRYH
                     GTKEDVYLADFQPDPSFATELCELGVRPDNVLVLMRPPAHDALYHRFQNKLFDEALAW
                     LLDKENVQVILLPRNDVQRETYSARAGERLIVPANPLDGANLIAASDLVISAGGTINR
                     EAAALGVPVASIYAGPWAAVDQELLKEGRLERISTPEGLRRLPVVKKQSFQPRRAFAV
                     IDEIMRLIFE"
     gene            1092..>2014
                     /locus_tag="VFO72_11150"
     CDS             1092..>2014
                     /locus_tag="VFO72_11150"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479895.1"
                     /translation="MRAERKRQRFTDATAVRVPGRAPKWIIPTVKALLVVTDASAAAG
                     SFILAFYLREGVSAFAGDSLAWSHQFAPYGALMLFVVGIRLLSFRYCDLYRLRGEFSL
                     IDDGIRIFKATAIGSLLIVAAAFLYRGGFEFRAFSYARGVFVVDFFLVLIGVTAIRFV
                     VRSAQTFARQRKVNLIPTLVVGRGPEAALFISEMCERPALGYRVIGVVDTQTPDVHQV
                     GSYEGVPVIATLEGLPEAIRDSGANEVIIADPRVSGDALFEVMMRCGRRRGVEFRIAP
                     SLFNCLPRKTEIDQIGVLPMIRLFREPLSSSA"
BASE COUNT          366 a          527 c          626 g          495 t
ORIGIN      
        1 cgttggcctt ggaaacaact cttcctgttt tgtgctttgt gcttttttgt ggctagtctt
       61 ttagcgatgc gtctctggat cgatctcgca aactcgccgc acgttccgtt cttcaaggca
      121 ctggcggcaa agtttgttga atgcggacat gagatcgaga ccactgcgcg cgcgtttgcg
      181 gaaacggtac cgctggcaca ggcagctggt ttttcacctc atgtcgttgg agcgcacggg
      241 gggcgacaag tgtcggcgaa ggccggcgcc ttgatgagtc gtgcgtggtt gctggcaaca
      301 tgggcccgca aacgcgaatt cgatctggca atcagtcata actcgtattc gcagatcctg
      361 gcggctcgcg cgttgcgaat tcgcagcgtc acgctgatgg actacgaata ccagcccgcg
      421 aatcacctcg cgtttcgctt gacgtcacga atcattgttc ccacgagttt tcctgcgaga
      481 cgattgcgac gttacggcgc gagcgccggc aagttgcgcc gttaccacgg aacgaaggaa
      541 gacgtttacc tcgccgactt tcaaccggat ccgtcgtttg cgacggagtt gtgtgaactc
      601 ggcgtccgcc cggataatgt gctggtcctg atgcggccgc cggcgcacga tgctttgtat
      661 catcgttttc agaacaagct ttttgacgaa gcgttggcgt ggttgttgga caaggaaaac
      721 gtccaggtga ttctgttgcc gcgcaatgac gtccaacgag agacgtattc ggccagggcc
      781 ggcgagagac tgatcgtccc cgcaaacccg ttggacggcg cgaacctgat cgccgcgagc
      841 gatctcgtga tcagtgccgg gggaactatc aaccgagaag ctgcggcgct cggtgttccc
      901 gtggcgagca tttatgccgg gccgtgggcc gcggttgatc aggaactgtt gaaagaagga
      961 cggctggaac ggatctcaac gccggagggc ttgcggaggt tgccggttgt gaagaaacag
     1021 tcgtttcagc cgcgccgtgc gtttgcggtc atcgatgaga tcatgagact gatcttcgaa
     1081 tagcgagctt gatgcgagcc gaacgaaaac gacaacgatt tactgacgcc accgccgtgc
     1141 gcgttccggg tcgtgcgccg aaatggatca ttccgacggt gaaggcgttg ctcgtcgtta
     1201 cagatgcgtc tgcggcggcg ggttcgttca ttctcgcgtt ttaccttcgc gaaggagttt
     1261 cggcttttgc cggcgacagt ctggcgtgga gccatcagtt cgcaccgtac ggcgcgttga
     1321 tgttgtttgt cgtcggcatt cgcttgctga gttttcgtta ctgcgatctc tatcgcttgc
     1381 gtggcgagtt ctcgttaata gacgatggca tccgcatctt caaagcaacg gcgatcggtt
     1441 cgctgctgat tgttgcggca gcgtttcttt atcgcggcgg gtttgagttt cgcgcattct
     1501 cttacgcgcg cggtgtgttc gtcgtcgact tcttcctcgt cctgattggc gtcacggcga
     1561 tcagattcgt cgtgcgttca gcacagacgt ttgcacgaca acgcaaagtc aacttgattc
     1621 cgacgctcgt ggttggacgt ggtccggaag ctgcgctgtt tattagcgaa atgtgtgaac
     1681 gaccggcact cggctaccgc gtgatcggcg tcgttgatac gcagacaccc gacgttcatc
     1741 aggttggaag ttatgaaggc gtgccggtga tcgccacgct tgaagggttg cccgaagcga
     1801 ttcgcgattc cggcgcaaac gaagtgatca ttgccgatcc acgggtgagt ggtgatgcgt
     1861 tgtttgaggt gatgatgcgt tgcgggcggc gtcgtggcgt cgagtttcgc atcgcaccga
     1921 gtttgttcaa ctgtctgcca cgcaagaccg agatcgatca gatcggcgtg ttgccgatga
     1981 ttcgtctctt tcgggaacca ctttcaagca gcgc
//
LOCUS       DASTEY010000640         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1184288, whole genome shotgun sequence.
ACCESSION   DASTEY010000640 DASTEY010000000
VERSION     DASTEY010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184288"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFO72_11155"
     CDS             complement(<1..409)
                     /locus_tag="VFO72_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9479896.1"
                     /translation="MKRSPLVVIFTTVFIDLVGFGIVIPVLPFYAEGTAFNATPRTVG
                     LLFASYSIMQLIFSPVLGGLSDKHGRRPVLLLSIIGTGIGFLILGFAKTLFMLFVGRI
                     LDGITGGNISTAQAYIADITTEENRAKGMGLIGA"
     gene            complement(423..860)
                     /locus_tag="VFO72_11160"
     CDS             complement(423..860)
                     /locus_tag="VFO72_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9479897.1"
                     /translation="MLNRIIATSPTWFTVPIRLALAAVMIAHGSQKVLGTFGGSGFNA
                     FIGGNTPFAFMRPAWLWLALAALSELVGGLLIGLGFLTRVGAFFIACTMLTAVVGVHL
                     SGGFFASNRGYEYPLSLLAMAVALLIAGGGQASVDRALSRGRR"
     gene            970..>2229
                     /locus_tag="VFO72_11165"
     CDS             970..>2229
                     /locus_tag="VFO72_11165"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479898.1"
                     /translation="MHRRRVVGAAALLVVLPFIVAAQTPPVDISFTVAMPRPHTHLLE
                     VDVAIKRTANGPQEEQLVMPVWTPGSYMVREFARHVQDFVATDAANQALKWEKTNKNT
                     WRIVTNGAREWHARYRVYANELSVRTSELNSSHAFWNNANILMYLDGFLKSPSTVRVL
                     APDVWKVATGLPAVLGQKNTFRAENFDVLYDSPFEVSNFKTLIFNVKGVAHRIVIDGE
                     GNYDPERMRRDVQKIVETQVDLMGGEVPYRDYTFILHLRAEGGGGLEHLNSTALGYPR
                     FGFHVERGDRATSASPNTAEALPREYRGFLSLVSHEFFHLWNVKRIRPDALGPFDYTQ
                     ENYTKNLWVAEGITDYYADLVLRRAGLISENEFLSAAARSIQALQNTPGRLEQSVEES
                     SFDSWIKFYRPDENSVNSQISYYDKGAI"
BASE COUNT          505 a          613 c          651 g          460 t
ORIGIN      
        1 cggcgccgat cagtcccatt cctttggcgc ggttttcttc ggtggtgatg tcggcgatgt
       61 acgcttgagc cgtggagatg tttccgccgg taatgccgtc gagaatgcgt ccgacgaaaa
      121 gcatgaaaag ggttttagcg aatccgagta tcagaaagcc gatgccggtg ccgatgatcg
      181 agagtagcag caccggtcgt cgtccatgct tgtctgacaa acctccgagc accggcgaga
      241 agatcagttg catgatcgaa tacgaagcga agagtagtcc caccgtgcgt ggtgtggcgt
      301 tgaaggcggt gccctcggcg tagaacggca ataccggaat gacgatgccg aagccaacca
      361 gatcgatgaa gacggtggta aagatgacga ccagcggtga gcgcttcatt gtgcttgttt
      421 gtttatctgc gtcctctgct caaagcgcgg tcgactgatg cctgcccgcc gccggcaatc
      481 agcagcgcca cggccattgc gagcaggctt aacggatact cgtaaccgcg gtttgacgcg
      541 aagaagccgc cggacaagtg tactcccacc actgccgtca gcatcgtgca ggcgatgaag
      601 aacgcgccca cgcgagttaa gaagccgaga ccgatgagca gaccgccgac cagttcggag
      661 agcgctgcca gggccagcca tagccatgca ggacgcatga aagcgaaagg cgtgttaccc
      721 ccgataaagg cgttgaaacc ggaaccgccg aaagtgccca gtactttctg tgatccgtgc
      781 gcgatcatta cggctgcgag cgccaggcga atcggaacgg taaaccaggt tggagaggtc
      841 gcgataattc gattaagcat gtctaagttg ttcctcgtca ttggacgcag atctacgcgg
      901 attaaaacgc agatctgtag taacattcaa tcgcacttgt catcaacttc tcgaaagatg
      961 ctttactgaa tgcacagacg ccgagtagta ggcgccgccg cgctgctggt agttttgcct
     1021 ttcattgttg cggcgcaaac cccaccggtc gatatttcgt tcaccgtcgc gatgccgcga
     1081 ccgcacacgc acctgctcga agtcgacgtc gccatcaaac gcaccgcaaa cggcccgcag
     1141 gaagagcaac tcgtgatgcc cgtctggacg cctggctcat acatggtgcg cgagttcgcg
     1201 cggcatgtgc aggactttgt cgcaaccgat gctgcgaatc aggcactcaa gtgggaaaag
     1261 acaaacaaaa acacgtggcg catcgtcacc aacggcgcac gcgagtggca cgcacgttat
     1321 cgcgtctacg caaacgagct gtcggtgcgc accagtgagt tgaactcgag ccacgcgttt
     1381 tggaacaacg caaacatcct catgtacctc gacggttttt tgaagagtcc ttcgacggtg
     1441 cgcgtgctcg cgccggacgt ttggaaagtg gcgaccggtc tgccggcggt gctgggccag
     1501 aagaatactt ttcgcgccga gaacttcgac gtcctttacg actcgccttt cgaagtcagc
     1561 aacttcaaga ctctgatctt caacgtgaag ggtgtcgcgc atcggatcgt catcgatggt
     1621 gaaggcaatt acgatccgga acgcatgcga cgcgacgtgc agaagattgt cgaaacgcaa
     1681 gttgatctga tgggcggcga agttccttat cgcgactaca cgtttattct tcacctgcgc
     1741 gctgagggcg gcggcggtct cgaacacctg aactccacgg cacttggtta tccgcgtttt
     1801 ggtttccacg tcgaacgagg cgaccgcgcc acttcagcat cgcccaacac tgccgaagcg
     1861 ctgccacgcg aataccgtgg ttttctctcg ctcgtgtcgc acgagttttt tcacctctgg
     1921 aatgtgaaac ggatcaggcc ggacgcgctc gggccttttg attacacgca ggaaaactac
     1981 accaagaatc tctgggtcgc ggaagggatc actgactact acgcagacct ggtcttgcgt
     2041 cgcgccgggt tgattagcga gaacgagttt ctgagtgccg cggcgcgatc gattcaggcg
     2101 ttgcagaata cgccgggacg tttggagcag agtgtcgagg agtcgagttt cgattcgtgg
     2161 atcaagtttt accgcccgga tgagaactcg gtgaattcgc agatttcgta ctacgacaaa
     2221 ggcgccata
//
LOCUS       DASTEY010000641         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_904869, whole genome shotgun sequence.
ACCESSION   DASTEY010000641 DASTEY010000000
VERSION     DASTEY010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904869"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFO72_11170"
     CDS             complement(<1..639)
                     /locus_tag="VFO72_11170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9479899.1"
                     /translation="MRKIFINDFGRLRSGWRVTIFCFACLAILFVVGNVLRVFYVVLF
                     ELERAPRRDFIPNLVFHITTLIAALGAGYFCARLLEGLPWRSLGLTFHQRWLRDLLIG
                     FAVGFASLALAVVIAAAAGGLRFSLGGAGMIGPILRSLAGSAVMLFVAALAEEALFRG
                     YPLHTFSRAQLASLGVLLTSITFGLAHVSNPNAVPRVTLINTTLAGIWLAVAW"
     gene            725..2968
                     /locus_tag="VFO72_11175"
     CDS             725..2968
                     /locus_tag="VFO72_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9479900.1"
                     /translation="MNDNAENHSPEHRERIRRRRRALLWVLGITVLLLLAIIVSQQLW
                     LWTVVRPDTAADALVLYALSTLNFVAFFVFLFIFVRNLLKLRRERRERQLGSKVKTRL
                     LVYFISISFLPITAMAVFSYLFLNRSLEKWFYVLPEEFLQQATELRRETLSNQHQTLR
                     DTASLLVMLLNQQPLEQRQATMDQIVASGQLVNIEIVANDGSVLLKSRADLPADDAEK
                     LDQLFSEARTYRGPTGANLADGAEFDVIGVPLNQSESLIIAPQKRAATNLFDVVTGSE
                     RDYQDLVRKQRRVRLLGLSTLGLMTLILLFASSWIAIYLARGIATPIKALAEASNEVA
                     RGNLSHRVTTVADDELALLAESFNQMTTQLEENRRRIEVGAAELRDKNLALRERRNYI
                     ETVLQSLTTGVVSLDENDCVTTLNAAASRMLGLAGKLDDNTKLSALIGSEDWIVVDRL
                     LRRARRTGHATEQTQLEGGASGTGVLPVAMTATALRNSVSPRRGVVLVIEDLSELLAA
                     QRAAAWSEVARRMAHEIKNPLTPIQLSAERIAKSYARIAANGNGTNVDGADEKRVAAV
                     ISECTETISREVAGLKAMVDEFSRFARLPATRLGPANLNDVISQAVGLYEERLNGVTI
                     DLSLDPDLPPAMLDVEQIKRVFVNLIDNAIEALSATDEKRITITTTHDTERSLVRAEV
                     ADTGQGIEAGDFRRLFQPYFSKRDSGTGLGLAIVQQIILEHGGRIRAEKNEPHGATFL
                     IELSIAN"
     gene            3153..4499
                     /locus_tag="VFO72_11180"
     CDS             3153..4499
                     /locus_tag="VFO72_11180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9479901.1"
                     /translation="MDSILIVDDERGIRETLRAVMEDEGFVADAVASGEECLKALGKR
                     AYGCILLDVWLPGIDGLETLKQLRAAGSEAAVVIISGHGNIETAVRATKLGAFDFIEK
                     PLSIEKTVLTVRNALRQQQLEFMNANMAAALAREYTMVGESVAMRALRKQISVVAPTD
                     GRVLIYGESGTGKELVARAIHAESRRASAPFVEVNSAAIPEELIESELFGHVKGAFTG
                     ATAAKKGKFELADGATIFLDEVGDMSQKVQAKVLRVLEEQRFEPVGSGTSITVDVRLI
                     AATNKRLDVEIENGNFRADLFYRLNVIPFELPPLRERLEDVPLLVDYFNQHFSRSYGK
                     KPKQFLPEALEELQSYSWPGNVRELRNTIERVVIMHPGTKVSATDLPPHGKEEPPAAS
                     FRFPSFKEATEAYHREFIQRKLEEAEGNVSRAAELMGVDRSHLYRRMRALGIQNRS"
BASE COUNT         1112 a         1317 c         1360 g          986 t
ORIGIN      
        1 ccaggccacg gctagccaga ttcctgcaag cgtggtgttg atcaacgtca cgcggggcac
       61 cgcgtttggg ttcgaaacat gagccaaacc aaaggtgatt gatgtcagca gcactcccaa
      121 cgacgcgagc tgtgcgcgtg aaaaagtgtg tagcggataa cctcggaaca acgcttcttc
      181 cgccaacgcc gctacaaaca acatcacggc ggaaccggct aaagatcgca agatcggccc
      241 gatcattccc gcgccaccta acgaaaagcg caatccaccc gcagccgcag cgataacaac
      301 cgctaacgcc agcgaagcaa aaccgaccgc aaagccaatg agaagatctc gcaaccagcg
      361 ttgatgaaac gtgagtccca gggatcgcca cgggagaccc tcgagcagtc gcgcgcagaa
      421 atatccggcg ccgagtgcgg cgatcagggt tgtaatgtga aagacgagat taggaatgaa
      481 atcgcggcgc ggagcgcgct cgagctcaaa cagcaccaca taaaacacgc gcaagacatt
      541 tccgaccaca aagagaatgg ccaaacaagc gaagcaaaag atggttacac gccatccact
      601 gcgaagccgg ccaaaatcgt tgatgaagat cttgcgcatt gcgcgtcatt ctaagccaca
      661 gactgaacta gagaccaatc tgtgttgtcg gtgcctgtct gtggctgtaa actgactttc
      721 ccgcatgaac gacaacgcgg aaaaccatag cccggaacac agggagcgga ttcgccgtcg
      781 ccgccgtgct ttgctgtggg ttttggggat cacagtcctg ctgttgctcg ccatcatcgt
      841 gtcgcaacag ctctggcttt ggacagtcgt caggcccgat accgccgccg acgcgctcgt
      901 actctacgcc ttatccacac tcaatttcgt agccttcttc gtctttctct ttatttttgt
      961 tcgcaatctt ctcaagctgc gccgtgaaag acgagaacgc cagctcggtt cgaaagtaaa
     1021 gacgcgtctg ctggtctatt tcatctcgat aagctttctg cccatcaccg caatggccgt
     1081 cttctcttat ctatttttga atcgctcgct ggaaaaatgg ttttacgttc tgccggaaga
     1141 gttcctgcaa caggcgaccg aactcagacg cgagacactt tcgaaccagc atcaaacgct
     1201 gcgcgacaca gcctcgctgc tggtgatgtt gttgaaccag caaccactcg aacagcgtca
     1261 ggcgacaatg gaccagatcg tcgcgtcggg tcagcttgtg aatatcgaga tcgttgcgaa
     1321 tgacggcagc gttctgctta agagccgcgc cgatcttccc gcagacgatg cggagaaact
     1381 cgaccaactc ttcagcgagg cgcgcacgta ccgtggtcca accggcgcca accttgccga
     1441 tggcgccgag tttgacgtga tcggcgtgcc gttgaaccag tcagagtcgc tgatcatcgc
     1501 gccacaaaaa cgcgccgcaa caaatctctt cgacgtcgtt accgggtccg aacgcgacta
     1561 ccaggatctc gtgcgcaagc agcgccgcgt gcgtctgctc ggactgtcaa cacttggtct
     1621 gatgacgttg attctgttat tcgcgtcgag ctggatcgcg atctatcttg cgcgcggcat
     1681 tgcaacgcca atcaaagcgc tggctgaggc gtcgaatgag gtcgcgcgcg gcaatctttc
     1741 gcatcgcgtg acgacggttg ctgacgacga gttggcactg ctcgcggagt cgttcaacca
     1801 gatgaccacg cagctcgaag aaaaccggcg tcgcatcgaa gtgggcgcgg cggagttgcg
     1861 cgataagaac ctggccctgc gcgaacggcg caactacatc gagaccgttc ttcagtcgtt
     1921 gactactggt gtggtgtcgc tcgacgagaa cgattgcgtg acaaccttga acgccgccgc
     1981 ctctcggatg ctgggtctcg caggcaagct cgacgacaat acgaaactga gtgcgctgat
     2041 cgggagcgag gactggatcg ttgtcgatcg actgctgcgt cgcgcccgcc gcaccggaca
     2101 tgcaaccgaa cagacgcaac tcgaaggtgg tgcgagtggt actggcgtct tgccggtcgc
     2161 gatgacggct actgctttgc gtaactccgt tagtccgcgc cgcggtgttg tactcgtgat
     2221 cgaagatctc tccgaattac tcgccgcgca gcgagccgct gcctggagcg aagtcgcccg
     2281 ccggatggcc cacgaaatca agaatccatt aacaccgatt caactttccg ccgagaggat
     2341 tgcgaagagt tacgcgcgta ttgccgcgaa cggcaacgga acgaatgtcg atggagccga
     2401 cgaaaaacgc gtggccgcgg tgattagcga gtgcactgaa acgattagcc gcgaagtggc
     2461 ggggctgaaa gcgatggttg acgagttttc gcgatttgcg cgtctgccgg cgacgcggct
     2521 cgggcccgct aatctgaatg acgtgattag tcaggcagtc ggactgtacg aagagcggct
     2581 taacggtgtg actattgact tgagtctgga tccggatctg ccgccggcga tgttggacgt
     2641 ggaacagatc aaacgcgtgt tcgtaaacct gatcgacaat gcaatcgagg ccttgtcggc
     2701 cacggatgaa aaacggatta ccataaccac gacccacgac acagagcgta gtctcgtgcg
     2761 cgcggaagtg gcagacaccg ggcaggggat tgaagcgggg gactttcggc ggttgtttca
     2821 accatacttt tcgaagcgcg acagcggcac tggtttgggc ttggcgatcg ttcagcaaat
     2881 aatactggag cacggcggcc gcatccgcgc cgagaagaac gaaccccacg gcgcgacgtt
     2941 tctaatcgaa ctgagcatcg ccaactgagt gattttcgat ttttgatttt cgattgccga
     3001 ttgatggaat tgccgattgc cgattttcga tttccgattt ggaaaccctg acgctattgc
     3061 gagctgttcg ttcacgttca atcggcaatt ggcaattgcc aatgggcaat tccatcaacc
     3121 ggcaatcgaa aatcaaaaat caaaaatgac ccatggattc tattcttatc gttgacgacg
     3181 aacgcggcat tcgcgaaaca ttgcgcgcag tgatggagga cgaaggcttc gttgccgacg
     3241 ccgtcgccag cggtgaagag tgtctgaaag cgctgggcaa acgcgcttac ggttgcattc
     3301 tgctcgacgt ctggctgccc ggaatcgacg gccttgaaac tttaaaacag ttacgcgcgg
     3361 ccggttctga agccgcggtc gtgatcatct ccggtcatgg caacatcgaa acagccgttc
     3421 gtgcgaccaa gctcggcgcg ttcgacttca tcgaaaaacc gctctcgatc gaaaagaccg
     3481 ttttgactgt gcgcaatgcg ttacgccagc agcagctcga gttcatgaat gcgaacatgg
     3541 cggctgcgct ggcgcgcgag tacaccatgg tcggcgagtc agtcgcaatg cgcgccctgc
     3601 gaaaacaaat ttcggtggtt gcgccgaccg acggccgcgt gctgatttac ggtgaatcag
     3661 gaaccggaaa ggaacttgta gcgcgagcga ttcatgctga gtcgcgtcgc gcttccgcgc
     3721 cgtttgtgga agtcaactct gcggcgatcc cggaggaact gatcgagagt gaactcttcg
     3781 gtcacgtaaa aggcgcgttt acaggcgcga ccgccgcgaa gaaaggaaag tttgaactag
     3841 ccgacggtgc gactatcttt ctggacgaag ttggcgacat gagtcaaaag gtccaggcga
     3901 aggtgctgcg cgtgctggaa gagcagcgtt tcgaaccggt cggcagtggc acgtcgatca
     3961 ccgttgacgt gagactcatc gcggcgacga acaaacgtct cgacgtagag atcgagaacg
     4021 gcaacttccg ggcggatctg ttctatcgtt tgaacgtgat tccgtttgaa ctgccgccgc
     4081 tgcgcgaacg tctcgaagat gtgccgctac tggtggacta cttcaatcaa catttctcac
     4141 gttcctacgg taaaaagccg aaacagtttt tgcccgaggc gctggaggaa ttgcagtcgt
     4201 attcgtggcc tggcaacgtg cgcgaattgc gaaacacgat cgagcgcgtt gtgatcatgc
     4261 accccggcac gaaggtttca gccactgatc tgccgcctca cggcaaggaa gaaccgcccg
     4321 cggcttcgtt tcgtttcccg tcatttaaag aagccacgga ggcgtatcat cgcgagttca
     4381 tccagcgaaa gctggaggaa gctgaaggaa atgtttcccg cgccgcggag ttgatgggtg
     4441 ttgaccgcag tcatctctat cgacgcatgc gcgcgctcgg cattcagaat cggtcttgaa
     4501 ttatccctgc aatctcgcca aagcagttgg caggcctcaa actcccgcgt cggaaacctt
     4561 cgttgacaac gtaagttcaa atagcttact agttaacttc tcgtgcgtca cgtagtgggg
     4621 cgagaaagaa gttgagggga aatggatttt acgacgaaag aactgttgaa gcggtacgca
     4681 agactcgcga tcggacaacc cttttcgccg gtattgctaa acatactagt cacgtccgtc
     4741 tgcgacatgc gctgcacgca ctgctttttc actga
//
LOCUS       DASTEY010000642         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1399082, whole genome shotgun sequence.
ACCESSION   DASTEY010000642 DASTEY010000000
VERSION     DASTEY010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399082"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFO72_11185"
     CDS             complement(<1..283)
                     /locus_tag="VFO72_11185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9479902.1"
                     /translation="MQPDTLGHWGKYGGRFVPETLMAPLEELTDAYLSSRDDESFQTE
                     LQDLLSNYAGRPTPLFWAARLTEQSGGAKIYLKREDLLHSGAHKINNTLG"
     gene            complement(292..1257)
                     /locus_tag="VFO72_11190"
     CDS             complement(292..1257)
                     /locus_tag="VFO72_11190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9479903.1"
                     /translation="MASTGLTMSARAEKRNTARTTERARGRVLLIADDDQTRFVEAIT
                     ARGINVVGVANGAAAMVSLQRSRPHVVVANPSARGLGIRELAKMLARSDDGIPLVLAG
                     SQPATLDVRHAALLEGAFDYFELPLELELLIERTQQLVAVRQKIDQLRSDADLDSLTG
                     LANRRRFRVALSREVERWRRYRVPCALLMLDIDHLKRINDQFGHPAGDAVIRQIAQTL
                     KEVSRDNDTAARLGGEEFALLLAGVDMQKAAAAAERLRTVLSGRPVEGVGTVTVSIGV
                     AACPENAPSERTLYVAGDRALYVSKNSGRDRVSVAPPLQEKLPGV"
     gene            complement(1347..1967)
                     /locus_tag="VFO72_11195"
     CDS             complement(1347..1967)
                     /locus_tag="VFO72_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9479904.1"
                     /translation="MTLVKICGITSLEDALAAVDAGADALGFNFYRPSPRYISPVAAR
                     AITDQLPESIVKAGVFVNEELESVVRIVNEAGLSAVQLHGDESPDYCRELRGRYVIKA
                     FGAVNDLDFADYQVDAIMLDTKDELLRGGTGRVFDWIIAQRARQSVKSVALFLAGGLS
                     PDNVAEAIATVRPYAVDACSSLEDAPGRKNHERMRAFVKAARSVTP"
     gene            complement(1964..>2310)
                     /locus_tag="VFO72_11200"
     CDS             complement(1964..>2310)
                     /locus_tag="VFO72_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479905.1"
                     /translation="NSQLESLRRLTEDELGMDALVEVHNSQEMDRAAGCGATLIGVNN
                     RNLRTFEVSLDTSLKLAPLAPRDALLVSESGLNEAADLRRLRDQGFHGFLIGESLMRA
                     SDPGAALRDLRT"
BASE COUNT          506 a          716 c          604 g          484 t
ORIGIN      
        1 gacctagcgt gttgttgatc ttgtgcgcgc cactgtgcag caggtcttcg cgtttgagat
       61 agatcttcgc gccgccactc tgttcggtaa gacgcgccgc ccaaaacaga ggcgtcggcc
      121 gcccggcgta attactgagc aggtcttgaa gttctgtttg gaaggattcg tcgtcacgag
      181 acgagaggta cgcatcggtc aactcttcga gcggcgccat cagcgtctcg ggaacaaagc
      241 gcccgccgta cttgccccaa tgacccaatg tatcaggttg catttcttga attacacacc
      301 gggcaatttt tcctgtaacg gcggcgccac cgaaacgcga tctcttccgg agttcttaga
      361 gacgtaaagc gctcggtcgc cggccacata caacgtccgc tcggatggcg cattctcagg
      421 acaagccgcg actccgatac taacagtcac ggttccaacg ccttcaacag gccggccgct
      481 gagaactgtg cgcaaacgtt ccgctgcagc cgcagccttt tgcatgtcga caccggccaa
      541 caaaagtgca aactcttccc cacccagacg cgcggccgtg tcgttgtcac gcgaaacttc
      601 cttcaacgtc tgcgcaatct gccgaatcac agcatcgccg gccggatgtc caaactgatc
      661 gttgatccgt tttagatgat cgatatcgag catcagtaac gcacacggca cacgataacg
      721 ccgccacctt tctacttccc gcgacaacgc cacgcgaaaa cggcgacggt ttgccaagcc
      781 ggtcaacgaa tccaagtcgg catccgatct cagttgatcg attttttgtc gcacggcgac
      841 taattgctgc gttctctcga tcagcaattc caactcgagc ggcaattcga aatagtcgaa
      901 agcgccttcg agcagcgccg cgtgacgaac gtcgagagtt gccggctgcg aaccggccag
      961 caccagcgga ataccgtcat ccgatcgcgc cagcatcttc gcgagttccc ggattcccaa
     1021 gccacgcgcc gacgggtttg caaccaccac gtgcggacgc gatcgttgta aggaaaccat
     1081 cgccgccgcg ccgttcgcga cgccgacgac gttgattccc cgcgccgtga ttgcttccac
     1141 aaaccgcgtt tgatcgtcgt cggcgatcag caacacgcgc ccgcgcgcac gctcggttgt
     1201 cctggcagta tttcgttttt cagcccgcgc actcatagtc agacctgtgg aggccaacaa
     1261 atcaaacaaa cctgtttcgg gaggacgttc agaaagtttt aggttttggg gcaagggtta
     1321 gaatactccg cgaacgtagg gcagcgtcaa ggtgtcacac tccgggcggc tttaacaaag
     1381 gcgcgcatcc tctcgtgatt ctttctcccg ggcgcgtctt ccaaagaact gcaagcgtct
     1441 acagcgtaag gtcggacggt ggcgatcgct tccgcgacgt tgtcgggcga aagtccaccg
     1501 gccagaaaaa gggcgacgga tttcacggat tgacgcgctc gttgcgcaat gatccagtca
     1561 aatacgcgac ccgtgccgcc gcgcagaagt tcgtccttcg tgtcgagcat gatcgcgtcg
     1621 acctgatagt ccgcaaaatc cagatcattt acagcgccga acgctttgat aacgtatctc
     1681 ccccgcagtt cgcggcagta gtctggcgac tcatcaccgt gaagttgtac ggcggacagt
     1741 cctgcttcat tgacaatgcg gaccacggat tcgagctctt cgtttacgaa cacgcccgct
     1801 ttaacaatcg attccgggag ttgatcggtt atcgcgcgcg cagcgactgg cgaaatgtaa
     1861 cgcgggctgg gccggtaaaa attaaaaccg agtgcatccg cgcccgcgtc cacggcagcg
     1921 agcgcatctt cgagactggt tatcccgcaa atcttaacca gggtcatgtt cttaaatccc
     1981 gaagtgcggc gccgggatca ctcgcacgca tcaacgattc gccaatcaaa aatccgtgaa
     2041 atccctggtc tcgcagccgt cgcaaatcag ccgcctcatt caaaccgctt tcgctcacca
     2101 acaacgcatc gcgtggggcc agcggagcta gttttagtga cgtatcgagc gacacttcaa
     2161 acgttctaag attccgattg ttcactccga tgagcgtcgc tccgcagcca gcggcccgat
     2221 ccatttcctg tgaattatgc acctcaacga gcgcatccat ccccagctcg tcttccgtca
     2281 agcggcgcaa cgattcgagc tggctattgt
//
LOCUS       DASTEY010000643         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_179593, whole genome shotgun sequence.
ACCESSION   DASTEY010000643 DASTEY010000000
VERSION     DASTEY010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179593"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2124
                     /locus_tag="VFO72_11205"
     CDS             <1..>2124
                     /locus_tag="VFO72_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9479906.1"
                     /translation="ALLGVLKAGGVCVPLDPGYPSERLRLLIEDSHASVVLTQERLVQ
                     AISGSGAHVVCLDVFDSLIGSSTEVPTRADPASVAYVIYTSGSSGRPKGVMVTHGALC
                     NHMRWMRDAFAFDEADVFLQKTPIGFDASVWEFYIPLMVRAKLVMARPGGHQDGGYLV
                     RTIIEQGVTVVQLVPMLLRMLIDELGFSSCEDLRLVFCGGEALAKELAAQCLERLPAA
                     RLCNLYGPAETTIDATYWEAKDRTNAATMPIGMPVANVGAYVLDQSLRPVPIGVVGEL
                     YLDGAGLARGYEGRADLTADRFVPNPFRNEPGTRMYRTGDLARRLTGGELEFLGRIDG
                     QVKVRGSRVELGEVESVLSAHADVTAAALIGREDTSGERRLVAYVVTAAERAGLAREL
                     RSYLKERLPGHMVPSVFVRLEQLPLLPSGKVDRRALPAPDLFELTPQGEYRPPRTVVE
                     ETLCGIWEEVLGRDLIGADDDFFELGGHSLLATQVVSRVKEALSVELPLSRIFQTPVL
                     SEIAEFLERRLRGGGKHTEPPLQPVSRERPLPLSFAQQRLWFLDQLEPANPLYNTPGA
                     LRLLGRLDVTALERALSEIVRRHEVLRTTFQTVEGIPVQSISAPHPVTIALIDLSDLH
                     EVDRECEAQRLISAEAERPFDLSHGPLLRATLLRLGEDEHLLLFTMHHIISDGWSVGV
                     LAREATRLYATFTGSEAAPLTERPVQ"
BASE COUNT          425 a          553 c          691 g          455 t
ORIGIN      
        1 gctttgctgg gggtgctgaa agcgggtggc gtctgcgtgc cgctcgatcc tggctatcca
       61 tccgagcggc tgagattact gattgaagat tcacacgcga gtgtggtgct gacgcaagag
      121 aggttggtcc aagccatatc cggaagcggt gcgcacgtgg tctgtctcga cgtgttcgat
      181 agtctcattg gtagctcaac agaagttcct actcgagcgg atcctgcgag tgtagcttac
      241 gtgatctaca cgtcgggatc gtcgggccgg ccaaaaggcg tgatggtgac tcacggcgcg
      301 ctctgcaacc acatgcgttg gatgcgcgac gcgtttgcgt tcgacgaagc agacgtgttt
      361 ctgcaaaaga cacccatcgg tttcgacgcc tcagtgtggg agttctacat acccctgatg
      421 gtgagggcga agttagtaat ggcgcgtccc ggtggacacc aggacggcgg gtatctggta
      481 cgcacgatca tcgagcaggg cgtgacggtg gtccagttag tgccgatgct gttgcggatg
      541 ctgatcgacg aattgggctt tagttcgtgt gaagacctga ggctggtgtt ctgcggcggc
      601 gaggctttag caaaagaact cgcggcgcaa tgtcttgaac gattgcctgc cgcgcgtttg
      661 tgcaatctat atggaccagc agaaacaacg atagacgcca cttactggga ggcaaaagat
      721 cggaccaacg ctgcaacgat gccaatcgga atgccggttg ccaatgtagg ggcgtacgtt
      781 ttggaccaaa gcctaagacc tgtgccgata ggagtagtgg gagagttgta cctggacggc
      841 gcgggattgg cgcgcggtta cgaagggcgc gcggatctga cggcagatcg attcgtgccg
      901 aacccgttcc gaaacgagcc cggcacacgc atgtaccgca cgggcgacct cgcgcgtcga
      961 ctaacaggag gagaactcga gtttctgggt cgtatcgacg gacaggtaaa ggtgcgtggt
     1021 tcgcgcgtcg agttgggtga ggtcgagtcg gtgctgtctg ctcacgcgga cgtgaccgcg
     1081 gcagcgttga tcggacgcga ggatacgtcg ggcgagcgcc ggttggtggc gtacgtggtg
     1141 actgcggctg aacgggccgg cctggcacgc gagcttcgca gttatttaaa ggagcgcctg
     1201 ccgggccaca tggttccgag tgtgttcgtg cgcctcgagc agttgccgtt gttgccgagc
     1261 ggaaaagtgg atcgtcgcgc gctaccggcg ccggatctgt ttgagttgac cccgcagggc
     1321 gagtatcgac cgccacgcac agtggtggag gaaacgttgt gcggaatttg ggaagaagtg
     1381 cttggtcgag atctgatagg cgcggacgac gacttcttcg aacttggcgg ccattcactg
     1441 ctcgctacgc aagtcgtatc gcgtgtgaaa gaagctttat cggtggagtt gccgctcagc
     1501 cggatctttc aaacaccggt actttcggaa atagcggagt tcttagagcg ccgtctgcga
     1561 ggtgggggca aacacactga acctccacta caacccgtat cgcgcgagcg gccattacca
     1621 ctatcgttcg ctcaacaaag actttggttt ctcgatcagt tagaaccggc gaatccttta
     1681 tataacactc ccggcgctct gcgtttgctg ggccggctcg acgttaccgc actcgagcga
     1741 gctttgagcg aaatcgtgcg ccggcatgaa gtgctgcgta cgaccttcca gaccgtagag
     1801 ggaattccgg tgcagagcat ttcagcgccg cacccggtga ccattgcatt gatcgattta
     1861 tcagacctgc acgaagtcga tcgcgaatgt gaggcgcaac gattaattag cgctgaagcg
     1921 gagcgtccct tcgacctgtc tcatgggcca ctgctccgtg cgacgttgtt gcggctgggt
     1981 gaagatgagc acctgctgtt gttcacgatg caccacatta tcagcgacgg ttggtccgtc
     2041 ggtgtcctcg cacgtgaagc gacgagactt tacgcgactt tcaccgggtc agaggcggcg
     2101 ccgctcacag agcgtcccgt gcaa
//
LOCUS       DASTEY010000644         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_393567, whole genome shotgun sequence.
ACCESSION   DASTEY010000644 DASTEY010000000
VERSION     DASTEY010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393567"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFO72_11210"
     CDS             <1..248
                     /locus_tag="VFO72_11210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9479907.1"
                     /translation="IISSRVGGQPEAIRDNVNGFLCEPDRLEQFVERVEQLAQDDDLR
                     RRLSTEARKIAIEEFDNEIYTTRLIAYYNRVRRTANR"
     gene            268..1680
                     /gene="mpl"
                     /locus_tag="VFO72_11215"
     CDS             268..1680
                     /gene="mpl"
                     /locus_tag="VFO72_11215"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HET9479908.1"
                     /translation="MHYHLIGICGTAMASLAGMLQARGHRVTGSDQYVYPPMSTMLRD
                     LGIDLLQGFRAEHLTPAPDCVIIGNAIPRGNPEVEETLNRKLLYRSQAEVVKEEFIRG
                     RHSLAVAGTHGKTTTTSIAAWVMDQGGLDPTFLIGGVAQNFGASFRVTESDYFVIEAD
                     EYDTAYFDKGPKFMHYLPELAIVNNIEFDHADIYKDLDAVKLAFRRFMNLVPSNGRLI
                     AGWDSPNVRAVVESFGSRLFTNVETFGTTSDAKWQVRNADFSGRLSKFEVLREGQSWG
                     EFETPLLGEFNLLNCLAIIIAADAWGVSRERIKDALASFKNVKRRAEVRGEQKGVVVI
                     DDFAHHPTAVRETLRALRSRYGGRRLIAIFEPRSWSSRLAVFQDDYANAFAAADYVVI
                     ASVFDSQKVTEKGRALDTNELIKAISQQGKPAFALPDADHIVAHIVPELRAGDVVAIM
                     SNGGFGGIHDKILSALASES"
     gene            1892..2449
                     /locus_tag="VFO72_11220"
     CDS             1892..2449
                     /locus_tag="VFO72_11220"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HET9479909.1"
                     /translation="MKNFSIFEQALKFSGVEQTENLGEMASLARRDFADRESFIFHTT
                     TASVEYVCLLIENAVLLRTNRAGRVYAGVEKLSSLQPIIDRYMRIADVSEAVYLFGQD
                     DWRPPRHPNIRLVTLKDDFRLAREWFVIAQSPSLNCALVAFDETGPESLPEQIRYWAL
                     KTSNPLAVRGLANAVDGVIDWTLAA"
     gene            complement(2387..2854)
                     /locus_tag="VFO72_11225"
     CDS             complement(2387..2854)
                     /locus_tag="VFO72_11225"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET9479910.1"
                     /translation="MSEVKLRSRMKNLLMFVPNLVLLCVRLMIDPRVPATERVLVAGA
                     ILYALIPLDFIPDMLPFVGQVDDAYLIAISLLRLMTVTDPRVVRQHWRGGGDVVELVG
                     STALIAKKLLPARIRRVLTAQVEVRRPLAGAIESGSERPIDHAVDSIRQPTNR"
BASE COUNT          657 a          802 c          809 g          644 t
ORIGIN      
        1 cgattatcag ttcacgagtc ggtggtcagc cggaagcgat ccgcgacaac gtgaacggtt
       61 ttctctgcga accggatcgc ctcgagcagt tcgtcgagcg agttgagcaa ctggcacagg
      121 acgatgacct caggagacga ctctcgaccg aggcccgaaa gattgcgatc gaagaattcg
      181 ataacgagat ttacactacg cgcttgatcg cctattacaa ccgggtccgg agaactgcta
      241 atcgataaat caattcgttc atacgatatg cactatcacc tgattggaat ctgcggtacc
      301 gccatggctt ccctcgcggg aatgcttcag gcgcgcgggc atcgggtgac tggatcggat
      361 caatacgtct atccgccaat gtcaacgatg cttcgcgatc tcggtattga cctgttgcag
      421 ggatttcgtg cggaacacct gaccccggcg ccggattgcg tgatcatcgg gaacgcgatt
      481 ccacgaggta atccggaagt cgaagagacg ctcaatcgca aactgcttta cagatcgcag
      541 gcagaagtcg ttaaagaaga attcatccgc ggccgtcact cgctggctgt cgcaggcacg
      601 cacggaaaaa caacgacgac cagcattgcc gcgtgggtca tggatcaagg tggactcgat
      661 ccaactttcc tgatcggcgg tgtggcgcag aacttcggcg ccagttttcg cgtcaccgaa
      721 agcgactact tcgtcatcga ggctgacgaa tacgacacgg cttatttcga taaaggtccg
      781 aagttcatgc actacctgcc cgaactcgcc atcgtcaaca acatcgagtt tgatcacgcc
      841 gacatctaca aagatctcga cgcggtgaag ttggcgttca ggcgtttcat gaatctcgtg
      901 ccgtcgaatg gaaggttgat tgcgggctgg gacagtccaa acgtacgtgc cgtagtcgaa
      961 tctttcggtt ccagactctt tacgaacgtg gaaacttttg gcactacaag cgatgcgaag
     1021 tggcaggttc gcaatgctga tttcagtggt cgactttcga agtttgaagt tttgcgcgaa
     1081 ggtcagagtt ggggtgaatt cgagacgccg ctactgggag agttcaatct actgaattgt
     1141 ctcgcaataa tcatcgccgc ggatgcgtgg ggcgtgagcc gcgaacgaat caaggatgca
     1201 ctcgcaagtt tcaaaaacgt gaaacgccgt gccgaagtgc gtggcgagca gaaaggagtt
     1261 gtggtgatcg acgacttcgc gcatcaccct accgcagtca gagaaacgct gcgcgcctta
     1321 cgctcgcgat atggcgggcg tcgcctgatc gcgatttttg agccgcgttc ctggtcctct
     1381 cgcctcgcag tgtttcagga cgactatgcc aatgcgttcg cggcggccga ctatgtagtc
     1441 attgccagcg tcttcgatag tcagaaggtg acagaaaagg gacgagcgct tgatacgaac
     1501 gaattaatta aagcgatttc gcaacaaggc aagcccgcgt ttgcgctgcc cgatgcggac
     1561 cacatcgtcg cgcacatcgt tccggagctg cgggccggcg acgtggtcgc cattatgtcc
     1621 aacggcggtt tcggcggcat tcacgataag atcctcagcg ccctggcgtc ggaaagttaa
     1681 ttcatgccgt taaacctggg acgcaggcgt ctcgcctgca cacccaccac agcgtccgat
     1741 tcgacagttg gcaggcgaga cgcctgcggt cccaggtaag cgttcaccgc taaataattt
     1801 cacgcacaca ctttaacgaa ctattcgtaa cgctctcgca gacttcctcc ggtaaaaccc
     1861 actcccggtt gaccgccagg aggccccctg cgtgaaaaat ttttccatct tcgagcaggc
     1921 actcaagttc tcaggcgtcg aacagacgga gaacctcggt gagatggcga gtcttgctcg
     1981 ccgcgacttt gcggaccgcg aaagttttat cttccacacg actactgctt cggttgagta
     2041 cgtgtgcctg ttgattgaga acgcggttct gcttcgcacc aatcgtgccg gacgcgttta
     2101 tgccggtgtc gagaaacttt cttcgctgca acccatcatc gatcgctaca tgcgtatcgc
     2161 cgacgtctcc gaggccgtgt atctttttgg ccaggacgat tggcgaccgc cgcgacatcc
     2221 aaatatccgg ctcgtcactt tgaaggatga ttttcgcctg gcgcgtgagt ggtttgtgat
     2281 cgctcaatca ccgtcgttga attgtgcgct ggtggcgttt gatgaaacag gtccggagag
     2341 tttgcccgaa cagatccggt actgggcact gaagacgagc aacccgttag cggttcgtgg
     2401 gctggcgaat gctgtcgacg gcgtgatcga ttggacgctc gctgcctgat tcaatcgcgc
     2461 ctgctaacgg tcgacgaacc tcaacctgtg cggtcaacac gcggcgaatt ctcgctggca
     2521 ataacttctt cgcaattagc gcagtcgatc cgaccagttc caccacgtcg ccgccgccgc
     2581 gccagtgctg tcgcaccacg cgtggatccg ttaccgtcat cagtcgcaga agtgagatcg
     2641 cgatgaggta ggcatcgtcg acctggccca cgaacggcag catgtcagga atgaaatcga
     2701 gtgggattaa ggcgtagaga attgctcccg caactaaaac tctttctgtt gccggtacgc
     2761 gcgggtcgat catcaaccgc acgcagagca gcaccaggtt agggacaaac atcaagagat
     2821 ttttcatgcg gcttcgcagc tttacttctg acatgccagc cattatacag attacgcaat
     2881 ggacattctc gaacgaattc gccaacgcgc cg
//
LOCUS       DASTEY010000645         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_14669, whole genome shotgun sequence.
ACCESSION   DASTEY010000645 DASTEY010000000
VERSION     DASTEY010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14669"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFO72_11230"
     CDS             complement(<1..523)
                     /locus_tag="VFO72_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET9479911.1"
                     /translation="MPNDKYTPLPEHKFSFGLWTVGNRGRDPFGDAVRPTLTPVEAVR
                     MLGEIGAYGVNFHDNDLVPIDATPQERDRIVNEFRRACEENGIVVPMATVNLFYDPVF
                     RDGAFTANDKSVRAYALQKTMRAMDLGAECGAKIFVLWGGREGVETDACRRPDDAVKR
                     LREELNYLCEYSLD"
     gene            complement(538..1431)
                     /locus_tag="VFO72_11235"
     CDS             complement(538..1431)
                     /locus_tag="VFO72_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET9479912.1"
                     /translation="MGSGSQNLRLAFVTNNSSDFWTIARKGTEKADAELADVTVDFRI
                     PSEGTAADQKRLMDDLMSAGVQGIAMSPVDPENQTQAINEAAKRVLLVTQDSDAPQSD
                     RAFYVGTDNVAAGRLAGELIKEALPQGGKIMLFVGKLDARNAQERLLGIKETLAGSNI
                     QVIDVRTDDTDRVRAKANAADTLVNHSDVGALVGLWSYNGPAILGAVRDANKIGQVKI
                     IAFDEEDETLSGVKDGSIYATVVQQPYEFGYQAIRMMRDYLKGEKSAIPASKQVFIPA
                     LVVKQDTVEEFTKKINQLRGR"
     gene            complement(1515..>2507)
                     /gene="galE"
                     /locus_tag="VFO72_11240"
     CDS             complement(1515..>2507)
                     /gene="galE"
                     /locus_tag="VFO72_11240"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET9479913.1"
                     /translation="GGAGYIGSVTTQLLRARGQQVVVLDNLSRGHRGAVAPDIPFYEG
                     NVGDRELVASIARTHQIDACIHFAAFAYVGESVSNPALYYENNVEQGVRLLGALVESG
                     IRQLVFSSTCATYGEPQRIPIDEEHPQLPTNPYGWSKLFLERMMADYDCAYGLKFVAL
                     RYFNASGATSDRGEHHDPETHLIPLVLKTAQGEFEHVAVFGNDYPTKDGTCVRDYIHV
                     LDLAEAHLLSLHYLRAGNASTAINLGNGQGYSVLEVIGTAREVTGAEIKARVQERRPG
                     DPSHLVADSSRAHSVLGWQPQHAELREIVGSAWQWHTEHVRGYDQNSTSKRRTA"
BASE COUNT          532 a          713 c          678 g          584 t
ORIGIN      
        1 tgtcgagtga atactcacac agataattca gctcctctct taatctcttt accgcatcat
       61 caggacggcg gcaggcgtcg gtttcgacac cttcacgccc gccccacaac acgaagatct
      121 ttgcgccaca ctcagcaccc aaatccattg cccgcatggt cttttgcagc gcgtatgcgc
      181 gcaccgattt gtcgttggcc gtaaacgcac cgtcacggaa aaccggatcg taaaagagat
      241 tcactgtggc catcggtacc acgattccgt tttcttcaca ggcacgacga aactcgttta
      301 cgattcgatc acgctcctga ggcgtggcgt caatcggaac aaggtcgtta tcgtgaaagt
      361 tgacaccgta ggcgccgatc tcgcccagca ttcgcaccgc ctcaaccggt gtgagcgtgg
      421 gacgaaccgc atcgccaaat ggatcgcgac cacgattacc aactgtccaa agcccaaaac
      481 tgaatttatg ttcggggagc ggtgtgtact tgtcgtttgg catttctccc tccttaatca
      541 ccgtccgcgg agttgattga tctttttcgt gaactcctcg acagtgtcct gcttaaccac
      601 gagagcaggt atgaatactt gtttgcttgc aggaatcgcc gacttctcac ctttcagata
      661 atcgcgcatc atcctgatcg cctggtaacc aaactcgtaa ggttgttgca caacggtggc
      721 gtagatggag ccgtctttga cacctgagag agtctcgtct tcttcatcga aagcaatgat
      781 cttaacctgt ccgatcttgt ttgcgtctct cacagcaccg agaatagcgg gaccgttgta
      841 gctccatagt ccgacgagtg cgccgacgtc cgaatgattg acgagagtat ccgcagcgtt
      901 tgccttcgca cgcacgcgat ccgtgtcgtc ggttctgaca tcgatgacct gaatattcga
      961 gcctgccaat gtttccttaa ttcccaggag acgctcttgc gcattgcgtg cgtcaagctt
     1021 gcccacgaag agcatgatct tgccgccttg cggcagcgct tctttgatca gctcgccggc
     1081 gagtcgtcct gcggcgacgt tgtcggtgcc gacgtagaac gcgcggtcgc tctgcggggc
     1141 gtcggagtcc tgcgtcacca gcagcacgcg ttttgctgct tcgttaatcg cctgcgtctg
     1201 gttctccgga tcgacaggac tcatggcgat accctgaaca cccgctgaca tcaggtcgtc
     1261 catcagtcgt ttctgatcag cggccgtgcc ttcagatgga attctaaaat ccactgtcac
     1321 gtccgcgagt tcggcgtcag ccttttcagt gccctttcga gcgatggtcc aaaaatccga
     1381 actgttgttg gtgacgaagg ccaggcggag gttctgtgaa ccggatccca cagttccgtc
     1441 gggcgccggg gcaggttgac atgacgcaag taagaccagg catgcacaga tgaacacgct
     1501 ccgagtgacg attttcatgc tgtcctccgt ttagatgtcg aattttggtc gtagcctcga
     1561 acatgctctg tgtgccactg ccacgctgac ccgacaatct ctctaagctc agcatgctgc
     1621 ggctgccaac caagaactga atgcgcgcgc gaagagtccg cgacaagatg cgatgggtca
     1681 cccggacgac gttcttgcac ccgggctttg atttctgcgc cggtaacttc gcgagctgtt
     1741 ccgatcactt caaggactga gtagccttgt ccgttgccga gattaattgc ggtggacgca
     1801 ttgccggcgc gcaggtagtg caacgagagt aagtgcgcct cagcgagatc aagcacgtga
     1861 atgtagtcac gcacacacgt gccgtctttg gtcgggtagt cgttaccgaa aacggcaacg
     1921 tgctcgaatt cgccttgcgc agttttcagc acgagtggaa tcaggtgcgt ttcgggatcg
     1981 tgatgttcac cgcgatctga ggtggcgccg gaagcgttga agtagcgaag ggcaacaaac
     2041 ttcagtccgt atgcacaatc gtagtcggcc atcatgcgtt cgagaaagag tttggaccat
     2101 ccgtagggat tcgttggcag ctgcggatgc tcttcatcga ttggaatgcg ttgcggttcg
     2161 ccataggttg cgcacgttga agagaagacc agttggcgaa ttcctgattc aacgagagcg
     2221 cccagcagtc gaacaccctg ttctacgttg ttctcgtaat agagagccgg attgctgacc
     2281 gattcgccca cgtaggcgaa ggctgcaaag tgaatgcacg catcgatttg gtgcgtgcgc
     2341 gcgatactgg cgaccagctc ccggtcgccc acattacctt cgtaaaaagg aatgtcgggc
     2401 gcgaccgcgc cgcgatggcc gcgcgacaag ttatccaaaa caaccacttg ctggccgcgc
     2461 gcacgcagca actgagtagt aacactgcct atatatccgg cgccgcc
//
LOCUS       DASTEY010000646         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1613338, whole genome shotgun sequence.
ACCESSION   DASTEY010000646 DASTEY010000000
VERSION     DASTEY010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613338"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFO72_11245"
     CDS             <1..224
                     /locus_tag="VFO72_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479914.1"
                     /translation="AGFEVHVLVQQAEPDATRTHDVATIRCLVTSDETKDRTLAGAVS
                     TYKSDVFSRTDLQRRASQHVLNAIGFMYF"
     gene            complement(262..1479)
                     /locus_tag="VFO72_11250"
     CDS             complement(262..1479)
                     /locus_tag="VFO72_11250"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET9479915.1"
                     /translation="MRKIGKVFAVTCLIVAGLIVVGCSSGGSQTNDNMVAATVNGRNI
                     MLQEVERAVNRQAGGNPSALSQLQLAQARLTVLSNLIQREVLFQRAEKEKVLPTEAQI
                     DGAIAQQKEQTGMTQEDFEKSLKAQNMSIDALREEARKDLAITALQDKYSGKITISDR
                     EVEDFYNSNRQQFKNERGVALAMIAVDPADNSATGIADDAKNEADAKLKIDNIHQQLQ
                     GKADFAIVARARSEDINSLRGGGDIGFATEEDLKANGFPPELISSFFGPMQVGDYTPP
                     INFRGKWYIFKLADKRLQTENLTLESPGVRQQITQGLTNQRKQILNAALLEVAINEAR
                     IINNLAANMLNNPGNLGLRPAGEATPAQQQQPTQAPAASPAATAPATNSPGASSSPVS
                     VRPGNANSANANR"
     gene            1580..1774
                     /gene="rpmB"
                     /locus_tag="VFO72_11255"
     CDS             1580..1774
                     /gene="rpmB"
                     /locus_tag="VFO72_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET9479916.1"
                     /translation="MAKRCEVCGKGPQFGNNVSHANNRTPRRFDPNLQSIRVQMPQGG
                     ARRMKVCTTCIKAGKVAKAA"
     gene            complement(1836..2219)
                     /locus_tag="VFO72_11260"
     CDS             complement(1836..2219)
                     /locus_tag="VFO72_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9479917.1"
                     /translation="MKERVLTDKAPAAIGPYSQAIRAGGFVFVSGQIPIDPATGEFVT
                     GGIGEQTERVLQNLSAVLEAAGSGLDKVVKTTVFLADMSEFAAMNEVYAKFFTAAPPA
                     RATVAAAGLPRDARVEIEAVALLSE"
BASE COUNT          472 a          698 c          612 g          556 t
ORIGIN      
        1 gagccgggtt tgaagtgcac gtgctggtcc aacaggccga gccggacgcc acgcgcacgc
       61 acgacgtcgc cacgatccgc tgtctcgtca cctctgacga gacgaaagac cgtactcttg
      121 ccggcgccgt ttcgacctac aagtccgacg tgttctcccg gactgacttg caaagacgcg
      181 cctcgcaaca cgtcttgaac gccataggat ttatgtactt ctgaaagacg aaacagcatc
      241 cactgactgc acaggatcga tctatctatt tgcgttcgcg ctattcgcgt tgccaggccg
      301 caccgatacg ggcgaactgc tcgctcccgg actattcgtt gccggagctg tagctgccgg
      361 cgaggccgct ggggcttgtg tcggctgttg ctgctgtgcc ggagtggctt cgcctgccgg
      421 acgcagaccc aggttgccgg ggttgttcag catgtttgcg gcgagattgt tgataatgcg
      481 cgcctcgtta atcgccacct cgagcaacgc ggcattcaaa atctgcttgc gctgattggt
      541 gagaccctgc gtgatctgct ggcgcacacc cggactctcg agtgtgagat tctcagtctg
      601 caaccgctta tcggcgagct tgaagatgta ccacttgcct ctaaagttaa tcggtggcgt
      661 gtaatcgcca acctgcatcg gaccgaagaa actcgaaatc agctccgggg ggaaaccgtt
      721 ggcttttaga tcttcttccg ttgcaaaacc gatgtcgccg ccgccgcgca gcgagttgat
      781 atcttcgctc cgcgcacgag cgacgattgc gaaatcggct ttgccctgca actgctgatg
      841 gatgttgtcg atcttcaact tcgcgtcggc ctcgttcttc gcatcgtcgg cgatccctgt
      901 tgcactgtta tcggccgggt ccaccgcgat cattgccagc gccacgccgc gctcgttctt
      961 aaactgttgc cggttgctgt tgtaaaagtc ttcaacttcg cgatcgctga tggtgatttt
     1021 gccgctgtac ttatcctgaa gtgcagtgat cgccagatcc ttgcgcgctt cttcgcgcag
     1081 cgcgtcgata gacatgttct gcgctttcaa actcttctcg aagtcttcct gcgtcatgcc
     1141 ggtctgctct ttttgctgtg cgatcgcgcc atcgatctgg gcttcagtcg gaagcacctt
     1201 ctctttctct gcgcgttgaa agagcacctc acgctggatc agattgctga gcacggtgag
     1261 ccgcgcctgg gccaactgta gttgcgacaa cgcggaagga ttgccgcctg cctgcctgtt
     1321 cacggcccgc tctacttcct gcagcatgat gttgcgtccg ttgacggttg cggccaccat
     1381 gttgtcgttg gtttgtgaac cgccggacga acacccaacc acaatgagac cagcaacgat
     1441 cagacacgtg acagcgaaga cttttccaat tttgcgcact ttaactctgc tcctttacgt
     1501 tcacttacca gccgcttgct tgacgacccg gaaacccgtt tgttataaag cttgattttg
     1561 taattaggag gttgaacaaa tggctaaacg ttgcgaagtc tgcggcaagg gacctcaatt
     1621 cggcaacaat gtctcgcacg cgaataatcg cacgccgcga cgcttcgatc cgaatctaca
     1681 atcgattcgc gtacagatgc cgcagggtgg cgctcgccgg atgaaggtct gcacgacctg
     1741 cattaaagcg ggcaaagtcg ccaaggctgc ttaacgcttc catcccaatt tacttgtcaa
     1801 cctccaaccg gcgcgcgctc aagccgccgg ttgtttcatt ctgaaagcaa cgccacagct
     1861 tcgatctcca ctcgagcatc gcgcggcaaa ccggctgctg ctaccgtcgc cctggccggc
     1921 ggcgctgccg tgaagaactt cgcgtaaacc tcgttcattg cagcaaactc gctcatgtct
     1981 gccaaaaaga ctgtggtctt cacgacctta tcaagcccgc tacctgccgc ctcgagtacc
     2041 gccgaaaggt tctgcaacac acgttcggtt tgttcgccga ttccgccggt aacaaattct
     2101 cccgtagccg gatcaatcgg aatctggccg gagacaaaaa caaaccctcc tgcacgaatg
     2161 gcctgcgaat aaggaccaat cgccgccggt gctttgtctg tcagaacgcg ttctttcacg
     2221 gtaaacaaag gcgggattct agcacgaata gacaggattt cagaattgac aaagactatt
     2281 ttggtagtgt cctaagtagt gaaacacgct ctttcctggg accgcaggcg tctcgcct
//
LOCUS       DASTEY010000647         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1596870, whole genome shotgun sequence.
ACCESSION   DASTEY010000647 DASTEY010000000
VERSION     DASTEY010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596870"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..617)
                     /locus_tag="VFO72_11265"
     CDS             complement(36..617)
                     /locus_tag="VFO72_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479918.1"
                     /translation="MTRRVLLLGLFFTLALTSSCSGSLFRVKPATELPPLPEHSRSAE
                     AGGVTVRVAPLLTDEESQELFDANLPLSGVLPLRVELVFQSGVPVEVKKAKFRLRDSQ
                     NREWKLLSPKSAISRIMKANGVRLYNPNSRKQFEQEFGAYAIDLKPPLSSSDPRRQGF
                     LFFQTPDKKAVTADQQLTLTVERLPQTATITVN"
     gene            689..1255
                     /locus_tag="VFO72_11270"
     CDS             689..1255
                     /locus_tag="VFO72_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9479919.1"
                     /translation="MIRPFRGVHPQIHPTAYVDPSAQVIGDVLLGEEASIWCNCTVRG
                     DIHYIRIGDRSNLQDNCVIHVQNGTHPTILEEEVTVGHSVTLHGCHVERGSLIGIGSI
                     LLDDARIGEFSLVAAGSLVSPKTIIPPRSLAMGIPARVKRPLTDQEVADLAQFWQNYV
                     NYTREYLQEQFVQAVHKDTQIVRGISEE"
     gene            1666..3042
                     /gene="hisS"
                     /locus_tag="VFO72_11275"
     CDS             1666..3042
                     /gene="hisS"
                     /locus_tag="VFO72_11275"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET9479920.1"
                     /translation="MPAKTQPARGMRDFLPEDVRKREYVIGVVKEVYERYGFEPLETP
                     AAENIETLMGKYGEEGNQLIFKILKRGVHEGTGEADLALRYDLTVPLARVVAEYRDKL
                     PRFFKRYQIQPVWRADRPARGRFREFYQCDVDVLGSRSVIVETEICAAACEVLRKLGF
                     NDFCMRLNHRKALTGILAVAGVALEKHEAALIALDKLDKIGREGVEKEFAARGINGVA
                     GFSLLNFFSALTELEHAADLADQTPQALNRAILGRIVEFVGDNELGAQGVDELQSIID
                     FADAAGIGNRLKIDPSLARGLSYYTGAIMEMHVKDLAGSLGGGGRYDNLVGMFLGQDV
                     PACGFSLGLERILVVMSEKNMFPPHVGTAPADVMVAIWDEVSLAESIALAQTLRAEGL
                     RVDLYPEADKLGKQFKYAASRGVAFVAVLGDEERAQGKVGLKDMKSGEQRTVTRNEVV
                     QLVRGSES"
     gene            3046..3558
                     /locus_tag="VFO72_11280"
     CDS             3046..3558
                     /locus_tag="VFO72_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HET9479921.1"
                     /translation="MSHEAVAQNYLDDARKAMRAYKKLAEKALDQLNDEEFFVTLDEE
                     SNSVAVIMKHMAGNMFSRWTDFLTTDGEKPNRNRDYEFVIEPGTTKNDVRNYWERGWE
                     CVFAALDPLQTEDLNRRVLIRGEEHTVVEAINRQLMHYAYHVGQIVFLAKHFRAIAWQ
                     SLSIPRNRAI"
     gene            3560..>4374
                     /gene="aspS"
                     /locus_tag="VFO72_11285"
     CDS             3560..>4374
                     /gene="aspS"
                     /locus_tag="VFO72_11285"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET9479922.1"
                     /translation="MLDHLGNLERTHSCGELRATDIGRQVTLMGWVARRRDFGELTFI
                     DLRDRGGITQVVLNPEVSATAHAKAKELRGEYVIAVTGNVVMRDENTRNPKIVTGDVE
                     VHGSELLILNDARTPPFQLDAPPDALASEDLRLKYRYLDLRRPQLQSHLLLRHRVLNE
                     IRRYMTAAGFTEIETPILIKSTPEGARDYIVPSRIFPGKFFALPQSPQLFKQLCMIGG
                     LDKYFQIAHCFRDEDLRADRQPEFTQLDVEMSFATLDQIYTLIEGLFVSVFKLV"
BASE COUNT         1009 a         1119 c         1276 g          970 t
ORIGIN      
        1 gattccgcag agtcttgtgt aatctgtgaa tggttctagt tgacagtaat tgtagcggtc
       61 tgcggtaacc gttcgacagt cagcgttagc tgctgatcgg cggttaccgc ttttttgtcc
      121 ggtgtttgga agaacagaaa accctgccgt cgcgggtcgc tggaggataa tggcggtttt
      181 agatcgatgg cgtaagcgcc aaactcttgt tcgaattgtt tgcgtgagtt tggattatag
      241 agtctgacgc cgttcgcctt cataatgcga gagatcgcgc ttttgggaga cagcagtttc
      301 cactcgcgat tctgactgtc acgcaaacga aacttcgctt ttttcacttc gacgggcacg
      361 ccgctttgaa agactaactc cacgcgcaac ggcaacactc cgctgagcgg caaatttgcg
      421 tcaaagagtt cctggctttc ttcatccgtc agcaagggcg cgacacgaac agttacgcca
      481 ccagcttcgg cgctacgact gtgttccggc agaggcggca actcggtcgc aggcttcact
      541 ctgaaaagcg aaccgctgca cgaactagtc agtgcgagag tgaaaaagag gcccagcaac
      601 aacacgcgtc gggtcatgcg gagcaattta gcacaggcat gaacacggaa gagaactgat
      661 tagcgactgt ggtactatcc gacaaaccat gattagaccg tttcgtgggg ttcacccgca
      721 gattcaccca accgcgtacg tggacccaag cgcgcaagta atcggcgacg tactgctcgg
      781 cgaagaagcc tccatctggt gcaactgcac cgttcgtggc gatattcatt acattcgcat
      841 cggtgatcgc tcgaaccttc aggacaattg cgtgattcac gtgcagaacg gaacgcaccc
      901 gaccatcctc gaggaggagg tcactgtggg ccacagtgtt acgttgcacg gttgtcacgt
      961 tgaacgaggt tcgttgattg gcatcggctc gattctgctg gatgacgctc gcatcggtga
     1021 gttctcgctg gttgccgccg gatcgcttgt gagtccgaag acgataattc caccgcgatc
     1081 tttagcgatg ggaattccgg cgcgagtgaa gcgcccgctg accgatcagg aagtggccga
     1141 cctcgcgcag ttttggcaga actacgtcaa ttacacacgg gagtatttac aagaacaatt
     1201 cgtacaggct gtgcacaaag atactcagat cgtgcggggg atctctgagg aatgacatgt
     1261 ttctcacgca aagacgccaa gacgcaaaga aaacccttag aaatacgatc cgctttgcgc
     1321 ctttgcgcct ttgcgcgaga ggttcttctg gtcagagagg ctttgcccga aaagtgattt
     1381 ttacttgatg cttctgctga gaagtgctcc ggaggagcaa gatgtttata gccttacgtt
     1441 tttattaact agtgcgctcc agcggagcgc aatgtgctct ggccgttcgg tttacatacc
     1501 gctcctgacg gagcgaagta attcaggaac cgaagagcta taaatatttt gctcctccgg
     1561 agcaagacgc ttaggtgcaa ataaaatggc ttttcggaca aagctggtca gagatacttt
     1621 ttgtgcaaag ccattcgtac aggaacaacc acccctaagt agataatgcc agcgaagact
     1681 caaccggcgc gcggcatgcg cgacttcttg cccgaagacg ttcgcaaacg cgaatacgtc
     1741 atcggtgtgg tcaaggaggt ctacgagcgc tacggtttcg agccgctgga gacgccggca
     1801 gccgagaaca tcgaaacgtt gatgggcaag tatggcgaag aaggaaacca gcttatcttc
     1861 aagatactga aacgcggcgt gcatgaagga acgggcgaag cagatcttgc gctgcgttac
     1921 gatctgacgg tgccgctggc gcgtgtggtt gcagagtatc gcgacaagct gccgaggttt
     1981 ttcaagcgtt atcaaattca gccggtgtgg agggcggatc gtcctgcgcg tggaaggttc
     2041 agagaatttt atcagtgtga tgtcgatgtg ctggggtcgc gatcggtgat cgtcgagaca
     2101 gagatttgtg cggcggcgtg tgaggttttg cggaagcttg gtttcaatga tttctgcatg
     2161 cggctcaatc accgcaaggc gttgaccggc attcttgcgg ttgcgggtgt tgcgttggag
     2221 aagcacgagg ctgcgctgat cgcgctcgac aagctcgata agattggacg cgagggagtt
     2281 gagaaggaat tcgccgcgcg cggtatcaat ggcgtcgcag gttttagtct tctgaatttt
     2341 ttctcggctc tgacggagtt ggagcacgct gcggaccttg cggatcaaac accacaagca
     2401 ctgaacaggg cgatcctcgg acgcattgtg gaatttgtcg gcgacaacga actcggggcg
     2461 caaggcgttg acgagttgca gtcgatcatt gattttgccg acgcggctgg aattggcaac
     2521 cggctcaaaa tcgatccgag tctcgcgcgt ggactttcgt attacaccgg cgcgatcatg
     2581 gaaatgcacg tgaaggatct cgctgggagt ctgggtggtg gtggccgtta cgataatctc
     2641 gtgggcatgt tcctcggaca ggatgtgccg gcgtgtggct tttcgctcgg cctggagaga
     2701 atcctggtgg tcatgagcga gaagaacatg tttccgccgc acgtgggcac ggcgccggcc
     2761 gacgtgatgg tagcgatctg ggatgaagtt tcgctggccg agtcgattgc gttagcgcag
     2821 acgttgcgcg cggaaggttt gcgagttgat ctgtatccgg aggctgataa gctcggtaaa
     2881 cagttcaagt acgctgcttc acgcggcgtt gcatttgttg cggtgctcgg cgacgaggag
     2941 cgcgcgcagg ggaaagtcgg tctcaaagac atgaaatcag gcgagcagcg aactgttacc
     3001 cgaaacgaag tcgtgcagct cgtgcgagga agcgagagtt agatcgtgtc acacgaagca
     3061 gttgcccaaa actatctcga cgacgcgcgg aaggcgatgc gcgcgtacaa gaaacttgcc
     3121 gaaaaggcac tcgaccaact gaacgacgag gagttcttcg tcacgctgga cgaggaatca
     3181 aacagcgtag ccgtgatcat gaaacacatg gccggcaaca tgttttcgcg ctggactgac
     3241 ttcctcacaa ctgacggcga gaaaccgaat cgcaaccgcg attacgaatt cgtaatcgaa
     3301 cccggcacaa cgaaaaacga cgtgcgcaat tattgggaac gtggttggga gtgcgtcttc
     3361 gctgctctcg acccgttgca aactgaagat ttgaatagga gagtgcttat tcgcggcgag
     3421 gaacacacag tcgtcgaggc aatcaatcgc caactgatgc actacgcgta tcacgtcggc
     3481 caaatcgtgt ttctggctaa acactttcgc gccatcgcct ggcagtcatt aagtattcca
     3541 cgaaacagag ccatttagaa tgttggatca tctcggaaac ctggaacgca cgcattcatg
     3601 tggtgaactc cgtgccacgg acatcggcag gcaagtaacg ctcatgggct gggtcgcgag
     3661 gcgtcgtgac tttggcgaac ttacgtttat cgatctgcgc gaccgcggtg gtatcacaca
     3721 ggtcgtcctg aacccggaag tctcagctac ggctcacgcg aaagcgaagg aattgcgcgg
     3781 cgaatacgtg atcgcggtca ccggcaacgt cgtgatgcgc gacgaaaaca cgcgcaaccc
     3841 gaagatcgtc accggcgacg tagaggttca cggcagcgag ctgctgattc tcaacgacgc
     3901 gcgcacgcct ccgtttcaac tcgacgcgcc gcctgatgcg ctcgccagcg aggatctgcg
     3961 cttgaagtat cgctacctcg acctgcgccg gccgcagctt cagtcgcact tgctcctgcg
     4021 tcatcgtgtg ctcaacgaaa tccggcgcta catgaccgcc gcagggttca ccgaaatcga
     4081 aacgccgatt ctgatcaagt caacgcccga aggcgcgcgc gactacatcg tgccgtcacg
     4141 aatcttcccg ggaaaatttt ttgctctgcc gcagtcgcca caactcttca agcagttgtg
     4201 catgatcggc ggtctcgata agtactttca aatagcgcac tgttttcgtg acgaagacct
     4261 gcgcgccgat cgacaacctg agttcacaca gctcgacgtc gagatgagtt ttgcaacact
     4321 cgatcagatc tacaccttga tcgaagggct gttcgtctcc gtgttcaaac tcgt
//
LOCUS       DASTEY010000648         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_739851, whole genome shotgun sequence.
ACCESSION   DASTEY010000648 DASTEY010000000
VERSION     DASTEY010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739851"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..2049)
                     /gene="asnB"
                     /locus_tag="VFO72_11290"
     CDS             complement(121..2049)
                     /gene="asnB"
                     /locus_tag="VFO72_11290"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9479923.1"
                     /translation="MCGIAGIVSRQSDVLIGPMLESIEHRGRDDQGVWVSEPIDDSGR
                     RACLGHRRLAIIDPTPAGHEPMLSPDGRYVLTFNGEIYNYRELREQLRPRGHQFKTDC
                     DAEVLLAAFAEWGHEGITRLNGMFAFAVWDNRERTLTLVRDHVGIKPLYYASTPDAFI
                     FGSEIKAILASKLIAPQIDPEALHQFLTFLWAPDPNTLFAGVKTLPPGHYLQFKDGDV
                     KLTQWWDVSFSTIEEHHSDKWWQERVLETLDRVVKMEMVADVPLGSFLSGGLDSSGIV
                     AMMDRHSNGHKISTYTAGITSEDLRYDIIPDDVRWARRVNEQFQTDYHEIMLQPSVAE
                     LLPKLVHHMEEPPIDMAIPSYLISRAARETLTVMLSGMGGDEVFAGYPRQMAMKIAAA
                     LDPVPDLLRRPLMKTIAGVLPGGLPGRLTAPLRNAKKFARSAVLDFTDRYLGYGTYFT
                     DQMKSQLYSGDWRALTRGYDAYATHRRYFARVHDVAPLNKLLYVDLKTFLPCLNLMTT
                     DKTSMAANLEVRVPFLNREMIEMAARLPPRLKLRGLTRKYVLKRALESVLPKEVVWRK
                     KAGFGAPIRSWLRGPLRPLVDDLLSEEMVRRRGMFRPAEVKRVIDANFSGREDYNLHV
                     FQLLGLELWLREFRVNFL"
     gene            complement(2025..4142)
                     /locus_tag="VFO72_11295"
     CDS             complement(2025..4142)
                     /locus_tag="VFO72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HET9479924.1"
                     /translation="MKQVLQNRKTRRPFVGEVPVPALQRGRVLVRTVASLISAGTERA
                     AVELVSKGLVQEARERPDLVKAVVAKVKSEGLLNTFAAVRDKMGASQALGYSASGIVT
                     AVAEDVSEFHVGDRVACGGVGFASHAEVLSVPKNLCVHLPEAVSFEGGAYGTLGAIAL
                     QGVRLAEPTLGESVVVIGLGLVGQLTVQLLKANGCRVFGIDIDPSRVSLALELGADAS
                     LVSGDETEKKINEWTRGRGADAVLITAATDSNQPVELAAKVSRLKGRVIVVGMTGLDI
                     PRQPFFSRELKLIISMSYGPGRYDPDYEEKGHDYPLPYVRWTEKRNIESFLELVADKR
                     VNVDRLTTHRFRIEDAECAYELISGDSAEPYIGVLLNYDPEAPVARRVALGARPPARK
                     SERSIVLGVIGAGGYVPAMLLPHFKSEGVELRSIATASGITAHDVGMRFGFAAAGGAN
                     DVLDDADINLVVIGTRHDLHAQLAREALERNKNVFVEKPLALNDDQLDAVLEAAGRST
                     GRLMVGFNRRFSPPAQEAKEFFRNRETPLSIIYRVNAGHIPKEHWPQDPVEGGGRIIG
                     EVCHFIDLMQFFTDARPVSVFAESVSAKSPRIIDADSVFITLRFADGSNGVIAYLSEG
                     DKALAKERIEIFGGGKTFVVDDFRSGKTQDKGQRAQVQRLCACLREGAPAPISLAELA
                     ATTRATFRVLDSLRSKCVVSLES"
BASE COUNT          942 a         1375 c         1116 g          887 t
ORIGIN      
        1 tttcaacggt tttccgttga ttcgccagcg aaaccgttaa aacggtttct caaaaccgcg
       61 tggcggtaac accgggctga agccccggtg agaatgagag aagacttttg aggcaatgcg
      121 tcagagaaag ttaacccgga attctctaag ccataactcc agccccaaca actggaaaac
      181 gtgcagattg tagtcctcgc ggccggaaaa gttcgcgtca atgacccttt tcacctcagc
      241 gggccgaaac atgccccggc gtcgtaccat ctcctccgaa agcagatcgt caaccagcgg
      301 ccgcagcggt ccccgcaacc aggagcgaat cggcgcgcca aagcccgcct tcttccgcca
      361 aacgacctct ttcggcagga cgctttcgag cgctcgcttc agcacgtact ttctggtcaa
      421 tcctcgcagc ttcagccgcg gcggcagacg agccgccatc tcgatcatct cgcggttgag
      481 aaacggcacc cgaacctcga ggtttgcggc catcgacgtc ttatcggtcg tcatgaggtt
      541 gagacaaggc aggaaagttt tcagatcaac atagagcagc ttgttcagcg gcgcgacgtc
      601 gtgaactcgg gcgaagtaac gccgatgagt ggcatacgca tcatacccgc gcgtcaatgc
      661 gcgccagtcg cccgaataga gctgcgactt catttgatcg gtgaagtatg tgccataccc
      721 gagatatcga tcggtgaagt cgagcacggc actgcgcgca aacttctttg cattgcgtaa
      781 cggcgcagtc agtcgacccg gcaagcctcc cggcaacacg ccggcgattg ttttcatcaa
      841 cggccgccgc agtaaatcag gcaccggatc gagagcggca gctatcttca tcgccatctg
      901 gcgcgggtaa cctgcgaaca cctcgtcgcc tcccataccc gacaacatca ccgtcaacgt
      961 ttctcttgcg gcccgcgaga tgaggtagct gggaatcgcc atgtcgatcg gcggctcttc
     1021 catgtgatgg accagcttcg gcagcagctc agcgaccgaa ggctgcagca taatctcgtg
     1081 atagtcagtt tggaactgct cgttcacgcg gcgcgcccaa cgcacgtcgt cgggaatgat
     1141 gtcgtaacgc agatcttcgc tcgtgatgcc ggcggtgtag gtactgatct tatggccgtt
     1201 gctgtggcgg tccatcatcg ccacgatgcc tgacgagtcg agcccgcccg atagaaacga
     1261 gcccagcgga acatcagcca ccatctccat cttcacgacg cgatcgagtg tttcgagtac
     1321 gcgttcctgc caccacttgt cgctgtgatg ctcctcgatc gtgctgaacg agacgtccca
     1381 ccactgggtg agtttgacgt cgccgtcttt aaactggagg tagtggccgg gcggcagcgt
     1441 tttgacgccg gcgaacaacg tgtttggatc gggcgcccaa agaaaagtga gaaactggtg
     1501 cagcgcctcg gggtcgattt gcggcgcaat cagtttcgaa gcaaggatcg ctttgatctc
     1561 ggaaccaaag ataaacgcgt caggggtgct ggcgtaatac aacggtttga taccgacgtg
     1621 atcccgaacc agcgtcaaag ttcgctcacg gttgtcccaa actgcgaaag caaacatccc
     1681 attcagacgg gtgattcctt cgtggcccca ctcagcgaaa gcagccagca ggacttcggc
     1741 gtcgcagtct gttttgaact ggtgtccgcg cgggcgaagc tgttcgcgca gttcgcggta
     1801 gttataaatt tccccgttga aggtgagcac gtaacggccg tcgggagaaa gcatcggctc
     1861 gtggccggcg ggagtgggat cgatgatggc gagacggcga tggccgagac aggcgcgccg
     1921 gccggaatca tcgatcggct ctgaaaccca aacgccctga tcgtcgcgac cgcgatgctc
     1981 gatactttcg agcatcggtc caatcagcac gtcggattgg cggcttacga ttccagcgat
     2041 accacacatt tggatcgaag agagtcgagc acacgaaagg tggcccgggt ggtagctgcc
     2101 agttcagcga gactgatcgg ggcgggtgcg ccttcgcgca agcaggcgca cagccgttgt
     2161 acctgtgccc gctgaccttt gtcttgtgtt ttcccgcttc ggaaatcgtc aactacaaaa
     2221 gtctttccgc cgccgaagat ctcgatgcgt tctttagcca acgctttgtc gccttcactc
     2281 agataggcga tgaccccatt cgaaccgtcg gcaaaccgca aagtgatgaa gacagagtca
     2341 gcgtcaataa ttctcggcga cttcgcactc actgactcag caaatacaga caccggtctc
     2401 gcgtccgtaa aaaattgcat cagatcgatg aagtgacaaa cctcgccgat aatgcgcccg
     2461 ccgccttcaa ccggatcctg cggccaatgt tccttcggaa tgtgacccgc gttcacgcga
     2521 tagattatcg acaacggtgt ttcgcgattg cggaagaact cctttgcctc ctgcgccggc
     2581 ggcgagaagc gcctgttgaa gccaaccata agacggcccg tcgatctccc ggcggcttct
     2641 aaaaccgcgt ccaactgatc gtcgttgagc gccagcggtt tttcgacaaa cacgttcttg
     2701 tttcgctcga gcgcttcccg tgcaagctgg gcgtgcagat cgtgtcgcgt accaatcaca
     2761 acaagattta tgtccgcgtc atcgaggacg tcattcgcac cacccgctgc cgcgaaccca
     2821 aatctcatcc caacatcgtg cgccgtgata cccgacgccg tcgcaatcga gcgaagctca
     2881 acgccttcac tcttgaagtg cggcaacaac atcgcgggaa cgtaaccgcc cgcgccgatc
     2941 acgccgagca cgatcgatct ttccgacttg cgcgccggcg gtcttgctcc cagcgcgacg
     3001 cgtcgcgcaa cgggcgcttc cggatcgtaa ttcaacaaca ctccaatata tggctccgcc
     3061 gaatcacccg agattaactc gtacgcgcat tctgcgtctt caatgcggaa ccggtgtgtt
     3121 gtcagccgat cgacgttcac gcgtttgtca gccaccagct cgagaaagct ctcgatgttc
     3181 cgcttttcgg tccagcggac gtaaggtagc ggatagtcgt gtcccttctc ctcgtaatcc
     3241 ggatcgtacc ggcctggtcc atacgacatc gaaatgatca gtttgagttc gcgactgaag
     3301 aaaggctgtc gcggaatatc gagtccggtc atgcccacta cgatcacacg gcctttcaaa
     3361 cgtgacactt tggctgccag ctcaacgggc tggttcgagt ccgtggccgc agttatcagc
     3421 acggcgtccg caccgcgtcc ccgggtccat tcattaatct ttttttccgt ttcatccccg
     3481 gaaacaaggg acgcatcagc acccagctcc agtgccagtg aaacccggct cggatcgata
     3541 tctataccaa acacccgaca gccgttcgct ttcaacaact gcaccgtcag ttgtccaacc
     3601 aaacccaatc cgatcacaac caccgactca cccagcgtcg gctccgcaag ccggacacct
     3661 tgcaaagcga tcgcgccgag cgttccgtaa gctccgcctt caaaactcac cgcctccgga
     3721 agatgcacgc aaaggttctt tggcaccgac aaaacttcag cgtgcgacgc aaaaccaaca
     3781 ccgccacacg ccactcgatc gccgacgtga aactcgctca cgtcttctgc caccgccgtg
     3841 actattcccg acgcgctata acccagcgcc tgcgacgcac ccatcttgtc gcgcacagcc
     3901 gcaaaagtat tcagcaagcc ctcactcttc accttcgcaa caaccgcctt caccaaatcc
     3961 ggccgctcgc gcgcttcctg gaccagccct ttgctcacaa gctcaaccgc tgctcgctcc
     4021 gttcccgcac taatcaacga agcaaccgtg cgcaccagca cccggccacg ctgtaacgcc
     4081 ggcaccggca cctctccaac aaacggcctg cgcgtttttc ggttttgcaa aacctgcttc
     4141 acttgtttct ttgtctatct gtgttctttc gtggctaaaa ccttctcaat cagttcactc
     4201 acatactcag cgcgcgcctc ccaggtcccc gacgcaacag cggcctgtct ttgcttgctc
     4261 gcctgcggat cgctttcgtg cagcgcctcg tccaccaacc gaaaaaactc atcacgcgta
//
LOCUS       DASTEY010000649         4815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_526347, whole genome shotgun sequence.
ACCESSION   DASTEY010000649 DASTEY010000000
VERSION     DASTEY010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526347"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VFO72_11300"
     CDS             complement(<1..1444)
                     /locus_tag="VFO72_11300"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HET9479925.1"
                     /translation="MRLLLASLTIAIALQIAPAQQTNPVDRKVTNPITDTPNVNPLTT
                     DQPVQRRPSTQGGVPGVATDQITVDATRQSASGPENARVVVHEGNVDVRIGTYRLQAD
                     KVTVYEAENRVVAEGSVVFDQADQQRITGSRAEWNYRTKTGYFVDSTGFTNQTQDGTR
                     IYFTADRVEKISLDTIVATNVQVTACDEDVPKWSFHASKARIKTGDRVRVYSPNLRLK
                     DIPVFYLPYASVSIKPRDRASGFLTPTFGGSGAKGLRLSTAYYQTLGRSADFTFRSDI
                     YTQRGLGFGGDLRTRANSRSYLNFGFYAVKDRIFGPAEDEAHPDQGGSSLYVEGVHYF
                     KNGFIAASDVNITSNLAFRQVFSDNIQQAISPEETSQVFINKDHDEYSFNFLARTQVT
                     SLTNSRIRIRQLPSISIDKRPTILDFFKDLPVYFSFSGSADGVSRKETVEDLTLFVNE
                     VGGNPIISPSMVQRLDLHPRFELPLNFAGWT"
     gene            1854..3008
                     /gene="lpxB"
                     /locus_tag="VFO72_11305"
     CDS             1854..3008
                     /gene="lpxB"
                     /locus_tag="VFO72_11305"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET9479926.1"
                     /translation="MAKRFVLMIVAGEPSGDAHAAALVNALRKKSPEDEFECFGATGP
                     LMRAAGVETIVDSDELAIMGILEVTRVLPKFLDAFHKLKRAALQRSPDAVVLVDWPEF
                     NLRLATALHRRGLKVIYYISPQLWAWRPRRINTIKRNVDLLLSILPFEAEWFKARGVD
                     HVEFVGHPLAGEVKARFGREEFCRQNDLNPAQPIVSFLPGSRRKELQRILPPMLAAIA
                     RLSAARPDVQCVVVVAPSRTIEETRQIIVQQNGAGAIKLIQKQTREALAASDVAAIAS
                     GTATLEAALLETPMVVVYKESAVNWHTLGRLITVSHFGLVNLVAGREIAKELMQNDLT
                     GANLADELLKLLDPATNKNARTQLAHVAHTLGEAGASERAAELIHHFLSV"
     gene            complement(3005..4027)
                     /gene="lpxD"
                     /locus_tag="VFO72_11310"
     CDS             complement(3005..4027)
                     /gene="lpxD"
                     /locus_tag="VFO72_11310"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET9479927.1"
                     /translation="MKIEELAELVGGGVVGHGEKQIERIGDLERADELEIAYVDNEKF
                     YEAAQNSKAGCLIVPVGVAEKFAGRTVIEVSNPKLAFSLIGAALHPPVRREPEIHSTA
                     VIAENADVALTAYIGPHVQIGAGAHVGASTRIEAGAVVGANVTIGDHCVLQPNVVLYD
                     GVTIGNRVILHAAVCIGADGFGYVRHDHGYQKFPQIGTVVIEDDVELGAHTCVDRAAL
                     GRTRIGRGTKLDNMVHVGHNCDIGERVVIAAQTGISGSVVIEDDAVIGGQVGFGDHTR
                     VQKGAVIGSKAGVLPGKIVRPGVWWGIPIQPLDQYKRMNAYLSRLPERIKKLEDQIAE
                     LKRNTD"
     gene            complement(4024..4797)
                     /gene="lpxA"
                     /locus_tag="VFO72_11315"
     CDS             complement(4024..4797)
                     /gene="lpxA"
                     /locus_tag="VFO72_11315"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET9479928.1"
                     /translation="MIHNRIYPLRHPENSVILSQMAIHPTAIIEDNVEIGPDCELAAY
                     AVIKRFTRLGARNRIYEHAIVGGEPQDVKFNGEQSFLEIGDDNLIREFCTLHRASGEG
                     ETTRIGSRNFFMVGVHVAHNCTIGDDNVFANEVALAGHITIEDHVFLSNNVGAHQFVR
                     MGRYAMVGGKSKIVQDVLPFFITDGNPPRVRGLNTVGLTRAGFSEEAKRALKNAYRTL
                     FKSAAPLVDVLSELEQIDDENVSHLVSFIRSSKRGFIRA"
BASE COUNT         1035 a         1375 c         1357 g         1048 t
ORIGIN      
        1 tggtccaacc ggcgaaattc aacggcaact cgaaacgagg atgcagatcg aggcgctgca
       61 ccatcgacgg cgagatgatc ggattaccgc caacctcatt cacaaacagc gtcaggtctt
      121 ccaccgtttc cttgcggctt acaccgtcgg cgctgccact gaaggaaaag tagaccggca
      181 ggtccttgaa aaaatccagg atcgtcgggc gcttgtcgat gctgatgctc ggcaactggc
      241 gaatgcgaat gcgcgagttg gtaagcgacg tcacttgcgt ccgcgccagg aagttgaaac
      301 tgtactcgtc gtggtccttg ttaataaaga cctgcgaagt ttcttccggc gagatcgcct
      361 gctggatgtt gtcggaaaag acctggcgaa acgcgaggtt cgaagtgatg ttcacgtccg
      421 aggcggcgat gaaaccgttt ttgaagtaat gcacgccctc gacgtaaaga ctcgaaccgc
      481 cctggtcggg atgcgcttcg tcttcggctg gaccaaagat gcgatctttg acggcgtaga
      541 agccgaagtt gagatacgaa cgcgagttcg cgcgcgtgcg caaatcgccg ccgaagccga
      601 gtccgcgctg cgtgtagatg tcgctgcgaa acgtaaagtc ggcggagcgg ccgagcgttt
      661 ggtaataagc agttgagagt cgtagaccct ttgctcctga cccgccgaac gtcggcgtca
      721 gaaatcccga cgcgcgatcg cgcggtttga tcgaaaccga cgcatacggc aagtaaaaaa
      781 cgggaatgtc tttcagtcgc aagtttggcg aatagacgcg gacgcgatcg ccggttttga
      841 tccgcgcttt gctcgcgtgg aaactccact tcggcacgtc ttcgtcgcac gcggtgacct
      901 ggacgttcgt ggccacgatt gtgtcgagac tgatcttctc gacgcgatcc gcggtgaagt
      961 agatacgcgt gccgtcctgt gtctgatttg tgaagccggt cgagtcgacg aagtatcccg
     1021 tcttggtgcg atagttccac tcggcgcgtg aaccggtgat gcgttgctga tcggcttgat
     1081 cgaaaacgac actgccctcc gcgactactc tgttctctgc ttcgtaaacc gtgactttgt
     1141 cggcttgcag acggtaggtg ccgatgcgaa cgtcgacgtt tccttcgtgg acgacaacgc
     1201 gcgcgttttc gggaccggaa gcggattgtc tggtcgcgtc gacggtaatc tgatcggtgg
     1261 cgacacccgg cacaccgcct tgagttgatg ggcgtcgctg aacgggttga tcggtagtaa
     1321 gcggattgac gttcggtgtg tccgtgattg gattcgttac tttacgatcg accggattcg
     1381 tctgctgcgc cggcgcaatc tgcagagcga tggcaatggt caggcttgcc agcaacaaac
     1441 gcatcacggt tgggagaccg cttgagcaaa aacgaaggcg caaacgcgag tagaaccctc
     1501 cgggcgaaag gtcgacaaga gcgcgtggat gctagcatga atgccgacag cgcgcgaatg
     1561 gacgtttggt gcagtcgtgt cctaatctga acgccgaagg cgttatcgaa ttccagccca
     1621 gaggttggag cttgcgacaa ccctggggac tgattggatg tttttacctt tacgctgaaa
     1681 gcgttggcta accgcgagtt tgccaacgcc ttcagcgtta ggacgggacg ctaacgttcg
     1741 acccagggtt gaagcaaccc tgggctggaa ttagcaaacg ccttcggcgt tcagatagga
     1801 caccaccttt ggtgctttgt actttgtgct ttgttctttg tactttgtaa tccgtggcta
     1861 aacgcttcgt actcatgatc gtcgccggtg agccgtcggg agatgctcac gcggccgcac
     1921 ttgtcaacgc gctgcgaaag aagtcacccg aagatgagtt tgaatgtttt ggcgcaacgg
     1981 gtccgttgat gcgtgcggcg ggagtggaaa cgatcgttga ttctgacgaa ctcgcgatca
     2041 tgggcattct tgaagtgacg cgagtgttgc caaagttcct cgatgcgttt cacaaactca
     2101 aacgggcggc gctgcaaaga tcacccgatg cggttgtgct ggttgactgg ccggagttca
     2161 atctacgcct ggcgacggca cttcaccggc gcggcttgaa agtcatctat tacatcagtc
     2221 cacagttatg ggcgtggcgg ccgcgtcgca tcaacactat caaacgcaac gtcgacctcc
     2281 tgttgtcgat acttccgttt gaagcggaat ggttcaaagc acgcggtgtg gaccacgtgg
     2341 agtttgtagg tcacccgctg gccggcgaag tgaaggcgcg ttttggacga gaagagttct
     2401 gccggcaaaa cgatctcaat ccagcgcaac caatcgtctc gtttctgccc ggcagccggc
     2461 gcaaggagtt gcaacgcata ctgccgccaa tgctcgccgc gatcgcaaga ctctcagccg
     2521 cgcgtccgga cgttcaatgc gtcgttgtag ttgcgccaag ccgcacgatt gaagagacaa
     2581 gacagatcat cgtgcaacag aacggcgccg gcgcgatcaa gttgatccag aaacagactc
     2641 gcgaagcact cgctgcatcg gacgtggccg cgatcgcgag tggcactgca acactcgaag
     2701 ccgctctgct cgaaacgccg atggttgtgg tctacaaaga gtcggcagtg aactggcaca
     2761 cactgggccg cctgatcaca gtctcacatt ttggcctggt gaacttggtg gcgggtcggg
     2821 agatcgcgaa agagttgatg cagaacgatc tcaccggcgc gaacctcgcc gacgaactgt
     2881 tgaagttact cgatcctgct accaacaaga acgcacgcac gcagttggct cacgtggccc
     2941 atacgctggg cgaagccggc gcttccgaac gcgccgcgga gttaatccac cattttttat
     3001 ccgtctaatc cgtgttccgc tttaactccg caatctggtc ttcgagcttt ttgatgcgct
     3061 ccggcaagcg actcaaatac gcgttcatac gcttgtactg gtccaggggc tgtatgggta
     3121 ttccccacca aacgccgggc cgcacgatct tccccggcag tactcccgct tttgagccaa
     3181 tcaccgcgcc tttttgcacg cgcgtgtgat cgccaaaacc aacctgcccg ccaatcaccg
     3241 cgtcgtcttc gatcacgacg ctgccgctga tgcccgtttg cgcggcaatc accacccgtt
     3301 cgccgatgtc gcagttgtga ccgacgtgga ccatgttgtc gagtttagtg ccgcggccga
     3361 ttcgtgtgcg gccgagagcc gcgcgatcga cgcacgtgtg cgcgccgagt tcgacatcgt
     3421 cttcgataac gaccgtgccg atctgcggaa acttctggta accgtgatcg tgccgcacgt
     3481 agccaaaacc gtcggcgccg atacagacgg cggcatgcag gatgacgcga ttgccgatcg
     3541 tgacgccgtc gtaaaggaca acgttcggct gcagcacaca gtggtcgccg atcgtcacgt
     3601 ttgcgccaac tacagcgccg gcttcaatcc gcgtggacgc accgacgtga gcgcctgcgc
     3661 cgatctgaac gtgtggaccg atgtatgccg tcagcgccac gtccgcgttt tcagcgatga
     3721 ccgccgtcga atgtatttca ggttcacggc ggaccggcgg gtgaagcgca gcgccgatca
     3781 gcgagaaggc aagcttcgga tttgaaactt caatcaccgt ccgtcccgca aacttctccg
     3841 ccactccaac cggcacaatc aaacacccgg ccttactatt ctgcgccgct tcataaaact
     3901 tctcattatc aacgtacgca atctccagct cgtccgcccg ctccagatct ccaatccgtt
     3961 caatctgttt ttctccgtgg cccacgacgc cgccaccgac gagttcagca agctcttcta
     4021 tcttcatgct cttataaacc cacgtttgga actacggatg aaactgacca aatgagaaac
     4081 gttttcgtca tcgatctgct cgagttcgct taacacatca accagcggcg ccgccgactt
     4141 gaacaacgtg cgatacgcat tcttcaacgc ccgtttcgct tcttccgaaa acccggcgcg
     4201 cgttaacccg acggtgttga gaccacgcac acgcggcgga ttgccgtcag taataaagaa
     4261 cggcagcaca tcctgcacga tcttcgactt cccgccaacc atggcgtaac ggcccatccg
     4321 cacaaactga tgagcgccca cgttgttgga aagaaaaacg tgatcctcga ttgtgatatg
     4381 gcccgcgagc gccacttcat tcgcaaagac gttgtcgtcg ccaatcgtgc agttgtgcgc
     4441 aacatgtacg ccgaccatga agaagttgcg cgaaccaata cgcgtagttt caccttcgcc
     4501 gctggcccgg tgcagcgtgc aaaactcgcg aatcagatta tcgtcgccga tttcgagaaa
     4561 actctgctcg ccgttgaact taacgtcctg cggctcgccc ccgacgatgg catgctcgta
     4621 aatgcgattt cgggcgccta accgcgtaaa acgcttgata acggcgtacg cggcaagctc
     4681 gcaatctggg ccaatttcga cgttgtcctc gattatggcg gtcggatgaa tagccatttg
     4741 tgacaagatt acagaatttt caggatgtct caacgggtaa atcctgttgt ggatcatgta
     4801 aatcctgttc tatga
//
LOCUS       DASTEY010000650         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_607868, whole genome shotgun sequence.
ACCESSION   DASTEY010000650 DASTEY010000000
VERSION     DASTEY010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607868"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VFO72_11320"
     CDS             <1..1287
                     /locus_tag="VFO72_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9479929.1"
                     /translation="TAVIFTLLPALKASRPDINEVLKAGAKSATSSSSLWRDSLVVAE
                     VALGLVLLIGAGLMMRSFASLTNVHPGFDPQNVLTARITLSGPAYEDTDARKRYVSQT
                     IERLKALPGVESAALVAPMPFSGAEIGGDFKIEGQNTEPGREPAANVRNVTPEYFQTI
                     RIPLLKGRYFNEQDQRGAVGAAIVNERFARRYFINDDPLGKRIKDLGVNQNEGDPKQY
                     EIVGVVGDVHHNSLIRSATPELYLPHQQNSWTWGNFLVRTTNDPATLTRSFTDTIRST
                     DKTVPVTRVRPLTEAISETISQPRFYALLFGLFGATGLLLTLTGIYSVISYTVSNHTR
                     EIGIRMALGAQGRDVLKLIVGKGLVLTLIGIGIGLLGAFGITRVMQTLLFGVSATDWV
                     TFTAVAMLLALVGLLATAIPARRATKVDPLVALRYE"
     gene            1308..>2516
                     /locus_tag="VFO72_11325"
     CDS             1308..>2516
                     /locus_tag="VFO72_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479930.1"
                     /translation="MKIVILGLLLTSFAFQQQAPMFVSNNPQSIYAADPNDSWNRIFR
                     ALFTRTVKARVSDAFPEGAPFSPFHIRMGSFSIRLSKEKISRTEIGDRAIEPLYPTFL
                     TATGPLQVFSEPLFTELTAALREAINETKTRSPIERALMQADVWAAYDILYSMRDAVS
                     PERSTKLLSLLRQFLRKLALSNDEIKLLKSNYVAVASLPNLFSTDSGWLEIELLPDRH
                     HDFVASYRRAARVFVKPRTPPADAGQFVESLKHNQHHDRVEAVALVVQNLLIDTSGRV
                     VPSPLFSDVQFRFFKNDATTGVTSAEPQQFELSRRKLLTEPVSGGFVEFAATYLSGAG
                     NDYDFATPIEDAEAPVLVPLRSRCTQCHNRSLTTMMTYSIHYFPPVPTVRILRSSDQE
                     RAFHVARRKED"
BASE COUNT          574 a          669 c          681 g          592 t
ORIGIN      
        1 actgccgtta tcttcacttt gctgccggca ttgaaagcct cgcgtcctga catcaatgaa
       61 gttttaaaag ccggagcgaa gagcgcgacg agcagtagtt cgttgtggcg tgattcgtta
      121 gtcgtcgcgg aagtcgcgct cggcctggtg cttctcatcg gcgcaggcct gatgatgcgc
      181 agcttcgctt cgctgacgaa cgtgcatccg ggttttgatc cgcagaatgt tctgacagca
      241 cgcattacgc tcagcggtcc tgcttacgag gacacggacg cgcgcaagcg ttacgtcagc
      301 caaacgattg agcgactcaa agctctgccg ggcgtcgaga gcgctgcgct cgtcgcgcca
      361 atgccattca gcggcgctga aatcggcggc gactttaaga tcgaaggtca gaacaccgaa
      421 cccggacgag agccggcagc caacgtgcgc aacgttacgc cggaatactt tcagaccatc
      481 agaattcctt tgctgaaggg acgctatttc aacgaacagg accaacgcgg cgccgtcggc
      541 gctgctatcg tcaatgagag gttcgccagg cgttatttca taaacgacga cccgcttggc
      601 aagcgcatca aggacctggg cgtgaatcag aacgaaggcg atccgaaaca atatgagatc
      661 gtcggtgttg ttggtgatgt gcatcacaac agtttgatca gatctgcgac acccgaactc
      721 tacctgccac atcaacagaa cagttggacc tggggaaatt tcctcgtgcg cacgacaaac
      781 gatccagcaa ctctgacgcg aagtttcact gacaccattc gctcgactga caagaccgtg
      841 ccggtgacgc gggtcagacc gctgacggaa gcaatctcag aaacgatttc ccaaccacgg
      901 ttttatgcgt tgctgtttgg attgtttgga gcaaccggct tgctactgac tttgacgggc
      961 atttacagcg ttatctcgta cacggtctcg aatcacactc gcgagatcgg aatcagaatg
     1021 gcgctcggcg cacaaggtag agacgtgttg aagttgatcg tcggaaaagg cctggtgttg
     1081 acgttaatcg gtatcgggat cgggctgctt ggcgcgttcg gcatcacgcg cgtgatgcaa
     1141 acgttactgt tcggcgtaag cgcgactgac tgggtcacgt tcactgccgt agcgatgttg
     1201 ctcgcgctcg tcggtcttct cgcaacggcg attcccgctc gccgcgcaac gaaagtcgat
     1261 ccgctggtgg cgctgagata cgagtagcga agttaacttg cgtaactatg aaaatcgtca
     1321 ttcttggtct attgctcaca tctttcgcct ttcaacagca ggcgccgatg tttgtctcca
     1381 acaatccaca gtcaatttat gcggcagatc cgaatgactc atggaatcga atcttccgcg
     1441 cgttgtttac gcgcactgta aaggctcgcg tgtcagacgc ttttcccgaa ggtgctcctt
     1501 tcagtccgtt tcacatcaga atgggctcct tctcgatacg tcttagtaaa gagaagatta
     1561 gtcgaacgga gatcggcgac cgcgcgatcg aaccgcttta cccaaccttc ttgactgcca
     1621 caggaccttt acaggttttc tctgagccac tcttcactga attaactgct gcccttcgtg
     1681 aagcaatcaa tgaaacgaag acgcgatcgc cgattgagcg cgcgctgatg caggccgacg
     1741 tttgggcggc ttacgacatc ttgtattcaa tgcgcgatgc agtaagtccc gaacgcagta
     1801 caaagcttct cagtctcttg cgacagttct tgcgaaagct ggcgttatcg aatgatgaga
     1861 tcaaattact caagagtaac tacgtcgccg tagccagtct gccgaatctg ttttcaacag
     1921 acagcggatg gcttgaaatc gagttgctgc ctgatcgtca tcacgacttc gtggcgagtt
     1981 atcgtcgtgc tgcacgagta tttgtgaagc cgcgaacacc accggcggac gcgggccaat
     2041 tcgtggaaag cctcaagcat aatcaacacc acgatcgagt ggaggccgtc gcgcttgttg
     2101 tgcagaactt gttgatcgat acatcgggtc gagtcgttcc ttcaccgttg tttagcgatg
     2161 ttcagttccg gtttttcaaa aacgacgcaa cgactggtgt tacttccgcg gaaccgcaac
     2221 agtttgagtt gagccgaaga aagttattga cggaaccggt gtcaggcggg ttcgtcgagt
     2281 ttgcggccac atacctgagc ggtgcgggaa atgattatga ttttgccacg cccattgaag
     2341 atgctgaggc gccagtactg gtgccgttgc gtagtcgttg tacacaatgt cacaaccgtt
     2401 cgctaacgac gatgatgact tactcgatcc attactttcc acccgttccc accgtcagga
     2461 ttctgagatc gtcggatcaa gagcgcgcat ttcacgtcgc gcggcgtaaa gaagat
//
LOCUS       DASTEY010000651         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_427023, whole genome shotgun sequence.
ACCESSION   DASTEY010000651 DASTEY010000000
VERSION     DASTEY010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427023"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VFO72_11330"
     CDS             <1..1093
                     /locus_tag="VFO72_11330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET9479931.1"
                     /translation="LCKNSHKIENSYIVVLDDTVVGERGRFSIAPFIAAELATTHRGN
                     LKHVYQNALNGFAVEMSAEDAERLSQDFRVKYVEEDGVVTLDATQSNPPWGLDRIDQR
                     NRPLSGTYTYNRTGSGVRAYVIDTGILTSHTQFGGRAQNVFDAFGGNGTDCNGHGTHV
                     AGTIGGSTYGVAKSSLPRGVRVLNCSGSGSNSGVIAGVDWVRQNFIAPAVANMSLGGG
                     ASSALDTAVNNLSNAGVAIAVAAGNSNTNACNSSPARAANAITVGATTSTDSRASFSN
                     FGSCLDLFAPGQGILSAWIGSTSATRTISGTSMASPHVAGVAALYKQANPSASASTVR
                     NAIVNNATTNVVTNAGTGSPNRLLYSLFF"
     gene            1197..4154
                     /locus_tag="VFO72_11335"
     CDS             1197..4154
                     /locus_tag="VFO72_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9479932.1"
                     /translation="MKLQRSVSFLCTFALLTTIAVAQQAAAPATKRALTHSDYDSWRS
                     ILAHQVSRDGKFVAYAYVPQDGDGEIVVRNIATGVDWRAPRGYRPPVPPPDDPGTNLA
                     EYQAQQARLLRPVFTADSRFLVFSTEPMKAELNKAKKEKRKPEDMPKNGLSIMDLSTG
                     TVTRIERVKTFRVPEDGSGFIAYLLEPRLASNASPTGEPKPATPATETPTPTPRPQGG
                     GRPAKKKEYGSELVLLNTVTGNERKFDDALDFTISKDARTLIYTVSSRKEETNGVYAV
                     TTQSDIAPIALLSGKGKYQRLTWDEDNTQIAFISDKEDAAAKQPKFRVYHWNLKDPQA
                     TEIVSPTSAGFSKDMVISERGNLSFSLDGSRLFLGSAPPPEPEKNPDEEIPADEKVLV
                     DLWHWKDDYVQPIQKVRAEQDRNRSYRAVYNLESKKFVQLADETMENASPSNNGRFAI
                     GSDNRKYRVTNDYDPGYTDYYLVDTADGSRKPLATRQRGGYSLSPNAKYALYFDGKDW
                     NTYAIADGTVTNLTKNLKVNFYNEQHDSPSIPGSYGVAGWTKDDNDVLIYDRFDIWQI
                     APDGSRAKNLTDGVGRKEKTTLRYVRLDPREHWIDPDKPLLLSAESQETRDSGFYRDK
                     VNSDALPQKLLMAAKDFNTPTKAKNADVLIMTASRFDQFPDIWVTNSTFRELKRVSNG
                     DAQRANYLWGTAELVSFKNVDGVPLKGVLLKPENFDPRKKYPMIVYIYERLSQGLHQF
                     RNPGPGTSINPSFYVSNGYLVYMPDIVYTIGYPGSSALKSVLPGIQAVVDKGFVNEDA
                     IGIQGHSWGGYQIAYLVTQTNRFKAAAPGALVANMTSAYSGIRWGTGLPRQFQYERTQ
                     SRIGGSLWEYPLRFLENSPIFRADRVQTPLLMLHNDEDDAVPWYQGIEYFLALRRLGK
                     EAYMFNYNGEKHGLRKRINQKDYTRRLQEFFDHFLKGAPAPEWMEKGIPYLQREKEKE
                     KYRISEEDTKPTP"
     gene            complement(4196..>4988)
                     /locus_tag="VFO72_11340"
     CDS             complement(4196..>4988)
                     /locus_tag="VFO72_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET9479933.1"
                     /translation="NYDIIVDGTDNFPTRYLVNDACVLSGKPNVYGSIFRFEGQASVF
                     WAEKGACYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGAIQANETIKIILGAEGLLIN
                     RLLLFDAWPMKFREFKLRKDPKCPVCGDNPTITKLIDYEEFCGVAPHQLPPSDPKLEE
                     ITATELSARRLREDKIQIIDVREPHEFEIARIPGTKLIPLGHVVTRMNEIDPEVETIV
                     HCKGGVRSAKAIEALKRAGFQGRLINLKGGITAWSEDVDPSVAKY"
BASE COUNT         1172 a         1472 c         1348 g          996 t
ORIGIN      
        1 actgtgcaag aactcacaca agatcgagaa cagttacatc gtcgtgctcg atgataccgt
       61 tgtcggcgag cgtggtcgtt tctccatcgc tcccttcatc gcagccgagt tggctacaac
      121 ccaccgcgga aatctcaaac atgtctacca gaatgctctc aacggcttcg cagtcgaaat
      181 gtcggccgaa gacgcagagc gtttgagcca ggatttcaga gtcaaatatg ttgaagaaga
      241 cggtgtggtt acgcttgatg caactcagag caacccaccg tggggcctgg atcgcatcga
      301 ccagcgcaac cggcccttga gtggcaccta cacttacaac cggaccggtt ccggcgttcg
      361 cgcttacgtg atcgacaccg gtattctcac cagccacact cagtttggcg gacgtgctca
      421 gaacgtgttt gacgcctttg gcggaaacgg tacggattgc aacggtcacg gcacacacgt
      481 ggctggaaca atcggtggtt ccacctacgg cgtggcgaag agctcgttac cgcgtggtgt
      541 gcgcgtgctc aactgttccg gatccggatc aaactcgggc gtgattgccg gcgtcgattg
      601 ggtcagacag aacttcatcg caccggccgt tgcgaatatg agtctcggtg gcggcgcttc
      661 gagtgcgttg gacaccgcgg taaataacct ttcgaatgct ggggtcgcga ttgcggttgc
      721 ggccggtaac agcaacacga acgcctgcaa ctcgtcgccg gctcgcgcgg caaacgcaat
      781 tacagtcggt gcaaccacct cgaccgactc acgtgcgtcg ttctcgaact tcggttcctg
      841 cctcgatctc ttcgcgcccg gtcagggtat tttgtcggcg tggattggct cgaccagtgc
      901 cactcgaacg ataagtggaa cgtcaatggc ctctccacac gtcgcgggcg tggcagcgct
      961 ctacaagcag gcgaatccgt cggcatcggc ttcgactgtt cgcaatgcga ttgtcaacaa
     1021 tgcgacgacc aacgttgtga ccaacgccgg caccggttcg ccaaaccggc tgctgtattc
     1081 gttgttcttc tgattggtct ttactttggt gcagattgac gcgggtgaca cggatctgtt
     1141 ttgatctgcg tcatccgcgt ttgtctttgt aatcccacaa cctgcgaggt aaacccatga
     1201 agcttcagcg aagtgtttct ttcctttgca cgtttgcttt gctaactacg atagccgtag
     1261 cgcaacaagc ggcggcgccc gcgaccaaac gagcactcac tcacagcgac tacgattcgt
     1321 ggcgatcaat cctggcacac caggtgtcgc gtgacggcaa attcgtcgcc tacgcgtacg
     1381 tgccgcaaga cggcgacggc gagatcgtcg tgcgtaacat cgccaccgga gtcgactggc
     1441 gcgcgccgcg cggttaccgt ccgcccgtgc cgccaccgga cgatcccggc accaacctcg
     1501 cagagtatca ggcacaacag gcgcgcttgc tgcgtcccgt tttcaccgcc gacagccgtt
     1561 tcctcgtctt ctccaccgaa ccaatgaaag cggaacttaa caaagcgaag aaagagaaac
     1621 gcaaacccga agacatgccg aagaacggtc tcagcatcat ggacctctcg acgggaacgg
     1681 tgacgcgcat cgaacgagtg aagacgttcc gcgtaccgga agatggatct ggttttattg
     1741 cttatttact cgaaccgagg ctcgcctcca acgcttctcc tacaggcgaa ccaaaacccg
     1801 caactccagc taccgaaacc ccaacgccta cacctcgtcc gcaaggcggc ggccgtccgg
     1861 cgaaaaagaa agagtacggc tcagaactgg ttctgcttaa caccgtcacg ggcaacgaac
     1921 gaaagttcga cgacgcgctc gacttcacga tcagcaaaga cgccaggacg ctcatctaca
     1981 cagtttcatc acgcaaggaa gaaacaaacg gcgtctacgc agtcacgacc caaagcgaca
     2041 tcgcaccaat cgctttactg agcggcaaag gaaaatacca gcgcctcacc tgggacgaag
     2101 acaacacgca gatcgctttc atcagcgaca aggaagacgc cgcggcaaaa cagccgaagt
     2161 tccgcgtcta tcactggaac cttaaagatc ctcaagcgac ggaaatcgtg tcgcccacgt
     2221 cggccgggtt ttccaaagac atggtgatca gcgagcgcgg caatctcagc ttctcgctcg
     2281 atggtagtcg cctgttcctc ggctcagcgc cgccgcccga accggagaaa aaccccgacg
     2341 aagagattcc ggcggatgag aaggtgctcg tcgatctgtg gcattggaaa gacgattacg
     2401 tgcagccgat tcagaaagtg cgcgccgagc aggaccgcaa tcgctcgtat cgcgcggtct
     2461 ataatctgga atccaaaaag ttcgtccaac tcgccgatga aacgatggag aacgcgtcgc
     2521 cgtcaaacaa cggccgcttc gcgatcggct ctgacaaccg caagtatcga gtgacgaacg
     2581 attacgatcc cggctatacc gattactacc tcgtcgacac cgccgacggt tcgcgcaagc
     2641 ccctggcaac cagacaacgc ggcggatact cgctgtcgcc aaatgcgaag tatgcgctct
     2701 acttcgacgg caaagattgg aatacgtacg cgatcgctga cggaacggtc acgaatctca
     2761 cgaagaacct caaagtcaac ttttacaacg aacagcacga cagtccttca atccccggct
     2821 cgtatggcgt ggctggatgg accaaagacg acaatgacgt actcatttac gatcgttttg
     2881 acatctggca gatcgcgccc gacgggagcc gcgccaaaaa cctgactgac ggcgtcggcc
     2941 gcaaagaaaa aacaacgctg cgctacgtgc gactcgatcc acgcgagcac tggattgatc
     3001 cggacaaacc gctattgctg agcgccgaga gccaggagac gcgcgactcg ggcttttatc
     3061 gtgacaaagt caacagtgac gcactgcctc agaagctcct gatggccgca aaagacttca
     3121 acacgccgac taaagcgaaa aacgccgacg tactgatcat gactgcctcg cgtttcgatc
     3181 agtttcctga catttgggtg accaacagca ctttccgcga gctgaagcgc gtcagcaacg
     3241 gcgacgccca acgcgctaat tacctgtggg gcacagcaga gttggtgagt ttcaaaaacg
     3301 tggatggcgt gccgttgaaa ggcgtgttac tgaaaccgga aaacttcgac ccgcggaaga
     3361 agtatccgat gatcgtttac atttacgagc ggctgtcgca gggcttgcac cagttccgca
     3421 atcctggacc agggacgtct atcaatcctt cgttctatgt gagtaacggc tacctggtct
     3481 acatgcctga catcgtctac acaatcggat atccgggaag cagcgcgttg aaaagcgtgt
     3541 tgccgggaat tcaggcggtg gtcgataaag ggtttgttaa tgaagacgcg atcgggattc
     3601 agggacacag ttggggcggt tatcagatcg cttacctggt gacgcagacc aatcgcttca
     3661 aggcggccgc ccccggcgcg ttggtggcaa acatgacgag tgcttacagc ggaattcgct
     3721 ggggcacggg cttgccgcgc cagtttcagt acgagcgtac gcagtcgcgc atcggcggca
     3781 gcttatggga gtacccgctg cgtttcctcg agaactcgcc catcttccgc gcagaccgcg
     3841 tccagacgcc gctgcttatg cttcacaacg atgaagacga tgcagtgccg tggtaccagg
     3901 gcatcgaata cttcctcgcg ctacgccggc tgggtaaaga agcttacatg ttcaactaca
     3961 acggtgagaa gcacggcctg cggaagcgaa tcaaccagaa ggactacaca cgccggttgc
     4021 aggaattttt cgaccatttc ctgaaaggcg cacccgcacc ggagtggatg gagaaaggca
     4081 ttccctacct ccaacgcgag aaagagaaag aaaaataccg catcagcgaa gaagacacta
     4141 aacctacacc ttaacctgac ttagctcagt ccgtatcatc cgtatcatcc gtggctcagt
     4201 acttcgccac gctcggatcg acgtcttcag accacgctgt aatgccgcct ttcaagttga
     4261 tcagacggcc ctgaaatccc gcacgcttaa gggcctcgat cgcttttgcg ctccgcacgc
     4321 cacctttgca atgcacgatc gtttcgactt cgggatcgat ttcattcatg cgcgtcacga
     4381 cgtgtccgag cggaatcagt ttcgtcccag gaatgcgcgc gatctcgaat tcgtgcggct
     4441 cgcgcacgtc gatgatctga atcttgtctt cacgtaaacg gcgcgcgctc aattcggtcg
     4501 cggtaatctc ttcgagtttt ggatcggagg gtggaagctg gtgcggcgcg acgccacaga
     4561 actcctcgta gtcgatcagc ttcgtgatcg tcggattgtc gccgcacacc gggcacttcg
     4621 gatcctttct cagtttgaac tcgcgaaact tcatgggcca ggcgtcgaac aacagcagtc
     4681 gattgatcag gagaccttcc gctcccagaa tgatcttgat tgtttcgttt gcctgaatcg
     4741 cgccgactat gccgggtaaa acaccgagca cgccgccttc cgcgcacgac ggaacgagtc
     4801 cgggcggcgg cggttcggga tacaaacagc gataacacgc gcccttctcc gcccaaaaca
     4861 cgctcgcctg tccttcaaac ctgaagatcg aaccgtagac gtttggcttg ccgctcaaca
     4921 cacacgcatc gttgacgaga tagcgtgtgg gaaaattgtc agtgccatcg acgattatgt
     4981 cgtagttg
//
LOCUS       DASTEY010000652         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1152312, whole genome shotgun sequence.
ACCESSION   DASTEY010000652 DASTEY010000000
VERSION     DASTEY010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152312"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..500
                     /locus_tag="VFO72_11345"
     CDS             63..500
                     /locus_tag="VFO72_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9479934.1"
                     /translation="MTTQAVASRDERGQPTLVNGHRTNVNKDNWLGFVGDVVNFTGEV
                     CFKSMLRIDGHFSGNVTSPDGTLIISTGAQVTKAVIKVAVAKINGTVEGDILASKQVV
                     LGRTANVRGHLAAPALVVEEGAQLNGSCRRIEANAAELISNRR"
     gene            complement(480..1343)
                     /locus_tag="VFO72_11350"
     CDS             complement(480..1343)
                     /locus_tag="VFO72_11350"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9479935.1"
                     /translation="MTIQIRLMQPEDVETCGRICYEAFKGIAEKHNFRPDFPAPEVAI
                     EVMRMFQERTGVFSIVAESDGQVIGSNHLGEYDAIRSVGPITIDPIAQAKGTGRMLME
                     AVIERGQGAPGIRLVQDSFNVASLSLYASVGFDVREPLVMIEGFVKGDVPAGFEVRPL
                     EKADYESCAELCRSVHGFDRLNELTHTPPMPHPFVAVRDGHITAYISAPQFWPLSHAV
                     AETEQDMRALLLGAGNQCEEPFSFLLPTRQAALFRWLLKNGMRVVKPMTLMSMGEYHE
                     PRGCYLPSVGY"
     gene            complement(1348..2697)
                     /locus_tag="VFO72_11355"
     CDS             complement(1348..2697)
                     /locus_tag="VFO72_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9479936.1"
                     /translation="MRKLWPVLLAITLFATLSDISAQSQANVKALVGGTLIDGYGSKP
                     LRNSVVIIEGERIKAVGQVGTLAIPRGAEVISTEGMTVLPGLWDMHVHLMINGHSDYA
                     HWDKTYPPQMESVIMPASAKQLLMAGITSVRDLGAPLKASIAVRDRIEKGEIPGPTVY
                     VSGPFIQHEPYPGTDYVRWGVKGVEDARAKVRMLADAGVDVIKLIDQDLMTMDEVRAV
                     VDEAHKRKLPVVAHSHRPEEIRRGLLVGVDCFEHTGLATAPEYPADVIAMIRERTAKM
                     SLGPLFWTPTVQGLLNYEYVRDNPEQLDDPSWQVGLPQSIIDDIKGSLKNPDRLSYYQ
                     ITPLRRPTLARKIQQLSESGVVLLIGTDSGIPMSFHSQTTWRELDAWVNSFGIDPLVA
                     IRAATYWPSVAMRVSDQVGTVSEGKYADIIAVRGDVLRHIDLLQNVDIVIKHGRRWK"
     gene            complement(2713..3234)
                     /locus_tag="VFO72_11360"
     CDS             complement(2713..3234)
                     /locus_tag="VFO72_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479937.1"
                     /translation="MKRLLICLSVLMVSSFLIPARGLAQSKTAVQPTPEQSLQELVAE
                     VRQLRATLQRMNAAVYKGQVILERLKLQHEQVTRVSRELRDGRDSLSELRAQQSRMKE
                     MLNRVETDIARGLQRDEDRSNLKAEIGMLSQREHRMLIREAQLASELRIGQSKLDVLN
                     HQLLLVEQEMSQR"
     gene            complement(3250..>3437)
                     /locus_tag="VFO72_11365"
     CDS             complement(3250..>3437)
                     /locus_tag="VFO72_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479938.1"
                     /translation="QGKNCPELWTDHPTAVTSLNWKDRKKTVSHYHGCRGTAVLEQLT
                     KLENKIDEVVNTNRWIK"
BASE COUNT          749 a         1035 c          892 g          761 t
ORIGIN      
        1 ggcggctccg atcccaccta accgtaatcc acgagtgaca ggaaagagaa agaagggcag
       61 cagtgactac tcaagcagtt gcgagcagag acgaacgcgg ccagcctacg ctcgttaatg
      121 gccaccggac gaacgtcaac aaggacaatt ggcttggttt cgtaggtgac gtggtcaact
      181 tcaccggcga agtctgtttc aagtcgatgc tgcgaatcga cgggcacttt tccggcaacg
      241 tcacttcacc ggacggcaca ctgatcatca gcacgggcgc acaagtgacg aaagcagtaa
      301 tcaaagttgc ggtcgcgaag attaacggaa cggtcgaagg cgacatcctc gcgagcaaac
      361 aagtggtact cggccgcacg gcgaacgttc gcggccacct cgccgccccc gccctcgtcg
      421 tcgaagaagg cgcacaactc aacggcagtt gccgacgaat tgaggcgaac gctgcagaac
      481 taatatccaa cagacggtag ataacaaccg cgcggttcgt ggtattcgcc catcgacatt
      541 agcgtcatcg gcttaactac ccgcatccca tttttcaata accaacgaaa gagcgcggct
      601 tggcgtgtcg gaagtaagaa cgagaacggc tcttcgcact gattgccggc gccgagcagg
      661 agtgcgcgca tgtcttgttc cgtctcggca acagcgtggc tcaatggcca gaattgtggt
      721 gcggagatgt acgcagtgat gtgtccatca cgcaccgcga cgaaagggtg gggcattggc
      781 ggcgtgtgcg tcagttcgtt gagacggtcg aagccatgga ctgaacgaca caactcagcg
      841 caactctcgt aatcggcttt ttccagtggc cgcacctcaa atcccgcggg cacgtcgcct
      901 ttcacaaagc cttcgatcat cacgagtggt tctctcacat cgaaacctac cgatgcatac
      961 agcgaaagag acgcaacatt gaaagagtcc tggaccagac ggatccctgg agcaccctgg
     1021 ccacgttcaa taactgcctc catcaacatc ctgccagtcc ctttcgcctg cgcgatcgga
     1081 tcaatcgtaa tcggcccgac cgagcgaatc gcgtcgtatt ccccaaggtg gttcgatcca
     1141 atcacttgcc catcgctctc cgcgacaata ctaaacacac ctgtacgctc ctgaaacatt
     1201 ctcatgactt cgattgctac ttcgggagcc ggaaaatcag ggcggaagtt atgcttctcg
     1261 gcgatgccct tgaacgcctc gtaacagatc ctcccacacg tttcgacatc ttcgggttgc
     1321 attaagcgta tttggatggt catcacgtta tttccacctt cgtccatgct tgatcacgat
     1381 gtctacgttt tgtaacagat cgatgtgccg caacacatcc ccacgaaccg caatgatgtc
     1441 tgcgtacttt ccttcactca cggttcccac ctggtccgat acgcgcatag ccactgacgg
     1501 ccagtaagtt gccgcgcgga tggcgaccag cgggtcgatg ccgaagctat taacccacgc
     1561 gtcaagttcg cgccaggtgg tttggctgtg aaagctcatc ggaatgccgc tgtcggtgcc
     1621 aatcaacagc acaacgcctg actcggacag ttgctggatc ttccgagcca gtgtgggacg
     1681 tcgcaatggc gtgatctggt agtaggaaag tcgatcggga ttcttcaacg accctttgat
     1741 gtcgtcaatg atcgactgcg gcagaccaac ttgccacgac ggatcatcaa gctgctccgg
     1801 attgtcgcga acgtactcgt agttcaaaag tccctgcacc gtcggcgtcc agaacaatgg
     1861 tccaagactc atcttcgccg ttcgctcgcg aatcattgca atgacatccg ctggatactc
     1921 cggcgccgtc gccagtcccg tgtgctcgaa acaatcaaca ccaaccaaca gcccgcgacg
     1981 aatctcctcc ggacggtgcg agtgcgctac taccggcaac ttgcgtttgt gtgcttcatc
     2041 aacaacagcg cgcacctcgt ccatcgtcat caggtcctga tcgatcaact tgataacatc
     2101 cacgcccgcg tccgccagca ttcgcacctt cgcgcgcgca tcttccacac ctttcacgcc
     2161 ccaacgcacg taatcagttc cgggatacgg ttcgtgctga ataaacggac ccgaaacgta
     2221 gacggttgga ccgggaattt ctcccttctc aatgcgatcg cgtactgcaa tactcgcctt
     2281 cagcggcgca ccgaggtccc gcacactagt gatgcccgcc atcagcaact gcttcgctga
     2341 tgcaggcatg atcaccgact ccatctgcgg cggatacgtc ttgtcccagt gcgcgtaatc
     2401 gctgtggccg ttgatcatca ggtgaacgtg catgtcccac aacccgggca acacggtcat
     2461 gccttcggtc gagatgactt cggcgccgcg cggaattgcg agtgtgccga cctggcccac
     2521 agccttaatc cgctcgcctt cgatgatcac gacgctgttg cggagcggct tgctgccgta
     2581 gccgtcgatc agcgtgccac cgacgagtgc tttgacattc gcttgcgatt gcgccgagat
     2641 gtcactaagg gtcgcgaaga gagtgatcgc cagcagaaca ggccacagtt ttctcatgca
     2701 atactcccgg cgttagcgtt gtgacatttc ctgctcgacc aacaacaatt gatggttgag
     2761 cacgtcgagt ttcgactgcc cgatcctcaa ctcgctggcg agttgtgctt cgcgtatgag
     2821 cattcgatgt tcacgctggc taagcatgcc gatctccgct ttcaggttgg acctatcctc
     2881 gtcacgctga agaccacgtg caatgtcggt ctcgacgcga ttcagcattt ctttcatcct
     2941 ggattgctgt gcgcgaagtt cactgagact atcccgcccg tctcgcaact cgcgcgagac
     3001 gcgcgtcacc tgctcgtgct gcaacttcag ccgttcaagt atgacctgac ctttgtaaac
     3061 ggccgcgttc atacgttgca gtgtcgcgcg cagctgccgc acttccgcca ctaactcttg
     3121 cagcgattgc tccggtgtcg gctgtacggc agtttttgac tgggccaatc cgcgcgcggg
     3181 tatcaagaac gacgagacca tcagaactga caaacagatg agcagacgtt tcatggtcgt
     3241 tatctccttc tatttaatcc agcggttcgt attcacgact tcatcgatct tgttttcgag
     3301 cttcgtgagt tgctctaaga ccgcggtgcc gcgacagccg tgatagtggc tcacggtttt
     3361 ctttctgtct ttccagttga gagaagtgac ggcggtggga tggtcggtcc acaattccgg
     3421 acagtttttt ccctgcc
//
LOCUS       DASTEY010000653         6275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1234945, whole genome shotgun sequence.
ACCESSION   DASTEY010000653 DASTEY010000000
VERSION     DASTEY010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234945"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..953
                     /gene="rpsB"
                     /locus_tag="VFO72_11370"
     CDS             75..953
                     /gene="rpsB"
                     /locus_tag="VFO72_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9479939.1"
                     /translation="MKELLEAGVHFGHQVRRWNPKMKEYIFGERNGIYIIDLQKTQRM
                     FREAIAFVTNLIAEDKGRTVLFVGTKRQAQDAIREEAEKCGQYFVNQRWLGGLLTNFQ
                     TVQKSIKRLKDLESMQTDGRYEKLTKKERIKLDRERESLNKNLSGIKMMSRLPDAVFI
                     IDVKKEEIAVAEANRLGIPIVAVVDTNCSPEGIDYVIPGNDDALRAVRLFASRIADAI
                     VEGNQIATEGGVVTTEPIEEPEEGAGEAAAAEFAPTGAEAVNGEEAQSESTTTAGATE
                     TDVARDESPESVVAAS"
     gene            1056..1718
                     /gene="tsf"
                     /locus_tag="VFO72_11375"
     CDS             1056..1718
                     /gene="tsf"
                     /locus_tag="VFO72_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9479940.1"
                     /translation="MAEITAAAVKQLREKTGAGMMECKNALVEAGGNEDKAIDILRER
                     GLASAKKREGRIAAEGIVGSYIHMGGKVGVLVELNCETDFVARGEEFQQLVKDIAMHI
                     AAAEPRYVSREEVPESVLEKEREIARAQAKNDPKMANKPEQVIEKIVEGRLNKFYEET
                     VLSDQPFVKDPSKTVAELVTEKVAKTGERIAIRRFTRYKMGEGLERRADDFGGEVASM
                     LR"
     gene            1715..2443
                     /gene="pyrH"
                     /locus_tag="VFO72_11380"
     CDS             1715..2443
                     /gene="pyrH"
                     /locus_tag="VFO72_11380"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9479941.1"
                     /translation="MKNEASNLAYRRVLLKISGEALMGEQNYGIDVDVARSVAEELKA
                     VHQLGVQVAVVVGGGNIFRGVSKSAGNMDRSSADYIGMLATVMNAVVLQDALEKIEVD
                     TRVLSAVDIPQLAEPFIRRRAVRHLEKGRVVIFAAGTGNPYFTTDSAAALRALEIRAD
                     IILKATKVDGVYSADPITDATAELFNEISYRQVLERELKVMDASAISLCMDNNLPIIV
                     FNMRQRGNIKRVVMGESGVGTKVG"
     gene            2506..3066
                     /gene="frr"
                     /locus_tag="VFO72_11385"
     CDS             2506..3066
                     /gene="frr"
                     /locus_tag="VFO72_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9479942.1"
                     /translation="MSEKDVIKETRPRMETVIEDFRRKLASIRTGRAAVSLLDTVMVD
                     YYGTMTPLNQMASVHAPEPQMLTVQPWDQTQIGAVEKAIRAADLGLNPSNDGKLVRVP
                     IPALTEERRKQLAKQVHDIAEDHRTAVRNVRRDANEKLKKMLKDKLISEDAERDALEE
                     IQKLTNNYIGKIDELSKTKEHEIISV"
     gene            3221..4183
                     /locus_tag="VFO72_11390"
     CDS             3221..4183
                     /locus_tag="VFO72_11390"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9479943.1"
                     /translation="MPTAQTIRDEQTGQVFILVPEGKADIFAAWISAGYSIASIAFLA
                     WLLLDTWSGQYTALKKILSPEILATVTNSTVFRLVVYTAIGGGMGASINNLRSFIQWH
                     AERQAFGWRFIWKYIAMPPLGAALAALVYGILQGGMAVFNGGTAIGASGGNSITSLSA
                     WATGTLAGYGAHKVFIWLDEKVNSLFRVEQTKVNVPDVSGKSPEEAREILINTQLEMG
                     ESTTATTTQPQNFGKVIGQTPPAGTELACKSKVTLVIGAPAGAEGADTTRTDGAAKTE
                     EEAKQVAQNGKLGPTSEESKVGADSGSQGVTGGSQQTKTTQEGG"
     gene            complement(4243..5424)
                     /locus_tag="VFO72_11395"
     CDS             complement(4243..5424)
                     /locus_tag="VFO72_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479944.1"
                     /translation="MRTFLLAIALALLTQGADPGIAAKHAIGEVTTIDAAAKQITIKT
                     DAGSTVTVSVSDKTTYKRLAPGEQSLTNATDIAFTDVTEGDRIMARGTVAEDKKSVPA
                     LQVIVMTKGDLAKKQEAERTEWRRRGILGVITALKPDTKEITISNRTMAGMQSVVIPV
                     SDKTEMRRYAPDSIKFSDAKPSTFDELKIGDQLRAVGDRTEDPLRFNAQKIVTGSFRT
                     VGGVVTAVDPATGEIKINELEKKTPLTIVVKQDAVLRRFPSDIGAMMGAFGRGGQGGP
                     GSARAGQGSQPQGGQAQPARPQGGGAPGAGGPGGRGGINDMLERLPIISLADVKVGDT
                     IIVSSTQGVDPARLTAISLVTGADTLLAMLAPRPEPGQATPNPAAGLGNSGITFGIGL
                     P"
     gene            complement(5438..>6275)
                     /locus_tag="VFO72_11400"
     CDS             complement(5438..>6275)
                     /locus_tag="VFO72_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479945.1"
                     /translation="DANTDAVVVVNGVSDSTVFGVGNSVRITGTVKQGAMALGGDVIV
                     EGVVEGDVATIGGSVIQLPGSSIGGDVIVLGGTYRADDISARRNPKSMTIMYAGYQQE
                     LRDMMRHPTGLFSPRWTPTYLGTRLLVVLFWFVVALAFTSAMPGTISRGVARLQLTSV
                     RVAAIGLIGVVVLFVSVLVCLWIMPEPVQVLVGLLALLLWLVASVFGRVILYAATGKW
                     LQRKYVPFGRNSEAVALLLGTSFWVLLTSLPYVWPFMAAFILIISFGLALTARYRKIV
                     TV"
BASE COUNT         1506 a         1749 c         1806 g         1214 t
ORIGIN      
        1 gacaagtcga gcggctgcgg cgagtacatt aaaccaattg acgagaagga gactttagtt
       61 tggtttcggt aaccatgaaa gagctgctgg aggccggtgt tcactttggc caccaggtac
      121 ggcgctggaa cccaaagatg aaggaataca tctttggcga gcgcaatggc atttacatca
      181 tcgacctgca gaagacgcag cgcatgttcc gcgaagcgat cgccttcgta accaacctga
      241 tcgctgaaga caaaggcaga acagttctct ttgtcggcac caagcggcaa gcacaggacg
      301 cgatccgcga agaagcggaa aagtgcggtc agtatttcgt gaatcagcgc tggcttggcg
      361 gcttgctgac taacttccag accgtccaaa agtcgatcaa acggcttaag gatctcgaga
      421 gcatgcagac cgacggtcgt tacgagaaac taaccaaaaa agaacgcatc aaactcgacc
      481 gcgaacgcga gagtctgaac aagaacctct caggtatcaa gatgatgtcg cggctgcctg
      541 acgctgtttt catcatcgac gtcaagaagg aagagatcgc cgtcgccgaa gccaaccggc
      601 tcggcattcc gatcgtcgca gtcgtcgata caaactgctc tcccgaaggc attgactatg
      661 tgattcccgg aaacgacgat gccttgcgtg ccgttcggtt gtttgcttct cgcatcgccg
      721 acgccatcgt tgaaggcaat cagattgcta cagaaggcgg cgtagtcacc actgaaccaa
      781 tcgaagaacc ggaagaaggt gcgggagaag ctgccgcggc cgagttcgcg cccactggcg
      841 cagaggccgt caacggggag gaggcgcaat ccgaatccac gactacagcc ggcgctaccg
      901 aaacggacgt agcgcgcgac gagagccctg aatcagtcgt ggcggcgagc taagcgatat
      961 tcgtgacttt aggtgagcgc agccttcaag cggacatccg ccgggctgcg tttttcattg
     1021 aagacaaatc caagcatgcg gagaaattgg taatcatggc tgagataaca gcagcggcag
     1081 tcaaacagct tcgggagaag accggagcgg gaatgatgga gtgcaagaac gcactcgttg
     1141 aagcaggcgg caacgaagac aaagcaatcg acattctgcg cgaacgtggt ttggcatcgg
     1201 cgaagaaacg cgaaggccgc atcgccgcgg aaggaattgt tggttcttac attcacatgg
     1261 gtggcaaggt tggtgtgctg gtcgagttga attgcgaaac ggacttcgta gcgcgtggcg
     1321 aagagtttca gcagttggtc aaagacatcg cgatgcacat cgcggccgcg gaaccgcgtt
     1381 acgtttcgcg tgaggaagtc cctgaaagtg tgctcgagaa agaacgcgag atcgcgcgcg
     1441 cgcaagccaa aaacgatcca aagatggcga ataagcccga gcaggtgatc gagaagatcg
     1501 tagaaggacg gctgaacaag ttctacgagg agacggtgtt gagcgatcag ccattcgtga
     1561 aagatccgtc gaagactgtc gctgaattag tgacggaaaa ggttgcaaag accggtgaac
     1621 gcatcgcgat tcgccggttc actcgttaca agatgggtga aggactcgag cgccgtgcag
     1681 atgatttcgg tggcgaagta gcgtcaatgc tgcgatgaag aacgaggctt ccaatttggc
     1741 ctatcgccgc gtgctgttga agatctccgg tgaggcgctg atgggcgagc agaattacgg
     1801 catcgacgtt gacgtcgctc gcagcgtcgc cgaggagttg aaagccgtgc atcaacttgg
     1861 cgtgcaggtc gctgttgtcg ttggcggcgg aaacatcttt cgcggtgtgt cgaagagtgc
     1921 cggcaacatg gaccgttcgt cggcagatta catcggcatg cttgcgaccg tcatgaacgc
     1981 tgtcgtgttg caagatgcgc tcgagaagat tgaagtcgat acgcgcgtgc tttcagcggt
     2041 cgacatcccg caactggcgg agccgttcat tcggcgccgt gctgttcgtc atctcgaaaa
     2101 gggacgcgtg gtgatctttg ctgccggcac gggcaatccg tatttcacca ctgactcggc
     2161 ggccgcactg cgagcgctgg agattagagc ggacatcatt ctcaaagcga ccaaagttga
     2221 cggcgtttat tcggccgacc cgatcacaga cgccacggcc gaactcttca acgagatcag
     2281 ttatcgtcag gtgctcgaac gagagctgaa agtgatggac gcttcggcca tctccctgtg
     2341 catggacaac aatctgccaa ttatcgtctt taatatgcgc cagaggggaa acatcaaacg
     2401 cgtggtgatg ggcgaaagcg gagtcgggac gaaagtcggg taggacagac attcatgtct
     2461 gtctcttcga caggcaagaa tgcctgtcct acggagtgca gcgagatgag tgaaaaagac
     2521 gtaatcaaag agactcgacc gcgaatggaa acagtcatcg aagacttccg gcgcaagctt
     2581 gcgagtattc gcacgggtcg cgctgcggta agtctgttgg atacggtgat ggttgattac
     2641 tacggcacga tgacgccgtt aaatcagatg gcttcggtgc acgcgccgga accgcagatg
     2701 ttgacggttc aaccgtggga ccaaactcag atcggcgcgg ttgagaaagc gatccgcgcc
     2761 gccgacctcg gtctcaaccc atccaacgac ggcaaactcg tgcgcgttcc gattccagcg
     2821 ttgacggaag agcgacgcaa acaacttgcg aagcaggtcc acgacatcgc cgaagatcat
     2881 cgcactgctg ttcgcaacgt acggcgcgac gcaaacgaga aactcaagaa gatgttgaaa
     2941 gacaagctca tctccgagga cgccgagcgg gatgctcttg aagagatcca gaaactcacc
     3001 aacaactaca tcggcaaaat cgacgaactc tccaagacca aagagcacga gatcatcagc
     3061 gtgtagaacc acggatcgca cagatccgtg tcgtcagtgt tcaaattaat ccggcaatgc
     3121 ggctttgcgc gaaaaggcgt tcaagttgct cgagtcgctc gcggaccagc catgaaacgt
     3181 ttgatcccat cgtgcacata tagaatagga ggtcatgatt atgccaaccg ctcaaacaat
     3241 tagggacgaa cagactgggc aggtcttcat cctggttccg gaaggaaagg cggacatatt
     3301 cgccgcgtgg atcagcgccg gctactcaat cgcttccatc gcgtttctcg cgtggctgtt
     3361 actcgatacc tggtccggtc aatacacggc cctgaagaaa atactgtcac ctgaaatcct
     3421 ggccactgtt acaaacagta ccgtcttcag gctcgtggtc tacaccgcga tcggcggcgg
     3481 catgggcgcc tcaatcaaca accttcgcag cttcatccag tggcacgccg aacgacaggc
     3541 atttggttgg cgttttatct ggaagtacat cgctatgccg ccgctgggcg cggcgctcgc
     3601 agcgctggtt tacggcatcc tccagggagg catggctgtt ttcaatggcg gcacggctat
     3661 cggcgccagc ggcggcaact cgattacctc gctttcagcc tgggccaccg ggaccttagc
     3721 tggttacggt gcacacaagg tgttcatctg gttggatgag aaggtgaact cgctgttcag
     3781 ggtggagcag acaaaggtta atgttcctga tgtttcaggt aaatcccccg aagaagcccg
     3841 agagattcta atcaacactc aactcgagat gggcgagagc actaccgcaa caacgaccca
     3901 gccacaaaat tttggaaagg tgatcgggca aactccacca gcgggaaccg agctcgcttg
     3961 caagagtaaa gtgactctcg tcattggcgc gcctgccggc gctgaaggag cagatacaac
     4021 gcgaacagac ggagccgcca agacggaaga ggaggcgaag caggtcgcgc aaaacgggaa
     4081 gcttggtcca acgagcgagg agtcgaaagt cggagccgac agtggatcgc agggcgtcac
     4141 cggcggcagt caacagacca aaacaactca agagggcgga taagaactat cgcccgcgct
     4201 gtgttctgcc ctgcgatcaa gcagcgttat tgccgccgac ggctatggca agccgatgcc
     4261 gaaagtgatg ccgctattgc ctaaacccgc ggccgggttc ggcgtcgcct gaccgggctc
     4321 ggggcgcgga gcaagcatcg cgagcagagt gtcggcgccg gtcaccaacg aaattgccgt
     4381 tagacgcgcc ggatcgaccc cctgcgtact ggaaacgatg atggtatcgc caaccttaac
     4441 atccgccaaa gaaataatcg gcaatctctc cagcatgtcg ttgataccgc cacgaccacc
     4501 aggtcccccg gcgcccggcg caccaccgcc ttgcggtctc gcgggctgtg cctggccgcc
     4561 ctgcggttga gatccctggc cggctcgagc gctgccgggt ccaccttgtc caccacggcc
     4621 gaacgcgccc atcatggcgc cgatgtctga aggaaagcgg cgcaacactg catcctgttt
     4681 gacaacgatc gtcagcggcg ttttcttctc gagttcgttg atcttgatct cgccggtcgc
     4741 cgggtcgacg gcagtgacaa cgccgccaac ggtacggaaa cttccggtta cgatcttctg
     4801 tgcgttgaaa cgaagcgggt cttctgtgcg atcgccaacg gcgcgcagct gatcgcctat
     4861 tttgagttcg tcaaacgtgc tgggcttcgc atcgctgaac ttgatcgagt cgggcgcgta
     4921 gcggcgcatt tcagttttgt cgctgaccgg aatcactacc gattgcatac ccgccatcgt
     4981 gcgattggag atcgtgatct ctttcgtgtc tggtttgagt gcggtgatca cgccgagaat
     5041 gccacgtctg cgccactccg ttcgttctgc ttcctgcttc ttcgccagat cgcctttggt
     5101 catgacaatg acctgcaaag ccggcaccga ctttttatct tcggcaacag ttccgcgcgc
     5161 catgatgcga tcgccttcag ttacgtccgt gaaagcgatg tcggttgcat tcgtcagcga
     5221 ctgctcgccg ggagcgagac gtttgtacgt cgtcttgtcg ctgactgaaa cagtaaccgt
     5281 cgaaccggcg tctgttttga tcgtaatttg cttcgcggca gcatcgatcg ttgtgacttc
     5341 accaatcgca tgcttcgcgg cgatgcccgg atcggcgcct tgcgtaagca gtgccagcgc
     5401 aattgccagt aagaacgtcc tcatctcctg tcctttccta cacagtaacg attttgcggt
     5461 atcgagcggt caaagcgaga ccgaaactga tgatcaaaat gaaagccgcc atgaacggcc
     5521 aaacataagg cagcgaagtc agcaacaccc agaaactggt ccctaacaag agtgctactg
     5581 cttccgagtt tcggccgaaa ggcacgtact tccgttgcag ccattttccg gtcgccgcat
     5641 acaaaataac cctaccgaac acgctcgcaa ccagccaaag caacagtgcc agcaaaccca
     5701 cgagcacctg caccggctcg ggcatgatcc acaaacagac cagaacgctc acaaagagca
     5761 ctaccacgcc aatcaacccg atagccgcaa cacgcacgct agttaactgc aaccgcgcca
     5821 cgccgcgact gatcgtgccg ggcatcgccg acgtgaatgc cagcgcgacc acgaaccaga
     5881 acagcacgac gagaagtctg gtccccaaat acgtcggagt ccagcgcggg ctaaacaatc
     5941 cggttggatg gcgcatcatg tcgcggagtt cctgctggta acccgcgtac atgatcgtca
     6001 tgctcttcgg attccgcctg gccgaaatgt cgtcggcgcg gtaagtgccg ccaaggacga
     6061 tgacgtcgcc gccgatgctc gaacccggca gttgaattac cgaaccgcca atcgtcgcga
     6121 cgtcaccttc aacgacgcct tcgacgatca cgtcgccgcc gagcgccatc gcgccctgtt
     6181 ttacggtgcc ggtgatccgg acggagttgc ccaccccaaa gacggtgcta tcgctgacac
     6241 cattaacaac gaccacggcg tcagtgtttg cgtcc
//
LOCUS       DASTEY010000654         6170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_377547, whole genome shotgun sequence.
ACCESSION   DASTEY010000654 DASTEY010000000
VERSION     DASTEY010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377547"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFO72_11405"
     CDS             <1..848
                     /locus_tag="VFO72_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET9479946.1"
                     /translation="ANAGVPTLLLDIAPHELTPEEQAKGLTLQSPQVRNRIARNGLEA
                     AKNAKPAAFFTPDHASLVTIGNFDDDLAKLKDRDLIIEAVVENLEIKRSLYERVEQHC
                     RPGSIVASNTSGIPIHLLAEGRSDDFKQHFLGVHFFNPPRYLHLVEIIRTEWTKPEVS
                     CFLYGFLDERLGKGVVPAKDRPNFIANRIGTFGALYTIKTMLDDGYSIEEVDKMTGPV
                     VGRPKSATFRTFDLVGLDVFTHVIKNLYEALPEDEEREMFVVPDVLAQMVQRGLLGNK
                     TKGGF"
     gene            951..2345
                     /locus_tag="VFO72_11410"
     CDS             951..2345
                     /locus_tag="VFO72_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9479947.1"
                     /translation="MAKNIEDLPERIKTLVWNKDRVGAFLWKTLAKTLTYTAKRIPEI
                     ADNVVEVDRAMRWGFGWELGPFEVWDAIGVGKSVARMKEDGLEVPANVEQMLAAGATS
                     FYKTENGQQSYFDFNQGKYVPLADPPGVLILKSIKDRTGVIKKNAGASLIDIGDGVAC
                     LEFHSKMNAIGGDTLQMLKFALGEVDKNFVGFVVGNQGPNFCVGANIMLMLMEAQEEN
                     WDELDMMARVFQQSTMSLRYSPKPVVVAPFQMVFGGGCEMVLHADRVRAAAETYIGLV
                     EVGVGIIPAGGGTKELLLRTLDLIPKDTSDADPFPFVKRAFETIALAKVATSAEEARA
                     LGFLAPDDTVSMNQDRLIADAKKEVLALAASGYVAPQQRTDILALGEPALATFKLGIH
                     QMKRAGYISDHDAEIGTQLARVLTGGDLNHPTRVSEQYFLDLEREAFLKLVSMRKTQE
                     RLAHMLKTGKPLRN"
     gene            2404..3585
                     /locus_tag="VFO72_11415"
     CDS             2404..3585
                     /locus_tag="VFO72_11415"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9479948.1"
                     /translation="MRDAVIVSAVRTAVGKAPKGTLRTTRPDEMGAAVIKEAVGRVEG
                     LGTAEIEDVIMGCAMPEAEQGMNVARAAAIRAGLPVETSAMTINRFCSSGLQSIAMAA
                     DRIKTGAAEVIVAGGLETMSMIPMGGHIIRPNPYLVDHYPDFYLSMGLATENVARKYE
                     VTREEQDEFALRSHTRAAAALDAGKFKDETVPLHVVLEELDEKGKKQRREVVFDKDEG
                     VRRDTSAEGLAKLKPAFQVKGTITAGNASQMSDGAAAAVVMSDSRARALGAKPLARFV
                     AYATAGCPPEEMGIGPVFAIPKVLKLAGLTLNDIDLIELNEAFAAQSLAVIKTLGLDP
                     DKVNVNGGAIALGHPLGCTGAKLTASVLSELERRNGRYGMVTMCVGGGMGAAGILERL
                     D"
     gene            3651..5435
                     /locus_tag="VFO72_11420"
     CDS             3651..5435
                     /locus_tag="VFO72_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9479949.1"
                     /translation="MSAVAEQKKLFKGGSFLIEERNPDEIFTPEDFTEEHRMIADTTR
                     QFVDNEVTPHIDQLEKHDWKLARELVRHAADLGLIGANIPEEYGGLGLDQTSGALIGE
                     NIGRSASFATTLGAESGIGLLPIVYFATDAAKQKYLPKIATGELITAYALTEAGAGSD
                     AMAGKATARLSDDGKEYILNGEKMWITNGGFADIFIVFAKVDGDKFSAFIVERQPGLT
                     SGAEEHKMGIKGSSTTALVLSDVRTPVENLLGEVGKGHKIAFNVLNIGRFKLGAMCAG
                     GMKLMVQESVRYANERHQFGKPISSFGAIKSKLAEQAIRTWVGESMIYRTLGMIEAGI
                     GAVDSKDMDARLRAIEEYAAECSVIKVALSEYCDYVADEMVQIYGGYGYSADYPAERA
                     YRDSRINRIFEGTNEINRMLIPGRLMKSALSGNLALLPAAQALMDEVLSPQLPSLDDD
                     DALLAVEEKLTTNAKKVALMTLGTAAQKYMMKLADQQEILMGIADIIMDTYAMETAIL
                     RARKLAASQGETAAARYLDMTRVFCNDAVGRIEARAKNTLAGMSEGDELRTLLAALRR
                     FTKQQPLNTIAARQRIADDLIAANKYIY"
     gene            5566..>6170
                     /locus_tag="VFO72_11425"
     CDS             5566..>6170
                     /locus_tag="VFO72_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9479950.1"
                     /translation="MRVIPISVPTPFYVGPVNVYLIAEEPVTIIDTGPKTKEAIAALR
                     AGLRAAGFLLSDIRRIVLTHAHEDHCGLARAVRDEAKNAEVLVHDWETGHRHSRLQYD
                     EHRQLLVRAGVPEHEIAGMRRLYEGVRQYADSLDDADCGPLWDEAELEFESGSLRILH
                     TPGHTPGSCSFLREADRTLIAGDCVLKRITPNPILSPDPLNP"
BASE COUNT         1397 a         1652 c         1866 g         1255 t
ORIGIN      
        1 tcgctaacgc cggtgtgcct acgctcctcc tggatatcgc gccacatgag ctgacacccg
       61 aagaacaagc caaaggactg acgctccaaa gtccgcaagt gcggaaccgc atcgctcgca
      121 acggcctcga agccgcgaag aacgcaaaac ccgccgcctt cttcacgccc gaccatgctt
      181 cgctcgtcac catcggtaac ttcgatgacg acctcgcgaa actcaaagat cgcgacctga
      241 tcatcgaagc cgtcgtcgag aatctcgaaa tcaaacgttc gctctatgag cgcgtcgaac
      301 agcattgccg gccgggctca attgtcgctt caaacacgag cggtattccg attcacctcc
      361 tcgccgaggg acgttcagac gacttcaaac agcatttcct cggcgtccat ttcttcaacc
      421 cgccgcgtta tctccatctc gtcgaaataa ttcgcacgga atggaccaag cccgaagtct
      481 cctgttttct ctacggcttc ctggacgagc ggttgggcaa aggcgtcgtg cccgcaaaag
      541 atcggccaaa cttcatcgcc aatcgcatcg gcactttcgg cgcgctctac accatcaaga
      601 cgatgctcga cgacggttac tccatcgaag aggtcgacaa gatgaccggt ccggttgtcg
      661 gacgcccgaa gtcagccacc ttccgaacgt ttgatttagt tggtctggac gtctttacgc
      721 acgtcatcaa gaatctttac gaagcgctgc ccgaagacga agagcgcgag atgtttgtcg
      781 ttcccgacgt gctcgcgcag atggtccaac gcggcctgct cggtaacaag accaaaggcg
      841 gtttctaacg caagcagaaa ggcgagggcg aaaagcgcga gatctggacg ctcgataccg
      901 caactctcga ttaccgcccg tctgaaaaag tcaaacttcc cgcgctcgac atggcgaaga
      961 acatcgaaga cttgcccgag cgaattaaga ctctcgtttg gaacaaggac cgcgtgggcg
     1021 catttctttg gaagacgctg gcgaagacgc tcacctacac ggcgaaacgc attcccgaga
     1081 tcgccgacaa cgtagtggaa gtcgatcggg cgatgcgctg gggcttcggt tgggagttgg
     1141 gcccattcga agtttgggac gcgatcggag tcgggaagtc agttgcgcga atgaaggaag
     1201 acgggctcga ggttccagcg aatgtggaac agatgctggc cgcgggtgct acttcgttct
     1261 acaaaacaga gaacgggcaa cagtcgtatt tcgacttcaa ccagggcaag tacgttccgt
     1321 tggcggaccc gccgggagtt ctgattctca agtcaattaa agatcgcact ggcgtaatta
     1381 agaagaacgc cggcgcatcg ttgatcgata tcggcgatgg tgttgcgtgt ctcgagttcc
     1441 attcgaaaat gaacgcgatc ggtggcgaca ccttgcagat gctgaagttt gcgctcggcg
     1501 aagttgacaa gaactttgtc ggtttcgtcg tcggaaacca gggaccaaac ttctgcgtcg
     1561 gcgccaacat catgctgatg ctgatggagg cgcaggaaga gaactgggac gaactcgata
     1621 tgatggcgcg agtttttcag cagtcgacga tgagtttacg ctactcgccg aagcccgtgg
     1681 tcgtcgcgcc gtttcagatg gtgtttggtg gcggttgtga gatggttttg cacgcggatc
     1741 gcgtgcgagc ggcggcggaa acgtatatcg gattggtcga ggttggcgtg ggaattattc
     1801 ccgcgggtgg cggcactaag gagttgctgt tgcgcacgct cgatctgatt ccgaaagata
     1861 cgagcgacgc ggatccgttt ccgtttgtga agcgtgcttt cgaaacgatc gcgctggcga
     1921 aggtcgcgac ctctgctgaa gaggcgagag cccttggctt cctcgcacct gacgatacgg
     1981 tttcgatgaa tcaggatcgt ttgattgccg acgctaagaa agaggtgttg gcgctcgccg
     2041 cgagtggtta cgtagcgccg cagcagagaa cagacattct ggcgctgggc gaaccggcgc
     2101 tggcaacttt caaactcggc attcatcaga tgaagcgcgc tggatacatt tcggatcatg
     2161 atgctgagat cggtacgcag ttggcgcgag ttttgacagg tggagacttg aatcacccga
     2221 cgcgcgtttc cgaacagtat tttttggatc tggagcgcga agcgtttttg aagttggtca
     2281 gcatgcgcaa gacgcaggaa cggctcgcgc atatgttgaa gacgggcaag ccgctgagaa
     2341 actaggccac ggactcacgc ggacatacac ggatgagtca gtaagttaaa agtagggttg
     2401 actatgagag acgcagtaat cgtatcagca gttcgcaccg cggttggcaa agctccgaag
     2461 ggcacgttgc gcacgacgcg gcctgacgag atgggcgcag ccgtgatcaa ggaagcagtt
     2521 ggtcgcgttg aggggctcgg cactgcggaa atcgaagatg tgatcatggg ttgtgcgatg
     2581 cctgaagcag agcagggaat gaacgtcgca cgtgcggcag cgattcgtgc cggactgccg
     2641 gttgagacga gtgcgatgac gatcaaccgg ttctgttcgt cgggtttgca gtcgatcgcg
     2701 atggccgcgg accggatcaa gacgggcgcc gcggaagtga tcgtcgccgg tgggttggaa
     2761 acaatgtcga tgatccctat gggcggacac atcatccggc ccaacccgta tctcgtcgat
     2821 cactatccgg atttctactt gagcatggga ctggcgactg agaacgttgc gcgcaagtat
     2881 gaagtgacgc gcgaagagca ggacgagttt gcgttgcgct cgcacacacg cgccgcggct
     2941 gctttggatg ccggcaagtt caaagacgag acggttcctt tgcacgtcgt gctggaagag
     3001 ttggatgaaa agggaaagaa gcagcggcgt gaagttgtgt ttgataaaga tgaaggcgtg
     3061 cgacgtgaca cgtcggctga ggggttggct aagttgaaac cggcttttca agtgaaaggc
     3121 acgatcactg cgggcaatgc gtcgcagatg tcagacggcg cggcggcggc ggtggtgatg
     3181 tcggattcgc gtgcgcgcgc gttgggcgcg aagcctttag cccgtttcgt ggcttatgcg
     3241 actgcgggtt gtccgccgga agagatggga attgggccag tgttcgcgat tccgaaagtt
     3301 ttgaagctcg ccggacttac actcaacgac atcgacctca tcgaactcaa cgaggcgttc
     3361 gcggcgcagt cgctcgcggt aataaaaacc cttggcctgg acccggacaa agttaacgtc
     3421 aacggcggcg cgatcgcgct gggccatccg cttggctgca ccggagcaaa gctcactgcg
     3481 agtgtcctga gcgagttgga gcgccgcaac gggcgatacg gaatggtcac gatgtgtgtc
     3541 ggcggtggca tgggcgccgc cggaattctc gaacgacttg attgagccac gtgtcacaca
     3601 gacgtataaa cgaagcagta taagtcggtg gctttaaagg agaacaaata atgtcagccg
     3661 tagccgaaca aaagaaactt ttcaaaggcg ggtcgttcct gattgaagag cgaaacccgg
     3721 atgagatctt tactcctgag gatttcacgg aagagcatcg catgatcgcc gacaccacgc
     3781 ggcaattcgt cgataacgaa gtgactccgc atatcgacca actcgagaag cacgactgga
     3841 agctcgcgcg tgaactcgtc cgccacgcgg ccgacctcgg actgatcggc gcgaacatac
     3901 ccgaagagta tggtggcctg ggtctcgatc aaaccagcgg cgctctgatc ggtgaaaaca
     3961 tcggacgctc cgcgtcattc gctactacac tcggcgccga aagcggcatc ggtcttttgc
     4021 cgatcgttta cttcgccact gacgccgcca agcagaaata cctgccaaag atcgccaccg
     4081 gcgaactgat cacggcttac gccttgaccg aagcgggtgc cggttcggac gcaatggccg
     4141 gcaaggccac cgcgcgtctc agcgacgacg gcaaagaata cattttgaac ggcgagaaga
     4201 tgtggattac caacggcggc tttgccgaca tctttatcgt gtttgcaaaa gttgatggcg
     4261 acaagttctc ggcgttcatt gtcgaacgcc aaccgggtct gacgtccggc gccgaagaac
     4321 acaagatggg catcaagggt tcgagcacga ctgcgctcgt gctttccgac gtgcgcacgc
     4381 cggttgagaa cctgctcggt gaagttggca aaggacacaa gatcgctttc aacgtgctga
     4441 acatcggccg cttcaaattg ggtgcgatgt gtgccggcgg catgaagcta atggtccagg
     4501 agtcggtgcg ttatgcaaac gaacggcacc agttcgggaa accgatctct tcgttcggcg
     4561 caattaagag taagctggcg gaacaggcga ttcgcacgtg ggttggcgag tcgatgatct
     4621 atcgcacgct cggcatgatc gaagccggca tcggcgccgt cgactcgaaa gacatggacg
     4681 cgcggctgcg agctattgag gaatacgcgg ccgagtgttc ggtcattaag gtcgcgctct
     4741 cggagtactg cgattacgtc gccgatgaga tggtccagat ctatggcggc tatggttact
     4801 ccgccgacta tccggccgag cgcgcttatc gcgactcgcg tatcaatcgc atcttcgaag
     4861 gcacgaatga gatcaaccgc atgttgatcc cgggccgttt gatgaagagc gcgctgagcg
     4921 gcaacctggc gttgcttccg gcggcgcagg cgttaatgga tgaagtgctg tcgccgcaac
     4981 tgcccagtct tgatgacgac gacgcgctgc ttgcggtcga agagaagttg actactaacg
     5041 ccaagaaggt cgcgctgatg acgctcggca ctgccgcgca aaagtacatg atgaaactcg
     5101 ccgaccagca ggaaatactg atgggcatcg ccgacatcat catggacacc tacgcgatgg
     5161 agaccgcgat cctgcgcgct cgcaaactgg cagcttcgca gggtgaaacg gctgcggcgc
     5221 gttatctcga catgacgcgc gtcttctgca acgacgccgt cggccggatc gaagcccgag
     5281 cgaagaacac actcgcaggt atgtctgaag gagatgaact tcgtacgctg ctcgccgcgc
     5341 tacgccgctt tacgaagcag cagccgttga acacgatcgc tgcccgccag cgtattgcag
     5401 acgatctaat tgcggccaac aagtacattt attgattgcc gatttttgat tttcgattgc
     5461 cgattgccga tttgtcgcgg gtgataggta ccgtcccggt cgataagtaa acaaaattgg
     5521 caattggcaa ctgaaaatcg gcaatcggca atcggcaatt ccttgatgcg cgtcatccct
     5581 atctcagtgc caacgccttt ctacgtcggt cccgtaaacg tctatctaat cgctgaggag
     5641 ccggtgacga tcatcgacac cggccccaag acgaaagaag cgatcgcggc gttgcgtgcg
     5701 ggtctgcgcg cggccggttt tctgctttcc gacatacgac gaatcgtact cactcacgct
     5761 catgaagatc actgcggact cgctcgcgct gtgcgcgatg aagcgaagaa cgcggaagta
     5821 ctcgttcatg attgggagac ggggcaccgt cacagccggc tgcaatacga cgagcatcgc
     5881 caactactcg tgcgtgcggg tgttcccgaa catgagattg caggaatgcg ccggttatac
     5941 gaaggcgtca gacaatatgc tgattcgctg gacgatgccg actgtggtcc gctgtgggac
     6001 gaggctgaac tcgaatttga atccggcagt ctgcgcattc tgcacacgcc gggtcacacg
     6061 cccggctcgt gttcgtttct tcgggaagcg gatcgcacgc tgatcgctgg cgactgcgta
     6121 ttgaagcgaa tcacacccaa tccgatactc tcgcccgacc cgttgaatcc
//
LOCUS       DASTEY010000655         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_855593, whole genome shotgun sequence.
ACCESSION   DASTEY010000655 DASTEY010000000
VERSION     DASTEY010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855593"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..722)
                     /locus_tag="VFO72_11430"
     CDS             complement(324..722)
                     /locus_tag="VFO72_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9479951.1"
                     /translation="MDDSVSAILYPTVLLAEDSQDTRKMLKRAFELKGYRVFEAEDGQ
                     EALDIARRYRPNLIVIDLNMPVLDGLETVKNLREREGPGEEIPIVAITAYDVPGMEEA
                     ALETGCNKYLSKPLDLAELDRTLKNLGFII"
     gene            818..2107
                     /gene="aroA"
                     /locus_tag="VFO72_11435"
     CDS             818..2107
                     /gene="aroA"
                     /locus_tag="VFO72_11435"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9479952.1"
                     /translation="MKLVPAQRLSGRLRLPGDKSISHRAVLIAALAGGPSEISNFSTA
                     RDCASTLECLRQLGVPIEQHGDKLDFPGNQKLLAPGKPLDCGNSGSTMRILAGVLAGH
                     DLTAELTGDESLSSRPMRRIIEPLTLMGAQIEAQDGKPPLKIRGRSRPDAITYKLPVA
                     SAQVKSAILFAALNAEGRTTVIETVPSRDHTERLFNGFGVPVITNSDLSVALDGPARF
                     NGGAITIPGDISSAAFFIAAAMLLPGSELVIEGVGLNPTRAAFLSVLKSWGADISQTD
                     LRTERNEPVGTVKVRGGIHGTASASDRMLSGNLIPSLIDELPLLAVVGTQIQGEIEIR
                     DASELRLKESDRLATTAHNLRAMGADVEELPDGLRISGPTQLHGAVIDSYGDHRIAMA
                     FSVAALLASSNTEIRGSESAGVSFPEFFGLLDSLTER"
     gene            2104..2649
                     /locus_tag="VFO72_11440"
     CDS             2104..2649
                     /locus_tag="VFO72_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET9479953.1"
                     /translation="MKPVAPIIITGFMGCGKTETARALANRRGLALTDLDRAIFNQTG
                     RTAAQLITQEGEQAFRAIETKMLRELLLNRGTGVIALGGGAWITDENRELISEHNGLS
                     VWLDVPFELCWQRIATSAEVRPLGRTREDAEKLYLLRRPIYQLATIHLPVRADEPLEN
                     IVARLEAALASYHRDRNERSD"
     gene            complement(2616..3185)
                     /locus_tag="VFO72_11445"
     CDS             complement(2616..3185)
                     /locus_tag="VFO72_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479954.1"
                     /translation="MNFDTDRIPQLDEVSRKVEAISGFRLEPVAGLVEPRIFLESLAR
                     GVFLCTQYIRHHSTPLYTPEPDVAHEIIGHAVTLASKRFAEINRMFGEAVRRTASSSE
                     LDRLARVYWFTIEFGVLRENGEIKAYGTGLLSSAGELAEMHKAELRPLDLEAAANHVY
                     DPTHYQAILFCAESFDKMDQSLRSFLSRW"
BASE COUNT          733 a          973 c          888 g          684 t
ORIGIN      
        1 gggtaaactt aactcggtcg tctctacggt caacccttat gcaaggcctc tccgaaagcc
       61 actgcgcgag gactctggct gctttctgta ccttcagaca ctgatcagga atccgagggt
      121 ttaacacgct ggatgttcac ctgtcaagaa acgctcgccc cgacgccgaa gacgcgcgca
      181 cgtagcgccg aaggcgcgcg ctcggagcgc cgaagatgcg cccaggtaag cgccgaaggc
      241 gcggccttcg gcgctgttct gttgccccgt ggctaccgta tcgttgcgcc tacgggtgct
      301 agtgatcaaa aactgaagac gcctcagatg atgaaaccga ggttcttgag cgtacggtcg
      361 agttcggcta gatcgagtgg tttggaaagg tatttgttac aaccggtttc aagagcggcc
      421 tcttccattc cgggaacatc gtaggccgtg atggcaacaa tcgggatctc ctcgcccggt
      481 ccttcgcgtt cgcgcaagtt tttgacggtt tccaaaccgt cgagcaccgg catattcagg
      541 tcgatcacga tcaaattcgg cctgtaacgc cgcgctatat ccagcgcttc ctgcccgtct
      601 tccgcttcga agacacgata ccccttcagc tcgaacgctc gtttgagcat ttttcgagtg
      661 tcctgggaat cttccgcaag caaaacagtc gggtacagta tcgcagacac ggagtcgtcc
      721 atcgggctaa tgccctgagt actgatatga ttcctctttc cggggggaac cgccagcagg
      781 gggtaactct aagcgggttg tgccctattt ataacacatg aaactagttc ccgcacaacg
      841 actgagcggc agactgcgac tgccggggga caaatctatt tcacaccgcg ccgtactgat
      901 cgcggcgctc gccggcggtc catcggaaat ttcaaacttt tccacagccc gcgactgcgc
      961 ttcgacactc gagtgtctgc gtcaactcgg tgttccgatt gagcagcacg gcgataagct
     1021 tgactttcca ggtaatcaaa aacttctcgc accagggaag ccgttggact gtggcaacag
     1081 cggctcgacc atgcgaatac tcgccggcgt gctggcgggc cacgacttga ccgccgaact
     1141 aaccggcgat gagtctttga gctcgcgccc aatgcgccgc atcatcgagc ctttgaccct
     1201 gatgggagcg cagatcgaag cacaggatgg caaaccgcca ctgaagatcc gcgggcgtag
     1261 cagaccagac gcaattacgt acaaacttcc ggtcgccagc gcacaagtaa agtcggccat
     1321 tctgtttgca gcactgaacg ctgaaggccg taccacagtt atagaaacgg ttccgtcacg
     1381 cgaccacacg gaacgtctct tcaacgggtt cggcgtaccg gtaatcacaa acagcgacct
     1441 gtcagtggcg cttgatggtc cagcacgttt caacggtggc gcgatcacga ttccgggcga
     1501 catctcttcc gcagcttttt tcattgccgc cgcaatgctc ctgccgggct cggaactcgt
     1561 gatcgaagga gtcgggttga atccaacgcg cgctgcgttc ttgtcggtgc tgaaatcgtg
     1621 gggcgccgat atttcgcaaa ctgatttacg gacggaacgc aacgagccgg ttggaaccgt
     1681 caaagtacgc ggcggcattc atggcaccgc cagtgccagt gatcgaatgc tgagcgggaa
     1741 tctaattccg tcgctgatcg acgagttacc gttgcttgcg gttgttggaa cacagattca
     1801 gggcgagatc gaaatccgcg atgccagcga actgcgatta aaggagagcg atcgacttgc
     1861 gaccacagcg cacaacctgc gcgctatggg tgctgatgtc gaagaactac ctgacggact
     1921 gcgcatctcg ggcccaaccc aacttcacgg cgcagtgatc gactcatacg gtgatcatcg
     1981 gatagcgatg gccttctctg tcgccgcact tctcgcctcc agtaacaccg aaatccgtgg
     2041 ctctgaatcc gccggcgtct ccttcccgga gtttttcggt ctgctcgact cgctaacgga
     2101 aagatgaaac ctgtcgcgcc aatcattatc acgggattta tgggttgcgg taaaactgag
     2161 actgcacgcg cactcgccaa ccgccgcggg ctggcgttga cggatctcga tcgcgcgatc
     2221 ttcaaccaaa cagggcgaac tgcggcccaa ctcatcacac aagaaggcga gcaggcgttt
     2281 cgtgcaatag aaacaaagat gttgcgcgaa ttattgttga accgcggaac gggcgtgatt
     2341 gcgcttggcg gtggcgcatg gatcacggac gagaatcgtg agttgattag cgagcacaac
     2401 ggtctgagtg tctggctgga tgttcctttc gaactgtgct ggcaaaggat tgccacttcc
     2461 gccgaagtta ggccattggg ccgcacacgc gaagacgcgg aaaaactcta tcttctgcgc
     2521 cggcccatct atcaacttgc tacgattcac ctgccggtcc gggccgacga acctctcgaa
     2581 aatatcgtcg cccgtctcga agctgcgctc gcaagctacc accgcgacag aaacgagcgt
     2641 agcgactgat ccattttgtc gaatgactcg gcgcaaaata agatcgcctg gtaatgcgtc
     2701 gggtcgtaca cgtgattcgc agccgcttcc aaatcaagcg gtcgcaactc cgccttgtgc
     2761 atctcggcca gttcaccagc cgacgacagc agaccagtgc cgtaagcttt gatctcaccg
     2821 ttctcccgca aaaccccaaa ttcaatcgtg aaccaataaa ctcgcgccaa acggtcgagt
     2881 tcgctcgatg acgcagtgcg ccggacagct tcgccaaaca tgcgattgat ctcagcgaac
     2941 cgcttgctcg ccagagtcac agcgtggccg atgatctcgt gcgccacgtc cggctcgggc
     3001 gtgtacaaag gcgtcgagtg gtggcgaatg tactgcgtgc acagaaacac tcctcgcgcc
     3061 agcgactcga gaaatatccg cggctcgacc agtcccgcca caggttccag ccgaaagccg
     3121 ctaatcgctt ccaccttccg gctcacctcg tccaactgtg gaattcgatc agtgtcgaag
     3181 ttcaatttct cgcgttggca aattcgcagt tagccaacgc tttcagcgca aaaggtaaaa
     3241 acatccaatc aatccccagg gttgtcgcac tccaacct
//
LOCUS       DASTEY010000656         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_64353, whole genome shotgun sequence.
ACCESSION   DASTEY010000656 DASTEY010000000
VERSION     DASTEY010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64353"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFO72_11450"
     CDS             <1..611
                     /locus_tag="VFO72_11450"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9479955.1"
                     /translation="SASLDELIRLYDADKTKEIRFAVIRALAERQDPRARTKIFEIAR
                     QGETPELRIEAIRRLGERRMALDDLLQLYTGETNVQIKQGLLRAFADNADPRARTKLL
                     EIARGNEAIELRGYAIRRLGEKDDEQTVNQLVAMYDTEQNAQVRNALMRAFGDSKQKS
                     AVRKLMTIARNDPSVELRKAAVRYLGESKDPEALKFLEDLLK"
     gene            658..2199
                     /locus_tag="VFO72_11455"
     CDS             658..2199
                     /locus_tag="VFO72_11455"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9479956.1"
                     /translation="MIRAAILLFALVGFVAAADELQTQSPTTTVQSFTAVEGADLMAK
                     LEAAQTRARARQTPYWSAYTFDVRSGVAIDPAIRDFNGSINTMGETTVFIGTTSTGLT
                     VETRNLAIFLLRDPASNQITRMEVFNLERKREYSGYPVYWLGRANNEESLNYLRAIAA
                     ATPLDMLSERAVLSIALHDDARVSGMLKNFIETSPNQRIRSTSVYWLGQVGGEQTFLA
                     SLVRNESEDNKIRHSAARAIGHSRDRGAVTTLQGLYESLKDAPLRRSVVAAVGNSIDQ
                     QPAYAFLLGVAKNDPDWESRRTAVKQMGRFQREDALEELMKIYNNDANVEVKRAALRS
                     MAETKNPRAQARLLEIARTATHPELRKTAIRALSDRGEAAVDDLLKLFDSETATDVKR
                     TVLQSLSDIKSARAEDKLFEVARGNDPVDVRRQAIRLLGERVGKRSFDFLSQTAQSST
                     GDAEVQVQAVRAISQRRAEESVPLLIKIAKTHPNQLVRKQAIRSLGESGDPRAVDFFR
                     EVLRN"
BASE COUNT          532 a          685 c          665 g          437 t
ORIGIN      
        1 attccgcttc actcgacgag ttgatccggc tctacgacgc cgacaaaaca aaggagattc
       61 gttttgcggt tattcgcgcg cttgccgaac gtcaagaccc gcgcgcacgc accaagatct
      121 tcgaaatcgc gcgccagggc gaaacgccgg agctgcgcat cgaagcgatc cggcgccttg
      181 gcgagcgccg catggccctc gacgatctgc tccagctcta caccggcgaa acaaacgtgc
      241 aaatcaaaca aggcctgctg cgcgcttttg ccgacaacgc cgatccgcgc gcacgcacta
      301 agctgcttga aattgcccgc gggaacgagg cgatcgagct gcgcggctat gccattcgac
      361 gattaggcga gaaagacgac gagcaaaccg tcaatcaact tgtggccatg tacgacaccg
      421 agcagaacgc gcaagtcagg aatgcgttga tgcgagcctt tggcgattcg aaacagaaga
      481 gcgccgtgcg caagctgatg acgatcgctc gtaacgatcc atctgtggag ctacgcaaag
      541 cagccgtcag gtatctgggc gagagcaaag atcctgaagc gctgaagttt cttgaagacc
      601 ttttgaagta gtaggtccta taggacctat ttggacctat tttcatctgg aggaatcatg
      661 attcgcgcag ccattctact tttcgcgctg gttggattcg tcgctgccgc cgacgaactg
      721 caaacccaat cgcccacgac cacggtgcaa agcttcaccg ccgtcgaagg cgcggacctc
      781 atggccaaac ttgaagccgc gcagacgcgt gcgcgcgcgc gtcaaacgcc ctactggtcc
      841 gcttacactt tcgacgtgcg ttcgggagtc gcaatcgatc ctgctattcg tgactttaac
      901 gggtcgataa acacgatggg cgaaaccaca gtcttcatcg gcacgacctc gaccggattg
      961 actgtcgaaa cacgcaacct cgccatcttc ctgctgcgcg atccggcgag caatcagatc
     1021 acgcgcatgg aggtcttcaa cctcgaacgc aaacgcgagt acagcggcta tcccgtttac
     1081 tggctcggcc gcgcaaacaa cgaagagagc ctgaactacc ttcgcgccat cgccgccgct
     1141 acaccgctcg acatgttgag cgaacgcgcc gttctcagca tcgccttgca cgacgacgcg
     1201 agagtcagcg gcatgctcaa aaacttcatc gagacatctc ccaatcaacg catccgctcc
     1261 acctccgttt actggctggg ccaggtcggc ggtgagcaaa catttctcgc gtccctcgtg
     1321 cgaaacgagt cggaagacaa caagattcga cattctgcgg cacgtgcgat cgggcatagc
     1381 cgcgaccgcg gcgccgtcac cactctccag ggtctttacg aaagcctcaa ggacgctccg
     1441 ctccggcgca gtgtagtcgc ggccgtgggc aatagcatcg atcagcaacc cgcgtacgcg
     1501 tttctgctgg gtgtggcgaa gaacgatccc gactgggaat cgcgtcgcac ggcggtaaaa
     1561 cagatgggac gctttcagcg cgaagatgcg cttgaagagt tgatgaagat ctacaacaac
     1621 gacgcgaacg tcgaggtaaa gcgcgcggcg ttgcgttcga tggcggagac gaagaaccca
     1681 cgcgcgcagg cacggctgct tgagatcgcg cgcaccgcta cgcatcccga gttgcgcaag
     1741 acggcgatcc gtgcactgag cgatcgcggt gaagcggcgg ttgacgatct gctgaagttg
     1801 tttgattccg aaactgcgac ggacgtcaaa cggacggtgc ttcagtcgct ttcggacatc
     1861 aaaagcgcgc gcgctgaaga caagctgttc gaagtagcgc gcggcaacga tccggttgac
     1921 gtgcgacgac aggcgatacg cctgctcgga gagcgcgtgg gcaaacgcag cttcgatttt
     1981 ctgagtcaga cggcccaaag ctccactggt gatgcagagg tacaggtgca ggcggtgagg
     2041 gcgatcagcc agcggcgtgc tgaagagtca gtgccgctgc tgatcaagat tgcgaagacg
     2101 catccgaacc agctcgtgcg caagcaggcg atccgttcgc tcggtgaatc cggcgatcca
     2161 cgcgcggtgg acttctttag agaagtcctg aggaattaat aggtcctata ggacagatag
     2221 gacctattac ttctcgatcg cgaagtcggc ccattgggcc actgccggtc ccttttgctt
     2281 tacattcctg tttcttacaa ccacctgaag atgataatg
//
LOCUS       DASTEY010000657         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1333561, whole genome shotgun sequence.
ACCESSION   DASTEY010000657 DASTEY010000000
VERSION     DASTEY010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333561"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3290)
                     /locus_tag="VFO72_11460"
     CDS             complement(<1..3290)
                     /locus_tag="VFO72_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9479957.1"
                     /translation="MSSVEPEGVLLHYRILSKIGEGGMGQVYKAEDTKLGRHVALKLL
                     APESNRDVTAKRRLLSEAQSASVLSHPNIVTVYAIEEVNGRDFIVMEFVEGETLSAHL
                     ALNGALPLPTLLDVGIQIADALQAAHASGIIHRDIKPGNILITPKGVVKVTDFGLAKQ
                     MRVTSGTIDREALTLAANLTGPGIVLGTAAYMSPEQTRGETLDLRSEIFSLGAVLYEA
                     ATRTRAFAGPSMLSIMHAIAATDPPPPSKLRPELPREFDLIIERALAKNRELRYSSAA
                     EMGDALRSLRASLSGTWAGFPIVYDSDLVDRSAPSFVGREPEMRKLEGLLDKAIEGTG
                     RVVFVTGEPGIGKTSLGDEFLRRTRKQRPGLLISRGRCVEQYGTGEAYLPFLDAMGGL
                     LQAPGRERIAAVMRTYAPTWCMELPTAFASSGSLEKLQQETIGATKERMMREMGDALG
                     ILSTSSPIVLLLEDLHWADPSSVDLLRHLCQRINTQRLLIAGTFRPEDVERSGHPLKS
                     YKAEMMAHNLCEEIALGSWTREHIAEYVDATFAPNDFPAELTSLIHEKTEGHPLFAAN
                     FLQYLSERGDLSKANNRWSLVRPLAEMDLEAPESVRGMISKKIDVLDAEERRALQYAS
                     VEGTEFHSTVTAKLLGVDEIDLEERLAEIAKTHRLIEILGEEELPDGSLITRYRFSHA
                     LYQNFLYGDLVNKRRIVLHQQAGEQLLKHFGKRAPHIATQLAIHFERGRDFPRAVEYL
                     IHAGDNAAKLYGYAEAEKHYTQALRLVEKIADAHNEQLSTLYQKRGTVNHALSNFSQA
                     ADDFTKMLEQAQALGSLELQSAALNSLTMTLFFSHRLEETLTRASEALEVAERAGNEK
                     LRADTMLMIGLKHLCYGELKEGKVILDDVVATARRLDHKPALVGGVTWRACLHFFQSE
                     YELAVEKAKEGIRLASELRDGFLLLTAMFFLGLSQGNQGKMSAALATLQEALEKARRN
                     GDLFWHPRFPNCIGWIYRELHDFEAARKFNEEGVDVARLHNVLEAEANSLINRGIDCT
                     HAGQRECTDSAFAEVHEIFARDAWFRWRYNIRLQAATAAHYLRHGELDKAEEYIVRLE
                     KTAREHEV"
BASE COUNT          737 a         1086 c         1082 g          832 t
ORIGIN      
        1 tgaacctcgt gttcgcgcgc tgtcttttcc agccgcacga tgtactcctc ggctttgtcg
       61 agttcgccat gacgcaggta gtgcgcagcc gtggcggctt gcaggcggat gttatagcgc
      121 caacggaacc acgcatcgcg cgcgaatatc tcgtgcactt ccgcaaatgc tgagtcggtg
      181 cactctcgct gccctgcatg ggtgcaatca atgccgcggt tgatcaacga gttcgcttcc
      241 gcttcgagca cgttgtgcaa ccgtgcgacg tccacgcctt cctcattaaa cttgcgtgcg
      301 gcttcgaagt cgtgtaactc gcgatagatc cacccgatgc aattcgggaa acgcgggtgc
      361 caaaacagat cgccatttcg acgcgctttt tccagcgcct cctgaagtgt ggccagtgcc
      421 gccgacatct tgccctgatt gccctgcgaa agtccgagga agaacatcgc cgtcagcaaa
      481 agaaaaccgt cgcgtaactc cgaagctagt cgaatgcctt cctttgcctt ctcaaccgcc
      541 agttcgtatt ccgattgaaa gaagtggaga caggcacgcc aggtgacgcc accgacgagg
      601 gccggtttgt gatcaagccg gcgcgccgtc gccactacgt cgtcgagtat gaccttgcct
      661 tccttcaatt cgccgtagca aagatgcttg agtccgatca tcagcatggt gtccgcgcgc
      721 aacttctcgt tgcccgcgcg ctcggcgact tcgagcgctt cgctcgcacg cgtaagagtt
      781 tcttcaagac gatgcgagaa aaagagcgtc atcgtcagcg agttgagtgc ggctgactga
      841 agctcgagcg agccgagcgc ctgcgcctgt tcgagcatct ttgtgaaatc gtcggctgcc
      901 tgcgagaagt tactgagtgc gtggttcacc gtgccgcgct tttgatacag cgtgctcaac
      961 tgttcgttgt gcgcgtccgc aattttttcg acgagacgaa gcgcctgcgt gtagtgcttt
     1021 tcagcttcgg cgtagccgta gagtttggct gcgttgtcgc cggcgtggat caggtattcg
     1081 accgcacgcg gaaaatcgcg accgcgttcg aagtgaatgg caagctgcgt tgcaatgtgc
     1141 ggcgcgcgtt tgccgaagtg cttcaggagt tgttcgcctg cctgctggtg caacacgatg
     1201 cggcgcttgt tcaccaaatc gccatacaga aagttctgat aaagcgcatg cgaaaagcgg
     1261 tagcgtgtta tgagcgaacc gtcaggcagt tcctcttcgc cgagtatttc tatcaagcga
     1321 tgagttttcg cgatctcggc cagccgttct tcgagatcga tctcgtctac gccaagcagt
     1381 tttgccgtga ccgtcgaatg aaactcggtg ccttcaacac tcgcatactg caaggcgcgc
     1441 cgctcttccg cgtccaacac gtcgatcttt ttgctgatca tgccgcggac gctttccggc
     1501 gcttccagat ccatctctgc cagcgggcgg acgagcgacc agcgattgtt cgccttgctg
     1561 agatcgccgc gctcgctcag atactgcagg aagtttgcgg caaacagcgg atggccctca
     1621 gtcttctcgt gaatcagcga ggtcaactcg gcaggaaaat cgtttggcgc aaacgtcgca
     1681 tcgacgtact ccgcaatgtg ttcgcgagtc cacgagccga gcgcgatctc ctcgcagagg
     1741 ttgtgcgcca tcatctcggc tttatagctc ttaagcggat gcccgctgcg ttcgacgtcc
     1801 tctggccgga acgtgccggc gatgagcaat cgttgcgtgt tgatgcgctg gcaaaggtgg
     1861 cgcaggagat caacgctcga cggatcggcc caatggagat cttcgagtag cagaacgatc
     1921 ggtgacgagg ttgagagtat gccaagcgcg tcgcccattt cgcgcatcat tcgttctttc
     1981 gtcgcgccga tcgtttcttg ttgtagtttc tccagcgaac cactcgaagc aaaagccgtg
     2041 ggcaactcca tgcaccacgt cggcgcgtaa gtgcgcatga cagcggcgat acgctcgcga
     2101 ccgggcgcct gtaacagacc acccattgcg tcgaggaaag gcaggtaagc ctcgccggtg
     2161 ccatactgct cgacgcagcg accgcgagag atcaagagtc cgggtcgctg tttgcgcgtg
     2221 cgacgcagaa actcgtcgcc gagtgatgtt ttgccgatgc ccggttcgcc ggtaacaaac
     2281 acgacgcgac cagttccttc aatcgcctta tcgagcagac cttcgagctt ccgcatctcc
     2341 ggttcgcggc caacgaagga gggcgcagaa cgatcgacca gatctgaatc gtagacaatc
     2401 ggaaagccgg cccacgtacc actcagcgac gcgcgcaaac tacgcagggc gtcacccatt
     2461 tcggcggcgg agctgtaacg cagttccctg ttcttcgcga gcgcgcgctc gatgattaga
     2521 tcgaactcgc gtggcagctc gggtcgcaac ttgctcggcg gcggcggatc tgtcgcggca
     2581 atcgcatgca tgatcgacaa catgcttgga ccagcgaagg cacgggtccg ggtcgcggct
     2641 tcgtagagca cggcgccgag cgaaaagatc tcggaacgaa gatcgagcgt ttcaccgcgc
     2701 gtctgttccg gcgacatgta agccgcagta ccaagcacga tgcctggtcc agtcagattc
     2761 gctgccagag tcagggcttc gcgatcgatt gtgccgctgg tgacgcgcat ctgttttgcg
     2821 agcccaaagt ctgtaacctt cactacgcct ttcggcgtga tgagaatgtt gccaggcttg
     2881 atgtcgcgat ggataatgcc acttgcgtgt gccgcttgaa gcgcatcggc gatttgaatt
     2941 ccaacgtcaa gcaacgttgg gagcggcaac gctccgttga gtgcgagatg tgcgcttaaa
     3001 gtctcgcctt caacaaactc catgacaatg aagtcgcgtc cgttaacttc ttcgatggcg
     3061 tagacggtga cgatgttcgg atggctcaat actgaagccg attgtgcttc ggacaacagg
     3121 cggcgtttgg cggtaacatc tctattggat tcgggcgcga gcagcttcag ggcgacgtgg
     3181 cggccgagtt tggtgtcctc cgctttgtat acctggccca ttccgccctc gccgatcttg
     3241 gagaggatgc ggtagtgcag caggacacct tctggttcca ctgagctcat taattgttac
     3301 gcgtatttcc gggggattcc gggccgcata atataagatc accgacgata ttgactaccc
     3361 tcgccacatg aggagtagcg atgcagattt ccgggacgtg ctcttccctg ggaccgcagg
     3421 cgtctcgcct gcctgtcacg gcagcgcccg attcgtcacc gtgcaggcga gacgcctgcg
     3481 gtcccaccaa gacggtgtct gcggtccccg caagacggcg cctcagattt caaatgcgtt
     3541 actgcggccg tccgtcaaga gtcctgtttc agacggacct tgaggcaaag cacgttacta
     3601 ccttgccggg ggcttctgct tgtttgtagt tgtcgtttgc tctattatgc gcgcgttgct
     3661 ctaacgtacc caaacactgg tcttctgagt ctgaaccaga accgagagac cgtagcgtag
     3721 aacagcaaac ctcgctg
//
LOCUS       DASTEY010000658         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1235041, whole genome shotgun sequence.
ACCESSION   DASTEY010000658 DASTEY010000000
VERSION     DASTEY010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235041"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1898)
                     /gene="uvrC"
                     /locus_tag="VFO72_11465"
     CDS             complement(6..1898)
                     /gene="uvrC"
                     /locus_tag="VFO72_11465"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9479958.1"
                     /translation="MSIEEKLKEIPSSPGVYLHKDAAGKVIYVGKAKNLRSRVRSYFR
                     ARPFDRKTDALVGQITDFEFIVTDNEVEALILEATLVKQHKPRYNVKLQDDKSYPHLK
                     LTINEPFPKVVITRRVQKDGALYFGPFLPASLARRTIDLINRTFQLRTCDIEIDGKAP
                     RPCLEYHIKRCLGPCVKGLCTPEQYQEAVRDVRLLLEGRNKELADALESRMFEASDDM
                     RYELAAKYRDLRKTVIRLSEQQKMATTSESDVDIFGYYREGQRLALQLFTMREGNIVG
                     RREFFWEDLTEDVFDPAEFLSDVLAQYYASDYVAREIYVPVDFHDRELLEKALSKRRG
                     RRVHIEVPQRGEKRDMIDLVDKNAKLAFEQRFRVLKPDMKRVLEELQETLELPHFPAR
                     IESFDISHIQGAENVAGMVVCDNGKMNRAEYRKFRIRSVDGANDVASMREVVTRRYRR
                     LLEEGKPLPDLIMIDGGKGQLNGAAEAMSELGLEAIPMVGIVKPPQRHNDVAYLLMKG
                     REDVPVYLDSHSLILRLLQMIRDETHRFAVTYHRKRRELRDFTSELTAIPGVGDKRKG
                     RLLRHFGSIQRISRAGVSELTPFVGRKTAEEIAEHFERQRLLAQQAVTTDNTDQVASP
                     RTPLNS"
     gene            1929..2870
                     /locus_tag="VFO72_11470"
     CDS             1929..2870
                     /locus_tag="VFO72_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9479959.1"
                     /translation="MKFPIEARANKRFDAVGFGLNAVDHLIVVPEYPAFDTKIRLLAH
                     KQSAGGQTATAMVALRRLGLKTAYAGRFGSDPEGQFGLKSLAGEDVNVDFAEVVEGAT
                     NQIAFITIDARSGERTIVWDRDERLAYKPEEAPVEFASLGRVLHLDAHDPAACVVLAR
                     AAKQSGAIVSADIDNVYEGLPELLPLIDILIGSKEFPHRVTGITDERATLVELHERYG
                     CAVVGMTIGAAGAVVYCEGEFIESRGYDAPGGCRDTTGAGDAFHGGLLYGFLTGEDLE
                     TSLMFANAVAAMKCSALGARTGLPHRSELENFLNNSC"
     gene            2898..4562
                     /locus_tag="VFO72_11475"
     CDS             2898..4562
                     /locus_tag="VFO72_11475"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET9479960.1"
                     /translation="MSVLLLTFVPLQPRVAPADLVFKNGNIYTANDRAPQAQAIAVKA
                     DRIVFVGSNAAAQRYVGANTRVVDLSGKTVLPGFTDSHQHLSGVGLREMTLNLEGTTS
                     LEDLLAKLKARVDQTKPGEWVTGRGWIETHWQPPVFPTRWDLDKVSPNNPVILGRADG
                     HGSVANSAALKLAGVDKNTPDPFGGEISKDKQSGEPNGMLLDSAQGLVRRKVPPTSAE
                     DAERAVVLGVKRNIELGWTQIQDAGGGYNDVEIFKKLYTSGAIKLRIYKAVHGPGPSA
                     TRLLNEGPTIGAFDNRFTFRTIKVVSDGALGSRGAALLAPYSDAPDTSGFLTVKAEEL
                     RPMLIEALRKGIQVETHAIGDRANRFVLDEYEAALKAVQPAERKVADPRWRVEHAQIV
                     NPADIPRFARLGIIPSMQPSHAIGDLFFAPSRVGISRLAGSYAWESFIKSGIVVPGGS
                     DAPVERGEPMIEFYAAVARKDQKGFSGEGWHPEEAITRAQALKMFTLWPAYAAFEEKL
                     RGSIEVGKLADLTILSADIMKIPEPEILKTRCVMTVINGEIVFGQG"
     gene            4570..>5075
                     /locus_tag="VFO72_11480"
     CDS             4570..>5075
                     /locus_tag="VFO72_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9479961.1"
                     /translation="MKTDRRKFLSLAALSVPSIGALLKEIEAATRSVAHLTPEEAAMN
                     EDYWSIIQNSFSVTRGIINLNNGGVSPSPRIVTEALVRYIWQQEDATAYTMWQILEPQ
                     SETIRTGLAEMFGCDREEIAITRNASESLEILLMGMDFKPGDEILTTTQDYPRMLTTL
                     RQRERREGL"
BASE COUNT         1082 a         1381 c         1490 g         1122 t
ORIGIN      
        1 ttgatctacg agtttagcgg cgtgcgtggc gaagcgactt gatctgtgtt atcggtggta
       61 actgcttgtt gagctaacaa tcgttggcgc tcgaaatgct ccgcgatctc ttccgccgtc
      121 ttgcggccta caaacggagt caactccgaa actccagcgc gcgagattcg ttggatcgac
      181 ccgaagtggc gcagcagtct tcctttccgc ttatcgccca cacccggaat agccgtcagc
      241 tcggaagtga agtcgcgcag ttcgcgccgt tttcggtggt aagtgactgc gaagcgatgc
      301 gtctcgtccc gaatcatctg caacagacgc aagatcagcg agtgggaatc aagatatacg
      361 ggcacgtcct cgcgtccttt catcagcagg tacgcgacgt cattatgtcg ttgcggcggc
      421 ttgacgatgc ccaccatcgg aatggcttcg aggccaagtt cgctcatcgc ttccgctgct
      481 ccatttagtt ggcccttgcc gccgtcgatc atgatcaggt ccggaagcgg tttgccttct
      541 tcgagtaacc ggcggtaacg tctggtcacc acttcgcgca tcgaggcaac gtcgttcgcg
      601 ccatcaacac ttctgatacg gaactttcga tactccgccc ggttcatctt gccgttgtcg
      661 caaacgacca tgccggcgac gttttccgcg ccctgaatgt gggagatatc aaacgactcg
      721 atgcgcgctg gaaagtgcgg cagttcgagc gtctcctgca actcttcgag cacgcgcttc
      781 atgtcaggct tgagcacgcg gaaacgctgc tcaaacgcga gcttcgcatt tttgtcgacg
      841 agatcgatca tgtcgcgctt ctcgccacgt tgcggcactt cgatgtgcac gcgacggccg
      901 cgacgtttgc tcaaagcttt ctcgagcaac tcgcggtcgt gaaaatctac gggtacgtag
      961 atctcgcgcg ccacgtaatc cgacgcgtag tactgcgcca acacatcgct caaaaactct
     1021 gccggatcga aaacgtcttc cgtcaggtct tcccagaaaa actcgcggcg cccgacgatg
     1081 ttgccctcgc gcatcgtgaa caactgcaag gcgagccgtt gtccttcgcg atagtagccg
     1141 aaaatatcaa cgtcgctttc ggacgtcgtt gccatcttct gttgttcaga aaggcgaatc
     1201 acagttttac gcaggtcgcg atatttagcg gccagctcgt agcgcatgtc gtccgacgct
     1261 tcgaacatgc gcgattcgag cgcgtcggca agttccttgt ttcgtccttc caacaagagt
     1321 cttacgtcgc ggactgcttc ctgatattgt tccggcgtgc agagtccttt cacgcatggg
     1381 ccaaggcaac gtttgatgtg atactccaga cacggccgcg gtgctttacc gtcgatctca
     1441 atgtcgcagg tgcgcaattg gaatgtgcgg ttgatgagat cgatcgtgcg acgcgctaaa
     1501 ctcgcgggca gaaacggtcc gaagtagagg gcgccgtctt tttgcacgcg acgcgtaatg
     1561 acgaccttgg gaaacggctc attgatggtg agcttcaggt gcggatacga cttgtcatct
     1621 tgaagtttga cgttgtaacg cggcttgtgt tgtttgacga gcgtcgcttc taaaatcagc
     1681 gcctcgactt cgttgtcggt tacgatgaac tcgaaatcag tgatctggcc gaccagcgcg
     1741 tcggtcttgc ggtcgaaagg gcgggcacga aagtaggaac gcacacggct gcgcaggttc
     1801 tttgctttgc cgacatagat cacctttcct gccgcgtctt tgtgcaggta aacgccgggc
     1861 gaggaaggta tttctttgag tttctcttct atcgacacga tattcagatg ccattttaac
     1921 ttaccgagat gaagtttccg attgaggcac gcgccaacaa acgtttcgat gcggtgggtt
     1981 ttgggttgaa cgcggtcgac catctgatcg tcgtgcccga gtatccggcg ttcgacacga
     2041 agatccgttt gctggcgcac aagcagtctg cgggcggaca gacagcgacg gcgatggtag
     2101 cgctgcgccg cctcggtttg aagactgctt acgccgggcg cttcggatcg gatccggaag
     2161 gacagtttgg tttgaaatcg ctggccggcg aagatgtcaa cgtcgacttt gccgaggtag
     2221 tcgaaggagc aacaaaccag atcgccttca tcacgattga cgcgcgcagt ggcgaacgca
     2281 ccatcgtttg ggatcgtgac gaacggcttg cttacaaacc ggaagaagcg ccggttgaat
     2341 ttgcgtcgct ggggcgggtg ctgcatctcg acgcgcacga tccggcggcg tgtgtggtgt
     2401 tggcgagggc cgctaaacag agcggtgcga tcgtttccgc cgatatcgat aacgtttatg
     2461 aaggtcttcc tgaactgctt ccgctgatcg acatcctgat cggctcgaaa gagtttccgc
     2521 atcgcgtcac cggcatcact gacgaacgcg ccacactggt tgaactgcac gagcgatatg
     2581 gttgtgctgt ggttggtatg acgattggag ccgcgggcgc ggtcgtctat tgcgaagggg
     2641 agttcattga atcacgcggc tacgatgcgc cgggcggttg ccgcgacacc accggcgccg
     2701 gcgatgcatt tcacggcgga cttctctatg gatttctcac cggtgaagat ctggaaacga
     2761 gtttgatgtt tgcgaatgcg gtagcggcga tgaagtgtag cgccttaggc gcacggactg
     2821 gtctgccaca cagatcagaa ctcgaaaatt ttctcaataa ttcgtgttaa tctgtggcta
     2881 tgaaacccct cttaatcgtg agcgttttgc tcctgacctt cgtaccgctt cagccgcgcg
     2941 ttgcgccggc cgacctggta ttcaaaaacg gcaacatcta caccgctaac gatcgcgcac
     3001 cgcaggcgca ggcaatcgcc gtcaaagccg atcgcattgt ttttgtcggt tcgaacgcag
     3061 cggcacaaag atacgtcggc gcgaacacgc gcgtggtcga tttgagtggc aagaccgtgc
     3121 tgcccggttt caccgattca catcaacatc tctccggcgt cggtctgcgt gagatgactt
     3181 tgaacctgga aggcacgacc agcctcgaag acctgctcgc gaaactaaaa gcgcgagtag
     3241 accagacgaa acctggagaa tgggtaactg gacgcggctg gatcgaaacg cactggcagc
     3301 cgcccgtctt cccgacacgt tgggacctcg acaaagtctc gccgaacaat cccgtgatcc
     3361 tcgggcgcgc ggatggacac ggttccgtgg caaacagcgc cgcgctgaaa ctggccggcg
     3421 tcgataagaa cacacccgac ccattcggcg gcgagatctc gaaagacaaa cagtcaggcg
     3481 aaccaaacgg catgttgctc gactccgcgc aggggctcgt ccggcgcaag gtaccgccga
     3541 cttcagcgga agatgcagag cgcgcggtgg tgttgggtgt caaacgaaac atcgaacttg
     3601 gctggacgca gattcaagat gcgggcggcg gttacaacga cgtcgagatc ttcaagaagc
     3661 tttacacgtc gggcgcgatc aagttgcgca tctacaaagc cgttcatggt ccaggcccga
     3721 gcgcaacgcg cctgctgaac gaaggtccaa ctatcggcgc attcgataat cgatttactt
     3781 ttcgcacgat caaagtagtg tctgacggtg cactcggttc gcgcggcgca gccctgttgg
     3841 ctccgtattc tgacgcgcct gatacctccg ggtttttgac ggtcaaagcg gaagagttgc
     3901 ggccgatgct gatcgaagct ttgcgcaaag ggattcaggt ggagacgcat gcgattggcg
     3961 atcgtgcgaa tcgtttcgtt ctcgatgagt acgaagctgc gttgaaagcc gtgcagccag
     4021 ccgaaagaaa agttgccgat ccgcgttggc gcgtggagca tgcgcagatt gtgaatcccg
     4081 ccgacattcc gcggttcgcc aggttgggaa tcattccgtc gatgcagccg tcgcacgcca
     4141 tcggcgatct gttttttgcg ccgagccggg ttggcattag cagattggct gggtcgtatg
     4201 catgggagag tttcataaag tcgggaatcg ttgtgcccgg tggatctgat gcgccggtag
     4261 aacgtggcga gccgatgatt gagttttacg cggccgtggc gcgcaaggac cagaagggtt
     4321 tttcgggcga aggatggcat ccggaggaag ctatcacacg ggcacaggcg ttgaagatgt
     4381 ttacgttatg gcccgcttat gcggccttcg aagagaaact gcgtggttcg attgaggtag
     4441 ggaagctggc ggatttgacg atcctgtcgg ccgacataat gaagattccg gagccggaaa
     4501 tattgaagac gcgctgtgtg atgacggtca tcaatggcga gatcgttttt ggacagggat
     4561 gaaagtccga tgaaaacaga tagacgcaag tttctgtctc tggcagcatt atcggtgcca
     4621 agtatcggcg cgctgctcaa agagatcgaa gcggccactc gcagcgttgc gcatctcacg
     4681 cccgaagagg cggcgatgaa tgaagactac tggtcgatca ttcaaaactc gttctcggtt
     4741 acgcgcggca tcatcaacct gaacaacggc ggcgtttcgc cgagtccacg catcgtcacc
     4801 gaagcactgg tccgttacat ctggcaacag gaagacgcaa ccgcctacac aatgtggcaa
     4861 atactcgagc cacagtcaga aacaattcgg accggcctgg ccgagatgtt tggctgcgat
     4921 cgcgaagaga tcgccatcac tcgtaacgcc tcggagtcgc tcgagatcct gctcatgggc
     4981 atggatttca aacccggcga tgaaatcctt accaccactc aagactatcc gcgcatgtta
     5041 accacgttgc gccaacgcga acgccgcgag ggact
//
LOCUS       DASTEY010000659         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_871890, whole genome shotgun sequence.
ACCESSION   DASTEY010000659 DASTEY010000000
VERSION     DASTEY010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871890"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2298
                     /locus_tag="VFO72_11485"
     CDS             <1..2298
                     /locus_tag="VFO72_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479962.1"
                     /translation="MLRRLPYRTADRIVVIQELNPDGKRIQVTGPNFLDWRAQNTVFE
                     HLAAIKTRNANLTFADQAERIDMAQTSADFFDVFGAGPQHGRLFVSGDEQAGHAPVAV
                     LSDALWRRRFAGDTSAIGKTITLDGNTYTIIGIAPAGFQYPDKTEVWLPPLKLAPEFN
                     PRVEPTERRGMGYLSAVALLKPGVTVAQAASEMETITSRLRQQYPETNNRRFNRVLSL
                     HDHLIGDSRTMLWLLFGSVMFVLLIACANVANLLLASAASRSKEIAIRAALGASRWRV
                     IRQLLTESAILALAGGLLGLLFSSYGVKAITKLLPQEFPRLNEIDMDWRVFGFTLGAS
                     LLTGFVFGFAPALHISRTDVQEAMKESGRGTAGSARHTRLRHALIVAEVALSVVLLAG
                     AGLLFRSFMRLQAVNAGFTPQQVLTAQLSPAGPNFDDNGDYVVFYDQVLERARAIPGV
                     TSVGVINTLPLSGGPTIAFRVEGRPILTVDKWIPTSFRSVSPDYFRAMNIMVLKGRTF
                     TPEDNADAPRKLMINQALAQRDFPNEDAIGKRITLGNTDANNQPVWFEIVGVTANVRS
                     VELREEAPPELYFAARQLPFRTMALVVRSTVEPESLAPALRQSVAEVDRTVPVSEVQT
                     MEHIVSESVTQPRFNLFLLGLFGGIALLLSAAGIYGVTAYTVTQRTHELGIRLALGAQ
                     VTDVLKLVLGQGMAVIGIGLVIGLAAAFALLRLMRSLLFGVGENDPLTFATITLVLLL
                     VALLACYVPARRATKVDPLIALRYE"
     gene            2326..>2775
                     /locus_tag="VFO72_11490"
     CDS             2326..>2775
                     /locus_tag="VFO72_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479963.1"
                     /translation="MNKLALDNRQLAMGNGQWVTVMGSIFKDLQFAIRSLIKRPGFTA
                     VAVITLMLAIGVNTTIFSVVNAVLLRALPFRGPEQLVAVYIPAGAEGLPGLAAYQYLA
                     WRERQTTFEDLGAFTDNNFNLTGHGEPERISCAQVTASLFNTLGVQPI"
BASE COUNT          555 a          793 c          803 g          624 t
ORIGIN      
        1 atgttgcgac gtctgccgta ccggaccgcc gatcgcattg tagtgatcca ggagctgaat
       61 ccggacggaa aacgaataca agtaaccggt cccaactttc tcgactggcg cgcgcagaac
      121 accgtcttcg aacatctcgc cgcgatcaag accaggaacg cgaacctgac cttcgcagat
      181 caggcagagc gaatcgatat ggcccaaacg tctgccgact tttttgacgt gtttggcgcc
      241 ggaccgcagc acggccgctt gtttgtgtca ggtgacgaac aagccggaca tgcacccgta
      301 gcagttttaa gtgacgctct gtggcggcgc cggtttgccg gcgacacgag cgctatcggc
      361 aaaacaatca cgctcgacgg caatacttac accatcatcg gtattgcgcc ggccgggttc
      421 cagtatcccg acaagacaga ggtgtggttg ccgccgctga aactcgcgcc ggagtttaat
      481 cctagagttg agcccaccga gcggcgcggt atgggttacc tcagcgcagt ggccctactt
      541 aaacccggcg tcaccgtggc gcaagccgcg agtgagatgg agacgatcac cagccggctg
      601 agacagcagt atcccgagac caacaatcga cgcttcaacc gcgtcctctc gcttcacgat
      661 catctgattg gcgattccag aacgatgctg tggctgctct tcggctcggt catgtttgtc
      721 ctgctgattg cgtgtgcaaa cgtcgccaat ctcctgctcg cgagtgcagc ttcacgcagc
      781 aaggaaatag cgattcgcgc tgcgctcggc gcgtcgcgct ggcgtgtgat acggcaactg
      841 ctgactgaga gcgcgatact cgcgctggcc gggggcctgc tcggcctgtt gttctccagt
      901 tacggggtga aggcaatcac aaagctgctg ccacaggagt ttccacgtct caacgagatc
      961 gacatggact ggcgcgtgtt tggtttcacg ctcggtgctt cgttgctcac tggatttgtc
     1021 ttcggctttg ctcccgcgct ccacatttct cgcactgacg ttcaggaagc aatgaaagag
     1081 agtggacgcg gcactgcggg cagtgcacgc catacacgtc tgcgacacgc attgatcgtt
     1141 gcggaagtcg cgttatcagt tgtgctgctc gcgggcgcag gactgctatt ccgcagtttt
     1201 atgcgcttgc aggcagtcaa tgccggtttc acgccgcaac aagtgttaac ggctcaactg
     1261 tctcctgctg gtccaaactt tgacgacaac ggcgactacg tcgtctttta cgatcaggtg
     1321 ctcgaacgag cgcgcgctat tcccggcgtt accagcgttg gcgtaatcaa tacgttgcca
     1381 ctcagcggcg ggcccacgat cgccttccgc gtcgaaggcc gtccaattct cacggtggac
     1441 aaatggattc cgaccagttt ccgcagtgtc agtcctgact acttccgcgc catgaacatt
     1501 atggtgctga aaggacgcac gtttacgccg gaagacaacg ccgatgcgcc gcgcaaattg
     1561 atgatcaacc aggccctggc gcagcgcgac tttcccaatg aagacgcgat cggaaaacga
     1621 atcaccttgg gcaacaccga tgcgaacaac caacccgtct ggtttgaaat cgtcggcgta
     1681 accgcaaacg tccgcagtgt cgaattgcgt gaagaggctc caccggaact gtacttcgcg
     1741 gcgcggcagc ttccgtttcg gaccatggct ttagtcgttc gttcgacagt tgagccggaa
     1801 agtttggcgc ccgcgctgcg ccagtccgtc gcggaagttg atcgaactgt gccggtttca
     1861 gaagtgcaga cgatggaaca cattgtgagc gagtcagtga cgcagccgcg cttcaatctg
     1921 tttctgctcg gacttttcgg cggcatcgct ttgcttttgt ccgctgcggg catctacggc
     1981 gtcactgctt acaccgtgac gcagcgaacg cacgagttgg gcattcgtct agcgctcggc
     2041 gcgcaggtga ccgatgtgct gaagctcgtg ttgggccagg gaatggcggt gattggaatc
     2101 ggtctcgtga ttggtctggc cgcagcattt gcattgctgc ggttgatgag aagtctgctt
     2161 tttggcgtcg gcgaaaacga tccactgacg tttgcgacga tcacgctggt gttactgctg
     2221 gtcgccttgc tggcttgtta tgttccggcg cgacgcgcaa cgaaagtcga tccgctgatt
     2281 gctctgaggt acgagtgatg gcattgccga ttgcattcag gtgacttgaa caaattggca
     2341 ctcgacaatc ggcaattggc aatgggcaat gggcaatggg tgactgtgat gggctcaatc
     2401 ttcaaggacc ttcagtttgc cattcgtagt ttgatcaagc gacccggttt tacggctgta
     2461 gccgtaataa cgttgatgct ggcgatcggt gttaacacaa ccatcttcag cgtcgtcaac
     2521 gcggtcctgc tacgagcttt accgtttcgc ggtccggagc aactggttgc cgtttacata
     2581 ccggccggcg ccgagggact gccgggcctc gccgcgtatc aatatctcgc gtggcgtgaa
     2641 aggcagacca ctttcgaaga tctcggcgcg tttactgata acaacttcaa cctcacgggc
     2701 catggtgagc ccgaacgcat ctcctgcgcg caagtcactg cgtctctctt caacacgctc
     2761 ggtgtgcagc cgatc
//
LOCUS       DASTEY010000660         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1465182, whole genome shotgun sequence.
ACCESSION   DASTEY010000660 DASTEY010000000
VERSION     DASTEY010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465182"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..765
                     /locus_tag="VFO72_11495"
     CDS             241..765
                     /locus_tag="VFO72_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479964.1"
                     /translation="MREALEAPDPAKKFEWGNTWVADMLMSGQAAEIRKLELERRMGI
                     TPEVKRYNEIKAGVIVSSVGVGLAIFLAILMQGVASHVDPKDAEIITRIWVAGVIPFM
                     VGLALIINGLVVSKKIVEAQERALKEGRAALDEGQTPRSLRPPDTSEFIPTNLSVTDG
                     TTRHLENAERKVNR"
     gene            762..1004
                     /locus_tag="VFO72_11500"
     CDS             762..1004
                     /locus_tag="VFO72_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479965.1"
                     /translation="MNRISGLEKSQAPWHLRWFYTTMRKMFGKDLTPVKQQMRLPGLI
                     WGSIAMEAGLGRKRKVSLRFIQLGKVRTAARIGCPF"
     gene            1041..1355
                     /locus_tag="VFO72_11505"
     CDS             1041..1355
                     /locus_tag="VFO72_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479966.1"
                     /translation="MTDEKLLAVVKNDLSVFSDVEKLVIELADAMANTPSNVSDELYA
                     RLREKFSEEQLLQLGGQIAFENYRARLNRIFDVRSDELYNPQMNADERGSNRRSSASS
                     AA"
     gene            complement(1341..>2317)
                     /locus_tag="VFO72_11510"
     CDS             complement(1341..>2317)
                     /locus_tag="VFO72_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9479967.1"
                     /translation="RNPGAVDLAHDQRPQHRLADSFATLVPELPDITVYIEALEQRIQ
                     GTTLERVRVASPFLLRTAVPPLSSVEGREVTDLRRLGKRICIGVEGDLWLVLHLMIAG
                     RLHWYDERAKAAKGRSVAVFEFSTGSLTLTEAGTQKRASLHVVQGEAGLDNLNPGGLE
                     IFDATTEEFARALRARNHTLKRALTDPRILSGIGNAYSDEILFEAKLSPFTLTQKLTD
                     KDIEQLFATIKRTLTDWVERLRSEAKDRFPEKVTAFRPDMATHGKYGEPCPRCGSKIQ
                     RIRYGSNETNYCPTCQTGGKLLADRALSRLLKDDWPRTPEELENLGRR"
BASE COUNT          508 a          592 c          706 g          511 t
ORIGIN      
        1 acgatccggt aatgcgcaat ggttttgatg ggagacggct tcatgtgttt ggggtcgacg
       61 acatgatgct gaacggctaa ttataacggc aacttcccct gacacgaacc gaggccgtaa
      121 aatcgtcgta tccgttagtg attccgaaca aaggagctgc ttatggtgtg tcctcgttgt
      181 gcctcgaacc aaaatgacga cataaagttc tgtacgtctt gcggagcgaa cctgcaagcg
      241 gtgcgcgagg cgttggaagc gcccgacccg gcaaagaagt ttgagtgggg taacacctgg
      301 gtcgcggaca tgttgatgtc gggccaggca gccgaaatac gcaagctcga gctggaacgc
      361 cgtatgggca tcacgcccga agttaaacgt tacaacgaaa tcaaagcggg cgtgatcgtg
      421 agtagcgtcg gtgtcgggct ggcgatcttt ctggccatct tgatgcaggg cgttgcgagt
      481 cacgtcgatc ccaaggacgc cgagatcatc acgcggattt gggtagcggg agtcattccg
      541 ttcatggtcg gtttggcgct gattattaat ggtctggtcg tgagcaagaa aattgtagaa
      601 gcccaggaac gagcgttgaa ggaagggagg gcggcgttgg atgaagggca gactcctcgt
      661 agtctgcgac caccagacac gagtgagttc attccaacga atctcagcgt taccgatggg
      721 acaacccgac atctcgaaaa cgcggaacgg aaagtcaatc gatgaacagg atttcgggat
      781 tagagaagag tcaggcgccg tggcacttgc gctggttcta taccacgatg cgcaagatgt
      841 tcggcaagga tttgacgccg gtgaagcaac agatgcggct gccgggactg atttggggca
      901 gcatcgcgat ggaggccggg cttggccgga agcggaaagt gtcgttacgg tttattcagc
      961 tcggcaaggt gcggaccgcc gcgcgcattg ggtgtccttt ttgagtggac atcaattctg
     1021 ccgtaggcag aaaagccggt ttgactgatg agaagttgct ggcagtggtg aagaatgatt
     1081 tatcagtgtt ctctgacgtt gagaaactgg tgatcgagct cgcggacgcg atggctaata
     1141 cgccttcaaa cgtttctgat gaattgtatg cgcgcctgcg cgagaagttt tccgaggagc
     1201 aactacttca gctcggcggc cagatcgcgt tcgaaaacta ccgcgctcgc ctcaatcgca
     1261 tcttcgacgt ccggagtgac gaactttata acccgcagat gaacgcggat gaacgcggat
     1321 ctaatcggcg ttcatctgcg tcatctgcgg cctaaatttt cgagctcctc aggggtgcgg
     1381 ggccagtcgt cctttaagag acgagaaagg gcgcggtccg cgaggagttt gccacctgtt
     1441 tggcacgtgg ggcagtagtt agtctcgtta gatccgtagc ggatgcgctg gatcttgctg
     1501 ccgcaacgtg gacacggttc gccatacttg ccgtgcgtcg ccatgtcggg acgaaacgcg
     1561 gttacctttt ccgggaaacg atccttcgct tccgaacgca aacgctcaac ccagtcagtc
     1621 aacgttcgtt tgattgtcgc gaagagttgt tcgatatctt tgtcggtgag tttttgtgtg
     1681 agcgtgaaag gcgagagttt tgcttcaaaa agaatctcgt ccgagtaagc gttgccgatg
     1741 ccgctgagaa tgcgtggatc ggtgagcgcg cgctttaacg tgtggttccg ggcgcgtaac
     1801 gcgcgcgcga actcttccgt cgtcgcgtca aaaatttcta agccaccggg attgagattg
     1861 tcgagaccgg cttcaccctg gaccacgtgc agcgaagccc gcttctgcgt acctgcttcg
     1921 gtcaatgtga ggctgccggt gctaaactcg aacacagcca cacttctacc tttcgcagct
     1981 ttggctcgct cgtcgtacca gtgcaacctg cccgcaatca tcaggtgcag aacgagccac
     2041 agatctcctt cgacaccgat acagatgcgc ttgccgagcc ggcgcaggtc agtgacttcc
     2101 cggccttcga cgctcgacag tggcggcaca gccgtgcgca gcagaaacgg actcgcgacc
     2161 cggacgcgtt ccagcgtagt gccttgaatt cgctgctcga gagcctcgat gtaaacggta
     2221 atgtccggca gttcaggcac taacgtcgcg aacgaatcgg ctagacggtg ctgcggcctt
     2281 tgatcatgcg ccagatccac agcaccagga ttgcgcc
//
LOCUS       DASTEY010000661         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_295613, whole genome shotgun sequence.
ACCESSION   DASTEY010000661 DASTEY010000000
VERSION     DASTEY010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295613"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..>2309
                     /locus_tag="VFO72_11515"
     CDS             275..>2309
                     /locus_tag="VFO72_11515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9479968.1"
                     /translation="MGAVYLSEDLHLARRVAIKFLTSTDRHYRARFIREARAVSALNH
                     PNIAMVHDYGETTDGQPFIVMEFVKGKSLSDLLEEGLTLRRSVEIVSAIAEALGEAHE
                     QGIVHRDIKPTNVLVSERGHVKVLDFGLVKHLFDAPVSDVDLDARTIYSTKTRSDVIV
                     GTPLYLSPEQATGKEIDGRSDLFALGALLYECLTGQSAFSGGSVLEIGAQIIHVTPER
                     PSKINPQVTPELDRITLKALEKNVEARYQTAAEMLKDLKAASQGLTGNGVPVSSRTDK
                     PTEGFKRATNAFATLTMSLKRQRFSLVSVIPAFVIAGLVIWGIIYFWPRTYYQPSSAA
                     LHWYNQGTDYLHNGAYYQASKALTQAIDLDSNYALARARLAQAWTELDYTDHAKDELL
                     AVTSITGDRSRVSPLDALYLDAINATVTRRFGDAIKFYGEIVKLTPESSPVYVDLGYA
                     YENDGNLDKALENYLKAIELNKGQYATAFLRAGIVYQRKGDTDKATARFDQAERLYQA
                     ASNNEGLNEVYRQRGILFRNNGLYDEAKKQFEQSLNAARSLGNEAQQITSLIGLSYLH
                     SSRGLYAEAENYAQQAVTIAQQKNLENLAANGLLEVGNSYSTKGDLPKAEQYWTQAIQ
                     LARVNKGRLTEHRGLTNLGGLYIQTLRIDEGLRMVQQAFAYFQQDNYPRIAGQC"
BASE COUNT          547 a          668 c          631 g          463 t
ORIGIN      
        1 aagagaagaa gattcgcgaa accgtccttt agccacaaag atgcacataa tctttagcca
       61 cagaaaggca cataagacac aagttttggg ctaaatcatt ttgtgccttg ggtgcttttt
      121 tgtggctcat tttttgaggc aaagcttggc gctgttcttg agtttaagcg tcctttagcg
      181 cacaatttgc ccgcaattcc acgtcctcgg acccacgatg attggccaga cggtctccca
      241 ttaccgaatc ctcgaaaagc taggcgaagg cggtatggga gcggtctatc tctccgagga
      301 tctccatctc gcgcggcgag ttgcgattaa gtttctgacc tcgactgatc gtcactaccg
      361 cgctcgcttc atccgcgagg cgcgcgccgt ctccgcgctc aatcacccaa acatcgcgat
      421 ggtccacgac tacggcgaga ccacggacgg ccagccgttc atcgtgatgg aatttgtaaa
      481 agggaagtcg ttgagcgacc tcctcgaaga agggctgacg ctgcgtcgct cggtcgagat
      541 cgtttcagca attgccgagg cgctcggaga agcacacgaa cagggcatcg tacaccgcga
      601 tataaaacct acgaacgtgc tcgtcagcga acgcggccac gtgaaggtgc tcgatttcgg
      661 cctggtgaag cacctgtttg acgctcccgt cagcgacgtg gatctcgacg cgcgcacgat
      721 ctactccacc aaaacccgca gcgacgtcat cgtcggaaca ccgctctatc tctcgcccga
      781 gcaggccacc gggaaagaga tcgacgggcg cagcgacctg ttcgcgctcg gagcgctgct
      841 gtatgagtgt ttgaccggac agtcagcctt ctccggcggc agcgtgctcg aaattggcgc
      901 gcagatcatt cacgtcacgc cggagcgtcc gtcgaagata aacccgcagg taacacctga
      961 gttggatcga atcacgctca aggcgctcga aaagaacgtc gaagcgcggt atcaaactgc
     1021 cgccgagatg ctgaaggatt tgaaagcggc gtcgcagggt ttgaccggca atggcgtgcc
     1081 ggtgtcgagc agaacagaca agccaaccga aggatttaaa cgcgcgacca acgcgttcgc
     1141 cacgttaacg atgtcgctga agcgccagcg gttctcgctc gtctccgtga ttcctgcatt
     1201 tgtcatcgcc ggactcgtga tctggggcat catttacttc tggccacgca cttattacca
     1261 gccgtcttcc gccgcgctcc actggtacaa ccaaggcact gactacctgc acaacggcgc
     1321 ctactaccag gcaagcaaag cgctcacgca agccatcgat ctcgacagca actacgctct
     1381 ggcgcgggcg cgtttggcgc aagcgtggac cgaacttgat tacactgacc acgccaaaga
     1441 cgaattgctc gccgtcacca gcatcactgg cgatcggtcc cgcgtttcgc cgctcgacgc
     1501 tctctatctg gacgcaatca acgccaccgt aaccagacgc ttcggcgacg caattaagtt
     1561 ttacggcgag atcgtcaaac tcacgccgga gtcgagtccc gtgtacgtcg acctcggcta
     1621 tgcctatgaa aacgacggca atctggacaa agccctggaa aactatctca aagcaatcga
     1681 attgaataag ggccagtatg cgactgcttt tctgcgggcg ggaattgtgt accagcgcaa
     1741 gggcgacaca gacaaagcga ccgcccggtt tgaccaggcg gagcgcttgt accaggcagc
     1801 cagtaacaac gaaggcctga acgaggtgta tcgccagcgc ggcattcttt ttcgtaacaa
     1861 cggcctttac gacgaagcga agaagcagtt tgagcagtcg ctcaacgcgg cacgttcgtt
     1921 gggcaacgag gcccaacaga tcacctcatt gattggtttg agttatctcc attcgtcgcg
     1981 tggtttgtac gcggaggccg aaaactatgc gcaacaggca gtgaccattg cgcagcagaa
     2041 aaacctggag aacctcgctg cgaatggtct gctcgaggtt ggtaatagct actcgaccaa
     2101 aggcgacctt ccgaaggcag aacagtattg gacccaggca attcagcttg ctcgggtgaa
     2161 taaaggaagg ctgacagaac atcgaggatt gacgaacctc ggcgggcttt acattcaaac
     2221 cttgcgcatc gacgaaggct tgcgcatggt gcagcaggcg tttgcgtatt tccaacagga
     2281 caactacccg cgtattgccg gccaatgcc
//
LOCUS       DASTEY010000662         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_592103, whole genome shotgun sequence.
ACCESSION   DASTEY010000662 DASTEY010000000
VERSION     DASTEY010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592103"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2386)
                     /locus_tag="VFO72_11520"
     CDS             complement(<1..>2386)
                     /locus_tag="VFO72_11520"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479969.1"
                     /translation="APVYSQNSAAQPKPADLIKALQYRSIGPYRGGRSAAVAGVPSQP
                     FVYYYGATGGGVWKTTDGGINWDSVSDGSVFGTGSVGAIAVADSDPNTIYVGMGESPI
                     RGNVSHGDGVYKSNDAGKTWKRVGLEDTRQIPRIRVHPKNPDLVYVAALGHVWGPNEQ
                     RGVFRSKDGGKTWEKILSRGNKAGAIDLILDPSNPNTIYAGFWEVYRKPWTLESGGPG
                     SGIFKSTDGGDTWTELTRNAGLPKGMIGKVGIAVSPANPERVWAIVEAEDGGVFRSDN
                     GGTTWTRINEDRRLRQRAWYYTRIYADPKSADTVYVLNVQFFRSNDGGRTYSTIQVPH
                     GDNHDLWIAPDDPNRMVQSNDGGANVSFNGGRSWTEQDQPTAQFYRVAIDNDFPYNIY
                     GAQQDNSTIRIASRTTGAGIGTSDWHDVGGGESGWIAPSPKDSNIVFAGSYGGLTTRY
                     DHRTGQERNISPYPNNPMGAGADVLKYRFQWNFPIVFSPHDPNTLYAAANVLFKSTDE
                     GSSWQIISPDLTRNDKSKQGASGGPITKDNTSVEYYATIFALMESPLQAGTIWAGSDD
                     GVVSLSRDGGKNWSNVTPPTSIMPEWIMINSLEASPHDPGTAYLAATMYKWDDNKPYL
                     YRTSDYGKTWKKITDGIPDTAFTRVIREDPNRRGLLYAGTETGMYVSFDNGERWQSLQ
                     LNLPVVPITDLAIQKREKELVVATQGRSFWILDELPLLHQLIDAGGFNSVSETRLFKP
                     KESYRMPGGGGFGGGSTATLGRNPANGVVVYYSLKAKPTADMTLEFLDPAGKSIR"
BASE COUNT          466 a          722 c          659 g          539 t
ORIGIN      
        1 ttcggatcga cttccctgcc ggatcgagaa attcaagcgt catgtcagca gttggcttcg
       61 ctttcaaaga ataataaacg acaacgccgt tcgcaggatt tcgaccgagc gtcgccgttg
      121 acccaccgcc aaaaccaccg ccacctggca tccgataact ctccttcggt ttgaagagac
      181 gtgtttcgct taccgaattg aaaccacccg catcgatcaa ctggtgcagc aacggaagct
      241 catcaagaat ccagaacgat cgcccttgag tcgcgactac cagttccttt tcacgtttct
      301 gaatcgccag atccgtgatc ggcacgaccg gcaggttgag ctgcaacgac tgccagcgct
      361 cgccgttatc aaacgacaca tacatgccgg tttcggttcc ggcgtagagc aagccgcgcc
      421 ggtttggatc ctcacggatg acgcgtgtga aagccgtgtc gggaatcccg tcggtgatct
      481 tcttccacgt cttgccatag tcgctggtgc ggtaaagata tggtttgttg tcgtcccact
      541 tgtacatcgt tgcagccagg taagccgtgc ccggatcgtg cggcgacgct tcgagtgagt
      601 tgatcatgat ccactccggc atgatgctgg ttggtggcgt gacgttggac cagtttttgc
      661 cgccatcgcg cgaaagagat accacaccat catcgcttcc ggcccagatc gttcccgcct
      721 gtaacggcga ttccatgagc gcgaagatcg tcgcgtagta ttcgacgctc gtgttgtcct
      781 tcgttattgg accaccggaa gcaccttgct tcgatttgtc gttgcgcgtc aggtccgggc
      841 tgatgatttg ccagctgctg ccctcgtcgg tcgacttgaa gagcacgtta gccgccgcgt
      901 atagcgtgtt tggatcgtgc ggtgaaaaca ctatcgggaa gttccactga aaccggtact
      961 tcagcacgtc cgcgccagcg cccatcggat tgttcggata cggactgatg ttgcgttcct
     1021 gtccggtgcg gtgatcgtag cgcgtagtca acccaccgta agaaccggca aaaacgatgt
     1081 tcgaatcttt gggcgagggc gcaatccagc ccgactcgcc gccgccgaca tcatgccaat
     1141 cactggtgcc gatgcccgct cccgtggtgc gactcgcgat gcgtatggtt gagttgtcct
     1201 gctgcgcgcc gtagatgttg taaggaaaat cgttgtcgat cgcgacgcga taaaactgcg
     1261 ccgtgggttg atcctgttcg gtccagctcc ggccaccgtt gaaagagacg ttcgcaccgc
     1321 cgtcattact ctggaccatg cgattcggat cgtccggagc gatccagaga tcgtgattgt
     1381 ctccgtgcgg tacctgaatc gtgctgtacg tgcggccgcc gtcattcgag cggaaaaact
     1441 gaacattcaa cacgtagacc gtatcggcgc ttttcggatc tgcgtagatg cgcgtgtagt
     1501 accacgcgcg ctggcgcagg cggcggtcct cgttgatcct ggtccacgtc gtgccgccgt
     1561 tgtcggaacg aaaaacgccg ccgtcttcgg cttccacgat cgcccacacg cgctcgggat
     1621 tcgcgggcga gactgcgatt ccgactttcc cgatcattcc cttcggcaag ccggcattgc
     1681 gcgtcagctc agtccacgtg tctccaccgt cagtcgactt gaagatgcca ctacctggac
     1741 caccactctc gagcgtccac ggcttgcgat agacttccca gaatccggcg tagatcgtgt
     1801 ttggatttga cggatcgaga atcagatcaa tcgcgcccgc tttgttgcca cgactcaaga
     1861 tcttttccca cgtcttgcct ccgtccttgg aacgaaagac accgcgttgc tcatttggtc
     1921 cccaaacatg gcccaatgcc gcaacgtaaa ccaggtcggg attcttcggg tgcacacgga
     1981 tgcgcgggat ctgtcgcgtg tcttcgagac cgactcgctt ccacgtcttg cctgcatcgt
     2041 tcgacttgta aacaccatcg ccgtgcgaga cgttgccgcg aatcggcgac tcacccatgc
     2101 cgacgtaaat cgtgtttgga tccgaatccg cgactgcgat ggcgccaacg gagcctgttc
     2161 caaacaccga gccatcgctg accgagtccc agttgatgcc accgtctgtc gttttccaaa
     2221 cgccaccgcc agtggcgccg tagtaataaa caaacggctg cgaaggaacg cccgcgaccg
     2281 ccgccgagcg accgccgcga tacggaccga tgctgcggta ctgtagcgct tttatcagat
     2341 cggccggttt tggctgtgcg gccgagtttt ggctgtaaac cggagc
//
LOCUS       DASTEY010000663         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_180007, whole genome shotgun sequence.
ACCESSION   DASTEY010000663 DASTEY010000000
VERSION     DASTEY010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180007"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VFO72_11525"
     CDS             complement(<1..1330)
                     /locus_tag="VFO72_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479970.1"
                     /translation="MAVATLALGIGANTAIFSVVNAVLLRPLPLKDPERLFVLWERRA
                     NSGRANLPVSGHEYAAFKEQTPSVEALTLIQPTGFNLTGRGDPVMVDALAVSTDYFEV
                     VGVPPLLGRTFLPGEDGAGGARVAVLGQKLWTQRFGADPGVLNQTVSLNDQSYTVVGV
                     MPALELMPEIIVPIDLAGEVRKVGKHSHQVIGRLKPAITLDQAQAEFAHTAKRLEEEF
                     AAANLGHGVQLVGLHEEVTGNAQLALLTLFGAVGFVLLIACANVANLLLSRAAARQKE
                     MAIRTALGAGRWRLIQQMLTESLLLAFLGGGIGLLAAFWLVNLMSRITAVDFPRLDQV
                     GVDTNVLMATIGFSVLTGFLTGLAPAWRNSEARLYQWISEGTRGSASLGRRRISSGLV
                     VVEVALAVVLLVGGGLMLKSFVRLLTVDPGFEPHQVLRLDLALAGSKYREP"
     gene            complement(1399..>3432)
                     /locus_tag="VFO72_11530"
     CDS             complement(1399..>3432)
                     /locus_tag="VFO72_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9479971.1"
                     /translation="GRAFLEEEDQPGANNVAIVSEGFWQRRYGRDESLVGRSLTLDDK
                     PYTVVGVMPSGFRFPGEYDIWLPLALDPVREFQGDWVSLVEVVGRLKPGTSRESARSE
                     LSLIAHSTSEKREGFFPVSVVEVAPLHQQLAGGVRLTVLVLWGAVGLVMLLACVNVAG
                     LMISRTLARQREMAVRAAVGARRWQLIRQLLTESVVLGFAGGVMGLLIAIWSIKAIGS
                     LAPRGFATSVYDLNNIPLDWRVFTFTLALSVVTGIVFGLAPALTASKPDLIQALRNSR
                     SAGLMSFGLRSFRGWLVVAELALAMVLLVAAGLLVRSFNKLTAVDVGFDRDHVLTAQI
                     SLPRSRYKQPEKTLAFQQELIERIKALPGVQSVGTINHTPLNGFGLIIFTEIEGNPPI
                     DRKEPPVGAGVVSPEYFQTLRIPLLTGRHVDDRDGPATQKVAVVNQAFAQRFFPNGDV
                     LGKRVGFVCEEAEGLCRTIVGVVGNIRQESLTDDVAAELYLPSAQLPVNSMTLFVRTA
                     SDPLSFVSAVRSQVQAVDKNQPIYDVKTLAQRVSDATAVSRSLTLLFGVFALLALVLG
                     SVGIYGIVSYAVTQRTQEIGIRMALGAKAGDILQLVLKHGLVLVLSGIVIGVAGALAL
                     TRFLSTLLFGIKPTDTLTFVIVSASFFLIAMFASLIPARRATKVDPLIALRYE"
BASE COUNT          819 a         1023 c          928 g          662 t
ORIGIN      
        1 gaggttctcg atacttcgaa cctgccagag ccagatcgag ccggagcact tgatgcggct
       61 caaatcccgg atcgactgtc agcagacgca caaaactctt cagcatcaag ccgccaccga
      121 ctagcaggac caccgccaaa gccacttcaa ctacaaccag gccactgctg atacgacggc
      181 ggccaagact cgccgaacct cgcgtgcctt cgctaatcca ctgatacagg cgcgcttcgg
      241 agttgcgcca tgcaggtgcg agtccggtta ggaatcccgt gagaacggaa aaaccgatgg
      301 tagccatcag tacattggtg tcgacgccga cctgatccag ccggggaaaa tcgaccgccg
      361 taatcctgct catcaggtta acgagccaga atgcagccag cagtccaatg cctccgccta
      421 agaaggcaag caacagactt tctgtcaaca tttgttgaat caagcgccag cgtcctgcgc
      481 cgagcgcggt ccggatcgcc atctctttct gccgcgccgc agcccggctc aagagcaggt
      541 ttgctacgtt ggcgcaggca atcaacaaaa caaacccgac tgctccaaac agtgtgagga
      601 gcgctaattg agcgtttccc gtgacctcct cgtgcagccc tacaagctga acaccatgcc
      661 ccaaattcgc ggctgcaaac tcctcttcaa gtcgcttcgc cgtgtgggca aactctgcct
      721 gtgcctggtc caaagtgata gcgggtttga gccggccaat cacctgatga ctatgcttgc
      781 cgactttgcg cacttcacca gcaagatcga tcggaacaat gatttccggc atcagttcta
      841 aagccggcat tacgccgacg accgtataac tctggtcgtt gaggctgaca gtttggttta
      901 acactcccgg atcagcaccg aaacgttggg tccaaagttt ctgtccgaga actgcaaccc
      961 gcgctccgcc ggcgccatcc tcacccggta aaaacgtccg gccgagcaac ggcggtacgc
     1021 caacaacttc gaagtaatcg gtcgaaacgg cgagtgcatc aaccatcacc ggatcacccc
     1081 tgccggtgag attgaacccc gtcggctgaa tgagtgttag cgcttcgaca gatggagttt
     1141 gttctttgaa ggcagcgtat tcgtgaccgg agaccggtaa atttgcgcgg cccgaatttg
     1201 cgcgccgttc ccaaagcacg aaaagtcgtt cgggatcttt caatggcagc ggccgcagca
     1261 gcaccgcatt cacgacgctg aagatggcgg tgtttgcgcc gatgcctaga gcaagcgttg
     1321 caacagccac agccgtgaaa ccagggcgct tgaataaact gcgaaacgca aattgaagat
     1381 ctttgaagat agagtccatt actcgtacct caatgcaatt aatggatcga ctttcgtcgc
     1441 gcggcgcgcc gggatcagcg acgcaaacat cgcgattaag aaaaaactgg ctgagactat
     1501 gacaaacgtc agcgtgtcag tcggcttgat gccgaagaga agagtggaca ggaagcgagt
     1561 caacgcgagt gcgcctgcga ctccgatgac gatgccgctc agaaccagta cgaggccgtg
     1621 cttgaggacc agttgcagta tgtctcccgc tttggcgcca agggccatgc ggattccgat
     1681 ctcctgcgtc cgctgcgtga ctgcgtacga gacaatgcca tagatgccca ctgagcccag
     1741 caccagcgcg agtaaagcaa agacaccaaa cagaagcgtt aacgagcgtg agacggcagt
     1801 cgcatcggaa acgcgttggg ccaacgtctt gacgtcatag atgggctggt tcttgtccac
     1861 tgcctgaact tggctgcgta ctgccgaaac aaatgagaga ggatcggaag cggtgcgcac
     1921 gaacaaagtc atgctgttta caggcaactg cgcactcggc agataaagct ccgcggcaac
     1981 atcgtcagtc aaactttcct gccggatgtt tccaacgacg ccgactatcg tgcggcataa
     2041 accttctgcc tcttcacaga caaaaccgac tcgtttcccc aacacgtcgc cattcggaaa
     2101 gaagcgttgg gcaaaagcct gattcacgac cgctactttc tgagtcgctg gtccatcacg
     2161 gtcgtctacg tggcggcctg tcaatagcgg aatgcgcaac gtttggaagt attcaggtga
     2221 aacgactcca gcgccgacgg gcggctcctt gcgatcgatt ggcggatttc cttcaatctc
     2281 ggtgaagata atcaggccga agccgttgag tggcgtgtga ttgatcgtgc ccactgactg
     2341 cacgcccggc agcgctttga tgcgttctat tagttcctgt tgaaaggcga gggtcttttc
     2401 aggttgttta tatcttgaac gcggcagact gatctgtgca gtcagtacat gatcgcgatc
     2461 aaagccgaca tctaccgcag tgagtttgtt gaaactacgc acgagtagtc cggcagcaac
     2521 tagcaggacc atcgcgagtg cgagttctgc gactacaagc cagccgcgaa aactgcgcaa
     2581 accgaaactc atcagtcccg cggaccggct gtttcgcagc gcctgaatca gatcgggttt
     2641 cgaagcggtc aacgccggcg ccagtccaaa cacgatcccc gtcacgaccg ataaagccag
     2701 agtgaaagtg aacacgcgcc agtcgagcgg gatgttgttc aagtcataaa ctgacgttgc
     2761 gaaacctcga ggcgcgagtg agccgatcgc tttgatggac cagatggcga ttaacaaccc
     2821 catcacaccg ccggcgaaac cgagcacgac actttccgta agcagttgcc gaatcagttg
     2881 ccaacgccgc gcgccgacag cagcgcgcac cgccatttcg cgttgccgcg ccagagtgcg
     2941 cgagatcatc aggccggcga cattcacgca cgcgagcagc atcaccaggc cgaccgcgcc
     3001 ccaaaggacg agcaccgtca gcctgacacc gccggcgagt tgttggtgca gcggcgccac
     3061 ctcgacgacg gaaaccggga aaaacccctc acgtttttcg gaagtgctgt gcgcaatgag
     3121 actcaactcg cttcgcgcac tctccctgct tgtacctggc ttgagccggc ccacgacttc
     3181 gactaatgaa acccagtcgc cttgaaactc cctcaccgga tcgagcgcca gcggcaacca
     3241 gatatcgtac tcacccggaa aacgaaaacc tgacggcatc acgccaacga ccgtgtaggg
     3301 tttgtcgtcg agtgtgaggc ttcggcccac gagtgactcg tcgcgtccgt agcgtcgttg
     3361 ccagaaaccc tcgctgacaa ttgcgacgtt gttcgcgcca ggttggtctt cttcttcgag
     3421 gaatgcgcgg cc
//
LOCUS       DASTEY010000664         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_575388, whole genome shotgun sequence.
ACCESSION   DASTEY010000664 DASTEY010000000
VERSION     DASTEY010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575388"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFO72_11535"
     CDS             <1..706
                     /locus_tag="VFO72_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479972.1"
                     /translation="ALEQYDNALKIYETIRNNFRIAFCKANRAKILARLGQFEEAKRL
                     LDELFASESKYLQLIPELHLVRAEMSLSQGDLAQATASANEAIKTGGEKSDVAIQAKF
                     LLGVVKATSGGRQEAQKLCTEAINAASNAGDFGLHSRALLAGAEAALKGNDAQTALTL
                     ATQAQARFARGEQLESEWRAWIFASRASQQLNDTVKAAEQLKTAQNTRYKLEQQWSPD
                     VFRQYAARPDIQAYYR"
     gene            757..897
                     /locus_tag="VFO72_11540"
     CDS             757..897
                     /locus_tag="VFO72_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479973.1"
                     /translation="MNPDNQPLEPIDPPDNTGGDSDAEADPVTTEAQPRKIDPPDNTG
                     GG"
     gene            complement(956..>2117)
                     /locus_tag="VFO72_11545"
     CDS             complement(956..>2117)
                     /locus_tag="VFO72_11545"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET9479974.1"
                     /translation="VLRQVELPLAMGVIVTGIRVATVIAVGVTTIAAAVGAGGLGVYI
                     FRGLRQYDNNLLLAGALSAALLALAADFFLGLLERQFSFEAKHRSVLPKVALAAAVLL
                     VVVGGIAIWRSGDNSKSSEQVRIGSKDFTESSLLAEIVAQMLEARGVSVERRFELGGN
                     LPHEALVSGTLDLYPEYTGTSYTAILRHAPTGDPRAVYDQVKQEYASKFNVEVSPPLG
                     FENTFAILVRGEEARRLNLKTISEAAPHTASWRAGFGQDFMSRADGYPGFSKTYGLKF
                     AEVREMDLSLTYIALSSRQVDLIAGNSTEGRIATLDLFQLADDRRYFPPYEAVYLVRQ
                     DSLTRVPALREVLSKLANAISTEEMRRLNYEIDGNKRDPKEVVREWLSGRGF"
BASE COUNT          527 a          613 c          569 g          408 t
ORIGIN      
        1 agcgctggag caatacgaca acgcgctcaa gatttacgaa acgatccgaa acaattttcg
       61 catagctttc tgcaaggcta accgtgcaaa gattttggcg cgactgggcc agtttgaaga
      121 agcaaagcgg ttgctcgacg aactcttcgc gagtgagtca aagtacctgc aattgatacc
      181 ggagcttcat ctcgttcgtg ctgagatgag tctcagtcaa ggcgacctgg cccaggctac
      241 ggcgtccgcg aatgaggcca tcaaaacggg cggcgagaaa tcggacgtgg cgatccaggc
      301 aaagtttctc ctgggcgtgg tgaaagccac ttcgggagga cgtcaggaag cgcaaaaact
      361 ttgcaccgag gcaatcaacg ccgcgtcaaa tgcaggcgat ttcggcctac actcccgcgc
      421 gctgttggct ggcgctgagg ctgccttgaa aggaaatgac gcccagaccg cattaacatt
      481 ggcgactcaa gcccaggcca ggttcgcacg aggtgaacag ctcgagtcgg aatggcgtgc
      541 atggattttt gcctcacgtg ccagccagca gttgaatgac acggtcaaag cggcagagca
      601 actaaaaacc gcgcagaata cgcgttacaa gttagaacaa cagtggagcc cagatgtttt
      661 taggcaatat gccgcaaggc ctgacattca ggcttactat cgttaacaaa gttgatccaa
      721 ctggttcaac tcccaaaact agaaggagat cagacaatga acccagataa ccaacctctc
      781 gaacctattg atccaccaga caacactggt ggtgacagcg acgccgaagc ggatccggtt
      841 actactgagg cccagccaag gaagatcgat ccaccggata acaccggcgg cggttagatg
      901 aaaaatagcc acggataaca cgaaaaatcc ggatttagtc cgtgttatcc gtggctcaaa
      961 accctcgccc actcagccac tctcgtacaa cttcctttgg atcacgtttg ttgccatcaa
     1021 tctcataatt gagacggcgc atctcttcag tcgaaatcgc attcgccaac ttcgacaata
     1081 cttcccggag cgcgggaacg cgtgttaacg aatcctgccg caccaggtaa accgcttcgt
     1141 acggaggaaa gtagcggcgg tcgtcggcga gctgaaatag atcgagcgtg gcgatgcggc
     1201 cttcagtgga gttgccggcg atcaggtcga cctgacgtga cgagagcgcg atgtaagtca
     1261 acgacaaatc catctcccgc acttccgcga acttcaatcc atacgtcttt gaaaaacccg
     1321 gataaccatc ggcgcgcgac atgaagtcct ggccgaaccc ggcacgccag ctcgctgtgt
     1381 gcggagcagc ttcagagatc gttttcaggt tgagccgtct ggcttcctcg ccgcgcacga
     1441 ggatcgcgaa cgtgttttcg aaaccgagcg gcgggctgac ctcgacgttg aacttgctcg
     1501 catattcctg tttcacctga tcgtaaaccg cacgcggatc gccggtggga gcgtggcgaa
     1561 gaatggccgt gtaggaagtt ccggtgtatt ccggatagag atcgagcgtg ccgctcacca
     1621 gcgcttcgtg tggaaggttt ccgcccagtt cgaaacgtcg ctcgacggaa acgccacgcg
     1681 cttcgagcat ctgcgcaacg atctccgcca gcagcgatga ttccgtgaag tctttcgatc
     1741 cgattcttac ctgctccgat gactttgaat tgtcgccact gcgccaaatc gcgatgccac
     1801 caacaactac cagcaacact gccgcggcga gtgccacttt cggcaacacc gatctgtgct
     1861 tcgcctcgaa actgaattga cgttccagta gtcctaaaaa gaaatccgca gccaaagcca
     1921 acagcgcggc cgacaacgcg cccgcgagca gtaagttgtt gtcgtactgc cgcaagccgc
     1981 gaaagatgta gacgccgagg ccaccagcac caaccgccgc ggcgatcgta gtcacaccta
     2041 ccgcgatcac cgtcgcgacg cggatgcccg tgacgattac gcccatcgcg agcggcagct
     2101 cgacttgtcg cagcacc
//
LOCUS       DASTEY010000665         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_410606, whole genome shotgun sequence.
ACCESSION   DASTEY010000665 DASTEY010000000
VERSION     DASTEY010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410606"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFO72_11550"
     CDS             complement(<1..385)
                     /locus_tag="VFO72_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET9479975.1"
                     /translation="MSIRTWLLVFCAFVFGGLFLTFVNSQRAQTGEPQPQASPSPSPS
                     PSPSPSPTPITGLHQWGAVTLFHGLPSDRVRAIAQTSDGAMWFGTEAGLAKFDGRRTQ
                     TINDPALPPGRVLALQVDQNGAMWVG"
     gene            complement(423..1205)
                     /locus_tag="VFO72_11555"
     CDS             complement(423..1205)
                     /locus_tag="VFO72_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9479976.1"
                     /translation="MSILIAEDNMAQRHYLREILEKEFPTHVPVFEAADGEETVKLAL
                     REKPEVCILDIQMPKLSGVKAARAIWRDFPTARIIFWTQFPHEIYINEIRKIVKSVDP
                     PPAYGFIHKNNPESRFLRFVAAVLEDGADMIDPAFKDSFKRPLLTEFEAEALYYLALG
                     LSNWAIARKCSLSLRGVESRLATLYEKLFISVAEGTEQEAYEKLAYNIRTRAFFEALR
                     RGLLNSDELEKAANDLEGWLQKDQKRFAEEQKREAASKKKSS"
     gene            1306..>2261
                     /locus_tag="VFO72_11560"
     CDS             1306..>2261
                     /locus_tag="VFO72_11560"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET9479977.1"
                     /translation="MTTQILLRPVAVCLMLVLIAGAGGLSAAAQTPEPRDERLWQRAL
                     AIHRRAIVVDTHNDVTTPMTNNHFDLGGTPPAPYRTNIARMKEGGLTAEFFSLYIRPW
                     YVQHGGSTRRTLDMIDAVYRAVERHPRDLMLATSVADIRRAKRLNKIAALMGIEGGHA
                     IEDSLAALREFYRLGVRYMTLTWNNTNNWADAGRGEKKHGGLSDFGREVVREMNRLGM
                     LVDVSHVSDETMSDALDVSKAPIIASHSSARALSDVSRNIPDDLLKRIAKNGGVVCVN
                     FYSAFVDVKTVGPQSAERDKRLKAQQDALVEKYKNDPERLAE"
BASE COUNT          503 a          654 c          632 g          472 t
ORIGIN      
        1 tgcctaccca catcgcgccg ttctgatcca cttgcaacgc aagcacccgt cccggcggca
       61 acgccggatc gttgatcgtt tgcgttctgc ggccgtcgaa tttcgcgagc cctgcttcag
      121 ttccaaacca catcgcgcca tcgctcgttt gcgcgatcgc acgcacgcga tccgacggca
      181 gcccgtgaaa caaggtgact gcgccccact ggtgcaggcc agtgattggc gttggtgacg
      241 gcgaaggcga tggagacggt gacggcgaag gtgatgcttg tggctgcggc tcgccggttt
      301 gggcccgttg actgttaacg aaagtaagga acagaccgcc aaagacaaac gcgcagaaaa
      361 ccaacaacca tgttctgatg ctcaatcgca atgggggtct accaggctca aagtacgaac
      421 aactaactac tcttcttttt cgacgccgct tcgcgttttt gttcttcggc aaaacgcttc
      481 tgatctttct gtagccagcc ttccagatcg tttgcggcct tctcgagttc gtcgctgttg
      541 agcagtccac ggcgcagtgc ttcaaaaaac gcgcgtgtgc gaatgttgta ggcgagtttt
      601 tcgtacgcct cttgctcggt gccctcggcg acgcttatga acagtttctc gtaaagcgtc
      661 gccaaccggc tttcgacacc gcgtaaactc agcgaacact ttcgcgcgat ggcccagttt
      721 gaaagtccga gcgcgagata gtagagcgct tcggcttcaa actcagtgag caacggtcgc
      781 ttgaacgagt ctttgaaggc ggggtcgatc atatcggcgc cgtcttcaag taccgctgcc
      841 acgaagcgca agaagcgcga ctccggattg tttttgtgaa tgaaaccgta agccggcggt
      901 ggatcgactg actttacaat cttgcggatc tcgttgatgt agatctcgtg cggaaactgc
      961 gtccaaaaaa tgatgcgcgc cgtcggaaag tctcgccaaa tagcccgcgc cgccttcacg
     1021 cccgacagct tcggcatttg gatatcgaga atgcagactt cgggcttctc acgaagcgca
     1081 agcttcacag tctcttcgcc gtccgcagcc tcgaacacgg gcacgtgcgt cggaaattcc
     1141 ttctcgagga tctcgcgcag gtaatggcgc tgggccatgt tatcttcggc aatcaggatt
     1201 gacatgacat ggtcgataat aatgctctgg acctccggta acaacgtggg tcgccacatt
     1261 ttgccgccgt gatatcattg cttgtcccaa gagaaaggtg aatccatgac aactcaaatc
     1321 ctgcttcggc cggtagccgt ctgtctaatg ctggttttaa tcgccggcgc cggcggtttg
     1381 tcagctgccg ctcaaacgcc ggaaccgcga gacgaacgac tctggcaacg cgcattggca
     1441 attcaccgcc gagccatcgt tgtcgacacg cacaacgacg tgacgacgcc gatgaccaac
     1501 aaccacttcg atctcggcgg cacaccgccc gctccctatc gcaccaacat cgcgcgcatg
     1561 aaggagggcg gtctgacggc ggagttcttt tcgctctaca tcagaccgtg gtacgtccaa
     1621 cacggcggct ccacgcgtcg cacgctcgac atgatcgacg cggtctaccg cgcagtcgaa
     1681 cggcacccac gcgatctgat gcttgccacg tcagttgccg acattcgccg agccaaacgt
     1741 ctcaataaaa tcgcggcgtt gatgggaatc gaaggcggcc acgcgataga agactctcta
     1801 gcggcgttgc gcgagttcta ccggcttggc gttcgttaca tgaccctgac gtggaacaac
     1861 acaaacaact gggccgacgc cggcagaggt gaaaagaagc acggcggatt aagtgacttt
     1921 ggcagagaag tcgtgcgtga gatgaaccgg ctcggaatgc tggtcgacgt gtcgcacgtt
     1981 tcggacgaaa cgatgagcga cgcacttgat gtttcaaaag cgccgatcat cgcttctcac
     2041 tcctcggcgc gcgcgcttag cgacgtctcg cgaaacattc ccgacgatct gttgaaacgg
     2101 atcgcgaaaa acggcggcgt agtatgtgtc aacttttaca gtgcgtttgt cgatgtaaag
     2161 acggttggac cacagagcgc cgaacgcgac aagcggttga aggcgcagca ggacgcactc
     2221 gtcgaaaagt acaaaaacga tccggagcga ctggccgaag a
//
LOCUS       DASTEY010000666         5841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_344910, whole genome shotgun sequence.
ACCESSION   DASTEY010000666 DASTEY010000000
VERSION     DASTEY010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344910"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3309)
                     /locus_tag="VFO72_11565"
     CDS             complement(<1..3309)
                     /locus_tag="VFO72_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9479978.1"
                     /translation="MTTSTSDRGRVLIVDDDRRILDLLVELLELEGYEVAAAEDGAEA
                     LELAQSFDPDVVVSDVVMPVVGGLELCRRLKEDQHTAYVPVLLISGIRKSDDAGMEGL
                     YAGADDYLDLPFRNEELLVKVARLVERHRIERHYREIVEQAADIIYTLNMSGYITSMN
                     AAGARFFGCTPIEMRGRHWSELVGSESATADIEQMKNHRGSSPLRSTYRLPDAKGELR
                     CLEGMSTVECDRRGQPIGVRGVVRDITEQQRADEAIRESEARYRELFENANDIIYTHD
                     LQGNFTSLNRNGERITGYSREETLRMNVADVIAPEYLGLAREMIAHKAAEHVSTVYEI
                     DIISKKGRRVRLEVSTRLILRDGKPVGVQGIARDLTERKHSEEALRETQAFFNSFMDN
                     SPAVAFMKDDTGRYVYVNKPFERLFGQKFSFLKGRMSFDWLPPATAAQTHEHDLKILN
                     SGEPEQIIETIPSPDGTPHYWLVFKFPTIDASGNKYVAGVGVDITERRRAEEALAQQA
                     QREAMIHRISQAIRCSLDSHEIYKTAVRELGTHLAVDRCSLFMKDDRAKCALNVAEYH
                     TEGVEPAASDFALDHLQSLIKALDETGVLPFTDAANDKRIADLYQNILSKADVKSIMY
                     VAIRVGDDVPAAFALSTTKQLRHWTDADIAMAKAVADQTGIAIRQAQLYQKAESTSIR
                     EALVNRLTTAIRASLRMPEVLSTATRELGRALGASRVHLFLYNSEEFDSPFEHEYLGP
                     DVMSIRPMPIGPDDPTAQRLSSSSEPLIIVDALNYDDESIVLRDYVREHAQRLNIRSQ
                     INYPLIVKGQFRGVLSIHQTDRIRRWTEDEVSLVEALAERLSIGIAQAELFEMVARGK
                     TEWETTFDAMSDGIFIFDRAGELKRVNRAGAAMEAVHPRLLLGRQCCDILRTSEEDDT
                     CVVENALETGRSITIEITPARLNRPLLVTIEPVFDESSKALAVVCTARDLSELRKVQA
                     VAREHQSLLNNILESARESIYAVDTDGNFKWCNTATLKGLGFQRSEFIGRPLLEMVYD
                     DDHELVREKLAAALTGLPQTYEMRYFAYDGHLRYARVDNSPLIVEGKTTGVLGIARDI
                     TEQKEERERAARADK"
     gene            complement(3949..5070)
                     /locus_tag="VFO72_11570"
     CDS             complement(3949..5070)
                     /locus_tag="VFO72_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479979.1"
                     /translation="MSRPLLLLLVIAVLMCGGVVLSAKSEASVSGAGPVAFASPSPTP
                     RTREELPQDSDEVVKFDTNLTNIFFTAADKNKRFISDLKAEDIRVLEDGQPQEIFTFQ
                     TNIDLPLSLAILIDTSASEERTLPDEKAAARAFLESVLRPNRDEAAIVSFTGETTLEQ
                     GFTGNVERLRRAIDRVEFVPPSGYIGGGVVVNGTPPISGTNQILAGSTAIWDAVWATA
                     EELISASAEHTRRAIILLTDGDDTSSRMKIHDAIERAQKADALVYAIGIGDRYTFNVD
                     EGALRKIAEKTGGRAYFPKHERDLRDAFAQIQRDLREQYLVAYSPSNKARDGSFRKIE
                     IQLVNPALRQQDLKLNYRSGYFAKSAAPEPPTRKRGQER"
     gene            complement(5100..>5841)
                     /locus_tag="VFO72_11575"
     CDS             complement(5100..>5841)
                     /locus_tag="VFO72_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9479980.1"
                     /translation="SDDLSLTTPLYVAVLMDTSPSTAGKLKFQQESAMNFIQTVVKPR
                     KDRVLFGTFDDEITLLQDFTDRLDLLDKAVYSVKKMGKQTALFDAVWQFCDEKMRSVP
                     GRRVLLLVTDGEDTYSRANIRDAIDIAQRTETTIFAISTKAGFLATVPGVEAGQVADK
                     KDRDLLTLAEETGGFAFFTGDMLSLERSFSKISKELRAQYLVTYDPTNKSYDGTFRKI
                     DVRLTNGHDKLRVRTKRGYKAIADSVMP"
BASE COUNT         1192 a         1626 c         1663 g         1360 t
ORIGIN      
        1 cttgtcagct cgcgcagcac gttcgcgttc ttctttctgt tcggtgatat cgcgcgcgat
       61 gccaagcacg ccggttgttt tgccttcaac gatcagagga gaattgtcga cgcgcgcgta
      121 tcgcagatgg ccatcgtacg cgaagtagcg catctcgtac gtttgcggca gtcccgtcaa
      181 cgcggccgcg agtttctcgc gtacgagttc atggtcgtca tcatagacca tttcgagcaa
      241 aggacggccg atgaactcgc tacgttgaaa acccaaacct ttcaacgtcg cggtgttgca
      301 ccatttgaag tttccatccg tatcgaccgc atagattgat tcgcgggcac tctcgagaat
      361 gttgttcagc aacgactggt gttcgcgagc aacagcctgc accttgcgca gttccgaaag
      421 gtcgcgtgcc gtgcacacca cggccaatgc cttgctagat tcgtcaaaca ccggttcgat
      481 ggtcaccagc aacggacgat taagacgcgc gggcgtaatt tcgatcgtga tgctgcggcc
      541 agtctccagc gcgttttcaa ccacgcacgt atcgtcttct tcgctagtgc gcaagatgtc
      601 gcaacattga cgccccagca gtagtcgtgg atggaccgct tccatcgctg cgcccgcgcg
      661 gttcacgcgt ttgagttcgc cggcgcggtc aaagatgaaa atgccgtctg acatcgcgtc
      721 aaacgtcgtt tcccactccg tcttgccacg ggccaccatc tcgaacaatt cggcctgcgc
      781 gataccgatc gacaatcgtt cagccaaagc ttcgaccagc gacacttcat cttcggtcca
      841 acgccggatg cgatccgttt ggtgaatact caacacgccg cgaaattgac ctttaactat
      901 cagcgggtag ttgatctggg agcgaatgtt gagtctctgc gcatgctcgc ggacgtaatc
      961 acgcaaaact atcgactcgt cgtcatagtt cagcgcatcg acgatgatta aaggctcact
     1021 tgagctggaa agccgctggg cagtcggatc gtctggacca attggcatcg gcctgatgct
     1081 catcacgtca ggtccgagat attcgtgttc aaatggtgaa tcgaattcct cgctattgta
     1141 aagaaacagg tgaactcgcg acgcaccgag cgcgcggccg agctcgcgag tagctgtgct
     1201 aagcacttcc ggcattctca acgaagcgcg aatcgccgtg gtcaaacgat tcacgagcgc
     1261 ctcgcgtatc gaagtgctct cagccttctg gtaaagctgc gcctggcgga tggcaatgcc
     1321 ggtttgatcc gcgaccgcct tggccatggc gatatccgcg tccgtccaat gccggagctg
     1381 cttcgttgtc gataaggcaa aagcagccgg aacgtcgtcg ccgactctga ttgccacgta
     1441 catgatcgac ttcacgtcgg cttttgagag gatgttctgg tagaggtcgg cgatgcgttt
     1501 gtcgttcgcg gcgtctgtga aaggcaggac accagtttcg tccaacgctt tgataagcga
     1561 ttgcagatgg tccagagcga agtctgacgc tgcgggctcc accccttctg tgtgatactc
     1621 cgcgacgttc aacgcacact ttgcgcgatc gtctttcatg aacaacgaac aacgatcaac
     1681 cgcgagatga gtaccaagct ctcgcaccgc cgtcttgtag atttcgtgac tgtcgagcga
     1741 acaacggatc gcctgcgaga tgcggtgaat cattgcctcg cgttgagctt gttgcgccag
     1801 cgcttcttcg gcgcgacggc gttctgtgat gtcgacaccg acacccgcta cgtatttgtt
     1861 acccgaagcg tcgatcgtcg gaaacttgaa gaccagccag taatgcggcg tgccgtcagg
     1921 gctcggaatc gtctcaataa tctgttcagg ctcgccgctg ttgagaatct tgagatcgtg
     1981 ctcgtgcgtt tgcgcggcag tggcgggagg cagccagtcg aaactcatgc ggcccttgag
     2041 aaaactgaac ttctggccga agagacgttc gaaaggtttg ttgacgtaga catagcgtcc
     2101 cgtgtcgtct ttcataaacg cgaccgcggg actgttgtcc atgaaactat tgaagaacgc
     2161 ctgtgtctcg cgcaacgctt cttctgagtg ctttcgctcg gtgagatcac gagcgatgcc
     2221 ctgcacacca acaggcttgc cgtcgcggag gatcaaacgt gtgctgactt ccagccgcac
     2281 acgacgcccc tttttcgaaa tgatgtcgat ctcgtaaaca gtcgaaacgt gctcggccgc
     2341 tttatgtgcg atcatttcgc gcgcaagtcc gagatactca ggcgcgatca cgtcggcgac
     2401 gttcatcctg agtgtttctt cacgcgagta acccgtgatt cgttcaccat tgcgattcaa
     2461 tgacgtgaag ttgccctgaa gatcgtgcgt gtagatgatg tcgttggcgt tttcaaacaa
     2521 ctcgcgataa cgcgcttcac tttctcgaat agcttcatcg gctcgttgct gctcggtgat
     2581 gtcgcgcaca acaccgcgaa caccgattgg ctgaccacgt cgatcgcact cgaccgtgct
     2641 cattccttcg aggcatcgca gttctccctt cgcatcgggc aaacgatacg tcgagcggag
     2701 tggcgacgag ccgcgatggt ttttcatctg ctcgatgtcg gccgtcgccg attccgaacc
     2761 gacgagttca ctccagtggc ggcctcgcat ctctattggc gtgcaaccaa aaaaacgtgc
     2821 gccggccgcg ttcatgctgg tgatgtaacc actcatgttg agggtgtaga tgatgtcggc
     2881 ggcctgctcg acgatctcgc ggtagtggcg ctcgatgcga tggcgctcaa ccagccgcgc
     2941 gaccttcacg agtagttctt cgttacgaaa cgggagatcg agatagtcgt ccgcgccggc
     3001 gtagagaccc tccattcccg cgtcgtctga ttttctaatg ccgctgatca gaagcaccgg
     3061 aacgtatgcg gtgtgctggt cttccttcaa acggcggcaa agctcgagtc cgccgacaac
     3121 cggcatcacg acatcgctga caaccacgtc tggatcgaac gactgcgcga gttccagagc
     3181 ttcagcgccg tcctcggcgg ccgcgacttc ataaccctcc agttcgagga gctcgaccag
     3241 cagatcgaga atcctccgat cgtcatcgac aatcagtact cggcctcggt ctgaggtgct
     3301 ggtggtcatt tagtctgtta tccgaacgac tttgagaatt accccgggga ggtgcgttgg
     3361 agtggggttc tggcgccagg cggacacact ggcggacagg agtataacag gtttaataaa
     3421 agcttgtcac tgacttggct taagatttct aactgctaaa cagctgtaaa tcctgctacg
     3481 acctgggata gtgaagcgta gtgggtcagt ttgcatttaa cgccgaaggc gttagctaat
     3541 tccagcccag aggttggagc ttgcgacaac cctggggact gattggatgt ttttaccttt
     3601 tacgctgaaa gcgttggcta accgcgaatt tgccaacgcc ttcagcgtta ggacgggacg
     3661 ctaagtttcg acccagggtt gaagcaaccc tgggctggaa attagcgaac gcctctggcg
     3721 ttccgattag gacactactg gactgaagcg tagtgggtca gtttgcattt aacgccttcg
     3781 gcgttcagat taggacccac cggatagtga tggtttgaaa attgaggcgc cttccttcct
     3841 gggaccgcag gcgtctcgcc tgcacggtga cgaggcgagc ggctgtcgtg ggctgcattc
     3901 cagacgcctg cggtcccagg taagaggatt gtagatttta agaggttttt accgctcctg
     3961 accgcgcttt cgcgtgggcg gttccggcgc cgcgcttttt gcgaagtagc cggaccggta
     4021 gttcagtttg aggtcctgct ggcgcaacgc gggattgacc aactgaattt caattttgcg
     4081 gaaggagccg tcgcgggcct tgttactcgg ggaataggcc accaggtact gctcgcgaag
     4141 gtcgcgctga atctgtgcga aagcatcgcg caggtctcgt tcgtgcttcg ggaaataagc
     4201 gcggccgccg gtcttttcag cgatctttcg caatgcaccc tcgtcgacat tgaacgtgta
     4261 gcggtcgccg ataccgatcg cgtagacgag cgcgtcggct ttttgcgctc gttcgatagc
     4321 gtcgtgaatc ttcatgcgac tgctggtgtc gtcgccgtcg gtgagaagga tgattgcgcg
     4381 gcgcgtgtgc tcggcggatg cgctaatcaa ctcttcagca gtggcccaaa cggcgtccca
     4441 aattgccgtc gatccggcga gaatttgatt cgtgccggag attggcggag tgccgttcac
     4501 gaccacgcca ccgccgatgt aaccggaagg tggcacaaac tcaacgcgat cgatcgcccg
     4561 gcgcaggcgc tcgacattgc cggtgaaacc ctgttcgagt gtcgtctcgc cggtgaaaga
     4621 aactatcgca gcctcgtctc tgtttggccg caacacgctt tcgagaaacg cgcgggcggc
     4681 ggctttctcg tcgggaagcg ttcgttcttc ggaggcgctg gtgtcgatca gaatcgcgag
     4741 cgagagcggc agatcgatgt ttgtctggaa cgtgaagatc tcctgaggct gtccgtcttc
     4801 gagcacgcgg atatcttccg ccttcagatc gctgatgaaa cgtttgttct tgtcagcagc
     4861 agtgaagaag atgttggtga gattggtatc gaacttgacg acctcgtcgg agtcctgcgg
     4921 caactcttca cgagttcgcg gagtcggcga tggcgatgca aatgcgactg gtccggctcc
     4981 cgaaacactg gcttcgctct tggcggacaa aactacgcct ccgcacatca gcacggcgat
     5041 aaccagcaac aacagaaggg gacgactcaa ttctcggtaa cgcatcttgc ctctaaactc
     5101 tacggcatca cgctgtcggc gatcgctttg tagccgcgtt tcgttctgac tcttaatttg
     5161 tcatgcccgt tcgtcagtct tacgtcgatc tttcggaacg tgccgtcgta actcttgttc
     5221 gtcgggtcat acgtgacaag gtactgcgcg cgcagttctt tcgagatctt tgaaaacgag
     5281 cgctcgaggg agagcatgtc tccggtaaaa aaggcgaagc cgccggtctc ttccgcgagc
     5341 gtcagcaagt cgcgatcttt cttatcggca acttgtccgg cttcaacgcc gggaacggtt
     5401 gcgagaaacc ccgctttggt tgaaatcgca aagatggtcg tttcggttcg ctgcgcgatg
     5461 tcgatggcgt cgcgaatgtt ggcgcggctg taggtgtctt caccgtcggt gaccagcagc
     5521 aacacacgcc gcccaggaac tgaacgcatc ttttcgtcgc agaactgcca gacggcgtcg
     5581 aaaagcgcag tctgcttgcc catcttcttc acactgtaga cggccttatc gagcaggtcg
     5641 agcctgtcag taaaatcctg aagcaacgtg atctcgtcgt cgaaggtgcc gaagagaacc
     5701 cgatctttgc gcggtttcac cacagtctgg atgaagttca tcgccgactc ctgctgaaac
     5761 ttcagcttgc cggcggtcga aggcgatgta tccatcagca ccgcgacgta gagcggtgtg
     5821 gtgagcgaga gatcgtctga a
//
LOCUS       DASTEY010000667         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_740245, whole genome shotgun sequence.
ACCESSION   DASTEY010000667 DASTEY010000000
VERSION     DASTEY010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740245"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFO72_11580"
     CDS             <1..318
                     /locus_tag="VFO72_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479981.1"
                     /translation="LEDRKTETKDGKTEEMLHARHILFSEASSNPFGPPQTAREKARS
                     AVEQEKAKKILDDIVARSHVKVADNYNVKAPEQQPMQGLPPGFGAPPSEAPAQEAKPS
                     PKK"
     gene            353..1213
                     /locus_tag="VFO72_11585"
     CDS             353..1213
                     /locus_tag="VFO72_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9479982.1"
                     /translation="MQLGDYAVQVIQDAEFRLDGGAMFGVVPRKLWSQVCPPDDENRI
                     RMNMNCVFVDTGSEKVLIETGIGDKWPAKQSAMYGIHRARALTDTLFEQTGVSADDVT
                     IVVNTHLHFDHAGGNTRLDSDGNAVALFPNARYFVSRAELVHAEEPTERDRASYLPEN
                     WRPLVASGQLEPKDAKYEVVPGLTMETIAGHNRSMQCWRLTRDGKTMFGFADLVPTRA
                     HLRLPWIMGYDLYPVETLAAKKRLLPEAARNNWLCLFYHDPDEPVCRLREGEKEFVPV
                     PYRGEEKNGS"
     gene            1210..2091
                     /locus_tag="VFO72_11590"
     CDS             1210..2091
                     /locus_tag="VFO72_11590"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET9479983.1"
                     /translation="MSNRPRVRFGIIGGSGLYQMPELTAVEEVKVPTPFGEPSDSFVI
                     GTLEGERVAFLPRHGVGHRYTPTEVPFRANIYAMKLLGVERILSASAVGSLQEKYAPL
                     DMVIPDQFFDRTRGRVPESTFFGDGIVAHVSFAHPVCSALGDVLEESCRVADVKTHRG
                     GTYLCMEGPAFSTVAESNVYRSWGMDIIGMTNLQEAKLAREAEICYATLALVTDYDCW
                     HPGHDAVSVETVVEYLNKNVRNAQIILREAVKRLKNSERTCKCGSALQHAIFTQPSLW
                     PAETAKKLEAIVRKYQG"
     gene            2231..2716
                     /gene="bamD"
                     /locus_tag="VFO72_11595"
     CDS             2231..2716
                     /gene="bamD"
                     /locus_tag="VFO72_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET9479984.1"
                     /translation="MKGSFVLMLIFAAVLLVPAPAAQAQTVRTGPDPNTLRDPDLEKD
                     SQHNLEVAKNYFKLKKAYVAALKRCEEIIAGNPNFAKIEEVLLMAGQSSLWLSEKKGK
                     QSPDLYVTFDGGERRTLTSEQFREMGRESLSKLIKDYPDGSYTKQAREELRARSLEAK
                     P"
     gene            2720..3643
                     /locus_tag="VFO72_11600"
     CDS             2720..3643
                     /locus_tag="VFO72_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9479985.1"
                     /translation="MSLLVVGSVAYDAVETPFGKCEKMLGGSASHFSISASFFTDVSV
                     VAVVGGDFTDFDIFEKHRIGTRDLECIDDGKTFRWGGRYDYDLNVAHTLFTDLNVFAD
                     FQPKLSEESKRSRLAFLGNIQPDLQRGVREQLPDAELVALDTMNLWIEHTRESLLRTI
                     RIVDVVIINDAEARQLTGIPNLIKAAREILSWGPKALIIKRGEYGAALFTADSYFAIP
                     AYPLEAVFDPTGAGDTFAGGFMGYLASQERLDEDAMRRAMIFGSVMASFNVEEFGTER
                     VRRLSHAEINDRFRAFKRMTHFDEIPFVARV"
     gene            3735..4118
                     /locus_tag="VFO72_11605"
     CDS             3735..4118
                     /locus_tag="VFO72_11605"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET9479986.1"
                     /translation="MFCPTCESEYEPGITRCPDDETLLVERLTPENTLHDNSEARFVS
                     FYKLGSPAEAEMVNDIFQKNDIRSVIKSGGADAFSPLLSATSEGAEIFVDERDVDRAA
                     EIYNAFFGADTTPLTGGPLEEDEEQ"
     gene            4121..4447
                     /gene="cutA"
                     /locus_tag="VFO72_11610"
     CDS             4121..4447
                     /gene="cutA"
                     /locus_tag="VFO72_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET9479987.1"
                     /translation="MPDKDAIVVFMTAANGEEATRLAELLIGAHLAACVQILPEMESV
                     YRWRGKIERQSEILLIVKTTTDKFADLEREVRALHSYDTPEIIAVPIVAGSTSYLEWL
                     DDSLKP"
     gene            complement(4444..5202)
                     /locus_tag="VFO72_11615"
     CDS             complement(4444..5202)
                     /locus_tag="VFO72_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET9479988.1"
                     /translation="MEDESNEIDRAPTGSTQDEQLLESPRPDEFTHTDPWRVFRIMGE
                     FVEGFDELAALTRGISIFGSARSKPGDPDYQAALETAALLAAEGYAVITGGGPGIMEA
                     ANRGAFEAGGLSIGCNIELPFEQRPNAYQTRSLKFKYFFVRKMMFVKYSLGFVIFPGG
                     FGTFDELFEALTLIQTKKIRNFPVVLFGSSYWSGLLDWLRQVVLTDGKISSHDLRMFH
                     VTDSPKDVLEFLNRSQSSLAELDKLVSDDIRIPS"
     gene            complement(5211..>5662)
                     /locus_tag="VFO72_11620"
     CDS             complement(5211..>5662)
                     /locus_tag="VFO72_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9479989.1"
                     /translation="TSREAFLTALEISAYSLAQLARAAAPLMTEGGSIVTMTYYGAEK
                     VVAGYNVMGVAKAALEASTRYLAKDLGPRKIRINAISAGPIQTLSARGVADFSTMLKH
                     HAERAPLGRNVEPREVGNTAVFLCSSMASGITGEVIYVDCGYNIMGI"
BASE COUNT         1251 a         1487 c         1690 g         1234 t
ORIGIN      
        1 cttgaagacc gaaagaccga aacgaaggac ggcaagacgg aagagatgct gcatgcgcgg
       61 cacattctgt tcagcgaagc gagcagtaat ccgtttggtc caccacagac tgcgcgcgag
      121 aaagccaggt ctgcggtcga acaggaaaag gccaagaaga ttctcgacga tattgtcgct
      181 cggtcacacg tgaaagtggc agataactac aacgtgaagg cgcctgagca gcagccgatg
      241 cagggactgc ctccgggctt tggcgcacct ccaagcgagg cgccggctca ggaagctaaa
      301 ccgtctccta agaagtgaat ctgtgtaatc tgtggctggc cccggatgaa tcatgcaact
      361 cggcgattac gcggtgcagg tgattcagga cgctgaattc cgtcttgatg gcggggccat
      421 gtttggcgtg gtccctcgca aactgtggtc ccaggtctgt ccgcccgacg acgagaaccg
      481 catccggatg aacatgaact gcgtgtttgt cgataccgga tcggagaagg tgctgatcga
      541 aaccgggatc ggcgataagt ggccggcaaa acagtcagcg atgtacggca tccatcgcgc
      601 gcgtgctttg actgacacgt tgtttgaaca gaccggtgtt tccgctgacg acgtgacgat
      661 tgtggtcaac acgcacctgc atttcgatca tgcaggcggc aacactcgct tggacagcga
      721 cggcaacgcg gtggcgttgt ttccaaacgc tcgttatttc gtttcgcgcg ctgagttggt
      781 acatgcggag gagccgacgg agcgcgatcg tgcgagttat ctgccggaga actggcgacc
      841 gctcgttgct tcaggacaac tggaaccaaa ggacgcgaaa tacgaagttg tgcctgggct
      901 aacgatggaa acaatcgccg gacacaaccg ctcgatgcag tgctggcgtc ttacgcgtga
      961 cgggaagacg atgtttggct ttgccgatct cgtaccaacg cgagcgcatc tacggctgcc
     1021 gtggatcatg ggttacgacc tttatccggt ggaaacgctc gcggccaaga aacgactgtt
     1081 gccggaagcg gcgcggaaca actggctttg cttgttctat cacgatccgg acgagccggt
     1141 ttgtcgtttg cgggaaggcg aaaaggaatt tgtgcccgtt ccttatcgcg gagaggaaaa
     1201 gaacggttca tgagtaatcg tccacgagtg cgctttggca tcatcggcgg cagtggtctc
     1261 tatcagatgc cggagttgac ggccgttgag gaagtcaagg tgcctacgcc tttcggagag
     1321 ccttctgatt cgtttgtgat cggaactctc gaaggtgaac gcgtcgcgtt tctgccgcgt
     1381 cacggcgtcg gtcatcgtta cacgccgact gaagttccgt ttcgcgcgaa tatctatgcg
     1441 atgaagttgt tgggtgtgga acgaattctc tcggcttcag cggtcggatc gttgcaggaa
     1501 aagtatgcgc cgctcgacat ggtgattccg gatcagtttt tcgatcgcac gcgtgggcgc
     1561 gttcccgagt cgacgttctt cggtgacggg attgtggcgc atgtttcctt cgcacatccg
     1621 gtttgttccg cgttgggtga tgtgttggag gaatcgtgtc gcgttgctga cgtgaagacg
     1681 catcgcggtg gcacgtactt gtgcatggaa gggccggcgt tttcgaccgt ggccgaatcg
     1741 aatgtctatc gctcgtgggg catggacatc atcggcatga cgaatttgca ggaggctaaa
     1801 ctggcgcgcg aagcagagat ctgttacgcg actctcgcgc tggtgacgga ttacgattgc
     1861 tggcacccgg gccacgatgc cgtgagcgtc gagaccgtgg ttgagtactt gaataagaac
     1921 gtgcgcaacg cgcagattat tctgcgcgaa gcagtaaagc gtctgaagaa cagtgagcgc
     1981 acttgcaagt gcggttctgc gttgcagcac gcgatcttca ctcaaccaag tctctggccg
     2041 gcagagacgg cgaagaagct ggaagcaatc gttagaaagt accagggatg aagggcattt
     2101 tcgattgccg attgccgatt gtcgattgcc gatttgaaac gccgggaggt gtagccaacc
     2161 gagggagcaa atcggcaatc gacaatcggc aattggcaat cggcaattgg caatacgtaa
     2221 gaggtgagct atgaaaggca gttttgtatt gatgttgatc tttgcagcag tgctgcttgt
     2281 gccggcgccg gcggcgcagg cccagaccgt gcgcaccgga cccgatccga acacgctgcg
     2341 cgatcccgat ctcgagaaag attcacagca taatctggaa gtggcgaaaa actacttcaa
     2401 actgaagaag gcttatgtcg cggcgctgaa acgttgtgaa gagatcatcg ccggcaatcc
     2461 caacttcgcg aagatcgaag aagtgctgct gatggcgggc caaagcagtt tgtggctgtc
     2521 tgagaaaaaa ggtaaacaga gtcccgatct gtatgtcact ttcgacggtg gcgagagacg
     2581 cacgctaaca tccgaacagt tccgcgaaat gggccgcgag tccctgagca agttgataaa
     2641 ggactatccc gacggctcct acactaaaca agcgcgcgaa gagttacgtg cgcgctcgct
     2701 cgaggccaaa ccctaaacca tgtcccttct agttgttggt tccgttgcct acgacgcagt
     2761 tgaaactcct ttcggtaagt gcgagaagat gctcggaggt tccgcctcgc acttctccat
     2821 ttccgcgagc ttttttaccg acgtcagtgt cgttgccgtg gttggcggcg actttactga
     2881 ctttgacatt tttgaaaaac atcgcatagg aacgcgcgac ctcgaatgta tcgacgacgg
     2941 caaaaccttc cgttggggag gacgctacga ttacgacctt aacgtcgcgc acacgttgtt
     3001 taccgacctg aacgtgttcg cagattttca gccaaagctg tctgaggagt cgaaacgctc
     3061 gcggctggcg tttctcggca acattcaacc ggacttgcag cgcggtgttc gcgaacagct
     3121 gcctgatgcg gaactcgttg cgctcgacac gatgaatctc tggatcgagc acacgcgcga
     3181 gtcgctgttg cgcacgatcc gtatcgtgga cgttgtgatc atcaacgacg cagaagcccg
     3241 acagctcacc ggcataccaa acctcatcaa ggctgcgcgc gagattctga gttggggacc
     3301 aaaggcgttg attatcaagc ggggtgaata tggcgcggcg ctgttcacgg ctgacagtta
     3361 ctttgcgatt ccggcgtatc cgctcgaggc tgttttcgat ccgaccggcg cgggcgacac
     3421 gttcgcgggt ggtttcatgg gctatttggc gagccaggag cgcctggacg aggacgcaat
     3481 gcgccgtgca atgatcttcg ggtcggtgat ggcgtcattc aatgttgagg agtttggcac
     3541 tgagcgcgtg cgccggctca gccatgcaga gattaacgat cgttttcgcg ccttcaaacg
     3601 aatgacacat tttgatgaga tacctttcgt tgcgagggtt tgagctttgt tcttggtact
     3661 ttgtattttg tattgttttt gttcttggag tacaaagaac aaagtacagc ccccgtcagg
     3721 agacacgtct aaaaatgttc tgcccaacat gtgaatcaga atacgaaccc ggtattaccc
     3781 gctgtcctga cgatgagacg ctgctggtcg agcggctcac gcctgagaac accctgcacg
     3841 acaacagcga ggcgcggttt gtgtcgttct acaagctcgg ttcgccggcc gaagccgaga
     3901 tggtcaacga cattttccaa aagaacgaca tccgctccgt gataaaatca ggcggcgctg
     3961 acgcgttttc accattgttg tcggcaacct ccgagggtgc ggagatcttc gtagacgagc
     4021 gagacgttga tcgcgcggcg gaaatctaca acgcattctt tggcgccgac acgacaccac
     4081 tcactggtgg accactcgaa gaagatgaag agcagtagca atgccggaca aagacgcaat
     4141 tgtagttttc atgacggccg cgaacggtga ggaagccaca cgcctcgcag agctcctcat
     4201 cggcgcgcac ctcgccgcct gcgtgcaaat cctgccggaa atggaatcgg tctatcgctg
     4261 gcggggaaag atcgagcgcc agtcagagat tctgctaatc gtgaagacga cgaccgataa
     4321 gtttgcggat ttggaaagag aagtgcgcgc cttgcacagt tacgacacgc ccgagatcat
     4381 cgcagtcccg atcgttgccg gttctacgtc ctacctcgaa tggctcgatg acagtttgaa
     4441 gccttagctg ggaatgcgga tgtcgtcaga aacgagcttg tccagttcgg cgagtgagct
     4501 ctgacttcga ttgaggaact ccaaaacatc cttcggcgaa tcggtcacat gaaacatgcg
     4561 caggtcgtga ctcgagatct taccgtctgt cagcaccacc tgccgtaacc aatccagcaa
     4621 cccgctccag tagctactgc cgaaaagcac caccgggaag tttcggatct tctttgtctg
     4681 gatcagcgtg agcgcctcga agagttcgtc aaacgtaccg aagccgcctg gaaagatgac
     4741 gaagccaagg ctgtacttca caaacatcat cttgcgcacg aagaagtatt tgaacttcag
     4801 actgcgcgtt tgatacgcgt tcggtcgttg ttcgaagggc aattcaatgt tgcagccgat
     4861 ggacagaccg ccggcttcga aggcgccgcg attggccgcc tccatgatgc ctggcccacc
     4921 accggtgatg actgcataac cttccgccgc gagcaaggcg gcggtttcga gcgccgcctg
     4981 gtaatcggga tcacccggct tggaacgcgc cgagccgaag atcgagatcc cgcgcgtgag
     5041 tgctgcgagt tcgtcaaagc cttctacaaa ctcacccatg atgcgaaaca cgcgccacgg
     5101 atccgtgtgt gtaaactcgt ctggtcgcgg actctcgagc agttgctcgt cctgggtgga
     5161 gccggtaggc gcgcgatcga tctcgtttga ctcgtcttcc atcgtttccg tcagatgccc
     5221 atgatgttgt aaccacaatc gacgtagatc acttcgccgg taattcccga agccatagag
     5281 ctacagagaa agacggcggt gtttcccacc tcgcgcggct ctacatttcg gccgagcggc
     5341 gcgcgttcgg cgtggtgctt caacatcgtg ctgaagtcgg caacgccgcg cgcggagaga
     5401 gtttgaattg gaccagcgct gatggcgttg atacggatct tgcgcggtcc aagatccttg
     5461 gcgaggtagc gcgtgcttgc ttcgagtgcg gctttggcta cacccatcac gttgtaaccg
     5521 gcgacgacct tttcggcgcc gtagtaagtc atggtgacga tgctgccgcc ttcggtcatt
     5581 aaaggcgcag ccgcgcgcgc gagttgcgcg agggaatagg cactgatctc gagtgcggta
     5641 agaaacgcct cgcggctggt gt
//
LOCUS       DASTEY010000668         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_592248, whole genome shotgun sequence.
ACCESSION   DASTEY010000668 DASTEY010000000
VERSION     DASTEY010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592248"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1006
                     /locus_tag="VFO72_11625"
     CDS             293..1006
                     /locus_tag="VFO72_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9479990.1"
                     /translation="MPISDSQTEHVSDIEAALLDAELFMKYQAPDRAIKRLRTALEVN
                     SRSIKLRERLREICAAHKQVEEAARQCLALASLYIEREDFETAHDRLIEAKQFDERIN
                     IAGGLEAIRRARRPDLKPTPAPEAQFERKHFTLAGDLSAISVFDAMQVIENARLNGVL
                     TLNGPKYEGTVKFNEGRIVDAVCDGQVGEMGFQRVVEITSGTFEFQKSSEQYPVRIQA
                     LSNTNLILDTLRLLDEKKQ"
     gene            complement(1010..1771)
                     /locus_tag="VFO72_11630"
     CDS             complement(1010..1771)
                     /locus_tag="VFO72_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479991.1"
                     /translation="MSLEFALQYACPLRANYSERHLREWGRLSSLHAKLTTGDASAPS
                     IDKLLWVENASAEIERMQEATTVCAQCPACLPLDTAGDGESVGCLGRINYPVEAQFEK
                     FLADRVQLALDTLDKEDQPRLLRILIDPESPFDGEATKELRRVVTEDGLRFFELRVPI
                     VLTREAARLTTDNIFDLLAGFRSEDSEMTTYTRELPYEAAADYYDFLDLILRNELSQS
                     EVARLTSRSRNYNQFLRLLTAIEKAEALNTRVLID"
     gene            1877..2047
                     /locus_tag="VFO72_11635"
     CDS             1877..2047
                     /locus_tag="VFO72_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET9479992.1"
                     /translation="MAVSQELLEILACPACKAKVELKPDGNALKCVECKRVYPIRDDI
                     PVMLIDEATIEE"
BASE COUNT          503 a          604 c          589 g          465 t
ORIGIN      
        1 tactactgaa tttcggcgag aagcctgagt tcaagagagt gattttcgac aacctcagaa
       61 agatccgcaa ataatccgtg tttatctgtg gcgaaatttc tcgcagcggg cccgagaacg
      121 ctcaattcgt cttttcatcc accggttcat tgtcttgcac cttccaattt ctctgatgtt
      181 cttcctacga ttgcgttgcc ccgcaggttg catgccaatc gtatcttccc acctgtaacg
      241 cgaattttcc ggaaaaaaga gaccattttg gtgtgataaa ctcccaattg gtatgccgat
      301 ctcggactca caaactgagc acgtcagcga tattgaagcc gctttgctcg acgccgagct
      361 cttcatgaag taccaggcgc cggaccgtgc cataaaaaga ttgcgaacgg cgcttgaagt
      421 caacagccgc tcgatcaaat tgcgcgagcg tttgcgcgag atttgcgccg cccacaagca
      481 ggtcgaagag gctgcccgcc agtgccttgc cctggccagt ctttatatcg aacgcgaaga
      541 ttttgagacg gctcacgacc gcctgatcga agcgaagcaa tttgacgagc gaataaatat
      601 cgccggtggt ctcgaggcga ttcgtcgcgc ccggcggcct gacctgaaac ctacacccgc
      661 tcccgaagcc cagtttgaac gcaagcactt cactttggcc ggggatctca gcgcaatcag
      721 cgtcttcgac gcaatgcaag tgatcgaaaa cgcgcgtttg aacggcgtac tgactctcaa
      781 cggtccaaaa tacgaaggca cggtgaaatt caacgagggt cgtatcgtcg acgctgtttg
      841 cgacggtcaa gtcggagaaa tggggtttca gcgagtagtg gagattacga gcggcacgtt
      901 cgaatttcag aaaagctcgg aacagtatcc agtgcgtatt caggcgctca gcaacaccaa
      961 cctcatcctc gacaccttgc gtctgttaga cgagaagaag cagtaaacgc tagtcgatca
     1021 acacacgagt gttcaacgct tccgcttttt cgatcgcggt gaggaggcgt agaaactggt
     1081 tgtagtttcg actacgcgaa gtcaggcgcg caacttccga ctggctcagc tcgttgcgca
     1141 ggatgagatc gagaaagtcg taatagtcgg cggcagcctc gtaaggaagc tcgcgtgtgt
     1201 acgtcgtcat ctcactgtcc tcagaacgaa agcccgcgag cagatcgaag atgttgtccg
     1261 tcgtcaggcg cgcggcttcg cgcgtgagaa cgatcggcac gcgcaactca aaaaaccgca
     1321 acccatcttc ggtaaccacg cgccgcaact ccttcgtcgc ttcgccgtcg aaaggcgact
     1381 ccgggtcgat taggatgcgc agcaatcgcg gctggtcttc cttgtcaagc gtgtcgagcg
     1441 cgagctgcac gcggtcggcg agaaactttt caaactgcgc ctcaaccgga taattgatcc
     1501 gacctaaaca gcctaccgac tccccgtcgc cggccgtatc gagcggcaga cacgcgggac
     1561 actgggcaca gacagtcgtc gcctcctgca tgcgctcgat ttcggcgctg gcgttttcaa
     1621 cccacagaag tttgtcgatt gagggcgcgc tggcgtcgcc agtggtgagc tttgcgtgca
     1681 ggctgctcag gcgaccccac tcgcgcaagt ggcgctcaga gtagttggcg cggagggggc
     1741 aagcgtattg cagtgcgaat tccagcgaca tcgttgaaag tataggtcat ataacaccta
     1801 taggtcatat acgacctatc ttgctctttg tgataagctt tccccatccc gcagcatgcc
     1861 taggaaaggt cagcccatgg cagttagtca ggaactctta gagatactcg cgtgtccggc
     1921 ttgcaaggcg aaggtcgagc tcaagcccga tggcaatgcg ctgaagtgcg tcgaatgcaa
     1981 gcgtgtttat cccatccgcg acgatatccc ggtaatgctc atcgacgagg ccacgatcga
     2041 agagtgattc gatgaacagc ttgcgcgaca tcgtcgagct ttctcctcag ttacgcgaca
     2101 tcgaaacaca agcaatgtat ggattgcagg gtcaagacgc cgtgcagccg ctggcgccgc
     2161 c
//
LOCUS       DASTEY010000669         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_724268, whole genome shotgun sequence.
ACCESSION   DASTEY010000669 DASTEY010000000
VERSION     DASTEY010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724268"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2081)
                     /locus_tag="VFO72_11640"
     CDS             complement(<1..>2081)
                     /locus_tag="VFO72_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9479993.1"
                     /translation="AVPIGRPIANTQAYVLDQLQRPLVAGARGELYLGGANISRGYLG
                     DPRLTAEKFIPDPFSFVPGARLYRTGDLARWRHDGQIEFLGRVDQQVKIRGHRIEPAE
                     VETALLQHEAVRDAVVVAREEANGERRLLAYVVADRDPLTRRVSRAKAPSRKETLRKG
                     GSALRLGAFARETSPSSEDRRRDPEYFLCKAELRSFLTDLLPAHMIPASFTLLEALPR
                     LPGSKIDRRALPQPDLSCADGSFVAPRTSVERVLCELWAEVLKLERIGIHDNFFELGG
                     DSILSIQVAARARLAGLQLSPTQIFQHPTPAQLAAVTQISRASHAVPESTTGNVPLTP
                     IQHWFFEQHFPFPDYWNMALMLKARERLDPGLLEQAFAHLVHHHDALRLRFNASGAPR
                     QFIADGDPGANGNILHVVNLSDVVENEQQSFLETIADETQAQLDVAKGVVLRAVLFEL
                     GEGQPPRLLIVIHHLVVDGVSWRILLEDLGRTYRQLQRSEAVSFPPKTTPFTRWASLL
                     SENARSAQVRNELDYWTRLSAKHVKPLPIDFRRGSNTEAEARALTIALDASETRALLR
                     DVGHAYGTQINDVLLTALLDALSHWTSEQNILVELEGHGREDLFDDVDLSRTVGWFTN
                     AFPVLLEATAGLSSVAALQSVKRQLRSIPAHGIGYGLLRYLNDDAEVSGQMRDLPEPE
                     VSFNYLGQLDQ"
BASE COUNT          461 a          606 c          598 g          416 t
ORIGIN      
        1 tctggtctaa ctggcccaga tagttgaaac taacttcagg ttcaggcagg tcgcgcattt
       61 gacccgagac ttcggcatcg tcgttgagat aacgcaacaa tccgtaacca attccgtgtg
      121 ctggaatact gcgcaactgt cgtttgaccg actgcaacgc cgccacggag gagagaccgg
      181 ctgttgcttc cagtaagacg ggaaacgcgt tggtaaacca gcctaccgtt cgcgagagat
      241 ccacgtcgtc gaaaagatcc tcgcgtccgt gtccttcaag ctcgaccagg atgttctgtt
      301 cgctggtcca atgcgagagg gcgtcgagca aagctgtcaa caacacgtcg ttaatctgtg
      361 tgccgtaagc gtggccgacg tctcgcagta gcgcccgtgt ctcggaggcg tcgagcgcaa
      421 tcgtaagagc tcgagcttca gcctccgtgt tgcttccgcg acggaaatcg atcggcagtg
      481 gcttgacgtg tttcgcggac aacctggtcc agtaatccag ttcgttgcga acttgtgcgg
      541 aacgcgcgtt ttcggaaagc aaactcgccc agcgtgtaaa aggggttgtc tttggcggaa
      601 agctcacggc ctcgctgcgc tggagctgcc ggtaagtgcg gcccagatct tccaacagga
      661 ttcgccacga gacgccgtca acgacaagat gatgaatgac aatcaacagc cgtggcggct
      721 gaccttcgcc aagctcgaat aacacagccc gcagcaccac gccctttgcg acatcgagct
      781 gagcttgtgt ctcgtctgca atcgtctcca aaaaagactg ttgctcgttc tcgacaacgt
      841 cagacaaatt gacaacgtgg agtatgtttc cgtttgctcc cggatcgcca tccgcgatga
      901 attgacgtgg cgcgccagac gcgttgaacc gcagccgcaa cgcatcgtga tggtgcacga
      961 ggtgtgcgaa cgcctgctcc aacaggccgg ggtcgagacg ctcgcgcgcc ttcagcatca
     1021 gagccatgtt ccagtaatcc ggaaagggaa agtgctgctc gaagaaccaa tgctgaatcg
     1081 gtgtcaacgg tacatttccg gtagtacttt ccggcacggc gtgactcgca cgggagatct
     1141 gagtaacagc cgccaactgg gccggtgtgg gatgctggaa gatctgagtt ggtgatagtt
     1201 gcaggccggc gaggcgagcg cgggccgcca cttgaatgct gagaatcgaa tcgccgccca
     1261 gttcaaaaaa gttatcgtgg atgccaatgc gctccagctt gagaacttca gcccagagct
     1321 cgcacagcac gcgttcgacc gaagttcgcg gcgcaacaaa agatccatct gcacaagaga
     1381 gatccggctg tggcaacgcc cggcgatcga tcttgctacc cggcaaacgt ggcaacgctt
     1441 ccaacagcgt gaagctcgct gggatcatgt gcgccggcaa aagatccgtt aagaagctgc
     1501 gcaactccgc tttgcacaaa aagtactcag gatcgcgccg gcggtcctct gaggaaggag
     1561 atgtttctcg cgcaaaggcg ccaaggcgca aagcgctacc gccttttcta agggtttctt
     1621 tgcggcttgg cgcctttgcg cgagaaactc ttctggtcag agggtcccta tcggcgacga
     1681 cataagcgag caatcttcgc tcaccattcg cttcttcacg agcgacgaca actgcgtcac
     1741 gcaccgcttc gtgctgcagc agcgcggtct caacctcggc cggctcgatg cgatggccgc
     1801 gaatttttac ctgctggtca acgcgaccaa gaaactcgat ctgtccatcg tgcctccacc
     1861 gtgctagatc acccgtgcga tagagtcgcg cgccggggac aaaactgaag ggatcgggaa
     1921 tgaatttctc cgccgtcagt cgcggatcgc caagatagcc acgactgatg ttcgcaccgc
     1981 cgaggtacaa ctcaccgcgc gcacccgcaa ccagcggccg ttgaagctgg tccaacacat
     2041 acgcctgggt gttggcgatc ggtcggccga ttggcacggc t
//
LOCUS       DASTEY010000670         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_114164, whole genome shotgun sequence.
ACCESSION   DASTEY010000670 DASTEY010000000
VERSION     DASTEY010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114164"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..746)
                     /gene="cobO"
                     /locus_tag="VFO72_11645"
     CDS             complement(174..746)
                     /gene="cobO"
                     /locus_tag="VFO72_11645"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9479994.1"
                     /translation="MKEKKYKWRREEYQENTRGLLMVHTGNGKGKTTCALGLMMRAAG
                     RGLRCCMIQFMKSRNDRYGEHVSAEKLGIEVHTMGDGFTWDTKNPEQDIKTARETWAL
                     CVKKLRSGEYDLLVFDELVYVLSYKMLPAEEVLEEIRSIRASQPALHIVVTGRDAPAE
                     LIEAADLVTEMREIKHPFTAGIRGQQGIEF"
     gene            complement(752..1753)
                     /locus_tag="VFO72_11650"
     CDS             complement(752..1753)
                     /locus_tag="VFO72_11650"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET9479995.1"
                     /translation="MLSQVVELIESKRRFAITSHIRPDGDSLGSSLGLYWLLRALDKD
                     VEVIMRDPVPHAYQQLPGAGDVRVTPVVEPSYHAVFVIECSDITRPGLIDLEKQFVVN
                     IDHHSTTALFGSINWIDSTASAVGEMIYNLCKATGVRVTKEIAECVYTALITDTGSFH
                     YSNTTERTFKVASELVRTGVKPAKTAEAVFASYPWSRIELMGAVLSTARRDETGRVAY
                     MRQSLDMQRQANASDEDADGFVNYPLTVGEVEAVALLKECEPGVYRTSLRSKGDVNVA
                     KVAEKFGGGGHRNAAGCTLRGSWEEAETVIVSLLQEAVKRADGLNDITEDALKESVV"
     gene            complement(1958..2320)
                     /gene="rbfA"
                     /locus_tag="VFO72_11655"
     CDS             complement(1958..2320)
                     /gene="rbfA"
                     /locus_tag="VFO72_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9479996.1"
                     /translation="MAIGNFMHRTERFTDALREEIAQIVGYELEDPRVSTVTVTDVRL
                     SSDKRAARVYVTVAGDEAEHKQALNALKHAAPYVRKQLGLSLNLPRTPELHFVRDRVE
                     EQGERVDQLLREIELEKE"
BASE COUNT          531 a          766 c          665 g          605 t
ORIGIN      
        1 aggttcattg cgcgattgcc acttgggtca ttggcgattt tcgattttcg attgccgatt
       61 gccgattttt tccgaaccac cggttcattg cccggctgcc ttcacgattg ccgagtcaag
      121 gcggcgtcag tcggtgcaaa aatcgtcaat cggcaatcga aaatcggcaa tgactaaaac
      181 tcgattcctt gctggccccg gattccggcg gtgaagggat gcttaatttc gcgcatttcc
      241 gtcactaaat cggcggcttc gatcaattcc gcgggtgcgt ctctgcccgt cacgacgata
      301 tgcaaagccg gttgtgaggc tcggatagaa cggatctcct ctaaaacctc ctcggccggc
      361 aacattttgt aactcagcac gtagacgagc tcgtcaaaca ccagcagatc atattcgccg
      421 gagcggagct tcttgacgca caaagcccac gtctcgcgcg cggtcttgat atcctgttcg
      481 gggttttttg tgtcccaggt gaagccgtcg cccatcgtgt ggacttcgat accgagcttc
      541 tcagcggaga cgtgttcgcc gtagcgatcg ttgcgcgact tcatgaactg gatcatgcaa
      601 caccgcaaac cgcgtccagc ggcgcgcatc atcaaaccca gcgcacaggt cgtcttgccc
      661 ttgccgtttc cggtgtggac catcaagagg ccacgggtgt tctcctgata ctcctcccga
      721 cgccacttgt actttttctc tttcaacgcg cttacaccac ggattccttc agcgcgtctt
      781 cggtgatgtc gttgagtcca tccgcccgct taaccgcttc ctgcaacaga ctcacgatca
      841 ctgtctcggc ttcttcccag cttccgcgca acgtgcagcc agcggcgttg cgatggcccc
      901 cgccaccgaa tttttcggcg accttcgcca cgttcacgtc gcctttagaa cgcaaactgg
      961 tacggtaaac tcccggttca cactccttca gcaacgccac cgcttcgacc tcgccgaccg
     1021 tcaacggata gttaacaaat ccatccgcgt cttcatcgga agcgtttgcc tggcgttgca
     1081 tgtcgagcga ctgccgcatg taagcgacgc gtcccgtctc gtcacgtctg gcagtagaca
     1141 acactgcgcc catcaattcg atcctgctcc acggataact ggcaaagacg gcttcggcgg
     1201 ttttcgcggg tttgacgccg gtgcgaacca gctcggaagc aactttgaac gtgcgctcgg
     1261 tggtgttgga gtagtgaaac gatccggtat cggtgatcag cgcggtgtag acacactcgg
     1321 cgatctcttt agtcacgcgc acgccggttg ccttgcagag gttgtagatc atctcgccga
     1381 ctgcggacgc cgtcgaatca atccagttga tgctgccgaa aagcgcggtg gtcgaatggt
     1441 gatcgatatt gacaacaaac tgtttctcga gatcgatcaa tccggggcga gtgatatcgg
     1501 aacactcgat gacaaacacc gcgtggtaag aaggttcgac tacgggcgtg acccgcacgt
     1561 cgccggcgcc gggcagttgc tggtaagcgt gcggcactgg atcacgcatg atgacttcaa
     1621 cgtctttgtc caatgcgcgc agcaaccaat agaggccgag cgatgaaccg aggctgtcgc
     1681 cgtccgggcg aatatgtgag gtgatcgcga aacgacgttt tgactcgatc agctcgacta
     1741 cttgactaag catgattcgt tctttgaacc ttgtactttg ttctttgttt gctccgaagg
     1801 agcaaaatgt ttatagacgt gcgccacgac catctattgc gctccttcgg agcgcgatgt
     1861 acggggccgg atgagcccgc tcctccggag cacggggtaa gcccgaccat gagcacaccg
     1921 tcagcctaac aaagaactaa gtaccaagtt cgaaacacta ctccttttcc agttcaatct
     1981 ctctgagcag ttgatcgact cgctcgccct gctcttcgac gcgatcgcgt acgaagtgaa
     2041 gctcaggcgt gcgcggcaga ttcaacgaaa ggccaagttg cttgcgaacg tagggcgcag
     2101 catgtttcaa agcattcagc gcctgcttgt gttctgcttc gtcgccggcc acggtgacgt
     2161 aaacccgcgc agcgcgcttg tcggacgaga gacggacgtc agttacggtt acggtgctca
     2221 cacgcgggtc ttcgagttcg tagcctacga tttgcgcaat ctcctcccgc aacgcatccg
     2281 taaatctctc tgtccgatgc atgaaattgc cgattgccaa ttgccaattg ccaattgctg
     2341 atttctgaaa ccttttgccg ctcttcaatc aaaatcggca atcggcaatc ggcaatcggc
     2401 aatgcctaca gttccgtcgc cgcgactcgc tcgacgacgt atgcctcaat ctggtcgccg
     2461 actttcacgt cgttgaagtt ttccagactg atgccacatt caaacccttc gcgaacttcc
     2521 gatacgtcat ccttgaaccg ccgcaacgac gcgatgttgc cttccca
//
LOCUS       DASTEY010000671         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1202015, whole genome shotgun sequence.
ACCESSION   DASTEY010000671 DASTEY010000000
VERSION     DASTEY010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202015"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..883
                     /locus_tag="VFO72_11660"
     CDS             5..883
                     /locus_tag="VFO72_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9479997.1"
                     /translation="MSEDTKQPGSEEPTKPGVSDSEASGTPPPSAIPPAGALAGADAE
                     KASMVEQAKSRVAAVKESLTEQAAAPGAPKPPVKKKEEGPKPTDASEHALVKKLKAQF
                     GEAIGEASEFIGQLSVYVDGQRIVEVCDFLKSDADASFNYLSDLTCVHYPQRRDAPFE
                     MVYNLFAIEANERVRLKVRTKERVDSVTGVWPSANWLEREVFDLFGVRFNNHPDLRRL
                     LLPPDWEGHPLRKDFPLEFVENAWTAKHLPVMTQVEMEQLQQRRAYGLEILSVPEERM
                     MRQLFIGGKEVMPKDK"
     gene            880..1140
                     /locus_tag="VFO72_11665"
     CDS             880..1140
                     /locus_tag="VFO72_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9479998.1"
                     /translation="MTPKEIATHYDAKVFDTPEAAKVAGFVLTETAAPRNVWNKASAA
                     SAIVLKLLEKRESGEVDDIGLVIEPFRVTGCYKPHPAETPAA"
     gene            1146..>2149
                     /locus_tag="VFO72_11670"
     CDS             1146..>2149
                     /locus_tag="VFO72_11670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9479999.1"
                     /translation="MHQVEIATTQETLLDSPEMVLNMGPQHPSTHGVLRVVLRLDGET
                     VIDLDCDIGYLHRGMEKIAENRMYTMIAPYWDRLDYIAAVSNGLVWVETVEQMMGLPV
                     PPRAHYLRTILTEFNRIASHLLWLATHALDIGAVTVLLWALREREEILKIFERQFGAR
                     LTCHAWRVGGMPNDAYDDFNKDVRQFVNNFPKRLDEYETVLSENRIWLSRTVGIGVIS
                     AEDAIAIGLSGPALRGSGVAWDVRKSRPYAAYADMDFDIPLGENGDTYDRYLVRLEEM
                     RQSRRIIDQALENLPDGPVNAKLPKVVKPPAGDYYHSIEAPKGEIGCYIVSDGTAQPW
                     R"
BASE COUNT          498 a          565 c          667 g          419 t
ORIGIN      
        1 atagatgtca gaagacacga agcaacctgg ttctgaagaa cccacaaagc cgggcgtctc
       61 agattctgag gcgtccggca cgccaccccc atcggcaatt ccgcccgcag gagcgctcgc
      121 cggagccgac gctgaaaagg cgtcgatggt cgagcaggcg aagtctcgtg tggctgcggt
      181 aaaggaatcg ctaaccgaac aggctgccgc acctggagcg ccgaaacctc ccgtcaagaa
      241 aaaagaagaa ggtccaaaac ccaccgacgc cagcgaacac gcgctggtca agaaactgaa
      301 agcacaattc ggtgaggcga ttggcgaagc gtctgagttt atcggacagc tttcggttta
      361 cgtcgatggg cagcgcatcg tcgaggtctg cgattttctc aaaagcgatg cggatgcgag
      421 tttcaactac ctttccgatc tgacgtgcgt gcactatccc cagcgcagag acgcgccgtt
      481 tgaaatggtc tacaacctgt ttgcgatcga agcgaatgag cgagtccgat tgaaggtcag
      541 gacgaaagaa cgtgtagaca gcgtgaccgg cgtttggcct tctgcgaact ggctcgagcg
      601 cgaagttttc gatctgtttg gtgttcgctt caacaatcat ccggatctgc gccgcctgct
      661 cttgccaccg gattgggaag ggcatccgtt gcggaaagat tttccgctgg agtttgtgga
      721 gaacgcctgg acggcgaaac acttgccggt gatgacgcaa gttgagatgg agcagttgca
      781 acagcgacgt gcttacgggc tcgagattct ttcagtgcct gaagaacgaa tgatgcggca
      841 gttattcatt ggtggcaagg aagtaatgcc gaaggacaaa tgacgccaaa agagatcgcc
      901 actcattacg atgcgaaggt gttcgacacg cccgaggcgg caaaagtggc ggggtttgtt
      961 ttgaccgaaa cggcagcgcc gagaaacgtc tggaacaaag cgagcgcggc gagtgcgatc
     1021 gtgttgaagt tgttagagaa gagagaaagc ggcgaagtgg atgacatcgg attggtgatc
     1081 gagccgtttc gcgtgactgg ttgttacaaa ccacacccgg ccgagacgcc cgccgcgtaa
     1141 taaagatgca ccaggtagaa atagcgacaa ctcaagagac actgctcgac agcccagaga
     1201 tggtgctgaa catgggcccg cagcacccgt caacgcacgg agtgttgcgc gtggtgctca
     1261 ggctcgacgg cgagaccgtt atcgatctcg attgtgacat cggttatctg catcgcggca
     1321 tggagaagat cgccgagaac cgcatgtaca cgatgatcgc gccctactgg gatcgactcg
     1381 actacatcgc ggcagtctcg aacggcctcg tgtgggtgga aacggtcgag caaatgatgg
     1441 gacttccggt cccgccgcgc gcgcactacc tgcgcacgat tctcacggag ttcaatcgca
     1501 tcgcttcgca cctgctctgg ctggcgacgc acgcactcga catcggcgcc gtgaccgttt
     1561 tgctgtgggc gctgcgcgag cgcgaagaga ttctcaagat ctttgaacgg cagtttggcg
     1621 cgcggcttac gtgtcacgcg tggcgcgtcg gcggcatgcc aaacgatgcc tacgacgatt
     1681 tcaacaagga cgtccggcag ttcgtcaaca actttccgaa gcgactcgat gaatacgaaa
     1741 cggtgctttc tgagaatcgc atctggttga gtcgaacggt cggtatcggc gtgatctccg
     1801 ctgaagatgc gatcgcgatc ggcttgtcag gaccggcgtt gcgtggttcg ggagtcgcgt
     1861 gggacgtgcg gaagtcgcga ccgtacgccg cttatgccga catggatttc gacatcccgc
     1921 tcggtgagaa tggcgacaca tatgatcgtt acctggtgcg gctggaagag atgcgacagt
     1981 cgcgccgaat catcgatcag gcgttggaaa atcttcccga tggaccagta aacgcgaagt
     2041 tgccgaaggt cgtgaagcca ccggcgggtg actactacca ctcgatcgaa gcaccgaaag
     2101 gcgagatcgg gtgttacatc gtttcggatg gcactgcgca gccgtggcg
//
LOCUS       DASTEY010000672         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_262751, whole genome shotgun sequence.
ACCESSION   DASTEY010000672 DASTEY010000000
VERSION     DASTEY010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262751"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1221
                     /locus_tag="VFO72_11675"
     CDS             43..1221
                     /locus_tag="VFO72_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9480000.1"
                     /translation="MSQSQTALAQSPTPLTTLSEDETMFRASVREFAEGELRPRVEHM
                     DETAKLDPAIINQCFQLGLMGIETPEEYGGAGANFFSAILAVEELSRVDASVGVFVDV
                     QNTLVNNALIRWGTNEQKQKYLTRLASEHVGAYALSEPSSGSDAFAMQTRAVDKGDHY
                     VLNGRKLWITNGIEAEIFVLFANANPEAGYKGITAFIVEKSSPGFSIGKKENKLGIRA
                     SSTVELILEDCEVPKENVLGEAGKGYKISIETLNEGRLGIGAQMIGIAAGALESALAY
                     TAERQQFGKSINQFQGVQFQLAEMATELEAARLMVYNAARMKDAGMNFVKEAAMAKLY
                     SSRVAERVSSKAIELYGGYGYVKDYPVEKYWRDSKIGAIYEGTSNMQLQTIAKLIIGK
                     "
     gene            1218..1535
                     /locus_tag="VFO72_11680"
     CDS             1218..1535
                     /locus_tag="VFO72_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480001.1"
                     /translation="MNNRIELFQQMLTNDPGNTTVLFGLAKEYEKAGRDQELIETLNR
                     YLEMVDDEGNAFGMLAGAYERLGKRDEARKAYERGIEAAQRHGHPGMAEEYRMTLAGN
                     YEH"
     gene            1525..>3098
                     /locus_tag="VFO72_11685"
     CDS             1525..>3098
                     /locus_tag="VFO72_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9480002.1"
                     /translation="MNTDTKVLKLVRKADSTAEAVDNSAVQPAPRAVPASVSKEEQLP
                     DHKLLLEMYRTMYLSRRIDDKEIQLKGQNRIFFQISGAGHEAVLAAAGVVLKPSYDWF
                     YPYYRDRALCLQLGMTPAEQLLSSVGAEADPNSHGRQMPSHWGHKKLNIVSQSSPTGT
                     QLLQAVGCAEASYRFHLIPELKKRIEDFHDDEVVYVSLGEGTTSEGEFWEALNTACNL
                     SLPVVFVVEDNGYAISVPVEVQTAGGDVSRLVEGFPSLYIQRCDGTDAIESLEVMRRA
                     VDYCRQRKGPSLVHAKVIRPYSHSLSDDEKLYRPDEERASDAERDPVKRFGALLIDSG
                     VIDQQGLQRIKDEADREVNEAADIALASVQPAPDTAMKYVFSPDVDPTSKEFDTEDQV
                     QLSGNPGTMVDLINRCLHTEMARDPRIVVFGEDVADCSREKYLGAVKGKGGVFKVTAN
                     LQRNFGGARVFNSPLAEANIVGRAIGMATRGLKPVVEIQFFDYIWPAMHQIRNELALM
                     RWRSANDWKAPVVVRVP"
BASE COUNT          728 a          859 c          893 g          618 t
ORIGIN      
        1 atgctacgtt catcctgagt ccggcaaaac gagcacatat tcatgagtca atcgcaaaca
       61 gctttagcgc agtcgcctac tccgctcaca acactttccg aggacgaaac catgtttcgc
      121 gcgagcgtcc gcgaattcgc cgaaggcgaa ctccgcccgc gcgttgaaca catggacgaa
      181 accgcaaaac tcgatccagc catcatcaac cagtgttttc agctcgggtt gatgggcatc
      241 gagaccccgg aagagtacgg cggcgccggt gccaactttt tcagtgccat cctcgctgtc
      301 gaagaactct cgcgcgttga cgcttccgtc ggcgtattcg tcgacgtgca aaacacgctt
      361 gtaaacaacg cgctgattcg ttggggaaca aacgagcaga aacagaagta cctcacgcgc
      421 ctcgcaagcg aacacgtcgg cgcctacgcg ctcagcgaac caagctcggg ctcagacgct
      481 ttcgccatgc agacgcgcgc ggtggacaaa ggcgatcact acgtgctcaa cggtcgcaag
      541 ctctggatca cgaacggcat cgaagccgaa attttcgtac ttttcgccaa tgccaatccc
      601 gaagccggct acaaaggcat caccgctttc atcgtcgaga agagttcacc cggtttttcg
      661 atcggaaaaa aggagaacaa actcggcatc cgcgcctcgt caactgtcga attgatcctc
      721 gaagattgcg aggtgccgaa ggaaaacgtt ctcggcgaag ccggcaaggg ctacaagatc
      781 tcgatcgaga cgttgaacga aggacgtctc ggcataggcg cgcagatgat tggcatcgcc
      841 gccggcgcgt tggagagtgc gctggcctac accgccgaac gacaacagtt tggcaaatcg
      901 atcaatcagt ttcagggagt tcagtttcaa ctcgctgaaa tggccaccga actcgaggcg
      961 gcccggctca tggtttacaa cgccgcgcgc atgaaggatg cgggcatgaa cttcgtgaaa
     1021 gaagcagcga tggcgaagct ttattcgtca cgcgtggccg agcgcgtatc ttcaaaagcg
     1081 atcgaactct acggcggcta cggatacgtc aaggattatc cagtcgagaa atactggcgc
     1141 gactcgaaga ttggcgccat ctacgaaggc acttctaaca tgcaattgca gacgatcgcg
     1201 aagttgatta tcggtaaatg aacaatcgta tcgagctgtt tcagcagatg ttaaccaacg
     1261 atccgggaaa caccactgtg cttttcggac tggcgaagga atacgagaag gccggtagag
     1321 atcaggagtt gatcgagacg ttgaaccgtt accttgagat ggttgacgac gaaggaaacg
     1381 ctttcggcat gttggcgggc gcgtacgagc ggcttggcaa acgcgacgag gcgcgcaaag
     1441 cttacgagcg tggtatcgag gctgcgcaac gccatggcca tccgggcatg gccgaagaat
     1501 accgcatgac gctggcgggt aactatgaac actgacacca aagttttgaa gctggtacgc
     1561 aaggctgatt caaccgcaga agctgtagac aattctgctg tgcagccggc gccgcgagca
     1621 gttccggctt ccgtttccaa agaagagcaa ttgccggatc acaaactgct cctcgagatg
     1681 taccgcacga tgtacctctc gcggcgcatc gacgacaaag agatccagtt gaaaggtcaa
     1741 aaccggatct tctttcagat cagcggtgca ggacacgaag ccgtgctggc tgcggcaggt
     1801 gtggtgttga agccgagtta cgactggttt tatccgtatt accgcgaccg tgcgctctgc
     1861 ctgcaacttg gcatgacgcc cgccgagcag ttgctttcct cagtcggagc ggaggcggat
     1921 ccgaactcgc acggccgtca aatgccgtct cactggggcc acaaaaaact gaacatcgtt
     1981 tcgcagtcgt cgccaaccgg aacgcaactg ctgcaagcag ttggttgtgc cgaagcgagt
     2041 tatcgtttcc acctcatacc cgaactcaag aagcggattg aagactttca cgacgatgaa
     2101 gttgtttacg tttcactcgg cgaaggcacc acgagcgagg gcgagttctg ggaagcgctc
     2161 aataccgcgt gcaatctgag tttgccggtt gtctttgtcg tggaggacaa cggttacgcg
     2221 atcagtgttc ccgtcgaagt gcaaactgcc ggcggcgacg tttcgcgact agtcgagggt
     2281 ttcccgtcgc tttacattca gcgctgcgac ggcaccgacg cgattgagtc gttggaagtc
     2341 atgcgtcgcg ccgttgacta ctgccgccag cgcaagggtc cctcgctggt ccatgcgaaa
     2401 gtcatccgtc cctattccca ctcgttgtct gacgacgaaa agctctaccg ccccgacgag
     2461 gagcgcgcga gcgacgccga gcgcgatccc gtgaaacgtt tcggcgcgtt gctgatcgat
     2521 tcaggcgtga tcgaccagca aggtttgcag cgaatcaaag acgaagcgga tcgtgaagtg
     2581 aacgaagccg ctgacatcgc gctcgcaagt gttcagcccg cgccggacac cgcaatgaag
     2641 tatgtttttt cgcctgacgt ggatccaacg tcgaaggagt ttgacactga agaccaggtg
     2701 caactctccg gcaatcccgg cacgatggtc gacttgatca atcgttgcct gcacacggag
     2761 atggcccgcg atccgcgcat cgtcgtgttt ggcgaagacg tggctgattg ttcgcgcgag
     2821 aaatatctcg gcgcggtgaa aggcaaaggc ggtgttttca aagtcacggc caatctgcaa
     2881 cgaaacttcg gcggcgctcg cgtcttcaac tcaccgctag cggaagcaaa cattgtcggc
     2941 cgcgcgatcg gcatggcgac gcgtgggctg aagccggtcg ttgagattca gttcttcgac
     3001 tacatctggc ccgcgatgca ccagatccgc aacgagctgg cgttgatgcg ttggcgttca
     3061 gcaaatgatt ggaaagcgcc ggtcgtggtg cgtgtgcc
//
LOCUS       DASTEY010000673         7851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1218475, whole genome shotgun sequence.
ACCESSION   DASTEY010000673 DASTEY010000000
VERSION     DASTEY010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218475"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VFO72_11690"
     CDS             <1..1196
                     /locus_tag="VFO72_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9480003.1"
                     /translation="EAIDFCDFYAREMLRYAGEQPLYQVAGEESQLEYIPLGVGAVIP
                     PWNFPLAILAGMTVASVVTGNTVVLKPSSDAPTIAYKFFEILEEAGLPPGVVNFMTGS
                     GAEVGDVIVDHQKTRYIAFTGSKEVGLRINERAAKVHEGQIWIKRVVAEMGGKDAIIV
                     ANDANLDDAATGVVQSAFGFQGQKCSACSRAIIDAQVYDQMIEKIADRTAKLKLAEPT
                     DPSTNLSAVINEKAFKTINFYIEKGKSEGGRVVVGGGSDGEQGFFIEPTVIADVKPGD
                     TIEQEEIFGPVLAVIKAENYDDALKIANDTQYGLTGAVYSADEAKLHRARKEFHVGNL
                     YLNRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLLLFLQAKVAAEKVGAATGRKASAA
                     ERTVI"
     gene            1214..2149
                     /gene="fmt"
                     /locus_tag="VFO72_11695"
     CDS             1214..2149
                     /gene="fmt"
                     /locus_tag="VFO72_11695"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET9480004.1"
                     /translation="MRVVFMGTPQAAVPTLRRCLEDGHEVVAVWTQPDRPSGRGNKIS
                     PSPVKEFALSHGLNVHQPQRIKTEEAKNLFASHDADVAVVVAYGRILPEEFLRAPARG
                     CINVHFSLLPKYRGAAPANWAIINGEKETGVTTMFLEPTLDTGPILLQRVTKIGETET
                     APELMQRLSEIGADLLSETLSRFDELTPQTQDHSQATFAPILRKEDGLIDWYWPAKEI
                     EQRIRGLQPWPNAYTVFRSRGLTIWEAQALLTISPGVYPGEVVVALRDELVVQCGKQT
                     ALRVLEVQPEARKRLAARDFINGMRVKIGDRFGQD"
     gene            2136..3437
                     /gene="rsmB"
                     /locus_tag="VFO72_11700"
     CDS             2136..3437
                     /gene="rsmB"
                     /locus_tag="VFO72_11700"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET9480005.1"
                     /translation="MGRIKQISPARGDAFEILREVEAGGFSSILLAADRRELKPVDRA
                     LMHELVLGVLRWQLWLDKTIEHFAKRSIDNLDLPVRLALRLGLYQLRFLTRIPASAAV
                     NESVNLVRSARVSSAAAFVNAVLRRSIREADYNPASRLSDPVEKLAIETSHPVWLIER
                     WSNSFGLEETEAFARANNMVPPVAFRLVNTDFTDILDRLRTAGGSIEPSKVAEDAWRV
                     SGASSVVRKLANEGRIYLQDEASQLVAQTVNLKPAEHVLDLCAAPGGKTTLMAKRAAN
                     SALIVAADRSARRLETVTKSSALHNLNSIKSVVLDAGNQLPFADSIFDRILVDAPCSG
                     TGTLRRNPEIRWRISEDDIRVLAAQQKAFLHQAARVLKPGGQLVYSTCSVERDENEDV
                     IAAFLESHRDFQQINTIRTWPHREGTDGFFIASFALSDLGC"
     gene            3448..4107
                     /locus_tag="VFO72_11705"
     CDS             3448..4107
                     /locus_tag="VFO72_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9480006.1"
                     /translation="MSLVARASSIFRSVAIVIAIGIVFLFGVATTVYLSLRSPEVKVP
                     DVVGKDRFEAERILAEADLKFRVRASRPSNQAKPDTVLFQLPRAGEVVKTGQTVAMDV
                     SRPTKEGESPEIVTPPENTNDNRNANSSNDNRPRRPRPRNINENANGNANGNANRAAN
                     ANRTNANTIDNANVPRANVNRSSTGNANLRPGSNLNDNRRPVAPRPTPIPNPTRTRPS
                     P"
     gene            4135..4812
                     /gene="rpe"
                     /locus_tag="VFO72_11710"
     CDS             4135..4812
                     /gene="rpe"
                     /locus_tag="VFO72_11710"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9480007.1"
                     /translation="MLNNRNIEIAPSILSANFACLGDQIEAVERGGASILHVDVMDGH
                     FVPNITVGLPVVKSLAKFTRLPIDAHLMISEPGIYAAQFVKAGAQMVSVHVEADPHLH
                     RTLMSIKSEGAQAGVVLNPSTPVSAVEEALQFVDYVLVMSVNPGFGGQEFIPQTLEKV
                     KRLRQMIDERQLSVRIEIDGGVDRSNIEKVVAAGAEIIVAGSAIFGKPDAEAAVRELR
                     NATVQWV"
     gene            4992..6521
                     /gene="bamD"
                     /locus_tag="VFO72_11715"
     CDS             4992..6521
                     /gene="bamD"
                     /locus_tag="VFO72_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET9480008.1"
                     /translation="MKSRVFILALFCAAVFGVPQTVFGQQKTGGQTELSTTQRLDVMS
                     SKLDSMRRSLNSALAAMPEDKDKKANADDPVVRLKSLEKEVASLTSEVYDIRNKNDKA
                     EKFDPAAVDRLEAAVAELNTRVDIGLQQTASARTSAAANASDKKKKKKGRFFGLFGGG
                     EDKYADLTGAAVAGRDRVLFEEAAKEVRKGRHDTGRLLFATIINTYPDSPFLPLAKLA
                     IADSFFLEGSTSSLIQAAQAYQDWLTFFPTDPLADDASLKVAEAEMRQMGLSDRDVSH
                     ARKAEQRLKVLLQQYPQSPLRPIVESRLAEVQENLAMHNLQIARFYYDVKFSTGKGGL
                     KGAQSRLKEIVDKYPCFSYNDEVFYRLASTYQAEEEPDEAARYYQKLLQEFPESEYIE
                     KAKDQLNIIGAPIPEKSTPNPCAKRAPQSFMGNLMQQVSGRANITVTKDGILISKDGK
                     QGTDLIDEALKYNGQLPARTTPEAPRTAPAVDPKTRNANSGAGPAASNSPTPAPAAIK
                     P"
     gene            6626..7129
                     /locus_tag="VFO72_11720"
     CDS             6626..7129
                     /locus_tag="VFO72_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480009.1"
                     /translation="MDPTSPEAQAELARRHKAAATTVAGLLIGVVLLSILTFVARPYL
                     VEKPPNWPLDYATRLLVLFFGLGAIVWRRNRFSAMRLRDIFGLSGVSGLIKTLEKTTI
                     QLALLAAAIAVIGFIVTVIQGNELYTYWSCAIAAVVLIYCYPTKSSWLKTVSYFAEPL
                     EEETLAE"
     gene            7201..7767
                     /locus_tag="VFO72_11725"
     CDS             7201..7767
                     /locus_tag="VFO72_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9480010.1"
                     /translation="MTRSLRTSLLALFGLLFCCPAILGQEAESESVARLLTRFTPATT
                     SVSSNASPTFEQASSIERRAFEQTNQVRAQNGLPSLEWDADICRMARSHSESMFRLNY
                     FSHVTPDGLRLRDRARAAGVLSFRVLAENIAYNQGYEDPGAFAVERWMASPKHLANIL
                     STEFRAMAIGTYVAPDGSVFLTQTFITR"
BASE COUNT         1761 a         2186 c         2248 g         1656 t
ORIGIN      
        1 ctgaagcgat tgacttctgc gatttctacg cgcgcgagat gttacgttac gccggcgagc
       61 agccgctgta tcaagttgcc ggtgaagaga gccaactcga atacattccg ctcggtgtcg
      121 gcgcggtgat tccgccgtgg aacttcccgc tggcgattct ggcgggcatg actgtcgcgt
      181 cagtcgtcac cggcaacact gtggtgttga aaccttcttc cgacgcaccg acgatcgcat
      241 acaagttttt tgagattctg gaagaggcag gtttgccgcc tggtgtcgtg aacttcatga
      301 ccggttcggg tgctgaagta ggcgacgtga tcgtggacca ccagaagact cgttacatcg
      361 cgttcaccgg ttcgaaggag gttggactca ggatcaatga gcgcgccgcg aaagttcacg
      421 aaggacagat ttggatcaaa cgcgtagtgg cggagatggg cggcaaggac gcgatcatcg
      481 ttgcgaacga tgcgaacctc gacgacgctg cgaccggcgt ggtgcagtcg gcgtttggtt
      541 ttcagggaca gaagtgttcc gcctgttcga gggccattat tgacgcccag gtctacgatc
      601 aaatgattga aaagatcgcc gaccgtacgg cgaaactgaa acttgcggaa cccacggatc
      661 cttcaacgaa cctcagcgcg gtgattaatg aaaaagcatt caagaccatc aacttctaca
      721 tcgaaaaggg aaagagtgag ggtggacgcg tcgtagttgg cggcggatct gacggcgagc
      781 agggcttctt tatcgagccg accgtcatcg ccgacgtgaa gccgggcgac acgatcgaac
      841 aggaagagat ctttgggcca gttctcgcgg tgatcaaggc cgagaattac gacgacgcgc
      901 tgaagatcgc taacgacacg cagtatggct tgacgggtgc ggtctacagc gctgacgaag
      961 cgaaacttca tcgcgcgcgc aaggagtttc acgtcggaaa cctgtatttg aatcgcaagt
     1021 gcaccggcgc gctggtcggc gtgcatccgt tcggcggatt caacatgtca ggcacggatt
     1081 cgaaggccgg tggccgcgat tacctgttgc tgtttctgca agcgaaagtt gccgctgaaa
     1141 aggtcggcgc cgctacgggc cgcaaagctt ccgccgctga gagaacggtt atttagtcgc
     1201 tcgatatcga cgcatgcgag tagttttcat gggaacacct caggccgctg ttcccacgct
     1261 gcgccgctgt cttgaggatg gtcacgaagt cgtagcggtg tggacgcaac cggatcgccc
     1321 gtcgggtcgt ggcaacaaaa tctcgccctc gccggttaaa gagtttgcgc tgtcgcacgg
     1381 tctcaacgtt caccaacccc aacgcataaa gaccgaagaa gcaaaaaatc tatttgcttc
     1441 gcacgacgcc gacgtcgctg tcgtcgtcgc ttacggccgt atcctgcctg aagaatttct
     1501 gcgcgcgccg gctcgaggat gtatcaacgt ccacttttcg ttgctgccca agtatcgtgg
     1561 cgccgcgccc gccaactggg ccatcatcaa cggcgaaaaa gagacgggcg tcacgaccat
     1621 gtttctcgaa cctacactcg acactgggcc aatactgctc cagcgggtga cgaagatcgg
     1681 tgagactgaa acagcgccgg agttgatgca gaggctttcg gaaatcggcg ccgatctgtt
     1741 gagcgaaacg ctttcgcgtt tcgacgaact cacgccgcaa acacaggacc acagccaggc
     1801 gacgttcgcg ccgatactga ggaaggaaga cggtttgatt gactggtatt ggccggctaa
     1861 ggaaattgaa caacgcattc gtggccttca gccgtggcca aacgcgtata cggtgttccg
     1921 ttcgcgtggc ctgacaatct gggaagcaca ggcattgtta accatctcgc cgggtgttta
     1981 tccgggcgaa gtagtcgtcg cgctgcgaga cgaactcgtg gtccagtgtg gtaaacagac
     2041 ggcgctgcgc gtgcttgaag ttcagcccga agcgcgaaag cggctggctg cgcgcgactt
     2101 catcaacggc atgcgcgtga agattggcga tcgttttggg caggattaaa cagatttcac
     2161 ccgcgcgagg cgacgcgttt gaaatacttc gcgaagtcga agcaggcggc ttttcctcga
     2221 ttctcctcgc agccgatcga cgcgaactca aacccgtgga tcgagctttg atgcacgagc
     2281 tggtgcttgg cgtgctgcgc tggcaattgt ggctcgataa aacgattgag cactttgcga
     2341 aacgctcgat tgataacctg gatttaccgg ttcgcctcgc gctccggctc ggtctttacc
     2401 agcttcgctt tctgacgcgt attccggcat cggcggcagt gaacgagtct gtgaatctgg
     2461 ttcggagtgc gcgcgtgagt tctgcggctg cgtttgttaa cgccgtgttg cgtcgctcga
     2521 ttcgtgaagc tgattacaat cctgcctcgc gactctccga tccagtagag aagcttgcta
     2581 tcgagacctc gcatccggtt tggctcatcg aacgttggtc caattcgttt ggactcgagg
     2641 agacggaagc gtttgcgcgc gctaataaca tggtgccgcc ggttgcgttt cgccttgtta
     2701 acacggattt cacggacatt ctggatcgac tccgcactgc gggaggatct attgaacctt
     2761 ctaaggttgc tgaagacgcg tggcgtgttt ccggcgcgtc atcagtagtg cgcaaactcg
     2821 caaatgaggg tcgcatctat ctccaggacg aagcctcaca actcgtcgcg cagaccgtca
     2881 atttaaagcc cgccgaacac gtgctcgatc tgtgtgccgc tccgggtggg aagacgaccc
     2941 tgatggcgaa gcgcgccgcc aacagcgccc tgatcgttgc tgcagatcgt tcggcgagac
     3001 gcctcgagac ggtcacaaaa tccagcgcgc tgcacaatct caactcgatc aaatctgtgg
     3061 ttctggatgc tggcaatcaa ctacctttcg cagatagcat ctttgatcgc attctcgtcg
     3121 atgcgccctg ctccggaacg ggcacgctcc ggcgcaatcc agagattcgc tggcgtattt
     3181 ctgaagacga catccgcgtg ctcgcggcgc aacagaaagc attcctccac caggccgcgc
     3241 gtgtcttgaa acccggtggt caactcgtct attcaacctg ctcggtcgaa cgcgacgaaa
     3301 acgaggatgt gatcgccgcc tttcttgaaa gtcacaggga ttttcagcag ataaacacca
     3361 tccgtacctg gcctcatcgc gaaggcacgg acggcttttt tatcgccagc tttgctctgt
     3421 ccgacctggg gtgctagact tcggggcttg agtttagttg ccagagcttc atccatattc
     3481 cgaagtgtcg cgatcgtaat cgcgattggg atcgttttcc tcttcggagt tgcgacaact
     3541 gtttatctgt ctttacgcag tccggaagtg aaggtgcccg acgtcgtcgg caaagatcga
     3601 ttcgaagcgg aacgaattct ggcggaggct gatctcaagt ttcgcgtacg cgcctcgcgg
     3661 cccagtaatc aggcgaagcc cgacacagtt ttgtttcaac taccgcgagc gggcgaagtc
     3721 gtaaagaccg gtcaaactgt cgcgatggac gtcagccggc caacaaagga aggcgagtcg
     3781 ccggagatag tcacaccgcc cgaaaataca aacgacaatc gaaacgcaaa ctcgtcaaac
     3841 gacaacagac ccaggcggcc ccgacccagg aacataaacg aaaacgctaa cggcaacgcg
     3901 aacggcaatg ctaaccgagc ggccaatgcg aaccggacaa acgcaaacac gatcgacaac
     3961 gccaacgtgc cgcgcgcaaa tgtgaatcgc agcagcacgg gaaacgcgaa cttgcgaccc
     4021 ggcagtaatc tgaacgacaa caggcgacca gtggctcctc ggcccacgcc aattcccaac
     4081 ccgaccagga cacgaccgag cccgtaattt agccggcagt gccgaggcga gtggatgctc
     4141 aacaacagga atatcgaaat cgccccatcg attttgtcgg ccaactttgc gtgcctgggc
     4201 gatcaaatcg aagcggttga acgcggcggc gcgagcattc tgcacgtcga cgtgatggac
     4261 gggcactttg tccccaatat cacggtgggc ctgccagtgg tgaagtcgtt ggccaagttc
     4321 acgcggttgc cgattgacgc ccatctgatg atttcggaac ctgggattta cgcggcccag
     4381 ttcgttaaag ccggcgcgca gatggtttca gtgcacgttg aagcagaccc gcacctgcac
     4441 cggacgctga tgtcgattaa gtcggaggga gcgcaggcgg gtgtggtgtt gaatccgtca
     4501 acacccgtgt ccgcggttga agaagctttg cagttcgttg attatgtgct cgtcatgtcc
     4561 gtcaatcccg gcttcggcgg tcaggagttc atcccacaga cgcttgagaa ggtgaaacgt
     4621 ttgcggcaga tgattgacga gcggcagttg agcgtgcgca tcgaaatcga tggcggcgta
     4681 gatcgttcaa atatcgaaaa agtggtagca gcgggagcgg agattatcgt cgcgggctcc
     4741 gccattttcg gcaaaccgga cgcggaagcc gcggtcaggg aactacgtaa tgccacggtc
     4801 cagtgggtct agggattgtc gattttcgat tgccgattgc cgatttgatg gttcgtgaaa
     4861 tcggcaattg gcaattgcaa ttggcaattc ggcggggccg cggcaaaccg aacgagagaa
     4921 aattgaatcc gataccccgg aactctcttt tctcgcaaga aaacaagttt ttattgctgg
     4981 aggtcgattt tatgaagtct cgtgttttta tcctggcgct tttctgcgcc gcggtttttg
     5041 gcgtgccgca gactgtattc ggccaacaaa aaaccggcgg ccagacagaa ctttcaacta
     5101 ctcagcgcct ggacgtgatg agctccaaac tcgactcaat gcgccgttcg ttgaatagcg
     5161 cgctcgcggc aatgcctgag gacaaggaca agaaggcaaa cgccgacgat ccggtcgttc
     5221 gactcaagag tctcgagaaa gaagttgcgt cactcacttc cgaggtctac gacattcgca
     5281 acaaaaacga taaagccgag aagttcgatc ccgctgcggt cgatcgactg gaagctgctg
     5341 ttgccgagtt gaacactcgt gtcgacatcg gactgcaaca gacggccagt gcccgcacca
     5401 gcgccgccgc gaatgccagt gacaaaaaga agaagaagaa aggacgcttc ttcggcctgt
     5461 tcggtggcgg tgaagataag tatgccgacc tgaccggagc tgccgttgcc ggccgcgatc
     5521 gcgtgctctt tgaagaggcc gcgaaggaag ttcgcaaggg tcgtcacgac accggccgtc
     5581 tgctattcgc cacgattatc aacacctatc cggattcgcc gtttcttccg ttggcaaaac
     5641 tcgctatcgc cgactcgttc tttctcgaag gttcaaccag ctcgttgatt caggcggcgc
     5701 aagcttacca ggattggctc acgttctttc cgaccgatcc actggcggac gatgcgagtt
     5761 tgaaagtcgc ggaagcagag atgagacaga tgggcttgtc ggatcgcgac gtttcccacg
     5821 cccgcaaggc agagcagcga ctgaaagtgc tgttgcagca gtatccgcag agtcctttgc
     5881 gaccgatagt tgagtcgcga ttggcggaag tgcaggagaa cctcgcgatg cacaacctcc
     5941 agatcgctcg gttctactac gacgtgaagt tttccaccgg aaaaggcggc ttgaaaggcg
     6001 cgcagtcgcg gttgaaagag atcgtcgaca agtatccgtg cttctcgtac aacgacgagg
     6061 ttttctaccg tctcgcctcg acctaccagg ccgaagaaga accagacgag gccgcgcggt
     6121 attaccagaa actcttgcag gaatttcccg agagcgagta catcgagaag gcgaaggatc
     6181 agttgaacat tatcggcgcg cccattccgg aaaagtcgac gccgaaccca tgcgcgaaac
     6241 gcgcgccgca gagttttatg ggaaacctga tgcaacaggt gtccggccgg gccaacatta
     6301 ccgtcaccaa agacgggatt ctgatcagca aggacggcaa acaaggcacc gacttgatcg
     6361 acgaagcttt gaagtacaac ggccagttgc ccgcgagaac gactcctgag gcgccaagaa
     6421 ctgcaccggc ggtggatccc aagactcgga acgccaacag cggcgccggg ccggctgcgt
     6481 caaatagccc gacgccggct cccgcggcga tcaagccgta ggccggctca gtgtctccgt
     6541 gcctctgtgg ttaatgatct cccgggaaca accacaaagg cactgagaca ctgaggtcgc
     6601 actcattcgc aaaggctaat cagctttgga tcctacttcc ccagaagccc aggcagagtt
     6661 ggcccgcagg cacaaagcag cagccaccac cgttgccggg ttgctgattg gagtggtcct
     6721 gttgtcgata ctgacctttg tggcccgtcc ctatctcgtc gagaaaccgc caaactggcc
     6781 cctcgattac gcaacccgtc ttttagtcct gtttttcggt ctcggcgcaa ttgtctggcg
     6841 ccggaaccgg ttctccgcca tgcgccttcg cgacatattc ggcctctcgg gtgtgtccgg
     6901 cctcatcaaa accctcgaaa aaaccaccat acagctagct ctcctggcag cggcaattgc
     6961 ggtgatcggc tttatcgtca cggtaattca gggaaacgag ctctatacgt attggtcgtg
     7021 cgccatcgcc gcggtagtgc tgatttactg ctatccgacg aagtcctcgt ggctcaagac
     7081 ggtttcctac ttcgccgaac ctctcgaaga agaaacactc gccgaataac aacctcaggc
     7141 aaaaacctgt tgatccaagt ttggccagtt tgttactctg gctaacgggt taaggggtaa
     7201 atgacacgca gcttgcgcac atccctgctc gccctctttg ggctcctgtt ctgctgtccc
     7261 gccattctcg ggcaggaggc cgagtcggag tccgtcgcca gactcctaac gcgctttacc
     7321 cccgctacga catccgttag ctccaatgcc tctcctactt ttgaacaagc cagcagtatc
     7381 gagcgacgag cgtttgaaca gaccaaccag gttcgtgcgc agaatggtct gccgtcactc
     7441 gaatgggacg ccgacatttg ccgaatggcg cgaagccatt ccgagagcat gtttcgtctt
     7501 aattactttt ctcacgtgac gccggacggt ctgcgtttgc gagacagagc acgcgccgcc
     7561 ggcgtactca gcttcagagt gctcgccgaa aacatcgcgt ataatcaggg ctacgaagat
     7621 cccggagcgt ttgcggttga gaggtggatg gcctcgccca aacatctcgc taacattttg
     7681 tcgacggagt ttcgcgcgat ggcgattggg acctatgttg ctccggatgg atcggtgttt
     7741 ctgacccaaa cttttattac gcgataattt gctgaagctt cctgccttcg ccaagatcaa
     7801 ctggactctg cgcatactgg gaaagcggcg tgatggttat cacgagatcg c
//
LOCUS       DASTEY010000674         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_625369, whole genome shotgun sequence.
ACCESSION   DASTEY010000674 DASTEY010000000
VERSION     DASTEY010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625369"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFO72_11730"
     CDS             complement(<1..677)
                     /locus_tag="VFO72_11730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET9480011.1"
                     /translation="MQSGSLSLAGKTAIVTGGSRGIGRAVVELFSSLAAKVVVNYLRD
                     EQAATATVAWARELGAEAVAIQADVSQLSDAERLVDETLERFGRIDFLICNAGIWEGG
                     PVESISEELWDRTIDVNLKGTWSVCRAAVPVMKRQKFGRIVIVSSTAGQRGEANVSNY
                     AASKGGQISFTKSLSSELASFGINVNCVAPGWVETEMTSDVSADTAQLESVIKTIPRG
                     RMATPEE"
     gene            complement(712..1464)
                     /locus_tag="VFO72_11735"
     CDS             complement(712..1464)
                     /locus_tag="VFO72_11735"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET9480012.1"
                     /translation="MDKRPLIGIPMRIELPTDRFYLSRHYSEAVEAAGGAPVHISLIP
                     NADYVDTVVDGLDGILLPGSHSDVDPLRYGQQPHPKLGEVHPVKDQTDLLIIEAAERK
                     GIPLFAICFGMQILNVARGGTLIQDIDSQLPEAIKHEQGVPRDRPSHGVTLSENTRLS
                     NMAGAESFAVNSHHHQAIEALGADLIATAWSSDGIIEALEDPRADRFVVAVQWHPELG
                     WERDVMSQRLFKVFVAEASKLAEAAGAVVSIS"
     gene            1547..2092
                     /gene="hslV"
                     /locus_tag="VFO72_11740"
     CDS             1547..2092
                     /gene="hslV"
                     /locus_tag="VFO72_11740"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET9480013.1"
                     /translation="MRKESNKVRSTTVLLVRKDTRVALAGDGQVTIGETVMKASAKKV
                     RRLYNDQILAGFAGATGDAFALLTRFESKLEQYHGNLERAAIELSKEWRTDKMLRQLE
                     SMLVVADKKGSFLLSGNGDVIAPDEGVLAIGSGGSYALAAARALIKHTDLSAREIAVE
                     ALRIAGEICIYSNQNIVVEEL"
     gene            2107..>2345
                     /locus_tag="VFO72_11745"
     CDS             2107..>2345
                     /locus_tag="VFO72_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480014.1"
                     /translation="MVIYLAGEVDEDARLDEMSPRQIVTELDKHVVGQRNAKRAVAIA
                     LRNRVRRQKLAPEMAVDVMPKNIIMIGSTGVGKTE"
BASE COUNT          537 a          678 c          616 g          514 t
ORIGIN      
        1 atctcttccg gcgtcgccat tcggcctctt gggatcgttt tgataaccga ttcgagttgc
       61 gcggtatcgg ccgacacgtc gctggtcatc tcggtctcga cccatcccgg cgcgacgcag
      121 ttaacattga tgccgaaaga cgccagctcg ctggacagcg acttcgtaaa agaaatttgc
      181 ccgcccttcg aagccgcgta gttcgaaacg ttcgcttcgc cacgctggcc ggccgtagaa
      241 ctcacgatta ctatgcgccc gaacttctgt cgtttcatta ccggaacggc cgcccggcaa
      301 accgaccagg tcccttttag attgacgtct attgttcgat cccaaagctc ttctgaaatc
      361 gactcgactg gaccaccttc ccaaatcccg gcattgcaaa tcagaaagtc gatacgcccg
      421 aaacgctcca gtgtttcatc aacgagccgc tcggcgtcac tcaattgcga aacgtccgcc
      481 tggatcgcga ctgcctcagc tccgagttct ctggcccagg cgacggttgc ggtcgcagcc
      541 tgctcgtctc gaaggtagtt cacaaccacc ttcgccgcga gtgacgagaa cagttcgaca
      601 acggctcttc cgatgccgcg cgatccgccc gttacgattg ctgtttttcc tgctaagctc
      661 aggctaccgc tctgcactgc gattccagtt cccttcccgt atggatgccg ctcaggagat
      721 ggagacaaca gcgcccgctg cttctgctag tttcgacgct tccgccacga agactttgaa
      781 caatcgttgc gacatcacat ctcgttccca gccgagttca ggatgccatt gcaccgcaac
      841 tacaaatcga tccgcacgcg gatcttcgag agcttcaata ataccgtccg atgaccacgc
      901 cgtcgcaatc aggtctgcgc caagtgcttc gattgcctga tggtgatgac tgtttactgc
      961 gaaactctcc gcgccggcca tattcgacaa tctggtgttc tcagagagtg tgacgccgtg
     1021 cgacgggcga tcgcgaggca caccctgctc atgtttgatc gcctcaggca actgcgaatc
     1081 gatgtcttgg atcagcgttc cgccgcgggc cacgttcaag atctgcattc cgaaacagat
     1141 cgcgaacagc ggaatccctt ttcgctcagc agcttcaatt atcaacagat cggtttgatc
     1201 tttaaccgga tgtacttcac ccagctttgg atgcggctgc tggccgtaac gtaacggatc
     1261 gacgtcggag tgactgcccg gcagcagaat gccatcgagt ccatcaacga ccgtgtcaac
     1321 gtagtccgcg tttggaatca agctgatgtg caccggagcg ccacccgctg cttcaacagc
     1381 ttcactgtaa tgtcgcgaga gataaaagcg gtctgttggg agttcaatac gcatcggtat
     1441 cccgatgagg ggtcgtttgt ccatgcggca atgataacct atgtccctcg aaatgctctc
     1501 cttgacttac gttcaagcca gtttctatag tgctcactaa agacgcatgc gcaaagaaag
     1561 caataaagtg cgcagcacaa ccgtgctgct cgtgagaaaa gacactcgtg tggctctcgc
     1621 cggcgacgga caggtgacaa tcggcgagac ggtgatgaag gccagcgcca agaaggtacg
     1681 ccgcctttat aacgaccaaa tcctggccgg ttttgccggt gccaccggcg atgcgttcgc
     1741 gctactaacc cgtttcgaaa gcaaactcga gcaatatcac ggcaacctcg aacgcgccgc
     1801 aatcgagctg agcaaagaat ggcgcaccga caagatgctg cgtcaattgg aatcgatgct
     1861 ggttgttgcc gacaagaaag gatcgtttct cttgtcgggc aatggtgatg tcatcgcgcc
     1921 ggacgaaggc gtgctggcga tcgggtcagg cggatcttat gctctagcgg ccgcacgtgc
     1981 gttgattaaa cacacggatc tttcggcgcg cgagatcgcg gtagaagcac tgcgcatcgc
     2041 cggagaaatc tgcatctatt caaatcaaaa catcgtagtt gaggaactgt aatcagtttt
     2101 aggcaaatgg ttatttacct ggcaggagaa gttgatgagg acgcccggct cgacgaaatg
     2161 tcgcctcgtc aaatcgttac tgaactcgac aagcacgtcg tcggacaacg caacgcgaaa
     2221 cgcgccgtag cgatcgcttt gcgaaatcga gtgcgacggc agaaattggc gcccgaaatg
     2281 gcagtcgacg tgatgccaaa aaacatcatc atgatcggct caacgggtgt cggcaaaact
     2341 gagat
//
LOCUS       DASTEY010000675         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1597588, whole genome shotgun sequence.
ACCESSION   DASTEY010000675 DASTEY010000000
VERSION     DASTEY010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597588"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="nadA"
                     /locus_tag="VFO72_11750"
     CDS             <1..1066
                     /gene="nadA"
                     /locus_tag="VFO72_11750"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET9480015.1"
                     /translation="MIEMFTDAATEEMYQKLKRLLADTVPDFELRLKAELAAKINRLK
                     AEKNAVILGHNYMEPALFHSIPDFVGDSLELSRRAAETDKDIIVFCGVKFMAETAKIL
                     NPTKKVLIPSEKAGCSLAASITAEDVRELKKRFPGVPVVTYINTYADVKAESDICCTS
                     SNAVDVVNSLGTDKVIFIPDEYLASNVARETGKQIIFPTREPRHQFPRELDYEMIGWP
                     GRCEVHEKFSVEDIRNIRKQFPDVVILAHPECSPEVVAESDYSGSTTAMMRYVEKTRA
                     PRYLLLTECSMGDNIAAANPDKEMLRLCSVRCPHMNQITLEDTLESLRKEQYEIVIDE
                     DIRKRALRSVERMLEIGRED"
     gene            1066..2673
                     /gene="nadB"
                     /locus_tag="VFO72_11755"
     CDS             1066..2673
                     /gene="nadB"
                     /locus_tag="VFO72_11755"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET9480016.1"
                     /translation="MAESEVLIIGCGIAGATAALRLARNPNRRITIITRAHDTHESNT
                     RYAQGGIIGRGPDDNAELLFEDIIAAGAGVSSPEAARILAEEGPPLLNEILEKTAGVV
                     FDRDEEGAPVWGQEAAHSRRRILHVGDATGLAIINGLIRTLNSHPNIRIESEATAVDL
                     ITFPHHSRDPLDNYREISCHGCYVFDREESTVHRYLARATVLATGGLGRIYRNTTNPV
                     GARGDGLAMAHRAGARIANAEYVQFHPTALAAPGAEGLLISEAVRGEGGVLLTPDLRP
                     FMHKYSPEWKELAPRDIVARAIHHEMEANGYSYVLLDIASHMPAEAIRDRFPFIYHEC
                     LKVGLDISREPVPVVPAAHYFCGGVLVDEWGRSTIDNLYAIGEISCTGVHGANRLAST
                     SLLEGLVWGNRVAIEIERALDEAGDEKQPKFNDIPPWDESGLSADPDPALIQGDMQTI
                     QNIMWHYVGLVRSADRLSRAIRELRHLWNEIETFYRTTKLSDGLIGLRNAVEIALIVA
                     QAAQHNRQSRGCHYRQDSVSFEGDRLI"
     gene            2822..3565
                     /locus_tag="VFO72_11760"
     CDS             2822..3565
                     /locus_tag="VFO72_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480017.1"
                     /translation="MPETLGPLSTFAVLGIGLIFGLKHATEVDHVVAVSTIVSQHRNV
                     WRSALVGALWGAGHTAALLITGFFVLSLRIAIPVRVSDWLEFCVALMIIGLGATALWR
                     ALRKRGDVHVHEHSHDGVAHVHVHFHDPQTRHNRRPSHEHGISRIGMKPVLIGALHGL
                     AGSGALTLLVLTQIESTWRGVLYLGVFGAGSIAGMLLMSGLIGLPFALTSGKLTTMHH
                     RLQTAAAVFSLAFGFWYAYELLEVTSFAP"
     gene            complement(3642..4694)
                     /locus_tag="VFO72_11765"
     CDS             complement(3642..4694)
                     /locus_tag="VFO72_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET9480018.1"
                     /translation="MPACLCGEDDRQECPTGGLEELLKLLVVDSQQITQLLPMAECIE
                     LMSDALASLARGEVYQPLRTIIRPPDARGLLGLMPAYRHGEHGAFGLKAICVFPGNPS
                     LGKDAHQGTVMLFSQETGEPLALMNASQITAIRTAAVSAVATRLLARPEAEELGIIGA
                     GVQARTHLLALACVRSIKRARVACRNNDHAMALVREMQDKVGFTIESAETNEAAVRGA
                     DLIVTATSAHEPVIKRDWIADGAHINAIGTHSPQSREIDSETMAAARIFVDRRESALN
                     EAGDYLLAAQEGAVTPESIVAEIGELVIQSKQGRAAPHEITLFKSLGLAIEDVVCAEY
                     LFRKAKAENTGTWVDF"
     gene            4871..>5149
                     /locus_tag="VFO72_11770"
     CDS             4871..>5149
                     /locus_tag="VFO72_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480019.1"
                     /translation="MKKGLISIVCLIVLTLSALPVGAQTRYRRYNDGRRYEQRYDGRR
                     NRNWDRDRSFWDRHRDKLTTAAGAGAGALIGAAVGGKKGAIIGAIAGGG"
BASE COUNT         1092 a         1433 c         1515 g         1109 t
ORIGIN      
        1 aatgattgaa atgtttactg acgccgcaac cgaagagatg taccagaagt tgaaacggct
       61 attggccgat accgtgcccg acttcgagtt gcgtctcaaa gctgagcttg cggcgaagat
      121 caaccgattg aaagcggaaa agaacgccgt gatactcggt cacaattaca tggagccggc
      181 gctctttcat tcgattcccg acttcgtcgg cgactcgctc gaactcagtc gccgagccgc
      241 ggaaaccgac aaggacatca tcgtcttttg cggcgtgaag tttatggccg agacagccaa
      301 gattctcaat ccgaccaaga aggtgctgat accttcggag aaagcaggct gctcgctggc
      361 cgcgagcatc actgccgaag acgtgcgcga gttgaagaaa cgttttcccg gcgttccggt
      421 cgtgacctac atcaacacct acgcggatgt gaaagctgag tctgacatct gctgcacgtc
      481 gtcgaatgcc gtcgacgtcg ttaactcatt aggcacggac aaagtcatct tcattcctga
      541 tgagtacctc gcgagcaacg tagcgcgaga aaccgggaag cagatcatct ttccgacgcg
      601 cgagccgcga caccagtttc ccagggaact ggactacgag atgattggct ggcccgggcg
      661 ctgcgaagtg cacgaaaagt tcagcgtcga ggatattcgc aacatcagga agcagtttcc
      721 ggacgtggtg atcctggccc atccggagtg cagtcccgaa gttgtagccg agtcagacta
      781 ttccggcagc accacggcga tgatgcgtta cgtggaaaag acgcgggcgc cgcgttatct
      841 actgctgacg gagtgctcga tgggcgacaa tatcgccgcc gcaaatcctg acaaagaaat
      901 gctgcgactg tgcagtgtgc gttgtccgca catgaatcag atcacgctcg aagacacgct
      961 cgagtcactg cgaaaagagc agtacgagat cgtgatcgac gaagacattc gcaagcgagc
     1021 gctgcgttcc gtcgagcgga tgctcgagat tggtcgtgag gactgatggc agaaagcgaa
     1081 gtgttaatca tcggttgcgg catagccggc gcaaccgctg ctcttcggct ggcgcgcaat
     1141 ccgaatcgcc gcataaccat cattactcgc gcgcacgata cccacgaatc caatacacgt
     1201 tatgcacaag gcggcatcat cggacgcgga cctgacgaca acgccgagtt gttgttcgaa
     1261 gacatcatcg ccgccggcgc gggtgtgagc tcgcccgagg ccgcgcgcat tctcgctgaa
     1321 gaaggaccgc cgctgctgaa tgaaatactc gaaaaaacag cgggagtcgt tttcgatcgc
     1381 gacgaggaag gcgcacccgt atggggacag gaagcagcac actcgcgtcg ccgcattttg
     1441 cacgttggcg acgcaacggg actcgcgatc attaatggtc ttattcgcac gcttaacagc
     1501 caccccaaca ttcggatcga gtcagaggcc actgctgtcg acctaatcac gtttccgcat
     1561 cactcgcgcg atccactcga caactatcgc gagatctcgt gtcatggttg ttacgtgttc
     1621 gatcgcgaag agagcactgt acatcgttac ctggcgcgtg cgacggtgct ggcgaccggt
     1681 ggtctcgggc ggatctatcg caacaccacg aatccggtgg gcgcgcgcgg cgatggtctc
     1741 gcaatggccc atcgcgccgg agcacgtatc gcaaacgcgg aatacgtgca gtttcatccg
     1801 actgcgctcg ctgcgccggg tgctgaaggt ctgttgatca gcgaggctgt gcgtggcgag
     1861 ggaggcgtgt tgctgacgcc ggatctgcgg ccgttcatgc acaagtattc gccggagtgg
     1921 aaagaactgg cgccacgcga catcgttgcg cgggcgattc atcacgagat ggaagcaaac
     1981 ggctactcgt acgtgctgct cgacatcgcc tcgcacatgc ccgccgaagc aattcgcgac
     2041 cgctttcctt ttatctatca cgaatgcttg aaggtcggtt tggacatctc acgcgaaccg
     2101 gtgccggtcg tgccagccgc gcattacttc tgcggcggcg tgttggtgga tgagtgggga
     2161 cgctccacga tcgacaacct ttatgcgatt ggcgagatca gttgtaccgg agtccacggt
     2221 gcaaatcgtc tcgcgtcgac ttcgctgctc gaaggtctcg tttggggaaa tcgcgtggcg
     2281 atcgaaattg aacgggcgct cgacgaggcg ggcgacgaga agcagccgaa attcaacgac
     2341 attccgccgt gggacgagag tgggttgagt gctgatccgg atccggcgct gattcagggc
     2401 gacatgcaga cgattcaaaa tataatgtgg cactatgtcg gactggtccg cagtgccgat
     2461 cggctgtcgc gagcgatccg cgaattgcgt cacttgtgga acgaaattga aacgttctat
     2521 cgtacgacga aattgtctga tgggttgatc ggactgcgca atgcagttga gatcgcactg
     2581 atcgtcgcgc aggcggcgca gcacaatcgg cagagccgtg gctgtcatta tcggcaagac
     2641 tctgtttcgt ttgaagggga tcggttgatt tgagtttgct gcatcgttcg cagcacgccg
     2701 tgacagaggg gagtctgttt cctgggactg caggcgtctc gcctgcaacg catcgcagca
     2761 ggcgtgatgc gaagcatagc aggcgagacg cctgcggtcc caccgtgtat agtacgcatt
     2821 tatgccggag acacttggcc cgttgagcac ctttgccgtg ctcggtatcg gtttaatatt
     2881 tggattgaag cacgcgacgg aagtggatca cgttgttgct gtttcgacta tagtcagcca
     2941 gcatcgcaac gtctggcgat ccgcgctcgt cggcgcgctg tggggtgcgg gccacacggc
     3001 tgcactgctg atcacgggat ttttcgtctt gtcgttgcga atcgcgattc ccgtgcgcgt
     3061 cagtgactgg ttggagtttt gtgtggcgct gatgattatc gggctgggcg ctacggcgct
     3121 gtggcgtgcg ctgcgcaaac gcggcgatgt tcacgttcac gagcacagcc acgacggtgt
     3181 tgcccacgtt cacgtccact ttcacgatcc gcaaacccgg cacaatagac gtccctcgca
     3241 cgagcatggc atctcacgca tcggtatgaa accggttttg atcggtgcac tgcatggact
     3301 cgccggctcg ggcgcgctca cgttactcgt gttgactcag atcgaatcca cctggcgggg
     3361 cgtcctgtac ttaggggtgt ttggcgcggg ttcgattgcc gggatgttgt tgatgtcagg
     3421 cttgatcgga ctgccgttcg cgttgacttc aggcaagctc acgacgatgc atcacaggct
     3481 gcaaaccgcc gcagcggttt ttagtctcgc gttcggcttc tggtacgcct acgagttact
     3541 cgaagtaact tcctttgcgc cttagcgcct ttgcgcgagt ttgttttctc acgctagggc
     3601 gcaaagccgc aaagatcttc cgtctcgcgt cgaggtctcc tttaaaagtc aacccaagtc
     3661 cccgtattct cggcttttgc tttgcgaaag aggtattccg cgcacacgac gtcttcgatc
     3721 gcgagtccga gagatttgaa gagcgttatc tcgtgcggcg cggctcgccc ttgtttgctc
     3781 tgaattacta actcaccgat ctcggcaacg atactctccg gagtgacggc gccttcttgt
     3841 gcggcaagca aataatcacc agcctcgttc aacgcagact cgcgacgatc gacaaagatg
     3901 cgtgcggcag ccatcgtctc actgtcgatc tcgcgcgact ggggtgagtg tgtaccgatc
     3961 gcattgatgt gtgcgccgtc agcaatccag tctcttttaa tcacgggctc gtgtgccgac
     4021 gtcgccgtca caatgagatc ggcgccgcga acagccgctt cgttggtttc tgctgattcg
     4081 attgtaaatc cgactttatc ctgcatctcg cgcacgagcg ccatcgcgtg atcgttattt
     4141 cgacacgcca cacgagcacg cttaatcgaa cgaacacagg cgagcgccaa cagatgagta
     4201 cgtgcctgca cgcccgctcc aatgattccc aactcctccg cttccggacg cgcgagcagt
     4261 cgcgtggcga cggctgaaac agcagcagta cgtatcgcag tgatctgcga cgcattcatc
     4321 aacgccagcg gctcgccggt ttcctgactg aacaacatca ccgtgccctg atgtgcgtct
     4381 tttccgagcg acgggttgcc gggaaacacg cagatcgcct tcaacccgaa tgcgccgtgt
     4441 tctccatgtc tgtacgcggg catcaacccc agcaggccgc gcgcgtcagg tggacgaatg
     4501 atagtgcgca acggttgata gacttcgccg cgagcgagcg acgccagcgc gtctgacatc
     4561 agctcgatgc actcagccat cggcagaagc tgagtaatct gttggctgtc aactactagt
     4621 agcttcagta gttcctccag tcctccagta ggacattctt gtctgtcgtc ttcgccgcag
     4681 agacaggcag gaatgcctgt cctacatacc ccaaaaggtc aatcacctca cagaatgcgc
     4741 acgttatggt taacgtcctg cgccaatttt ttaactcaac tcctgcaaaa ctgcaaaaaa
     4801 gcgcgcgtct ttggattggc acactcattg ccaaaagagg gagcgctagg cagtaggagg
     4861 agagcaagga atgaaaaagg gtcttatcag cattgtttgt ttgatagtgc tcacgctttc
     4921 ggcgctccca gttggagcgc agacgcggta ccggcgctac aacgacggtc gacgttatga
     4981 gcagcgctac gacggacgtc gcaatcgcaa ttgggatcgc gatcgatcgt tttgggatag
     5041 acatcgcgac aagttaacga ccgccgcggg ggcaggcgcc ggagctttga tcggcgcagc
     5101 ggttggcggc aagaaaggtg cgattatcgg agcgatagcc ggtggaggt
//
LOCUS       DASTEY010000676         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_987616, whole genome shotgun sequence.
ACCESSION   DASTEY010000676 DASTEY010000000
VERSION     DASTEY010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987616"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2127
                     /locus_tag="VFO72_11775"
     CDS             <1..>2127
                     /locus_tag="VFO72_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9480020.1"
                     /translation="RLGTHFQRLLDAIVSNPEQRLSDIQFLEETERRQVLSDWNATTV
                     DYPKDKCIQQLFEAQVEQTPHAIALVFEDRQLTYDELNSRANQLARHLRDLGVRNEML
                     VGLCVERSIEMIVGMLGILKAGAAFVPLDPSYPMDRLSFMFEDARLPVLLTQEHIAAS
                     LPCQWVQTICLDSDWDLIATQSEENLDVVSEPDNLAYVIYTSGSTGKPKGVMLQHTGL
                     GNMVLAQITAFGIRSDSRVLQFASFSFDASVSEVFMALLAGARLYLGTRETLMPGPAL
                     LQTLRENEITVVTLPPSVLAAMDGEQLPALEVIIAAGESCSLETVERWTKGKRFFDAY
                     GPTEDTVCATLAELDGSRVTIGGPIANNQIYILDKNLQPVPAGVFGELHIGGVGLARG
                     YLNRPDLTAEKFIANPFGDGSRIYKTGDLARWFPDGNIDFFGRIDNQVKVRGYRIEAG
                     EIEAVLNSHPEVSTSVVIAREDSAGEKRLAAYCIPRTIEREEFVSEGQMELWPSVAEF
                     YVYDDLLYLAMTADERRNESYKVAINRHVKDKVVLDIGTGKDAILARFCVEAGARRVY
                     AIELLKETYDKAKATIESLGLTDRIRLIHGNSMEVELPEKIDVCVSEIVGPIGGVEGA
                     APIINNAWRFMKDDGVMIPERSLTRIAAVTLPEEFLENPSFAKVAARYVGKVFEQAGY
                     KFDLRLCLRNFSKENIVSNYQPFEDL"
BASE COUNT          494 a          541 c          622 g          470 t
ORIGIN      
        1 atagattagg aacacacttt caacgactgc tggacgctat tgtttccaat cccgaacagc
       61 gtctttccga cattcagttt cttgaagaaa cagaacgccg gcaggtgctt agcgattgga
      121 acgcaacgac agttgactat cccaaagaca agtgcatcca gcaactgttt gaggcacaag
      181 tcgagcaaac tcctcacgcg atcgcgctcg tctttgaaga ccggcaactg acctatgacg
      241 agctgaactc acgcgcgaat caactcgccc gtcacttgcg agatctgggc gtccggaacg
      301 agatgctcgt cggtctttgt gtggagcgat cgatcgagat gatcgtgggc atgctcggta
      361 tcttgaaagc cggcgcggcc ttcgtacctt tggatccgag ttatccaatg gatcgtctct
      421 cgttcatgtt tgaagatgcg cggttaccgg tactgctgac gcaggagcat atcgccgcga
      481 gtttgccatg ccagtgggtg caaacgatct gtctggatag tgattgggat ctgatcgcga
      541 ctcaaagtga agagaacctc gacgtcgttt cagagcctga caatctcgct tatgtcattt
      601 acacctcagg ctcgaccgga aaaccgaaag gcgtaatgct ccagcacacc ggtctgggga
      661 acatggttct ggcgcaaatc acagcgtttg gcattcgtag cgatagtcgg gtgttgcagt
      721 tcgcatcgtt cagcttcgac gcatcagtgt cggaggtttt catggcgctg ctcgcgggcg
      781 cgagactcta tctcggaacg cgtgagacgc taatgccggg accggcgttg ctgcaaacat
      841 tacgcgaaaa cgaaatcaca gtcgtaacgt taccgccgtc tgtcctggct gccatggacg
      901 gcgagcaatt gcccgcactg gaagtgatca tcgcggccgg tgaaagctgt tcgttggaga
      961 cggtagagcg ctggactaaa gggaagcggt ttttcgatgc ctatggtcca accgaagata
     1021 ctgtttgcgc gacgctcgcc gagctcgacg gcagtcgtgt gaccattggc ggaccgatag
     1081 cgaacaacca gatctacatt ctcgataaga atcttcagcc ggtgcccgcg ggcgtttttg
     1141 gcgaactgca tatcggcggc gtcgggctgg cccgtggata cttgaatcga cctgatctga
     1201 ccgccgagaa gttcatcgcg aatccgtttg gcgatggtag tcgcatttac aagacgggcg
     1261 atctggcgcg ttggttcccc gacggcaata tcgatttctt cggtcgtatc gacaatcaag
     1321 tgaaagtacg cggctatcgc atcgaggccg gcgagatcga agcggtactc aacagtcatc
     1381 cggaagtgag cacgagcgtt gtgatcgctc gcgaagacag cgccggcgag aaacgattgg
     1441 ctgcttattg cataccgcga acgatcgaac gcgaggagtt tgtttcggaa ggtcagatgg
     1501 agttgtggcc ttcagtcgct gagttttatg tgtacgacga tctcctctat ctcgcgatga
     1561 ctgcggacga gcgccgcaac gagagttaca aagtcgcgat caaccggcac gtcaaagaca
     1621 aagtcgtact cgacatcggc accggtaagg atgcgattct cgcaaggttc tgcgtcgaag
     1681 caggcgcgag aagagtttac gcgatcgagc tcctgaaaga gacttacgac aaagcgaaag
     1741 caacgattga gagcctcggt ctgacggaca ggatcaggtt gattcatggc aactcgatgg
     1801 aagttgagct gccggaaaag atcgacgtct gcgtgtcgga aattgttgga ccgatcggcg
     1861 gcgttgaagg cgcggcgccg atcatcaata acgcctggcg cttcatgaaa gatgacggcg
     1921 tgatgattcc ggagcggagc ctcacgcgca tagccgccgt aactctcccg gaagagttcc
     1981 tggagaaccc gagcttcgcg aaagtcgcgg cacgctatgt tggcaaagtt tttgagcagg
     2041 cgggatacaa gtttgatctg cgtctttgtc tgcggaattt ctcaaaagag aacatagtct
     2101 ccaactatca gccgttcgag gatctcg
//
LOCUS       DASTEY010000677         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1581113, whole genome shotgun sequence.
ACCESSION   DASTEY010000677 DASTEY010000000
VERSION     DASTEY010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581113"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFO72_11780"
     CDS             <1..199
                     /locus_tag="VFO72_11780"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9480021.1"
                     /translation="SDAEALEAFQLLSKLEGIIPALESAHAVAHAIKVARELRSDQVL
                     IANLSGRGDKDINSVREQLES"
     gene            204..1016
                     /gene="trpA"
                     /locus_tag="VFO72_11785"
     CDS             204..1016
                     /gene="trpA"
                     /locus_tag="VFO72_11785"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET9480022.1"
                     /translation="MGRIEAAFSELKGRRKRGFIPFITAGDPDLETTAEILREFSGAG
                     ATLIELGVPFSDPMADGPVIQRSSERALKHGFGVGDLLEVVAGVRRTIETPIILFSYF
                     NPLLQFGLKRLAEQARAAGVDGVLVTDLSPEEAGEFDTELRANDLDMIFLIAPTSTDE
                     RLKLVAQHARGFIYAVSRAGITGMQTSVSAEAEKLVNRMRKFSSLPIAVGFGISNAEQ
                     VRDVHRYADAVVIGSAIVSEIERLQGATTDLPKKIGDFTRSLIPPYSCADLT"
     gene            1236..2288
                     /gene="recA"
                     /locus_tag="VFO72_11790"
     CDS             1236..2288
                     /gene="recA"
                     /locus_tag="VFO72_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9480023.1"
                     /translation="MADDRMTAERGRAIEAALANIEKKFGKGSIMRLGERDVSDIPAI
                     STTSLSLDAAIGVGGVPRGRIVEIYGPESSGKTTLALHIVAEAQKAGGVAAYIDAEHA
                     MDADYAGKLGVDIDQLLISQPDSGEQALEIAEALVRSNGVDVIVVDSVAALVPRAELD
                     GEMGDSLPGLQARLMSQALRKLTAIVAQSNTCFIFINQIREKIGVMFGNPETTTGGRA
                     LKFYSSLRLDIRRIGAIKDGDRVVGNRTKVKVAKNKVAPPFRECEFDIMYGEGISREG
                     DLLDLAVAQRIVEKSGAWFSFKGERLGQGRENAKQALRENAEVLKRIEREVKGKLGIP
                     VREEPAAPSNVKAASK"
     gene            2358..3686
                     /locus_tag="VFO72_11795"
     CDS             2358..3686
                     /locus_tag="VFO72_11795"
                     /inference="COORDINATES: protein motif:HMM:NF005557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HET9480024.1"
                     /translation="MSSILIKNGTLVTMDSANRIVRGDLLISGSHIVELGDISQSADV
                     VIDAGGCALIPGFVQTHIHLCQTLFRGAADDLALIDWLKKRVWPMEAAHSAASIAASA
                     RLGVAELIKGGTTCALTMETVNHTAKVFEVVEETGFRATVGKCMMDKGDEVPEALHEQ
                     TAESIEESIALLEQWHGKANGRIRYCFAPRFAISCTRELLEKVGELARVHSVMIHTHA
                     SENRTECALVEQETGLRNIVYLDSVGISGRHVALAHCVHLNNNEIELLKNTRTNVVHC
                     PSSNLKLGSGIAPIAKLLDEGISVSLGADGAACNNRLDMFTEMRTTALLQKALHGPEV
                     LAANRVLRMATIDGAKALGLEAEIGSLEVGKRADVAVVKLGQVHTAPEADVVSSLVYS
                     AVASDVQSVVIDGRMVMRDRELLTIDEAETVVEADAQAELLMTRAGLTTD"
BASE COUNT          806 a          934 c         1206 g          818 t
ORIGIN      
        1 gtcggacgcg gaagcgcttg aggcgtttca actgttgtcg aagctcgagg gaattattcc
       61 cgcgttggag tcggcacacg ccgtggcgca cgcgattaag gttgcgcgcg aactgcggtc
      121 ggatcaggtt ttgatcgcga atctttccgg gcgcggcgac aaagacataa actccgtccg
      181 cgaacagttg gagtcgtaaa agcttgggac gtatcgaagc agctttcagt gagttaaagg
      241 gacgacgtaa gagggggttt attccgttta ttactgcggg agatccggat ctcgagacga
      301 cggctgagat cttgagggag ttctcaggag cgggtgcaac cttgattgaa ctgggtgtgc
      361 cgtttagcga cccgatggcc gatgggccag tgattcagcg ttcgtcggaa agggcgctga
      421 agcacggctt tggcgtaggg gatttgctcg aagttgtcgc tggcgttcgg cgcacgattg
      481 aaacaccgat tattctgttt agctatttca acccgctgtt gcagtttggt ttgaagcgac
      541 ttgcggaaca agcgcgggcg gccggtgttg acggtgtgtt ggttacggat ctgagtcctg
      601 aagaagccgg cgagtttgac actgagttgc gcgcgaatga tctggacatg atctttctta
      661 ttgcgccgac ttcgaccgac gagcgtttga aactggtcgc gcaacacgca cgcgggttca
      721 tctacgctgt ctcacgcgcc ggcatcaccg gcatgcagac cagcgtcagc gctgaagccg
      781 agaaactcgt caaccgcatg cgaaagtttt cgtcgttgcc gatcgccgtg ggcttcggta
      841 tctcaaacgc tgagcaagtc cgcgacgtcc atcgctacgc cgatgccgtt gtaattggct
      901 cggctatcgt ctctgaaata gagcgactgc aaggagcgac cacagactta ccgaaaaaaa
      961 ttggagactt tacaaggagt ctcattccac cgtattcttg cgcggacttg acgtgaacgc
     1021 attgatggac gcccagtggg ccagaacgca gagagggctg cgacggcgcg cgccaagtcg
     1081 gcatttccga aacctcaccc tctggaaaat agtgctgttg caagtgtgaa acactgttcc
     1141 attctcgctc tccctgtggt atgctctccg ctcaccgggg gcgctttgac caaatacgac
     1201 tgcgagaaga ggagcggaag aaagagatgt atgccatggc tgatgaccgg atgacagcag
     1261 agcgcggcag ggcaatcgaa gctgcattag caaatattga aaagaagttt ggcaaaggat
     1321 cgatcatgcg cctgggcgag cgcgacgtgt ctgacatacc tgcaatctcg actacttcac
     1381 tcagtttgga cgccgctatc ggtgtcggcg gcgtgccgcg cgggcgcatc gtggagatct
     1441 atggaccgga gagttccggt aaaaccacgc tcgctctgca catcgttgct gaggcgcaga
     1501 aagccggtgg agtcgcggct tacatcgatg ccgaacacgc gatggatgct gactacgcgg
     1561 gaaaactcgg cgttgacatc gaccagttgc tgatttcgca acctgactcg ggcgagcagg
     1621 cgctcgagat tgccgaagcg ctggtacgtt caaatggtgt cgacgtgatc gtggtcgatt
     1681 cggtggcggc gctggtcccg cgcgccgaac tcgacggcga gatgggcgac tcgttgccgg
     1741 gtttgcaagc gcgtttgatg tcgcaggcgc tgcgtaaact cacggcgatt gtcgcgcagt
     1801 ccaatacttg tttcattttc atcaaccaga ttcgcgagaa gattggcgtg atgtttggca
     1861 atccggaaac aaccaccggc ggccgcgcgc taaagtttta ctcttctttg cgactggaca
     1921 ttcgtcgcat cggcgccatc aaggatggtg accgcgtggt tggcaaccgt acgaaagtga
     1981 aagtcgcaaa gaacaaagtg gcgccgccct tccgcgagtg cgagttcgac atcatgtacg
     2041 gcgagggtat ttcgcgtgaa ggcgacctgc tcgatctcgc cgtggctcaa cgcatcgtgg
     2101 aaaagagtgg cgcctggttt tctttcaagg gcgaacggct gggccagggc cgcgagaatg
     2161 ccaaacaggc attgagagag aatgcggaag tgttgaaacg aatcgaacga gaagtgaaag
     2221 ggaagttggg aattccggtt cgcgaggaac ccgctgcgcc atcgaacgta aaagcggcgt
     2281 cgaagtaaag tcgcgactaa acaattgagc gctcggcgag gatgttttat gctcgccgag
     2341 cgttctcttt gtggtctatg tcttcgattc tcatcaaaaa cggtacgctc gtcaccatgg
     2401 actccgccaa ccggatcgtc cgcggcgacc ttctcatttc cggttcgcac atcgtcgagc
     2461 tcggcgatat ttcgcaaagt gcggacgttg taattgacgc cggcggttgt gcccttatcc
     2521 ccgggtttgt tcagactcac attcatcttt gccagactct gtttcgcggc gccgcggacg
     2581 accttgcgtt gatcgactgg ttgaagaaac gcgtgtggcc catggaagcc gcgcactcag
     2641 ctgcgtcgat cgcggcctct gcgcgactcg gcgtcgcgga actgatcaaa ggcggcacga
     2701 cgtgcgcgtt aacgatggag actgtgaacc acaccgcgaa ggtgttcgag gttgttgaag
     2761 agactggttt tcgcgcgact gtcgggaagt gcatgatgga caaaggcgac gaagtgcccg
     2821 aggctttgca cgagcagacc gccgagtcga ttgaagagtc gatcgcgctg ctcgagcagt
     2881 ggcatggtaa agcgaacggc cgcattaggt actgtttcgc gccgcgtttc gcgatcagtt
     2941 gtactcgcga gttgctcgag aaggtcggcg aactcgcgcg tgtgcatagc gtgatgattc
     3001 acacgcatgc ctcagaaaac cggacggaat gtgcgttggt tgagcaagag acggggttgc
     3061 gtaacatcgt gtatctcgac agcgtgggca tttcgggacg tcacgtcgca ctggcccatt
     3121 gcgttcacct taacaacaat gagatcgagc ttctgaaaaa tacgcgcacg aacgtggtcc
     3181 actgcccgtc gtcaaacctc aagctcggtt cggggatcgc accgattgcg aaactgttgg
     3241 atgagggtat ttctgtatcg ctcggtgcgg atggtgcggc gtgcaacaac cgcctcgaca
     3301 tgtttaccga gatgcgcacg accgcgttgc tccagaaggc gttgcacggg ccggaggtgt
     3361 tggcggcaaa tcgtgtcctg cgaatggcta ctatcgacgg cgcaaaggcg cttgggttgg
     3421 aagctgagat tgggagtttg gaggttggca aacgagcgga cgtggcagta gtgaaattag
     3481 gacaagtgca taccgcgcct gaggctgacg tggtgtcgtc gctggtttat tccgcggtgg
     3541 cgagtgacgt gcagagtgtg gttattgacg gccggatggt gatgcgagat cgggagttat
     3601 tgacgattga tgaggcagaa acggtcgttg aggcggatgc tcaagcggag ttgctgatga
     3661 cgagggctgg tttaacgacg gattagcacg gatgtgagtt taaaaaagct gtgccagctc
     3721 gaaggtagcg actgcggggg gcgcgatcgg cggccgagct ggca
//
LOCUS       DASTEY010000678         5737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_527125, whole genome shotgun sequence.
ACCESSION   DASTEY010000678 DASTEY010000000
VERSION     DASTEY010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527125"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFO72_11800"
     CDS             <1..488
                     /locus_tag="VFO72_11800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9480025.1"
                     /translation="RADANAFALPGGYVYVTSGMLRRVRNDSELAGILAHEITHITLR
                     HGTNQASKAMLAQMPLQLLGGRFGGGLVGNLAQLGLSFGLNSLFLKYSRSAETQADVG
                     AVQLMRRAGYNPNGLVGFMQTLNNRGGGFFSDHPSPSNRIGRIQQEIAATGGRGSFRR
                     P"
     gene            complement(583..2082)
                     /locus_tag="VFO72_11805"
     CDS             complement(583..2082)
                     /locus_tag="VFO72_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9480026.1"
                     /translation="MKRNTMKKFLFVLLLIFAMSVPLAAQVVDCGCEDKPLPEILAVI
                     NGVKINRQDLSPQTRSRVEELQRQVIESRQRELDLQIDSMLLESEAKKRGVTPSQVIK
                     DEVIAKVQAPTEAEAQAFYDKNKSSIKAEFKDAKTEILEYLRYGRQQEQAQKLAERLR
                     AAAQVKVIAKPSAPPATSAERARVLAMVNDKQITMGDIENSLRPLIFNVQEQVYALRK
                     QDVDLKVNDTLLAQEAQKKGVTTRALLETEVSAKVPRITDAQAQAFYDENKERISGEF
                     AQTKAQIIQYLQERKEQEATLAFAEQLRRAAAIQINLTAPESPAFSIATDDQPVKGTA
                     NALVTIVAFTDFECPSCARQHPVLEKIVSEFGDRVRLVVRDFPLSQHANARKAAEAAE
                     AAREQDKYWEYVSVLFRNQSALGLDKLRQYATELGLDRARFDESLDSGKFAEKVQRDV
                     IDGRKLGVHGTPTLYINGKRLSDNSYESLKSVIESALKAEAAVRTSDEK"
     gene            complement(2093..2776)
                     /locus_tag="VFO72_11810"
     CDS             complement(2093..2776)
                     /locus_tag="VFO72_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9480027.1"
                     /translation="MTRQVFIMLTLLAAVFTTTHNALGQTPVAGQAPAEKPAPQTKPA
                     APPAPHDHSHVNKTEETTSQAHQYFSDVELINQDGKKVRFYSDVLKGKTVVVNAFFTS
                     CTSVCPPMNRNMQKIQEALGDRVGRDVFFVSITVDPEMDTPARLKEYAKSFHAGPGWV
                     FLTGKKENLDWALYKLGQYVERKDDHKTIFIVGNEPTGLWKKAFGMANVAELVEVVKS
                     VVNDKGATN"
     gene            complement(2776..3429)
                     /locus_tag="VFO72_11815"
     CDS             complement(2776..3429)
                     /locus_tag="VFO72_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9480028.1"
                     /translation="MKTQRALFYRLTLGFLVCTIALISAQAQDHPPHHKHNAPVEKVS
                     SGQVSKMNIPDVELLDQNGRKIRFYTDLVKDQTVAINFIFTTCTTICPPLGATFARVQ
                     KELGNKVGRDVRFMSISVDPATDTPERLKAWGAKFHAGDGWTFVTGNKPQLDELLRAL
                     GASSARREDHSPTVIIGNDAHGNWTRTYGLAKTTQLVQIINDVMAGKSSTTTAEVSN"
     gene            complement(3679..>5737)
                     /locus_tag="VFO72_11820"
     CDS             complement(3679..>5737)
                     /locus_tag="VFO72_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HET9480029.1"
                     /translation="NIKQNNGRTLFVFEESPELRTAILDFANDAVVAVRSFCSTMRDL
                     KAITRVLIFFVATVASASPALAQKKTAPQKTAPQKIERQGVEIEFTIEPIVEPGKASE
                     LMEAKEAVVRFKIQDKATKTPLSGVKPAVWLTQRGDAQPDQNACREKIQSFLQGSLRS
                     RPDVDLNAYYLLALNEEANISVIDPLLGFGTSKLLTLVMMKSPGEDWAQKTNDDKLFV
                     SMPLANQIAVVNTGTWKVVANIDTGMKPARVRLQPDQRYLWVGNDAGSADKPSGVTVI
                     DTATLKVVAQLATGNGHHELAFSSDNRFAFITNEEDGTLSIIDIQKLAKIKDLKTGAP
                     ATSVTFSALGNALYLVNEAEGTIAVVDARSHEIATRIQAKPGIRNLRFAPGGRWGFLP
                     NPKDNVVYVFDASTNRLAHTISVDKGPDQVAFTDTFAYVRSTGSTEVSMVRLSTLTAQ
                     PDVAKFPGGQTSPAEAKIEPSVADMMVPAPEGNAVLVANPADRVIYYYSEGMAAPMGS
                     FQNYRRNPRAVMVVDRSLREISSGVYSTTTRLPKSGIYDVAFLLDSPRITHCFNAEAK
                     PNPDVPREKQVAVRIEYLNKDKHPRVGEDYKLRFKLFDTATSKAKSDLKDVRVLTLLS
                     SGIWQKRDFARSVGDGVYELDLRVPHTGLYFIFVESRSQGVAFRQLPYLTLHAAATAA
                     VNK"
BASE COUNT         1137 a         1593 c         1592 g         1415 t
ORIGIN      
        1 atcgagcaga tgcaaacgcc ttcgcactgc cgggtggcta cgtttacgtc accagtggaa
       61 tgctgcgccg tgttcggaac gactccgaac tggcgggaat tctggcgcac gagatcacgc
      121 acataacgtt gcgacacgga accaatcaag cgtcgaaggc gatgcttgcg caaatgccgt
      181 tgcagctact gggcggcagg tttggtggcg ggcttgtggg aaatctcgcc cagctcgggc
      241 tctcgtttgg gttgaattcg ttgtttctca aatacagtcg tagcgccgaa actcaagcgg
      301 acgtcggagc ggtccaattg atgcgccgcg caggctataa ccctaacggc ctggtcggtt
      361 tcatgcagac gctgaataat agaggcggcg gattcttctc cgatcaccca agtccctcaa
      421 atagaatcgg ccgaatccag caggagatcg cagccaccgg cgggcggggc agtttccgcc
      481 ggccgtagtc gcagttttct ttcgcgatag agtcctcctc aagttaatgg gcagccgcgt
      541 gacgcgagct gcccattaag tcacgcccaa ctgagagcga gattattttt cgtcgctcgt
      601 tcttactgct gcctctgctt tcagagctga ctcaattaca gacttcagac tttcgtagga
      661 attgtcggac aggcgcttgc cattgatgta gagcgtaggg gtgccgtgga caccaagctt
      721 gcgtccatcg atgacgtcgc gctgaacctt ctcggcgaac ttgccactgt cgagcgactc
      781 gtcaaatcgg gcacggtcca gaccaagctc agtcgcgtat tgcctgagct tgtcgaggcc
      841 gagcgcagac tgattacgga aaagaacgct gacgtattcc cagtacttat cctgctcgcg
      901 agcggcctcg gccgcttcgg cagccttgcg cgcgttcgcg tgctgtgaaa gcggaaagtc
      961 ccgcacaacg agtcgcacgc gatcgccaaa ctcgctcaca atcttctcga gtaccggatg
     1021 ttgtcgtgcg caactcgggc attcgaagtc ggtaaacgca acgatggtga ctaacgcatt
     1081 cgcagttccc ttcaccggtt gatcgtctgt ggcgatcgag aaagcgggcg actcaggagc
     1141 agttagattt atctggatcg ccgccgcgcg tcgcaattgc tcggcgaaag cgagagtcgc
     1201 ttcctgctcc tttcgctcct gcaagtactg aatgatttgc gccttggtct gggcgaactc
     1261 gccgctgatc cgctctttgt tttcgtcata aaacgcctgt gcctgcgcgt ccgtaatccg
     1321 cggcactttc gcgctgactt ccgtctcgag cagcgctcgc gtggtgacgc ctttcttttg
     1381 tgcttcctgc gcgagcagcg tgtcgttcac tttcaagtct acgtcctgct tacgcagcgc
     1441 gtagacctgt tcctgaacat tgaaaatcaa aggacgcaaa ctgttttcga tgtcacccat
     1501 cgtgatctgc ttgtcgttga ccattgccaa cacgcgtgcg cgctctgcgc tggtcgcagg
     1561 tggcgccgat ggcttggcga taaccttcac ctgggccgca gcccgcagac gctcggcaag
     1621 tttttgcgcc tgttcctgct gccgtccgta acggagatat tccaggatct cggttttagc
     1681 atccttgaat tccgctttga tacttgactt gttcttatcg taaaaggcct gcgcctcggc
     1741 ttcggtcggc gcctggacct tggcgatgac ctcgtctttg atgacttgcg acggagtaac
     1801 tccgcgtttc ttcgcttcgc tttccagcag catcgaatcg atctgtagat cgagttcgcg
     1861 ctgacgggac tcgatcacct gccgctgcaa ctcctcgacg cgtgatcgcg tttgcgggct
     1921 gagatcctgc ctgttgatct tcactccatt tatgacggcg agtatttccg gaagtggttt
     1981 atcctcgcag ccgcagtcga caacctgagc agccaggggc acgctcatcg caaatataag
     2041 tagaaggacg aataaaaatt tcttcatagt gttccgtttc atctcttagg gattagttcg
     2101 tagcgccttt gtcgttgacg acgcttttga cgacttccac aagttcggcc acgttcgcca
     2161 ttccgaacgc tttcttccac agtcccgtcg gttcgttgcc gacaatgaaa attgttttgt
     2221 ggtcgtcttt tctctcgacg tattggccca gcttgtacag cgcccaatcg aggttctctt
     2281 tttttccggt cagaaaaacc cagcccgggc cggcatgaaa actcttcgca tactctttca
     2341 gccgtgcggg cgtatccatt tccggatcga ccgtgatgga aacgaagaat acgtcgcggc
     2401 cgacgcggtc gcccaaagct tcctgaatct tctgcatgtt gcggttcatc ggcgggcata
     2461 ccgaagtgca actcgtgaag aaggcgttga cgacgaccgt cttgcctttc agcacgtccg
     2521 aataaaaacg cactttcttg ccgtcctggt tgatcagctc gacgtcactg aagtactggt
     2581 gcgcctgcga cgtcgtttcc tcagttttat tcacgtgcga atgatcgtgc ggcgccggtg
     2641 gagccgcagg tttagtttgc ggcgcaggct tttccgctgg tgcctgcccc gcgaccggcg
     2701 tttggcccaa agcgttgtgt gtcgtcgtaa aaacggcagc cagcaatgtg agcattatga
     2761 aaacttgtcg tgtcattagt tcgatacctc agcggttgta gtactactct ttcctgccat
     2821 gacgtcgttg atgatttgga ctaactgggt cgtcttcgcc aaaccgtaag tacgcgtcca
     2881 gttgccatgc gcgtcattgc cgatgattac agtaggcgaa tgatcttcgc ggcgcgcgga
     2941 agacgcgccc aacgcgcgca gtagttcgtc aagttgtggt ttgtttccag tgacaaaggt
     3001 ccaaccgtca cccgcgtgga acttcgcgcc ccacgctttg agccgttcgg gagtgtcggt
     3061 cgccggatcg acgctgatgg acatgaagcg aacgtctcga ccgactttat ttccgagttc
     3121 tttttgcacg cgtgcgaaag ttgcgccgag cggcgggcag atcgtcgtgc aggtagtgaa
     3181 gataaaattg atggcgactg tctggtcttt gacgagatcc gtgtagaaac gaatcttccg
     3241 tccgttctgg tcgagcagtt cgacatccgg gatattcatc ttcgacactt gcccggacga
     3301 taccttttcc accggtgcgt tatgtttgtg atgcggcgga tgatcctgcg cctgcgcgga
     3361 aatcaaggca atcgtgcaaa caagaaagcc aagagtcagg cgataaaaaa gtgcgcgttg
     3421 tgtcttcaaa tacatatctt ctccagtatc atcaggcttt gcctcaaaag tcttctttca
     3481 ttctcaccgg gcttcagccc ggtgttgccg ccacgcggtt ttgagaaacc gttttaccgg
     3541 tttcgctggc gaatcaacgg aaaaccgttg aaacggtttc ctccgtcttc actgctcctg
     3601 caccggctaa agctcggtga gaatgagaga ggacttttga ggcaaagcag tatcatcagg
     3661 tgaattctgg taatcctgtc acttgttgac tgcggctgtt gccgctgcgt gtaacgtcag
     3721 gtaaggcagt tgccggaaag ccactccctg cgatcggctt tcgacgaaga tgaaatacaa
     3781 gcccgtgtgc ggaactctta agtcgagttc atacacgccg tcaccgaccg aacgcgcgaa
     3841 gtcgcgtttc tgccaaattc ctgacgacag caacgtcaag acacgtacgt ccttcagatc
     3901 gctcttcgct ttcgacgttg cggtgtcgaa gagtttgaag cgcagtttgt agtcctcccc
     3961 gacgcgcggg tgcttgtcct tatttagata ctcgatgcga acggcgacct gtttttcgcg
     4021 cgggacatcc ggattcggct tcgcctcagc attgaagcag tgcgtgattc ggggcgagtc
     4081 gagcaggaaa gcaacgtcat agatgccgct cttcggcaat ctcgtcgtgg tggaatagac
     4141 gcccgaactg atttcacgca acgagcgatc gaccaccatc accgcccgag gattgcggcg
     4201 gtagttttga aaactcccca tcggagccgc catgccttcc gagtagtaat agatgacgcg
     4261 gtcagccgga ttcgcgacaa gcacagcgtt gccttcgggc gcaggcacca tcatgtccgc
     4321 gacgcttggt tcaatcttcg cttccgcagg ggaggtctgc ccgccgggaa atttcgctac
     4381 atcgggttgg gctgtcagcg tgctgagacg gaccatgctg acctcggttg atccagttga
     4441 gcggacatag gcgaacgtgt cagtgaacgc gacctggtcc gggcctttgt cgacgctgat
     4501 cgtgtgcgcc agacgattcg tcgaagcgtc gaagacgtat acgacattgt ctttcgggtt
     4561 tgggagaaaa ccccagcgac cacccggcgc aaagcgcaaa tttctgattc ccggcttcgc
     4621 ttgaatacgc gttgcgattt catggctgcg cgcgtcgaca acagcgatag ttccctcggc
     4681 ttcattcacg agatagagcg cgttgccgag cgccgagaag gttaccgatg tggccggagc
     4741 acccgtcttg agatctttga tcttcgcgag tttctgaatg tcgatgatcg agagcgtgcc
     4801 gtcttcttcg ttggtgatga acgcgaaccg attgtcactg ctgaaggcga gctcgtgatg
     4861 tccgtttcca gttgccaact gggccaccac tttcagtgtg gccgtatcga tcaccgttac
     4921 gccgctcggc ttatcggcgg aacccgcatc gttgccgacc cacaggtaac gctgatcggg
     4981 ttgcaggcga acacgcgcgg gcttcatacc ggtgtcgatg ttggcgacta ctttccacgt
     5041 accggtgtta acgacagcaa tctggttggc gagtggcatg ctgacgaaaa gcttgtcgtc
     5101 gttggtcttc tgcgcccagt cttcaccggg actcttcatc atcacgagcg tcagtagctt
     5161 gctcgtacca aaaccgagta aaggatcgat gactgagata ttggcttctt cattaagcgc
     5221 gagcaggtag taggcattca gatccacatc ggggcgtgaa cgaagactgc cctggagaaa
     5281 cgattggatc ttttcgcgac atgcgttctg atccggctga gcgtcgccgc gttgtgtcaa
     5341 ccacacggcg ggtttcacgc cggagagcgg agtcttggtc gctttgtcct gaatcttgaa
     5401 acgaacaacc gcttctttcg cctccatcaa ttccgacgct ttgcctggct ctacgatggg
     5461 ctcgatggtg aactcgatct cgacgccctg tcgctcaatt ttttgtggcg ccgtcttttg
     5521 tggcgcggtt tttttctggg ccaatgcggg acttgcgctg gcgactgtcg ccacgaaaaa
     5581 tatcaacacg cgcgtaatcg ccttcaagtc tcgcatcgtg ctgcaaaagg agcgcacagc
     5641 cacgaccgca tcattggcga agtcgagaat ggcagtgcgc agttcaggcg actcctcgaa
     5701 aacgaagagt gtgcgtccgt tattctgctt gatgttc
//
LOCUS       DASTEY010000679         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1416177, whole genome shotgun sequence.
ACCESSION   DASTEY010000679 DASTEY010000000
VERSION     DASTEY010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416177"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..335
                     /locus_tag="VFO72_11825"
     CDS             165..335
                     /locus_tag="VFO72_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480030.1"
                     /translation="MTIILIPLTFSITQGILWGFIAHVGLYLATGRRKDVHPVMYVLA
                     VVAIALLWLEHT"
     gene            332..>3703
                     /locus_tag="VFO72_11830"
     CDS             332..>3703
                     /locus_tag="VFO72_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480031.1"
                     /translation="MKNVRVYPWLLIVLLAGNSSAQSPSPTPPDTRGLGIQRTDPTST
                     TQTTQQSREAKPELVLQTGYNNIFGATRLVFSPDGRLLATGTFRSNTIMLWETATNRK
                     LRDLSGSGQTAPGLAPAIAFSRDSRLIAASAADNSVRIWDVNSGRELHTLAGPQGTLT
                     AAAGVYFIGFASDNRLVTVSDTARVWDLNSGRELRTLPLSSPEMTGYTGNDGGMAISN
                     DGTQFVMLTDDAEAHARVLDLASGREVRRVKLSDKQIDSINFAFSPDGRVLAAGIVEK
                     RLKLWDLTTKKEQNLGQTTKEFSQLKFSRDGRLLALSENYLVKIWDAATGRELTQLKV
                     PNSGAFVTQADAFIAFSDDGKRLATGGFDTDTIIWEIETGKRLSNLSGRTNMAYNVAF
                     SADGTQLSSGGRTRWDLRTGRGLRVVPASTSSEKTYGIPSPDGRLVVVMKPNSSVLSL
                     VETPSGRQLQTLTPSGETGVVQRMRFSADGTMLAVVYGTVETHGGQVTVRPGSLMRGS
                     QVKIWDVKTGRELHALTLNDIPAEADFSADGRVLATIGTMGQTSLWDVQSGSKLRDLT
                     ISPIVGLTTMPTAPIKPGQMPTMPNMADIAAMMTNIMGTMAAGTMGRTVTSVAFSPDG
                     KTLVTGGVESKTNIDLAAMMSGQRPKNQKGSKQPDPADFMKDLKVEAVGQVQIWDIAT
                     GREIGKLTGHGRAVGKVVFSRDGKLLATGGTDNTIKVWDLATKRELATFTGHTANIES
                     LDFHPDGRLLASASEDGSTFLWDTKTGEHLLTLISLDDGGEWMVVTPQGLFDGTPVSW
                     NQILWRYNQETFNVAPIEWFFNEFYYPGLLSDVFAGKRPRVAQDVSKKDRRQPVVKLS
                     LSGQQVAPQASDAAIASRTTKVKIDITDAPADKDNSQGAGARDVRLFRNGSLVKVWRG
                     DVLKGQSSVSLEEEITVTAGPNRLVAYAFNRDNMKSKDAPLVFTGAESLKRKGTAYII
                     AVGVNEYENPQYNLKYATADARSFGDEMRGRQQQLGEFDRVEVVQLLDKEATKANILT
                     AIKRLAGEPGPPSLKAGPLDALKRVEPEDTVIIYYAGHGTAQAQRFYLIPHDLGYTGD
                     RTKLTEAGLKTMLAHSISDLELEAAVEGLDAGHLLL"
BASE COUNT          819 a          997 c         1120 g          767 t
ORIGIN      
        1 tgtttaccgc gctctgtttt ttgccgtgct tttttctggc gccgctggcg gcgatggttc
       61 caccgttcgc caccgcgccg gttttgattt tggttggcgc gtcgatgttc aaaagcgtgg
      121 gtgaaataag gttggcgaga attgaagagg gattgcctgc gtttttgact ataatcctca
      181 tcccccttac gttttccatc acccagggaa tactctgggg cttcatcgcg cacgtcggcc
      241 tttatctcgc gaccggcaga cgaaaagacg tgcatccggt aatgtatgtg ctggccgtcg
      301 tggccattgc gttactttgg ttggaacaca catgaaaaac gtccgtgtct atccgtggct
      361 gttaatagtt ctcttggcag gtaactcatc agcccaatca ccatctccca cgccccccga
      421 tacgcgcggc ttaggaatac agcgcactga tccaacttcc accacacaaa ccacccagca
      481 gtcacgcgaa gcaaaacccg aactcgttct ccagaccggt tacaacaata tcttcggcgc
      541 cactcgcctc gtcttcagcc ccgatggacg acttctcgcc accggcacgt ttcgcagcaa
      601 cacgatcatg ctctgggaga ccgccacgaa tcggaaactg cgcgatctct caggcagtgg
      661 ccagactgcg ccgggtcttg cgccggcgat tgcgtttagt cgcgacagcc gtctgatcgc
      721 cgcttcggct gcggataact cggtcaggat ttgggacgtc aacagcggtc gcgaactgca
      781 tacgctcgct ggtccacaag gaacgctgac agcagcagcc ggcgtctact tcatcggctt
      841 tgcttcggat aacagactcg taactgtcag cgacacggcg cgagtttggg atctcaacag
      901 cggacgcgaa ttgcgcacgc tcccattgag ctcgcccgag atgaccggtt acaccggcaa
      961 cgacggggga atggcgatct ctaacgacgg cacgcagttc gtcatgctta cagacgatgc
     1021 cgaggctcat gctcgcgttc tcgatctcgc gagcgggcgc gaagtgcgtc gtgtgaagtt
     1081 gtctgacaaa cagatcgatt cgatcaattt cgctttcagc ccggatgggc gcgtgctcgc
     1141 tgccgggatt gtcgagaaac gtttgaagct ttgggacctg acgacgaaga aggaacagaa
     1201 cctgggccag accacgaagg aattcagcca gctcaaattc agccgggacg gccggctgct
     1261 ggcgttgtcg gaaaattacc tggtgaagat ttgggacgct gcgactggtc gcgagttgac
     1321 gcagttgaaa gtgccgaaca gcggtgcgtt tgtgacgcag gccgacgcgt tcatcgcgtt
     1381 cagcgatgac ggaaaacgac tcgcgacggg tggtttcgac actgacacga tcatttggga
     1441 gatcgaaacc ggcaagcgtc tctcgaattt gagtggccgc actaacatgg cctacaacgt
     1501 cgcgttcagt gcggatggga ctcagctttc gtcgggtggc cgcacacgtt gggacctgcg
     1561 caccggtcgc ggcttgcgag ttgtgccggc atcgacgtcg tcagagaaga cttatggaat
     1621 tccgagtcct gacggaagac tcgtcgtggt gatgaagcca aacagcagcg tgctctcgtt
     1681 ggttgaaacg cccagcgggc gacagttgca aacgctcacg ccgtcaggcg aaacgggcgt
     1741 cgtgcagcga atgcgcttca gcgcagatgg aacgatgctg gcggtcgttt atggaacagt
     1801 tgaaacgcac ggagggcaag taacagtccg gccaggatca ctcatgcgcg gcagtcaggt
     1861 gaagatttgg gacgtcaaga caggtcgaga attgcacgcg ctaactttga acgacatacc
     1921 ggcggaagca gatttcagtg ctgatggtcg tgtgcttgca acgatcggaa cgatgggcca
     1981 gacctcactt tgggatgtgc agtcgggaag caagctgcgc gacctgacga tctctccaat
     2041 cgttgggttg acgacaatgc cgacggcgcc gatcaaaccc gggcagatgc ctacgatgcc
     2101 gaacatggcc gatatcgccg cgatgatgac aaacatcatg ggcacaatgg cagccggaac
     2161 catgggccgc actgtgacct ccgtcgcctt tagtccagac ggcaaaactt tggtgacggg
     2221 tggcgttgag tctaaaacca acatcgatct ggcggcgatg atgagtggcc aacggccaaa
     2281 gaatcagaag ggctcgaagc aacctgatcc ggccgacttc atgaaggact tgaaagtaga
     2341 agctgtgggc caggtgcaga tttgggacat agcgaccgga cgcgagatcg gaaagttgac
     2401 aggtcacggg cgcgcggtcg gcaaggtggt gtttagtcgt gacggcaaac tactcgctac
     2461 cggcggcacg gacaacacga tcaaagtctg ggaccttgcc acaaaacgtg aactcgccac
     2521 cttcacgggg cacacggcaa atatcgaatc gctcgacttt catcccgatg gccggctgct
     2581 cgcatccgca agcgaagatg gcagcacgtt tttgtgggac acaaaaaccg gcgagcactt
     2641 gttgacgctg atttcgctcg acgatggcgg cgaatggatg gtggttacgc cacagggttt
     2701 gtttgacggc actcccgttt cgtggaacca aattctctgg cgctacaacc aggagacgtt
     2761 caacgttgcg ccgatcgagt ggttcttcaa tgagttttac tatccgggtt tgttgtcgga
     2821 cgtttttgcc ggcaagcggc cgcgtgtggc ccaggacgtc tcgaagaagg atcgacgaca
     2881 gccggttgtg aagttgtcgc tgtcagggca gcaagtcgcg ccgcaggcgt ctgatgctgc
     2941 gattgcctcc cgcacaacaa aagtgaagat cgacatcact gacgcgccgg ccgataaaga
     3001 taattctcaa ggtgcgggcg cgcgcgatgt acgtctcttc cgtaacggat cgctcgtgaa
     3061 ggtctggcgc ggagatgtgc tgaaaggtca gtcatcggtt tcactcgaag aggagatcac
     3121 cgtgaccgcc ggaccaaatc ggctggtggc ttatgcgttc aatcgcgaca acatgaagag
     3181 caaagatgcg ccgctggtgt ttacgggcgc tgagagtttg aagcgcaaag gcaccgcgta
     3241 cattattgcc gtgggcgtca atgagtatga gaatccgcag tacaacttga agtacgccac
     3301 cgcggatgca cgcagttttg gcgacgagat gcgtggccgg cagcaacagc tcggcgagtt
     3361 tgatcgtgtt gaggtagttc aactgctcga taaggaggcg acgaaggcaa acattcttac
     3421 cgccatcaag cggcttgcag gtgagcctgg gccaccgtcg ttgaaggccg gaccattaga
     3481 cgcgttgaag cgtgtcgagc cggaagacac cgttatcatt tactacgctg ggcacggcac
     3541 tgcgcaggcg cagcggtttt atcttattcc ccacgacctc ggctacacgg gcgatcgcac
     3601 gaagttgacg gaagcaggac tgaagacgat gctggcgcac agtatttcgg acctcgagtt
     3661 ggaagctgcg gtcgagggac ttgatgcggg tcacctgttg ctg
//
LOCUS       DASTEY010000680         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_213373, whole genome shotgun sequence.
ACCESSION   DASTEY010000680 DASTEY010000000
VERSION     DASTEY010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213373"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFO72_11835"
     CDS             complement(<1..935)
                     /locus_tag="VFO72_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9480032.1"
                     /translation="MLALRVNKKRLSIKNIQKPDRADEALVRVLLSGICNTDLEIARG
                     YAGFKGTIGHEFVGVVEESTDRSLAGRRVVGEINAGCGKCDLCRAGDSRHCASRTVLG
                     IVGRDGAHAEFLQLPVENLLLVPDKIPDEHAVFTEPLAAACGILERVKIDPSDRVAVI
                     GDGKLGLLCAQVVALSGADMLLVGKHSSKLRIAERRGIETATPRQAAKRKRQFDVVVE
                     ASGAATGFGLALDLLRPRGQLVLKSTFHGKTELDAARIVVDEVSIVGSRCGRFTPALD
                     LLNKAAVDVDSLISEEYPLSNGVHAMRRAGTRGVL"
     gene            complement(970..1485)
                     /locus_tag="VFO72_11840"
     CDS             complement(970..1485)
                     /locus_tag="VFO72_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480033.1"
                     /translation="MLSQSSVSPKFRGLLIRLLQVGLILVLVGAGWLIYRQLPDGGAD
                     VSSNQGTSTLQIFIRQTPETVGPALDVAVSLYPVDIVAVRHEFFTEQRPGKRFEDFLK
                     ERMKGRSPINARLDKQGQGAVTLAPGSWWLHATLSGDEQLEWRLPVTVTGSKQVIELT
                     PKNAYTRSRSF"
     gene            complement(1501..1845)
                     /locus_tag="VFO72_11845"
     CDS             complement(1501..1845)
                     /locus_tag="VFO72_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9480034.1"
                     /translation="MQIYALVLLTLAGLSSFAIAEPAAPSNARTAGQLFRRYCVSCHG
                     NDGKAKTSKGRFSHARDLTDPQWHADVSDERIFNSIMNGRDTGGNMPAFGNKMKEKEI
                     DSLVTFVRGLKG"
     gene            1959..>2209
                     /locus_tag="VFO72_11850"
     CDS             1959..>2209
                     /locus_tag="VFO72_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480035.1"
                     /translation="MTEIEITKLREECGVFGIYGHPEAARLTYLGLYALQHRGQESCG
                     IVASDGAELRMERAMGHVSEAFDQTLLDRLRGSSAIGHVR"
BASE COUNT          477 a          642 c          630 g          460 t
ORIGIN      
        1 ttcagcactc cgcgagtgcc ggcacggcgc atcgcatgga cgccgttcga gagcggatac
       61 tcttcgctga tcaggctgtc gacatcaacc gcggctttat tcaacagatc gagggcgggg
      121 gtgaagcgcc cacagcgcga accgacgatt gaaacttcat caaccacgat ccgtgcagcg
      181 tcgagttctg tttttccgtg gaacgtcgac ttcagaacca gttgacctct gggacggagg
      241 agatcgagag ccaggccgaa tccggttgcg gcccccgacg cttcgaccac cacatcaaat
      301 tgccgcttgc gtttggcagc ctgtcgagga gttgcggttt cgatgccgcg tctttcggcg
      361 atgcgcaact tggaggaatg ctttccaacg aggagcatat cagccccgga gagcgcgacg
      421 acttgcgcgc aaagtaaacc gagtttgccg tcgccaatga cagcaacacg atcggaaggg
      481 tcgatcttta cgcgctcgag gatgccgcac gcggcggcga gaggttcggt gaagactgcg
      541 tgctcgtcgg gaattttatc gggaacgagc aagagatttt caacgggaag ttgcagaaac
      601 tctgcatgcg caccatcacg cccgacaatg ccgagcacgg tgcgtgaagc gcaatgtcgc
      661 gaatcgcccg cgcggcacag atcgcactta ccgcaacctg cattgatctc accgaccacg
      721 cgacgaccgg caagcgagcg gtcagtactc tcttcgacga caccaacaaa ctcgtgcccg
      781 atcgttcctt taaaacctgc atagccacgc gcgatctcga gatcggtgtt gcaaatgcct
      841 gacaacaaca cccgcacgag cgcttcatca gcgcgatccg gcttctggat gtttttaatt
      901 gaaagacgtt tcttattgac gcggagggcg agcatttcag atccgtgttt tatccgcgtt
      961 catcggtggc taaaaagacc tgcttctcgt gtacgcgttc tttggcgtga gctcgatcac
     1021 ctgtttcgat cccgtgacgg tcaccggtaa acgccactcg agctgttcgt cgccggagag
     1081 cgtggcgtgc agccaccaac tgcccggcgc aagtgtcacc gctccctgtc cctgcttgtc
     1141 gagtcgcgcg ttgatgggcg agcgaccctt catgcgctcc ttgagaaagt cttcaaaacg
     1201 ctttccgggc cgttgctcgg tgaagaactc gtgtcgcaca gcgacgatat ccacgggata
     1261 caaactcacc gccacgtcga gcgctggacc gactgtttcc ggagtttgcc gaatgaagat
     1321 ctgtaaagta ctggttccct ggtttgaact gacgtcagcg ccgccatcgg gaagctgccg
     1381 ataaatcagc catcctgccc cgaccagcac gagaatcagc cccacttgca ggagccggat
     1441 cagcaggccg cgaaacttag gtgaaacaga actctgagac aacattgact gcgaccgcgg
     1501 ctatcctttc agcccgcgca caaaagttac cagcgaatcg atctcttttt ccttcatctt
     1561 gtttccgaac gccggcatat tgccaccggt gtcgcggcca ttcatgatcg aattgaagat
     1621 gcgttcgtcg ctgacgtcgg cgtgccactg tggatcggta agatcgcgcg cgtggctaaa
     1681 cctgcccttc gaagttttcg ccttgccatc gttaccgtga caggacacgc agtagcggcg
     1741 aaacaactgc cccgcggtgc gcgcgttact cggcgcggcg ggctcagcga tcgcgaacga
     1801 tgacaagccg gcgagagtca gcaaaactaa ggcatagatt tgcatacgcg agccatcata
     1861 acaaagatta gccgcagaag aacgcggacg cgcgctgatc agaatcggcg tgcatccgcg
     1921 ttcatctgcg gctcatactt tgttatgatt gcgaatcaat gactgaaatc gagattacta
     1981 agctacggga agagtgtggt gtcttcggaa tctacggaca tcccgaagcg gcgcgattga
     2041 cgtacctggg tttatacgcg ctccagcatc gtggtcagga atcgtgtggc atcgtcgctt
     2101 cggatggcgc cgagttgcgg atggagcgcg ctatggggca tgtctcggag gcattcgatc
     2161 agaccctcct cgaccgtttg cgcgggtcca gcgcgattgg ccacgtgcg
//
LOCUS       DASTEY010000681         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_954283, whole genome shotgun sequence.
ACCESSION   DASTEY010000681 DASTEY010000000
VERSION     DASTEY010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954283"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="panB"
                     /locus_tag="VFO72_11855"
     CDS             <1..723
                     /gene="panB"
                     /locus_tag="VFO72_11855"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9480036.1"
                     /translation="VVLGYGNTVPVTLDEILIHLKAVRRAVQRALLVADMPYGTFHTG
                     EDDAVKNALRLVKEGGAEAIKLEGGHKRVPLVKRLVDEEISVMGHIGLTPQSINQLGA
                     YRVQGKTPKAAQQLIDDAKALEDAGAFAVVLEVVPREIARIITETISIPTIGIGAGSH
                     CDIQVLVLHDMLGLSFGKQARFVRPYANLREVITDAVTRFADDVRNGTYPSQAESYAL
                     PAETAEALEIENPTRDSKAEKS"
     gene            738..1616
                     /gene="panC"
                     /locus_tag="VFO72_11860"
     CDS             738..1616
                     /gene="panC"
                     /locus_tag="VFO72_11860"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9480037.1"
                     /translation="MEIINRRQRMSSVARKIRREQDRTVGLVPTMGALHEGHLSLVRE
                     ARRMCDVVVVSVFVNPAQFGPGEDFEHYPRDLTKDTALLTDYNVDYIFAPTVEEIYPK
                     GFSTYVTVEGLSEQLEGASRPGHFRGVATVVTILLNTVRPDFAFFGQKDAQQALVIKR
                     LVKDLAFDTEIVVLPIVREDSGLAISSRNLYLNADEQASAGVIHRALVQAKAAYKDGE
                     RSAGRLADVIRSAIESEPRARLDYVAIADAETLDPVERIEERPTLIAVAAYIGKTRLI
                     DNTILNKVKKKEVAPQ"
     gene            1623..2141
                     /locus_tag="VFO72_11865"
     CDS             1623..2141
                     /locus_tag="VFO72_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9480038.1"
                     /translation="MNSKKQIRFRLNGEDTEVAFAPHKTLLEVLREDLNLTGTKHGCE
                     LGECGTCAVLVDGRSILSCLMLGLDAEGREVKTIEGMANGAELHPLQQTFADLGAAQC
                     GYCSPGFLLVAEELLKKSPDPSRTDIQEALSGNLCRCTGYIKIYEAVELAAARMRGEA
                     YELPKESIYGLD"
     gene            2159..>3289
                     /locus_tag="VFO72_11870"
     CDS             2159..>3289
                     /locus_tag="VFO72_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET9480039.1"
                     /translation="MDKSLNIVGKPFRKVDARAKCVGQTKFADDIVLPRMLFCRILRS
                     HVPHALIKSIDASKALAQPGVFAVITGRDLPIPYGILPVSQDEHALCIDKVRFVGDPV
                     AAVAAIDEDTAFDAMNLIEVEYEPLNTISNIEEAVLIDEPRIHEYGDSGNVHKKVSLE
                     FGNVDDGFADADLVREDVFFYEGNTHLPMEQHAAVAHFDADQKLTLWSSTQTPHYVHR
                     ALAKVLKMPASHIRVIATPNGGGFGGKSDPFNHEVAVCKLAMITGRPVKVTLTREEVF
                     YCHRGRHPVLMKVKTGVKNDGAITAMHFQSFLDGGAYGSYGVASTFYTGALQTVTYDV
                     PRYKFQGLRAFTNKPPCGPKRGHGTPQSRFGQEVQIDKIAERL"
BASE COUNT          742 a          863 c          988 g          696 t
ORIGIN      
        1 gtggtcctgg gttacggcaa caccgtgccg gtgacgctcg atgagatttt gatccatttg
       61 aaagctgtgc ggcgtgccgt gcaacgtgca ttgctcgttg ccgacatgcc ctatggaact
      121 ttccacacgg gcgaagacga cgcagtcaag aatgcgttgc ggctcgtgaa agagggcggc
      181 gcggaagcaa tcaaactgga aggcgggcat aagcgcgtgc cgctggtcaa gcggttagtt
      241 gatgaagaga tcagcgtgat gggccatatt ggtctgacgc cgcaatcgat caatcaactc
      301 ggcgcatatc gcgtgcaagg taaaaccccg aaagccgcgc agcaattgat cgacgacgca
      361 aaggctcttg aagatgccgg cgcttttgct gttgtgctcg aagtggtacc gcgcgagatt
      421 gcgagaataa ttactgaaac catcagtatt ccgacgatcg gtatcggcgc cggttcacat
      481 tgcgacattc aggtgttggt gctgcacgat atgcttggtc tatccttcgg caaacaggcc
      541 cggttcgttc ggccttatgc caatctgcgt gaggtgatca ccgatgccgt aaccaggttt
      601 gcggacgacg tgagaaacgg cacgtacccg tcgcaagcgg aatcgtatgc tttgcctgcc
      661 gagactgccg aagcactcga gatagaaaac cccacgagag actcaaaggc cgagaagtct
      721 taatcctcct caaaactatg gagatcatca accgccgtca gcgcatgagt tccgtcgcgc
      781 gcaaaatccg ccgtgaacag gatcgcactg ttggtctggt ccctacgatg ggagcactgc
      841 acgaaggcca tttgagtttg gtgcgtgaag cgagacgcat gtgcgacgtg gtcgttgtct
      901 cagtcttcgt caacccggcc cagtttggtc caggcgaaga cttcgagcat tacccgcgcg
      961 acttaacgaa agacacggcg ctgctgaccg attacaacgt cgactacatc ttcgcgccga
     1021 cagtcgaaga gatttatccc aaaggctttt cgacttacgt caccgtcgaa ggactttccg
     1081 agcagctcga aggagcttca cgaccgggac actttcgcgg cgttgcgact gtagtgacaa
     1141 tactgctgaa cacggtgcgg cctgattttg cgttctttgg tcagaaagac gcgcagcagg
     1201 cgctcgtgat aaaacggctc gttaaagatc tggcgttcga cacggagatc gtcgtgttgc
     1261 ctatcgtgcg agaggattcc gggctcgcga tttcgtcgcg caatctttat ttgaacgccg
     1321 acgaacaggc ttccgccggc gtgattcatc gcgcgctggt ccaggcaaaa gccgcgtaca
     1381 aagacggcga gcgcagtgcc ggacgactcg cggatgtaat tcgttccgca atcgaatctg
     1441 aaccgcgcgc gcgtttggac tacgtggcaa tcgccgatgc cgaaacactt gaccccgtcg
     1501 agagaatcga ggaacgaccg acgttgatcg ccgttgccgc ttatatcggc aagactcgtt
     1561 tgatcgacaa cacgattttg aataaggtta agaagaagga agtcgcaccg cagtaattcc
     1621 tgatgaacag taagaaacag attcgcttcc gtcttaacgg cgaagatacc gaagtcgcct
     1681 ttgcgccgca taagacgctg ctggaagtgc tgcgtgaaga tctgaatctt accggcacga
     1741 agcatggatg tgagctgggt gagtgcggca cgtgcgcggt gttggttgac ggacggtcga
     1801 tactttcgtg cctcatgctc gggctcgacg cggaagggcg tgaagtaaag actatcgagg
     1861 gaatggccaa cggcgcggag ttgcatccat tgcagcagac gttcgcggac ctcggcgcgg
     1921 cgcagtgtgg ttattgctcg cccggttttc tgctggtggc ggaagagttg ttgaagaagt
     1981 cgccggatcc gtcgcgcacg gacattcagg aagcgctctc ggggaatctg tgccggtgca
     2041 ccggttacat caaaatttac gaggcagtgg aacttgccgc cgcacgcatg cgtggcgagg
     2101 cctacgaact gccgaaggag agcatctacg gacttgatta agagagtctg tgtaatccgt
     2161 ggataagagt ctaaatattg ttggtaagcc gtttcgcaag gtcgacgcac gtgcaaagtg
     2221 tgtcggacag acgaagtttg cggacgacat cgtgctgccg cgaatgttgt tctgccgaat
     2281 actgcgctcg catgtgccac atgcgttgat taagagcatc gatgcttcca aagcgctggc
     2341 gcaaccgggc gtttttgctg tcataaccgg aagagatttg ccgattccct acggcatcct
     2401 gcccgtgagt caggacgagc atgcgctgtg tatcgacaag gtgcgcttcg tgggcgatcc
     2461 ggtggctgcg gtcgctgcga tcgatgaaga caccgcgttc gacgcgatga atttgatcga
     2521 ggtcgaatac gaaccgctca acacgatctc gaacatcgaa gaagcagtgc ttatcgacga
     2581 gccgcgtatt cacgaatacg gcgacagcgg taatgttcac aagaaagtat cgctggagtt
     2641 tggcaacgtc gacgacggat tcgcggatgc ggatttggtg cgtgaagacg tcttctttta
     2701 cgaaggcaac acgcacttgc caatggaaca gcatgcggcg gtggcccact tcgatgccga
     2761 tcaaaaactg acgctctgga gttcgacgca gactccgcac tacgttcatc gcgcgctcgc
     2821 caaagtcttg aagatgccgg cgagtcacat tcgcgttatt gccacaccaa acggcggcgg
     2881 cttcggcggc aagagcgatc ctttcaatca cgaggtcgcg gtttgcaagc tcgcgatgat
     2941 caccgggcgt ccggttaagg tcacacttac gcgcgaagag gtcttttatt gtcatcgtgg
     3001 ccgccatccc gtgttgatga aagtcaagac gggcgtgaag aacgatggtg cgatcaccgc
     3061 gatgcacttc caatcgtttc tcgacggcgg ggcgtatggg agttacggcg ttgcgagcac
     3121 gttctacacc ggcgcgttgc aaacggtgac gtatgacgtg ccccgctaca agtttcaggg
     3181 attgcgtgcg ttcaccaaca agccgccgtg cggtccgaaa cgcggccacg gcacgccgca
     3241 gtcgcggttc ggccaggaag tgcagatcga caagatcgcc gaacgcctc
//
LOCUS       DASTEY010000682         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_575879, whole genome shotgun sequence.
ACCESSION   DASTEY010000682 DASTEY010000000
VERSION     DASTEY010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575879"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /locus_tag="VFO72_11875"
     CDS             <1..2048
                     /locus_tag="VFO72_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET9480040.1"
                     /translation="DGIAVGMATKIPPHNLTEVVDATLALLKDPELKVEDLTKIVTGP
                     DFPTGGFIYGREEIRKSYVDGRGVLQLRARAGIDRIGRGAQERDAIVITEIPFQVNKA
                     RLIERIAELINDKKLDGISDLRDESDRSGMRIVVELKRDAVPQVVLNKLYKLTPMQSS
                     FGVINLAIVNGQPRVLNLKQMLEAFIEFRREVVRKRTEYELRKAKARAHILEGLTKAI
                     DALDYIVTLIRNSRSVDEARQWLMGQLKTTSEVKTWKGVPSDVQLKTYLTRLQKTMES
                     LSFSELQAQAILDLQLRRLSALERQKIIDEYESVIKLIAELEEILSNERSLRRVIAKE
                     LEEVRKDFGDERRTEIVDEGVELSIEDLIADEDVAITVTNSGYIKRTPITTYQRQGRG
                     GKGRFGAAAKNGDFVEHLFIASTHAYLMIFTDDGMVYKLKVHEVPDAAASARGKAVVN
                     LINIPSDRRLAGVVPVREFSEGRYVVMVTRKGVIKKTSLLDFQNIRSNGIIAINIDEG
                     DRLLDVVLTDGTKRIFIATHNGLAIRFDEKNARPMGRATRGPRGIELRKDDFVVSVCA
                     VSAEDKERMLSVSEQGYGKQTPITTYRLQARGGKGVINMKTTAKTGKVVAVFPVEDDA
                     EIMIITQQGKLIRLEAADIRKTGRSAQGVRLIKTDAGDKVTSASLVEADEETEDAPA"
     gene            complement(2066..2767)
                     /locus_tag="VFO72_11880"
     CDS             complement(2066..2767)
                     /locus_tag="VFO72_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET9480041.1"
                     /translation="MITIRVLFFGAARDVVDQNPFPLSLAADGPPTVARAFQQLVQQF
                     SGLERFGRSLLFAVNQEYATAETELRENDELAIFPPVSGGASSHDFFELTTEPIDVGS
                     VARRVVLPECGATVTLDGFAREWTAGRRTKYLVYEAYDAMALSEMQRLGAEAHTRFDI
                     AHIGIVHRTGKLEIGETSVVISVSAPHRKAAFEACEWAIRELKRTVPIWKKEVYDVGE
                     QWVTTDNTDFTEPSV"
     gene            complement(2764..3453)
                     /locus_tag="VFO72_11885"
     CDS             complement(2764..3453)
                     /locus_tag="VFO72_11885"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9480042.1"
                     /translation="MDRVRGVSELPVFPLPVVLFPGVPLPLHIFEPRYRQMLVDVRST
                     NNLFGVSYFDPTTSTEMVPPAGNVGCVAEVTELQGLADGRSNILTIGVVRYRIEAYVE
                     RGAPYLVAKLSYFEDREEEDDALAGLTKEVADVFTRIAQAVRTINDERTNLPDISDTE
                     PQRLSFLVAAAMEIDAEIKQELLELRSTFERLERLRAMLSQAVKGYEERARIHELAKG
                     NGHGGRRIEIE"
BASE COUNT          892 a         1061 c         1075 g          768 t
ORIGIN      
        1 ccgacggcat cgcggttggc atggcgacca agatcccacc gcacaacctg acagaggtcg
       61 tcgacgccac gctggcgttg ctgaaagatc cggaactgaa agtcgaagac ttaaccaaga
      121 tcgtaactgg tccagacttt cccaccggcg gctttatcta cggacgcgaa gagatcagaa
      181 agtcgtacgt cgacggccgc ggcgttttgc agttgcgcgc tcgcgccggc atcgatcgga
      241 tcggacgtgg agcgcaggag cgcgacgcga ttgtgattac tgaaataccg tttcaggtca
      301 acaaagcgcg gctcatcgaa cgcatcgccg aactcatcaa cgataagaag ctggacggca
      361 tctccgatct gcgcgacgaa tccgatcgca gcggcatgcg catcgtcgtg gaattgaaac
      421 gcgacgcagt gccgcaggtc gtgcttaaca agctctacaa actcacaccg atgcaatcgt
      481 ctttcggcgt gatcaatctc gctatcgtca acggccagcc gcgcgtgctc aatctcaagc
      541 agatgctcga ggcgtttatc gagtttcgcc gtgaagtagt gcgcaagcga accgaatacg
      601 agctgcgcaa agcgaaggca cgagcgcaca ttctcgaagg gctcaccaaa gcgatcgacg
      661 cgctcgatta catcgttacg ctgattcgca actcgcgttc agtggacgaa gcccgccagt
      721 ggttgatggg ccaacttaaa accacgagcg aggtgaaaac gtggaaaggc gttccgtctg
      781 acgttcaact caagacttac cttacgcggt tgcagaagac gatggaaagc ctgtcgtttt
      841 ccgagcttca ggcacaggcg attctcgacc tgcaattgcg tcgcttgtcg gctctcgaac
      901 ggcaaaagat catcgatgag tacgagagcg tcatcaagct gatcgctgag ctggaagaga
      961 ttctcagcaa tgaacgatcg ctgcgccgcg tcattgcgaa ggagctcgaa gaggtcagaa
     1021 aagattttgg cgatgagcgg cgaaccgaga ttgtcgacga gggtgtcgaa ctctcgattg
     1081 aagatctcat cgcagacgaa gacgtcgcca tcaccgttac caacagcggt tacatcaaac
     1141 ggacgccgat cacgacttat caacgtcagg gacgcggcgg caaagggcgt ttcggcgcgg
     1201 ccgcgaagaa cggcgacttc gtcgaacatc tctttatcgc ctcaacccat gcctacctga
     1261 tgatcttcac ggacgatggc atggtctata agttgaaagt tcacgaagtg cccgatgcgg
     1321 cggcgtctgc ccgtggcaag gcggtcgtga atctcatcaa tattccgagc gatcggagac
     1381 ttgccggcgt ggttccggtg cgtgagtttt cagaaggccg ttacgtggtg atggtcacgc
     1441 gcaagggcgt gatcaagaag acttcgttgt tggactttca gaacatccgc agcaacggca
     1501 tcatcgcgat caatatcgac gaaggcgacc ggttgctcga cgtcgtgctc actgacggta
     1561 ccaagcgcat cttcatcgct acgcacaacg gcctcgcgat tcgtttcgac gaaaagaacg
     1621 cgcgaccgat gggccgcgct acgcggggtc cgcggggaat cgagttgcgg aaggacgatt
     1681 tcgtggtttc ggtttgcgcg gtctcggctg aagacaagga gcgaatgttg tcagtgtccg
     1741 aacagggata cggcaagcag acgccgatta cgacctatcg tttgcaagcg cgtggtggca
     1801 agggtgtcat caacatgaag actaccgcaa agacggggaa ggtcgtggct gtgtttccgg
     1861 ttgaagacga cgccgagatc atgatcatca cgcagcaggg caagctaatc aggctggagg
     1921 cggctgacat tcggaagacg ggccgcagcg cgcagggtgt ccgattgatc aagaccgacg
     1981 cgggcgacaa agtgacgagc gcgtcattag tcgaagccga cgaggaaaca gaggacgcgc
     2041 cggcataaac cacggacttc acggactaaa cagaaggttc tgtgaaatcg gtgttgtctg
     2101 tggttaccca ctgttcgcca acgtcgtaaa cttctttctt ccagataggc acggtgcgtt
     2161 tgagttccct gatggcccac tcacaggcct caaacgccgc cttgcggtgc ggcgcgctca
     2221 cggagatcac gacgctcgtt tcacctatct ctagttttcc cgttcgatgc acaatcccga
     2281 tatgcgcaat gtcgaaacgc gtgtgtgctt cggcgcccaa gcgctgcatc tccgacaagg
     2341 ccattgcatc gtacgcttcg taaaccagat acttcgtccg ccgtcccgca gtccactcgc
     2401 gcgcgaaacc atcgagcgtc accgtcgcgc cacactccgg caacaccacg cgacgcgcca
     2461 cagaaccaac atcgatcggc tccgtcgtta actcaaaaaa atcgtggctt gaggcaccgc
     2521 cactcactgg cggaaagatt gccaactcgt cgttctccct caattccgtt tcggctgtgg
     2581 cgtactcctg attgaccgcg aacaaaagcg aacgtccgaa gcgttcgagg ccgctaaact
     2641 gttggacgag ttgttgaaac gcgcgcgcaa ctgtcggtgg cccgtccgcc gcgagcgaaa
     2701 gcggaaaggg attttgatcg accacgtcgc gagcggcgcc gaaaaaaaga acgcggatag
     2761 ttatcattcg atctcgatcc ttctgccgcc gtgcccattg cctttcgcca gttcgtgaat
     2821 ccgcgctcgc tcttcgtaac ctttcaccgc ctgcgaaagc atcgctcgca gtctttccaa
     2881 ccgttcaaat gtcgagcgca actccagcag ttcctgttta atctcggcat cgatctccat
     2941 cgcggccgcg acgagaaacg aaagacgttg cggctcggta tcggaaatgt cgggcaagtt
     3001 cgttcgttcg tcgttgatcg tgcggacggc ttgtgcgatg cgcgtaaaaa cgtcggcaac
     3061 ttccttagtc aaaccagcaa gcgcatcgtc ctcttcttcg cggtcttcaa aataactgag
     3121 cttcgcgacg agatagggcg cgcctcgttc gacataagct tcaatccggt atctcaccac
     3181 accaatagtc agtatgttcg agcgcccatc ggcaagccct tgcagctccg taacttcagc
     3241 tacgcagccg acgttaccgg caggtgggac catttccgtc gaagtggtcg gatcgaaata
     3301 agagacgcca aaaagattgt ttgtggaacg aacgtcgacc agcatttgcc ggtagcgtgg
     3361 ttcaaagatg tggagcggca aaggcacgcc tggaaacagg accacaggca aaggaaacac
     3421 aggcagttcg ctgacgccgc gtactctgtc catagcttcg ctcattggtg agccgattat
     3481 ggcagattag gaggagttaa aaaaatgcag cccccgccgc gtgtgggaga cgctgcgggg
     3541 gccttcgagc acacctttcg atgaagaggg gtttcatctc cggtgctaga cgggcgcctg
     3601 gagtgggtgg cgtccggatc ttcaaagaat gtgagcaaac aatttcagcc tatgaaccgg
     3661 tatcaatctg caaatgcttg cgagcaggtc ttcaatcttg aatggcttcg aaaagaaacc
     3721 atccgcgccg gcggccatgc aagcctcacg tacgtcagat tcgctgtttg aactcattac
     3781 caggaccttc gactga
//
LOCUS       DASTEY010000683         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_394472, whole genome shotgun sequence.
ACCESSION   DASTEY010000683 DASTEY010000000
VERSION     DASTEY010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394472"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..587
                     /gene="fabZ"
                     /locus_tag="VFO72_11890"
     CDS             132..587
                     /gene="fabZ"
                     /locus_tag="VFO72_11890"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET9480043.1"
                     /translation="METVLDSQAIQKLLPHRYPFLLVDRIVELEPRERIVGIKQVSIN
                     EPFFQGHFPGAPVMPGVLVVEALAQVGAVLALREIEDRDEKLVLFTGIKEARFRRPVV
                     PGDTLVLEVRALRIGSRVQRMRGEAKVDGQLAADAEIMSIVAPREASAI"
     gene            590..1384
                     /gene="lpxA"
                     /locus_tag="VFO72_11895"
     CDS             590..1384
                     /gene="lpxA"
                     /locus_tag="VFO72_11895"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET9480044.1"
                     /translation="MSTVVHRTTIHPTAVISPNTKIGDNCVIGPYCILGSDVVLGDNV
                     RLESHCVIDGRTWIGAETHVFPFVSIGLASQDLKYKGEPSETRIGARNKIREFVTVHR
                     GTAGGGMVTKIGDDCLIMAQAHIAHDCLIGDGVIMANAATLAGHVTIEDGANIGAYSG
                     IHQFCRVGKEAYIGGYSVVVKDALPFALTVGNHAKCYGLNVTGMKRRGYPRATIDALH
                     HAFHLLLSAKLNTTQAVERIKNEITDSAEVSYVLRFIEESSRGVTK"
     gene            1381..2178
                     /gene="lpxI"
                     /locus_tag="VFO72_11900"
     CDS             1381..2178
                     /gene="lpxI"
                     /locus_tag="VFO72_11900"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET9480045.1"
                     /translation="MKFGLIAGNGKFPFMVVEGARQSGAELVVAAIREETDPEIERLA
                     DRVTWVGIGQLGRMIRFFKEEGVEKAIMAGQVKHVQIFSRAVPDARMLKVLLKLPRRN
                     TDSLIGAIASELQSEGIELIDSTYFLHESLPQSGTLTRRQPDKNEREDIEYGLEVARE
                     IARLDLGQTIVVRGKACVAIEAMEGTDATIKRAGSLVSGRLTVVKIAKPNQDMRFDVP
                     VVGLPTIEAMVEAGATCLCITAGKTLMFDRVKMIAAADKNKIAIVAV"
BASE COUNT          496 a          600 c          664 g          476 t
ORIGIN      
        1 gaaggcattg ccgattgccg attttcgatt gccgattgcc actttggcga ctgacgcttt
       61 gacgatttct gtctaacaat caacaatcaa caatcaacaa tcaacaattg gcaatcggca
      121 atcgacaatc tatggaaaca gttctcgact ctcaggcaat ccagaaactg ctgccgcatc
      181 gctatccttt tctgctggtc gaccgcatcg tcgaactgga gccacgcgaa cgcatcgtgg
      241 gcatcaagca ggtgagtatc aacgaaccgt tttttcaggg ccactttccg ggtgcgccgg
      301 tgatgccggg cgtgctggtc gtcgaagccc tggcccaggt cggcgctgtg ctggcgctac
      361 gcgagatcga ggatcgcgac gagaaactcg tactgtttac cggcatcaaa gaggcgcggt
      421 tccgccgtcc ggtagttccc ggcgacacgc tcgtgcttga agttagggct ctgcgcatcg
      481 gttcgcgcgt gcaacgtatg cgtggcgaag ccaaagtgga tgggcagctg gcggccgacg
      541 cagagatcat gagcattgtc gcgccgcgcg aagcgagtgc gatttgagta tgtctacggt
      601 tgttcatcgg accacaattc atccgactgc ggttatcagc ccgaacacga agatcgggga
      661 caactgcgtc atcggaccct actgcatcct tggcagcgat gtggttctcg gcgataacgt
      721 tcgcctcgag tctcattgcg ttatcgacgg ccgcacgtgg attggcgccg aaacacacgt
      781 cttcccgttc gtctcaatcg gtctcgcttc acaggatttg aaatacaaag gcgagccgtc
      841 agagacccga attggcgcgc ggaacaagat acgcgagttt gtcacggttc atcgcggcac
      901 tgccggcgga ggcatggtca cgaagattgg cgacgactgc ctgattatgg cccaggcaca
      961 catcgcgcac gactgcctca tcggcgatgg cgtcatcatg gccaacgccg ccactcttgc
     1021 aggacacgtc actatcgaag atggcgctaa catcggcgcc tactcgggaa tccatcagtt
     1081 ctgtcgcgtt gggaaggaag cctacatcgg cggctactca gtggtcgtga aagatgcgtt
     1141 gccgtttgct ttgacagtcg gcaatcacgc gaagtgttac ggcctcaacg taacgggcat
     1201 gaagcggcgc ggttatcctc gcgccaccat cgacgcactt catcatgctt ttcacctgtt
     1261 actctccgcg aaactcaaca ccacccaggc agtggagcga atcaagaacg agatcacgga
     1321 ttctgcggaa gtctcttacg tgcttcgctt catcgaagag tccagtcgcg gcgttaccaa
     1381 atgaaattcg gactgattgc gggcaacggc aagtttccct tcatggtcgt tgaaggcgcc
     1441 cggcaatccg gcgccgagct tgtcgtcgca gcgattcggg aagagaccga tccggagatc
     1501 gagcggctgg cggatcgagt gacgtgggtg ggcatcggcc aactcggcag gatgatccgt
     1561 ttctttaagg aagaaggcgt cgagaaagcg atcatggcgg gacaggtgaa gcacgtgcag
     1621 atcttcagtc gcgccgtgcc tgacgcgcgc atgttgaaag tgttgcttaa attgccgcga
     1681 cgcaatacgg attcactgat tggcgccatc gcttcagagt tgcagagtga aggcatcgag
     1741 ctgatcgact caacttactt tcttcacgag agcctgccgc aaagcggcac gctgacgcgc
     1801 cgccagccag ataaaaacga acgcgaagac attgaatacg gtttggaagt cgcgcgcgaa
     1861 attgcccggc tcgacctggg ccagacgatt gttgtgcgcg gtaaagcttg cgtggcgatt
     1921 gaagcgatgg aaggaacgga cgcgacgatt aagcgagctg gttcactggt gtcggggcgg
     1981 ctgaccgtcg tgaagatcgc caaaccgaat caggatatgc gctttgatgt accggtggtt
     2041 ggtttgccga caatcgaagc aatggtcgaa gccggcgcga cgtgtttatg catcactgcc
     2101 ggcaagacgt tgatgttcga tcgggtaaag atgattgcgg ctgctgacaa gaacaagatc
     2161 gcaatcgtag cggtttaacc actgattacc tgacttcagc agtggattcc gctttcggat
     2221 cgtagggcgt agcgag
//
LOCUS       DASTEY010000684         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_938679, whole genome shotgun sequence.
ACCESSION   DASTEY010000684 DASTEY010000000
VERSION     DASTEY010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938679"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..877
                     /locus_tag="VFO72_11905"
     CDS             59..877
                     /locus_tag="VFO72_11905"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET9480046.1"
                     /translation="MRYVIQRFGELHDSGCFVLPNPWDAGSAAYLRHLGFKALATTSS
                     GFAFSRGMPDDVRYVRRDAVLEHFREIVNATPLPVNGDFQNGHADEPEFVAENVKLCI
                     DTGVAGLSIEDSTGDERKPLYDLELAIERVKAARRAIDESGVPVVLTARCEAWLVDER
                     DPFATSIKRLTAYADAGAECLYAPGVTDLAEITAIVQAVSPKPVNVLVSRPDANLTVA
                     KLADLGVRRISVGSALARVAWGAFIRAAREIADNGTFEAFGDSVSGVELNNLFR"
     gene            968..1294
                     /locus_tag="VFO72_11910"
     CDS             968..1294
                     /locus_tag="VFO72_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9480047.1"
                     /translation="MPTIPQRDLYRMINKAGERGERVVVATVAHTRGSTPQQRGAKMV
                     FFQNGEVAGTVGGGCIEAEVWAEAQTAMRSGHSALHHFSLTADEASEEGMVCGGTMDI
                     FVEVIK"
     gene            1394..1687
                     /locus_tag="VFO72_11915"
     CDS             1394..1687
                     /locus_tag="VFO72_11915"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HET9480048.1"
                     /translation="MPNLLANYKAKHQHPLNRLSHSIGIPLIVISLPVFFFNWRWALA
                     LFVGGWIFQFIGHAIEGNQPAFFNNPVYLLVGPLWLLRRVVALLGLSKTTPAK"
     gene            1709..>2051
                     /locus_tag="VFO72_11920"
     CDS             1709..>2051
                     /locus_tag="VFO72_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480049.1"
                     /translation="MPENSEQPSSLSITKKIAELLERGAVGVVTTVARGSNPGAKLLF
                     DEASNTVGTLGNLELDSLVVPQAAKFLQSRDDTRLVCVKDFATDFPAATEIYLLFERL
                     QPSPRLVVCGAG"
BASE COUNT          441 a          562 c          582 g          466 t
ORIGIN      
        1 cacagaacca cgctcgacat aagtgtcttc atatattcct ttctgttacg attcgaccat
       61 gcgatacgtc attcagagat tcggggagtt acacgactcc ggctgtttcg tactgccaaa
      121 cccgtgggac gccggcagtg cagcttacct tcgtcacctc ggtttcaaag cactcgccac
      181 cacctcgtca ggtttcgcgt ttagtcgcgg catgccggat gacgttcgct acgttcggcg
      241 cgacgcggtg ctcgagcact ttcgggaaat tgtaaacgcc acgccgttgc ccgtgaatgg
      301 cgatttccaa aacggccatg cagatgagcc cgagtttgtc gcggagaacg tcaagctttg
      361 catcgacacg ggcgttgccg ggctctcaat tgaagactcc actggcgatg agcgcaaacc
      421 gctctacgat cttgaactcg cgattgaaag agtcaaggct gcgcgccgcg cgatcgacga
      481 gtcgggcgtt ccggttgtgc taactgcgcg atgcgaggcg tggctcgttg acgagagaga
      541 tcccttcgcg acgtcgatca aacggttgac ggcgtacgct gatgccggcg ccgagtgtct
      601 ttacgcgccg ggcgtaaccg atctcgcaga gattactgcg atcgtgcaag ccgtttcacc
      661 aaaaccggta aacgttcttg tgtcgcgacc ggatgcgaac ctcactgttg caaaactggc
      721 cgatctcggt gtgcgtcgca tttccgtcgg atcagcactg gcgcgagtcg cgtggggtgc
      781 gttcattcga gctgcacgcg agatcgctga taacggcacg ttcgaggcct tcggcgacag
      841 cgtatccgga gttgaactga acaacctgtt tcgctaggcg gagaggctta ctcttcagcc
      901 gtggcaaaca ttcttgctgg tgacgtaaac tatctcaccg aaaggcaaaa cacaaagcac
      961 aagcacgatg ccgacgattc cacaacgcga cctctatcgc atgatcaaca aagccggcga
     1021 acgcggcgaa cgcgtcgtag tggcgacggt ggcccacacg cgcggttcaa cgccacagca
     1081 acgcggcgcg aagatggtct tttttcaaaa cggcgaggtg gcgggaactg tcggtggtgg
     1141 ttgcatcgaa gcggaagttt gggccgaggc gcaaacagcc atgcgttccg gccattcggc
     1201 gttgcaccac ttctcgctga cggctgatga agccagcgaa gaaggaatgg tttgcggcgg
     1261 cacgatggat atctttgtcg aggtgattaa gtgattgtca attgccgatt gccgattgcc
     1321 gatttgaaag gcaaggcgtt taagtcttga gatcggcaat tacaaatcgg caatcgaaaa
     1381 tcggaaatcg acaatgccaa acctcttagc caactacaaa gccaagcatc agcatccgct
     1441 gaaccggctg tcgcacagca tcgggattcc cttgatcgtt atctcgctgc cggtcttttt
     1501 cttcaactgg cgctgggcgt tggcgctgtt cgtcggcggc tggatctttc agttcatcgg
     1561 tcacgcaatc gaaggtaatc aacccgcttt cttcaataac cctgtctatc tcctggttgg
     1621 accactgtgg ctgctgcggc gagtcgtggc cttgctgggc ttgagcaaaa cgacacctgc
     1681 aaaatagcaa ctcgtcacat cattcttcat gccggaaaac agtgagcaac catcttcgct
     1741 aagcatcacg aagaaaattg ctgagttact cgaacgcggc gcggtcggtg ttgtgactac
     1801 ggtagcgcgc ggatcaaacc cgggcgcgaa gcttctgttt gacgaagcgt cgaatactgt
     1861 tggaactctg ggaaatttgg agttggattc gctagtcgtg ccgcaggctg cgaagtttct
     1921 tcagtcacgc gacgacactc gcctggtctg cgtcaaggat ttcgctactg attttcctgc
     1981 cgccactgag atctatcttt tattcgaacg gttgcagccg tcgccgcgac tggtcgtttg
     2041 tggcgccgga c
//
LOCUS       DASTEY010000685         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_444251, whole genome shotgun sequence.
ACCESSION   DASTEY010000685 DASTEY010000000
VERSION     DASTEY010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444251"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1047)
                     /locus_tag="VFO72_11925"
     CDS             complement(4..1047)
                     /locus_tag="VFO72_11925"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9480050.1"
                     /translation="MPNDVLTEKRTGSRRFAPLGIIFGVVGLLLFAYFVSRAGIGEIF
                     YRIRLLGAGFLIILAVSSIRYIVRALAWTRCIEHPHRLPFRDAFAARIMGDALGNITP
                     FISFAVSEPSKAVFVKDRVPLLVGLSGLAIENIFYALSVAVFIFTGTATLLLTFSLPK
                     PLRYASIGALTATVIIIPLVYLLIRSQARFISGALGFLQHRGIARKLMERLKPRARSL
                     EDRVYGFYANSQRSFISIFALDCCFHLAGVVEVFITLSFISPVPPTLTQAFILESVNR
                     VINVAFKFIPLRAGVDEGGTGQVSKVLGFARGIGEALAIVRKGRDIFWSAVGLILIWK
                     RGHRLTQMIADQR"
     gene            1190..>2049
                     /locus_tag="VFO72_11930"
     CDS             1190..>2049
                     /locus_tag="VFO72_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9480051.1"
                     /translation="MSQSHPAIAPLSGTPLTTLSEDETMFRASVREFAEGELRPRVEQ
                     MDEAAKLDPAIIKQCFELGLMGIETPEEYGGAGATFFTAILAVEELSRVDASVGVFVD
                     VQNTLVNNAFIRWGTPDQKKNYLTRLASDHVGAYALSEPSSGSDAFGMQTRAVEKDDH
                     FLLNGRKLWITNGIEAEIFVLFANANPEAGYRGITAFIVEKSFPGFSIGKKENKLGIR
                     ASSTVELILEDCKVPKENVLGEAGKGYKISIETLNEGRIGIGAQMIGIAQGALESALA
                     YTAERQQFGKT"
BASE COUNT          531 a          583 c          546 g          389 t
ORIGIN      
        1 agattaacgc tgatctgcga tcatctgcgt taatctgtgg cctctcttcc aaatcaatat
       61 tagccccaca gcgctccaga atatatcgcg gccctttctc acgatcgcca gcgcctcgcc
      121 gatcccgcgc gcgaaaccaa gcaccttgga cacctggccc gtgcctcctt catcgacgcc
      181 cgcacgcagc ggaataaact tgaacgctac gttgatcact cgattcactg attcgagaat
      241 gaaagcctgc gtcaaagtcg gcggcaccgg actgatgaac gaaagggtta tgaatacctc
      301 gaccacaccc gcgaggtgaa aacaacagtc caacgcaaag atcgagatga agctgcgttg
      361 gctgttcgcg taaaacccgt agacgcggtc ttcgagagat cgcgctcgcg gtttcaatct
      421 ttccattaac ttccgcgcga tgccgcgatg ttgtagaaag cccaacgcgc cactaataaa
      481 tctcgcctga ctacgaatca gcaggtacac gagcgggatg ataatgactg tagctgtaag
      541 cgcgccgatg ctggcataac gcaaaggctt cggcagtgaa aaggtcagca gcagagtcgc
      601 ggtaccggta aagatgaaga ccgcgaccga caacgcatag aagatattct caatcgccag
      661 tcctgacagt ccgaccagca gaggcacacg atcttttaca aagaccgcct tcgagggctc
      721 gctgaccgcg aagcttatga acggagtgat attgccgagc gcgtcgccca tgatgcgggc
      781 agcgaaagca tcgcgaaacg gtaaccggtg tggatgttcg atacagcgag tccaggcgag
      841 cgcgcgaacg atgtaacgaa tactcgaaac cgcgagaatg atgagaaagc ccgcgcccag
      901 gagccgaatt ctataaaaga tctcgccaat gcccgcccgg ctgacaaagt aggcaaacaa
      961 aagcaggccc acgacaccga agatgattcc aagtggcgca aatctcctgc tcccagtgcg
     1021 cttctcagtt aaaacatcgt tcggcatcga agtacggtac cacacggcgc gaaatcggcg
     1081 gcaaccggta tgccgccgct aaaagtgacc cactgtcttt gtgattaaac ggtctctttg
     1141 attatgatgc tacgttctat tctataaagg taaaacgagc actaatacaa tgagtcaatc
     1201 ccacccagct atcgcgccgc tatcaggcac tccactaacc acgctctcag aagacgagac
     1261 aatgtttcgc gccagcgtgc gcgagttcgc ggagggggag ttgcgcccgc gcgtggagca
     1321 aatggatgag gccgcgaagc tcgatccggc aatcatcaag cagtgttttg aactgggatt
     1381 aatgggcatt gagactcccg aggagtatgg cggcgccggc gcgactttct tcaccgccat
     1441 tctcgcagtg gaagaacttt cgcgggtcga cgcgtcagtt ggagtttttg ttgacgtgca
     1501 gaatacgctc gtcaacaacg ccttcatccg ttggggcact cccgatcaga aaaagaatta
     1561 cctgacccgg ctcgcctccg atcacgtcgg cgcctacgcg ctcagcgagc ccagttccgg
     1621 ctctgacgca ttcggcatgc aaacgcgcgc ggttgaaaag gacgatcact ttctcctcaa
     1681 cggccgcaag ctctggatca caaacggcat cgaagccgag atctttgtcc tcttcgccaa
     1741 cgcgaatccg gaagcgggct atcgcggcat caccgcgttc atcgtcgaaa agagttttcc
     1801 gggcttctcg atcggcaaga aagaaaacaa actcggtatt cgagcgtcgt caacagtcga
     1861 actgatcctc gaagactgca aggtgccgaa agagaatgtg ctcggcgaag cgggcaaggg
     1921 ctacaagatc tcgatcgaaa cactgaacga aggtcgcatc ggcataggcg cacagatgat
     1981 cggcatcgcc cagggcgcgt tggaaagcgc acttgcttac acggcggaac gacaacagtt
     2041 cggcaagac
//
LOCUS       DASTEY010000686         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_114426, whole genome shotgun sequence.
ACCESSION   DASTEY010000686 DASTEY010000000
VERSION     DASTEY010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114426"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VFO72_11935"
     CDS             <1..1375
                     /locus_tag="VFO72_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9480052.1"
                     /translation="YPGNANSLFAEDRALFTMTFRYPKQYMFVGTGAPVEPDSRDGDA
                     SVAKWSSGKTELAVAGFNYGRFKRKEVNDKDSGYNVEFYANLEVPDELKQVQRNIEQA
                     ESQGVRTMTTLGSISTTAMADPAIADAQNSMRIYNMFFGKLPYTRIAMTQQPAAGFGQ
                     AWPTLVYMPYLAYVDTTQRSQLLGTRGGTDTFWRYVAPHEVAHQWWGHIIGWNSYRDQ
                     WMSEGFAEFSASLYVQLVRGNDKFYDFWEDLRQQIVQATPATRGRKPYTIGPVTQGYR
                     LNNAKTGGAARFLIYPKGAYILHMLRMMMYKQGKGGDAAFQAMMKDFVQTHYNQAVST
                     EDFKRAVEKHMTKEMDIDKNGKMDWFFDEWVYGMEVPAYKFEYSVSKDGMLTGKLTQS
                     GVSDKFVMLVPIYVDMGKGWAKLGSATMVGSGSVDIKDLKLPAVPKRMAVCAMNDVLA
                     TSIENSK"
     gene            complement(1572..>2195)
                     /locus_tag="VFO72_11940"
     CDS             complement(1572..>2195)
                     /locus_tag="VFO72_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480053.1"
                     /translation="WHARLDTHCLEGGKTTDTPRRKLFKILQKKSEQFAIIEQKSDRR
                     TAFFNWLDTLRISLDLNEAAWLLEATKAFLERKEPKQDWTEMFNGLRSVRVSSRLSEG
                     WERDGTRLFLSPPLYNESLLVQYLLSRSHEHGGRTVEGRLTLLELLRRMRKGGYLSAH
                     GIEATDQFELFEKLIDEVAGSGSVKLYYVVDRREFIDKVKSVYSSYA"
BASE COUNT          525 a          616 c          594 g          460 t
ORIGIN      
        1 gtatcccggc aatgccaact cgcttttcgc tgaagaccgg gcgctgttca ccatgacttt
       61 ccggtatcca aagcagtaca tgtttgtcgg taccggcgcg ccggtcgaac ctgactcgcg
      121 cgacggcgat gcgtcagtgg ccaagtggtc cagcggcaaa actgaattgg cggttgccgg
      181 tttcaactac ggccggttca agcgcaagga agtcaacgac aaagactccg gttacaacgt
      241 cgagttttat gcgaacctgg aagttcccga tgagctgaaa caagtccagc gcaacatcga
      301 acaagccgaa agtcagggcg ttagaacgat gacgacgctg ggttcgatct ccaccacggc
      361 gatggctgac ccggcaattg ccgacgcaca aaactcgatg cgcatctaca acatgttttt
      421 cggcaaactg ccgtatacgc gtatcgccat gactcaacag ccggcggccg gcttcggcca
      481 agcgtggccc acgctggtct atatgccgta tctcgcgtac gtcgatacca cccagcgttc
      541 tcagttatta ggtacgcgtg gagggacaga tactttctgg cgctacgttg caccgcacga
      601 agtggcgcat caatggtggg gacacatcat cggctggaac agttatcgcg accagtggat
      661 gagcgaaggc tttgcagaat tttcggcgtc gttatacgtg caactggttc gcggcaacga
      721 taagttttac gatttttggg aagacctgcg gcagcagatc gtgcaagcga cgccggcaac
      781 aagaggtcgc aagccttaca cgattggtcc ggtgacgcaa ggttaccggc ttaacaacgc
      841 caaaacgggc ggcgccgcga gattcctgat ctatccgaaa ggcgcctaca tcctccacat
      901 gctgcgcatg atgatgtaca agcagggcaa aggcggcgac gcagcgtttc aggccatgat
      961 gaaagacttc gtccaaactc attacaacca ggccgtctcg accgaggatt tcaaacgcgc
     1021 cgtagagaag cacatgacga aggagatgga catcgacaag aacggcaaga tggactggtt
     1081 cttcgatgaa tgggtctacg gcatggaggt cccggcgtat aagtttgaat acagtgtcag
     1141 caaggacggt atgctgaccg gaaagctcac gcagtcagga gtgtccgaca agttcgtgat
     1201 gctcgtaccg atctatgtcg acatgggtaa gggctgggcg aaacttggct cagcgacaat
     1261 ggtgggtagc ggatctgtgg acatcaaaga tctcaagctg ccggctgtgc caaaacggat
     1321 ggcagtatgt gcaatgaacg acgtgctcgc caccagtatc gagaacagta agtaacagtc
     1381 ggaaccacgg agaccacgga cgtctccttc atgtcaaggg cgtctccttc gtcaaggggg
     1441 tctctttcct ggggaccgca cgcgtctcgc ctgctgttta cgcaacgagt actctgcgca
     1501 agaatgcagg cgacacgcct gcggtcccag gaaagagacc cccgcagtca ctggtaaata
     1561 tttaccagcg tttacgcgta gcttgagtaa actgatttca ctttgtcgat gaactcgcgt
     1621 cgatcgacga cgtagtagag cttcaccgat cctgaacccg caacttcatc gatcagtttc
     1681 tcaaacagct caaactgatc agttgcttcg atgccgtgtg cgctcaaata accacctttg
     1741 cgcatacggc gtagcaactc caacaacgtc aaccttcctt caaccgtgcg cccgccatgc
     1801 tcgtgggacc tgctcagcag atactggacc agcaaactct cgttatagag cggcggcgaa
     1861 agaaagagcc gcgttccgtc acgttcccaa ccttcactga gccgcgatga aacacgcacc
     1921 gaacgcagtc cgttaaacat ctccgtccaa tcctgcttcg gctcctttcg ttcgagaaac
     1981 gccttcgtcg cttccagcag ccacgccgct tcattcagat cgagcgagat tcgcaaagtg
     2041 tcgagccagt tgaaaaacgc cgtgcgccga tcggatttct gctcgatgat cgcaaactgc
     2101 tcgctcttct tttgcaggat cttgaacaac tttcgccgtg gcgtgtcggt cgttttgccg
     2161 ccttcgagac aatgcgtatc cagccgcgca tgcca
//
LOCUS       DASTEY010000687         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_608672, whole genome shotgun sequence.
ACCESSION   DASTEY010000687 DASTEY010000000
VERSION     DASTEY010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608672"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFO72_11945"
     CDS             complement(<1..836)
                     /locus_tag="VFO72_11945"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET9480054.1"
                     /translation="MTQRYAEITTEGIKYAGSKLKLLPHILRLADKVQPQTVFDGFSG
                     TTRVSQALAHDGYRVIANDIAVWSRVFGECYLLSPYPREHYEDLIDHLNAIPGKHGWF
                     TEHYGIRANGQKRPWQLHNTLKLDAIREEIERLLLNKHQRAVALTSLILAMDEVDSTI
                     GHYASYLNEWSPRSYRQMKLRIPKIIPKTREHEIHHGDIFNIAPSIDADLAYYDPPYG
                     SNNDKMPPSRVRYAAYYHLWSSICLFDNPPLFGKANRRADTSDALSASVFEDFRRNES
                     GR"
     gene            888..>2116
                     /locus_tag="VFO72_11950"
     CDS             888..>2116
                     /locus_tag="VFO72_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET9480055.1"
                     /translation="MKKTKTATSRRKFLKVLPALGAAGLSASKMPLDVLAQTATPSPS
                     PSPTPTPRITKEMMHQAEKLIGIELTDAQEAMALGGVNRNLDSYETIRKIDVPLDTEP
                     AIAFHPTRARKELYVNKTKFRFGKVEVPQFKTVEDLAFATVPQLAELIRTRRVSSTEL
                     TKMYLSRLKKFGPKLLCVVTLTEELALKQAAAADAELKRGKYRGPLHGIPWGAKDLFA
                     TKGIKTTWGAEPYRDQVIDYDSTVVERLNEAGAVLVAKLSMGALAQGARWFAGVTRNP
                     WAPDEAQQGSSGSSAGPAAATAAGLVGFSIGTETLGSIVSPSSRCGVTGLRPTYGRVS
                     RYGAMGLSWTMDKIGPMCRGIEDCAAALHAIYGPDGKDITVGDAPFNWNPDTNISSLR
                     IGYLKTEFDGPTTPPTN"
BASE COUNT          454 a          564 c          631 g          467 t
ORIGIN      
        1 aaacgtcctg attcgtttcg tctgaaatct tcaaacacgg aggcgctcag cgcgtcgctc
       61 gtgtcggccc gccggtttgc ttttccgaag agcggtgggt tatcgaaaag gcatatcgag
      121 ctccaaagat gataataagc cgcatagcgc acgcgtgaag gcggcatctt gtcgttgttc
      181 gaaccgtatg gcggatcgta ataggcgaga tccgcatcga tcgacggagc gatgttgaag
      241 atgtcgccgt gatgaatctc gtgctcgcgc gttttcggaa tgatcttcgg aatccgcagc
      301 ttcatctgcc ggtaagaacg cggagaccac tcgttcaggt aggaagcgta gtgaccgatc
      361 gtgctgtcga cttcatccat cgccaggatc aggcttgtta gtgcgacagc tcgttgatgt
      421 ttgtttagga gtaatcgctc gatctcttcg cggatcgcat cgagcttgag cgtgttgtga
      481 agttgccacg gtctcttctg tccgttggct ctaatgccgt agtgttccgt gaaccagccg
      541 tgttttcccg gaatcgcgtt caggtggtcg attaagtctt cgtaatgctc gcgtggatac
      601 gggctcagca ggtagcactc accgaagacc ctggaccaga cggcgatgtc gtttgcgatt
      661 acgcgatatc cgtcgtgcgc taacgcttgc gatacgcgcg tggttcccga gaagccgtcg
      721 aagacggtct gtggttgcac cttgtcggcg agacggagaa tgtgaggcag aagctttaat
      781 tttgaccctg catacttgat gccttcagtg gtaatctctg cgtacctctg cgtcattggc
      841 ggaagatcta accacaaaga cacgaagtca caaatgtttt tacgactatg aagaaaacta
      901 aaactgcgac aagtagacga aagtttctca aagtgcttcc ggcgcttggt gccgccggtt
      961 tatcggcttc gaagatgccg cttgatgttt tggcgcagac tgcgacgcct tcgcccagtc
     1021 cgtctccaac tccaacgccg cgcatcacca aagagatgat gcaccaggcg gaaaagctga
     1081 tcggaatcga gctcactgac gcgcaggagg caatggccct cggtggcgtc aatcgcaacc
     1141 tcgactcgta cgagacgatc aggaagatcg acgtcccgct cgacactgaa cccgcgatcg
     1201 ccttccatcc aactcgtgcg cgaaaagagc tctacgtcaa caagacgaag tttcgtttcg
     1261 gcaaagttga agtgccgcag tttaagacgg tcgaggatct tgccttcgcg acggtgccgc
     1321 aacttgccga gttgattcgc acgcgccgag tttcgtcgac tgaattgacg aagatgtatc
     1381 tctcgcggct gaagaagttt gggccaaagc tgctttgtgt tgtcacgctc accgaggagc
     1441 tcgcgttgaa gcaggcggct gcggcagacg cggagttaaa gcgcgggaaa tatcgtggcc
     1501 cactgcacgg cattccgtgg ggcgcgaaag atctcttcgc gactaaagga atcaagacga
     1561 cctggggcgc cgagccttat cgcgatcagg tgatcgatta cgactcgact gtggtcgaac
     1621 gattgaacga ggccggggct gtgctggtag cgaagttatc gatgggcgcg ctcgcacagg
     1681 gagcacgctg gttcgccggc gtcacgcgca acccctgggc gcctgacgaa gctcaacaag
     1741 gatcgagcgg ttcatccgcg ggaccggctg cggctacggc agcaggactg gtgggattct
     1801 cgatcggtac ggaaacgctt ggctccatcg tttcgccgtc gtcacgatgc ggcgtgaccg
     1861 gtttgcgccc gacgtacggc cgcgtgagtc gttacggcgc gatggggttg agttggacca
     1921 tggataagat cggtccgatg tgccgcggca ttgaagattg tgccgcggcg ctccacgcga
     1981 tttacggtcc tgacgggaaa gacatcaccg tcggcgacgc gcctttcaac tggaacccgg
     2041 atacaaacat cagctcgctg cgaatcggtt atctgaaaac cgagtttgat ggtccaacaa
     2101 cgccgcccac aaacga
//
LOCUS       DASTEY010000688         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1285028, whole genome shotgun sequence.
ACCESSION   DASTEY010000688 DASTEY010000000
VERSION     DASTEY010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285028"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFO72_11955"
     CDS             complement(<1..416)
                     /locus_tag="VFO72_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480056.1"
                     /translation="MQKTLTLTRVFQQDGPRGVFALMLQKLLMTWRRGEVWELGKFIG
                     RSSRIARLDGCKFTIDPAVVPANVTYLLLTNQYEQPEREALNRFLDRDVPVVELGACA
                     GVISCLTNRRLTDPKRHVVVEANPAMQPVLEENRKH"
     gene            complement(446..1972)
                     /locus_tag="VFO72_11960"
     CDS             complement(446..1972)
                     /locus_tag="VFO72_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET9480057.1"
                     /translation="MAASDKSSHSLTARALWLLIAKILAFALSIALPLLLVRRLSQEN
                     FGLYKQAFLVIGTALALLPLGFQMSAFYFLPRETERRGQIILNIMLFHLVMAALGGLV
                     LLLFPQLLGTIFHSNELINYAPAMAAVIFFWVFSSYLEAVSVAHQEAKLSTIFIISSQ
                     LTKTGLLLLAAMMFGSIRALIYAALVQGILQTVILLFYLRSRFPRFWARFDWAVLRMQ
                     LSYAMPFGVAGLLFTLQMDLPNYFVSNQFGPAVYAIYAIGVFNIPLVGMLSESVGSVM
                     IPQVSYLQKHGDHREILALAGRVARKLALVYLPLYAFLIVTGREFLTVLFTSQYAASW
                     PIFAINLTLLPLSIFVADPIMRAYAEHRQFLVKLYVVLVALLFVGLWAGTSRFGLIWA
                     ISVVVVVGVIGRITMWIKIGRILHIRWRDVRLLKDVGKIAIAAAIAGLVIALVRAGLT
                     ERRPLSVLIVCAVVFGVVFVVGIVLLRVPAIEERVYLRKLFARVWPGLKTVGEPVARS
                     "
     gene            complement(2001..2651)
                     /locus_tag="VFO72_11965"
     CDS             complement(2001..2651)
                     /locus_tag="VFO72_11965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9480058.1"
                     /translation="MNNEIQAQRSFWNSEADAFQRIYTHRKSKFANALDQVFRKDMYE
                     RFTFTIANCEPIKDRTFLDVGCGNALYSLELARRGAAKVVGIDISEVMIGVCRKSSDA
                     ENLSDRCSFLQTDLLDYKPESSFDVSFGIGLFDYISDPLPVLKKMREVSTDKVIGSFP
                     RLWTWRAPVRKARLTAKGCAVFFYSKPRLEKLLSDAGFSRWRIEKVGKLYCVVAHV"
     gene            complement(2648..>2959)
                     /locus_tag="VFO72_11970"
     CDS             complement(2648..>2959)
                     /locus_tag="VFO72_11970"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HET9480059.1"
                     /translation="KLYEFPMSVLELYGRRFPFCGGGYFRLFPYAYTRFCMRKVNEQG
                     RSVVFYLHPWELDPEQPRIKNLSASQKFRHYRNLDQTERRLSRLVDEFEFTTVREVLQ
                     L"
BASE COUNT          746 a          879 c          766 g          568 t
ORIGIN      
        1 ttgtgcttac ggttttcttc aagcaccggc tgcatcgccg gattggcttc tacgacaacg
       61 tgtcgcttcg gatcggttag ccgccggttg gtcagacacg aaatgacacc tgcacacgcg
      121 cccaactcca ccaccggcac gtcacgatcg agaaacctgt tcaaagcttc gcgctccggt
      181 tgctcgtatt ggtttgtcag cagcagatac gtgacattcg ccggcacgac tgccggatcg
      241 atcgtgaatt tacatccatc aaggcgcgcg atcctgctcg acctgccgat aaactttccg
      301 agctcccaaa cctcgcctcg gcgccacgtc atcagcaact tttgcagcat cagcgcaaac
      361 actccgcgcg ggccgtcctg ttgaaacaca cgggtcaacg ttagggtttt ctgcactgca
      421 actgacaaac gtaatctcct ccttgttaac tcctcgccac cggctcaccg accgttttca
      481 aacccggcca aacacgcgcg aacagttttc gcaaataaac tcgttcttca atcgctggta
      541 cgcggagcaa tacgatcccg acgacaaaca caacgccaaa aaccacagcg caaacaatca
      601 acacgctcaa cggccgcctc tcggtcagac ccgctcgcac caacgcaatc accagccccg
      661 caatcgccgc agcgatcgct atctttccga cgtctttcaa cagccgaacg tcacgccatc
      721 gaatatgcag aatgcgtcca atctttatcc acatggtaat gcgtccgatg acgccgacga
      781 cgacgaccac cgaaatggcc cagatcaaac caaagcggct tgtgcccgcc cacaatccga
      841 caaacagcag cgccaccaac acgacataca gcttcactag aaactgccgg tgctcggcat
      901 acgcgcgcat gatcggatcc gccacaaaga tgctcagcgg caacagcgtc aggttaattg
      961 caaagatggg ccagctcgct gcgtactgcg atgtaaacaa cacggtcaga aactcgcgcc
     1021 cggtcacgat cagaaaggcg taaagcggca agtacaccag cgccaacttg cgtgcgacac
     1081 gcccggcgag tgcgagaatc tcacgatggt cgccgtgctt ctgcaagtaa ctgacctgcg
     1141 gaatcatcac cgaaccgacc gactcgctca acatgccgac cagcggaatg ttgaaaacgc
     1201 cgatcgcgta gatcgcataa accgccggtc caaactggtt cgagacgaag taattcggca
     1261 gatccatctg caacgtaaag agcaatcccg ccacgccaaa cggcatcgca tacgacaact
     1321 gcatccgcaa cactgcccaa tcaaaacgcg cccagaagcg cggaaaacgc gaccttaaat
     1381 agaacaacag gataaccgtc tgcaggattc cctgaactag agcggcgtag atcagcgcgc
     1441 ggatcgatcc aaacatcatc gcggccagca acagcagacc ggtcttcgtc aattgcgaag
     1501 agatgatgaa gatcgtcgag agctttgctt cctgatgggc caccgagact gcttcgaggt
     1561 acgacgagaa gacccagaag aagataaccg cagccatcgc cggcgcgtag ttgatgagtt
     1621 cgttactgtg aaagatcgtt ccgaggagtt gcggaaacag cagcaggacg agtccaccca
     1681 gggcggccat cacgaggtga aacaacatga tgttgaggat gatctggccc cgtctctcag
     1741 tctcacgcgg caggaaatag aacgcgctca tctgaaagcc gagtggcaac aatgcgagcg
     1801 cggtgccgat taccagaaac gcttgtttgt agagtccgaa gttttcctgg ctcaaacgcc
     1861 tcacgaggag tagcggcaga gcgatgctga gcgcgaaggc gaggatcttc gcgatgagca
     1921 gccagagggc gcgggcagtc aaagagtgac tggatttgtc ggatgcagcc aaaaacttat
     1981 ccctcaatac ctcgccgcaa tcagacgtgt gctactacgc agtagagctt cccgaccttc
     2041 tcgattcgcc aacggctgaa accggcgtcg ctcaggagtt tttccaatcg tggtttgctg
     2101 tagaagaaga cggcgcaacc cttcgccgtg agccgtgctt tgcgcaccgg cgcgcgccac
     2161 gtccagaggc gtggaaacga tccgatcacc ttgtcagtcg atacttcgcg catcttcttc
     2221 agcacgggaa gcgggtcgct gatgtagtca aacaaaccaa tgccaaagct aacgtcgaag
     2281 gatgattcag gtttgtaatc gagtagatcc gtttggagaa aagagcagcg gtctgacagg
     2341 ttttcggcgt cactcgattt gcggcagact ccgatcatca cttcggaaat atcgatgccg
     2401 acgaccttcg cggcgccgcg acgcgccagc tccagcgagt agagtgcgtt gccgcagccg
     2461 acgtctagga aagtgcggtc tttgatcggt tcgcagttgg cgatggtgaa ggtaaaccgc
     2521 tcgtacatgt ccttgcggaa aacctgatcc agcgcattgg caaacttcga cttgcgatgc
     2581 gtgtagattc tttggaaagc atcggcctcg ctgttccaaa aacttctctg ggcctgaatc
     2641 tcgttgttca aagctgaagc acctctctaa ccgttgtaaa ttcgaactcg tcgacgagcc
     2701 ggctcaaacg ccgctcggtt tgatctaaat tgcgataatg gcggaacttc tgcgaagccg
     2761 acaaattttt tatccgcggc tgctcgggat ccagctccca tgggtgcagg tagaagacca
     2821 cggaacgccc ctgctcgttc acttttcgca tgcagaagcg agtgtacgcg tacggaaaca
     2881 ggcgaaagta gccgccgcca cagaatggga agcgccgccc gtacagttcc aggacgctca
     2941 tgggaaactc gtagagttt
//
LOCUS       DASTEY010000689         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_938819, whole genome shotgun sequence.
ACCESSION   DASTEY010000689 DASTEY010000000
VERSION     DASTEY010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938819"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1343)
                     /locus_tag="VFO72_11975"
     CDS             complement(195..1343)
                     /locus_tag="VFO72_11975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET9480060.1"
                     /translation="MELDIERILPGGYGLAHAEGKTVFVSLAAPGDRLRVRVEREQGN
                     ILFASIEEILVASPDRVEPPCPYFGRCGGCDFQQLTYEKQLTAKAGIIRDCLHRIARI
                     EHVPEIVVTPSPHDWRYRMRAMWQIDPSERTIGYYERGSRRVCDVADCAVLMPELEET
                     LERVRATDWYEFPPDLKHLDVVAGENGVSLAPAFAEFETTELELTVRGERYNYNAESF
                     FQTNPGLLEPLIEFALDEVSGEAAVDLYCGVGLFTLPLARRFERVVGIEANPAAARFA
                     RRNLQRAGLKNARVLNTGVADWIRTSSAAVDFVLLDPPRSGAESAVIRGILDFRAPRV
                     SYVSCDPATLARDLKKLMAVGYAIESLVAYDLFPQTHHVETVVRLHLN"
     gene            complement(1350..1724)
                     /locus_tag="VFO72_11980"
     CDS             complement(1350..1724)
                     /locus_tag="VFO72_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480061.1"
                     /translation="MTIRRFGVLSVAKIYGLLMFIFGLILGVLYGLFFIIFGAAMSAM
                     GDRQATAGGMSSVVLGIAMMIGIPIMYGLIGFIMGAIGALVYNALAGIIGGIKFELEG
                     VQQEYAPPPPPHQWEPQQYPAQ"
     gene            complement(2172..2888)
                     /locus_tag="VFO72_11985"
     CDS             complement(2172..2888)
                     /locus_tag="VFO72_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480062.1"
                     /translation="MNELRLNYYNYFTEIEDTFVRRRGKHLFLSPMDWALMETWKQQG
                     IPLHIVLRGIERSFDSFESKPRKRTVKSLLYCQEEVEAQYAEWTEARVGASSSDNNES
                     DKTPFSLDAISEHLQTNRDLLLQTAAARQPEDDLSEAMTRAATLLADIDQDLAAGATL
                     NTRMLEDSLTGLERLLNDSMFAVASANDLERQKNAVKEQLKPYRNHMETAVYNQTFNN
                     LLLKRLREKFAVPRLSLFYV"
     gene            complement(2869..>3170)
                     /locus_tag="VFO72_11990"
     CDS             complement(2869..>3170)
                     /locus_tag="VFO72_11990"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET9480063.1"
                     /translation="CSEALFARAAKLPRVKFEKPARVIGASTTAAMQSGIYYGYAGLV
                     DGLLEKMIAELRETPRVIATGGLAHAIAPASEYIEQVDDTLTLEGLRLVYERTKT"
BASE COUNT          742 a          964 c          831 g          633 t
ORIGIN      
        1 cacacctgca accgaaagcc tgcgcatcgc acacgcaaca accaccgcga ccgcgcaaac
       61 tgcaccggca atccacaaaa ccctcgacgt agttggaaag taatttgcgg caaagacgcc
      121 tgccgcaaag gcaaatgcaa attgagccag cggatgccgg cttagtgagg catggtgagg
      181 attcatctgt cagttcagtt taagtgtaaa cgaacaaccg tctccacgtg atgcgtctgt
      241 ggaaacagat cgtaggcgac tagcgactcg atcgcgtatc caacagccat gagtttcttc
      301 aaatcgcgcg ccaatgtagc aggatcgcac gacacgtaag aaactcgcgg cgcacgaaaa
      361 tcaagtatgc ctctgatcac tgccgactca gcacccgaac gcggcggatc cagtagaacg
      421 aaatcaaccg ctgcagaact cgtccttatc caatccgcaa caccggtatt gagcacacgc
      481 gcgtttttga gacccgcgcg ttgaagatta cgtcgcgcga accgtgcggc agccggattc
      541 gcctcgatcc caaccactcg ctcgaaccgt cgcgccagcg gcaacgtaaa cagtccaacc
      601 ccgcaataaa gatcgacagc cgcttcaccc gatacctcgt ccaaggcaaa ctcaatcaac
      661 ggttccagca aaccaggatt ggtctgaaaa aatgattccg cgttgtagtt gtaacgttcg
      721 ccacgaaccg tcagctcgag ttctgtcgtc tcaaactcgg caaatgctgg tgcaagtgac
      781 acgccattct cgcccgccac cacatccaga tgcttcaagt ccggtgggaa ctcataccaa
      841 tctgtagctc ttacgcgttc gagcgtctct tccaactccg gcatcaacac cgcgcaatca
      901 gcaacgtcac aaacgcgccg tgaaccgcgt tcgtaataac cgatcgttcg ctccgacgga
      961 tcgatctgcc acatcgcccg catgcgatag cgccaatcat gcggcgaagg cgtaacaacg
     1021 atttccggca cgtgctcgat acgcgcgatg cgatgcagac agtcgcgaat tatgccggct
     1081 ttggcggtga gctgtttttc gtaagtaagt tgctgaaaat cgcacccgcc gcagcgtccg
     1141 aagtatgggc aaggcggttc gactcgatcg ggggaagcga cgaggatctc ttcaatcgat
     1201 gcaaacagga tgtttccctg ctcgcgctcg acgcgcactc gcaaacggtc acccggcgcc
     1261 gcaagcgaaa cgaaaactgt cttgccctcc gcgtgggcca atccgtaacc gcccggtagt
     1321 atccgttcta tgtcgagttc caaagacatt tactgcgctg gatactgctg cggctcccac
     1381 tgatgcggcg gtggcggagg cgcgtattcc tgctgcacgc cttcaagctc aaacttgatg
     1441 ccgccgatga tgccggcgag cgcgttgtaa accagtgccc cgatggctcc catgatgaag
     1501 ccaataaggc cgtacatgat gggtatgccg atcatcatcg cgattcccag gacgaccgag
     1561 ctcataccgc ctgcagtcgc ctgacggtcg cccattgcgg acatcgcggc gccgaagatg
     1621 atgaagaaca agccgtagag cacgcccagg atcagcccga atatgaacat gagcagacca
     1681 taaatttttg ccacggaaag cacgccgaag cgtctgatag tcatttcagc catcacgttc
     1741 tcctttaggc ttctgtcggc ccttcgggcc tcaaatcttt aattaggtgc tccagggctg
     1801 cgcgcttcgc gcttgcccct ggccaccctc ttccgcgcct ttggcgctcg tcgacgcatc
     1861 tctgtctcag acctcaacaa tccgagtcat cagagttaaa gacctcagca ggccggcagc
     1921 aggttcttgc aaacgcgccg acgcgcgagc atatggtata cacgccaacg acgaaaagcg
     1981 ctgacgcaca agcttacggt ccaacaccag ggcgagaagc gccgaaggcg cggcataaaa
     2041 tagccagggc aagggcggag cgcagcggag cccgccgccc ctgggagcaa taaaacgcga
     2101 ccgaaagccc tgaaaaggcg acagacgtgt cacgccctgc gcttccgtgc taccactaac
     2161 aaaccgaagc gtcagacgta aaacaagctg aggcgcggga cggcgaattt ctcccgcaaa
     2221 cgcttcaaga gaagattatt gaaggtttga ttataaacag ccgtctccat gtgattgcga
     2281 taaggtttca gttgctcctt caccgcgttc ttctgacgtt caagatcgtt cgccgaggcc
     2341 acggcgaaca tcgaatcgtt cagcaagcgt tcgagcccgg ttaaagaatc ttcaagcatc
     2401 cgcgtgttca aggtcgcgcc cgcggcaagg tcctgatcga tgtcggcgag caacgtcgcg
     2461 gcgcgtgtca tcgcttcgga aagatcgtct tccggttggc gtgctgcggc ggtctggagc
     2521 agcaggtcgc ggttcgtttg caggtgctcg ctgatggcgt cgagagaaaa aggtgttttg
     2581 tctgactcat tgttgtcgct cgatgaggcg ccgacgcgcg cttcagtcca ctctgcatac
     2641 tgcgcctcca cttcctcctg gcaatacaac agcgacttta ccgtgcgttt acgcggcttc
     2701 gactcgaacg aatcaaacga tcgctcgatg ccgcgcagta cgatgtgtaa cggaattccc
     2761 tgctgtttcc acgtttccat caaggcccag tccatcggac tgagaaacag atgcttgccg
     2821 cgccggcgga caaacgtgtc ttcgatttcc gtgaagtagt tgtagtagtt aagtcttagt
     2881 tcgttcataa acgaggcgca agccctcgag cgtaagggtg tcgtccacct gctcaatgta
     2941 ttcagacgcg ggcgcaatcg catgagcgag accgccggtc gcaatcacac gcggcgtctc
     3001 ccgcagctca gcgatcattt tttccagcag gccatcaacc agtccagcat atccgtaata
     3061 gatccctgat tgcatcgcag ctgtcgtcga agcaccgatc actctcgccg gcttttcgaa
     3121 tttcacgcgc ggcagtttcg ccgcccgcgc aaagagcgct tcgctgcaag
//
LOCUS       DASTEY010000690         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1202559, whole genome shotgun sequence.
ACCESSION   DASTEY010000690 DASTEY010000000
VERSION     DASTEY010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202559"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1127
                     /locus_tag="VFO72_11995"
     CDS             162..1127
                     /locus_tag="VFO72_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET9480064.1"
                     /translation="MPRGISVHVGVNSPDPIFGATPLEGCRRDAESMRDIAVDNGFEA
                     TIFLDGAAKFDDVKAAIKDAANRMQAGDIFLFTFAGHGSFRSTVAIDENDGQDETILL
                     HDCVLLDNYLRRNLWSQFAEGVRILGIADSCHSGTVLTGSTVGLSSGVLGSNLVVAGV
                     EVPRFDESPAFDVSMNGGGAAEFQATSQPVAIGVAPAPQRGVSARRIRGFTGPERERF
                     ERARPKIHERLRKELLSGEAAKVKASLLSLAACRDNEEALDGPNHGAFTQALLDVWNE
                     GSFNGNYDDFITQIRTKFPEPSQQRPIRLPQVVENSFLQQRPFTI"
     gene            complement(1145..1759)
                     /locus_tag="VFO72_12000"
     CDS             complement(1145..1759)
                     /locus_tag="VFO72_12000"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET9480065.1"
                     /translation="MDELVAQVNHEEPPPRSNAGWVEVIAGSMFSGKSEELIRRLRRA
                     KIARQKVQVFKPELDSRFADNQIVSHSEMRHDSANSRSAAEILAKIDPDTEVVGIDEG
                     QFFDNELVEVANELARRGVRVIIAGLDQDYTGKPWEPMPQLLAIAEHITKTHAICMKC
                     GQPANYSQRTFESEERVAVGAADKYEARCRRCFVPHADAPTMNG"
     gene            complement(1773..2348)
                     /locus_tag="VFO72_12005"
     CDS             complement(1773..2348)
                     /locus_tag="VFO72_12005"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HET9480066.1"
                     /translation="MAVKTIALGSDRAAKIMAVRASVARVAEIDPDWAHVNVVARRVE
                     TSAPAMPLTDWQLMEGARERALAVRDSLRSRRLEADIYVGLEGGFHSISIASEWHTFL
                     RGWAYATDGERSAFGAAPSISVPAEIVKSVVQGRRELGAVIDEVTGGRDIRSKQGAWG
                     VLSRDLVTRSLSFELALIAAFAPFYNPKFYS"
     gene            2511..>2738
                     /locus_tag="VFO72_12010"
     CDS             2511..>2738
                     /locus_tag="VFO72_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480067.1"
                     /translation="MTIDELVYDWNTVDPSFSQPNRHIGIDDETLRDGLQSPSVCEPR
                     VEQKLELLHLMEKLGIDTADIGLPGAGGTHAA"
BASE COUNT          601 a          811 c          726 g          600 t
ORIGIN      
        1 cgcgagtatt acaagattct ccctcgcgtg cccactgacc aacagttccg tctggacgtc
       61 agaattcctg aggacgaatc ttccttaacg cggggttcta ccagaacacc ctgcatgtcg
      121 atgatcaaag gagatacgta acacttcaga aaggagagac catgccaaga ggtatctcag
      181 ttcacgtcgg agtcaattcc cctgatccta tatttggagc aacaccactt gagggttgtc
      241 ggcgagacgc cgaaagcatg cgtgacattg cagtcgacaa cggatttgag gccacaatat
      301 ttcttgatgg tgcggcaaag tttgatgacg ttaaagccgc tatcaaagac gccgcaaaca
      361 gaatgcaggc aggcgacatt tttcttttca cgtttgctgg tcacggcagt tttcgctcaa
      421 cggtcgccat cgacgaaaac gacggccagg acgaaacgat tctgctacac gattgcgtgc
      481 tgctcgacaa ttacctgcgg cgaaacctat ggtcgcaatt cgcagagggc gtgcgaatcc
      541 tgggaattgc ggacagttgc catagcggta cggtgctgac cggttcgacg gtgggtttat
      601 cgtctggagt tctcgggtct aacctggtcg tcgccggcgt tgaggttccc aggttcgacg
      661 aatcccctgc cttcgacgtt tcaatgaacg gtggcggcgc ggctgaattc caggcgacct
      721 ctcaaccagt cgctatcggc gttgcgccag caccacaacg tggagtaagc gcaaggcgca
      781 ttcgggggtt tactgggccc gaaagagagc gtttcgagag agcgcgcccg aagattcatg
      841 agcgcttgcg aaaggagcta ctgtcaggcg aggccgcgaa agtcaaagct tcgcttctta
      901 gtttagccgc atgtcgagac aacgaagaag cacttgatgg gccgaaccat ggcgcgttca
      961 cgcaagcgct gcttgacgtc tggaacgaag gttcgttcaa cggaaactac gacgacttca
     1021 tcacgcagat tagaacaaag tttccagagc cgtcccaaca acgtccgatt cggttaccgc
     1081 aggtcgtaga aaactcattt ctacagcagc gtccatttac gatctgacct cgatcggaac
     1141 ttagttaccc attcatcgtt ggtgcgtcgg cgtggggtac gaagcaacgc cggcagcgtg
     1201 cttcgtattt atcagccgcg ccgaccgcca cacgctcttc ggattcaaac gtgcgctgtg
     1261 agtaattagc cggctggcca cacttcatgc agatcgcgtg agtcttcgtg atgtgttccg
     1321 cgatggccag caattgcggc atcggttccc atggcttgcc cgtgtagtcc tgatcgagtc
     1381 cggcgatgat gacgcgtacg ccgcgacgcg ccaactcgtt cgccacctcg accagttcgt
     1441 tatcaaaaaa ctgtccttca tcgataccca cgacctctgt gtcaggatct attttcgcca
     1501 gtatctcagc cgccgaacgg ctgtttgccg agtcgtgccg catctcggag tgcgagacaa
     1561 tctgattgtc tgcaaagcgc gaatccaatt ctggtttgaa aacctgcacc ttctgccgcg
     1621 cgatcttcgc tcgtcgcaag cgacggatca gttcttccga cttgccggaa aacatcgatc
     1681 cggcaatcac ttccacccac cccgcgttcg atcgcggcgg cggctcctca tggttcactt
     1741 gtgccactag ttcgtccatt cgacgttctt cattatgaat agaacttagg attgtaaaaa
     1801 ggcgcgaagg ccgcaatcag cgctaactcg aaactcagcg agcgagttac aaggtcgcgc
     1861 gacaacacgc cccatgctcc ctgtttgctt ctgatatcgc gcccgccggt aacttcgtct
     1921 atcacggcgc ccagttcgcg ccggccttgt accacgctct tcacgatctc tgcgggcacg
     1981 gaaatcgacg gcgccgcgcc aaatgcactg cgttctccat cggtggcgta ggcccagccg
     2041 cgcaaaaacg tatgccactc actggcgatc gagatcgagt gaaaaccgcc ttcaagcccg
     2101 acgtaaatgt cggcttccaa acgccggctt ctgagcgagt cgcgcaccgc gagagcccgt
     2161 tcgcgcgctc cttccattaa ctgccagtca gtcaacggca tcgcgggcgc gctcgtctcg
     2221 acgcgccgcg ccaccacgtt gacgtgagcc caatccggat cgatttccgc cactcgcgca
     2281 acgctggcgc gcacagccat gatctttgct gcgcgatcag agccgagagc gattgtcttg
     2341 actgccacag atactcgttg attcctgccg tctgggtcca gcaaacgtgc gagctgaaat
     2401 gcgaagacgc tataacatac ggctggtgct tcgtcaaaat cgcgcttgtt tttgacctgc
     2461 ttgactttag ttttcgcggt tgttgtaatc caaaccggag gctctcaagc gtgacaatcg
     2521 acgaactagt ctacgattgg aatacggtcg acccatcatt ctcccagcca aatcgtcata
     2581 tcggcatcga cgacgaaact ctgcgcgatg gactacagtc gccgtctgtt tgcgagcctc
     2641 gcgtcgagca aaaactcgaa ctgctccacc tcatggaaaa actcggcatc gataccgccg
     2701 acatcggcct gcccggtgcg ggcggcacgc acgccgcc
//
LOCUS       DASTEY010000691         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_889788, whole genome shotgun sequence.
ACCESSION   DASTEY010000691 DASTEY010000000
VERSION     DASTEY010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889788"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFO72_12015"
     CDS             <1..451
                     /locus_tag="VFO72_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9480068.1"
                     /translation="QMKAKFPEIELVSTDQYAGATRDTAKRASENLLNRFGDEVQGIF
                     TPNESSTAGMLLALQDIGKAGQVSFVGFDVSQQFIDAINNNQLHGIVVQNPFNMGYLG
                     VRTMVDSLLGKPVQQKIDTGVMLVTKENMGTPEVQALLHPPLDQYLK"
     gene            491..1435
                     /locus_tag="VFO72_12020"
     CDS             491..1435
                     /locus_tag="VFO72_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9480069.1"
                     /translation="MQTWFNRVGPVLGLLLVIAIFSLLMDTPARFLSANNLRIVLSQT
                     VIVAIGAIGMTLIIISGGIDLAVGSTIALTGVVTALAINAGFSPATALACGILVGGIV
                     GVTNGLVITRLKVVPFIATLGMLGVARGIAKWLANQQTVNMPATWLNELLVMFPDPPW
                     LLVAPGVWIAILLAICAAIVLRNTVFGRRVFALGSNEAAARACGIATDGLKIWIYGLA
                     GLLFGLAGVMQMSRLRQGDPTVAAGSELDIIAAVVIGGGSLSGGEGSILGSIIGALIM
                     AFLRNGCQQIGWPNYIQEIIIGAIIVVAVAVDRWRSRA"
     gene            complement(1507..>2227)
                     /locus_tag="VFO72_12025"
     CDS             complement(1507..>2227)
                     /locus_tag="VFO72_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9480070.1"
                     /translation="HMLIGFEALVRWHHPEFGLVPPKEFIPVAEETGLILPIGQTVLR
                     SACRQAREWQETYPTSPPLFVSVNLSVKQFNEPGLVENIAQLLEESNLPPRCLKLEIT
                     ESVFSDNIEAAVKLLTQLRNIGVQLSIDDFGTGYSSLSYLQRFPIDTLKIDRSFVTQM
                     MENEENLAIVRTIVALAQNLGMDVVAEGVETEDQLRLLRKLECENGQGFLFSTPLGER
                     QLEQFIASCNRESEEPTALVA"
BASE COUNT          455 a          601 c          658 g          513 t
ORIGIN      
        1 acagatgaaa gcgaagtttc cggagataga gctggtatcg actgatcaat acgccggcgc
       61 gacgcgtgat accgcaaagc gagcgtcgga aaatcttttg aatcgctttg gcgacgaagt
      121 gcaggggatc tttacgccga atgagtcgtc caccgcgggc atgctgctgg cgcttcagga
      181 catcggcaag gccggacagg tgtcgtttgt aggcttcgac gtgagtcagc agtttatcga
      241 cgcgatcaac aacaaccagt tgcacggcat cgtggtgcag aatccattca acatgggtta
      301 cctcggcgtg cgcacgatgg tcgacagtct gctcggcaag ccggtgcagc agaagatcga
      361 tacgggcgtg atgttggtga cgaaagaaaa catgggcacg cctgaagtcc aggcgctgct
      421 gcatccgccg ctcgatcagt atttgaaatg agccacggat ttcacggaac aacacagagt
      481 gatccatgtt atgcagacgt ggtttaatcg cgtcgggccg gtgcttggtc tgttactggt
      541 gatcgcgatt ttttcgttgc tgatggacac gcccgcgcgg tttctctcag caaataacct
      601 gcgcatcgtt ctctcgcaaa cagtcattgt ggcgatcggc gccatcggca tgacgctgat
      661 tattatcagc ggcggcatcg atctggcggt gggatcgacc atcgctttga ccggcgtggt
      721 tacggcgcta gcaatcaacg ccggtttttc tccggcaaca gctctggcat gcgggatctt
      781 agtaggcggc atcgtcggcg ttacgaatgg tctggtgatc acgcgtctga aagtggtccc
      841 attcatcgcc acgctcggaa tgctcggcgt tgcgcgtggc attgccaagt ggctggcaaa
      901 ccagcaaacg gtgaacatgc cggcgacgtg gttgaacgag ttgctggtga tgttccccga
      961 tccgccgtgg ctactcgttg ctcccggcgt ttggatcgcg attctgctcg cgatctgcgc
     1021 tgcaatcgtt ttgcgaaaca cggtcttcgg ccgccgcgtc tttgcgctcg gctcaaacga
     1081 agcggcagcg cgcgcgtgtg gcattgctac cgatggttta aagatctgga tttacggcct
     1141 ggccggactg ctcttcgggt tggcgggcgt gatgcagatg tcgcggttgc gacagggaga
     1201 tccaacggtg gcggccggct cggagttgga catcatcgcg gctgtcgtaa ttggtggcgg
     1261 aagtttgagt ggtggcgagg gcagcatact cggctcgatc atcggcgctt tgatcatggc
     1321 gtttttgcgc aacggttgcc agcagatcgg ttggcccaac tacattcagg agatcatcat
     1381 cggcgcgatc atcgtcgtcg cagtggcagt ggatcgctgg cgctcgcgcg cataggagga
     1441 tctataacgg tgaacctcgg aaagccgctg ttcactttaa tctgtgttca tccgtgtctg
     1501 taaggcttag gcgacgagtg ctgttggctc ttcgctctcc cggttacagc tcgcgatgaa
     1561 ctgctcgagt tgtcgttcgc cgagcggagt cgagaacagg aatccctgac cgttctcgca
     1621 ttccaacttg cgcaacaagc gcaactgatc ttcagtctca acgccttcag caacaacgtc
     1681 cataccgagg ttctgcgcca acgcgacaat cgtgcgaacg atcgcgaggt tttcctcatt
     1741 ctccatcatc tgcgtcacaa acgaacggtc gatcttcagt gtgtcgatcg gaaaacgctg
     1801 gagatagctc aagctcgaat agccggtgcc gaagtcgtcg atgctcaatt gcaccccgat
     1861 gtttcgcagt tgcgtcaaca gcttgactgc cgcctcgatg ttgtcgctaa acactgattc
     1921 ggtgatctcc agcttgagac aacgtggcgg cagattcgat tcttcgagca actgtgcaat
     1981 gttctcaacc aggcccggct cgttaaactg cttcaccgac aagttgacac tgacgaatag
     2041 cggcggcgac gtcggataag tctcctgcca ctcgcgcgcc tgtcgacacg ctgaacgcaa
     2101 cacggtctgt ccgatgggaa gaatcaggcc agtctcttca gccactggaa tgaattcttt
     2161 cggcggtacc agtccaaact ccggatggtg ccagcgtacc agcgcttcga aaccgatcaa
     2221 catgtga
//
LOCUS       DASTEY010000692         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_296454, whole genome shotgun sequence.
ACCESSION   DASTEY010000692 DASTEY010000000
VERSION     DASTEY010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296454"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..999)
                     /locus_tag="VFO72_12030"
     CDS             complement(16..999)
                     /locus_tag="VFO72_12030"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9480071.1"
                     /translation="MRISVAMCTYNGAELLPAQLSSIAEQTRPPDEIIICDDASTDRT
                     RALLESFARTSPIPVSLSFAHQNAGSLKNFERAIGLCTGDVIALSDQDDVWRSDKLQR
                     IEETFRLSPTTGLVFSDADIVDKNLKPLGQRIWNEVGFDTHKQKLMRSRRSLDVLITG
                     WTVTGATMAFRSEYVKLALPIPANIAMIHDGWIALTIAAVADVAFIEEPLIKYRQHER
                     QQIGVPKRPVRQSERGLQAFESALRRRNSAADLHGILSVLEDRLIAQQNSFDCGRALA
                     FVGNYSLHLDVRANLPRKRLSRVPRIVRELLTMRYHEYANGFKSAAKDLVS"
     gene            complement(1236..>2184)
                     /locus_tag="VFO72_12035"
     CDS             complement(1236..>2184)
                     /locus_tag="VFO72_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9480072.1"
                     /translation="QRSLQLWQEFFTSDNSPTPLFHRTGVLWLARERDPYCEATLTNL
                     QAVGANCERLTQNELRARFPQLNFDGVDWAILELDTGVLMARRTVQAVTMKARAGGVD
                     YLEEGIPAPVTSRDLRTTSGREIVANQLVFACGPWLPNLFPELLGELIHVTRQEVFFL
                     GVPAGDDLFKPPRLPTWIDFTDLVYGMPALDGRGAKIAIDAHGPQFDPDHGQRIVSAE
                     STKAIREYLTRRVPLLAGAPVTETRVCQYENTSNGDFLIDRHPSLENVWLVGGGSGHG
                     FKHGPAVGEYVTAMITGSEEAEPRFSLATKEKIQERKVY"
BASE COUNT          441 a          609 c          652 g          482 t
ORIGIN      
        1 tcgagagatc agtactcaag agaccaggtc cttagcggcg ctcttaaaac catttgcata
       61 ctcgtgataa cgcatcgtca gcagttcgcg cactattctg ggcacgcggc tgagacgctt
      121 gcgcggcaag ttggcgcgga catcgaggtg aagagagtaa ttcccgacaa atgcgagcgc
      181 gcggccgcag tcgaacgaat tctgctgtgc gatcaggcgg tcttccaaca ctgagagtat
      241 tccgtgcaaa tcggcggctg aattacgccg gcgcaaggct gattcgaaag cttgtaagcc
      301 acgttcggat tgcctgactg gacgcttcgg cacgccgatc tgctgtcgct cgtgctggcg
      361 atatttgatt aacggttctt caataaaagc gacgtccgcg accgccgcaa ttgtcagcgc
      421 gatccagccg tcgtgaatca tggcgatgtt tgccggtata ggcaatgcca gtttcacata
      481 ctcggaacga aaagccatcg tcgcgccggt taccgtccag ccggttatca acacatcgag
      541 tgatcttcgg ctgcgcatca gcttttgttt gtgagtgtca aacccgactt cgttccagat
      601 tcgctggccc agcggcttca ggtttttgtc gacgatgtca gcgtcagaga agacgagtcc
      661 cgtagtcgga ctaaggcgaa acgtttcctc gatgcgttgc aacttatcgc tgcgccagac
      721 gtcgtcctga tcactcaaag cgatcacatc gccggtgcac aggccaatgg cgcgctcgaa
      781 gtttttcagc gagccggcgt tctggtgggc gaaagagagt gatacgggta tcggagacgt
      841 tcttgcgaac gactccagca acgcgcgcgt gcggtcggtg gaagcatcgt cgcagatgat
      901 gatttcgtca ggcgggcgtg tctgttcggc gatgcttgac aactgcgcgg gcagaagttc
      961 ggcgccgttg taggtgcaca tcgccacaga gattcgcata cgcggcattc tacatctccg
     1021 cctaaatcgt ggctaataaa gcttacagat gtagcttgaa gcgtctcgtg gtagctaggc
     1081 gtctcgtggt agcttggggc gtctcgtatc ctgggaccgc tggcgtctcg cctgcatggt
     1141 aacgcatcgg gcgctgccgt gactgtgcag gcgagacgcc tgcggtccca ggttaaaggc
     1201 gcgtccatgg gctactagac ctttcgttcc tgggcttaat agacctttcg ttcctggatc
     1261 ttttctttcg ttgccagact gaatcgaggt tcggcctctt cgctgccggt gatcattgcc
     1321 gtcacatatt cgccgacagc cggaccatgt ttgaaaccgt gaccggagcc gccgccgacc
     1381 agccaaacgt tttcgagaga cggatggcga tcgatcagaa agtcgccgtt ggaagtgttc
     1441 tcgtactggc agacgcgcgt ctcggttacg ggcgcacccg cgagcaaagg aacccgacgc
     1501 gtcaggtact cgcgaattgc ctttgtcgat tcggcagaca cgattcgctg gccgtggtcc
     1561 ggatcgaact gtggcccgtg cgcgtcgatc gcgatcttgg cgccgcggcc gtcgagcgcc
     1621 ggcattccgt aaacgagatc ggtgaaatcg atccatgtag ggagccgagg aggcttgaag
     1681 agatcatcgc cggcaggcac gccgaggaag aacacttcct gccgcgtaac gtgaatcagt
     1741 tcgcccagca attccggaaa aagattggga agccatggac cacacgcaaa tacgagttga
     1801 ttggcaacga tctcccttcc ggacgttgtt cgcaggtccc ggctcgtaac cggagccggt
     1861 atcccctcct ccaggtaatc cacgccgccc gctcgcgctt tcatcgtcac ggcctggacc
     1921 gtacggcgcg ccatcagtac gccggtatcc aattcgagga tcgcccaatc cacgccgtca
     1981 aagtttagct gcggaaatcg cgcgcggagt tcgttttgcg tgagtcgttc acagtttgcg
     2041 ccgaccgctt gaaggttagt gagcgttgct tcgcaatatg gatcgcgttc acgcgcgagc
     2101 caaaggacac cagttcggtg aaagagcggc gttgggctgt tgtcgctggt gaagaactcc
     2161 tgccagagtt gcagtgagcg ttgg
//
LOCUS       DASTEY010000693         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1383733, whole genome shotgun sequence.
ACCESSION   DASTEY010000693 DASTEY010000000
VERSION     DASTEY010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383733"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFO72_12040"
     CDS             complement(<1..894)
                     /locus_tag="VFO72_12040"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9480073.1"
                     /translation="MSTGSSVSSSSIRFRGLDAWRGFSALLIVLYHFTDQHKQAPADN
                     LLQYPIRLVQFGISQSYITVFLFFVISGFCIHMQWARARASGEEPRIRFGAFWKRRIR
                     RLYPPYVVVLALYLGLTAWTTGLDVTRFVVYDVAMHLLMLHNFDAHTAYSINGIFWTL
                     AIEEQLYLAYFLLLFMRVRWGWTITLTICLLARVGWMIFSHVVWLKTGFGLPVPEAAA
                     SHWFTWALGALAVEAMYGLIKLPNWSRDLRVAGVLVTAASVISAFLPTIHKDALLHEV
                     GWFLIHPLWGLGFFVLINWMVV"
     gene            complement(930..2360)
                     /locus_tag="VFO72_12045"
     CDS             complement(930..2360)
                     /locus_tag="VFO72_12045"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9480074.1"
                     /translation="MGPQATATSREEKKVENKEVRILVVDDEPTMADSLRQNFIEEGY
                     SVDIAPTGAAAIELFDQGGHHLAICDLQLPDMDGLEVLRHMKDARPTTEVIVVTGYGS
                     VQKAVEATKAGAFFFVEKPFDFEELQLLVEKALERRELVAETANMRRQLSTRAEYFNI
                     IGASKPMQTIYETIESVAKSDANVLIVGESGTGKELIANAIHYQSLRSKKPFIKVNCA
                     ALPKELIESELFGHTKGAFTGAHADKEGLVQHAAGGSLMLDEIAEMPVELQPKLLRVL
                     QERSYRKIGSEKTYAVDFRLVCSTNRPPADAIRDGLLRDDLFYRISTITIHVPPLRER
                     SEDIQLLTEHFLHMYAQKYERSISGVSQAAYQRLFSHSWPGNVRELQNVIERAVLLAK
                     DSQIEPIDLPFDNGTVPEGTSANVQWDVPPNMTLEEIERLVIERTLQRTGGNKQAAAN
                     LLGIYRPRLYSKIRKYNIDVPSLVSA"
     gene            complement(2402..>2930)
                     /locus_tag="VFO72_12050"
     CDS             complement(2402..>2930)
                     /locus_tag="VFO72_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480075.1"
                     /translation="EKGERPEDEQETIVKLIAGLDRAASDLATIVEYGRRIELKKQPG
                     FDVQKVMRTVCSGLDGPGAEIAVDSSSDSVQGSFDPLKLTDAFKWISGAAIKNRRHDD
                     EEPRPVSVKIVAVDDREVMIEWSGLRKIDHDPFHSFVGSEEIRLALAAKIIEAHGGSA
                     KLAEDRLVVQLPLSE"
BASE COUNT          686 a          851 c          749 g          644 t
ORIGIN      
        1 aaccaccatc cagtttatca ggacgaaaaa gccgagtccc cacaagggat gaattaaaaa
       61 ccagcccact tcgtgcaaca gagcatcctt atgaatcgtt ggcaggaaag cggagataac
      121 cgaagccgcc gtgactagca caccagcgac ccgcagatcg cgcgaccagt ttggcagttt
      181 tatcaggccg tacatcgctt cgacagcgag ggctccgagc gcccaggtaa accagtgtga
      241 ggcagccgcc tcaggcaccg gaaggccgaa tccagtcttg agccatacga cgtgactaaa
      301 aatcatccag cccacgcgcg cgaggaggca gatggtgagt gtgatcgtcc aaccccaacg
      361 aacacgcata aacaacaaca gaaaataggc gaggtacagt tgttcttcga tcgcgagtgt
      421 ccaaaaaatg ccgttgattg aatacgccgt gtgcgcatcg aaattgtgaa gcatcaatag
      481 atgcatggcg acgtcataaa cgacgaagcg cgtcacgtcc agacctgtgg tccaggccgt
      541 aagcccgagg tagagcgcca gaacaacgac atacggcggg tacagacggc ggatgcgacg
      601 cttccagaac gcgccaaacc gaatccgcgg ttcctctccg gacgcccgcg cgcgggccca
      661 ttgcatatga atacaaaaac cggaaattac gaagaagaga aagacagtga tgtagctctg
      721 agaaattcca aactggacca gccgaatggg atactgaagc aggttatcgg ccggcgcctg
      781 cttgtgttgg tcagtaaagt gatagagaac aataagtaac gcagagaacc cacgccacgc
      841 gtcgagtccg cgaaatcgta tgctgctgga agacactgat gagccggtag acattcgcga
      901 tgaccaaaaa cagcgattat cgcttcctgt tacgctgaca ccagtgacgg tacgtcgata
      961 ttgtattttc taatcttgct gtatagccgg ggccggtaga tgcccagcaa gttcgccgcc
     1021 gcctgcttgt ttccacccgt acgctggagc gtgcgctcga tcaccagccg ctcgatctct
     1081 tccagcgtca tgttcggtgg cacgtcccat tgaacgttcg cggacgtgcc ttccggcacc
     1141 gttccattgt caaacggtaa atcgatcggt tcgatctgac tgtctttcgc caacaacaca
     1201 gcccgctcga tcacgttctg cagctcgcgc acgttaccgg gccacgagtg actaaacaat
     1261 cgttgatacg cagcctgcga gacaccactg atcgagcgtt cgtatttctg cgcatacatg
     1321 tggaggaaat gctccgtcaa aagttgaatg tcttcactgc gttcgcgcag cggcggcacg
     1381 tgaatcgtga tcgtcgaaat gcgatagaag aggtcatcgc gcaatagtcc atcgcgaatc
     1441 gcgtctgcgg gaggacggtt cgtcgaacag acgagacgga agtcaacggc ataggtcttt
     1501 tccgaaccga tcttgcggta actcctttcc tgcaaaacac gcaggagttt cggctgtaac
     1561 tcgaccggca tttcggcgat ttcgtcgagc atcagcgacc cgccggcggc atgttggacc
     1621 aggccttctt tgtcagcgtg ggcacccgtg aaagcgcctt tcgtgtgacc aaacagctct
     1681 gactcgatca gttctttcgg cagcgccgcg cagttcacct tgatgaaagg cttcttcgag
     1741 cgcagtgact gatagtgaat cgcgttggca atcagctcct tgcccgttcc cgactcaccg
     1801 acaatcagca cattggcgtc ggactttgcc actgactcga tcgtttcgta gatcgtttgc
     1861 atcggtttcg acgcgccgat gatgttgaag tattcagctc gagttgaaag ctggcgccgc
     1921 atgtttgccg tctcggcgac gagttcgcga cgttccagcg ccttttccac cagcagttgc
     1981 aactcttcaa agtcgaaagg tttttcgacg aaaaagaaag cgccggcctt cgtggcttcg
     2041 acagcctttt ggaccgaacc gtatccggtg acgacgatca cttcagtggt aggccgcgcg
     2101 tctttcatat ggcgcagcac ttcgaggccg tccatatccg gcaactgcaa atcgcagatc
     2161 gccagatgat gtccaccttg atcgaagagt tcgatggccg ccgctccagt aggagcgatg
     2221 tcgaccgagt acccttcttc aataaaattc tggcgcagcg aatcggccat tgtcggctcg
     2281 tcgtctacaa caagaattcg cacctctttg ttttccacct ttttctcctc cctggaagtc
     2341 gccgtggctt gagggcccat cagttttact ccttcacaaa gaactacttg ttgtcgggga
     2401 gttattccga aagcggaagc tgcaccacaa gacgatcttc cgccagtttt gccgacccgc
     2461 catgggcctc gataatcttt gccgccaacg ccaatctgat ctcttcactg ccgacaaacg
     2521 agtgaaacgg atcgtgatcg attttgcgta atccggacca ctcgatcatt acttcccggt
     2581 catcaaccgc aactattttg accgacaccg gtctcggttc ttcgtcgtcg tggcggcggt
     2641 ttttgatcgc cgcgcccgat atccacttga atgcgtccgt aagtttcagc ggatcgaaac
     2701 tgccctgaac tgaatcggaa ctcgaatcga cggcgatttc agctcctgga ccgtccaaac
     2761 ccgaacagac cgtgcgcatt accttctgca cgtcaaaccc cggctgcttt ttcaactcaa
     2821 tccgccgccc gtattcaaca atagtggcca aatcactcgc cgctcggtcc aacccagcga
     2881 ttaacttcac gatcgtttcc tgttcgtcct caggacgttc gcccttctcg
//
LOCUS       DASTEY010000694         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_246521, whole genome shotgun sequence.
ACCESSION   DASTEY010000694 DASTEY010000000
VERSION     DASTEY010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246521"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1270
                     /locus_tag="VFO72_12055"
     CDS             134..1270
                     /locus_tag="VFO72_12055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9480076.1"
                     /translation="MSERLTQKPCDVVVIGGGIVGAACAAELAAEKLSVAIIEARLIG
                     GGATAAGMGHVVVMDDSEAQFVLTRYSQILWNELRDELPHEVEHDVCGTIWVAADDEE
                     MDEVFRKHRFYSERGVAVEVLDAQALAEAEPNLRPGLLGGLRVPGDSVIYPPCAAQFF
                     VERAVARGARLILGKAVDALTREGVRLRDGSTISAGLVVNAAGSWSPELTPELSVKKR
                     KGHLLITDRYPHFVRHQLVELGYLKSAHSVSTDSVAFNIQPRKTGQLLIGSSRQYQVE
                     DTRIDSSILTRMLQRAIGYVPALGKLSTLRAWTGFRAATPDKLPLIGPYNERLYLATG
                     HEGLGITTSLGTAKLLVDQIMNRQPDIALTPYLPSRPAQERAHA"
     gene            1263..1511
                     /locus_tag="VFO72_12060"
     CDS             1263..1511
                     /locus_tag="VFO72_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9480077.1"
                     /translation="MRELVTLNINGVSVSLPAGSMVAAAVLESGVTSFRRSVAGEARG
                     PLCGMGICFECRVRIDGVAHVRSCQTICENGMDVRTDE"
     gene            1504..2817
                     /locus_tag="VFO72_12065"
     CDS             1504..2817
                     /locus_tag="VFO72_12065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HET9480078.1"
                     /translation="MSSAPVDVVIAGAGPAGLAAAYHTATAGLRVVVVDDNPSSGGQI
                     WRGEQRKPTSNEAREWFERIRRAKIEFVHGARVFQQTNSNTLLAETAQGTVHLSYTNL
                     IIGTGARERFLPFPGWTLPNVMGAGGLQALVKTGLSISGKRVVVAGSGPLLLAVADYL
                     REQRAKVLLIAEQASASRLARFGLTLLGAGKSTQALELKRRLSGVKYLNGCWPIAAHG
                     AEKITSVTLFRAGKRWQVDCDYLACGFHLVPNTELAELLGCKIENGAVSVDRFQQTSV
                     PHVYAGGEATGIGGLDLSLIEGEIAGLAVANKHNEARKLFPAREKQRRFARVLNETFA
                     LRDELRDLATAATIVCRCEDIRLERLKTHNSWRSAKLQTRCGMGPCQGRVCGPAVGFL
                     FGWKVESVRPPVFPVRVESLATDRHRGHGDANDLARRDAGNHHGF"
     gene            2768..>3058
                     /locus_tag="VFO72_12070"
     CDS             2768..>3058
                     /locus_tag="VFO72_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET9480079.1"
                     /translation="MRMIWRGVMPATTTAFDEDLNIDHAFVAKHAAWLVDDGCTAIIA
                     PGSLGESATLTFDEKIALWSTLVSAVGDRVPVAAAIASLRTAEAVALAKRAAE"
BASE COUNT          617 a          786 c          996 g          659 t
ORIGIN      
        1 cgcgagcgga tggcgtgcga acctcatttc attgtcaccc ggcttcagcc gggtgcactg
       61 gaagctgctg ttctgaaacc gttttaacgg tttcttctaa tcggctcgaa cgacggccgg
      121 ctcgagttcg tgaatgagcg aacgcttgac gcaaaaaccc tgtgacgtgg tcgtgattgg
      181 cggcgggatt gttggcgccg cgtgcgccgc ggagttggct gctgagaaac tcagtgtggc
      241 gattatcgaa gcgcgcctga ttggcggcgg cgcgaccgcg gccggcatgg gccacgtcgt
      301 ggtgatggac gactcggaag cgcagtttgt tttgacgcgt tactcgcaaa tactttggaa
      361 cgaactgcgc gatgagttac cgcacgaggt cgaacacgat gtgtgcggca cgatctgggt
      421 cgcggctgac gacgaagaga tggacgaggt gtttcggaag caccggtttt attccgaacg
      481 cggcgtagcg gttgaagtgt tggacgccca agcgttggct gaagctgagc cgaacctgcg
      541 tcccgggctg ctgggtggat tgcgtgtgcc gggcgacagt gtgatctatc cgccgtgtgc
      601 tgcgcagttc ttcgtggagc gcgcggttgc gcgaggtgcg cggctgatct tgggaaaggc
      661 tgttgacgcg ttgacacgcg aaggcgtgcg attgcgcgat gggtcgacga tttccgcggg
      721 tttggttgtc aatgcggcgg gtagctggtc gccggagctg acgcctgagc tcagcgtaaa
      781 gaagcgcaag ggccatctcc taatcaccga tcgctatccg cattttgtgc gacatcagct
      841 tgtcgaacta ggatatttaa aaagcgcgca ttcggtgtcg actgactcgg tagcgttcaa
      901 tattcaaccg agaaagacgg gacaactgct gattggatcg tcgcggcaat atcaggtcga
      961 agacacgcga atcgactcgt cgatcctaac gcgaatgttg caacgtgcaa ttggatacgt
     1021 cccggcgctc gggaagcttt caacgttgcg cgcgtggacc ggatttcgtg cagcgacgcc
     1081 tgacaaactg ccgctgattg gcccatacaa cgaacggctc tacctcgcta caggtcacga
     1141 aggactcggc atcaccactt cactcggcac ggccaagttg ctcgtcgatc agatcatgaa
     1201 tcgccaaccc gacatcgcgt tgactcctta tctgccatcg cggccggcgc aggagagagc
     1261 tcatgcgtga gctggtcacg cttaacatca acggtgtctc agtcagcctg cctgcgggca
     1321 gcatggttgc cgctgccgtg ttggagtcag gcgtaacttc gtttcggcgc tcggtagcag
     1381 gcgaagcgcg cggcccgctg tgtggcatgg gaatctgttt tgagtgtcgc gtcaggattg
     1441 acggtgtagc tcacgtacgc agttgccaaa cgatctgcga aaacggaatg gacgtgcgca
     1501 ctgatgagta gcgcgcctgt tgacgttgtg atcgccggcg cgggcccggc cggacttgcc
     1561 gctgcgtatc acacggcaac ggcgggtttg cgcgtcgttg tcgtcgacga caatccgagt
     1621 tcaggcggac agatctggcg cggcgaacaa cgtaaaccga cttcgaatga agcaagagag
     1681 tggttcgaaa ggattcgccg ggcgaagatc gagtttgtgc acggtgcaag agtgtttcag
     1741 caaacgaact caaacacgct cttggccgaa acggcgcaag ggaccgtcca cttaagctac
     1801 accaacctga tcatcggcac gggcgcgcgc gaacgctttt tgccattccc cggctggacg
     1861 ctgcccaacg tgatgggcgc cggcggccta caggcgctcg taaaaacggg tttatcaatc
     1921 tcggggaaac gagtcgtcgt tgccggcagt ggtccattgc tgcttgctgt tgcggattat
     1981 ctgcgcgaac aacgcgcgaa ggtgttgctg attgccgagc aggcgtcagc atcgcgtttg
     2041 gcaaggtttg gtctcactct gctcggcgca ggcaagtcaa ctcaggcttt ggaattgaag
     2101 cggcgtctga gcggagtgaa gtatctcaac ggctgttggc caatcgcggc gcacggcgct
     2161 gagaagatca cgagcgtgac tctgtttcgc gccggaaaac gttggcaggt cgattgtgat
     2221 tacctggcgt gcgggtttca tctcgtacca aataccgaat tggcggaact gcttggctgt
     2281 aagattgaga acggcgccgt gagtgtcgat aggtttcagc aaaccagcgt gccgcatgtc
     2341 tatgccggtg gcgaagcgac aggtatcggc ggactcgacc tgtcactgat tgaaggtgag
     2401 attgccgggc ttgcagttgc caataagcac aacgaagcgc ggaagttatt tcccgcacga
     2461 gaaaagcaga ggagatttgc gcgggttttg aatgagacgt tcgctttgcg tgacgagcta
     2521 agggatcttg ctacagccgc gacgatcgtg tgtcgctgtg aagacattag attggaaagg
     2581 ttgaagacac acaattcgtg gcgatcggcg aagttacaga cgcgttgcgg catgggacca
     2641 tgccagggtc gcgtgtgtgg tccagccgtg gggtttttgt ttggttggaa agtcgaatcc
     2701 gtacggccgc cggtttttcc ggtgcgagtc gaaagtttgg ccacagatag acacagagga
     2761 cacggagatg cgaatgatct ggcgcggcgt gatgccggca accaccacgg cttttgatga
     2821 agatctgaac atcgaccatg cgttcgtggc gaagcatgcc gcatggctcg ttgatgacgg
     2881 ctgcaccgcg atcatcgctc cgggttcact tggcgagagc gccacgttga cgttcgacga
     2941 aaaaatcgcg ctttggtcga cgttggtcag cgccgttggc gatcgggttc cggttgcggc
     3001 cgctattgct tcgttgagaa cggccgaagc cgtcgcactt gcaaaacgtg ctgccgaa
//
LOCUS       DASTEY010000695         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_411249, whole genome shotgun sequence.
ACCESSION   DASTEY010000695 DASTEY010000000
VERSION     DASTEY010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411249"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /locus_tag="VFO72_12075"
     CDS             complement(<1..1935)
                     /locus_tag="VFO72_12075"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HET9480080.1"
                     /translation="MKPTQELVKKDQRQEPAAAFDRVEPPQSYNYGVDPNADNEVHLL
                     DYWRAIRKRLWLVLGIVALVTMLSVVYVARKPDFYEAQARVQVDLEETGNLVNNSRPL
                     YGPTDDPIYFNTQLQILVSPGLMRRVVRTLDLEHNPDFFKGHPAQRQSTWQTLLRMVG
                     LSKKAPDTAAKPPDQLPLTNTVAQATAREDLNEAKRLAPYVSTILSGLKVEPVKETRG
                     YYKETRLIDIKFTHNVPDVTAKIVNAIAETYVFANLEKRTEANSTTGDFLQKRIAELQ
                     QQIRTSEERLVNYAKNNQIISLDPNQNTVVERLAGLNRQLLEAENDRKTAEAAYNAAK
                     NPDAASALADDGAKQTNEIEAKLVELRQKRAQLLVEATEEAPEVKEVDQQVTELDQQL
                     KDLRSRKSATLLTNLHTRYQQTLEREQSLRKAFEHQRAQTLSQNEAAINYRIIQQEIE
                     TNKSLLNGLLQGAKENDVVLAGKPNNISIVDYALTPDSPVGPNRTRTVIAAFFLSIGL
                     GLGLALFFEYLDDTVHSTEEVERVLHLPALAVIPSVGSATRRRVLPASSKSTALQKQN
                     GNGNGNSELLMNVDGRSPLAEAYRHLRTSVLLSTAGRAPKSLLVTSSLPGEGKTTTAV
                     NTAISLAQTGASVVIIDADMR"
     gene            complement(1952..>2168)
                     /locus_tag="VFO72_12080"
     CDS             complement(1952..>2168)
                     /locus_tag="VFO72_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480081.1"
                     /translation="KIRVLRQEPGTSVRKEITIDLYAIEKKRSEDLALAPNDIIDVPT
                     SAGKSFLRSLIQGVVPGVGQLPVRVVP"
BASE COUNT          375 a          570 c          686 g          537 t
ORIGIN      
        1 gcgcatgtcg gcatcgatga tgacgacgct cgcgccggtt tgcgcgagac tgatcgcggt
       61 gttgaccgcg gtcgtagtct tcccttcacc aggcaagctc gacgtgacga gcaaagactt
      121 cggcgcgcgt cccgcagtcg agagcaacac ggacgtgcgc aggtgtcgat aagcttcagc
      181 gagcggcgaa cgaccatcga cattcatcaa cagctccgaa ttgccgttgc cgtttccgtt
      241 ctgcttctgg agcgccgtcg attttgaaga ggcaggcagc acacgtcgtc gcgtggcgct
      301 gccgaccgaa ggaatcaccg cgagcgcggg caggtgcagc actcgttcca cctcttcggt
      361 cgaatgcacc gtgtcgtcga gatattcgaa aaacagcgcc aggccgagac ctaaaccgat
      421 ggaaagaaag aacgcggcaa ttaccgtgcg cgtgcggttt ggaccaacgg gactgtcggg
      481 cgtgagcgcg taatcgacga tcgagatgtt gtttggcttg cctgccaaca cgacgtcgtt
      541 ttctttcgcg ccctggagca gtccgttcag caatgacttg ttggtttcga tctcctgttg
      601 aatgatccgg tagttgattg ccgcttcgtt ctgcgaaaga gtctgcgcgc gctggtgctc
      661 gaaagctttg cgcaacgact gttcgcgttc gagtgtttgt tgataacggg tgtgcaggtt
      721 tgtcagcagg gtcgccgatt tccggctgcg caaatctttt aactgctgat cgagctcggt
      781 tacctgctgg tccacctctt tgacttcggg cgcttcttca gttgcctcga ccagcagttg
      841 agcgcgcttt tgccgcagct cgacgagctt ggcttcgatc tcgttggttt gtttggcgcc
      901 gtcgtcagcc agtgcgctgg cggcgtcggg atttttggcg gcgttgtaag cggcttcagc
      961 agtcttgcga tcgttctctg cttcgagcag ttgccggttg agacctgcta aacgctcgac
     1021 gaccgtgttc tgattgggat cgagcgagat gatctgattg ttcttggcgt agttgacgag
     1081 gcgttcttca gacgtgcgaa tctgttgctg aagttcggcg atacgcttct ggagaaagtc
     1141 gccggtggtg ctgttggctt cagtgcgctt ctcaagattc gcgaagacgt aagtctcagc
     1201 gatggcgttt acgatctttg cggtcacgtc cggaacgttg tgcgtgaact tgatatcgat
     1261 caggcgtgtt tccttgtagt aaccacgcgt ttctttgacg ggctcgactt tgagcccgct
     1321 gaggatcgtg ctgacgtagg gcgcgagacg tttcgcttcg ttgagatcct cgcgagcggt
     1381 ggcctgcgcg acggtgtttg taagcggcag ttgatccgga ggtttcgcgg ccgtgtcagg
     1441 tgccttcttg ctcagtccga ccatccgcag cagtgtttgc caggttgatt gtctttgcgc
     1501 cggatggcct ttgaagaagt ccgggttgtg ttcgagatcg agggtgcgga cgacgcgtcg
     1561 catcagtccg ggactaacga ggatttgtag ttgcgtgttg aagtaaatcg gatcgtcggt
     1621 tggaccgtag aggggtcggg aattgttgac gagattgccg gtctcttcaa ggtcgacctg
     1681 gacgcgcgcc tgcgcttcgt agaagtccgg tttgcgcgcg acgtagacca cggacagcat
     1741 cgtgacgagc gcgacgatgc ccaacacgag ccagagacgc ttgcggatcg cgcgccagta
     1801 gtcgagcagg tgtacttcat tgtcggcgtt gggatcgacg ccgtagttat acgactgcgg
     1861 cggctcgact cgatcgaaag cggcggccgg ctcttgccgc tgatctttct tgacaagctc
     1921 ttgtgtgggt ttcatctggg tcttccgttg tttagggaac aactctgacg ggcaactgtc
     1981 cgactccggg cacgacgccc tgaatcaggc tgcgcaggaa actcttaccg gcggaggtcg
     2041 gcacgtcgat gatgtcgttc ggcgcgagcg cgagatcctc ggaacgcttc ttctcaatgg
     2101 cgtacaagtc aatcgtgatc tctttgcgca cggacgtgcc cggctcttgt cgcaacacgc
     2161 gaatcttg
//
LOCUS       DASTEY010000696         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1515698, whole genome shotgun sequence.
ACCESSION   DASTEY010000696 DASTEY010000000
VERSION     DASTEY010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515698"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFO72_12085"
     CDS             complement(<1..935)
                     /locus_tag="VFO72_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480082.1"
                     /translation="MTVAFLFLAASSTAAQSIDPNAPSPVRSNTVTGRIAARDLGDAR
                     VTDHYYAFTGTPGDLLITVQSNNLNGDVDVFTAGTLRPLLKLTLYAEIGTPVTKGIYL
                     RKREDLVLRVEARSPNDDEGTYRLRFGGSFEPIVGGPDIAEAETPATEPTRAPGTRRV
                     TSTGARIKEPEPPVTEVAAAPTPEPTPEPTPEAKPVETPAATPERSPEVETERPAPTR
                     NTRGRRPAGRRPAPRAPDTAASETPAPKPPPVEPEPEVGPRLVIETNDGTLINRSMST
                     VRRVTVENGQVVVTGRDGKIDRILLSNIVRMSISP"
     gene            1020..>2144
                     /locus_tag="VFO72_12090"
     CDS             1020..>2144
                     /locus_tag="VFO72_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9480083.1"
                     /translation="MKLSCTLLLFLLSSLPAFAQSWTAKLDKDIRFYQPTEMGVVIAG
                     TEKSLYAIDGSSGEVLWRRKDTSLDETDVAPVPGTDLLLLSFEKGDKARIEAVDVLTG
                     NSIWRSEKIKGAVMQISVDPATNLLAVVLVKDAKNRARDGFKRRPLLHVLDLASGDEL
                     WKYEASEVEMMPSRWPEDSGKEVAYTLDNYYPPVFIDNRFYVFYEGVTSFEARSGNER
                     LREKYRVNEDGLALTEAEPIFTEGYIYVSAHGRVRAISRQTGDTEWEAKDLGLTPEMI
                     LAGDILYVRTGGQFTRIKDGETIERGPYGVSAIDINSGKVLWRYKGADKGITNLLLPN
                     RNTIAMADEDDLIFVDTGNGKRTTRVSHKIEQPSFGLLNDR"
BASE COUNT          430 a          581 c          646 g          487 t
ORIGIN      
        1 taaggcgaaa tagacatgcg cacgatgttg gataagagaa tgcgatcgat cttcccatcc
       61 ctcccggtca cgaccacctg accattctca accgtcacac gtctcacggt gctcattgag
      121 cgattgatga gcgtgccgtc attcgtctcg atcacgagtc gtggtccaac ttcaggctcg
      181 ggctcgaccg gtggtggctt cggtgccggc gtttcgctgg cggcagtgtc aggtgcgcgt
      241 ggcgccggac ggcgtccggc gggtctgcgt ccacgagtgt ttcgagttgg cgcgggtcgt
      301 tctgtttcaa cttcgggaga tctttccggt gtggctgccg gtgtctcgac cggtttcgct
      361 tcgggcgtcg gttcgggtgt tggttcaggc gtcggcgctg ccgccacttc agtcacgggc
      421 ggctctggtt ctttaatcct tgctcctgtt gacgtgacac gtcttgttcc tggcgcccgg
      481 gtaggttccg ttgcaggcgt ttctgcttcc gcgatatcag gcccaccaac gatgggctca
      541 aacgagccac caaagcggag acgatatgtt ccctcatcgt cattcgggct gcgcgcttcc
      601 acacgcaaga ccagatcttc gcgcttgcgc aggtagattc cttttgtaac tggagttccg
      661 atttcagcgt agagcgtgag cttgagtagc ggtcgcaacg tgccggcggt aaacacgtcg
      721 acatcgccgt tcaggttgtt gctctgcaca gtgatcagca gatcgccagg cgtgccggtg
      781 aaggcgtagt aatgatccgt tacccttgca tcgcccagat cgcgcgcggc gatccggccg
      841 gtgacagtgt tgctgcgcac tggagacgga gcgttcggat cgatcgactg agcggcagtt
      901 gagcttgcgg ccaaaaacag gaacgcaacg gtcaagaacc ctgtgccttt gcgcctttgc
      961 gtgaaaaact ttgccatgtt gcgccgcaaa gcatatcaga tttaggttat gctagtcgca
     1021 tgaagttgag ttgtaccctg ctgctttttc tgttgtcttc ccttcccgct ttcgcccaat
     1081 cgtggacggc aaaactcgat aaagacatcc gcttttatca accaacggag atgggcgtgg
     1141 tgatcgcggg cacggagaag tcgctgtatg cgatcgacgg ctcgagcggc gaagtgctct
     1201 ggcgtcgcaa agacacttcc cttgacgaaa ccgatgtcgc gcccgttccc ggcactgacc
     1261 tgttgttgtt gagttttgaa aagggcgaca aggcgcggat cgaagccgtg gatgtgttga
     1321 ctggcaactc gatctggcgc agcgaaaaga tcaagggcgc cgtgatgcag atttcggtcg
     1381 atccggcgac gaatctgctc gctgtcgtgc tggtgaaaga cgcgaagaac cgcgcgcgcg
     1441 acggtttcaa acgtcgcccg ctgctgcacg ttctggatct cgcttccggt gatgaactct
     1501 ggaaatacga agccagcgaa gtcgagatga tgccgtcgcg ctggccggaa gattcgggca
     1561 aagaggtcgc ttacacgctc gataattact acccacccgt attcatcgat aatcgctttt
     1621 atgttttcta tgagggcgtt acgtcgttcg aagcgcgtag cggcaacgag cgtttgcgag
     1681 aaaagtatcg cgtcaacgaa gacgggctcg cgctgactga agcagaaccg atcttcacgg
     1741 aagggtatat ctatgtgtcc gctcatggac gtgtgcgcgc catctcgcgt cagaccggcg
     1801 acaccgagtg ggaagcgaaa gatctcgggc tcacgccgga aatgattctc gccggcgaca
     1861 ttctttacgt gcgcaccggc ggccagttca cgcgaatcaa agatggcgag acgatcgagc
     1921 gtggtccgta tggcgtgagc gcgatcgata tcaactcggg caaagttttg tggcgttaca
     1981 aaggcgcgga caaagggatc accaaccttc tactccccaa tcgaaacacg atcgcgatgg
     2041 cagatgagga tgatctgatc ttcgtggata caggcaacgg caagcgaacg actcgcgtgt
     2101 cacacaagat cgagcaacct tcgtttggat tgctgaatga tcgc
//
LOCUS       DASTEY010000697         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1004983, whole genome shotgun sequence.
ACCESSION   DASTEY010000697 DASTEY010000000
VERSION     DASTEY010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004983"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1012)
                     /locus_tag="VFO72_12095"
     CDS             complement(158..1012)
                     /locus_tag="VFO72_12095"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9480084.1"
                     /translation="MRFRLTALLMVFAFTTMVQAQSALQEMVKTEQAFSKMAEEKNTH
                     DAFMAFIADDGLLFRPGAVNGKKWMIEHPPPPPKNTDKKPLLAWQPKFAGMSASGDMG
                     FTTGPWESKADIKDEKPQGYGHFVTVWKKQTDGSWKFVVDLGISHPQSGGPQTLWQPP
                     DTNKTPSFKPVNVPAATEELLTRDRKFSFADYASPDVRLYRAGNLPYIGKQAAVEALA
                     KTKGQIAWQPIGGDVSRAGDLGYTHGTYEIADDTKNVIERGSYVRIWQKQGGEWRVVM
                     DVTNVHPK"
     gene            complement(1026..2534)
                     /locus_tag="VFO72_12100"
     CDS             complement(1026..2534)
                     /locus_tag="VFO72_12100"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9480085.1"
                     /translation="MMLALSLKGIHKSFGPTVALDGVDLELRQGEVHALIGENGAGKS
                     TLMNIIAGSLQPDQGKMENHGKPYSPSNPLDARTNGIALIHQELSLCPHLSVAENVLM
                     GIESSRFGWLDRDQLIARTNEVLKTFHHADIRPEKRVGDLSVAARQIVEISRALAAQA
                     RIILMDEPTSSLQREDVSQLFTLIRKLSAEGISVIYISHFLEEVRQISDRFTVLRDGR
                     SVATGDIANTSDDELIAKMVGRAQQTLFPVRTPPTEKPETILEVRNLASPPLLKDASF
                     DLRRGEILGIAGLMGSGRTPLLRAIFGLEPPESGSIAVNKKSLPSGRSTPAMRLIEGL
                     GYLSEDRKGDGLTLNQSIADNVTVTKFDSCSRWGWLDLSAQRKQAERLVNALKIRTQG
                     VQQDVGSLSGGNQQKVIVARLLHQDADVFLLDEPTRGIDIGSKAQVYETIAACAANNK
                     AVLMVSSYLPELFGMCDRLAVMSRGRLSEVRNIDQWTPESVLQAAIGAADLR"
BASE COUNT          522 a          725 c          706 g          612 t
ORIGIN      
        1 attctccgag aagatttgca gtttgtgatt ttcgagtggc gagactgtgg cccaggtgaa
       61 gaagtcgagg gcgaccggca ttacgaagcc gtcgttgtag tcggtgtgtt caccgataag
      121 attgacccgc cccggtgctc gaaagatgcg cgtttcgcta ttttgggtgg acattcgtca
      181 catccatcac aacccgccac tcgccgcctt gcttctgcca gattcgaacg tagctgccac
      241 gttcgatcac gttcttcgtg tcatctgcga tttcgtaagt gccatgtgtg taaccaagat
      301 cgcccgcgcg cgacacgtcg ccgccgatcg gctgccacgc gatctggcct ttggtctttg
      361 ccaatgcctc gacggcagcc tgtttgccga tgtacggaag gttgccggct cgatagagtc
      421 gtacgtcagg cgaggcgtaa tcggcaaaac taaattttcg atcgcgagta agcagttctt
      481 cagttgcggc gggaacatta acgggcttaa aggatggggt cttattcgtg tccggtggtt
      541 gccacaacgt ttgtggtcca cctgactgcg gatgactgat accgagatcg acaacgaact
      601 tccacgagcc atccgtctgt ttcttccaga cggttacaaa atgaccatac ccctgcggct
      661 tctcgtcctt gatatcggct ttggattccc atggaccagt cgtgaatccc atatcgcccg
      721 atgcggacat gcccgcaaac ttcggctgcc atgcgagcaa cggcttcttg tccgtattct
      781 ttggcggagg cggcggatgc tcgatcatcc acttcttgcc attcacggct ccaggtcgaa
      841 acaacaatcc atcatcggca atgaacgcca taaacgcgtc gtgcgtgttc ttctcctccg
      901 ccatcttcga aaacgcctgc tcagtcttca ccatctcctg caacgccgac tgcgcctgta
      961 ccattgtcgt aaaagcaaaa accattaaaa gtgcggtcaa tctaaatctc ataatccgtc
     1021 cttgatcagc gtaaatccgc ggcccctatt gctgcttgta acacgctctc cggcgtccat
     1081 tgatcaatat tccgaacttc cgacagtctc ccacgactca tcaccgccag tcgatcgcac
     1141 atcccaaaca gttcaggcaa atacgagctc accatcaata ccgctttatt gtttgctgcg
     1201 catgccgcta ttgtttcgta aacctgcgcc ttgctgccga tgtcgatgcc gcgcgtcggc
     1261 tcgtcgagca aaaacacgtc agcgtcctgg tgcagcagtc gcgcgacgat caccttctgt
     1321 tgattaccgc ctgacagaga cccaacatcc tgctgcacgc cctgcgttct aatcttcagt
     1381 gcgttgacaa gcctttcggc ctgtttgcgt tgcgcgctga gatccagcca gccccagcgc
     1441 gaacacgagt caaattttgt gaccgtaaca ttgtcggcga tcgactggtt caatgttaag
     1501 ccgtcgcctt tgcgatcttc gctgagataa ccgagaccct cgattagacg catcgcgggc
     1561 gtgctgcgtc cactcggcaa actcttttta ttcacggcga tgctaccgct ttccggaggt
     1621 tcgagaccaa agatcgcgcg cagcagcggc gtgcgaccgg agcccatcag cccggcgatg
     1681 ccgagaatct cgccgcgacg cagatcgaag ctggcgtctt ttagcagcgg cggagatgca
     1741 aggttgcgca cttcgagaat cgtttccggt ttttcggttg gtggcgtgcg gacggggaag
     1801 agcgtttgtt gcgcgcgccc gaccattttg gcgatcagtt cgtcgtcgct ggtgtttgcg
     1861 atgtcgccgg ttgcgacgct gcggccgtcg cgcagtacgg tgaagcgatc cgaaatctgg
     1921 cgcacttctt caagaaagtg gctgatgtag attacggaaa ttccttcggc gctcaacttc
     1981 cgaatcaatg tgaagagctg gctaacgtct tcgcgttgca ggctgctggt gggctcgtcc
     2041 atcaggatga tgcgcgcttg tgcggcgagt gcgcgggaga tctcgacgat ctgtcgtgca
     2101 gcgactgaga gatcgccgac gcgtttttcg ggccgaatgt ctgcgtgatg aaaagttttg
     2161 aggacctcgt tggtgcgggc gatgagttga tcgcgatcga gccaaccgaa gcgtgacgac
     2221 tcgatgccca tgagcacgtt ttccgcgacg ctgagatgcg gacagagaga aagctcctga
     2281 tggatcaggg cgatgccgtt ggtgcgcgcg tcgagtgggt tggagggaga ataaggttta
     2341 ccgtgattct ccatctttcc ctgatccggc tgtagtgatc cggcaatgat attcattagc
     2401 gtgctcttgc cggcgccgtt ctccccgatg agcgcgtgca cctcgccctg ccgcagctcg
     2461 agatcgacac catcgagcgc gacggttgga ccaaaggatt tgtgaatgcc ttttagagag
     2521 agcgctaaca tcattcaagc acagaagaaa gaacaagtca ggaag
//
LOCUS       DASTEY010000698         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1367444, whole genome shotgun sequence.
ACCESSION   DASTEY010000698 DASTEY010000000
VERSION     DASTEY010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367444"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2072
                     /locus_tag="VFO72_12105"
     CDS             <1..2072
                     /locus_tag="VFO72_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9480086.1"
                     /translation="PRALRTVSIEQFEAWARRGLSTSRADARARRSYFSLETRGSYEA
                     LHSGSAGLALDSVQHLLRLYVEALTGREVEVAPLAAVPDEARIGDGRTIHLPSLVNEF
                     GDEDLDFKLFKVLAAHAAGQIEFGTYERGGKLGVIRAAIAETYENADARDAFAIADQL
                     LQTTERQVNTDSTELNPDYRDVLKLFPLPQLARRIFGTLENGRVDRRLRQSYRGLRRD
                     LDLIREHLGRSRPRIDELPAVLVPFELLFQITMLGGAREDARQYYGQVVSELETIVSE
                     YLSDPQATVAETLIATIRVYDLFKSVVPTDDSVQQVEAPEDQTEAEDDSDLTERMKQR
                     QTQQMPQRRDARELFNAWNDPSPEGEPDELTGADAWSEAETPEHALVEGEVAYNYDEW
                     DRELTDHRIGWCRVIEKRVRHGDRSFVEHTRERHKGVISSIRHQFQLMKPENLTRVAN
                     EVDGEEFDLNAVIDYVIDRRADGHQSERIYTKRLRRRRDVAVSFLLDQSSSTARTIGR
                     HPLQPYTRPGRRIIEIEKEGLVLMSEALEAVGDVYSINGFTSEGRRNVKFYVVKDFNE
                     KYGDEVKQRIGGINYQNNTRLGAAIRHATMRLAKQEARTRLLIVLSDGRPYDHDYGDA
                     RYAREDTREALRQAKNEGIIPFCITIDRESEAELRDLYGEVGYTIIDDVLSLPERLPG
                     IYRRLTT"
BASE COUNT          479 a          594 c          635 g          419 t
ORIGIN      
        1 gtccgcgtgc gttgcgcacg gtgtcgatcg aacagtttga agcgtgggcg cgtcgcggac
       61 tttcgacgag ccgggcagat gcgcgtgcgc ggcggagtta tttctccctc gaaacgcgcg
      121 gcagttacga agcgttgcac agcggcagcg cgggcctggc attggattca gtgcaacacc
      181 tgttgcgctt gtacgttgaa gcgttgacgg gacgcgaggt tgaggtcgcg ccgctggccg
      241 ccgtgcccga tgaagcgcgg atcggtgacg ggcgaacgat ccatcttccg tcactcgtta
      301 acgagtttgg cgatgaggat ttggatttca aactgttcaa agttctcgcg gcgcatgctg
      361 ccggacagat cgagtttggc acttacgaac gcggcggtaa gttgggtgtg attcgtgccg
      421 ctatcgcaga gacttatgag aatgccgatg ctcgagatgc gtttgcgatc gctgaccagt
      481 tgctgcagac aaccgagaga caggtaaaca cggattccac ggagttaaat ccggattata
      541 gagatgtgct gaagctgttc ccgctgccgc aactagcgcg acgcatcttc gggacgctcg
      601 aaaacggccg tgtcgatcgc cgcctgcggc aaagctatcg cggtctgcgt cgcgatctcg
      661 atctaatccg cgaacatctc ggccgcagcc ggccgcgcat cgacgaactt cccgcggtgc
      721 tcgttccctt cgaactgctg tttcagatca caatgctcgg cggcgcccgc gaagatgcgc
      781 gccagtatta cggccaggtc gtctccgaat tggagactat agttagcgaa tacctgtctg
      841 atccgcaggc aactgtagcc gaaacactga tagccacgat ccgtgtttac gatctgttca
      901 agtccgtggt cccaaccgac gattcagtcc agcaagtcga ggcgcccgaa gatcaaaccg
      961 aagccgagga cgattccgat ctcaccgaac gtatgaaaca gcggcagact caacagatgc
     1021 cgcagcggcg cgatgcccgc gaactcttca atgcctggaa cgacccctca ccggaaggcg
     1081 aacccgacga actcacgggc gcggacgcat ggtcggaagc cgagacgccc gaacacgcgt
     1141 tggtcgaggg cgaagtcgcc tacaactatg acgagtggga tcgcgagttg accgatcatc
     1201 gcatcggctg gtgccgtgtg attgaaaaac gtgtgaggca cggcgaccga tctttcgtcg
     1261 aacacacacg agaacgacac aagggcgtga tctcttcgat ccggcaccag tttcagctga
     1321 tgaagccgga aaacctcaca cgcgtggcga atgaagtcga tggtgaagag tttgatctca
     1381 acgcggtgat cgactacgta atcgatcgcc gtgcagatgg acaccagtcg gagcgaatct
     1441 acacgaaacg gctgcggcgg cgacgcgacg tggcggtgtc gtttctgttg gaccagtcgt
     1501 catcaacagc gcggacgatt ggccgccatc cgttgcaacc gtacacgcgg cccggccgcc
     1561 ggatcatcga gattgaaaaa gaggggttgg tgttgatgag cgaagcgctt gaagccgttg
     1621 gggacgtcta ctcgattaac ggtttcacct cggaagggcg cagaaacgtg aagttctatg
     1681 tcgtaaaaga tttcaatgag aagtacggcg atgaagtgaa gcaacgaatc ggcggcatca
     1741 actaccagaa caacacgcgt ctcggcgcgg cgattcgtca cgcgactatg agacttgcca
     1801 agcaggaagc acgcacgcgt ctcttgatcg tgctctccga cggtcgtcct tacgatcacg
     1861 attacggcga tgctcggtac gcgcgtgagg acacgcgcga agctctgcga caagcgaaga
     1921 acgaaggtat catcccgttc tgcataacca tcgatcgcga atcagaagct gagctgaggg
     1981 atttgtatgg tgaagtcggc tacacgatca tcgacgacgt gctctcacta ccagagcggt
     2041 tgcccggtat ttaccggcgc ctcacaactt aactccgttt aatccgtggt ccccaacctc
     2101 caccaccagg cgtctcgatt ctaattc
//
LOCUS       DASTEY010000699         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_81954, whole genome shotgun sequence.
ACCESSION   DASTEY010000699 DASTEY010000000
VERSION     DASTEY010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81954"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..517
                     /locus_tag="VFO72_12110"
     CDS             311..517
                     /locus_tag="VFO72_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET9480087.1"
                     /translation="MSRITGKVKWFNNSKGYGFIEQPGSSDIFVHYSAIQGDGFKTLE
                     EGQEVEFEVTQGPKGPQAEKVTKM"
     gene            complement(998..1639)
                     /locus_tag="VFO72_12115"
     CDS             complement(998..1639)
                     /locus_tag="VFO72_12115"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HET9480088.1"
                     /translation="MGLRENKLLRAKLQAQSKNKYATGDNQPSADEQLTRLEDDIRRL
                     RIEFDIFFNGAAKRPPYETKGRVETLLKRLGDDRTLTYAQRYRYNSLAARYNAFRDLW
                     RRTMQGREEGRDPASAARANAKRETEIEMDPVSISCMDPHKEIEVVKSLYTSLIEAKQ
                     KCGEPTEGFSFPKFHRLVAQKADTLKEKTGCERVLFSVALDEGHVSFKAKADG"
     gene            1741..3153
                     /gene="selA"
                     /locus_tag="VFO72_12120"
     CDS             1741..3153
                     /gene="selA"
                     /locus_tag="VFO72_12120"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET9480089.1"
                     /translation="MTSNLRHVPSVDQLLRTDVARRLRESIGLKRVTAIARTVAAEIR
                     SSIRNNELDVAANGHSAEALLAEAARRMEQTAKREDETGIKKVINATGVLLHTNLGRA
                     PLSRAARTAIDEAAGYCNLEYDVATGTRGKRGARVESLLKDLTGAEDALVVNNCAAAA
                     LLILTVLAADGETIVSRGELVEIGGDFRVPDVMATSGTRMIEVGTTNRTHLEDYRRAI
                     NTQTRLIMRVHPSNYRIVGFASSPELSELAALAHEYNLPLYEDAGSGVLSDLTAYGVV
                     DEPTVGEIVAKGVDVISFSGDKLLGSAQAGLIVGKRSIVDRLRKHPLYRALRSDKLRL
                     AALEATLIAHRRENAAGDVPALQMLSLTAGEIELRARSVIEQVAPTELKLAIESGDSA
                     VGGGTAPTSNLRSVVITVSHLRKSASDLEQQLRRSSPSVIARISENRVLLDLRTVSPD
                     DFQAVVAALKNLDLTMAGIV"
BASE COUNT          665 a          877 c          876 g          737 t
ORIGIN      
        1 aaaactttct tgcttgacag gtttaagatc ttcgcttagt atgcgccgtc gttcaatgcg
       61 gggacggtag tccagagccc agcatcccga tccacaagct gtacgaaatg agtttcgagt
      121 tttgcccccg tagtctgtgc atgctgaaat ggcgctcccc gcgctgttca tcatgcattt
      181 tttgttggtt gtgtgtttcc gtctagcgcc atttcagggt gcgacccggc tcacaagcat
      241 taaccaagtt ggtgggaaag tcttgcggct ttcgctccgt tttaccgaat cgttaggagg
      301 attcgttgtt atgtctagaa taaccggtaa ggtcaagtgg tttaacaatt cgaagggcta
      361 tggttttatc gagcagcccg gctcttcgga catttttgtt cactatagcg ctatccaggg
      421 cgacggcttc aagaccctcg aagagggcca ggaagttgag tttgaggtca cccaagggcc
      481 aaagggacct caggctgaaa aagtgacgaa gatgtaatgt ctccccggct tgcctacgca
      541 aagccactga ctgcttccgg tcacgggtcc ggtaagctca caagaagaaa ggcgacctcc
      601 tgcggtcgcc ttttcttttg cccgggagcg caggcgtttc gcctgcatgc cgccggtgaa
      661 aacgctgttg gaacattgca ggcgagacgc ctgcggtccc aggtggcttt gcctcaacca
      721 ttggtagttc ggtctttgga cttacggcaa cggcgcctga cttgtagtct tctttcattc
      781 tcaccgggct tcagcccggt gttaccgcca cgcggttttg agaaaccgtt ttaacggttt
      841 cgctggcgaa tcaacggaaa ccgttgaaac ggtttcctcc gtcttcactg ctcctgcacc
      901 gggctaaagc ccggtgagaa tgagagagga cttttgaggc aaagcggtcc caggtaaaag
      961 tcgcctttag attaagatta acagtcgacc ctttgaatta accgtccgct tttgctttga
     1021 agctcacgtg cccctcgtcc agggcgaccg agaaaagcac ccgctcacat cctgtttttt
     1081 ctttgagagt gtcagccttc tgggccacga gccgatgaaa cttcggaaac gagaaacctt
     1141 ccgtcggctc accgcatttc tgctttgctt caattagcga ggtgtaaaga ctcttgacga
     1201 cttcgatctc cttgtgaggg tccatacagc taatcgaaac ggggtccatc tcgatctccg
     1261 tctctctttt ggcgttcgcg cgcgcggccg acgccggatc gcgtccctct tcacgtcctt
     1321 gcatggttcg gcgccagaga tctcgaaacg cgttgtagcg agcagcaagc gagttgtagc
     1381 ggtaacgctg cgcgtaggtt aacgtgcgat cgtcgccgag tcgcttcagc agtgtttcga
     1441 cgcgaccttt cgtttcgtaa ggcggacgct tcgcggcgcc attgaagaat atgtcaaact
     1501 cgattctgag ccggcgaatg tcgtcttcca aacgcgtcag ctgttcgtca gcactcggct
     1561 gattgtcgcc agtggcgtac ttattcttgc tttgtgcctg tagtttggcc ctaagaagct
     1621 tgttctcacg taatcccatt aaatgatctc ctggcgaggt gattagggcg gggttcgcgt
     1681 cgaatgcaac taacgcgagt gaatgcagtc tataatacgg ccatggacga aggcaagaaa
     1741 atgacttcaa atctccgcca tgtgccatcg gtggaccaac tgctgcgtac ggacgtggcg
     1801 cgccggttgc gggaatcgat cggtttgaag cgcgttactg ccatcgcccg gaccgtcgcc
     1861 gcagagattc gttcgtcaat tcgcaacaac gaactcgacg ttgcggccaa cggccactct
     1921 gctgaagcgt tgctcgcgga agccgcgcgt cgcatggaac aaactgcgaa acgtgaagac
     1981 gagactggta tcaagaaggt gatcaatgcc actggagtgt tgcttcacac gaacctcgga
     2041 cgcgcgccac tttcacgcgc cgcgcgcacg gccatcgatg aagctgcggg ttattgcaac
     2101 ctcgaatatg acgttgccac gggcacgcgc ggcaaacgcg gcgcgcgcgt cgagtcactt
     2161 ctgaaagatc ttaccggagc tgaagacgcg ctcgtcgtaa acaactgcgc cgcagcggca
     2221 ttgttgattt tgacggtcct ggccgcagat ggagaaacga tcgtttcgcg cggcgaactc
     2281 gtcgagatag gtggcgactt ccgcgtgccg gacgtgatgg cgacttccgg tacgcgcatg
     2341 attgaagtcg gcactaccaa ccgcacgcac ctggaagatt atcgacgcgc gatcaacaca
     2401 caaacgcgtc tcattatgcg cgttcatcct tcaaactatc gcatcgtcgg ttttgcgagc
     2461 tcaccggaac tgtccgaact ggcagcactg gcccacgaat acaaccttcc gctctacgag
     2521 gacgccggct cgggagtgtt gagcgacctg accgcgtacg gtgttgtcga tgaaccaact
     2581 gtcggtgaga ttgtcgcgaa gggtgtggat gtgatctcgt ttagtggcga caaactgctc
     2641 ggctccgcac aggctggatt gatcgtcggg aaacgatcga tcgtcgatcg cctgcgtaaa
     2701 catccactct atcgcgcgtt gcggagcgac aaacttcggc tggccgccct tgaagccaca
     2761 ctcatcgctc accggcgaga gaacgcggcc ggcgacgtgc ccgccctgca aatgctctcg
     2821 ctaactgctg gtgaaatcga actgcgcgca cgatccgtga tcgagcaggt ggcgccaacc
     2881 gaactaaaac tcgcgatcga atccggcgac tcagcggtcg gcggcggcac agcgccaacg
     2941 tcgaatctgc gctcagttgt gatcacggtt tctcatttgc gtaaatctgc ctcggacctg
     3001 gagcagcaac tgcgcaggtc ttcaccctcg gtaatagcga gaatctcgga aaacagggtc
     3061 ctgctagatc ttcgcacagt ctctcctgac gattttcagg ccgtcgttgc cgcgctaaaa
     3121 aacctcgatt taacaatggc tggcattgta taatc
//
LOCUS       DASTEY010000700         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_691568, whole genome shotgun sequence.
ACCESSION   DASTEY010000700 DASTEY010000000
VERSION     DASTEY010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691568"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2546
                     /locus_tag="VFO72_12125"
     CDS             <1..2546
                     /locus_tag="VFO72_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9480090.1"
                     /translation="RETGSSHVIATEFVEGETLRSRLNRTALSVTEALDVAIQVADAL
                     AAAHKAGIIHRDIKPENIMLRPDGYIKVLDFGLAKLSEQASPTVAAEAPTIQVRTGSG
                     IVIGTAGYMSPEQARGLGVDSRSDIFSLGAVIYEMLARRKPFEGDTPSDTLAAILKTE
                     PPPLSRVIAGVPAELTRIVTKCLKKDREERYQVVKDLWLDLKALRQELEFQKNRAGEA
                     PDVLRPSAVAGEPTALFAEPRPTVARSGISTITESLSVEIKRHKFGAAVALTLIALVL
                     AASGFGIYKLLNRETAVAHFSEINLAQLTNSGNAIDAAISPDGKYIVYVLSDRSAQSL
                     WIRQVSTANDKLIVPPAPVGYFGMTFSPDGNDLYYVIKANLDAGTLYRIPVLGGTPVK
                     ITEKLDGPVSFSPDGKQFVFIRGNYPTAAGSALIIANIDGAAERELSVKNFPERFSPI
                     FFAGPSWSPDGKLVAASVATTGGQSRLLGFSVADGSEQQLWQQPFPFTGRVQWLPDMT
                     GLLVVAGESPAAAQLWVVNHPEGAGRRVTNDLSTYRSIGLTQDGKTFTTVQAQGLVNL
                     WVAPDGDVARASRLPTGNVSFYSSSGNNVSWTPDGRIVFVSNEGGNPNIWITKPDGSE
                     RKQLTANDAANFSPVVSPDGKYIVFGLWRGGKKTLWRMDVDGRNPVQLTFGVGDSFPS
                     ISPDSRWVIYTAPSEARPTLWKVSIDGGTPAQVINRNATMGVVSPDGKFIAYTYPESA
                     DALAPPNRLALIPFEGGQPIKTFEVRTSGTVLAVIQWAPDGKSVLYTVTANNFTNVWS
                     QPIDGGPAKQVTQFKDMLMTGFAWSHDGKQLACTRGNLVRDAVLIRDLK"
     gene            2543..>2787
                     /locus_tag="VFO72_12130"
     CDS             2543..>2787
                     /locus_tag="VFO72_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480091.1"
                     /translation="MSHRLIPILFVLLTTASVCAQELRQYNIKPSDLPPPNPAEDAVN
                     PPRVIPRPAGAQLILPPGFSANTFAEGDFTQPRWLALA"
BASE COUNT          630 a          809 c          774 g          574 t
ORIGIN      
        1 tccgcgagac cggctcgtcc cacgtcatcg cgacggaatt cgtcgaaggt gaaacactgc
       61 gcagtcgcct gaatcgaacg gctcttagcg tcactgaagc tctcgacgtc gcgattcagg
      121 ttgccgacgc gctcgcggct gcgcacaagg caggaatcat tcatcgcgac atcaaacctg
      181 aaaacatcat gctgcgcccg gatgggtaca taaaggtgct cgacttcggg ctcgcaaaac
      241 ttagcgaaca agcatcaccg actgtagcgg ccgaagcgcc gacgattcag gtgcgcaccg
      301 gatccggaat cgttatcggc accgcgggtt acatgtcgcc cgaacaggcg cgcggactcg
      361 gcgtcgatag tcgatccgac atcttcagtc tcggcgctgt catttacgag atgctggcgc
      421 gtcgcaaacc attcgaaggc gacacaccaa gcgacacgct ggccgcgatt ctcaaaaccg
      481 agccgccgcc actttcacgt gtgatagcgg gagttcctgc cgaactaaca cgcatcgtta
      541 ccaagtgtct caagaaagat cgggaagaac gttatcaggt agtgaaagac ctctggctcg
      601 acctgaaagc actgaggcag gagttggagt ttcagaagaa cagagcaggc gaagctcccg
      661 acgttctgcg gccgagcgcc gtggcgggtg aaccaacggc gctctttgcc gaaccacgac
      721 ccacagtcgc gcgcagcggc atcagcacga ttaccgagtc gctcagtgtc gagatcaaac
      781 gtcacaagtt cggcgccgcg gtcgcattga cgctgatcgc actcgttctc gccgccagcg
      841 gatttggtat ttacaagctg ctgaaccgcg aaaccgctgt cgcgcacttc tcagagatca
      901 atcttgcgca gcttaccaac tcaggtaacg caatcgacgc cgccatctcg cccgacggca
      961 agtacatcgt gtacgtcttg agcgaccgca gtgcgcagag cctctggatt cgtcaggtaa
     1021 gcacggcaaa cgacaagctg atcgttccgc ccgctccggt cggatacttt ggtatgactt
     1081 tttcgcccga cggcaacgat ctttactacg tgatcaaagc aaaccttgac gcgggtacgc
     1141 tctatcgaat tccggtgctc ggcggcacac ccgtcaagat cactgagaag cttgatggac
     1201 cagttagttt ctcgcctgac ggaaagcagt tcgtgtttat ccgcggcaac tatccaaccg
     1261 ccgccggcag tgcgctgatc attgccaaca tcgatggcgc cgccgaacga gaactctcgg
     1321 tgaaaaactt tcccgagcgg ttttcgccga tcttctttgc tggtccatcc tggtctcccg
     1381 acggaaagct cgtcgcagct tcggtcgcta ccacgggtgg acaaagtcga ttgctgggtt
     1441 tctcagttgc tgatggcagt gaacagcagt tgtggcaaca accatttcct ttcaccggac
     1501 gtgttcaatg gctgcccgac atgaccggct tgttggtcgt cgccggagaa agccccgcgg
     1561 ccgcgcaact ttgggttgtc aatcacccgg aaggcgccgg gcgtcgcgtc acaaacgatc
     1621 tcagtactta ccgctcgatc ggattgacgc aggatggaaa gacattcacc acagttcaag
     1681 cacaaggatt ggtgaacctg tgggtcgcgc ccgacggcga cgtggccagg gcttcacgac
     1741 taccaaccgg aaacgtcagt ttctattctt cgtctggaaa caacgtgtca tggacgccgg
     1801 acggaagaat cgtgtttgtt tcgaacgaag gtggcaatcc caacatctgg atcacgaaac
     1861 ctgacggcag cgagcgcaaa caactcacgg caaacgatgc ggcgaacttt tcgcctgtcg
     1921 tgtcgccaga cggcaagtac atagtttttg gtctctggcg cggcggcaaa aagactctgt
     1981 ggcgaatgga tgtcgacggc cgcaatccag tgcagttgac gttcggcgtg ggagattcgt
     2041 ttccgagtat ttcgcccgat agtcgttggg taatctacac cgccccgagc gaagcaaggc
     2101 ccaccctttg gaaagtttcg atcgatggtg ggacgccggc tcaagtcatc aatcgcaatg
     2161 caaccatggg tgtcgtttct cctgatggca agtttatcgc ttatacgtat cctgaatctg
     2221 cggatgcatt ggcgccaccg aaccgattgg cgttgatacc attcgagggc ggccagccaa
     2281 taaagacgtt tgaagtgcgg accagtggta cagtattggc cgttattcag tgggcgccgg
     2341 atggtaaatc agtgctttat accgttacgg caaacaactt cacgaacgtt tggagtcagc
     2401 caatcgacgg tggtccagcg aaacaggtca cgcagttcaa agacatgctg atgactggct
     2461 ttgcgtggtc acacgacgga aaacaactcg cgtgcacgcg cggcaacctg gttcgcgacg
     2521 ccgtactgat tcgagatctc aaatgagcca cagattaata ccgatccttt tcgttttact
     2581 gacgaccgca tccgtgtgcg cacaggagct gcggcaatac aacatcaaac cctcggatct
     2641 gccaccaccg aatccggcag aagacgcggt gaacccgcca cgagtcatac cgcgtccagc
     2701 cggtgcgcag ttgatcctgc cgcccggttt ttctgcgaac acatttgccg aaggtgattt
     2761 cacgcagccg cgctggctgg cgctcgc
//
LOCUS       DASTEY010000701         5416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1021404, whole genome shotgun sequence.
ACCESSION   DASTEY010000701 DASTEY010000000
VERSION     DASTEY010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021404"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFO72_12135"
     CDS             <1..334
                     /locus_tag="VFO72_12135"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480092.1"
                     /translation="PPMHEVVFTDKRQSRKGVHTLDIAKRLIDYGFHPMTIYFPLIVQ
                     GAMLIEPTESVGHQELDQFIAAMKSIAREAVENPELVLNAPHDTRIGRLDEAAAARKP
                     VLRWRTPD"
     gene            complement(354..773)
                     /locus_tag="VFO72_12140"
     CDS             complement(354..773)
                     /locus_tag="VFO72_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9480093.1"
                     /translation="MNLVDVDFIDDRDRLVELAPVAAASIQHHVFVCTGKSCSAADSA
                     EVKNAFEHELKTRGLQYGTIKKGRNPNGTVVLTECGSVGFCAVGAAVMIYPEGVWYAQ
                     VRPADVPEIVEEHIVNGRPVERLALLRVARKPADQTD"
     gene            940..2553
                     /locus_tag="VFO72_12145"
     CDS             940..2553
                     /locus_tag="VFO72_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9480094.1"
                     /translation="MSTGLVIHISSGDDRHTEILSDDSVRIGASESCDLRLRSSSLPK
                     SANNSVLLELSRANGSYRVTDFNSAVPLTLNGKPVNPGSEIRDGDEFRIADSEIVLHF
                     YPVRALPAVVPAAARGTHVAPFIEAAAIESAATARRDDAKVFLREFTRELVREINPST
                     KLITLGIALALVGGMLYLGFAMYKELQRSRRLIDDQRVQLGQMRDQVSKTNEQIINID
                     ESNKQIQYSLSLAVKLRSQYGSGVCLIAGSYYYVESGTGRPMRYPEAQTNDSGAAVQS
                     GTAPEALTPEGNAAIAEYEFVGTGFHIGQGYVLTNRHIAQPWIADDRAQSIAGSLNAQ
                     PRLRKLIAYFPNITQAIPLKFKGAAQRDDLAVCSLDISEIPETIPILPLEADSDAVAV
                     GKTVVMMGYPSGPDRLLALLDDAESRGIQQRYGTPMAQLEYLAQTKRIQPLTTQGSIT
                     DLDVRRIVYDARTAEGGSGAPLFGPSGRVIGVNFAVFTENQASNFAVPIRFGLPLLQR
                     AGWAAPAKEAKEGEATSQSSGHASQSGSR"
     gene            2897..4921
                     /locus_tag="VFO72_12150"
     CDS             2897..4921
                     /locus_tag="VFO72_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480095.1"
                     /translation="MTIQTRRHWHLKASLIFVFLLVISPLTPTITRAKTLESEENDPA
                     SALSQILRLERIEVPGGAELITVHAKLGGLESTGNDNWVPLVSILRDTLGDNIAENNR
                     LRYVWPLTYTRPSTKQKLAAAIPFFYARVGNKEKLSKVPPAALDLASPESQVWDKIFW
                     TALQGILLDPYGTPIKASTSSYRRNTSDYRRSHIIRALSVLALYEAVKGESAFTRAEM
                     SNIQARLLLTEKTFGGLVDDSNLQSYYAKQTTQTRDERGHNWELLRQRAESESLYFEP
                     LLMPDGSATHALLWVAKRDLIKQQGSRYSSRFLNIANPWTDKRLLSWKGYVETRYFDS
                     DNHPVDSQTPGAEAVEMIPLGLYGLDNPKIPMLLVDFRDSYNPKKREMSRRVLNDVTR
                     NVLSLSKFGNLPFFLGRTVFDFVTGRRGMDINQPSRLATYSQLKLLLALDTSMDPELR
                     SEVSGRLEKASLNPFENDLKAEAHIATEQYKALVAFAVDPDGLAAKIERDRRAEMTSQ
                     EHGAKAQLAFRVLNVLSFGKYVHREELTDDMEDRLDIARRLDYHTKFLQQITRSSADV
                     DVTWNLDDVRRSVRFIANHGSEAGGNAAAATAKLFARTKDDDIRRACIDGLSRIHSRK
                     AKTELLRISQNNNVDKALRDLASEYLRGNTPRVQPIAATVSGTPVEAGQP"
     gene            4924..>5416
                     /locus_tag="VFO72_12155"
     CDS             4924..>5416
                     /locus_tag="VFO72_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480096.1"
                     /translation="MDGKTTKLSLRLCAFAGILLFLLCSTSLAQTKRLVIIKCDGLPY
                     DVVDRVVKQRNPQSGKSLLPWIDHIFYQRGARLSNFYVRGMSLSAPSWSLLETGQHLQ
                     IKGNVEFDRFTLHTYDYLNFFPLYLAGVNGTRVDMRAVEVLDSLGLPMLPDAYPHNER
                     YITY"
BASE COUNT         1287 a         1553 c         1413 g         1163 t
ORIGIN      
        1 cccgccgatg cacgaagtcg tcttcaccga taaacgacag agtcgcaaag gcgttcacac
       61 gctcgatatc gcgaaacgat tgatcgacta cggtttccac ccgatgacga tctatttccc
      121 gctaatcgtg cagggcgcaa tgctgatcga accgacggaa tcggtgggtc atcaggaact
      181 cgaccagttc attgcggcga tgaaatcgat tgcgcgtgag gcggtggaga atccggaact
      241 ggttttgaat gcacctcacg acacgcgcat cggtcgtctc gacgaggcag cagccgcacg
      301 caaacccgtg ttgcgatgga ggacacctga ttaatgctga taagaatccg gatttaatct
      361 gtctgatccg cgggttttct tgccacccgc aacagcgcta accgctcaac cgggcggccg
      421 ttaacaatgt gctcctcaac tatctcaggt acgtcagcag gtcgcacctg cgcgtaccaa
      481 acaccctcgg gataaatcat cacggctgca cccacagcac aaaacccaac cgatccgcac
      541 tcagtcagca cgaccgtccc gttcggattg cgacccttct ttatcgttcc gtactgtagc
      601 ccgcgcgtct tcaactcgtg ctcaaaagca tttttaacct cagcactgtc ggcagccgag
      661 cacgactttc ccgtacacac aaacacatga tgctgaatcg aagccgccgc aacgggcgcc
      721 aactcaacta accgatcgcg gtcgtcgatg aaatccacgt caaccaagtt caattcctca
      781 cttcgacttc atgctaccac tacgcatgca attacgttaa ctgtttggct ccacttcatg
      841 tagaatgtgc ttcgagggcg gccggagata accgcgcatg agctgttagg cggtcggatc
      901 cgggcgctcg ctttgtgaat gatggttttg taacttttta tgtcaaccgg cctggttatc
      961 cacatctcct cgggcgacga ccggcatacc gaaatcctct ccgacgatag cgttcgcatt
     1021 ggtgcttccg aaagctgtga cctgcgactc cgctcgtcca gtttgccgaa aagcgccaac
     1081 aacagcgttt tgctcgagtt aagccgggcg aacggctcgt atcgtgtcac cgatttcaac
     1141 tcagcagtgc cgctcacgct taatggaaaa cccgtcaatc cgggcagcga aatccgtgac
     1201 ggcgacgagt ttcgcattgc agattcagaa atagtactgc acttctatcc cgtgcgagcg
     1261 cttccggccg tcgtgcctgc ggcagcacga ggaactcacg tcgccccgtt tatcgaagcc
     1321 gctgcaatcg agtcggctgc caccgccagg cgcgacgatg ccaaggtgtt cctccgcgaa
     1381 ttcactcgcg agctggtgcg cgaaatcaac ccgagcacga aactgataac tctcggaatc
     1441 gcgctcgctc tcgtaggcgg catgctctat ctcgggttcg cgatgtacaa agagctccag
     1501 cgcagtcgca ggctcatcga cgaccaacgc gtgcagctcg gccagatgcg cgatcaggtt
     1561 tcaaaaacta acgagcagat catcaacatc gacgagtcga acaaacagat ccagtacagt
     1621 ctttctctgg cggtaaaact gcgcagccag tacggcagcg gcgtttgcct gatcgcaggt
     1681 tcatactact acgtcgagtc aggcacaggt cgtccgatgc gataccccga agcgcagacg
     1741 aacgacagtg gcgccgctgt tcagagtggc actgcgcctg aagcattgac gcctgaaggt
     1801 aacgccgcaa ttgcagagta tgagttcgtc ggcaccggtt ttcacatcgg acaaggctac
     1861 gtgttaacga accggcacat tgctcagcca tggatcgctg acgatcgcgc gcaaagtatt
     1921 gccggcagtc tcaacgctca gccgcgtctg cgaaaattga ttgcttactt tccaaacata
     1981 acccaggcga tcccactaaa gttcaaaggc gccgcacaaa gagacgacct cgccgtttgt
     2041 agtttggaca tatcggagat tccggaaacg attccgattc ttccgttgga agcggattcc
     2101 gatgcagtcg cggttggcaa gacggtggtg atgatgggtt atccgtcagg acctgatcgg
     2161 ttgttggcgc tgttggacga cgctgagtcg cgaggaattc aacaacgcta cggcacgccg
     2221 atggcccaac tcgaatatct cgcccaaaca aaacgcattc aacctctgac aactcagggc
     2281 agcattaccg atctcgacgt gcgaagaatc gtatacgacg cacgcacagc cgaaggcgga
     2341 tcgggcgcac cactctttgg accgtccggt cgtgtgatcg gagtcaactt tgcagtcttc
     2401 acagaaaacc aggcgtcgaa ctttgccgtt ccgattcgct ttggtttgcc tctactccaa
     2461 cgcgccgggt gggcggcgcc ggcgaaagaa gctaaagaag gggaagctac cagtcagtcc
     2521 tcaggccatg catctcaatc cggatcgagg tgatcctcag ctacctgtca gaattgccag
     2581 tttattcgtt aacaccgctt gaaacacaat cgtcctaata catgctccgc cggttcggtt
     2641 cggcgacaac gaggcaatac atcgaggcat cagatctcga actgtggcaa tgcgccacgt
     2701 gtcacaggtc tgacacaaag tcggtccata aggacttaga gtaaggtata tttatctcgt
     2761 gcccgtgcgg gcatagctct tgcagattga aaaccgaacc tgagggtttt tgagcatctt
     2821 gtacgaattt gtaatctgag gatgcagtag aaactcccca tctgccagat tttgtttagt
     2881 gttcaaacga agaaggatga cgatccaaac gcgtaggcac tggcacctga aggcgagctt
     2941 aattttcgtt tttctgctcg ttatctcgcc attaactccc acaatcacca gggcgaaaac
     3001 cctcgaatcc gaggaaaatg acccagcgtc ggcgctctct cagatccttc gtctggagcg
     3061 catcgaagtt ccgggtggcg ctgaactgat cactgttcac gcgaaactcg gaggactcga
     3121 gtcaactgga aacgacaact gggttccact ggtgtctatt ctgcgcgaca ctctcggcga
     3181 caatatcgct gaaaataatc gactgcgata tgtttggccg ctgacataca cacggccctc
     3241 aaccaagcag aaactagcag ccgcaattcc gttcttctac gcgcgcgtcg ggaataaaga
     3301 aaaactaagc aaggtgccgc cggcagcact tgacctcgcc tcgcccgaga gccaggtatg
     3361 ggacaagatc ttctggacgg cgttgcaagg cattttgctt gatccttacg gtacgccgat
     3421 caaagcatcg accagttctt atcgacgcaa caccagtgac tatcgccgat cgcacatcat
     3481 ccgtgcgctt tcagtactgg cgctgtatga agcagtgaag ggcgaatcag ctttcacccg
     3541 agcggagatg tcgaacatcc aggcgcggct gctgctgact gagaagacgt ttggcgggct
     3601 ggttgacgac tccaacctgc aaagctatta cgcgaagcag actacacaga cacgtgacga
     3661 acgcgggcac aattgggagt tgctgcgtca aagagctgaa tctgaatcgc tctacttcga
     3721 acctctattg atgccggacg gcagcgccac acacgcgctg ctctgggtcg cgaaacgcga
     3781 tctgatcaaa caacaaggct cgcgttacag cagccgtttt ctcaacatcg cgaatccatg
     3841 gactgataag cgactgctga gttggaaggg ctacgttgaa actcgctact tcgactccga
     3901 caatcatcca gtcgacagtc aaacaccagg cgccgaagcc gtagagatga ttccgcttgg
     3961 tctctacggg ctcgacaatc cgaagatccc gatgctgctc gtcgatttcc gtgacagcta
     4021 taatcccaag aaacgcgaga tgtcgcgccg cgttttgaac gacgtgacgc gcaatgtcct
     4081 atcgctatcg aagttcggca acttgccatt ctttctcggc cgcaccgtgt tcgattttgt
     4141 gactggccgc cgcgggatgg acataaatca gccatcgcgt ctcgccacgt attcgcaact
     4201 gaaactcttg cttgcactgg acacatcgat ggatccggag cttcgttcgg aagtgagcgg
     4261 acgactcgag aaggcctcgc tgaacccgtt tgaaaacgat ctaaaggcgg aagcgcatat
     4321 cgccacggag caatataagg ccctcgtagc ttttgcggtg gacccggatg gactggctgc
     4381 gaagatcgaa cgtgaccgcc gcgccgaaat gacttcacag gagcatggag ccaaggcaca
     4441 gctagctttc cgtgtgttga atgtgctgag ttttggaaag tacgttcatc gcgaagagct
     4501 gaccgacgac atggaggatc gactcgatat tgcgcgtcgt ctcgactatc acaccaagtt
     4561 cctgcagcaa atcacgaggt cgagtgccga tgttgatgta acgtggaacc tcgacgacgt
     4621 cagacgatcg gtgcgcttca ttgccaatca cggatcagag gcgggtggga atgcggccgc
     4681 cgccaccgcg aaactctttg cacgcacgaa agacgacgat attcgtcgtg cctgtatcga
     4741 cggcctgtcg cgaattcaca gccgcaaggc gaagaccgag ttgctgcgaa tctctcaaaa
     4801 taacaatgtc gacaaagcct tgagagatct cgcgagcgag tatctgcgcg gtaatacccc
     4861 cagagtccag ccaatagccg caaccgtcag cggtacgcca gttgaagcgg gtcagccctg
     4921 acgatggacg gcaaaacaac caagttatct ttgcggcttt gcgcctttgc gggaatactt
     4981 cttttcctgc tttgcagcac ttcgcttgcg cagacgaaac gcctcgtcat tatcaaatgc
     5041 gacggactgc cttatgacgt tgtagaccgc gtggtgaagc aacgtaatcc gcagtcaggc
     5101 aagagcctgc ttccctggat cgaccatatc ttctatcaac gtggcgcgag gctctcgaac
     5161 ttctacgtac gtggcatgag tttgtcagcg ccttcctggt cgctgctcga aacgggtcag
     5221 cacctgcaga tcaaaggcaa cgtcgagttt gatcgtttca cgctgcatac ttacgactat
     5281 ctgaacttct ttccgctcta tctcgccgga gtcaacggca cacgcgtgga tatgcgcgcc
     5341 gtggaagtgc tcgactcgct cggcctgccg atgctccccg acgcctatcc gcacaatgag
     5401 cgttacataa cgtact
//
LOCUS       DASTEY010000702         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_741376, whole genome shotgun sequence.
ACCESSION   DASTEY010000702 DASTEY010000000
VERSION     DASTEY010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741376"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VFO72_12160"
     CDS             complement(<1..1376)
                     /locus_tag="VFO72_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9480097.1"
                     /translation="MRKDALRLMIVDDEEAARYGMRRALTTLGYNITEAGSAEAARAL
                     LKQHEPDLLLLDVNLPGMSGLDFLREIKNANGNAPLVIIVTAHGSERMAVEAVKAGAH
                     DYLSKPFEVDDLRLVVKNAAETLQLRRENYSLRRRIEVERSQRGALIGNSQSMQRVRS
                     MIEKVAETDATVLVRGESGTGKELVARELHERNSVRRNGSFVAVNCAALPSELIESEL
                     FGHEKGAFTGAAARREGKFEQADGGTLFLDEIGDMSANVQAKLLRALEERRIERLGSN
                     ESIPVDVRIVSATHRPLEQEITNGQFRADLFYRLRVLTIDIPPLRERREDIPLLADTF
                     VRLAAQRYDLPERALSQGTLKRLLEYNWPGNVRELKNTIDRAVIMAEGDEIAPRDLPD
                     EITAGLPVSATGEGGDETDGLRVPFTADFREDRREFERRYITRCLEHTHGNVTRAAEI
                     LDMHRQSL"
     gene            complement(1485..2114)
                     /locus_tag="VFO72_12165"
     CDS             complement(1485..2114)
                     /locus_tag="VFO72_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480098.1"
                     /translation="MKLRNLLTFILVGMCLFVGGLSVQAQSNQADTKEDETNVDTQLY
                     LILGTNQDVVDSKLPSSLDGVVKQLRATLPFKNYRLAATLINRSRNEGKLELSWVGAP
                     LTSTPSAAQLMTRSSFRVRHIKLVRGSEGQPMVQMTGFVFNAQIPILANAAAVAAANP
                     EPPVFSYEGTTLSTDISMREGEPVIVGTLNAGPSGDAVILVVSVKRTAK"
     gene            complement(2189..3805)
                     /locus_tag="VFO72_12170"
     CDS             complement(2189..3805)
                     /locus_tag="VFO72_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9480099.1"
                     /translation="MNLLIPIVILVVVLGFLIALMLLSRNYVKVSPNQAAVISGRSRK
                     LPDGTVVGYRLVRGGATLIVPFLEKVEYLNLNVLTVPLATSRAYTVQGVPVSVKAVAN
                     VKIKGDDDSLRAAAERFLGMKPEEFHRLVFQTLEGHLRAILGTLTVEEINNDRQSFAQ
                     KLTTEAAGDLEKMGIGLDALTIQEISDEEGYLDALGKRRTAEVQRDAEIGQAEALRDS
                     KIKAALAMQEGERVRLASEAEIAASTRATEVKRAQYQAEIKREQATAEQAGPLSEAKA
                     RQEVVAEEVRVEKIRTQEQISVQEQEVLRREKELEATVVKKAEAERRARVLLAQGQQE
                     SAILEAEGQKQALIASAEAESQKLQREGAGRASAIEAEGRAEAEKIRALGLAEAAKIE
                     AQGLAQAKAVEAQGLAEAAAIKEKAAAWREFNDAARLQTILERLPGIIEATAPALKAI
                     SEPLGNIEKVVMIDHGGDGNGGGSGGLNRFAQTGPTMIYALLQQLQALGLNVPEILKQ
                     LGTTNGDEVVKVATEIKTKEKEPVANKNSD"
     gene            complement(3830..>4013)
                     /locus_tag="VFO72_12175"
     CDS             complement(3830..>4013)
                     /locus_tag="VFO72_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480100.1"
                     /translation="VSIAPGSLGQVSCRVGEERIEKLARARDNREIKAGSTVRVDEVA
                     GDSLIVSPYDSTYTQN"
BASE COUNT          771 a         1185 c         1084 g          973 t
ORIGIN      
        1 tgtagcgact gtcggtgcat gtcgagaatc tcggcggcgc gggtcacatt gccgtgggtg
       61 tgttcaagac aacgcgtgat gtaacgccgc tcaaactccc gccgatcctc gcgaaagtcg
      121 gccgtgaacg gcacgcgcaa accgtccgtt tcgtcgccgc cttcgccggt cgcgctcacc
      181 ggaaggccgg ccgtgatctc gtccggcaga tctcgcggcg caatctcgtc gccttctgcc
      241 atgatcaccg cgcgatcgat tgtgttcttc agttcgcgaa catttcccgg ccagttgtac
      301 tcgagcaaac gcttcaatgt tccctggctg agtgcccgtt ccggcagatc gtaacgctgg
      361 gctgcgagcc gcacaaatgt gtctgcgagc aacggaatgt cttcgcgccg ttcgcgcaaa
      421 ggtggaatgt cgatcgttag gacgcgcagg cgatagaaga gatcggcgcg aaactgtccg
      481 ttggtaatct cctgttcgag tgggcgatga gtcgcactca cgatacgcac gtcgacagga
      541 atcgattcgt tagacccgag acgttcgatt cgccgttctt cgagtgcgcg tagcagtttg
      601 gcttggacgt tggcgctcat gtcgccgatt tcgtcgagaa acagagtgcc accatcggcc
      661 tgctcaaact tcccttcccg gcgtgctgcc gcgcccgtaa atgcgccctt ttcgtggcca
      721 aacagttccg actcgatcag ctctgaaggc agcgcggcgc agttgaccgc tacaaacgaa
      781 ccattgcgcc gcacactatt tctctcatga agttctcgcg caacgagttc ctttcccgtt
      841 ccggattcgc cgcgaaccag gacagtcgcg tcagtttccg cgactttctc aatcatgctg
      901 cgcacgcgtt gcatcgactg tgagttaccg atgagtgcgc cgcgctgtga acgctcgact
      961 tcaatgcgcc ggcgcaagga gtagttttcg cgccggagtt gaagtgtttc ggcggcgttc
     1021 tttacgacca gccggagatc gtcgacttca aaaggcttcg acagatagtc atgcgcgccg
     1081 gccttcacgg cttcaacggc cattcgttcg gaaccgtgtg cggtgacgat aattacgagc
     1141 ggggcatttc cgtttgcatt tttgatctcg cgcaggaaat cgagaccgct catgccggga
     1201 aggtttacgt cgagtagcag gaggtcgggt tcgtgttgtt tgaggagggc gcgggcggct
     1261 tcggcggaac ctgcttcggt gatgttgtag ccaagcgtag tcagggcccg ccgcatgccg
     1321 taacgggcgg cctcctcatc atcaacaatc atcaatcgca acgcgtcttt tctcacttcc
     1381 gggtaagttt agagccttcg gcagtgaatt ccctagcgtg ttgcgtgcca cagtgggaca
     1441 ggcatttttg actgtctcgg aaactatagg caggaatgcc tgtcctactt tgccgtgcgt
     1501 ttgacggaga ccacgagaat gaccgcgtcg cccgatgggc cagcattgag cgtgcccacg
     1561 atcacgggct cgccttcacg catactgatg tccgtggaaa gagtggtccc ttcgtagctg
     1621 aagacgggcg gctccggatt ggcagccgca accgccgcgg cgttggctaa aatcggtatc
     1681 tgtgcattga aaacgaagcc ggtcatctgc accatcggct gtccttcgct gccgcgcaca
     1741 agcttgatgt gccgcactct gaacgaactc cgcgtcatta gctgagcagc actcggtgtc
     1801 gacgtcaacg gcgcaccaac ccaactgagc tcaagctttc cttcattccg cgaacgatta
     1861 atcaacgtgg cggcgagccg gtaatttttg aaaggcaacg ttgcgcgtag ttgtttgaca
     1921 actccgtcca gcgaactcgg aagcttggaa tcgaccacgt cctggttcgt acccagaatc
     1981 agataaagtt gtgtgtctac gtttgtctcg tcttctttgg tatccgcttg atttgactgc
     2041 gcctggacac tcaggccgcc aacgaacaga cacattccca ctaagatgaa cgtgagaaga
     2101 tttctaagtt tcattacgat ctccttcgaa caataattag gccacggata acacgcgagc
     2161 acatcgcgaa agttcccgct tgtcgtgcct agtcagaatt cttgttcgct acaggttctt
     2221 tctctttggt tttgatctct gttgcgacct ttactacttc gtccccgttc gtagtgccga
     2281 gctgtttgag tatctccggg acgttgagtc cgagtgcctg caactgttgc aggagtgcat
     2341 agatcatagt gggaccagtt tgagcgaacc ggtttaagcc gcccgaacca ccaccgtttc
     2401 cgtcaccacc atgatcgatc attacgacct tttcgatgtt tcctaacggt tcggaaatcg
     2461 cttttaacgc cggcgcggtc gcttcaatta tgcccggcag tctctcgagg atcgtctgca
     2521 gccgcgccgc gtcgttgaac tcgcgccagg cggccgcttt ctccttgatt gctgcggcct
     2581 ccgctaaacc ctgcgcctcg actgccttgg cttgcgctaa accttgcgct tcaatctttg
     2641 ctgcttcggc taggccaagc gcccgaattt tttccgcttc cgcacggccc tctgcttcga
     2701 tcgctgaagc acggcctgca ccctcgcgct gcaacttctg gctttcagct tctgcgctcg
     2761 ctatcaacgc ctgcttttgt ccttcggcct cgagaatcgc cgactcctgt tgtccttgcg
     2821 caagtagtac gcgagcgcga cgttcggctt cggctttctt aacaacggtc gcttccaatt
     2881 ctttctcgcg tcgcaatacc tcctgctctt gaacagagat ctgttcctgc gtgcgaattt
     2941 tttctacgcg aacttcctcg gctacgacct cttgccgcgc ttttgcttca gacaatggac
     3001 cagcctgctc cgcagtggct tgctcgcgtt tgatctcggc ctgatattgc gcccgcttta
     3061 cttcagtggc tctggttgaa gccgcaatct cagcttcgga agcgaggcga actcgttctc
     3121 cttcctgcat tgccagcgcc gctttgatct tcgagtcgcg cagtgcttcg gcctggccga
     3181 tctccgcgtc gcgctgcact tccgcagttc gcctcttgcc cagcgcgtcg agataacctt
     3241 cctcgtcaga gatctcctgg atcgtcaaag cgtcgagacc gatacccatc ttctccagat
     3301 ctccggcggc ctcagtggtt aacttctgcg caaagctctg acggtcgttg ttgatctcct
     3361 cgacagtcag cgtgcccaga atggcgcgca agtggccttc aagcgtctga aagacgagcc
     3421 tatgaaactc ttccggtttc attccgagaa agcgttccgc ggcagcgcgt aaagaatcat
     3481 cgtctccttt gatcttgacg ttggctacgg cctttacgga taccggaacg ccctgcaccg
     3541 tgtaggcgcg cgacgtggcg aggggcacgg tgagcacatt gaggttgagg tattcaacct
     3601 tttcgagaaa cggaacgatc aacgtcgcgc cgcccctgac aagtcgataa ccgacgaccg
     3661 tgccatcagg cagttttcgg gaacgtcccg agatgacagc ggcttgattg ggagaaactt
     3721 ttacgtagtt tcggctgagc agcatcaggg cgatcagaaa gccgagcact acaactagaa
     3781 tcacaatggg gatcagcagg ttcatgtcag gcattgcagg cacctcaaac taattttggg
     3841 tataggtaga atcgtaagga ctaacgatga gtgaatctcc ggcaacctca tcgacgcgaa
     3901 cagtggagcc ggcttttatc tcacggttat cgcgggcacg ggccaacttt tcaatgcgct
     3961 cttcaccgac tcgacaactc acctggccca ggcttccggg cgcgattgaa acg
//
LOCUS       DASTEY010000703         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1038215, whole genome shotgun sequence.
ACCESSION   DASTEY010000703 DASTEY010000000
VERSION     DASTEY010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038215"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VFO72_12180"
     CDS             <1..1413
                     /locus_tag="VFO72_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9480101.1"
                     /translation="SPDAIPRSREIGLDWTVLGFTAGVSFLTGILFGLIPAIQAGEVD
                     VHETLKETGRGVSGRHWLRSSLVVVEVATTLVLLIGAGLMIRSFYLLQKVNPGFSYEH
                     LTSFSVSLPEKKYTNQESRASFYNRLLENIRALPGVESAAAASGLPLGNNGWQTGFVV
                     DGQPVPPRDQTPLMEACLVTPDYFRAMNIPVLRGRAFTDGDDRSHLAGRDLSKLNENQ
                     AAIAALNSIVIDEEFAKRYWPNEDPIGKRIRLGADDDAQKLEVIGVVGRVKMESLNRN
                     SNRVQGYFPFAQVPNGGMTVIVKGTSEPNQLISSVRTAVKEIDPDQPIYNPRTMYEIR
                     AESVASERLNLTLLSIFAGIALVLAIVGIYGVMSYSVTQRTHEIGIRMAIGARPRDVF
                     TMILGQGMKLALIGVAIGLLLAFALTRLMETMLFGVEPTDVPTFAAISIMLISVAVLA
                     CYLPGRRATKVEPTISLRYE"
     gene            1564..2943
                     /locus_tag="VFO72_12185"
     CDS             1564..2943
                     /locus_tag="VFO72_12185"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9480102.1"
                     /translation="MPRILIADDQTDVVAALSLLLKGERFQIESASSPGGVLKAIDAQ
                     EFDVVLIDLNYARDTTSGSEGLDLLSKIRAVDPSLPVVVMTAWGSVDLAVEAMRRGAR
                     DFIQKPWDNARLLAVLRTQVELAEALRRGQRLEAENIALRDSVSDTGSEVPQIVAESP
                     AMKSVLKLVERIGPADANVLITGENGTGKEVITRVLHSISSRRSKPLISLNAGALAEG
                     VFESELFGHVRGAFTDAKSDRVGRFELADGGTLFLDEIGNVPLNLQAKLLRVIETGEF
                     ERVGSSKTQKVNVRLLTATNADLHAEVAQGRFRQDLLFRLNTVEIRLPPLRDRLEDIP
                     VLAEHFLKIHRERYRRPIVGFTPEALEALRHHLWPGNVRELDHVIERAVLMSTGNVVT
                     AFDLALEATPDARLSARLEELSLEDAERLLIKKALARFEGNANRAAEALGLSRSALYR
                     RLQKYGLGS"
     gene            2974..>3351
                     /locus_tag="VFO72_12190"
     CDS             2974..>3351
                     /locus_tag="VFO72_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9480103.1"
                     /translation="MSKPAKPKSHASLDPLLGRRGRLKFERHVTLLALAAGFPGVLLC
                     AFLLWYGNYSSQVQWTVDLFLVLLWLGISFNLKQRIVRPLQTLSNILAAIREGDYSIR
                     GRRALSGDALGEVMLEVNDLGQTL"
BASE COUNT          642 a          909 c         1047 g          753 t
ORIGIN      
        1 agtccggatg cgatcccgcg ttcgcgtgag atcgggctcg actggaccgt gctcgggttt
       61 actgccggcg tctcgtttct aaccggcatt ctgttcgggc tgattccagc cattcaagcg
      121 ggcgaagtcg acgtgcacga gacgttgaaa gagactgggc gcggtgtcag tgggcgacac
      181 tggttgcgaa gttcgctcgt tgttgtcgaa gtggcaacga cgttggtttt gctgatcggc
      241 gccggcttga tgattcgcag tttttatctg ctgcaaaaag tgaatccggg tttctcttac
      301 gaacacctga ccagtttcag cgtctctttg ccggagaaga aatacacaaa ccaggaatca
      361 cgcgcgtcgt tctacaatcg gttgctcgag aacattcgcg cgctgccggg agtggaatct
      421 gcggccgctg cgtcgggttt acctctgggc aacaacggtt ggcagaccgg gtttgttgta
      481 gacggacaac cagtcccgcc gcgcgatcag acgccactga tggaagcttg cctggtgacg
      541 ccggactatt tccgcgcgat gaacattccc gtgctgcgtg gccgcgcgtt tacggacggc
      601 gacgatcgct cgcatctcgc cggacgcgac ctgagcaagt taaacgagaa ccaggcggcg
      661 attgctgctt taaattcgat cgttatcgac gaagagtttg ccaaacggta ttggcccaac
      721 gaggacccga tcggcaaacg catcagactt ggcgccgatg acgacgcaca gaagcttgaa
      781 gttatcggcg tggttggccg cgtgaaaatg gaaagcctta atcgtaacag caatcgcgtc
      841 cagggatact tcccgtttgc gcaggtaccg aacggcggta tgacagtcat cgtcaaaggc
      901 acctccgaac caaaccagtt aatctcttcg gtgcgtacgg cggtgaagga gatcgatccc
      961 gatcagccga tttataaccc gcgcacgatg tatgagatcc gcgctgagtc ggtcgcgtct
     1021 gaacgtctga acctgacgct gttgagcatc tttgcgggca ttgcgctagt gctggcgatc
     1081 gttggtatct acggtgtgat gtcctactca gtcacgcagc gtacgcacga gatcgggatt
     1141 cgcatggcga tcggcgcgcg gccgcgcgac gtgtttacga tgatcctggg ccagggcatg
     1201 aaactggcgt tgatcggcgt ggcgatagga ttgttgctgg cgtttgcgct gacgcgtttg
     1261 atggagacga tgctcttcgg tgtcgagcca acggacgtgc cgacgtttgc ggcaatctca
     1321 attatgttga tttccgttgc ggtgctggcg tgttatctgc cgggacgccg cgcgacgaaa
     1381 gtcgagccga cgatctcgct gcggtacgag tagggccagg gattaacaca gatgagccct
     1441 gataaagccg ccgacattta cggcagcgcg ccacctttgc aatcgtctcc gttggactga
     1501 tcgttgtggc tttgttagct tgttacgtgc ctgcgcaaag agcaaatcga gccgcaaaca
     1561 cctatgcctc gcattttaat cgctgatgac cagactgatg tcgtcgcggc actgagcctg
     1621 ctgctgaaag gcgagcgttt tcagatcgaa agcgcgtcgt cgcctggcgg tgtgctcaaa
     1681 gcaatcgacg cgcaggagtt cgacgttgtg ctgatcgatc tcaactacgc gcgcgacacg
     1741 acttccggca gtgagggtct cgacttgctc agtaagatcc gcgcggtgga tccgtctctg
     1801 cccgtggtgg tgatgacggc ctggggcagc gtggatctgg cggtcgaagc gatgcgccgc
     1861 ggcgcgcgcg acttcattca aaaaccctgg gacaacgcgc ggttgctcgc cgttttgcga
     1921 acgcaggtcg aactcgccga agcgctgcga agaggacagc gtctggaagc ggagaacatt
     1981 gcgctgcgcg acagcgtctc cgataccggc agcgaggtac cgcagatcgt ggccgagtcg
     2041 ccggcgatga agtctgtgtt gaagctggtg gagcggattg gtccggctga tgcaaacgtc
     2101 ttgatcaccg gcgagaacgg caccggcaag gaagtgatca cgcgcgtact ccactccatc
     2161 tcgtcccgcc gttcgaaacc gctgatttcg ctgaacgccg gtgcgctggc ggagggcgtt
     2221 tttgaaagcg agctgtttgg tcacgtgcgc ggcgctttta cggacgcgaa aagcgatcgc
     2281 gtgggacgtt ttgagctcgc cgacggcggc acgctctttc tcgacgagat cggaaacgtg
     2341 cctttgaacc tgcaagcgaa attgctgcgt gtgattgaga ccggagagtt tgagcgtgtc
     2401 ggttcgtcga agacgcagaa ggtaaacgtg cgtttgttga cggcgaccaa cgccgatctg
     2461 cacgcggaag ttgcgcaggg ccgttttcgc caggatctgt tgtttcggct taacacagtc
     2521 gagattcgtt tgccgccgtt gcgcgatcgt ctcgaagaca ttccggtctt ggccgagcat
     2581 tttttgaaga tccatcgcga acgctatcgg cgtccgattg ttggtttcac gcccgaggcg
     2641 ctcgaggctt tgcgccatca cctctggccc ggaaacgtgc gtgaattgga ccacgtgatc
     2701 gagcgagccg tactgatgag taccggaaac gtggtgacgg cgtttgacct ggcgctggag
     2761 gcgacgcctg atgcgcgtct ctcggcgcga ttggaagagc tgagtttgga agatgccgag
     2821 cgcttgctga tcaaaaaggc actcgcgcgt ttcgagggta acgcaaaccg cgctgccgaa
     2881 gcgctgggct tgagccgtag cgcgctctat cgccggctgc aaaaatatgg cctcggctcc
     2941 tagtttgtgc ctctttgtgg ctatgattag cacgtgagta agcctgcgaa accgaaatct
     3001 catgcctcac tagatccgct actcgggcgc cgtggccggc tgaagttcga aaggcacgtc
     3061 acgcttcttg cgctcgcagc cggttttccc ggtgtcctac tgtgtgcctt tctgctctgg
     3121 tacggtaact actcctcgca ggtgcagtgg acggtcgatc tgttcctcgt tcttctgtgg
     3181 cttggaatat cgttcaatct caagcagcgc atcgtccggc cgctgcaaac tctctcaaac
     3241 attcttgccg ccattcgcga aggtgactat tccattcgcg gccggcgcgc gctctcgggt
     3301 gacgcgctcg gcgaggtgat gctggaggtt aacgatctcg gccaaacgtt g
//
LOCUS       DASTEY010000704         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_461022, whole genome shotgun sequence.
ACCESSION   DASTEY010000704 DASTEY010000000
VERSION     DASTEY010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461022"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..3149)
                     /locus_tag="VFO72_12195"
     CDS             complement(108..3149)
                     /locus_tag="VFO72_12195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9480104.1"
                     /translation="MTPALSRSIAAIVLFLAGLQVPVFAQQAGKSTLTGTITDPQGAV
                     VSGVKVIATLESAGVRRETVSNDQGLYVLTDLAPGEYELRVEAKGFITKVTKVPISLM
                     VGQSVTVDVPLEIGLSAEVIADDFSYEPRLIDSSASAVQGVIGSRDVENLPLNGRNFL
                     ELALLIPGNAPAPNFDPTKTNTVVISSAGQLGRGGNVTIDGADNNDDVVGGPVQNISQ
                     EAIQEFQIATNRYSAQLGRSGSSVINVVTKSGSNDLHGSASAYFRDRELQGLPATFDR
                     NLGQTPPFERQQYAFTLGGPIKRDRAWFFGSFEYRNQDGVVLVGARDLATRSIRRDFA
                     AAPLDDFLTTERVDWSPATNDHLSFRYSFQREKSVAASTLVRAIGSASQRQSSLNKTN
                     AFLANYSHIISPRDLNNFSFSFGTFINDTLPVAPGPQLTFPSLQDGASFRVPQQTKQR
                     RIQFSDTYTMVRGNHTFYFGGEVQRVESALDLKVFQQGRIELIEDFPDFDRDGDGRVD
                     DNDLLFAVTLRSGVPDRSLLLPHTDNTYLAGFVQDDWHVHRQFTLNLGLRYELDTDVK
                     NVSRVDELNPLILPFLRGERTRDRNNFAPRFGFNFSTSDGRTSVHGGYGIYYDRVTLE
                     IQTLERGLDGRALPVEVRAGNLFFIPPPFLFDPVNGVFPLGAPTLANPFTGFVLPGAG
                     AGGINIIDNDLQNPMVHQMNIGVQQELGRQFVLRADYLRNVGTHFIIGRIIGTVPFNP
                     VVGGPDLVKNLESSVRTKYDGLLVSLEKRFSKRYQFRASYTLSRSFNFANDDQIPFSN
                     GPIDSSNLRLEYGPTPNDQRHRFSFSGVFQLPKGIRVSPILTIASAVPIDILLPDGSS
                     RVCELERNAGARQFHTGAELNAALAQINAAGGSLCPNADPGTGFKPRVLLPLVRDDLR
                     LGDSFSSLDLRVSKVFKVGERFTIEPIAEVFNLFNVTNVLGLSNVNYSGFSNVLVRDS
                     NDPSSPAFLRSSSFGQPVSTAGGVFGSGGPRAFQFAARVTF"
     gene            complement(3189..>4500)
                     /gene="glmM"
                     /locus_tag="VFO72_12200"
     CDS             complement(3189..>4500)
                     /gene="glmM"
                     /locus_tag="VFO72_12200"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9480105.1"
                     /translation="VIGQSLTPRLLTRFAQAFGTHTGSGTIVVGRDPRTSGEMVKHAV
                     IAGILSSGGRVVDVGVCPVPTVQLQVRRRKAHGGIAITASHNPPEWNALKFIGSNGLF
                     LDAGQARELLDIYHQGEYTKVSGEEIRTVEEIEGAGDTHIKAVIDALGPLPASKRKVR
                     VVLDSCNGAGSIVGPKLLEALGAEVIPINVTPDGSFPRPAEPLPENLGDLCEAVKTHS
                     ADVGFAQDMDADRLAIVSEQGIPIGEDYTLVLAALYVLGRERGPVVANLSTTSALEDI
                     AKKFDCPLFLTKIGEVNVTDKMQQQHAVIGGEGNGGVIYPRINFARDSLVGMALVLHL
                     LAESGKSVSQLLDEIPRYSLVKKKMVCPSEKIPAVLRMLRQEYAQFPLDTRDGVKVTL
                     PEGWFLVRGSNTEPIIRVVAESKQESQAREIVARVYEQVAACIR"
BASE COUNT         1029 a         1364 c         1182 g          925 t
ORIGIN      
        1 gtgcgtaagc ctatctgtcg tttcagttca gccacggatt tgtcagcgcc gaaggcgctc
       61 gggctcgcac tcgaaccgca cgcaccttcg gcgcggctct tcgcgactta gaacgtcacg
      121 cgggcggcga attgaaatgc acgtggaccg ccggaaccaa aaacaccgcc cgcagtgctc
      181 accggttgcc caaaactcga cgaacgcaag aatgccggac tgctcggatc gttgctgtcg
      241 cgcaccagca cgttactaaa accggaatag ttcacgtttg aaaggcccag cacgttcgtc
      301 acgttgaaga gattgaacac ttccgcaatc ggttcaatcg taaaacgctc acccactttg
      361 aaaaccttcg acactcgcag atccagcgac gagaaactat cgccaagccg caaatcatca
      421 cgcaccagcg gcaacagcac gcgcggcttg aaacccgtgc ccggatccgc gttcggacag
      481 agcgaaccgc cagccgcatt gatctgtgcc aacgccgcgt tcaactccgc gcccgtgtga
      541 aactgtcgcg cgcccgcatt gcgctccaac tcacaaacgc gcgaacttcc gtccggcagc
      601 agaatatcaa tcggcactgc cgacgcaatc gtcagtatcg gcgaaacgcg aatccctttc
      661 ggcagttgaa acactcccga aaaactgaag cgatggcgct gatcgttcgg cgttggacca
      721 tactccaatc gcagattgct cgaatcgatc ggcccattcg aaaacggaat ctggtcgtcg
      781 ttcgcaaagt taaaggatcg cgacagagtg taagacgcgc gaaactggta acgcttcgag
      841 aaacgctttt cgagactcac gagcagtccg tcgtacttcg tacgcacgct cgactccagg
      901 ttcttcacga gatcgggtcc accgaccacc gggttgaacg gcaccgtgcc gatgatgcgg
      961 ccaatgatga aatgcgtacc aacgttacga aggtaatcgg cgcgcaaaac gaactggcgg
     1021 ccaagttcct gctgcacgcc gatgttcatc tggtggacca tcggattctg gagatcgttg
     1081 tcgatgatgt tgatgccacc ggcgcccgcg ccgggcagca caaaacccgt gaacggattc
     1141 gcgagcgtcg gcgcgcccag cggaaacaca ccgttaaccg gatcaaacaa gaacggcggc
     1201 ggaatgaaaa agagattgcc ggcgcgcact tcgaccggca gcgcgcggcc atcgagcccg
     1261 cgttcgagtg tttgaatttc cagtgttaca cggtcgtagt aaatgccgta accaccgtga
     1321 acgctcgtgc gcccgtcgct ggtggaaaag ttgaaaccaa aacgcggcgc gaagttgttc
     1381 ctgtctcgtg ttctctcgcc tctcaaaaac ggcaggatta gcggattaag ctcgtcgacc
     1441 cgactgacgt tcttcacgtc ggtatcgagc tcatagcgca aaccaagatt caacgtgaac
     1501 tgccggtgca cgtgccaatc gtcctgaaca aacccggcga gatacgtgtt gtccgtgtgg
     1561 ggaagcaaca acgaacgatc cggaacaccg ctgcgcaacg tcacagcaaa caatagatcg
     1621 ttgtcgtcaa cacgcccgtc gccatcgcga tcgaagtcag gaaagtcttc aatcaattcg
     1681 atgcgcccct gctgaaacac ctttagatcg agagccgatt cgacgcgctg aacttcgccg
     1741 ccaaaataga atgtgtgatt gccgcgaacc atcgtgtaag tgtcactaaa ctgaatgcgg
     1801 cgctgcttcg tctgctgcgg cacgcgaaac gaggcgccgt cctgcaaact cggaaaagta
     1861 agttgtggtc caggcgcaac cggcaacgtg tcgttgataa aagtgccaaa gctgaaactg
     1921 aagttgttaa ggtcacgcgg tgagatgatg tgtgagtaat tggcgagaaa cgcatttgtc
     1981 ttgttcagac tcgactgcct ttgcgacgcg gacccgatcg cacgcacgag cgtgctcgca
     2041 gcgacacttt tttctcgttg aaatgagtat cgaaaactca aatggtcgtt ggtggccggc
     2101 gaccagtcga ctcgttcggt ggtaagaaaa tcatccaaag gcgccgcggc aaaatcgcgt
     2161 ctgatactac gcgtggccag atctctggct cccaccaaca ccacgccatc ctgattgcgg
     2221 tactcaaacg aaccgaagaa ccacgcgcga tctctcttga ttggaccacc aagcgtaaac
     2281 gcatactgct gccgctcgaa cggcggcgtt tgccccagat tgcgatcgaa cgttgccggc
     2341 aacccctgca actcacgatc gcgaaaatag gccgatgcgg aaccgtgcaa atcgttggac
     2401 cctgatttcg tcaccacgtt gatgaccgac gatcccgaac gacctaactg cgccgagtaa
     2461 cggttcgtcg caatctgaaa ctcctgaatc gcttcctgcg agatattctg taccggacca
     2521 cccacgacat cgtcgttatt gtcagcgccg tcgatcgtga cattaccacc gcgtccgagc
     2581 tggccggcgg aggagatgac gacggtgttc gtcttggtgg gatcgaagtt gggcgcaggc
     2641 gcattgccgg gaatcaacaa ggccagttcg agaaagttac ggccattgag cggcaggttt
     2701 tcgacgtcgc gtgatccgat tacgccctgg accgcagacg cgctactgtc aatcaggcgc
     2761 ggctcgtagc tgaagtcgtc ggctataact tccgcggaaa gtccaatctc cagcggcacg
     2821 tccaccgtca ctgactgtcc gaccatcaat gagatcggca ccttggttac cttagttatg
     2881 aatcctttcg cttcgacacg cagttcatac tcgcccggcg cgagatccgt caggacgtag
     2941 agtccttgat cgttactgac cgtttcgcgt cgcacgcctg ctgattccaa agtagcgata
     3001 accttgacac ccgacacaac cgctccttgc gggtcagtga tcgtaccggt cagcgttgac
     3061 ttcccggctt gttgagcgaa cacaggcact tgaagaccag ctaaaaacaa gacgatcgca
     3121 gcgatggaac gcgataatgc aggggtcatt attgatcctt tcgtccgaaa acgtgtttat
     3181 ctgtggtcct acctgatgca ggcagcaact tgctcgtaga cgcgcgcgac tatttccctt
     3241 gcctgactct cctgtttaga ctcagccacc acgcgaatga tcggttcggt gtttgaacct
     3301 cgcacgagaa accaaccttc gggcaacgtc accttcactc catcgcgcgt gtcgagtgga
     3361 aactgcgcgt actcctggcg caacattctc aaaaccgcag gtatcttctc cgacgggcaa
     3421 accatctttt ttttaacgag actgtatcgc ggaatttcat ctaataactg cgacacgctc
     3481 tttccggatt cagcaagcag gtgaagcacc agggccatgc caactaaact gtcccgcgca
     3541 aaattgatcc gcggatagat cacgccgccg tttccctcac cgccgatcac cgcgtgctgt
     3601 tgctgcatct tgtcggtgac gttgacttcc ccgatctttg tcaggaacag cgggcaatca
     3661 aacttcttag cgatatcttc cagcgcggag gttgtcgata agttggcgac gactggtcca
     3721 cgttcgcgcc cgagcacgta aagcgccgcg agcaccagtg tgtagtcttc gccgatcgga
     3781 attccctgct cggaaacgat tgctaaacga tccgcgtcca tgtcttgcgc gaaaccgacg
     3841 tcggcgctgt gcgtcttcac cgcttcgcaa agatcgccga ggttctcggg caacggttcg
     3901 gccggacgcg gaaacgatcc gtcgggtgtc acgttgatcg gaatcacttc cgcgccgagt
     3961 gcttcgagca gctttggacc cacaatcgat cccgcgccgt tgcacgagtc gagcaccacc
     4021 cgcacctttc gcttgctcgc cggcaacggt cccagggcgt cgatcactgc cttaatgtgt
     4081 gtgtcgcccg ccccctcgat ctcttctacc gtccggattt cttcgccgct gactttcgtg
     4141 tactccccct ggtggtaaat atcgagcaac tcacgcgctt gtcccgcatc gaggaaaaga
     4201 ccattcgaac cgataaactt caatgcgttc cactccggcg gattatgact ggccgtaatc
     4261 gcaattccgc cgtgcgcttt gcgtcgcctg acctgcaatt gcaccgtcgg caccggacag
     4321 acaccaacgt ccaccacacg gccaccgctc gaaagaattc ccgcgatcac tgcatgtttg
     4381 accatctcgc ccgacgtccg cggatcacga ccaacgacga ttgttcccga tccggtgtgt
     4441 gtgccgaagg cttgcgcgaa acgagtcagc agccggggcg tcaacgattg gccgataacc
//
LOCUS       DASTEY010000705         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_1516199, whole genome shotgun sequence.
ACCESSION   DASTEY010000705 DASTEY010000000
VERSION     DASTEY010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516199"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..>2118
                     /locus_tag="VFO72_12205"
     CDS             373..>2118
                     /locus_tag="VFO72_12205"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9480106.1"
                     /translation="MRRLLIFILIFTFLLAPSAAQNERRPAVQIKGLKERVTITRDER
                     GIPYIDARSDEDLYFAQGYATAADRLWQMDLFRRTARGELAEVLGAGPNNVALNQDKL
                     HRTYGFAQAAEAEVAQASPKSLAVIEAYARGVNAYAASLDPKSLPPEFQLLQFNFRPW
                     NPADSFIIAKIFFEALSDTWRLDIMRQALSSLPEEKRRALLPEISPLDVLVVGKDTKV
                     RPASARLQPHSAPLSSEALAALAHNQAVAASALDRIGFYADGLAASNNWVVSGSRTVT
                     GKPLLADDPHLRPSAPSIWHMVHLTAPGMRVAGITAPGVPGVIIGHNERIAWGFTNVG
                     PDVQDVYIEKFDPENPRRYQTPQGWQDAVIRQEQIKVRKGVGSSEYDTVVHEVTVTRH
                     GPIIFQADGKRYALRWTALDPTKNRPDLSYGLNRARNWKEFKQSLETFTAPTQNIVYA
                     DVDGHIGYHAAGVVPIRKSGDGSVPYDGSTDAGEWTSYIPISKLPQLYDPPSGIIVTA
                     NQRIVGTDYPYFLTHSWATPYRARRIFDLLNEKQKHSIDDFRRIQGDVYSIAGVLFAQ
                     EAVKLLRPKLTASDEK"
BASE COUNT          483 a          601 c          588 g          446 t
ORIGIN      
        1 catcaggtgc tcgaacatat cgaagacgac ctcgcggcaa tgagcaaatt aaaccggatc
       61 ttgcggccgg gtagggtgaa cttgtggtgt cggtgccaat ggctaacgag gctgttactg
      121 tcgaatacgg cttcccggat ccaaaagagt caggtcattg gcggatgtac gggaacgatt
      181 tcgtaactaa gcttgaagcg agtggattcg aagtcaagaa agtcgatttt gtactgccag
      241 agaaaagacg cagcaaagtt cggcactagt gatgagccgt tttatctttg tcgaaaacgt
      301 gccgcaccct gaccgagcag cagacgatga acgggttaag cgtggagtgg caatatgcta
      361 gtatccgcac tcatgcgacg actcttaata ttcattctga ttttcacttt ccttctcgcg
      421 ccctcggctg cgcaaaacga acggcggccg gcggtacaaa tcaaaggact caaagaacgc
      481 gtaacgatca cacgcgacga acgcggcatt ccttacatcg atgccaggag cgacgaggat
      541 ctctatttcg cgcaaggtta cgcgacagcc gccgatcgtt tgtggcagat ggatctgttt
      601 cgccgtaccg ctcgcggtga actggctgaa gtcttgggcg ccggaccaaa caacgtcgcg
      661 ctgaatcagg acaaacttca tcgcacttac ggctttgcgc aggcagccga agcggaggtg
      721 gcgcaagcgt cgccgaaatc gttggcggta atcgaagcgt acgcgcgcgg cgtcaatgct
      781 tacgccgctt cgctcgatcc caaatcgctg ccgcccgagt ttcagttact gcaattcaac
      841 ttccgcccgt ggaacccggc agactcgttc atcatcgcca agatcttctt cgaagcgctt
      901 tcagatacgt ggcgactcga catcatgcgc caggcattgt cgtcattgcc ggaggagaaa
      961 cgccgcgcgc tattgcccga gatctcgcca ctcgacgtgc tcgtagtcgg caaagacact
     1021 aaagtgcgac cggcatccgc acgtttgcaa ccacacagcg ctccgctttc gtccgaagcg
     1081 ttagcagcat tggcgcacaa ccaggctgtc gcggcttcgg cgctggatcg catcggtttt
     1141 tacgccgatg gcctggctgc gagtaacaat tgggtagtca gcggcagtcg cacagtcacc
     1201 ggtaaaccgt tgctggccga tgatccgcat cttcgtccgt cagccccttc gatctggcac
     1261 atggtccacc tgacagcgcc cggaatgcgc gtggctggca tcactgcgcc gggcgtgccg
     1321 ggagtcatca tcggtcataa cgagcgcatt gcctggggtt tcacgaacgt cgggcctgac
     1381 gtccaggacg tctacatcga gaagttcgat cccgagaacc ctagacgtta tcaaacaccg
     1441 caaggctggc aggacgccgt cattcggcag gaacagatca aagtccgaaa aggagttggt
     1501 tcttccgagt acgataccgt cgttcacgaa gtgacggtga ctcgtcacgg cccaatcatc
     1561 tttcaggcag acggcaagcg ctatgcactc cgttggaccg cactcgatcc cacgaagaat
     1621 cgtcccgatc tttcgtacgg cttgaatcgc gcacgaaatt ggaaggagtt caaacagtcg
     1681 ctcgagacct tcacggcgcc aacgcagaac atcgtctacg ccgacgtcga tggtcacatt
     1741 ggttatcacg ccgcgggtgt cgtgcctatc aggaagtcag gcgacggtag tgtgccttat
     1801 gatggatcga ccgatgcggg tgagtggacc agttacatcc cgatatcaaa gctgccgcag
     1861 ctttacgatc cgccttcagg catcatcgtt actgcgaatc aacgaatcgt cggcaccgac
     1921 tatccctact tcctgacaca ctcatgggcg acgccgtatc gtgctcgtcg catcttcgac
     1981 ttgctgaacg agaaacagaa gcacagcatc gacgacttcc gccgcatcca gggtgacgtt
     2041 tattcgattg ccggagtgtt gtttgcgcaa gaggcagtca aacttttgcg gcccaagttg
     2101 acggcgtctg acgagaaa
//
LOCUS       DASTEY010000706         6437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U860
            k141_461367, whole genome shotgun sequence.
ACCESSION   DASTEY010000706 DASTEY010000000
VERSION     DASTEY010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37968867
            Sequence Read Archive: SRR25158423
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 09:12:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,446
            CDSs (total)                      :: 2,427
            Genes (coding)                    :: 2,421
            CDSs (with protein)               :: 2,421
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461367"
                     /isolate="SMAG_U860"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="35.37 N 114.9216667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="VFO72_12210"
     CDS             complement(<1..1689)
                     /locus_tag="VFO72_12210"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9480107.1"
                     /translation="MSTVTEVTSEATEQSTNKWVYLFDDGPGDDKALLGGKGAGLCEM
                     TQAGLPVPPGLVVTTEACNAFFDNDKNFPEGLWEQVQEGLKKIEEKVGKRFGDPKNPL
                     LVSVRSGAAFSMPGMMDTVLNLGLNEETVQGLAEQTGDLRFALDAYRRFASLFGEIVM
                     GVAHEKFERVLDRFKAQTQGGRDTDLNPDHLREIIAAEKQIILTEQRAIPEDPYEQLR
                     IAIAAVFNSWMGRRAIDYRRVNRISDALGTAVNVQAMVFGNMGPGSGTGVAFTRNPST
                     GKKELYGEYLLNAQGEDVVAGIRTPNPISQLKKELPEVYDQFASIAGLLEQHYKDMQD
                     CEFTIERGKLWMLQTRTGKRSGAAAVRIAVDMVYEKLIDKATAVQRVTPQQLDQLLHP
                     TVDPKTNATVLATGLPASPGAAQGQVVFSPDEAEEMAREDKRVVLVRQETSPDDFHGM
                     VAAQAIVTARGGMTSHAAVVARGMGKTCVSGAQAINIDYGQQQFTVDSTVVTKGEWIT
                     VDGSTGRVFLGQVPTIQPTLDNEFFKLMRWADQFRRLRVRANADTPLDAKTARQF"
     gene            complement(1958..2776)
                     /locus_tag="VFO72_12215"
     CDS             complement(1958..2776)
                     /locus_tag="VFO72_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9480108.1"
                     /translation="MVEATKARVAPVSSAMLYGRGVFTTLAIYNSNPFLWSRHWQRLS
                     THAAKLDIDHTGCTEKSVGEALKKLIAVNQVKDGRARVILLARSGRDLWKSKMPGGKK
                     TDLLIMTGDPPKAPPNGLSLAVSPYRTNTFSPLTGIKSLNYLNNVLAWEEAQSRDFDE
                     AVVLNERGEIASATTANLFWVKNGTVHTPALSTGAIAGITRECVIDIASKHFIPLLEG
                     VYEMADLTDADEIFLTASSLGVAPVTTFDFRRYAVEAGSVTTVIAQAFARLTSS"
     gene            complement(2816..5083)
                     /gene="purL"
                     /locus_tag="VFO72_12220"
     CDS             complement(2816..5083)
                     /gene="purL"
                     /locus_tag="VFO72_12220"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET9480109.1"
                     /translation="MITPEVISAHNLTPEEFERIKELLGREPSFTELGIFSVMWSEHC
                     SYKSSRVHLKRLPTSGQRVIVPPGENAGVVDLGDGWCAAFKIESHNHPSFIEPFQGAA
                     TGVGGILRDIFTMGARPIASMNSLRFGSLDHPQHGRRNRSLLAGVVAGIAHYGNAFGV
                     ATVGGEVAFDDAYALNPLVNAFALGLVRHDQIFFGKATGIGNPVLYVGAKTGRDGIHG
                     ATMASAEFDEEALEKRPTVQVGDPFLEKLLLEACLEAMRSGAVAGIQDMGAAGLTSSS
                     CEMAARAGTGIELDLSLVPQREQNMTAYEMLLSESQERMLVVAHAGREREVVDIFRKW
                     DLDAVVIGRVRDTQRMQVIHNGETVADIPVAALTDEAPRYERPMVPLTRESAPARDLA
                     DTDFTDALRILLSSGNVASKQWVYRQYDHMVRTNTAILPGADAAVVRIKETRRALAMT
                     LDGNGRYCAANPREGAKLIVAEAARNVVCTGARPIAITNCLNFASPERPEVMWSFSET
                     IDGMAEVCRAFDTPVVSGNVSFYNETEGRGILPTPVIGMVGLMEDVRRVVLPGFKKEG
                     DVIALLGVTQDDLSNSEYAVSVGGVTTAEIVASGKVPQLDLAREVAVQRACLDAAEVG
                     LLSSAHDCSDGGLAVALAESCFSSLGREAAGAEVELAATKHAGRALSVSELLFSESPS
                     RILISFERSATDAVRDIAAAHNAPFVVMGEVRGTRLRVQVDGNEVIGADVAELESAWR
                     NALLRSLKAEAIVAG"
     gene            complement(5345..5884)
                     /locus_tag="VFO72_12225"
     CDS             complement(5345..5884)
                     /locus_tag="VFO72_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9480110.1"
                     /translation="MRRSQVFLLIVLCVLLTSLAAPAQKRKNGAAKTPQIVVEPIDLE
                     ALKTLLTQPRQHPLLVNFWATFCDPCRDEFPDLVKIDKDYRPHSLEFVTVSLDDVSDI
                     KTTVPEFLGSMKATMPAYLLNVSDPEPAIDLVDPRWRGDLPATYLYNEKGEMVFRHIG
                     RVDAAKLRSEIEKLVKKGE"
     gene            complement(5888..>6437)
                     /locus_tag="VFO72_12230"
     CDS             complement(5888..>6437)
                     /locus_tag="VFO72_12230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9480111.1"
                     /translation="VLAGVAFANEVPPPPAIGSTVEDFTLPDLEGKEHSLKSLAGKNG
                     TVLLFVAVQCPVSNVYNERMEKLAQDYKAKGIAVIGINSNVAEDAAAVKAHAGEHKLT
                     FPILKDPGNKIADKLGASVTPEAYFLDANNKLLYHGRIDNSRNAAQVETSDLRNALDA
                     ALSGQPVEKAEARAFGCTIKRS"
BASE COUNT         1320 a         1944 c         1742 g         1431 t
ORIGIN      
        1 aaactgccgc gcggtcttag catccagcgg cgtgtcggcg ttcgcacgca cacgtaaccg
       61 ccgaaactga tcggcccacc tcatcagctt gaagaattcg ttatcgagcg ttggctggat
      121 ggtcggcacc tggccgagga acacgcgacc agtggatcca tcgacggtga tccattcgcc
      181 tttcgtaaca acagtcgaat ccaccgtgaa ctgctgttgg ccgtaatcga tgttgatcgc
      241 ttgagcgccg ctgacgcagg tcttgcccat gcctcgcgcc acgacagccg cgtgactggt
      301 catgccgccg cgcgccgtga cgatcgcctg cgcggccacc ataccgtgaa aatcgtccgg
      361 gctcgtttcc tgacgaacaa ggaccacgcg tttgtcttcg cgtgccatct cttcagcttc
      421 atcgggactg aacacaacct gtccctgcgc tgcgccggga ctggccggca aaccggtggc
      481 gagaacggtt gcgttggtct tgggatcgac ggttggatgc agcagttgat cgagctgttg
      541 cggagtgacg cgttggaccg ccgtcgcttt gtcgatcagt ttttcataaa ccatgtcgac
      601 ggcgatacgc actgccgcgg cgccgcttcg tttgcccgtg cgcgtttgca acatccaaag
      661 cttgccgcgt tcgatcgtga actcgcagtc ctgcatgtct ttgtaatgct gttcgagcag
      721 gcctgcgatt gacgcaaact ggtcgtaaac ctcaggcagt tctttcttga gttggctgat
      781 cggattcggc gtgcgaatac cggcgacgac gtcttcgccc tgcgcgttga gtagatactc
      841 gccgtagagt tcctttttac ctgttgacgg attgcgcgta aacgcgacgc cggttccgct
      901 gcccgggccc atgtttccga acaccattgc ctgcacgttc acggctgtcc cgagcgcgtc
      961 agaaatacga ttaacgcggc gatagtcgat cgcgcgacgt cccatccacg agttgaacac
     1021 tgcggcaatc gcgatgcgca gttgttcgta agggtcttcc gggatcgcgc gctgttccgt
     1081 caaaatgatc tgtttctcag cggcgatgat ctcgcgcagg tgatccggat tgagatcggt
     1141 gtcgcggccg ccctgcgttt gcgccttgaa ccgatcgagc actctttcaa acttctcatg
     1201 agcaacgccc atcacgatct cgccgaacaa cgaagcaaac cggcggtagg cgtcgagcgc
     1261 gaaacggaga tcgcccgtct gctccgcgag gccttgcaca gtttcttcgt tcaaaccgag
     1321 attgagtacc gtatccatca tgcccggcat tgagaacgcc gcgccggaac ggacggaaac
     1381 gagtagtggg ttttttggat cgccgaaccg cttgccgacc ttctcctcga tcttcttcag
     1441 accttcctgc acctgttccc acaaaccctc gggaaagttt ttgtcgttgt cgaaaaatgc
     1501 gttgcaggct tcggtggtga caaccaaacc cggcggcacg ggcaagccgg cctgagtcat
     1561 ctcacaaagg cccgcgcctt taccgcccag cagcgctttg tcatcgccgg gcccatcgtc
     1621 aaagaggtag acccacttgt ttgtactttg ttcagtagct tccgaggtta cttcagtaac
     1681 agtgctcatt gcggaacgac tctcttcctt ggattgaatg cttaaaaatg aaccaacgcg
     1741 tgcaaagtgc cagtgtcctg taaactccga aaccctgtca actgctgttt acttgggcgt
     1801 gctcttccct gggaccgcag gcgtctcgcc tgcaaggtga cgaatcgagc gctctcgtga
     1861 catgcaggcg agacgcctgc ggtcccaggg gaagagcgcg ttccggaaat cggcgcgcgg
     1921 ttcaagctgc ataactgtcc cacagattac acagattcta gctgcttgtt agcctggcga
     1981 atgcctgggc aatgaccgta gtgacgctgc ccgcttcgac ggcgtaccgg cgaaagtcga
     2041 aagtcgtaac gggcgccacg cccaacgaag acgcagttaa aaagatttcg tccgcatcgg
     2101 tcaggtctgc catctcgtaa acgccttcca gtagcggaat aaaatgtttg ctcgcgatgt
     2161 caataacgca ttcgcgcgtg atgccggcga tcgcgccggt gcttaacgcc ggcgtgtgga
     2221 cggtgccgtt cttcacccaa aacagattcg cggtggtggc tgatgcaatt tcaccgcgct
     2281 cgttcagcac caccgcttcg tcgaaatcgc ggctctgcgc ttcttcccag gcaagcacgt
     2341 tgttcaggta attcagagac ttgattcctg ttagtgggga aaaggtgttg gtgcgataag
     2401 gcgagactgc gagtgacaat ccgtttggcg gcgccttggg aggatcgcca gtcatgatca
     2461 gcaggtcggt cttcttgccg ccgggcattt ttgacttcca cagatcgcgt ccactgcgcg
     2521 ccagcagaat cacgcgcgca cggccatctt tcacctggtt cacggcgatc agtttcttca
     2581 acgcctcgcc gacacttttc tcagtgcagc ccgtgtgatc gatatcgagt ttggctgcgt
     2641 gcgtgcttag acgttgccag tgtctggacc agaggaatgg attggagttg taaatggcga
     2701 gagtggtgaa gactccgcgg ccgtagagca tggcagacga gaccggcgcg acccgcgcct
     2761 tagtcgcttc aaccatggat ttattcagat agatgaccgg atgcacagca cttttttaac
     2821 cggcgacgat tgcttcagcc ttgagcgatc gtaacagtgc gttgcgccag gctgactcaa
     2881 gctctgcgac gtctgcgccg ataacttcat ttccatcaac ctgtactcgc agtcgcgttc
     2941 ctcgaacttc acccattacg acaaacggag cgttgtgagc ggcggcgatg tcgcgcacag
     3001 catcggtggc actgcgctca aaactgatta ggatccgcga cggagattcg ctaaacagaa
     3061 gttcacttac actgagtgcg cggcccgcgt gctttgtcgc cgcgagttcc acctccgcgc
     3121 ccgcggcttc tcttccaagt gacgaaaagc atgactcggc caacgccacc gccagacctc
     3181 cgtccgagca gtcgtgcgca gaagacaaaa gccctacttc cgcggcgtcg aggcacgctc
     3241 gttggactgc gacttcgcgc gccagatcca gttgaggcac tttcccgcta gcgacaatct
     3301 ccgccgtcgt tactccacca acacttactg catattcact gttcgaaaga tcgtcctgtg
     3361 tcacacctaa cagcgcgatc acgtcgccct cttttttgaa gcctggcaac acgacccgtc
     3421 tgacgtcttc catcaaaccg accattccaa tcaccggtgt cggcagaatg ccgcgaccct
     3481 cagtctcgtt gtagaaggag acgttgccgg aaacgactgg cgtatcaaat gcgcggcaaa
     3541 cttcagccat gccgtcgatc gtttccgaga acgaccacat cacctccggc cgctcaggcg
     3601 aggcaaagtt gagacagttc gtgatcgcga tcggacgtgc accggtacaa accacgttgc
     3661 gcgcagcctc cgccacgatc aacttcgccc cttcgcgcgg attcgccgcg cagtagcggc
     3721 cgtttccgtc gagcgtcatc gcgagcgcgc gacgcgtctc ttttattcga accacggcag
     3781 cgtcagcacc cggcagaatc gctgtgttcg ttcgaaccat gtggtcgtac tgtcgataca
     3841 cccattgctt cgacgcgaca tttcccgaac tcagcaaaat ccgtaacgca tccgtaaaat
     3901 ccgtgtccgc taagtctcgc gccggcgcac tctcgcgcgt aagcggaacc atcggccgct
     3961 cataacgcgg cgcctcatcc gtcaacgccg caaccggaat atcagccaca gtctcgccgt
     4021 tgtgaattac ctgcatccgc tgcgtgtcgc gaacgcgccc gatcacgacc gcgtcaaggt
     4081 cccacttgcg aaagatgtcg acgacctcgc gctcgcgacc cgcgtgtgcc accaccaaca
     4141 tccgctcctg cgactccgag agcagcattt cgtacgccgt catgttctgt tcgcgttgcg
     4201 gcacgagtga aaggtccaac tcgatccccg tgcccgcacg cgccgccatt tcacatgaag
     4261 acgacgtcaa acccgccgcg cccatgtctt gaattcccgc cactgcgcca ctgcgcatcg
     4321 cttccaaaca agcctccaac aaaagctttt ccagaaacgg atcgccaacc tgcactgtcg
     4381 gacgtttctc caaagcttct tcgtcaaatt cagcggaggc catcgttgca ccgtgaatac
     4441 cgtcacgtcc ggtcttcgcg ccgacgtaca gaactggatt cccaatccca gtcgctttgc
     4501 cgaagaagat ctggtcgtgt cgcacgagac cgagcgcgaa cgcgttgacg agtggattca
     4561 aagcgtaggc atcgtcgaag gccacttcac cgccgacagt cgcgacgccg aacgcatttc
     4621 cgtaatgtgc aataccggca acgacgcccg ccagtaacga tcgattacgc cgcccatgct
     4681 gcggatggtc cagtgaacca aaacgcaaac tgttcatcga cgcgatcgga cgtgcaccca
     4741 tggtgaagat gtcgcgcaag atgccgccaa caccggtcgc tgcgccctga aacggttcga
     4801 tgaaagaggg atgattgtgc gattcgatct tgaacgcagc gcaccagccg tcgccgagat
     4861 cgaccacgcc cgcattctcg ccaggcggca cgattacgcg ctggccgctg gtcggcagtc
     4921 gcttcaagtg cactcgcgac gatttgtagg agcagtgttc agaccacatg accgagaaga
     4981 ttccgagttc cgtaaaactc ggttctcggc caagcaactc ttttattcgc tcgaattctt
     5041 cgggagtgag attgtgcgcg gagataactt caggtgtgat catttttgag ctttgttctt
     5101 tgcacctcgt tcaagtcttg tgcgctgtcg ctgtcttgtg cgcttcgctg atctgtgcgc
     5161 tgtcgctgtt ctttgctccg caggagcaaa atgtctatag cttttgcttg gctcctccat
     5221 cgcgctccca aggagcccaa cgtctcttta tgcccgaggc tatagacatc ttgctccggc
     5281 ggagcaaggc agcagtacaa gcacagcaaa aaacaaagaa caaagcacta agaacaaaga
     5341 actgctactc acctttctta accaactttt cgatctcgct gcgcagtttc gctgcatcca
     5401 cacgaccgat gtgcctgaaa accatttcac ccttctcgtt gtagagatat gtggccggta
     5461 gatccccacg ccatcttgga tccaccaggt cgatggccgg ctcaggatcc gaaacgttca
     5521 gcaggtaagc gggcatcgtc gccttcattg aaccgagaaa ctccggcacg gtggttttaa
     5581 tgtcgctcac gtcgtcgagg gagacggtta cgaactcgag cgaatgcggc cggtagtctt
     5641 tatcgatctt caccaggtca ggaaactcat cgcgacacgg atcgcagaac gtggcccaaa
     5701 agttcaccaa caacggatgc tgtcgcggct gcgtcagcaa cgttttgaga gcctcgaggt
     5761 cgattggttc aacgacaatc tgcggcgtct tcgcggcgcc gttctttcgc ttctgagccg
     5821 gcgccgcgag actggtcaat agcacgcata aaacaataag tagaaagact tgagaacgac
     5881 gcattgctta actccgcttg atggtgcaac caaatgccct ggcttccgct ttttcaaccg
     5941 gctggccact gagtgctgca tcgagtgcgt tacgcagatc gcttgtttcg acctgcgctg
     6001 cgttgcgcga gttgtcgatg cgtccgtgat agagcagttt gttgtttgcg tcgaggaagt
     6061 atgcttccgg cgttacgctc gcgccgagtt tgtcggcaat tttgttgccg ggatctttca
     6121 gaatggggaa ggtgagcttg tgctcgcccg cgtgtgcttt tacggcggct gcgtcttcgg
     6181 cgacgttgga attgatgccg atcactgcga tgcctttagc tttgtagtcc tgagccagct
     6241 tttccattcg ctcgttgtac acgttggaaa cgggacactg cacagcgacg aacagcagga
     6301 ccgtgccgtt cttgcctgcc agagacttca gcgaatgctc ctttccctcg aggtcgggca
     6361 gagtaaaatc ttcaaccgtg gagccaattg caggcggcgg cggaacttcg tttgcgaagg
     6421 ccacgcccgc aagcaca
//
